LOCUS       DASVEU010000001         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_388870,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000001 DASVEU010000000
VERSION     DASVEU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388870"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF225_00005"
     CDS             <1..248
                     /locus_tag="VF225_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143665.1"
                     /translation="PGSKGGNVVPTQSPGPVVNNPSDPDNPTRGRSVGQFKQDGSTSR
                     LFAALPSATEPSRSCGRARSPAAGASTSPFESFLGKL"
     gene            complement(245..445)
                     /locus_tag="VF225_00010"
     CDS             complement(245..445)
                     /locus_tag="VF225_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143666.1"
                     /translation="MARDVLDAKGGCFMANGDNRMFGIGLGGVLVIAGILLALLWSVW
                     VGVIVALVGLVAFGGFAKGRWY"
     gene            complement(451..1287)
                     /locus_tag="VF225_00015"
     CDS             complement(451..1287)
                     /locus_tag="VF225_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX7143667.1"
                     /translation="MSRLPDSPVHAAKGAGRDFVYSTAFEVLSRAGFVARGVVYGIIG
                     VLAFLVAVSDKGKLTDQEGAIRTLADEPFGTTLLVVLAIGLGGYSIWRLFRAALGHGP
                     EASDSTIDRLGGVGSGLVYGGLCIATIKILTGSSSSGAGSTPDKTTAGVFDWPAGRWL
                     VGIAGLVLIGVGAYQFLKGARQKFLEELKTEQMAPETKRWITRIGTVGHIARAVVFAL
                     IGWFLLKAAYEFDAKEAIGLDGALTKIVTASYGPWLLGLVSAGLIAFGVYSVSESRYR
                     KI"
     gene            complement(1349..2197)
                     /locus_tag="VF225_00020"
     CDS             complement(1349..2197)
                     /locus_tag="VF225_00020"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX7143668.1"
                     /translation="MNARWLAALSLLPLAAVLLVSTALAGQGEEMRGSLAKLRGVNFV
                     SSCTFSHAASDDPIVFFGKPGASHHHSFVGNTGTHASSTLESLLAGGSSCHRPGDTAG
                     YWMPTLYDNGRPVMPNHAQIYYRRKTMRHVEAFPPGFRMVAGDAKATAPQPLRIAFWN
                     CGVGGGVSPTSAVPTCPDTRRNALRLHVTFPSCWDGKNLDSASHQSHVAYPVAGRCPG
                     RFSHALPQISLIYRYPVTGGSGVTLASGGQLSGHADFFNAWRQSDLQSLVDRCLNALR
                     HCQRGA"
BASE COUNT          439 a          868 c          883 g          379 t
ORIGIN      
        1 cgccgggcag caagggcggg aacgtcgtcc cgacgcagtc gccgggaccg gtcgtgaaca
       61 acccgtccga cccggacaac ccgacgcgcg gccgttcggt cggtcagttc aagcaggacg
      121 gatcgacatc acggctcttt gccgcgctgc cttcggcaac tgagccgagc cgctcttgcg
      181 gccgggctcg ctccccggcc gcaggagcga gcacgtcgcc gttcgagtcg ttcctcggga
      241 agctctagta ccagcggccc ttggcgaacc cgccgaacgc gacgagccct acgagagcga
      301 cgatgactcc gacccagacg ctccagagga gcgcgagcag gattcccgcg atgacgagga
      361 ctccgccgag accgataccg aacatcctgt tgtcgccgtt cgccatgaag cagcctccct
      421 tcgcgtcgag aacgtccctg gccaaccgcc ctagatcttg cggtagcgcg actcgctgac
      481 cgagtacacg ccgaaggcga tcaagccggc cgagacgaga ccgaggagcc aggggccgta
      541 gctcgcggtg acgatcttcg taagcgcccc gtcgaggccg atagcctctt tcgcgtcgaa
      601 ctcgtaggcg gccttgagga gaaaccagcc gatcagcgcg aagacgaccg cgcgggcgat
      661 gtggccgacc gtgccgatgc gcgtgatcca ccgcttcgtc tccggtgcca tctgctcggt
      721 cttgagctcc tcgaggaact tctgccgggc gcccttgagg aactggtagg cgccgacgcc
      781 gatgaggacg agcccggcga tgcccacgag ccatcgtccg gcgggccagt cgaagacacc
      841 ggcagtcgtc ttgtcgggtg tagagccggc gcccgagctc gacgagcctg tgaggatctt
      901 gatcgtggcg atgcagaggc cgccgtagac gagcccgctc ccgaccccgc cgaggcgatc
      961 gatcgtgctg tcggacgcct cgggaccgtg cccgagggcg gcgcggaaga gtctccagat
     1021 cgagtagccg cctagtccga tcgcgaggac gacgagcagg gtcgtaccga acggctcgtc
     1081 ggcgagcgtc ctgatcgcgc cctcctggtc ggtgagctta cccttgtcgc tcacggcgac
     1141 gaggaacgcg agcacaccga tgattccgta caccaccccg cgggcgacga agccggcccg
     1201 cgagaggact tcgaaggcag tggagtagac gaagtcgcgg ccggcaccct tcgcggcgtg
     1261 aacgggtgag tctggaaggc gcgacatcgc ggggccattc ctaccccgtt atcggccgac
     1321 tcactcctgg atcgcgatca gcgtcgcgtc aggcgccgcg ctgacagtga cgcagcgcat
     1381 tcaggcagcg gtcgacgagc gattgcagat cgctctgccg ccaggcgttg aagaagtccg
     1441 catgaccgga gagctgaccg cccgaggcga gcgtcacccc gctcccgccc gtcaccggat
     1501 agcggtagat gagcgagatc tgcggtaggg cgtgcgagaa cctccccggg cagcgacccg
     1561 ccacggggta ggcgacgtgg ctctggtggg acgcgctgtc gaggtttttc ccgtcccagc
     1621 agctcgggaa cgtgacatgg aggcggagcg cgttcctacg cgtgtcggga cacgtcggca
     1681 cggcgctcgt cggcgagacg ccaccgccga cgccgcagtt ccagaacgcg atccggagtg
     1741 gctgcggcgc ggtcgccttc gcgtcacccg cgaccatcct gaagccgggc gggaacgcct
     1801 cgacgtgccg catcgtcttc cgccggtagt agatctgcgc gtggttcggc atcaccggcc
     1861 gcccgttgtc gtacagcgtg ggcatccagt acccggccgt gtcgccgggc cggtggcagc
     1921 tcgagccacc ggccaggagc gactccagcg tcgaggacgc gtgcgtcccc gtgttcccca
     1981 cgaacgaatg gtggtgcgac gcaccgggct tgccgaagaa gacgatcggg tcgtcactcg
     2041 ccgcgtggct gaacgtgcag gagctgacga agttgacgcc gcggagcttc gcgagcgaac
     2101 cgcgcatctc ctccccctga cccgcaagcg ccgtcgagac gagcagaacg gctgccaggg
     2161 gcagaagcga gagtgcggcg agccagcggg cgttcatgcg agcacctccg agcggcgggg
     2221 acgcgccgat cgaatcaccg ctctgtgtgg atttcgtgtg cgctgcgtaa gccgaactgc
     2281 agaatcggtg ccagacactg ttgcctgtca agcggaaacc gtgccgatcg tgtgccgatg
     2341 tatccccagg ccgaccctgg tcacatggtc gcccaagcga cgggcggtgt cgttcgtgtc
     2401 acgatcgtgc cgatcgctcc acaggttgac agggagcggt gcctgacacc ggtgttcagc
     2461 gggccgtcca gccgccgtcc atgacgagcg gcgcgcccgt gaacgcacgt cccgtgtcgg
     2521 cgaggagaaa ggcgaccgct cccgcgacct cggccggctc gaggagccg
//
LOCUS       DASVEU010000002         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1166965,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000002 DASVEU010000000
VERSION     DASVEU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166965"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VF225_00025"
     CDS             <1..1533
                     /locus_tag="VF225_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7143669.1"
                     /translation="LYTQEDVGPLDAIGFPGEYPFTRGVYPSMYRGRLWTMRQFAGFG
                     TAEETNERFRYLLDHGQTGLSTAFDMPSLMGHDSDHVRSEGEVGREGVAIDTLDDMET
                     LFSGIDMGEVSTSMTINAPAAVMLAFYVVAAERQGIGPERLAGTIQTDILKEYIAQKE
                     WCFPVDPAMRLVTDMIEFCARRMPRWHPVSISGYHIREAGSTAQQELAFTLKDGFTYV
                     ELAIERGLDVDEFAPRLSFFFNAHIDFFEEIAKYRAARRIWARELRDTYGARDERSLL
                     MRFHTQTAGVSLTAQQPLNNIVRTATEALSAVLGGTQSLHTNSYDEALALPSEEAVHV
                     ALRTQQVIAHETGVTNTIDPLGGSYFVEALTDRLEEAAYVEFSKIDELGGMVEAIKQS
                     YPQREIAEASFRLQEEIERGERVIVGVNRYQQEDERQLEILRIPAELERKQIGRVQAV
                     RAAREGAAVETALAELRDAAAADRNLMEPLLECARAHASEGEIVQSLQQVFGTYTETP
                     VF"
     gene            1635..>2234
                     /locus_tag="VF225_00030"
     CDS             1635..>2234
                     /locus_tag="VF225_00030"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HEX7143670.1"
                     /translation="MTPRLTRIAITAVAVAVSLGLAPSAFAATESGDAGDLPSTAQDL
                     GSGAVTSIFGSFTGGADVDVYRVCLTNGVSFSATTVGATTLDTQLFLFDSNGRGVYSN
                     DDASLGIHGSRLPSAHAFSPSGPGEYFIAISSFNNDPQSDAGEIFPDLFSSSLYPDGV
                     VPAVGMGGEEPVVSWVGPTRGPPGLYRINVTGTMGCDMTA"
BASE COUNT          379 a          759 c          758 g          338 t
ORIGIN      
        1 ctctacacgc aggaggacgt gggcccgctg gacgcgatcg gcttccccgg cgagtatccg
       61 ttcacccgcg gcgtgtaccc gtcgatgtac cgcggccggc tctggacgat gcgccagttc
      121 gcaggcttcg ggaccgcaga ggagacgaac gagcgcttcc gctacctgct cgaccacggg
      181 cagacggggc tgtccacggc gttcgacatg ccgtcgctaa tgggtcacga ctccgaccat
      241 gtgcgctccg agggcgaggt tgggcgcgag ggcgtggcga tcgacacgct cgatgacatg
      301 gagacgctgt tctcggggat cgacatgggc gaggtctcga cctcgatgac gatcaacgcg
      361 ccggcggcgg tgatgctggc gttctacgtg gtggcggcgg agcggcaggg catcggcccg
      421 gagcggctcg cgggcacgat ccagacggac atcctcaagg agtacatcgc gcagaaggag
      481 tggtgcttcc cggtcgaccc ggcgatgcgg ctcgtgaccg acatgatcga gttctgcgcc
      541 cggcggatgc ccaggtggca tccggtgtcg atctcgggct accacatccg cgaggccggc
      601 tcgacggccc agcaggagct tgcgttcacg ctgaaggacg gcttcaccta cgtcgagctg
      661 gcgatcgagc gcggcctcga cgtggacgag ttcgcgccgc gtctgagctt cttcttcaac
      721 gcccacatcg acttcttcga ggagatcgcg aagtaccgcg ccgcccgccg tatctgggcg
      781 cgcgagctgc gcgacaccta cggcgcgcgc gacgagcgct cgctgctgat gcgcttccac
      841 acccagacag ccggggtgtc gctcaccgct cagcagccgc tcaacaacat cgtgcgcacg
      901 gccaccgagg cgctttcggc agtgctcggc ggaacgcagt cgctgcacac gaactcgtac
      961 gacgaggcgc tcgcccttcc gagcgaggag gccgtgcacg tggcgctgcg gacccagcag
     1021 gtgatcgcac acgagaccgg cgtgaccaac acgatcgacc cgctcggcgg ctcgtacttc
     1081 gtggaggcgc tcaccgaccg cttggaggag gcggcgtacg tcgagttctc gaagatcgac
     1141 gagctgggcg gcatggtcga ggcgatcaag cagagctacc cccaacgcga gatcgccgag
     1201 gccagcttcc gcctccagga ggagatcgag cgtggtgagc gcgtgatcgt gggagtgaac
     1261 cgctaccagc aggaggacga gcgccagctc gagatcctgc gcatccccgc tgagctcgag
     1321 cgcaagcaga tcggccgcgt gcaggccgtg cgcgccgcgc gcgagggggc ggctgtggag
     1381 acggcgctcg ccgagctcag ggacgccgcc gcggctgacc gcaatctgat ggagccgctg
     1441 ctcgaatgcg ctcgggcgca cgcttcggag ggcgaaatcg tgcaatcgct gcagcaggtt
     1501 ttcgggacgt acactgaaac tcccgtcttc taggccaatc cggggcgtcc agacggacgg
     1561 ataggcagta acggacggcc cccgaacggc cgattgagac agcagttccc tccgtgccgc
     1621 gggaaaggaa acccgtgaca ccacgtctca ccagaatcgc catcacagca gtagcagtcg
     1681 ccgtttcgct cgggcttgcg cccagcgcgt tcgccgctac cgaaagcggc gacgccggcg
     1741 atctgccatc gaccgcgcag gacctcgggt ccggcgccgt cacgtcgatc ttcggcagct
     1801 tcacgggtgg cgcggacgtg gacgtctacc gggtgtgtct caccaacggc gtgtccttct
     1861 cggccacgac ggtcggcgcg acgacgctcg acacgcagct cttcctcttc gactcgaacg
     1921 gccgcggcgt ctactcgaac gacgacgcca gcctcggcat ccacggctcg cggctcccca
     1981 gcgcgcacgc cttctcgccg tcgggcccgg gcgagtactt catcgccatc agctcgttca
     2041 acaacgaccc gcagagcgac gccggcgaga tcttccccga cctcttcagc tcgagcctgt
     2101 acccggacgg cgtggtgccc gcagtcggaa tgggaggcga agagccggtc gtgagctggg
     2161 tcggacccac ccgcggtcca cccgggctct accgcatcaa cgtgaccggc accatgggct
     2221 gcgacatgac ggcg
//
LOCUS       DASVEU010000003         8751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_390313,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000003 DASVEU010000000
VERSION     DASVEU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390313"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF225_00035"
     CDS             complement(<1..287)
                     /locus_tag="VF225_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7143671.1"
                     /translation="MTSVETPSRLELEIGGMSCASCAARVEKELNQLEGVSASVNYGT
                     ELASVAYDPGHVHLPDLVRTVEEAGYTAALPSDADAEADPVRPLRLRLLVS"
     gene            complement(324..2303)
                     /gene="ftsH"
                     /locus_tag="VF225_00040"
     CDS             complement(324..2303)
                     /gene="ftsH"
                     /locus_tag="VF225_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7143672.1"
                     /translation="MNRFFRSALFPLVIIAALVWLALQTLGGHSQPTSKLTLSQVQQK
                     IEACNTVAGSGCDFAQIVFNPNKQALNYTLTDKTKGSVHYPSDQSAADLQKLMAQHNV
                     NFDSKGTGGSPWWSILTSLLPFVLLFGFWIFLMNQVQGGGSKVMSFGKSRAKRMTPDS
                     PKIGFKDVAGVEEAVEELQEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARA
                     VAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQASPCIVFMDEIDAVGRHRGA
                     GLGGGHDEREQTLNQLLVEMDGFELKDNIILIAATNRPDILDPALLRPGRFDRQIVVD
                     RPDRNGRSKILSVHTKGKPLASEIDLDTLAAGTPGFTGADLANLVNEAALLAARRGKK
                     VIEQEELEEGIMRVIAGPEKKTRLFSEEERKITAYHELGHAIVGHYLDGESDVHKISI
                     IGRGQALGYTISLPREDRYLTTKQTLMNQLAMTLGGRAAEEIVFNEVTTGAANDLEKV
                     THTAKQMIMRFGMSEKLGPRVLGRNHDMPFLGREMGNEPDYSEEIAREIDDEIRRVIE
                     EAHMTATEVLKEHSDELHRISLILIERETIDKDQFERLLAGEDEEAVFAEKTPAPKTE
                     PEPEAEKKRKPEPKTRPFPIPGATMQPPPPEGAKG"
     gene            complement(2377..2937)
                     /gene="hpt"
                     /locus_tag="VF225_00045"
     CDS             complement(2377..2937)
                     /gene="hpt"
                     /locus_tag="VF225_00045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7143673.1"
                     /translation="MSQTELERGVSAVLIDEDRLRSRVVELGEEISADYAGRDLLLIG
                     VLKGAVFFMADLMRTLAIPCEIDFMAISSYGAQTDSSGVVRILKDLDINIEGRNVLVV
                     EDIIDSGLTLSYLMRNLEAREPATLEVCALLTKPDRREIDVPVRYIGFEIPNRFVVGY
                     GLDFAERYRNLPYVGVLDSELVPEGA"
     gene            complement(2924..3895)
                     /gene="tilS"
                     /locus_tag="VF225_00050"
     CDS             complement(2924..3895)
                     /gene="tilS"
                     /locus_tag="VF225_00050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7143674.1"
                     /translation="MDTERLRERVEELVRRHDLIAPAGDVTCLVSGGADSTCLWHVLT
                     ELGYRVSALHVNHGLRGAASDEDARFCAERFGAEVVDGSGGLTEDDWRRSRYSFATDR
                     LRATGHTASDQVETVLYKLVASGVAKGIKVRREDGVVRPLLSVWRAETEEYCRAEGLE
                     FRTDESNSDTKRGLIRDEILPRLRELHPAAESNLLRLAEGEPTALDELLASTAGSRRL
                     DLGAGLTAVREYDRVWLERTPVALDGEVRWGEWRIASRLPGLKVRAWRAGDRLAGRSK
                     KIQDVFVDAKIPRSARESWPLVVRGDDVVAVPGIVEAEGVRVERVPD"
     gene            3956..4234
                     /locus_tag="VF225_00055"
     CDS             3956..4234
                     /locus_tag="VF225_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143675.1"
                     /translation="MAEFFQAWRKRQSEKQAAEWTEEQEEARRAVQELPDVLRDRARL
                     AVEKLIDGPDAEVEEALEELDHLLEPHPDLKDRIFRLRVVDDAVEFLK"
     gene            complement(4235..4708)
                     /locus_tag="VF225_00060"
     CDS             complement(4235..4708)
                     /locus_tag="VF225_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143676.1"
                     /translation="MKPRITAAAGLAMAIFVVASFVVLDNTGTSKAAVSHTCSATDRQ
                     FLGAAELNMAALGTLSQDYLQGDAKADDVILQTDSAVTSLLNTNPSDPSLSKTQKILR
                     AMFLEYGRAIRADKHHHDPGKYIYRAYGLANFAHDILSQAKPALAKRGCDVSPLL"
     gene            complement(4848..5627)
                     /locus_tag="VF225_00065"
     CDS             complement(4848..5627)
                     /locus_tag="VF225_00065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7143677.1"
                     /translation="MKGAIEPRLEQILEFCAEDPVERVFLEDVARRRLGRFSAFADNG
                     RLTALCHVGANVVPSGSECGRFAQAASRGRARMIIGDEHAVDALWDDLARLMPAPRND
                     RPGQPVYAISEPPTAGDTGLRPATDKDFPLLLPASAAAHEEEIGVDPMARDPEGFRWR
                     TRSQIEEGRSWLWVERGTILFKAEASAWTPEAVQVQQVWVDPPARRKGYAQRGMRDLC
                     RLLLERVPTVCLFVRPENAPAIRVYEAIGMQRHGSYRSLIF"
     gene            5664..6383
                     /locus_tag="VF225_00070"
     CDS             5664..6383
                     /locus_tag="VF225_00070"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HEX7143678.1"
                     /translation="MPIHIRAEPGEYADACLLPGDPLRAKYIAETYFDNPVEKNSERG
                     LLGYTGTYDGKPVSVQGTGMGCPGATIVFEELIQLGVKKLLRVGTCGGLQAHHELGDL
                     IVALSAVADDRTADHLVGHEPHCPTASWELIHGAVHTAKQMGQSMHVGPIVSSDVFYN
                     PDENQYERWSKRGVLAVEMEASALFTVGALRGVQSGCLLTVSDIVVEGEFKRITDEEL
                     RAAVDRMTRVALATVTADEKH"
     gene            6398..7291
                     /locus_tag="VF225_00075"
     CDS             6398..7291
                     /locus_tag="VF225_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7143679.1"
                     /translation="MINPASANGSTGRRWPEIARRAAAAGLTGDALFSRSAGELTELA
                     GRAADEGAELLVVVGGDGSVHEVANGIAGRSEVEIAVIARGTGWDFARSLGLPRNIDR
                     AIDVALSGETRTIDLGRATYRLWAGGEGQSFFANVASAGMSGAIAQRANDSSKALGGK
                     VSYVWATFAVFARWSSSEIEVSVDGETRRALMHDVIVSNGPYVGGGMKICPDAELDDG
                     IFDVLLIGDLTKRDLVLTMPKIYRGTHLPHPKAELLRGSQVTVDAADPLPIELDGEQP
                     GTTPASFEIVPRALRVRVPRH"
     gene            complement(7453..7749)
                     /locus_tag="VF225_00080"
     CDS             complement(7453..7749)
                     /locus_tag="VF225_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143680.1"
                     /translation="MPRPRQKNIVERLAGAGEEAIQRIGSAPGADKVVGALGTLRDRV
                     DELQKSVRAIDRLAKRLTAIEKRLDKLEGKGTASSRKTSSAKRSTAARKRTTGS"
     gene            7802..>8751
                     /locus_tag="VF225_00085"
     CDS             7802..>8751
                     /locus_tag="VF225_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7143681.1"
                     /translation="MKGAAALLALLAFVAASAGEAKPTPVTIRVLTRNLYLGTNLDAI
                     VQAKSTSEAFSAVEKGWANVQANDFPTRARAIAKEIASAKPDFVGFQELSLYRTQTPA
                     DFTVTPATTVVLDYAKELRNALAARKLHYRFLAIGNETDAELPSGDPPNMDIRLTIRD
                     GLLVRIDKKIKIRRVRTGLYSTTTPLFGGFVIAKRGWVLADATVGGRTFRVVTTHLES
                     FNDTSQVAQGKELVALGGPAATSLPTILLGDLNSRADGTSTPTHANLLAAGFQDAWPQ
                     VHPGEIGLTCCHGDDLHELGGPFYSRIDYVLMRNGFRAVGA"
BASE COUNT         1431 a         2978 c         2886 g         1456 t
ORIGIN      
        1 atcgacacga gcagccgcaa ccgcagcggc cggaccggat cggcctctgc gtcggcgtcg
       61 gacggaagcg ccgccgtgta accagcttct tccacagtgc gcaccagatc gggcaggtgc
      121 acgtgacccg ggtcgtacgc aacagatgcc agctcagtcc cgtagttgac gctcgcggag
      181 accccttcga gctggttgag ctccttctcg acccgtgctg cacaggaggc gcaggacatg
      241 ccaccgatct cgagctcgag gcggctggga gtttcgaccg aggtcatcga gccatgatac
      301 cccgaggggg taccaggccc gggctagcct ttagcgcctt cgggcggcgg aggctgcatg
      361 gtcgccccgg ggatcgggaa cggccgggtc ttcggctcgg gcttccgctt cttctcggct
      421 tcgggctcgg gctccgtctt cggcgccggg gtcttctccg caaagacggc ttcctcgtcc
      481 tcgccggcga gcaggcgctc gaactgatcc ttgtcgatcg tctcgcgctc gatgaggatc
      541 agcgagatcc ggtgcagctc gtcgctgtgc tccttgagca cctcggttgc cgtcatgtgg
      601 gcttcctcga tcacgcggcg gatctcgtcg tcgatctcgc gcgcgatctc ctcggagtag
      661 tcgggctcgt tgcccatctc gcggccgagg aacggcatgt cgtggttacg acccagcacg
      721 cgggggccga gcttctcgct catcccgaag cgcatgatca tctgcttcgc ggtgtgcgtg
      781 accttctcga ggtcattcgc tgcgcccgta gtgacctcgt tgaagacgat ctcctctgcg
      841 gcgcggccgc cgagcgtcat cgccaactgg ttcatcaaag tctgcttcgt cgtcagatac
      901 cggtcctcgc gcggcagcga gatcgtgtac ccgagcgcct gcccgcggcc gatgatcgag
      961 atcttgtgga cgtcggattc cccgtcgagg tagtggccga cgatcgcgtg gccgagctcg
     1021 tggtaggcgg tgatcttccg ctcctcctcg gagaagagac gcgtcttctt ctccgggccg
     1081 gcgatcacac gcatgatccc ttcctcgagc tcctcctgct cgatcacttt cttgccgcgg
     1141 cgcgccgcga gcagcgccgc ctcgttgacg aggtttgcca ggtcggcgcc tgtgaagccc
     1201 ggcgtgccgg cagccagcgt gtcgagatcg atctccgacg cgagcggctt tcctttggtg
     1261 tgcaccgaca ggatcttgct gcgcccgttg cggtcgggac ggtcgacgac gatctgcctg
     1321 tcgaagcgac caggtcgcag cagcgccgga tcgagtatgt ccggcctgtt ggtggccgcg
     1381 atcaaaatga tgttgtcctt cagctcgaac ccgtccatct ccacgagcag ctggttcaag
     1441 gtctgctcgc gctcgtcgtg gccgccgccg agacccgcgc cgcgatggcg gccgaccgcg
     1501 tcgatctcgt ccatgaacac gatgcacggc gaagcctgct tggcctgctc gaagaggtca
     1561 cgcacgcgcg aggcgccgac accgacgaac atctcgacga agtcggatcc cgagatggag
     1621 aagaacggca cgccggcctc gcccgcgact gcgcgcgcca gcagcgtctt gccggtaccg
     1681 ggcggcccgt acagcaacac acccttgggg atgcgcgcgc cgagcgcctg gaacttcttc
     1741 gggttctcga ggaactcctt gatctcctgc agctcctcga ccgcctcctc gaccccggcg
     1801 acgtctttga acccgatctt cggcgagtcc ggagtcatcc gcttcgcacg cgactttccg
     1861 aacgacatca ccttcgagcc gccgccctgc acctggttca tcaggaagat ccagaagccg
     1921 aacagcaaga cgaacggcag cagcgacgtg aggatcgacc accaggggga accgcccgtg
     1981 cccttcgagt cgaaattcac gttgtgctgg gccatcagtt tctggaggtc ggcggccgac
     2041 tggtcgctcg gatagtggac ggagcccttc gtcttgtcgg tgagcgtgta gttcagcgct
     2101 tgcttgttcg ggttgaagac gatctgcgcg aagtcacacc cactcccggc gaccgtgttg
     2161 caggcctcga tcttctgctg gacctgcgag agcgtcagct tcgaggtggg ctgactgtgg
     2221 ccgcccagcg tctggagggc cagccaaacg agcgccgcga tgatgacgag ggggaagagc
     2281 gcgctgcgga agaatctatt cactagacct ctttcggcag ggtaccagcc taggtttgag
     2341 ctacagcagc cccaaacggg ggtgatccgg gctaattcag gcgccctcgg gcaccaactc
     2401 cgaatccaat acgcccacat agggcaggtt cctgtatcgc tcggcgaagt ccagcccgta
     2461 gccgacgacg aaacgattcg ggatctcgaa gccgatgtag cggacgggaa cgtcgatttc
     2521 ccgccggtcc ggcttggtca ggagggcgca gacctcgagg gtggcgggct cgcgggcctc
     2581 gaggttccgc atcaggtacg agagggtcag ccccgagtcg atgatgtcct cgacgacgag
     2641 gacgttgcgg ccctcgatgt tgatgtccag gtccttgagg atccggacta cccccgacga
     2701 gtcggtctgg gccccgtagc tcgagatcgc catgaagtcg atctcgcagg ggatcgcgag
     2761 cgtccgcatc aggtcggcca tgaagaagac ggcgcccttg aggacgccga tcagcagcag
     2821 gtcccggccg gcgtaatcgg ccgagatctc ctccccgagc tcgaccaccc gcgaccgcag
     2881 acggtcctca tctatgagga cagccgaaac gccgcgctcg agctcagtct gggacacgtt
     2941 cgacgcgcac tccttccgcc tcgacgatgc ccggcaccgc gaccacgtcg tctccacgca
     3001 cgacaagcgg ccacgactcg cgtgcggaac gggggatctt cgcgtcgacg aacacgtcct
     3061 gaatcttctt cgatctccca gcgagccgat cgccggcgcg ccaggctcgt accttaaggc
     3121 ctgggagcct cgaggcgatc cgccattcgc cccagcgcac ctcgccgtcg agtgcgaccg
     3181 gcgtgcgctc gagccagacg cggtcgtact cgcgcacggc ggtcagaccg gcgccgagat
     3241 cgagccgcct cgacccggct gtcgacgcaa gcagctcgtc cagcgctgtc ggctcccctt
     3301 ccgcgaggcg gagcagattg ctctcggcgg cgggatgcag ctcgcgcaga cgcgggagga
     3361 tctcgtcgcg gatcaggccg cgcttcgtgt cgctgttgga ctcgtcggtc cggaactcga
     3421 gcccttcggc acggcagtac tcctccgtct cagcgcgcca gacggagagc agcggacgga
     3481 cgacgccgtc ctcgcgacgc accttgatcc ccttcgccac cccgctggcg acgagcttgt
     3541 acaggaccgt ctcgacctga tccgaggcgg tgtggccggt ggcccggaga cggtcggtcg
     3601 cgaacgagta ccggctcctg cgccaatcgt cctcggtcag cccgccgctg ccgtcgacca
     3661 cctccgcgcc gaagcgctcg gcgcagaagc gcgcatcctc gtccgaggct gcgccccgca
     3721 aaccgtggtt cacgtgcaga gccgaaacgc gatagccgag ctccgtcagg acgtgccaga
     3781 ggcaggtcga gtcggcgccg ccggagacga ggcaggtcac gtccccagcc ggcgcgatca
     3841 gatcgtgcct gcggaccagc tcctcgaccc gttcgcgcag cctctccgtg tccacgagag
     3901 cagcgtagac aggcgaggga ttgccggcca gggtccgtcg gccgatgttc cggacatggc
     3961 cgagttcttt caggcctggc ggaagcgcca gtccgagaag caggctgcgg aatggaccga
     4021 agagcaggag gaagcccgcc gcgcggtcca agagcttccc gacgtcctgc gagacagagc
     4081 tcgcctcgcc gtcgagaagc tgatcgacgg ccccgatgcc gaggtcgaag aagcgctcga
     4141 ggagctcgac cacctgctcg agccgcaccc cgatctgaag gaccgcatct tccggctccg
     4201 cgttgtcgac gacgcggtgg aattcctgaa ataactagag caggggtgag acgtcgcagc
     4261 cgcgcttggc gagtgcgggc ttcgcctggg acaggatgtc gtgcgcgaaa ttcgccagcc
     4321 cgtaggcccg gtagatgtac ttgcccggat cgtggtgatg cttgtcggcg cggatggccc
     4381 gaccgtactc caggaacatc gcgcgtagga tcttctgcgt cttcgaaagt gacggatcgc
     4441 tcggattcgt gttgagcagg ctcgtgaccg cggagtccgt ctgcaagatc acgtcgtcgg
     4501 ccttggcgtc gccctggagg tagtcctgcg agagcgtgcc gagcgccgcc atgttcagct
     4561 ccgcggcacc gaggaactgc cggtcggtcg ccgagcaggt gtggctgaca gcagccttgc
     4621 tggttcccgt gttgtcgagg acgacgaaag aggccacgac gaagatcgcc atggcaagtc
     4681 ccgcggccgc agtgatgcgg ggcttcacgc tccctacttc ggcgtcgaag caggggaggc
     4741 ttcatcaccc gtttgggtgc gtgaccgatg ccttgtgaca taggacgcaa actgctgggc
     4801 gacccaccgc aggatccaca gggcgaaggc gagccgcagc aggcggatca aaagatcagc
     4861 gaccggtagg aaccgtgccg ctgcattcca atcgcttcgt agacgcggat cgccggtgcg
     4921 ttctccggtc ggacgaaaag gcagacggtc ggtacgcgct cgagcagcag gcggcagaga
     4981 tcccgcatcc cgcgctgcgc gtagcccttg cggcgcgcag gcggatccac ccagacctgt
     5041 tgcacctgca ccgcctccgg tgtccaggcc gaggcctccg ccttgaagag gatcgtcccg
     5101 cgctcgaccc aaagccacga gcgtccctcc tcgatctgcg agcgcgtgcg ccagcggaag
     5161 ccctcgggat cgcgggccat cggatcgact ccaatctcct cctcgtgcgc cgcagcgctc
     5221 gcgggcagca gcaacgggaa gtccttgtcc gtggccggcc ggagcccggt gtcgccggca
     5281 gtcggcggtt ccgagatcgc gtacaccggt tgcccgggcc ggtcgtttcg cggcgccggc
     5341 atcagcctcg cgagatcgtc ccagagcgcg tcgaccgcgt gctcgtcgcc gatgatcatg
     5401 cgcgcgcggc cgcgagaggc ggcttgcgcg aaacggccgc actccgagcc ggagggaacg
     5461 acgttggcgc cgacgtggca gagagcggtc aagcggccgt tgtcggcgaa ggcgctgaac
     5521 cgtcccagcc gacgccgggc aacgtcctcg aggaagacgc gctccacggg gtcctcggcg
     5581 cagaactcga ggatctgctc gagccggggc tcgatcgcac ctttcactgc cccaatagtc
     5641 gcacgggtta gggttgcgcc ctcatgccga tccacattcg agccgagccg ggcgagtacg
     5701 ccgacgcctg cctcctcccc ggcgatccgc tgcgggcgaa gtacatcgcg gagacctact
     5761 ttgacaatcc ggtcgagaag aactccgagc gcggtctgct cgggtacacc ggcacctacg
     5821 atggcaagcc ggtctcggtg cagggcaccg gcatggggtg tccgggcgcg acgatcgtct
     5881 tcgaggagct gatccagctc ggcgtgaaga agctgctgcg cgtcgggacg tgcggaggcc
     5941 tccaggccca ccatgagctc ggtgacctga tcgtggcctt gtctgccgtt gccgacgacc
     6001 gcaccgccga tcacctcgtc gggcacgagc cgcactgtcc gaccgcgagc tgggagctca
     6061 tccacggcgc cgtgcacacg gccaagcaga tgggccagtc gatgcatgtc ggcccgatcg
     6121 tctcgagcga cgtcttctac aaccccgacg agaaccagta cgaacggtgg tcgaagcgcg
     6181 gcgtgctggc ggtggagatg gaggcctccg cgctcttcac agtcggtgct ctccgcggcg
     6241 tccagtcggg gtgtctcctc accgtgagcg acatcgtcgt cgagggtgag ttcaagcgca
     6301 tcaccgacga ggagctgcgg gcggctgtcg accggatgac gcgcgtcgcg ctcgcgacgg
     6361 tcacggccga cgagaaacac tgaccaagac cgtctttctg atcaaccccg cgtcggcgaa
     6421 cggctcgacc ggtaggcgct ggcctgagat cgcccggcgt gcggccgcgg cagggctgac
     6481 cggagatgcg ctcttctcgc gctcggccgg cgagctgacg gagctggcgg gccgcgccgc
     6541 ggacgaaggc gccgagctgc tcgtcgtcgt cggaggcgac ggatcggtgc acgaggtggc
     6601 gaacgggatc gccggacgca gcgaggtcga gatcgcggtg atcgcgcgcg gaaccggctg
     6661 ggatttcgcc cgctcgctcg gcctcccgcg caacatcgac cgcgccatcg acgtcgccct
     6721 ctccggcgag acccggacga tcgacctggg ccgcgcgacc taccggctct gggccggcgg
     6781 tgaaggacag tccttcttcg cgaacgtcgc gagcgcaggg atgagcggcg cgatcgccca
     6841 gcgtgccaac gacagctcca aggcactcgg cggcaaggtc tcgtacgtct gggcgacgtt
     6901 cgccgtcttc gcgcgctggt cgagcagcga gatcgaggtc tccgtggacg gcgagactcg
     6961 ccgagccctg atgcacgacg tcatcgtttc caacggcccc tacgtcggcg gcggcatgaa
     7021 gatctgtcct gatgccgagc tcgacgacgg catcttcgac gtgctgctca tcggcgacct
     7081 gacgaaacgg gatctagtgc tgacgatgcc gaagatctac cgcggaacgc atcttcccca
     7141 cccgaaggcc gagctcctgc gcggctctca ggtaacagtc gacgcagccg atcccctccc
     7201 gatcgagctc gacggcgagc agccgggaac aacgcccgcg agcttcgaga tcgtcccgcg
     7261 ggcgctgcga gtccgcgtcc cgcggcacta gtcccgtgac acacgcgtca ccgtttcgta
     7321 cccgtgcctg gcaccggtac gaccgcgaga acgccacgcc tgctcagggc cgtgcttgtt
     7381 ctgtgacaca cgcgtcagcg tttcgtaccc gtgcctggca ccggtacgag gccgaaagcg
     7441 acgtgccctt ctctagctgc cggtggtgcg cttgcgcgca gcagtggagc gcttggccga
     7501 ggaggtcttc cgcgagctcg ccgttccctt gccctcgagc ttgtcgagcc gcttctcgat
     7561 cgcggtcagc cgcttcgcga gccggtcgat ggcacggacg ctcttctgca actcgtccac
     7621 gcggtcccgg agcgtcccga gcgcgccgac caccttgtcc gccccgggtg cgctgccgat
     7681 ccgctggata gcctcctcgc ccgctccggc gagccgttcc acgatgttct tctgtcttgg
     7741 cctaggcatg gccctatcgt gccagttgcg atggccgccc gtcgtgccta cgctcattcg
     7801 cgtgaaagga gccgccgcct tgctcgcgct cctcgccttt gtcgccgcct cggccggaga
     7861 ggccaagccc acgcccgtca cgatccgtgt gctgacgcgg aacctctatc tcgggacgaa
     7921 cctcgacgcg atcgtgcagg cgaagtcgac cagcgaggcc ttctccgcgg tcgaaaaggg
     7981 ctgggccaac gtccaggcca acgacttccc gacccgcgca cgcgcgatcg ccaaggagat
     8041 cgcgagtgca aagccggact tcgtgggctt ccaggagctg tccctgtacc ggacgcagac
     8101 gcccgccgac ttcacggtga cgccggcgac aaccgtcgtg ctggactacg cgaaggagct
     8161 gcgcaacgcg ttggcagccc ggaagctgca ctatcgcttc ctcgcgatcg gcaacgaaac
     8221 ggatgcggag ctgccgagcg gcgatccgcc gaacatggac atccggctta cgatccgaga
     8281 cggcctgctc gtgcggatcg acaagaagat caagatcagg cgcgtccgga cgggtctcta
     8341 ctcgaccacg acacccctct tcggaggctt cgtcatcgcg aagcgcggct gggtgctcgc
     8401 ggacgcgacc gtcggcgggc ggaccttccg ggtggtcacg acgcatctcg agtcgttcaa
     8461 cgacacatcc caggtcgcgc aggggaagga gctcgttgct ctcggcggcc cagcggcaac
     8521 aagcctccca accatcctgc tcggcgacct caactcgcgg gccgacggga cgagcacccc
     8581 gactcacgcg aacctactcg cggcggggtt ccaggacgcg tggccgcagg tgcaccccgg
     8641 cgagatcggg ctcacgtgct gccacggcga cgatctgcac gagctcggcg gcccgttcta
     8701 cagccgcatc gactacgtcc tgatgcgaaa cggtttcagg gcggtgggtg c
//
LOCUS       DASVEU010000004         6036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_196841,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000004 DASVEU010000000
VERSION     DASVEU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196841"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..563)
                     /locus_tag="VF225_00090"
     CDS             complement(108..563)
                     /locus_tag="VF225_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7143682.1"
                     /translation="MRLTVNGEQRDLHSPPLASLLTALREELDITSPKAGCHQGGCGA
                     CTVLVDGEARRSCLLPLAAVDGATITTVEGLGAADDLSPVQAAFDEHYAAQCGFCTSG
                     FIMAATALIDRTPKANREEILAGLSGHVCRCTGYIKIVSAVETAAGSPA"
     gene            complement(560..1366)
                     /locus_tag="VF225_00095"
     CDS             complement(560..1366)
                     /locus_tag="VF225_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7143683.1"
                     /translation="MAATSLAEAQVIAPTSRAEAVEAFGDGSGVTVLGGGTILMPEMN
                     YGRLRPERVLLLQNAGLSGIARNGTTLTIGAMTTVAELEQAPEPLGTMARNVADHEIR
                     GQATLGGNLCVHAGGATPRGDLQAALIALGAQVKTAGKDGERTEAIEDFVAAPDGRLL
                     LEVEFEEPKRSGHAAAHRPHAHAYTILAVCAAETPAGLRVGLSGAGRHGLRARSVEDA
                     LAGGASTAEAAQKALDDAQTSDDALASAWYRQRLLPILVQRALDDLGGGK"
     gene            1433..2323
                     /locus_tag="VF225_00100"
     CDS             1433..2323
                     /locus_tag="VF225_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7143684.1"
                     /translation="MVFKTIDELEAGLRSARYLPERGLATALYLSLALEKPLLLEGEA
                     GVGKTEAAKALAEATSSRLIRLQCYEGLDVAHAVYEWNYSRQLLHIRAAQEGTVSEQE
                     LFGPEFLIRRPLLEAIESKEPVVLLIDEIDRADDEFEAFLLEMLSDFQITIPEIGTVS
                     SSRRPAVILTSNRTRELHDALKRRCLFHWIDHPTLEREIEIVVLRVPGIPDRLAGQAA
                     AFVQGLRQLDLAKPPGVAETIDWAQALAALGREELDAGVVEETLGSVLKYHEDFAAVR
                     DEALVALVEEARSVAAAGAV"
     gene            2298..3446
                     /locus_tag="VF225_00105"
     CDS             2298..3446
                     /locus_tag="VF225_00105"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7143685.1"
                     /translation="MSPQPAPSEGGFVRHVVTFGRILREAGLEVGPGRISDALTGLDC
                     LDIRNRDDVYWTLRTTLVSRAEELESFDRAFAAWFLRSPSRPPYRGTLPIDKPPRLSE
                     RTRTDRTGTDGRGGDEVTEIGWSRHEVLRRKDFAAMSPEEFAQLSKAIAEVAASRPLR
                     RSRRLRPHSRGRTLDLRRLARRSLARGGDPIERTFRRRIEVPRKLVLLCDVSGSMEAY
                     TRALLLFMHAAVGSGRGVEVFVFGTRLFRLTDEFRTRDPEEALAAAARRVVDWAAGTR
                     IGASLKTFNDTWGRRALTRGAAVVIVSDGWEREDPALVAREMARLQRAAYAIVWVNPL
                     KGNPEYQPLAEGMRAALPFVDRFVAGHNLASLEELAGVLAGIERRHAA"
     gene            3443..4522
                     /locus_tag="VF225_00110"
     CDS             3443..4522
                     /locus_tag="VF225_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7143686.1"
                     /translation="MKEVLPDIERWRARGERFAVATVIATRRSAPRPVGAKFAVSASG
                     EMAGSVSGGCVESDVYDHACQVLEGARPELLSYGIADELAFSVGLPCGGEIDVFVENA
                     PDALVERLLPIIESEERAILFTVVEGEPLGADLLVTEAGDRFGDGPEELAGHVDELLR
                     RGRNTLLELDDGRKVFAEVYGPAPRLLVIGAVDTAESLCAAAKQLGWRTIVADARGKF
                     ATKERIPSADELLVSWPQEAIDQVQPDYQTAVVVLTHDDKFDVPALKGALGTEAFYIG
                     ALGSRRNQERRRERLLEAGVEEHQFERVSGPTGLDIGADTPAETALSILGEILATRAG
                     RDGGFLRNSKTRIHVEETADALARD"
     gene            4788..5702
                     /locus_tag="VF225_00115"
     CDS             4788..5702
                     /locus_tag="VF225_00115"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7143687.1"
                     /translation="MAVDVKSEKLHELVDANAQVEKLATGFTFTEGPIWNKEGRFLLF
                     SDMPADIRRRWSEADGVTEVANPSNKGNGMTYDSEGRLLVCEHVTSSLVRMDPDGQGG
                     NREILASHYEGRELNSPNDVIVANDGSIYFSDPWYGRMPVFGIERDRELDFQGVYRIA
                     PEGGDPQLLVDDFEQSNGLCFSPDESLLYVNDTPRAHIRVFDRQPDGTIANGRMFFEN
                     IGTGVIADGIPDGMKCDEHGNVYVTGPGGVWVISPDAELLGIIEVPENVGNLNWGGDD
                     WSDLYMPSTTSLYRISMKVSGNRLSYMT"
     gene            5713..>6036
                     /locus_tag="VF225_00120"
     CDS             5713..>6036
                     /locus_tag="VF225_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX7143688.1"
                     /translation="MSETERIDVDPGRAALIIQDLQNDVMADGGAFAESGAPQHAKEQ
                     NVVENVKQLAEACRAVGIPVIHVHYIVEEGAPGLKLNAPLFQGVKEGNALVRGTWGAA
                     AVDGLE"
BASE COUNT          989 a         1970 c         2096 g          981 t
ORIGIN      
        1 cgccttcgtc cagagggtgc ctcgaacgcg gatgtcgtcg acgtactggg tctgtccggt
       61 gacgtgggcg acgccgtcgt agcgcggcag tcgcgcgccg acggccttca cgccgggctc
      121 cccgctgccg tctcgaccgc gctgacgatc ttgatgtacc cggtgcaacg gcagacgtgc
      181 cccgacagcc ccgccaggat ctcttcgcgg ttcgccttcg gcgtccggtc gatgagcgcg
      241 gtggccgcca tgatgaagcc ggatgtgcag aagccgcact gtgcggcgta gtgctcgtcg
      301 aacgcggcct gcacgggaga gaggtcgtcc gcagcgccca ggccctccac cgtcgtgatc
      361 gtggcaccgt cgactgcggc gagtggcagc aggcatgacc gtcgcgcctc gccgtcgacg
      421 aggaccgtgc aggcgccgca gccgccctgg tggcagccgg ccttggggct cgtgatgtcg
      481 agctcctcgc gaagcgcggt gaggagcgag gcgagcggcg gactgtgcag atcgcgctgc
      541 tcgccgttga ccgtaagtct catttccctc ctccaaggtc gtcgagtgct cgctgaacca
      601 agatggggag cagccgctgc cggtaccagg ccgacgcgag cgcgtcgtcg gaagtctggg
      661 cgtcgtcgag cgccttctgg gccgcctctg cggttgaagc tcctcctgcc agcgcgtcct
      721 cgaccgagcg cgcgcgcagg ccgtgtcgtc cggctccgct caggccgaca cgcaacccgg
      781 caggcgtctc ggccgcgcag acggcgagaa tcgtgtacgc atgtgcatgt ggtctgtgcg
      841 ccgctgcatg gcccgagcgt ttcggctctt cgaactcgac ttcgagcagc agccgtccgt
      901 cgggggcggc cacgaagtcc tcgatcgcct cggtgcgctc accgtctttg ccagccgtct
      961 tgacctgggc gccgagagcg atcagcgcag cctgcaggtc gccgcgcggg gttgcgccgc
     1021 cggcgtgcac gcacagattt cctccgagcg tcgcctggcc gcgaatctcg tgatctgcaa
     1081 cgttgcgtgc catggttccg agcggctcgg gcgcctgctc gagctcggcc accgtcgtca
     1141 tggctccgat tgtcagagtc gtgccgttgc gtgcgattcc cgacagcccg gcgttctgca
     1201 gcagcaggac acgttccggt cggagccgtc cgtagttcat ctcgggcatc aggatcgtgc
     1261 cgccgccgag cacggttacc cccgagccgt ccccgaaggc ctcgaccgcc tctgcccgcg
     1321 aggtgggggc gatgacctgg gcctctgcaa gagatgtcgc tgccactccc cgctccctcc
     1381 tcgaaggcgc caaccgagcc gattctgtca cagcccggtt atcgtgagag aggtggtgtt
     1441 caagacaatc gatgagctcg aggccggcct acgaagtgcg aggtacctgc cggagcgtgg
     1501 tctggcgacc gctttgtacc tctcgctcgc gctcgagaaa ccgttgctgc tcgaagggga
     1561 ggccggggtc ggcaagaccg aggcggccaa ggcgctcgcg gaagccacct cttcgcgtct
     1621 gattcggctg cagtgctacg aaggcctcga cgtcgcccac gcggtctacg agtggaatta
     1681 ctcacgccag ctccttcaca tccgagcggc tcaggaaggc acggtgtcgg agcaggagct
     1741 cttcggcccc gagttcctga tccggaggcc gctgctcgaa gcgatcgaga gcaaggagcc
     1801 ggtcgtgctg ctgatcgacg agatcgatcg ggcggacgac gagttcgaag ccttcctgct
     1861 cgagatgctc tcggacttcc agatcacgat tccggagatc gggactgtca gctcgagccg
     1921 ccggccggcc gtgatcctga cctccaatcg cactcgtgag ctccacgacg cgctcaagcg
     1981 gcgctgcctc ttccactgga tcgatcaccc gacactcgag cgtgagatcg agatcgtcgt
     2041 gttgcgcgtg cccggcatcc ccgaccggct ggccgggcag gcggcggctt tcgtgcaagg
     2101 tctgcgacag ctcgatctcg cgaagccgcc gggtgtggcc gagacgatcg actgggcgca
     2161 ggctcttgca gcactcgggc gtgaggagct cgacgcggga gtcgtcgaag agacgctcgg
     2221 ctccgtcctg aagtaccacg aggatttcgc cgcggtccgc gacgaggctc tcgtcgcgct
     2281 tgtcgaggag gcacgcagtg tcgccgcagc cggcgccgtc tgaaggcggg ttcgtccgcc
     2341 acgtcgtcac gttcgggcgc atcctgcgtg aggcgggatt ggaggtcggc ccggggcgga
     2401 tctcggacgc gctcactgga ctcgactgcc tcgacatccg caaccgcgac gacgtctact
     2461 ggacgttgcg cacgacgctc gtgtcgcgag ccgaggagct cgagagtttc gatcgagcgt
     2521 tcgccgcctg gttcctccgt tcgccctcca ggccgcccta ccgcggcacg ctgccgatcg
     2581 acaagccgcc gcgtctgtcg gagcggacgc gcaccgatcg caccggcacc gacggccgcg
     2641 gcggcgacga agtcaccgag atcggctgga gccggcacga ggtcttgcgg cggaaggact
     2701 ttgccgcgat gtcgccggag gaattcgcgc aactctcgaa agcgatcgcc gaagtggcag
     2761 cgtcgcgacc gcttcgacga tcgagacgct tgcgcccgca ctcgcgcggc cggaccctcg
     2821 acctgcgacg ccttgcgcgc cgttcgctcg cccgcggcgg cgatccgatc gagcgcacgt
     2881 tccgccgccg catcgaggtg ccgcgcaagc tcgtcctgct ctgcgacgtc tcgggctcga
     2941 tggaggccta cacgcgagcg ctgctgctct tcatgcatgc agcggtcgga tccgggcgcg
     3001 gcgtcgaggt gttcgtcttc ggcacgcgcc tgttccggtt gacggacgag ttcagaactc
     3061 gtgatcccga ggaggcgctg gcggcggcgg cccggcgagt cgtcgattgg gcggccggta
     3121 cccggatcgg tgcatcccta aagacgttca acgacacctg gggacggcgc gcactcacac
     3181 gcggtgcagc tgtcgtgatc gtgtcggacg gctgggagcg cgaggatccg gcgctcgtcg
     3241 cgcgggagat ggcacgcctc caacgtgcgg cgtacgcgat cgtctgggtg aatccgctga
     3301 agggaaatcc ggagtatcag ccgcttgccg aagggatgag agcggccctt ccgttcgttg
     3361 atcggttcgt ggccggacat aatctggcga gtctcgaaga actggccggc gtccttgccg
     3421 gtatcgaaag gaggcacgcg gcgtgaaaga ggtgctgccg gacatcgagc gctggcgcgc
     3481 acggggcgag cgattcgccg tggcgaccgt gatcgcgacg agacgctccg cgccgcggcc
     3541 ggtcggggcc aagtttgctg tctccgcgag cggtgagatg gccggctcgg tctcgggagg
     3601 ctgcgtcgag agcgacgtct acgaccacgc gtgccaggtg ctcgaaggag cgcgcccgga
     3661 gttgctctcg tacgggatcg cggacgagct cgcgttctcg gtcggtcttc cctgtggcgg
     3721 cgagatcgac gtcttcgtcg agaacgcgcc ggacgcgctg gtcgaacgcc tcctgccgat
     3781 catcgagagc gaggagcggg cgattctctt caccgtcgtc gagggggagc cgctcggcgc
     3841 cgacctgctc gtgaccgaag ccggtgaccg gttcggcgac gggcccgagg agctcgccgg
     3901 ccacgtcgac gagctgctgc ggcgaggacg caatacgttg ctggagctcg acgacggccg
     3961 gaaggtgttc gccgaggtct atgggcctgc tccgcgtctg ctcgtgatcg gggccgtcga
     4021 tacggcggaa tcactttgcg ctgcggcgaa gcaactgggc tggcggacga tcgtcgccga
     4081 cgctcgcggc aagttcgcga cgaaggagcg catccctagc gcggacgagt tgctcgtctc
     4141 ctggccgcag gaggcgatcg accaggtgca gccggactac cagaccgcgg tcgtggtgct
     4201 gacgcacgac gacaagttcg acgtccctgc gctcaagggc gcgctcggga ccgaggcgtt
     4261 ctacatcggc gccctcggct cgcggcgaaa tcaggagcgc cggcgcgagc gactgctcga
     4321 ggccggcgtc gaggagcacc aattcgagcg agtctccggt ccgacggggc tcgacatcgg
     4381 tgccgacact ccggctgaaa cggctctctc gatcctcggc gagatcctcg cgactcgcgc
     4441 cgggcgcgac ggaggcttcc tgcggaactc gaagacgcgc atccatgtgg aggaaaccgc
     4501 ggacgcgctc gcccgcgact agacgagcgc cgcgcgggga cacatccgtc ccaaagacga
     4561 cacgctctcg tcacgtggcg agcggtacgg tgagagagcc tcactagtcc aacctcagcg
     4621 gacacgtcac atgccgacat gtcttggggt ctgaccccaa gacagtgcct cgaaaggaca
     4681 gggcgactcg tcctctgcag tcgcgtgcaa cgtgcccgcg cttccgccca gagccgcgaa
     4741 gtgttaggtt cggccggctt ttagattgcg cgacaaaagg agccgagatg gccgttgacg
     4801 tcaagtcgga gaagctccac gagctggtcg acgcgaacgc gcaggtcgag aagctcgcga
     4861 cgggcttcac gttcaccgag ggaccgatct ggaacaagga agggcgcttc ctgctcttca
     4921 gcgacatgcc cgccgacatt cgccggcgct ggagcgaagc agacggcgtc accgaggtgg
     4981 cgaacccgag caacaagggc aacgggatga cctacgactc cgagggccgc ctgctcgtgt
     5041 gcgaacacgt cacgagctca ctggtgcgca tggatcccga cggtcagggc ggcaatcgcg
     5101 agattctcgc ctcgcattac gagggcaggg agctgaacag cccgaacgac gtgatcgtcg
     5161 caaacgacgg ttccatctac ttcagcgacc cctggtacgg acgcatgccg gtcttcggga
     5221 tcgagcgcga ccgcgagctc gacttccagg gcgtctaccg gatcgcgccc gaaggcggcg
     5281 atcctcagct tctggtcgac gacttcgaac agtcgaacgg gctctgcttc tcgcccgacg
     5341 agtcgttgct gtacgtcaac gacacgcccc gagcgcatat ccgcgtcttc gatcggcaac
     5401 cagacgggac aatcgcaaac ggacggatgt tcttcgagaa catcggcacg ggtgtgatcg
     5461 cggacggaat ccccgacggg atgaagtgcg acgagcacgg caacgtctac gtcacggggc
     5521 ccggcggcgt ctgggtgatc agtccggacg cagagttgct gggcatcatc gaggtccccg
     5581 agaacgtcgg caatctgaac tggggcggcg acgactggag cgacctgtac atgcccagca
     5641 cgacctccct gtaccggatc tcgatgaaag tgtcaggcaa tcggctcagc tacatgacgt
     5701 gaggaggaga cggtgagcga aaccgaacgc atcgacgtcg atccagggcg cgcggccctg
     5761 atcatccagg acctccagaa cgacgtgatg gcggacggcg gtgcgttcgc cgagtccggc
     5821 gcgccgcagc acgcgaagga gcagaacgtc gtcgagaacg tgaagcagct cgcggaagcg
     5881 tgccgggcgg tcggcatccc ggtgatccac gtgcactaca tcgtcgagga gggtgcgccc
     5941 ggcttgaagc tgaacgcacc gctcttccag ggcgtgaagg aaggcaacgc gctcgtgcgg
     6001 ggaacgtggg gcgcagcggc ggtggacgga ctcgag
//
LOCUS       DASVEU010000005         5539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_973878,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000005 DASVEU010000000
VERSION     DASVEU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973878"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VF225_00125"
     CDS             complement(<1..1066)
                     /locus_tag="VF225_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX7143689.1"
                     /translation="MKRKLALATAATLALAAGLAVGAREHGSSALAAPPAQRPNVLIL
                     ETDDQTLAEMEVLPNVRRLIGDQGVTFDNNFDSFSLCCPSRATFLTGQYSHNNGVRGN
                     APPQGGYQALDKSNTLAVWLQRGGYYTVHLGKFLNGYGRQSPTEIPPGWSEWHGAVDP
                     TTYRYYNYTLNEGGTLHTFCATPQPSCYQTDVYRDKANEIIRRRAAQGPFFLWVAFLG
                     DHSGAPREPDDPPQLGTPVPAPRHHDALKGTPLPRPANFNEADVSDKPRVIRQRPLLN
                     ARRIAAIQENWQQRRETLMSVDEAVASIVETLRQAGELDNTLILFTSDNGFFHGEHRV
                     ANGKVLWYEPSIHLPLLMRWT"
     gene            complement(1150..2418)
                     /locus_tag="VF225_00130"
     CDS             complement(1150..2418)
                     /locus_tag="VF225_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7143690.1"
                     /translation="MTAAILRAGARTFLSVRKHRNYRLFFTGQVISNIGTWMQRVAQA
                     WLVLSLTHSPFAVGILALCQFMPFTLFSLIAGVVVDRLNAWRTVIGTQLMQMVLASTI
                     AVIALAGVARPWHVYVIAALMGLVQVLDAPSRQQLTFRMVGPLELPNAISLNSGVFNG
                     ARIFGPALGGVIIAAAGAGVCFAVNAASYIAVLAGLLMMRPQEFHAVERRARPRTLYV
                     GLKEGISFARNHEQIRLMLMLTFVVSTFCLNFNVLLPVLAKHTLHSGPQVFGLLSAVF
                     GVGALIGALVSAHVSRATVGTTAVGSAGFALCELLISPLRSIALIALLLFLGGVFFTT
                     WSSNSSSLIQLAAPDHLRGRLIGIYFFAFAGTGTLGGIMSGWLTALGGTELSFAVAGV
                     AGLSMSLYVWLRSRTVPLVEERPAEELIAA"
     gene            complement(2415..2861)
                     /locus_tag="VF225_00135"
     CDS             complement(2415..2861)
                     /locus_tag="VF225_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7143691.1"
                     /translation="MPIAVAADPTILANRLRPVLLQLNRQLRREIHSLGVTGGQVSLL
                     VQIKYHPGIGIRELAALERMSVPGMSKFISRLEEAGLVQRAPVEGDQRRVGLTLTAQG
                     QKVLRSVKSKRTAWLAARLRELGADELERIDAAIEPLAHLLAEDEA"
     gene            2941..3957
                     /locus_tag="VF225_00140"
     CDS             2941..3957
                     /locus_tag="VF225_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7143692.1"
                     /translation="MARNPLEDVARGLRVLGERLSQTTGAPDPSDWISDQLRRPEPVR
                     TLPELQAELDSLIGLETVKEQVRALVAFLQVQAERQRHGLPEVATSQHLVFLGNPGTG
                     KTTVARLLAEMYRAMGLLKRGHLVEVDRAGLVGQFVGMTAIKTDRAIRRALDGVLFID
                     EAYALAPGDERLDFGPEAIETLLKRMEDYRHRLVVIVAGYPRLMHHFLQSNPGLRSRF
                     SREIAFPDYSTEDLVAITRKLAADYEYSLDDDAVGVLRQILAGAARGEGFGNARFART
                     LFEQALNAQALRLAGVQGTELAELERSELMMLCGDDLRAAARALGEDTATDDPRSGRW
                     RRRR"
     gene            complement(3961..4302)
                     /locus_tag="VF225_00145"
     CDS             complement(3961..4302)
                     /locus_tag="VF225_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEX7143693.1"
                     /translation="MATPRAVEEAPPQETAAKPRSRATRHSGRLLLRMPAGLHEELAR
                     ASDRNGVSLNQFITSVLAGAVGWGRDDVATIGTPKVRSRRLAILLTANLLLVAAAAIS
                     AIVFLVAAWLN"
     gene            complement(4223..5020)
                     /locus_tag="VF225_00150"
     CDS             complement(4223..5020)
                     /locus_tag="VF225_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7143694.1"
                     /translation="MIASYASNHDLESRERLIEEQLPLVKALARRYAHRGEQLEDLVQ
                     VGTIGLIKAVDRYEPERGVELTAFAVPNILGEIKRHLRDRCGAIRVPRRYQETSARMR
                     SARRELAAHLQRNPTAAELATAANLSQGELAEAVRAEQARTPLSLTDAGPAVSAGEIF
                     DASEDRLLISRGLRHLRKQERLALRYRYFEDLSQEEIAARLGISQTQTSRLIASGLAK
                     LRDDFEGKSRCAVRQKALHSWHGDSEGRRGSAAAGDRGKATLARDET"
BASE COUNT          925 a         1774 c         1928 g          912 t
ORIGIN      
        1 ctgtccagcg catcagcagg ggcaggtgaa tcgacggctc gtaccagagc accttcccgt
       61 tcgccacgcg gtgctcgccg tggaagaagc cgttgtccga ggtgaagagg atgagcgtgt
      121 tgtcgagctc gccggcctgg cgcagcgtct cgacgattga cgcgaccgct tcgtccacgg
      181 acatcagcgt ctcgcggcgc tgctgccagt tctcctggat cgctgcgatc cggcgcgcat
      241 tgagcagcgg ccgctgcctg atcacccgcg gtttgtcgga cacgtccgcc tcgttgaagt
      301 tcgccggccg tggaagcggc gtgcctttca gcgcgtcgtg gtgccgcggg gccggaacag
      361 gggtcccgag ctggggcgga tcgtcgggct cacgaggcgc gccggaatgg tcgccgagga
      421 acgcgaccca caggaagaac ggcccctgcg ccgcgcggcg ccggatgatc tcgttcgcct
      481 tgtcccggta gacgtctgtc tggtagcacg acggctgcgg tgtcgcgcag aacgtgtgca
      541 gtgtcccgcc ctcgttgagg gtgtagttgt agtagcggta ggtcgtcgga tcgacggcgc
      601 cgtgccactc gctccagccc ggggggatct ccgtcgggct ctgacggccg tagccgttga
      661 ggaacttgcc gaggtgcacg gtgtagtagc cgccccgctg gagccagacg gcgagcgtgt
      721 tcgatttgtc gagcgcctga tagccgccct gcggcggcgc gttcccgcgc accccgttgt
      781 tgtgcgagta ctgcccggtg agaaaggtcg cgcgcgatgg gcagcacagc gagaacgagt
      841 cgaagttgtt gtcgaacgtg acgccctgat ccccgatcag gcgccggacg ttagggagca
      901 cttccatctc ggcgagcgtc tggtcgtcgg tctcgaggat gagcacgttc ggccgttgcg
      961 ccggcggagc cgccagcgcg gagcttccgt gttcgcgtgc tccgacggcc agtccggcgg
     1021 cgagcgccag cgtcgccgcg gttgcaagtg ccagcttgcg cttcatcgca atccccctgg
     1081 tcgcgattct gttggacttc ttattgccgc cggctgtgtg cgcccggtag agggcaggca
     1141 aaggtttcgt taagcggcga ttagctcctc ggcgggtcgc tcctcgacga gaggaacggt
     1201 tctggagcgg agccagacgt agagcgacat cgagaggccc gcaacgcctg cgaccgcgaa
     1261 cgagagctcg gtgcctccga gggccgtgag ccagccggac atgatgccgc ccagcgtccc
     1321 cgtgccggca aaggcgaaga agtagatccc gatcagccgg ccgcgcaagt gatcgggtgc
     1381 ggccaactgg atgagcgagc tcgagttcga cgaccaggtc gtgaagaaca cccctccgag
     1441 aaacaggagt agagcgatga gcgcgatcga gcgcaggggt gagatcaaca actcgcagag
     1501 cgcgaacccg gcgctcccga cggcggtcgt accgacggtt gcgcggctca cgtgggcgga
     1561 gacgagcgct ccgatcagtg cgccgacgcc gaagaccgcg gagagcagtc cgaagacctg
     1621 tggcccggag tgcagcgtgt gcttggcgag gacaggcaac aagacgttga agttgaggca
     1681 gaacgtgctc acgacgaagg tcagcatgag catgagccgg atttgctcat ggttccgggc
     1741 gaacgaaatc ccttctttga gaccgacata gagcgtgcgc ggccgcgcac ggcgctcgac
     1801 ggcgtggaac tcctgcggac gcatcatcag aaggccggca agcactgcga tatagctcgc
     1861 tgcgttgacg gcgaagcaga cgccggcgcc cgcggcggcg atgatgacgc cgccgagcgc
     1921 cgggccgaag atgcgggcgc cgttgaagac gccggagttg aggctgatcg cgttgggcaa
     1981 ctccagcgga ccgaccatgc gaaaggtcag ctgctggcgg gacggcgcgt cgaggacctg
     2041 tacgagcccc atcagggcgg cgatcacgta gacgtgccac ggccgggcga ctcccgcgag
     2101 ggcgatcacc gcgatcgtcg aggcgagcac catctgcatg agctgggtac cgatgaccgt
     2161 ccgccacgca ttgaggcggt cgacgacgac gccggcgatc aggctgaaga gcgtgaacgg
     2221 catgaactgg cagagcgcga ggatcccgac cgcgaacggc gagtgagtca gcgaaaggac
     2281 gagccaggcc tgcgcgacgc gttgcatcca cgtcccgatg ttcgagatga cctggcccgt
     2341 gaagaagagg cggtagttac ggtgtttgcg gacgctgagg aaggtacgtg caccggcgcg
     2401 aaggattgcc gccgtcaagc ctcgtcctcc gccagcaggt gcgcgagcgg ctcgatcgcg
     2461 gcgtcgatcc tctcgagctc gtcggcaccg agctcgcgta ggcgcgcggc aagccaggcc
     2521 gtccgcttcg acttgaccga gcggaggacc ttttgtccct gggcggtgag cgtcaggccg
     2581 acacgccgct ggtctccctc gacgggcgcc cgctggacga ggccggcctc ctcgaggcgc
     2641 gagatgaact tggacatgcc ggggaccgac attcgctcga gcgccgccag ctcccggatc
     2701 ccgatccctg ggtggtactt gatctggacg aggagcgaga cctggccgcc cgtcaccccc
     2761 agcgagtgaa tttcccgccg aagctgcctg ttgagctgca gtagcaccgg gcggagccgg
     2821 ttggcgagga tggtgggatc ggcggcgacg gcgatgggca ttcgttaacc aggttaacta
     2881 atcgacccac ctcgccgtcg gcttgagcgt aatccctcgt cggaggggca gtattcagcc
     2941 gtggcccgaa atccactcga agacgtggcc cgcggcttgc gggtcttggg cgagcgcctg
     3001 tcccagacaa cgggcgcgcc cgatcccagc gactggatct cggaccagct ccggcggccc
     3061 gaacctgtgc gcacgctgcc ggagcttcag gccgagctcg actccctgat cgggctcgag
     3121 acggtgaagg aacaggtccg tgcactcgtg gccttcctgc aggttcaggc cgagcggcaa
     3181 cggcacggct tgccggaggt cgctacgtcg cagcacctcg tcttcctcgg caatcccggg
     3241 accggcaaga cgaccgtcgc gcggttgctc gccgagatgt atcgcgcgat ggggctactc
     3301 aagcgcggac acctcgtcga ggtcgaccga gccggtctcg tcggtcagtt cgtcggcatg
     3361 actgcgatca agaccgaccg ggcaatccgg cgcgcgctcg acggggtgct cttcatcgac
     3421 gaggcctatg cgcttgcgcc gggcgatgag cggctggact tcgggcctga ggcgatcgag
     3481 acgctcctca agcggatgga ggactaccgc caccggctcg tggtgatcgt cgccggctat
     3541 ccgcgactga tgcaccattt cctccagtcg aatccgggac tgcgctcgcg cttctcgcgc
     3601 gagatcgcct ttccggatta ttcgacggag gatctggtgg cgatcacgcg gaagctcgcg
     3661 gccgactacg agtactccct cgacgacgac gccgtcggcg tgcttcggca gatccttgcc
     3721 ggtgctgcgc gcggcgaggg cttcggaaat gcccggttcg cacgcacgct cttcgagcag
     3781 gcgttgaacg cacaggcgct gcgccttgcg ggggtgcagg gaacggaact ggccgagctc
     3841 gagcggtcgg agctgatgat gctgtgtggg gacgaccttc gggcggcggc gcgggcgctg
     3901 ggcgaggaca cagcgaccga cgacccgcgg tcggggcgct ggcgccggcg gcgctgaagc
     3961 tcagttcagc caggcggcga ccaggaacac gatcgccgag atggcagcgg cagccaccag
     4021 caggaggttc gccgtgagca ggatggcaag ccgcctcgag cggaccttcg gcgttccgat
     4081 cgtcgcgacg tcgtcacgcc cccacccgac cgcacccgcg aggacgctcg tgatgaactg
     4141 attgagactg acgccgttgc gatcggaggc ccgcgcaagc tcttcgtgga ggcccgccgg
     4201 catccggagc agcagccggc cgctatgtct cgtcgcgcgc gagcgtggct ttgccgcggt
     4261 ctcctgcggc ggcgcttcct cgacggccct cggagtcgcc atgccacgag tgtagggcct
     4321 tctgacggac tgcacaacgc gacttccctt cgaaatcgtc gcgaagcttg gccagaccgg
     4381 aggcgatcag ccgcgatgtc tgcgtttgcg agatgccgag tcgggcggca atctcttcct
     4441 ggctgagatc ttcgaagtac cgatagcgca gtgccaatcg ctcctgcttg cgcaagtgac
     4501 gcaggccgcg ggagatgagc aggcggtcct cgctcgcgtc gaaaatctcg ccggcgctca
     4561 cagccggccc ggcgtccgtc agcgacagtg gcgtccgggc ctgctccgca cgcacggcct
     4621 cggcgagctc gccctggctg aggttcgcgg cggtggctag ctccgcagcg gtgggattgc
     4681 gttggaggtg tgccgcgagc tcccgtcgcg cgcttcgcat tcgcgcgctg gtctcctggt
     4741 agcggcgcgg tacccggatc gcgccgcaac gatcgcgcag gtgacgcttg atctcaccga
     4801 gaatgttcgg cacggcgaac gccgtgagct cgacgccgcg ctccggctcg taccggtcga
     4861 cggccttgat caggccgatc gtcccgacct gaacgagatc ctccagttgc tctcctcggt
     4921 gcgcgtaacg ccgcgcgagc gccttgacga gcggaagctg ttcttcgatc agccgctctc
     4981 gcgactcgag gtcatggtta ctcgcgtagc tcgcgatcat ccccaaattt actttagaga
     5041 ttttctttcg gatttacaaa gtcgtgggta gtcgggaccg atgtggggta gttcttctct
     5101 cggatctcca gcaacgcgaa ggtgagtgga gttcacggga atgctgctca aaggaaccaa
     5161 gctcgtgaga ctcggtgcgg ttcacgccgt cgggcttgcg gcgctgtcct tcgcaacacc
     5221 tgcacacgca acagacgatc cgcctctacc cgtaccgagc gccgcagcac ccgcacccac
     5281 ctccgcggag ccggacgaac tggagctcgg gctctcgtcg gcggtcgatg cggagattgc
     5341 tcctgtagag ccggaaagcc ctggaaatat gcttccttct gcgccggcga ctgcgcagct
     5401 gaagcgcatc cggcagggct ggagccaggt gatatcaccg acgcacgcgc agaagtcgcc
     5461 ggaacgcgcg tcgacggacg aaccacagcg gccgccggtc gccagaccgt cccacgagct
     5521 gcagtatcac cctcggcac
//
LOCUS       DASVEU010000006         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_587831,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000006 DASVEU010000000
VERSION     DASVEU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587831"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..986)
                     /locus_tag="VF225_00155"
     CDS             complement(15..986)
                     /locus_tag="VF225_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7143695.1"
                     /translation="MAKVEQKGTLSKSDLREFFREMLLIRRFEEKVEERFRAGELPGF
                     LHVAIGQEAVAVGVCRALEEGDIIASTHRAHGHTLAKGTTPNELMAELYGKLEGCSHG
                     YGGSMHLYDVERGNMGANAVVGGGLPAITGAALAFKLRGEPRVGVAFFGDGATNIGTF
                     HESLNLAQLWQVPAVFVCENNHWAESTPSWQHMPIEDISQRAVAYGMKAVKVDGQDVE
                     AVYKATRRALEHARGGKGPVFLAVNTYRLVGHYIGDPQVYRSKEELEETRKTQDPIEH
                     LRTKLELSGDEVEEIDREVQEIVEAAVEFAKNGTDPKPEDALKNVYA"
     gene            1011..1652
                     /locus_tag="VF225_00160"
     CDS             1011..1652
                     /locus_tag="VF225_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143696.1"
                     /translation="MRLVGQFNELERDLVEEWAELRLQLTVDDESHAERAAALLAPAN
                     PGRHGRVIRFVVDRRGRGIGPEALRRLLKRLDDEGIEGTLSLRGVQKAPPEELRRKET
                     LHAQWERRLSGVPADWSDIYAEVRLDSTDYLERGALLLAPVNPARFGGPAALRFRSAH
                     HFGYGVSPEMAARCFARCDEEGITGEVEILYVLSDTHPVGTQGPVWLLGGRTV"
     gene            1840..2250
                     /locus_tag="VF225_00165"
     CDS             1840..2250
                     /locus_tag="VF225_00165"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX7143697.1"
                     /translation="MSNGDPVSWFVIEPGWKVIDAEGQEIGSVDEVVGDSSDDIFNGL
                     SISTSLLGRPRYVPSEQVGTITQGHVQLKLTKDQIGRLGEFEEPPTTAEILPDGAGAV
                     RRAEAAVEAPIHSHEERLNFLTRIRHALRRALGR"
     gene            complement(2266..2727)
                     /locus_tag="VF225_00170"
     CDS             complement(2266..2727)
                     /locus_tag="VF225_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143698.1"
                     /translation="MQLGKAERQRLIESLISRKRVGTQFELLGALADAGCIVTQATIS
                     RDIRQLGLEKMHDHFGRPRYVLPHVARRTDPADVLDSILGQFGRKVTPAGNVVVLQSE
                     LGSAPAIARALDQLEHDRIVGTLAGDDTVLIISPSERDAKTLARELAGVLA"
     gene            2785..2913
                     /locus_tag="VF225_00175"
     CDS             2785..2913
                     /locus_tag="VF225_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143699.1"
                     /translation="MLRKLLWSALYGAFAALATIVSRRAASRIWRTLTGEEPPTKK"
     gene            2910..3266
                     /locus_tag="VF225_00180"
     CDS             2910..3266
                     /locus_tag="VF225_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143700.1"
                     /translation="MTADQTVERAANKLQQLANQLAGEGGLRSKLAQPLAEDADLIRK
                     MKPSLIKARAKGEAPAAKPGQATVAPSRAQLGARPKAGKKSGGPSPWIVVGAALAAGI
                     VLAKWIDWRGHAHPRD"
     gene            3247..3654
                     /locus_tag="VF225_00185"
     CDS             3247..3654
                     /locus_tag="VF225_00185"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7143701.1"
                     /translation="MPTQGTDGRPAHGLGAAVKEVAERASAIVRLELELAAMELKRKV
                     FSLGLGIALALAAAIMLLFVVGFAFATIAAALATAVSTWLALLITTGILFVFALALGV
                     VGVLKIKQGSPPVPEQAIREARLTTEALKSNGR"
     gene            3644..3931
                     /locus_tag="VF225_00190"
     CDS             3644..3931
                     /locus_tag="VF225_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143702.1"
                     /translation="MAGSNSRTLEEVRREIESERDQLAGAADTLREEIGEATNIGAKV
                     RSNLPAVAVGALGVGFLLAGGVGATARLIFRRGREGETKAKLGRFRFVDRD"
     gene            3915..>4126
                     /locus_tag="VF225_00195"
     CDS             3915..>4126
                     /locus_tag="VF225_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143703.1"
                     /translation="MSTATDRDATAGHVPSSPEKLSPAEWLAAFKSSFAAFMKDDCMG
                     LSQQIAYSSLLAFFPAVAFLVGALGL"
BASE COUNT          674 a         1389 c         1363 g          700 t
ORIGIN      
        1 ccctgtaact cagttcaggc ataaacgttc ttgagggcgt cctccggctt tggatccgtg
       61 ccgttcttcg cgaactcgac cgcggcctcg acgatctcct gcacctcacg gtcgatctct
      121 tcgacctcgt cgcccgagag ctccagcttc gtgcgtaggt gctcgatcgg gtcctgtgtc
      181 ttgcgcgtct cttcgagctc ttccttcgag cggtagacct gcggatcgcc gatgtagtgc
      241 ccgacgaggc gatacgtatt gacggcgagg aacaccggcc ccttgccacc ccgggcgtgt
      301 tcgagcgcgc ggcgcgtcgc cttgtagacg gcctcgacat cctgtccgtc gaccttgacc
      361 gccttcatgc cgtaggcgac cgcacgctgc gagatgtcct cgatcggcat gtgctgccag
      421 ctcggtgtcg actcggccca gtggttgttc tcgcagacga acacggcggg aacctgccag
      481 agctgggcca ggttgagcga ctcgtggaag gtgccgatgt tcgtcgctcc atcgccgaag
      541 aatgcgacgc cgacacgggg ctcaccccgc aacttgaagg ccagtgcggc acccgtgatc
      601 gccgggagac cgcctccgac gaccgcattc gcgcccatgt tgcctcgctc gacgtcgtag
      661 aggtgcatcg agccgccata gccgtgagaa cagccttcga gctttccgta cagctccgcc
      721 atcaactcgt tgggcgtcgt gcccttcgcg agcgtgtgtc cgtgggcgcg gtgcgtcgag
      781 gcgatgatgt cgccctcctc gagggcgcgg cagacgccga cagcgactgc ctcctggccg
      841 atcgccacgt gcaggaagcc cggaagctcc ccggccctga atcgctcctc gaccttctct
      901 tcgaaccgcc ggatcagcag catctcgcga aagaactcgc gcagatccga cttcgacagc
      961 gttcctttct gctccacctt cgccatgagc ctcagtttat gctcaggctc ttgcgactag
     1021 tcggacagtt caacgagctc gagcgcgacc tggtcgagga atgggccgag ctccgactcc
     1081 agctcacggt ggacgacgaa tcacatgccg agcgggctgc ggcgctgctt gccccggcga
     1141 atcccggtcg gcacggcagg gtcatccgct tcgtggtcga ccgccgcggc cggggaatcg
     1201 ggcccgaggc gcttcgccgg ctgctcaagc gcctcgacga cgaaggcatc gaggggacgc
     1261 tgagcctgcg gggtgtccag aaggcccctc cagaagagct gcgcaggaag gagacgctcc
     1321 acgcccagtg ggaacggcgt ctctccgggg tcccggccga ctggagcgac atctacgccg
     1381 aggtgcgact ggactccacc gactacctcg agcgtggtgc gctgctgctc gcccccgtca
     1441 atcccgcgcg cttcggcggc ccggcggcct tgcgcttccg tagcgctcat catttcggct
     1501 acggcgtgtc tcccgagatg gcggcacgat gcttcgcgcg ttgcgacgag gaggggatca
     1561 cgggcgaggt ggagatcctg tacgtgcttt ccgatacgca tcccgtcggc acacagggcc
     1621 ccgtctggct tctcgggggg cgcacggtat gacggtcgct cgccttcggc tcgctcgcgt
     1681 gagggaaacc atgtttccct cacgagctcc cttcttcagg gatcgcctca gcgtccgcgg
     1741 cgtagcgacc gccgccgcga acgctgagga taaagagagg ggaaacctcc cggtttcccc
     1801 gttcgcccct tccaccgctc atcggccaaa ggtcggccga tgagcaacgg cgacccggtt
     1861 tcctggttcg tcatcgagcc tgggtggaaa gtgatcgacg cggagggcca ggaaatcggc
     1921 tccgtcgacg aggtggtcgg ggactcgagc gacgacatct tcaacgggct ctcgatctcg
     1981 acgagcctgc tcggccggcc ccgctacgtt ccctcggagc aggtcggaac gatcacgcaa
     2041 ggacacgtgc agctgaagct caccaaggat cagatcggac gcctcggcga gttcgaggag
     2101 ccgccgacca ccgctgagat ccttccggac ggagcgggcg ccgtccggcg ggccgaagcc
     2161 gcggtcgagg cgccgattca ttcgcacgaa gaacggctca acttcctgac ccgcattcgc
     2221 cacgctttgc ggcgtgcgct cggtcgctag ccgaagacgc agaccttagg ccaggacgcc
     2281 ggcgagctca cgagcgagcg tcttcgcatc ccgctctgac ggcgagatga tgaggacggt
     2341 gtcgtctccg gcgagcgtgc cgacaatgcg gtcatgctcg agctggtcga gtgcacgcgc
     2401 gatcgccggc gcggacccga gctcggactg caggacgacg acattccctg cgggcgtcac
     2461 cttgcggccg aattgaccca ggatcgaatc gaggacatcg gcgggatcgg tccggcgtgc
     2521 gacgtgaggc agcacatacc gaggccggcc gaagtggtca tgcatcttct cgagcccgag
     2581 ctgacgaatg tcgcgcgaga tggtggcttg cgtgacgatg caaccggcat ccgcaagcgc
     2641 gccgagcagc tcgaactgcg tgccgacccg ctttcgtgag atgaggctct cgatcagccg
     2701 ctgacgctcc gccttcccca gctgcataac tccagcatac gtatgcaggt ttggcggagc
     2761 tgggccttgg tagaactcaa agtcatgctt cggaagctcc tctggtccgc gctctacggc
     2821 gccttcgccg cgctggccac gatcgtctcg cgccgcgccg cctcccggat ctggcgtacg
     2881 ctcacgggcg aggaaccgcc gaccaagaaa tgacagccga tcaaacagtc gagcgtgcgg
     2941 cgaacaagct tcagcagctc gcgaaccagc ttgcgggcga aggcggcttg aggtcgaagc
     3001 tcgcccagcc actagccgag gacgccgacc tcattcgcaa gatgaagccg agcctgatca
     3061 aggcgcgcgc caaaggcgag gcgccggcgg cgaagccggg tcaggcaacc gtggcgccgt
     3121 cgcgtgcgca gcttggtgcc cgcccgaagg cggggaagaa gagcggaggg ccgagcccct
     3181 ggatcgtcgt cggcgccgcg ctcgccgccg gcatcgtgct ggcgaagtgg atcgactgga
     3241 ggggccatgc ccacccaagg gactgacggc cgtccggctc atggtctcgg cgccgccgtc
     3301 aaggaggtcg cagagcgtgc gagcgcgatc gtgcgtctgg agctggagct cgccgccatg
     3361 gagctcaagc gcaaggtctt ctcgctcggg ctcgggatcg ccctcgccct cgcggccgca
     3421 atcatgctcc tcttcgtcgt cggcttcgca ttcgcgacga tcgcggccgc gcttgcgaca
     3481 gccgtgtcca cctggctcgc cctcctgatc acgaccggga tcctcttcgt gttcgcgctc
     3541 gcgctgggcg tagtgggagt gctgaagatc aagcaggggt cgcctcccgt tcccgagcag
     3601 gcgattcgcg aggcgaggct gacgacggaa gcgctgaaga gcaatggccg gtagcaacag
     3661 ccggacactc gaggaggttc gccgcgagat cgagtccgaa cgcgaccagc ttgcaggagc
     3721 cgccgacact cttcgcgagg agatcggcga ggcgaccaac atcggtgcca aggtgcgctc
     3781 caaccttccg gcggtagcgg tcggggcgct cggagtgggc ttcctgctcg cagggggcgt
     3841 cggggccaca gcacggctga tcttccggcg cgggcgggag ggcgagacga aagcgaagct
     3901 cggccgcttc cgctttgtcg accgcgactg accgagacgc gaccgccggg cacgtcccga
     3961 gctcgccgga gaagctgagc ccggccgagt ggctggccgc gttcaagagc agcttcgccg
     4021 ctttcatgaa ggacgactgc atgggactct cccagcagat cgcctacagc tcgctcctcg
     4081 ccttcttccc ggccgtagcc ttcctcgtcg gggcgctcgg tctctt
//
LOCUS       DASVEU010000007        18930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1366453,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000007 DASVEU010000000
VERSION     DASVEU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366453"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF225_00200"
     CDS             <1..422
                     /locus_tag="VF225_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7143704.1"
                     /translation="PMVIEAESGFHFRRRDDCLRIAMADEEPRWGFDEIVDESVFTDR
                     LERLVRRYPPAAGTNIAHAWAGLYDMTPDAHPIIGAIGDGLYAACGFSGHGFMQSPAV
                     GRAVAEELVHGESSLDLSPYRLARFSEGTTFPEHLVL"
     gene            complement(641..970)
                     /locus_tag="VF225_00205"
     CDS             complement(641..970)
                     /locus_tag="VF225_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEX7143705.1"
                     /translation="MILRFVLWNLADSKTTIQELRRYLRDESADAFADVPGLRFKAWI
                     SDEVTERWGAVYLWESAEAADQELPTGARELIGKDPDVGETFDVEATIEGRYVNEQLS
                     RLGLAFE"
     gene            complement(967..1110)
                     /locus_tag="VF225_00210"
     CDS             complement(967..1110)
                     /locus_tag="VF225_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143706.1"
                     /translation="MHTIELSEQELTLLHSALHAYLDDFGHEEADVLRELKALIAKLP
                     QPA"
     gene            complement(1119..1481)
                     /locus_tag="VF225_00215"
     CDS             complement(1119..1481)
                     /locus_tag="VF225_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7143707.1"
                     /translation="MAAYDVVDVGELEAEGPGGMVRKVRRALDAKAFGFNYFIFPPNQ
                     EGREHDHGDSNLEEVYFVVKGTGTMRVDGEEIELQPGRFVRVDPAATRLPISGADGLE
                     FVTIGAPLDRLYEPPSWG"
     gene            complement(1481..1690)
                     /locus_tag="VF225_00220"
     CDS             complement(1481..1690)
                     /locus_tag="VF225_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143708.1"
                     /translation="MRLFGRKSKRRLRELGESEAYHHSYGEPPRDVKLVKLPPRRPRY
                     RQVLADGERLRQAFAQRLDKRDKEE"
     gene            complement(1690..3252)
                     /locus_tag="VF225_00225"
     CDS             complement(1690..3252)
                     /locus_tag="VF225_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7143709.1"
                     /translation="MAVLTSQVERESEEFSRRRLRMEGLVAELRDRTAEIARGGGEAA
                     TERHRSRGKLTARERIDRLVDPGAAFLELNALAAWDLYDGEAPAAGIVTGIGIVEGRE
                     CVVVANDATVKGGSYFPLTVKKHLRAQEVAAQNNLPCLYLVDSGGAFLPLQDEVFPDR
                     DHFGRIFFNQAQMSARGIPQIAVVMGSCTAGGAYVPAMSDETVIVRGTGTIFIGGPPL
                     VKAATGQDVSAEELGGADVHTRQSGVADYHAMSDEHALALARQIVRNLQGRKPEPPWV
                     QAEPEPPAADPAELYGLIPEDFRQQTDARELIARIVDGSRFDEFKALYGETLVTGFAH
                     IEGYPVAILANNGVLFAESSQKGAHFIELATKRRIPLLFLQNITGFMVGQEYEAGGIA
                     RDGAKLVMAVANARVPKFTVIVGGSFGAGNYAMCGRAYEPRQLWMWPNARISVMGGEQ
                     AAAVLSMVGDSDPDEIRAKYEREGSPYYSTARLWDDGIIDPLDTRHVLALGLSSSLNA
                     PIAETTFGIFRM"
     gene            3483..4535
                     /gene="dinB"
                     /locus_tag="VF225_00230"
     CDS             3483..4535
                     /gene="dinB"
                     /locus_tag="VF225_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7143710.1"
                     /translation="MIVGHLDLDAFFAAVEELEDPSLKTKPLVVGGDPHGRGVVATAN
                     YVARKFGIYSAMSCAEALRRCPQTVFVRPRHSLYREYSEHVWETVRGVVPAVEKTGLD
                     EGYLDISEVAETFLEARKVAEAVQTAVRAATSLTCALGVSTSKVVAKIASDHRKPGGL
                     TVVPAGQEASFLAPFDVRRLPGVGPKAEARLVAAGVATIGSLAALGDVELRSVLPGSV
                     GRVLHDRARGIDPRGLEGQTQRISISVENTFERDLTDRTQLHAELRRMADRLSEHLRE
                     TGRVGRTVSTKLRYTDFSIRTRSTSLRVGIDEGAKIGELACALLDRALTDRPGALRLV
                     GVGVSGLTSHQQLALV"
     gene            complement(4538..5917)
                     /locus_tag="VF225_00235"
     CDS             complement(4538..5917)
                     /locus_tag="VF225_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143711.1"
                     /translation="MRMAALLGANGLMLVAGLGMLPLLGIARSWRLLLFRCGLAYLCG
                     ILLVGIVSAHLALVHVSFGWIGLGLLAALSFGFCAWRLNGTERPGWRRPDWIDAAAFA
                     ALAALTIDYGRAFRVAPLNRYDAWAIWALKGHALSAFGWADPVVLAGASYRFANLDYP
                     LLLPSLEAVDFRAMGAFDTRLLHLQFLLFLVAALLALFALLRDRVPSLVLWLSLFALA
                     LAPAVFDQLLTAYADVPLALVFGVGVGAAGRWLITNERWALTLATFCFAGALLTKNEG
                     SLFVLAVFLGLLAAAYTRWRALAVAAAIDILLLLPWRIYVHSHQLRDINYSLGDSFDY
                     EHVHGRLGVGPIAFRTLAGQMLDPQQWGLLVPIFVALFVAALLTGVRALPIFALVWTV
                     LAWLGLSWIYVISHLEYSSYLDSTKERVVASVVIGSAALVPLLAAESWARLGARGRGR
                     ARTGHGWAP"
     gene            complement(5914..6318)
                     /locus_tag="VF225_00240"
     CDS             complement(5914..6318)
                     /locus_tag="VF225_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143712.1"
                     /translation="MEGRLQGDPRPSQRPPVRLTAVAAAVLVAALAASVLVSGNEVRG
                     HVAQSVARDSKLTSAERRHAAGDRLGLDPAPFDAFRAELRPRERYAVDVPPGARGPFI
                     TRGAVVRAYAAFYFLPAIQVEQADHVFHYRFR"
     gene            complement(6261..6947)
                     /locus_tag="VF225_00245"
     CDS             complement(6261..6947)
                     /locus_tag="VF225_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7143713.1"
                     /translation="MRVSFLVPAYNEAATIVELLDRVWRLELEKQIVVVDDGSTDGTG
                     ELIEKWRSGREEVVFLRQENRGKGAAVRAAIPHADGEICVIQDADLEYDPADVPALIE
                     PIVRGSADVVFGSRLSGGKPQRVYMFWHLVGNRFLSMLTNVLYNTTLSDMETGYKAFR
                     TEILRSLDLRQNDFGIEPEITAKICKRKLRVYELPIAYYGRTYAEGKKITWRDGFKAI
                     RVLLSVRLFG"
     gene            7182..8021
                     /locus_tag="VF225_00250"
     CDS             7182..8021
                     /locus_tag="VF225_00250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX7143714.1"
                     /translation="MRLKLGLFAAVVASSFVFVGSASAITGNPVPTGDSYNYVVNLAF
                     YDSSGAYLWRCSGTLLSPTVVLTAGHCTSGAASATVWTAEGPIVDGNYPFDPVDSRPP
                     CTGYTGYPCTGYDATGAPYPYPCYNDYADFPNTCDAGIVVLDEPIYRDTYGQLPTANF
                     LDPLATQRGLKDITFTLVGYGLQSVKPVESALRERFMTTAQLVTLRSNWTNGVSVQLT
                     QDPGRGMGGTCFGDSGGPVFYGSTTTIVAVTSYGANGNCAGAGWYFRIDQPAVLEWIE
                     SFL"
     gene            complement(8108..8180)
                     /locus_tag="VF225_00255"
     tRNA            complement(8108..8180)
                     /locus_tag="VF225_00255"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8145..8147),aa:Trp,seq:cca)
     gene            8227..8403
                     /locus_tag="VF225_00260"
     CDS             8227..8403
                     /locus_tag="VF225_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143715.1"
                     /translation="MKQRPALGALFALLAIAFAGVAFAAGNGAGGQAGRWIIALAAAV
                     LAVWLASMAFRALR"
     gene            8430..9221
                     /gene="whiG"
                     /locus_tag="VF225_00265"
     CDS             8430..9221
                     /gene="whiG"
                     /locus_tag="VF225_00265"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX7143716.1"
                     /translation="MAEVEDTKSLWRLYSTNKGDKALRDRLILTYAPLVKYVAGRLGS
                     GLPAHVDEGDLVSYGLLGLIGAIERYDPDRDVKFETYAIARIKGSIIDELRAMDWVPR
                     SVRARARDIERAIAELERKLHRAPTDDEISDKLGISTDELEESLTDISRSSIGALDEL
                     WSVNTGGGDSVALIDTIEDIDAPDPQGSLSQTEMKEAIGEAIARLPEREKLVVTLYYY
                     EELTLREIGEVLGVTESRVSQLHTKAILRLKARLSGATTRASLEH"
     gene            9273..11321
                     /gene="fusA"
                     /locus_tag="VF225_00270"
     CDS             9273..11321
                     /gene="fusA"
                     /locus_tag="VF225_00270"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7143717.1"
                     /translation="MSLEPGKIRNVAVVGHRGTGKTTLVEALLFQAGATNRLGTVESG
                     STVSDWDDDEQRRQMSLSASICNVEWQGRKINLIDTPGDAGFQGDAIAALRVVEGALF
                     TVSAVMGVEVQTMRHWQRADAAGLSRVMVVNMLDRERADFYRVLEALRTQLSDKCVAV
                     HIPIGAEHELKGIVDLLHMQAYMDPEGQRESGPVDIPAEVQAEVEQYREQLLDAVVLT
                     DEGLMERYLEGQELSPEEVAKALKEAVTRGEVFPVACGVASKNLGTTAVLDLLVEGVP
                     SPAKKGAPINVDGAGTAAFVFKTIADPFAGRINVFRVLNGTLKGDSTIVNARSHSKER
                     LGQLMQLEGKEHVQVTEFGEGDIGAVAKLKETQTGDVLLDAEREIETPQIGFPEPVMS
                     FAVTPKSKGDEEKMAAGLRRLSEEDPTIQMRRDPQTGEQLLNGLSQMHVEVAVDRLKS
                     RFNVEVELHQPRVPYLETIRKESRAQGRYKKQTGGRGQFGDCVIQLEPLDGHQGYEFV
                     DKIVGGVVPQSFRPAVDKGIQEAMLHGELAGAPVQGLRVLLVDGSYHSVDSSEMAFKI
                     AGSMAFKSAYEKADPVLLEPIMELEATVPDEAVGAVNGDLNSRRGRLQGMEPQGGMTN
                     IKAEVPMAEILTYAQALTSMTGGRGDYHMHFLRYEEVPTHIAQKIIEETKKEKEEAKV
                     "
     gene            complement(11376..11567)
                     /locus_tag="VF225_00275"
     CDS             complement(11376..11567)
                     /locus_tag="VF225_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143718.1"
                     /translation="MHHARRRHHRGLGRRWYDRENVLERLENYQRDLEQELADVSDLI
                     TRLRAGEQEQPAQPQPQSV"
     gene            complement(11571..11921)
                     /locus_tag="VF225_00280"
     CDS             complement(11571..11921)
                     /locus_tag="VF225_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7143719.1"
                     /translation="MVRPGHWGVRARVERFSEPALLLLLRERPTHGYELLEALPDLTG
                     EARVDMGNLYRVLRALEEDALVTSRWESGEPGPAKRTYELTAEGRRLLDEWAAALRGS
                     RQRIDTFIDRYEGR"
     gene            12042..12656
                     /locus_tag="VF225_00285"
     CDS             12042..12656
                     /locus_tag="VF225_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143720.1"
                     /translation="MRQLARHPAKRICAVCERSLLTGERTIRFSPDGEEFVDVCALCQ
                     EAAVDYGWIKEGSPTSPTVPNQRRPRRRGIASLFDQRRQPPDDPVVAEPILRRLSVPE
                     QQMVEAADTFNSSDYRRTVGGIAKSLGEPRASIVPLSGVSGELIVTVAWDISWYQYRV
                     SPESAQPVRLAERGHEMAELDAGYQHWNARIEQDGRLVPKISPV"
     gene            12708..12938
                     /locus_tag="VF225_00290"
     CDS             12708..12938
                     /locus_tag="VF225_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143721.1"
                     /translation="MIYCVIPPELADELFDKMVEYYKDNPNVTVIIDRRKGPDRRAGK
                     KPTDGAREQREIRDRRRARIPGTFPETDAPPE"
     gene            12948..14447
                     /locus_tag="VF225_00295"
     CDS             12948..14447
                     /locus_tag="VF225_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7143722.1"
                     /translation="MALTGTKTFLNYIGGEWCDSASGETFESVNPATGDAIGTFPRSS
                     AEDVDRAVAAAKAAFDDWRLVPAPKRGEILYRFANLMMEEKDELTDLMSHEMGKVKAE
                     AGGDVQEAIDMSLYMAGEGRRLFGQTTPSELRDKFNMSVRMPIGVVGVITPWNFPIAI
                     PSWKIAPALISGNTVVFKPATDTPLLGQRFVELLVEAGLPEGVINIVHGGGGAVGDRL
                     VRHPDVPVITLTGSRETGVEVLRNAAENLKHVHLELGGKNAIIVLDDADLELAADGII
                     WSAFGTSGQRCTAASRVIVQDGVYEQLQSRLVAAAENLRLGAGWEDDTDVGPVINKKS
                     LEKIHSYTEIGKNEGANLLTGGEVATDNGFDKGNFYRPTIFGDVDPQMRIAQEEIFGP
                     TTALIRVKDFDEAIRVSNGIRYGLSSSIFTRDVNKAFRAMRDLQAGITYINAGTIGAE
                     VHLPFGGTKDTGNGHREAGQAALDVFTEWKSIYVDYSGKLQKAQIDTET"
     gene            14468..14752
                     /locus_tag="VF225_00300"
     CDS             14468..14752
                     /locus_tag="VF225_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143723.1"
                     /translation="MPREALDAVRTALFEAGAGRIGEYEHCSWYTQGTGTFFGGEGTN
                     PSLGEAGREQRVAELRLETVFPEEKQDEVIAALRRAHPYEEPAFDVYALL"
     gene            14749..15150
                     /locus_tag="VF225_00305"
     CDS             14749..15150
                     /locus_tag="VF225_00305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX7143724.1"
                     /translation="MKAKLFTDGGSRGNPGPAAYGYVIEAEDGHVLAAHGEAIGRATN
                     NVAEYSGLVAGLGKAAELGVRELEVVSDSELLVKQMQGEYRVKNEALKELWEDASDLE
                     KRFARVRYTAVRRAHNELADRLVNEALDAES"
     gene            15169..15242
                     /locus_tag="VF225_00310"
     tRNA            15169..15242
                     /locus_tag="VF225_00310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15203..15205,aa:Gly,seq:gcc)
     gene            complement(15265..15825)
                     /locus_tag="VF225_00315"
     CDS             complement(15265..15825)
                     /locus_tag="VF225_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7143725.1"
                     /translation="MVLEAQLSDAGAGLVPASTGWFVMNAHDARWFHRPGRGDSLPLT
                     GCDEFEAETYFPMLGMSIQVLPPGEPNGTYHWETEQEDFLVLAGEALLIVEGQERPLK
                     QWDFVHCPPETRHVFVGVGDAPCVILAASSRQFQKDGPWGFYCADETARSYNASSPED
                     TQDGEVAYGRFPPSRPTRYRDGLLPE"
     gene            15895..17064
                     /locus_tag="VF225_00320"
     CDS             15895..17064
                     /locus_tag="VF225_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7143726.1"
                     /translation="MRRVVSRNVGFVFVAYAFLATMIGTTLPTPLYPLFEQRYSFGEL
                     MVTVIFGVYAFGVIAGLLVFGNLSDEIGRKPVLLIGLAFSAASAFLFVFAGSLVPIFA
                     GRIASGLSAGVFTGTATAMLVDLAPGGRRRVASFVAVIVNLGGLGLGTLLAGLLADYC
                     RSPLRLPFIVDLGLLVPALVGLLMTPETVQRTAFRVRLQRLKVPTEVRGVFIRGAAAG
                     FGSFAVAGVFSSVAPVFLGQILGRTSHALAGAVVFILFTASIVGQLIVARLSDRRALV
                     SGCVLLVGGVGLLALALGLESLAALIAAASVVGLGQGLVIGAGLAAINQRAPVEHRGE
                     TASSFFVVMYVGLSLPVIGVGVAAHAFSLRTAGIGFSAAVAVLVLAVLASLTRQE"
     gene            17551..17898
                     /locus_tag="VF225_00325"
     CDS             17551..17898
                     /locus_tag="VF225_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143727.1"
                     /translation="MTNFEFDVTADGNFVTHLVTGQINEGCSPGGSLYGGDLNFGSAT
                     IPIAADGSFTIDDSYESKVDEDPATGHLTIIGHLSPGTATGTLSDTVSFTHDGTHFSC
                     GSGLQTWTAPRTG"
     gene            18126..18560
                     /locus_tag="VF225_00330"
     CDS             18126..18560
                     /locus_tag="VF225_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX7143728.1"
                     /translation="MRVYLAGPLFTPYERSFIDECARLLRADGIEVFVPHENALAAGD
                     TSAATIFAKDWQGLSDADAVVAVLDGPMVDDGTACEIGIFYALMQADSTKKGIVGLLT
                     DLRSTRGHEGHGLNLFVLGCIEAAGKVCNSMDDVAAVLAEWR"
BASE COUNT         3206 a         6210 c         6338 g         3176 t
ORIGIN      
        1 tgccgatggt gatcgaggct gagtctgggt ttcatttccg tcggcgcgac gactgcctgc
       61 gcatcgcgat ggccgacgaa gagccgcgct ggggcttcga cgagatcgtc gacgagtcgg
      121 tgttcaccga caggctcgag cggctcgttc ggcgctaccc gccggcagcg ggcacgaaca
      181 tcgcgcacgc gtgggcgggt ttgtacgaca tgacacctga tgcgcatccg atcatcggtg
      241 cgatcggcga cggcctctac gcggcgtgcg gcttctcggg gcacgggttc atgcagtcgc
      301 cggcggtcgg gcgggcggtc gcagaagagc tggttcacgg cgagtcctcg ctcgacctct
      361 cgccgtaccg tctggctcgc ttctccgagg gaacgacgtt ccccgaacat ctcgtccttt
      421 aagcaccaga cgaccgacca ggccgggtac gtcacgctga cggctgtccc gaagcagacg
      481 ttcccgctca agcgcggcta tcacgtgcag ttcttcgtgc gggtgcggaa ggacggcgag
      541 tctctgctgg ccggtgtttc cagccgtcgc ctcgcgcagg tcgcgacgac ggggtaggga
      601 tcgaatgcgg gcccgggtaa ccgggctcgc attcatcgct tcactcgaac gccagcccca
      661 gccgcgacag ctgctcgttg acgtagcggc cctcgatcgt ggcttccacg tcgaacgttt
      721 cgccgacgtc gggatctttc ccgatcagct cccgcgcgcc ggtcggcagc tcctggtcgg
      781 cggcctcggc ggactcccac agatatacgg cgccccaccg ctccgtcacc tcgtccgaga
      841 tccaggcctt gaagcgcaag cccggcacgt cggcgaaggc gtccgctgac tcgtcgcgga
      901 gataacggcg cagctcctgg atcgtcgtct tcgagtcggc gaggttccag agaacgaagc
      961 gcaggatcac gccggctgtg gcagcttcgc gatcagggcc ttgagctcgc ggaggacatc
     1021 ggcctcctcg tggccgaagt cgtcgaggta tgcgtgcagt gcggaatgca agagcgtgag
     1081 ctcttgctcg gacagttcga tcgtgtgcat tgcgcctcct agccccacga ggggggttcg
     1141 tacagacggt cgagaggagc gccgatcgtg acgaactcga gaccgtcggc gccggagatg
     1201 ggcagccgcg tcgctgccgg gtcgacgcga acgaagcggc cgggctggag ctcgatctct
     1261 tcgccgtcga cgcgcatagt gccggtgccc ttcacgacga agtacacctc ttcgaggttc
     1321 gagtcgccgt ggtcgtgctc gcgtccttcc tggttcggcg ggaatatgaa gtagttgaag
     1381 ccgaacgcct tcgcgtcgag cgcgcggcga accttcctga ccatcccgcc cgggccttcc
     1441 gcttcgagct cgccgacatc gacgacgtca tacgcggcca ctattcctcc ttgtctcgtt
     1501 tgtccagtcg ttgcgcgaat gcctgccgta ggcgctcgcc gtcggcgagc acctgtcggt
     1561 agcgcggtcg gcgcggcggc agcttgacga gcttcacgtc ccgaggcggc tcgccgtacg
     1621 agtggtggta cgcctccgac tccccgagct cgcggagacg ccgcttcgac tttctgccga
     1681 acagccgcat cacatacgga agatgccgaa cgtcgtctcg gcaataggtg cgttcaggga
     1741 cgacgagagg ccgagtgcga gaacatgccg cgtgtcgagc gggtcgatga tgccgtcgtc
     1801 ccacaacctg gctgtcgagt agtacgggct gccttcgcgc tcatacttgg cgcggatctc
     1861 gtccggatcg ctgtcgccga ccatcgagag gaccgcggcg gcctgctcgc cacccatcac
     1921 cgagatgcgc gcattcggcc acatccacag ctggcgcggc tcgtaggcgc ggccgcacat
     1981 cgcatagttg cccgctccga acgaaccgcc gacgatcacc gtgaatttcg gcacccgtgc
     2041 gttcgcgacc gccatgacga gcttcgcgcc gtcgcgcgcg atccccccgg cttcgtactc
     2101 ctgcccgacc atgaagccgg tgatgttctg caagaagagc agcgggatgc gtcgtttcgt
     2161 cgcgagctcg atgaagtgcg cgcccttctg ggacgactcg gcgaagagga cgccgttgtt
     2221 cgcgaggatc gcgaccgggt agccctcgat gtgcgcgaag cccgtcacga gcgtctcgcc
     2281 gtagagcgcc ttgaactcgt cgaagcgcga gccgtccacg atccgcgcga tcagttcgcg
     2341 cgcgtcggtt tgctggcgaa agtcctccgg gatcagtccg tagagctcgg cgggatcggc
     2401 ggccgggggc tccggctccg cctgcaccca gggcggctcg ggcttgcggc cctgcaggtt
     2461 gcggacgatc tggcgcgcca gcgcgagcgc atgttcatcg gacatcgcgt ggtagtcggc
     2521 tacaccggac tgcctcgtgt gcacgtcggc cccacccagc tcctcggcgc tcacgtcctg
     2581 ccccgttgcc gccttcacga gcggcgggcc gccgatgaaa atcgtgccgg tgccgcgcac
     2641 gatgaccgtc tcgtccgaca tcgccggcac gtaggcgccg ccggcggtgc acgatcccat
     2701 cactacggcg atctgcggga tgccccgtgc cgacatctgc gcctggttga agaagatgcg
     2761 gccgaagtgg tcgcgatcgg ggaagacctc gtcctgcaaa ggcaggaagg cgccgcccga
     2821 gtcgaccagg tagaggcacg ggaggttgtt ctgcgcggcc acctcctggg cccggaggtg
     2881 cttcttgacc gtgagcggaa agtacgagcc gcccttgaca gtggcgtcgt tggccacgac
     2941 gacgcattcc cggccctcga ctattccgat cccggtgacg atgccggccg cgggtgcctc
     3001 gccgtcgtag agatcccagg cggctagcgc attcagctcg aggaacgcgg cgccggggtc
     3061 gaccaggcgg tcgatgcgtt ctcgcgcggt gagcttcccg cgcgaccggt ggcgctcggt
     3121 cgccgcctct cctccgccgc gggcgatctc cgcggtgcgg tcgcgcaact cggcgacgag
     3181 cccctccatg cggagccgcc ggcgactgaa ctcttcggac tcgcgttcca cctgcgaggt
     3241 gaggacggcc atcgctttga gcctatacaa ggaggcctga cgcgaaattc ggccgggccg
     3301 gatgacgcac cagacgcggc gccggatcag cacgctcgcc cacggcacgt agcgccgcta
     3361 cgcaccgcga acgaccgcgc tgcccggctt ggtctggagc gcccccgacg acgaccgaat
     3421 ttcacatcag ccctcccgcg ggggcggcgg gtgccggaag gtccggggtc taggctcctg
     3481 acatgatcgt cggccacctc gaccttgatg ccttcttcgc cgcggtcgag gagctcgagg
     3541 accccagcct caagaccaag ccgctcgtgg tcggaggcga cccgcacggg cggggtgtcg
     3601 tcgcaaccgc gaactacgtt gcacggaagt tcggcattta ctcggcgatg agctgcgcgg
     3661 aagctctcag gcgctgcccg cagaccgtct tcgtgcgccc ccgccattcc ctctaccgcg
     3721 agtattcgga gcacgtgtgg gaaacggtgc gcggagtcgt tcccgccgtc gagaagactg
     3781 ggctcgacga gggttacctc gacatcagcg aggtcgcaga gacgtttctc gaggcccgca
     3841 aggtcgctga ggccgttcag accgcagtcc gggcggcgac gagcctcacg tgcgcactcg
     3901 gtgtctccac ttcgaaggtg gtggccaaga tcgccagtga ccatcgcaag cccggcggtt
     3961 tgacggtggt cccggccggt caggaggcga gcttcctggc gccgttcgac gtgcgccggc
     4021 tcccgggagt cggcccgaag gcggaggcgc gcctcgtcgc cgcgggagtc gcgaccattg
     4081 gctcgctggc cgccctcgga gacgtcgagc ttcggagcgt gctccccggc agcgtcgggc
     4141 gtgtcctgca cgaccgggcc cgcgggatcg acccacgtgg tctcgaaggc cagacgcagc
     4201 gcatctcgat cagcgtcgag aacaccttcg agcgagacct gaccgatcgc acgcagctgc
     4261 acgcggagct tcgaaggatg gccgaccgtc tctcggagca tctgcgggag accggacgtg
     4321 tggggcgcac cgtgtcgacg aagctgcgct acacggactt ctccatccgc acacggtcga
     4381 cgtctttgcg cgtcggcatc gacgaggggg caaagatcgg agagctcgcg tgcgcacttc
     4441 tggaccgcgc actcaccgac cgccccggcg cgcttcggct cgtcggagtg ggcgtttcag
     4501 ggttgacgag ccaccagcaa ctcgcgctcg tctgaggtca aggagcccat ccgtggccgg
     4561 tccgagctcg gcctcgaccg cgggcgccga ggcgcgccca ggattctgcc gcgagcaaag
     4621 gcacgagcgc cgcactgccg atcaccacgg acgcgacgac ccgctccttc gtggagtcga
     4681 ggtacgagct gtactcgagg tgagagatga catagatcca gctcaagccg agccatgcga
     4741 gcaccgtcca gacgagcgca aagatcggaa gtgcgcgcac gccggtcagc agagcggcaa
     4801 cgaacagcgc cacgaagatc ggcaccagca gcccccactg ctgtggatcg agcatctggc
     4861 cggcgagcgt cctgaacgcg atcggtccga ccccgagtct gccgtgcacg tgctcgtagt
     4921 cgaagctgtc gccgaggctg tagttgatgt cgcgcaactg gtggctgtgc acgtagatgc
     4981 gccagggaag caggagcagg atgtcgatcg cagctgcgac cgcgagcgcg cgccagcgcg
     5041 tatacgccgc ggcgaggagg ccgagaaaga cagcgaggac gaagagcgag ccctcgttct
     5101 tcgtcagtaa tgcacccgcg aaacagaacg tcgccagcgt gagggcccaa cgttcgttcg
     5161 tgatcagcca gcggccggcc gccccgacgc cgacgccgaa cacgagcgcg agcgggacat
     5221 ccgcgtaggc cgtgagcaac tggtcgaaca cggccggcgc aagcgcgaga gcgaagagcg
     5281 acagccagag gacgagcgag ggaacgcggt cgcgcaacag tgcgaagagc gccagcaacg
     5341 ccgcgacgag aaagagcagg aattgaaggt gcaggaggcg cgtgtcgaac gctcccatag
     5401 cgcgaaagtc caccgcttcg agcgaaggga gcagcaatgg gtagtccaga ttggcgaacc
     5461 ggtaggacgc gccggcgagc acgaccgggt cggcccaccc gaaggcagag agcgcatgac
     5521 ctttgagggc ccagatcgcc catgcgtcgt accgattcag cggtgcgacc cgaaaggcgc
     5581 ggccgtagtc gatcgtgagc gcggcgagag cggcgaaagc ggctgcgtcg atccaatcgg
     5641 gccgacgcca gccggggcgt tcagtgccgt tgaggcgcca cgcgcagaac ccgaacgaca
     5701 gagcggccag taacccgagt ccgatccagc caaaactcac gtgcacgagt gcaaggtgcg
     5761 cggagacgat cccgacgagc aagatgccgc agagataggc cagcccgcaa cggaagagga
     5821 gcaggcgcca cgagcgggcg atgccgagca gaggcagcat gccgaggccg gcaacgagca
     5881 tcagtccatt cgcgccgagc agcgcggcca tcctcatcgg aatcgatagt gaaagacgtg
     5941 atccgcctgc tcgacctgga tcgcaggcag aaagtagaac gccgcgtagg ccctgacgac
     6001 cgcgccccgg gtgatgaacg gtcccctcgc gccgggcggg acgtcgaccg cgtatcgctc
     6061 gcgcggccgc agttccgccc gaaacgcgtc aaagggcgcc gggtcgaggc cgaggcggtc
     6121 accggcggcg tgccgcctct cggccgacgt gagcttggag tcacgcgcaa cggactgggc
     6181 gacatggccc cgcacctcgt tcccgctcac cagcacagac gcggcgagcg ctgcgacgag
     6241 tacggcggct gcgaccgcag tcagccgaac aggcggacgc tgagaaggac gcggatcgcc
     6301 ttgaagccgt ccctccatgt gatcttcttc ccctctgcgt aggtgcggcc gtagtaggcg
     6361 atcggaagct cgtagacgcg cagcttccgc ttgcagatct tcgccgtgat ctccggttca
     6421 attccgaagt cgttctggcg gaggtcgagc gagcgcagga tctccgtgcg aaacgccttg
     6481 tacccggttt ccatgtccga cagcgtggtg ttgtacagga cgttcgtgag catggacagg
     6541 aacctgttgc ccacgagatg ccagaacatg tacacgcgct gaggcttgcc gcccgacagg
     6601 cgggagccga acaccacgtc cgcgctcccg cgcacgatcg gctcgatcag cgccggaacg
     6661 tcggccgggt cgtactcgag gtccgcgtcc tggatgacgc agatctcgcc gtcggcatgg
     6721 gggatcgcag cgcgaacggc agcacccttc ccccggttct cctgccgcag gaagacgact
     6781 tcctcgcgtc cgctccgcca cttctcgatg agctcgccgg tgccgtcagt cgagccgtcg
     6841 tccacgacga cgatctgctt ctcgagctcg agtcgccaga cgcggtcgag cagctcgacg
     6901 atggtcgccg cttcgttgta cgcggggacg agaaatgaga cgcgcacggc ggcgcagcct
     6961 accgcgcgga agaaggcgag atagagaagc ccctctgacc gacgtcccca tctcgccgcc
     7021 cggtagatgc cggttcttcg ttctttcttg cgctccgctc agccggccgg ggcgccgcga
     7081 gctgcgactc agcgagcgtc aacgttcgca caggcgagtt ggcgagccgc tactatcgcc
     7141 gcccgaagta tgttcgtggc gtcgacgagg ggggagggat tgtgcgtctg aagcttggtt
     7201 tgttcgcggc ggttgtggct tcgagcttcg tgttcgttgg atcagcctcc gctatcaccg
     7261 gcaatccagt tccgactgga gatagctaca actacgtcgt caatcttgcg ttctatgact
     7321 cgtcaggggc gtatctctgg cgttgttccg gcacgctgtt gtctccgacg gttgtcttga
     7381 ctgccggcca ctgcacgtcc ggcgcggcgt cggcgacggt ctggaccgcc gaagggccga
     7441 tcgtggacgg caactacccg ttcgatccag ttgacagtcg gccgccgtgc acgggataca
     7501 ccggctatcc gtgcaccggg tatgacgcaa cgggtgcgcc atatccgtac ccctgctaca
     7561 acgactatgc ggacttcccg aacacgtgcg acgccggcat cgtcgttctc gatgagccga
     7621 tttaccgtga cacgtacggc cagctgccaa cggcgaactt cctcgatccg ctcgcgacgc
     7681 agcgtggcct gaaggacatc acgttcacgc tcgtcggcta cggactgcag tcggtgaagc
     7741 cggtcgagtc agcgttgcgt gagcggttca tgacgacggc acagcttgtg acgttgcgga
     7801 gcaattggac gaacggtgtc agcgtccaat tgacacagga ccctggccgc ggcatgggag
     7861 gtacctgctt cggcgactcc ggaggtcctg tcttttatgg ctccacgaca acgatcgttg
     7921 cggtcacgtc gtacggggcg aatgggaact gtgcgggtgc cggatggtac ttccggatcg
     7981 accagcccgc cgtgctcgag tggatcgaga gcttccttta acgcctaggt gactagtggg
     8041 tgtaggggca gtccgcgccc ctacacccac tgaactcctg agggaggctt gagccgttcc
     8101 tcgggagcag gggcggaggg actcgaaccc ccggcctacg gttttggaga ccgccgctct
     8161 accaactgag ctacgcccct gggctgaaag gattgtagcc gtggtctttt tcgggtccct
     8221 ctggccgtga agcaacgacc agctctgggt gcgctgttcg ctctcctcgc gatcgcgttc
     8281 gcgggtgttg ccttcgcggc cgggaacggg gcagggggac aagccggacg gtggatcatc
     8341 gcgctcgcgg ctgccgtcct ggccgtctgg ctcgcgtcga tggccttccg ggcgctcagg
     8401 tagaccgaat caccgcgtat cctctgcgag tggccgaggt cgaagacacc aagtctctgt
     8461 ggcgcctgta cagcacgaac aagggcgata aggctctacg tgaccggctg atcctgacct
     8521 atgccccgct ggtcaagtac gtggccgggc ggctcggctc cggcctccct gcgcacgtgg
     8581 atgaaggcga cctcgtctcc tatgggctgt tgggtctaat cggagcgatc gagcgttacg
     8641 accccgatcg cgatgtcaag ttcgagacct acgcgatcgc gcggatcaag ggctcgatca
     8701 tcgacgagct ccgggcgatg gactgggtgc ctcgctccgt gcgtgcccgc gcgcgcgata
     8761 tcgagcgggc catcgccgag ctcgagcgca agctccatcg cgcgcccacc gacgacgaga
     8821 tctccgacaa gctcggcatc tcgaccgacg agctggagga gtcgctcacc gacatctcgc
     8881 gctcgtcgat cggcgccctg gacgagctct ggagcgtgaa caccgggggc ggcgattcgg
     8941 tcgcgctgat cgacacgatc gaggacatcg atgcgccgga cccgcaggga tcgctctcgc
     9001 agaccgagat gaaggaggcg atcggcgagg ccatcgcacg gctgcctgag cgcgagaagc
     9061 tcgtcgtgac cctctactac tacgaggagc tgaccctgcg cgagatcggc gaggtcctgg
     9121 gtgtcacgga atcccgggtc tcccagctcc acaccaaggc gatcctgcgc ctcaaggccc
     9181 gcctttcggg tgccaccacc agagcctctc tagagcacta gcaccgcgat acgatgcggc
     9241 gctcaaaccg catcgcacag aggagctggt gaatgtcact cgagcccggg aagatccgca
     9301 acgtcgccgt cgtcgggcat cgcggcacgg gtaagacgac gctcgtcgaa gccctcctgt
     9361 tccaggccgg cgccaccaac cggcttggca ccgtcgagtc aggctcgacg gtcagcgact
     9421 gggacgacga cgagcagcgg cgccagatgt ccctttcggc gtcgatctgc aacgtcgaat
     9481 ggcagggccg gaaaatcaac ctgatcgaca caccgggcga cgccggcttc cagggcgacg
     9541 cgattgccgc cctgcgcgtc gtcgagggcg ccctcttcac cgtgagcgcc gtgatgggcg
     9601 tcgaggtgca gaccatgcgg cactggcaac gcgccgacgc ggccggcctc tcgcgggtca
     9661 tggtcgtgaa catgctcgac cgcgagcgcg ccgatttcta ccgcgtgctc gaggctctcc
     9721 ggacgcagct gtccgacaag tgcgtcgcgg tacacattcc gatcggcgct gagcacgagc
     9781 tcaagggaat cgtcgatctg ctgcacatgc aggcctacat ggatcccgag ggtcagcgcg
     9841 aaagcggccc ggtcgacatc cccgccgagg tgcaggccga ggtcgagcag taccgagagc
     9901 agctgctcga cgcggtggtc ctgaccgacg aaggactgat ggagcgctac ctcgaggggc
     9961 aagagctctc cccggaagag gtcgcgaagg cgctcaagga agcggtgacg cgcggtgagg
    10021 tcttccccgt tgcctgcggt gtcgcgtcca agaatctcgg cacgaccgca gtgctcgatc
    10081 ttcttgtgga aggtgtgccc tcacccgcga agaagggcgc accgatcaac gtcgacggtg
    10141 ccggcactgc ggcattcgtc ttcaagacca tcgccgatcc gttcgcgggc cgcatcaacg
    10201 tgttccgcgt gctcaacggc acgctcaaag gcgactcgac gatcgtgaac gcgcgctccc
    10261 actcgaagga gcgcctcggc cagttgatgc agctggaagg caaggagcac gtgcaggtga
    10321 ccgaattcgg cgagggcgac attggtgcgg tcgcgaagct gaaggagacg cagaccggcg
    10381 atgtcctgct cgacgccgag cgtgagatcg agacaccgca gatcggcttc ccagagcctg
    10441 tcatgagctt cgccgtcacg ccgaagtcga aaggggacga ggagaagatg gcggcagggc
    10501 ttcggcgtct gagcgaagag gatccgacga tccagatgcg gcgcgatccg cagaccggtg
    10561 agcagctgct gaacggtctt tcgcagatgc acgtcgaggt ggcagtcgac cggctgaaga
    10621 gcaggttcaa cgtcgaggtc gagctacacc agccgcgtgt tccctatctg gagacgatca
    10681 ggaaggagtc acgcgcgcag ggccgctaca agaagcagac gggcggacgc ggccaattcg
    10741 gcgattgcgt gatccagctc gagccgctcg acggccacca gggctacgaa ttcgtggaca
    10801 agatcgtcgg cggggtcgtc ccgcagagct tccgccccgc agtcgacaag ggcattcagg
    10861 aggcgatgct gcacggcgag ctcgccggcg cgcccgttca ggggctacgc gtcctgctcg
    10921 tcgacggctc ataccacagc gtcgactcct cggagatggc attcaagatc gccggctcga
    10981 tggcgttcaa gtccgcctac gagaaggcgg accccgtttt gctcgagccg atcatggagc
    11041 tcgaggcgac cgtccccgac gaggcggtgg gcgcggtgaa cggcgatctc aactcacgac
    11101 ggggccggct gcagggaatg gagccgcagg gcggcatgac gaacatcaag gccgaggtcc
    11161 cgatggcgga gatcctcacg tacgcgcagg cgctcacttc gatgaccggc ggacgcggtg
    11221 attaccacat gcacttcctg cgctacgagg aagtcccgac gcacatcgcg cagaagatca
    11281 tcgaagagac caagaaagag aaggaagagg ccaaggtcta gtccgccgaa agtgctcagc
    11341 actttcggcg gcggcgtaga ccagcgtctt cgcgttcaga cgctctgcgg ctgtggctgg
    11401 gccggctgct cttgctcgcc ggcgcgcagt cgcgtgatca ggtcggagac gtctgcgagc
    11461 tcctgctcga ggtcgcgctg ataattctcg agccgctcga ggacgttctc ccggtcgtac
    11521 cacctgcggc cgagaccgcg gtgatgtcgt ctgcgtgcgt gatgcatggt tcacctcccc
    11581 tcatagcgat cgatgaaggt gtcgatgcgt tggcgcgacc cccggagcgc ggcagcccat
    11641 tcgtcgagca gccgcctgcc ttccgccgtt agttcgtagg tgcgcttggc cgggccaggc
    11701 tcgccggact cccagcgcga tgtgacgagg gcgtcttcct cgagcgccct caacacgcgg
    11761 tagaggttcc ccatgtcgac tcgcgcctcg cccgtcaggt cgggcagcgc ctccagcagc
    11821 tcgtacccgt gcgtcggccg ctcgcgcaag aggagcagca gggcgggctc gctgaaccgc
    11881 tccacgcggg cccggacgcc ccagtggccc ggacgaacca tgcgccggca ccgggctccg
    11941 cggccgccgc ggggcctcaa gctctcccta catgtaaaag acacatgtaa tggccgacat
    12001 tagcagttgc agcctgcgac acctgggcta gggtccacgg catgcgccag ttggcccgac
    12061 atcctgcgaa gcggatctgc gcggtctgcg agcggtcgtt actgaccggc gaacgaacca
    12121 ttcgcttctc gccggacggc gaggagttcg tcgacgtgtg cgccctctgc caggaagccg
    12181 cggtggacta cggctggatc aaggaaggga gcccgacctc tccgaccgtt ccgaatcagc
    12241 gccgcccgcg tcgccgtggg attgcgtccc tgttcgacca gcgccgccag ccgcctgacg
    12301 atcccgtcgt ggccgagccg atcctccgtc ggctgtcggt cccggagcag cagatggtcg
    12361 aggccgcgga cactttcaac tcgagcgact accgccggac ggtcggcggc atcgcgaaga
    12421 gcctcggcga gccgcgcgcg agcatcgtcc cgctctccgg ggtcagcggc gagctgatcg
    12481 tgacggtcgc ctgggacatc tcgtggtacc agtaccgcgt cagtcctgag tcggcgcagc
    12541 ccgttcgtct ggccgagcgt ggccacgaga tggccgagct cgacgccggc taccagcact
    12601 ggaacgcccg catcgagcag gacggccggc tcgttcctaa aatctctccc gtctagggcc
    12661 agcgccaccc ctctagcgcc gaccagcatc gctcttacaa tcagagcgtg atctactgcg
    12721 tgatcccgcc cgagctcgcc gatgagctgt tcgacaagat ggtcgagtac tacaaggaca
    12781 acccgaacgt caccgtgatc atcgaccggc gcaagggccc ggaccgccga gccggcaaga
    12841 agccgaccga tggggccaga gagcagcgcg agatccggga tcggcgcagg gcgcgaatcc
    12901 ccggcacctt ccccgagacg gacgctccgc ctgagtagac tcgcctcgtg gcgctgacgg
    12961 ggacgaagac cttcctgaac tacatcgggg gcgagtggtg cgactcggcc tccggcgaga
    13021 cgttcgagag cgtcaacccg gcgacgggcg acgcgatcgg cacgttcccg cgttcctccg
    13081 ccgaggacgt cgaccgggcc gtcgcggcgg ccaaggcggc gttcgacgac tggcggctcg
    13141 tgcccgcgcc gaagcgcggc gagattctct accgcttcgc gaatctcatg atggaggaga
    13201 aggacgagct cacggacctg atgtcccacg agatgggcaa ggtgaaagcg gaggctgggg
    13261 gagacgtcca ggaggcgatc gacatgagcc tctacatggc cggcgaaggc cggcgcctct
    13321 tcggccagac gacgccgtcg gagctccgcg acaagttcaa catgagtgtc cggatgccga
    13381 ttggtgtcgt cggcgtgatc acgccctgga acttcccgat cgccatcccg tcctggaaga
    13441 tcgcgcccgc gctgatctcc gggaacacgg tcgttttcaa gccggccacc gacacgccgt
    13501 tgctcggcca gcgcttcgtc gagttgctgg tcgaagccgg cttgccggag ggcgtgatca
    13561 acatcgtgca cggcgggggc ggcgccgtgg gcgatcgcct cgtccgccat ccggacgtgc
    13621 cagtgatcac gctgacgggc tcccgcgaga ccggcgtcga ggtgttgcgc aacgccgccg
    13681 agaatctgaa gcacgtccac ctcgagctcg gcggcaagaa cgcgatcatc gtcctcgacg
    13741 acgccgatct tgagctcgcg gccgacggga tcatctggtc ggcgttcgga acgtctggcc
    13801 agcgctgcac ggcggcgtcg cgcgtgatcg tgcaggacgg tgtctacgag cagctgcagt
    13861 cgcggctcgt agcggcggcg gagaacctgc ggctcggcgc cggttgggag gacgacaccg
    13921 acgtcggccc ggtgatcaac aagaaatcgc tcgagaagat ccactcgtac acggagatcg
    13981 gcaagaacga gggcgcgaac ctcctcaccg gcggcgaggt cgcgacggac aacggcttcg
    14041 acaagggcaa cttctaccgc ccgacgatct tcggcgacgt cgatccgcag atgcggattg
    14101 cgcaggagga gatcttcggg cctacgacgg cattgatccg tgtgaaggac ttcgacgagg
    14161 cgattcgcgt gtcgaacggg atcaggtatg ggctctcctc gtccatcttc acgcgtgacg
    14221 tcaacaaggc cttccgcgcg atgcgcgacc tgcaggccgg aatcacctac atcaacgcgg
    14281 gaacgatcgg ggccgaggtg catctcccgt tcggcggcac gaaggacacg ggcaacggcc
    14341 accgcgaggc gggccaagcc gccctcgacg tcttcaccga atggaagtcg atctacgtcg
    14401 actacagcgg caagctgcag aaggcgcaga tcgacaccga gacctgaacc gcaagctcgt
    14461 cgtcttcgtg ccgcgcgaag ctctcgacgc ggtacgcacg gccctcttcg aggcgggcgc
    14521 cggacgcatc ggcgagtacg agcactgctc gtggtacacg cagggaacgg ggaccttctt
    14581 cggcggcgag ggaacgaacc catccctcgg agaagccgga cgcgagcagc gcgtcgccga
    14641 gttgcgactc gagacggtct ttccggagga gaagcaggac gaggtgatcg cagcactgcg
    14701 ccgggcgcac ccgtacgagg agccggcgtt cgacgtgtac gcattgctgt gaaggcgaag
    14761 ctgttcacgg acggcggcag ccgtggaaat cccggtcccg ccgcgtacgg ctacgtcatc
    14821 gaagcggagg acggacacgt gctggccgcg cacggcgagg cgatcggacg tgcgacgaac
    14881 aacgtcgcgg aatacagcgg cctcgtcgcc gggctgggaa aagccgcgga gctaggcgtg
    14941 cgggaactgg aagtcgtgtc cgactcggag ctgctggtca agcagatgca aggcgagtat
    15001 cgagtgaaga acgaggcgct caaggagctg tgggaggatg cctcggatct ggagaagagg
    15061 ttcgcccgag tgcgctacac cgcggtgcgg cgagcccaca acgagctagc cgaccggctc
    15121 gtgaacgagg ctctggacgc cgaaagctga ggctatactg gccgcttcgc ggatgtagct
    15181 cagttggcta gagcgtctgc ttgccatgca gaaggtcgtg ggttcgagtc ccatcatccg
    15241 ctctcagaac ctgggtcgga gcacttattc cggtagcagg ccgtcgcggt agcgcgtcgg
    15301 ccgcgatggt gggaaccgcc cgtacgcgac ttcgccgtcc tgcgtgtcct cgggcgagct
    15361 cgcgttgtag ctgcgcgccg tctcgtcggc gcagtagaag ccccacggcc cgtccttctg
    15421 gaactgccgc gatgaggctg cgaggatcac gcacggcgcg tcgcccacgc cgacgaacac
    15481 gtgtcgggtc tccggcggac agtgcacgaa gtcccactgc ttgaggggcc gctcctgccc
    15541 ttcgacgatc aggagcgcct cgcccgcgag cacgaggaag tcctcttgct ccgtctccca
    15601 gtggtacgtg ccgttcggct cacccggcgg aagcacctga atcgacatgc cgagcatcgg
    15661 gaagtacgtc tcggcctcga actcatcgca tcccgtcaac ggcaagctgt cgccgcggcc
    15721 gggacggtgg aaccagcggg cgtcgtgagc gttcatgacg aaccagccgg tgctcgccgg
    15781 gacgagtccg gcgcccgcgt cacttagctg cgcctcgaga accatcgtct cgatgatagg
    15841 agccgagcct cgtcttcgcg acagcacgcc gcgccatccg ctttgctacc cgtcgtgcgc
    15901 agggtcgtca gccgcaacgt cggtttcgtg ttcgttgcct acgcgttcct cgcgacgatg
    15961 atcggaacga cgctgccgac gccgctctac ccgctcttcg aacagcgcta ctcgttcggc
    16021 gagctgatgg tgacggtgat cttcggcgtc tacgcgttcg gagtgatcgc cggcctgctc
    16081 gtcttcggga acctgtcgga cgagatcggc cggaagccgg tgctgctgat cgggctcgcg
    16141 ttctcggcgg cgagcgcgtt tctgttcgtg ttcgccgggt cgctggtacc gatcttcgcg
    16201 ggcagaatcg cctccggcct ctccgccggc gtgttcaccg ggactgccac cgcgatgctc
    16261 gtcgacctcg cgccgggagg gcgcaggcgg gtggcgagct tcgtcgcggt gatcgtgaac
    16321 ctcgggggcc tcgggctcgg cacgttactc gccgggctgc tggccgacta ctgcaggtca
    16381 ccgctccgct tgcccttcat tgtcgacctc ggattgctcg tgccggctct cgtcggtctg
    16441 ctgatgacgc cggaaaccgt gcaacgaact gcgtttcggg tgcggctgca gcgattgaag
    16501 gtgccgacgg aggtgcgagg cgttttcatc cgtggtgccg ccgccggctt cggtagcttc
    16561 gcggtcgcgg gggtgttcag ttccgtcgcg ccggtcttcc tcggccagat cctcggccgg
    16621 acgagtcacg cccttgccgg ggcggtcgtc ttcatcctct tcaccgcgtc gatcgtcggt
    16681 cagttgatcg ttgcacgcct ttcggaccgc cgagccctcg tctccggctg cgtgctccta
    16741 gtcggagggg tcgggctgct cgcgttggcg ctcggcctcg agtcgctcgc ggcgctgatc
    16801 gccgccgcgt cggtggtcgg cctcggccag ggccttgtga tcggcgccgg tctcgcggcg
    16861 atcaaccagc gcgcgccggt cgagcaccgt ggtgagaccg cctcgagctt cttcgtcgtc
    16921 atgtacgtcg gactctcgct gccggtgatc ggtgtcggcg tcgccgcgca cgcgttcagc
    16981 ctccgcacgg ccggaatcgg cttcagcgcc gcggttgcag tgctcgtgct cgcggtgctc
    17041 gcaagcctca ctcggcagga gtagcgatgc cgtcggctcg aggctgaaga ccgctggcgg
    17101 cgggcagttg agctagacac agacagcacg aagtgctttt ctctcttcgt ggggtctatg
    17161 aggggcctgt ccaaagttcg tgtcgcgctg ctgctcgtaa tcggcgcgct tgtcctagga
    17221 ggactgagca ccgccgccag tggagcgctc acgttgaccg ctccgggagt tggcggcgcg
    17281 ccgactgcgg agtcggcctc atgtaaggcg ggattcaagc cggcccgcat cggcggcaaa
    17341 ttcaagtgcc tcaagagtgg ccagccatgc gctaagcgct atcagcaggc gtaccgcaaa
    17401 tacggtttta cctgcgtcag tggtcatctc aaaaagcgtg catcgccacc cgcgtcgtct
    17461 ccgcctccaa caccaccccc cgcaccaaca cctccgccgc tcccaacgcc gcccgcccaa
    17521 cccggccatt acaagggcct cacatcgcag ttgacgaact tcgagttcga cgtgacagct
    17581 gacggaaact ttgtcacaca cctcgtcacg ggacagatca acgagggatg ctcgcccggt
    17641 ggcagcctct acggtggcga cctgaacttc gggtctgcca cgattcccat tgctgccgac
    17701 gggagcttca cgattgacga ttcgtacgag agcaaggtcg atgaagatcc cgcgaccggc
    17761 catctcacaa tcatcggcca tctatcgccg ggcacggcaa caggcacgtt gtcggacacg
    17821 gtcagcttta ctcacgatgg cactcacttt tcatgcgggt caggcctgca gacctggacg
    17881 gcgccgcgga ccggttgaag ctctttctct tcacgtagcg cctgctacgt aactgattct
    17941 gttccttctt cgttgaatca gtgcagctac cgaagaggga ggcatacgtg agcattttgt
    18001 ggatcatcct gatcgttgtg ctggtcctcg ccctgctcgg tttcgtcggg cgttaagtaa
    18061 gaccgctact gattcgcgcg gcggccttcg ggccgccgcc tttgtcttcg ggttagggtt
    18121 gcccggtgcg ggtctacctt gctggaccgc tcttcacccc gtacgagcgg tccttcatcg
    18181 acgagtgcgc gcggttgctg cgtgccgacg gcatcgaggt gttcgttccg cacgagaatg
    18241 cactcgccgc gggcgacacg tcggcagcga ccatcttcgc gaaggattgg cagggtctat
    18301 ccgatgcaga cgccgtggtc gccgtgctcg acggaccaat ggtggacgac ggcacggcct
    18361 gtgagatcgg catcttctat gcgctcatgc aggccgactc gacgaagaag gggatcgtcg
    18421 gcttgctcac cgatctccgc agcacgcgcg gccacgaggg ccacggcctg aatctgttcg
    18481 tgctcggctg catcgaggcg gcgggcaagg tgtgcaactc gatggacgat gtcgccgctg
    18541 tgctcgccga gtggcgctag ctgccgctgc gccggtcctt accggaccgc cgttcctctt
    18601 ctgaccggcg gctatcgcct tcccgcctgt cgccgtccgg cggcggtccg gcgtcgcgct
    18661 cgcgacggtc ctttccggaa cggcggtcgc ttccggatcg cctgtcgccg cctgaacgct
    18721 tttcttccgc catgtgctca tggaaggacg ctactcctac gcagtcaagc gtcctcttcg
    18781 tcttccccca gcgcatcggc gagcagagcg caggcgtagg cccgccaggc tatgccggga
    18841 tcgcgtaacc gctcgagcgc ctcggcgtcc gcatccagat gctcgactgc gcgggccagc
    18901 gcctcgcggc gttccgccga gtcgagatcg
//
LOCUS       DASVEU010000008        14350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1172414,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000008 DASVEU010000000
VERSION     DASVEU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14350
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172414"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF225_00335"
     CDS             <1..302
                     /locus_tag="VF225_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143729.1"
                     /translation="FVADGIEATIAAMERAAAGAVYNVGGGEEATMRQAIETLERVSG
                     RTLDLIERPAAAGDVRRTSADATRIKRDLGWRAMTSLADGLQAQWEWASARVAAP"
     gene            299..1123
                     /locus_tag="VF225_00340"
     CDS             299..1123
                     /locus_tag="VF225_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143730.1"
                     /translation="MSTRDVDREAEQEVDLGRYWSAIVARWWLPLAGLLVGAVIGYLI
                     SLGGSQVWKASSTIFVGAPYSVIGSVPLQGPQANPSTVGTIVRSEDAIQRAAIQAGMR
                     AGDLRGHVSTKTISGGTGTGTTRTTANPLVQITVQAPTRRKAQVAANALARLVVQRLA
                     PYTEKKIDILKQRIAADEQEVAVIERQARSSGDATAKAVFGVHLADVLDDELQAKQLL
                     VQAEEVERPKVLTRAAAVKTTARSRRNSVVVAAFLGLLIGLFAALLWEPVVRRRVR"
     gene            1090..2064
                     /locus_tag="VF225_00345"
     CDS             1090..2064
                     /locus_tag="VF225_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7143731.1"
                     /translation="MGAGRAPPRALMLEGKNVAVVVPAYDEEALVATTIQGVPDFVDR
                     IFVVDDASRDGTVAAARAAGDPRVEVVTHERNSGVGAAIVTGYQRALTEAIDVVCVMA
                     ADNQMDPSDLKKIALPVARGELDYAKANRLISGEAWRVIPRSRYLGNAVLSLLTKIAS
                     GYWHVADSQSGYTAASREILEQLDLDRIYPGYGMPNDMLVHLNVWNARVRDIPSRPIY
                     GVGERSGIKIRKVIPRISWLLVKGFFWRMREKYVIRDFHPLVFFYAFGFLMTLLGLGL
                     GIAEVVLRLMGNAVSVGTVVLVALLLIFGSQFTLFAMWFDMESNKDLR"
     gene            complement(2102..2341)
                     /locus_tag="VF225_00350"
     CDS             complement(2102..2341)
                     /locus_tag="VF225_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143732.1"
                     /translation="MARMRIRTDTAASAASLTEYLRRCECIVDVIDDRVIEAVARPQS
                     FAAPHADIELEGYLAVWQAMHPECSAERLSTLEHH"
     gene            2619..3557
                     /locus_tag="VF225_00355"
     CDS             2619..3557
                     /locus_tag="VF225_00355"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX7143733.1"
                     /translation="MPTAVVTGGAGFLGSHLCDLLVSKDYRVVCVDNLDTGSLQNVEH
                     LRGDNFLFINQDVTHHLEIEGPVDAVYHLAALASPIDYLRMPLHSLKVGSYGTHHALG
                     LAKFKRARFLLASTSEVYGDPQVHPQPETYWGNVNPIGPRGVYDEAKRYAEAMAMAYH
                     GQQGVDTAIARIFNTYGPRMRPSDGRAVPTFVRQALEGKPLTVFGDGSQTRSFCYVDD
                     LIRGLVLLAESGEHLPVNLGNPGEFTLNELAETVLRITGSESELVYEALPVDDPQVRQ
                     PDITRARQLLGWEPQIQLEDGLRRMLPTFGREPVVA"
     gene            3550..4671
                     /locus_tag="VF225_00360"
     CDS             3550..4671
                     /locus_tag="VF225_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143734.1"
                     /translation="MPRRIGVAAAIVFVAAGLCVPAASAKAHMLVGIQDDAMTLRGNP
                     TLTFQTLKQLRTQIVRINLNWNDVAKKRPAHGQDPADPAYDWDLYDRAIRYASQEGMQ
                     VLLTILFTPSWANGGRARNVPPSNYTDLQHFAYAAATRYSGHYIANTDTFDEVYLPAV
                     RYWTAWNEPANPNWLQQVSGGHFVSPRSYARICTAIWQGVHFTNFADEKVACGVTGPR
                     GNNAPRSSRPSLSPLAFMRLTRAAGLRNLDAYAHNPYYGKPSETPATKPGGSAVTLGN
                     MNTLISLVNKLWGKKRIWITEYGWQTPPDRIFGVSFARQAAYVRQSFAIARANPRIDL
                     MVWFMLRDDTNLGVGWQSGFLTAGGKKKPAFNVFARLPH"
     gene            complement(4643..5821)
                     /locus_tag="VF225_00365"
     CDS             complement(4643..5821)
                     /locus_tag="VF225_00365"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7143735.1"
                     /translation="MKILRVIARLNMGGPALHVAYLTEGLTKRGYDTTLVSGSLARGE
                     DSMAFVADARGVEVVRIDELGREISPLRDLLATIRLAKLIRRERPQILHTHTAKAGTV
                     GRVAALLAGSRKPPIIVHTFHGHVLRGYFGPLRSLFFRLLERRLAAGTTALIAVSPQV
                     RDDLVALGVAPRERFVVIRLGIELDERVATELNGRGESRRYLGVPGDRFAVGWIGRMT
                     AVKRTDDVLIAFKRLRDGGVDAVLCMVGDGPDRLQLEQRAHELGVARNTVFLGYQEDV
                     APFYAAFDALVLPSGNEGTPVSVIEALAAELPVVATRVGGVPDVVRDGEDGFLVEAGA
                     TDDLADRLAQLAGDPALRARMGKQGRERVLPRYAVERLVEDIDELYRSLLSAGGGRKR
                     "
     gene            complement(5829..6758)
                     /locus_tag="VF225_00370"
     CDS             complement(5829..6758)
                     /locus_tag="VF225_00370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7143736.1"
                     /translation="MKALVTGGAGFIGSNLVNALVERGDDVRVLDNFSTGNRANLEGL
                     DVEVVEGELRSYERVHAAVRGVEVVYHLGALGSVPRSVQDPLTSSAVNVEGTLNVLLA
                     ARDESVRRVIFSSSTSVYGSSRELPTSESTPPDPISPYGVAKLAAERYCISFGRVYDA
                     FEPVVLRYFNVFGPRQSPYSQYAAVVPLFIAAIDQAEPVTIYGDGEQSRDFTYVGNVV
                     DATIRAADAGGASGRTFNIAAGSPASVNHLAETIGVILGKPVEKRFEPPRSGDIRDSW
                     ADISAARELLGYEPRIDLAAGLELTAEAIVARV"
     gene            complement(6764..8089)
                     /locus_tag="VF225_00375"
     CDS             complement(6764..8089)
                     /locus_tag="VF225_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7143737.1"
                     /translation="MAVSETLALRARSVARSGVSPGAAALALAVPFLFLHIKYQPGVR
                     VPLGSTHLGLELSDAAVVAVAIVALREGLRAGFAPLRPALTLWIASVSLILWILVRSG
                     SLVDFVTAAKFSEYALLAFSAPLILRRRTDWELVAAATVAWSVVATGFGLLQFLGVDM
                     ADAWAAGRRQPSFLGHSDFAALSALALGIGLAAVLLAGRRIGWTAVASGGVGLILSGA
                     TAGLIGIAAGTAAFLYAVARRRRLAVRDVAVSGIVVAVVAAGVLVLRAGDFENFLRFV
                     HLKGKETQTANIQTYSHHALLAYIGYRIWLDHPIVGAGWQASTDPAVVDPELPAAHKK
                     FPDVAPLAFPTREHEWGVQNAYVQAAADLGLIGLVLWLAPFALALVLALRANAPPGAV
                     AAFTTLAAMGIWGGQGLVAGIPLDAATWLAFGLAATAVAQRSQAPPQGK"
     gene            8132..9439
                     /locus_tag="VF225_00380"
     CDS             8132..9439
                     /locus_tag="VF225_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX7143738.1"
                     /translation="MRSRLFWRRSAAAAGIYASVVLGFFGTVIVAHAFTTRMLGLYAI
                     VIAATGFFQTLLDLTIEEALVKYGFRFQEREDWGRLRRLFGRALQLKVAGGLVAGVCL
                     LALAPAAHALFGDDRLLKPMLIAAALPLVQAPENVGGVALILRGRYDIRAFFLFLSMA
                     LRLAAIAIGTRYGLTQTIAAIVVAQVLATAAVLGAGWAAFRRNPRVAAVPIGEERAEI
                     TRFVMQSSVATGVVALRSTLSPLLLGVVSSPNQVGYFRVAQTPQQGFAALSAPARLVL
                     LTEQTRAWERGTREAVFAGVRRYSLGAAAGVLVAVPLLLWLAPDLVRLFFTSKNVGAT
                     DALRLIVVAGALQFVFGWTKSFPVSIGRPKLRIWAHGIETLVLIPLVVVLGAVWGATG
                     AAGAVLASTVAFIVFWTALFLRIRREPASQGTHPSQPEAVVLP"
     gene            9436..10569
                     /locus_tag="VF225_00385"
     CDS             9436..10569
                     /locus_tag="VF225_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7143739.1"
                     /translation="MRVLIVSGIWPPDVGGPASHAPDVAEFLRGRGHEVEVVTTADAP
                     PAARPYPVRAVPRHHRAGVRHYRGAALVHHRARNADVVYTTGMFGRSAAGSALARKPY
                     VVKLTADPAFERSRRRGFVEGDVDDFQVHAGGPTVALLRFARDVELRHAAHVFTPSAY
                     LRELALGWGVAPERVSVLPNPSPVVGELGQRDELRREFGLNGATLAFAGRLTAQKSLE
                     RVLEVVAGADGVKLVIAGEGPEREPLRTRAGELGIGDRVTFLGAQPRERIVELFHAAD
                     ATILASSWENFPHTVVEALAVGTPVLAMQAGGVAEVVHDGVNGLLVAAGDTAALAEAV
                     RRYFTDDELRGRLRGAAAASVAEFAPDVVFGELEQTLRRIAGT"
     gene            10566..11669
                     /locus_tag="VF225_00390"
     CDS             10566..11669
                     /locus_tag="VF225_00390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7143740.1"
                     /translation="MKPRVLFVSRRVELPLSPSLARKWDAIGAELDYRVLAGGSGRNG
                     AFHLARELPALDGPAFFAALPLRIARELRDFKPHAVLAQGAHETAAALSARTLARSDT
                     AVIVDLHGDWRAPTRLYGSRLRGLLSPAADRVALGALRRADGIRTVTGYTTGLVRELG
                     LEPADEFPAYMDFDSFLQEAPKPLPANPQALFVGVLERYKNVDGLAEAWRLAAPRVPG
                     ARLHLVGSGTLQPLVEQLVRDLPAQTSWMERLTPGEVAAALDDSTLLVLPSRSEGMGR
                     VIVEAFCRARPVVASLVGGIPDLVQDGTNGLLVEPGDTEGLADALVRVLDVREPAERL
                     GAGAHSSADLWTVSPEDFASRLRDLVGRITGLS"
     gene            11676..12566
                     /locus_tag="VF225_00395"
     CDS             11676..12566
                     /locus_tag="VF225_00395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7143741.1"
                     /translation="MRADRFKQVLKNSVYRTIGETASAIGVNSNGERSLRVLMYHKVN
                     DLPGNPLTMPVSIFDEQMDQLRDLDYTVVDLDAVLGHYVDGNPLPERAVLITFDDGYF
                     DNLDNAAGVLRKYGYPAVLFVPIGYLDDRHPLPHEEHLAAQGILNRTIEWKELADLER
                     EGIRIESHGISHRPLADLEVDEAAREIALSKLRLEERLGRPVRAFSYVKGSEAHYKPV
                     HLSLVRQAGYDVAFTAVSGSNSQTTDPLQLRRYNIEPYSPRTFELVLAGACDLISLKD
                     TVTGTHARRIFNAALGTSTK"
     gene            12567..13454
                     /locus_tag="VF225_00400"
     CDS             12567..13454
                     /locus_tag="VF225_00400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7143742.1"
                     /translation="MSGYELEAYEPAQRDDYLRLLHDAWGDLALSGAEFDWWFKDNRT
                     GSLMSVARVDGRVAGVASHSLLRMVLGGVERLASFSVHATTEASARGLGIFPALERKH
                     EQEAEEHGVAVVLAFASKPTAPIFLGPLGWTEIGRIRVWARPFPRRPRRLEPYARVQR
                     FRHAGDAARSWPNHVVRDADYMNWRYIDSPRDYVAVAMGGSYAVLGHKEHRGTALAYV
                     ADLVGPPKPLLRACLAEVRPGSRALLAIPAPEHRAAYASLGFFPSRTTLHFMGKALAG
                     ELDTDRRAWRFTLGDTDFF"
     gene            13461..>14350
                     /locus_tag="VF225_00405"
     CDS             13461..>14350
                     /locus_tag="VF225_00405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143743.1"
                     /translation="MRRLVFLTQSVDPDHPVLAATIPKIRALAERVDEVVVLAQSGGS
                     NGFPANVEVRTFGAPSRMQRVWRFERELARALPAEAVVAHMIPAYVLLAAPLVRPRRT
                     PLLLWYTHWKASSTLSAATRLATAIVSVDRRSFPLASAKVRPIGHGIDVNEFTCTDRP
                     VRTGLRALVLGRYSPAKGIETILHAVQQVDGAEVQLHGVALNDLERRHRGDLERLGIP
                     LGDAVPRTQVPELFARSDVLVNNMEAGAPDKVVYEAAAACLPVLASNPVFDELFDGYP
                     LLFERDSPESLAERLRWFARL"
BASE COUNT         2215 a         4908 c         4881 g         2346 t
ORIGIN      
        1 cgttcgtcgc cgatgggatc gaggcgacaa ttgcggcgat ggagcgcgcc gcggccggcg
       61 ccgtctacaa cgtgggtgga ggggaggagg cgacgatgcg ccaggcgatc gagacgctgg
      121 aacgtgtgtc gggccgcacc ctcgacctga ttgagcgccc ggcggcggcc ggtgacgtga
      181 ggcgcacctc cgcggacgcg accaggatca aacgcgacct cggctggcgc gcgatgacct
      241 cgctggctga cggtttgcag gcccagtggg aatgggcctc cgctagagtc gccgcgcctt
      301 gagcacccgc gacgtcgacc gtgaagccga gcaggaggtc gatctcggcc gctactggag
      361 cgcgatcgtc gcccgttggt ggctgcccct ggcagggctg ctcgtcggcg ccgtgatcgg
      421 ctatctgatt tccctcgggg gctcacaggt ctggaaggcg agctcgacga tcttcgtcgg
      481 cgcgccgtac agcgtcatcg gcagcgtccc tctccagggc ccgcaggcga acccctcgac
      541 cgtcggcacg atcgtccgct ccgaggatgc catccagcgt gctgcaatcc aggccgggat
      601 gcgcgcgggg gacttgcgtg gccacgtttc gacgaagacg atctcgggcg gcaccggaac
      661 cgggaccacg cgcaccaccg caaatcccct cgttcagatc accgtgcagg ctccgaccag
      721 gcggaaggcg caggtcgccg cgaacgcgct tgcacggctc gtcgtacagc ggctcgcgcc
      781 gtacacggaa aagaagatcg acattctgaa gcaacgcatc gccgcggatg agcaggaagt
      841 tgccgtcatc gaacggcagg ctcggagctc cggtgacgcg accgcgaagg cggtgttcgg
      901 ggtccacctc gcagacgtcc tcgacgacga gcttcaggcg aagcaactcc tcgtccaggc
      961 cgaggaggtc gagcggccca aggtgctcac acgtgcggcc gcggtgaaga cgacggcgcg
     1021 cagccgcagg aactctgtcg tcgttgcagc gttcctcggg ttgctgatcg gactgttcgc
     1081 agcgttgctg tgggagccgg tcgtgcgccg ccgcgtgcgc tgatgctcga gggcaagaac
     1141 gtggcggtgg tcgtccccgc ctatgacgaa gaggcgctcg tagcgacgac gatccagggc
     1201 gttccggact tcgtcgaccg aatcttcgtc gtcgacgacg cgtcccgcga cggcactgtg
     1261 gcagcagccc gcgccgccgg cgacccgcgc gtcgaggtcg tcacgcatga acgtaactcg
     1321 ggggtgggcg cggcgatcgt gacgggctac cagcgcgcac tcaccgaggc gatcgacgtc
     1381 gtctgcgtga tggcagccga caaccagatg gatccgtcgg acctgaagaa gatcgccctg
     1441 ccggttgccc gcggcgagct cgactacgcc aaggccaacc gcctcatctc cggggaggcc
     1501 tggcgcgtga tccctcgctc gcgttacctc ggcaatgcgg tcctctcgtt gctgacgaag
     1561 atcgcatccg gttactggca cgttgccgac tctcagtccg gctatacggc ggccagccgg
     1621 gagatcctcg agcagctcga cctcgaccgc atctatccgg gctatggcat gccgaacgac
     1681 atgctggttc atctgaatgt ctggaacgca cgcgttcgcg acatcccgtc gcggccgatc
     1741 tacggcgtcg gcgaacggtc ggggatcaag attcgcaagg tcatcccacg gatctcctgg
     1801 ttgctcgtga aaggcttctt ctggcggatg cgcgagaagt acgtgatccg cgatttccac
     1861 ccactcgtct ttttctacgc gttcggcttc ctgatgaccc tcctcggtct cgggctcggc
     1921 atcgcggagg tcgtgctacg gctcatgggc aacgcggtgt ccgtgggcac cgtcgtcctc
     1981 gtcgcgctgc tgctgatctt cggctcccag ttcacgctct ttgcgatgtg gttcgacatg
     2041 gagtcgaaca aggatctgcg ctgaggcggc tcgacgagcg ggctctaggt gttctccgct
     2101 gttagtgatg ctcgagcgtg ctcaggcgtt cggccgaaca ctcggggtgc atcgcttgcc
     2161 agaccgcgag atagccctcg agctcgatgt ctgcgtgtgg cgccgcaaag gattgtggtc
     2221 ttgcgacggc ttcgatgacg cgatcgtcga tcacgtcgac gatgcactca cagcgccgca
     2281 gatactcggt gagggaggcg gcagaggccg ctgtgtcggt tcggattcgc attctcgcca
     2341 cacgtgattc gaccagcctg tgtctctgag cagggcccgc cgccgggccc cgctcatgag
     2401 gacacttctc tcgcgtgaaa ggctccgccg catcgagccc tcgtcacgct cgtcctgacc
     2461 atcggtgaaa tcgacaggtt gatcagtccc tcatccgcgg ttgattgggt tggttcactt
     2521 ccctggggcc agacggcgtc cctaggcccc cggccgcggc tgtaaagccc tcgtaaagtc
     2581 tgcggcctcg cgaaagacgc cccggtagca tcctgcgaat gcccaccgcc gtagtaaccg
     2641 gtggggccgg gttcctcggc tcccacctct gtgatctcct cgtctcgaag gactaccgcg
     2701 tggtctgcgt ggacaacctg gacacggggt cgctccagaa cgtcgagcac ctgcggggcg
     2761 acaatttcct cttcatcaac caggacgtga cgcatcacct ggagatcgaa gggccggtgg
     2821 acgccgtcta tcacctcgcg gctctcgcca gtcccatcga ctacctacgc atgcccttgc
     2881 actccctgaa ggtgggctcg tacgggacgc accacgcgct cgggctcgcg aagttcaagc
     2941 gtgcccggtt tttgctcgcc tcgacgagcg aggtctacgg cgacccacag gtgcaccccc
     3001 agccggagac gtactggggc aacgtcaacc cgatcgggcc ccgcggggtc tatgacgaag
     3061 cgaagcggta cgccgaggcg atggccatgg cctatcacgg ccagcagggg gtagacaccg
     3121 cgatcgcgcg gatcttcaac acctacggcc ctcggatgcg cccgagcgac ggccgcgcgg
     3181 ttccgacgtt tgtcagacag gccctggagg gcaagcccct gactgtgttc ggggacggca
     3241 gccagacgcg cagcttctgc tacgtggacg acctcattcg cgggctcgtc ctgctcgccg
     3301 agtccggcga gcacctgccc gtcaatctcg gcaatcccgg tgagttcacg ctcaacgagc
     3361 tggcggagac cgtcctgcgc atcaccggct cggagagcga gctcgtctac gaggcgctgc
     3421 ccgtcgacga tccgcaggtg cgccagcccg acatcacgcg cgcacgtcag ctgctcgggt
     3481 gggagccgca gatccagctc gaggacggcc tacgccggat gcttccgacc ttcggacggg
     3541 agccggtcgt tgcctaggcg gatcggggtc gcggcggcga tcgttttcgt cgctgccggt
     3601 ctgtgcgtgc ctgcggcctc ggccaaggcg cacatgctgg tcggcatcca ggacgacgcc
     3661 atgaccctgc gcggcaatcc cacgctcacg ttccagaccc tcaagcagtt gcgcacgcag
     3721 atcgtgcgca tcaacctgaa ttggaacgac gttgcgaaga aacgaccggc gcatgggcag
     3781 gatccggccg accctgccta cgactgggac ctctacgacc gcgcaattcg ctatgcatcg
     3841 caagagggca tgcaggttct gctcacgatc ctgttcacgc cctcctgggc gaacggtggg
     3901 cgcgcccgga acgttccacc gagcaactac acagacctgc agcacttcgc gtacgcagcc
     3961 gcgacccgct acagcgggca ctacatcgcg aacaccgaca cgttcgacga ggtctacctg
     4021 cccgcggtga ggtactggac ggcgtggaac gagccggcca acccgaactg gctgcagcag
     4081 gtgtccggcg gccactttgt cagccctcgc tcctatgcac gcatctgcac ggcgatctgg
     4141 cagggggttc acttcacgaa cttcgcggac gagaaggtcg catgcggagt gaccgggccg
     4201 cgcggcaaca atgcaccgcg tagcagccgg ccgtctctct cgccgctcgc gttcatgcgc
     4261 ctgaccaggg cggcggggtt gcggaacctc gacgcgtacg cccacaatcc gtactacgga
     4321 aagccgagcg agacgcctgc aaccaagccg ggcgggtcgg cagtgacgct cgggaacatg
     4381 aacacactga tcagcctcgt caacaagctc tggggcaaga aacggatctg gatcaccgag
     4441 tacggctggc agacgccgcc cgaccggatc ttcggcgtct cgttcgcgcg gcaggccgcc
     4501 tacgtgcgcc agtcgttcgc gatcgctcgt gcgaatccac gaatcgacct gatggtgtgg
     4561 ttcatgctcc gcgacgatac gaatctcggc gtcggctggc agtcaggctt cctcacggct
     4621 ggaggcaaga agaagccggc attcaacgtt ttcgcccgcc tcccgcactg agcagcgacc
     4681 ggtaaagctc gtcgatgtct tcgacgagcc gctcgactgc gtagcgcggc aacacgcgct
     4741 cgcggccctg cttgcccatc cgggcgcgga gggcgggatc tccggcgagc tgggcgagcc
     4801 ggtcggcgag gtcgtcggtc gctccggcct cgaccaggaa gccgtcctcg ccgtctcgga
     4861 cgacgtcggg cacgcctccg acacgcgtgg ccacgaccgg caattcggcg gccagggcct
     4921 cgatgacgct cacgggcgtg ccctcgttcc cggacgggag cacgagcgcg tcgaacgccg
     4981 cgtagaacgg cgcgacgtct tcctgatagc cgaggaagac cgtgttgcgg gcgacgccga
     5041 gctcgtgcgc gcgctgctcg agttgcaagc ggtcggggcc gtccccgacc atgcagagca
     5101 cggcgtcgac gccgccgtcg cgcaggcgtt tgaacgcgat cagtacgtcg tcggttcgct
     5161 tcaccgccgt catgcggccg atccagccga cggcgaagcg gtcgccgggg acaccgagat
     5221 agcggcggct ctcgccgcgg ccgtttagct cggtagcgac tcgctcgtcc agctcgatgc
     5281 cgagccggat gacgacgaag cgctcccgtg gagcgacacc gagtgccacg aggtcgtcgc
     5341 gcacctgcgg gctgacggcg atgagcgccg tcgtccccgc agcgagccgg cgttcgagga
     5401 gccggaagaa gagggagcgc agcggcccga aatacccgcg caggacgtgg ccgtggaacg
     5461 tgtggacgat gatcggcggc ttccgggagc cggccagcag cgcggcgaca cgaccgaccg
     5521 tgcccgcctt cgcggtgtgc gtgtgcagga tctgtgggcg ctcccgcctg atcagtttcg
     5581 cgagccggat cgtcgccagc aggtcacgga gtggagagat ctcgcgtccg agctcgtcga
     5641 tccgcacgac ctcgacgccg cgtgcgtctg caacgaaggc catcgagtct tcgccccggg
     5701 cgagcgagcc cgagacgagg gtcgtgtcgt agccacgctt cgtgaggccc tccgtcaggt
     5761 acgcgacgtg cagcgcgggg ccgcccatgt tcagccgggc gatcacccgg aggatcttca
     5821 cgcgccgttc agactctggc aacgatcgcc tcggccgtga gctcgagccc ggcggcgagg
     5881 tcgatgcgcg gctcgtagcc gagcagctcg cgcgcggcgg agatgtcggc ccaggaatcc
     5941 cggatgtctc ccgaccgcgg tggctcgaag cgcttctcga ccggcttgcc gagaatgacg
     6001 ccgatcgtct ccgcgaggtg gttgacgctg gcgggcgagc ccgccgcgat gttgaacgtc
     6061 cgtccgctcg cgccgcctgc gtccgccgca cggatcgtgg cgtcgacgac gttgccgacg
     6121 tacgtgaagt cacgcgactg ctcgccgtcg ccgtagatcg tgaccggctc ggcctggtcg
     6181 atcgccgcga tgaagagcgg tacgaccgcg gcgtactgcg agtacgggct ttgccgcggc
     6241 ccgaagacgt tgaagtacct gaggacgacg ggctcgaagg cgtcgtacac gcggccgaag
     6301 ctgatgcagt agcgctctgc ggccagcttg gcgacaccgt agggagagat cgggtcgggc
     6361 ggagtcgatt cggacgttgg cagctcccgg ctcgacccgt acacggacgt cgaagacgag
     6421 aagatcacgc gccgcacgct ttcgtcgcgg gcggccagaa gcacgttgag cgtcccttcg
     6481 acgttcaccg cactcgatgt cagcgggtcc tgcacggagc gcggcaccga gccgagcgcg
     6541 ccgaggtggt agacgacctc gactccacgc accgcagcgt ggacgcgctc gtaggagcgg
     6601 agctctccct cgacgacctc gacgtcgagg ccctcgagat tcgcgcggtt gccggtcgag
     6661 aagttgtcga gcacgcggac atcgtcgcca cgctcgacga gcgcattcac gagattcgag
     6721 ccgatgaacc ccgccccgcc tgtcaccaac gccttcatcc cggtcatttt ccctgaggag
     6781 gcgcctgact cctctgcgca accgccgtgg ccgcgaggcc gaaggcgagc cacgtcgccg
     6841 catcgagagg gattccggcc acgagaccct gcccgcccca gatgcccatc gccgcgaggg
     6901 tcgtgaaggc cgccacggct ccgggtgggg cattggcgcg cagggcgagc acgagcgcca
     6961 gcgcaaacgg tgcgagccag aggacgaggc cgatcaggcc gaggtcggct gctgcctgga
     7021 cgtaggcgtt ctgcacgccc cattcgtgct cgcgcgtcgg gaaggcaaga ggcgccacgt
     7081 cggggaactt cttgtgcgcg gccggcagct cggggtcgac gacggcgggg tcggtcgacg
     7141 cctgccagcc cgcgccgacg atcggatggt ccagccagat gcggtagccg atgtaggcga
     7201 gcagcgcgtg gtgcgagtac gtctggatgt tcgcggtttg cgtttccttg cccttgagat
     7261 gtacgaagcg caggaagttc tcgaagtcgc cggcgcgcag cacgagcact cccgcggcga
     7321 cgacagcaac cacgatcccg ctcacggcga cgtcccgcac cgcgagtcgg cgccgcctcg
     7381 cgacggcgta gaggaaagca gccgtgccgg ccgcgatccc gatcaaaccc gctgtcgcgc
     7441 cggagaggat caggcccact ccgccgcttg cgaccgccgt ccagccgatg cgcctgcctg
     7501 ccagaagcac cgctgccaga ccgatgccga gcgcgagggc cgagagcgcg gcgaagtccg
     7561 agtggccaag aaacgacggc tgccgccggc ccgccgccca cgcatccgcc atgtcgacgc
     7621 cgaggaactg cagcagtccg aagccggtcg ccacgacgct ccacgcgacg gtcgcagccg
     7681 cgacgagctc ccaatccgtc cggcgccgca ggatcagcgg ggcggaaaat gcgagcaacg
     7741 cgtattccga gaacttcgcc gccgtgacga agtccacgag cgagccagag cggacgagga
     7801 tccacaagat caacgagacc gacgcgatcc acagcgtcag cgctggacgg agaggcgcga
     7861 atccggcccg caacccctcg cggagcgcga cgatcgcgac ggccacgacc gccgcgtcgg
     7921 acagctcgag cccgaggtgc gtcgacccga gcggcaccct cactcccggc tgatacttga
     7981 tgtgcaggaa caggaagggg actgccaacg cgagggcggc ggcgccgggg ctgacgcccg
     8041 accgcgccac ggagcgcgcc ctcagcgcca gggtctccga cacggccacg ggacgagtct
     8101 aggtacagcc ctacctagaa tgatcgtcct aatgcgctcg cggctcttct ggcgccggtc
     8161 ggcggcagcc gctgggatct atgcctcggt cgtcctcggc ttcttcggga cggtgatcgt
     8221 cgcccacgcg ttcacgaccc gcatgctcgg cttgtacgcg atcgtcatcg cggcgaccgg
     8281 cttcttccag acgctgctcg acctgacgat cgaggaggcg ctggtcaagt acggatttcg
     8341 cttccaggag cgggaggact gggggcggtt gcggcgcctt ttcggccgag cactgcaatt
     8401 gaaagtcgcc gggggattgg tggccggcgt gtgtctgctc gcgctggcgc cggccgcgca
     8461 tgccctcttc ggcgacgatc gcctgctgaa gccgatgctg atcgcggccg cgttgccgct
     8521 cgtgcaggca ccggagaacg tcggcggcgt cgctctcatc ctccgcggcc gttacgacat
     8581 ccgcgcgttc ttcctcttcc tctcgatggc gctgcggctc gcggcgattg ccatcggcac
     8641 gcgctacggg ctcactcaga cgatcgctgc aattgtcgtt gcgcaggttc tggcgaccgc
     8701 ggcagtgctc ggcgccggct gggcggcgtt ccggcgcaat ccccgtgtcg ccgccgtccc
     8761 gatcggcgag gaacgcgcgg agatcacccg tttcgtcatg cagtccagcg tggcgaccgg
     8821 tgtcgtcgcg ctccgcagca cgctgagccc attgcttctg ggtgtcgtct cgtcgcccaa
     8881 ccaggtcggc tacttccgcg tcgcgcagac gccccagcaa ggattcgcag cactctcggc
     8941 gcccgcgcgg ctcgtcttgc tgaccgagca gacccgtgcg tgggagcgcg gcacgcgcga
     9001 ggcggtcttt gccggtgtcc gccgctattc gctcggcgcg gccgcgggag tcctggttgc
     9061 ggtgccgctg ctgctgtggc tggcgcccga cctggtcagg ctgttcttca cgtccaagaa
     9121 cgtcggtgcg actgatgcgc ttcgcctgat cgtcgtcgcc ggcgcgctgc agttcgtctt
     9181 cggctggacg aagagctttc ccgtctcgat cggccggccg aagctacgga tctgggccca
     9241 cggaatcgag acgctcgtcc tcatcccgct cgtggtcgtt ctcggtgcgg tctggggggc
     9301 gaccggcgcc gcaggcgcgg tgctcgcctc cactgtggcg ttcatcgttt tctggaccgc
     9361 gctcttcctg cggatccggc gcgaaccggc gagccaggga acacatccct cgcaacccga
     9421 ggcggtggtt ctgccgtgag ggtgttgatc gtctccggga tctggccccc cgacgtcggc
     9481 ggtccggcga gccatgcccc cgacgtggcg gaattcttgc gcggtcgcgg tcacgaggtg
     9541 gaggtcgtga cgacggccga cgctccgccg gcggcgcggc cgtacccggt gcgtgccgtg
     9601 ccgcggcatc atcgcgccgg cgttcggcac tatcgcggcg cggcgctcgt tcaccaccgc
     9661 gcgcggaacg cggacgtcgt ctacacgacg ggcatgttcg gccgcagcgc tgctggatcg
     9721 gccctcgcac ggaagcccta cgtcgtgaag ctcaccgccg atcctgcgtt cgagcgatcg
     9781 cgccgccgtg gcttcgtcga gggcgacgtc gacgacttcc aggttcacgc cggtggacct
     9841 acggttgcgc tgttgcggtt cgcgcgtgac gtcgagctgc ggcacgccgc gcacgtgttc
     9901 acccccagtg cgtacctgcg cgagctggca ctggggtggg gcgtggcgcc cgaacgcgtc
     9961 agcgttctgc ccaatccctc tccggtggtg ggcgagctag gtcagcggga cgagctccga
    10021 cgggaattcg gtctgaacgg tgcgacgctt gccttcgccg gccgcctgac ggcacagaag
    10081 tcgctcgagc gcgtgctcga ggtggtcgcg ggcgcagacg gcgtgaagct cgtgatcgca
    10141 ggcgagggcc cggagcgcga accgctccgg accagggccg gcgagctcgg gatcggcgac
    10201 cgagtcacgt ttctcggcgc acagccccgc gagcgcatcg tcgagctctt ccatgccgcc
    10261 gacgccacga tccttgcctc gagctgggag aacttcccgc acacagttgt cgaagcgctt
    10321 gccgtcggaa cgcccgtgct cgcgatgcaa gcgggcggcg tcgcggaagt cgtccacgac
    10381 ggcgtcaacg ggttgctcgt cgcggccgga gacacggccg cgcttgccga ggcggtccgg
    10441 cgctacttca ccgacgacga gcttcgcggg cgattgcgcg gcgctgctgc cgcctccgtg
    10501 gctgaattcg cgcccgacgt tgtcttcggc gagctggagc agaccctccg gcgaatcgcg
    10561 ggcacgtgaa gccgcgggtc ctgttcgtgt cgcggcgcgt cgagctgccc ctgtcgccga
    10621 gcctggcgcg caagtgggac gcgatcggag ccgagctcga ctaccgcgtg cttgcgggcg
    10681 gcagcggacg gaacggcgcg ttccatctgg cccgggagtt gccggcgctc gacggcccag
    10741 cgttcttcgc ggcgctgccg ctccgaatcg cccgggagct ccgcgacttc aagccgcacg
    10801 ccgtgctcgc ccagggcgcg cacgagaccg cggccgctct gagcgcgcgc acgctcgcgc
    10861 gttccgacac agccgtgatc gtggacttgc acggggactg gcgcgcaccg acccgcctgt
    10921 acgggtcgcg gctgcgcggg ctgctgagcc cggccgccga ccgcgtcgcg ctcggcgcgc
    10981 tgcgccgggc cgacggcatt cgcacggtga ccggctacac caccggcctc gtgcgcgagc
    11041 tcggcctcga gccggccgac gagtttcccg cgtacatgga cttcgactct ttcctgcagg
    11101 aggcgccgaa accgctaccg gcgaatcccc aggcgctctt cgtcggcgtg ctcgagcgct
    11161 acaagaacgt ggacgggctt gccgaggctt ggcgcctggc cgcaccgcgt gtccccggcg
    11221 cacgtctcca cctcgtcggg tccgggacgt tgcaaccgct cgtcgagcaa ctcgtccgcg
    11281 acctgcccgc gcagacgagc tggatggagc gcctgacgcc gggcgaggtc gcagcggcgc
    11341 tcgacgactc gactctcctc gtcctgccgt cgcgttccga aggtatgggc cgggtgatcg
    11401 tcgaggcctt ctgccgagcg cgaccggtcg tggcgagcct ggtgggcggc atcccggatc
    11461 tggtccagga tggaaccaac gggctcctcg tcgaaccggg cgacaccgag gggctcgccg
    11521 atgccctcgt ccgtgtcctc gacgtgcgtg agccggccga gcggctcggt gcgggagcgc
    11581 attcgagcgc tgatctctgg acggtctcgc cggaggactt cgcctcccgg ctgcgggatc
    11641 tcgtcggccg gatcaccggg ctaagctagg tcgccgtgcg cgcagaccgc ttcaagcagg
    11701 tgctgaagaa ctccgtttac cgaacgatcg gcgagaccgc gtccgcgatc ggcgtcaaca
    11761 gcaacgggga gcgcagcctg cgcgtgctca tgtaccacaa ggtgaacgac ctgcccggta
    11821 atccgttgac gatgccagtg tcgatcttcg acgagcagat ggatcagcta cgcgatctcg
    11881 actacaccgt cgtcgatctc gacgccgtgc tcggccatta cgtcgacggg aaccctttgc
    11941 cggaacgggc ggtgctgatc acgttcgacg acggctattt cgacaacctc gacaacgcgg
    12001 ccggcgtgtt gcgcaagtac ggctacccgg cggtcctgtt cgtccccatc ggctacctcg
    12061 acgaccgcca tccgctcccg cacgaggagc atctggccgc gcaaggaatc ctcaaccgca
    12121 cgatcgagtg gaaggagctt gcagacctcg agcgcgaagg gattcgcatc gagtcgcacg
    12181 ggatctctca ccgcccgctt gccgatctcg aagtcgacga agcggcgcgc gagattgcgc
    12241 tgtccaaact caggctggaa gagcggctcg gccgtccggt gcgcgcgttc tcgtacgtga
    12301 agggatcgga agcgcattac aagccggtcc atctctcgct cgtccgccag gcgggttacg
    12361 acgtggcgtt cacggcggtc tcgggctcca actcgcagac gacggacccg ctgcagctac
    12421 gccgctacaa catcgagccc tactccccgc gcacgttcga gctcgtgctg gccggggcgt
    12481 gcgacctgat ctcgctcaaa gacaccgtca cgggcaccca cgcacgtcgc atcttcaatg
    12541 cggcgctggg tacgtccacc aagtagatgt ccggctacga gctggaggcc tacgagcctg
    12601 cgcagcggga cgactacctg cgcctgctgc acgacgcgtg gggagacctc gcgctgtccg
    12661 gagctgagtt cgactggtgg ttcaaggaca accgcacggg ctcgctcatg tccgtcgccc
    12721 gcgtcgacgg gcgtgtggcc ggagtagcct cgcactcact ccttcgcatg gtgctcggcg
    12781 gcgtggagcg cctggcgagc ttctccgtcc acgcgacgac ggaagcctcg gcgcgcgggc
    12841 tcgggatttt tcctgcgttg gagagaaagc acgagcagga ggccgaggag catggcgttg
    12901 cggtcgtgct cgcctttgca tccaagccga ccgcgccgat ctttctcggc ccgctgggct
    12961 ggacggagat cgggcggatc cgcgtctggg cccggccgtt cccgcgccgg ccgcgtcgcc
    13021 tcgaaccgta cgcgcgtgtc caacggttcc gtcacgccgg cgacgcggcc aggtcatggc
    13081 cgaaccacgt ggtccgtgac gccgactaca tgaattggcg ttacatcgac tcgccgcgtg
    13141 actatgtcgc tgtcgccatg ggtggcagct atgcggtgct cggccacaag gagcaccgcg
    13201 gcacggcgct cgcctacgtc gccgacctgg tgggtccgcc gaagccgctg ctgcgcgcgt
    13261 gtctcgcaga ggtgcggccg ggctcgcgcg cactgttggc gattcccgcc cccgagcacc
    13321 gtgcggccta tgcctcgctg ggcttcttcc cgagccgcac gaccctccac ttcatgggca
    13381 aggccttggc gggcgagctg gacaccgatc gccgagcgtg gcgcttcacg cttggcgaca
    13441 cggacttctt ctaaaggccg atgcgccggc tcgtctttct cacgcagagc gtcgatccgg
    13501 accatccggt tctggccgcc accatcccga agatccgtgc gctcgcggag cgtgtggacg
    13561 aggtggtcgt cctcgcacag tcgggcggca gcaatgggtt tcctgcgaat gtggaggtgc
    13621 ggacgttcgg agcgccctca cgcatgcagc gcgtctggcg gttcgaacgc gagctcgcgc
    13681 gtgcgttgcc ggcggaggcg gtcgtcgcgc acatgattcc ggcctacgtc ctcctcgcgg
    13741 cgccgctcgt gcgtccgcgg cggacgccgc tgctgctctg gtacacccac tggaaggcat
    13801 cctccacgct cagtgccgcc acgagactcg cgacggcgat cgtgagcgtc gaccggcgct
    13861 cgtttccact ggcatcggcc aaagtccggc cgatcgggca cggtatcgac gtgaacgaat
    13921 tcacgtgcac ggatcgaccc gtgcgtacgg gcctgcgggc tctcgtgctc ggacgctact
    13981 cgccggccaa ggggatcgag acgatccttc acgcagtgca gcaggtcgac ggcgcggaag
    14041 tccagctgca cggcgtcgcg ctgaacgacc tcgagcgacg gcatcgcggt gacctcgagc
    14101 gcctcggcat tccgctggga gacgccgtgc cgcggacgca ggttccggag ctgttcgcgc
    14161 gctctgacgt tctcgtcaac aacatggagg cgggagcgcc ggacaaggtc gtgtacgaag
    14221 ccgcagcggc ctgtctgccg gtgcttgcgt ccaatcccgt cttcgacgag ctcttcgacg
    14281 gctatccgct gctcttcgag cgcgactcgc cggaaagcct ggccgagcgc ctgcgctggt
    14341 ttgcccgcct
//
LOCUS       DASVEU010000009         9127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_397201,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000009 DASVEU010000000
VERSION     DASVEU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397201"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..931
                     /locus_tag="VF225_00410"
     CDS             74..931
                     /locus_tag="VF225_00410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143744.1"
                     /translation="MGFLHRHRNLTPYLLLAPGLLYLGFFFVVPLGFLAYQSLESGNF
                     DVGYAFTWAWGNYWDAIHNYSGHFVRSLEYAGIATILALVFGYPLAYWIAFRGGRWKN
                     LLLLFIVAPFFVTYLIRTLAWETILADQGPVVTVLRHIGILGHDGRLLATSTAVVAGL
                     TYNFFPFMALPLYVSLEQIDGRLIEAAQDLYASKWKAFLRITLPLSLPGVVAGTLLTF
                     IPAAGDFINAQLLGSPQNHMIGNVIQSKFLELTDYPAAASMSFVLMAAILLMVALYAR
                     AVGTEKLTG"
     gene            928..1782
                     /locus_tag="VF225_00415"
     CDS             928..1782
                     /locus_tag="VF225_00415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143745.1"
                     /translation="MTTATLEQGQSGENALARVWALVKHHVLTVYSLLAFVYLMLPIG
                     VVIAFSFNHPQGRFNYVWSGFTLDNWKNWDGVPGLRSAMVLSLEIAVLASLVATALGM
                     LIALALVRYGFRGRGLTNLLIFLPLSSPEIVLGASLLTLFLNLKVVFGFWTILIAHVM
                     FCISFAVVTIKARLIGFDRHLEEAAMDLGANEWATFRKVTLPLIAPAILAALLLCFAI
                     SIDDFVVTYFNSGPRTTFPLFVWGAARVGAPPQVNVIGTVIFGVAVIGMVANVLVQMR
                     RERAAATA"
     gene            1779..3179
                     /locus_tag="VF225_00420"
     CDS             1779..3179
                     /locus_tag="VF225_00420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7143746.1"
                     /translation="MSAPSVAPRLPPWWLDEAGPQTAAPRLDGDTAADVCIVGGGYTG
                     LWTALALKERDPSLEVVLVESEICGAGPSGRNGGFLHGYWAGLADLLPLLGRERALQL
                     AHAGERIVPAVRAFCESRGEDVWLRESGILMVSASPSQDDAIDNAVAAAAQVDAGEQA
                     LPLDVNGVAERIRSPVFRRGVYFPDGATVQPARLVHALRRAALEAGVVLYEQTPALRI
                     RAGQVATPRGSVGAPEIVVAINAAATGWRPVARHVTNFGSYVVLTEPVPELLEEIGWT
                     GGEAVLDARMFLHYFRTTPDGRVLMGSGSGPIGFRGRVDGRFTADDATAARAEHGLRR
                     LLPGLAEARIERAWGGPIDVSADHLPFFGTVKGKRIHYGLGYSGHGVGPTWLGGQILA
                     SLVQERDDEWTALPLASRRVASLPPEPLKHLGGALVRSSILACERADEESRRPPLPAR
                     LGAALPRVFGMEIGTR"
     gene            complement(3176..3445)
                     /locus_tag="VF225_00425"
     CDS             complement(3176..3445)
                     /locus_tag="VF225_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143747.1"
                     /translation="MRGVLVGAVLIFALMLVVKDGRASRHLGLTGSCQTVAAPAGDDG
                     AWAACKPGKLEGAPDLTRHGCKSVRFVDQRELWRCPADIKSSNDT"
     gene            complement(3484..4701)
                     /locus_tag="VF225_00430"
     CDS             complement(3484..4701)
                     /locus_tag="VF225_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143748.1"
                     /translation="MELRRWPRKNDPESIEARQKALGLSDGVTRIDLAPFVNAQEMLT
                     GAAVIPVSVVGPLEIELGDYELEEPFGRVIETSRNQDQVFVPLAHTEGGLSASLYRGA
                     RAAAESGGFRTYVLGDRMTRASCFVCKSTEEAVQLARFLDAHVAEMRRWLAERNEDWI
                     SKFARLREVETHVIGPMCHVLWAFTTGDACGPNMMTRNAYALNMGYVMEHAPVRPQRA
                     ILEANMGGDKKPSHRYFERGGHGKTVLVEATLTEEAVRRVLRTTVDDLLELSYVGTHG
                     AVASGMQSVAFTPASAIAAIFAATGQDLGMVGTSSMAHGTARRVDGGIQCGLRLPGLE
                     VGTVGGGTIFPYAHTWLELMDCAGPGKVYRFTQIVAAATLALEISASAAMATAGSENF
                     FKAHHERGGLRAS"
     gene            4844..5659
                     /locus_tag="VF225_00435"
     CDS             4844..5659
                     /locus_tag="VF225_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7143749.1"
                     /translation="MATGQLIEMKDEGREAFADVLRFYELAKRREWQVRDLPWGELPP
                     IPEAKGSPQSQARRRDMWRSVLTQQLQADMLACDMASQLLNMAPDVEAKLYYSTMVQD
                     ESRHTEAWLKLVIEAGGTAERDPHLDKLAKTTLEADTLEEKVFLMQVFYERLIISRFR
                     LIARSSRGTVLEDLCGRLATDDGIHHGAGMAYERVLLQNLSKKMNQKLVDAANRLLPV
                     FAAHALWRPRERAWIGRAMEARDVERLREDLELGVRLAESLGLDVSGVELTIP"
     gene            5668..6522
                     /locus_tag="VF225_00440"
     CDS             5668..6522
                     /locus_tag="VF225_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX7143750.1"
                     /translation="MNATLMDGAALAVRLREEIKHEVAELGHVGLATVLVGDDPASEV
                     YIRLKHKAADEAGIEAIDHRLPATTTEDELVELVQHLHKDDFVDGILVQTPLPDQIDE
                     ARVMLAIDPMKDVDGLHPFSAGQLYLGRPTLVPATPLGVMHLLDEYKIPIAGSRAVVV
                     GRSSLVGKPMALLLLHANATVTVCHSRTQDLARHTLDADLLVAAVGLPDVITADMVKQ
                     GATVIDVGITRTEAGLVGDVEEDVAEVAAFLTPVPGGVGPMTIAALLGNAVRAARYRR
                     GKLAFPRI"
     gene            6598..6798
                     /locus_tag="VF225_00445"
     CDS             6598..6798
                     /locus_tag="VF225_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7143751.1"
                     /translation="MAQGTVKWFSNEKGYGFIEREGGEDVFVHFSAITQEGYKSLTEG
                     QRVEFEVVQGPKGAQAANVQPV"
     gene            6979..7284
                     /gene="gatC"
                     /locus_tag="VF225_00450"
     CDS             6979..7284
                     /gene="gatC"
                     /locus_tag="VF225_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7143752.1"
                     /translation="MAISREDVLHVAALAQLELSEDEIERLIEQLNDILAAVGKVSEL
                     DLSDVPPTSHPLSVVNVFRPDVPRPSLLLDDAFANAPERDRDLFRVPPTGATEEPSS"
     gene            7281..8684
                     /gene="gatA"
                     /locus_tag="VF225_00455"
     CDS             7281..8684
                     /gene="gatA"
                     /locus_tag="VF225_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7143753.1"
                     /translation="MIDTLRLTAEDAKSLLERGEVSGAELRAAYREAIDGRNEELNSF
                     LTLVDGDDNDGVPIALKDVISTKGVRTTAGSKILEDYVPVFDSTVADRCKAAGLQLLG
                     KTNTDEFAMGSSTENSAYGPSRNPWDPTRVPGGSGGGSAAAVSAGLAPWALGSDTGGS
                     IKQPSALCGNVGLRPTYGTVSRYGIVAFASSLDQVGPVTRTVRDNALLYGIISGRDEC
                     DSTTVDVPPVELPEAEDLKGLRVGVPKELNEADGISPGVAEAVRRAIDLCSELGADVG
                     ECELPRSVEYGLACYYLIAPAEASSNLARYDGVRFGLRVPAENYYEMVARTRDAGFGD
                     EPKRRIMLGTYALSAGYYEAYYGQAQKVRTVIAREFAEAFERFDVLVSPTSPTVAFKI
                     GEKADSPLAMYLTDVLTIPPNMAGLPGLSIPCGLSEGLPVGLQLIGAQFSENTLFRTG
                     HALEHAIGFDTVPERLR"
     gene            8782..9030
                     /locus_tag="VF225_00460"
     CDS             8782..9030
                     /locus_tag="VF225_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143754.1"
                     /translation="MRTIVDRAADFVLAVERVFGTPPRVLDGSRAVQIDDVRLSLEAG
                     ERELCLIRMHGLLEEYLAIFEVRGDIEVPLLRAKEFLA"
BASE COUNT         1456 a         3042 c         3004 g         1625 t
ORIGIN      
        1 gcgaatgcgc tgaacaacca gaaatacatc accgcctggc aaaacctcat ctcaggctga
       61 tctagggact ggcgtgggat tcctgcaccg tcatcggaat ctcacgccgt acctcctgct
      121 tgcgcccggg ttgctgtatc tcgggttctt cttcgtcgtc ccgctcggtt ttctcgccta
      181 tcagtcgctc gaatcaggca acttcgacgt cggctatgcg ttcacgtggg cctgggggaa
      241 ctactgggac gcgatccata actacagcgg tcatttcgtc cgctcactcg aatacgccgg
      301 aatcgcgaca atcctggcgc tggtcttcgg ctacccgttg gcgtactgga tcgcgtttcg
      361 cggtggccgc tggaagaacc tgctcctcct gttcatcgtc gcgcccttct tcgtcacgta
      421 tctgatccgg acgctcgcct gggagacgat cctcgccgac cagggcccgg tcgtcaccgt
      481 cctgcggcac atcgggattc tcggccacga cggccggttg ctcgccacgt cgaccgcggt
      541 ggtggcgggc ctgacttaca acttcttccc gttcatggcc ttaccgctct acgtgtcgct
      601 cgagcagatc gacgggaggc tgatcgaggc cgcgcaggat ctttatgcga gcaagtggaa
      661 ggcgttcttg cggatcaccc tgccgctctc tttgccgggt gtcgtcgcgg ggacgctgct
      721 gacgttcatc cccgctgcgg gcgacttcat caacgcccag ctcctcgggt cgccacagaa
      781 tcacatgatc ggaaacgtca tccaatcgaa gttcctcgag ctcaccgact acccggccgc
      841 cgcctcgatg tcattcgtcc tgatggccgc gattctcctg atggtggcgc tctacgcccg
      901 cgctgtcgga accgagaagc tcacaggatg acgaccgcga ctctggagca aggacagagc
      961 ggggagaacg cgctcgcacg cgtgtgggcg ttggtcaagc accacgtcct gacggtgtac
     1021 tcactgctcg ccttcgtgta cctgatgctg cccatcgggg tcgtgatcgc attttcgttc
     1081 aaccatccgc aaggccgctt caactacgtc tggtcgggct tcactctgga caactggaag
     1141 aactgggacg gcgtgcccgg cctgcgcagt gcaatggtcc tgtcgctcga gatcgcggtg
     1201 ctggcaagcc tggtcgcgac cgcgctcgga atgctgatcg cgcttgccct cgtgcgctac
     1261 ggcttccgcg gccgcggcct gaccaacctc ctgatcttcc tgccactgtc gtcaccggag
     1321 atcgtcctcg gagcttcgct gctgacgctc ttcctcaacc tcaaggtcgt cttcggcttc
     1381 tggacgattc tgatcgcgca cgtgatgttc tgcatcagct tcgccgtcgt cacgatcaag
     1441 gcgcgtctga tcggcttcga ccgccacctc gaggaggcgg caatggacct cggcgcgaac
     1501 gagtgggcga cgtttcgtaa ggtgacgctg cctctgatcg ccccggcgat ccttgctgcg
     1561 ctcctgctct gcttcgcaat ctcgatcgac gacttcgtcg tgacctactt caactccggc
     1621 ccgcgtacga cgttcccgct cttcgtctgg ggtgcggcac gcgtcggcgc gccgccgcag
     1681 gtgaacgtga tcggcaccgt gatcttcgga gtggccgtga tcgggatggt cgcgaacgtg
     1741 ctcgttcaga tgcgccgcga acgagcagcc gcgacagcgt gagcgcccca tccgtcgctc
     1801 cacgcctccc accgtggtgg ctcgacgaag ctgggcccca aacggcggcg cctcgactgg
     1861 acggcgacac cgctgccgat gtctgcatcg tcggcggcgg ctatacgggc ctctggacgg
     1921 ccctggcttt gaaagagcgc gatccatcgc tagaggtcgt gctcgtggag tcggagattt
     1981 gcggcgccgg gccgagcggg cgcaacggcg gattcctgca cggctactgg gccgggctcg
     2041 ccgatctatt gcccttgctc ggacgcgagc gcgctttgca gctcgcccat gccggggagc
     2101 ggatcgtgcc cgccgtgcgc gcgttctgcg agtcgcgcgg ggaggacgtc tggttgcgtg
     2161 agtcggggat cctgatggtc tccgcatcgc cgtcccagga cgatgcgatc gacaacgcgg
     2221 ttgccgctgc cgcccaagtg gatgcaggcg agcaagcctt gccgctcgac gtgaacggtg
     2281 tcgccgagcg aatccgctct ccggtcttcc gtcggggcgt ttacttcccg gacggtgcga
     2341 ccgtgcagcc ggcacggctc gtgcacgcgc tgcgccgcgc tgctctggag gcgggagtcg
     2401 tgctctacga gcagacacct gccctacgca tacgtgcagg gcaggtggcc acgccccgcg
     2461 gcagcgtcgg cgcgccggag atcgtcgttg caatcaacgc tgctgccacc ggctggcggc
     2521 cggtcgctcg ccacgtgacg aacttcggct cgtacgtggt cctgacagag cccgttcccg
     2581 aattgctgga ggagatcggc tggaccggcg gcgaagccgt cctcgacgcg cgcatgttcc
     2641 tgcactactt ccgcacgact ccggatgggc gtgtgctgat gggcagcggt tccggcccga
     2701 tcggatttcg cggacgcgtg gacgggcgct tcaccgcgga cgatgcgacg gccgctcgag
     2761 cggagcacgg gctgcggcgt ctcctgcccg gcctggccga ggctcgaatc gaacgcgcgt
     2821 ggggaggacc gatcgacgtg tcggccgacc atctcccgtt cttcgggacg gtgaagggca
     2881 agcggatcca ctacggcctc ggctattcag ggcacggtgt cggtccgacg tggctcggcg
     2941 gtcagattct cgcgtcgctc gtccaggagc gcgacgacga atggacggca ttgcccctcg
     3001 cctcgcggcg cgtggcgtcc ctaccgccgg aaccgctcaa gcatctcggc ggcgcgctcg
     3061 tgcggtcgtc gatcctcgcc tgcgagcggg cggacgagga gagccgccgg ccgccgttgc
     3121 cggcgagact cggtgccgca ctgccgcgcg tattcggaat ggagatcggg acgcgttaag
     3181 tgtcgttgct gcttttgatg tcggcggggc agcgccacag ctcgcgctgg tcgacgaagc
     3241 gcacggactt gcagccgtgc ctagtgaggt cgggcgcgcc ctcgagcttg ccgggcttgc
     3301 acgctgccca ggcgccgtcg tctcctgcgg gcgcggcgac ggtctgacag ctgccggtca
     3361 gaccgaggtg acgcgatgcg cggccgtcct tgacgactag catcaaggcg aagatcagaa
     3421 ccgcccctac cagcacgcct cgcatgcagt gaatatcggc attcgaggca tctgcctcaa
     3481 gccctaagaa gcgcgcaggc cgcctcgctc atgatgcgcc ttgaagaagt tctcggaccc
     3541 ggcggtcgcc attgccgcac tcgctgagat ctcaagggcc agcgtcgcgg ccgccacgat
     3601 ctgcgtgaag cggtacacct tgccggggcc tgcgcagtcc atcagttcga gccacgtgtg
     3661 cgcgtagggg aaaatcgtcc cgcccccgac ggtgccgacc tcgaggccag gcaggcgtag
     3721 cccgcactgg attcccccat cgacgcggcg ggccgtgccg tgggccatcg agctcgttcc
     3781 gaccatgccg aggtcctggc cggtcgctgc gaagatcgca gcgattgccg atgccggtgt
     3841 gaacgcgacc gattgcatgc cggatgcgac cgctccgtgt gttccgacgt acgagagctc
     3901 gagcagatcg tcgaccgttg tgcgaaggac gcggcgcacc gcctcctccg tgagcgtcgc
     3961 ttccacgagg acggtcttcc cgtgcccgcc tcgctcgaag taacggtgcg acggcttctt
     4021 gtccccgccc atgttcgctt cgaggattgc gcgctggggt ctcaccggcg cgtgctccat
     4081 cacgtagccc atgttcagcg cgtacgcgtt gcgcgtcatc atgttcggcc cgcacgcatc
     4141 gccggtcgtg aaggcccaga gaacgtgaca catgggaccg atcacatgcg tctcgacttc
     4201 gcgcaggcgc gcgaacttcg agatccagtc ttcgttccgc tccgccagcc agcggcgcat
     4261 ctccgcaacg tgggcatcga ggaagcgggc cagctggacg gcctcctcgg tggacttgca
     4321 gacgaagcag gatgctctgg tcattcggtc tccgaggaca tatgttctga agccgccgga
     4381 ctcggccgcc gctctcgcac cccggtagag cgaggccgaa aggccgccct cggtatgcgc
     4441 cagcggcacg aagacctgat cctgattccg gctcgtttca atgacccggc cgaaaggctc
     4501 ctcgagctcg tagtcgccga gctcgatctc cagcgggccg accaccgaga ccgggatgac
     4561 cgcggcgccc gtcagcatct cctgggcgtt cacgaacgga gcaaggtcga ttcgcgtcac
     4621 gccgtcgctg agtccaagcg ctttctgacg cgcctcgatc gactcggggt cattcttacg
     4681 cggccaacgc ctcaactcca tgactcaaag cttacggcca agcgacgaac ccttcggaag
     4741 gtgttccttc cgacatttgg attgagacgg ccgcacgagc gaagcccgca cggcctctat
     4801 gcggcgccgc aaaggcggcg cctaggggga taatgccccc gcgatggcga cgggccagct
     4861 gatcgagatg aaggacgaag gccgcgaggc cttcgcagac gttctccgtt tctacgagct
     4921 tgcgaagcgc agggagtggc aggtgcgcga tctcccctgg ggtgagctcc cgccgattcc
     4981 ggaggcgaag ggctcgccgc aaagtcaggc tcggcgcagg gacatgtggc gctcggtcct
     5041 gacgcaacag ctccaggccg acatgcttgc gtgcgacatg gcgtcgcagc tgctcaacat
     5101 ggcgcccgac gtcgaggcga agctctacta ctcgacgatg gttcaggacg agtcgcgtca
     5161 caccgaggcc tggctcaagc tcgtgatcga ggccggcggc accgccgagc gcgatccgca
     5221 tctcgacaag ctcgcaaaga cgacgctcga agcggacacg ctcgaggaga aggtcttcct
     5281 gatgcaggtc ttctacgagc ggctgatcat ttcgaggttc cggctcatcg cccgctcttc
     5341 gcgcggcacc gtgctcgagg acctctgcgg ccgtctcgcc acggacgacg gcatccacca
     5401 cggcgccggc atggcctacg agcgcgtgct cctgcagaac ctctccaaga agatgaacca
     5461 gaagctcgtc gacgcggcca accggctgct gccagtgttc gccgcccacg cgctgtggcg
     5521 accgcgggaa cgcgcctgga tcgggcgcgc catggaggca cgcgacgtcg agcgcctccg
     5581 cgaggatctc gagctcggcg ttcgcctcgc cgagtcgctc ggcctggatg tcagcggcgt
     5641 cgagttgacg atcccgtaag gggcaccatg aacgcgacgc tgatggacgg cgccgcactc
     5701 gcggtgcggc tgcgggagga gatcaagcac gaggtcgccg agcttggcca cgtcggactg
     5761 gcgaccgtgc tcgtcggcga tgatccggcg tccgaggtct acatccgcct caagcacaag
     5821 gctgcggacg aagcaggcat cgaagcgatc gatcacaggc ttcctgccac aacgacggag
     5881 gacgagctcg tcgagctcgt ccagcatctc cacaaggacg acttcgtcga cgggattctg
     5941 gtccagacgc ccttgcccga ccagatcgac gaggcgcgcg tgatgctggc gatcgacccg
     6001 atgaaggacg tggacgggct ccatccgttc agcgccggcc agctctacct cggacggccc
     6061 accctcgtcc ccgccacgcc gctcggggtg atgcacctct tggacgagta caagattccg
     6121 atcgcgggct cgcgcgcggt ggtcgtcggc cgcagctcgc tcgtcggcaa gccgatggcg
     6181 ctgctgctct tgcatgcgaa cgcaaccgtc acggtctgtc attcccgcac ccaagatctc
     6241 gcccggcaca cgctggacgc cgatctcctc gtggccgccg tcggccttcc tgacgtcatc
     6301 accgccgaca tggtcaagca aggagcgacg gtgatcgatg tcggcatcac gcgtaccgaa
     6361 gctggactcg tgggggatgt cgaggaggac gtggccgagg tggccgcctt cctcacgccc
     6421 gtcccgggcg gcgtgggccc gatgacgatc gcggccttgc tcgggaacgc agtgcgggcg
     6481 gctcgctacc ggcgcgggaa gcttgcgttc ccccgcatct gagctaaact ccggcccgct
     6541 gtatcagtag tggcggctcg gtgcccgggc cgtcgcatgt tttgaaggag gaagtcgttg
     6601 gctcagggca ccgtgaagtg gttttcgaac gagaagggct atggattcat cgagcgtgag
     6661 ggtggcgagg atgtcttcgt ccatttctcc gccatcacgc aggaggggta caagtcgttg
     6721 accgagggtc agcgagttga gttcgaggtc gtgcagggcc ccaagggcgc tcaggccgcg
     6781 aacgtccagc ctgtctagct ttcctttcgg aaccaagtgt caagggcccc cggaaggggg
     6841 cccttttgtc ttagcaggag atgtctgctc gaatggagtc tgacccacac gctcgttgaa
     6901 acggccgacc ggacaaagcc cgatcggcct ctcgtcggcg ccgccggggc ggcgcctcag
     6961 ttagatcaga aggagaacat ggcgatctcg agagaagatg tcctgcacgt cgcggccctt
     7021 gcgcagctcg agctgagcga ggacgagatc gagcggctga tcgagcagct gaacgacatc
     7081 cttgccgccg tcgggaaggt gtcggagctc gatctctcgg atgtgccgcc gacgtctcat
     7141 cctctctcgg tcgtgaacgt cttccggccc gacgtgccac ggccttcgct gctcctcgac
     7201 gatgcgttcg ccaatgcccc ggagcgcgac cgcgacctct tccgcgtccc gccgacgggc
     7261 gccactgagg agccctcgtc gtgatcgaca cgcttcgact gactgccgag gatgcgaaga
     7321 gcctgcttga gcgcggtgaa gtctctggcg ccgagctgcg cgccgcctac cgtgaggcaa
     7381 tcgatgggcg caacgaggag ctcaactcct ttctgacact cgtcgacggc gacgacaacg
     7441 acggtgtgcc gatcgcgctc aaggacgtca tctcgacgaa aggtgtgcgc accactgcgg
     7501 gctcgaagat cctcgaggac tacgtgccgg tgttcgactc gaccgttgct gaccgctgca
     7561 aggcggccgg cctgcagctg ctcggcaaga cgaacacgga cgagttcgcc atgggctcgt
     7621 cgaccgagaa ctccgcctac gggccgtccc gcaatccgtg ggacccgacg cgcgtgcccg
     7681 gcggttcggg aggcggatcg gccgctgccg tgtcggcggg actggcaccg tgggcgctcg
     7741 gatcggatac gggcggctcg atcaagcagc cctcggctct ttgcggcaac gttggcctgc
     7801 gcccgacgta cggcaccgtg tcgcggtacg ggatcgtcgc gttcgcctcc agcctcgacc
     7861 aggtcggccc ggtgacgcgg accgtgcgcg acaacgccct tctctacggg atcatcagcg
     7921 gccgcgacga gtgcgactcg acgaccgtcg acgttccacc cgtcgagctg ccggaagcgg
     7981 aggacctgaa gggtctgcgc gtcggcgtcc cgaaggagct gaacgaggcc gacgggatct
     8041 cgcccggtgt cgcagaggca gtacggcgcg cgatcgacct ctgttccgag ctgggcgcgg
     8101 acgtgggcga atgcgagctc ccgcgctccg tcgagtacgg cctcgcctgt tactacctga
     8161 tcgctccggc ggaggcgtcc tcgaatctcg cgcgttacga cggcgtgcga tttgggcttc
     8221 gcgtccccgc ggagaactac tacgagatgg tcgcgcgcac gcgcgacgca ggcttcggcg
     8281 acgagccgaa gcgccggatc atgctcggga cctacgcgct ttccgccggc tactacgagg
     8341 cgtactacgg tcaggcgcag aaggttcgaa cggtgatcgc gcgcgagttc gccgaagcgt
     8401 tcgagcgctt cgacgtcctc gtcagtccga cttcgccgac cgttgccttc aagatcggcg
     8461 agaaagccga cagcccgctg gccatgtacc tcaccgacgt gctgacgatc cctccgaaca
     8521 tggccgggct gccggggctc tcgatcccat gtggcctgtc cgaaggcctg cccgtgggcc
     8581 tgcagctgat cggcgcgcag ttctcggaga acactctctt ccgcaccggc cacgcactcg
     8641 agcacgcgat cggtttcgac accgtcccag aacggctgcg atgacgaggc actgcttgca
     8701 gtgccgtcat caggaggcgt gggctctgcc cgggcctccg ccggcgccgc tggccgagac
     8761 cactcgaaca ggggcgcaag cgtgagaacc atcgtggacc gggccgccga cttcgtgctc
     8821 gcagtcgagc gggtgttcgg cacgcctccg cgagtgctgg atggctcgcg cgcagtgcag
     8881 atcgacgacg ttcgcctgtc gctcgaggcg ggcgaacgag agctctgcct catccgcatg
     8941 cacggcctgc tggaggaata cctcgcgatc ttcgaggtgc gtggcgacat cgaggtaccg
     9001 ctcctgcggg cgaaggagtt cctcgcgtga gcgaggaacg gggaagggtc gtgggaaacc
     9061 gggaggtttc ccatcgtctc atccgcgacg cacgcggtga tctcagcgcc gcgcgcgtcg
     9121 cggcgtt
//
LOCUS       DASVEU010000010         6390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1565524,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000010 DASVEU010000000
VERSION     DASVEU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565524"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="hemH"
                     /locus_tag="VF225_00465"
     CDS             complement(<1..756)
                     /gene="hemH"
                     /locus_tag="VF225_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7143755.1"
                     /translation="MNAAVVLMAYGSPERLADVPAYYADIRGGRPIPQELMEDLVERY
                     RRLGIEDGSPLNAITEETRTSLERALGDVPVFTGMKHWTPHIAEAVDAALVAGAETIV
                     GLVLAPHFSSMSIVGYRQQLERGLGGRAELAFIESWHDEPGFVKLLAERVRGTDAHVV
                     FTAHSLPARILESGDPYKHQLLETSQLVAERAALETWSFSFQSESQTGEPWLGPDILD
                     HLQELRGRGVRDVLVCPVGFVADHLEIRWDLDHE"
     gene            complement(753..1781)
                     /gene="hemE"
                     /locus_tag="VF225_00470"
     CDS             complement(753..1781)
                     /gene="hemE"
                     /locus_tag="VF225_00470"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7143756.1"
                     /translation="MEPLLVRACRREPVERTPVWFMRQAGRSLPEYRELRQRYGLFDI
                     VAEPELCAEVTLQPVRRHDVDAAVMFTDIMFPVLGMGVEVELVENVGPIISSPIRSRA
                     DVNRLVVPEAEESVPTILEAVRLVRAALRDDQAVIGFCGGPFTVAGYLIEGKPSREFA
                     TVKAMMYRDPETWHELMEKLADTFARYIAAKARAGADVIQVFDSWVGALSPSDYEEFV
                     APYSARILAAVEVPTIHFGTGTATLLSSMADAGGDVIGLDWRIPLDRGWAKVGEDRGV
                     QGNLDPAVLLGPWERIEFETRAILARAGGRPGHVFNLGHGVPPNADPAVLGRLTELVH
                     EATVEARV"
     gene            1848..2513
                     /locus_tag="VF225_00475"
     CDS             1848..2513
                     /locus_tag="VF225_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143757.1"
                     /translation="MRWLGYELEPPQAGALTRARVQLENAGTAPWRGLNLSYHWLDER
                     DNPILWDGLRYEVSAAPGERVERELELRGPIPPGPYHLAFDLVDERRFWLAELGNYSP
                     HLDVEVAPRDAAAARAHLPPNADLDPDWEERVYAAHMEGYAAVGGSIDARRPATELEP
                     YAPGGGRNPAFAHPLVLPSLLPPLVPNTEVAGLPAWQPEGDEPWLYDARIRLRLRSGR
                     RRG"
     gene            complement(2476..3276)
                     /locus_tag="VF225_00480"
     CDS             complement(2476..3276)
                     /locus_tag="VF225_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143758.1"
                     /translation="MTTVYGDLVRYRELFGNLFRRDVQAKYRGSLLGVAWTLANPVLL
                     MAVYLLVFSVLWKTQFGSEGHYALFLLVGLSAWIFFATSVQSASRSMLDNANLIRKTR
                     FPRQLLPLSVVATHLISFGVMLVALLVVNFALLPRVRATEWLALPLGLAIVAITCGLA
                     LAIASLNVLFRDIEFLVSALLLPLFFLTPVLYPLNDPQIARRDWVVDVIHWGNPLSPL
                     VEALRDPLFYGRLPGLGDTIYTVVAAALALALGAYVFNRVDDQIAVEV"
     gene            3342..5156
                     /locus_tag="VF225_00485"
     CDS             3342..5156
                     /locus_tag="VF225_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX7143759.1"
                     /translation="MKALAAIAAFPASAATIWLLLRTPLATRIAAAPREDRWHERTTP
                     MFGGIGIFAGLVTGVGVAVAVGAAPASRELLAILGGCTILFVAGLLDDVFSLGPIPKL
                     AAQVAAALLVIFNGLTISGLISNDLLAGIVALVWLVGLTNAFNLLDNMDGLAATLAGI
                     AATFFAVDAVTVHKSHAVLVLALALALACAGFLPFNLRLGKSAAVFMGDSGSQPLGFA
                     LGALGLTASWKVAGTTVATLLLPILVLAVPILDTTLVTIVRLLDGRPVYQGGRDHTSH
                     RLVYHGLSEKRAVVLLAVISAALGTTSLFYSVLDNAWVTLIGVLLTFALLVQFASGLS
                     DAERSPSFAADRGWLRTFVVNPRRLVESIVDFALIIASFAVAYYLRLQGSGSPYQRHI
                     FLVSIAVVLAVRYLAFIPFGLYRGVWRYAGARDAASIVAAVVVSEVVAYLILDATQTW
                     GPFPRSVFIIDALVCTILIGASRFWERAFVRGVSALTGRGDRNRTLIVGAGRGGRSLL
                     RELRETAGEQVVGFVDDDPRLSRRRLQGVPVLGGTGEIEGILSRARPDAVLVTIPDAP
                     RERLGLVIDACTLAQVPCRFVRRETDLDPRAVLGVTAD"
     gene            5164..>6390
                     /locus_tag="VF225_00490"
     CDS             5164..>6390
                     /locus_tag="VF225_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143760.1"
                     /translation="MAVVTARTTPAEQTLADRLLAAVPLLSIFLWLAIVYVIQAWAHK
                     TPWLFGDELELTQLSRAIADTGHAARRGDPYSFTTLWTYVLAPAWLIDNLHSAYATVK
                     YLTVIVMTATAFPAYALARLFVGRRPALFVAAASAAIPALAYSSMIVEEPFAYFWSAL
                     ALFLILRALITPSRWWIGGALVASLIAPLVRGELAMIPVVFLLAAVFLLWRSEYVNRW
                     RLSWSAWDWVGLVTLVVGAFVMISAVLGHRSLEWNYTTRLYKGRMLDLGLNAAGALTI
                     GLGVLPVVAGLAVLWRAPGEAARRELRIFRSVLLAAIVCFGLYTAVKSTYVSITFGTY
                     TYERNLIFLAPLLFTGTALWLERRRLHPIALALASVFVLILILTTPYEMGQEISYNAP
                     GLAVLQQANRYLSIDPT"
BASE COUNT         1002 a         2231 c         2073 g         1084 t
ORIGIN      
        1 ctcgtgatcg agatcccagc ggatctcgag atggtcggcg acgaagccga ccggacagac
       61 caggacgtcg cgaacccccc tgccgcgcag ctcctggagg tggtcgagga tgtcgggccc
      121 gagccacggc tcgcccgtct gggactcgct ctggaacgag aaggaccatg tctcgagcgc
      181 tgcgcgctct gcgaccagct gggaggtctc cagtagttgg tgcttgtagg ggtctcctga
      241 ctcgaggatg cgcgcaggca aggagtgggc tgtgaagacg acgtgcgcat ccgtgccgcg
      301 cacgcgctcc gccaacagct tgacgaaccc cggctcgtcg tgccagctct cgatgaacgc
      361 gagctcggcg cggcccccga gcccgcgttc caactgttgg cggtaaccca cgatggacat
      421 cgacgagaag tgcggcgcca gaaccaggcc gacgattgtc tcggcgcccg cgacgagagc
      481 ggcgtccacg gcctcggcga tgtgcggcgt ccaatgcttc atgcctgtga acaccggcac
      541 gtcgccgagc gcgcgctcga gtgacgtccg cgtctcctcc gtgatcgcgt tgagtggcga
      601 cccgtcctcg atcccgagcc tgcggtaccg ctcgacgagg tcctccatca gctcctgagg
      661 aatggggcgg ccgccgcgga tgtcggcgta gtacgcagga acgtcggcca gccgctccgg
      721 cgagccgtac gccatcagca cgaccgccgc gttcacacgc gcgcttcgac cgtcgcctcg
      781 tgcacgagct ccgtcagacg cccgagaaca gccgggtctg cattgggtgg cactccgtgc
      841 ccgaggttga atacatgacc cggccgccct ccggcacggg cgagaatagc gcgggtttca
      901 aactcgattc gctcccacgg tccgagcaga accgccggat cgaggttgcc ctgcacgccg
      961 cggtcctcgc ccaccttcgc ccatcctcga tcgagcggga tccgccaatc gagcccgatc
     1021 acgtcaccgc ctgcgtcggc catcgacgac agcaaggtcg ccgtgcccgt cccgaaatga
     1081 atcgtcggga cctcgaccgc ggcgaggatg cgggccgagt acggcgccac gaactcctcg
     1141 tagtccgacg gcgagagcgc gccgacccac gagtcgaaga cctggatgac gtcggcgccg
     1201 gctcgcgcct tcgcagcgat gtagcgcgcg aacgtatccg cgagcttttc catcagctcg
     1261 tgccacgtct cggggtctcg atacatcatc gccttgacgg ttgcgaactc gcgactcggc
     1321 ttgccctcga tcaggtatcc ggcgacggtg aacgggccgc cgcaaaaccc gatcaccgcc
     1381 tggtcgtcgc gcaacgctgc gcggacgagc cgaactgcct cgagaatcgt gggtacagac
     1441 tcctccgcct ccgggacgac cagacgattc acgtcggcac gggagcgaat cggcgacgag
     1501 atgatcgggc cgacgttctc cacgagctcg acctcgacgc ccatgccgag gacggggaac
     1561 atgatgtccg tgaacatcac ggcggcatcg acgtcgtgac ggcgaaccgg ctggagcgtc
     1621 acctcggcgc agagctcggg ctccgcaacg atgtcgaaga gcccgtaacg ctgccgcagc
     1681 tcgcggtact cggggagcga gcggcccgcc tggcgcatga accagacggg cgtgcgctcg
     1741 accggctcac gccggcacgc cctcacgaga agcggttcca cgccacgtag gctagtccgg
     1801 ctctggaagc agtcggcgac caccccattc cggcagggcc gctcgcggtg cggtggctcg
     1861 gctacgagct cgagccgccg caggcggggg cgctgactcg cgcccgcgtt cagctcgaaa
     1921 acgcgggcac cgctccgtgg cgcggcctca acctgtcgta tcactggctc gacgagcgcg
     1981 acaatccgat cctctgggac gggctccgct acgaagtgag cgcggctcca ggagagcgcg
     2041 tcgaacgaga gctcgagttg cgcggcccga tcccacccgg cccgtaccac ctcgccttcg
     2101 atctcgtcga cgagcggcgc ttctggctcg cggagctcgg caactactcc ccgcacctcg
     2161 acgtcgaggt tgccccccgc gacgccgcgg cggcacgcgc gcacctgccg ccgaacgccg
     2221 acctcgaccc cgactgggag gaacgcgtct acgcggcgca catggagggc tatgccgctg
     2281 tcggcggatc gatcgatgca cggcgcccgg ctacggagct cgaaccgtac gcgccgggcg
     2341 gaggacgcaa cccggcgttc gcgcatccgc ttgtcctacc ttccttgctg ccgccgctcg
     2401 tcccgaacac ggaggtcgcc ggcctgcccg cgtggcaacc ggaaggcgac gagccctggc
     2461 tctacgacgc ccggatcaga cttcgactgc gatctggtcg tcgacgcggt tgaagacgta
     2521 ggcgccgagc gcgagagcaa gggccgcggc gacgaccgtg tagatcgtgt cgccgaggcc
     2581 gggcaagcgg ccgtagaaaa gcggatcccg cagcgcctcg accaacggcg agagcggatt
     2641 cccccagtgg atgacgtcga cgacccagtc gcgccgcgca atctgcggat cgttgagcgg
     2701 atacagcacg ggagtcagga agaagagcgg caacaagagc gccgacacca ggaactcgat
     2761 gtcgcgaaac aacacgttca gcgacgcgat cgcgagggca agcccgcacg tgattgcgac
     2821 gatcgctagg ccaagtggca gagcaagcca ctcggtcgct cgaacgcgtg gcaggagcgc
     2881 gaagttcaca acgagcagcg cgacgagcat gacgccgaag ctgatcaggt gggtcgcgac
     2941 gaccgacagc ggcaggagct ggcggggaaa ccgtgtcttc cgaatgaggt tcgcgttgtc
     3001 cagcatcgac ctggacgcgg actgcaccga cgtggcgaag aagatccagg ccgagagccc
     3061 cacgagcagg aagagcgcat agtgcccctc tgaaccgaac tgagtcttcc agagaaccga
     3121 gaagacgagc agatagacgg ccatcaggag cacagggttc gcgagcgtcc acgccacgcc
     3181 gagaagcgag ccccggtact tcgcctgcac gtcgcggcga aagaggttcc cgaagagctc
     3241 gcggtagcgg acgagatccc cgtataccgt cgtcataggc gccgcctagt ctagaggtgg
     3301 cttcacatgg cacttacagc ccttcgttac gcttctgctc cgtgaaggcg ctcgcggcga
     3361 tcgctgcatt ccccgcctcg gccgcgacga tctggctgct ccttcgcacc ccgctggcaa
     3421 cgcgcatcgc tgcagcgccg cgtgaggacc gctggcacga gcgcaccacg cccatgttcg
     3481 gcgggatcgg gatcttcgcc ggcctcgtca ccggcgtcgg ggttgcggtc gccgtcggcg
     3541 cagccccggc cagcagggag ctcctcgcga tcctgggcgg ctgcacgatc ctcttcgtcg
     3601 ccgggctgct cgacgacgtc ttttcgctcg gaccgatccc gaagctcgcc gctcaggtcg
     3661 cggcggccct gctggtcatc ttcaacgggt tgacgatctc cgggctgatc tcgaacgacc
     3721 tcctcgccgg aatcgtcgca ctcgtatggc tcgtcgggct gacgaacgct ttcaatctgc
     3781 tcgacaacat ggacgggctt gcagcaacgc ttgccgggat cgcggcgacg ttcttcgccg
     3841 tcgacgccgt cactgtgcac aagagccacg cggtgctcgt gcttgcgctc gcgctcgcgc
     3901 ttgcgtgcgc gggctttctc cccttcaacc tgcggctggg caagtcggcg gcggttttca
     3961 tgggcgactc gggcagccag ccgctcggct tcgccctggg cgcactcggc ctgaccgcga
     4021 gctggaaggt ggccggcacg accgttgcaa ccctgctgct cccgatcctc gtgcttgccg
     4081 tcccgattct cgacacgacg ctcgtcacga tcgtgcgact gctcgacggg cgccccgttt
     4141 atcagggggg acgcgaccac acctcccacc ggctcgtgta tcacggtctc tcggagaagc
     4201 gcgccgtcgt cctgctcgcg gtgatctccg ccgcactcgg aacgaccagc ctcttctact
     4261 cggtgctgga caacgcctgg gtgacgctga tcggcgtgct actcacattc gcgctgctcg
     4321 tccagtttgc gagcgggctc tccgacgcag aacgctcgcc gagcttcgcc gccgaccgtg
     4381 gctggctacg taccttcgtc gtgaatccgc gccggctcgt cgagtcgatc gtcgacttcg
     4441 cactcatcat cgcctccttc gcggtcgcgt actacctccg gctccaggga agcggtagcc
     4501 cttaccagcg gcacatcttc ctcgtctcga tcgccgtggt gcttgctgtc cgctatctgg
     4561 cgttcatccc gttcggcctc taccgcggcg tctggcggta cgccggcgca cgtgacgcag
     4621 cgagcatcgt cgcggcggtc gtggtgtcgg aggtcgtggc gtacctgatt ctcgacgcga
     4681 cccagacctg gggcccgttc ccacgaagcg tgttcatcat cgacgcgctc gtctgcacga
     4741 ttctcatcgg cgcctcccgt ttctgggagc gggcgttcgt gcgtggagtc tccgcactca
     4801 ctgggcgcgg cgaccggaac cggacgctca tcgtcggcgc gggtcgaggc ggccgcagtc
     4861 tgctgcgcga gctacgcgag acggccggcg aacaggtggt cggtttcgtc gacgacgatc
     4921 cgcggctttc gcggcgacgg ctgcaagggg tgcccgttct cggcggcacc ggagagatcg
     4981 aagggattct ctcacgcgcg cgtccggacg ccgttctcgt cacgattccc gacgcgccgc
     5041 gcgagcggct cgggctggtc atcgacgcct gcacgctcgc acaggttcct tgccgcttcg
     5101 tccgtcgcga aaccgacctc gatcctcgcg ccgtcctcgg cgtaactgcc gactgattcg
     5161 cgaatggccg tcgtgaccgc gcgaacgacg ccggctgaac aaacgctggc agatcgcctg
     5221 ctggcggccg tgccgctgct gagcatcttc ctctggcttg cgattgtcta cgtgatccag
     5281 gcctgggcgc acaagacgcc gtggcttttc ggcgacgagc tcgagctcac tcagctctcg
     5341 cgagcgatcg ccgacaccgg ccatgcggct cggcgcggcg acccgtactc cttcacgacg
     5401 ctctggactt acgtgctcgc gccggcgtgg ctgatcgaca acctccactc cgcctacgcg
     5461 acggtgaagt acctgacggt gatcgtgatg accgccacgg cgtttccagc gtacgcactc
     5521 gcccgcctgt tcgtcggccg caggccggcc ctcttcgtcg ccgcagcgtc cgcggcgatt
     5581 cccgccctcg cgtactcgtc gatgatcgtc gaggagccgt tcgcgtactt ctggtcggct
     5641 ctggcgctct tcctgatcct gcgcgcgctg atcacaccat cgcgctggtg gatcggcggc
     5701 gcgctggtcg cgtcgttgat cgcgccgctc gtgcgcggcg agctcgccat gattccggtg
     5761 gtcttcctgc ttgccgcagt gttcctgctc tggcggtccg agtacgtcaa ccgatggcgc
     5821 ctgagctgga gcgcgtggga ctgggtgggc ctcgtgacgc tcgtcgtcgg ggccttcgtg
     5881 atgatcagtg cggtgctcgg gcatcgctcg ctcgagtgga actacacgac tcgcctctac
     5941 aaaggtcgga tgctcgacct cggcttaaac gccgcgggcg cgttgacgat cgggcttggc
     6001 gtcctgccgg tggtggctgg gctggcggtg ctctggcgtg caccaggcga ggcggcacgc
     6061 cgggagctgc ggatctttcg tagcgtcctg ctcgccgcga tcgtctgctt cggcctctac
     6121 accgcggtca agtcgaccta cgtctcgatc acgttcggga cgtacaccta cgagcgcaac
     6181 ctcatcttcc tcgcgcccct gctgttcacg ggaacggcgt tgtggctcga gcgccgtcgc
     6241 ctgcatccga tcgcactcgc actcgcgtcg gtcttcgtcc tgatcctgat cctcaccacg
     6301 ccctacgaga tggggcagga gatttcgtac aacgcgcccg gcctagcggt ccttcaacaa
     6361 gcgaaccgct atttgagcat cgatcccacc
//
LOCUS       DASVEU010000011        13939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_788764,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000011 DASVEU010000000
VERSION     DASVEU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13939
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788764"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2236)
                     /locus_tag="VF225_00495"
     CDS             complement(2..2236)
                     /locus_tag="VF225_00495"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX7143761.1"
                     /translation="MLKTVLLSGLASLVIAVDWLRFEEPRSGGGRPFVLAFLAIAPML
                     LRPVWLRLVGIAVSALLAAWVAFSISPLAARPGGDGFFGPLWSRFSRGFLDFYDYRLP
                     FDPAGRPGMHAVILIGIFGFTLAVALAIAARKALLAVVLFFVAAGWPATLLAGGNELG
                     RGLAILAVALALLAGMTERPSRLALIAAGAVIGGALALSSSPAVAKSAFLDWQHWDFY
                     TRPQKAVSVRYVWDAHYGGVSFPKKKTTVLTIRAPQRSYYWRATVLDRFDGTRWLEDV
                     WHESALQRHKLMPAQAGNRSNLVPQKVTVVALEDNHVIGATTPVSNDLGKAAAYEGQG
                     VSRVIGGLHRDQHYTVWSYAPRPTPEELVRVPPTYPRVLTRPGSELDIARGVTAPPFG
                     APDRDARLLKPLTGRLLPYAQLYERARQVAGETSSPYAAAVALEAWFRSTGGFTYSEQ
                     PGTTPGLPPLVGFVIDTKTGYCQHFAGAMAVMLRLLGIPARVAAGFLPGSYHGDFWQV
                     TDHEAHTWVEVWFRGYGWLPFDPTPGRGHLSGSYSSTSLGFSVASAAKLLAGIVKGGE
                     VFGGQTVEGIIAHDPGRRNPRSAGDLPLGIGSRVTPEPKKRAPSLLFFLFLLAAGAAA
                     AVVLLKLARRKLRYLTRDPRRTAVACARELADFVHDQRVPAGQAATFRELGGTVKDGL
                     GVDASSFAEAATAARYGPPEGARQAAGSARKELRELKRVLRRSLGKYDRVRGLLSVRS
                     LGLG"
     gene            complement(2229..3431)
                     /locus_tag="VF225_00500"
     CDS             complement(2229..3431)
                     /locus_tag="VF225_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7143762.1"
                     /translation="MLTRRGRVIFALGFGIYVAAWAFGSKPLYPVATGLLLVVGVAWV
                     WVRLSDRPFRVKRGWGDREHVEGDDVPVVAELDPTGNVLPASVTLSERVGRLGEQRHV
                     LRRHGRRLSVRYVLEKVPRGRYAFEDVHAELADPFGLEQAIVELPAPGALLVYPRLVR
                     LGPLFTESGTRSHDGQRLLLRRHSGFELHSVREYEQGESLRRVHWPSTARRGQLMVKE
                     LEDSPRDEIAVLLDADAKAVVGESFEMQVRAAGSILEAYVRRGRRGVLVLNSKRREVQ
                     HVHSPAADWRRALELLAAVEATGDTPVARLLAEEDSPAARALELVVVTSRLEPALIDR
                     LVQRALSRRKVSIVFVDPASFNGAPRRPEPALLRLQAAGIPLAVIRAGDDLAAGLSGE
                     PATASAHA"
     gene            complement(3434..4399)
                     /locus_tag="VF225_00505"
     CDS             complement(3434..4399)
                     /locus_tag="VF225_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7143763.1"
                     /translation="MSKPDSQRVESVPAAETIRAIVENLGRVVHAPEETLSLCVLCLV
                     GEGHLILEDFPGVGKTMLAKALARSLDVSFSRMQFTPDLLPSDVTGVNVFNQRSNEFE
                     FRPGPVFANLLLVDEINRASPKTQAALLECMQEQQVTTDGITNQLARPFMVMATQNPI
                     EYEGTYPLPEAQLDRFTMRIAIGYPPLSEEAKMLTEQTADPPLDGLEPVANAAEAIAL
                     ADEAKAIFVEESLNRYVVALLRATRGDSRLYLGASPRAGIATLRVAKARALADSRTYL
                     EPDDIKAVAVPVLAHRLILAPEARSTGVTGEQIVRETLDKTPVPV"
     gene            complement(4383..5546)
                     /gene="coaBC"
                     /locus_tag="VF225_00510"
     CDS             complement(4383..5546)
                     /gene="coaBC"
                     /locus_tag="VF225_00510"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7143764.1"
                     /translation="MARVLVGVSGGIAAYKACDLVRLFVKGGHEVVPLVTPGAERFVR
                     AETFFALARQSPSEDLYVHLTQADLLVVAPLTANTLAKLAHGLADNLVAEAALAHRGP
                     VLLAPAMNPRMWASPATMANVETLRARGVLFVGPDEGETAEGEWGMGRMAEPDEIFRR
                     SRELLGETDSLSGRRVLVSAGGTREPFDSVRFLGNRSSGRMGVALAEEARRRGAEVTL
                     LAANLAVPPPRGIEVVETPTAAAMLDEALARRDADVVLMAAAVADYRPAESAVGKRPK
                     DDEAWQVTLEPTADVLRTLGEQRTNGQVLIGFAADGGDRGLARAREKLMQKHVDLVVY
                     NDVSRNDIGFDAGDNEVVLVSAAEERRVAKAPKDQIAATIVDAAEELLDEQAR"
     gene            complement(5598..5831)
                     /gene="rpoZ"
                     /locus_tag="VF225_00515"
     CDS             complement(5598..5831)
                     /gene="rpoZ"
                     /locus_tag="VF225_00515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7143765.1"
                     /translation="MIHPRIDELLENVDSRYALVIVAAKRARQINDYHHQLGEGIGFD
                     QAPPPLVESRSKNYLTMALEEVAEGKLKYKYPK"
     gene            complement(5828..6454)
                     /gene="gmk"
                     /locus_tag="VF225_00520"
     CDS             complement(5828..6454)
                     /gene="gmk"
                     /locus_tag="VF225_00520"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7143766.1"
                     /translation="MTNRAERSHHDGEGARALVLVVTGTSGAGKGTLEKILLERMPEV
                     ELAVSATTRGQRPGELNGREYWFITPEKFERLVEDGEFLEYVDLPWGARSGTLRSEID
                     RIQANGRVPLLDLETDGALRVQETIPGAVTIFVDAPTFDELERRLRERATESSGEIQV
                     RLDLARKQQTLKDRFDYAIVNDDVERAAHELTEIVRRELQTAGTMSRP"
     gene            complement(6514..6843)
                     /gene="mihF"
                     /locus_tag="VF225_00525"
     CDS             complement(6514..6843)
                     /gene="mihF"
                     /locus_tag="VF225_00525"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX7143767.1"
                     /translation="MVSSKTQAEAPIRSLDQRMEALKRANDIRVRRARLKKDLKDGRV
                     QIEQILSNPPEYVSTAKVFDMLMAVPKFGRVKAARFLNMCRISQSKTVGGLSDRQRSE
                     LIGLFHR"
     gene            complement(6919..7800)
                     /locus_tag="VF225_00530"
     CDS             complement(6919..7800)
                     /locus_tag="VF225_00530"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX7143768.1"
                     /translation="MLLNASGCLDALTAPDVARSLDAFVTKTVTPLPRDGNTPVRIAE
                     TDVGMLNSIGLANPGIDRFITRNIPQLAELGVPVWVSVGGFETDDYADICSRLDDHAE
                     VSVIELNVSCPNVEAPAESAAEIVGASRLATRKPLWAKLSPAVPDIAEVARAAQAAGA
                     DGLSLVNTIRGLKLDSTTLRPVLGPGQGGLSGPALKPIALAAVATCYRATQMPIVGMG
                     GVQTGLDALELIAAGAQHVALGTVLFADPDAPARVRAELHAAAAARGWKTYEDAYAIA
                     HDETLSARATLDERSSV"
     gene            complement(7790..8449)
                     /locus_tag="VF225_00535"
     CDS             complement(7790..8449)
                     /locus_tag="VF225_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143769.1"
                     /translation="MRRERLSVVANEQVGPYTLLRVARGGVEPGVPGQFFMLEAPGRL
                     LPRPMSLCLALRGELAFLIDAIGPGTAALCELDRGDAIHVLGPLGNGFDLDLERPLLV
                     GGGIGIAPLPYLSEALGRPPAVLGFRSDRHAEAAELVPNAQACVEPTLVTELIPAGMD
                     VLACGPEPMLQAVRTLAPAAQLAWEAPMACGYGACYGCAVEIDGELKLLCVEGPVLRA
                     A"
     gene            complement(8430..11489)
                     /gene="carB"
                     /locus_tag="VF225_00540"
     CDS             complement(8430..11489)
                     /gene="carB"
                     /locus_tag="VF225_00540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7143770.1"
                     /translation="MPRRHDLESICLIGSGPIVIGQACEFDYAGCQALKVLREDGFRT
                     IVVNSNPATIMTDPGFADRTYLEPLDLEGVADVLGRERPDALLPTMGGQTALNLAREL
                     AEAGVLADLGVELIGAPLDVIRRAEDRELFRDAVHSVGLRTPKSRIVTEVSQLAGLAT
                     PAVVRPAFTLGGHGGGFVETVDQLYRQVELGIFESPIGQVLVEESVRGWDEFELEVVR
                     DRKDNVVIVCSIENIDPMGIHTGDSVTVAPQMTLSDHAYQELRDAAAAVVRAVGVECG
                     GSNIQFARERDTGELRVIEMNPRVSRSSALASKATGYPIAKVAAKLAVGYTLDEIPND
                     LTGTTPASFEPTLDYVVVKFPRFGFEKFPGADATLGTQMKSVGEAMGIGRTFVEAFRK
                     ARRSRELDGAEIWRLAESAHPWFAGELAGELEELRLEDLVADDFLRIKRAGFSDADIA
                     SKCGVAEEDVRASRRAWGVRPSYRRVDSCAGEVEAASTYYYSTWGEDDEAPPHGEKPR
                     VVILGSGPNRIGQGIEFDYCCVHAAQSFRALGYEAVMVNCNPETVSTDYDTSDRLYFE
                     PLGLEEVLAVCERERPDGVAIQFGGQTPLKLARGLEAAGHRIIGTAFEAVDVAEDRER
                     FGALLDRLGIRCPEWGIARSGDEARGIAERIGFPVLIRPSYVLGGRAMRICYSVDDLG
                     TIDSPVLIDRFIESAIEIDVDAVSDGDDTYIGAVMQHVEEAGVHSGDSACVLPAPSLS
                     AAQDASIRQIVRRLARALGVVGLMNVQLAVVDESIYVLEANPRASRTVPFASKATGVN
                     LVEAACRVMAGARLSALDLFESHSLGHVSVKAAVLPFARFPGADPVLGPEMRATGEVM
                     ASAADLPTAFAKAERAAGRPLPVTGTAFLSVCDADKRAVVQVARGLAELGFKLLATAG
                     TADRLAAAGLEVDRVRKVTEEGEGPTVVDLIRRGRCNLVINTPQGSGARTDGYLIREA
                     ALVARVPCITTLSGATAAVDAIANARSEESLSLQELQERIEHETRAA"
     gene            complement(11473..12516)
                     /gene="carA"
                     /locus_tag="VF225_00545"
     CDS             complement(11473..12516)
                     /gene="carA"
                     /locus_tag="VF225_00545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7143771.1"
                     /translation="MTGFLALEDGTVFRGDSVGAEGFAVGEAVFTTAMTGYQEVVTDP
                     SFTEQLVCFTAPMIGNYGVAEGRSESARPHARAVLMREARGPAWTDWLHERGIVALSG
                     VDTRSLVLKLRDAGALRAIAVAGEGSAEQAVSVARSQPSMAGRALAAEVSTPRPYIYS
                     DAGRVRIAVVDYGCKRSILRRVAGAGAAVTVFPHDATADELLRFDAVLLSNGPGDPEP
                     LEDEIAVVRELIGRAPLLGICLGHQLLALAAGHETFKLPFGHRGANHPVLERASGRVL
                     VTSQNHGFAVAPSDSREATYVSLYDGTVEGLDFPLEHARSVQFHPEAGPGPHDAWPII
                     EQWVEEVAYAQAA"
     gene            complement(12513..13814)
                     /locus_tag="VF225_00550"
     CDS             complement(12513..13814)
                     /locus_tag="VF225_00550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7143772.1"
                     /translation="MLFAKDTPVENIVVRGARVVDPVEAVDAVLDVRVDQGVIVALGQ
                     DLDTNSHRVIDAEGLVLAPAFVDPHVHLRTPGREDEETIASGTAAAAAGGYCTILAMP
                     NTDPVVDSAATLGSLIEAARTEAEIPVGFLAAITKGQSGAELTEMGELAAAGAVGFSD
                     DGVPVAAPGLMRRALQYGAVTGKRIALHCEERTLSREGQMHEGEVSAELGFTGYPSVA
                     ESLMVERDLALAAYERQPIHLLHLSAWESVQALRRARGDGVQATAEVTPHHLCLTDEA
                     IRSLDPNLKMNPPLRSASDRTALIEGLKDGTIVAIATDHAPHARHEKDVPFEAAPFGV
                     TGLETAFAALYTQLVEPGLVPLEVVLERMSAGPARAYGLEEPSIAVGSTANLVLLDLN
                     ASYRVTEEMFRSRSVNSWLLGETVNGKVRATIAAGRLAYAS"
BASE COUNT         2280 a         4816 c         4655 g         2188 t
ORIGIN      
        1 cttagccgag cccgagcgaa cggacggaca gaagaccccg gacacggtcg tacttgccga
       61 gacttcggcg gagaacgcgc ttcagctcac gaagctcctt gcgcgcggag cccgccgcct
      121 gccgcgcacc ttccggtggc ccgtaacgcg cagcggtcgc tgcctccgca aagctgctcg
      181 catccacgcc gagcccgtcc ttgacggtgc cgcccagctc ccggaacgtc gccgcctgtc
      241 ccgccggcac gcgctggtcg tggacgaaat ccgcgagctc gcgcgcgcac gctactgcgg
      301 ttcggcgcgg atcgcgtgtc aggtagcgga gcttgcgtcg cgcgagcttc aggagaacga
      361 cggccgcggc ggctcccgcg gcgaggagga acaggaagaa caacaggctc ggcgccctct
      421 tcttcggctc gggcgtgaca cggctgccga tgccgagggg aaggtcgccg gccgagcgcg
      481 gattccggcg gccagggtcg tgcgcgatga ttccctcaac ggtttggccg ccgaacacct
      541 cgccgccttt gacgattccg gccagcagct tcgcggcact cgcaacgctg aagccgagcg
      601 acgtcgagct gtagctcccg ctcagatgcc cacgccccgg cgtcgggtcg aacggaagcc
      661 agccgtaccc gcggaaccac acttcgaccc aggtatgcgc ctcgtggtcg gtgacctgcc
      721 agaaatcacc gtggtagctc ccggggagaa aacccgccgc cactcgagcc ggaatgccga
      781 gcaaccgcag catcaccgcc atcgcaccag cgaaatgctg gcagtacccg gtcttcgtgt
      841 cgatcacgaa gccgacgagc ggcggcagtc ccggagtcgt gcccggctgc tcggagtacg
      901 tgaagcctcc ggtcgaccgg aaccacgcct cgagcgcgac ggccgccgcg tagggcgagc
      961 tcgtctcgcc ggccacctgc cgggcgcgtt cgtacagttg cgcgtacggg agcagccggc
     1021 cggtcagggg tttcaacagg cgcgcgtcgc ggtcgggagc accgaacggc ggcgcggtca
     1081 cgccacgggc gatgtcgagt tcactgccgg gcctcgtgag cactcttgga tacgtaggcg
     1141 gaacccgcac gagctcctcg ggcgtcggcc gcggcgcgta gctccagacg gtgtagtgct
     1201 gatcgcggtg cagaccgccg atcacccggg acacaccttg cccttcgtag gccgcggcct
     1261 tacccaggtc gttcgagacc ggggtggttg ccccgatcac atggttgtct tcgagcgcga
     1321 cgacggtgac tttctggggg acgaggttcg agcggtttcc cgcttgggcc ggcatcagct
     1381 tgtggcgctg caacgcggac tcgtgccaca cgtcttccag ccaacgcgtg ccgtcgaatc
     1441 gatcgagcac cgtcgcacgc cagtaatacg accgctgtgg ggcgcggatc gtcagtaccg
     1501 tcgtcttctt cttcgggaag ctgacgccgc cgtaatgcgc gtcccagaca tagcggacgc
     1561 tcacggcctt ttgtggacgc gtatagaagt cccagtgctg ccagtcgagg aacgcgctct
     1621 tcgcgacggc cggcgagctc gagagagcca gcgccccgcc gatcacggcg cctgcggcga
     1681 tcagcgccag ccggctcggt cgctcggtca tgcctgcaag cagtgcgagt gcgaccgcga
     1741 ggatggcgag cccgcggccg agctcgttgc cgccggcaag caacgtcgca ggccaccccg
     1801 cggcgacgaa gaagagcacc acggccagca gcgccttgcg ggcggcgatt gcgagcgcga
     1861 cggcgagcgt gaacccgaag atcccgatca ggatgaccgc gtgcattccc ggacggcccg
     1921 ccgggtcgaa gggaagccgg tagtcgtaga agtccaggaa gccccgcgaa aaccgggacc
     1981 agagcgggcc gaagaaaccg tcaccgccgg gccgagccgc caacggcgag atggaaaacg
     2041 caacccaggc ggcgagcagc gcggacaccg cgattcccac caggcgcagc cagaccggac
     2101 gcagcaacat gggtgcgatc gcgaggaaag cgagcacgaa cgggcggccc ccacccgacc
     2161 ggggttcctc gaaccgaagc cagtccacgg cgatgacgag cgaagccagc ccggagagca
     2221 gaaccgtctt aagcatgtgc gctcgccgtc gcaggctcgc cggacaggcc ggccgccagg
     2281 tcgtcgccgg cgcggatcac ggcgagaggg atccccgcgg cctggaggcg cagcagcgcc
     2341 ggctcggggc gccgtggcgc accgttgaag ctcgcgggat ccacaaagac gatcgacacc
     2401 ttgcgccggg aaagtgcgcg ctggaccagc cggtcgatga gtgcgggctc cagcctcgac
     2461 gtcacgacga ccagctcgag ggcccgcgcg gcgggactgt cctcttctgc gagcagccgc
     2521 gcaaccggag tgtctccggt cgcctcgacg gccgcgagca actcgagcgc acgacgccag
     2581 tccgccgcag gcgagtgcac gtgctggacc tcgcgccgct tcgagttgag caccagcact
     2641 cctcgacgac cgcgccgcac gtacgcctcg aggatcgacc cggcggcgcg gacctgcatc
     2701 tcgaagctct cccccaccac ggccttggcg tcggcgtcga gcaggacggc gatctcgtcg
     2761 cggggcgagt cctcgagctc cttgaccatc agttgaccgc gacgcgcagt cgaaggccag
     2821 tggactcggc gcaaggattc gccctgctcg tactcgcgca cgctgtgcag ctcgaagccg
     2881 ctgtggcggc ggagcagcag gcgttggccg tcatgtgagc gtgttccgga ttcggtgaag
     2941 agcggtccga ggcggacgag ccgcggatac acgagcagcg cccccggtgc gggtagctcc
     3001 acgatcgcct gctcgaggcc aaaaggatcc gcgagctcgg cgtgcacgtc ctcgaatgcg
     3061 tagcggccgc gaggaacctt ctcgaggacg tagcgaacgc tcaaccgccg cccgtgccgc
     3121 ctgaggacgt gccgctgctc gccaagtcgg ccgacccgct cgctgagcgt cacggacgca
     3181 ggcagaacgt ttccggttgg atcgagctcg gccacgacgg gaacgtcgtc accctcgacg
     3241 tgctcgcgat cgccccaccc tcgcttgacc cggaacggcc ggtcggagag gcgcacccag
     3301 acccaggcga cgccgacgac cagaagcaga ccggtcgcga ccgggtagag aggcttggag
     3361 ccgaaggccc aggcggcgac atagatcccg aagccgagcg cgaagatcac ccgaccgcgc
     3421 cgagtcagca ttactagacc ggtactgggg tcttgtcgag cgtctcccgc acgatctgct
     3481 cgccggtaac cccggtcgag cgcgcctccg gagccaggat cagccggtgc gcgagcacgg
     3541 ggaccgccac tgccttgatg tcgtccggct cgaggtaggt ccggctgtcg gcgagcgcgc
     3601 gtgccttcgc aactctcagg gttgcaatgc ccgcgcgagg gctcgcgccc aggtagagcc
     3661 gcgaatcgcc acgagtcgcg cggagaaggg ccaccacata gcggttgagg ctctcctcga
     3721 cgaagatcgc cttcgcctca tccgccagcg cgatcgcttc cgctgcgttc gccacgggct
     3781 cgagcccgtc gagcggagga tccgcggtct gctccgtgag catcttcgct tcctccgaca
     3841 gcggcgggta gccgatggcg atccgcatcg tgaaccggtc tagctgcgcc tccggcaacg
     3901 gatacgtccc ctcgtactcg atcgggttct gcgtcgccat caccatgaac ggccgggcca
     3961 gctggttcgt gattccgtcc gtcgtcacct gctgttcctg catgcactcc agcagggctg
     4021 cttgcgtttt cggtgacgct cggttgatct cgtcgacgag caacaggttg gcgaacacgg
     4081 gccccggccg gaactcgaac tcattcgaac gctggttgaa gacattcacg ccggtgacat
     4141 ccgacggcaa caggtcgggc gtgaactgca tccgtgagaa agacacgtcg agcgagcgcg
     4201 cgagcgcctt ggcgagcatc gtcttcccca caccgggaaa gtcctcgagg atgagatggc
     4261 cttcgccgac gaggcagaga acgcagaggc tgagcgtctc ctcgggcgcg tgcacgaccc
     4321 gaccaaggtt ctcgacgatt gcgcggatcg tctccgccgc cgggacgctc tccacccttt
     4381 ggctatcggg cttgctcatc taacagctcc tcggctgcat ctacgatcgt cgccgcgatc
     4441 tggtccttgg gagccttggc gacccgccgc tcttctgcgg cactgacgag cacgacttcg
     4501 ttgtcgccgg cgtcgaagcc gatgtcgttc cgcgacacgt cgttgtagac cacgaggtcg
     4561 acgtgcttct gcatcagttt ctcgcgcgcg cgcgcgaggc cgcggtctcc gccgtcggcc
     4621 gcgaagccga tcagcacctg gccgttcgtt cgctgctccc cgagcgtgcg caagacgtcg
     4681 gcggtgggct cgagcgtcac ctgccacgct tcgtcgtcct tcgggcgctt gccgactgcc
     4741 gactctgcgg gccggtagtc ggcgacggcc gctgccatca gcaccacgtc tgcgtcacgc
     4801 cgtgccagtg cttcgtcgag catcgctgcg gccgtggggg tctccacgac ctcgatgcca
     4861 cgcggtggcg ggacggcgag attcgcggcg agcagagtga cctcggctcc tcgtctgcgt
     4921 gcttcctccg cgagggctac gcccatccgg ccggatgagc gattaccgag gaaacggacc
     4981 gagtcgaagg gttcccgcgt tccgcctgca ctgacgagga cgcggcggcc ggagagcgaa
     5041 tcggtttcgc cgagcagctc gcggctacgc cggaagatct cgtcaggctc cgccatcctg
     5101 cccatacccc attcgccttc ggctgtttcc ccttcatcag gcccgacgaa gagaacaccg
     5161 cgcgcccgaa gcgtctcgac gttcgccatg gttgccgggc tcgcccacat gcgcggattc
     5221 atcgccgggg cgagcaggac cgggccgcgg tgagccaggg cggcctcggc gacgagattg
     5281 tccgcaagcc cgtgcgccag cttggcgagc gtgttcgccg tgagcggcgc aacgaccagc
     5341 aggtcggcct gggtcaggtg aacgtagagg tcttcggacg gtgactgccg agcgagagcg
     5401 aagaaggttt ccgccctgac gaagcgctcc gcgccggggg ttaccaatgg gacgacctcg
     5461 tgaccgcctt tgacgaacag gcgaaccagg tcgcaagcct tgtaggcggc aattccaccg
     5521 ctgacgccga ccaggacccg agccatgccg caattctgcc ctttcggcag agaggcgacc
     5581 aaaaacctgg gagagcgcta cttcgggtac ttgtacttga gcttgccctc ggcgacttcc
     5641 tcgagggcca tggtcaggta gttcttcgag cgcgattcca ccaggggcgg cggagcctgg
     5701 tcgaagccga tgccctcacc gagctggtgg tggtagtcgt tgatctggcg agccctcttg
     5761 gcggcgacga tgacgagcgc atagcgcgag tcgacgtttt cgaggagctc atcgatgcgg
     5821 ggatggatca tggacggctc atggtacctg cggtctggag ttcccgacgg acgatctccg
     5881 tcagctcgtg cgccgctcgc tccacgtcgt cgttgacgat cgcgtaatcg aaccggtctt
     5941 tgagcgtttg ttgcttgcga gcgaggtcga gccgaacctg gatctcgccg gagctctcgg
     6001 tcgctcgctc gcgtagacga cgctcgagct cgtcgaatgt cggtgcgtcg acgaagatcg
     6061 tgaccgctcc gggaatcgtc tcctgcacgc gtagcgcccc gtcggtttcg aggtcgagca
     6121 gcggcaccct tccgttcgcc tggatgcggt cgatctcgga gcgcagcgtg cccgaccgcg
     6181 caccccacgg gaggtcgacg tactcgagga actctccgtc ctccacgagc cgctcgaact
     6241 tttcgggcgt gatgaaccag tactcccgtc cgttcagctc gcccggtcgc tgcccacggg
     6301 tggtcgcgga aacagccagc tcgacttcgg gcatccgctc gagcaggatc ttctcgagcg
     6361 tgcctttgcc ggcacccgag gtgccggtaa cgacgagaac cagcgctcgc gccccctctc
     6421 cgtcgtggtg gcttcgctcg gctcgattgg tcatttcaga ggtaggcatc ggctgctccc
     6481 gcgtctgcgc acgggcagcg cggcacgtag tgcctaacgg tggaagaggc caatcagctc
     6541 agaccgctgc cggtcggaga ggcctcccac cgtcttcgac tgactgatcc ggcacatgtt
     6601 caggaaccgt gccgccttca cccgtccgaa cttcgggacc gccatcagca tgtcgaacac
     6661 cttcgcggtc gacacgtatt ccggagggtt gctgaggatc tgctcgatct ggacgcgtcc
     6721 gtccttgaga tccttcttca gcctggctcg tctgacacgg atgtcattcg ctctcttcaa
     6781 cgcctccatg cgttggtcga gcgaacgaat cggtgcctcg gcctgggtct tggacgacac
     6841 catgagcgcg ggagtctaac aagcgacctg cgacggtcaa gcggctatgt ttgcggttct
     6901 gagcaggctt tttgaggctt agacactcga cctttcatcg agagttgccc gggcggacaa
     6961 cgtctcgtca tgagcgatcg catatgcatc ttcgtatgtt ttccatccac gcgcggctgc
     7021 cgccgcgtgc aattccgcgc gcacgcgtgc aggcgcgtcc ggatcggcga agagcaccgt
     7081 tccgagcgcc acgtgttgcg cacccgcagc gatgagctcg agcgcgtcga ggcctgtctg
     7141 caccccgccc ataccgacga tcggcatctg cgtcgcccga tagcacgtcg cgacggcggc
     7201 gagtgcaatc ggcttgagcg ccggccccga gaggcctcct tggccggggc cgaggacggg
     7261 ccggagtgtc gtgctgtcga gtttcagccc acggatcgtg ttgacgagtg aaaggccgtc
     7321 ggcgccggcc gcctgcgcgg cgcgcgcgac ctccgcgatg tcaggcacgg ccggggagag
     7381 cttcgcccac aacggcttgc gcgtcgccag ccgcgacgcg ccgacgatct cggccgcgct
     7441 ctcggccggc gcctcgacgt tggggcacga cacgttcaac tcgatcacgc tcacctccgc
     7501 gtgatcgtcc agccgagagc agatgtcggc gtagtcgtca gtctcgaatc cgccgaccga
     7561 cacccagacg ggcacgccga gctccgcgag ttgcgggatg ttgcgcgtga tgaagcggtc
     7621 gatgccggga ttcgcgagac cgatggagtt gagcatcccg acgtcggttt cggcgatacg
     7681 caccggtgtg ttcccgtcgc gcggcagcgg cgtgaccgtc ttcgtcacga acgcgtcgag
     7741 gctccgcgcg acgtcaggcg cagtgagcgc atcgagacag ccggaggcgt taagcagcac
     7801 gtagcaccgg cccctccacg cacaggagct tgagctcgcc gtcgatctcg accgcgcagc
     7861 cgtagcaggc gccgtaccca cacgccatcg gcgcctccca cgccagctgt gccgcggggg
     7921 caagcgtgcg caccgcctgc agcatcggct cgggcccgca ggcaaggacg tccatccctg
     7981 ccgggatcag ctccgtcacg agggtcggct ccacgcaggc ctgggcgttc ggtacgagct
     8041 ccgcagcctc ggcgtgccgg tccgagcgga aaccgagcac cgcgggcggc cgcccaagcg
     8101 cttccgatag gtagggcagg ggagcgatcc cgatcccccc acccacgagc agcgggcgct
     8161 ccaggtcgag gtcgaagccg ttcccgagcg gaccgagcac gtggatggcg tcgccgcggt
     8221 cgagctcgca gagcgccgcg gttccgggcc cgatcgcatc gatgaggaaa gcgagctccc
     8281 cgcgaagcgc gaggcagagg ctcatcggcc tcggcagcag gcgcccgggt gcctcgagca
     8341 tgaagaactg tccgggcacg ccaggctcca cgccgccgcg cgcgacacgc agcagcgtgt
     8401 acgggccgac ctgctcgttc gcgacgacgc taagccgctc gcgtctcatg ctcgattcgt
     8461 tcctgaagct cttgcagcga aagcgactct tcgctgcggg cgttggcaat cgcatcgacc
     8521 gcagcggtcg caccggacag tgtcgtgatg catggcacgc gtgccacgag cgcggcctcc
     8581 cggatgagat agccgtcggt ccgcgcacca gatccctgcg gggtgttgat gacgaggttg
     8641 caacggcctc gccggatgag atccacgacc gtcgggccct cgccttcttc cgtcaccttg
     8701 cgcacccggt ccacctcgag ccctgccgcg gcaagcctgt ccgcggtgcc ggcggtggcc
     8761 agcaacttga acccgagctc ggccagcccg cgcgcgacct ggacgaccgc ccgcttgtct
     8821 gcgtcgcaca ccgacaggaa cgcggtaccg gtgacgggca gcggccggcc cgctgcccgt
     8881 tccgccttcg cgaaggcggt cgggagatcg gcggcgctcg ccatcacctc tcctgtcgcg
     8941 cgcatctcgg ggccgaggac cgggtcggca ccggggaagc gcgcgaacgg aagcaccgca
     9001 gctttcaccg agacgtgccc aagggagtgt gactcgaaca ggtccagcgc ggagagacgt
     9061 gcgcccgcca tgacgcggca cgccgcctcg acgaggttca ccccggtcgc cttcgaggca
     9121 aaggggaccg tgcgcgacgc gcgcgggttg gcctcgagca cgtagatgct ctcgtcgacg
     9181 accgcgagct gcacgttcat gagaccgacc accccgagcg cacgtgcgag cctgcgcacg
     9241 atctgacgga ttgaggcgtc ctgcgccgcg ctgagggaag gcgcgggcaa cacgcacgcg
     9301 gagtccccgg agtgcacgcc cgcctcttcg acgtgctgca tgaccgcgcc gatatacgtg
     9361 tcgtctccgt cggagacagc gtcgacgtca atctcgatcg cgctctcgat gaagcggtcg
     9421 atcaggacgg ggctgtcgat cgtgccgagg tcgtccaccg aatagcagat gcgcatcgcc
     9481 cgcccgccga gcacgtacga agggcggatc agaaccggaa aaccgatccg ctccgcgatc
     9541 ccgcgcgcct cgtcgccgct ccgcgctatc ccccactccg gacagcgaat cccgagccgg
     9601 tcgagcaggg cgccgaagcg ctcgcgatcc tctgcgacgt ccacggcctc gaacgccgtc
     9661 ccgatgatgc ggtgcccggc cgcctcgagc ccacgcgcca gtttcagcgg ggtctgaccg
     9721 ccaaactgga tcgcgacgcc gtccggccgc tcccgctcgc acaccgcgag cacttcctcc
     9781 agcccgagcg gctcgaagta gagcctgtcc gaggtgtcgt agtccgtgga cacggtctcc
     9841 ggattgcagt tgaccatcac ggcttcgtag ccgagcgcgc ggaagctctg cgcggcgtgc
     9901 acgcagcagt agtcgaactc gattccctga ccgatgcggt tcgggccgga gccgaggatg
     9961 acgacacgtg gtttctcgcc gtggggcggc gcctcgtcgt cctcgcccca ggtcgagtag
    10021 taatacgtcg aggccgcctc gacctccccg gcgcaggagt cgacccgacg gtaggaagga
    10081 cgtacgcccc acgcccggcg gcttgcgcgg acgtcttcct cggcgacgcc gcacttcgag
    10141 gcgatgtccg cgtcggagaa ccccgcgcgc ttgatccgca gaaagtcgtc cgcaaccagg
    10201 tcctccagcc tcagttcctc gagctcgccc gcgagctcgc ccgcaaacca gggatgtgcg
    10261 ctctcggcga gccgccagat ctccgccccg tccagctcgc gcgagcgacg cgccttgcgg
    10321 aacgcctcga cgaaggtgcg cccgatgccc atcgcttcac cgaccgactt catctgcgtt
    10381 ccgagcgtcg cgtcggcgcc gggaaacttc tcgaacccaa aacgcggaaa cttgacgacg
    10441 acatagtcga gcgtcggctc aaaactcgcg ggtgtggtac cggtgagatc gttcgggatc
    10501 tcgtcgagcg tgtagccgac cgcgagcttc gcagcgacct tggcgatcgg gtagccggtc
    10561 gccttcgagg cgagcgcgga ggaacgggag acgcgaggat tcatctcgat cacgcgcagc
    10621 tctcccgtgt cacgctcgcg cgcgaactgg atgttcgatc cgccgcactc gacgccgacc
    10681 gcccgcacga ccgccgccgc cgcgtcgcgg agctcctgat acgcgtggtc cgacagcgtc
    10741 atctgcggcg cgaccgtcac ggagtccccg gtgtggatgc ccatcggatc gatgttctcg
    10801 atcgagcaga cgatcacgac gttgtccttc cggtcgcgca cgacctcgag ctcgaactcg
    10861 tcccagccgc ggaccgattc ctcgacaagc acctgtccga tcgggctctc gaagatgccg
    10921 agctcgacct ggcggtacag ctggtcgacc gtctcgacga agccgccacc gtggccgccg
    10981 agcgtaaatg ccggtctcac gaccgcgggg gtcgcgagac cggccagctg cgagacctcg
    11041 gtgacgattc gtgatttcgg tgtgcgcagg ccgaccgagt ggacggcgtc acggaagagc
    11101 tcgcggtcct ccgcacgtcg gatgacgtcg agcggggcgc cgatcagctc gacgccgaga
    11161 tcggctagga caccggcctc cgccagttcc ctcgcgagat tcagcgcagt ctggccgccc
    11221 atcgtcggca gcagcgcgtc gggccgctcg cggcccagca cgtcggcgac accctcgagg
    11281 tcgagcggtt cgaggtaggt ccgatctgcg aaaccggggt cggtcatgat cgttgcgggg
    11341 ttcgagttca cgacgatcgt ccgaaagccg tcctcgcgca gaaccttcaa cgcctggcag
    11401 cctgcatagt cgaattcgca cgcctgcccg atcacgatcg ggccggagcc gatcaggcag
    11461 atcgactcga gatcatgccg cctgggcata cgccacctcc tcgacccact gttcgatgat
    11521 cggccaagcg tcgtgcgggc cgggaccagc ttccggatgg aactgcaccg accgagcgtg
    11581 ttcgagcgga aagtcgagcc cttcgacggt tccgtcgtaa agcgagacgt acgtcgcctc
    11641 gcgtgagtcc gatggcgcga ccgcgaagcc gtggttctgg ctcgtgacga ggacgcgtcc
    11701 cgacgcgcgt tcgagaactg gatggttcgc gccgcggtgc ccgaagggaa gcttgaaggt
    11761 ctcgtgcccg gcggcgaggg cgagcaactg gtggccgagg cagatcccga gcagcggcgc
    11821 gcgaccgatc aactcgcgca cgacggcgat ctcgtcctcg aggggctcgg ggtcgcccgg
    11881 accattcgag aggagcacag cgtcgaaacg gagcagctcg tctgcggtcg cgtcgtgcgg
    11941 aaacactgtc accgccgcac ccgcgcccgc cacgcgacgg aggatggagc gcttgcagcc
    12001 gtaatcgacg acggcgatcc gtacgcgccc ggcgtctgag tagatgtagg gcctcggagt
    12061 cgagacctcg gctgcgagcg cgcgacccgc catcgacggc tgcgacctcg cgaccgaaac
    12121 ggcctgctcg gcagagccct caccggctac cgcgatcgca cggagcgcac ctgcgtcgcg
    12181 gagcttcaga acgagggagc gggtgtcgac tccgctgagc gcaacgatgc cgcgctcgtg
    12241 cagccagtcc gtccatgcgg ggccgcgggc ttcgcgcatc agaactgcgc gggcatgtgg
    12301 ccgggcggat tcggatctgc cctccgccac gccgtagttc ccgatcatcg gcgctgtgaa
    12361 gcacacgagc tgctcggtga agctcggatc ggtgacgacc tcctggtaac cggtcatcgc
    12421 ggtggtgaac acggcctcgc cgaccgcgaa gccctcagct ccgacggagt cgccgcgaaa
    12481 gaccgtgccg tcctcgagcg cgaggaagcc cgtcatgatg cgtacgccaa ccgtccggcg
    12541 gcgatcgtcg ctcgtacctt gccgttcacc gtctcgccga gaagccagga gttcacggaa
    12601 cgcgaccgga acatctcctc cgtgactcgg tagctcgcgt tcaggtcgag caggacgaga
    12661 ttcgctgtgg agccgacggc gatgctgggt tcctcgagcc cgtacgcgcg tgcggggccg
    12721 gcggacatcc gctcgagcac cacctcgagc ggaacgaggc cgggctcgac cagttgggtg
    12781 tagagcgccg cgaacgccgt ctcgaggccc gtaacgccga atggggcggc ctcgaacggc
    12841 acatccttct cgtgccgcgc atgcggcgcg tggtcggttg cgatcgcgac gatcgttccg
    12901 tccttcagcc cttcgatcag tgccgtgcga tccgaggccg accgcagcgg cgggttcatc
    12961 ttcaggttcg ggtcgagcga ccggatcgct tcgtcggtca ggcagagatg gtgcggagtt
    13021 acctccgcag ttgcctgcac gccgtctccg cgggcacgcc ggagcgcctg cacggactcc
    13081 cacgcagaca ggtgaagcag gtggataggc tgtcgctcgt acgcggctag cgcgaggtca
    13141 cgttcgacca tcaggctctc ggccaccgag ggatagccgg tgaaaccgag ctccgccgaa
    13201 acctcgccct catgcatctg gccctcgcgt gagagcgttc tctcctcgca gtgcagcgcg
    13261 atccgtttgc cggtcacggc gccgtactgg agtgcgcgcc gcatcagccc gggcgcagca
    13321 acgggaacgc cgtcgtcgct gaaaccgacc gcgcctgctg cggcgagctc gcccatctcg
    13381 gtcagctcgg ctccggactg gcctttcgtg atcgccgcca aaaatccgac gggaatctcg
    13441 gcctcggtgc gcgcagcctc gatcaacgag cctagagtcg ccgcggagtc gacgaccggg
    13501 tcggtgtttg gcatcgcgag gatcgtgcag tagccgccgg cggcggcggc cgccgtgccc
    13561 gaggcgatcg tctcctcgtc ctcccggccg ggtgtgcgga ggtgcacatg ggggtcgacg
    13621 aaggccggtg caagcaccag accttcggcg tcgatgacgc ggtgcgagtt cgtgtcgagg
    13681 tcctggccga gtgccacaat cacgccttgg tccacgcgca cgtcgagcac ggcatcgacg
    13741 gcttcgaccg ggtcgacgac acgagcgccg cgcacgacga tgttctccac gggagtgtcc
    13801 ttggcgaaca gcatcacgcg acctccagca ccgattcggt cttgacttcg atcgggccgt
    13861 tcgtcagcag gtcgtagagg actgccatcc gcgtcacgag accggccctc acctgcgtct
    13921 cgatcagcgc gtccgcgtg
//
LOCUS       DASVEU010000012         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1566765,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000012 DASVEU010000000
VERSION     DASVEU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566765"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..278)
                     /locus_tag="VF225_00555"
     tRNA            complement(189..278)
                     /locus_tag="VF225_00555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Ser,seq:gga)
     gene            complement(365..688)
                     /locus_tag="VF225_00560"
     CDS             complement(365..688)
                     /locus_tag="VF225_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143773.1"
                     /translation="MLKRFALLLSVAMLAAFAVTAAPVSANGGSDAVRGARCVVAGVK
                     FLASNGLLVKAALRQVDYDTIDSDGGGDAGLINADLPTPAFLPLGEVIRLHFTNPELF
                     DWCNT"
     gene            complement(705..1238)
                     /locus_tag="VF225_00565"
     CDS             complement(705..1238)
                     /locus_tag="VF225_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX7143774.1"
                     /translation="MQKRLVVLLATLVGVASLAVAAQAAPSGQAASGKTIVTVAAGDK
                     RFTTLVALVKQAGLVGTLNGKGPFTVFAPTNAAFTKLKQSSPDTFEAVTTDKALLKTV
                     LTYHVLGKRVPSMAAIAAAKKNASVKTVQGESIKLSLKGGKLTLNGSSKVIVADVKAS
                     NGVVHAIDAVLVPPSAA"
     gene            1390..1971
                     /locus_tag="VF225_00570"
     CDS             1390..1971
                     /locus_tag="VF225_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7143775.1"
                     /translation="MAYVGEDVELVRRVAEGDGDALRELYGLYGKVVFGMAYRVLGDR
                     QLAEDCTQDVFVALWRGARSYDPKRARVTTWLFTVTRNTAIDIVRRREVRRADPLPED
                     ESAWAKDTAPDPAEVAATAEQAERIASALAELPRPQLEAVSLAYFDELSHAEIAKRLD
                     LPLGTVKGRIRLGLERLRELAPSYALKLEAAGE"
BASE COUNT          351 a          653 c          714 g          313 t
ORIGIN      
        1 gatcgacacg gcagccgagc ggtgcgccaa ggcgaagacg acgacgaagg gcaagggcaa
       61 gggcaagggc aacgggcacg ccaagcagta cgcggtctgc ctgcagcgaa ccgtgtagtc
      121 ggtctccgac ttgaagtcgg ataccgaggg gcatgcgcgg gggaaccatc ggttcccccg
      181 cgctcgtacg gagagggcgg gatttgaacc cgcgacgcag cgattaacca cgtacgcgat
      241 ttccagtcgc gctccttcgg ccgctcggac acctctccga gggcattcac cttagcgccg
      301 caccaacgag ctgtggccgc ccggaggcgg ccacagcttg ggggaactca ggccgatcgg
      361 gggactaggt gttgcaccag tcgaagagct ccgggttcgt gaagtgaagc cggatcacct
      421 ctccgagcgg caggaacgcc ggcgtgggga ggtcggcgtt gatgaggccg gcgtcaccgc
      481 cgccgtccga gtcgatcgtg tcgtagtcga cctgacgcag tgcggccttg acgagcaggc
      541 cgttcgacgc gaggaacttg acgcctgcga caacgcagcg agcgccgcgg acggcatccg
      601 agccgccgtt ggcgcttacc ggcgcggcgg tcaccgcgaa cgcggcaagc atcgcgacgg
      661 agagcaggag tgcgaatcgc ttcagcatcg ggagctcctt ccgcttacgc cgcgctcggc
      721 ggcacgagaa ccgcgtcgat tgcgtggacg acgccgttcg acgccttgac gtccgcgacg
      781 atcaccttcg aggagccgtt gagcgtcagc ttgccgccct tgagggagag cttgatcgac
      841 tcaccctgaa cggtcttcac cgacgcgttc ttcttcgcgg cggcgatcgc cgccatgctc
      901 ggcacgcgct tccctagcac gtggtaggtg agcacggtct tgagcagcgc cttgtcggtc
      961 gtcactgcct cgaacgtgtc cgggctgctc tgcttcagct tcgtgaacgc ggcgttcgtc
     1021 ggcgcgaaga ccgtgaacgg ccccttgccg ttgagcgtcc cgacgaggcc cgcctgcttg
     1081 acgagcgcaa cgagcgtcgt gaaccgcttg tcgcctgcgg cgacggtcac gatcgtcttg
     1141 ccagacgctg cctggccgct cggcgcggcc tgtgcggcga cggcaagcga ggcgacgccg
     1201 acgagcgtcg cgagtaggac tacgagtcgc ttctgcatta cgagcacctc ctgaatcggg
     1261 ttgaaagcct gtcggaggtg ctacggggga acggggcctc gtagatcaat cgcgagccac
     1321 gatgatcgga atcggcgtgc gcgacgaata catcgacgag atgcaaaaaa atcgtgcgat
     1381 agttgcggca tggcgtacgt cggggaggac gtcgagctcg ttcgtcgggt cgcagagggc
     1441 gacggcgacg cgctgcgcga gctgtacggc ctttacggga aggtcgtctt cggcatggcc
     1501 taccgcgtgc tcggcgatcg tcagctcgcc gaggactgca cgcaggacgt ctttgtcgcg
     1561 ctgtggcgtg gcgcgaggtc gtacgacccg aaacgagcgc gagtgaccac ctggcttttc
     1621 accgtcacac ggaacacggc catcgacatc gtgcgccggc gcgaggtgcg ccgtgccgat
     1681 ccgctccccg aggacgagag cgcctgggcg aaggacacgg cgccggaccc agcggaggtc
     1741 gccgcaaccg ccgagcaggc cgaacggatc gcctccgcgc tcgccgagct tccgcggccg
     1801 cagctcgagg ccgtctcgct cgcgtacttc gacgagctct cgcatgccga gatcgcgaag
     1861 cgcctcgacc tcccgctggg caccgtcaag gggagaatcc gcctgggact cgagcggctc
     1921 cgagagctcg cgccgtccta cgctctgaag ctggaggcgg ccggtgaata aggacgagcg
     1981 gctcgagctc gcagcgacgg aagcactcgg ccgggcgacg gcggaggagc t
//
LOCUS       DASVEU010000013         4633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_13073,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000013 DASVEU010000000
VERSION     DASVEU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13073"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF225_00575"
     CDS             <1..198
                     /locus_tag="VF225_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143776.1"
                     /translation="TNVYCIGPEYDRAVRSWRRKCTMLGFLYLAIPIFGAALCGALDK
                     ASRRGDHEHDKSPVVRLCRGI"
     gene            complement(752..1909)
                     /locus_tag="VF225_00580"
     CDS             complement(752..1909)
                     /locus_tag="VF225_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7143777.1"
                     /translation="MADLSDARALRELVAAQESDTVERKRTADSTTLAKVVSAFANTR
                     GGWLLLGVDDDGTVPGWRPKGTAHPRDWLRDVLENVLDPLPHFEAELFEMDGITIGVV
                     RVPRSSAKPHFIDRTGEVFERRNGQTRRLNGRQVREMTQQGTAGMDAALARFDHRERA
                     LDVAMTLDAPRESTAMHARAFASIVRISVAEPSDVLREWVHSTEALSRSHAFVWSAAR
                     ALNDRDWCTPGEPPPPRTTAGGHAATSEWDSRILKEAKVAWDRAGLGGVRFAGERPDD
                     SGIFYLLSGQARDHWLVIALEYLFGCLANAAVFGPAAVRWDLYGIRGADVTTVRDRNV
                     VAAQGVIPAHYNNMIAIDTEVDLGTTSADGAATKLWQRLERLSGAQHYSGC"
     gene            complement(2003..2206)
                     /locus_tag="VF225_00585"
     CDS             complement(2003..2206)
                     /locus_tag="VF225_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143778.1"
                     /translation="MPERTNLSHPCEEPDVAQRVTTPSGEREQDLPLDRMGRKGSARS
                     RLAYSATTGDFVGVGSYGTAGQS"
     gene            2354..3520
                     /locus_tag="VF225_00590"
     CDS             2354..3520
                     /locus_tag="VF225_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143779.1"
                     /translation="MALIAPEFDIERSALLRAVIDDTGVQVWDFRTDTGVDGRVFADE
                     TDPALREEVEERIDEVLAGSTEVDLAAFARDDPFFSIGHIRPGELTEDPAAEARRLVA
                     LRVIELGLARSMARLERDAFGPPQRDARAFSVVPGLWDALNDEGLLPLDADRAPEVPM
                     SAEGYVNFGDHVLFPHPDLRHSRELWWDLCELAREGLAVRVAIDPHRVVVRDELPMKL
                     IEDYWFGVKVTRDNLDSLADEDLGWAIHYRNPDSELGRRLEIFYPLLATEFRWSRGPD
                     ALKLLDVEEIVPPESSQTRTDDIVRNRYLHSHRDATRRAFVHANGALRVYDAEKYTPS
                     REAPLAGRDRLTEQRKLWHVHGELTDEKWSRLLTGWFRQNELVIEYFGELLDER"
     gene            complement(3527..4306)
                     /locus_tag="VF225_00595"
     CDS             complement(3527..4306)
                     /locus_tag="VF225_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143780.1"
                     /translation="MRERRNARAEAVYAKTGADELRVAAQQGPLTIDVLGENEPYTKE
                     HFTYVLDRLEELLEDVLSPSSETYPLFDDATGAVLQRMIAGEAHADPEWTQARHIGLA
                     SELVLHLEAFPRASMDVILDVRGQLQKPLRRFRAAVAEFAHQLEYVPVDKTFRVEVRA
                     LYVRVLEPELHDIRESIENLGVRPTLLRGAAPVSGAAVALGIGARLGMPDLALMGLMA
                     AGAGATVAAEVKHGIDEDRARRKNRCFWLYEADRRLRRATR"
BASE COUNT          705 a         1549 c         1613 g          766 t
ORIGIN      
        1 accaacgtct actgcatcgg ccccgaatat gatcgggccg tacgcagttg gcgccggaaa
       61 tgcaccatgt tgggattcct ctatctggcg atcccgatct tcggcgctgc gctctgcggt
      121 gcgctggaca aggcgtcgcg acggggagat catgagcacg ataagtctcc cgtcgtcaga
      181 ctttgccgcg gaatctgagc tgggtcgagc ggcgatgttg atgtccgggg tggccccgag
      241 caagcgccgc gatcgtgggg gcgacgcgtt cgaccgtggc gcgcctgggc tcgatcgcgc
      301 gcaggcggac ttgcttcgtc agcagcgggc ccggtgcgga gacaagatcc tcgtgctgcg
      361 gcggcgcgcg tgattgctga cgggcccgac ccgcccgcgc cgcctccgcc gccacggccg
      421 ccacgagacc cgggcgttga ggaaaagcgg gaccctaggc ggccgtccga ctacgagcgc
      481 ggtccaggca agcccaagcc acagcggccg tcagcggcga gctggcaagg gcgccagcaa
      541 acccccggca tgccgaccgg tcgcccgtcc gtcgccgtat gtcgcgcggg agatgcccgg
      601 tagcccgtcc ctgagcgggg cccgaacgag gggtcccgcg cctcgttgca gggcggctgg
      661 ctcgtcccac acccgaacgc ctaccggcgc gaccggggaa ggcggatggg ccggggtctc
      721 gcgctcgccg ccggtgctca ctcacctccc tctagcagcc cgagtagtgc tgtgcgcccg
      781 acagtcgctc gagccgctgc cagagcttcg tcgccgcgcc gtcggcggag gtcgtgccga
      841 gatcgacctc ggtgtcgatc gcgatcatgt tgttgtagtg cgccgggata acgccctgag
      901 cggcaacgac atttcggtcg cgcacggtcg tcacgtccgc gccgcggatg ccgtacaggt
      961 cccaccgaac agccgcaggc ccgaacactg cagcgttggc gagacatccg aagaggtact
     1021 caagtgcgat gacaagccag tgatcacggg cctggccaga gagcaagtag aagatcccgc
     1081 tgtcgtccgg tcgctcgcca gcgaaccgca cgcctccgag gccggcacga tcccaagcca
     1141 ccttggcctc tttgaggatc cggctgtccc actccgacgt cgctgcgtga cctcccgcgg
     1201 tcgttcgcgg gggcggcggt tcgcctggtg tacaccagtc tcgatcgttc agggcacgcg
     1261 ctgctgacca gacgaacgca tggcttcggc ttagggcttc cgttgaatgg acccattcac
     1321 gaagtacgtc cgacggctcc gcgacgctga ttcggacgat cgacgcaaac gcgcgcgcat
     1381 gcatcgccgt cgactcgcga ggggcgtcga gcgtcatcgc gacatccagc gcacgctcgc
     1441 gatggtcgaa ccgcgccaag gccgcgtcca tgcccgctgt gccttgctgc gtcatctctc
     1501 gaacttgcct gccgttcagg cggcgcgttt gcccattgcg ccgctcgaaa acctcgcccg
     1561 tgcggtcgat gaagtgcggc ttcgcggaag accgcggcac gcgcacgact ccaatggtga
     1621 tcccatccat ctcgaacagc tcggcctcaa agtgagggag cgggtcgagg acgttctcta
     1681 ggacgtcgcg gagccaatcg cggggatggg cggttccctt cgggcgccat cctgggacag
     1741 tcccgtcgtc gtcgacgccg aggagaagcc agccgcccct cgtgttggca aatgctgaca
     1801 cgactttcgc aagggtcgtg gagtcggctg tgcgctttcg ttcgactgtg tcgctttctt
     1861 gcgcagccac tagctcgcga agcgcgcggg catcgctgag gtcggccatc gtcactggca
     1921 aggaaggcac ccttcctatc ttgcgcgccg atcgcgtcag acgccggccg tccgcgtatc
     1981 gcaggacgag atcgatgccg tgctagctct ggcccgcggt gccgtaggac ccgacgccga
     2041 cgaagtcgcc ggtggtcgct gaatacgcca ggcgtgaacg cgcggagcct ttccggccca
     2101 tgcgatccag gggcaggtct tgctcgcgct caccggaggg cgtcgtgacc cgctgtgcca
     2161 cgtcgggctc ctcgcaagga tggctgaggt ttgtgcgttc aggcatcagc cgacggagcg
     2221 tacgttcgcc tgcggacggt cttggccgcg cgggtgcgcg ccggttgggc gggaagaacg
     2281 gatggcgtac attcggcggg cgggtgccga ctgctgcgct ctccaaggat tccccctccg
     2341 tacgctgtgg gacgtggccc tcatcgctcc ggagttcgac atcgagcgtt cggcgctact
     2401 ccgcgccgtg atcgacgaca cgggagtgca ggtctgggac ttccgtacag acacgggagt
     2461 tgacgggcgc gtgttcgccg acgagaccga cccggctctc cgcgaggagg tcgaagagcg
     2521 tattgacgag gttctcgccg gttcgaccga ggtggatctt gcagcgttcg cgcgcgacga
     2581 tccgttcttc tcgatcggtc acatcaggcc cggcgagctg accgaggacc ccgcggccga
     2641 agcgcgccgg ctggtcgcgc tgcgggtaat cgagctcggc cttgcgcgtt cgatggcgcg
     2701 gctcgagcgt gacgcgttcg ggccgccgca gcgcgatgcc cgcgcgttct cggtcgtacc
     2761 cgggctgtgg gacgcgctca atgacgaggg gttgcttccg ctcgacgccg atcgcgcgcc
     2821 ggaggtgccg atgagcgccg agggctacgt taacttcggc gatcacgtcc tcttcccgca
     2881 ccccgatctc cgccacagcc gcgagttgtg gtgggacctg tgtgagctgg cccgcgaagg
     2941 gctggcggtt cgcgtcgcga tcgatccgca ccgggtcgtc gtgcgcgacg agctccctat
     3001 gaagctgatc gaggactact ggttcggtgt caaggtcacg cgcgacaacc tcgactcgct
     3061 cgctgacgag gatctcggat gggctattca ctaccgcaac cccgactcag agctcgggcg
     3121 gcggctggaa atcttctacc ccctgctcgc caccgagttc cgctggtcgc gggggccgga
     3181 cgcgctgaag ctgctcgacg tcgaggagat cgtgccgccc gagtcgtcgc agacccgaac
     3241 ggacgacatc gtccgcaacc ggtatctcca cagccaccgc gacgcgacgc gacgcgcgtt
     3301 cgtccacgcg aacggcgcgc tccgcgtcta cgacgcggag aagtacacgc cgtcgcgcga
     3361 ggcgccactg gcggggcggg accgtctgac ggagcagcgc aagctctggc acgtccacgg
     3421 cgagctgacc gacgagaagt ggtcccggct gctgaccggc tggttccgcc agaacgagct
     3481 ggtcatcgag tacttcggcg agctgctcga cgagcgctga aagggcttac cgcgtcgcgc
     3541 gccgaagccg ccggtcggcc tcgtagagcc agaagcagcg gttcttgcgc cgggcacggt
     3601 cctcgtcgat gccgtgtttg acctcagccg cgaccgtcgc gccggcgccg gccgccatca
     3661 accccatcag ggcgaggtcc ggcatgccaa gtctggcgcc gatgccgagc gcgacggcgg
     3721 cgccgctgac tggagcagcg cctcgcagga gcgtcggccg gacgccgagg ttctcgatgc
     3781 tctcccggat atcgtgcagc tcgggctcga ggacacggac gtacagggcc cgcacctcga
     3841 cacggaacgt cttgtcgacg ggcacgtact cgagctggtg agcgaactcc gcgacggcgg
     3901 cccggaagcg gcgcagcggc ttctgaagtt ggccgcgcac atcgaggatc acgtccatcg
     3961 acgcgcgcgg gaacgcctcg aggtggagga cgagctcgct ggccaggccg atgtgccgcg
     4021 cctgagtcca ctccgggtcc gcgtgggcct caccggcgat catccgctga aggacggcgc
     4081 cggtcgcgtc atcgaacagc gggtacgtct cggagctcgg gctgagcacg tcctccaaga
     4141 gctcctccag acgatcgagt acgtacgtga agtgctcctt cgtgtacggc tcgttctcgc
     4201 cgagcacgtc gatcgtgagc ggcccctgct gtgccgcgac acgaagctcg tcggctccgg
     4261 tcttggcata cacagcctcg gctcgtgcgt tacggcgctc ccgcacccac cgcaggtcgg
     4321 acgtgaagcg gtcgacgagc gcctgctcgt gcggctgcag ttcgactccc gcgagctggc
     4381 ggtcatggtg gtacagcgcc gcctgccagc cgggcgtgtc gccgaggccg gaggcgcgga
     4441 aggcggcgac ttcctcggca ctcgccgggg ctgttgccgc gagcgtgtcg atggtacgcg
     4501 ttcaggttcc cggttgggtt tgcaggactt tcgggggatc gaggcgtagt tatggagcgt
     4561 ggagctgcgg cgagaggacg cgaagcggat cgcgcgcgag ggcggggatt cggcctgcga
     4621 gttgctgctg acg
//
LOCUS       DASVEU010000014         3980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1567874,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000014 DASVEU010000000
VERSION     DASVEU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567874"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF225_00600"
     CDS             <1..663
                     /locus_tag="VF225_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7143781.1"
                     /translation="LSVLIVDDQALVRAGFRMILDAEADIEVAGEAADGADAVAEALR
                     LKPDVVLMDVRMPQVDGIEATRRLLSADGVDTKVVMLTTFDMDEYVYDALRAGASGFL
                     LKDVPPEQLVAGIRAVAQGDALLAPSVTRRVIEEFVRRPPQSVRTLPPEVAELTTREL
                     EVLKLVARGLSNAEIAKELFVSDTTVKTHVAHVLTKLDVRDRVQAVVLAYECGLTGND
                     QA"
     gene            807..1379
                     /locus_tag="VF225_00605"
     CDS             807..1379
                     /locus_tag="VF225_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7143782.1"
                     /translation="MIRTGDTIHNPVTGERITFHATSADTNGESVVIECTVQPDGFVA
                     ATHVHPFQSERFAVIEGTLGLKSGKEKLTLGRGEIAVVEPGTPHRFWNAGEEEVRFVC
                     EVRPALQFESLLETMFALAEDGKTNRKGMPNPLRLAVIARAHFATVQLPHPPEWLQKA
                     GLAVAAPLGRALGYGPTYTPEVTAPAIPAV"
     gene            complement(1545..2267)
                     /locus_tag="VF225_00610"
     CDS             complement(1545..2267)
                     /locus_tag="VF225_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7143783.1"
                     /translation="MSDTVVFIPAWNEEENLPAVLDDLRRELPSADVLVVDDGSTDRT
                     AEIARGRGAEVLSLGSNRGLPIGIAAGYRWALDHGYAYCGRVDADGQHPPSELGRLLA
                     IVREGGCDVAVGSRFVSGDGYEPYRYLPSPARRVGTGLLRRGMKLALRRPFADATSGL
                     YAVNAKALPVLAEPFRTEAPEVEALLRLSDAGLRVEEVPVRMEPRAGGVSKLRGRKAV
                     KVTATVIGTLVAARFLRARRSD"
     gene            complement(2264..2776)
                     /locus_tag="VF225_00615"
     CDS             complement(2264..2776)
                     /locus_tag="VF225_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7143784.1"
                     /translation="MTPPAFVVLGSSRRDRAGAYRISRGCRRAVAQAAQLAERLEPRV
                     VVFSGWAPSGGASEAEQMQKLWSGPEVELVLEPTASTTAENAARTLPLLLERQIDHAV
                     VICTPPHVYRARWFFRRLYTAHGIRTSFEAPRVVPTPSAVIWELGALTVRSRQLRAAE
                     EELERGRSQP"
     gene            2872..3258
                     /gene="mscL"
                     /locus_tag="VF225_00620"
     CDS             2872..3258
                     /gene="mscL"
                     /locus_tag="VF225_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7143785.1"
                     /translation="MLKEFRDFLLRGNIVELAVAFVMGLAFAAVVNSLVNDLIMPVIA
                     MIIGKPNFSDLTFTINDAKFRYGAFITNVIQFVAIAGSVFFFVVKPVNALLTRFRSPA
                     EEGMPDEERRHQELLAALRGSAATNQ"
     gene            complement(3252..3617)
                     /locus_tag="VF225_00625"
     CDS             complement(3252..3617)
                     /locus_tag="VF225_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143786.1"
                     /translation="MPGRWRDVAGLIAAATVLAFVVAEPIIAATPRLVVHPVSVPRGA
                     TVTVSGKGCRAGDVVYLISPPFAGHAFVQHSVATRARSSGTFSRLVAIRTTIRPGRYL
                     ITARCGGGNLGVSAALRVY"
     gene            3691..>3980
                     /locus_tag="VF225_00630"
     CDS             3691..>3980
                     /locus_tag="VF225_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7143787.1"
                     /translation="MTVCPSCGSENPEGFRFCGSCGAALAEAPSAREVRKTVTILFCD
                     VSGSTALGERLDSESLRRVMERYFTLARNVLERHGGTVEKFIGDAVMAVFGIP"
BASE COUNT          664 a         1341 c         1335 g          640 t
ORIGIN      
        1 ctgagcgtcc tcatcgtcga cgatcaggcg ctcgtacgcg cgggctttcg catgatcctc
       61 gacgccgaag cggacatcga ggttgccggc gaggctgcgg acggcgcgga cgccgtagct
      121 gaggcgctgc gcctgaaacc ggatgtcgtc ctcatggacg tccgcatgcc gcaggtcgac
      181 gggatcgagg ccacacgccg cctgctctcg gccgacggcg tggacacgaa ggtcgtgatg
      241 ctgacgactt tcgacatgga cgagtacgtc tacgacgctc tcagggccgg cgcgagcggt
      301 ttcctgctca aggacgtccc gcccgagcag ctcgttgctg gcatccgcgc ggtcgcacag
      361 ggcgacgcac tccttgcgcc gtccgtgacg cgtcgagtca tcgaggagtt cgtccgccgg
      421 ccgccgcaat cggttcgaac cttgcctccc gaggtcgcgg agctgacgac gcgcgagctc
      481 gaggtcctga agctcgtggc acgaggcctt tccaatgccg agatcgcgaa ggagctcttc
      541 gtcagcgaca cgacggtgaa gacccacgtg gcgcacgtct tgacgaagct cgacgtccgc
      601 gaccgggtgc aggcggtcgt gcttgcctac gagtgcggcc tcaccggcaa cgaccaggcg
      661 tagacgacct catccgcaag agtgacaacg gcgtcctgaa agatccgccg caggacgacg
      721 cgagatcgtt ctccccgccg acgaaactgc ggccgcgcgg acctagcctc cggagtgtca
      781 atcgacagct acgaaggagg cacgagatga tccgcacggg cgacacgatc cacaaccccg
      841 tcacagggga gcgcatcacg ttccacgcca cgtcggccga cacgaacggc gaatccgtcg
      901 tgatcgagtg cacggttcag ccggacggat tcgtcgccgc cacgcacgtc catcccttcc
      961 agagcgagcg cttcgccgtg atcgagggaa cgctcggcct caagagcggc aaggaaaagc
     1021 tcacgctcgg tcgcggcgag atcgccgtcg tcgaaccggg aacgccccat cgcttctgga
     1081 acgccggcga ggaggaagtg cgtttcgtct gcgaggtccg acccgcgctg cagttcgagt
     1141 cgctgctcga gacgatgttc gcgctcgccg aagacggcaa gacgaatcgc aagggcatgc
     1201 cgaatccgct gcggctcgcc gtgatcgccc gggcgcactt cgccacggtg cagttgccgc
     1261 atccgcccga gtggttgcag aaggcgggtc tcgcagtggc cgctccgctc ggacgagctc
     1321 tcggctacgg cccgacgtac acgcctgaag tcaccgcacc ggccatcccc gcagtctgat
     1381 gaggaagaag cacttctact tcatgctgct ggtgctggca ctgatcttgc tctcgcttcc
     1441 tgggttcgcc gccaaggccg tgaagaaggc ggccgggccg gctctccggt tcgcctagct
     1501 cgacaccgtg ccccgtccgg cagcgggcgg ggcaccatgg tgggtcagtc cgatcgccgc
     1561 gccctgagaa atcgcgctgc gaccagggtg ccgatcacgg tggcggtcac tttgacggcc
     1621 ttccggccgc ggagcttcga gacgccgccc gcacgtggtt ccatcctcac tggcacttcc
     1681 tcgacacgca ggccggcgtc gctgaggcgg aggagggcct cgacctcagg cgcctccgtc
     1741 cggaacggct cggccagtac cggcagtgcc ttcgcattga ccgcatagag accgctcgtc
     1801 gcatcggcga agggtcgtcg cagcgcgagc ttcattccac gtcggagtag cccggtgccc
     1861 acccgcctcg ccgggctcgg caggtaacgg tagggctcgt aaccgtcacc cgagacgaag
     1921 cgtgaaccga ctgcgacgtc gcagccgccc tcgcgcacga ttgcgagcag ccgccccagc
     1981 tcggacggcg ggtgctgccc atcggcatcg acccggccgc agtacgcgta gccatggtcg
     2041 agggcccagc gatagccggc ggcgatcccg atcggcaaac cgcggttgct acccagcgag
     2101 aggacttcgg ctccgcgtcc gcgtgcgatc tcggctgtgc gatcggtcga gccgtcgtcc
     2161 accacgagca cgtccgcgct gggcagctcg cgcctgaggt cgtcgaggac ggccgggagg
     2221 ttctcctctt cgttccacgc cggaatgaag accacggtgt cactcatggc tgcgaccgtc
     2281 ctcgttccag ctcttcctcg gctgctcgca gctggcgcga ccgcactgtc agggcgccga
     2341 gctcccagat gaccgccgag ggcgtgggaa cgactcgcgg cgcttcgaag ctcgtccgga
     2401 tcccgtgtgc ggtgtacagg cgccggaaga accagcgggc gcggtagacg tgaggtggtg
     2461 tgcagatcac gaccgcgtgg tcgatctggc gctctagcag gagcggtagc gtccgcgcag
     2521 cgttctccgc cgtcgtcgac gcggtcggct ccagtacgag ctcaacctcc ggtccactcc
     2581 agagcttctg catctgctcc gcctcggagg caccgccgct cggcgcccaa ccgctgaaca
     2641 cgacgacccg aggctcgagc cgctcggcca gctgtgcggc ctgagcgact gctcgccggc
     2701 agccgcgact gatgcggtac gcgccggccc ggtcgcggcg gctgctcccg agcacgacga
     2761 aggccggtgg cgtcatgtcg gaagcgtacg cgcgcccgca tcgacttccg tcaattctcc
     2821 gcttagagtc ggcctcgcgg ccggaaaccg catcgaacga gaggaggaga gatgttgaag
     2881 gagtttcgag acttcttgct ccgggggaac atcgtggaac tggcggtggc gttcgtgatg
     2941 ggccttgcgt tcgcggccgt cgtcaactcg ctcgtgaacg atctgatcat gccggtgatt
     3001 gcaatgatca tcgggaagcc gaacttcagc gacctgacgt ttacgatcaa cgacgcgaag
     3061 ttccgctacg gcgcattcat cacgaacgtg atccagttcg tcgcgatcgc cggctcggtg
     3121 ttcttcttcg tggtgaagcc cgtgaacgca ctgctgacgc gctttcgctc gccggcagag
     3181 gaagggatgc cggacgagga gcgtcggcac caggagctgc tggccgcact gcggggttcc
     3241 gcagcgacga atcagtagac gcgaagagcc gccgagaccc cgaggtttcc accgccgcag
     3301 cgggcggtga tcaggtagcg ccctggacga atggtcgtcc ggatggcgac gagacgggaa
     3361 aacgtcccgc tcgaacgggc gcgcgttgcg acggagtgct gcacgaaggc gtggccggcg
     3421 aacggcggag agatcagata gacaacatcg cccgcccgac agcccttgcc gctgaccgtc
     3481 accgtcgcgc cgcgcggcac gctgaccgga tgcacgacga gccgcggcgt cgcggcgatg
     3541 atgggctcgg cgaccacgaa cgccaggacg gtcgcggctg cgatcaggcc ggcaacgtct
     3601 ctccatcgcc caggcatgac gtcatcatga cccaacggca cggggtcgat tcggatcgaa
     3661 gttcgccgcc gtgccagtta cgctcgttgg atgaccgttt gtccgagctg tggctcagag
     3721 aatcccgagg ggttccgttt ctgtgggtcc tgcggagcgg cgctcgccga ggcgccctcg
     3781 gcgcgcgagg tccgcaagac cgtgaccatc ctcttctgcg acgtctccgg ctcgaccgca
     3841 ctgggcgagc gcctcgactc ggagtcgctc cgccgagtga tggagcggta cttcacgctc
     3901 gcgcgcaacg tgctggaacg ccacggcggc acggtggaga agttcatcgg cgatgcggtc
     3961 atggccgtct tcggaatccc
//
LOCUS       DASVEU010000015         5925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_985044,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000015 DASVEU010000000
VERSION     DASVEU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985044"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VF225_00635"
     CDS             complement(<1..835)
                     /locus_tag="VF225_00635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7143788.1"
                     /translation="MRYVVTGAAGFIGSHLSETLIARGHDVAGVDCFTDYYDPALKEA
                     NAEGLDVARLDLAHEDLDLSGVDGVFHLAGQPGVRSFGDVFEEYVRRNLLASRRVFEA
                     AAAAGVRVVFASSSSIYGDAERYPTPEETAPRPISPYGITKLGCEQLAHAYTQGFGLD
                     AVVLRYFTFYGPRQRPDMALARVVDALTRGSSFELYGDGLQSRSFTYVADGIAATIAA
                     MESARAGAVYNVGGGEEATMREAIATLERISGRTLDLVERRAAAGDVRRTSADATRIE
                     RD"
     gene            complement(832..2142)
                     /locus_tag="VF225_00640"
     CDS             complement(832..2142)
                     /locus_tag="VF225_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143789.1"
                     /translation="MRAEAAVFAAARSSRWPFVIAVAVVVGGYAGLLAWDVAHYDWLR
                     GYDAYANSLYSDVIRLHHRLPTTSETDVWHTPPLFFALAALVESHRGVQFLDGAAALA
                     VVIFAGLIARELFPRSRLIQIAALAFAALTPVLTRTAVMYHPEPLATALAGAGLYVTV
                     RTLARGSHVLRGGAVAGLLFGLAILTRTWSVAPAAACFLGLILFAWLEREPRGLYSAG
                     LFVGIVLILTLPWFVHQARSHGSPFAFNRPAPKEQFFSRRPASFYLSLDVGTVLSHPY
                     APNFANHLLPVLYTDWWGDYWRYFEIPYENISAPPLLPPKYEKPRVRQNYVGVAPTLL
                     GVAGFVGIVWAALRRRSPALLVLPLAIVLLGLEFLVFQIAYPHADGDTIKATYLLNAV
                     APLAVCAAYALGWLRRAGRLLTIAVFGLLVYAAVLDISFLVLRT"
     gene            complement(2132..3112)
                     /locus_tag="VF225_00645"
     CDS             complement(2132..3112)
                     /locus_tag="VF225_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7143790.1"
                     /translation="MTAVLFTCAGQRVDIVGAFRRAGATAVAADANPLAPALYHANVH
                     ALVPRIEDPGYVPALRALVEQHDVRLIVPLTDLDQVVLARSRAELGAVVLLPDAEIVG
                     RLGDKYLAHLLFEERGIATPPTWLPNGVPDDAAYPLLVKARHGFGSRHIYRAADAAQL
                     GFFLGYTPVESIVQACLGGEEFSIDVFCDLDGRCLNAIPRTMIESKGGESIKGMSIRD
                     EQLIELARDVAEKLRLVGPANIQCFREADGSHYLTDINTRFGGGFPLPLAAGGRYPEL
                     ALALARGEHPEPRLGDFREGIVMTRFFSDLSLTPNGDGTLKPLSVDRSEG"
     gene            complement(3118..4320)
                     /locus_tag="VF225_00650"
     CDS             complement(3118..4320)
                     /locus_tag="VF225_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143791.1"
                     /translation="MRRSSLAAAGLIGALALVAGWNAYKYPSGAGYDVRQHREYADLL
                     IHHGRIPGPESRSEYYTPPVFYALAGVATVIGEHVHPGDPHKLGQVLNWFVLLATAGV
                     LWLLARELFPRRPWLQLSALAFFCFLPVVLRVGAMFHPEPLSMLLCSIGLLLGTRMLG
                     RHEYRWQLAVATGVALGLAQLVRAFSLWTFAAVVLGFAAARAWRPLVVIVLVTVVVTA
                     PWYVRQAIEYGNPVFDRPTKHEAIWNRRPARFYVGLGLPQVITDPIRPHFRNEAIPTT
                     YSEVWGDYFGVWRGSRRDQSIIGLLPTLLAVVGWAVLLARSRRLPARLPAALLPGLGI
                     LGYLYFTISYPTPDGDVLKASYMLTTAPAWALAFGYAIDRLPGRSRLVVAAILAASAL
                     ASLPFLLY"
     gene            complement(4310..5521)
                     /locus_tag="VF225_00655"
     CDS             complement(4310..5521)
                     /locus_tag="VF225_00655"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7143792.1"
                     /translation="MPGGRKPRLLVLNQYYWPGVEATAQLLTELCESLAEEMDVRVVT
                     GVLHGHEDEPRDLVQNGVRVIRVPSTSFERSKLAARASNYITYLANALLRSLRGVRPD
                     VVLCMTDPPIIADIALLVARRYRAPFVVISQDVFPEIAVQLKRLENPALMSLLRSLVS
                     LYLRRADRVVAIGDTMRKRLEEKGARPERVRVIPNWVDTKRLRPSEDGNEWSTGVGLD
                     DKFVVMHSGNVGHAQDLDSLVRSATFLRDLDDLRIVIIGMGARHAELVALSKQLEVDQ
                     VRFLYYQPRGVLPSSLSAADVHVVGLAPGLAGYVVPSRLYGILAVARPVIVAADPESE
                     TAQVVERAGCGIVVSPGRPELLARAIRDAHDGKYDLDAMGARGREWVVREADRSVALR
                     RYRDLLRELAS"
     gene            5553..>5925
                     /locus_tag="VF225_00660"
     CDS             5553..>5925
                     /locus_tag="VF225_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143793.1"
                     /translation="MSNRPLRILFAAPAWSPSRAFGGPVVAAGELVRRLVARGYDIDV
                     LTTTIVDLEQRPSPRSSAGVVDGANVRYLGTPLRYRWMGITPTLPAELARLPRPDVVH
                     VFGFRDPVTTGTAAWCRLVRVP"
BASE COUNT         1086 a         1958 c         2043 g          838 t
ORIGIN      
        1 ggtcgcgctc gatcctggtt gcgtccgcgg aggtgcgcct cacgtctccg gccgccgccc
       61 ggcgctcgac gaggtcgagg gtccggcccg atatgcgttc cagcgtcgcg atcgcctcgc
      121 gcatcgtcgc ttcctcccca ccgcccacgt tgtagacggc gcctgcgcgc gcgctctcca
      181 tcgccgcaat cgtcgcggcg atcccgtcgg cgacgtacgt gaacgaccgc gactgaagcc
      241 cgtcgccgta gagctcgaac gatgagccgc gcgtcagcgc gtccacgacg cgggcgagag
      301 ccatgtccgg gcgctggcgc ggaccgtaga acgtgaagta gcgcaggacg accgcgtcga
      361 gcccgaagcc ctgcgtatag gcgtgcgcga gttgctcgca acccagcttc gtgatcccgt
      421 acggggagat cgggcgcggc gcggtctcct cgggtgtcgg gtatcgctcc gcatccccgt
      481 aaatcgacga cgacgacgcg aagacgaccc ggacgcctgc ggcggcggcg gcctcgaaca
      541 ctctgcggct cgcgagcagg ttgcggcgca cgtactcctc gaagacatct ccgaagctgc
      601 gcacaccggg ctggccggcg aggtggaaga caccgtcgac cccggagagg tcgagatcct
      661 cgtgtgccag atcgaggcga gcgacatcga gcccttcggc gttcgcctcc ttcagcgccg
      721 gatcgtagta gtccgtgaag cagtcgacgc cggccacgtc gtggccgcgc gcgatgagcg
      781 tttccgacag gtgcgacccg atgaaccctg ccgcgccggt caccacgtat ctcatgtccg
      841 aagcaccaga aatgaaatat caaggacggc ggcgtaaacg agcaagccga agacggcgat
      901 ggtcaggagc ctcccggcgc gacgcagcca acccagcgcg tacgccgcgc agaccgcaag
      961 gggcgccacc gcgttcaaga ggtaggtcgc tttgatggtg tccccgtcgg catgtgggta
     1021 ggcgatctgg aagacgagga actcgaggcc gagcaggacg atcgcgaggg gtaagacgag
     1081 cagggccggg cttcggcgcc gcagcgccgc ccacacgatg ccgacgaagc ccgcgactcc
     1141 cagcagtgtc ggcgcgacgc cgacgtagtt ctgacgaacc cgtggtttct cgtacttcgg
     1201 cggcagcaaa ggaggagccg agatgttctc gtacgggatc tcgaagtaac gccagtagtc
     1261 cccccaccag tccgtgtaca gcacggggag gaggtgattc gcgaagttcg gggcataggg
     1321 gtgggagaga acggtgccga cgtcgaggct gaggtagaag ctggcgggcc gtcgtgagaa
     1381 gaactgttcc ttgggggccg gacggttgaa tgcgaacggg cttccgtgag accttgcttg
     1441 gtgaacgaac cacggcagcg tgagaatcag cacgatgccg acgaacaggc cggcgctgta
     1501 gaggccgcgg ggctcccgct cgagccaggc aaagaggatg agcccgagaa agcatgctgc
     1561 cgccggcgcg accgaccacg tgcgagtcaa gatcgcgagg ccaaagagaa ggccggcgac
     1621 ggcgccacct cgaagcacgt ggctcccgcg cgcaagcgtt cggacggtca cgtagaggcc
     1681 tgccccggcc agggcggtcg ccagcggctc ggggtggtac atcaccgccg tccgtgtcag
     1741 gacaggtgtc agggcggcga acgcgagcgc ggcgatctgg atcaggcgcg agcggggaaa
     1801 gagctcgcgt gcgatgagcc ccgcgaagat gacgaccgca agcgctgcgg caccatcgag
     1861 gaattgcacg ccccggtgcg attcgacgag tgcggccaac gcaaaaaaga gcggtggcgt
     1921 gtgccagacg tcggtctcgc tcgtcgtcgg caaccggtgg tgcaggcgta tgacgtcgct
     1981 gtagagggag ttcgcgtagg cgtcgtatcc ccgaagccag tcgtagtgag cgacatccca
     2041 ggcgagcagg ccggcgtagc cgccgacgac gacggctacc gcgatcacaa agggccaccg
     2101 agaggagcgc gccgcggcga agaccgcggc ttcagccctc actgcggtcg accgaaagcg
     2161 gcttcagtgt cccgtcgccg ttcggggtga ggctgagatc ggagaagaag cgtgtcatga
     2221 cgatgccctc gcggaagtcg cctaggcgcg gctcgggatg ctcgccccgg gcgagtgcga
     2281 gcgcgagctc cggatagcgt ccgccggcgg cgagcggcag cgggaacccg ccgccgaacc
     2341 tcgtgttgat gtcggtcagg tagtgcgacc cgtcggcttc gcggaagcac tggatgttgg
     2401 ccgggccgac gagccgcagt ttctcggcga cgtcgcgcgc cagctcgatc agctgctcgt
     2461 cgcgaatgct catccccttg atcgactcgc cccccttcga ctcgatcatc gtgcggggaa
     2521 tcgcgttcag gcagcggcca tccagatcgc agaagacgtc aatcgagaac tcctcgccgc
     2581 ccaggcacgc ctgcacgatc gactcgaccg gcgtgtagcc gaggaagaaa ccgagctgcg
     2641 cggcgtcggc cgcacggtag atgtgccgcg agccgaagcc gtggcgcgcc ttcacgagca
     2701 gcggatacgc cgcatcgtcg ggcacgccgt tcggcagcca ggtcggcggg gtcgcgatcc
     2761 cgcgctcctc gaacaggaga tgtgccaggt acttgtcacc gagacgcccg acgatctccg
     2821 cgtcgggaag caacaccacc gcgccgagct cggcacgaga gcgcgcaagc acgacctggt
     2881 cgagatcggt cagcggcacg atcagccgga cgtcgtgctg ctcgacaagc gcacgcagcg
     2941 ccggaacgta gccagggtcc tcgatccggg ggacgagtgc gtgcacgttg gcgtggtaga
     3001 gcgcgggggc gagcgggttg gcgtctgctg caacggccgt ggcgccggcg cgcctgaacg
     3061 cgcccacgat gtccacgcgc tgccccgcac acgtgaagag aacggccgtc acggcggtca
     3121 gtacagcagg aaaggcaggc ttgcgagcgc cgaggcggcc aggatcgcgg ccacgacgag
     3181 gcgcgagcgg ccgggcagcc ggtcgatcgc gtaaccgaag gcgagcgccc acgccggggc
     3241 agtcgtgagc atgtaactcg ccttcaagac atcgccgtcc ggagtgggat agctgatcgt
     3301 gaaatagaga taccccagga ttccgagacc cgggagcagc gcggccggca ggcgtgccgg
     3361 cagtcgccgt gaacgtgcca gcagaaccgc ccagcccacg acggcgagca gcgtcggcag
     3421 aagcccgatg atgctctggt cccggcggct ccccctccaa acgccgaagt agtcccccca
     3481 cacctcgctg tacgtcgtcg ggatcgcctc gttgcggaaa tgcggtcgga tcggatccgt
     3541 gatgacctgc ggcagcccga gtccgacata gaagcgggcg ggccggcggt tccagatcgc
     3601 ctcgtgcttc gtcggccggt cgaagacggg gttgccgtac tcgatcgcct gccggacgta
     3661 ccagggcgcc gtcaccacca cggtcacgag gacgatcacg acgagcggcc gccacgcgcg
     3721 ggctgccgcg aagccgagca cgaccgccgc gaaggtccag agcgagaagg cgcgcaccag
     3781 ttgcgccaac cccagcgcca cgcccgttgc gacggcgagc tgccagcggt actcgtgacg
     3841 tccgagcatg cgcgtcccca gcagcaaccc gatcgagcag aggagcatcg acaacggctc
     3901 gggatggaac atggcaccca cgcgcagaac gacaggcagg aagcagaaga aggcgagcgc
     3961 agagagctgc agccacggcc ggcgcgggaa gagctcgcgt gcaagcagcc agaggacacc
     4021 ggccgtcgcg agcaagacga accagttcag cacctggccg agcttgtgcg ggtcacctgg
     4081 atggacgtgc tcgccgatca cggtcgccac gccggcgagc gcgtagaaaa ccggtggtgt
     4141 gtagtactcg ctgcgcgatt ccggcccggg aatcctcccg tggtggatca acaggtcggc
     4201 gtactcgcga tgttggcgca cgtcgtagcc cgcgccgctc ggatacttgt acgcattcca
     4261 tcccgcgacg agggcgagcg cgccgatcaa gcccgcagca gcgagcgagc tacgacgcaa
     4321 gctcgcgcag aaggtcgcga tagcgtcgca gggcgacgga ccggtccgcc tcccgtacca
     4381 cccactcgcg cccgcgagcg cccatggcat ccaggtcgta cttgccgtcg tgtgcgtcgc
     4441 ggattgcgcg cgccagcaac tcgggacgac cgggtgagac gacgatgccg cagccggccc
     4501 gttccacgac ctgtgccgtc tcgctctcag gatcggcggc gacgatcacc ggcctggcga
     4561 cggcgaggat tccgtagagc cggctgggaa cgacgtagcc ggccaggccc ggcgccagcc
     4621 cgacgacgtg cacgtccgct gctgagagcg aagatgggag gactccgcgc ggctggtagt
     4681 agaggaaccg gacctgatcg acctcgagct gcttcgagag cgcaacgagc tcggcgtgac
     4741 gcgcccccat gccgatgatc acgatcctga ggtcgtccag gtctcgcagg aatgtcgcgg
     4801 aacgaacgag tgaatcgaga tcctgcgcgt ggccaacgtt accgctatgc atgacgacga
     4861 acttgtcgtc cagtccgact cccgttgacc actcgttgcc atcctcggaa ggccgcagcc
     4921 gcttcgtgtc cacccagttt ggaatgacac gtacccgttc aggccgtgca cccttctcct
     4981 cgagccgctt cctcatggtg tccccgatcg cgaccacccg atcggcacgg cgcagataga
     5041 gcgagacgag cgagcgcaac aaactcatca acgccggatt ctccaaccgc ttgagctgta
     5101 ccgcgatctc cggaaagaca tcctggctga tgacgacgaa aggcgcgcga taccggcgcg
     5161 caaccagaag cgcgatgtcg gcgatgatcg gcggatcggt catacagagg acgacgtccg
     5221 gccgcactcc ccggagactc cgcaggagcg cgttcgccag gtaggtgatg tagttcgagg
     5281 cacgagcggc aagcttcgat cgctcgaacg acgtcgacgg gacgcgaatc acccggacgc
     5341 cgttttgcac tagatcccgt ggctcatcct cgtgcccgtg gaggacgccc gtgacgacac
     5401 gtacgtccat ttcttccgca agcgactcgc agagctcggt gagcaactgt gcggtcgcct
     5461 cgacgcccgg ccagtagtac tggttgagaa cgagcagacg tggcttgcgg ccgccgggca
     5521 tcggcacaag gctagggcac tacgctggtg gcgtgagcaa ccgaccgctg cgcatcctgt
     5581 tcgccgctcc tgcctggtcg ccctcacgag cgtttggcgg tcccgtcgtc gcggccggcg
     5641 agctcgtcag acgactcgtc gcgcgcggat acgacatcga cgtcctcacg acgacgatcg
     5701 tcgacctgga gcagcgccct tcgccacgat cgtcggcggg tgtcgtcgac ggagcgaacg
     5761 tccgctacct ggggacgccg ctccgctatc gctggatggg aatcaccccg acactcccag
     5821 cggagctcgc gcggctacct cgtccggacg tcgtgcacgt cttcggcttt cgggatccgg
     5881 tgacgaccgg gacggcggcg tggtgccggc tcgtacgcgt tccct
//
LOCUS       DASVEU010000016        16048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_598130,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000016 DASVEU010000000
VERSION     DASVEU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598130"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VF225_00665"
     CDS             complement(<1..1392)
                     /locus_tag="VF225_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7143794.1"
                     /translation="MDVRARHLRLLTETIAAVNSTLDLEEVLELVASKVADALRTEAC
                     FVYLYDERTNELILRATHGTSVEEMTKRPRLRPGEGITGTAAADRAPVMIPSQAHLDP
                     RFKQFANLSEDDYESILAVPILARDKLAGALNVRTLAPREFTQDEIELLVAIADQVAQ
                     TIQHAQLYAQAQSRVAELEALAKISEAVSESLYLEESLEAIVKTTMEAVGATGAALVL
                     EDGNIAWPEGRAGIYNVRLPLRWKRRQIGELVCDRDTPFTDEERALLVSIAHHAAVAL
                     EHGRAVMRGVLAQEIHHRVKNNLQTVASLLRLQARATDTDPRKALEDSVHRILAIAAV
                     HEVLTEQREEVVELRELLERLRAMLVQGLAGGKEVQADLESVSIAGNRATALALVFSE
                     LLQNALEHGGNSVQIELAQRNGEVVLSIADNGSGIEAEATGTGLSIVQALVRDELQGR
                     LNLHSEEGTRAEVV"
     gene            1415..1888
                     /locus_tag="VF225_00670"
     CDS             1415..1888
                     /locus_tag="VF225_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX7143795.1"
                     /translation="MDTYDWLLFLHVTGAFLVLGGAVMAGIFNFAALQRERPSEIAVL
                     FRLARLAVIAIGAGMVLTLGFGLWLVHQVGYGWGETWIVLALVLWVLANALGGIGGGR
                     EKQTRELAERLAAEGDAPSQELSSRLRDPVWLGLSWGSGVVIVAILALMIWKPGA"
     gene            1888..2334
                     /locus_tag="VF225_00675"
     CDS             1888..2334
                     /locus_tag="VF225_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143796.1"
                     /translation="MLALVRPDSWNFPLFLHVLGAIFLAGSIGAVTVLAVAARKRTEN
                     AALLSKVAFRTLIFVVLPSWIVMRVGAQLIADKEFPDKTPGWVDVGFVVSEPGLIILI
                     ALGVLAWASARRGGVGRASVAVAILAPVYLAALAVAWWAMSAKPGG"
     gene            2400..2654
                     /locus_tag="VF225_00680"
     CDS             2400..2654
                     /locus_tag="VF225_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143797.1"
                     /translation="MSALPLNLVDTAGFLVADNRGRVVGKVECPMYGTLPDVPDALSV
                     RAGFFSRHRRLVPADTIADVDPTSQVVGLRVARETIRRFL"
     gene            complement(2887..4251)
                     /locus_tag="VF225_00685"
     CDS             complement(2887..4251)
                     /locus_tag="VF225_00685"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7143798.1"
                     /translation="MAQVETRNRATSSEDSRKRVHGRIEKEGIEFVLLWFTDIEGHLK
                     SFAVTPSEIEAALDDGMGFDGSSITGFNAIEESDMVAIPDPETFQLMPWKEGETRVAR
                     MICDIVTPDGNPYEGDPRYVLRRALDRMAALGFDTFNVGPELEYFLFRDDKGTETLDE
                     GGYFAMTTLDAASELRQETVRALEGMGIPIEYVHHEVGPSQHEIDMRYSDALAMADHT
                     VTYRLIVKEIAKKSGYHATFMPKPIFGENGSGMHTHQSLFKDGRNAFFDGDDEWNLSA
                     AGKAFIAGQLKHAREISAVFAQWVNSYKRLVPGYEAPVYVAWSRRNRSALIRIPLYKP
                     GAEQATRAEIRCPDPACNPYLTFAALLHAGLEGIEQGYELPDPMETNLYHLTAEQRKE
                     RGIVSLPETLGEAVDALAGSELVRKALGPHIFDRYVELKRAEWDEYRVQLTEWELKKY
                     LSVL"
     gene            4347..5330
                     /gene="rfbB"
                     /locus_tag="VF225_00690"
     CDS             4347..5330
                     /gene="rfbB"
                     /locus_tag="VF225_00690"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7143799.1"
                     /translation="MRLLVTGGAGFIGSHFVRRMLAAGEDVVVLDKFTYAGNPANLPE
                     GIDCRRGDIATRGDVDQIGPVDAIVNFAAETHVDRSILGPEDFVRTDVLGTLVLLQAA
                     EELGARFVQVSTDEVYGDVEPGWSSREDDPLRPSSPYSASKAGGDLQVLAAVRTFGAN
                     ASITRGSNTYGPNQYPEKVLPLFATNALDGEPLPLYGDGRQVRDWLHVDDHCAAIELV
                     LRQGEAGEVYNVGANEEHENVELTKLIVEHLGVDPLLVRNVEDRAGHDRRYSLETAKV
                     HSLGWQPRRDWADGVRATIDWYRDNRDWWEPIKRSGGFREYYDKQYATRLS"
     gene            complement(5337..6755)
                     /locus_tag="VF225_00695"
     CDS             complement(5337..6755)
                     /locus_tag="VF225_00695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7143800.1"
                     /translation="MKIAILPPRYRSPEQIGRGGMGDIYRATDSVLGRVVAIKVLADR
                     YAHDESVRERFTREALSAARLSGEPNTVTIYDVGEHDDRPYIVMEYLGGGSLDDVLRS
                     DGAQPPERVFTWLEEAARALDAAHREGVVHRDVKPANLMLDGEGHVHVADFGIASSAG
                     MESLTMTGTVLGTAGYFAPEQAQGDRASAASDRYALAIVAWELLTGGRPFESESTTAE
                     AAAHINAPIPAISAAGDFPRELDRVFGHALAKDPAERYATAGEFVAALRSAFSEAAGA
                     TGELAAVAPLPTAATRRMRGAEEVAYAPYPPDARSSRGAWPLLAALLLAGAVAGALLA
                     YFLTRGDGARPAPTTVVTHVRTVTTQGQVTTVSKPVTVTTAPSAGVAPSAGGAALNTA
                     GFRKMQAGDYAGALPLLERAVQKLQGSRSLDEAYADYNLAFTRFQLGSCEGVLELLDR
                     SEAIQGQRSEIDRLREQAQQSC"
     gene            6885..8495
                     /locus_tag="VF225_00700"
     CDS             6885..8495
                     /locus_tag="VF225_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX7143801.1"
                     /translation="MRRTLVLVSVLVLAAGSAAPTRTDAQPVPAGAATFLVTGHGWGH
                     GVGLSQYGAYGYAQKGVLYENIVLHYFPGTELGAAPVSRVRVLLTTGIAKLDIGSTAD
                     FKVRDGSGVVHIVAAGKYTLTPALKLAVDGQTAPRALPGPLLFQPGAAALGLKHRYRG
                     SIQIDVTAGKLRAINMVGLEQYLYGVVPSEMPFTWLPEALKAQAVVARSYALATRRTG
                     AFDLYSDTRSQVYLGIEHEKPSTNAAVNATAGQVVLYEGQVAKTYFFSTSGGRTMSAE
                     DAWGEPVPYLVSVPDPYDSISPHHDWGPLVFTGAKLAKVLKMKGRVVDLQPELNSSGR
                     IKVLNVIGTKGTLAMQGADLRRRLKLQSTWFSVGVLSLTAPTQPVTYGGRGKLTGIAR
                     GLTQAVLQQLQGDSWTDVASLKGDQDGVITALVKPTVTTRYRLSSGKVSAEPVRVPVA
                     PLARFYPARTQDRLTGYVRPASLAGTTVLIQRQQGEAWPTVGQATVEANGDFLAKLQL
                     TTGVYRARVSSGHGFVVGFSPVLQVSTS"
     gene            8510..9946
                     /locus_tag="VF225_00705"
     CDS             8510..9946
                     /locus_tag="VF225_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7143802.1"
                     /translation="MVLALALAPTAAAMPFRAGFVPTDPLAPKQYYLAQDHAFDAFPI
                     ELPVVNPVRVAIIDSGIDGSHPEFPRARIWQARSWVGGSPLTDEEGHGTFVAGMIAAS
                     IDNNEGIAGMAFPAQLVIAKIARADQSIDVRDEAEAIRWAVDVGARVINLSIGGLRDP
                     FDPRRDTFSRVEASAINYAVSRGAVLIAAVGNSDDAPQSPWPFASYPAALPHVLGVSA
                     LSPTGNVPQFSNRDRIYNDISAPGQEIYSTLPRALTATRPLCQNQGYSDCGPDEFRHA
                     AGTSFAAPQVTAAAAVMLALHPTLQPDQVIDVLERTATDMNASNGCKQCPLQRDASSG
                     WGRLDVAKAIGALEGVLPPPDRLEPNDDAGGSAARLGTKVTSVKATIDFWDDQIDVYR
                     LYLKKGHKVKLTLNGPQGSTSNLLLWKPGTKRVNDLHAQHLRAAQAIGPGLTHGIGYR
                     APASGWYYAEVKLTAGSFGPYELTISRR"
     gene            complement(9952..10596)
                     /locus_tag="VF225_00710"
     CDS             complement(9952..10596)
                     /locus_tag="VF225_00710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7143803.1"
                     /translation="MSAEVAETAIVYPGTVLGAGCRILDYAVVGKPPTLGPRSTTKQE
                     DLPPLELGPGTIVSTGAIVFAGTRIGARVIVGDQACVRERCDVGDEVVIGRGALVEND
                     TTIGELTKIQADAYVTAYSTLEDNVFIAPRVITTNDNFMGRTEDRHALRKGPTIRRGA
                     RVGAGAVLCPAVEIGEEAFVGAGAVVVRDVPPRVLVVGNPARVLRDVPSDELLG"
     gene            complement(10601..11587)
                     /locus_tag="VF225_00715"
     CDS             complement(10601..11587)
                     /locus_tag="VF225_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7143804.1"
                     /translation="MARTTRIAQVGLGQWGKNLARNFDELGDLAWLCDIDDERRAELA
                     QRYPTATMTGDFEDVLADDSVDAVVVATPVPTHHELARRSLEAGKHVFVEKPPAMRGA
                     EMEELCDLAEERGRVLMPGHLLLYHPGVQKLKEIVDSGDLGDVLCVYGNRQNLGTIRT
                     HENALWSLGVHDLSVLLYLIGEEPTELWAHGHAFLNPNVEDIVFCYLRFPSGKIAHLH
                     LSWLDPHKIRRITVVGRDKMAVFDDMEPERKVTVYDKAPEEPTTIYGEWRTRTGDIHS
                     PKVPLLEPLRLECRHFLNLVAGQGDPMRAARDGVLVVRALEQLQASLETVTA"
     gene            complement(11590..12846)
                     /locus_tag="VF225_00720"
     CDS             complement(11590..12846)
                     /locus_tag="VF225_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7143805.1"
                     /translation="MKSDVAIVGAGYVGMPLARVFAEAGKNVVLVDVNEDVVDGINRG
                     ESHIGDVPSEALKTLVDAGRVLATSDYDALQEVDAILIALPTPLSSHREPDLSIVESA
                     AAEIAPRLRKGQIVVLESTTYPGTTRESLQPILEGSGMKAGEDFHLAFSPERVDPGRT
                     DWTTKNTPKLVGGLTPTCTERAVELYRSAVDTVVPLSSPETAEMTKLLENIFRAVNIA
                     LVNELAQLCERMEVDVWEVVEAAETKPFGFMSFKPGPGLGGHCIPIDPFYLTWKAREY
                     DFYTEFIDLAGKVNANMPFFCRSLISQALNHGAEKSLKGSRILILGVSYKADIDDVRE
                     SPAKKIVELLHKAGTDVSYHDPHVPEFDGLRSVDYAPESYDCVVIVTAHSSIDYEDLV
                     ERAQVVVDLRNATGRNGTGNGKVWKL"
     gene            12898..12970
                     /locus_tag="VF225_00725"
     tRNA            12898..12970
                     /locus_tag="VF225_00725"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12931..12933,aa:Lys,seq:ctt)
     gene            13278..>16048
                     /locus_tag="VF225_00730"
     CDS             13278..>16048
                     /locus_tag="VF225_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7143806.1"
                     /translation="MLPEILALAGRTLAADAYSVWQYDDATGIWNVGARVGLSDEYVA
                     AAMAAIRGNTATVSLDAPIVAEDIERTAWLTPEHRSAHAAERTRSMMALPLHYGERVL
                     GTLVVYYREPRSFSEAEKSTAMLLANLAAAAIGTAELYQAQRRLAEDQRFVAEASELL
                     ASSLDYETTLSNLATLAVPRFADWCTIDMVGDDGSIHRLTVAHVDPEKVRWAQELADR
                     YPADTSAPYGVPNVIRTQEPELLREIPDDLLREATADTPELYAILEALGLKSSMCVPL
                     VARGRALGAITFVSAESGRRYEESDLATARDLARRAAISVDNARLFREAEGSRREAHE
                     SLAVVDAVFAAAPVGLAFMDTSFRYVRVNEALAAINGLPSDEHYGRTLREVLGEDLAS
                     EIEPLHRQVLESGVPILDRYVEQALPAAPGETRNWLVGYYPVLDADENKIGVGVVITD
                     VSEREQARAAAERAGARLAVLAEASQTLASTLDYESTLASLASLLVPRFADWYAVDIV
                     DENGLFRRLAVVHWDPAKSEWADKSRDLYPPDLEEDEGTGRAVRTGEAVLYRTISDEL
                     LASSTKDAAHNEILHQLGMASAMVVPLTAAGRTFGGLMLVSSDPARLYDDDDLEFAKH
                     LGRRAAVAVDNARLYRAAEERARAAIVVEHVADGVLLVDQDEIIRLWNPAAEHITGLA
                     APKTVGRSTAEVFSPWKTIAELAERGEPRPETQPVEVNGRELWLSITGVGFEGGTVFA
                     FRDLTAERAVEKLKSDFVSTVSHELRTPLAAIYGAALTLRREDVPLGEPQRMGLLEVI
                     ASESDRLARIVNDILWASRLESGTMQTTIQKCDGVAIARSVVDAARHYIPPSIELAIK
                     APKKVPLVAADPDKARQVLTNLVDNARLYRAAEERARAAIVVEHVADGVLLVDQDEII
                     RLWNPAAEH"
BASE COUNT         2603 a         5450 c         5269 g         2726 t
ORIGIN      
        1 aaccacctcg gccctcgtcc cttcctcgct gtgaaggttg aggcggccct ggagctcgtc
       61 tcgcaccagc gcctggacga tcgataggcc cgttcccgtt gcttcggctt cgattcccga
      121 gccgttgtcg gcgatcgaga gcaccacttc gccgtttcgc tgcgctagct cgatctggac
      181 gctgttgccg ccgtgttcca gggcgttttg cagcagctcg ctgaagacca gcgccaacgc
      241 cgtcgctctg ttgccggcga tcgacacgga ttcgaggtcg gcttgaacct ccttgccgcc
      301 ggcgaggccc tgcacgagca ttgctcgcag acgttcgagc agctcgcgta gttccacgac
      361 ctcctcgcgc tgttccgtca ggacctcgtg cacggccgca atcgcgagaa tgcggtggac
      421 cgaatcctcg agcgccttgc gcgggtccgt gtctgtggcg cgggcctgca ggcgcagcag
      481 cgacgccacc gtctgcaggt tgttcttgac ccggtggtga atctcttgcg cgaggacgcc
      541 gcgcatgacg gcgcgcccat gctcgagtgc caccgctgcg tggtgcgcga tcgacacgag
      601 caacgctcgt tcctcgtccg tgaacggcgt atcgcgatcg cagacgagct cgccgatctg
      661 ccgccgcttc cagcgcaaag gaaggcggac gttatatatc ccagcgcgcc cttcaggcca
      721 ggcgatgttg ccgtcctcga ggaccagcgc cgcgccggtc gcgccgaccg cctccatcgt
      781 cgtcttcacg atcgcctcga gcgactcctc gaggtagagc gactcggaca ccgcctccga
      841 gatcttggcc agggcctcga gctcggcgac ccggctctgc gcctgtgcgt agagctgcgc
      901 gtgctgaatc gtctgcgcca cctgatccgc gatcgcaacg agcaactcga tctcgtcctg
      961 agtgaactcg cgcggcgcga gcgtgcgcac gttcagcgcg ccggcgagct tgtcgcgggc
     1021 aagaatgggc accgcgagga tcgattcgta atcgtcttcc gacaggttcg cgaactgctt
     1081 gaagcgagga tcgagatgcg cctgcgacgg gatcatcacc ggcgcccgat cggccgcggc
     1141 ggttccagtg atcccctcgc ccggccgcag gcgagggcgc ttcgtcatct cctcgacgct
     1201 cgtcccgtgc gtagcgcgga gaatgagctc attcgtgcgc tcgtcataga ggtagacgaa
     1261 gcaggcctcg gtccggaggg cgtcggcgac cttgctcgcg acgagctcga ggacctcttc
     1321 gaggtcgagc gtcgagttca ccgcggcgat cgtctcggtg agaaggcgca gatggcgagc
     1381 ccgtacgtcc actggggtac ctaggctagt cggcgtggac acgtatgact ggctgctgtt
     1441 cctccacgtc acaggggcat tcctcgtcct cggcggcgcg gtgatggccg ggatcttcaa
     1501 cttcgcggcg ctgcagcgag agcgtcccag cgagatcgcc gtgctgttcc ggctggcgcg
     1561 gttggccgtg atcgcgatcg gggccggaat ggtgctgacg ctcggcttcg ggctctggct
     1621 cgtacaccag gtcggctacg gctggggtga gacctggatc gttctggcac tcgtcctctg
     1681 ggtcctggcc aacgcactgg gcggtatcgg agggggacgc gagaagcaga cgcgagagct
     1741 cgccgaacgt ctcgccgcgg agggcgacgc cccgagccag gagctgtcgt cccggctgcg
     1801 cgatcccgtc tggctcggcc tgagctgggg gagcggagtc gtcatcgttg cgatcctggc
     1861 cctgatgatc tggaagccgg gcgcctgatg ctcgcactcg tccgccccga cagctggaac
     1921 ttcccactct tcctgcacgt cctgggcgcc atctttctcg ccgggtcgat cggggcggtg
     1981 accgtgctgg ccgtggcggc gcgaaagcgc accgaaaacg cggcgctgct gtctaaggtt
     2041 gcctttcgaa cgctgatctt cgtggtgctg ccgtcctgga tcgtgatgcg cgtcggcgcc
     2101 cagttgatcg cggacaagga gtttccggac aagacccccg gctgggtcga cgtcggtttc
     2161 gtcgtctcgg agccgggcct gatcatcctg atcgccctcg gcgtgctcgc ctgggccagc
     2221 gcgcgccgcg gcggagtcgg acgcgcctcg gtggctgtgg cgatcctggc gccggtctat
     2281 ctcgcggccc tggcggtggc ctggtgggcc atgtcggcga aacctggcgg ctgacccatt
     2341 tacagaaccc ttactgcgcg caggggaatt tccttgcaga cgaggaatag gttcgaacca
     2401 tgtccgcact ccctctgaat ctcgtggaca cggccggttt cctggtggcg gacaaccgcg
     2461 gtcgagtggt cggcaaggtc gagtgtccga tgtacggaac gctccccgac gtccccgacg
     2521 ccctctcggt tcgcgccggc ttcttctcgc gccaccgccg cctggtcccg gccgacacga
     2581 tcgccgacgt cgatcccacc agccaggtcg tcggcctccg cgtcgcccgc gagacgatcc
     2641 gtcgcttctt gtagacgagc gaaacgaagt cctacccttt acccgccgaa aatgctccgc
     2701 attttcggcg gtagaacgac cacgagctgc gctatgaagc agcagctcgc agtcggccgt
     2761 cagttggatc tcgtaggccg cagaagctca cgagaccggc cccaatcatc ctcctttttc
     2821 aggcggcgga caagtcaggt gctggcccgt cgacggcttc ccaagggaac ctgcgttccc
     2881 ccacgcctag agcactgaca agtacttctt gagctcccac tcggtgagct ggacccggta
     2941 ctcgtcccat tcggcccgct tcagctccac gtagcggtcg aagatgtgcg ggccgagcgc
     3001 cttgcgaacg agctccgacc ctgcgagtgc gtcgacggcc tcgccgagcg tctccgggag
     3061 cgagacgatt ccgcgctcct ttcgctgttc cgcggtcagg tgatagaggt tcgtctccat
     3121 cgggtcgggt agctcgtacc cctgttcgat cccctcgagg ccggcatgga gcagggcggc
     3181 gaacgtcagg tacgggttgc acgccggatc ggggcaacgg atctccgccc gcgtcgcctg
     3241 ctcggcgccg ggcttgtaga gagggattcg gatcagagcc gatcggttgc gccgcgacca
     3301 cgcgacgtac accggcgcct cgtagcccgg gacaagccgc ttgtatgagt tcacccactg
     3361 cgcgaacacg gcgctgatct ctctcgcgtg cttcaactgg cccgcgatga acgccttccc
     3421 ggcagctgag aggttccact cgtcgtcgcc gtcgaagaac gcgttgcgcc cgtctttgaa
     3481 cagcgactgg tgcgtgtgca tccccgagcc gttctcgccg aagatcggct tcggcatgaa
     3541 ggtcgcgtga tagccggact tcttggcgat ctccttcacg atcagccggt aggtgaccgt
     3601 atggtcggcc atcgcgagcg cgtccgagta ccgcatgtcg atctcgtgct gggagggccc
     3661 gacctcgtgg tggacgtact cgatcgggat gcccatgccc tcgagcgcac gaacggtctc
     3721 ctgtcgcagc tcgctcgccg catccagggt cgtcatggcg aagtagccgc cctcgtcgag
     3781 cgtctcggtg cccttgtcgt cgcggaacag gaagtactcg agctccggac cgacgttgaa
     3841 ggtgtcgaag ccgagcgccg ccatgcggtc gagcgcgcga cgcagcacgt agcgtggatc
     3901 cccttcatag ggattcccat caggcgtgac gatgtcgcag atcatccgcg cgacccttgt
     3961 ttcgccttcc ttccacggca tcagctggaa ggtctccggg tccgggatcg cgaccatgtc
     4021 cgattcttcg atcgcgttga acccggtgat cgacgagccg tcgaagccca tgccgtcgtc
     4081 gagggccgcc tcgatctcgc tcggcgtcac cgcgaagctc ttcaggtggc cctcgatgtc
     4141 cgtgaaccag agcaggacga attcgatccc ctccttctcg atccgaccgt gcacccgttt
     4201 gcgcgaatcc tcgctcgacg tcgccctgtt gcgtgtctcg acctgcgcca tgaaatcctc
     4261 ccttgaaacg cgaaaagcct caggcgcaga cgaggcgccc gaggctccat tgcctcactg
     4321 acggacggag tctatacggt ggcgccgtgc gcctactcgt caccggtggt gcggggttca
     4381 tcggctcgca cttcgtcagg cggatgctcg cggcgggcga ggacgtggtg gtgctggaca
     4441 agttcacgta cgcggggaat ccggccaacc tgcccgaagg gatcgattgc cggcgaggcg
     4501 acatcgcgac gcgcggggac gtcgaccaga tcggcccggt cgacgcgatc gtcaactttg
     4561 ccgccgagac ccacgtcgat cggtcgattc tcggcccgga ggacttcgtg cgcaccgacg
     4621 tcctggggac cctcgtgctg cttcaggcgg ccgaggagct cggcgcccga ttcgtccagg
     4681 tgtcgacaga cgaggtctac ggggacgtcg agccgggctg gtcgtcgcgt gaggacgatc
     4741 cgctgcggcc ctcgagccca tacagcgcct cgaaggccgg cggcgacctc caggtgctcg
     4801 cagcggtgag gactttcggc gccaacgctt cgatcacgcg aggttcgaac acctacgggc
     4861 ccaaccagta tccggaaaag gtgctcccgc tcttcgcgac gaacgccctc gacggcgagc
     4921 cgctgccgct ttacggcgac gggcgccagg tccgcgactg gctccacgtc gacgaccatt
     4981 gcgcggcgat cgagctcgtc ctccgacagg gcgaggcggg cgaggtctac aacgtcggcg
     5041 cgaacgagga gcacgagaac gtggagctca cgaagctgat cgtcgagcac ctcggcgtcg
     5101 atccgttact cgtccgaaac gtcgaagacc gcgctggaca cgaccggcgc tactcgctcg
     5161 agacggcgaa ggtccactcg ctcggctggc agccgcggcg cgactgggcc gacggcgtgc
     5221 gcgcgaccat cgactggtac cgcgacaacc gcgactggtg ggagccgatc aagcgctcgg
     5281 gcgggttccg cgagtactac gacaagcaat acgccactcg cctgagctaa cgctcactag
     5341 cagctttgct gcgcctgctc tcggagccgg tcgatctcag agcgttgacc ctgaatcgcc
     5401 tcggagcggt cgaggagctc gagcacgccc tcgcagctgc cgagctggaa ccgggtgaac
     5461 gcgaggttgt agtcggcgta ggcctcgtcg agtgacctcg agccctgaag cttctgcacg
     5521 gcgcgctcga gcaacggcag cgctcccgca tagtcacccg cctgcatctt cctgaacccg
     5581 gcggtgttga gcgctgcgcc accggcgctc ggggcaaccc cagcgctggg ggccgtcgtg
     5641 acggtgacgg gcttcgaaac ggtcgtgacc tgaccctgcg tcgtcaccgt tcggacgtgc
     5701 gtgactaccg ttgtgggcgc cggccttgca ccgtcgccgc gcgtgaggaa gtacgcgagc
     5761 agcgcacccg cgaccgcgcc ggcgagcagg agcgcggcga gtagcggcca ggcgccgcgc
     5821 gaggaacggg cgtcgggcgg gtaaggcgcg tacgcgactt cttcggctcc tcgcatgcga
     5881 cgcgtcgccg ccgtcggcag cggggcaacc gcggcgagct cccctgtcgc tccagcggct
     5941 tcactgaacg ccgacctcag tgcggcgacg aactcgcccg cggtcgcgta ccgctcggcc
     6001 ggatccttcg cgagcgcgtg cccgaagacg cgatcgagct cacgcgggaa atctccggca
     6061 gcagagatcg ccggaatcgg ggcattgatg tgcgccgccg cttccgcggt cgtactctcc
     6121 gattcgaacg ggcgtcctcc cgtgagcagc tcccacgcga cgatcgcgag ggcataacgg
     6181 tcgctcgccg cgctagcgcg atcgccctgc gcctgctcgg gcgcgaaata tccggcggtt
     6241 cccagcacgg tcccggtcat cgtcagggac tccatgccgg cagagcttgc gatcccgaag
     6301 tcggccacgt gaacatggcc ctcgccgtcg agcatcaggt tcgcaggctt gacgtcacga
     6361 tggacgacgc cttcacggtg cgccgcgtcg agtgcgcgcg ccgcttcttc gagccatgtg
     6421 aagacgcgtt ccggtggctg tgccccgtcg cttcggagca catcgtcgag cgacccgccg
     6481 ccgaggtact ccatgacgat gtacggacgg tcgtcgtgct cgccgacgtc gtagatcgtg
     6541 acggtattcg gctctcccga gagacgggct gcggagagcg cctcgcgcgt gaaccgttcg
     6601 cggacggact cgtcgtgggc gtagcgatcg gccagaacct tgatcgccac gacacggccg
     6661 agcaccgaat cggtcgcccg gtagatgtcg cccatgccgc cgcgtccgat ctgctcgggg
     6721 ctgcggtagc ggggcgggag gatggcgatc ttcacggcca aagtaaggaa acggatttcc
     6781 cccgttcatg gtcgcgcaaa cgatgagatg agggttccgt aaagaaagcc gctgctcatg
     6841 agaccctttg ctagcctcgg tcgcgtcctg gctgggtcag ctcgatgcgt cgaaccctcg
     6901 tactcgtctc cgttctcgtg ctcgccgccg gctcggcggc gcctacccga acggacgcac
     6961 agcctgttcc cgccggagct gcgacgttcc tcgtcaccgg ccacggttgg ggacacggcg
     7021 tcggcctgag tcaatacggg gcgtacggct acgcccagaa gggcgtcctc tacgagaaca
     7081 tcgtgctgca ttacttcccc gggacggagc tcggagccgc accggtctcg cgcgttcgag
     7141 tgcttctgac gaccgggatc gccaaactgg acatcggttc gacggctgac ttcaaggtgc
     7201 gcgacgggag cggggtcgtg cacatcgtcg ctgcagggaa gtacacgctg acgcccgcgc
     7261 tcaagctcgc ggtggacggt cagacagcgc cacgtgcgct gcccggcccg ctgctgttcc
     7321 agccgggggc cgccgccctc ggcctcaagc accgctatcg ggggtcgatc cagatcgacg
     7381 tcaccgccgg aaagctgcgg gcgatcaaca tggtcgggct ggagcagtac ctctacgggg
     7441 ttgtcccgtc ggagatgccg ttcacgtggc ttcccgaggc gctcaaagca caagccgtgg
     7501 tcgcgcgctc gtacgcattg gcaacgcgca ggaccggtgc gttcgatctt tattcggata
     7561 cgcggagcca ggtgtatctc gggatcgagc acgagaagcc gtcgacgaat gccgccgtca
     7621 atgcgacagc cggccaggtc gtgctctacg agggacaggt cgcaaagacg tacttcttct
     7681 cgacgtccgg cgggcgcaca atgtcggccg aagacgcgtg gggagagccc gtcccctacc
     7741 tcgtctcggt tccggacccc tacgactcga tctctccaca ccacgattgg ggaccgctcg
     7801 tcttcacggg cgcgaagctc gcaaaggtcc tgaagatgaa gggtcgcgtc gtcgatctgc
     7861 aaccggagct gaactcctca ggccggatca aggtgctcaa cgtcatcggc acgaagggta
     7921 cgctcgccat gcagggcgcg gacctgcgcc gcaggctaaa actgcaatcg acctggttca
     7981 gcgtcggcgt cctctcgctg accgctccca cgcagcccgt cacgtacgga ggacgcggca
     8041 agctgacggg catcgctcgc ggactgacgc aggcggtgct gcaacaactg cagggagaca
     8101 gctggaccga tgttgcgtcg ctgaaaggcg atcaggacgg ggtaatcacg gcgctcgtga
     8161 agccgacggt tacgacgcgc taccgcctgt ctagcggaaa ggtgagcgcg gagccggtgc
     8221 gcgttccggt tgcgccgctc gcgcgcttct atccggctcg aacgcaggac cggctcaccg
     8281 gctacgtccg cccggcgtcg ctcgccggga cgacggtcct gatccagagg caacagggcg
     8341 aggcctggcc gacggtcggg caggccaccg tcgaggcaaa cggtgatttt cttgcgaagt
     8401 tgcagctcac gaccggggtc taccgcgccc gcgtgagctc gggccacggg ttcgtggtcg
     8461 gcttctcgcc ggtgctccag gtctcgacct cgtgaggaca acggctctcg tggttctggc
     8521 gctcgcgctg gcgccgaccg ccgctgcgat gccgtttcgt gcgggcttcg tcccaaccga
     8581 ccccctcgcg ccgaagcagt actacctggc tcaggaccac gcgttcgacg cgttcccgat
     8641 cgagcttccc gtcgtcaacc cggttcgggt ggcgatcatc gactcgggga tcgacggcag
     8701 tcacccggaa ttcccgcgcg cacggatctg gcaggcgagg agctgggtcg gaggcagtcc
     8761 actcaccgac gaggaaggcc acgggacttt cgtcgcagga atgatcgcgg cctcgatcga
     8821 caacaacgaa ggcatcgccg gcatggcgtt tcccgcacag ctcgtgatcg ccaagatcgc
     8881 acgtgcggac cagtcgatcg acgtgcgcga cgaggcggaa gcgatccgtt gggcagtcga
     8941 tgtcggtgca cgcgtgatca acctctcgat cggcggcctg cgtgatccgt tcgatccacg
     9001 ccgcgacacc ttctcgcgcg tcgaggcgag tgcgatcaac tacgcggtga gccgcggtgc
     9061 cgtgctcatc gctgccgtcg ggaactccga tgacgcgcca cagtccccct ggccgttcgc
     9121 cagctatcca gctgcgctac ctcatgtgct cggagtgagc gcgctgagcc cgaccggcaa
     9181 cgtgccgcag ttctcgaacc gagaccgcat ctacaacgac atctcggcgc ccgggcagga
     9241 gatctactcc accctgccgc gcgccctgac cgcgacgcgc ccgctctgcc agaaccaggg
     9301 gtattccgac tgcggcccgg acgagttccg gcacgcggcc ggcacttcat tcgccgcgcc
     9361 acaggtgacg gcggcggcgg ctgtcatgct ggcgctccac ccgacgctgc agcccgatca
     9421 ggtcattgac gtgctcgagc gcaccgcgac ggacatgaat gcctcgaacg gctgcaaaca
     9481 gtgcccgctg cagcgcgacg cgtccagcgg ttgggggcga ctcgacgtcg cgaaggcgat
     9541 cggcgcgctc gaaggcgtgc tgccaccgcc ggaccggctc gagcccaacg acgatgccgg
     9601 cggctctgcg gccaggctcg gcaccaaggt cacctccgtc aaagcgacga tcgatttctg
     9661 ggacgaccag atcgacgtgt accgcctcta tctgaagaag ggccacaagg tgaagctcac
     9721 cctcaacggg ccgcagggct ccacctcgaa cctgctgctg tggaagcccg ggacgaagcg
     9781 cgtgaacgac ctccatgccc aacatctccg cgccgcccag gcgatcggcc ccggtcttac
     9841 gcacgggatc gggtacaggg cgccggcgag cggctggtac tacgcagagg tgaagctcac
     9901 ggcggggagc tttgggccgt acgagctgac gatctcgcgg cgctagagga cttagccgag
     9961 cagttcgtcg gacggcacgt cgcgcaggac acgcgcggga ttgcctacca ctagaacgcg
    10021 cggcgggacg tccctgacga ccacggcacc cgcgccgacg aacgcctctt cgccgatctc
    10081 gaccgccgga cagaggacgg ctccggcgcc gacgcgcgcc cctcgccgga tcgtcgggcc
    10141 ctttcggagg gcgtgcctgt cctcggttcg ccccatgaag ttgtcgttgg tagtgatcac
    10201 gcgcggcgcg atgaacacgt tgtcctcgag ggtcgagtac gccgtgacgt acgcgtccgc
    10261 ctggatcttc gtcagctccc cgatcgtcgt gtcgttctcg acgagcgccc cccgcccgat
    10321 caccacctcg tcaccgacgt cacagcgttc gcggacgcac gcctggtcgc cgacgatgac
    10381 ccgcgcgccg atgcgcgtcc cggcgaagac gatcgcgccg gtggagacaa tcgtgccggg
    10441 ccccagctcg agcggtggca ggtcttcctg cttcgtcgtg gagcgcgggc cgagcgtcgg
    10501 cggcttgccg acgaccgcgt aatcgaggat tctgcaacca gcgccgagca ccgtgcccgg
    10561 atagacgatc gcggtctcgg cgacctcggc gctcatagtt tcaagccgtg actgtttcga
    10621 gcgacgcctg cagctgctcg agggcccgca ctacgaggac accgtcgcgc gcagcgcgca
    10681 tcggatcgcc ctggccggcc acaaggttca ggaagtgccg gcactcgagg cgcagcggct
    10741 cgagcagcgg caccttcggg ctgtggatgt cgcctgtgcg cgtccgccac tcgccgtaga
    10801 tcgtcgtcgg ctcttcaggt gccttgtcgt acaccgtgac cttgcgctcg ggctccatgt
    10861 cgtcgaacac cgccatcttg tcgcggccga cgaccgtgat gcgccgaatc ttgtgtgggt
    10921 cgagccagga cagatgcaga tgcgcgatct tgccggacgg aaagcggagg taacaaaaca
    10981 cgatgtcctc gacgttcggg ttgaggaacg cgtggccgtg cgcccatagc tcggtcggct
    11041 cctcgccgat caggtagagg agcacggata ggtcgtgcac cccgagcgac cagagcgcgt
    11101 tctcgtgcgt ccggatcgtg ccgaggttct gcctgttgcc gtagacgcag agaacgtctc
    11161 cgaggtcgcc ggagtcgacg atctccttca gcttctgcac gccggggtgg tagaggagca
    11221 agtggcccgg catcagaaca cggccgcgct cctcggcgag atcgcagagc tcttccatct
    11281 cggcgccacg catagcggga ggcttctcga cgaacacgtg cttgcccgct tccaagctgc
    11341 gcctggcgag ctcgtggtgc gtcggcaccg gggtcgcgac gacgacggca tcgaccgagt
    11401 cgtccgcgag cacgtcttcg aagtcgccgg tcatcgtcgc ggtcgggtat cgctgcgcaa
    11461 gttcggcgcg ccgctcgtcg tcgatgtcgc agagccaggc gaggtcgccg agctcgtcga
    11521 agttgcgggc gaggttcttg ccccactggc cgagcccgac ttgtgcgatt cgagtggttc
    11581 gagccacact cacagtttcc acactttgcc gttgccggtg ccgttcctac cggttgcgtt
    11641 ccggagatcg accacgacct gcgcgcgctc gaccaggtcc tcgtagtcga tggagctgtg
    11701 cgccgtgacg atcacgacgc agtcgtagct ctcgggcgcg tagtcgaccg agcgcagacc
    11761 gtcgaactcc ggaacgtgag ggtcgtggta cgagacgtcg gtgccggcct tgtgcaggag
    11821 ctcgacgatc ttcttcgccg gcgactcgcg cacatcgtcg atgtccgcct tgtacgaaac
    11881 gccgaggatc aggatccgag agcctttgag cgacttctct gcgccgtggt tcagagcctg
    11941 cgagatcagc gagcggcaga agaacggcat gttggcgttc accttgccgg ccaggtcgat
    12001 gaactcggtg tagaagtcgt actcgcgcgc cttccaggtg aggtagaacg gatcgatcgg
    12061 gatgcagtgc ccaccgagtc ccggtcccgg cttgaagctc atgaacccga acggcttggt
    12121 ctcggccgcc tcgacgacct cccagacgtc cacctccatg cgctcacaaa gctgcgcgag
    12181 ctcgttgacg agagcgatgt tgactgcccg gaagatgttc tccagcagct tcgtcatctc
    12241 cgccgtctcc ggcgaggaga gcggcacgac ggtgtcgacc gcggagcggt agagctccac
    12301 cgcccgctcg gtacaggtcg gcgtgagacc gccgacaagc ttgggtgtgt tcttcgtcgt
    12361 ccaatcggtg cggcccggat cgacacgctc gggggagaag gccagatgga agtcctcgcc
    12421 ggccttcatg ccgctaccct cgagaatcgg ttgcaggctc tcgcgggtcg tgcccggata
    12481 ggtcgtcgac tcgagcacca cgatctggcc cttgcgaagg cgcggcgcga tctcggcggc
    12541 tgcgctctcg acgatggaga ggtccggctc tcggtgactc gacagcggcg tcggcaacgc
    12601 gatcaggatt gcgtccacct cttggagtgc gtcgtagtcg ctcgtcgcca aaacccgacc
    12661 ggcgtcgacg agagtcttta gggcctccga cggcacgtcg ccgatgtggc tttcgccgcg
    12721 gttgatcccg tcgacgacgt cctcgttcac gtccacgagc acaacgttct tacccgcctc
    12781 ggcaaagaca cgtgcgagcg gcatccccac gtatccggca cccacgatcg cgacgtccga
    12841 cttcatcagc gcacaaggta cttgggggaa gccggcgcta cactacgcac ccgcccgggg
    12901 ccgttagctc agctggtaga gcaggggact cttaatccca aggtcgcagg ttcgatccct
    12961 gcacggccca tcgccgagcg aagtccggct cggctgcgcc gagccatgat gacttcgctc
    13021 ggcctctcgt ttcgatccgc ggccgaagcg gccgacgccg gcaagccggc atcggcggcg
    13081 gtggtgaatg aggaaaacgg gctttcgcag agtaatgtca gacagttcgc tcaacccgtg
    13141 gggggtttgc tctggcgact cacgtcgaaa ccggatttct agacgacgcc gaacggcgct
    13201 tcgctgagga tcttgggcgg ctgcggctcc tcctcgaggc ctcctcgacg ctcctcggct
    13261 cgctcgacat cgacgcgatg ctccccgaga tcctcgccct tgccggccgg acgctcgccg
    13321 ccgacgcgta ctccgtctgg cagtacgacg acgccactgg catctggaac gtcggcgcac
    13381 gcgtcgggtt gtcggacgag tatgtcgccg cggcgatggc ggcgatccgg ggcaacaccg
    13441 ccaccgtgtc cctcgacgcg ccaatcgtgg ccgaagacat cgaacggacc gcgtggctga
    13501 cgcccgagca ccggagcgcg cacgcagcag agaggacgcg ttcgatgatg gcgctcccac
    13561 tccactatgg cgaacgagtc ctcggaaccc tcgtcgtcta ctatcgcgaa ccgcgtagct
    13621 tcagcgaggc cgagaagagc acggcaatgc tcctggccaa ccttgctgcc gccgccatcg
    13681 gcaccgctga gctctaccag gcccagcgcc gcctcgcgga ggaccagcgc ttcgtcgccg
    13741 aggcgagcga gctcctcgca tcgtcgctcg actacgagac cacgctctcg aatctcgcaa
    13801 cgcttgccgt tcctcgtttc gccgactggt gcacgatcga catggtcggt gacgacggtt
    13861 cgattcaccg cctgaccgtg gctcatgtcg accctgagaa agtgcgctgg gcccaagagc
    13921 tcgccgaccg gtatccggca gacacgagtg cgccttacgg ggtcccgaac gtgatccgaa
    13981 cgcaggaacc agagctgctc agggaaatcc ccgacgacct gctgcgtgag gcgacagcgg
    14041 acacgccgga gctctacgcc atcctcgaag cgctcggcct taagtcctcg atgtgcgtgc
    14101 cgctcgtcgc gcgcgggcga gcgctcggcg cgatcacgtt cgtctccgcc gagagcggca
    14161 gacgatacga ggaatccgat ctcgcgaccg ctcgcgacct ggcgcgccgc gccgcgatct
    14221 cggtcgacaa cgcccgcctg ttccgcgagg cggagggctc gcgacgtgaa gcacacgagt
    14281 ccctcgccgt cgtcgacgcg gttttcgccg cagctccggt cggactggcg ttcatggata
    14341 cgagcttccg ctacgtccgg gtgaacgagg cgctcgcagc aatcaacggg ctcccgagcg
    14401 acgagcatta cggccgtacc ctccgagaag tgctcgggga ggacctcgcc tccgagatcg
    14461 agccgctcca caggcaggtg ctcgagagcg gcgtaccgat cctcgaccgc tacgtcgagc
    14521 aggcgctacc ggcagcaccg ggcgaaaccc gcaactggct cgtgggctac tacccggtcc
    14581 tcgacgccga cgaaaacaag atcggcgtcg gcgtcgtgat caccgacgtc tccgagcggg
    14641 agcaggcacg ggcagccgcc gagcgcgcgg gagcgcggct ggccgtgctc gccgaagcca
    14701 gccagacgct ggcatcaaca ctcgattacg agtcgaccct ggcaagcctt gcgtctctgc
    14761 tcgtgccgag gttcgccgac tggtatgccg tcgacatcgt cgacgagaac gggctcttcc
    14821 gacgcctcgc cgtagtgcac tgggatcccg cgaagtccga atgggccgac aagtcgcgcg
    14881 acctctatcc gcccgacctc gaggaggacg aggggacggg ccgagccgtg cgcacgggcg
    14941 aagccgtgct ctaccggacg atctccgacg agctcctcgc ctcctcgacg aaggacgccg
    15001 cacacaacga gatccttcac cagctcggca tggcgtccgc gatggtcgtc ccgctcacag
    15061 ccgccgggcg caccttcggc gggctcatgc tcgtctcctc ggaccccgct cgtctctacg
    15121 acgacgacga cctcgagttc gcgaagcacc tgggccgtcg cgccgcagtc gcggtcgaca
    15181 acgcacgcct ctaccgtgcg gccgaggaac gtgcgcgtgc ggccatcgtc gtcgagcacg
    15241 tcgccgacgg cgtcctcctc gtcgaccagg acgagatcat caggctctgg aacccggcgg
    15301 ccgagcacat caccggtctg gccgctccga agacggtagg ccggagcacg gccgaggtct
    15361 tctcaccgtg gaagacgatc gcggagctcg cagagcgcgg cgagccccgg ccggagacgc
    15421 agcctgtcga ggtcaacggc cgcgagctct ggctgtcgat caccggtgtc gggttcgagg
    15481 gcggcacggt cttcgcgttc cgcgacctga cggcggagcg cgccgtggaa aagctcaagt
    15541 cggacttcgt ctccaccgtg tcgcacgagt tgcgcacacc gctcgccgcg atctacggag
    15601 ctgccctgac actccggcgc gaggacgtcc cgctcggcga gccgcaacgc atgggtctgc
    15661 tcgaggtgat cgcgagcgag tccgaccgcc tggctcgcat cgtcaacgac atcctctggg
    15721 cgagccggct cgaatcggga acgatgcaga ccacgatcca gaagtgcgac ggcgtcgcga
    15781 tcgcgcgaag tgttgtcgac gcggcacgcc actacattcc tccaagcatc gagctcgcga
    15841 tcaaggcacc gaagaaggtg ccgctggtcg ccgccgaccc ggacaaggct cgtcaggttc
    15901 tgacaaacct cgtcgacaac gcacgcctct accgtgcggc cgaggaacgt gcgcgtgcgg
    15961 ccatcgtcgt cgagcacgtc gccgacggcg tcctcctcgt cgaccaggac gagatcatca
    16021 ggctctggaa cccggcggcc gagcacat
//
LOCUS       DASVEU010000017         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_598357,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000017 DASVEU010000000
VERSION     DASVEU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598357"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF225_00735"
     CDS             <1..311
                     /locus_tag="VF225_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143807.1"
                     /translation="TAQEYTTLGFNVAERSSEGHPRVEVGGAYVEFHPGDPGGAEKPL
                     LNHVAVLVDEVEPVITEAEERDIVDNVVDAANTRAVFVWGPERVRIEYVEHKPGFALT
                     "
     gene            314..1363
                     /locus_tag="VF225_00740"
     CDS             314..1363
                     /locus_tag="VF225_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7143808.1"
                     /translation="MAGAGMAGLSAAARARELGATPVVYEKGTRPGGSMLLSSCVIWR
                     FREWDDFRAECPNGDEALQRVVWERLDDAIAWLESLGAPVVEHETGNPRTTGKRFDPR
                     GLTDTLVRAAGDVRLGQTGAEPAILATGGFQGNAELVAEYIHPGGSLRLRANPWSTGD
                     GLRHALARGAELSVGLEEFYGRNMPDADFSEGEFVPLAQLYGRFARVYNERGDEFFPG
                     EVSWSENDLVQATAQQPGARAWYVLDDDALAQSVRERKVADIVALAPTRVDPAELPFA
                     TPPEARVAVRVRPGITHTIGGLRVDERARVMGTDGLFAAGADAGGISTGGYASGLAAA
                     LVFGRISAESALDRV"
     gene            complement(1532..1726)
                     /locus_tag="VF225_00745"
     CDS             complement(1532..1726)
                     /locus_tag="VF225_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143809.1"
                     /translation="MTNGAGKHLLFVWKPSGYELREADGDAPAVGSSVEVDDREEQVI
                     KIGPSPLPNDPRPCAYLQAV"
     gene            complement(1739..2878)
                     /locus_tag="VF225_00750"
     CDS             complement(1739..2878)
                     /locus_tag="VF225_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7143810.1"
                     /translation="MAFAAAAIAALVALAVDSRALTLAALAVAIALAGVVAAFLIVSE
                     RRRHEAAELELTSEARFLESLVETMGSIAGGAEVLERTRQEAERLFEARALLLAPGER
                     PRQAPAENAIVIPLRAHEEEIGALRLTRQRPFDRDDVVRATVLADFAAREHENAELLD
                     DARVREAERSRLSDQLITAEQDERRRLANELHDGAVQSLSGVALLLDAGLNSIEEGRG
                     EEAQQIIRRALERHRATIGELRNLSFNLEPVVLRDQGFAPAVRALTDQIELAHNVRVE
                     LDVEAAEQLAAQTQAAMYQIIREALDQAVHRGPPQSLSVTMRETADGGVETAIADDAP
                     GERRRRSLEELEERARTLNGVLTVDQGEEAGTTVRVVLPPYATAR"
     gene            complement(2895..3533)
                     /locus_tag="VF225_00755"
     CDS             complement(2895..3533)
                     /locus_tag="VF225_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7143811.1"
                     /translation="MSTPSTPSEITCLIVDDHEVVREGLRLSLSRAPHIRVIGEASDG
                     ASAVALAERRRPDVVIMDVRMPGMDGLEATKILTERVPESAVLIFTAYSERSLLGRGL
                     ESGAKGYILKEAPHQTLLRAIEKVAGGEGYVDPALMPAFLTGKDKDEMLTGREREILQ
                     LLADGMSNQDVAAKLFISQETVKSHVRHILAKLEADTRTQAVAIALREAIID"
     gene            complement(3615..4514)
                     /locus_tag="VF225_00760"
     CDS             complement(3615..4514)
                     /locus_tag="VF225_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7143812.1"
                     /translation="MRIEYREEPCKSALNPVKGMGFKWSLNPYMGCVHRCTFCYVRAF
                     EKRSDRPSDDRYGTSIRVKTNVADVLRRELARPTWRHEQVTVGAATDPYQPVEGKYKL
                     TRACLEVFRDAANPFGIITRGPMIVRDIDVLVEASQRADVSVTFSIPTLDDEVWKRTE
                     PSTAHPHQRLKAVTALVEAGVKASVGMAPILPGISDRPEQLREVVRAAREAGATGIWA
                     NLLFLRPGTRAHFLEHLAEDWPEQLPLYERLYARRRAYLGSEERNPLRAEIGALAREY
                     GIRDRRAVKLAPPPEPEQLSMSF"
     gene            4513..5442
                     /locus_tag="VF225_00765"
     CDS             4513..5442
                     /locus_tag="VF225_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7143813.1"
                     /translation="MSSAGSIEQAFVQLEDGIQRLTLPLPTGPKHVHCYLAHGTLFDT
                     GLGLGEAPWESTDAERIAITHFHPDHVGGAQAAAEATGARVFQGGLDYAQCERVWGSD
                     DWPERIAAWFVRHGVPAAVVEDLIAQGHAYAAFIRYAIDPELLYEGSEVGGWQVLELP
                     GHADGHLGFLRDGILIGGDHLLRRITPAVGLYPESRPDPLGDYLVSLERTIDLAPRIV
                     YPGHGEPIEDAPMRARELIEHHRIRLDDHAAALAPEPRTAYEISLAVFGRELTPTQRR
                     FAVAETLSHLERLVREGRAARDGDDNLVSYTSL"
     gene            5490..>6032
                     /locus_tag="VF225_00770"
     CDS             5490..>6032
                     /locus_tag="VF225_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX7143814.1"
                     /translation="MTILFELVAVGALILLNAFFVAAEYGLVTARRTRIVELQHQGDR
                     RARDALRITSDPPRFIAAMQLGVTLTSLGIGALGEVALSRLFDKVIATVLAVVLAYLV
                     LTFLHVVIGELVPKGIALGHSEGTALFVAAPVRFFFIVMRPVVWFLEAATEIVLKWLG
                     LKPPGADDDVLSEAELRMLVS"
BASE COUNT          892 a         2089 c         2067 g          984 t
ORIGIN      
        1 agaccgcgca ggagtacacg acgcttggtt tcaatgtcgc cgagcgatcc agtgagggtc
       61 atccgcgtgt cgaggtcggc ggcgcgtacg tcgagttcca tcccggcgac ccaggcggcg
      121 ccgagaagcc gctgttgaac cacgtcgccg tgctcgtcga cgaggtcgag ccggtcatca
      181 ccgaggctga ggagcgagac atcgtcgaca acgtcgtcga cgcggcgaac acgcgcgcag
      241 tcttcgtctg gggcccggag cgcgtacgca tcgagtacgt cgagcacaag ccgggattcg
      301 ctctcacgta gtcatcgcgg gggcggggat ggcgggactg tccgccgccg cgcgcgcacg
      361 tgagctcggc gcgacccctg tcgtgtacga gaagggaact cggccgggcg gttcgatgct
      421 gctgtcgagc tgcgtcatct ggcgctttcg cgagtgggac gactttcgcg cggagtgtcc
      481 gaacggcgac gaggcgttgc agcgtgtcgt gtgggaacgg ctcgatgacg ccatcgcgtg
      541 gctcgagtcg ctcggcgcgc cggtggtcga gcacgagaca gggaatccgc gcacaaccgg
      601 aaagcggttc gatccgcgcg gattgacgga cacgttggtt cgtgcggcgg gtgacgttcg
      661 cctcgggcag acgggagctg agccggcgat tctcgcgacg ggtggctttc agggaaacgc
      721 ggagctcgtc gccgagtaca tccatccggg cggctcgctc aggctgcgcg cgaatccgtg
      781 gagcacgggc gacgggctgc gtcatgccct ggcgcgcggc gcggagcttt cggtgggact
      841 ggaggagttc tacgggcgca acatgccgga cgcggatttc tccgagggtg agttcgtccc
      901 gcttgcgcag ctctacggtc ggttcgcgcg tgtctacaac gagcgcggcg acgagttctt
      961 tcccggcgag gtgtcgtggt ccgagaacga cctcgtgcag gccactgcgc agcagccggg
     1021 cgcgcgtgcc tggtacgtac tggacgacga cgcgctcgcg cagagcgtcc gtgagcgcaa
     1081 ggtggcggac atcgtcgcgc ttgccccgac ccgcgtcgat ccggctgagc ttccgttcgc
     1141 gacgccgccg gaggcacgag tcgcggtgcg agtacggccc gggatcacgc acacgatcgg
     1201 cggcctgcgc gtcgacgagc gagcccgggt gatgggcaca gacggactgt tcgccgcggg
     1261 cgccgacgcg ggcggcatct cgacgggcgg ctacgcgagc gggctggccg cggccctcgt
     1321 gttcgggcga atcagcgccg agagtgccct cgaccgcgtg tagcggttcc gtgacgggct
     1381 cgtcacagtt ccgtacccgt gcctggcacc ggtacgagcc cagggatgca gcaccgcctg
     1441 agcgatcgct gagttgcgtg acgcgctcgt cacacgggcg tacccgtgcc tggcaccggt
     1501 acgttcgcga ggagtcgtcc cctcgccaag gtcagacagc ttgtaggtat gcgcacggcc
     1561 gcggatcgtt cggcagcggc gaggggccga tcttgatcac ctgctcctcg cggtcgtcga
     1621 cttccacgct cgagccgacc gccggcgcgt cgccgtcagc ttcgcgtagc tcgtagccgc
     1681 tgggcttcca cacgaagagg agatgcttgc cggcgccgtt ggtcatcggc cgcggatcct
     1741 aacgagcggt tgcgtacgga gggagcacga cgcggacggt cgttccggct tcctcgccct
     1801 ggtcgacggt tagaaccccg ttcagcgtcc gcgcccgctc ttccagctct tcgaggctgc
     1861 ggcgccggcg ctcgccgggc gcgtcgtccg cgatcgcggt ctccacgccg ccgtccgctg
     1921 tctcgcgcat ggtcaccgac agcgactgtg gcggcccgcg gtgcaccgcc tggtcgaggg
     1981 cctcgcggat aatctggtac atcgccgcct gggtctgcgc cgcgagctgc tcggccgcct
     2041 cgacgtcgag ctcgacgcgg acgttgtgcg cgagctcgat ctgatccgtc agggcgcgga
     2101 ccgcgggcgc aaagccctgg tcgcgcaaga caaccggctc gagattgaaa gagaggttcc
     2161 gcaactcgcc gatcgttgcg cgatggcgct cgagcgcgcg gcggatgatc tgctgcgcct
     2221 cctcgccacg accttcctcg atcgagttca accccgcatc gagcaagagc gcgactcctg
     2281 agagcgactg caccgcaccg tcgtgcagct cgttggcaag gcgtcgccgc tcgtcttgct
     2341 cggccgtgat gagctgatcg gacagccgcg agcgctcggc ctcgcgcacg cgcgcatcgt
     2401 cgagcagctc ggcgttctcg tgctcgcgcg cggcgaagtc ggccagcacg gttgcgcgta
     2461 cgacatcgtc acgatcgaag ggacgctggc gcgtcaaccg cagcgcgccg atctcctcct
     2521 cgtgcgcccg caggggaatc acgatcgcgt tctccgccgg cgcctgccgc ggccgctcgc
     2581 ccggcgcgag cagcagagcg cgcgcctcga agagacgttc cgcctcctgg cgggttcgct
     2641 ccaacacctc ggcgccaccc gcaatcgacc ccatcgtctc gacgagcgac tcgaggaagc
     2701 gcgcctcgct cgtcagttcc agttccgctg cctcgtgtcg ccggcgctcg ctcacgatca
     2761 ggaacgccgc cacgactccc gcgagcgcga tcgccaccgc gagggcggcg agcgtcagcg
     2821 cacggctgtc cacggccagc gcgaccagag ccgcgatggc cgccgcggcg aacgccaaga
     2881 ccagcagtcc ggctctagtc gatgatcgct tcgcgaagcg caatcgcaac cgcttgcgtc
     2941 cgcgtgtccg cctcgagctt ggcgaggatg tggcggacat ggctcttcac cgtctcctga
     3001 ctgatgaaga gcttcgcagc gacgtcctga ttggacatgc cgtccgcgag cagttggagg
     3061 atctcccgct cgcgcccggt cagcatctcg tccttgtcct tgccggtcag gaacgcgggc
     3121 atcagcgccg ggtcgacata gccttcgccc cccgcgacct tctcgatcgc acgcaacagc
     3181 gtctggtgcg gcgcctcctt gaggatgtag cccttcgcgc cggactcgag cccgcgtccg
     3241 agcagcgacc gctcgctgta tgccgtgaag atcagcacgg ccgactccgg gaccctttcc
     3301 gtcaggatct tcgttgcctc gagcccgtcc atcccgggca tgcgcacgtc catgatcacg
     3361 acgtcaggcc tgcgccgctc ggccagggca acggcgcttg ccccgtcgga cgcctcgccg
     3421 atgacgcgga tgtgcggcgc gcgcgaaagc gacagccgta ggccctcccg gacgacctcg
     3481 tggtcgtcga cgatcaggca ggtgatctct gagggggtgg agggggtgga cacccgtgcg
     3541 gatgatattc ggcccaggcg tcgacgcagg cgacgccgcg taggaggccg tgcgggcttt
     3601 gcccgtgcgg ccgttcaaaa gctcattgaa agctgctcgg gctccggagg aggggcgagc
     3661 ttgacggccc gacggtcccg gatcccgtat tcacgcgcga gcgcgccgat ctcggcacgc
     3721 aacggatttc gctcctcgga gccgaggtag gcgcggcgcc gcgcgtacag ccgctcgtag
     3781 agcgggagct gctcgggcca gtcctcggcg agatgttcga gaaagtgcgc ccgcgttccg
     3841 gggcggagaa agaggagatt ggcccagata ccggtcgccc ccgcctccct cgcggcccgc
     3901 acgacctccc gcagctgctc gggccggtcg gagatcccgg gcaggatcgg cgccatcccg
     3961 acgctcgcct tcacgccggc ctcgacgagc gccgtcacag ccttcagccg ctggtgcgga
     4021 tgcgccgtag aaggctcggt gcgcttccag acctcgtcgt cgagcgtcgg aatcgagaac
     4081 gtgacgctca cgtccgcgcg ctgtgacgcc tcgacgagca cgtcgatgtc gcggacgatc
     4141 atcggcccgc gcgtgatgat cccaaacgga ttcgccgcgt cgcggaacac ctcgaggcac
     4201 gcccgcgtca gcttgtactt cccctcgacc ggctggtacg ggtcggtcgc ggcgccgacg
     4261 gtcacctgct cgtgccgcca cgtcggtcgc gcgagctccc gccgcaacac gtcggccacg
     4321 ttcgtcttca cgcggatcga cgtcccgtaa cgatcgtccg aaggccggtc cgaccgcttc
     4381 tcgaaggcac gcacgtagca gaaggtgcag cggtggacgc agcccatgta cgggttcagc
     4441 gaccacttga agcccatgcc cttgaccggg ttgagggcgc tcttgcaggg ctcttcgcga
     4501 tactcgatcc gcatgtcctc agcaggcagt atagaacagg cgttcgtaca actcgaagac
     4561 ggtattcagc ggctgacgct gccgctcccg accgggccga agcacgtcca ctgctacctg
     4621 gcccacggca cgctcttcga caccggcctc gggctcggag aggccccgtg ggagtcaacg
     4681 gacgcagaac gcatcgcgat cacccatttc caccctgacc acgtcggcgg agcgcaggcg
     4741 gcggcagagg cgaccggcgc tcgggtcttc cagggcgggc tcgactatgc ccagtgcgag
     4801 cgggtgtggg gaagcgacga ctggccggag cggatcgcgg cctggttcgt ccgacatgga
     4861 gtgcccgccg cggttgtcga agacctcatc gcacaggggc acgcgtacgc cgccttcatc
     4921 cgttacgcga tcgatcctga gctgctgtac gaaggcagcg aggtcggagg ctggcaggtc
     4981 ctagagctgc ccggtcatgc cgacgggcac ctgggcttcc tccgcgacgg catcctgatc
     5041 gggggcgacc acctgctgcg gcggatcacg ccggctgtcg gcctctaccc cgagagccgc
     5101 ccggacccgc tcggcgacta cctcgtctcg ctcgagcgca cgatcgacct cgcgcctcgc
     5161 atcgtgtatc cgggccacgg tgagccgatc gaagacgcgc ctatgcgggc tcgcgagctg
     5221 atcgagcatc accggatcag gctggacgac cacgcggcag cgctcgcgcc cgaaccccgt
     5281 accgcctacg agatctcgct ggctgtcttc gggcgggagc tcacgcccac ccagcgccgt
     5341 tttgccgtcg ccgagaccct gtcccacctg gaacggcttg tccgtgaggg acgcgcggcg
     5401 cgggacggtg acgacaacct cgtttcctat acttccctct agttggacga cgaaatcccg
     5461 cctagtacca ccgcccacgg ggtcggggcg tgacgatcct cttcgagctc gtcgccgtcg
     5521 gcgctctgat cctgctgaac gccttcttcg tcgcggccga atacggcctc gtcaccgcgc
     5581 ggcggacccg catcgtcgag ctgcagcacc agggcgaccg gcgggcgcgc gacgcgctgc
     5641 gcatcacctc cgacccgccg cgcttcatcg ccgcgatgca gctcggagtg acgctcactt
     5701 cgctgggcat cggtgcgctg ggcgaggtgg cgctgtcgcg gctcttcgac aaagtgatcg
     5761 ccacggtcct cgccgtggtg ctcgcctacc tggtcctcac cttcctccac gtggtgatcg
     5821 gcgagctcgt gccgaagggg atcgcgctcg gacactccga aggaacagcg ctgttcgtag
     5881 cggcgccggt gcgtttcttc ttcatcgtca tgcggccggt cgtgtggttc ctcgaggccg
     5941 cgacggagat cgtcctgaag tggctcggcc tgaagccgcc gggcgcggac gacgacgtgc
     6001 tctcagaggc ggagctgcgc atgctcgtct cg
//
LOCUS       DASVEU010000018         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_987588,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000018 DASVEU010000000
VERSION     DASVEU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987588"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF225_00775"
     CDS             complement(<1..793)
                     /locus_tag="VF225_00775"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX7143815.1"
                     /translation="MAKVLCVLYEDPVDGYPSSYARDAIPRIERYHDGQTTPAPERID
                     FEPGELLGSVSGELGLRRFLEERGDAFVVTSDKDGPDSVFERELPDAAIVISQPFWPA
                     YLTAERIAKAPGLKLAVTAGVGSDHVDLEAAIERGITVAEITYSNSISVSEHVVMMIL
                     SLVRNYIPSYQWVVKGGWNIADCVARSYDLEGMQVGTVAAGRIGSAVLRRLKPFEVGL
                     HYTDRHRLPEEVERELGVTYHPDAESMVRVCDVVTINAPLHPETEH"
     gene            1690..>2477
                     /locus_tag="VF225_00780"
     CDS             1690..>2477
                     /locus_tag="VF225_00780"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143816.1"
                     /translation="MDRPIGILYEHPLWFEPLFAELDRRGVDYRRLPAGELIFDPGER
                     RSPYSVVVNRMSPSAWTRGHSHAIFHTLHYLAYLDQIGANVVNGHAAFQVEISKARQC
                     ALFARLGLRYPAARAVNEPAQVSAAAEGLRFPVLVKPNVGGSGAGITSFETPAELDQA
                     AAADTLELGLDGTALVQEHLPAADEAVVRVEILGGKFLYAIRLRLLPGSFNLCPADYC
                     ELPGILDGVSGRGLPIERFEPPDGVVEDAKRIVEAAGMELGGVE"
BASE COUNT          392 a          859 c          805 g          421 t
ORIGIN      
        1 ggtgctcggt ctcaggatgg aggggcgcgt tgatcgtgac cacgtcgcag acacgcacca
       61 tcgactcggc gtccgggtgg taggtcacgc cgagctcgcg ctcgacctcc tccgggagtc
      121 ggtgtcgatc ggtgtagtgc aatcccacct caaacggctt cagccgcctg agcacagcag
      181 aaccgatccg gccggcagcg actgtcccca cttgcattcc ttcgaggtcg tatgacctgg
      241 cgacgcagtc ggcgatgttc catccgccct tgacgaccca ctgataagac gggatgtagt
      301 tgcgcaccag cgacaggatc atcatcacca catgctcgga cacgctgatg ctgttggaat
      361 aggtgatctc ggcgacggtg atcccacgct cgatcgccgc ttccagatca acgtggtccg
      421 agccgacccc cgccgtgaca gcgagcttca gccccggcgc cttcgcgatc ctctcagccg
      481 tcaggtaggc aggccagaac ggctgggaga tgacgatcgc agcgtcaggg agctctcgct
      541 cgaagaccga gtccggcccg tccttgtccg acgtgacaac aaacgcgtcg ccgcgctcct
      601 cgaggaaccg ccgcagccca agctccccgg aaacgctgcc cagaagctca cccggctcga
      661 agtcgatccg ctcgggggcc ggcgtagtct gaccgtcgtg gtagcgctcg atcctgggaa
      721 tcgcgtctcg agcgtacgag ctcggatacc cgtcaacagg atcctcgtac aggacacaga
      781 gaacctttgc cacctctgcc tcaccttcct cgcgtccgcc ctggcatcgc ctgcggattc
      841 tagacctctc cgtgagcgca gctcgcgggg ttggaagggc agttcggcgg tgaaggtcga
      901 gggcctcgaa gcggaaagca cccgcatttt gccgaccctg aggccgtagt cgtcggcgag
      961 ctcggcctgg acccgcggcg cgccgcagct ctcctgcgag gcgctgaaga tgcggcggat
     1021 cagtgcctgg agcttcgcgt cttcgcgctt acgaggcgaa gggcagcgct ccctccaggc
     1081 gtagtagtcg gctcgggtga cgccgagcac ggcgcacaac agggagacgg gggacaagct
     1141 ctgaatcgct cgagccgcag ctcagtaaat ttccttcctg attagggcgg gaccgccccg
     1201 tcgcgcacga tcggggtgcc cttccgagga gcggacacac cgcggtccat cggccgcgcc
     1261 tcccttgcgt caaccgcgtg agcggtgggc tcctcgtcgc cgcgggtgcc tactcgtcta
     1321 ctacttcggt ctctgggcgg tgggcgtgcg ccggtccgag accgatcgac tcgaggaagg
     1381 cgcgtgttgt ctgcacgggc gttcctgagc ttgtcggcgg gtcgatgtaa acggtcttcc
     1441 actcggcgaa gaagtcgcgt gccctgctcg cgcgatccgc tcgagctgtc cttcatatcg
     1501 caaaaggggc ctggaactcg agcccggccg tctcctggtt caccttcacg accccagccc
     1561 ggatctcgcg cgcgaactgc agcgccctcc cggacgtcgc ccgcgtccgg cgcagacaga
     1621 cggccgagct cccgtgcgtc acttcagttt ctatagcgat ccgtgctgca cgtctgtagg
     1681 ctcggggcga tggatcggcc gatcggcatc ctctacgagc atccgctgtg gttcgagccg
     1741 ctcttcgccg agctcgaccg acgcggggtc gactacagga ggctgcccgc cggtgagctg
     1801 atcttcgacc cgggcgaacg gcgaagcccg tactccgtcg tcgtcaaccg gatgagcccg
     1861 tcggcgtgga cgcgcggtca ctcgcacgcg atcttccaca cgctgcatta cctcgcgtac
     1921 ctggatcaga tcggggcgaa cgtcgtcaac gggcacgccg cgttccaggt cgagatctcc
     1981 aaggcgcgcc agtgcgcgct gttcgcgcgt ctcggcctcc gctacccggc cgcacgggcc
     2041 gtgaacgagc ccgcccaggt gtcggcggcg gcagagggcc tccgcttccc cgtgctcgtc
     2101 aagccgaacg tcggcggaag cggggccggc atcacatcct tcgagacgcc cgccgagctc
     2161 gaccaggccg ccgccgcgga caccctcgag ctcggcctgg atgggacggc gctcgtacag
     2221 gagcacctgc ccgcggccga cgaggcagtc gtgcgcgtcg agattctcgg cggcaagttc
     2281 ctctacgcga tccggctgcg actgcttccg gggagcttca acctctgtcc cgccgactac
     2341 tgcgagctgc ccggaatctt ggacggcgtc tccggccgcg gcctcccgat cgagcgcttc
     2401 gagccgccgg acggcgtcgt cgaggacgcc aagcggatcg tcgaggccgc gggcatggag
     2461 ctcggcggcg tcgagta
//
LOCUS       DASVEU010000019         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1571240,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000019 DASVEU010000000
VERSION     DASVEU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571240"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VF225_00785"
     CDS             complement(<1..273)
                     /locus_tag="VF225_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143817.1"
                     /translation="MRAAELDAVTLDAHGTLVKLVDPVPALVNALAERGVERIPEVVL
                     AGFQSEVAHYAPRAGEGHDEESLTRLQHECAGVFLDAVEAELDPEEF"
     gene            complement(270..1439)
                     /locus_tag="VF225_00790"
     CDS             complement(270..1439)
                     /locus_tag="VF225_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX7143818.1"
                     /translation="MRAGQRQALDIYLWSRAAIWVAAIFAFFFFEPNRHPDAARWDSA
                     RLHDLGYFTDIWGRWDSDFFLRIAQNGYGDASAAFHPLYPALIAGLGRLFLGHYLLAG
                     LVISLLCCLGSFVLLHRLAEERLGPDGAQRSVLYLAVFPMALFLQAVYSESLFLLLVL
                     AAFALAERNRFAGAGLVAGLAILTRAAGLALLPALALLAWRHRERLRSLAGIALAVPV
                     AAIYPLVLWQQVGDPWAFSDAQDRWHRHLSRAGPLGGIWDGLVAGWRGLEQFVVGHGT
                     NVPGANPMHAAAENVQALAFLALFLALTVVAWRRFGAPYGLFAAVSLAIPLAYPSARW
                     PLLSLPRFGLVIFPFFLALAAVTAAKPRLHTAVVACSALFLGVAVVQWALWQWVA"
     gene            1462..1863
                     /locus_tag="VF225_00795"
     CDS             1462..1863
                     /locus_tag="VF225_00795"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX7143819.1"
                     /translation="MPRTFTPEEANEALVELRPIVERMVEHRRKLAAAQLLQAELVTR
                     IAGNGGDMVPSDLHEAADAIQQEADAISDCAERINAVGAEVKSLEEGLLDFPAKRGDE
                     VVLLCWKLGEEEIHYWHRAGEGFAGRKPLPL"
     gene            1860..2426
                     /locus_tag="VF225_00800"
     CDS             1860..2426
                     /locus_tag="VF225_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7143820.1"
                     /translation="MRVGAFEVTALVDGEGSFSTVGEAFPALDAPEAWLLPVNAVLIR
                     GAGSTVLVDTGLGPEPRAFMPGAGARLLTELARVGSSAAEVDLVVHTHLHVDHVGWDG
                     SFPNARYVVPTDDWSYFMSEESLQQRPHLGDRVEPLGDAGLRARRRRRTSCSSPTRIS
                     STSATTIPACPPRRGSTCWGGSRMQART"
     gene            2531..3247
                     /locus_tag="VF225_00805"
     CDS             2531..3247
                     /locus_tag="VF225_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7143821.1"
                     /translation="MPFWHRLIVVGVVMFVTVAVARMIDRRIARRDLPPEAITRYRVV
                     RRSITSTIIFVGLLTALLVIPQVRAVAGGLLASSAVIGIVVGFASQRTLGNFVAGLLI
                     AFTQPVRLGDDVVIESTEGTVEEIGLIYTFVRTQNGDRLVIPNEKLASDTIRNSTIRS
                     REKVAEISLQVPLGKDLGAVVDRLRTIAADGDVFVGDLSGSATVVVRVPATNRSSAEQ
                     RARELRLQAYEVLRAEGVYA"
     gene            3244..>4201
                     /locus_tag="VF225_00810"
     CDS             3244..>4201
                     /locus_tag="VF225_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX7143822.1"
                     /translation="MTPPQRGRRSQSDTFLLDRLRKRRRQRVKNRRKRFGRLVSGALI
                     GTVLFLVVSSFTGAAVWMNSCSLDSLKPVDVGENSFVFAANGSLLGSIPAEQNRQPVG
                     LAQISKWAPAATIAIEDRRFYKHGALDWLGIVRALYADIQAGRVVQGGSTITQQLVRN
                     LYITEKSQTLGRKATEACLAIKLAREKSKTWILNAYMNQVYYGNHAYGIEAAAQTYFS
                     KPARKLSLTESALLAGLPQAPSVFDPFQRPAQAIARRDEVLRAMLANGNITSSRYADA
                     VAQRKLHLKAGRLYTRIREPYFFSYVREELQRQYGANTVRSGG"
BASE COUNT          727 a         1390 c         1471 g          613 t
ORIGIN      
        1 gaactcctcc gggtcgagct ccgcctcgac ggcgtcgagg aacacgccgg cgcactcgtg
       61 ttgcagtcgc gtcaggctct cttcgtcgtg accctcaccg gcgcgaggag cgtagtgcgc
      121 aacctcgctc tggaaccccg cgagcaccac ctccgggatg cgctcgactc cccgttcggc
      181 gagcgcgttc accaacgccg gaacgggatc caccagcttg acgagcgtgc cgtgcgcgtc
      241 gagcgtgact gcgtcgagct cggcggccct cacgcgaccc actgccagag tgcccattgc
      301 acgaccgcga ccccgaggaa gagcgcgctg caggcgacga ccgcggtgtg gagccgcggc
      361 ttcgctgcag taaccgctgc gagggcgagg aagaagggaa agatcaccag cccgaagcgc
      421 ggcaaggaga gcaacggcca gcgagcgctg gggtaggcca aggggatcgc gagactgacc
      481 gcggcgaaca ggccatacgg cgcgccgaac cggcgccacg cgacgaccgt cagcgccagg
      541 aagagggcga ggaacgcgag agcctggacg ttctcggcgg ccgcgtgcat cggattcgcg
      601 cctgggacgt tcgtgccgtg tcccacgacg aactgctcca ggccgcgcca gccggcgacg
      661 aggccgtccc agatcccacc cagcggcccg gcccgtgaca ggtgccggtg ccagcggtcc
      721 tgcgcgtccg agaacgccca ggggtcgccg acctgctgcc agaggacgag tgggtaaatc
      781 gctgcaacgg gaactgccag cgcgatgcct gccagagagc gcagccgctc ccggtggcgc
      841 cacgccagga gcgccagcgc cgggagcagt gcgaggccgg cagcccgggt caggatcgcc
      901 agtccggcga cgagcccggc accggcgaag cgattgcgct cggcgagcgc gaatgctgcg
      961 aggacgagca gcaggaacag cgactcgctg tagaccgcct ggaggaagag cgccatcggg
     1021 aagacggcga ggtagagaac gctgcgctgc gccccgtcgg gcccgagccg ttcctcggcc
     1081 agacggtgca ggagcacgaa cgagccgagg cagcagagca gggagatgac gagcccggcg
     1141 aggaggtagt ggccgaggaa caggcgaccg aggccggcga tcagcgccgg gtagagcggg
     1201 tgaaacgcag cggacgcatc gccgtagccg ttctgggcga tgcgcaggaa gaagtcgctg
     1261 tcccagcggc cccagatgtc tgtgaagtag ccgaggtcgt ggaggcgcgc cgagtcccag
     1321 cgggctgcat ccggatgccg attgggctcg aagaagaaga aggcgaagat cgccgcgacc
     1381 cagatcgccg cgcggctcca gaggtagatg tccagcgctt gcctttgacc cgcgcgcacg
     1441 gccgcatgat catacggacg aatgcctcgt accttcacgc ccgaagaagc caacgaggcc
     1501 ctcgtcgagc tgcggccgat cgtcgaacgg atggtcgagc atcgacgcaa gcttgcggct
     1561 gcgcagctcc tccaggcgga gctcgtgacg cgcattgccg gaaacggcgg cgacatggtc
     1621 ccgagcgacc tccacgaagc ggcggacgcg atccagcagg aggcggacgc gatctcggac
     1681 tgtgctgagc gcatcaacgc cgtcggcgcg gaggtgaagt cgctggagga agggctgctg
     1741 gacttccccg ctaagcgcgg cgacgaggtc gttctcctct gctggaagct cggcgaggaa
     1801 gagattcact attggcaccg ggccggcgag ggattcgcgg gacggaagcc gctgccgttg
     1861 tgagagtcgg cgctttcgaa gtgacggccc tcgtcgacgg cgagggaagc ttctcaaccg
     1921 tcggggaggc gttccccgcg ttggacgcgc ccgaggcgtg gctgctgccg gtcaacgcag
     1981 ttctgattcg cggtgccggt tcgacggtgc tcgtcgacac cgggctcgga ccggagccgc
     2041 gcgcattcat gccgggcgcc ggcgcaagac tcctgaccga gctggcgcgt gtcggctcga
     2101 gcgcggccga ggtcgatctt gtcgtgcaca cgcatctaca cgtcgatcac gtcggctggg
     2161 acggctcctt tcccaacgcc cgctacgtcg tgccgacgga cgactggtcg tatttcatgt
     2221 cggaagagtc gctgcagcag aggccccacc tgggcgaccg cgtcgagccg ctgggcgacg
     2281 ccggcctgcg tgctcggcga cgtcgtcgta cgagttgcag ctcgccgacc cggatctcgt
     2341 ctacttcagc gaccacgatc ccggcttgtc cgcctcgacg cggaagcacg tgctggggcg
     2401 gctcgcggat gcaggcacgg acgtgatcgt cagtcatttc cacgggcccg gccgcttcag
     2461 ccgcaacggc ggggggttca gctggtcgag ccgcgcaaag gacggcgggg ctgccgtcga
     2521 atagaggccc atgccgttct ggcaccgact gatcgtcgtc ggcgtcgtca tgttcgtcac
     2581 cgtcgcggtg gcgcgcatga tcgaccgacg catcgcccgc cgtgaccttc cgccggaggc
     2641 gatcacgcgc tatcgcgtcg tgcgccgcag catcacctcg acgatcatct tcgtcgggct
     2701 gctcaccgcc ctgctcgtga tcccacaggt gcgcgcggtg gccggcgggc tgcttgcttc
     2761 ctcggcggtg atcggcatcg tcgtcggctt tgcgtcccag cggacgctcg ggaacttcgt
     2821 cgccggcctg ctgatcgcct tcacccagcc ggttcggctc ggcgacgatg tcgtcatcga
     2881 gagcaccgag ggaacggtcg aggagatcgg gctcatctat acgttcgtcc ggacccagaa
     2941 cggcgaccgg ctcgtgatcc cgaacgagaa gctggcctcg gataccatcc gcaactccac
     3001 gattcgcagt cgagagaagg tcgccgagat cagcctccag gttccattgg gtaaagacct
     3061 cggtgcggtc gtggaccggc tgaggacgat cgccgcggac ggggacgtgt tcgtcggcga
     3121 cctgtccggc agcgcaacgg tcgtcgtgcg cgtgccggcg acgaatcgtt cttcggccga
     3181 gcagcgcgcg cgggagcttc gcctgcaggc ttacgaagtg ctgcgcgcgg agggcgtgta
     3241 cgcatgacgc cgcctcaacg cggacggcga tcacagtcgg acaccttcct gctcgaccgc
     3301 ctgaggaagc ggcggcggca acgcgtgaag aaccggcgga agcggttcgg caggctcgtg
     3361 tcgggagccc tcatcggcac ggtgctcttc ctggtcgtgt cgagcttcac aggcgcagcc
     3421 gtctggatga acagctgcag cctcgactcg ctcaaaccgg tggacgtcgg ggagaactcg
     3481 ttcgtcttcg cagccaatgg gtcgttgctg ggctcgattc ccgcggagca gaaccgacaa
     3541 ccggtcggac tcgctcagat cagcaagtgg gccccggccg caacgatcgc aatcgaggac
     3601 agacgtttct acaagcacgg tgcgctcgac tggctcggca tcgtgcgcgc gctctacgcg
     3661 gacatccagg ccgggcgagt cgtgcagggc gggtcgacga tcacccagca gctcgtccgg
     3721 aatctctaca tcacggagaa gtcgcagacg ctcgggcgca aggcgaccga ggcgtgcctc
     3781 gcgatcaagc tcgcccgcga gaagtcgaag acgtggatcc tcaacgccta catgaaccag
     3841 gtctattacg ggaaccacgc ttacggcatc gaggcggccg cccagacgta cttctccaag
     3901 ccggcgcgca agctcagcct gaccgagtcg gcgttgctgg ccggcctgcc gcaggcaccg
     3961 tccgtcttcg acccgttcca gcggccggcg caggcgatcg cccgcaggga cgaggtgctt
     4021 cgcgcgatgc tcgcgaacgg aaacatcacg tcgagccggt acgccgatgc ggtcgcccag
     4081 cgcaagctcc acctgaaggc cggacgtctg tacacgcgca tccgtgagcc gtatttcttc
     4141 agctacgtcc gcgaggaact gcagcgccag tacggcgcga acacggtccg ctccggcggg
     4201 t
//
LOCUS       DASVEU010000020         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_599867,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000020 DASVEU010000000
VERSION     DASVEU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599867"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VF225_00815"
     CDS             complement(<1..633)
                     /locus_tag="VF225_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143823.1"
                     /translation="MSTTESTHKLRDGAALPLRRVALAVPDVRVTRRGLQVVLGLFWL
                     LDGGLQFQPFMLRTSFARDVLAPTGSGQPQLVAGPVHWAANLIAAHPVAWDVPFASIQ
                     VLLGLGMLFPRTARLALAASLPWAAGVWLFGEGLSGLASGNTSLITGMPGSAVIYGVL
                     ALAAWPRRGRSDVPPARWLPLVWAAFWIGAAVLQVLPMNNTGADVSSALLA"
     gene            complement(721..2175)
                     /locus_tag="VF225_00820"
     CDS             complement(721..2175)
                     /locus_tag="VF225_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143824.1"
                     /translation="MSFNSYVRPRTLLAGVAALVAGVAVWLVLANGNAGAAGKAASTT
                     TTGTTAAPTTPPALGGSDTNLYPFGAPAGGVAIKVPAPPTEPTLSPAYTTKTTTRIYG
                     SDPFEEAVSVTQHIWPAVVPLNNPSENNNDPDRPWGVTLVTPDDPLTGITATPLIHFP
                     DDAPILYVTKNGIPEITLNELKRLGDTGISRFNNVDAFLVGAAANSGVEAKLKSIGLK
                     YVEVKGGDIPTLADNVDKLYGSIENPDTGVPTMPGYNVAIGSMDGKDWQYLLPATHWV
                     AHMPTGLLWVHTNSVPNSTIDALKRRGGMGNIYLFGGPNQISSAVAKQLAQYGRVVRV
                     TNDDPVAFNAPPKDSPEATAVAFAKYYDPMGMFGWKITGPGHGFSLVNVNDWQAAVAS
                     APLSHLGFHAPLILTSSATTLPSDLDAYFSSVSPFFLDSPADGPYNMTYVIGSWNQIS
                     WDQQARVDYISEMHNRRVVGSNTGSSYSDSQPGA"
     gene            2471..2683
                     /locus_tag="VF225_00825"
     CDS             2471..2683
                     /locus_tag="VF225_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143825.1"
                     /translation="MEPILISNAELVEYATDEELLIHAWCAEQLRRLGLRRVLAETFA
                     GLVDWHEIAALVARGCPPELALEIAR"
     gene            2683..3195
                     /locus_tag="VF225_00830"
     CDS             2683..3195
                     /locus_tag="VF225_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143826.1"
                     /translation="MSAATAVAPAQADSAKSFLLQRAQPAMTGLIDGSLSTLAPIFAV
                     ALATHRPQYAFFAGLATAIGAGVSMAFSEGLSDDGELTGRGNSYLRGSITGAGTFLGG
                     ILHTLPFLIPQYRAALIVAVVVIAFELTTLAWIRSRFFGSGFLRSFVSVALAGAIIAA
                     VSAALGGAAG"
     gene            3264..4292
                     /locus_tag="VF225_00835"
     CDS             3264..4292
                     /locus_tag="VF225_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7143827.1"
                     /translation="MNIIRKLPKRVVLLVVVALLAASVGAGIALAHGSAKPIGGGVVV
                     IDTNLAYQGNAAAGTGMVLTSSGEVLTNNHVISGATTIKVVVPKTGHTYKARVLGYDR
                     TADVALLQLQGPSNLKTVSTTSAKLSIGATVRALGNAGGNGSITTATGTVTGLGKSIT
                     ASDDAGGSEQLTGLIETNAGVQPGDSGGPLVNSKGQVVGMDTAASSRFGFQGISATDA
                     YAIPIAKAVAIAHAISSGKASSTVHIGATAFLGIEVEPNSPGFGGTSVSGALIAGVVT
                     GGPAASAGLVAGDVIIAISGHSVSSPAAVSALVLTKKPGAKITVAYVDASGASHSTSV
                     TLGSGPPQ"
     gene            complement(4300..4530)
                     /locus_tag="VF225_00840"
     CDS             complement(4300..4530)
                     /locus_tag="VF225_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143828.1"
                     /translation="MSENEQPSRIETFLYDADGNRTEDESVAVRGEVVELDAAGRVIA
                     RHAHDDLGWVVDPKSLEGDPGELATRPKHEAT"
     gene            4653..>5174
                     /locus_tag="VF225_00845"
     CDS             4653..>5174
                     /locus_tag="VF225_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143829.1"
                     /translation="MSTGGDPEQIRAMLATARGYAVFDREGTRMGAFIELAGAASDRI
                     AIRREGFFVWRRRLLPITTVAKVVPDKRAVVLTVEGRSLAGSGTRSASVPGTSGIAEE
                     DPRSNGDWRDRLERYVGPVEAQPAHADLSRGAEHEAPPNADEGGHPVAPAADQPEPDR
                     RDQRTADRHLLFVS"
BASE COUNT          828 a         1685 c         1784 g          877 t
ORIGIN      
        1 ggcgaggagg gcgctcgaga cgtcggctcc cgtattgttc atcggcagta cctgcaggac
       61 ggccgcgccg atccagaagg cagcccagac gagcggcagc cagcgagccg gtggaacgtc
      121 ggagcgaccg cgccgcggcc aggcggcaag cgcgagcact ccgtagatga ccgcagagcc
      181 gggcatgcct gtgatcaggc tggtgttccc gctggcgagg ccggataggc cctccccgaa
      241 cagccagacg ccggccgccc aaggcaggga cgcggcgagc gcgagccgcg cagtgcgcgg
      301 gaagagcatc cccagcccca gcagcacctg gatgctcgca aacggcacgt cccaggcgac
      361 cgggtgcgcc gcgatcaggt tcgctgccca gtgaaccggg ccggcgacga gctgaggctg
      421 accgcttccc gtcggcgcga ggacgtctcg ggcgaagctg gtcctcagca tgaacggttg
      481 gaactgcagg ccgccgtcca ggagccagaa gagccccagg acgacctgca agccgcgtcg
      541 cgtgactcgg acgtctggga cggcgagcgc gacgcggcgg agcggaagcg cggcgccgtc
      601 gcgcagtttg tgggtggatt cggtcgtgct catttcaagg tcctccgttg ggagtcgaat
      661 agtgtgcttc cggccgttcg gccggaagca cactgaggtg ggagtcgtgc ctgctagctc
      721 ctacgcgccg ggctggctgt cggagtagct gctaccggtg ttgctgccga ccacgcggcg
      781 gttgtgcatc tcggagatgt agtcgacgcg ggcctgctgg tcccagctga tctgattcca
      841 gctgccgatg acgtaggtca tgttgtacgg gccgtccgcc ggcgagtcga ggaagaacgg
      901 cgagacggac gagaagtacg cgtccaggtc ggagggcagc gtcgttgcgc tgctggtgag
      961 gatcaacggc gcgtggaagc cgaggtgcga gagcggcgcc gacgcgaccg cggcctgcca
     1021 gtcgttgacg ttgaccagcg agaagccgtg gcctgggccg gtgatcttcc agccgaacat
     1081 gcccatcggg tcgtagtact tcgcgaacgc gaccgcggtg gcctcgggcg agtccttcgg
     1141 aggagcgttg aaggcgaccg gatcgtcgtt cgtgacccgc acgaccctcc cgtactgggc
     1201 gagttgcttc gccaccgccg acgagatctg gttggggcca ccgaacaggt agatgttccc
     1261 catcccgccg cggcgcttga gcgcgtcgat cgtggagttc ggcacggagt ttgtgtgcac
     1321 ccagaggagc cctgtcggca tgtgcgcgac ccagtgtgtg gccgggagca ggtactgcca
     1381 gtcctttccg tccatcgagc cgatcgcgac gttgtaaccg ggcatcgtgg gcacgcccgt
     1441 gtcggggttt tcgatcgagc cgtacagctt gtcgacgttg tcggcgagcg tcgggatgtc
     1501 tccgcccttg acctccacgt acttcaggcc gatcgacttg agcttcgcct cgacgcccga
     1561 attggctgcc gcgccgacga ggaacgcgtc gacgttgttg aaccgcgaga tcccggtgtc
     1621 gccgagccgc ttgagctcgt tgagcgtgat ctcggggatg ccgttcttcg tgacgtagag
     1681 gatgggcgcg tcgtccggga agtggatcag cggcgtcgcc gtgatccccg tcagcggatc
     1741 gtcgggcgtg acgagcgtga cgccccacgg ccggtccgga tcgttgttgt tctcgctcgg
     1801 gttgttcaag gggacgacgg ccggccagat gtgctgcgtg accgagacgg cttcctcgaa
     1861 cggatccgat ccgtagatgc gggtcgtcgt cttcgtcgtg tacgccggcg agagcgtcgg
     1921 ctcggtagga ggcgccggca ccttgatcgc gacgccaccg gccggggcgc cgaacgggta
     1981 gaggttcgtg tctgacccac cgagcgcagg cggggtggtg ggtgccgctg tggtaccggt
     2041 tgtggtcgtc gaggccgcct tgccggccgc cccggcgttg ccgttcgcga gcacgagcca
     2101 aacggctacg ccggcgacga gcgccgccac gccggcaagc aaggtgcgcg ggcgaacgta
     2161 cgagttgaag gacatcgtgt tccctccttg tcagagttgc agtggcctcg caagaatcgc
     2221 cgcgtagtcc tcgtgcgtca gcacagcgac catccatctg ggcttggggc gagtacgcaa
     2281 attcgaacta cgaggtagcc gccactaccc ggcttccccg gaaactcgcg gctgcgcatt
     2341 gcaggtagca ggaagtcgca aggttccgct agcactgcgc gaagatcgct gctggcgtcg
     2401 atgcgggaga tgcttgtcgc cgcgatcgtt ccaccagccg aacaaacctc gattcgggag
     2461 ggagagaccg atggagccga tcctgatttc aaatgccgag ctcgtcgagt acgcgaccga
     2521 cgaggagctg ctgatccacg cctggtgtgc cgagcagcta cgccgcctcg gcctgcgccg
     2581 tgtcctcgcc gagacattcg cagggctcgt cgactggcac gagatcgcag cgctggttgc
     2641 acgcggctgc ccgccggagc tcgcactcga gatcgcgcgc tgatgtcggc cgcaacggcg
     2701 gtcgctcccg ctcaggccga ctctgccaag tcgttcttgc tccagcgcgc gcagccggcg
     2761 atgacagggc tcatcgacgg atcgctctcg acgctggcac cgatcttcgc cgtcgcgctc
     2821 gcaacccacc ggccgcaata cgcattcttc gccgggctgg caaccgcgat tggcgcgggg
     2881 gtcagcatgg ccttctccga gggactctcg gacgacggcg agctgaccgg tcgtggaaac
     2941 tcgtacctcc gaggctcgat caccggggcc ggcacctttc tgggcgggat cctgcacacg
     3001 ctgccgttcc tgatcccgca gtaccgcgcc gcgctcatcg tcgcggtcgt ggtgatcgct
     3061 ttcgagctca cgacgcttgc gtggatccgc tcgcgcttct tcggcagcgg gtttcttcgc
     3121 tcgttcgtct ccgtcgcgct tgccggcgcg atcatcgccg ccgtcagcgc cgcgctcggc
     3181 ggggcggcgg gttgagcaca agctcataaa ggtcttagca gccgttcatg aagctcttag
     3241 caacgccagg taccactaac ggcatgaaca tcattcgcaa acttccgaag agagttgtcc
     3301 ttctcgtcgt ggttgcactc ctcgccgcgt cggtgggggc gggaatcgct ctcgcgcacg
     3361 ggtcggcgaa gccgatcggc ggcggcgtag tcgtcatcga cacgaatctc gcctatcaag
     3421 gcaatgcggc cgccgggacg gggatggtgc tgacatcgtc gggcgaggtg ctgacgaaca
     3481 accacgtgat cagcggcgcg accaccatca aggttgtcgt cccgaagacg ggccacacct
     3541 acaaggcacg agttctcggc tatgaccgga ccgccgacgt tgccctcctt cagttgcagg
     3601 gtccttccaa tctgaagacc gtctcgacca cctcggcaaa gctctccatc ggcgcgacgg
     3661 tgagggcgct cggcaacgcg ggcggtaacg gcagcatcac cacagcgacc ggcacagtga
     3721 ccggtctcgg caagtcgatc acggcgtcgg acgacgcggg tggctccgag cagctcaccg
     3781 ggctgatcga gacgaacgca ggggtccagc cgggcgactc gggcggccca ctcgtcaaca
     3841 gcaagggcca ggtcgtgggc atggacacgg cggcgtcgtc gcgattcggg ttccagggca
     3901 tctccgcaac ggacgcgtac gcaatcccga tcgcgaaagc ggtggcgatt gcccacgcga
     3961 tttcttcggg taaagcgtcc tcgacagtcc acatcggcgc gacggcgttc ctcggcatcg
     4021 aggtcgagcc caacagtccg ggcttcggcg gcacgtccgt gtctggtgct ctgatagccg
     4081 gcgtcgtgac cggcggtccg gccgcttccg caggccttgt cgcgggcgat gtgatcatcg
     4141 caatcagcgg ccactccgtc tcgtcgcccg cggcggtctc ggcgctcgtg ctcacgaaga
     4201 agcccggcgc gaagatcaca gtcgcatatg tcgatgcgtc gggtgcgagt cacagcacga
     4261 gcgtcacgct cggcagcgga ccgcctcagt gacgagccat caggtcgctt cgtgcttcgg
     4321 gcgggtcgcg agttcgcccg gatcgccctc gagcgacttt ggatcgacca cccacccgag
     4381 atcatcgtgt gcgtgcctcg cgatgacgcg gcccgccgcg tccaactcga cgacctcgcc
     4441 gcgcaccgcc acgctctcgt cctccgtgcg gttgccgtcg gcgtcgtaaa ggaacgtctc
     4501 gattctgctt ggctgctcgt tctcgctcat cgctcaccct ccattcgtag tgaactccgg
     4561 ttgcggtggt tccctacctc taccccaaac gttcggagcg gactccgcag tcctcgccag
     4621 agcgtcggct cacgcggagt acgcttgacg cgatgtcgac agggggcgat ccggagcaga
     4681 ttcgagcaat gctcgcaacg gcgcggggct acgcggtgtt cgaccgggag gggacgcgca
     4741 tgggggcgtt catcgaactc gccggcgcgg cgagtgaccg aatcgcgatc cgtcgggaag
     4801 gtttctttgt ctggcgtcgg cgtctgctgc cgatcacaac cgtggcgaag gtcgttcccg
     4861 acaagcgggc cgtcgtgctg acggtagagg gacggtcgct cgccggctcc gggacgcggt
     4921 ccgcctcggt ccccggaacc tccgggatcg cggaggaaga tccgcgttcg aacggtgact
     4981 ggcgagaccg actcgagagg tacgtcggac cggtcgaggc ccaaccagct catgcggacc
     5041 tcagccgcgg cgcggaacac gaggcgccgc cgaacgccga cgaaggggga cacccggtcg
     5101 cgccggccgc cgatcagccg gagccggacc gaagggatca gcgcaccgcc gaccgacacc
     5161 tgctcttcgt ctcg
//
LOCUS       DASVEU010000021         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_990256,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000021 DASVEU010000000
VERSION     DASVEU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990256"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF225_00850"
     CDS             <1..197
                     /locus_tag="VF225_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143830.1"
                     /translation="TDEPVRNFDDAQRSDTERYGALFRHLLERGIYIAPSQFEAMFVS
                     LAHGDEEIDRTIQAVADLDG"
     gene            178..714
                     /locus_tag="VF225_00855"
     CDS             178..714
                     /locus_tag="VF225_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143831.1"
                     /translation="MPISTADLWDTIAADAGQESALWTAALRPREEQERNPVFSRLGE
                     PRYALGIETIYEGYLLHYGRPRLFDPDDQDAALLLGDYLYAHGLVRVEEGGTVEAVSD
                     LAELIALCAYLRAEEIAGDGPVWAATAAALGRGELDDGRSCLRLDNDPGPLERLARTR
                     AGDAAVESALATHRARLS"
     gene            725..874
                     /locus_tag="VF225_00860"
     CDS             725..874
                     /locus_tag="VF225_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143832.1"
                     /translation="MLALLQLLAVEDAATEGKKVITGMLIVGLIFIGVIVLGETSHWL
                     RHRRR"
     gene            907..3351
                     /locus_tag="VF225_00865"
     CDS             907..3351
                     /locus_tag="VF225_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7143833.1"
                     /translation="MAELPTGTVTFLFTDVEGSTRLLHELGDAYADALGKHRKTLREC
                     FMRHGGVEVDTQGDAFFVAFARASDALAAAADGQAALEPGPIRVRIGLHTGEPLVTDE
                     GYVGIDVHRAARIAAVGYGGQILVSQSTRDLARAHGLRDLGHHRLKDLTAPERIYQFG
                     DDDFPPLKSLNTTNLPVASNPLVGRETELAELAAILMDSERLVTLTGPGGSGKTRLGL
                     QVGAELLEHFPGGVFFVPLAALSAPELVRPAISRTTGVPRLEELAGRRALLLLDNFEH
                     LLGAAPDVAALLEAGDSAKVLATSRAPLRVHGECEFAVDPLPESDAVELLVQRARSVR
                     RDFAPDEHAKEICRRLDGLPLAVELAAARLRSLDSAALLDRLDRRLPLLTGGARNAPE
                     RQRTLRATIEWSYDLLPDDLSALFARLAVFAGTFSLDAAEQVAGCTVETLDALVEASL
                     VKALAGNRFLMLETIRELALERFAELDDVAELRARHARYYHSVALSACLDTDVPDEQR
                     PEIVFPEAPNLRVALAWALETSETDFGLDLLVALEQFWVLGYTTEAMRWFTAFLEHAD
                     GAPPLLRARALRSFGSSAHFAGEFEFAERLCEESLAEYEQLGDEHGIAVLLHRLSIFA
                     LHRGDIEQASALSERSLELHRLLANDKGAAQPLALLGAIALQSGDRERGRALLEESAE
                     LAGKIRWRWWRAGTLSALAEVAIADDRIADAQGLLHEAVDLALQFGDRVGLSWYLSQY
                     ALTLLHDSRTEDAGRVWGTVEAAAAFVPGGPWPRDLDRLQHEVLALADAAFETGREAG
                     GSLSLEEAATRMCAID"
     gene            3356..>3639
                     /locus_tag="VF225_00870"
     CDS             3356..>3639
                     /locus_tag="VF225_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX7143834.1"
                     /translation="MAPPADLRAWIALLEREGELVRIAAEVDPDREITEITDRTVKAG
                     GPALLFENVKGSSHPLLINQFGTERRMCMAFDAPSLDSVATKLSDVLEMQP"
BASE COUNT          550 a         1255 c         1212 g          622 t
ORIGIN      
        1 tgaccgacga gcccgtgcgc aacttcgacg acgctcagcg ttcggacacc gaacgctacg
       61 gcgcgctctt ccgccacctc ctcgaacgtg ggatctacat cgctccgagc cagttcgagg
      121 ccatgttcgt ctcgctcgcc cacggcgacg aggagatcga ccgcaccatt caggccgttg
      181 ccgatctcga cggctgatct ctgggacacg atcgccgcgg acgcggggca ggagagcgcc
      241 ctgtggacgg ccgccttgcg cccgcgcgag gagcaagaac gaaatccggt cttctcccga
      301 ctcggcgaac cgcggtacgc gctcgggatc gagacgatct acgaggggta cctcctgcac
      361 tacggccgcc cgcgcctgtt cgacccggac gaccaggacg cagcgctgct cctcggggac
      421 tacctctatg cacacggcct tgtgcgcgtc gaagagggcg gcacggtcga ggcggtcagc
      481 gacctcgccg aactgattgc gctctgcgcg tacctgcgtg ccgaagaaat tgctggtgac
      541 gggccggtgt gggcggcaac tgccgcggcc cttggccgtg gcgagctgga tgacggccgg
      601 tcgtgtcttc gcctcgacaa cgatcccggt ccgctcgagc gtctcgcccg gactcgggcg
      661 ggcgacgctg ccgtcgagag tgcactggcg acccatcgcg cgcgcctgag ttagagtcac
      721 gccgatgctt gcgctgctcc agttgctcgc agtcgaggat gccgcgaccg agggcaagaa
      781 ggtcatcacg ggcatgctga tcgtcggcct gatcttcatc ggcgtgatcg tcctcggcga
      841 gacctcgcac tggctgcgcc accggcgccg ctagaccttc gcctcgagtc ggcgcatcat
      901 caccgtgtgg ccgagctgcc caccggcacc gtcacctttc tcttcaccga cgtcgagggc
      961 tcgacgcggc tgttgcacga gctgggcgac gcctacgccg acgcgctcgg caagcatcgg
     1021 aagacgttgc gtgaatgctt catgcggcac ggcggcgtcg aggtggatac gcagggagac
     1081 gcgttcttcg tcgccttcgc gagggcctcg gacgcacttg ctgcggctgc cgacgggcag
     1141 gctgcgctcg agcccggccc gatccgcgtg cgcattgggc tccacacggg cgagccgctc
     1201 gtcaccgacg agggatacgt cggcatcgac gtccatcgcg cggctcgcat tgcggcggtc
     1261 ggctacggcg gacagatcct cgtctcgcag tcgacgcgcg acctcgcgcg cgcccacggg
     1321 ctacgcgacc ttggtcatca ccggctcaag gacctcaccg cgcccgaacg catctaccag
     1381 ttcggcgacg acgacttccc cccgctgaag agcctcaaca cgacgaacct tccggtcgcg
     1441 tcgaacccgc tcgtcggtcg tgagacagag ctcgccgagc tggcggcgat cctgatggac
     1501 tcggaacggc tcgtcaccct gaccggcccc gggggaagcg gcaagacgcg gttgggcctg
     1561 caggtgggag ctgagctctt ggagcacttt cccggcggcg tgttcttcgt tccgctagcc
     1621 gccctctctg ctccggagct ggtccggcct gcaatctcac gcaccaccgg cgttccccga
     1681 ttggaagagc tggccggccg acgagcgctc ctgctgctcg acaacttcga acatctgctc
     1741 ggcgcggccc ctgacgttgc cgcgctcctc gaagcgggcg actccgcgaa agtgctggcg
     1801 acaagccgcg cgccgcttcg tgtccatggc gagtgcgagt tcgcagtcga cccgttgccc
     1861 gagagcgacg cagtcgagct gctcgtgcag cgcgcccggt ccgtgcggcg agatttcgcc
     1921 cccgacgagc acgcgaagga gatctgtcgg cggctcgacg ggctcccgct tgccgtcgag
     1981 cttgctgctg cgcggctgcg ctcgctcgac tccgcggcac tcctcgatcg gctcgatcgg
     2041 cgtctgccgc tcctgaccgg gggcgcgcgg aacgcacctg agcgccagcg cacgctgcgc
     2101 gcgacgatcg aatggagcta cgacctcttg cccgacgact tgtccgcgct tttcgccagg
     2161 ctggcagtgt tcgcgggcac attctcgctc gacgccgcag agcaggtcgc cggctgcaca
     2221 gtcgagacgc ttgatgcctt ggtggaggcg agtctcgtga aagcgctcgc cggtaatcgg
     2281 ttcctcatgc tggagacgat ccgggagctg gcactcgaac ggtttgccga gctcgatgac
     2341 gtggccgagc tccgcgcgcg gcacgccagg tactaccact cggtcgcgct gagcgcctgc
     2401 ctcgatacgg atgtaccgga cgagcagcgc ccggagatcg tctttcccga ggctccgaac
     2461 cttcgcgtcg ccctcgcctg ggcgctggag accagcgaga ccgatttcgg ccttgacctc
     2521 ctggttgctc tggagcagtt ctgggtcctt ggttacacca ccgaggcgat gcgttggttc
     2581 accgcgttcc tcgagcatgc ggacggagcc ccgccgcttt tgcgtgctcg agcactccgg
     2641 tcattcggca gctccgcaca cttcgccggc gaattcgagt tcgccgagcg cctctgcgag
     2701 gagagcctcg ccgagtacga gcagctcggc gacgaacacg gcattgcggt gctgctgcac
     2761 cgcttgtcga tctttgcgct ccaccggggc gacatcgagc aggcgagtgc gctcagtgaa
     2821 cggagcctcg agcttcatcg actacttgcg aacgataagg gggctgcgca gccgctcgcc
     2881 ctgctcggcg cgatcgccct ccagtcgggt gaccgcgagc gaggacgggc actcctcgag
     2941 gagagcgccg agctcgcagg caagatccgc tggcgctggt ggcgggcagg gacgctcagt
     3001 gcactcgcgg aggtggcgat cgcggacgat cgcatcgccg acgcccaggg acttctgcac
     3061 gaggctgtcg atctggccct tcaattcggt gatcgcgttg ggctctcgtg gtacctcagc
     3121 cagtacgcgc tcacacttct gcatgactcg cggacggagg acgccggacg ggtctggggt
     3181 accgtcgagg cggccgccgc attcgttccg ggtggcccgt ggccgcgtga cctcgatcgg
     3241 cttcagcacg aagtgctggc tttggccgac gccgcgttcg aaacaggtcg cgaggcgggt
     3301 gggtcactct ccctcgagga ggctgcgacc cggatgtgcg ccatagactg acggcgtggc
     3361 cccgcctgca gacctgcgcg cgtggatcgc gctgctagag cgcgagggtg agctcgtccg
     3421 catcgccgcc gaggtcgatc ctgaccgtga gatcacagag atcaccgacc gcacggtcaa
     3481 ggccggcggc cccgcgctcc tgttcgaaaa cgtcaagggc tcctcgcatc cgttgctgat
     3541 caaccagttc ggcaccgagc gtcgaatgtg tatggccttc gacgcgccct cgctcgacag
     3601 cgtcgccacg aagctgagcg atgtcctcga gatgcagcc
//
LOCUS       DASVEU010000022         6755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1185276,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000022 DASVEU010000000
VERSION     DASVEU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185276"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF225_00875"
     CDS             <1..232
                     /locus_tag="VF225_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143835.1"
                     /translation="TTRRSNGSVQVTYKEHPLYVFALDKKAGQTNGQGNVAFGGKWYA
                     VSAKGAAVVKAPTPSTTTTTTTTTTTNPNPYP"
     gene            complement(488..561)
                     /locus_tag="VF225_00880"
     tRNA            complement(488..561)
                     /locus_tag="VF225_00880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(525..527),aa:Ile,seq:cat)
     gene            997..4251
                     /locus_tag="VF225_00885"
     CDS             997..4251
                     /locus_tag="VF225_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7143836.1"
                     /translation="MPICASCGQDNPDVAKFCLACGAPLAAQPTTEERKLITVLFTDI
                     VGSTAKAENMDPEDVRARLAPYYVRLRTELERFGGTVEKFIGDAVVALFGAPVAHEDD
                     PERAVRAAFAVCRAIDELNAEDEWLDLQVRVGVNTGEALVVVGARASEGEGVASGDVM
                     NTAARIQSAAPVNGVLVGELTYTATRDAIDYRDAEPIAAKGKSEPVRVWEAVEVKEEA
                     HMPAADAVTFVGREREASALTEVWSTAIAERRPSLVTIVGPPGIGKSRLLAEFARHAG
                     QEGRVHWGRCLPYGEGITYWPVTDVVKSAAGILQSDDRETIAGRLDDFLKTLPTDDLD
                     ELRTIAAALSNLIGSPTTPHGTYAAAEISQGELHWGIRRMLQLLAAQEPTALILEDLH
                     WAEPTLLDLIAYIVATDDTESRLAIVCSTRPELAETSPGFLGTDGRRRTIELGTLDGE
                     QAAALLTDLLGDPSLVETPFASSLMANAGGNPLFLEETVRMLRDRGLVDAERWRSEEM
                     GELPVPTSVQGLISSRLDRLEGGDKRLAHNASVVGAVFWAGAVAHLGVEDGGPPPDPH
                     VGLETLERRDFVLHSEISTVAGEDEYAFKHILMRDVAYGQVPKGRRAELHVRFSDWVT
                     ILPGSADEFVEIVAWHLEQACRLSREVARSPIEPPLLQAAGALANAARRAERREGLHE
                     AERYYTRALDVLRDEHPGVQLDLRLRRADIMMMLGRLKEACEELVEVAEAASRFDRTD
                     VECEALLLLGDIDQRQGQAAEAHRRLAEAQSLAEYTGNAVLRSKVAFVLAALVADFDG
                     EHDRAIDSLRSGVATAEEIDDTPLVAEGHLRLAAILLNRGEFAEAETELRRCLELARE
                     LGSHRVEAEATSWLGMITYHRGDPEEGERLCRQARQWFERTGDTYFQVQNLVRGLALF
                     ALADDRPEDAETWLREAVPAALQIGGWVVVEAYRFLVEALVAQERLDDARELVAFAAR
                     NLPEEDAGARSSLLLAEASVATATGEATTASTAFAEALRLIEELEMPLELADARMALG
                     LSLRTFGDLTGARAELERARAMFVRIGATTRRDAIDRELVELVEGPAAAGPSTL"
     gene            complement(4377..6311)
                     /locus_tag="VF225_00890"
     CDS             complement(4377..6311)
                     /locus_tag="VF225_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143837.1"
                     /translation="MAIVFENVPKASGLTQTASLAAQITQQATGASNSNFACVYQAVS
                     IDGTTNVSGKKGTPVTVTLNAHQSVAITQDAHGGGNTLQSATLAGACDSGNPLTQSQT
                     LSSTATGSSSITQNENDADSGPNVSLDIKQNQSAGYFGDAATTGLNKAAFSQTNVLTA
                     IANTPGPSVSQTQSSPSGGISANVNQFSHGKSTANATQSETQCAHAQTSGSPTCTAGS
                     PPSYSLTQTQYGPVKNAAGKSATAASRSFAFVRKDPGSTQGDNSQDTFTINQSSTQSN
                     DTGQNQMNEVQGACTTSGNCTSTQTTNENGQQTQTTQSGQNVNSSINCPSGSSCTATA
                     PPTPIIDSGPDASTTSTSATFTFHDADPTATFLCRLDTGGYSPCSTGQSYTGLSAGSH
                     TFYVKATDQYGNASAPATYSWTIASGGSANVLVAGTGDVGSTVPNDNLTQQLTSAGYS
                     VTESASLPADLSTFGQVWWIASNAPTSGEQNQLIDFVKSGRGVFLTGERPCCEALNAA
                     DQSIVNSVVVGGGITVGGQGDVCSCNTPLPVNPDVVGNVANQPFTVTTWQPAAPGGMA
                     NVPASSVFAYYQPGDLSTRQVAAAVWDRSSITGNGRLAVFMDINWTEPTYRATNWSDV
                     AENVAFFLSGLGSPPSPPGP"
     gene            6310..6513
                     /locus_tag="VF225_00895"
     CDS             6310..6513
                     /locus_tag="VF225_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143838.1"
                     /translation="MLSVALFWLIEHDWPNPVVLMQIALFGAAAAVRGLVVFVAVPDL
                     VACTTAQLPVVQKNVFLPPAAAI"
BASE COUNT         1068 a         2245 c         2341 g         1101 t
ORIGIN      
        1 cacgaccagg cgtagcaacg gcagcgtgca ggtcacctac aaagaacacc ccctctacgt
       61 cttcgcgctc gacaagaagg ccggtcagac aaacggccag ggcaacgtcg cctttggcgg
      121 caagtggtac gccgtctctg ccaagggggc ggcggtcgtc aaggctccca ctccttcgac
      181 gaccaccacg accaccacga ccaccacgac gaaccccaac ccgtacccct aaccggtagc
      241 gacccgcgtc ccgcataagc tcggcaccgt gcgcgaccct cacgcagacg tgccgacctg
      301 cgggacgctg tatgtgccga cggtgttcca cccgagcacc cagaaagaag tgccgcgagc
      361 accaagcagg ccactatgcg gctcattgcc cgaatctacc gagagattgc ttgcaaaact
      421 gcttgcaaaa ggggatcgac tcaagccctc ccagccgcag aaatccgcag ttccagggag
      481 ttcggagtgg gcccgcctgg attcgaacca gggaccaacg gattatgagt ccgctgctct
      541 aaccagctga gctacgggcc cgctgcggcc acaccgtatc gcggaaaccg aacggcatcc
      601 ctactccata agaggaagcg atgcgccgcc tgctcgctct cctcccgacc tcgctgctcc
      661 tgggcgctgc ggaggcagct cgagtacatc ggtgggcgga gcacaggccg agcagacgat
      721 ccagatctcc gaggaaggag tacgcgctca ccccgtccac cgttgaagca ccctgttccg
      781 ctttgagcgc ggggagggtt tcggcgagat gtgcgtcacg ctcgcctcga tcgatttgat
      841 ccagaacctt cgtcaccgtc gcggctagac cggcccgatc ggcggcaccg gcgtcggggc
      901 ctgggtcatc aaaaagcgct tctcaggcag cgccggtgtg gctcaaccgg cggcggccgc
      961 cttgcggagc ctggacggca gagcgacact cgcggcatgc ccatttgcgc gagctgcgga
     1021 caggacaacc cggacgtcgc gaaattctgc ctcgcgtgcg gcgcgccgct cgctgcgcag
     1081 ccgacgaccg aggagcggaa gctcatcacg gtcctcttca cggacatcgt cggctcgacc
     1141 gcgaaggccg agaacatgga cccggaggac gtccgcgcgc ggctcgcgcc ctactatgtc
     1201 cgcctccgga ccgagctgga acgcttcggc ggcacggtcg agaaattcat cggcgacgca
     1261 gttgtcgcgc tcttcggcgc gccggtcgcg cacgaggacg atccggagcg agccgttcgc
     1321 gcggcgttcg ccgtctgccg cgcgatcgac gagctgaacg ccgaggacga gtggctcgat
     1381 ttgcaggtcc gcgtcggcgt caacacgggc gaagcgctcg tcgtcgtcgg cgcgcgcgcg
     1441 agtgagggcg agggcgtcgc ctcgggtgac gtgatgaaca ccgccgcgcg catccagtcg
     1501 gcggcgccgg tcaacggcgt ccttgtcggc gagctgacgt acacggccac gcgggacgcg
     1561 atcgactacc gcgacgccga gccgatcgcc gcgaagggca agtcggagcc ggttcgcgtc
     1621 tgggaggcgg tcgaggtgaa ggaggaggcc cacatgcccg cggccgacgc cgtcacgttc
     1681 gtcggccgcg agcgtgaggc gagcgcgctg acggaggtgt ggagcaccgc gatcgccgag
     1741 cgccggccca gcctcgtgac gatcgtcggc ccgccgggaa tcggcaagag ccgcctgctg
     1801 gcggagttcg cgcggcacgc agggcaggag gggcgcgtcc actgggggcg atgcctgccc
     1861 tacggcgagg ggatcacgta ctggccggtg acggacgtcg tcaagtcggc ggcggggatc
     1921 ctccagagcg acgaccgcga gacgatcgcg ggcaggctcg acgacttcct caagacgctt
     1981 ccgaccgacg acctcgacga gctgcggacg atcgcggcgg cactgtcgaa cctgatcggc
     2041 agcccgacga cgccgcacgg cacgtacgcc gccgcggaga tctcgcaggg cgagctgcac
     2101 tgggggatcc gtcgcatgct gcagctgctc gcggcacagg agcccacagc gctgatcctc
     2161 gaggatctcc actgggccga gcccacgctg ctcgacctga tcgcctacat cgtcgcgacc
     2221 gacgacacag aatcgcggct tgcgatcgtc tgctcgaccc ggcccgagct cgccgagacg
     2281 tcgcccgggt tcctcggcac ggacggccgg cggcggacga tcgagctcgg cacgctcgac
     2341 ggcgagcagg cggctgcgct gctcaccgat ctgcttggcg acccctcgct cgtcgagacg
     2401 cccttcgcat cgtcgctgat ggcgaacgca gggggaaatc cgctgttcct cgaggagacg
     2461 gtgcggatgc tgcgcgaccg cggcctcgtc gacgcggagc gttggcgtag cgaggagatg
     2521 ggggagctac ccgtcccgac aagtgtccag ggcctgatca gctcgaggct cgaccggctc
     2581 gaaggaggcg acaagcgcct ggcgcataac gcctccgttg tcggcgcggt cttctgggcc
     2641 ggcgcggtcg cccacctcgg cgtcgaggac ggcgggccgc ctcccgatcc ccacgtgggg
     2701 ctggagacgc tcgagcggcg ggacttcgta ctgcacagcg agatctcgac ggtcgcaggc
     2761 gaggacgagt atgcgttcaa gcacatcctg atgcgggatg tcgcgtacgg acaggtcccg
     2821 aaaggccgac gcgcggagct ccacgtccgc ttctccgact gggtgacgat cctcccagga
     2881 agcgccgacg agttcgtcga gatcgtcgcc tggcacctcg agcaggcgtg ccgcctctct
     2941 cgcgaggtgg cccggagccc gatcgagccg ccgctgctgc aggcggccgg agcgcttgcc
     3001 aacgccgccc gccgcgccga acggagggaa gggctccacg aggcagagcg gtactacacg
     3061 cgggcgctcg acgtgcttcg cgacgagcat cccggagtac agctcgacct ccgcctccgg
     3121 cgcgcagaca tcatgatgat gctcggccgg ctgaaggagg cgtgcgagga gctcgtggag
     3181 gtcgccgagg ccgcgtcgcg cttcgaccgc acggacgtcg agtgcgaggc attgctcctg
     3241 ctcggcgaca tcgaccagcg gcaggggcaa gcggccgagg cgcaccgccg gctcgccgag
     3301 gcgcagagcc tcgcggagta caccggtaac gccgttctcc ggagcaaggt ggccttcgtg
     3361 ctcgcggcgc tcgtcgccga cttcgacggc gagcacgacc gcgcgatcga cagcttgcgc
     3421 tccggcgtcg cgaccgccga ggaaatcgac gacaccccgc tcgtcgccga aggccacctg
     3481 cggctcgcgg cgatcctgct gaaccgcggc gagttcgcgg aggcggagac ggagctccgc
     3541 cgctgcctcg agctcgcccg cgagctcggc agccaccgcg ttgaggctga ggcgacgtcg
     3601 tggctgggga tgatcacgta tcaccgcggc gaccccgagg agggcgagcg attgtgccgg
     3661 caggcccgcc agtggttcga gcgaaccggc gacacgtact tccaggtgca gaacctcgtg
     3721 cgaggtctgg cgctcttcgc gctcgcggac gaccggccgg aagacgcgga gacgtggctg
     3781 cgcgaggcgg tccccgccgc cctccagatc ggcggctggg tcgtggtgga ggcgtatcgg
     3841 ttcctcgtcg aagcgctcgt cgcgcaggag cggctcgacg acgcacgcga actggtcgcg
     3901 ttcgccgcgc gcaacctgcc cgaggaggat gccggcgcgc gctcgtcgct gctgctggcg
     3961 gaggcgagcg tcgccacggc gacgggcgag gcgacgacgg cctcgactgc gttcgcggag
     4021 gcactgcggc tgatcgagga gctggagatg ccgctcgagc tcgccgatgc acggatggcg
     4081 ctcggcctgt cgctgcgcac gttcggcgac ctcaccggcg cgcgcgcgga gctggaacgg
     4141 gcgcgggcca tgttcgtgcg catcggcgcc acgacgcggc gcgacgcgat cgaccgtgag
     4201 ctcgtggagc tcgtggaggg gccggcggcc gccggcccct ccactctcta aagcgaccta
     4261 cctggcgcct gcttgcgccg cccgaggcgg tgcgagcagc aggccgggca ggagccgcct
     4321 gtgatcgcgc ggcgtagggc gcagcccctc cggaacgagg ttggcagggc tctctctcat
     4381 gggcccggcg gactaggcgg actaccaagg cctgacagga agaaggcaac gttctccgct
     4441 acgtcggacc agttcgtagc gcggtatgtg ggctcggtcc agttgatgtc catgaacacc
     4501 gccagacgac cgttccccgt gatgctggat cgatcccaga ctgctgcagc gacctgccgc
     4561 gtcgagagat cgcccggttg gtaataggcg aagacggacg aggccggtac gtttgccata
     4621 ccgcctggtg ccgcaggttg ccacgtcgta acggtgaacg gctggtttgc gacgttgccg
     4681 acgacgtcgg ggttcacagg caacggcgtg ttgcagctac aaacgtcgcc ctggcctcca
     4741 accgtgatgc caccacccac gaccaccgaa ttcactatcg actgatcggc cgcgttgagt
     4801 gcctcacaac agggacgctc gccggtgagg aagacgcctc tcccgctctt cacgaagtcg
     4861 atcagctgat tctgctcgcc tgatgtcggt gcgttggaag cgatccacca cacctggccg
     4921 aacgtgctga gatccgcagg aaggctggcc gactcggtca ccgagtatcc ggcgctcgtg
     4981 agctgctgcg tgaggttgtc gttcggcacg gtcgatccaa cgtcgcccgt ccctgccacc
     5041 aagacgttcg cgctcccacc gctcgcgatt gtccagctgt acgtcgcagg agcgcttgcg
     5101 ttgccgtact ggtctgtcgc cttgacgtag aacgtgtgcg agccggcgct caggcccgtg
     5161 tagctctgcc cagtggagca gggtgagtaa ccgccggtgt cgagccgaca gaggaacgtc
     5221 gccgtcgggt cggcgtcgtg gaacgtgaac gttgcagagg tggacgtcgt cgacgcatcg
     5281 gggccggagt cgatgatcgg cgtcggcggc gcggtcgccg tgcaggacga ccccgagggg
     5341 cagttgatgc tgctgttgac gttctgtcct gattgagtgg tctgcgtctg ctggccgttc
     5401 tcgttcgtcg tctgagtcga ggtgcagttc ccagacgtag tgcaggcgcc ctgcacctcg
     5461 ttcatctggt tctggcccgt gtcgttgctc tgcgtcgagc tctggttgat cgtgaacgtg
     5521 tcctgggagt tgtcgccctg ggtcgagccc gggtctttcc tgacaaatgc gaacgagcgc
     5581 gacgccgcgg ttgccgattt gcccgccgcg tttttcaccg gcccgtactg cgtttgcgtc
     5641 agggaatacg acggtgggct tcccgcggtg caggtcggag acccggaggt ctgagcgtgc
     5701 gcgcactgcg tctcggattg tgttgcgttg gcggtcgact tgccgtggct gaactgattc
     5761 acgttggccg aaataccgcc gctcggcgag ctttgggtct gactcacgct tggtcccggc
     5821 gtgttcgcga tcgcggtcag aacgttggtc tgagagaacg ccgccttgtt cagaccggta
     5881 gttgctgcgt ctccgaagta cccggcgctc tggttctgct tgatgtcgag tgacacgttc
     5941 ggtccgctgt ccgcgtcgtt ctcgttctgc gtgatcgaac tcgaacccgt ggcggtcgaa
     6001 ctgagtgtct gactttgcgt gagcggatta ccggaatcac aggcgcccgc cagcgtcgcg
     6061 ctttgcagcg tgttgccacc gccgtgggcg tcctgagtaa tcgcgaccga ctgatgggca
     6121 ttcagcgtca cggtcacggg tgtgcccttc ttgccggaca cgttcgtcgt gccgtcgatg
     6181 ctgacggcct ggtagacaca ggcgaagttc gagttggaag cgccagtcgc ctgctgggtg
     6241 atctgggccg caagcgaggc cgtttgggtg aggcccgacg ccttggggac attctcgaag
     6301 acgatcgcca tgttgtccgt cgcgctcttc tggttgatcg agcacgactg gcccaatccg
     6361 gtcgtcttga tgcagatcgc cttgttcggt gcagcagcgg ccgttagggg cctcgtcgtc
     6421 ttcgttgcag tcccggacct tgtcgcctgc acgaccgcac agcttccggt ggtgcagaag
     6481 aacgtgttcc tgccgccggc cgccgcgatc tgaacgaccg ggtgtgcggt cgacgcgcat
     6541 gcccaacccg cccccgggca attcgggcct gcgtagtttc gagcgcccct ctggatgacg
     6601 aggccgcgcg gatcgagtcc gatcgcgcgc agataccgga ccacgccggc ccgggtgctc
     6661 aggttcgccg ccttcagcag cgtgccgcgg cgcgatatcg cctcgctgac cttgccagcg
     6721 gtcgccgcgt gggacatcgt cgccggagcg agcgc
//
LOCUS       DASVEU010000023        18499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1380429,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000023 DASVEU010000000
VERSION     DASVEU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 18499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18499
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380429"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..289
                     /locus_tag="VF225_00900"
     CDS             134..289
                     /locus_tag="VF225_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143839.1"
                     /translation="MATELHTEHGIVRVPTGTWHVDPAHSSVEFEVKDQKVPVKLLRH
                     GKKITSR"
     gene            325..996
                     /locus_tag="VF225_00905"
     CDS             325..996
                     /locus_tag="VF225_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX7143840.1"
                     /translation="MIDQTDRVRFRDRAEAGRALARALSHYAGRDDVVVLALPRGGVP
                     VGYEVAKELGAPLDVFLVRKLGVPGHEELAMGAIASGGVLVLDEGVLRWLGISEHQLQ
                     RTLAKELDELRRREAAYRDGRSLPDLKGKTVILVDDGLATGASMQAAARAVRRHEPAR
                     IVIAVPVASRATCDRFRDEVDEVVCAVTPAPFHAVGNWYEDFSQTTDDEVRELLALSA
                     ARDSD"
     gene            complement(1004..1384)
                     /locus_tag="VF225_00910"
     CDS             complement(1004..1384)
                     /locus_tag="VF225_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7143841.1"
                     /translation="MSFAPRLAIAWAINLAALWAANAIWDGVRIHGWQAYLIGSAVLG
                     IANAVLKPLLTILTLPLIIITLGFFYLLINIAMIALAEWIAPNFSIDGFWTYVGTVLV
                     VWAVNWIGDQVLGDLQQRARPRLI"
     gene            1407..1709
                     /locus_tag="VF225_00915"
     CDS             1407..1709
                     /locus_tag="VF225_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143842.1"
                     /translation="MFARLHALQTTQEQHDLGLEIVRDQLLPWLRDSSGFRGLIRLWN
                     PDEAKTLVITLWADEESLLASAAAGNQLGELTAETVGTTRLALENYEVSLFDVTDA"
     gene            1693..3258
                     /locus_tag="VF225_00920"
     CDS             1693..3258
                     /locus_tag="VF225_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7143843.1"
                     /translation="MSQTPDFILGDEFAKVLTETTMSLVCVLDQNGRILFFNDACERV
                     TGFARDEVIGGDARDFVIPPEEAEAFGEVLAYVWATGLSSPQVGHWLTKGGERKLIAW
                     SNRLMPSSDGGAALLVTAGIDLSASTPSVAEAEAFGGDPEARLAQVGRLAQEQRALRR
                     VATLVASEASPERVFTAVSEECARVLEVNASAVFRFEGDDTATIVGRHSRAEGAGDAF
                     TLGMRLQADEDTGIGQVLRTGAPARIHDYTERPGEIADSMRLMKYRSSVSAPIVVAGI
                     IWGAVGIASAEPLPADTEARLGAFCELVSLAVASAQAREDLSASRARLVKAGDEQRRK
                     LERNLHDGAQQRLVSLALTIRLARRQLESNAEAAAASLEGAAKELDLALQELRELARG
                     LHPAALTEQGLGPALAGVAERLPIEVDMSAPAERLPENIEATAYYIVSEALTNVAKHA
                     QATKARVDMTLAEKVLKFEITDDGRGGADPSAGSGILGLRDRAEAVGGTLFVISPPGK
                     GTVVTAQIPLSEA"
     gene            complement(3245..4753)
                     /locus_tag="VF225_00925"
     CDS             complement(3245..4753)
                     /locus_tag="VF225_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7143844.1"
                     /translation="MEQVTRRELLARGGRVALVAGGLSLGRPLLELARAGAVGIFDEL
                     ANGLRGEVVLPGASGYEQARLLFDTRFDAIKPRAVVFCESTTDVQRTVRWARKHAVRI
                     VPRSGGHSYGGYSTTSGVIVDVSRLNGVALDPSHRAVVGAGARLIDVYARLAQHGRTV
                     PAGSCPTVGIAGLALGGGVGFASRKYGLTCDNLVEATVVLADGSAVVANAHAHPDLYW
                     ALRGGGGGNFGIVTRLVFRTYPVGQVATYAIEWPWSDAAKVVQAWQKLAPHAPDGLFS
                     VLNLNSAVGGRARITSAGQLFGSADRLRELVKPLANAGMPIRFTVTSRSYLDAQEMWA
                     GCSGTIAECHLPPQGQLGRSTFKGKSSYANRPLSLSGINAMIRQIEARITTGSGSGII
                     LLDSYGGAINRVKKDATAFVHRDALFSMQYLAYWEPSAAAEPNLRWLRNFYAAMRPYV
                     SPYAYQNYIDPDLRNWGRAYYGTNLRRLVAVKQRYDPKNVFRSSQSIPVKLR"
     gene            complement(4778..5470)
                     /locus_tag="VF225_00930"
     CDS             complement(4778..5470)
                     /locus_tag="VF225_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX7143845.1"
                     /translation="MSDSRLTRRGSLVRLGGFLATAVGAAGLKIETSEGAGPAAVASG
                     AVSCVLTPEQTEGPYYIANEAVRRNITDGRPGVPLQLRAFVVNASTCKPIKSAAVDIW
                     HADAGGVYSGFGSGAGNRTFMRGVQRTNAKGLAQFRTVYPGWYQGRTVHIHVKVHLSG
                     NVVHTGQLYFPDALTDAVYRMQPYSSRPNRDVRNAADSIFRNGGSKSLLSVKKNSAGV
                     YAATIMMGVHRS"
     gene            complement(5463..6230)
                     /locus_tag="VF225_00935"
     CDS             complement(5463..6230)
                     /locus_tag="VF225_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7143846.1"
                     /translation="MSVAVQELPGIRRVEQIMGLPVVVDVRDDDEAGPALDQLFDWLR
                     WVDATFSTFKENSEISRINRGGLHREDAHPDVRQVLDRCDRLRDETDGYFDMRAPDGS
                     IDPAGLVKGWAIDRAAGILDGAGLHNYAVSGGGDMRVLGRAIPDLAWRVGIQHPLERH
                     EVAAVIETTDLAIATSGAYARGDHVWNPHSGGAPRGILSVTIVGPELGTADAYATAAF
                     AMGPERAPHWTARLRGYEAMTILADETVFKTGGFPDE"
     gene            complement(6227..6646)
                     /locus_tag="VF225_00940"
     CDS             complement(6227..6646)
                     /locus_tag="VF225_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143847.1"
                     /translation="MRKSITVLLTVAALALPIADATAATRATTKKIVVSKRFTGSVAS
                     VDRWGSLQVTIVVRKTTTITGTKKKVTRKITAVSVPTYPNHTDRSIYINQTALPILKA
                     EALRAQSANINMVSGATDSSDAFAQSLQSAILKAKHS"
     gene            complement(6659..8047)
                     /locus_tag="VF225_00945"
     CDS             complement(6659..8047)
                     /locus_tag="VF225_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX7143848.1"
                     /translation="MNEAGYALESPRRTTLARPRTGRRLATSTAVWVFVAGNAAVLVW
                     LWVHGGNVSEHLSTGELLTSLARITGLLGAYSALLQVLLLARLPPVERLFGFDRLTVW
                     HRWNGHACLDLILAHVVLSVWGYSLTDKISLPKEVSTMLGGGVYPGMITATVGTALLV
                     AVVVTSVVIVRRRLRYEAWYAVHLLAYAGIALAWFHQIPTGNELVLDRVAADYWRALI
                     LGTLALLVAFRIIGPAVNALRYRLRVAEVVAEGPGIVSLRITGRRLDRLDARPGQFFL
                     WRFLDRKRWWAAHPFSLSEAPDGRSLRITVKALGDFSSRLAEVAPGTPVVAEGPFGVF
                     TAAARRRNKIVLIAGGIGITPIRALMEDMSGDVVVIYRVVRDEDVIFRDELEELARER
                     GVTLFFVVGDHATDDGARLLSPGHLQELVPDIAERQVYVCGPPAMTEFLGRNVRRANV
                     PARFIHTEKFAL"
     gene            8220..8837
                     /locus_tag="VF225_00950"
     CDS             8220..8837
                     /locus_tag="VF225_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143849.1"
                     /translation="MTSPRPTSVRDRRATAARLIGGAAIFAAGMLVAGAFTSKAPAQV
                     CLPVVGCVTTTLPTLPLPGVTSPTVPTLPITTTTTTQSTSTTSSTSTTSSTSTTANSA
                     TTPTVPQGAAAFSPKATVRVRGRGARRVVEIRVNLTKAARLNALLSRKRGSIAKRLFT
                     AKAGPHLFKLRIGRAAKPGLASLSLVYRATTGEAARTTHRLRLPR"
     gene            complement(8839..9609)
                     /locus_tag="VF225_00955"
     CDS             complement(8839..9609)
                     /locus_tag="VF225_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7143850.1"
                     /translation="MCFELDSVPPIPVIRGAAVSHEDLVLEAGDGNSFAAFAATPEEP
                     GDVGVVILPDVRGLYRFYEELALRFAERGIAAVALDYFGRTAGVSKRGDDFEYMEHVE
                     QTTAEGIRADVAAAVEYLRSNGARAIFTVGFCFGGRNSWLAAAGGHALAGAIGFYGRP
                     GEGRDGTLGPAQRASELAAPILALQAGADQNISAEDNAAFDAALTAAGVEHDVVTYDG
                     APHSFFDRSQEQFAAASDDAWGKVLGFIEHHTSRRASV"
     gene            9720..10505
                     /locus_tag="VF225_00960"
     CDS             9720..10505
                     /locus_tag="VF225_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7143851.1"
                     /translation="MNVATLHRLPSPRSLASTPHRGLVEVATIFGLYGFYEVVRGQGN
                     ASLTVAREHTDGIVALERHLHLFGERTVQRAAHWVPTLPTILGIAYIALHFLGTAAFL
                     IWLHRTHPERFPIVRNTLIGATGVALAIYILYPVAPPRLAGLGFVDTVTHNAKVNLSS
                     DLLGGLYNPFAAVPSLHFGYALLVGVTVAVLAKGRVARAIGWSYPAVMLLVIVATGNH
                     FFFDAAGGALAIGFGYVAASWVDSPARRARRWQPDRGSAVATC"
     gene            complement(10429..11034)
                     /locus_tag="VF225_00965"
     CDS             complement(10429..11034)
                     /locus_tag="VF225_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143852.1"
                     /translation="MRVRFCDEFDGGFGWIVDEFMQRCSHALVVDGRVWVIDPVDGEG
                     VEERIRAAGTPAGVVQLLDRHNRSCAELAARLGVRHHVVPDGSLGPFACIDVKDSRSW
                     KEVALWWPDNRVLVCADVLGTAPYFRAGNERLSVHPLLRLRPPRRQLGELHPDVVLCG
                     HGEGVLEDADAALREALSTSRRRIPGQAASALRAWRASRPS"
     gene            complement(11456..12067)
                     /locus_tag="VF225_00970"
     CDS             complement(11456..12067)
                     /locus_tag="VF225_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7143853.1"
                     /translation="MPLIPMVVEQDGRYERSFDIYSRLLRERIVFLGQEVEDGIANVI
                     TAQLLFLEAEDPDQEISLYINSPGGSAYAGMAIYDAMQYVKPDVRTICTGMGMSAAAM
                     ILCGGAAGKRFVLPNAKVMIHQGSAGYRGSPADIQIAAKEIIEMTERMAEIIARHTRH
                     PLEQVLKDIDRDHFMTPEEAVAYGLVDEVIKPADARLATKARR"
     gene            12209..12550
                     /locus_tag="VF225_00975"
     CDS             12209..12550
                     /locus_tag="VF225_00975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7143854.1"
                     /translation="MEVTREIVFPDSPGEVWEALTDPEQLEEWFANDVELDAREGGDG
                     VFRWDDGEERRAKVLVVEPGERLVLDWDDQGEVEFTLEEVEEGTRLLVRESTPEFSTA
                     LELRALALSYV"
     gene            12547..12870
                     /locus_tag="VF225_00980"
     CDS             12547..12870
                     /locus_tag="VF225_00980"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7143855.1"
                     /translation="MNDPTGRVFDALADPNRRYVLEALASRDTVTATELAAELPVTRQ
                     AVAKHLAALNEAGLVESRREGRETLYQLTPAPLGAAMDWMASVGADWDARLAALRKHL
                     RNRRS"
     gene            12880..13824
                     /locus_tag="VF225_00985"
     CDS             12880..13824
                     /locus_tag="VF225_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7143856.1"
                     /translation="MERRQLGGTDIEVSRIILGCGNFGGIGSAPEFFGQGESEDEAFR
                     IMDAAWELGITTFDTADAYGGGQSETTIGKWMASRGVRPEISTKTFNPMAEGQDHGLA
                     RARIVRQIESSLDRLGVDSVDVYLAHDFDPDVPVEETVATFEGLIELKLIKAYGVSNF
                     GAQQLEAVLAIAKPAVLQNSYSLLDRADEGGLVQLCLELGIAYTPFGPLAGGWLTGKY
                     KPDEAPPDGSRMAMRPGPYEHLRRPETFIALDGLAKIATRRGVDSATLSIAWLLAQPG
                     VTAVVVGPRRPKHLEPALRALEHPLSAADAEEVGSLFT"
     gene            13821..14378
                     /locus_tag="VF225_00990"
     CDS             13821..14378
                     /locus_tag="VF225_00990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7143857.1"
                     /translation="MRIGFRPLTTSDLPLLYEWLQRKHVKRWWTDRETYDEVVQHYLP
                     EIEGSRPTDPYLILLEDRPAGFIQTYRVSDYPEYRELVNVGDGVAGVDLFVAEPELTG
                     RGFGSEALRRFADDIVFADPGIHACIADPDAENVASLRAFGKAGFRVVRQFVDPSDHD
                     RLHALLRLERQGESRSTSQSPFTRR"
     gene            complement(14330..14932)
                     /gene="pdxH"
                     /locus_tag="VF225_00995"
     CDS             complement(14330..14932)
                     /gene="pdxH"
                     /locus_tag="VF225_00995"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX7143858.1"
                     /translation="MPELRKSDVDPDPLQQFRRWYTEAEGAVSRVPQAMALATSTAEG
                     SPSVRMVLLKDADERGFVFFTGYVSRKAGELEANPRAALLFHWDQLGRQVRIEGSVER
                     VAGQESDAYFATRPRGAQLAAAASRQSAVLRDRAEIDERFAELDREHAGSDVRRPDHW
                     GGYRLVPEAYEFWQHRDDRMHDRLRYRRVNGDWLVERLSP"
     gene            14986..15300
                     /locus_tag="VF225_01000"
     CDS             14986..15300
                     /locus_tag="VF225_01000"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7143859.1"
                     /translation="MAYAQALQALGDPTRRSVLEELRDGPRAVGEIAARLPVSRPAVS
                     QHLRILKEAGLVTERQNGTRRLYRVDPDGLAEVREYLEGFWEEALANFKAAAEQEERR
                     KK"
     gene            15297..15752
                     /locus_tag="VF225_01005"
     CDS             15297..15752
                     /locus_tag="VF225_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7143860.1"
                     /translation="MTATATETIRKEIFVNAAPETAFRVFTEQTAAWWPLQKYGIFLE
                     DAETVVFEDRDGWRLVEKAKDGRETEWGEVLDYELASRIRFTWHPGRDSADEPTEVEV
                     TFTADRDGTLVVLEHRGWENLTDERRAGREGYDNGWPAVLELYRQAAEG"
     gene            complement(15806..16540)
                     /gene="cofE"
                     /locus_tag="VF225_01010"
     CDS             complement(15806..16540)
                     /gene="cofE"
                     /locus_tag="VF225_01010"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX7143861.1"
                     /translation="MSRELRIIPVTGLPEIEENDDLAELITAKVELQEGDVVVLAQKI
                     VSKSEGRVIPLAGIEPSEWAVEIAGDEEDPRRIEVILREAARVVRVRPPLVIAETTHG
                     FICASAGVDASNAPEREMLVLLPHDPDESAKQIRARIRQLTGREVAVLITDSFGRPWR
                     QGTIDVALGASGLEVMRDLRGTRDGVGYELHSTMIAVADEIASAAELVMGKVDGIPAA
                     IVRGLEVAGDGRARDLVIPEERDLFR"
     gene            complement(16537..17154)
                     /locus_tag="VF225_01015"
     CDS             complement(16537..17154)
                     /locus_tag="VF225_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7143862.1"
                     /translation="MKVAIIGGTGAFGRALAKRLHEIGKDEVFVGSRDAERAHETAAE
                     LGIAGGGRNADVVADADLVVLAVKSNATLETAKELAAAIAATPILCVASDLRFTKEGV
                     LPGRHQGSLAEEVARIVRAPVASGLQSFAAVNLIEGDPGDALICGGEDRAKELSLELA
                     DRLADRAIDVGPLENSRALEGMTAVILNVNKRYKAHAGIRLTGLP"
     gene            complement(17151..18083)
                     /gene="cofD"
                     /locus_tag="VF225_01020"
     CDS             complement(17151..18083)
                     /gene="cofD"
                     /locus_tag="VF225_01020"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX7143863.1"
                     /translation="MKVAVLTGGVGGARFLRGIVDAVDPASVTAIVNVGDDLEVLGLS
                     VSPDLDSVLYALAGLADEERGWGRADETWNALATVESLGGESWFRLGDRDLGLHVVRT
                     QALKEGAPLSAVTARLTAVLSVETTVLPATDEELRTWLDTTNGSFAFQEWFVARAHRD
                     PVDRVRFAGAPDAKPAPGVLDALHEADLILVAPSNPFVSIGPILAVERIRSALERRRV
                     PCVAVSPLIGGLAVKGPAAGMLQRLQGGTTAVHVASCYPGLIDALVIDEKDAADADAV
                     ANTGVRPIVTHTLMRDAGARRRLAEAALDAVAFA"
     gene            complement(18080..>18499)
                     /locus_tag="VF225_01025"
     CDS             complement(18080..>18499)
                     /locus_tag="VF225_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143864.1"
                     /translation="KVAVLTGGVGGARFLRGIVDAVDPASVTAIVNADVPCVVPDDLQ
                     SLLAATPLGGIALVASADGRTNALSLPDPNVFDPLYGPRSAKRFQRAARDLGLEAVAV
                     AIPNLVDDVDTPEDLDRLQLRAGPHTQAALQALTLAS"
BASE COUNT         3128 a         6196 c         6306 g         2869 t
ORIGIN      
        1 tgccgaggga gtcgagcgcc gtccacgacg gcttctggga ttgaccggct agggcacctg
       61 gaccacacct gggacgttcg cggccgccac ccgcggggct acaagacagg aaagccaagg
      121 aggaggtccg accatggcga ccgagctcca caccgagcac gggatcgtac gcgtgcccac
      181 aggcacgtgg catgtggacc cggcgcactc gagcgtcgag ttcgaggtga aggatcaaaa
      241 ggtgcccgtg aagctccttc gacacgggaa gaagatcacg tcacggtgac gctcggcgag
      301 ctacggggtt gagacgtgtc ggacatgatc gatcagaccg atcgagttcg gttccgtgac
      361 cgcgccgagg cggggcgcgc cctcgctcgc gccctctccc attacgccgg gcgtgacgac
      421 gtcgtcgtgc tcgcattgcc gcgcggcggc gttcccgtcg gctacgaggt ggcgaaggag
      481 ctcggagcgc ccctggacgt cttcctcgtc cggaaactgg gcgttcccgg tcacgaggag
      541 ctcgcgatgg gcgcgatcgc gagtggcggc gttctcgtgc tcgacgaggg cgttctgcgc
      601 tggctcggta tcagcgagca ccagctccaa aggaccctcg caaaggagct cgacgagctc
      661 cgacgacggg aagccgctta tcgcgacggc cgctcgctgc ccgacctgaa ggggaagacc
      721 gtgatcctcg tcgacgacgg tctggccacc ggcgcttcaa tgcaggcggc ggcgcgcgca
      781 gtccggcgcc acgagcccgc ccggatcgtg atcgccgttc cagtcgcgtc acgcgcgacg
      841 tgcgacaggt tcagggacga ggtcgacgag gtcgtgtgcg cggtaacgcc cgcgcccttc
      901 cacgcggtcg ggaattggta cgaagacttc tcgcaaacga ccgacgacga agtgcgcgag
      961 ctgctcgcgc tcagcgcggc gcgcgattcg gactagcgct tcgtcagatc agacgcggcc
     1021 gagcgcgttg ttgcagatcg ccgagcacct ggtcgccgat ccagttgacc gcccagacga
     1081 cgaggacggt gccgacgtac gtccagaacc cgtcgatgga gaagttgggg gcgatccatt
     1141 ccgccagcgc gatcatcgcg atgttgatca ggaggtagaa gaagccgagc gtgatgatga
     1201 tcagcgggag cgtcaggatc gtgagcagcg gcttcagcac cgcatttgcg atcccgagga
     1261 cggcggagcc gatcaggtag gcctgccagc cgtggatccg gacaccgtcc cagatcgcgt
     1321 tcgcagccca gagggccgcc aggttgattg cccaggcaat tgccagacgg ggcgcgaagg
     1381 acatggcgac agtgtaaaac gacagcgtgt tcgcgcgctt gcacgccctc cagacgacgc
     1441 aggagcagca cgacctcggc ctcgagatcg tgcgtgacca gctccttccc tggctccggg
     1501 acagctccgg cttccgcggg ctgatccggc tctggaaccc tgacgaggca aagacgctgg
     1561 tgatcacgct gtgggccgac gaggaatcgc tcctggcgag cgcggcggca gggaaccagc
     1621 tcggcgagct cacggcggag acggtgggaa cgaccaggct cgcgctcgag aactacgagg
     1681 tgagcctctt cgatgtcaca gacgcctgac ttcatcctcg gcgacgagtt cgccaaggtt
     1741 ctgacggaga cgacgatgtc gctcgtctgc gtcctcgatc agaacggacg aatcctgttc
     1801 ttcaacgacg cctgcgagcg cgtgacgggc ttcgcccgcg acgaagtgat cggcggcgac
     1861 gcccgcgact tcgtcattcc tccggaggag gcggaggcgt tcggtgaggt gctcgcatac
     1921 gtctgggcca cgggcctttc gagcccccag gtcggtcact ggctgacaaa gggcggggag
     1981 cggaaactga tcgcgtggtc gaaccggttg atgccgagtt ccgacggcgg cgcggcattg
     2041 ctcgtgaccg cgggcatcga tctgagcgcg agcacaccga gcgttgccga agcggaggcg
     2101 ttcgggggcg acccggaagc gaggctcgcg caggtcggcc gcctggcgca ggagcagcgc
     2161 gcgctccggc gtgtcgcgac actcgtcgcg tccgaggcaa gtcccgagcg cgtgttcacg
     2221 gccgtctcgg aggagtgtgc gcgtgtgctc gaggtgaacg catccgcggt cttccgcttc
     2281 gaaggcgacg acacggcgac gatcgtcggc cgtcacagca gagcagaagg agcaggcgat
     2341 gcgttcacgc tcggaatgcg tctccaggcc gacgaggaca ccggcatcgg ccaggtgctg
     2401 agaaccggag cgcccgcgcg gatccacgac tacaccgaac gacccgggga gatcgcggac
     2461 agcatgagac tgatgaagta ccgctcgtcg gtatccgccc cgatcgtcgt tgcgggcatc
     2521 atctggggcg ccgtcgggat cgcgagcgcc gagccgctgc cggccgacac ggaggctcgg
     2581 ctgggtgcgt tctgcgagct cgtgtcgttg gcagttgcga gcgcgcaggc gcgcgaggac
     2641 ctgagcgcct cgcgcgcgcg cctcgtcaag gccggcgacg agcagcgcag gaagctcgag
     2701 cgcaacctcc acgacggcgc gcagcaacgg ctcgtctccc tggcgctgac gatccggctc
     2761 gcccggcgtc agttggagtc gaacgcggag gccgccgcag cgtcgctcga gggtgccgcc
     2821 aaggagctcg acctggcgct ccaggagctg cgagagctgg cgcgcggctt gcatccggcg
     2881 gcactcaccg agcagggcct cgggcctgcg ctggcgggtg ttgcagagcg gctgccgatc
     2941 gaagtcgaca tgagcgcacc ggccgaacgg ctgccggaga acatcgaggc aacggcgtac
     3001 tacatcgtct cggaggcgct caccaacgtc gccaagcacg cacaggcgac aaaagccagg
     3061 gtcgacatga cgctggccga gaaggtattg aagttcgaga tcacggacga cggccgcggc
     3121 ggcgccgatc cttcggcggg ctcgggcatc ctcggcctcc gcgaccgtgc cgaagcggtc
     3181 gggggcacgc tgttcgtgat cagcccgccg ggaaagggca cggtcgtgac ggcgcagatc
     3241 ccgctcagcg aagcttgacg ggaatgctct ggctcgaacg aaagacgttc ttcgggtcgt
     3301 agcgctgctt gacggcgacg aggcggcgga ggttcgtgcc gtagtacgca cgcccccagt
     3361 tgcggaggtc gggatcgatg tagttctggt acgcgtacgg cgaaacgtag ggccgcatcg
     3421 ccgcgtagaa gttccgcagc cagcggaggt tcggctcggc ggcagctgag ggctcccagt
     3481 aggcgagata ctgcatcgag aacagcgcat cccggtggac gaatgcggtc gcatccttct
     3541 tcacgcggtt gatcgcaccg ccgtaggagt cgagcaggat gattcccgag ccgctgcccg
     3601 tggtgatgcg cgcttcgatc tgccggatca tggcgttgat cccgctgagg ctcagcggcc
     3661 tgttcgcgta gctcgacttg cccttgaacg tggagcggcc gagctggccc tgcggcggca
     3721 gatggcactc ggcgatcgtg ccggagcagc ccgcccacat ctcctgcgcg tccaggtagc
     3781 tccgtgatgt cacggtgaag cgaatcggca tgccggcgtt cgcaaggggc ttgacgagct
     3841 cgcgcagccg gtctgcgcta ccgaagagct gcccggccga ggtgatgcgc gcgcggccac
     3901 cgacagctga gttcaggttc aacaccgaga agagcccgtc gggcgcgtgc ggcgcgagct
     3961 tctgccaggc ctgtacgacc ttcgctgcat cggaccacgg ccactcgatg gcgtatgtgg
     4021 ccacctggcc gaccgggtac gtacggaaca cgaggcgcgt gacaattccg aaattgccgc
     4081 caccgccacc gcggagggcc cagtagagat cgggatgcgc atgcgcgttg gccaccacgg
     4141 cactcccgtc cgccaacacg accgtcgcct cgacgaggtt gtcgcacgtc agcccgtact
     4201 tccgcgaggc gaagccgacc ccgccgccga gcgcaagtcc ggcgatacca acggtcgggc
     4261 aggacccggc cggcacggtg cgcccgtgtt gcgcgagcct cgcgtacacg tcgatcagcc
     4321 ttgctcccgc ccccacgacc gcccggtggc tcggatcgag agcgacgccg ttcaggcgag
     4381 acacgtcaac gatcactccg gacgtcgttg agtagccgcc gtagctgtgt ccgccggagc
     4441 gtggaacgat tcgcacggcg tgcttgcgcg cccaccgcac agtgcgctgc acgtcggtgg
     4501 tcgactcgca gaagacgacc gcgcgcggct tgatcgcgtc gaagcgggtg tcgaacagga
     4561 gtcgagcctg ttcgtagccc gaggcgccgg gtaggacgac ctcgccgcgc agcccgttcg
     4621 caagctcgtc gaagatcccg accgcgccgg cccgggcgag ctcgagcaga ggtcggccga
     4681 gcgacaggcc gccggcaacc aacgcgacgc gtccgccccg cgcgagcagc tcccggcgcg
     4741 tgacttgctc cacgcgaccg aatctagcga ctcggtgtta agacctatgg acgcccatca
     4801 tgatcgtcgc ggcgtacacg ccggcgctgt tcttcttcac gctcaggagc gacttgctcc
     4861 ctccattacg gaagatcgag tcggcggcgt tgcggacgtc ccggttcggg cggctgctgt
     4921 agggctgcat gcggtagacc gcatcggtca aggcatccgg aaaatagagc tggccggtgt
     4981 gcacgacgtt gccgctgagg tggaccttca cgtggatgtg caccgtgcgg ccctggtacc
     5041 acccgggata gacggtgcga aactgcgcga gacccttcgc attcgtacgt tggacaccgc
     5101 gcatgaacgt gcggttgccg gcgcccgagc cgaagcctga gtagacacca cccgcgtcgg
     5161 cgtgccagat gtcgactgct gcggacttga tcggcttgca cgtcgaggcg ttgacgacga
     5221 aagcgcggag ctggagcggc acgcccggac ggccgtcggt gatgttgcgg cgcacggctt
     5281 cgttcgcgat gtagtacggg ccctcggtct gctcgggcgt caggacacac gacaccgcgc
     5341 cggaggcgac cgccgcaggc ccggcaccct cggaggtctc gatcttgagc cccgccgcac
     5401 cgactgcggt ggccagaaat ccgccgaggc gcacgaggga gccgcgccgg gtgagccggg
     5461 agtcactcat cgggaaaacc tccggtcttg aagaccgtct cgtcggcgag gatagtcatc
     5521 gcctcgtagc cgcgtagccg cgctgtccag tgcggagcac gttcggggcc catggcgaag
     5581 gcagcggtcg cgtacgcgtc ggcagttccg agctcagggc cgacgatggt caccgacaga
     5641 atgccgcgtg gagcgcctcc agagtgcgga ttccacacat gatctccgcg cgcgtaggcg
     5701 ccggacgtag cgatcgcgag atccgtggtc tcgatcacgg cggcgacctc atggcgttcg
     5761 agcggatgct ggatgccgac gcgccaggcg aggtcgggga tcgcacggcc gagaacccgc
     5821 atgtcgcctc ctccgctgac ggcgtagttg tgtagaccgg caccgtcgag aatccctgcc
     5881 gctcggtcga tcgcccagcc cttcacgagc ccggcagggt cgatcgagcc gtccggagcg
     5941 cgcatgtcga agtacccatc cgtctcgtcc cgcaggcggt cgcagcggtc gaggacctgg
     6001 cgcacgtcgg gatgagcatc ctcgcgatgc agcccgccgc ggttgatccg gctgatctcg
     6061 ctgttttcct tgaaggtgct gaaggtcgcg tcgacccagc gcagccagtc gaacagttgg
     6121 tcgagcgcag ggccggcctc gtcgtcgtca cggacgtcca cgaccacagg gaggcccatg
     6181 atctgctcga ctcggcgaat gcccggcagt tcctggacgg cgacgctcat gagtgcttcg
     6241 ccttcagaat cgcggactgg agcgactgcg cgaacgcgtc actcgagtcg gtggcgccgg
     6301 agaccatgtt gatgttcgcg ctctgcgcgc gcagcgcctc cgctttcaaa atcgggaggg
     6361 cggtctggtt gatgtagatc gagcggtcgg tgtgattcgg ataggtcggg acggagaccg
     6421 cggtgatctt tcgcgtcacc ttcttcttcg tgccggtgat cgtggtcgtc ttgcgaacga
     6481 cgatcgtcac ctgaaggctg ccccagcgat ccaccgaggc gaccgagccg gtgaagcgtt
     6541 tgctgacgac gatcttcttc gtcgtcgccc gagtggccgc ggtcgcgtcg gcgatcggta
     6601 aggcgagtgc ggcgacggtg aggaggacgg tgatggactt gcgcatcggt tcctttcgtt
     6661 agagggcgaa cttctcggta tggatgaaac gagcgggcac gttcgcacgt cggacgttgc
     6721 ggcccaggaa ctccgtcatc gccggcgggc cgcagacgta gacctgccgc tcggcgatgt
     6781 ccggtacgag ctcttgcagg tgacccggag acagcagacg cgcgccgtcg tccgtggcgt
     6841 gatcgccgac gacgaagaag agggtgactc ctcgctcgcg cgcgagctcc tcgagctcgt
     6901 cgcggaagat cacatcttcg tcccggacga cgcggtagat cacgaccacg tctccggaca
     6961 tgtcctccat cagcgcgcga atcggcgtga tcccgatgcc gcctgcgatc aggacgatct
     7021 tgttgcgacg gcgggccgca gccgtgaaga cgccgaacgg cccctccgcg acgacaggcg
     7081 tgcccggagc gacctccgcg agccggctcg agaaatcgcc cagggctttg accgtgatcc
     7141 ggagcgagcg tccgtccggt gcctccgaaa gggagaaggg gtgtgccgcc caccagcgct
     7201 tccggtcgag gaacctccag aggaagaact gaccgggtcg cgcgtcgagc cggtcgaggc
     7261 ggcgtcccgt gattcgcagc gagacgatgc ccgggccctc ggcgacgacc tcggccacgc
     7321 gcaggcggta ccgcaaggca ttcacggccg ggccgatgat ccggaacgcg acgagcagag
     7381 cgagcgtgcc gaggatcaac gcgcgccagt agtcggctgc gactcgatcc agcaccagct
     7441 cgttgccggt cggaatctgg tggaaccacg cgagcgcgat gcccgcgtat gcgagcaggt
     7501 gcacggcgta ccaggcctcg taacgaagcc ggcggcggac gatcacgacc gaagtcacga
     7561 cgacggcgac gagcagagcg gtcccgaccg tcgcggtgat catgcccggg tagacgccgc
     7621 cgccgagcat cgtcgagacc tctttcggca gtgagatctt gtccgtgagc gagtagcccc
     7681 agaccgacag gacgacgtgc gcgaggatga ggtcgaggca cgcatgcccg ttccagcggt
     7741 gccagacagt caggcggtcg aagccgaaga ggcgctcgac cggagggagg cgcgccagca
     7801 gcagaacctg cagcagcgcc gagtaggcgc cgagcagccc ggtgatccgg gcgagactcg
     7861 tcagaagctc gccggtgctg aggtgttcgg agacgttgcc cccgtgcacc cagagccaga
     7921 ccagcaccgc ggcgttcccg gccacgaaca cccacacggc ggtcgacgtc gccagcctcc
     7981 gtcccgtgcg cgggcgggcg agggtcgtcc ggcgtggcga ttccagggcg tagcccgctt
     8041 cgttcatgga ctgcagagaa ccacccgacc ctgagagtgg cctgagaacc gcgtaagagc
     8101 ttcgtaagag gctgaagctg ctcctttcgg actaactctt cagggggagt tgttgcagtt
     8161 gtcatctgcc gatgttgaca accgttggcg tccacttaac gaaagacgag gttctcgcaa
     8221 tgacctctcc ccgtcccact tccgttcgtg acagacgcgc gaccgcggcc cgcctcatcg
     8281 gcggcgcagc gattttcgct gccggcatgc tcgtcgcggg cgcgttcacg agcaaggcac
     8341 ctgctcaagt ctgtcttcct gtcgtcggct gcgtcaccac gaccttgccg acattgcccc
     8401 tgccgggcgt gacctcgccg actgtgccca cgcttcccat cacaacgacg actacgacgc
     8461 agagcacctc cacgacgagc tcgacctcca cgacgagctc gacctccacg acggccaact
     8521 cggcgacgac gccgaccgtg ccgcagggtg ccgcggcctt tagtccgaag gcaaccgtcc
     8581 gtgtgcgcgg acgaggtgcg cggcgcgtcg tcgagatccg ggtcaatctc accaaggcgg
     8641 cgcgcctgaa cgcgctcctc agtcgcaaac gcggctcgat tgccaagcga ctgttcacgg
     8701 ccaaggccgg cccgcatctc ttcaagttgc ggatcgggcg cgcggctaaa ccgggacttg
     8761 cgagcctgtc actcgtctac cgcgccacga caggcgaggc ggctcgcacg acgcatcgac
     8821 tgcggttgcc gcgctgagct agaccgaagc ccgccggctg gtgtggtgct cgatgaaacc
     8881 gagaaccttt ccccacgcat catcagaggc cgccgcgaac tgctcttgac tgcggtcgaa
     8941 gaagctgtgc ggcgcgccgt cgtacgtgac gacgtcatgc tcgactcccg cggccgtgag
     9001 cgccgcgtcg aatgcggcgt tgtcttccgc gctgatgttc tggtcggctc ctgcctggag
     9061 ggccaggatc ggggccgcga gctcggaggc gcgctgagcc ggcccgagcg taccgtcccg
     9121 tccttcgccg gggcgcccgt agaagccgat cgcccccgcg agagcgtgcc cgcccgcggc
     9181 cgcgagccag gaattgcggc cgccgaagca gaagccgacg gtgaagatgg cccgagcgcc
     9241 gttcgagcgg agatactcga cggcggcggc gacatcggcc cgaatgccct cggccgtcgt
     9301 ctgctcgacg tgttccatgt actcgaagtc gtcgccgcgc ttcgacaccc ccgcggtgcg
     9361 accgaagtag tcgagcgcaa cggccgcgat cccgcgttcg gcgaaacgca atgccagttc
     9421 ctcgtagaac cggtagaggc cgcgcacgtc cggcaggatc acgacgccca cgtcgcccgg
     9481 ctcctcagga gtcgccgcga aagcggcgaa gctgttgccg tcgcccgcct cgaggacgag
     9541 atcttcgtgc gagaccgcag cgccgcggat caccgggatg ggcggaacgg aatcgagctc
     9601 gaagcacact gctgcgcgag cctaacccag aagcccccac ggaaagacac cagcgagccg
     9661 caactgcagg tagggctagc cgcactgacc ttcgaagccc tcgggcgtag cttgacgcca
     9721 tgaacgtggc gaccctccac cgtctcccat ccccccggag cctcgcgtcc actcctcacc
     9781 gaggcctggt ggaggtcgcc acgatcttcg ggctctacgg cttctacgag gtcgtccgcg
     9841 gccaggggaa cgcatccctg acggtcgccc gtgagcacac cgacgggatc gtcgcgctcg
     9901 agcggcacct gcacctcttc ggcgagcgca cggttcaacg cgcggcgcac tgggttccaa
     9961 cactgccgac catcctcgga atcgcgtaca tcgcgctgca cttcctcggc acagccgcct
    10021 tcctgatctg gctgcaccgg acacatccgg agcgctttcc catcgtccgg aacacgctga
    10081 tcggcgcaac gggcgtcgcg ctcgcgatct acatcctcta ccccgtcgcg ccgccacgac
    10141 tcgcagggct cggcttcgtg gacaccgtca cgcacaacgc gaaggtgaac ctgagttccg
    10201 acctcctcgg gggtctctac aacccattcg ccgccgtccc gagcctgcac ttcggctacg
    10261 cgctgctcgt cggcgtgacc gtcgccgtgc tcgccaaggg ccgtgtcgca cgcgcgatcg
    10321 gctggagcta tccggccgtg atgctgctcg tcatcgtcgc gaccggcaac cacttcttct
    10381 tcgatgcagc cggcggcgcg ctcgcaatcg gtttcggata tgtggccgct agctgggtcg
    10441 actcgcccgc caggcgcgca aggcgctggc agcctgaccg gggatccgcc gtcgcgacgt
    10501 gctgagcgcc tcgcggagcg cggcgtcggc gtcttccagg acgccttcgc catgaccgca
    10561 gaggacgacg tccgggtgaa gctcccccag ttgccgtcga ggagggcgga gcctcagtaa
    10621 gggatggact gacaaccgct cgttcccggc acggaagtac ggcgccgtgc cgagcacgtc
    10681 ggcgcagacg agcacgcggt tgtcgggcca ccagagcgcg acttccttcc aggaacggct
    10741 gtccttgacg tcgatgcagg cgaatggacc gagcgagccg tccgggacaa cgtggtggcg
    10801 gacgccgagc cgagcggcga gttcggcgca ggagcggttg tgccggtcga gcaactggac
    10861 cacgcccgcg ggggtgccgg ccgcccggat gcgctcctcg acgccctccc cgtcgaccgg
    10921 gtcgatcacc cagacgcggc cgtccacgac gagggcgtgc gagcagcgct gcatgaactc
    10981 gtccacgatc cagccgaagc cgccgtcgaa ctcgtcgcag aagcggactc gcaccccgga
    11041 gagagtacgg gtacgcgata ggtcgtctcg agctgggcgg ccacctccac atccttcgcg
    11101 tagagacgga gcggctccgc ggcagcgcct cgatacccgg cttccgtctc gaccaccaca
    11161 cccgacagcc gcacttcacc gagtacccgg caaagcgtcc tcgactcgtc gcggccctgc
    11221 agataggtga gcgcatcgac cggatcccgc gctgcatgga agccgcacgt gcaggcaagc
    11281 gcgggtgcga cgtgcgcttc cccgcacagg cacgcggcaa cggcggcgcc gcctgggtgc
    11341 cacacggtgt cgtagatgac cgaacccagc cgtaccccgg tattcgtctc gatcacggtc
    11401 caggcgcgcc agcccgcgac gcggctgcga ccggcccgga ttccttggcc gatgttcatc
    11461 ggcgtgcctt tgttgcgagc cgcgcatcgg ccggcttgat cacttcgtcc accagcccat
    11521 acgcaaccgc ctcttccggc gtcatgaagt gatcgcggtc gatgtccttc agcacctgct
    11581 cgagtgggtg tcgcgtatgg cgcgcaatga tctccgccat ccgctcggtc atctcgatga
    11641 tctccttcgc ggcgatctgg atgtctgcgg gcgagccgcg gtagccggcg gagccctggt
    11701 ggatcatcac cttggcgttc ggaagcacga atctctttcc ggccgcgccg ccgcagagga
    11761 tcatcgccgc ggcggacatc cccatgccgg tgcagatcgt gcgcacgtcg ggcttcacgt
    11821 actgcatcgc gtcgtagatc gccatcccgg cgtacgcgga gccgccggga gagttgatgt
    11881 agagcgagat ctcctggtcg gggtcctccg cctcgaggaa cagaagctgc gcggtgatca
    11941 cgttcgcaat gccgtcctcg acctcctggc cgagaaagac gatccgctcg cgcagcaggc
    12001 gcgagtagat gtcgaaggag cgctcgtagc gcccgtcctg ctcgacgacc atcggaatca
    12061 gtggcatcta cgcctcctgg taatgtgcaa cccgtcggtt gcgtctagta tacgcaaccc
    12121 agaggttgcg gattccaagg cgtcgactcg tcggcgccgc agagaaggcc gcgcgggctt
    12181 cgcccgggcg gccgtttagg aggaagcggt ggaagtgaca cgcgagatcg tcttcccgga
    12241 ctcgcccggc gaggtctggg aggccctgac cgacccggag cagctcgagg agtggttcgc
    12301 gaacgacgtc gagctcgatg cccgcgaagg cggggacggc gtcttccggt gggacgacgg
    12361 cgaggagcgc cgtgccaagg tgctcgtcgt ggagccgggc gaacgcctcg tgctcgactg
    12421 ggacgaccag ggcgaggtgg agttcacgct cgaggaggtc gaagagggca cgcggctgct
    12481 cgtgcgcgag tccacgcccg agttctcgac cgcactcgag ctccgggcgc tcgctctgag
    12541 ctacgtgtga acgacccaac cgggcgcgtc ttcgacgcgc tcgccgaccc gaaccgccgc
    12601 tacgtcctcg aggcgcttgc gtcacgcgac acggtgacgg cgaccgagct cgcagcggag
    12661 ctcccggtca cacgacaggc ggtcgcgaag catctcgctg cgctcaacga ggccggactc
    12721 gtcgagagcc gacgcgaggg acgggagacc ctctatcagt tgacgcccgc gccgctgggt
    12781 gcggcgatgg actggatggc aagcgtcggc gccgactggg acgcccgcct cgccgcgttg
    12841 cgcaagcacc tgaggaaccg ccgctcgtag actctcgcga tggaacgccg ccagctcggc
    12901 gggaccgata tcgaggtctc gcggatcatt ctcggctgcg gcaatttcgg cggaatcggc
    12961 tccgcacccg agttcttcgg acagggtgag agcgaggacg aggcgttccg catcatggat
    13021 gcggcctggg agctcggcat caccaccttc gataccgccg acgcgtacgg cggcggtcag
    13081 agtgagacga cgatcggcaa gtggatggcg agccgaggcg tccggccgga gatctcgacc
    13141 aagacgttca accccatggc cgagggtcag gaccatgggc tcgcgcgcgc gcgcatcgtg
    13201 cggcagatcg agtcgagtct cgaccgcctc ggcgtcgatt cagtcgatgt ctatctcgca
    13261 cacgacttcg acccggacgt tccggtcgag gagactgtcg cgacattcga agggctgatc
    13321 gagctcaagc tgatcaaggc ctacggcgtc agcaacttcg gcgcgcagca gctcgaggcg
    13381 gtcctggcga tcgcaaagcc agccgtcctg cagaactcct attcgctgct cgaccgtgcc
    13441 gacgagggag gcctcgtcca actgtgtctc gaacttggaa tcgcgtacac gccgttcgga
    13501 ccactcgccg gcggctggct gaccgggaag tacaagcccg acgaggcccc acccgacgga
    13561 tcgcggatgg cgatgcgtcc cggcccctac gagcatctgc gcaggccgga gaccttcatt
    13621 gcgctcgacg gtctcgccaa gatcgcgacg cggaggggag tcgactcggc gacgctgtcg
    13681 atcgcctggt tgctcgcaca accgggggtc acggccgtcg tggtcgggcc ccgccggccg
    13741 aagcacctgg agccggcgct gcgtgccctc gagcatccat tgtcggcagc ggatgccgaa
    13801 gaggtgggct cactcttcac gtgaggatcg gctttcgccc gctgacgacg agcgatctgc
    13861 cgctcctcta cgagtggttg cagcgcaaac acgtcaagcg ctggtggacc gaccgcgaga
    13921 cctacgacga ggttgtccag cactacttgc ccgagatcga aggaagccgg ccgaccgacc
    13981 cctatctcat tctcttggaa gatcggcctg ccggtttcat ccagacgtac cgagtgtccg
    14041 actatccgga gtaccgcgag ctcgtcaatg tcggggacgg tgtggcgggc gtcgacctct
    14101 ttgtcgcaga gccggagctg acgggccgtg gtttcggctc cgaagcactc cgccgcttcg
    14161 cggacgacat cgtcttcgcg gacccgggga ttcacgcgtg catcgccgat ccggacgcgg
    14221 agaacgtcgc ctcactacga gcgttcggaa aggccggctt tcgcgttgtt cgacagttcg
    14281 tcgacccaag cgaccacgac aggctgcacg cgctgttgcg actcgagcgt cagggcgaga
    14341 gccgctcgac gagccagtcg ccgttcacgc gccggtagcg caggcggtcg tgcatgcggt
    14401 cgtcgcggtg ctgccagaac tcgtaggcct cggggacgag gcgatagccg ccccagtggt
    14461 ccggccgtcg cacgtcgctg cccgcatgct cgcggtcgag ctcggcgaac cgttcgtcga
    14521 tctcggcgcg atctcggagc accgcgcttt gacgtgacgc tgctgctgcg agctgggctc
    14581 cacgcggccg cgtggcgaag taggcgtcag actcctgccc ggcgacgcgc tcgacgctac
    14641 cctcgatccg cacctggcga ccgagctggt cccagtggaa gaggagagcg gcgcgcgggt
    14701 tggcctcgag ctcacctgcc ttgcggctga cgtagccggt gaaaaagacg aagccgcgct
    14761 catcggcgtc cttcagcagc accattcgca ccgagggcga gccctccgcc gtggacgtgg
    14821 caagcgccat cgcctgtggc accctcgaga cggccccttc agcctccgtg taccagcggc
    14881 ggaactgctg aagcgggtcg ggatcgacgt ctgatttccg cagctcgggc accgcggcca
    14941 gcctatttga cagatccgag agtaagtgta gacttacggt aagtcatggc ttacgcacaa
    15001 gcattgcagg cgctgggcga tccgacccgg aggtcggttc tcgaagagct ccgcgacggc
    15061 ccacgggccg tcggagagat agccgctcgg ctgcctgtca gccggcctgc agtgtcgcag
    15121 cacctccgga tcctgaagga ggcgggtctg gtgacggaac gacagaacgg gacgcggcgt
    15181 ctgtaccgcg tcgaccccga cggcctcgcc gaagtgcgcg agtacctcga aggcttctgg
    15241 gaagaggcgc tcgcaaactt caaggcggcg gccgagcaag aggaaaggag gaagaagtga
    15301 cggcgactgc aacagagacg attcgtaagg agatcttcgt caacgctgcg ccggagaccg
    15361 cgttccgcgt cttcaccgag cagaccgcgg cctggtggcc gttgcagaag tacgggatct
    15421 tcctcgagga cgccgagacc gtggtcttcg aggaccgtga cggctggcgt ctcgtcgaga
    15481 aggcgaagga cggtcgcgag accgaatggg gcgaagtgct cgactacgag ttggccagcc
    15541 ggatccgctt cacgtggcat cccgggcgtg actcggccga cgagccgacc gaggtcgagg
    15601 tgaccttcac ggccgacagg gacggcacgc tcgtcgtcct cgagcatcgt gggtgggaga
    15661 acctgaccga cgagcgtcgc gcgggccgcg agggatacga caacggctgg ccggcagtgc
    15721 tcgagctgta ccggcaagcc gccgagggtt agcggaccgt ctcacccccg cgcctgggtt
    15781 acgcgccggg acgaacagca cggtgtcatc gaaagaggtc tcgctcctcg ggaatgacga
    15841 ggtcgcgggc gcgtccatcg cccgcgacct ccaggccgcg aacaatcgcc gccggaatgc
    15901 cgtcgacctt tcccatcacg agctcggccg cgctcgcgat ctcgtcggcg actgcgatca
    15961 tcgtcgagtg cagctcgtag ccgacgccgt cgcgcgtgcc tcgtaggtct cgcatcacct
    16021 cgagacccga agcgccgagg gcgacgtcga tggtgccctg acgccacggg cgtccgaacg
    16081 agtcggtgat caggactgcg acctcgcggc ccgtgagctg acggatgcgc gcgcggatct
    16141 gcttcgcgga ctcatccgga tcgtgcggga gcaggacgag catctcccgc tccggcgcgt
    16201 tcgaagcgtc gacgcccgca gaagcgcaga tgaacccgtg cgtcgtttcg gcgatgacga
    16261 gcggcggccg aacgcgcacg acacgcgccg cctctcggag gatgacctcg atccggcgcg
    16321 gatcctcttc gtccccggcg atctcgaccg cccactccga cggctcgatg cccgcgagcg
    16381 ggatgactcg gccttcggac ttcgacacga tcttctgcgc gaggacgacg acgtcgccct
    16441 cttggagctc gaccttcgcg gtgatgagct cggccaggtc gtcgttttcc tcgatctccg
    16501 gcaggccggt gaccgggatg atacgaagct cccggctcag ggcagtccag tgaggcgaat
    16561 gccggcgtgc gccttgtagc gcttgttcac gttcaggatc acggcggtca tcccttcgag
    16621 cgcgcgtgag ttctcgaggg gcccgacgtc gatggcgcgg tcggccaagc ggtcggcgag
    16681 ctcgagggag agctccttcg cgcgatcttc gccgccgcag atcagcgcgt cgccgggatc
    16741 cccttcgatc aggttcacgg cagcgaacga ctgcagtccc gaagcgaccg gtgcgcgcac
    16801 gatgcgcgca acctcttccg cgagcgagcc ctggtggcgc cccggcagca cgccttcctt
    16861 ggtgaaccgc aggtcgctgg cgacgcagag gatgggggtc gcggcgatcg ccgccgcgag
    16921 ctccttcgcg gtttcgagcg tcgcattcga cttgaccgcc agcacgacga ggtcggcgtc
    16981 cgcgacgacg tccgcgttcc gaccgccacc cgcgatgccg agctccgctg cggtctcatg
    17041 cgctctctcg gcgtcccgcg agccaacgaa gacctcgtcc ttcccgatct cgtggagtct
    17101 cttggcgagt gcacgaccga acgcgccggt gccgccgatg atcgcgacct tcacgcgaat
    17161 gcaaccgcat cgagtgcagc ttcggcgaga cgcctgcgcg cgcctgcgtc acgcatgagc
    17221 gtgtgcgtga cgatcggccg aacacccgta ttcgcgaccg cgtcggcatc ggccgcatcc
    17281 ttctcgtcga tcacgagcgc gtcgatcagc cccgggtaac agctcgccac gtgcacggcc
    17341 gtcgtcccac cttgcagtcg ctgcagcatc cccgcggctg gacctttgac agcgagcccg
    17401 ccgatgagcg ggctgacggc gacacacgga acgcgtcgac gctccaaggc ggacctgatc
    17461 cgttcaacgg ccaggatggg gccgatcgag acgaacggat tgctcggcgc gacgaggatc
    17521 agatccgcct cgtgcagcgc gtcgagcacg ccgggcgcgg gctttgcatc cggcgcacct
    17581 gcgaagcgaa cgcggtcgac cgggtcgcga tgcgcgcgtg cgacgaacca ctcctggaag
    17641 gcgaagctcc cgttcgttgt atcgagccac gtgcgcagct cttcgtccgt cgcagggaga
    17701 acggtggtct ccacactgag gacggcggta agtcgcgctg tcacagctga cagcggcgcg
    17761 ccttccttca gcgcctgcgt gcgtacgacg tgcagcccga gatcccggtc accgagacga
    17821 aaccacgact cgccgccgag cgactcgacg gtcgcgagcg cgttccacgt ctcatcagcc
    17881 ctaccccacc cgcgctcctc gtccgccagg ccggctagcg catagagcac gctgtcgaga
    17941 tcaggcgaaa cggagagtcc cagaacttcc agatcgtcgc ccacgttgac gatcgcggtg
    18001 acggacgcgg gatcgaccgc atcgacgatc ccacgcagaa aacgcgcgcc gccgacgccc
    18061 ccggtgagaa ccgcgacctt cacgaagcga gcgtcagcgc ctggagcgcg gcttgtgtgt
    18121 gcgggcccgc gcggagttgc aaacgatcga gatcctcggg ggtgtcgacg tcgtccacga
    18181 gattcgggat cgcgactgca acggcctcga gcccgaggtc gcgcgccgcg cgctggaagc
    18241 gcttcgcact gcgtggcccg taaagcgggt cgaagacatt cgggtcaggg aggctcagcg
    18301 cgttcgtccg accgtcggcc gatgcgacga gggcgatccc gccgagcggc gtcgccgcga
    18361 gcaacgactg caggtcgtca ggcaccacgc atgggacgtc ggcgttgacg atcgcggtga
    18421 cggacgcggg atcgaccgca tcgacgatcc cacgcagaaa acgcgcgccg ccgacgcccc
    18481 cggtgagaac cgcgacctt
//
LOCUS       DASVEU010000024         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_991950,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000024 DASVEU010000000
VERSION     DASVEU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991950"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1972)
                     /locus_tag="VF225_01030"
     CDS             complement(161..1972)
                     /locus_tag="VF225_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7143865.1"
                     /translation="MPALADTTIRLLGQEPLAGRLPTAEQLRIAAILDRAGYAYLEVS
                     GGGCFDSAVRRGVESPWERIRALKAHVKTPLGLALRGRFLVGSRPVDNDFIRRFIACA
                     AENGIDVFRLHDPLNDVSNLREAAAAVARAGCEFEAGLVYSPGRTGENNPLMEQAREL
                     PNLGAVRVLLHDPTGSLQPHRARELVTGLREVTGLPVGIYCQGSGGNALAAALEAARA
                     GADLIASAVYPIALTLHRVSGEALAEALAGLGLDCDIDVAALWEASDVVDEHIGDEPV
                     TPLAPRVAVRAARHDLPASLVAALDTNLRAQSAGARLDETIAELIRIRAEVGWPPLAS
                     PIGQVLGSQALLNVLSASRYTVVVDELRSLVEGHLGTTPAPIEKSIERIVSLTADPES
                     AEDATAPPLQEVRAQAEGLASSEEELLLLGLFGDNAEPLLRTIRGRSSGDESLGAGGV
                     DQARAERIRELVRIVQETGIGEITIEEAGMRVSVRRTEEREPAAAPTESPLAALEDAE
                     LAPIAPNGIVRVEAPMVGTFYRAAQPGAPPFVEEGQPVGAGQTLCILEAMKLMNEVKA
                     DVEGIVRGIHVENAAPVEFGQLLFELEPVVGRPLDAV"
     gene            2018..2620
                     /locus_tag="VF225_01035"
     CDS             2018..2620
                     /locus_tag="VF225_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143866.1"
                     /translation="MDVHELRPGLWRWTASHPEWNHAEHWGPGVGSVYAELPGSVVVV
                     DPLIPEEEEEGFWSALDRDVERLGFPVHVLLTVHWHERSVATVLDRYKATLWRPEEKG
                     ELPAGVHAEVVKGSDWVEALFFLEPHRALVAGDLLIGKDGGVELPVGWFPKGEQEWAE
                     QQLKPELRERLAKLPVELVIVSHGDPVLENGAEALRRALT"
     gene            complement(2617..3183)
                     /gene="efp"
                     /locus_tag="VF225_01040"
     CDS             complement(2617..3183)
                     /gene="efp"
                     /locus_tag="VF225_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7143867.1"
                     /translation="MAETVNTNQFKNGMHIELEGSFWRIVEFQHVKPGKGGAFVRTKL
                     KGLDTGAVVDKTFRAGEKFARVRTETKDMQYLYDSGDEVVFMDENTYEQTSLPRATVE
                     DALEFMLPSSSVQMLLVNGAPTGVQLPASVELAVSETEPGVKGDTVSNVMKPATLETG
                     AVVQVPLFVNTGDRIKVDPREKRYISRA"
     gene            complement(3197..4234)
                     /locus_tag="VF225_01045"
     CDS             complement(3197..4234)
                     /locus_tag="VF225_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7143868.1"
                     /translation="MNPRIERLRESLEEPLLVTNPTNILYLFGFKSSNAALLVEEDRA
                     RLFTDFRYSESARAVEGVEFEETKRALLADLAGRLSGRIGFEADFVSFAGYETLRGGS
                     IEPVPRRGLVERLRAVKDDDELGAIKRACEITDRVFERLVEERFVGRSERDLSWTIEQ
                     LFHDEGAETVAFETIVASGPNSARPHGRATDRQISRGETVIVDTGCVVGGYASDYTRT
                     FTTGFVEGPIKEAYALVLAAQQAGFDALRAGVRGIDADAAARRVVDGTAFAGMFGHGL
                     GHGLGLEVHEAPRLSTESTDTLAAGNVVTVEPGIYLEGRAGIRIEDNVVVTDDGVRNL
                     TGFRKDLITVG"
     gene            complement(4231..4671)
                     /locus_tag="VF225_01050"
     CDS             complement(4231..4671)
                     /locus_tag="VF225_01050"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX7143869.1"
                     /translation="MQVVVLNGVNLDVLARRDPTLYGGMSLSELETRIYEWASTLGCH
                     VRCRQTNHEGEYVEFCHDALDWADGVVVNPGAWTHYSYAIRDALELFDAPIVEVHLSN
                     IDEREVWRRTSVVADLAAQRILGKGADGYREALEFIVQQVGAAQ"
     gene            complement(4732..>5715)
                     /locus_tag="VF225_01055"
     CDS             complement(4732..>5715)
                     /locus_tag="VF225_01055"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX7143870.1"
                     /translation="QPLYEAVSARLVESTEDVLLAALEIDVGRGLLATLSDRLGADPE
                     AVAVIGDEYVLDIHPLDFGPAAVHRVPRGEAAKTPAVLDRLWNELGLDRRGTIVGYGG
                     GSTTDIAGLTAATYLRGVRWIAVPTTLLGQVDAAIGGKTAIDLRAGKNLVGAFHYPSR
                     VIADPGVLATLNEAQRREGMAEVVKTGLLAGRPLWELEDEAMIRACAAFKAAVCLGDP
                     FDRGRRAILNLGHTFAHALEAAAGYEEVTHGDAVALGLLAALRLSGRPTDVVEDVLNP
                     HPVRVDRDRAWAALGRDKKRGLVLLGDDGAHWDVPVPAEDVRRALGELISP"
BASE COUNT          908 a         1975 c         1887 g          945 t
ORIGIN      
        1 cggctttgcc ggtgcggccg tcctggttta gatctagaca gcatccagcg gccttcccac
       61 caccggttcc aattcgaaca gtccgaggca tcgcttgcga tgccgaccgt agaggccgtg
      121 ccggctttgc cggtgcggcc gtctgggcgt agatgtagac ctagaccgca tccagcggcc
      181 ttcccacgac cggttccaat tcgaagagca gttggccgaa ttccaccggc gcagcgttct
      241 cgacatggat tccgcgcacg atgccctcga cgtcggcctt cacttcgttc atgagcttca
      301 tcgcctcgag gatgcagagc gtctgccccg cgccgacggg ctgaccctcc tcgacgaaag
      361 gcggcgcgcc cggctgcgct gcccggtaga acgtcccgac catcggcgcc tcgacacgca
      421 cgatgccgtt cggcgcaatc ggagccagtt cggcgtcctc cagagccgcg agcggagact
      481 ctgtcggcgc agctgccggc tcgcgctcct cggtgcggcg cacggagacg cgcataccgg
      541 cctcctcgat cgtgatctcg ccgatccccg tctcctgcac gatgcgcacg agctcgcgga
      601 tcctctcggc ccgggcctga tcgacgccgc cggcgccgag cgactcgtcg cccgacgagc
      661 gtccgcggat cgtgcggagc agcggttccg cgttgtcgcc gaagagcccg aggagcagca
      721 actcctcctc gctggaggcg aggccttcgg cctgagctcg aacctcctgc agcgggggcg
      781 cggttgcatc ctccgccgac tccggatcgg cggtgaggga aacgatgcgc tcgatcgatt
      841 tctcgatcgg ggccggcgtt gtgccgagat gaccttcgac gagcgagcgc agctcgtcca
      901 cgacaactgt gtagcggctc gcggagagta cgttcagtaa cgcctgcgag ccgagcacct
      961 ggccgatcgg cgaagccagc ggcggccagc cgacctcggc gcggatccgg atcagctcgg
     1021 cgatcgtctc gtcgagtctc gcgcccgccg actgtgcccg caggttggtg tcgagcgccg
     1081 cgacgagact ggccgggagg tcgtgccgcg cggcgcgcac cgcgacacgg ggggcaagcg
     1141 gcgtcaccgg ttcgtcgccg atgtgctcgt caacgacgtc tgaggcctcc cagagcgctg
     1201 ccacgtcgat gtcgcagtcg agcccgagcc ctgccagcgc ctccgcgagc gcctcgcccg
     1261 acacccggtg aagggtgagc gcgatcggat aaacggcgga tgcgatcagg tctgctcccg
     1321 cgcgtgcagc ctcgagcgcg gcggcaagcg cgttgccccc cgagccctgg cagtagatgc
     1381 cgaccggcag accggtgacc tcacgcagcc ccgtgaccag ctcgcgtgcg cgatgtggct
     1441 gcagggagcc ggtcgggtcg tgcagcaaca cgcgcactgc gccgagattc ggcagctctc
     1501 tggcctgctc catgagggga ttgttctcgc cggtccggcc cgggctgtag acgagcccgg
     1561 cctcgaactc gcaccctgcc ctggcgacag cagccgcagc ctcgcgcagg ttggacacgt
     1621 cgttgagcgg gtcgtgcaga cggaagacgt cgatgccgtt ctccgccgca caggcgatga
     1681 atctccggat gaagtcgttg tcgactgggc gagagccaac gaggaagcga ccccggagcg
     1741 caagccctaa cggtgtcttc acgtgcgcct tcagcgcacg gatccgctcc cacgggctct
     1801 ccacgccccg ccggacggcg ctgtcgaaac acccaccgcc cgagacctcg aggtaggcgt
     1861 agccggcccg gtcgagtatc gccgcgatcc gtagctgctc ggccgtcggc aatctcccag
     1921 cgagcggctc ctggccgagc aggcggatcg tggtgtcggc gagcgcgggc atgcggggag
     1981 cctagcgccc tgccgagcca ggataggttt tgcagccatg gacgtccatg agctcaggcc
     2041 cgggctgtgg cgttggaccg cgtcgcatcc cgagtggaat cacgccgagc actgggggcc
     2101 cggggtcggt tccgtttatg cggagcttcc cggttccgtg gtcgtcgtcg atccgctgat
     2161 cccggaagag gaggaggaag ggttttggtc ggcgctggac cgcgacgtcg agcggctcgg
     2221 ttttccggtg cacgtgctgc tgacggtgca ctggcacgag cgcagcgtcg cgacggtgct
     2281 cgaccgctac aaggcgacgc tgtggcgtcc ggaggagaag ggcgagctgc ccgccggcgt
     2341 gcacgcagag gtcgtcaaag gcagcgattg ggtcgaggcg ctgttcttcc tcgaaccgca
     2401 tcgcgctctc gtcgccggcg atctcctcat cgggaaggac ggcggggtcg agctgccggt
     2461 gggctggttc ccgaagggcg aacaggagtg ggccgagcag cagctcaagc ccgagttgcg
     2521 cgaacgcctg gccaagctgc cggtcgagct cgtcatcgtt tcgcacggcg atccggttct
     2581 ggagaacggc gccgaggcgt tacgccgggc gttgacctag gcccgagaga tataccgctt
     2641 ttcgcgcgga tcgaccttga tgcggtcgcc tgtgttcacg aagagcggaa cctgcacgac
     2701 ggcgcccgtc tcgagcgttg cgggcttcat gacgttcgag accgtgtcgc ccttcacccc
     2761 gggctccgtt tcggacaccg ccagctcgac tgacgcgggc agctgaaccc cggtcggcgc
     2821 gccgttgacg agcagcatct gcactgagct ggacgggagc atgaactcga gcgcatcctc
     2881 gacggtcgcg cgaggaagcg acgtctgctc gtaggtgttc tcgtccatga acacgacctc
     2941 gtcgccggag tcgtagaggt attgcatgtc cttcgtctcg gtccgcaccc gggcgaactt
     3001 ctcgccggcc cggaacgtct tgtcgaccac tgcacctgta tccaggcctt tgagtttcgt
     3061 gcggacgaac gctccgcctt ttcccggctt gacgtgctgg aactccacga tgcgccagaa
     3121 cgagccttcg agctcgatgt gcatgccgtt cttgaactgg ttcgtgttga cggtctcggc
     3181 cacgccggga gagtagctag ccgacggtga tcaggtcctt gcggaatccg gtgaggttcc
     3241 gaacgccgtc gtcggtgacg acgacattgt cctcgatcct gatcccggcc cgcccctcca
     3301 gatagatccc gggctcgacg gtcaccacgt tgccggcggc cagggtgtcc gtcgactcgg
     3361 tggagagcct cggcgcttcg tgcacctcca ggcccagccc gtgcccgagc ccgtggccga
     3421 acatgccggc gaaggcggtc ccgtccacca ctcgccgggc ggcggcgtcc gcatcgatgc
     3481 cccgcactcc cgcgcgcagc gcgtcgaagc ccgcctgctg cgccgcgagc acgagggcgt
     3541 aagcctcctt gatcggccct tcgacgaacc cggtcgtgaa ggtccgggtg tagtcggacg
     3601 catagccgcc gacaacgcag cccgtgtcca cgatcacggt ctcgccgcgg ctgatctggc
     3661 gatcggttgc acgaccatgt ggacgcgccg agttcgggcc cgatgcgacg atcgtctcga
     3721 acgcaacggt ttcggcgccc tcgtcatgaa agagctgctc gatcgtccat gacaggtcgc
     3781 gctcgctgcg gccgacgaag cgttcctcga caagccgctc gaaaacgcgg tcggtgatct
     3841 cacaggcgcg cttgatcgcg ccgagctcgt cgtcgtcctt gactgcgcgc agccgctcga
     3901 cgagaccccg tcgggggacg ggctcgatgg agccgccgcg gagcgtctcg tagccggcga
     3961 acgagacgaa atcggcctcg aagccgatgc gccccgacag cctgccggcg agatcggcga
     4021 gcagcgcccg cttggtctcc tcgaactcca caccctcgac cgcacgcgcc gactccgaat
     4081 accgaaaatc ggtgaagagg cgagcgcgat cctcctcgac caggagcgcg gcgttcgagc
     4141 tcttgaaccc gaagaggtaa aggatgttcg tcgggttggt gacgagcagc ggctcctcga
     4201 gggattctcg gaggcgctcg atgcgaggat tcattgcgca gctcccacct gctggacgat
     4261 gaactcgagc gcttcgcgat agccgtctgc gcccttgccc aggatccgct gcgccgccag
     4321 atcggccacg accgacgtgc gccgccacac ctcgcgctcg tcgatgttcg agaggtgcac
     4381 ttcgacgatg ggtgcgtcga acagctccag cgcgtcgcga atcgcatagg agtagtgcgt
     4441 ccaggcgccg ggattcacga ccaccccgtc ggcccagtcc agcgcgtcgt ggcaaaactc
     4501 gacgtactcg ccttcgtgat tcgtctgccg gcaccgaacg tgacacccga gggtcgaggc
     4561 ccactcgtag atgcgagtct cgagctccga cagcgacatg ccgccgtaca gcgtgggatc
     4621 gcggcgagcg aggacgtcga gattgacgcc gttgaggacg accacctgca ccgcgcgact
     4681 ttactctgcg agcgcatctt ctcggctccc aaactcgccg gcgaccgatt gttacgggga
     4741 gatcaactcg ccaagcgcgc ggcggacgtc ctccgccggg acagggacgt cccaatgcgc
     4801 accgtcgtcg ccgagcagca ccaggcctcg cttcttgtca cggccgagcg cggcccaggc
     4861 acggtcgcgg tcgacccgaa ccgggtgtgg attcaacaca tcttcgacca cgtccgttgg
     4921 gcggcccgag agcctcagcg ccgccagcag cccgagtgcg acggcgtccc cgtgcgtcac
     4981 ttcctcgtag ccggctgccg cctccagggc gtgtgcaaag gtgtgcccga ggttgaggat
     5041 cgcgcgccgg cctcgatcga aaggatcacc caggcacacc gccgccttga aggcggcgca
     5101 ggcccggatc atcgcctcgt cttcgagctc ccagagcgga cggcccgcca gcaatcccgt
     5161 cttgaccact tccgccatgc cctcacgtct ttgcgcctca ttcagcgtcg ccagcactcc
     5221 cgggtcagcg atcactcgac tcggatagtg gaatgcaccg acgaggtttt tcccggcccg
     5281 aaggtcgatc gccgttttgc ctccgatcgc tgcatcgacc tggcccaaca acgtcgttgg
     5341 caccgcgatc cagcggacac cgcgcaaata cgttgcggcg gtcagcccgg cgatgtccgt
     5401 cgtcgatccg ccgccgtaac cgacgatcgt gccgcgccgg tcaagcccaa gctcgttcca
     5461 caaccggtcg agaacggcgg gcgtcttcgc cgcttctccg cgcggcacgc gatggacggc
     5521 tgcaggaccg aagtccaatg gatggatgtc gagcacatac tcgtcaccga tcaccgccac
     5581 ggcctcgggg tctgcgccga gccggtccga gagcgttgcg agcagtcctc gcccgacgtc
     5641 gatctccaaa gccgcgagca ggacgtcttc ggtgctctcg acgaggcgcg ccgagaccgc
     5701 ttcgtacagc ggttg
//
LOCUS       DASVEU010000025         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_215375,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000025 DASVEU010000000
VERSION     DASVEU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215375"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VF225_01060"
     CDS             complement(<1..1333)
                     /locus_tag="VF225_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HEX7143871.1"
                     /translation="MPVEFLSDGEAAAYGRFGGQPSRVELERVFFLDDADRALIGRHR
                     GEHNRLGFALQLTTVRSLGLFLADPLEVPGVVLDYLASQLGIADASCVARYVERRTTR
                     FEHAEEIKRTGGLRDFAEVARELEVWVDARAWTTGDGPKAIFNDAIGWLVERGVLLPG
                     VTTLARLVARVRDEATQRLWDTLAGLLTATQCRQLERLLTVPAGRFSDLERWRRGPAK
                     PSGRGLERAIARVAEIKALGLAGLGLDGVVAQRRLVELARYGMSANARTLRRHPRSRR
                     LATLLATVVALEAKATDDCLELLDLLMVTELLGKAERESVREKARQHPRLARASAKLA
                     TAVEVLLDATAPGGGDVGLDELWELIDAVVSRGELRAAVAAITELVPPDSDDDAVKRA
                     QLATRIATVTGFLKTLTGVIEFGATADARAVLAEMQRMPELLRSRKLTSADV"
     gene            complement(1576..1995)
                     /locus_tag="VF225_01065"
     CDS             complement(1576..1995)
                     /locus_tag="VF225_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7143872.1"
                     /translation="MTGHWLGTEVPLTTQHSRPKGMGLHICAVVRQGTSSSSEYVEVV
                     NDGAEPVAVTGLELTDGSDHVYWFPEGDLGPGVTAYVYTESGKNRRMPTGDMILHAGL
                     PASVWKDDSHVAHLRHPDGRIIDTMHAGRPARHPYGH"
     gene            complement(2449..>3417)
                     /locus_tag="VF225_01070"
     CDS             complement(2449..>3417)
                     /locus_tag="VF225_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143873.1"
                     /translation="PSRCTFKVIPLSSDLRLRQPEETLLSRTFRRPDLGRGAHLAARS
                     GLNEPVENVQRAITYLALGPRKRFLPPPSGRFRDALPTRFARRWSYTRRPFIRIDTSS
                     GEDLVWGRRHPLMALRLIIGQLLSGRFQDLAEGSSLRAELGRLAHETGHQFEHDIAEI
                     FRNAELQARENVTMLGGSSIRRVDDRDLGDIDVLVAQASVRALWIVECKDLTGALTPG
                     EVVDEMTEHFGESETASVARVEARRAWVDERRQAALREFGITESPDRWRTKSIIVTGD
                     PVIAPLIRDLPVAVVSATELPRWIGQREARAKTNGRRRRRGNRAKR"
BASE COUNT          540 a         1266 c         1090 g          521 t
ORIGIN      
        1 cgacgtcagc gctcgtcagc ttgcggctgc gcagcagctc gggcatgcgc tgcatctcgg
       61 ccaggaccgc tcgcgcgtcg gcggttgcgc cgaactcgat cacgccggtc agcgtcttca
      121 agaacccggt cacggtcgcg atccgcgtcg cgagctgcgc ccgcttgaca gcgtcgtcgt
      181 cgctgtcggg cgggacgagc tcggtgatcg ccgcgaccgc cgcgcgcagc tcgccccgcg
      241 agacgaccgc gtcgatcagc tcccacagct cgtcaagccc gacatccccg ccccccggag
      301 cggtggcgtc caagagcacc tcgaccgcag tcgcgagctt cgccgacgcg cgcgccagac
      361 gcggatgctg gcgcgccttc tccctgaccg actcgcgctc ggccttgccc aacagctcgg
      421 tcaccatcaa cagatccagc agctcaaggc agtcatcggt cgccttggcc tccaacgcca
      481 cgaccgtcgc cagcagcgtc gccaagcgcc gcgaccgcgg atggcgacgc agcgtccgcg
      541 cgttcgcgct catcccgtaa cgcgcgagct caaccagacg ccgctgcgcc accacaccat
      601 ccagaccaag gccggcgagg ccgagcgcct tgatctccgc gacccgcgcg atcgcccgct
      661 ccagaccgcg gcccgacggc ttcgccggcc cccggcgcca gcgctcaaga tccgagaacc
      721 gcccggccgg gaccgtcagc agacgctcga gctgccggca ctgcgtggct gtgagcaggc
      781 cggcaagcgt gtcccacagg cgttgggtcg cctcgtcccg cacccgcgcg accagccgcg
      841 cgagcgtcgt gacgcccggc agcaacacgc cgcgttcaac cagccaccca atcgcatcgt
      901 tgaagatcgc cttcgggccg tcgcccgtcg tccacgcccg cgcgtcaacc cagacctcga
      961 gctcccgcgc gacctcggcg aaatcccgca gcccacctgt ccgcttgatc tcctcagcat
     1021 gctcaaagcg cgtcgttcga cgctcgacat agcgtgctac gcacgacgcg tccgcgatcc
     1081 ccagctgact tgcgaggtag tccagcacga cgcccggcac ctcgagcgga tcggcgagaa
     1141 acaagcccag cgaccggacc gtcgtgagct gcagcgcaaa cccgaggcgg ttgtgctccc
     1201 cccgatgccg cccgatcaac gcccggtccg cgtcatcgag aaagaacacg cgctccaact
     1261 ccacccgcga cggctgccca ccaaaccgcc catacgcggc cgcctccccg tcgctcagaa
     1321 actcaaccgg caccccgcga cacggtacgc acgatcagcc agcagtagcc cgtcggccct
     1381 tatcgtgact tttcgttccg atgctcctca agggccgact catgcgccgg caggcgccga
     1441 tcttgcgata cacggggtgg aacgacgtta tcggtccggt gcgagcgatc cggcggcgtc
     1501 cgggatcgtg agggaccctt ccgggtagtt gcgcgggctg ctggtcgtgt ccgaggactg
     1561 aggggcggcg gcccctcagt ggccgtaggg atggcgtgcc gggcgcccgg cgtgcatcgt
     1621 gtcgatgatc ctgccgtccg ggtgacgcag gtgggcgaca tgcgagtcgt ccttccagac
     1681 cgacgccggc agtccggcgt gcaggatcat gtccccggtg ggcatccgcc ggttcttgcc
     1741 gctctcggtg tagacgtagg cggtcacacc cgggccgagg tcgccctccg ggaaccagta
     1801 gacgtgatcg gagccgtcgg tcagctcgag gcccgtcacg gccaccggct cagcgccgtc
     1861 gttgacgacc tcgacgtact cgctggacga actcgtcccc tgccgcacga ccgcgcagat
     1921 atgcaggccc attcccttcg ggcgcgagtg ctgggtcgtc agcgggacct cggtgccgag
     1981 ccaatgtccg gtcacgcaca cagcgtaacg agcgagcgcc gcgcgggctc gcccgacgtt
     2041 cgcgccacgc gcgcaggccg tgcgcccgcg ccgggtcggg tgggggagag gaccgtcccg
     2101 caagggaagc ctcagcggca cagcaatcgc cgcgcaggcc gggctacaga gtccagtagc
     2161 tacgggaccc cacggtgtcc cggatgccta gttcgatgac ctgtcgcggt ggacgggggc
     2221 tatacggcac tcggcgcgtg cccgacctcc acatcgatga cgtgcacgtc ggaccggcag
     2281 gcctggactc gccgtcgtcc actcccacca acgaccggat cagtgccgct ctcaccgcat
     2341 gttgcccgcg acgaatgtcg ttggcgatgt cgtccagtat cgagtcgtct tcgctccgtg
     2401 ctcgtccgaa catggcctgt ccaaccgggg cttcgcccgg ccgggccgct agcgcttcgc
     2461 tcggttgccc cgtcgtctcc gtcgcccgtt cgtcttcgct cgggcttcgc gctggccaat
     2521 ccagcgcggg agctccgttg cactcacgac cgccaccggc aagtcgcgga tcagcggcgc
     2581 tatcacaggg tcgccggtca cgatgatcga cttcgtgcgc cagcggtcgg gcgactccgt
     2641 gatcccgaac tcgcgcagcg ccgcttgacg ccgctcgtcc acccacgcgc ggcgggcctc
     2701 gacgcgtgcg accgaagccg tttcagactc gccgaaatgc tcggtcatct cgtcgacgac
     2761 ctcgcccggc gtcagcgccc cagtcaagtc cttacattcg acgatccaga gagcccgtac
     2821 gctggcttgg gcgacgagga cgtcgatgtc gccgaggtcg cggtcgtcga ctcgcctgat
     2881 cgagctccca ccaagcatcg ttacgttttc gcgcgcctgt agctcagcat tgcgaaagat
     2941 ctctgcaatg tcgtgctcga actggtgtcc cgtctcatgt gccagcctcc ccagctccgc
     3001 ccgcagagaa ctgccttctg cgagatcctg gaagcgtccc gacaggagct gcccgatgat
     3061 cagccgcaac gccatcaatg ggtggcggcg tccccacaca aggtcctcgc cgctgctcgt
     3121 gtcgatacgg atgaacgggc gacgcgtgta ggaccagcgg cgagcaaagc gcgtcggcag
     3181 cgcgtcgcgg aagcgccccg acggcggcgg cagaaaccgc ttgcgcggcc cgagcgcgag
     3241 atacgtgatg gcgcgctgaa cgttctcaac cggctcgttg agcccggatc ttgcagcaag
     3301 gtgggcgccc cgaccgaggt cggggcgccg gaacgtccgg ctgagcaggg tttcttctgg
     3361 ttgtcgaagc cgaaggtccg aactcagggg gatcaccttg aaggtgcagc gcgatgg
//
LOCUS       DASVEU010000026         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1575254,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000026 DASVEU010000000
VERSION     DASVEU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575254"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..546
                     /locus_tag="VF225_01075"
     CDS             256..546
                     /locus_tag="VF225_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143874.1"
                     /translation="MPVVLIHQGPTVTAENYRATVQKLAGKDKMESASDWPVGGILSH
                     SAGESPSGFRVVDVWESQEACDAFGQHLGPILEEVGITDPPEMYPAETFVSS"
     gene            complement(808..1176)
                     /locus_tag="VF225_01080"
     CDS             complement(808..1176)
                     /locus_tag="VF225_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7143875.1"
                     /translation="MSEPGPDVMARFVGAENVERGRDYLEQLDPEIHRYIIDFVYGEV
                     YAGEVLDPKTRALCTVAMLASLDQQLQLGVYVQAARRQGASEEEIREVLRQVAVYAGF
                     PNAWNALATMKAALERFEAP"
     gene            complement(1173..1652)
                     /locus_tag="VF225_01085"
     CDS             complement(1173..1652)
                     /locus_tag="VF225_01085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7143876.1"
                     /translation="MSYEFVDQMMEQTKAGKLRSPVSETLDMQLVEFGRGEAIYEMAA
                     REELGNPLGVIQGGVATALADAAMAAATTSILTDEEVQKSAITTIDIFARFIRPVNAK
                     KIERLRAEAKVIRAGGRLVWAEADVIADGEVVGKFASTGIRVAFDPQSYIAGQGASR"
     gene            1688..2419
                     /locus_tag="VF225_01090"
     CDS             1688..2419
                     /locus_tag="VF225_01090"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7143877.1"
                     /translation="MTVRDRSPKRHRTGSRGVPRAVREPLILDVAGRVFASRGYHAAS
                     MDEIAAEADVTKPLIYAYFGSKEGLYIAYIERSGRELLERMRTATQPTAPPRERLQTG
                     ILEFFRFVGERRDGWQVLYSEAASRGGPLAEEVATLRAAIARMITRLLMETIPDLRAS
                     DETTAALDAVAHAIVGAGESLANRSLAHPELPPERAAQLLVAIGQTGVEQTVAALDSR
                     PAGSASLPSSPSMSASSATTAGSGR"
     gene            2780..>3357
                     /locus_tag="VF225_01095"
     CDS             2780..>3357
                     /locus_tag="VF225_01095"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX7143878.1"
                     /translation="MNMPPTVSPQEWKAAREELLVEEKELTHARDALAAKRRRMPRMA
                     VENDYEFEGPNGPASLLDLFEGRRQLIVYRFFFEPGVHGWPDGGCPGCSWIADQITHP
                     AHLNARGTTIALISRAPQTDIGRWKARMGWEILPWYTLTDDFDTDFDVGEWHGTNVFY
                     RDDDDRIFRTYFVDGRGDEALGGTWAYLDITAL"
BASE COUNT          573 a         1168 c         1078 g          538 t
ORIGIN      
        1 cgccaagcca ccgtactgtg aagcagcctg gacttgcgac gcctgggttc tgctaggcag
       61 gagcgccatc cacatgtctg cggctactcg atgccaatgg atgttgctgc gctcgccaag
      121 gcgtgccatc gatacggctg aaccactcca tcgtccttct gagctgcaag tggaatcgcg
      181 agcgtgccga acctaatcga tggccacgag tccacgggcc aggtagggtc ggccaactcg
      241 aacaaggagc tcgcaatgcc ggtcgtacta atccaccagg gtccgacggt caccgcggag
      301 aactaccgcg ccaccgtcca gaagctcgcc ggcaaggaca agatggagtc agcgtcggac
      361 tggcccgtcg ggggcatcct ttcccactca gccggcgaaa gcccgagcgg attccgcgtc
      421 gtcgacgtct gggagtccca ggaggcgtgc gacgcgttcg gccagcacct gggaccgatc
      481 ctcgaagagg tcggcatcac ggatccgccg gagatgtacc cagcggagac cttcgtttcg
      541 agctagtccc gcagcttcgc caagagcggc gcgtggtccg agtgcttctg gcctttgcgg
      601 aagtcgcggt cgatcaggct ggctcgagac gctctgccag gggcggagaa ggaggaagcg
      661 tcgagccgca ggccgagctt ctcgtggaag tggcaggccc ggacaaccgc tgatcaatcc
      721 ttacgaaagt tccgctttcg cgtgcacgga gtcgtctccg tcgagtcccg cggcgctctc
      781 gcaagggacg agcatcgagc gtaaagctca cggcgcttcg aagcgctcga gcgcggcctt
      841 cattgtcgca agcgcgttcc aggcgttcgg gaaaccggcg tacacagcca cctgacgcag
      901 cacctcgcga atctcctcct cgctcgcccc ctggcggcgg gcggcctgga catagacgcc
      961 cagttgcagc tgctgatcaa gggaggcgag catcgcgacc gtgcacagcg cacgagtctt
     1021 cgggtccagc acctctcccg cgtagacctc gccgtaaacg aagtcgatga tgtagcgatg
     1081 gatctccggg tctagctgct cgaggtagtc gcggccgcgc tccacattct cggcgcccac
     1141 gaaacgcgcc atcacatccg ggccgggctc gctcatcgcg acgctccctg accggcgatg
     1201 taggactgcg ggtcgaaggc gacccggatg cccgtcgagg caaacttgcc cacgacctcg
     1261 ccgtcggcga ttacgtcggc ctcggcccac acgagtcggc cgccggcgcg tatcaccttc
     1321 gcctcggcgc ggaggcgttc gattttcttg gcgttcaccg ggcggatgaa gcgagcgaag
     1381 atgtcgatcg tcgtgatcgc gctcttctga acctcctcgt cggtgaggat gctcgtcgtc
     1441 gcggccgcca tggcggcgtc cgcgagcgcg gtggcgaccc ctccttggat gacaccgagc
     1501 gggttgccca gctcctcgcg ggccgccatc tcgtagatcg cctcaccgcg accgaactcc
     1561 accagctgca tgtcgagcgt ctcggagacc ggcgagcgca gctttcccgc cttggtctgc
     1621 tccatcatct gatcgacgaa ttcgtagctc atccgggcga tattacccag gagtaatatt
     1681 tgtactcatg acggtgcgcg accgctctcc caagcgccac aggaccggat cgcgcggcgt
     1741 gccccgcgct gttcgcgaac ccctgattct cgacgtcgca gggcgggtct tcgcctcacg
     1801 gggctaccac gcggcatcca tggacgagat cgcggcggag gcagacgtca cgaagccgct
     1861 gatctacgcc tacttcggct caaaggaggg cctctacatc gcctacatcg agcggtcggg
     1921 gcgcgagctg ctcgagcgca tgcgcaccgc tacccagccc acagcaccgc cgcgcgaacg
     1981 gctgcaaacc ggcatcctcg agttctttcg cttcgtcggc gagcgccgtg acggctggca
     2041 ggtcctctat tccgaggccg cctcgcgtgg aggcccgctg gcggaggagg tcgcgaccct
     2101 ccgtgcggcc atcgcacgga tgatcacacg gctcctgatg gagacgatcc cggacctgcg
     2161 cgcctcggac gagacgaccg ccgccctcga cgcggtcgcc cacgcaatcg tcggagcggg
     2221 agagtcactc gcgaaccgat cactcgccca ccccgagctc ccgcccgagc gggcggccca
     2281 gctgctcgtc gccatagggc agaccggcgt cgagcagacg gtggccgcgc tcgatagcag
     2341 accggcgggc tcggcctcgt tgcccagctc cccgtcgatg tctgccagct cagcgacgac
     2401 cgcggggtcc ggccggtaag cgcgcaagct cgagcacgtc gagttaggcg ccgaagcgta
     2461 gtgccagtcc tcccacgagt acctcctcac gaacccgaat cgctgggagt tcgccgccag
     2521 ccagccgtac gcggccggcg gcccaagatc tgacacgcgg agtatgatcg cgccaggggt
     2581 gctcgggcca ggtccagcct gatccggatt tcggcgtccc cgctcaggtc tggctcgatg
     2641 ctcgcgcccg accccgccga gcggcgagac gccgacctcg gcgcccaacc gatggatttc
     2701 tgaccctcga cgtatctctg ccgtcaaagg ggcgatgtcc cgccccggga aacgaccaga
     2761 gagcgaggat cagatgacca tgaacatgcc cccgaccgtg tcgccgcagg agtggaaggc
     2821 ggcgcgcgag gaactgctcg tcgaggagaa ggagctgacc cacgcccgtg acgcgctggc
     2881 cgccaagcgc cgccgcatgc cgagaatggc cgtcgagaac gactatgagt tcgagggccc
     2941 gaacggcccc gcgagcctgc tcgacctgtt cgaggggcgc cggcagctga tcgtctaccg
     3001 cttcttcttc gagccgggcg tccacggctg gccggacggc ggctgccccg gctgctcctg
     3061 gattgccgat cagatcacgc atcccgccca tctgaacgcc cgcggcacca cgattgcgct
     3121 gatctcgcgc gccccgcaga cggacatcgg gcgctggaag gcgcggatgg gctgggagat
     3181 ccttccgtgg tacacgctga ccgacgactt cgacaccgac ttcgacgtcg gcgagtggca
     3241 cggcaccaac gtcttctatc gcgacgacga cgatcggatc ttccgcacct acttcgtcga
     3301 cggccgcggc gacgaggcgc tgggcggtac ctgggcgtac ctcgacatca ccgcgct
//
LOCUS       DASVEU010000027         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_409935,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000027 DASVEU010000000
VERSION     DASVEU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409935"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF225_01100"
     CDS             complement(<1..435)
                     /locus_tag="VF225_01100"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143879.1"
                     /translation="MTDEHVLDIARRDVTVSSPDKAFFPERGETKLDLVRYYLAVGEP
                     FLRAARGRPTLMQRFPNGAEGKSFFQKRVPEGAPEWLQTTIVSTPNGTTSRALVLADM
                     AHVVWAVNLGCLGFHLWPYLRDRPDVADELRLDIDPQPGVGFD"
     gene            814..1113
                     /locus_tag="VF225_01105"
     CDS             814..1113
                     /locus_tag="VF225_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143880.1"
                     /translation="MAKLAKTYSTCLRSLRRAGDPGRFRPAARIAERACQQLERAGSR
                     MEEAVRIDSAGIDSQAEADRYNSLVQRALEGQGNAVNAFAQASARARAIEQELAD"
     gene            1275..2186
                     /locus_tag="VF225_01110"
     CDS             1275..2186
                     /locus_tag="VF225_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEX7143881.1"
                     /translation="MRRVKLIATLALALAVYAAAMVGTASGGLAREAAQVWNCDPEAS
                     TLVGRGGVVREPALAEVHADLPASAKGKAGKSFRATVPVYFHVVTDGSVGNLTAAQIA
                     DQISVLNVTFGGGEGGARTGFTFELAGVTRTDNARWFYSGSGGAEHDIKRTLRRGDGK
                     ALNLYSTTAGAYLGWAYLPSILTSPSQAYLDVVVDWESMRGTSDAYAGEFDQGETATH
                     EVGHWLNLEHTFFNGCSKRGDFVADTPPQKTPTSGCPEGKDTCPEPGLDPIHNYMDYS
                     FDSCYTEFTPGQAQRMKDAWLFYRAGG"
BASE COUNT          378 a          740 c          779 g          320 t
ORIGIN      
        1 gtcgaagccg acaccaggct gcgggtcgat gtcgaggcgc agctcgtccg cgacgtccgg
       61 acggtcacgg agatatggcc agaggtgaaa gccgaggcag cccaggttca ccgcccagac
      121 cacatgcgcc atatcggcca gcacgagcgc gcgggaggtc gtcccgttcg gcgtgctgac
      181 gatcgtcgtt tgcagccact ccggcgcgcc ttcggggacg cgcttctgga agaacgactt
      241 gccctccgcg ccgttgggga agcgctgcat gagggtgggc cggccacgcg cggcgcgtag
      301 gaacggctcg ccgactgcga ggtagtagcg caccaggtcg agcttcgtct ccccgcgctc
      361 cggaaagaac gctttgtccg ggctcgagac cgtcacgtcg cgcctcgcga tgtcgagcac
      421 gtgctcgtct gtcaccaggc ccgaaccgta gccggggcga aggagtcgtg ccggcccttg
      481 aaacgcctcc ttggacgagc taccgtcgtt cgcttgttgc gttcgttaca gataatcggg
      541 ctcgccaccg ccttcctgct cccggcttcc ttgctgatcg tggcgtgctc tggcgatggc
      601 gaacagggag cgcctgcgac ggtcgaagcg accacggcgt cagacgggac caccgagccg
      661 ggaaccagcg aggcgacaac ggaggagacg acgaccgaga cgggtccgct gccgataacg
      721 gcttccgagc agcgttggac gcgggagatg aacgagctcc gccgacagat gacccgcggt
      781 ttccaccaaa cgagggtcta caccaactcg acgatggcca agctcgcgaa gacctactcc
      841 acgtgtctcc gctcgctccg tcgtgctggg gatcccggcc gctttcgtcc ggccgcccgc
      901 atcgcggagc gcgcgtgcca gcagctggaa cgtgccggga gcaggatgga ggaagcggtg
      961 cggatcgact ccgcggggat cgattcgcag gcggaggccg acaggtacaa ctcgctcgtc
     1021 caacgcgcgt tggagggtca ggggaatgcg gtcaacgcct tcgcccaggc gagtgcgcgc
     1081 gcgcgggcga tcgagcagga gctagccgac tgagccccaa tgcggggggc cgcccgcccg
     1141 ggccgccccg cgagcgaaaa tggccgtgct cgagccgaca gatcggtcct tcgagccgtg
     1201 tttcctgcta caacccgtcg gccgaggcca ggcccggctc ggcggcatta cgaacgcgaa
     1261 cgagaggacg ggacatgagg cgagtcaagc tgatcgcgac gctcgccctc gccttggcgg
     1321 tgtacgcggc cgccatggtc gggacggcga gcggcggact ggcacgggag gcagcgcagg
     1381 tctggaactg cgatccggag gcctccacgc tagtcgggcg aggcggcgtc gtccgcgagc
     1441 ccgccctggc tgaggttcac gctgatctgc cggcctcggc caagggcaag gcgggcaaga
     1501 gcttcagggc gaccgtgccg gtgtacttcc acgtggtgac cgacggatcg gtcgggaacc
     1561 tcacggctgc gcagatcgcc gaccagatca gcgtgctgaa cgtgaccttc ggcggcggcg
     1621 agggtggtgc cagaaccggc ttcacgttcg agctcgccgg cgtcacgcgc acggacaacg
     1681 ccaggtggtt ctactccgga tccggcggcg ccgagcacga catcaagagg acgctccgcc
     1741 gcggcgacgg caaggcgctc aacctgtact cgacgacggc cggcgcctat ctcgggtggg
     1801 cctacctgcc ctcgatcctg acgtcgccgt cgcaggccta cctggacgtc gtcgtcgact
     1861 gggagtcgat gcggggcacg tcggacgcct acgcaggaga gttcgaccag ggcgagaccg
     1921 cgacccacga ggtcggccac tggctgaacc tcgagcacac cttcttcaac ggctgcagca
     1981 agcggggcga cttcgtcgcc gacacgccgc cgcagaagac ccccacgtcc gggtgtcccg
     2041 aggggaagga cacctgcccg gagcccgggc tcgacccgat ccacaactac atggactact
     2101 cgttcgactc ctgctacacg gagttcaccc ctggtcaggc gcagcggatg aaggacgcct
     2161 ggctcttcta ccgcgcgggc ggctagcggt cagggattcg gagagcggcg ggccgga
//
LOCUS       DASVEU010000028         9303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_215557,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000028 DASVEU010000000
VERSION     DASVEU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215557"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF225_01115"
     CDS             complement(<1..402)
                     /locus_tag="VF225_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7143882.1"
                     /translation="MGAGKSTIGQEVASLSGRPFVDLDGEIERRHGPVGELFVRGEPE
                     FRRIEEAVAAEVLASSTPSVIALGGGAVLSGGTRELLRTDAFTLWVDVDVAAAWKRVR
                     DTGRPLAQDEVEFRRLFDERQPLYEAVSARLV"
     gene            complement(438..1517)
                     /locus_tag="VF225_01120"
     CDS             complement(438..1517)
                     /locus_tag="VF225_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7143883.1"
                     /translation="MGLQLATAGESHGPALVAIVSGLPAGLVLEKAAIDADLHRRQQG
                     YGRSPRQQIERDEVEVLAGLRHGRTLGTPLALVVHNRDHRNWEWGMSPWQPEGEPSGK
                     GTKEVTLPRPGHADLAGAFKYGLSDVRDVLERASARHTAVVVAAGSVAKALLSEIGVA
                     VAGHVLSADLEQRVDEARADRDTVGGVVEVRARGVPPGLGSYATKDDRLDARLAATLM
                     GTQAVKGVEIGEGFELASKRGSAAHDEILRDQRGLYRETNRAGGLEGGVSNGEEIVVR
                     AAMKPLPTLMKPLASVDLQTGEPAQALVERSDTAAVEALSVVAEACVAWELARAAKEK
                     FGGDALGDFLAAHRAYLERIDWSPR"
     gene            complement(1517..2197)
                     /locus_tag="VF225_01125"
     CDS             complement(1517..2197)
                     /locus_tag="VF225_01125"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143884.1"
                     /translation="MISGETRLVGLLGDPVAQSLSPLMQNAAFAARGLDWAYVPLPVA
                     AERLADAVKGLVALDFAGANVTTPHKLTVAKLVDTDESSANTLVVRDGRVFGSSTDAA
                     ILGELEFRRAVIVGDGGAAVAFASALPGAPRFSRRGNWPPEVSDADVIINATSERDQS
                     LFELGPGQTLVDLPYPPTATARAAEAASARVVDGLDVLVAQGAASFELWTGMPAPVDV
                     MRAAVRSA"
     gene            complement(2194..3186)
                     /gene="mltG"
                     /locus_tag="VF225_01130"
     CDS             complement(2194..3186)
                     /gene="mltG"
                     /locus_tag="VF225_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7143885.1"
                     /translation="MGEQRPGVIPPRQGRPPQGPDPRQVARRRVLALGVLVLILGGLG
                     WLAVAAIGGGTSSDQAAQSSTLSSTLVYRTVTRTTATGTVETVIAMPKPFHVVFPEGF
                     TRKQMAERVAAVATIAKHKRKVQPRLSRAAYLSSTAKPRVPPCFRNKVQTVEGFLFPA
                     TYEFFKDTTSAQLVQDQLDAFCENWKQLDLGYARSKNLTPYDVLIIASMVEKETLSPD
                     ERQLVSAVIYNRLHAGMPLGIDATLRYGLDIPPTESIHESQLQSDSPYNTRKRKGLPP
                     TPIASPGLASLQAAAHPAKVKYLYFVRKPDKKHHFFTASYQAFQNYANAHGYGP"
     gene            complement(3162..3557)
                     /gene="ruvX"
                     /locus_tag="VF225_01135"
     CDS             complement(3162..3557)
                     /gene="ruvX"
                     /locus_tag="VF225_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7143886.1"
                     /translation="MKVLALDYGRARTGVAVSDPTGTIARPLCVVAEAATGDGLARLA
                     ALIGEQEVDRVVVGLPLTLRGEHGEQARETERFVDALTDVTNVPVVTFDERFTTDLAE
                     QTPAEAPEDARAAAHLLSGYLTWASSAPA"
     gene            complement(3554..6091)
                     /gene="alaS"
                     /locus_tag="VF225_01140"
     CDS             complement(3554..6091)
                     /gene="alaS"
                     /locus_tag="VF225_01140"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7143887.1"
                     /translation="MHTSAELRAGYRAFFESKGHVFRPSAPLIPRADDRSTLLTTAGM
                     QPLMPYFLGREAPPAPLLTTVQKCFRTPDIDEVGLDGSHLTFFEMLGNFSFGQYFKEG
                     AIDLAWEFVFEHLKIDSERFWVSVFAGDPELGLGEDEVAYDHWVKIGQPTERIVFLPA
                     TENFWSVGGPGPCGPDTEMYYDWGEEHGCGEPDCQPSCTRCERFLEFWNLVFMEFELH
                     VDGKLTPLPKQNVDTGMGVERTAAILQDVMTVYETDVYQAIMGWIAEQSGVAYGDSPE
                     ATKAHRVLADHGRGMTFLAADGIAPSNEGRGYVMRRIIRRAVQQGARVGLEPPYLAPL
                     ADVVIEQMQAGYPELEEHRDEIHRVLSAEEERFGKTLERGLRLFEEVAEKDEAISGEE
                     AFRLHDTYGFPLELTRELARERGLGVNEEEFTRLMELQRTRSRGAISKDDKRAAEFAQ
                     RAGFRSEFVGYEKTEVLTQIGALEDLGDRLFLAKLRESPFYPEGGGQVTDAGWIERDD
                     GSGVRAELREAYRLEEDQALLFEGDGFSEGDRVRAVVAWSVRFPTMANHTGTHLLHKA
                     LRDVLGEHVKQAGSAVRPDKLRFDFTHPQQLTDEERERVEHLVNEKVFENLPVRAFVT
                     PIDEARKLGAMMLFGEKYGDHVRVVEIPGFSRELCGGTHVRSTAEIGPFVILSEGSVG
                     AGARRIEAITSGEAHAYLHATRRDADELRAELERVRKESKKPKEAAAADFEIVDNEGG
                     VVLAEAKSLKGGALRDLSDRLRQQEKAAGVIVGSVDDGRVYLVVNLDESLVGRGLDAS
                     KLVRDLGRHIEGGGGGRPTLGEAGGKNPAGLRSALEAGKQAIAAALE"
     gene            complement(6113..6829)
                     /locus_tag="VF225_01145"
     CDS             complement(6113..6829)
                     /locus_tag="VF225_01145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7143888.1"
                     /translation="MTQPIDEAAFVRAQYETEENLRARKSAYVNAEGDDPREFAFEAI
                     AEAKPRKVLEVGGGEGELAERIRDELAAEVIAIDQSERMVEIQRSKGIDARVGDVEEL
                     PFANGEFEVVVAAWMLYHVPDLDRALAEIARVLKPGGRLVAVTNAIDHLQELWDLARR
                     ETSLRRFTFRSENGEESLRRHFASVSRRDARGWTTMDDEAVRRFGASWGDLAPLVTAG
                     PLDEPLRVRRHSTIFVAETA"
     gene            complement(6826..7596)
                     /locus_tag="VF225_01150"
     CDS             complement(6826..7596)
                     /locus_tag="VF225_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX7143889.1"
                     /translation="MSEADKALLRRYHEDGDLQAREQLIEQYMSLVRSLARRYSYRGE
                     QLEDLVQIGAIGLIKAIDRFDIDRGVELTTYATPNIIGEIKRHFRDKGWSVRVPRGLQ
                     ELNVQLSKLVEQLTVQLGRSPTIAELAKGAGVEEEEVLEALESGRAYSSLSLSSGGGA
                     NDDGEELDPLESIGTEEHQYDVSEDRAVLAPGFRVLDERERRILHLRFFEGLTQSQIA
                     QQVGISQMHVSRLIRRSLEKIRDEIAAEEGSEPKVARG"
     gene            complement(7593..8030)
                     /locus_tag="VF225_01155"
     CDS             complement(7593..8030)
                     /locus_tag="VF225_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143890.1"
                     /translation="MMTVVRSTQWIELEGDVIADEIRLTLPADDAFHGVAHLVLGGLA
                     ARLNLSFENLEDLELALDSLLERPSDGREVTLRVLVEDGELRMIVGPFTAVRAELGEG
                     GADSLNLGRILGAVCDSVEIEDRDGSEWVVLTKQLDALPGGRG"
     gene            complement(8081..>9303)
                     /gene="mnmA"
                     /locus_tag="VF225_01160"
     CDS             complement(8081..>9303)
                     /gene="mnmA"
                     /locus_tag="VF225_01160"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7143891.1"
                     /translation="RGCDFALVRLAFDGDRIVEADASGLDRSLVGLTLLEAASVGGET
                     LAVDALANAIGPVFRASRSPGRVAVAMSGGVDSAVALLKSLPNAVGVTLRLWLDPDGP
                     DSERACCSPDAVLAARATCHSLGVPHVTLDLREEFRRAVVDPFVRGYERGETPNPCIQ
                     CNGGFRFAQLLAFAHRAGADKLATGHYARIVEHRGKLLLARGLDPAKDQSYMLARVDP
                     AKLDRISFPLGAQTKAETRAEAERAGLEAAGRGESQEACFLAGDDYRSFLERRGLPAQ
                     TGSIVDEDGHELGKHDGYWRFTPGQRKGLGVAAPEPLYAIRSDATTNTVLVGRLEALA
                     RTTIEASGRLYVPVQRAQAKLRYRSDAVAAHIHERDGGFRLELDRPAYGVAAGQAAVL
                     YEDDVIVGAGLIRE"
BASE COUNT         1533 a         3219 c         2996 g         1555 t
ORIGIN      
        1 gacgaggcgc gccgagaccg cttcgtacag cggttgcctc tcgtcgaaca gacgcctgaa
       61 ctcgacctcg tcctgcgcca gcgggcggcc ggtgtcgcgg acgcgtttcc acgctgcggc
      121 cacgtcgaca tccacccaca gggtgaacgc gtcggtccgc agcagctcgc gtgtgccgcc
      181 cgagagaacg gcgccacccc ccaaggcgat caccgacggt gtcgaggaag caaggacctc
      241 cgcggcgacc gcttcttcga tgcggcggaa ctcgggctcg cctcgaacga acaactcgcc
      301 gaccggacca tgtcgccgct cgatctcacc atccaggtcg acaaacggcc tgccgctgag
      361 gctcgcgacc tcctggccga tcgtcgactt tcccgccccc atgaagccca cgagcgcgag
      421 atgacggtcg agcgcgccta ccgtggtgac cagtcgattc gctccaggta tgcgcggtgc
      481 gcggcgagga agtcgccgag cgcgtcgccg ccgaacttct cctttgcggc acgcgcaagc
      541 tcccatgcca cgcacgcctc cgcgacgact gaaagcgcct cgaccgcggc ggtgtcgctg
      601 cgttcgacga gcgcctgcgc cggctcgccc gtctgcaggt cgaccgacgc cagcggcttc
      661 atcagggtcg gcaacggctt catggccgcg cgcacgacga tctcctcccc attcgagacg
      721 ccaccctcga gcccgccggc gcggttcgtc tcgcggtaga gcccgcgctg gtcgcggagg
      781 atctcgtcgt gtgcagcgga gccccgcttc gacgccagct cgaacccttc gccgatctcg
      841 acacccttca cggcctgtgt gcccatcaga gtcgcggcca ggcgagcatc gagacggtcg
      901 tccttcgtgg cgtaagaccc gagaccggga ggcacgcctc gggcgcgaac ctcgaccacg
      961 ccgccgacgg tgtcccgatc tgcgcgcgct tcgtcgacgc gttgctcgag atcggcgctg
     1021 agcacgtgcc ccgcgacagc gacgccgatc tcggagagca aagccttcgc cacggaaccc
     1081 gctgcgacga ctacggcggt atggcgcgcg ctggcacgtt cgagcacgtc ccggacgtcg
     1141 gacaggccgt acttgaacgc acccgcaagg tccgcatggc cgggccgggg caacgtcact
     1201 tctttcgtgc ccttccctga aggctcgccc tccggctgcc aggggctcat cccccactcc
     1261 cagttcctgt gatcgcggtt gtgcacgacg agggcgagcg gcgttccgag cgtgcgaccg
     1321 tggcggagac ccgcgagcac ctccacctcg tcgcgctcga tctgctgacg cggactgcga
     1381 ccgtagccct gctggcgccg gtggaggtcg gcatcgatcg ccgccttctc cagcacgagc
     1441 cctgccggca gtccggacac gatcgcgacg agcgcaggcc cgtgtgattc acctgcagtt
     1501 gcaagctgca gtcccatcac gcggagcgta ctgccgcgcg catcacgtcg accggtgcag
     1561 gcatgccggt ccagagctcg aacgaggccg cgccctgggc aacgagaacg tcgagcccgt
     1621 cgacgacgcg cgctgatgca gcctcggccg cgcgcgcggt ggccgtcggc ggatacggaa
     1681 ggtcgacgag tgtctgtccg ggaccgagct cgaagaggct ttggtcgcgc tcggacgtgg
     1741 cgttgatgat cacgtcggca tccgagacct ccggtggcca gtttccgcgg cgtgagaacc
     1801 gcggtgcgcc cggcaacgcc gacgcgaagg cgacggccgc accgccgtcc ccgacgatca
     1861 cggctcgccg aaactcgagc tctccgagga tcgctgcgtc cgtcgaagag ccgaagacgc
     1921 gaccgtcgcg aacgaccagc gtgttcgcgg acgactcgtc ggtgtcgaca agctttgcca
     1981 cggtcagctt gtgcggcgtc gtcacgttcg cccccgcgaa gtcgagcgcg accagaccct
     2041 tcacggcgtc cgccagccgc tcggcagcga cgggcagcgg cacgtacgcc cagtcgagcc
     2101 ctcgcgccgc aaaggctgcg ttctgcatca gcggcgacag cgactgggca accggatcgc
     2161 cgagcaaccc gacaagccgt gtctcacccg agatcacggg ccgtagccgt gggcgttcgc
     2221 gtagttctgg aacgcttggt agctcgcggt gaagaagtga tgcttcttgt caggcttgcg
     2281 gacgaaatag aggtacttca cctttgccgg atgcgccgcc gcctgtaagg aagcgagccc
     2341 ggggcttgcg atgggcgtcg gcggcagccc tttgcgctta cgtgtgttgt agggcgagtc
     2401 actctggagc tgcgactcgt ggatcgactc ggtcgggggg atgtcgaggc cgtaccgaag
     2461 cgttgcgtcg atgcccagcg gcatgccggc atggaggcgg ttgtagatca ccgctgagac
     2521 gagctggcgc tcatcgggcg agagcgtttc cttctcgacc atcgacgcga tgatcagcac
     2581 gtcgtagggc gtcaggttct tggagcgggc gtagccgaga tcgagctgtt tccagttctc
     2641 gcaaaaggcg tcgagctggt cctgcacgag ttgggccgag gtcgtgtcct tgaagaattc
     2701 atatgtcgcc gggaagagga agccctcgac ggtctgcacc ttgttccgga agcagggtgg
     2761 cacgcgcggc ttggccgtcg acgagaggta agcggcccgc gacaggcgtg gctggacctt
     2821 tcgcttgtgc ttcgcgatcg tcgccaccgc ggcgacgcgt tctgccatct gcttgcgcgt
     2881 aaagccctcc gggaagacca cgtggaacgg cttcggcatt gcgatcacgg tctcgacggt
     2941 ccctgtcgcg gtcgtgcggg tcacggtgcg gtacacgagc gtgctggaga gggtcgaaga
     3001 ctgcgcggcc tggtcgctgc tggtcccgcc gccgatcgcg gcaacggcaa gccacccgag
     3061 cccgccgagg atgagcacga gcactccgag cgccagtacg cgccgccttg cgacctgacg
     3121 agggtcgggc ccttgcggcg ggcgcccctg ccgcggcgga attacgccgg ggcgctgctc
     3181 gcccacgtca agtatccgga gagaagatgc gctgccgccc gcgcgtcttc gggggcctcg
     3241 gcaggcgtct gttcggcgag gtcggtcgta aagcgttcgt cgaaggtgac gacaggaacg
     3301 ttcgtcacgt cggtgagagc gtcgacgaag cgctccgtct cgcgcgcctg ctccccgtgt
     3361 tcgccgcgca gggtgagcgg gagccctacc accacgcggt cgacctcctg ctcgccgatc
     3421 agcgccgcca gacgagcgag gccgtcaccg gtcgcagcct ccgcgacgac gcagagcggt
     3481 cgtgcaatgg tgccggtcgg atccgagacg gcgacgccgg tgcgcgcccg gccgtagtcc
     3541 agtgcgagaa ccttcactcg agcgctgcgg cgatcgcttg cttcccggct tccagcgcgc
     3601 ttcgcaatcc ggcgggattc ttgccgcccg cctcaccaag cgtgggccgt cctcctccgc
     3661 ctccctcgat gtgccggcca aggtctcgca cgagcttcga cgcatcgagg ccgcggccca
     3721 cgagtgattc gtcgaggttg acgaccagat agacgcgccc atcgtcgacg gagccaacga
     3781 tcaccccggc cgccttctcc tgctggcgta ggcgatccga taggtcgcgc agcgctccgc
     3841 ccttgagtga ctttgcctcc gcaagaacga cgcctccctc attgtccacg atctcgaagt
     3901 ctgcggcggc cgcctccttc ggcttcttcg actctttccg gacccgctcg agctccgcac
     3961 gcagctcgtc tgcatcacgt cttgtcgcgt ggaggtacgc atgtgcctcg ccggaggtga
     4021 tcgcctcgat tcgccgcgcg cctgcgccaa ccgaaccctc ggagaggatc acgaatggtc
     4081 cgatctccgc cgtcgacctg acgtgcgtgc ctccgcacag ctcgcgcgag aagccgggaa
     4141 tctcgacgac gcgcacatga tcgccgtact tctcgccgaa cagcatcatc gcgccgagct
     4201 tgcgagcctc atcgatcggc gtcacgaagg cgcgtaccgg cagattctcg aagaccttct
     4261 cgttgaccag gtgctcgact cgttcccgct cttcgtccgt gagctgctgc ggatgcgtga
     4321 aatcgaaacg aagcttgtcc ggtcgcacgg cagaccccgc ctgcttgaca tgctcgccga
     4381 gcacgtcacg tagcgccttg tgcagcaagt gcgtcccggt gtgattcgcc atcgtcggga
     4441 accggacgct ccaggccacg accgctcgaa cccggtcgcc ttccgagaac ccgtcaccct
     4501 cgaagagcag tgcctgatct tcctccagcc gatacgcctc ccggagctcc gcgcggacgc
     4561 ctgaaccatc gtcgcgctcg atccagccgg cgtcggtgac ctggccgcca ccctcgggat
     4621 agaaaggcga ttcgcgtagc ttcgcgagga agagcctgtc gccaagatcc tcgagtgcac
     4681 cgatctgcgt gagcacctcg gtcttctcat aacccacgaa ctcgctgcga aagccggcgc
     4741 gctgggcgaa ctcggctgcc cgcttgtcgt ccttcgagat cgcgccacgg gaacgcgtgc
     4801 gctgcagctc catcaaccgc gtgaactcct cttcgttgac gccaaggccg cgctcgcgtg
     4861 caagctcgcg ggtcaactcg agcgggaagc cgtaggtgtc gtgcaggcgg aacgcttctt
     4921 cgccagagat ggcctcgtcc ttctctgcaa cctcctcgaa gagtcgcagt ccgcgctcga
     4981 gtgtcttccc gaaacgctcc tcctctgcgc tgagaacacg gtgaatttcg tcgcggtgct
     5041 cctcgagctc gggatagccg gcctgcatct gctcgatcac gacgtccgcg agaggtgcga
     5101 ggtacggcgg ctcgagcccg acgcgagccc cttgttgcac ggcacgacgg atgatccggc
     5161 gcatcacgta accccggcct tcgttcgacg gcgcaatgcc gtcggcggcc aggaacgtca
     5221 tgccgcgacc gtgatctgcg agcacgcggt gcgccttcgt cgcctccggg gaatcgccgt
     5281 acgcgacacc gctctgctcc gcgatccagc ccatgatcgc ctggtagacg tcagtctcgt
     5341 agacggtcat gacgtcctgc aggatcgccg cagtccgttc gacacccata cctgtgtcga
     5401 cgttctgctt cgggaggggc gtcagcttgc cgtcgacgtg gagctcgaac tccatgaaga
     5461 cgaggttcca gaattccagg aagcgctcgc agcgcgtgca gctcggctgg cagtcgggct
     5521 ccccgcagcc gtgctcttcg ccccagtcgt agtacatctc ggtgtccggg ccgcaggggc
     5581 ccgggccgcc gaccgaccag aagttctcgg tcgccgggag aaacacgatt cgctcggtcg
     5641 gctgtccgat cttcacccag tggtcgtagg caacctcgtc ttccccgagc ccgagctccg
     5701 gatctcccgc gaacacactg acccagaaac gctccgagtc gattttcaag tgttcgaaga
     5761 caaactccca ggcaaggtcg atcgcgcctt ccttgaagta ctgcccgaac gaaaagttgc
     5821 cgagcatctc gaaaaaggtc aggtgcgagc catcgaggcc cacctcgtcg atgtccggcg
     5881 tgcggaagca tttctgcacc gtggtgagca acggcgccgg cggtgcctca cgccctagga
     5941 aatagggcat caggggctgc attccggccg ttgtcagcag cgtcgagcgg tcgtccgcac
     6001 gcgggatcag cggcgcggag ggccggaaga cgtggccctt ggactcgaaa aaagcgcggt
     6061 atcccgcgcg cagctcagcc gacgtgtgca tgcctcgatt gtaggggtgc ggtcaggcgg
     6121 tctcggcgac gaagatcgtg ctgtgccgcc ggacccggag cggctcgtcg agtgggccgg
     6181 ccgtcacgag aggcgccagg tctccccaac tcgcaccgaa tctccggacc gcctcgtcgt
     6241 ccatcgtcgt ccagccacgc gcatcgcggc ggctgacaga cgcaaagtga cgccggagcg
     6301 attcttcgcc gttctcgcta cgaaacgtga acctgcggag cgacgtctcg cgtcgggcga
     6361 ggtcccagag ctcttgcaga tggtcgatcg cgttcgtgac agcgacgagg cgcccgcccg
     6421 gcttcaagac tcgtgcgatc tcagcgagag cgcgatcgag atcgggcacg tggtacagca
     6481 tccatgcagc caccacgacc tcgaactcgc cgttggcaaa aggaagttcc tcgacgtcac
     6541 cgacacgcgc gtcgattccc ttcgagcgct ggatctcgac catgcgctcc gactggtcga
     6601 tggcgatcac ctctgcggcg agctcgtcgc gaatccgctc ggccagctct ccttcgccgc
     6661 cgccgacttc gagcaccttc cgcggcttcg cttccgcaat ggcttcgaat gcgaattcgc
     6721 gaggatcgtc gccctcggcg ttcacgtaag cagacttgcg ggccctgaga ttttcttcgg
     6781 tctcgtactg ggcccggacg aaggcagcct cgtcgatggg ctgggtcaac ctctcgcgac
     6841 tttcggctcg ctgccctcct cggcggcgat ctcgtcgcgg atcttctcga gcgagcgccg
     6901 gatcagccgc gacacgtgca tctgcgagat cccgacctgc tgagcgatct gggactgcgt
     6961 caatccttcg aagaagcgca gatggagaat cctgcgctcc cgttcgtcga gcacgcggaa
     7021 cccgggtgcg agcactgcac ggtcctccga cacgtcgtac tggtgctctt cggtgccgat
     7081 cgactcgagc ggatcgagct cctccccgtc gtcgtttgcc ccgccacccg aggagagcga
     7141 cagcgacgag taggcgcgac cggactcgag tgcctcgaga acctcttcct cttcgacgcc
     7201 ggcccccttt gcgagctccg cgatcgtggg cgagcggccc agctgcaccg tcagctgctc
     7261 gacgagtttg gaaagttgca cgttgagctc ctggagcccg cgcgggacgc ggaccgacca
     7321 acccttgtcg cggaagtgcc gcttgatctc cccgatgatg ttcggcgtcg cgtaggtggt
     7381 cagctcgacg ccgcggtcga tgtcgaaccg gtcgatcgcc ttgatcaatc cgatcgcccc
     7441 gatctggacg aggtcttcga gctgctcgcc gcgatacgag taccgccgcg cgagcgagcg
     7501 cacgagcgac atgtactgct cgatcagctg ctcgcgcgcc tgcaggtcgc cgtcctcgtg
     7561 gtagcgacgg agaagtgcct tgtcggcttc gctcacccac gacctcctgg tagagcgtcg
     7621 agctgcttcg tcagaacgac ccattccgag ccgtctcggt cctcgatctc cacggagtca
     7681 cagacggcgc cgaggatccg gccaaggttg agtgaatccg cgcccccttc cccgagctcg
     7741 gcgcgcacgg cggtgaacgg gccgacgatc atccggagct caccgtcctc gacgaggacc
     7801 cgaagggtca cctccctgcc gtcgcttgga cgctcgagca gcgagtcgag cgcaagctcg
     7861 aggtcctcga gattctcgaa gctcaggttc agccgcgcgg cgagcccacc cagcacgaga
     7921 tgggcgacac cgtggaaggc gtcgtccgca ggcagcgtca agcggatctc gtcagcgatc
     7981 acgtctccct ccagttcgat ccactgggta gaacgaacaa ccgtcatcac cggttgcgca
     8041 ggtgagcgct aggacctcca ccctgttcgg atactacggt tcactctcga atcagcccgg
     8101 ccccgacgat cacatcgtcc tcgtagagga ctgccgcctg ccctgcggca acgccgtagg
     8161 cgggtcggtc gagctcgagg cgaaaaccgc cgtcgcgctc atggatgtgc gcagcaacgg
     8221 cgtccgaccg ataacggagc ttcgcctgcg cgcgctgcac gggcacgtag agccgccccg
     8281 aggcttcgat cgtggtgcgg gcgagcgctt cgagccgccc gacgagaacg gtgttcgttg
     8341 ttgcatccga acgaatcgca taaagcggct ctggcgccgc gactccgagg cccttccgct
     8401 ggccgggagt gaaccgccag taaccgtcgt gcttgccgag ctcgtgtccg tcttcgtcga
     8461 cgatcgagcc cgtctgtgcc gggagcccgc gacgctcgag gaacgagcgg tagtcgtcgc
     8521 ccgcgaggaa acacgcctct tgactctcgc cgcggcccgc cgcttccagg ccggcgcgct
     8581 cggcctcggc gcgcgtctcc gccttggtct gggcgccgag tgggaacgag atccggtcga
     8641 gcttcgctgg gtcgacccgg gccagcatgt acgactggtc cttcgctgga tcgagcccgc
     8701 gcgcgagcag gagcttgccg cgatgctcga cgatccgtgc gtagtgcccg gttgcaagct
     8761 tgtccgcgcc cgcacggtgt gcgaacgcga gcagctgcgc gaagcgaaag ccgccgttgc
     8821 actggatgca tgggttgggt gtctccccac gttcgtaacc gcggacgaac gggtcgacca
     8881 ccgcacgccg aaactcctcg cggaggtcga gcgtcacgtg cgggacgccg agcgagtggc
     8941 acgtcgcacg ggcagcgaga acggcatcgg gagagcaaca ggcgcgctcg gagtcaggcc
     9001 catcgggatc gagccagagc cgcagcgtga cgccgacggc gttcggcaag gacttgagca
     9061 gtgcaacggc gctgtcgaca ccgccgctca tagcaacggc gacgcgacca ggagaacgcg
     9121 acgcccgaaa cacgggtcca atcgcattgg caagcgcgtc aactgcgagc gtctcacccc
     9181 cgacagaagc agcctcgagc agcgtcaatc caacgagcga ccgatcgagc ccggaagcat
     9241 cggcctcgac gatccgatca ccatcgaaag caagacgcac gagcgcgaag tcacagcctc
     9301 gag
//
LOCUS       DASVEU010000029         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_603922,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000029 DASVEU010000000
VERSION     DASVEU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603922"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..833)
                     /locus_tag="VF225_01165"
     CDS             complement(3..833)
                     /locus_tag="VF225_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7143892.1"
                     /translation="MSAIAELAEERIVEGVYAVARKQRLRTKGGAPYLALELVDPSGR
                     IDARVWHDVELLDARFAEGDAVRVLGRVEKFRNRLQVDVRTLEAAPEADPAEFAPALR
                     RDADELDGFLEFLAGEISHDGLAGVVTRFFSDDVLRASLRRLPASETHHSYAGGLLEH
                     TVGVATICRETAQLHPRLRSDLLLAAALLHDVGRVAELTSGPTFRQTDEGRLLGHVHL
                     GLRMVEERSGGMQPDARSELLHAIACHHDARSARTAEASVLYHANQLDAVAATRPIPD
                     "
     gene            1035..2054
                     /locus_tag="VF225_01170"
     CDS             1035..2054
                     /locus_tag="VF225_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX7143893.1"
                     /translation="MAVVKPFRAERYDESKAGPLERLVSPPYDVISPEQREAYLARSP
                     YNVVHLTLPDDEEQAGRDLVAWREEGVLARESEPGYWFLAQDYVGPDGIPRTRSGLVA
                     SLRTEPYETGAVLPHERTHRGPREGRLRLLRATRTQLEPIFLLYEGDPLQPPDRAPDL
                     QSGGDKLWRIVDAPSFRSTELLIADGHHRYETAVAYAQEGGSPYLMVVLVPTNQEGLT
                     IFPTHRMAAHVNGARGTPIDEPGDELPGVVMYRDGKYELLGGDGLDVEIVEQIGPEGV
                     TYTPQRADAVATVDRGEAEAAFLLRPTRIEDVWAVARRGETMPQKSTYFYPKLTSGLL
                     FHPLD"
     gene            2047..>2710
                     /locus_tag="VF225_01175"
     CDS             2047..>2710
                     /locus_tag="VF225_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7143894.1"
                     /translation="MTDWLTLCRAAVEDVRGVLAELPRRSDREPVVGDGMGGDETTAI
                     DAAAERVILARFEGLGDLTIVSEEVGVLGDGATHVVIDPIDGSLNAKRGIGFFALSIA
                     VASGPTMGDVDFGFVHDFGTGEEWTATRGEGARLDGTRLDGELPKERIEILAFEATRT
                     ASVAEKAAAVVDLAYRLRIMGSLALSLCHLAAGRIDAVCSLKPARAVDIAAAQLLVRE
                     RGL"
BASE COUNT          424 a          904 c          952 g          430 t
ORIGIN      
        1 ggctagtcgg gtatcgggcg cgttgcggcg accgcgtcga gctggttcgc gtgatacagc
       61 accgacgctt cggccgtgcg tgccgaccgt gcgtcgtgat gacaggcgat tgcgtgcagc
      121 agctcggagc gcgcgtcggg ctgcatcccg ccgcttcgct cctcgaccat gcgcaagccg
      181 agatggacgt gccccaggag gcggccttca tcggtctgcc ggaacgtcgg cccggaggtg
      241 agctcggcga cgcggccgac gtcgtgcagc aaggctgcag cgagcagcaa gtcggagcga
      301 agccgcggat gcagctgcgc cgtctcgcgg cagatcgttg ccacgccgac ggtgtgctcg
      361 agcaagccgc ccgcgtacga atgatgtgtt tccgaagcag gcaaccggcg cagggaggct
      421 cgcagcacgt cgtcggagaa gaaccgcgtg acgacgcccg cgaggccgtc gtgcgagatc
      481 tcgccggcga ggaattccag gaacccgtcc agctcgtcgg cgtcgcgtcg cagtgccggc
      541 gcgaactcgg ccgggtcggc ttcaggggct gcctccagcg ttcgcacgtc cacctgtagc
      601 cgattgcgga acttttcgac gcggccgagc acgcgcacgg catcgccctc cgcgaagcgc
      661 gcgtcgagca gctcgacgtc gtgccaaaca cgcgcatcga tgcggccgct cggatcgacc
      721 aactcgaggg caaggtacgg ggcgccgccc ttcgtgcgga gccgctgctt gcgcgcgacc
      781 gcatagacgc cctcgacgat ccgctcttcg gccagctctg cgattgcgct catcggccgc
      841 tctttggccg atgagcggtg gagggggcgt aaggggagac cgggaggttt cccccctctt
      901 tctcctcgac gcctgcggcg gcggtcgcaa cggcgtgggc gtcgaggcga cttctaaaga
      961 agggaacgag ccgaaggcga gtgacgctca caaagtgagt ctaggtccga acccggatgc
     1021 tagggtcggc gcaaatggcc gtggtcaagc cgttccgtgc cgagcgctac gacgagtcga
     1081 aggcgggccc gctggagcgg ctcgtgtcgc cgccgtacga cgtgatctcg cccgagcagc
     1141 gggaggcgta cctcgcgcgc agtccgtaca acgtcgtcca tttgacgctc ccggacgacg
     1201 aggagcaggc aggccgtgac ctggtcgcct ggcgcgagga gggcgtgctc gcgagggagt
     1261 cggagcccgg ctactggttc ctcgctcagg actatgtcgg cccggacggc attccgagga
     1321 cgaggtccgg cctcgtcgcc tcgctccgaa cggagccgta cgagaccggc gccgtactcc
     1381 cgcacgagcg aacgcatcgt ggccccaggg agggccggct tcggctgctc cgtgccaccc
     1441 gcacgcagct cgagccgatc ttcctcctct acgagggcga cccgctccag ccccctgacc
     1501 gcgctcccga tctccagagc ggcggcgaca agctgtggcg catcgtggat gctccgagct
     1561 tccggagcac ggagcttctg attgcggacg gccaccaccg ctacgagacc gcggttgcgt
     1621 acgcgcagga gggcggctcg ccctacttga tggttgtgct cgttccgacg aaccaggaag
     1681 gcttgacgat ttttccgacc catcgaatgg ccgcacacgt gaacggcgcg cgaggtactc
     1741 cgatcgacga gccgggggac gagttgccgg gcgtcgtcat gtaccgcgac gggaagtacg
     1801 aactgcttgg cggcgacggt ctcgacgtgg agatcgtcga gcagatcgga ccggagggcg
     1861 tgacctacac cccgcagcgc gccgacgccg ttgcaacggt cgaccgcggc gaagccgaag
     1921 cagcgtttct gcttcgcccc actcggatcg aggacgtctg ggcggtcgca cgacgcggcg
     1981 agacgatgcc gcagaagagc acctacttct atcccaagct cacgtccggc ctgctgttcc
     2041 acccgcttga ctgactggct cacgctctgc cgcgcagcgg tcgaggacgt gcgcggcgtg
     2101 ctcgccgaac tgccacgccg gtccgaccgt gagccggtcg ttggtgacgg catgggcggc
     2161 gatgagacaa cggcgatcga tgccgcggcc gagcgtgtga ttctcgcccg cttcgaaggg
     2221 ctcggcgact tgacgatcgt ctccgaggag gtcggcgttc tcggcgacgg cgccacgcat
     2281 gtcgtgatcg acccgatcga cggttcgctg aacgcgaagc gcggcatcgg cttcttcgcc
     2341 ctgtccatcg ccgtggcttc cggcccgacc atgggcgacg ttgacttcgg cttcgtccat
     2401 gacttcggca cgggggaaga gtggacggcg acgcgtggcg agggagcgag actcgacggg
     2461 acgcggctcg atggagagtt gccgaaggag aggatcgaga tcctggcctt tgaagcgaca
     2521 cgcaccgcct ccgtcgccga gaaggctgcg gcagtggtcg atctggccta ccgcttgcgg
     2581 atcatgggct cgctcgcgct ttcgctgtgc cacctggccg ccggccgcat cgacgccgtc
     2641 tgctcgctca agccggcacg cgcggttgac atcgctgcgg cccagctcct cgtccgcgag
     2701 cgcggtctcg
//
LOCUS       DASVEU010000030         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1188889,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000030 DASVEU010000000
VERSION     DASVEU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188889"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF225_01180"
     CDS             complement(<1..219)
                     /locus_tag="VF225_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7143895.1"
                     /translation="MEQARVDKWLWAARFFKTRSAATQAVLGGRAHVNGVRVKPSHEV
                     RPGDTIEVTMRSEKRTVDIVEIADRRGPA"
     gene            complement(588..905)
                     /locus_tag="VF225_01185"
     CDS             complement(588..905)
                     /locus_tag="VF225_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143896.1"
                     /translation="MGFEQYHEPANELSAEVRTFARMIASLTEEAEAIGWYEQRIALE
                     PDPQAKAIMANAQGEEFKHFGMDLEFLLRRTPDWRIALKTILFTDGDIVERGEAAEDA
                     ESR"
     gene            complement(1021..1176)
                     /locus_tag="VF225_01190"
     CDS             complement(1021..1176)
                     /locus_tag="VF225_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143897.1"
                     /translation="MLFHRAATHLGFDPDATAPAEEADFIAANVADVIVVDNETALVT
                     PTPSPPT"
     gene            1231..1923
                     /locus_tag="VF225_01195"
     CDS             1231..1923
                     /locus_tag="VF225_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7143898.1"
                     /translation="MRVALLYDIHGNLVALEAVLADVEAAGATSYLLGGDYASLGPWP
                     RQTAELLESLSAIVRIRGNVERWLREDPEAPESAQQFLKTALTAARESLGPALVERLY
                     RLPEQGELDGILVCHGSPLSDIESFAPEAEPNEERMLAGESNRTILFGHSHRQFRRAG
                     PNGTLLVNPGSVGAPLDGDPRAAWAIYENGEIGFRRTAYDVERAAAQMRTYGDWADPI
                     VYRIEHGSDRPP"
     gene            2008..2436
                     /locus_tag="VF225_01200"
     CDS             2008..2436
                     /locus_tag="VF225_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX7143899.1"
                     /translation="MSAADKSVREYATDEIVVEWDSRLCYHSHNCVRALPQVFDPDRR
                     PWVMVDAASADEVEAAVARCPSGALRARRVGVEPPKREQPLELRASAKGPLLVSGGVR
                     VVAADGTVLYEGEKAALCRCGGSANKPFCDGTHKTIDFEG"
     gene            2441..>2997
                     /locus_tag="VF225_01205"
     CDS             2441..>2997
                     /locus_tag="VF225_01205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7143900.1"
                     /translation="MLTLYQAEWCPFSSAVREVLTELGVDFVARQVEPWPEERRELLT
                     FAGTDQIPVLQAEDGRLYRGTREIFAHLRELEPWQFAAEHRRRFTDHRDARESDAQGQ
                     LVEYFHASETFEDVVGHPAQAEVVDVPEASRYELRLAERLIGLVAYRRRDGRIAFTHT
                     EVDESWEGRGFGSRLAAAALDDARR"
BASE COUNT          476 a         1029 c          991 g          501 t
ORIGIN      
        1 cgccggtccg cgcctgtctg cgatttcgac gatgtccacg gtgcgcttct cggagcgcat
       61 cgtcacctcg atggtgtctc ccgggcgaac ctcatgcgac ggcttcacac gcacaccgtt
      121 cacatgggcg cggccgccga ggacggcctg cgtcgccgca ctacgagtct tgaagaagcg
      181 cgccgcccac aaccacttgt cgactctcgc ctgttccatc gctactgcct gactgtcgga
      241 gaccgctccg accgtggcga aaggtcgccg acaaggctcg agaagggacg ccagttgtcg
      301 tagtcccagg tcgatccgcc acacatcggg cagcgttccg gcgccgaccg ccggctcgct
      361 ccgtaggaac acccggtgca gcgaaagcga aacagcggtc cttggtcgcc cgtcggtctt
      421 cccacgtcga actcctcaac acccattcgg acccctttca aacgggccgc gaggtctcgg
      481 cggcggagtt aggcggcggc agaatagccg aaatcggccc tcgttcaaaa acctgccgcg
      541 tcagccgcga ctgcgcccgt cttcacgaaa atcggtctca ctcgcagcta gcggctctcg
      601 gcgtcttctg ccgcctcgcc gcgttcgacg atgtcgccgt cggtgaagag gattgtcttc
      661 agcgcgatcc gccagtccgg cgtgcgtcgc agcaggaact cgaggtccat gccgaagtgc
      721 ttgaactcct ccccctgcgc gttggccatg atcgccttcg cctgcggatc gggctcgagc
      781 gcgattcgct gctcatacca gccgatcgct tcggcctcct cggtcagcga cgcaatcatg
      841 cgtgcgaagg ttcgaacctc ggcgctcaac tcgttcgccg gctcgtgata ctgctcgaat
      901 cccatttctg ctcctctctc ggctgggtgg cgcggcacgg gttgagaagc gcccgtgccg
      961 gagcatgtca ctctcgcgcg agcttcgcgt tccgctcgag tgccctcttg atcgagtgct
     1021 tcacgtcggc ggactcggtg tcggagtcac gagcgcggtc tcgttgtcga ccacgatcac
     1081 gtcggccacg ttcgccgcga tgaaatcggc ctcttccgca ggagctgtcg cgtcgggatc
     1141 aaagccaagg tgggtggcag cgcggtgaaa caacatgccg ctcgatccta gacctcactc
     1201 gccccaccga cctggtgtca caatcggccg gtgagggttg cgctgctcta cgacatccac
     1261 ggcaacctcg tggctctcga agccgtgctt gcggacgtcg aggcggcagg agcgacgtcc
     1321 tacctgctcg gcggcgacta cgcctcgctc ggtccgtggc cgcgccagac ggccgagctg
     1381 ctcgagagcc tgtcggcgat cgtccgcatc cgcggcaacg tcgaacggtg gctgcgcgaa
     1441 gatccggaag ccccggagag cgcgcagcaa ttcctcaaaa cggcgttgac cgcggcgcgc
     1501 gagtcgctcg gccctgctct ggtcgaacgg ctgtaccggc ttcctgagca aggggaactg
     1561 gacggtatcc tcgtctgcca cgggtccccg ctctccgaca tcgagagctt cgcacccgaa
     1621 gccgaaccga acgaggaacg gatgctcgcc ggcgagtcga accgaacgat tctgttcggc
     1681 cacagccacc ggcagtttcg tcgagcgggg ccgaacggaa cgctgctggt gaaccccggc
     1741 agtgtgggtg cgccgctcga cggcgaccca cgcgcagcct gggcgatcta tgagaacgga
     1801 gagatagggt ttcgcagaac cgcgtacgac gtcgagcgag cggccgcgca gatgcgcacg
     1861 tacggcgact gggccgaccc gatcgtgtac cggatcgagc acggatccga ccggccgccg
     1921 taaaccccgg cggaggtggc gactctcgtg cctctcctag ccttaccgcg cgcagctaac
     1981 gtcaccggtg cgacctaggg aggcacgatg agcgcggctg acaagtccgt tagggaatac
     2041 gcgaccgacg agatcgtggt cgagtgggat tctcgcctct gctaccactc gcacaactgc
     2101 gtccgcgcgc tgccgcaggt cttcgatccc gaccggcggc cgtgggtcat ggtcgacgcg
     2161 gcgtcggcag acgaggtcga ggcggccgtc gcccggtgtc cgtccggcgc gctgcgagcg
     2221 cgccgcgtcg gcgtcgagcc cccgaagcgg gagcagccgc tcgagctgcg cgcatctgcg
     2281 aaaggcccgc tgctcgtgtc gggcggtgtg cgggtcgtgg ccgcggacgg caccgtcctg
     2341 tacgagggcg agaaggccgc gctgtgccgc tgtggcggct cggccaacaa gccgttctgc
     2401 gacgggacgc acaaaacgat cgacttcgag ggctgacgcc gtgctgacgc tctaccaggc
     2461 ggagtggtgt ccgttctcct cggctgtgcg cgaggtgctg accgagctcg gagtcgattt
     2521 cgtcgcccgt caggtcgagc cttggccgga ggagcgtcgc gagctcctga ccttcgccgg
     2581 taccgaccag atccccgtgc tccaggccga agacggccgg ctttaccgtg gcacgcgaga
     2641 aatcttcgcc cacctgcgcg agctggagcc gtggcaattc gctgcggagc accgccgccg
     2701 gttcaccgac catcgcgacg cccgcgagtc ggatgcccaa gggcagctgg tcgagtactt
     2761 ccacgcgagt gaaacgttcg aggatgtcgt cggccacccg gcgcaggccg aggtcgtcga
     2821 cgtgcccgaa gcgagccggt acgagctgcg cctcgccgag cgcctcatcg gcctcgtggc
     2881 ttaccgacgc cgggacggcc ggatcgcctt tacgcacacc gaggtggacg agtcgtggga
     2941 ggggcgcggc ttcggcagcc gtctcgccgc tgcggcgttg gacgacgcgc gccgcca
//
LOCUS       DASVEU010000031         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1189793,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000031 DASVEU010000000
VERSION     DASVEU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189793"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..585)
                     /locus_tag="VF225_01210"
     CDS             complement(55..585)
                     /locus_tag="VF225_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7143901.1"
                     /translation="MLDLGCNIGLTVAHFEVLWPNAEIIGVDLDSENCVVARRNCRRA
                     RILNLAVSATSGRQTYSGDEAWGFRLDPLGDRAVEARTLDELTDLLGGRADFVKMDIE
                     GAEWEVLKAPGEWPERIGSLLIEIHGTEGRRQAGIDEMMGYLRDKGFKCGKHEAHWSA
                     VWAKRESRTGSLDTRT"
     gene            complement(714..1700)
                     /locus_tag="VF225_01215"
     CDS             complement(714..1700)
                     /locus_tag="VF225_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7143902.1"
                     /translation="MKIVMTLLVRNEEDILDAHLSFHRAAGVDLVLAIDNDSDDSTSD
                     ILDSHARDGFVDVRRETGDLQQAEWVTKLARRAATEFGADWVINSDADEFYWPRGGTL
                     KDVFSAVPGRYGGVRGMLRNFAPRPESDESFAEQMIVRVCNPGAEDNSPYSPRFKAMH
                     RGAPDVTVLPGSNRVLGRGLDPLPGWYPIDVLHFPIRSLEHFTVKYLRWWALLGTRFS
                     EAVHEAHSRGALREFYESYMVDDEALAKGVADGTLAVDTRLRDALRSLRAEPSRALAF
                     SDAAVDEAYLAELALLENGEPHAHVQRKAEDLEARLAVIERSRFKALMRRFA"
     gene            1739..>2092
                     /locus_tag="VF225_01220"
     CDS             1739..>2092
                     /locus_tag="VF225_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143903.1"
                     /translation="MPNVRDAAKTLLAVVLCTMARLSGARAGIALVYHRVGGEGGDSR
                     LEILAAVSGESFRRQLRHLARHYRVVRGSELLDAVHARRRGQRFPAAITFDDDLTGHV
                     RDALPALQEAGLSATF"
BASE COUNT          346 a          710 c          666 g          370 t
ORIGIN      
        1 cgaaggaagg ttctcgtcgc tgctcggctc cgaaggggat tgtcggcccc gcggctaggt
       61 tctcgtgtct agcgaacctg tacggctctc cctcttcgcc cagaccgccg accagtgcgc
      121 ctcatgcttc ccacacttga agcccttatc tcgaaggtac cccatcattt cgtcgattcc
      181 cgcctgacgc cgcccttcgg tgccgtggat ctcgatcagc agactgccga tgcgctcggg
      241 ccattcgccc ggcgccttca gaacttccca ctcggccccc tcgatatcca tcttgacgaa
      301 atcagcgcgg cctccgagga ggtcggtcag ttcgtcgagc gtacgagcct cgacggcccg
      361 atctcccaaa gggtctaggc gaaaacccca cgcttcgtcg ccggagtacg tctgcctgcc
      421 cgaggtcgcg gaaacggcga ggttgagaat ccgagcccgc cgacaattac ggcgggccac
      481 cacacagttc tcggagtcga gatcgacgcc gatgatttcg gcgttcggcc agagcacctc
      541 aaagtgagca accgtgaggc cgatgttgca gccgaggtcg agcacggtcg ccgggcggat
      601 ctcttccggc ggaacgtgga actgcttgac gaacgtctcg tggaagacct gatcatccgt
      661 cgtgcctggc cgaagttgta cctcgcgacc gagcgcttgc acgtagcgcg tgatcatgcg
      721 aagcgtcgca tgagagcctt gaaccggctc cgctcgatca cggcgagccg ggcctcgaga
      781 tcctcggcct tgcgctgcac gtgggcatga ggctcgccgt tctcgaggag cgccagctcg
      841 gccagatacg cctcgtccac ggcggcgtcg gagaacgcga gcgctctcga cggttcggcg
      901 cgtagcgacc gcaatgcgtc gcgcaaccga gtgtcgaccg cgagcgtgcc gtccgcgact
      961 cccttcgcga gcgcttcgtc gtcgaccatg tacgactcgt agaactcccg cagcgcccca
     1021 cgagagtggg cttcgtgcac cgcttcgctg aagcgcgtgc ccagcagagc ccaccagcga
     1081 aggtacttca ccgtgaagtg ctccagcgag cgaatgggga agtggaggac gtcgattgga
     1141 taccagcccg gcaatgggtc gagcccgcgg ccgagcactc gattactgcc cggcaagacc
     1201 gtcacgtcgg gagcaccacg gtgcatcgcc ttgaagcgcg gactgtaagg gctgttgtct
     1261 tcggcgcctg gattgcagac gcgaacgatc atctgctcgg cgaaagactc gtcgctctct
     1321 ggtcggggcg cgaagttgcg caacatgccc cgcacgcctc cgtagcgccc cggcaccgcc
     1381 gaaaaaacgt ccttgagcgt ccccccgcgc ggccagtaga actcgtcggc gtcgctgttg
     1441 atcacccagt ccgcgccgaa ctccgtcgcg gcacggcgcg ccagtttcgt cacccactcg
     1501 gcttgctgca ggtcgccggt ctctcgcctg acgtcgacaa acccgtcccg tgcgtgggaa
     1561 tcgaggatgt cggaggtcga atcgtcggaa tcgttgtcga tcgcgaggac gagatcgacg
     1621 ccggctgcgc gatggaagga gaggtgcgcg tcaaggatgt cttcctcgtt tctcaccagc
     1681 agcgtcatga cgatcttcat gcagccgtct ttctagccga agcggtagct tgagcggctt
     1741 gcctaacgtc cgggacgccg ccaagacgct gcttgccgtg gtgctctgca cgatggcgcg
     1801 actttcgggc gcgcgcgccg ggatcgcgct cgtctaccac cgtgttggcg gcgagggtgg
     1861 cgactcgcga ctggagatcc tggccgcggt ctccggcgaa tcgttccggc ggcagctgcg
     1921 ccacctggct cgccactacc gggtcgtccg agggagcgag ctgctcgatg ccgtgcacgc
     1981 gcggcgccgc gggcagcgct tccctgcggc gatcacgttc gacgacgacc tcacagggca
     2041 cgtgcgcgac gcgctccccg ccctccagga ggccggcctg agcgcaacgt tc
//
LOCUS       DASVEU010000032         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_802287,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000032 DASVEU010000000
VERSION     DASVEU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802287"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF225_01225"
     CDS             <1..837
                     /locus_tag="VF225_01225"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HEX7143904.1"
                     /translation="RVVIDAANGMGGVMLAPILERLPIDAVPYFFEPDATFPNHEPNP
                     LLPENRQFIIEKVHADRADLGVAFDGDADRCFFVDDTGEFVPGDFVTALFAESILEKE
                     PGAKILYDVRASWAVPQTIERAGGVPLVNRVGHAFFKHRMREEGAVFGGEVSGHYYFQ
                     NFSRADSGVIPFLLMLELISKRGRKLSELLAPYRERYFITGELNTPVDDVALKLQELK
                     ERFGPEGKISHLDGLSVDAENWHMNVRPSNTEPLLRLNLEALSPDLMERKRDEVLEVI
                     RS"
     gene            834..1274
                     /locus_tag="VF225_01230"
     CDS             834..1274
                     /locus_tag="VF225_01230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7143905.1"
                     /translation="MKEKPPFKFSAQTRVGFSDTDAQGIVYYGRYLPYFDLARVEYHR
                     NLGLLGMDIGDEGQEFVMRALGIEYLAPAVFDDLIEVYVRMARIGRTSVTFELAAYRA
                     SDDELMVTATQTLVLVDLDERKAVSIPETYKEAIRAFEGEALET"
     gene            1271..1621
                     /locus_tag="VF225_01235"
     CDS             1271..1621
                     /locus_tag="VF225_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143906.1"
                     /translation="MSAHDGALEAIDEIVSHGGEADEVLREVVAVLHRDYSWVGISFV
                     EAGELVLGPAQGERTAEPTAIPISYENNVVAELGVVAPGLSPEDRALFERVAELLAPY
                     CLVGWDTGGEAWTP"
     gene            complement(2129..>2862)
                     /locus_tag="VF225_01240"
     CDS             complement(2129..>2862)
                     /locus_tag="VF225_01240"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX7143907.1"
                     /translation="TREDQEQKGRDSLEQSLKAAKLPYRKLAGSAVLAEVIRDMNFKK
                     AEDFYLALGSGKIGPQQVVNKVLQRLKTEQVAEEEAVPLKAPRAKHAVDSDNYGINVH
                     GVDEVLVRLAKCCTPVPGDEIVGYISLGKGITIHRGDCPNVKALARQPERFTPVSWDG
                     GATQSFRVQIAVESWDRPRLLEDVARTFAEHGANIVSYGGSVEDQMARNWYVAEVGDV
                     KELRGLLTALRNIEAVFDAYRVTPS"
BASE COUNT          489 a          951 c          925 g          497 t
ORIGIN      
        1 cgcgtcgtca tcgacgctgc gaacggcatg ggcggagtca tgctggcgcc aattctcgag
       61 cgcctgccga tcgatgccgt gccgtacttc ttcgagccgg acgcgacatt cccgaaccac
      121 gaaccgaacc cactgcttcc cgaaaatcgt cagttcatca tcgagaaggt gcacgccgac
      181 cgggccgatc tcggcgtcgc cttcgacgga gacgccgacc gctgtttctt cgtggacgac
      241 accggcgagt tcgttcccgg tgacttcgtg acagcacttt tcgcagaatc gatcctcgag
      301 aaggaaccag gcgcaaagat cctctacgac gtgcgcgcaa gctgggctgt gccgcagacg
      361 atcgaacgcg caggcggcgt tccgctcgtc aatcgcgtcg ggcatgcgtt cttcaagcac
      421 cgcatgcgcg aggaaggcgc cgtcttcggg ggggaggtct ccggccatta ctacttccag
      481 aacttctcgc gggccgactc cggcgtgatt cccttcctgc tcatgctcga gctcatttcc
      541 aagcgagggc ggaagctctc ggagctgctc gctccgtacc gcgagcgcta cttcatcacg
      601 ggcgagctca acactcctgt cgacgacgtc gcgctgaagc tgcaggagct gaaggagcgc
      661 ttcgggcccg agggcaagat ctcccacctc gacggcctct cggtggacgc agagaactgg
      721 cacatgaacg tccggccgtc caacaccgag ccgttgctgc gcctgaacct cgaagcgctc
      781 tcgcccgatc tgatggaacg caagcgcgac gaggtgctcg aggtcattcg ttcttgaagg
      841 agaaaccccc gttcaagttc agcgcgcaga cgcgggttgg cttctcggac acggacgcgc
      901 agggaatcgt ctactacggc cgctacctcc cctacttcga cctcgcgcgc gtcgagtacc
      961 accgcaacct cggcctgctc gggatggaca tcggcgacga aggccaggag ttcgtcatgc
     1021 gcgctcttgg gatcgagtac ctggcgccgg ccgtcttcga cgacctgatc gaggtctatg
     1081 tgcggatggc gaggatcggc cgcacgagtg tcacgttcga gctggcggcg tatcgggcga
     1141 gcgacgacga gctgatggtg acggctaccc agactctcgt gctcgtcgac ctcgacgagc
     1201 gcaaggccgt gtcgatcccg gagacctata aggaggccat tcgtgccttc gagggcgagg
     1261 cactcgagac gtgagcgccc acgacggcgc gctcgaggcg atcgacgaga tcgtcagcca
     1321 cggcggggag gcggatgaag ttctgcgcga ggtagtcgcc gtcctccacc gtgactattc
     1381 atgggtcgga atctcgttcg tcgaggcagg cgagctagtc ctcggaccgg cgcaggggga
     1441 gcggacagcc gagccgacag cgattccgat ctcctacgag aacaacgtcg tcgccgagct
     1501 cggcgtcgtc gcacccggcc tgtcgcctga agaccgcgcg ctcttcgagc gagtcgcaga
     1561 gttgctcgca ccgtattgcc tcgtcggctg ggacaccggc ggcgaagcct ggacgcccta
     1621 gagccgcgcg aaccatcgcg accaccaagg ccggaagcta agaccaggcg aagccgggct
     1681 tagctgcagg cgaaccgatg cggaagggaa ccaccgccga gcggcaacac caaaggagtg
     1741 cgcgggctcg gcccgcgcac gttccccgcg aagcggcgta cccaagatcc gggcgaaagc
     1801 ggagctttcg cccggggcga agcgcgaagg cgcgccggcc gtccggcggc cggcgcagac
     1861 gcctcacctg agcgaagcga atctagtgag ggccgcgcgc ccgcccgttg tcctcgaaac
     1921 ggtcgggcgc cgcagctttg gtgccctctc cccctagcac tctttgtagt cagattcgcg
     1981 gcgcgcgcca tccctgaaac gaggtacctc gctcgttcga gggacgatcc cttgaacgag
     2041 gaagtggccg ttgacgcgtc gtgttacaac gcgggcaccg cagactcggc ccgcgtcgtt
     2101 ggagtgacat tccctcgaag gagggacgtc agctcggcgt cacgcgatat gcgtcgaaga
     2161 cggcttcgat gttccggagc gccgtcagca agccgcggag ctccttgacg tcgccgacct
     2221 ccgcgacgta ccagttgcgg gccatctggt cttcgacgct gccgccgtac gagacgatgt
     2281 tggctccgtg ctcggcgaac gtccgcgcca catcctccag gaggcgcggc cggtcccacg
     2341 attcgacggc gatctggacg cggaagctct gcgttgcgcc gccgtcccag ctcacgggtg
     2401 tgaagcgctc gggctggcgg gcaagcgcct tcacgttcgg acagtcgccc ctgtggatcg
     2461 tgatgccttt gccgagcgag atgtaaccga cgatctcgtc tcccggcacc ggcgtgcagc
     2521 actttgcgag ccgcacgagc acctcgtcga cgccgtggac gttgatgccg tagttgtcgc
     2581 tgtcgaccgc atgcttcgcg cgcggcgcct tgagcgggac tgcttcctcc tcggcgacct
     2641 gctccgtctt caagcgctgc aggaccttgt tgacgacctg ctgcgggccg atcttgcccg
     2701 agccgagtgc aaggtagaag tcctcggcct tcttgaagtt catgtcgcgg atgacctctg
     2761 cgagcacggc cgaaccggcg agctttcggt acggcagctt cgccgccttc aacgactgct
     2821 cgagcgagtc gcggcccttc tgctcctggt cctcgcgcgt ct
//
LOCUS       DASVEU010000033         7600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1580447,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000033 DASVEU010000000
VERSION     DASVEU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580447"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2187)
                     /locus_tag="VF225_01245"
     CDS             complement(64..2187)
                     /locus_tag="VF225_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7143908.1"
                     /translation="MSAAETVRLTIDGVGVEVPKGTGLVEAALAAGIEIPVFCYEPRL
                     GPAVGACRMCLCQIEPGPPKPQAACTLTAQEGMVVRTALTSQVAAEAQNATLEFILVN
                     HPLDCPVCDTGGECPLQDLTFRYGPGNTRMTFPKRTFEKPIPVSPLIALDRERCILCY
                     RCTRFSEGVAEDGQLVAVDRGAGTMIATFGDEPYRAPFTGNVIELCPVGALTSTTYRF
                     EARPWEIQDVPTVCGMCPVGCNVNATTREGKVKRILSRNHPEVDDGWLCDKGRFSYAH
                     LHAEDRLRDPILRVRRRGFEEVSWERALDEGERLLREADGRIVTALSGSETVEQAYAL
                     GKLLRRGLGAHTAFFAEESSDALDAFRVPLSAIRDADVVTVVGDGPVVERAPIVDLWI
                     KEARRRGARVLYELDEEAVRGAKRAILIWCGHGGHGGATVAALAQRLGASGAFYIPET
                     ANGRGVCDAWSCSDDAESPSPDPIGLLIVSGDEAAANPDVRALAEKAERVLVISMFQG
                     LAAGWADLVLPGTSYLERDGTYVNLEGRVQRLRRTVIPPVPDELAWISKLAERFGVEV
                     SPYPSLVFEEVAAIAFGGIPFGGIGERAPLPTAIGATAPIDVPAEPSDNGGLRLVRYR
                     PLFSGPAVERVRELDFQRPQPEAELSEQDGRARGIRNGEEITIRSNGTSVTLRARLSR
                     ELRPGVVRVPETFAGDLKPIVEISK"
     gene            complement(2240..3562)
                     /gene="nuoF"
                     /locus_tag="VF225_01250"
     CDS             complement(2240..3562)
                     /gene="nuoF"
                     /locus_tag="VF225_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7143909.1"
                     /translation="MATEPRLIVMAGTEESDLTRLAEYEAIGGYRALQKARGLTPQAV
                     IDEVIASELRGRGGAFFPTGRKWAFVPKPDQNPNPHYVVVNADESEPGSFKDNEIMAR
                     VPHRFFEGCLIAAHAIESKNVFVYIRGEYTGPYEILLAALKELEARPDLRGDVTIVLH
                     RGAGAYICGEETALLESLEGKRGQPRTKPPFPAIQGLYASPTVVNNVETLATLPPIIE
                     MGGAEYAKLGVPDSRGTRLVSISGHVERPGNYEIENGTSLRDLIYGLGGGIPGGRALK
                     AVIPGGSSTVILKGDEIDVGYDFNSLLKLNTAMGSAGVVALDDSCCMVQLGIRISEFY
                     EHESCGKCTPCREGTRWMTQILRRLESGEAVQSELDLLLDVCDRINGKCLCPLGETAA
                     VAVASYVEKFRAEFIAHVDQGGCPFGESSPLHDVLAPVAVHSPATVPA"
     gene            complement(3552..4019)
                     /locus_tag="VF225_01255"
     CDS             complement(3552..4019)
                     /locus_tag="VF225_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7143910.1"
                     /translation="MSTYYDEVQEIKARYPDWRSAALPALRLAQEQHGYLSPEALREC
                     AEALGTTPAFCEAVASFYDMLHLAPIGRHMVEVCTNVSCALVGAQRVLENFEQELGIR
                     AGETTEDGEVTLRPIECLGGCGWATVVSVDHHYRTHVKPEDVPAIVQELRNGD"
     gene            complement(4016..5278)
                     /gene="nuoD"
                     /locus_tag="VF225_01260"
     CDS             complement(4016..5278)
                     /gene="nuoD"
                     /locus_tag="VF225_01260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX7143911.1"
                     /translation="MAVAPERPRIYEGTRIPSPTPSVLEVREDLQTSEDVLTINFGPN
                     HPSTHGVLRLVVDLHGEEVVGLAAVIGYLHTGFEKNMEQKSWWKAITYAPRIDYLSFQ
                     NNELVFVLAVEKLLEIEVPARATWMRMLLAELNRIHSHLVWLGTAALELGAISMFWYC
                     FRERDRILDLFELVAGSRMHTRYFQVGGLAEDVPAGFWDECAKFVEVMPKAVDDYEGL
                     VDRNEIWLERTQGIGLLSADDAIALGQSGPVLRGSGVDWDLRRAEPYLAYDQVDFQVP
                     VYQEGDVYARYRVRMEEMRESVKIVEQAMRRMPGGPWIADDRKVVLPPREELHTSMES
                     LIHHFKIVTEGFRVPEGEVYVTSESPRGELGCYVVSDGGSKPWRVKFRAPSFVALEAT
                     ATCMRQALVADMIAIVGSLDTVMGEVDR"
     gene            complement(5279..5797)
                     /locus_tag="VF225_01265"
     CDS             complement(5279..5797)
                     /locus_tag="VF225_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7143912.1"
                     /translation="MEVHEAHGETTIVVDTPRLEDAALHLRDERGFNFLSDVAATDYL
                     GWGAKGVSGYIGTPRGRDLNEPATQGHQRLPRQKPHRFSVSYHLLGMRAGAPRVRLQA
                     WVEDGEHVPSLVGVWPTADWHEREAFDLMGIRFDGHPNQRRILMDDDWEGHPLRKDYP
                     IGGEPVRFSGEE"
     gene            complement(5821..6405)
                     /locus_tag="VF225_01270"
     CDS             complement(5821..6405)
                     /locus_tag="VF225_01270"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7143913.1"
                     /translation="MAIEDGKKLRDHGLQSERLLWPTKGPGVFEQEVAAMEQAVGLTT
                     LEKAVAWAQSKSMWPDTFGLACCAIEMMSIVSSRYDIARFGMEAFRSSPRQADLLIVS
                     GRVVHKMAAPLRQVYDQMLEPKWVIAMGACASSGGMFNNYTTLQGVDKIVAVDVHVPG
                     CPPRPEALMEGIVRLHEKVMAGIPPAYEIRGVAS"
     gene            complement(6396..6818)
                     /gene="ndhC"
                     /locus_tag="VF225_01275"
     CDS             complement(6396..6818)
                     /gene="ndhC"
                     /locus_tag="VF225_01275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7143914.1"
                     /translation="MLRGVGGEPVRFIVAQGEGRVLDNWLPFLIYAIFALIIPASMMW
                     FSFVFATRPHKHTRWRELPFESGVSTGAPRVQRFTVSFYLTAMLFIVFDIEIVFLYPL
                     AVILHQLAWFGFLEFLFFIAILVVAYVYVWRKGALDWQ"
     gene            6914..7318
                     /gene="erpA"
                     /locus_tag="VF225_01280"
     CDS             6914..7318
                     /gene="erpA"
                     /locus_tag="VF225_01280"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX7143915.1"
                     /translation="MELQERVVPLMTVTESAAVKIKQLIAEEADVSVLRVAIQGGGCS
                     GFQYGLGFDRGAADGDLEFEMHGVQVVVDPFSAPYLKGAEIDYVDSIQAAGFAINNPN
                     ASSSCGCGHSFQVEEGAEAEDAVGGCGSGCSA"
BASE COUNT         1256 a         2608 c         2516 g         1220 t
ORIGIN      
        1 acgcaaccat gatcaggttc acgatcactc cggccttgat cagggagatc caccacggct
       61 ccctcacttc gagatctcca cgatcggctt gagatcgccc gcgaaggttt ccgggacgcg
      121 cacgacaccc ggccggagct cgcggctcag gcgtgcgcga agcgtcacgg aagtgccgtt
      181 cgaccggatc gtgatctcct cgccgttcct gattccccgc gcgcgtccat cctgctccga
      241 cagttccgcc tcgggctgcg gccgctggaa gtcgagctcg cgcacgcgtt cgacggccgg
      301 cccggagaag agcgggcggt agcggacgag acgcaggccg ccgttgtcgc tcggttctgc
      361 gggaacgtcg atgggagctg tcgcgccgat ggccgtgggc agcggcgctc gctcgccgat
      421 gccgccgaag gggatgccgc cgaaggcgat cgccgcgacc tcttcgaaca cgagggatgg
      481 ataaggagag acttcgacgc cgaagcgctc tgcgagcttg gagatccagg ccagttcgtc
      541 gggcacgggc gggatgaccg tccgtcgcag ccgctggaca cgcccttcca ggtttacgta
      601 cgtcccgtcg cgctcgaggt agctcgttcc cggcaggacg agatcggccc agccggcggc
      661 gagcccctgg aacatcgaga tcacgagcac gcgctcggcc ttctccgcga gagcacggac
      721 gtccgggttg gctgcggcct cgtcgccgga cacgatcagc aggccgatcg ggtccgggct
      781 cggcgactcg gcatcgtcgg agcacgacca cgcgtcacag acgccgcgtc cgttcgccgt
      841 ctccgggatg tagaacgcgc cgcttgcgcc gagccgctgc gcgagggccg caaccgtcgc
      901 gccaccgtgg ccaccatggc cgcaccagat gaggatcgcg cgcttggcgc cgcgaaccgc
      961 ttcctcgtcc agctcgtaca gcacgcgtgc accgcggcgg cgcgcttcct tgatccagag
     1021 atcgacgatc ggcgcacgct cgacgaccgg cccgtcgccg accaccgtca cgacgtcggc
     1081 gtcgcggatc gcggagagcg ggacgcggaa tgcgtccaga gcatcggacg actcttcggc
     1141 gaagaaggcc gtgtgcgcgc cgagcccccg gcgcagcagc ttgccgagcg catacgcctg
     1201 ctcgaccgtc tcggagcccg agagcgccgt gacgatgcgt ccgtcagcct cgcggagcag
     1261 ccgctcgccc tcgtcgagcg cgcgctccca ggagacctct tcgaagccgc ggcgtcgcac
     1321 gcggaggatc ggatcgcgca ggcggtcctc ggcgtggaga tgcgcgtagg agaaacgacc
     1381 cttgtcgcag agccaaccgt cgtccacctc tggatggttc cgcgacagga tccgcttgac
     1441 cttgccctcg cgagtcgtcg cgttgacgtt gcaaccgaca ggacacatcc cgcaaacggt
     1501 cgggacgtcc tggatctccc acggccgagc ctcgaagcga taggtggtgg acgtcagcgc
     1561 gccgaccggg cagagctcga tcacgttgcc ggtgaagggc gcacggtagg gctcgtctcc
     1621 gaaggtggca atcatcgttc cggcgccgcg gtcgacggcg acgagctggc cgtcctccgc
     1681 caccccttcg ctgaagcggg tgcagcggta acagaggatg cagcgttcgc ggtcgagtgc
     1741 gatcagcggg gagaccggga tcggcttctc gaacgtgcgc ttcgggaacg tcatgcgcgt
     1801 gttcccgggc ccgtagcgga acgtcaggtc ctgtagcggg cattcgccgc ccgtgtcgca
     1861 gacggggcag tcgagcggat ggttgacgag gatgaactcg agggtggcgt tctgggcctc
     1921 ggccgcaacc tgtgacgtca gtgccgttcg caccaccatc ccctcctggg ccgtcagcgt
     1981 gcaggcggcc tgcggcttcg gcgggccggg ctcgatctgg cagagacaca tgcggcaggc
     2041 gccgacggcc ggcccgagtc gcggctcgta acagaagacg ggaatctcga tcccggcggc
     2101 cagcgcggcc tcgacgaggc cggtcccctt cgggacctcg acaccgacgc cgtcgatcgt
     2161 gagcctgacg gtctcggctg cgctcatcgt gacgtgcctc gaaaggcaag ggtctgaccc
     2221 cagctgttcg ggacgtgact catgcgggca ccgttgcggg cgaatgaacc gcgaccggag
     2281 cgagcacgtc gtgcagcggg gacgattcgc cgaacggaca gccgccctga tcgacgtggg
     2341 cgatgaactc ggctcggaac ttctcgacgt agctcgcgac cgcgaccgcg gccgtctctc
     2401 cgagcgggca cagacacttg ccgttgatgc ggtcgcacac gtcgagcaga aggtccagct
     2461 ccgattgcac cgcctcgccg gactcgagcc ggcgcaggat ctgcgtcatc caccgcgttc
     2521 cctcgcggca cggggtgcat ttaccgcacg actcgtgctc gtagaactcc gagatgcgga
     2581 tcccgagctg caccatgcag cagctgtcgt cgagcgcgac cacgccggcc gatcccattg
     2641 ccgtgttcag cttgagcagc gagttgaagt cgtaaccgac gtcgatctcg tcgcccttca
     2701 gaatcaccgt cgacgagccg ccggggatca cggccttgag cgcgcgcccg ccgggaatgc
     2761 cgccgccgag cccgtagatc aagtcgcgca ggctggtgcc gttctcgatc tcgtagttgc
     2821 cgggccgttc gacgtgaccg gagatcgaca cgagccgagt tccgcgcgaa tccggcacgc
     2881 cgagcttcgc gtactccgcg ccacccatct cgatgatcgg cggcagcgtc gccagggtct
     2941 cgacgttgtt cacgaccgtc ggtgatgcgt acagcccctg tatggccgga aacggaggtt
     3001 tcgtgcgcgg ctgcccgcgc ttgccctcga gggactcgag cagcgccgtc tcctcgccgc
     3061 agatgtacgc gcccgccccg cggtgcagca cgatcgtcac gtcaccgcgc aagtcgggtc
     3121 gtgcttcgag ctccttcagc gccgcgagca ggatctcgta cggcccggtg tactccccgc
     3181 ggatgtagac gaagacattt ttcgattcga tcgcgtgcgc ggcgatcagg cagccctcga
     3241 agaaccggtg cgggacgcgc gccatgatct cgttgtcctt gaagcttccc ggctccgact
     3301 cgtcggcgtt gacgaccacg taatgcggat tcgggttctg gtccggcttc gggacgaacg
     3361 cccacttccg cccggtggga aagaacgcgc cgccacggcc gcgcagctcc gatgcgatca
     3421 cctcgtcgat aacggcctgc ggcgtcaggc cgcgcgcctt ctggagggcg cggtagccgc
     3481 cgatcgcctc gtactcggcg agtctcgtca gatcgctctc ctcggtccct gccatgacga
     3541 tcaagcgcgg ctcagtcgcc attgcgcagc tcctgcacga tcgcgggcac gtcttcgggc
     3601 ttcacgtgag tgcggtagtg atggtcgacc gagacgaccg tcgcccagcc gcagccaccg
     3661 aggcattcga tcggccgcag cgtgacctcg ccgtcttcgg tggtctcgcc tgcgcggatg
     3721 ccgagctcct gctcgaagtt ctcgagcacc cgctgagcgc cgacgagcgc gcacgagacg
     3781 ttcgtgcaca cctcgaccat gtgccggccg atcggcgcga ggtgaagcat gtcgtagaag
     3841 gacgcgaccg cctcacagaa cgcgggcgtc gtgccgagcg cctctgcgca ctcacgcagc
     3901 gcctcaggcg agagatagcc gtgctgctcc tgcgcgagcc gcagcgccgg cagggcggcc
     3961 gagcgccagt cgggataccg cgccttgatc tcctgcactt catcgtaata ggtgctcatc
     4021 tgtccacctc gcccatgaca gtgtccagcg agccgacgat cgcaatcatg tcggcgacga
     4081 gcgcctgccg catgcacgtc gcggtcgctt ccagcgcgac gaacgacggg gcgcggaact
     4141 tcacgcgcca cggcttcgac ccgccgtccg agacgacgta gcagccgagc tcgccgcgcg
     4201 gggactcgct cgtcacatag acctcgccct ccggcacccg gaagccctcg gtgacgatct
     4261 tgaagtggtg gatgagcgac tccatggatg tgtggagctc ctcgcgcggc ggcagcacga
     4321 ccttgcggtc gtcggcgatc cacggcccgc ccggcatccg ccgcatcgcc tgctcgacga
     4381 tcttcacgga ctcgcgcatc tcctccatgc gcacgcgata ccgcgcgtag acgtcaccct
     4441 cctggtagac gggcacctgg aagtcgacct ggtcgtaggc gaggtacggc tccgcgcggc
     4501 ggagatccca gtcgacgccg gagccgcgca gaaccgggcc ggactgcccg agcgcgatcg
     4561 catcgtccgc cgacagcagg ccgatcccct gggtgcgctc cagccagatc tcgttgcgat
     4621 cgacgagccc ctcgtagtcg tcgaccgcct tcggcatcac ctcgacgaac ttcgcgcact
     4681 cgtcccagaa gccggccgga acatcctcgg ccagtccgcc gacctggaag tagcgcgtgt
     4741 gcatccgcga accggcgacc agctcgaaga ggtcgaggat gcgatcgcgc tcgcggaagc
     4801 agtaccaaaa catcgagatc gctccgagct cgagcgcggc cgtcccgagc cagacgaggt
     4861 gcgagtggat cctgttcagc tccgcgagca acatgcgcat ccaggtcgcg cgcgccggca
     4921 cctcgatctc gagcagcttc tcgactgcca ggacgaatac gagctcgttg ttctggaagg
     4981 acaggtagtc gatgcgcggc gcgtacgtga tcgccttcca ccagctcttc tgctccatgt
     5041 tcttctcgaa gccggtgtgc aggtagccga tcactgcggc gagcccgacg acctcctccc
     5101 cgtgcaggtc gacgacgagc cgcaggacgc cgtgcgtcga gggatggttc gggccgaagt
     5161 tgatggtgag gacgtcctcc gaggtctgca ggtcctcacg cacttcgagc acgctcgggg
     5221 tcggcgaggg gatccgcgtt ccctcgtaga tcctcggacg ctcgggggcg acggccactt
     5281 attcctctcc cgagaaacgg accggctcgc cgccgatcgg gtagtccttg cgcagcgggt
     5341 ggccttccca gtcgtcgtcc atgaggatgc ggcgctggtt ggggtggccg tcgaagcgaa
     5401 tgcccatgag gtcgaaggcc tcacgctcgt gccagtccgc agtcggccag acgcccacga
     5461 ggctcggaac gtgctcgccg tcttcgaccc acgcctgcag tcgcacccgt ggcgcgccgg
     5521 ctcgcatccc gagcaggtgg tagctgacgg agaagcggtg cggcttctgt cgcggcaggc
     5581 gctggtgccc ctgcgtcgca ggctcgttca ggtcgcgacc gcgaggcgtg ccgatgtagc
     5641 cggacacgcc ttttgcgccc caaccgaggt agtcggtagc ggcgacgtcc gagagaaagt
     5701 tgaagccccg ctcgtcacgc aggtgcaggg ccgcgtcctc gagacgcggc gtgtcgacga
     5761 cgatcgtcgt ctcgccgtgc gcttcgtgaa cctccacgag cccggggacg cggttgtagt
     5821 tcaggatgcg accccgcgga tctcgtaagc cggcgggatc ccggccatca ctttctcgtg
     5881 gagccgcacg atgccctcca tcagcgcctc cggccggggt gggcatcccg gcacgtgcac
     5941 atcgaccgcc acgatcttgt ccacgccctg gagcgtcgtg tagttgttga acatgccgcc
     6001 ggagctggcg cacgcgccca tggcgatgac ccacttcggc tcgagcatct ggtcatagac
     6061 ctgccggagc ggcgccgcca tcttgtgcac gacgcggccg gacacgatca ggaggtcggc
     6121 ctggcgcggc gacgaacgga aggcctccat cccgaaccgc gcgatgtcat agcgcgacga
     6181 gacgatcgac atcatctcga tcgcgcagca ggcgagcccg aacgtgtcgg gccacatcga
     6241 cttcgactgc gcccaggcga ccgccttctc gagcgtcgtg aggccgacgg cctgctccat
     6301 cgcagcgacc tcttgctcga acacgcccgg ccccttcgtc ggccagagca agcgttcgga
     6361 ctggagcccg tggtcgcgga gcttcttccc gtcctctatt gccaatcgag tgcgcccttt
     6421 cgccagacgt agacgtacgc gaccacgagg atcgcgatga agaagaggaa ctcgaggaag
     6481 ccgaaccacg caagctggtg gaggatgacc gcgagcggat acaggaagac gatctcgatg
     6541 tcgaagacga tgaagagcat cgccgtgaga tagaagctca ccgtgaagcg ctgcactctc
     6601 ggagcgccgg tggagacgcc cgactcgaac ggaagctcgc gccagcgcgt gtgcttgtgc
     6661 ggtctcgtcg cgaagacgaa ggagaaccac atcatcgatg cgggaatgat cagcgcgaaa
     6721 atcgcgtaga tcaggaacgg cagccagttg tcgagcacgc gtccctcccc ttgggcgacg
     6781 atgaacctaa caggttcccc tccaacgcca cgaagcatgg cgaaaaccgc tcaaccgtgc
     6841 ctgagacgtg tgttacaaac tgccccttcc ggagtaccct gggaaccgtc cacgttgcta
     6901 cgatcagatc acgatggagc tccaggaaag agtcgtgccc ctgatgaccg tcaccgagtc
     6961 cgcggctgtc aagatcaagc agctgatcgc cgaggaggcg gacgtcagcg tcctccgcgt
     7021 cgccattcag ggcggcggct gctcgggctt ccagtacggg ctcggcttcg accgcggcgc
     7081 cgccgacggc gacctcgagt tcgagatgca cggcgtgcag gtcgtcgtcg atccgttcag
     7141 cgcgccgtac ctcaagggcg ccgagatcga ctacgtcgac tccatccagg cggccgggtt
     7201 cgcgatcaac aacccgaacg ccagctcctc ctgcggctgc ggccactcct tccaggtcga
     7261 agaaggcgcc gaggccgaag atgccgtcgg cggctgcggt tccggctgta gcgcgtagcg
     7321 aaagctgcgc tttcgctccg atttgtaggt acgccgcttc gcgggaaccg tgcgcgggcg
     7381 aagcccccgc acccctcaaa gaccgggctg gccgaccggt gtagtcccaa ggtggcgcga
     7441 ttacgatcgg caccgtgagc gtaaaccccc ttcggatcgc tgtggtcggt tccggtccgg
     7501 ccggcttcta cgcggccggc gcgctgctcg acgccgaagt atcggtcgac gtggacatga
     7561 tcgagcggct gccgacaccg tggggcctcg tgcggctgcg
//
LOCUS       DASVEU010000034         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1191231,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000034 DASVEU010000000
VERSION     DASVEU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191231"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..403
                     /locus_tag="VF225_01285"
     CDS             125..403
                     /locus_tag="VF225_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143916.1"
                     /translation="MTETRTFASDTSAAPVAQPAPSQPARADKGKGKTKRHDKAEQTS
                     KPAEPVQAAAPVATTTTTPAETSPQPECDKEHGNDKEKGKDKDKGHDG"
     gene            complement(613..>2443)
                     /locus_tag="VF225_01290"
     CDS             complement(613..>2443)
                     /locus_tag="VF225_01290"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7143917.1"
                     /translation="EAVDPVVEGRARAEQTDRSTRYGLHDPNVALPVLIHGDASFPGQ
                     GGVAETLNLSALEGYSTGGTLHLIANNQVGFTTDPSEGRSTRYSSDLAKGFDVPIIHV
                     NADDPEAAMSVVSLALAFRRRFESDVVIDLVGYRRHGHNEQDEAAYTQPLMTAQIAEH
                     RTVRDQFAKQLVAEGVVTEDEGDDLFAQVTSELKQAHEALKATFGAPLPAEGKIPFSS
                     GDGVVTAVSADRLRELNDELLRVPEHFTVHPKLLGQLQRRLKTIAEGGIDWGQAEALA
                     FGSLLVEGIPIRLTGQDTQRGTFSHRHAVLHDVHTGETFTPLQNLGEASASFEIYNSP
                     LSEYACLGFEYGYSIAAPEALVLWEAQFGDFVNGAQIVIDQFIVAGLAKWRETTRLTL
                     LLPHGYEGNGPEHSSARLERFLQLAAQENIRVANPSTSGQHFHLLRRQALDAAGRPLV
                     VMTPKGLLRLKQASSTLADLAEGAFQQVIDDPSATHAEVTRLVFCSGKVYYDIAGHEA
                     RAQATQVAVARLEQLYPFPVDAYAALIARYPNLREVVWTQEEPQNMGAWRTIRHRLEA
                     PLKEAGVPLSYAGRPWRASPSEGYPTAHLREQDRIVRAALGTG"
BASE COUNT          406 a          811 c          837 g          389 t
ORIGIN      
        1 cgccgtcccc gcggcggggc ctgctcggtc tcccggtcgg agttccagtc ctgcgcgcga
       61 gccgaggccc gccccgaggc ccgctccgag gcccgctccg cagccgacgc caccggcggc
      121 gccggtgacc gagacgcgca cgttcgcgag cgacacctca gcggctcccg tcgcgcagcc
      181 ggccccgagt caaccggccc gtgccgacaa gggcaagggg aagaccaaga ggcacgacaa
      241 ggccgagcaa acgagtaagc ccgcggagcc cgtgcaggca gccgcgcccg tggcgaccac
      301 gacgacgacg cctgccgaaa cgagcccgca gcccgagtgc gacaaagaac acggcaacga
      361 caaggagaaa gggaaagaca aggacaaagg gcacgacggc tagtgcctgt gcgaggatag
      421 ttccgtgcgg ccacgcctca ccagacgcct cgggcccgtc ggtcttgcgc tgaccgcttg
      481 ggacatctgg cggcgactcc cgccgaaaca acgcaagcag gtcttgaacg tggctcgcaa
      541 gcatgggccg aaggtcgcgt cgcgtgtttt gaaggcgagc gcgcgggcgc gttcgcgccg
      601 cgcacgtaag cgctagcccg ttccgagggc agctcggacg atgcggtctt gctcgcgcag
      661 gtgggcggtc ggatagccct cgctcgggct tgcccgccag ggtctgccgg catagctgag
      721 cgggactccc gcttccttca gcggcgcctc cagccgatgc cggatcgtcc gccaggcgcc
      781 catgttctgc ggctcctcct gcgtccagac gacctcgcgc aggttcgggt aacgggcgat
      841 gagcgctgca tacgcgtcga cggggaatgg atagagctgc tcgagcctcg cgaccgcaac
      901 ctgcgtggcc tgcgcgcgtg cttcgtgccc ggcgatgtcg tagtacacct tccccgagca
      961 gaagacgagc cgagtcactt cggcgtgcgt tgcgctcgga tcgtcgatca cctgttggaa
     1021 cgcgccttcc gcaaggtcgg cgagcgtcga cgaggcctgc ttcagacgca agagcccttt
     1081 cggcgtcatc accaccagcg ggcggcccgc tgcgtcgagg gcctgacgac ggagcaggtg
     1141 gaagtgctgg ccggacgtcg acggattggc gacgcggatg ttctcctgcg ctgcgagctg
     1201 gaggaaccgc tccagccgtg cgctcgaatg ctcgggaccg ttcccttcat agccgtgggg
     1261 tagcaggagc gtcagccgcg tcgtctcacg ccatttcgcc agtcccgcga cgatgaactg
     1321 gtcgatcacg atctgtgcgc cgttgacgaa gtcgccgaac tgggcctccc agagcacgag
     1381 cgcttccggc gcggcgatgg agtacccgta ctcgaagccg agacacgcgt attccgagag
     1441 cggcgagttg tagatctcga aggacgcgct tgcctcgccg aggttctgga gcggcgtgaa
     1501 cgtctcgccg gtgtggacgt catgcagcac ggcgtggcga tgagagaacg tgccgcgttg
     1561 agtgtcctgg cccgtgagcc tgatcgggat tccctcgacg agcagggacc cgaacgcgag
     1621 agcttcggcc tggccccagt cgattccgcc ttcagcgatc gtcttgaggc gccgctggag
     1681 ctgacccagc agcttcgggt ggacggtgaa gtgctcgggg acgcgcagca actcgtcgtt
     1741 caactcgcgc aaccgatcgg ccgagacggc ggtgactacg ccgtcgccgc tggagaacgg
     1801 aatcttccct tctgcgggaa gcggcgcgcc gaaggtcgcc ttcaatgctt cgtgagcctg
     1861 cttcaactca gaagtgacct gcgcaaacag gtcgtcgcct tcgtcctccg tgacgactcc
     1921 ctccgcgacg agctgttttg cgaactggtc gcgcacggtc cggtgctccg cgatctgcgc
     1981 ggtcatcaac ggctgcgtgt aggcggcttc gtcctgctcg ttgtggccat gccggcggta
     2041 gcccacgaga tcgatgacga cgtcgctctc gaaccggcgg cggaaggcca gcgcgagaga
     2101 gaccacggac atcgcggcct ccgggtcgtc ggcgttgaca tggatgatcg ggacatcgaa
     2161 gcctttggcg aggtcgctcg aatagcgcgt cgagcgccct tcgctgggat cggtcgtgaa
     2221 accgacctgg ttgttggcga tcagatggag cgtcccgccg gtcgagtagc cctcgagcgc
     2281 ggacaggttc aacgtctccg cgacgccccc ctggccgggg aacgaggcgt ccccgtggat
     2341 cagcaccggc agggcaacgt tgggatcgtg caagccgtag cgtgtcgagc gatcggtctg
     2401 ctctgcacga gctcgtccct cgaccacggg atccactgct tcc
//
LOCUS       DASVEU010000035         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_26028,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000035 DASVEU010000000
VERSION     DASVEU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26028"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..732)
                     /locus_tag="VF225_01295"
     CDS             complement(103..732)
                     /locus_tag="VF225_01295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7143918.1"
                     /translation="MLGARPFALAEDPLLRSTTDRIAAQLAILPPKLQLLDDGFPRAF
                     VVGRGPRSSTLVVSSGLLTALTPNELGAVLAHELSHVRSRDVLTQTYAVLLSTTLLEL
                     TRLGGFLSRFLMAVLAPVASAFTHLMLSSKREFAADRTGAALGDPNDLADALIRLDRA
                     AELVEFTASPATEPLYTVSPFDHEERVARMFVTHPPLDDRVARLRPIPE"
     gene            complement(927..1193)
                     /locus_tag="VF225_01300"
     CDS             complement(927..1193)
                     /locus_tag="VF225_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143919.1"
                     /translation="MEDYTNKYKGEQIELALFGGEYQEGVLTAYCSIEDVPHVELNGH
                     ILVPLQNVASLHCSSRCDAPEPDWPPRGLEELDRDDRPDEEEEG"
     gene            complement(1631..2140)
                     /locus_tag="VF225_01305"
     CDS             complement(1631..2140)
                     /locus_tag="VF225_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7143920.1"
                     /translation="MEQVGRSDAWTTSLAADRVGFVIEELAARIGMRLTRPSPNTFVM
                     NHGSNPRARAFGVWFEPSSLPSKAHIEISAAPGGTRLTVRIEETWPNYLEEKSRTEYA
                     ALFDQWLQALREAIPALPDEPGIQGHDIAGELARLAEVHAQGVITDDEFSAAKAAVLA
                     RPDTAGSGP"
     gene            complement(2326..2595)
                     /locus_tag="VF225_01310"
     CDS             complement(2326..2595)
                     /locus_tag="VF225_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143921.1"
                     /translation="MYAVVGRSTIEDFDQARKFLREEGIPRLSQMPGFVSGHWVRLGE
                     NTGASMIIFETEEAARTAKERFEANPPPTVSPMSLEIAEVLEHAP"
BASE COUNT          449 a          890 c          912 g          425 t
ORIGIN      
        1 gagcttgtcc gagaagaccc atgagacgac gccgtacccg gcgagcgcga agaggatgac
       61 cacgatcggg atcacgaccc agcgccggcg catcgtcgga ggctactcgg ggatcggtcg
      121 cagccgcgcg acccggtcgt cgagcggcgg gtgcgtcacg aacatgcgcg cgacgcgctc
      181 ctcgtggtcg aacgggctca cggtgtagag cggctcggtc gcgggcgacg cggtgaactc
      241 cacgagctcc gcggcgcgat cgaggcggat gagcgcatcc gcgaggtcgt tcgggtcgcc
      301 gagcgccgct cccgtcctgt ctgccgcgaa ctcgcgcttc gacgaaagca tgaggtgggt
      361 aaaggccgac gcgacgggtg cgagcacggc catgaggaac cgcgagagga agccgcccag
      421 ccgcgtcagc tcgagcagcg tggtcgagag gaggacggcg taggtctggg tgaggacgtc
      481 gcgactgcgg acgtgcgaga gctcgtgcgc aagcacggca cccagctcgt tcggcgtgag
      541 tgccgtcagg aggccggagc tgaccacgag cgtcgagctc cgtggcccgc ggccgacgac
      601 gaacgctcgc gggaagccgt cgtcgagcag ctgcagcttc ggaggcagga tcgcgagctg
      661 ggccgcgatc cgatccgtcg tcgagcgcag cagcggatcc tcggcgagcg cgaacggccg
      721 cgcaccgagc atcccgagca acgcgcgatc gccgaccgca tacgcggcgc tcgccgcgag
      781 gagcgagcag atgacgaaga gcgtggcgcc gcggcggtcc gcgagcagcc agccgatccc
      841 gccgaagacc gcggcgagca gcgtaacgag gagccaggcc ttgagaaggt tgcgcgccgc
      901 ggcaccgcgc gcgccgaccg gctcggtcag ccttcctctt cctcgtccgg acggtcgtcg
      961 cggtcgagct cctcgagacc gcgcgggggc cagtcgggct cgggggcgtc acagcggctc
     1021 gagcagtgga ggctcgcgac gttttgcagc gggacgagga tgtggccgtt gagctccacg
     1081 tgcggcacgt cctcgatcga gcagtacgcc gtcaggacac cttcctggta ctcgccgccg
     1141 aagagcgcga gctcgatctg ctcgcccttg tacttgttcg tgtagtcctc cacggcccgc
     1201 aggctagccg aacccgcata aaacggtggc cgtcccggcc gtaaaggtcg ctagatgcgc
     1261 tgctggtggt gcaccggtgg tgcattggga gtgtctgcag cgcagacaac gctaccggcg
     1321 acgattgcgc tccttggcca gcagcgcgag tgagcaggtt ggtgccggcg ttcaggcagc
     1381 ccgcccggcc cgcgctaccc ctggatgacc caccaggcga gcccgctcac gaccatgccg
     1441 taggtaacga acctcgcgta ggagattgtt ttgacagcct cggctcccgc gacgacgagg
     1501 aagatcgcac cgtactgggc agcacggcac gggctcctcg agtcccggcg gcgggtcgtc
     1561 gcgccagaag gatcgtctcg cgcttccgtt tttcccacgt ggatgaggcg ctaggcgggc
     1621 ggcgggggcg ctacgggcct gaaccagcgg tgtcgggtcg cgctagcacc gcggccttcg
     1681 cggcggagaa ctcgtcatcc gtgattacgc cttgcgcgtg aacctcggcg agccgagcga
     1741 gttcgcccgc gatgtcgtgt ccttgaatcc caggctcgtc aggcagagcc ggtatcgcct
     1801 cgcgaagagc ctgcagccat tggtcgaaca aggcggcgta ctccgtcctt gacttttcct
     1861 cgagatagtt gggccaggtt tcctcaatcc gaacggttag ccgggtgccg cccggagccg
     1921 cgctgatttc gatgtgcgcc ttagagggca acgagctcgg ttcaaaccag acgccgaagg
     1981 cgcgagccct tgggttggag ccatggttca tcacaaatgt gttcggactc gggcgcgtca
     2041 gcctcatccc tatcctggcc gcgagctcct cgatcacaaa gccgaccctg tcggctgcca
     2101 gcgaggttgt ccatgcgtcg ctccgcccga cctgttccat gtgcggagcg tatgaaatcg
     2161 gacgcgctcc gacaacgagc acagcgaacg tcaactgcgt cgtgtgcgcc gacctgcgcg
     2221 tagctaggcg tcgtgacggc tactaacggc cgcagaccac caccgcgctg ccccgtagca
     2281 gttccgctcc gaaatggagc cgtccgaatc agacccaacg tgctgtcagg gcgcgtgctc
     2341 cagaacctcg gcaatctcca ggctcatcgg actgacagtg ggcggcgggt tcgcctcgaa
     2401 ccgctccttc gccgttcgcg ccgcctcctc cgtctcgaag ataatcatcg acgcaccggt
     2461 gttctcgcca agccgcaccc aatgcccgct gacgaaaccg ggcatctgcg agagccgcgg
     2521 aatgccctcc tccctcaaga acttccgtgc ctggtcgaag tcttcaatcg tgcttctgcc
     2581 taccaccgcg tacatgcgtg cctcccttgt cgtgggcggt gtggcctcaa gtccgatcta
     2641 gacgatgcca cggacagggc ggggttgcta gtagcc
//
LOCUS       DASVEU010000036         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_415698,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000036 DASVEU010000000
VERSION     DASVEU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415698"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VF225_01315"
     CDS             <1..1512
                     /locus_tag="VF225_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7143922.1"
                     /translation="QDADHVLVRTAHCIDGTAEIDLVCEPAFDYGRVPAEWTLTADRH
                     RAEATGAGQAIQLQTDMLVGIEAGRARARHVLRAGETVYCALAWSDEALVPTTADEAA
                     DQLAATTRFWRGWLARAAIPDHEFGPMIQRSALTIKGLTYMPTGAAVAALTTSLPETP
                     GGERNWDYRYSWLRDSTFLLRALHYLLLDWEADEFMQYMADLERNDDGGLQIMYGIDG
                     RRDLTESVREDLSGYDGAYPVRIGNGAFDQRQNDVFGAALDAVLLHSRRSQRIPRRLW
                     PLVQAQAECATAVWRQPDQGIWEARGEPQQYVSSKLMCWVALDRAARLANIRGEAALS
                     AEWAHTAAEIKSDIVEHGVDGRGVLRQHYETDALDAATLLAAIFGFLPGDDTRLRATV
                     LAVEDELTEGGYVLRYRTGETDDGLSGKEGSFLICSFWLVSALAIIGETQRARHLMER
                     LLKVASPLGLYAEEFDADTGRHLGNFPQAFSHLALVEAAARLILAEGLAEVAG"
BASE COUNT          327 a          698 c          685 g          307 t
ORIGIN      
        1 caggacgccg accacgtgct cgtgcgcacc gcgcactgca tcgacggcac ggccgagatc
       61 gacctcgtgt gcgagccggc gttcgactac ggtcgcgtgc ccgcagagtg gacgctgacc
      121 gcagatcggc atcgcgccga ggcgaccgga gccggccagg cgatccagtt gcagaccgac
      181 atgctcgtcg gcatcgaggc cggccgcgct cgagcccgcc acgtcctgcg cgccggcgag
      241 acggtctatt gcgcactggc gtggagcgac gaggcgctcg tcccgaccac cgccgacgag
      301 gccgccgacc agctggcggc gacgacgcgc ttctggcgcg gctggctcgc gcgggccgcg
      361 attcccgacc acgagttcgg gccgatgatc cagcggtcgg cgctcacgat caaggggctc
      421 acgtacatgc cgacgggggc ggcggtagcg gcgctcacga cctcgctgcc ggagacgccc
      481 gggggcgagc ggaactggga ctaccgctac agctggctcc gcgactcgac cttcctcttg
      541 cgcgcgctgc actacctgct cctcgattgg gaggccgacg agttcatgca gtacatggcc
      601 gacctcgagc gcaacgacga cggcggcctc cagatcatgt acgggatcga cggtcgccgc
      661 gatctgaccg agtccgtgcg agaggacctc tccggctacg acggggcata ccccgtccgg
      721 atcggcaacg gcgcgttcga ccagcggcag aacgacgtct tcggtgcggc gctcgacgcc
      781 gtcctgctcc acagccgccg cagccagcgg attccgcggc gcctgtggcc gctcgtccag
      841 gcgcaagccg agtgcgcgac agcggtgtgg cgtcaaccgg accagggcat ctgggaggcg
      901 cgcggcgagc cgcagcagta cgtctcgtcg aagctgatgt gctgggtcgc gctcgaccgc
      961 gcggcgcgtc tcgcgaacat ccgcggagaa gctgcgctct cggccgagtg ggcgcacact
     1021 gccgccgaga tcaagtcgga catcgtcgag cacggggtcg acgggcgcgg ggtgctgcgc
     1081 cagcactacg agaccgacgc gctcgacgcg gccacgctgc ttgcggcgat cttcggcttc
     1141 ctgccgggcg acgacaccag actgcgcgcc acggtgctcg ctgtcgaaga cgagctgacc
     1201 gagggaggct acgtgctgcg gtaccggacc ggagagaccg acgacgggct gtccggcaag
     1261 gagggcagct ttctcatctg ctctttctgg ctggtatcgg cgctggcgat catcggcgag
     1321 acgcagcggg cgagacacct gatggagcgc ctcctgaagg tcgcatcccc gctcgggctt
     1381 tatgcggagg agttcgacgc cgacaccggc agacacctcg gcaacttccc gcaggccttt
     1441 tcgcacctcg cactggtcga ggctgccgca cggctcatcc tcgcagaggg cctcgcggaa
     1501 gtggccgggt gactgcctga ggactgccgc caactacgca agcttgcgcc gttcgcgtcc
     1561 accggcgcgt ctacgccggg gcacatggcc cgccgcgcga cccaccgatc tccgagcacc
     1621 gacaacgcga gtcgggtgcc cattcggtgc ccaacctccc gtggtcccgg tgaatgccgg
     1681 gttgccttgg gacccctagg gacgagaaac ctcccctttg caggcatatc cgaaggaatc
     1741 ggccctgcat gttctgcttg ccatgcagaa ggtcgagggt tcgaatccct tcagccgctt
     1801 ccctggatca ggctctacag agcctcaaac cgtgcctcgg cggtcctgcg acgtgatacc
     1861 cggtccgagc ctcgagatcc tcggggagga caagattggt cgtctgtgtt ggcatcgaac
     1921 ctcctcctga agaagaggtt cgatgccgaa cacaatggcg tcactcgcgc agtgagctga
     1981 atgcggcccg cacctcggtc gagaagagct ccggctc
//
LOCUS       DASVEU010000037         8447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_998090,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000037 DASVEU010000000
VERSION     DASVEU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998090"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..919)
                     /locus_tag="VF225_01320"
     CDS             complement(182..919)
                     /locus_tag="VF225_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7143923.1"
                     /translation="MAAAIEVAGISKSFRIPHEQRTYFKEYFLHPFRRTTYERNDALQ
                     SVTFSVDAGEFFGIFGPNGSGKSTLLRILAGIYVPDAGRVRVNGLLSPFIELGIGFNL
                     ELNARDNIRINSTLAGLTKRQLDEKFDDILEFSELERFVDQKLKNYSSGMLMRLAYSI
                     AIQIPFDVLLLDEVLAVGDASFQEKCFTTFERFKNEGKTVLLVTHAVELLSRFCDRVL
                     LLESGVVRTIGRAEDVVESYREGALVG"
     gene            complement(920..1762)
                     /locus_tag="VF225_01325"
     CDS             complement(920..1762)
                     /locus_tag="VF225_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143924.1"
                     /translation="MSGISYSSRRVRATSGLPYYLRVLRVVGAIEFKAKYAGAALGYV
                     WSVAKPLAYFGVLWLVFAHLLRTANQTHDFTIFLLVGILLYTFFVDTVGAMLPSIVTG
                     GSILRRLAFPPILIPLSASVGIAITFCANMTALAVFIAIQKVTPRLEWVLVPALLAEL
                     CIFCVGIGLLVAALYVRFRDVVQIWELTAQLLFFASAIFYPIGILPAWAQKVAFLNPF
                     VQVMQDVRHAVLGGPSGPNDVTAATVYGGAAGRLIPILIAVFIFLGALAFFRREGRYF
                     AERV"
     gene            1849..5217
                     /locus_tag="VF225_01330"
     CDS             1849..5217
                     /locus_tag="VF225_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7143925.1"
                     /translation="MRLTTPRSDLPAEPRVAGTASGARSGALLAVASGAGIVAAYVFL
                     LAAGRILGSEDYGSLAALLGLLAIVFIPAGALQMAVSREVSRSVATGDPAGAARLARG
                     TLRAALIATAPLLAVALALAKPLAHVLHIHSVAMVVLALLSLSTALVFPLAMGVLQGQ
                     QRFSDLAILYVLPWLVRLVVLAIAAAAGYRLGGAIFATLAGALAGTALALMLIREPLR
                     GAGALPRPELVTFLRYLWPVAVGLVGIALLTNVDILIVKARFSAHDAGAYAAASAFAR
                     VGFFLPATILTVLFPRTAARQARGEETEDILGRSLLATTAFCGLLVLVYAAAGVGLVS
                     MTFGTDFSKGGHLLAPFALAIGFYSLANVLVGYHLSRGETRYAWIVAGAVVVQVVALV
                     LVPSSLRGVVWTNVVVGALLLAAHELFVGSSVPALRAALGRVSAATRVRARRVAGETA
                     LVLFGSTLFICALMWPIVRHLSSTIVGSPGSDSTGSVAFLWTLQHETGYHLLGTTHHT
                     FSGAPFGWDEGNGLNIQWSLPYYPAYLATKLFGAVAAYNLITLAGYALSGASMYLLVR
                     YLRCSRLVAAWAGMVFIIFPWHFARTEHASLTHLEVLALLVLALVAAARNPTWIRFTF
                     VGVATLACWLTSGYFGGMAVITVIAFSFGAALTAPRHRALLLAGGAIGAAFLASGLVA
                     IGSYASGVNAGAGIHRDASALMAYGLRPIELVVPTANHLVFNLDSFWLRHEHGSNPTE
                     ISNYLGLLTIALAVGWVVVVLRRRNGMATVTAGLIAAFVVGFLFALPSPVAGISMPSK
                     LLWNVLPAFRVPSRWDPLLMTALLPLAALGLQTLTGTIERRRRFASVAIVGAALLLSF
                     VELSTNRPYHFRTVPVPPEYAAIETKTPNGILAEYPLGYSDIYRLWQRVHERPLVNGA
                     PEGSVADQVRLMILDPAQAGTAQTLSLLGVTAVAIHPGGPADTPLQPREPRPADGYRL
                     VGRFPDRSSVWAVTAPPATALVTLPGGFATPRLVAGKVGYPLVAPSGVALLELRAKEH
                     GVIRLVFDATAPSGSRNFRIQDSVGEHPFPLSGSMHFDLNVEVPRGVSQLLLKIDPAP
                     ASEADAIVLSQPRAEPASGAAALHASPSSADPGF"
     gene            complement(5197..6282)
                     /locus_tag="VF225_01335"
     CDS             complement(5197..6282)
                     /locus_tag="VF225_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX7143926.1"
                     /translation="MILVVFGTTGELIKLAPVLLRLDDRSHPYLLATTAQQVQQIPSF
                     LEQFGLRQPDMWLARGAEGRDLRVNSDIPGWVATVARSWVPARRRLRHVPLVLVHGDT
                     MTTVLGSLMGRTLRATVAHIEGGLRSYDWRHPFPEELNRKLATALSRIHYAPGAWAAG
                     NLRRGDIVDTGSNTIRDSLELVTDAEPALELPAAFGVVSLHRFELLNNRRLLTETVEV
                     LADAARTTPLLFIDHPVTSAALERFGLTRFFDASTFMRIPRLRFFDFVRVERRSSFVI
                     TDSGGSQEECYYLDLPCLVHRVKTERREGLGENAVLSQMSADVLRDFLDDPGRYRRTT
                     TLAVASPSDVIVGDLERRGFVSKAGVG"
     gene            complement(6279..7247)
                     /locus_tag="VF225_01340"
     CDS             complement(6279..7247)
                     /locus_tag="VF225_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7143927.1"
                     /translation="MERASLSVVIPVYNEAVHLSATMRALVAALDESDFDAEVVVVDD
                     GSTDGTADAARASADGGIAVRIVAQPNRGRFEARRAGLAHARGEYVLLLDSRVRLAPG
                     GLRFVHERLQSAPVWNGHVHVDADNAWGEFWRLLAELAWRDYFDDPRTTSFGIAEFDR
                     YPKGTGCFLAPRALLESALAGFRSHYGDVRLANDDAPVLRAIAAAERIHISPEFACTY
                     SPRTTFDAFLRHAIHRGTVFLDGHGTPESRFFPAVVAFFPLSVALLRRPRLIPAALGA
                     CGAAAAAYGAWAGRSKGEVRTLAAVTPLYALGHGLGMWRGVLELLR"
     gene            7281..8132
                     /locus_tag="VF225_01345"
     CDS             7281..8132
                     /locus_tag="VF225_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7143928.1"
                     /translation="MLGRYWWEVVRRLDRPGWRHLLVVPGSVWVSFMYRAPCLVYWRN
                     GAWIHRYRGAKIPHAGLGRAAPPAVFTRDARDTALYVYTPDQGDVVFDVGAGIGGETL
                     LFSRLVGASGRVVSIEAHPSTYGRVVELCNANGLANVTPLQVAAAESDGTATITDLAN
                     HVRNTTLAAARDGIEVPARRLETIAKSLDITYIDLLKVNIEGAEVSALRGLGPLLAKT
                     RYVCIACHDFLADEGGAETLRTKAAVRELLLEHGFELTSRDEAAAPWTRDYVYGVNTR
                     LEPHADD"
     gene            complement(8233..>8447)
                     /locus_tag="VF225_01350"
     CDS             complement(8233..>8447)
                     /locus_tag="VF225_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143929.1"
                     /translation="GIALVLAYVAALVLSGLHAAVRFRSLLVGAAEPFAVVATQAVYL
                     VGFVRGLVSSSASASPNRTFQSKRRA"
BASE COUNT         1417 a         2869 c         2754 g         1407 t
ORIGIN      
        1 ttgtacgagg cgcgcaatgc gcttacgcct cgagacgaag gaacgaatcg gagtcatcag
       61 tggccataac gcgcggctag ccgcgcgcag gatcaaaatc ggcagattgc tcatcgacgc
      121 gtgtcgcgtt cggcttcgca cgactagagg ccgcctcgaa ggtgccccaa cccgcgtttc
      181 gtcatccgac gagggcgccc tctcgatagc tctcgactac atcctctgcg cgtccgattg
      241 tgcgaacgac gcccgactcg agcagaagga cgcgatcgca gaaacggctc aacaactcaa
      301 ccgcatgcgt cacaagcagg accgtcttgc cctcattctt aaacctctca aacgtcgtga
      361 agcacttctc ttggaagctc gcgtctccca ccgccagaac ctcgtcgaga aggagaacgt
      421 cgaacgggat ctggatggcg atcgagtacg cgagccgcat gagcattccc gacgagtagt
      481 tcttcagctt ctggtcgacg aaccgctcga gttcggagaa ctcaaggatg tcatcgaatt
      541 tctcgtcgag ttgccgcttg gtcaaccctg ccaatgtcga attgattcgg atgttgtcgc
      601 gggcgttgag ttcgaggttg aagccgattc cgagctcaat gaagggagag agaagcccgt
      661 ttactcggac gcgtccagcg tcgggaacgt agatgccggc gaggatcctg aggagcgtgc
      721 tcttcccgct tccattggga ccgaagatgc cgaagaactc gccagcgtcg acggagaagg
      781 tgacgctctg caaagcgtcg ttgcgctcgt acgtcgtgcg acgaaacgga tgcaggaagt
      841 attccttgaa gtacgttcgc tgctcgtgag gaatccggaa cgacttcgag atccccgcga
      901 cttcgattgc cgctgccact cagaccctct cagcgaagta tctaccctcg cggcgaaaga
      961 acgcgagagc gccgagaaag atgaacacgg cgatcagaat tggaatgagc ctgccggctg
     1021 ctcccccgta cacggtggcg gcggtcacgt cgtttggacc gctcgggcct cccagtaccg
     1081 cgtgccggac gtcctgcatg acctggacga acgggttcag gaatgcaact ttctgagccc
     1141 aggccgggag gatgccgatc ggatagaaga tcgcggaggc gaagaacaac agctgcgcgg
     1201 tgagctccca gatctgcacg acgtcgcgaa accgtacgta tagcgcggcc acgagcagcc
     1261 cgatgccgac gcagaagatg cacagttcgg cgagtagcgc cggaaccaag acccactcga
     1321 gtcgaggcgt aaccttctgg atcgcgatga agacggctag cgcagtcatg ttcgcgcaga
     1381 atgtgatggc gatgccgacg gaggccgaga gcgggatcag gatcggtgga aaagcgaggc
     1441 gtcgcaggat cgaacctccc gtgacgatcg acggcagcat cgccccgacc gtgtccacga
     1501 agaacgtgta gagcaggatt ccgaccagga ggaagatcgt gaagtcgtgc gtctggttgg
     1561 ccgtcctcaa gaggtgtgcg aaaacgagcc acaacactcc gaagtacgcg agcggcttgg
     1621 cgacggacca gacgtagccg agagcggcac cggcgtactt ggccttgaac tcgatggctc
     1681 caacgacacg gagcacccgt aggtagtacg gcaacccact cgtggctcga acgcgtcgcg
     1741 atgaatagga gatcccgctc acctgtgcaa aggctagcgg cggtggcagg cgtctttgcc
     1801 tgacgccctc ctgcgcccct cgaccggagg tttgcccaga atgggccgat gcgcctgacc
     1861 accccgcgat ccgatctccc agcggaaccg cgcgtcgccg gcaccgccag cggcgcgcgc
     1921 agcggcgccc tgctcgccgt cgcgagcggc gcgggcatcg tcgcggccta cgtcttcctg
     1981 ctcgctgccg gccgcatcct cggcagcgag gactacggct cgctcgccgc gctgctcggc
     2041 ctgctggcga tcgtgttcat tccagccggg gcgctccaga tggcggtgtc gcgagaagtt
     2101 tcgcggagcg tcgcaaccgg cgatccggca ggggctgcac gcctggcccg agggacgctg
     2161 cgggctgctc tgatcgcaac cgcgccattg ctcgccgtgg cactcgcact cgcaaagccg
     2221 ctcgctcacg tcctccacat ccactcggtg gcgatggtcg tcctagcgct gctgtcgctg
     2281 tcgaccgcgc tggtgttccc gctcgcgatg ggcgtcttgc aggggcagca acgtttctcc
     2341 gacctggcga tcctgtacgt gctcccgtgg ctggtccgcc tcgtggtcct cgcgatcgcc
     2401 gccgccgccg gctatcgcct ggggggcgcg atcttcgcga cgctggccgg agcgctagca
     2461 ggcaccgcgc tggcgctgat gctcatccgc gagccactac gcggcgccgg ggcactcccg
     2521 cgacccgaac tcgtgacgtt cctccgttat ctctggcccg tcgccgtcgg cctcgtcggc
     2581 atcgccctgc tcacgaacgt cgacatcctc atcgtcaagg ctcgcttctc ggcccacgac
     2641 gcgggcgcgt acgcggctgc ctcggcgttc gcacgcgtcg ggttcttcct gcccgcgacg
     2701 atcctgaccg ttcttttccc acgcacggcc gcgcgccagg cgcgaggcga ggagacggag
     2761 gacatcctcg ggcgctcgct cctggcgaca acagcattct gcggactgct cgtgctcgtg
     2821 tacgccgccg cgggcgtcgg gctcgtctcc atgacgttcg gcaccgactt ctcgaagggc
     2881 ggccatctgc ttgctccctt cgcgctggcc atcggtttct actccctcgc caacgtgctc
     2941 gtcgggtacc acctgtcgcg cggggagacg cgctacgcct ggatcgtcgc cggggcggtc
     3001 gtcgtccaag tcgtcgcgct cgtactcgtg ccgtcaagcc tgcgtggcgt cgtgtggacg
     3061 aacgtcgtcg tcggggcgct gttactcgca gcgcacgagc tgttcgtcgg ctccagcgtg
     3121 ccggcgctgc gcgcggcgct tgggcgtgtc tccgcggcga cacgagtccg agcgcgccgc
     3181 gtggcgggcg agacggcgct cgtcctcttc ggctcgaccc tcttcatctg cgcgctcatg
     3241 tggccaatcg tcaggcacct cagctcgacg atcgtcggct cgcccgggtc ggactcgacc
     3301 ggatccgttg cgttcctctg gacgctgcag cacgagacgg gctaccacct gctcggcacg
     3361 acgcatcaca cgttcagcgg cgcccctttc ggctgggacg aaggaaacgg tctcaacatc
     3421 cagtggtcgc ttccgtacta cccggcctac ctcgcgacga agctgttcgg cgcggttgcc
     3481 gcgtacaacc tgatcacgct cgcgggttac gccctctcgg gcgcatcgat gtacctcctg
     3541 gtgcgctacc tccgctgcag tcgcctcgtc gccgcgtggg cgggcatggt cttcatcatc
     3601 ttcccctggc acttcgcacg caccgaacac gcctcgctga cgcatctcga agtgctcgcc
     3661 ctgctcgtgc tcgccctggt tgccgctgcg cgaaacccga cctggatccg gttcaccttc
     3721 gtcggcgtcg caacgctcgc atgctggctg acgtccggct acttcggcgg aatggccgtg
     3781 atcacggtaa tcgcgttctc gttcggcgca gcgctgactg cgccgaggca tcgtgcgcta
     3841 ctccttgccg gcggagcgat cggtgccgca tttctcgcga gcgggctcgt cgccatcggc
     3901 tcctacgcgt caggcgtgaa cgcgggcgcc ggaatccatc gggacgcatc ggcgctcatg
     3961 gcctacggtc tgcgcccgat cgagctcgtg gtcccgaccg cgaaccacct cgtgttcaac
     4021 ctcgactcct tctggctacg gcacgaacat ggctcgaacc cgaccgagat cagcaactac
     4081 ctcggcctgc tcacgatcgc actcgcggtc ggctgggtcg tcgtcgtgct tcggcgccgc
     4141 aacggcatgg caaccgtgac ggccggattg atcgccgcgt tcgtcgtcgg cttcctcttt
     4201 gcactgccga gcccggtggc aggaatctcc atgccgtcga agctgctctg gaacgtgctc
     4261 ccggcattcc gcgtgccgtc gcgctgggat ccgctgctga tgacggcgct gcttcctctc
     4321 gcagcgctcg gcttgcagac gctcacgggc acgatcgagc ggcggcgccg cttcgcgtcg
     4381 gtcgcaatcg tcggcgcagc cctgcttctc tcgttcgtcg agctctcgac gaatcggccg
     4441 taccacttcc gtacggttcc tgttcctccg gagtacgcgg caatcgagac gaagacgccg
     4501 aacgggatcc tcgcggagta cccactcggc tactccgaca tctaccgcct ctggcagcgc
     4561 gttcacgagc gcccactcgt gaatggggcg ccggaaggct ctgtcgccga ccaagtccgc
     4621 ctcatgatcc tcgaccccgc gcaggcgggg accgcgcaga cgttgtcgct cctcggagtc
     4681 accgccgtcg ccatccaccc cggcgggccc gcagacacgc ctctacaacc gcgcgagccg
     4741 aggccggccg acggctatcg acttgtcggc cgattcccgg accgctcctc cgtgtgggcc
     4801 gtaaccgcac ctcccgcgac cgcgctcgtc acgttgccgg gcggcttcgc aaccccgcgc
     4861 ctcgttgcgg gcaaggtcgg ctacccgctg gtcgcgccgt caggtgtggc gctgctcgaa
     4921 ctgcgggcga aggaacacgg cgtcatccgt cttgtctttg acgcgacggc accgagcggc
     4981 agccggaact tccggatcca ggactctgta ggagagcacc ccttcccgct gagcggctcg
     5041 atgcatttcg acctgaacgt cgaggtgccg cgtggtgtct cgcagctgct gctgaagatc
     5101 gatcctgcgc ccgcctccga ggcagacgcc atcgtcctct cgcaaccgcg cgccgagccc
     5161 gcttcaggcg cagcggcgtt gcacgcgagc ccgtcctcag ccgaccccgg cttttgagac
     5221 gaagccacgc cgttcgagat cgccgacgat gacgtcggag ggcgacgcaa cagccaacgt
     5281 cgtcgtacgg cgatagcgac cggggtcgtc gaggaagtcc cgcaggacgt ccgcgctcat
     5341 ctgcgacagg acagcgtttt caccgagacc ctcgcgccgc tccgtcttca ctcggtgcac
     5401 gaggcagggc aaatcgaggt agtagcactc ctcctggctg ccaccgctgt cggtgatgac
     5461 gaacgagctg cgtcgctcga cccgcacgaa gtcgaagaag cgcagccgcg ggatgcgcat
     5521 gaacgtcgac gcatcgaaga aacgagtcaa gccgaaacgc tccagtgcgg cactagtcac
     5581 cggatgatcg atgaagagca gcggtgtcgt gcgcgccgca tctgcgagca cctcgacggt
     5641 ctcggtcagc agccgccggt tgttcaggag ctcgaagcgg tgcagcgaga cgacgccgaa
     5701 cgcggccggc agctcgagcg ccggctcagc gtcggtcacc agctcgagac tgtcgcggat
     5761 cgtgttcgag ccggtgtcga cgatgtcgcc gcgacgaaga ttcccggccg cccaagcgcc
     5821 cggggcatag tggatgcggc tgagcgccgt cgccagcttc ctgttgagct cctccggaaa
     5881 aggatgacgc cagtcgtagc tgcgcaggcc cccttcgatg tgcgcaaccg tcgcgcggag
     5941 cgtgcgtccc atcaggctgc cgaggaccgt cgtcatcgtg tcgccgtgga cgaggaccag
     6001 cggcacgtgc cgcagacgcc gtcgcgcggg gacccatgac cgtgccaccg tggcgaccca
     6061 gcctgggatg tccgaattga cacgcaggtc gcgtccctcg gccccgcgcg caagccacat
     6121 gtcgggttgt cgtagtccga actgctcgag gaacgacgga atctgctgta cctgttgcgc
     6181 cgtcgtggcg agcaggtacg ggtgactgcg gtcgtcgagc ctgagcagca ccggcgcgag
     6241 cttgatcagc tcgccggttg tcccgaagac gacgaggatc accggagcag ctcgagaacg
     6301 ccgcgccaca tgccgagccc gtgaccgagc gcgtagagcg gcgtgacggc cgcgagggtt
     6361 cgcacctcgc ccttcgacct accggcccag gcgccgtatg cggctgcagc tgcgccacac
     6421 gcgccgagcg cggcgggaat gagccgcggg cggcgcagca gcgcgacact caacgggaag
     6481 aaggcaacga ccgccggaaa gaagcgcgat tcaggggtgc cgtggccgtc gagaaacaca
     6541 gtcccccggt gaatcgcatg ccgcagaaac gcgtcgaacg tcgtgcgcgg agaatacgtg
     6601 caggcgaact cgggcgagat gtggatccgc tctgccgccg cgatggcacg caagacgggc
     6661 gcgtcgtcgt tggcgagccg gacgtcaccg taatgcgagc ggaatccggc gagcgccgat
     6721 tcgagcagcg cgcgcggcgc gagaaagcac cccgtccctt tcgggtaacg gtcgaactcg
     6781 gcgatcccga agctcgtcgt acgcgggtcg tcgaagtagt cgcgccatgc aagctcggcg
     6841 agcagccgcc agaactcgcc ccaggcgttg tcggcgtcga cgtggacgtg accgttccag
     6901 acgggtgcgg actggagacg ctcatggacg aagcggagcc cgcctggggc gagacgcacg
     6961 cgtgagtcga ggaggaggac gtactcgccg cgcgcgtgcg ccaggcccgc tcggcgcgcc
     7021 tcgaagcggc cgcgattcgg ctgcgcgacg atgcgcacgg cgatcccgcc gtctgcactc
     7081 gccctcgccg cgtcggcagt gccgtcagtc gagccgtcgt ccacgacgac cacctcggca
     7141 tcgaagtcgc tctcgtcgag ggcggcaacg agcgcgcgca tcgtcgccga cagatgcacg
     7201 gcctcgttgt acacaggaat gacgacactc agcgacgctc gctccatggg ctcaaaccgt
     7261 aacgctaggg tccgcgctag atgttggggc gttactggtg ggaggtcgtg cggcggctcg
     7321 accggccggg ctggcggcac ctgctcgtgg ttccgggctc cgtctgggtc agcttcatgt
     7381 accgcgcacc atgtctcgtc tattggcgaa acggcgcgtg gatccaccgc taccgcggcg
     7441 cgaagattcc gcatgcggga ctcggacgcg ccgcgccgcc ggcggttttc accagggacg
     7501 cacgcgacac ggcgttgtac gtctacacgc cggaccaggg cgatgtcgtc ttcgacgtcg
     7561 gcgcaggtat cggcggtgag acgctgctgt tctcccggct cgtcggcgca tccgggcgcg
     7621 tcgtgtcgat cgaggcacac ccgagcacgt acggccgtgt cgttgaactc tgcaacgcga
     7681 acgggctggc caacgtgacg ccgctgcaag tcgcggcggc agagagcgac ggcaccgcga
     7741 ccatcaccga tctcgcgaat cacgtgcgaa acacgactct cgccgctgcg cgcgacggga
     7801 tcgaagttcc cgcgcgccgg ctcgaaacga ttgcgaagtc gttggacatc acgtacatcg
     7861 atctcctgaa agtgaacatc gagggcgcag aggtgtcggc actccgcggc ctcgggccgt
     7921 tgctcgcgaa gacacgctac gtctgcatcg cgtgccacga cttcctcgcg gacgagggag
     7981 gtgcagagac gctgcggaca aaggcagcgg tacgcgagct ccttctggag cacggcttcg
     8041 agctcacttc gcgcgacgag gccgccgcac cttggacgcg tgactacgtg tacggggtga
     8101 acacacggtt ggagccgcat gccgacgatt gagggtcgaa cggcgaccgc gccggcccag
     8161 ctcggcgcac ctcttcaaga cacgagccag ggttctgacc gtgacgggcg taattcggaa
     8221 ggcggcgctc gcctacgcgc gccgcttcga ctggaacgtc ctgttcggcg atgcgctcgc
     8281 cgagctcgac accagcccgc ggacgaatcc gacgagatag acggcctgcg tggcgacgac
     8341 cgcgaatggc tccgcggcac cgacgagcag cgagcggaag cgaaccgccg catggagccc
     8401 gctgagcacg agcgctgcca cgtacgcgag cacgagcgcg atcccag
//
LOCUS       DASVEU010000038         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_27271,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000038 DASVEU010000000
VERSION     DASVEU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27271"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2115
                     /locus_tag="VF225_01355"
     CDS             <1..>2115
                     /locus_tag="VF225_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143930.1"
                     /translation="EEGRRETLCALGNGYFVTRAAAPDSVADGIHYPGTYLAGGYDRL
                     VSEVAGREIENEDLVNLPNWLVLVLRIEDGEWLRPDGVEMLDYRQELDLRQGLLLRTV
                     RLRDSEGRTTRWEERRFVSMRDRHVAGLAVAVTAEDWSGRLTVRSSLDGTVINHGVQR
                     YRELDGRHLEPVATSQPEDDVIFLQSRMVQSRREIALAARTRCHLDGKEVDPLRRTEQ
                     LDGLAAQEMTLELSAGQTLLIEKLVAFYTSSDPAISESGLEARKKVGELGRFDDLLSE
                     HRAAWKHLWEECDLKITTSADAAVVATLRLHVFHLLQTVSYHTIDLDVGVPPRGWHGE
                     AYRGHILWDELFIFPFLNLRIPALTRALLRYRYRRLPDARRAARAAGYRGAMFPWQSG
                     SNGREESQKVHLNPLSGRWIPDNSHRQRHIGAAVAYNVWQYYQITDDHDFMYFYGAEL
                     LLEIARFWASIASYDETDDCFEIRGVMGPDEFHTAYPDRDPSTEGGLDNNAYTNVMAS
                     WTLTRALDALALLPDEHCRRLCETIGLGRDEIEAFDEISRKLRIPFHGDGIISQFEGY
                     ERLQEFDWQGARERHEDLQRLDRLLEAAGDTPNRYQASKQADVLMLFYLFSADELAQI
                     FERLGYDFNPEMIPRNIDYYLERTSHGSTLSWVVHSWVLARADRPHSWQMFQEALRSD
                     VADIQGGTTAEGIHLGAMAGTVD"
BASE COUNT          359 a          722 c          691 g          343 t
ORIGIN      
        1 gaggaggggc gccgcgagac gctgtgcgcg ctcggcaacg ggtacttcgt cacccgtgct
       61 gccgctccgg acagcgtagc cgatggcatc cattatcccg gcacgtacct cgccggcggc
      121 tacgaccgcc tggtcagcga ggtcgccggg cgggagatcg agaacgagga cctcgtcaac
      181 ctgccgaact ggctggtgct ggtgctgcgc atcgaggacg gcgagtggct gcgccccgat
      241 ggcgtcgaga tgctcgacta tcgtcaggag ctcgacctgc gccaggggct gcttctgagg
      301 acggtgcgcc ttcgcgattc cgaaggccgc acgacccgct gggaggagcg gcgcttcgtc
      361 agcatgcggg accggcacgt cgcgggcctc gccgtggcgg tgactgcgga ggattggtcg
      421 gggcgtctca ccgtacgctc ttcgctcgac ggtaccgtga tcaaccacgg cgtacagcgt
      481 taccgcgagc tcgacgggcg gcacctcgaa cccgtggcga cgtcacagcc tgaagacgac
      541 gtgatctttc tgcagagccg gatggtgcag tcgcggcgcg agatcgccct ggccgcgcgc
      601 acgcggtgcc atctggacgg aaaggaggtg gatcctttgc gccggacgga gcagctcgac
      661 ggcctggccg cccaggagat gacgctcgag ctgtccgcgg gtcagacgct cctgatcgaa
      721 aagctcgtcg cgttctacac ttcgagcgac cccgccattt cggaatccgg gctcgaagca
      781 cgcaagaagg tcggtgagct cggtcgcttc gacgacctct tgagcgagca ccgcgccgcc
      841 tggaagcacc tctgggaaga gtgcgacctc aagatcacca cttccgccga cgccgccgtc
      901 gtggccacgc tccgcctgca cgtcttccac ctgctgcaga cggtctccta tcacacgatc
      961 gatctcgatg tcggcgtgcc gccgcgcggc tggcacggcg aggcttaccg cggccacatc
     1021 ctctgggacg agctgttcat ctttccgttc ctgaacctgc gcatcccggc gcttacccgg
     1081 gcgcttttgc gctatcgcta tcggcggctg cccgacgcgc gacgtgcggc cagagccgcc
     1141 ggctaccggg gcgccatgtt cccctggcag agcggcagca atggccgcga ggagagccaa
     1201 aaggtccacc tgaaccccct ctcaggacgc tggattcccg acaactcgca ccgccagcgc
     1261 cacatcgggg cggcggtcgc ttacaacgtc tggcagtatt accagatcac cgacgatcac
     1321 gacttcatgt acttctatgg cgccgagctg ctgctcgaga tcgcaaggtt ctgggccagc
     1381 atcgccagtt acgatgagac ggacgactgc ttcgagatcc gcggcgtcat ggggccggac
     1441 gaattccaca ccgcttaccc ggaccgggat ccgtcgacgg aaggcggcct cgacaacaac
     1501 gcgtatacca acgtcatggc gtcctggacg ctcacccgcg cgctcgacgc gctcgccctt
     1561 ctgccggacg agcactgccg gcgcctttgc gagacgatcg gcctcgggcg cgacgagatc
     1621 gaggccttcg acgagatcag ccgcaagctg cggatcccct ttcacgggga cggcatcatc
     1681 agccagttcg agggctacga gcgccttcag gagttcgact ggcagggcgc gcgcgagcgg
     1741 catgaggacc tccagcgcct cgaccgcctg ctcgaggccg caggcgacac gccgaaccgc
     1801 taccaggcgt cgaagcaggc cgacgtcctg atgctgttct atctgttctc ggcggacgag
     1861 ctcgcccaaa tcttcgagcg tctcggctac gacttcaacc ctgagatgat cccgaggaac
     1921 atcgactact atctcgagcg cacgtcgcac ggctcgacgc tgagctgggt cgtccactca
     1981 tgggtgctcg cccgggccga ccggccgcac tcctggcaga tgttccagga ggcgctgcgg
     2041 agcgacgtcg cggacatcca gggcgggacg acagccgaag gcatccacct cggcgcgatg
     2101 gcgggcacgg tcgac
//
LOCUS       DASVEU010000039         5902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_222876,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000039 DASVEU010000000
VERSION     DASVEU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222876"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF225_01360"
     CDS             <1..379
                     /locus_tag="VF225_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143931.1"
                     /translation="HRREAQRRRREAFARIRAWAILLTHRQPLGITIDGDEVETRLVL
                     VANNAYGLDLPSLGERERLDEGKLHLYSLGADPPERSGERFVVDAAAGRLEAAIDGEP
                     DVLETPMEFWVEPGALRVLVPKA"
     gene            complement(376..870)
                     /locus_tag="VF225_01365"
     CDS             complement(376..870)
                     /locus_tag="VF225_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7143932.1"
                     /translation="MGALDSWRFCPVCGEAIEKIDDRAECHACGYVGYANAVPGAEAV
                     CFDERGRVLLGRRAFDPGEGLWDLPGGFLHENEHPLDGLRREIREETALEIEPIDFLG
                     FWLEPYDGRIVLCLAWTARATGEPRAGDDLVELRWFEPDVLPPPAELAFAHYPDVLSV
                     ACGA"
     gene            916..1293
                     /locus_tag="VF225_01370"
     CDS             916..1293
                     /locus_tag="VF225_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7143933.1"
                     /translation="MSPRAVGATQHELSRIGLLATLSGETLARLAQNMVREDIPAGAG
                     VVSEGEEGDRFYVVLSGMLAVSQRSRGAQSVLRPGDYFGEVALAMDVPRTATVRALTP
                     VTVASCDRETFDEFIRPLFADDS"
     gene            1358..2140
                     /locus_tag="VF225_01375"
     CDS             1358..2140
                     /locus_tag="VF225_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7143934.1"
                     /translation="MSLWHDIQGEGPPVVLLHAGLTDSRLWEPQLRSFPSSHSVLRVD
                     LPGFGNSPLETNPVSFRGAIRDVMDTEGIDRAALVGVSLGGNTALELALESPERVSAL
                     VLVGAGLPDHQWSQEVTAFFADEEAALERGDPDAAVDANLRMWLAGPHRSLDDMDPAT
                     RELVGEMQRQAFRQQKGHDDVRMLRLEPPASVRLGDVSAPTLVLTGDDDVSDIHRIAD
                     RLAAGIPGAERATIAGAAHLPSLDRPEEFDRIVLDFLARHGV"
     gene            complement(2283..3086)
                     /locus_tag="VF225_01380"
     CDS             complement(2283..3086)
                     /locus_tag="VF225_01380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7143935.1"
                     /translation="MEEPLRQIDDWPAPFAAAGVLRGGEVVATHGARNEVLRWASLTK
                     LVTALAALVAAEEGVIDLDEAAGPEGSTVRHLLAHASGLPFEPGAPTGRPGQRRIYSN
                     VGFETLAEHIAERADMPFADYLGAGVLQPLGMQAELRGSPAADLHGSLDDLVILAREL
                     QQPTLLAPETLAEATTVQFPGLAGVLPDFGRFDPNDWGLGFELRDEKSPHWTGTRNSP
                     QTFGHFGGSGTFLWVDPDARFALGVLTNLDFGEWAKEAWPRLSDAVLIA"
     gene            3151..3492
                     /locus_tag="VF225_01385"
     CDS             3151..3492
                     /locus_tag="VF225_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX7143936.1"
                     /translation="MGFSRAVRDGRHVFVAGTCAVMPEGGAPPADAYGQAKRCLEIIA
                     AALAEAGAAAEHVVRTRTFLLDATDWEEVGRAHGELFRHVRPASTMIVVSALLDPRWL
                     VEIEADALLPE"
     gene            3505..4728
                     /locus_tag="VF225_01390"
     CDS             3505..4728
                     /locus_tag="VF225_01390"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX7143937.1"
                     /translation="MEHDGKQIYPASITGKGSSFAPGGSVLDHAFGKGDPYTLGVEEE
                     YMLLDAETFDLVQHIDTVLAAVAGHELEPVINAELMQSVLEIATPVCHTTGEVAEQLR
                     RIRSYVGGIAHEQGLRVGSAGTHPFSLFERQRITARDRYRALVDQMQYVARRELIFGM
                     HIHVAVDDPDKAIQVVNGLLPHIGPLLALSASSPFWRGEPTGLRSSRQMIFAAFPRSG
                     PPPRFKNYAEYAEVVGQLEKTGCIPDYTHIWWDIRLHPRLGTIEVRICDAVTRLEHVV
                     AIVAYCQALVKLFSERFDRGEEIPSYHRILTTENKWLAGRYGLEAPLMDLATGRRNRV
                     AVAQLIRRTLKEIEPHARELGSDRELEGLEEILRNGNGADRQLRVFNANRDIVEVVSE
                     IADATEVAAAPQLAK"
     gene            4741..4875
                     /locus_tag="VF225_01395"
     CDS             4741..4875
                     /locus_tag="VF225_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143938.1"
                     /translation="MLGIGDRIPKATVWTAPHERRSLDELLADGPALFVFYLFDWSST
                     "
     gene            4885..5199
                     /locus_tag="VF225_01400"
     CDS             4885..5199
                     /locus_tag="VF225_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7143939.1"
                     /translation="MELLRDRSPEFLAAAVRPFGISGDSEWTHIAWAQALDLNFPLLS
                     DWNKEAMQAFGIEADFRGHRGVADRSAFLVDQDGTVRGAWRYDTAEVPDFDELLRAAQ
                     AL"
     gene            5133..>5902
                     /locus_tag="VF225_01405"
     CDS             5133..>5902
                     /locus_tag="VF225_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143940.1"
                     /translation="MALRHGRGAGLRRAASRRAGFVALGAALYLAAGATATWPAVQHA
                     RSNFLSGGAPAHGEASPGDHLQTLYHYWLVGHQLEHGRGPWRDPYSFRPEVKPQPNFP
                     GWPYGILFWPLGAVFGLVGGWNVLQLLFYALAGLAACAWLLELGLPRGPALAGGLAFA
                     IAPYRVEQSVGHLLGPISILLPLSLWAFERARRGTRWWLTLAGAALASIPLSGQVHLA
                     LGAIPFFLGYAFCRTRDRRLWLGAGACALAAVGAGLVVR"
BASE COUNT          939 a         2041 c         1998 g          924 t
ORIGIN      
        1 gcatcgccgc gaagcgcagc ggcgacgccg cgaggcgttt gcgcggattc gggcgtgggc
       61 gatcctgctc acgcaccggc agccactagg catcacgatc gacggtgacg aggtcgagac
      121 ccgcctcgtg ctcgtcgcga acaacgcgta cgggctcgac ctgccctcgc tcggcgagcg
      181 cgagcgcctc gacgaaggga aactgcactt gtactccctg ggcgccgatc cgcccgagcg
      241 cagcggagag cggttcgtcg tggacgcggc ggccgggaga ctcgaggcgg cgatcgacgg
      301 cgagccagac gtactcgaga cgccgatgga gttctgggtc gagccgggcg cgctacgtgt
      361 gctcgtgccg aaggcttagg caccgcaggc gactgagagc acgtccgggt agtgggcaaa
      421 cgcgagctct gcaggcggcg gcaggacgtc cggctcgaac cagcggagct ccacgagatc
      481 gtcgcctgcc ctgggctcgc ccgtcgccct ggcagtccac gccagacaga gcacgatccg
      541 cccgtcgtag ggctcgagcc agaacccgag gaagtcgatc ggctcgatct cgagagctgt
      601 ctcctcgcgg atctcgcgcc gcagcccgtc gagcggatgc tcgttctcgt ggagaaatcc
      661 accgggcaga tcccagaggc cttcgccggg atcgaacgcg cgacggccga ggagcacacg
      721 accccgctcg tcgaagcaga ctgcctccgc gccgggaacg gcgttggcgt agcccacgta
      781 accacaggcg tggcactcgg cccggtcgtc gatcttctcg atagcctcgc cgcagaccgg
      841 acagaagcgc caggagtcga gcgcacccat ggaggggacc atgcacgatc agccggagcc
      901 gaccgaggaa gaacagtgag cccgcgggcc gttggggcga cgcaacacga gctgtcgcgg
      961 atcggattgc tcgccacgct ttccggtgag acgcttgcgc ggttggcaca gaacatggtg
     1021 cgcgaagaca ttccggccgg tgccggcgtc gtgtcggagg gcgaagaggg cgaccgtttc
     1081 tacgtcgtgc tcagcggcat gctcgcggtc agtcagagaa gccgcggcgc acagagcgtg
     1141 ttgcggccgg gcgactactt cggcgaggtc gcgctggcga tggacgtgcc gcgcaccgcg
     1201 accgtccgtg cgctcacgcc cgtgacggtc gcgagttgcg atcgggagac gttcgacgaa
     1261 ttcattcgtc cgctttttgc ggacgactca tgatcgtcgc tcgccttcgg ttcgctcccg
     1321 tcgccccctc cacggctcat aggccaagga tcggccgatg agcctctggc acgacatcca
     1381 gggcgaaggc ccgccggtcg tgctcctgca cgcaggcctc actgattcgc gtctctggga
     1441 acctcagctg cgctctttcc catcgtcgca ttcagtgctg cgcgtcgacc tacccggttt
     1501 cggcaactcg ccgctcgaga cgaaccccgt gtcgttcagg ggcgcgatac gcgacgtgat
     1561 ggacaccgaa ggcatcgacc gggccgcgct ggtcggcgtc tcgctcggcg gcaacacggc
     1621 gctcgagctc gcactcgagt cgccggaacg ggtttctgct ctcgtgctcg tcggcgccgg
     1681 gctccccgac caccagtggt cgcaggaggt gacggccttc ttcgccgacg aagaagcggc
     1741 gctcgaacgc ggagacccgg atgctgccgt cgacgcgaat ctgcgcatgt ggcttgccgg
     1801 cccgcaccgc agtctcgacg acatggatcc agcaacgcgc gagctcgtcg gcgagatgca
     1861 aagacaggcc ttcaggcagc agaagggaca cgacgacgtg cggatgctgc gcctcgagcc
     1921 tcccgcgtcg gtgcgactcg gcgacgtgag tgccccgact ctggtgctga cgggcgacga
     1981 cgacgtcagc gatatccacc gcatcgccga tcgtctcgct gccggaattc ccggtgcgga
     2041 gcgcgcgacg atcgccggag cggcgcatct cccgagcctc gaccgcccgg aagaattcga
     2101 ccggatcgtc ctagatttcc tggctcggca cggcgtctga gcaggcgtgc tcggttccga
     2161 cacgtaccgg tgccaggcac gggtacgaaa cgttcaccga agcgtcacgc gagacacgat
     2221 tgccctgcaa gcaagacgag cgtatcggtg ccaggcaccg gtacgaaacg gtgacacgtc
     2281 tgtcacgcga tcagcacggc gtccgacagg cgtggccacg cttccttcgc ccactcgccg
     2341 aagtccaggt tcgtgaggac gccgagcgcg aagcgggcat ccgggtcgac ccagaggaac
     2401 gtaccgctgc caccgaagtg gccgaacgtt tgcggcgagt tgcgcgtgcc ggtccagtgc
     2461 ggcgacttct cgtcgcggag ctcgaaaccg agaccccagt cgttcgggtc gaagcgaccg
     2521 aagtccggta gcacgccggc gaggcccgga aactgcaccg tcgtcgcttc cgccagcgtc
     2581 tccggcgcga ggagcgtggg ctgctgcagc tcgcgggcca ggatcacgag atcgtcgagc
     2641 gagccgtgca gatctgcggc cggcgagccg cgaagttcag cctgcatccc cagcggctgc
     2701 aagacgcccg cgccgaggta gtcggcaaac ggcatgtccg ctcgctcggc gatgtgctcg
     2761 gcgagcgtct cgaagccgac gttcgagtag atacgccgct gcccaggtcg tcctgtcggc
     2821 gcgccgggct cgaagggcag accggaggca tgcgcgagca ggtgtcgcac ggtcgatccc
     2881 tccgggccgg ccgcctcgtc gagatcgatc acgccctcct cggcggcgac gagcgccgct
     2941 agagccgtga cgagcttcgt cagggaagcc cagcgcagta cctcgtttcg ggctccgtgg
     3001 gtcgccacga cttcgccgcc acgcagaaca cccgcagccg caaacggtgc cggccagtcg
     3061 tcgatctgcc tcagtggttc ttccacggcg cgcgatacta gatgcctgga tcgccgcctc
     3121 gtctcctccg gttctcccta cgagcccacg atcggcttct cgcgggcggt gcgcgacggc
     3181 cgccatgtct tcgtcgcagg gacctgcgcg gtcatgccgg agggcggggc cccacccgcc
     3241 gatgcatacg ggcaggcgaa gcgctgcctg gagatcatcg ccgcggcgct cgccgaggct
     3301 ggggccgcag cagagcacgt cgtgcgcacg cgcacgttcc tcctcgacgc cacggactgg
     3361 gaagaagtag gccgcgccca cggggagctt ttccgccacg tgcggcctgc cagcacgatg
     3421 atcgtcgtgt ccgccctcct cgacccgcgg tggctggtcg agatcgaggc tgacgcgctg
     3481 ctccccgagt agtaagactc ccgtatggag cacgacggga agcagatcta cccggcttcg
     3541 atcacgggga agggatcgag tttcgcgccc ggcggcagcg tgctcgacca cgccttcggc
     3601 aagggcgacc cgtacacgct cggagtcgag gaggagtaca tgctgctcga tgccgagaca
     3661 ttcgatctcg tgcagcacat cgacaccgtg ctcgcggcag tggccgggca cgagctcgag
     3721 ccggtgatca acgcggagct gatgcagtcc gtgctcgaga tcgcgacacc ggtctgtcac
     3781 acgaccggcg aggtggcgga gcagctgcgc aggatccgga gctacgtcgg cggcatcgcg
     3841 cacgaacagg gactgcgcgt cggctccgcg gggacccatc cgttcagcct cttcgagcgg
     3901 cagcgaatca ccgcgcgcga tcgctaccgc gcgctcgtcg accagatgca atacgtcgcc
     3961 cgccgcgagc tcatcttcgg gatgcacatc cacgtggctg tcgacgaccc cgacaaggcg
     4021 atccaggtcg tcaacgggct gcttccccac atcgggccgc tgctcgcact gtccgcgagc
     4081 tcgcctttct ggcgcggcga gccgaccggg ctccgctcga gcaggcagat gatcttcgca
     4141 gcgttcccac gctcggggcc gccgccgcgc ttcaagaact acgcggagta cgcggaagtc
     4201 gtcggacagc tcgagaagac cggctgtatc ccggactaca cgcacatctg gtgggacatc
     4261 cgccttcacc cgcgcctcgg cacgatcgag gtgcgcatct gcgacgccgt gacgcgcctc
     4321 gagcacgtgg tcgcgatcgt cgcgtactgc caggcgctcg tgaagctctt ctccgagcgc
     4381 ttcgaccgcg gcgaggagat cccctcctat caccgcatcc tcacgaccga gaacaaatgg
     4441 ctcgcggggc gctacgggct cgaggcgccg ctgatggatc tcgcgacggg ccgccggaac
     4501 cgggtcgcgg tcgcgcagtt gatccggagg acgctgaagg agatcgagcc gcatgcacgc
     4561 gagctcggct ccgaccgtga gctcgagggc ctcgaggaaa tcctgcgcaa cgggaacggc
     4621 gccgatcggc agctcagagt cttcaacgcg aaccgcgaca tcgtcgaggt cgtcagcgag
     4681 atcgcggacg cgacggaggt ggcggctgcg cctcagcttg cgaagtaggg ttcgcgcccg
     4741 atgctgggaa taggggaccg aattccgaag gcaaccgtct ggaccgcacc gcacgagcgg
     4801 cgcagtctcg acgagctgct cgccgacgga ccggcgctct tcgtcttcta cctcttcgac
     4861 tggtcttcca cctgaacgca cgagatggag ctcctgcgtg acaggagccc ggagttcctg
     4921 gcagcggctg tccgcccatt cggcatctcc ggcgactccg agtggaccca catcgcgtgg
     4981 gcccaggcgc tcgacctaaa cttcccgctc ctgtcggact ggaacaagga ggcgatgcag
     5041 gcgttcggca tcgaagcgga cttccgcggc caccgtggcg tcgcggatcg aagcgcgttc
     5101 ctcgtcgacc aggacggtac ggtccgcggc gcatggcgct acgacacggc cgaggtgccg
     5161 gacttcgacg agctgcttcg cgccgcgcag gctttgtagc gctcggcgct gccctgtacc
     5221 tcgcggcagg ggcaaccgcg acgtggccgg cggtccagca cgcccggtcg aactttctct
     5281 cgggtggtgc gccggcccac ggcgaagcgt ctccgggcga ccacctgcag accctctacc
     5341 actactggct cgtcggccat caactcgagc acggccgggg tccttggcgg gacccgtaca
     5401 gcttccgccc cgaggtgaag ccgcagccga acttccccgg ctggccttac gggatcctct
     5461 tctggccgct cggcgcggtg ttcggcctcg tcggtgggtg gaacgtcctg cagctcctct
     5521 tctacgcgct tgccggtctc gcggcgtgtg cctggctgct cgagcttggc ctgccccgcg
     5581 gcccggcact cgcaggcggg ctcgcatttg cgatcgcgcc ctaccgcgtc gagcaaagcg
     5641 tcggccacct gctcggacca atctcgatcc tcctgccgct ttcgctgtgg gccttcgagc
     5701 gagcgcggcg cggaacccgc tggtggctca cgctcgcagg tgcggcgctc gcatcgattc
     5761 cgctctcggg gcaggtccat cttgcgctcg gagcaatccc gttcttcctc ggctacgcgt
     5821 tctgccggac gcgcgaccga cggctctggc tcggtgccgg tgcctgcgcg ctcgcggcgg
     5881 tcggcgcagg cctagtcgtc cg
//
LOCUS       DASVEU010000040        13927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_223030,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000040 DASVEU010000000
VERSION     DASVEU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13927
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223030"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..869)
                     /locus_tag="VF225_01410"
     CDS             complement(87..869)
                     /locus_tag="VF225_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7143941.1"
                     /translation="MAAVQKRDYYEVLELERDANAGAIKRAFHALAREWHPDVSDSPD
                     AGARFRELAEAYTVLSDRQARLLYDRYGYRGRGNQGFDEALWEARPAVAARGENVHVG
                     IELRSFEATEGARQLVSYEALVRCKTCAGRGSVGSPDPECEHCRGTGRKRTVSNLEVA
                     NLLQIEPCPACIAEPCSRCVGEGTVSAERRIRLLVPPGIQDGMQLRVGGDGNDAGAGS
                     IPGDLLVGVKVLAPPRDPRFVRYAALVLLFVAIATLALYVSH"
     gene            1395..2090
                     /locus_tag="VF225_01415"
     CDS             1395..2090
                     /locus_tag="VF225_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143942.1"
                     /translation="MGRFRTSRAWIATSGPKRRRVVTLTFSLPHAERVLFVIEQVAPS
                     CRTVQQFTVRGHAGRNWIRFPAARSRLRLVPGTYRISAHTSGGLLVRRVTIVVVDRGK
                     PSRAEIAAARAANVCPAADRIASAKGSGGASNTSSPAGSPSGTPLHEGANTRSGSVLG
                     ASLTRAARALEPLVIGLLGLAIALLGIASLPRFATTGSPTNEFLDRYRIAIAGLGATA
                     LAAVVVMIALRPG"
     gene            complement(2099..3139)
                     /locus_tag="VF225_01420"
     CDS             complement(2099..3139)
                     /locus_tag="VF225_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143943.1"
                     /translation="MTRRDVIEIGVAAALVAVAAGAHYADATPVLTFVLAALAIAVLA
                     RLVGTSTEQLGSRVGSSAAGAIQSALGNLPELFIALFALHQGLTLVVQAALVGSVIAN
                     SVFVLGLAFIVGGLRNGTQTFDSPRARLIATLTILSAAILSVPTLSHAVHTQAASHEK
                     SLSLICAGVLIALFFLTLPALLSVRDTSEAEPPRWSLPITVTVLGGAGVAAALVSDWF
                     VTALKPATEALGMSQVFAGLIVVAIAGNAVENVVGVQLAARNRPDFAISVIINSALQV
                     ALLLTPILVFASLFFATPLTLVFPTLLAISLLLSAWILAVVVYDGDSTWEEGAILVGF
                     YVVIAASFWWGA"
     gene            3157..3627
                     /locus_tag="VF225_01425"
     CDS             3157..3627
                     /locus_tag="VF225_01425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7143944.1"
                     /translation="MEGEELREVMRRFPAPVAVVTTALEGERFGLTVGSFVSLSLAPP
                     LVGISIGKDSSSHEPIRRAGGWSASLLSEAQANVAQHFARSGIPPVALWNGVDVRDGS
                     RGPLVNEALAWLECRTLSEHNAGDHTIFVGEVESTELGRSGPGLVYRGGEYLPA"
     gene            3624..4301
                     /locus_tag="VF225_01430"
     CDS             3624..4301
                     /locus_tag="VF225_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7143945.1"
                     /translation="MIDAVVFDLDGVIIDSEELWDEVREGLARERGGSWSAQAQADMM
                     GMSSTEWSRYMHEVVGLPESPAEINREVVDQMLARYSEHLPLIDGAVDAVTRLAAHWP
                     LGVASSSNRQLIDRVLEVSGLVPYFKATVSSEEVERGKPAPDVYLEAARRLGVEAARN
                     AAIEDSASGIRSAHAAGMHVVAIPNRAFPPPADVLALADVVLDSIAELGPETIREVAR
                     LWTASSG"
     gene            complement(4261..4647)
                     /locus_tag="VF225_01435"
     CDS             complement(4261..4647)
                     /locus_tag="VF225_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX7143946.1"
                     /translation="MMTSPQLERGPVGNTRSIGLSILWAILTIGIYTFVWTYKTHEEI
                     KRYSGNGVGGVIGFVIYFLLSPVTFFIVPSEVRYMYEDLDGQASPVRGLTGLWILLPL
                     IGNIVWFVKVQGALNRYWQSKGAPPP"
     gene            complement(4644..5288)
                     /locus_tag="VF225_01440"
     CDS             complement(4644..5288)
                     /locus_tag="VF225_01440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7143947.1"
                     /translation="MTFIVDPRVEAYAEEHTTPEGELFERLAAETREKTTAPQMMVGR
                     LEGRFLGVLVSGLRARRVLELGTFTGYSSISMALALPSGGRIISCDVNEETTAIARRY
                     AEEAGVADRIDYRLGPGLETIAQLDGPFDLVFIDADKPNYVNYYEATLPLLADGGLIV
                     VDNTLWSGRVADEEDDEETTRAIRALNDHVSADPRVENVLLTVRDGMNLVWRAR"
     gene            complement(5285..6514)
                     /gene="fabF"
                     /locus_tag="VF225_01445"
     CDS             complement(5285..6514)
                     /gene="fabF"
                     /locus_tag="VF225_01445"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7143948.1"
                     /translation="MRRIGVTGLGAVTPLGNDARSTWNAAVAGESGIDWIRSFDPGDA
                     PVRIAAEVKDFDATQVASTKEVRKLERNVLLAMGAAREAMGDAGLNGFDPKRVGVVFG
                     TAIGGVNGLLEQEQIRRERGPERVSPNFLPNVLVDSASGQLAISLGIKGPNYAVVSAC
                     ATGSHAIGEAAELIKRGDADAMLAGGGEACLNPLILAGFTAMRGLAVENEDPPRASRP
                     FDATRAGFVMGEGACVFVLEELEAARARGAQVYAEVLGYGASNDAHHMAQPEPEATGV
                     ADMMRAALHRAGIEPERVGYINAHGTSTPLGDLAETKAIKDVFGDHAYNLAVSSTKSM
                     MGHTFGAAGAIEAMMCVLALHEGVIPPTINYRQPDPDCDLDYVPNEAREAKVDVALSN
                     AMGLGGHNGCVLLGRVE"
     gene            6642..6944
                     /locus_tag="VF225_01450"
     CDS             6642..6944
                     /locus_tag="VF225_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7143949.1"
                     /translation="MTVAIPSLAEQPRLVFFHSALSGHCRRVEGFLAQVLQRRRNHGT
                     FDVVKVAEEEHPDLLERFRIETVPTLVVVEHNAVAARLERPRGCREIEGFLAPWLN"
     gene            complement(7001..7492)
                     /locus_tag="VF225_01455"
     CDS             complement(7001..7492)
                     /locus_tag="VF225_01455"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX7143950.1"
                     /translation="MGQLKEELVKHIDEAHAMEQNVLRMLDSMIATTDDPEILDALEH
                     HKVQTQGHADRMAQRLEAHDLSPSAVRQLGGVLGALAKMPLDFVRGENAGRNARDAYA
                     TEHLEIASYELLRRIAQRAGDEETATAALEIIEEERAMALIIEQNWDRFAELSLQEEG
                     VIV"
     gene            7612..8721
                     /locus_tag="VF225_01460"
     CDS             7612..8721
                     /locus_tag="VF225_01460"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7143951.1"
                     /translation="MPFTHPSSGGNAPETRSTAETPSSSPTSVGFRAVPGPNVPFPPM
                     EAQLVKELPDTHGWQYEPKWDGFRGVLENDGGEVALWSRNGRPLLRYFPELRPLGDLL
                     PPHSALDGEIVIARDGVLDFDSMQMRLHPAESRVRKLAAEIPADFIVFDLLLWKGKPV
                     HELPLEERRAELERVLADDFHLSPTTGDAADAQKWLDRFEAAGLDGVIAKKLGLPYLP
                     GSRDGVLKVKPYKTADCVIVGVRWKEKPTRIATLLLGLYGKGGKIDYVGSAAVAAGRH
                     KEIFDRIEPLLKDAPDRGFSEPNRWGTGELQESAVRPQLVAEVRYDKVQGNRFRHGSR
                     FIRFRDDKDPEQCTWREVRPPRRKDDPTFESLLAG"
     gene            complement(8718..9611)
                     /gene="ligD"
                     /locus_tag="VF225_01465"
     CDS             complement(8718..9611)
                     /gene="ligD"
                     /locus_tag="VF225_01465"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX7143952.1"
                     /translation="MDVGGHEVSITNPGKIFFPEPGLTKGDLAEYYVDLADAAVPHLQ
                     RRPFHMVRYPNGVEGKFFHQKRVPPHPEYVGEQYVEFPSGHSTVFAVIDNAAALAWVV
                     NLGCIELHTWHSRVDDIERPDYLLIDLDPTTDGQWPYVREIALVVREVMDELGLASYP
                     KTSGATGLHIMAPIEAELLFPDVRRLAKALAEEVERRVGDQEVATTTWRVADRRGVFV
                     DFGQNTRDRTIATAYSVRPTPDARVSAPLLWDEVAAVEPAAFTVETMRDRVADVGDLT
                     ADMWEHKVSLGPCFEKLGLVL"
     gene            9645..10523
                     /locus_tag="VF225_01470"
     CDS             9645..10523
                     /locus_tag="VF225_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7143953.1"
                     /translation="MRRAPAILLVVLLSILAAGCGGSSNNASDTTSTAAAASCDKGSL
                     NLVNAGQLTVGTDNPAFPPWFGGAEKAPWKVSNPYSGQGYESAVAYAVATKLGFAKSE
                     VKWIVVPFNTSFAPGAKKFDFDINQISYKPARATAVDFSDSYYNVNQSLVVVKGTPIA
                     SATSVAALKPYKFGAQLGTTSYDYIINNIKPSKKPAVFDTNDAAVAALKNKQIDGLVV
                     DLPTAFYITAVQVPKGKILGQFPTVGTPEHFGMIFEKGNSLVSCVNDALTKLRTDGTL
                     GSLQAKWLQADAPVLK"
     gene            10564..11370
                     /locus_tag="VF225_01475"
     CDS             10564..11370
                     /locus_tag="VF225_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7143954.1"
                     /translation="MTSAGEGRRGVTVALLSTVVFFTLLAVAITHAPGWPEVKRTFFN
                     WNQFRDSMPEISRAFLLNVKIFMIAEAFILVLALVLAVLRSLPGPVFFPMRALGIAYA
                     DFFRGVPTILVIALLGFGAPALQLSWVPLSTTFWGIVALILVYTAYVSEVYRAGIESV
                     HPSQDAAARSLGLTHWQSLRKVVLPQAVRRVIPPLLNDFIGLQKDTALVGFLGSVEAF
                     KQSQIDAAATFNYTPFVAAAVLFVALTIPLARFTDWLVLRDRRRRQATGT"
     gene            11370..12116
                     /locus_tag="VF225_01480"
     CDS             11370..12116
                     /locus_tag="VF225_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7143955.1"
                     /translation="MSEALVVDGVHKSFGQLEVLRGVDLAVAEHEVVCLIGASGSGKS
                     TLLRCVNLLEPIDEGRILVEGDEITGRGVDVNRVRRRIGIVFQAYNLFPHMSVLVNVT
                     LAPMLALRIPRAQAEAEATTLLERFGLADKRNDYPDRLSGGQQQRVAIVRALAMKPDL
                     MLLDEVTSALDPELVAEVLEVIRELAAAGMTMLIATHEMSFARDIADRVCFLDEGVIL
                     EQGPPAQILSEPKEPRTQQFLQRIIEAGRL"
     gene            12277..12570
                     /locus_tag="VF225_01485"
     CDS             12277..12570
                     /locus_tag="VF225_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7143956.1"
                     /translation="MQERGTRGIDLSIEPLDDYVVIQPSDEESETNLGLILPASAEAD
                     CRTGVVTAVGAEATGVEPGDKVLFPSDAGYEVRLAGTAVRVMRRGDLIARVHD"
     gene            complement(12572..13588)
                     /locus_tag="VF225_01490"
     CDS             complement(12572..13588)
                     /locus_tag="VF225_01490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX7143957.1"
                     /translation="MSGPVNVWDYEQLAERKLDLNAHAYFAGGAGDEVTLRDNLAAFE
                     RRKLRPRVLVDVSSISTATSILGTDVALPILIAPLAMQRMAHPDGELATARAAAAAGT
                     IMCLSTAATARPAEVAAAAPGAPRWFQVYVFADRSETEVLIAEAVESAFSALVLTVDT
                     PYLGRRERDLRIDFKVPEHLTVTGDLFGGHFDTGLSWHDLEWLAGYSLPVVVKGILTA
                     EDARLACEHGAAAVVVSNHGGRQLDGVSSSLDALEEVVDTVDGRAEVLLDGGVRRGTD
                     VLKALALGARGVLIGRAMVWGLAVAGEDGVMHVLQLLREEVELGLALLGCASPAEVTR
                     SHAT"
     gene            complement(13714..13802)
                     /locus_tag="VF225_01495"
     tRNA            complement(13714..13802)
                     /locus_tag="VF225_01495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13766..13768),aa:Ser,seq:gga)
     gene            complement(13814..13904)
                     /locus_tag="VF225_01500"
     tRNA            complement(13814..13904)
                     /locus_tag="VF225_01500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13868..13870),aa:Ser,seq:gct)
BASE COUNT         2312 a         4748 c         4591 g         2276 t
ORIGIN      
        1 gagcggcggc cgcagcaggc accgttccag gcaaagccta gcgccaaaaa cgccgatttg
       61 gggcgaaatc cctcaactga gcgggctcag tgagatacgt agagcgcgag cgtcgcgatc
      121 gcgacaaaga gcagtacgag cgccgcgtag cggacgaacc ggggatcccg cgggggcgcg
      181 aggaccttga cgcccacaag caggtcgcct gggatggagc cggcgcccgc gtcgttgcca
      241 tcaccgccga cccgcagctg catgccgtcc tgtattcccg gcggcacgag cagacggatc
      301 ctccgttcgg ccgagaccgt cccttctccg acgcagcgcg agcagggctc ggcgatgcaa
      361 gccgggcacg gctcgatctg caaaaggttc gccacttcaa ggttcgacac cgtgcgcttg
      421 cgtcccgtgc cgcggcagtg ctcgcattcg gggtcgggcg aaccgaccga gccccgcccc
      481 gcgcaggtct tgcagcggac gagagcctcg tagctgacga gctggcgcgc cccctccgtg
      541 gcctcaaaac ttcgcagctc gattccgacg tggacgtttt ctccccgcgc tgccactgca
      601 gggcgcgcct cccagagtgc ctcgtcgaag ccctggtttc cgcgcccccg gtagccgtag
      661 cggtcgtaga ggagccgcgc ctgccgatcg gagaggaccg tgtaagcctc tgcaagctcg
      721 cggaaccggg ctccggcgtc cggggagtcg gagacatccg ggtgccattc ccttgcaaga
      781 gcatggaatg cgcgcttgat cgcgccggcg ttcgcgtccc gctcgagctc cagcacctcg
      841 tagtaatccc tcttctggac ggcggccatt cccgagcatt ctcgcgcacc acgcagacac
      901 cgcaaccaat tcggagcctc cggagttgtt taggtatgag ggccgcggcg gccacagtcc
      961 tcctgttggg agccggccta ctggccggga aggcgctcgg ggacacggtt cccgtgcctc
     1021 ccctgcccac cgtgacggtt tcgaccacat ccgtcaccgt gccgatcacg gtccccaccc
     1081 tgccgccacc gccggttaca ttgccgccgc tcacaacgac ctcggtcaag gttccacccc
     1141 cggcgccagc ggcccaggtc acgacgtcga cgactgccgt tgtgccccca ctcccctcgg
     1201 cgtcgtcggt cgtgtccccg acgccggcag gaagctcggg cggagcgtcc tcctcaggag
     1261 ggagctcttc ctcggccggt ggctcatcga cgtccggtag ctcttcatcg gccggtggct
     1321 cgtcgacggc cggtagctcc tcttcgcccg tcaccgactc gtccagttcc gggcccgggt
     1381 ccgacaggcc gaacgtggga cgctttcgaa cctcgcgtgc gtggatcgcg acgagtgggc
     1441 cgaagcgtcg ccgcgtcgta actctcacgt tcagcctgcc gcatgccgag cgggtgctct
     1501 tcgtcatcga gcaggtggcg cccagctgcc gaaccgtcca gcagttcacc gtgcgcgggc
     1561 atgccggccg aaactggatt cgcttcccgg ccgcgcgcag ccggctccgg ctcgttccag
     1621 gcacttaccg aatctcggcg cacacgagcg gaggtctgct cgtccggcgc gtcacgatcg
     1681 tggtcgtcga ccgcggcaag ccgagccgcg cagagatcgc ggccgcccgg gccgccaatg
     1741 tctgcccggc ggccgaccgg atcgcctcgg cgaaggggtc gggcggagcg tcgaacacca
     1801 gcagccccgc aggctcgccc tcggggacgc ccctgcacga aggcgcgaac acccgctccg
     1861 gctccgtgct cggggcgagc ctgacgaggg ccgcccgggc gctcgagccg cttgtgatcg
     1921 gcttgctggg cctcgcgatc gcgctgctcg gaattgcgtc actgccccgc tttgcgacga
     1981 cgggctcacc gacgaacgag tttttggaca gataccgaat tgcgatcgcg ggactgggag
     2041 cgaccgcctt ggctgctgtc gtggtcatga tcgcgctccg ccccggctga cctgctcttc
     2101 acgcgcccca ccagaagctc gctgcaatga cgacgtagaa gccgacgagg attgcaccct
     2161 cctcccacgt gctgtcgccg tcgtaaacga cgacagccag gatccaggca gacagcaaca
     2221 gggagatcgc gagcagcgtg ggaaagacga gcgtcagcgg agtcgcgaag aagaggcttg
     2281 cgaagacaag gatgggtgtg agcagcaggg cgacctgcag cgccgaattg atgatcaccg
     2341 agatcgcgaa gtccggtcgg ttcctcgccg cgagctggac cccgacgacg ttctcgaccg
     2401 cattacccgc gatggcgacg acgatcagtc ccgcaaagac ctggctcatg cccagcgcct
     2461 ccgtcgccgg cttcagcgca gtgacgaacc aatcgctgac cagagctgcc gcaacaccgg
     2521 ccccgcccag gaccgtcacc gtgatcggaa gcgaccagcg cggaggctcg gcctcgcttg
     2581 tgtcgcggac tgacagcagg gccgggagcg tcaggaagaa cagcgcgatc aggacgcccg
     2641 cgcagatgag gctcagggac ttctcgtggg acgccgcctg cgtgtggacg gcgtgcgaga
     2701 gcgtcggcac cgagaggatc gcggcggaca ggattgtcag cgtggcgatc agccgcgcgc
     2761 gcggggagtc gaacgtctgc gtaccgttcc ggagccctcc gacgatgaac gcgagtccga
     2821 gcacgaacac gctgttggcg atcacggacc cgaccagcgc cgcctgaacg accagcgtca
     2881 gcccttgatg cagggcgaac agcgcgatga agagttccgg gagattgccc agtgcagatt
     2941 ggatcgcgcc ggcggcagag gatccgactc ggctgccgag ctgctccgtc gaggtaccga
     3001 cgagccgggc gagcaccgcg atcgcgagtg ccgcgaggac gaaggtcagc accggcgtcg
     3061 cgtcggcgta gtgggcgccg gcagccacgg ccacgagcgc tgccgcgacg ccgatttcga
     3121 tgacgtccct cctcgtcacg tccctatcat cgaggcatgg agggcgaaga gctgcgggaa
     3181 gtgatgcgcc gctttccggc gccggtcgcc gtcgtcacga cggcgcttga aggagagcgg
     3241 ttcgggctga cggtcggctc gtttgtttcg ttgtcgctgg cgccaccgct cgtcggcatc
     3301 tcgatcggca aggactcctc ttcgcacgag ccgatccggc gggcaggagg ctggtcggcg
     3361 agcctgctgt cggaagcgca ggcgaacgtc gcccagcatt tcgctcgcag tggaattccg
     3421 cccgtggcgc tgtggaacgg cgtcgacgtg cgggacggct ctcgcgggcc gctcgtgaac
     3481 gaggccctcg cttggctcga gtgccggacg ctatccgagc acaacgccgg cgaccacacg
     3541 atcttcgtcg gcgaagtcga gtcgaccgag ctgggccggt ccggcccggg cctcgtgtac
     3601 cgaggcggcg agtacctccc agcgtgatcg acgccgttgt cttcgatctc gacggcgtca
     3661 tcatcgacag cgaggaactg tgggacgagg tgcgcgaagg tctcgcgcgt gaacgtggcg
     3721 gcagctggag cgcacaggct caggccgaca tgatggggat gagctcgacc gaatggtcgc
     3781 gctacatgca cgaggtggtc ggactgcctg aatcgcccgc ggagatcaac cgcgaagtcg
     3841 tcgaccagat gctcgcgcgc tattcggagc acctcccgtt gatcgacggg gccgtcgacg
     3901 ccgtcacgcg gctggccgct cattggccgc tcggcgtcgc ctcgtcgtcg aaccgccagt
     3961 tgatcgaccg cgtgctcgag gtctccggcc tcgtgccgta cttcaaggct acggtctcgt
     4021 cggaggaggt cgaacgcggc aagccggcac ccgatgtgta cctcgaggca gcacgccggc
     4081 tcggcgtgga ggcggcgcgc aacgcggcga tcgaggattc ggccagcggg attcgttcgg
     4141 ctcatgccgc tggtatgcac gtcgtcgcga ttccgaatcg tgcgttcccg cctccggccg
     4201 atgtgctggc gctggcggac gtcgtgctcg actcgatcgc cgagctcggc ccggaaacaa
     4261 ttagggaggt ggcgcgcctt tggactgcca gtagcggttg agcgcgccct gcaccttcac
     4321 gaaccaaacg atgttcccga tcagcggcag caggatccac aagccggtta gtccgcgaac
     4381 ggggctcgcc tgcccatcga gatcctcgta catgtagcgc acctcggatg ggacgatgaa
     4441 gaaggtcacc ggcgagagca gaaagtagat gacgaacccg atcacaccgc cgacgccgtt
     4501 ccccgagtag cgtttgatct cctcgtgcgt cttgtacgtc caaacgaacg tgtagatgcc
     4561 gatggtcagg atggcccaga gaatcgacag tccaatcgag cgggtattgc cgacgggacc
     4621 gcgctctagc tgtggtgaag tcatcatcgg gcccgccaga cgaggttcat cccgtcgcgc
     4681 acggtgagta gcacgttctc gacacgagga tccgccgaca cgtggtcgtt gagggcgcga
     4741 atagcccgcg tcgtctcctc gtcgtcttct tcatcagcca cgcggccgga ccagagcgtg
     4801 ttgtcgacga cgatcagccc gccgtcggcg agcaacggca aagtcgcctc gtagtagttg
     4861 acgtagttcg gcttgtccgc gtcgatgaag acgaggtcga acgggccgtc gagttgtgcg
     4921 attgtctcga ggccgggacc gaggcggtag tcgatgcgat ccgcgacgcc ggcttcttcc
     4981 gcgtagcgcc gcgcgatcgc ggtcgtctct tcgttgacgt cacagctgat gatccggccc
     5041 cctgacggaa gcgcgagcgc catcgagatc gacgagtagc cggtgaacgt tccgagctcg
     5101 agcacgcgtc gcgcgcgcag gccgctgacg agaactccga ggaaacgtcc ctcgagacgg
     5161 ccgaccatca tctgtggcgc agtcgtcttc tcgcgggtct ccgccgcgag ccgctcgaac
     5221 agctcgccct ccggagtggt gtgctcctcc gcgtacgcct cgacccgcgg atcgacgatg
     5281 aaggtcactc gacgcgcccc agcaggacgc aaccgttatg gccgccgagc cccatcgcgt
     5341 tcgagagggc gacgtcgacc ttcgcctcgc gcgcttcgtt cggcacgtag tcgagatcgc
     5401 agtcgggatc gggttggcgg tagttgatcg tgggcgggat cactccttcg tgtagcgcga
     5461 ggacgcacat catcgcctcg atcgctcccg cggcgccgaa cgtgtggccc atcatcgact
     5521 tggtcgacga gaccgccagg ttgtaggcgt ggtcgccgaa gacgtccttg atcgccttcg
     5581 tctccgcgag atccccgagc ggggtcgacg tgccgtgtgc gttgatgtac ccgacgcgct
     5641 cgggctcgat tccggctcgg tggagcgcgg cacgcatcat gtcggcgacg ccggtcgcct
     5701 cgggctcggg ctgcgccatg tgatgcgcgt cgttggatgc tccgtagccg agcacctccg
     5761 catacacctg cgctccgcga gcccgggccg cctccagctc ctccaggacg aacacgcagg
     5821 caccttcccc catcacgaag cccgcgcgcg tagcgtcgaa cggacgcgac gcgcgaggcg
     5881 gatcctcgtt ctcgaccgca agcccgcgca tggccgtgaa gccggcgagg atcagcgggt
     5941 tcaggcaggc ctcgccgccg ccggcgagca tcgcgtcggc gtcgccgcgt ttgatcagct
     6001 ccgcagcctc gccgatcgca tgggagccgg tcgcgcacgc ggagacgacc gcgtagttcg
     6061 ggcccttgat cccgagcgag atcgcgagct gcccgctcgc cgaatcgacc aggacgttcg
     6121 gcagaaagtt cggtgacacc cgttcaggcc cgcgctcgcg gcggatctgc tcctgctcga
     6181 gcagcccgtt cacgcctccg atcgccgtgc cgaagaccac gccgacccgc ttgggatcga
     6241 agccgttcag ccccgcatcc cccatcgcct cgcgggccgc ccccatggcc agaaggacgt
     6301 tccgctcgag cttgcgcacc tctttcgtcg aggcgacctg cgttgcgtcg aagtccttga
     6361 cctccgcggc gatccgcacc ggcgcgtcgc cagggtcgaa cgaccggatc cagtcgatac
     6421 cgctctcgcc cgccacggct gcgttccagg tcgaccgcgc gtcgttgcca agcggcgtga
     6481 cggccccgag ccctgtgacc ccgatccttc tcatcgcttg agcttagaca ggcgtgtccg
     6541 gtccgaggtt acgaaacagc agcttcgggg cataaatccg ctcgttccta gcaagtgcaa
     6601 gggccaagtc ctcgcccttc cagattcaac ggaggtgcga catgacagtg gctatcccct
     6661 cgctggctga gcagccgagg ctggtgttct tccattcggc gctgtcgggt cactgccggc
     6721 gcgtggaagg ttttctcgcc caggtgctgc aacggcggcg caaccacgga acgttcgacg
     6781 tcgtcaaggt cgcagaggag gagcatcccg atctgctcga acgattcagg atcgagacgg
     6841 tgccgaccct cgtcgtcgtc gagcacaacg ccgtcgcggc tcgcctcgag cggccccggg
     6901 gctgccgcga gatcgagggc ttcctcgcgc cctggctgaa ctagtccgaa acggccgcgg
     6961 cctggtgtct gacaccaggc cgtggctgtt cgagacgtga ctagacgatg acgccttctt
     7021 cctggaggga aagctcggcg aacctgtccc agttctgttc gatgatcaac gccatcgcgc
     7081 gctcttcctc gatgatctcc agggccgccg tggcagtctc ctcgtcgccc gccctctggg
     7141 cgatgcggcg cagaagctca tagctcgcga tctccaggtg ctcggtcgcg taggcatcgc
     7201 gcgcgttgcg gccggcgttc tcgccgcgga cgaagtcaag cggcatcttc gcgagcgctc
     7261 cgagcacgcc gcccagctgc ctcactgcgg acggcgagag atcgtgcgcc tcgaggcgct
     7321 gcgccatccg gtccgcatgt ccctgcgtct gcaccttgtg atgctccaag gcgtcgagga
     7381 tttccggatc atcggtggtg gcgatcatcg agtcgagcat gcgcagcacg ttctgctcca
     7441 tcgcgtgcgc ctcgtcgatg tgcttgacca gctcctcctt caactgtccc atcccgtcca
     7501 acctcctcat cctgaacaaa aagccccaac ggataatgga tgtcccggcc tcgtacctcg
     7561 aacggaactc ccggaacgtg cggaaactcc gtcgtcccgc gcgagccgtg gatgcccttt
     7621 acgcatccgt ccagcggcgg gaacgcgccc gaaacgcgct cgacagccga gaccccgtcc
     7681 agctcaccca cgtcagtagg gttccgcgcg gtgcctggac caaacgtccc ctttccgccc
     7741 atggaggctc agctcgtgaa agagcttccc gacacgcacg gctggcagta cgagccgaaa
     7801 tgggacggct tcagaggcgt cctcgagaac gacggcggcg aggtcgccct ctggtcgcgg
     7861 aatggccggc cgctgctccg ctatttcccc gagctgcgac ccctgggaga cctcttgcct
     7921 ccccactccg ctctggacgg cgagatcgtg atcgcgcgcg acggcgtgct cgacttcgac
     7981 tccatgcaaa tgcggctgca tcctgcggag tcgcgggtgc gcaagctggc cgctgagatc
     8041 ccggccgact tcatcgtctt cgacctgctg ctctggaaag gcaagccggt ccacgagctg
     8101 ccgctcgaag agcgccgggc ggagctggag cgagtgctcg ccgacgactt ccacctttcg
     8161 ccgacgaccg gcgacgcggc ggacgcgcag aaatggctgg acaggttcga ggccgccggc
     8221 ctcgacggcg tcatcgcaaa gaagctcgga ctgccgtatc tacccggctc gcgcgacggc
     8281 gtgctcaagg tgaagccgta caagaccgcg gactgcgtga tcgtcggcgt gcgctggaag
     8341 gagaagccga cgcggatcgc gacgctgctc ctcgggctgt acggcaaggg cggcaagatc
     8401 gactacgtcg ggtcggccgc cgtcgcggcg ggccggcaca aggagatctt cgatcgcatc
     8461 gagcctttgt tgaaggacgc gccggaccgt ggcttctcgg agcccaaccg ctggggaaca
     8521 ggagagctgc aggagtcggc ggtgcgcccg cagctcgtcg ccgaggtgcg ctacgacaag
     8581 gtgcagggca accgcttccg acacggctcg cgcttcatcc gcttccgcga cgacaaggat
     8641 ccggaacagt gcacgtggcg cgaggtcagg ccaccgcgcc gcaaggacga tccgaccttc
     8701 gagagtctgc tcgcgggcta gagcaccaac ccgagcttct cgaagcacgg cccgagcgaa
     8761 accttgtgct cccacatgtc ggcggtcaga tcaccgacgt cggcgacacg gtctcgcatc
     8821 gtctcgaccg tgaaggctgc gggctcgacc gccgcgacct cgtcccagag gagcggcgcc
     8881 gaaacccgcg cgtccggagt cgggcgcacc gagtacgcgg tcgcgatcgt gcggtcgcgc
     8941 gtgttctggc cgaagtcgac gaagacgccg cggcgatcgg cgacgcgcca cgtggtagtc
     9001 gcgacctcct ggtcgccgac gcggcgctcg acctcctccg cgagcgcctt cgcaaggcgg
     9061 cgcacgtccg ggaagagcag ctccgcctcg atcggggcca tgatgtgcag cccggtcgcg
     9121 cccgacgtct ttggatacga ggcgaggccg agctcgtcca tcacctcgcg aacgacgagc
     9181 gcgatctccc ggacgtacgg ccactgcccg tccgtcgtcg ggtcgaggtc gatcagcagg
     9241 tagtcgggcc gctcgatgtc gtccacgcgc gagtgccagg tgtgcagttc gatgcagccg
     9301 aggttgacga cccacgccag cgcggccgcg ttgtcgatca ccgcgaagac agtggagtgg
     9361 ccgctcggaa actcgacgta ctgctcaccg acgtactccg gatgcggcgg cacgcgtttc
     9421 tggtggaaga acttcccctc caccccgttc gggtagcgca ccatgtggaa cggccgccgc
     9481 tgcagatgcg gcaccgcggc gtccgcgagg tcgacgtagt actcggcgag gtcgcccttt
     9541 gtcagcccgg gctccggaaa gaagatcttg cccgggttcg taatcgagac ttcgtggccg
     9601 ccaacgtcca tggagcgaag tatcggcgtg ttagggtcgc cctcgtgcgc cgagcccccg
     9661 cgatcctcct tgtcgtactg ctctcgatcc tcgccgcagg ctgtggcggc tcgagcaaca
     9721 acgcctcgga cacaacgtcg acggccgcag cggcatcctg cgacaagggc agcctcaatc
     9781 tggtcaatgc cggccagttg acggttggca cagacaaccc ggccttcccg ccctggttcg
     9841 gcggcgcaga gaaggcaccc tggaaggtga gcaaccccta ctccggtcag gggtacgaga
     9901 gcgccgtcgc ctatgcggtc gcgaccaagc tcggcttcgc caagagcgag gtgaagtgga
     9961 tcgtggttcc gttcaacacc tcgttcgcac cgggcgcgaa gaagttcgac ttcgacatca
    10021 accagatctc gtacaaaccg gcgcgggcaa ctgcggtcga cttcagcgac tcgtattaca
    10081 acgtcaacca gtccctcgtc gtcgtcaagg gaacgccgat tgcgagcgca acctcggtcg
    10141 ccgcgctcaa gccgtacaag ttcggcgcgc agctagggac gacgagttac gactacatca
    10201 tcaacaacat caagccctcg aagaagccgg ccgtcttcga cacgaacgac gccgcggttg
    10261 ccgccctgaa gaacaagcag atcgacgggc tggtcgtcga cctccccacg gccttctaca
    10321 tcacggccgt gcaggtcccg aaaggcaaga tcctcggcca gttcccgacg gttggaacgc
    10381 cggagcactt cggcatgatc ttcgagaagg ggaactcgct cgtcagctgc gtcaacgacg
    10441 cgctgacaaa gctccgtacc gacggcacgc tcgggagcct gcaggccaaa tggctccagg
    10501 ccgacgcgcc cgtgctgaaa tagccgtctg aacgctaacc gcggctcgaa gattcttggc
    10561 agcgtgacgt cggccggcga aggccgtcgc ggcgtcacgg tggccctgct cagcactgtg
    10621 gtcttcttca ccttgctggc ggtagcgatc acgcacgcgc ccggctggcc cgaggtgaag
    10681 cgcacgttct tcaactggaa ccagttcagg gactcgatgc cagagatctc gcgcgcgttc
    10741 ctgctgaacg tgaagatctt catgatcgcc gaggcgttca tcctcgtcct cgcgctcgtc
    10801 ctggcggtcc ttcgcagcct ccccgggccg gtcttcttcc cgatgcgggc gctcggcatc
    10861 gcctacgcgg acttctttcg aggcgtgccg acgatcctcg tcatcgcgct gctcgggttc
    10921 ggcgcacctg cgctgcagct gagctgggta ccgctctcga cgacgttctg gggcatcgtc
    10981 gccctcatcc tggtctacac cgcctacgtc tcggaggtct accgcgccgg gatcgaatcg
    11041 gtgcatccga gccaggacgc cgccgcgcgc tcgctcggcc tgacgcactg gcagtcactg
    11101 cgcaaggtcg tcctgccgca ggcggtgcgc agggtgatcc cgccactcct caacgatttc
    11161 atcggcctgc agaaggacac ggcgctcgtg ggcttcctcg gctcggtgga ggcgttcaag
    11221 caatcgcaga tcgatgcggc ggcgactttc aattacaccc cttttgtggc ggcggccgtg
    11281 ctgttcgtcg ccctgacgat cccgctggcc cgcttcacgg actggctcgt gctccgtgac
    11341 cgccgccgac ggcaggcgac agggacctga tgagcgaggc actggtcgtg gacggcgtgc
    11401 acaagtcgtt cgggcaactg gaggtgttgc gcggggtcga cctggcggtc gcggagcacg
    11461 aggtcgtctg cctgatcggc gcatccggct cggggaagtc gacgctgctg cgctgtgtca
    11521 acctgctcga gccgatcgac gaaggacgga tcctcgtgga gggcgacgag atcacgggtc
    11581 gcggagtcga cgtcaatcgg gtgcgccgcc ggatcgggat cgtcttccag gcgtacaacc
    11641 tcttcccgca catgagcgtg ctcgtcaacg tcacgctcgc accgatgctc gcgctgcgga
    11701 ttccgcgcgc gcaggccgag gcggaggcaa ccacgttgct cgaacgcttc gggctcgccg
    11761 acaagcgcaa cgactatccg gaccgcctgt cgggcggaca gcagcaacgg gtcgcaatcg
    11821 tccgagcgct ggcgatgaag cccgacctga tgctgctcga cgaagtgacc agcgcgctcg
    11881 atccggagct cgtcgccgag gtgctcgagg tgatccggga gctcgcagca gccggaatga
    11941 cgatgctgat cgccacacac gagatgagct tcgcgcgcga catcgccgac cgggtctgct
    12001 tcctggacga aggcgtgatc ctcgagcagg ggccgcccgc acagatcctg agcgagccca
    12061 aggagccgag gacacagcag ttcctccaac gcatcatcga ggcaggtcgc ctctgacgtg
    12121 ggggaaccca tggttccccc acgagccccc tccttccgtt ggtgacgagg cggctgatgt
    12181 tgtgcgctgc ggcgtgagcg cggtcacgcc tccgcgcggc ggccggctgt ggcccgacca
    12241 ggttgagcgc cgctcttcgc gctaccgttc gtgctgatgc aggagcgtgg aacccgcgga
    12301 atcgacctct ccatagaacc gctcgatgac tacgtcgtga ttcagccttc cgacgaagag
    12361 tccgagacga accttggact aattctgccg gcgtccgccg aggcggactg ccgcacaggg
    12421 gtggtcaccg ccgtgggcgc cgaggctacc ggggtcgagc ccggcgacaa ggtgctcttc
    12481 ccgagtgacg cgggctacga ggtgcgtctc gcgggaacgg ccgtgcgggt catgcgccgc
    12541 ggcgacttga tcgcccgcgt gcacgactga cttacgtcgc gtgcgagcgc gtcacttcgg
    12601 caggcgacgc gcagccgagc agagcgagcc ctagctcgac ctcctcgcgc aacaactgca
    12661 ggacgtgcat gacgccatcc tcgccggcca cggccaatcc ccagaccatc gctcggccga
    12721 tcagaactcc gcgtgcgccg agcgccaatg ctttgagcac gtccgtgccg cggcgcacgc
    12781 cgccgtcgag cagcacctcg gcgcgaccgt cgaccgtgtc gacgacctcc tccaaagcgt
    12841 cgagcgagct ggacacgccg tcgagctggc ggcccccgtg gttcgagacg acgactgcgg
    12901 ctgcgccgtg ctcgcacgca agccgcgcgt cctcggcggt gagaatcccc ttgacgacga
    12961 ccggaaggct gtagcccgcg agccactcga ggtcgtgcca gctgagaccg gtatcgaagt
    13021 gcccgccgaa gaggtcgccc gtgacggtga gatgctcggg aaccttgaag tcgatgcgga
    13081 ggtcgcgctc tcgccgcccg aggtagggcg tgtcgactgt gagcacgagc gcggagaagg
    13141 cgctctcgac tgcttcggcg atgaggactt ccgtctctga ccggtcggcg aagacgtaga
    13201 cctggaacca acgcggcgcg cctggagcgg ctgccgccac ctcggcgggc cgggcagtcg
    13261 ccgccgtcga gagacacatg atcgtgcccg ccgccgcagc cgcacgcgcg gttgcgagct
    13321 cgccgtcggg atgcgccatg cgctgcatcg caagcggcgc gatcaggatc ggtagagcaa
    13381 cgtcggtccc gaggatcgac gtcgcagtcg agatcgagct cacgtcgacg agcacgcgcg
    13441 ggcggagctt gcgccgctcg aacgccgcca ggttgtcgcg aagcgtgacc tcgtcccccg
    13501 ccccgccggc gaagtaagcg tgcgcgttca ggtcgagctt gcgttcggcg agctgctcgt
    13561 agtcccagac gttgaccggg ccggacacga gtcgagcgta accctgacag tccggacatg
    13621 tctcggggtc tgaccctaag acagtggctc gaaaggtcag ctgttgaaat cgggagtgtt
    13681 cgtgggggaa acagggtctc ccccacgcca aagcggagag ggcgggattt gaacccgcga
    13741 cgcacctttc agcacgtacg cgatttccag tcgcgctcct taggccactc ggacacctct
    13801 ccgtgctgcg aagcggagaa ggagggattc gaaccctcga tggaggcatt cacccccata
    13861 acgccttagc agggcgccgc cttcagccac tcggccactt ctccgcgggt ccgcgcagga
    13921 tagccga
//
LOCUS       DASVEU010000041        11209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1194073,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000041 DASVEU010000000
VERSION     DASVEU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194073"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..747)
                     /locus_tag="VF225_01505"
     CDS             complement(199..747)
                     /locus_tag="VF225_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7143958.1"
                     /translation="MGAAHVDETFWRQRAEELQHALDTRIVIEQAKGVLSERLGLDIE
                     GAFSVLRGAARFERVKAHALATAVVEDRDTPQTIVRFLARHPHFFHVSTRDERVARTQ
                     EFFKRVNDTMRKELGDGDGNFLCECGNIACTEKLALSSEDLRLLHSINGYLVVLPGHE
                     IPEIESLVKVNGRYAIVHNKMR"
     gene            complement(873..1688)
                     /locus_tag="VF225_01510"
     CDS             complement(873..1688)
                     /locus_tag="VF225_01510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7143959.1"
                     /translation="MTGGEEQTQTWHHGIVASWWAEFNVGGPEIEYFQKSIEDDGQPA
                     LDVACGTGRLLLPYLRAGLDVDGCDISSDMLALCRERAEAEGLSPNLFAQAMHELELP
                     RRYRTIVVCGGFGLGGNREQSFASLERIYEHLEPGGLLLLDNEAPYADAGLWRHWLKE
                     ERQALPKPWRELGEGDRRQGSDGTEYELRSRIVELDPLAQSVTLEMRADMWRNGEHVA
                     EEHVLRMSLYFTHELAAMLERAGFTDVSLTGDYRDEEPNSDTEFVVFRARKPL"
     gene            complement(1802..3208)
                     /locus_tag="VF225_01515"
     CDS             complement(1802..3208)
                     /locus_tag="VF225_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX7143960.1"
                     /translation="MATRRELPADIAAAFDRVPEAGSRFAALPPERQAAWLDWIDGAR
                     GRRARAGRIDELIRRSLPSAAVAEEEVAEPAGPPPERYWWLWLVLLLLLVVGGLLAWY
                     FLSRGSDKATVPNVIGLREDAAAVRIHDRGLDVLPRTDASNRPPGIVFAEKPGPGTQL
                     DKGQAVTIFVSSGRLGVPDVTGLPLPDAQQKLTAKGFKVEVRRVASSRPVGIVIGQQP
                     VAGVTAVSGTTVTLNVSSGVKPVVVPRVVGQTQGAAVDALTKLGLKPVLKNVPSDKPV
                     GTVVGQNPPTGKEVDKGAKVVVNVSTGSGPSTTTVATTTTTATTTTGATTTTASGPVR
                     VPRVVALAQTPALRRLNVLGLRPTVVYVRSTQPANRIVAQLPAPGTTLRRGSRVRINV
                     STGPNPEPATAVPNVAGQDQTTAAETLSAAGFEVAVLNRPTSDQSMDGLVVEQQPRAA
                     SRIPAGSQVTIFIGRFSG"
     gene            complement(3238..4680)
                     /locus_tag="VF225_01520"
     CDS             complement(3238..4680)
                     /locus_tag="VF225_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX7143961.1"
                     /translation="MDPLLAFDKLVGRSVERLLRSHYARRLRRIGWEHALEAPTSDLW
                     ASGEPPPRTGCKVEVLIDGAEALPRFEMELRKARSHVHLAGWFMTPDFGLVREPVRVE
                     LRTLLAELAERIPVRVLVWAGSPLPLFRPDRKDVVRTLHTLTSGTKIEAVADSKERPM
                     HCHHEKIAVIDDDVALVGGIDLTTESGDRFDTNAHPARGELGWHDAACLLRGPVVADV
                     AAHFAQRWHELTGDTLPEPSSSPPAGDVEAQAVRTVPEHVYESVRDGDFRILESYVRA
                     LRSAQRLVYLENQFLWSGEVVQILLDKIVQPPSDDFRLLIVLPANPNDGSDDTRGQLA
                     DLIEADNGAGRVLACTLRALGERGPCPIYVHAKIGIVDDRWLTLGSANLNEHSLFNDT
                     EMNVVTCNAELARATRVRLWAEHLERTQEEVSADAVRVVDELWKPIAEEQYERRQAGL
                     PPTHRLCLLPGVSRRSRRLLGPLQGLLVDG"
     gene            4703..5332
                     /locus_tag="VF225_01525"
     CDS             4703..5332
                     /locus_tag="VF225_01525"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7143962.1"
                     /translation="MAVSPTPLICLDPGHGTIPAIGRQTEPIGPGSSQLKIKDGGGTA
                     GEAPVALAIALRTRALLKRDGYRVAMTRTGRTYAGGNIERARFCNVRHASLMIRIHAD
                     GATDPSLNGIKTLVPALHAGWTDDIYAASVRAARKVQAAVARQTGARNLGLVQRSDLT
                     GFNWANVPAILVECGFMTNPTERRLLQSPSYQLKVARGLVAGAEAFAPR"
     gene            complement(5341..5769)
                     /locus_tag="VF225_01530"
     CDS             complement(5341..5769)
                     /locus_tag="VF225_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7143963.1"
                     /translation="MIWASHALETLEGAGFRSGGARRAVVELLGRQNCCMSAQEIFDS
                     LRRARRPVGIASVYRALETLADLRLVKRVDAGDGIARFEPARPDGDHHHHLVCRDCGK
                     VEAFSDARLERAIDKVAGGLGYSVEEHEVVLTGACADCAA"
     gene            5883..6653
                     /locus_tag="VF225_01535"
     CDS             5883..6653
                     /locus_tag="VF225_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7143964.1"
                     /translation="MVAAFYPLAYAAERIGGPAFDVQNLTPPGAEPHDLELTPREVAR
                     IQEASVVLYLSHGFQPAVSKAVEQARGKKVDILAGLPLHAADGAEAGLTADPHVWLDP
                     ILFERVANKIGTALGRPSTAVVADLSKLDGAYRQGLSDCKRHEIVTSHAAFGYLAARY
                     GLEQVSITGLAPETEPTPKQLAHVIQVVRRTHATTVFFETLVSPRLADTVAREVGART
                     AVLDPIEGLTPTEQKRGDNYLTLMRRNLAALRKALGCR"
     gene            6644..7336
                     /locus_tag="VF225_01540"
     CDS             6644..7336
                     /locus_tag="VF225_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7143965.1"
                     /translation="MPLALRLQRVSFAYGHAPPVLRDVDLEVERGEFVAIAGPNGGGK
                     TTLVRLVVGLEEPTSGRVELLVRKVGYLPQRAQAGIDAPVTVRELVTTGRASRTRLVG
                     PLSGADREAVRQAIDRVALTPQADRRLSTLSGGQQQRAFIAKALAAEPELLVLDEPTT
                     GVDVEAQDAIAALLQRLRNELGMTILYVSHEFGAVERFVERIVLVRGSIVFDGPPSGL
                     PDVWHDPSHAHA"
     gene            7329..8123
                     /locus_tag="VF225_01545"
     CDS             7329..8123
                     /locus_tag="VF225_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7143966.1"
                     /translation="MLELEFMRLAFGAGIVVGLLAPAVGFFLVQRRLSLVGDGIGHVA
                     FAGVALGYLLNLPLVLTALVVSVTGGLSIEWIRARRRTAGDQALALIFYTGIAGGVVL
                     VSAAGALNVNLFQFLFGSILTVTRGDLAVIAALGACALVTIALLYRPLVAVVVDEEGA
                     RVAGLPVAALNALTVALAAVTIALSMRIVGILLIAALMVLPVIAASRIAWSMRSTILL
                     GMGIGLGSVLAGITIAYYADLPPGGTTVLVAAATVLAAEFWSVVRG"
     gene            complement(8120..8392)
                     /locus_tag="VF225_01550"
     CDS             complement(8120..8392)
                     /locus_tag="VF225_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143967.1"
                     /translation="MKHPSAWLLGGFALFGFLRRRREAPLGPDPRADDLRRKLAESRS
                     IVEERDEFEAAEVTVDLAEPAPVDPDSRRRAVHDAGRATVERMRDL"
     gene            8505..8891
                     /gene="rpsF"
                     /locus_tag="VF225_01555"
     CDS             8505..8891
                     /gene="rpsF"
                     /locus_tag="VF225_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7143968.1"
                     /translation="MVAYEILLMLDPDLPEGRQEEIIKRARELVEKANGTWVRHDVWG
                     RRRLAYEIAHKGEGSYHLLNFDADPETLAELSRVLRITDGVMRHLAVRRVEGTAGAPP
                     PPEPEPISRSVPEPAYAEANTSSEEE"
     gene            8895..9341
                     /gene="ssb"
                     /locus_tag="VF225_01560"
     CDS             8895..9341
                     /gene="ssb"
                     /locus_tag="VF225_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7143969.1"
                     /translation="MANINRVVLVGNLTKDPELRHTPSGTAVCKLRIAVNTRQKDPQG
                     NWGDKPNYFDVTVWGNQGESCAQYLSKGRPVGVDGRLDWREWDAQDGTKRQAVEIIAD
                     TVQFLGSRDGGGGGGAGEGERQFVPAAATAGNEDFTSSSADDDIPF"
     gene            9422..9643
                     /gene="rpsR"
                     /locus_tag="VF225_01565"
     CDS             9422..9643
                     /gene="rpsR"
                     /locus_tag="VF225_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7143970.1"
                     /translation="MRRKSCFFCKDKVDEIDYKNVNQLRRYISERGKIRSRRITGACR
                     RHQRQVAVAVKRAREMALLPYVAEGREGR"
     gene            9651..10343
                     /gene="rplI"
                     /locus_tag="VF225_01570"
     CDS             9651..10343
                     /gene="rplI"
                     /locus_tag="VF225_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7143971.1"
                     /translation="MDVILRSDIDKVGLRGEVVNVTRGYARNFLFPRRLADEATPARV
                     AEVRKVEERRARNEAKTFEDAQSVAERLGQVELRFDVKAGPTGSLFGSVTPTDIADQL
                     WEKHKVRVDRRRIGGEPLKRIGRFQVPIELFQDVTVEVRTLVVPEGGELPPEEELEAL
                     AAVEEAEQTPPAEQEKHELTLDELDAVAAAQDAQLIAAGELQPSRGDVEGELQEDEFD
                     RAVDAGITEEQS"
     gene            10684..>11209
                     /locus_tag="VF225_01575"
     CDS             10684..>11209
                     /locus_tag="VF225_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7143972.1"
                     /translation="MTPVAQLAEAAPIAPPQNLEAEESVLGAMLLSPGAIGAVSEVLD
                     ASDFYRESHAKIYRAGLALYAKGEPVDAITLVDELEERGELEAVGGRVRMHELAALVP
                     ATANAGHYARIVREMATLRGLIAAGQQIAQLGWERPGETQILVDRAEQVVFELSQSRV
                     STEFSHIEELLKESF"
BASE COUNT         1837 a         3790 c         3785 g         1797 t
ORIGIN      
        1 ttcacctgga ccgcaactca ggcctgaccg ctttgtgcga cacgtaacaa atctgatagg
       61 ttttcgccgc gccttgacct cgcgcctggg cccgctttgc cgcatgagcc agccctctca
      121 cccgggatag acgcagacag atggcacgag gcttgccggt cgcggcgttt gcggtcaccc
      181 gcgaccggtg gcctccgttc agcgcatctt gttgtgcacg atcgcgtagc ggccgttcac
      241 tttgacgagc gactcgatct cggggatttc gtgaccgggc aggacgacga ggtaaccatt
      301 gatcgagtgc aggaggcgga ggtcttcaga gctgagggcg agcttctcgg tgcacgcgat
      361 gttgccgcac tcgcacagga agttgccgtc gccgtcgccg agctccttcc tcatcgtgtc
      421 gttcacacgc ttgaaaaact cctgtgtccg cgccacgcgt tcgtcgcgag tagagacgtg
      481 gaagaagtgc ggatggcgcg cgaggaaccg gacgatcgtc tggggtgtgt cgcggtcttc
      541 cacgacggcg gtcgcgaggg catgcgcctt caccctttcg aagcgggcgg cgccacggag
      601 cacgctgaat gctccctcga tgtccaggcc gaggcgctcc gacaagacgc ccttcgcttg
      661 ttcgatcacg atccgcgtat cgagcgcgtg ctgtagttcc tccgctcgct gacgccagaa
      721 cgtctcgtcg acatgcgctg cacccatatt ggcctctcgt cattcgtccg caggacgcgg
      781 aacggcgaga gatggacgga agcgggcctg ggtctagaga cgcaggccgg gcaagactcg
      841 acgcgtcgag cagccaaggt agcacctctt ggctacagcg gcttccttgc cctgaagacg
      901 acgaattcgg tgtccgaatt cggttcttcg tccctatagt cgcccgtcag gctcacgtcg
      961 gtgaagcctg cgcgttcgag catcgcggca agctcgtgcg tgaagtacag ggacatcctc
     1021 aagacgtgct cctccgccac gtgctcgccg ttccgccaca tgtcggcgcg catctcgagc
     1081 gtcacagatt gggccagtgg gtcgagctcg acgatccggc tccgcagctc gtactccgtc
     1141 ccgtcggaac cttgccgccg gtccccctcg cccaactctc gccagggctt cggtaatgcc
     1201 tgccgctcct ctttgagcca atgacgccac agtcccgcgt cggcataggg cgcctcgttg
     1261 tcgagcagca ggagacctcc gggctcgaga tgctcgtaga tgcgctcgag gctcgcgaaa
     1321 ctctgctcgc gattgccgcc caaaccgaaa ccgccgcaga ccacgatcgt cctgtacctg
     1381 cgtggcaact cgagctcgtg cattgcctgc gcaaacagat tcggtgacaa cccttcggct
     1441 tccgcccttt cgcggcagag cgcgagcatg tccgaggaga tgtcacagcc gtcgacgtcc
     1501 agcccggcgc gaaggtaggg gagaagcagg cgtccggtcc cgcacgcgac gtcgagcgca
     1561 ggctgaccgt cgtcctcgat ggatttctgg aagtactcga tctccgggcc gcccacgttg
     1621 aactcagccc accagctcgc gacgatcccg tgatgccagg tctgcgtctg ctcttccccg
     1681 cccgtcatct cgcgacgagg gtaatgcaag tttttccagc agcgcgggtc tgcctcgaag
     1741 gagcccggcg cgcttcgact gtggttgccg gtcagccgcc ggctgaagct gcgccgggct
     1801 cctaacccga aaagcggccg atgaagatcg tgacctgcga ccccgcgggg atcctggacg
     1861 ccgctctggg ctgctgctcg accacgaggc cgtccatgct ctggtcgctc gtcggccggt
     1921 tcagcaccgc aacctcgaaa ccggcggcgc tgagcgtctc cgccgccgtg gtctggtcct
     1981 gtccggccac gttggggaca gcggtcgccg gttccgggtt cgggcccgtc gagacgttga
     2041 tgcgcacgcg cgagccgcgc cgcagtgtcg tgcccggtgc gggcaactgg gcgacgattc
     2101 tgttcgcggg ctgggtcgaa cggacataga ccacggtcgg ccgaaggccc aagacgttca
     2161 gccggcgaag cgcgggcgtc tgcgcaagcg cgaccacgcg tggtacgcga acgggcccgg
     2221 aggcggtagt agtcgtagcg cccgtggtcg tagtggcggt cgtcgtcgtg gtcgcaaccg
     2281 tcgtcgtcga cggaccgctg ccggtcgaca cgttgacgac caccttcgcg cccttgtcga
     2341 cttccttccc tgtcggcggg ttctggccga ccacggttcc gacgggcttg tcggacggga
     2401 cgttcttcag aactggcttc aggccgagct tcgtcagcgc gtccactgcg gcgccctggg
     2461 tctgcccgac cacgcgcggg acgacgaccg gcttgacccc gctcgagacg ttcagcgtca
     2521 ccgttgtccc gctgaccgcg gtaacgcccg caaccggctg ctggccgatg acgatcccca
     2581 cgggacggga ggacgcgacg cgcctgacct cgaccttgaa ccccttggcg gtcagcttct
     2641 gctgcgcatc cggcaggggc agacccgtca cgtccgggac gccgaggcga ccgctcgaga
     2701 cgaagatcgt gaccgcctgg cccttgtcga gctgcgtgcc cgggccgggc ttctccgcga
     2761 acacgatgcc aggcggccgg ttgctcgcat ccgtgcgcgg gagcacgtcg agaccgcggt
     2821 cgtgaatccg aaccgcggca gcgtcttcgc ggaggccgat cacgttcgga accgtcgcct
     2881 tgtcactgcc gcggctgagg aagtaccagg ccagcaggcc gcctaccacg agcagcagca
     2941 ggagcacgag ccacagccac cagtagcgct cgggcggtgg gccggcgggc tctgcgacct
     3001 cttcctcggc cacggctgcc gacggcagcg accgccggat cagctcgtcg atccgtcccg
     3061 cgcgagcccg gcggccgcgc gctccatcga tccaatcgag ccaggcggcc tggcgttcgg
     3121 gcggtaacgc agcgaaacgg gagcccgcct cgggaacgcg atcgaacgcg gcggcgatgt
     3181 ccgctggaag ctctcggcgg gtggccatcg aagccgtgtt gccctcggcg agactaatta
     3241 accgtcgaca agcaggccct gcaacggacc gagcaatcgg cgcgagcgcc gagacacacc
     3301 agggagcagg cacagccggt gcgtcggtgg gagacccgcc tggcgacgtt cgtactgctc
     3361 ttccgcgatt ggcttccaga gctcgtccac gacgcgaacc gcgtcggcgg atacctcttc
     3421 ttgtgtgcgc tcgagatgct ccgcccacaa ccgcaccctc gtcgcccgtg caagctcggc
     3481 gttgcaggtg accacgttca tctcggtgtc gttgaacagc gagtgctcgt tgaggttggc
     3541 agagccgagc gtcagccaac gatcgtcgac gattccgatt ttcgcgtgga cgtagatcgg
     3601 acaagggccg cgctcaccga gcgcacgcag cgtgcaggcg aggacacgcc ctgcgccatt
     3661 gtccgcttcg atcaggtccg ccaactggcc acgcgtgtcg tcgctcccgt cgttcggatt
     3721 cgcgggcagc acgatcagca gccggaagtc gtcggacggc ggctgcacga ttttgtccag
     3781 aaggatctgc acgacctcgc ccgaccagag aaactggttc tctagataga cgaggcgctg
     3841 cgccgagcga agcgcgcgca cgtacgactc gaggatccgg aaatcgccgt ctcggacgga
     3901 ctcgtagacg tgctcgggca cggtccggac tgcctgcgcc tcgacgtccc ccgcaggcgg
     3961 gcttgagctc ggttcgggaa gcgtgtcgcc ggtgagctcg tgccaccgct gcgcgaaatg
     4021 ggcggcgacg tcagccacga cgggacctcg aagcaggcag gcggcgtcgt gccagccgag
     4081 ctcgccgcgt gcggggtggg cattcgtgtc gaaccggtcg cccgactccg tcgtcaggtc
     4141 gatgccgccg acgagggcga cgtcgtcgtc gatcacggcg atcttctcgt ggtggcaatg
     4201 catgggtcgt tccttcgagt ccgcgaccgc ctcgatcttc gtgccggacg tgagcgtgtg
     4261 gagagtgcgc acgacgtcct tgcgatcggg gcggaagaga ggcagcggcg agccggccca
     4321 aacaaggaca cgcaccggaa tccgctcggc cagctctgcg aggagcgtcc gcaactccac
     4381 tcggacgggc tcgcgcacga gtccgaagtc gggtgtcatg aaccagcctg cgagatggac
     4441 gtgcgaacgc gccttgcgca gctccatctc gaaccgaggt agtgcttccg cgccgtcgat
     4501 caggacctcc accttgcacc cggtccgagg cggcggctca ccggaggccc agagatccga
     4561 agtcggcgcc tcgagggcat gttcccagcc gattcgtcgc agccgccgcg cgtagtgcga
     4621 gcggaggagc cgctcgaccg accggccgac gagcttgtcg aatgcaagca gcgggtccac
     4681 cacaggcaga ctcttgcccg tgatggccgt ctccccaacc ccgttgatct gcctcgatcc
     4741 cggccacggc acgattccgg cgatcggtcg gcagaccgag cccatcggcc cgggctcctc
     4801 gcagctgaag atcaaggacg gcggcggcac tgccggagag gcgccggtcg ccctcgcgat
     4861 cgcgcttcgg acacgcgctc tgctgaagcg agacggctat cgcgtcgcga tgactcgcac
     4921 ggggcgcaca tacgcgggag gaaatatcga gcgcgcgcgc ttctgcaacg tgcgacacgc
     4981 gtccctgatg atcaggattc acgccgacgg cgcgaccgac ccgtcgctca atgggatcaa
     5041 aacgctcgtg cctgcgctgc acgccgggtg gacggacgac atctacgccg cgagcgtgcg
     5101 cgctgcccgc aaagtgcagg cggcggtcgc ccggcagacc ggcgcgcgca acctcggcct
     5161 cgtgcagcgc tcggacctga ctggcttcaa ctgggcgaac gtcccggcaa tcctggtcga
     5221 gtgcggcttc atgacgaacc cgaccgaaag gcggctcctg cagtcgccgt cgtatcagct
     5281 gaaggtcgcc cgcggtctcg tcgcaggcgc ggaagccttc gctccgcgct aggtatagcc
     5341 ctaggcggcg cagtcggcgc aggcgccggt gagcacgact tcgtgctctt cgaccgagta
     5401 gcccaggccg ccggcgacct tgtcgatcgc gcgctcgagc cttgcgtcgc tgaacgcctc
     5461 aaccttcccg cagtcgcggc agacgaggtg atggtggtgg tctccgtcgg gccgcgccgg
     5521 ctcgaagcgg gcaatcccgt cgcctgcatc gactcgcttc accagcctga gatctgcgag
     5581 cgtctcgagg gcgcggtaga cggaggcgat cccgaccggc cgcctggcgc ggcgaaggct
     5641 gtcgaagatc tcctgcgcgc tcatgcagca gttctggcgg cccaggagct ccacgacggc
     5701 acgtcgggcg cccccactcc gaaagccggc gccttcgagt gtctcgagtg catgactcgc
     5761 ccagatcacg ttcgcagtgt atcgtcccag acgtgatttc gagaatagtt ctcattcttg
     5821 tcctgtccgg ggtcgtcgca ggctgcgggt caggcggcgg cggctccgga ggagggaagt
     5881 cagtggtggc ggcgttctat cccctcgcct acgccgccga gcgaatcgga gggccggcat
     5941 tcgacgtcca aaatctgacg ccgcccggcg ccgagcctca cgacctcgaa ctgacgccgc
     6001 gcgaagtcgc acggatccag gaagcgagcg tcgtcctcta cctgagccac ggcttccagc
     6061 ctgcagtgag caaagcggtc gagcaggcgc gcggtaagaa ggtggacatc ctcgccggcc
     6121 tgccgctgca cgccgccgat ggcgcggagg ccgggctcac cgcggacccg cacgtctggc
     6181 tcgatccgat cctcttcgag cgcgtggcga acaagatcgg tacggcgctg ggtcgtccct
     6241 cgaccgcggt ggtcgccgac ctgagcaagc tcgacggcgc gtaccggcag gggttaagcg
     6301 actgcaagcg acacgagatc gtcacgagcc acgccgcgtt cggctacctc gcggcccgct
     6361 acgggctcga gcaggtctcc atcaccgggc tcgctccgga gacagagccg acgccgaagc
     6421 agctcgcaca cgtgatccag gtcgtccgcc ggacgcacgc gaccacggtg ttcttcgaga
     6481 cgctcgtctc gccgcggctg gcagacacgg tggcgcgcga ggtcggagcg cgcaccgcag
     6541 tgctcgatcc gatcgaaggt ctgacgccga ccgagcagaa gcgcggcgac aactacctga
     6601 cgctgatgcg ccggaatctt gcggcactgc ggaaggcact gggttgccgc tagcgctgcg
     6661 actgcagcga gtctcattcg cgtacgggca cgcacctccc gtcctgcgcg acgtcgatct
     6721 cgaggtggag cgtggcgagt tcgtggcgat cgcggggccg aacggaggcg gcaagaccac
     6781 gctcgtccgg ctcgtggtcg gactcgagga gccgacgtcg ggacgcgtcg agttgctcgt
     6841 gcggaaggtc ggttacctcc cgcagcgggc tcaggccggg atcgatgcac cggtcacggt
     6901 ccgcgagctc gtcaccaccg ggcgcgcgtc acggacacgg ctggtcggac cgctctccgg
     6961 cgccgaccgc gaagccgtgc gccaggcgat cgaccgcgtc gccctcacgc cacaggccga
     7021 tcgccggctg tcgacgctct ccggcggcca gcagcagcgc gccttcattg cgaaagccct
     7081 cgccgctgag ccggaactgc tcgtgctcga cgaaccgacg accggggtcg acgtcgaggc
     7141 gcaggacgcg atcgcggcac tcctgcagag gctccgtaac gagctcggga tgacgatcct
     7201 ctacgtctcg cacgagttcg gcgccgtcga gcgcttcgtc gagcggatcg tgctcgtccg
     7261 cggcagcatc gtcttcgacg gccctccttc aggcctgccc gatgtctggc acgacccctc
     7321 gcacgcccat gcttgagctc gagttcatgc ggctcgcgtt cggcgccggg atcgtcgtcg
     7381 ggctgcttgc gcccgccgtc ggcttctttc tcgtgcagcg acgtctcagc ctggtcggcg
     7441 acgggatcgg ccacgtcgcg tttgcgggtg tcgcgctcgg ctatctgctc aacctgccgc
     7501 tcgttctgac ggccctcgtc gtctcggtca ccggaggcct gtcgatcgaa tggatccgag
     7561 ctcgccggcg aaccgcaggc gaccaggcgc tcgcgttgat cttctacacc gggatcgcgg
     7621 gcggcgtcgt gctcgtgtcg gccgccggcg cgctcaacgt gaacctcttc cagttcctgt
     7681 tcggctcgat cttgacggtc acgcgaggag acctggccgt gatcgccgcg ctcggcgcct
     7741 gcgcactggt cacgattgcg ctgctctacc ggccgctcgt cgcggtcgtg gtggatgagg
     7801 aaggcgcgcg ggtcgcaggg ctaccggtcg ctgcgctcaa cgcgctcacc gtcgcgctcg
     7861 ccgccgtgac gatcgcgctg tcgatgcgca tcgtcgggat cctgctgatc gccgcgctga
     7921 tggtgctacc ggtgatcgcc gcgtcgcgaa tcgcctggag catgcgctcg acgatcctgc
     7981 tcggcatggg aatcgggctc ggctcggtgc tggccgggat cacgatcgcc tactacgcgg
     8041 acctcccacc cggcggaacg accgtgctcg tcgccgcggc gaccgtgctc gccgccgagt
     8101 tctggtccgt cgtacgcggc tagaggtcgc gcatgcgttc aaccgtcgcc cgtccggcgt
     8161 cgtgcactgc gcggcgtcgc gaatcgggat caacgggtgc aggctcggcg aggtcgacgg
     8221 tcacctcggc ggcctcgaac tcgtcgcgtt cctcgacgat cgagcgggat tccgcgagct
     8281 ttcggcgcag atcgtcagcg cgcggatcgg ggccgagagg ggcttcgcgc cgccggcgca
     8341 ggaagccgaa cagggcgaac ccgccgagca gccacgcgga cgggtgcttc atcgcggcga
     8401 tgctacactg gccgccttcc tgcctcgcca tcgcgctccc tttcccgagt ggcgccagcc
     8461 ggtgaggccg attcagacca aaggaaggga ggtctcggga actagtggtt gcgtacgaaa
     8521 ttttgctcat gctcgatccg gacctgccag aagggcggca ggaagagatc atcaaacgcg
     8581 cgcgtgagct cgtggagaag gccaatggga cgtgggtccg gcacgacgtc tggggacgac
     8641 gccggctggc atacgagatc gcccacaaag gcgagggctc gtaccacctt ctgaacttcg
     8701 acgccgaccc ggagacgctc gcggagctgt cacgcgtcct ccgcatcacc gacggcgtca
     8761 tgcggcacct cgccgtgcgc cgggtcgaag gcacggccgg ggcaccgccg ccgccggagc
     8821 ccgagccgat ctcgcggtct gtccccgagc ccgcctatgc tgaagcgaac accagttcgg
     8881 aagaggagta gaagatggcc aacatcaatc gcgtcgtgct cgtggggaac ctgacgaagg
     8941 acccggagct gcggcacacg ccgagcggca ccgccgtctg caagctgcgg atcgccgtga
     9001 acacccgcca gaaggatccg cagggcaact ggggcgacaa gcccaattac ttcgacgtca
     9061 ccgtctgggg caaccagggt gagagctgcg cgcagtacct ctccaagggc cgcccggtcg
     9121 gcgtcgacgg acgcctcgac tggcgcgagt gggatgcgca ggacggcacc aagcgccagg
     9181 cggtcgagat catcgccgac accgtgcagt tcctcggcag ccgcgatggc ggcggcggag
     9241 gcggcgcagg cgaaggcgag cgccagttcg tcccggcagc ggcaacggcc ggtaacgagg
     9301 acttcacctc gtcctcggca gacgacgaca tcccgttctg atgccgccgc gcggaggagg
     9361 agaaaagaga gacaaggacc ggcagcccgg ccgtaagcgg ccgggccagt cctctggtcc
     9421 gatcaggcgg aagtcgtgct tcttctgcaa ggacaaggtc gacgagatcg actacaagaa
     9481 cgtgaaccag ctgcgccggt acatctcgga gcggggcaag atccgctccc ggcgcatcac
     9541 cggcgcgtgt cggcgtcacc agcggcaggt ggccgttgcc gtcaagcgcg cgcgggagat
     9601 ggcgctcctg ccgtacgtcg cggaaggccg cgagggtcgt tagtgcacca atggacgtaa
     9661 tcctccgttc ggacatcgac aaggtcggtc tgcgcggcga ggtcgtgaac gtcacgcgcg
     9721 gctacgcgcg gaacttcctc ttcccgcgcc gcctcgccga cgaggcgacc ccggcgcgtg
     9781 tcgccgaggt gcgcaaggtc gaagagcggc gtgcgaggaa cgaggcgaag acgttcgagg
     9841 acgcacagtc tgtcgcggag cggctcggcc aggtcgagct tcgcttcgac gtgaaggccg
     9901 gccccaccgg ctcgctgttc ggatcggtca cgccgacgga catcgccgac cagctctggg
     9961 agaagcacaa ggtccgtgtc gaccggcgca ggatcggcgg cgagccgctc aagcggatcg
    10021 ggcgtttcca ggtgccgatc gagctgttcc aggacgtgac ggtcgaggtg cggacactcg
    10081 tcgtgcccga gggtggcgag ctgccgcccg aggaagagct cgaggcattg gcggctgtcg
    10141 aggaagccga gcagacaccg cctgcggagc aggagaagca cgagctgacc ctcgacgagc
    10201 tcgatgcggt cgctgccgcg caggatgctc agctgatcgc ggccggagaa ctgcagccgt
    10261 cacgcggcga tgtcgagggt gagctacagg aagacgagtt cgaccgcgcg gtcgacgccg
    10321 gcatcacaga agagcaaagc tagccgcctc aacagccgta cgacgccaga cgcaacggct
    10381 tgcgtcgcag cagcgatcaa gactccgact gcgccgatca tgtccacaat cctgcgcctg
    10441 ttttaaagcg gggccgaggc agttctgtgg aaagcctggg tacaaccttt cacaggcctt
    10501 tccaccgcct gtggacttgc cgaagaagtt cccgctccta gcggtcgaac catcaacagg
    10561 ctccgtccga accgtatggg aacatgtgtt cttttccgct gcaaacaggg cgaaaaggac
    10621 atgtcaggaa cccaccttcc cacagatgga aagacgcgca caagggtggt tcactccgac
    10681 cgagtgacac ctgtcgccca gctcgctgaa gccgctccga tcgcgccacc ccagaacctc
    10741 gaggcggagg agtccgtcct cggggcgatg ctgctctccc cgggcgcgat cggggcggtg
    10801 agcgaggtcc tcgacgcctc cgatttctac cgggagagtc acgcgaagat ctaccgcgcg
    10861 ggcctcgcgc tctatgcgaa gggcgagccg gtcgacgcga tcacgctcgt cgacgagctc
    10921 gaggaacgcg gtgagctcga ggccgtcggc ggtcgggttc gcatgcacga gctggcggca
    10981 ctcgtgccgg cgaccgccaa tgcggggcac tacgcgcgca tcgtccggga gatggcgacg
    11041 ttgcgcgggc tcatcgccgc gggacaacag atcgcccagc tcggctggga acgcccaggc
    11101 gagacgcaga tcctcgtcga ccgggccgag caggttgtct tcgagctttc gcagagccgc
    11161 gtttccaccg agttcagcca catcgaggag ctcctgaagg agagcttcg
//
LOCUS       DASVEU010000042        23185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1389237,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000042 DASVEU010000000
VERSION     DASVEU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 23185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23185
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389237"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VF225_01580"
     CDS             complement(<1..1015)
                     /locus_tag="VF225_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143973.1"
                     /translation="MPKRADLRSVLIVGSGPIRIGQGCEFDYAGAQACRVLRREGYRV
                     VLVNSNPATIMTDPEWADATYLEPLDADTIAEVIERERPDALLPTLGGQTALNLTVEL
                     AEAGVLSRFGVELIGADVDAIHRAEDRLEFRAAVTAAGVPVPESRVVSSADELDGISL
                     PAVVRPAFTLGGTGGGIVRMREELEALVRRGLAVSPIGQVLVERFLEGWQEHELEVMI
                     DIAGNCVVVCSIENLDPMGVHTGDSWTVAPQQTLPDGEYQRLREAAFACARAVGVATG
                     GANVQFAYEPASRELAVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDELPND
                     ITGK"
     gene            complement(982..2034)
                     /gene="carA"
                     /locus_tag="VF225_01585"
     CDS             complement(982..2034)
                     /gene="carA"
                     /locus_tag="VF225_01585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7143974.1"
                     /translation="MQTIRPAKLVLEDGTVFGGAAVGAPGVAAGEACFTTAMAGYEEA
                     ASDPSYMAQVLAFSYPLIGNYGVDSSRLESDGVCAEAIVMRRARPAWKQWLASQGVVA
                     LEDVDTRALVRKIRDGGVLRCALGEASVAELHARALAEPPIDGRPLGRSVGTAQPYRL
                     GTGAPITVLDLGTKRSIPTRLAACGLEVMVVPGDWDAEAILENVPRAVLVSNGPGDPA
                     VLAGPIETVRDLLGRVPVFGICLGHQILGLALGLETFKLPFGHRGANHPVRDLRTGRV
                     LVTVQNHGFAVADSGGDVEVTHVSLNDGTVEGLAGDGFATVQFHPEAAPGPLDALPFF
                     ELIADTCRSAPTFALS"
     gene            2066..3046
                     /gene="argC"
                     /locus_tag="VF225_01590"
     CDS             2066..3046
                     /gene="argC"
                     /locus_tag="VF225_01590"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7143975.1"
                     /translation="MFSAALVGCSGYAGQEALDRVLAHPELKVVALGSDSLAGKPPSS
                     LDLRLNGSLPTFVPNAEALGSGADVLFLCLDHDEAAALEPPPEAVVVDLSGGHRLHDA
                     DLYGDWYGFAHPFPSALAEWSYALPELYPPAGRLIANPGCYSTAALLALAPLADGIEP
                     EGIVVDAKSGVSGAGRALKASSHAGFVLENLSPYRIGAHQHAPELEQALGFPVCFVPH
                     LLPIRRGLLATCYMRATDGASRERLEDAYADSDVVRVLPEGVAPELNRVQCTDTAEVA
                     LFEDRSTGTTIVVCALDNLGKGAAGQAVQNANLALGLDETAGLRLTGVPV"
     gene            3043..4206
                     /gene="argJ"
                     /locus_tag="VF225_01595"
     CDS             3043..4206
                     /gene="argJ"
                     /locus_tag="VF225_01595"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7143976.1"
                     /translation="MSVTAAKAFVASGVHSGLRRSAPDLAIVRSLAPATGAAMFTANR
                     MQAAPVVISKEHLELAEPQAVVINSGSANAATGLRGELDARATAAEAGRLLGLAVEEI
                     VVLSTGVIGKRLALDKILPALRTATDSLSERGGGNAAEAILTTDTHAKQAVANGPGFA
                     VGGMAKGSGMIHPNLATMLAVVTTDYPLEPGETSAFLRTAVDESFNAISVDGECSTND
                     TVVLLANGASGATRSPASDAAFDECLRAVCADLAGQIVTDGEGATFTAAISVSGAASD
                     LEAKAIARRIATSPLVKTALFGRDANWGRVLAAAGSAPFNGGYAQLDPDCVTLDLAGV
                     RVVDSGAPQNVEPSLDGAGCTIVLDLALGEGSASYLTSDLSYDYVRINAEYRS"
     gene            4203..4871
                     /gene="argB"
                     /locus_tag="VF225_01600"
     CDS             4203..4871
                     /gene="argB"
                     /locus_tag="VF225_01600"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7143977.1"
                     /translation="MSLVVLKCGGAVACESADTILQLADAGHDVCVVHGAGPQISEEM
                     ERFGIPVEFVGGRRVTTEAGLAVVRSSFAAVNRALCTALGTRAVGLFGDEIGLHAVEI
                     PALGRVGDPLPSRPQVVVDAFVSGHIPVVAPLGVGPLNVNADDAAAALAVGLEADRLL
                     FVSDVAGVFRDGSVLTSIGAGEADRLLGDGTFAGGIVPKLRAAVIAARNGVRTEIGET
                     AVVA"
     gene            4868..6004
                     /locus_tag="VF225_01605"
     CDS             4868..6004
                     /locus_tag="VF225_01605"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HEX7143978.1"
                     /translation="MTVVAHSPVLPVYARQDLTFVRGEGTTLHDPDGRAYLDFLAGIA
                     VVGLGHCHPAPLAAAHAQLDRLWHVSNLYWTEPMVELAQRLSARAGGARAFFCNSGAE
                     AVEASLKYARKVTGKPGVVALSGSFHGRTMGALSVTGQPGKREAFEPLLPHVQFAGPN
                     DIASLEAAISAETGCILLEPVQGEGGVHVLEAAFVAAARRIADETGAVLLFDEVQTGV
                     GRTGAFFAYEHVSVRPDALMLAKGLANGLPIGCLLVFDEAPEGFAAGDHASTFGGNPV
                     ACAAASAVCDELTDELLATVKANGLLLADGLAALPGVREVRGAGLLLGAELDRPAADV
                     VAACTERGLLVGTAGANVVRLTPPLIVSTAEIEDALGVLEEVLV"
     gene            6001..6462
                     /locus_tag="VF225_01610"
     CDS             6001..6462
                     /locus_tag="VF225_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143979.1"
                     /translation="MRKFERQGVILRLVSEQELSTQTELAAALHEAGFDAVQTTVSRD
                     VAELGLVKVRNEEGRLVYAPPGAADLSRLGELASALRRWALSIVPTGTIVVIQTPPGH
                     ANALARAIDGAGLADVAGTVAGDDTIFVAAREGSGTALADQLQHHLEGESQ"
     gene            6459..7661
                     /locus_tag="VF225_01615"
     CDS             6459..7661
                     /locus_tag="VF225_01615"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7143980.1"
                     /translation="MNGIAVLAYSGGLDTSCAIAWLKEDYGFDEVVAVLVDVGQEASF
                     HPAIERGKLAGADDVLLLDRKEAFADEQVAKALIANALYEGKYPLVSALSRPVIAEAV
                     AEVAVELGADAVVHGCTGKGNDQLRFELAFKAKYPGVKVIAPLRDRVWTRDAEIAYAE
                     ERGIPVEAKAESPYSVDDNLFGRAIEAGILEDPWTAPPEDAFLLTGDLSVAPAPTDVT
                     VSLEQGLPVSIDGEELPLFALVAVLNERAGAYGIGRIDMIENRAVGIKSRELYEAPAA
                     LTLIAAHRALEELVLTKGELEAKRELEPKWAKLVYDGGWFAPVRGAYDAFFAATQELV
                     TGDVRLSLQPGAAVVTGRRSDHALYSESLASYGIGETFPHHEAAGFIGITALETQLVA
                     ARTQVQVA"
     gene            7658..8884
                     /gene="argH"
                     /locus_tag="VF225_01620"
     CDS             7658..8884
                     /gene="argH"
                     /locus_tag="VF225_01620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7143981.1"
                     /translation="MTLWSGRVESDVAQAVWDFLRADDAELLPYDCLATRVHARRLHE
                     GGLLSDAELADVETALNWIAVEGAVEPEDEDVHSTIERLLGTIGRKIHAGRSRNDQVA
                     AALRLYVADASLEAVTAIRGFVVSVLARAEEEAETPMPGYTHLQRAQPITVGHHLLAW
                     VEMLDRDRDRFRLAAEAARPWPLGAGALAGSTLELPPPPGAQLRNSLDAVGDRDFALD
                     YLYACAMLATHLSRVGEEIVLWCTTEFGFVRLPEEAATGSSMMPQKLNPDVAELARGK
                     AGAAIGRLTGLLATVKGLPLAYNRDLQEDKEPVFKARRDVGGTLAALEVLVTGIEFDH
                     ERLAAACSDPLVSATDAAEALVAEGVPFRDAHEHVAGQVREGTFQAPELPRPRGAPGP
                     ADVYAAVAEARARFDA"
     gene            complement(8924..9235)
                     /locus_tag="VF225_01625"
     CDS             complement(8924..9235)
                     /locus_tag="VF225_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX7143982.1"
                     /translation="MTQRLGFVLRVKPDRVDEYVLAHREVWPEMLEALKSAGIRNYTI
                     FRAGNEMFGYFETDDLARTETYLAAQEVNALWQDAMADLLEERVQDQGPSPLEEIFRL
                     A"
     gene            complement(9237..9854)
                     /locus_tag="VF225_01630"
     CDS             complement(9237..9854)
                     /locus_tag="VF225_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX7143983.1"
                     /translation="MNEARDEILARTRAAISEGAPPDPVARAYRTTGVLDDAGRVALL
                     SERLLDYRARVERTTATALRDLLGELARDGRIAVPRGLPEKWRPPNSVEDDGLSTHEL
                     DALDGVITGCTVAIAETGTLILSGGEMEGRRALTLVPDVHICVVRTDQIVETVPEAFA
                     ALRGRERRPLTLVSGPSATSDIELERVEGVHGPRTLIVVILKEAD"
     gene            complement(9838..11202)
                     /locus_tag="VF225_01635"
     CDS             complement(9838..11202)
                     /locus_tag="VF225_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX7143984.1"
                     /translation="MTSFPEAARIALGDSQLRHNLRNATTTIRDKRARVVAEVPDWEE
                     LREAGRAIKEDVMRRLDEYLLQFEESVQAAGGQVHWARDAAEANAILLDVARAHDATE
                     VVKVKSLTTDEIGLNEALATGGVRAIETDFAELILQLDGDWPSHILVPAIHRNRSEIR
                     DLFMRTIADGRELSDDPRELAETARLYLREKFLEAKVGVSGANFGVAETGTVVVVESE
                     GNGRMCTTLPPVLVTVLGIEKLVPTFADLEVFLQILPRSSTGERMNPYTSLWTGVTIG
                     DGPQEFHVVLLDNGRTRVLSDEVGRQALHCIRCSACLNVCPVYTRTGGHAYGSVYPGP
                     IGAILTPQLRGVANAKSLPYASTLCGACYEVCPVKIDIPSVLLHLRAQTEKDVAERIA
                     MRALARIFGNARLYEWAQKLARAGERPARRVPGPLAAWTQTRDLKPFAKETFREWWSR
                     ERGA"
     gene            complement(11199..11885)
                     /locus_tag="VF225_01640"
     CDS             complement(11199..11885)
                     /locus_tag="VF225_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7143985.1"
                     /translation="MNSRMRIGLFVTCLGDTLFPEAPRAAVTVLERLGHDVVFPREQT
                     CCGQLHQNSGYLDEAQALARRFARIFGDFDAVVSPSSSCVGAVRELSPEIAPRVFELS
                     EFLVGQLGVEDVAAYFPHRVTYHPTCHSLRVVQVGAAPLRLLRGVRGLELVELPEAAE
                     CCGFGGTFAVKNADTSTAMLEDKCRCVLATGAEVVTAVDASCLMQIGGGLSRQGTGIR
                     TMHLAEILAS"
     gene            complement(11895..13328)
                     /locus_tag="VF225_01645"
     CDS             complement(11895..13328)
                     /locus_tag="VF225_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEX7143986.1"
                     /translation="MKQSAAPRVGIFGIGLAAYWPQFEGLRERLEGYQRDIQARLEAM
                     GAEVVSAGLVDTAPAARDAGANLAAARVDLVMVYPATYATSSQVLPIVQAVGAPVVIL
                     NLQPTRTLDYEGMTTGEWLANCSACCVPEIAGALTRARIHYRTVTGTLLDGDPAWDVL
                     HEWLDAAHAVHSLRTARIGFLGHTYPGMLDMYSDFTQVQAQTGAHVEVLEIDDLVERV
                     ESAKAAEIERKGEEIRETFDLADAGVDPIAADITPEIFDWSARVAVGLDRLVEDFELD
                     GLTYYYRGIGGNAAERVTAGLIVGNSLLTARGVPASGEGDLKTNLGMLLLDRLGAGGS
                     YTEFYALDFDDAFVLMGHDGPGHLAIAEGRPVARALKLYHGKAGAGLSIEMKVRRGPV
                     TILGVTQTADGRLKLIAAEGESIAGPTFRIGNTNSRIRFAAAPTAFFDAWCAEGPTHH
                     VALGVGHQVARLRKVGDLLGLELAVVE"
     gene            complement(13325..14398)
                     /locus_tag="VF225_01650"
     CDS             complement(13325..14398)
                     /locus_tag="VF225_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143987.1"
                     /translation="MSDLGFQPHEEIPGTEAPRRTGIGVFLRWEVLLLVLLGGTIAYG
                     AAVSPYFLHSTNLFYISLNVGEVAIMALPLTLIVVTGEIDLSVASMLGLSGVFMAELF
                     THGWPIWPAMIMAVVLGAFLGAVNGFLVTRMGLPSLAVTIGTLTLYRGIAQGILPTDT
                     IGGFPAGLASIGVVPIPHTHIPYSIAFFLALAVVFGIVLHATPLGRAIFAIGAGHEAA
                     FFAGIRVKRIKFWLFVLSGTLSGFAGVLWTLRFASARYDAGVGMELLVVTIVLLGGIS
                     IFGGRGTIAGVIVAVAILGTLQTALTADLIPAQDQNIVVGALLIASVIIPNGGELYRR
                     ARARLRSAEARRHAAAAAQGEGQ"
     gene            complement(14391..15431)
                     /locus_tag="VF225_01655"
     CDS             complement(14391..15431)
                     /locus_tag="VF225_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7143988.1"
                     /translation="MTAVAEEQKVVQPYASSRAFAEVLFRFRELGIVLALVLVVGATT
                     IDNHLFLSATNVQQILSGASIIALLAIGETIVIITRNVDLSIGSVLGISAYASGELYV
                     HHPHVPLALVFLLALGIGVGCGIVNGLIVTVARVPSLVVTLGTLYIIRGIDGAWAGGN
                     QVNASMLPNTFNKIGYGTVAGVPYLGIIAIVAVAIATYAMRSFRTARDFYAIGSDPDA
                     ARVAGIPVGARVFLAFVLSGAIAGVAGAVWLSYYGSVDAIAGAGYEFQVIAAVVVGGV
                     AIFGGSGSVLGAALGALLLNTINSALVVVNVSSFWSQALAGGLLVGAIAFDRLIAVRV
                     APALRTRRRERV"
     gene            complement(15428..16918)
                     /locus_tag="VF225_01660"
     CDS             complement(15428..16918)
                     /locus_tag="VF225_01660"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7143989.1"
                     /translation="MLALEHAQKSFGAVHALEDGDIELFGGEVHGLVGENGAGKSTLV
                     KILAGVHRPEGGRLVLDGEEAIFDNAKQSQAAGIAIIFQEPTLFPDLSVAENIFVGMQ
                     PLKRLRRIDGRRMRREAAAVFEQLGVRLDPDRLARGLSIADQQLVEIAKALTSNARVI
                     VMDEPTAALAGTEVDRLFGIVESLRSRGNAVLFVSHRLDEIFEISQRVTVMRDGRHVL
                     TKPIDELTIQSVIRAMVGRDMDALFPKVPTEPGPVVLEVNRLTREGVFTDVSFNVRSG
                     EVVALAGLVGAGRTEVARAIFGIDRWDAGAIEISGRRLRPGSPTAAMRAGIGLVPEDR
                     RQQGLVMDFSIERNIALASLDNIQRAGLIPRGAERSFAKDWALRLQLKYGKLTNPVWT
                     LSGGNQQKAVLAKWLARKPTLLIVDEPTRGIDVGTKAEVHRLLSELAAQGVAVLMISS
                     ELPEVLGMADRIIVLFEGRVMREFTRAEATEDALMHAATGMVEEAA"
     gene            complement(16997..18013)
                     /locus_tag="VF225_01665"
     CDS             complement(16997..18013)
                     /locus_tag="VF225_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7143990.1"
                     /translation="MHKRRWTIIVAAVLVAGVAVTAALAANSRSTAGKTFYFIPKDTL
                     NPYEVIADRGGKIALTKLGHKQVVSSGTEDTAAAQQPAIQAAIQAHAAGIVIAGNDPQ
                     AVCPALNQAKAQGTKVIAFDSDVNCRQLFINQADTPTIGRSQIKLLGKLMKYKGEFAI
                     LSAASTATNQNAWIKYMKLELKKPVYKNMKLVKIYYGNDNPAQSRQATVSMLQAYPNL
                     KGIESPTTVGISSAAQYLSTSKYKGKVVLTGLGLPSQMKKYVHDKTVTAFQLWNPEDL
                     GYLAGYAIAAFADGKVTGKVGQTFTAGKLGKYTIINGPDKRPQVILGPPFTFTIKNVD
                     KFKF"
     gene            complement(18076..20187)
                     /gene="rhaD"
                     /locus_tag="VF225_01670"
     CDS             complement(18076..20187)
                     /gene="rhaD"
                     /locus_tag="VF225_01670"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX7143991.1"
                     /translation="MSVAKMLGGIPAPEDRWDDASSAGLSSLDALVYRSNLLGSDRAL
                     ANQGGGNTSSKGTAVDHAGREQRVLWMKGSGTDLASIAPSGFAVLRLDEIIPLRRREE
                     MDDTAMVGFLRRSALDPDQPRPSIETLLHAFIAAPQVDHTHPDAIIALTSSPNGRKLA
                     EETFGEEVVWLDYQRPGFDMSRRIAELLDANPSAHAVLLEKHGLVTWGESGEESYRRT
                     IEFVSRAARAIEKAGAGRFGLGGPKVAECGEQDAQFLLLASLPALRGALLTDADGVVL
                     EVDRSPEAVAFASSVRAPDVSQVGAPCPDHLINTKHKPLAIEFDPSSAGAAELTDAFR
                     DGVAAYVDWYREYYERNVSDESRPFPMDPAGPRVVLIPGVGIVTTGADATRTRTARDL
                     YHRAIAVEDAANAIGGFRSLTEAEAFAIEYWPLERYKLAQAPPPRELAGRIAVITGGA
                     SGIGRATARLLAHLGAHVVVADLNREGAEAVAEEIVGACGLRRAVAAHVDVTDENAVA
                     EMMEQTVLEYGGLDILVASAGLATSAPVTETTLEEWELNYAVLARGYFLCAREAFRVL
                     LKQDRAGSIVFVGSKNALVAGANAAAYSSAKAASLHLARCLAEEGGPHGIRVNTVNPD
                     AVIQGSGIWSSDWKAERASTYGVTEDDLQSFYSGRTKLGVSVYPEDVAEAIAFLAGPR
                     SAKSTGNVINVDGGVTAAYPR"
     gene            complement(20184..21674)
                     /locus_tag="VF225_01675"
     CDS             complement(20184..21674)
                     /locus_tag="VF225_01675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEX7143992.1"
                     /translation="MATFAAIDLGAQSGRVAVGRFDGERLSVSKVHRFPNVPVRTRNT
                     LNWDILRLYQDVTDGLRAAAREAGAVDSVAVDSWAVDFGLLDQNGRLLQNPVHYRDDR
                     RARAVEGVLAEVPARDLYERTGIQLIPINTVFELGAMAAEHDPALEVAETLLLIPDLM
                     HYWLCGARTSELTNATTTQCFDPRTGTWAVDVLERLDVPTRLLPEVVRPGTKLGRLSE
                     RVATDTGLGGALVVAVATHDTGSAIAAVPFRRPSSAFISAGTWSLVGLEVQQPVISDA
                     SFAANLTNEGGVGSTFRLLRNVTGLWLLHECRRAWALEGHDYSFDQLVALAKEAPALR
                     SLIEPNDPAFSDQGDMPARVRAFCAHTGQPEPFEPGAVVRCILESLALKHAQTIDVLA
                     SVTGTAPEEIHLVGGGSRNDLLCRWTAGAAGLPVLAGPEEATLLGNFLVQAMSLGELA
                     SIDEGREIVRASFAPTIYEPQDTAVWREARERFARAVALPTLEVGL"
     gene            complement(21674..22495)
                     /locus_tag="VF225_01680"
     CDS             complement(21674..22495)
                     /locus_tag="VF225_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7143993.1"
                     /translation="MQHVLHLLESRDYLPVAELSEAFAVSEVTVRSDLTTLAKQGLVA
                     RIRGGVRALQLGQSEVGFDHRLRLEVERKRAIARAAAAMVDEGEAVALDASTTAYYLA
                     LELRSKRELVVVTNGLLVATALADAPGITVLVTGGMLRLSAMSLVGDLGADVLRTTRI
                     NKGFLGARGLSISRGLMDLNPDEVSIKQEMADACEQVYGIFDGTKWHRSALLAFVRAE
                     DLAGIVTDSSAPAAEVGVWRAAGVDVLTVDPGPREPLPLRPRDLRRAVRNDEAGG"
     gene            22661..>23185
                     /locus_tag="VF225_01685"
     CDS             22661..>23185
                     /locus_tag="VF225_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7143994.1"
                     /translation="MNGDAMFSRLDALEIETPSWGYGNSGTRFHVYPWPGAARTVWER
                     IDDAALVHELAGCCPSVALHIPWDTVDDWSALGDYARGRGIRIGAINPNLFGDDSYRL
                     GSLANPDPAIRRRALEHCIECVEIARQLGSTIVSLWLADGTNYPGQDDLRGRHARLLA
                     GLEELYAVLSPNQQL"
BASE COUNT         3830 a         7896 c         7733 g         3726 t
ORIGIN      
        1 tcttgccggt gatgtcgttc ggcagctcgt cgagcgtgta gccgacggcg agcagggcag
       61 cgatcttcgc gatcgggaac cccgtggcct tcgaggcgag cgcggaggag cgcgagacgc
      121 gtgggttcat ctcgatgacg gcaagctctc ggctcgcggg ctcgtacgcg aactgcacgt
      181 tcgcgcctcc cgtcgcaacg ccgacggcgc gcgcgcatgc gaacgccgcc tcgcgcaggc
      241 gctggtattc gccgtcggga agcgtctgct ggggcgccac ggtccacgag tcgccagtgt
      301 ggacgcccat cggatcgaga ttctcgatcg agcagacgac gacgcagttg ccggcgatgt
      361 cgatcatcac ttcgagctcg tgctcctgcc agccttcgag gaagcgctcg accagcacct
      421 ggccgatcgg gctcaccgca aggccccggc ggacgagtgc ctcgagctct tcccgcatgc
      481 gcacgatgcc gccaccggtg ccgccgagcg tgaatgccgg ccgcacgacg gccggcaagc
      541 tgattccgtc gagctcatcg gccgacgaga cgacacgact ctcgggaacg ggcacgcccg
      601 cggccgttac agcggcgcgg aattcgagcc ggtcctcggc gcgatggatt gcgtcgacgt
      661 cggcgccgat caactcgact ccgaagcggg acagcacgcc cgcttcggcg agctcgacgg
      721 tcagattcaa cgcggtctgc ccaccgagag tcgggagcag cgcatcggga cgttcgcgtt
      781 cgatcacctc cgcgatcgtg tcggcatcga gcggctcgag atacgtcgcg tcggcccatt
      841 cggggtcggt catgatcgtc gccgggttgg agttgacgag cacgacccgg tagccctcgc
      901 gccggagcac acggcacgcc tgcgctccgg cgtagtcgaa ctcgcacccc tgcccgatcc
      961 ggatcgggcc ggagccgacg atcaggacag agcgaaggtc ggcgcgcttc ggcacgtgtc
     1021 cgcaatcagt tcgaagaagg gaagcgcgtc gagcgggccg ggagccgcct cggggtgaaa
     1081 ctgcacggtg gcgaacccgt cgccggcgag tccttcgacc gtcccgtcgt tgagcgagac
     1141 gtgcgtgacc tccacgtcgc cgccgctgtc tgcgaccgcg aagccgtggt tctgaacggt
     1201 cacgagcacc cggcccgtcc tcaggtcgcg gacggggtgg ttcgcaccgc ggtgtccgaa
     1261 cgggagcttg aacgtctcca gcccgagcgc gagcccgagg atctggtgac cgaggcagat
     1321 accgaagacc ggcacgcgcc cgagcaggtc gcggaccgtt tcgatcggac cagcgagcac
     1381 cgccggatcg ccggggccgt tgctgacgag cacggcacgc ggaacgttct cgaggatcgc
     1441 ctccgcgtcc cagtcgcccg gcacgaccat gacctcgagc ccacaagcgg cgaggcgcgt
     1501 aggtatcgag cgcttcgtgc cgaggtcgag cacggtgata ggcgcgcccg tcccgagtcg
     1561 gtacggctgc gccgtcccga ccgaacggcc gagcggccgg ccatcgatcg gcggttcggc
     1621 cagcgcgcga gcgtgcaact ccgccacgga cgcctccccg agcgcgcagc gaagcactcc
     1681 gccgtcgcga atcttccgaa cgagcgcgcg cgtgtcgacg tcctccaacg caaccacgcc
     1741 ctgcgacgcc agccactgct tccaggcggg tcgtgctctg cgcatcacga ttgcctcggc
     1801 gcacacgccg tctgactcga gccggctcga gtcgaccccg tagttaccga tcagcggata
     1861 gctgaaggcg agcacctgcg ccatgtagct cgggtcgctc gccgcctcct cgtagcccgc
     1921 catcgcggtc gtgaagcacg cctccccggc cgcgacgccg ggggcgccga cggcggcgcc
     1981 gccaaagacg gtgccgtcct cgagcacgag cttggctggt cggatcgttt gcatgatagg
     2041 tgctcaatcc gcataatacg cacagatgtt ctcggctgca ctagtaggtt gctccgggta
     2101 tgcaggtcag gaggcgctcg accgcgtcct ggcgcacccc gagctcaagg tggtcgcgct
     2161 cggctcggac tctcttgccg ggaagccgcc gtcgtcgctc gatctccggc tcaacggttc
     2221 gctgccgacg ttcgtcccga acgcggaggc gctcggaagc ggagctgacg ttctcttcct
     2281 ctgcctcgat cacgacgaag cggcagcact cgagccgccg ccagaagcgg tcgtcgtcga
     2341 tctctccggc ggtcaccggc tgcacgacgc ggatctctac ggcgactggt acggcttcgc
     2401 gcatcctttc cccagcgcgc tcgcggagtg gagctacgcg ctgccggagc tctatccgcc
     2461 ggcgggccgt ctgatcgcga atccgggctg ctactcaacc gcggcgttgc tggcgctcgc
     2521 gcccctcgcc gatgggatcg agccggaggg catcgtcgtc gacgcgaagt caggcgtatc
     2581 cggcgccggc cgcgcgctga aggcgagctc gcatgcgggg ttcgtgctcg agaatctctc
     2641 gccctaccga atcggcgcgc accagcatgc accggagctg gaacaggcgc tcggctttcc
     2701 ggtctgcttc gtcccgcacc tgctgccgat caggcgtggc ttgctcgcga cctgttacat
     2761 gcgcgccacc gacggcgcat cgcgcgagcg gctggaggac gcgtacgccg acagcgacgt
     2821 cgtgcgcgtc ctgcccgaag gtgtcgcgcc cgagctcaat cgtgtgcagt gcacggacac
     2881 cgctgaggtg gcgctcttcg aggaccgctc gaccggtacg acgatcgtag tctgcgcgct
     2941 cgacaatctc ggcaagggcg ccgccggcca ggcggtgcag aacgcgaacc tcgcgctcgg
     3001 cctcgacgaa accgctggct tgcggctgac cggggtgccc gtgtgagcgt caccgccgcg
     3061 aaagcattcg tcgcgagcgg ggtccactca ggcctgcgca gaagcgcgcc cgatctcgcg
     3121 atcgtgcgct cgctcgctcc cgcgaccggc gcggcgatgt tcaccgccaa ccgcatgcag
     3181 gcggcaccgg tcgtgatctc caaggagcat ctggagctcg ctgagccgca ggccgtcgtg
     3241 atcaattccg gctctgcgaa cgccgcgacg ggcctgcgcg gcgagctcga tgcgcgcgcg
     3301 accgccgccg aggcgggacg tctactcggc ctcgcggtcg aggagatcgt cgtgctctcg
     3361 acgggcgtga tcgggaagcg gctcgcgctc gacaagatcc tgcctgcgct ccggacggcg
     3421 accgattcgc tctcggaaag gggtggcggc aacgcagccg aggcgatcct gacgaccgac
     3481 acgcacgcga agcaggcggt tgcgaacggg ccgggcttcg cggtgggcgg aatggcaaag
     3541 ggctccggaa tgatccatcc caatctggcg acgatgctcg ccgtcgtcac gacggactat
     3601 ccgctcgagc ccggcgaaac gagcgctttc ctgcgcactg ccgtcgacga gagcttcaac
     3661 gcgatctccg tcgacggcga gtgttcgacg aacgacacag tcgtcctact cgccaacggc
     3721 gcaagcggcg cgacgcgctc ccctgcaagc gatgcggcgt tcgacgagtg tctgcgtgcc
     3781 gtctgcgccg acctcgcagg ccagatcgtc accgacggcg aaggcgcgac cttcacagcg
     3841 gcgatcagcg tcagtggtgc agcaagcgat ctggaggcga aggcgatcgc gcggcggatc
     3901 gcaacctccc cgctcgtgaa gacggcgctg ttcgggcgcg atgccaactg ggggcgcgtg
     3961 ctcgctgccg ccgggtcggc gccgttcaac ggcggctacg cgcagctcga tcccgattgc
     4021 gtcaccctcg acctcgcagg cgtgcgtgtc gtcgactcgg gcgcgccgca gaacgtcgag
     4081 ccgtcgctcg acggtgcggg ctgcacgatc gtcctcgacc tggcgctcgg tgaaggatcc
     4141 gccagctacc tgacgagcga cctctcgtac gactacgtcc ggatcaacgc ggagtaccgc
     4201 tcatgagtct cgtcgtcctc aagtgtggcg gcgcggtggc ctgcgagtcg gccgacacga
     4261 ttctgcaact cgccgacgcg ggccatgacg tgtgcgtcgt tcacggcgcc gggccgcaga
     4321 tctccgagga gatggagcgg ttcggcattc ccgtcgagtt cgtcggcggc cggcgcgtca
     4381 ccacggaggc ggggctcgca gtcgttcgtt cgtcgttcgc cgccgtcaac cgggcgctgt
     4441 gcaccgcgct cgggacgcgt gccgtcggtc tcttcggcga cgagatcggg ctccatgccg
     4501 tcgagatacc tgcgctgggg cgtgtcggcg atccgctgcc cagccggccg caggtggttg
     4561 tcgacgcatt cgtctccggc cacatccccg ttgtcgcccc gctgggtgtc gggccactga
     4621 atgtcaacgc cgacgatgcg gcggccgcac tcgcagtcgg gctcgaggcc gaccggctct
     4681 tgttcgtcag cgacgtcgcg ggcgtcttcc gcgacggctc cgtgctgacg agcatcggtg
     4741 cgggcgaggc cgaccgactg ctcggcgacg gcacgttcgc gggcggaatc gttccgaagc
     4801 tgcgcgccgc cgtaattgct gcacgcaacg gcgtgcgcac cgagatcggc gagacggcgg
     4861 tggtcgcgtg accgtcgttg ctcactctcc ggttcttccg gtctacgcgc gtcaagatct
     4921 gacgttcgtc cgcggcgagg ggacgacgct ccacgacccc gacggccgcg cctacctgga
     4981 cttcctggcc gggatcgccg tcgtggggct cggccattgc catccggcgc cgctcgcggc
     5041 cgcgcatgcc cagctcgacc ggctctggca cgtgtcgaac ctctactgga cggagccgat
     5101 ggtcgagctg gcgcagcggc tctcggcgcg ggcaggcggc gcgcgcgcgt tcttttgcaa
     5161 ctcgggtgcc gaggcggtcg aggcgagcct gaagtacgcg cgcaaggtaa ccggcaagcc
     5221 gggcgtcgtc gcgctgagcg gttcgtttca cggccgcacg atgggcgcgc tctcggtcac
     5281 cggccagccc ggcaagcgcg aggcgttcga gccgctgctg ccgcacgtgc agttcgcggg
     5341 cccgaacgac atcgcctcgc tcgaggccgc gatctctgcg gagaccggct gcatcctgct
     5401 cgagccggtg cagggcgaag gcggcgtgca cgtcctcgag gcggcgttcg tggccgccgc
     5461 gcgccgcatc gccgacgaaa ccggagcagt gttgctcttc gacgaagtgc agaccggagt
     5521 cggccggacc ggcgcgttct tcgcctacga gcacgtgagt gtccggcccg atgccctcat
     5581 gctcgcgaag ggtctcgcca acggcctgcc gatcggctgc ctgctcgtct tcgacgaggc
     5641 cccggagggc ttcgccgcgg gagaccacgc cagcacgttc ggcgggaacc ccgttgcgtg
     5701 cgcggccgcg agcgccgtct gcgatgagtt gacggatgag ctgctcgcaa ccgtcaaggc
     5761 gaatggtctc ctgctcgccg acggtctcgc ggcgctgccc ggagtccgcg aggtgcgcgg
     5821 cgccggcttg ctgctcggcg cggagctcga ccgcccggcg gcagatgtcg tcgcagcgtg
     5881 cacggagcga ggtttgctcg tcgggacggc gggcgcgaac gtcgttcgcc tgaccccgcc
     5941 gttgatcgtc tcgaccgctg agatcgaaga cgcactcggt gtcctagagg aggtgctcgt
     6001 atgaggaagt tcgagcggca aggcgtgatt ctgcgcctcg tatccgaaca ggagctctcg
     6061 acgcagacgg agctggcggc tgcgctccac gaggcaggct tcgacgccgt ccagaccacc
     6121 gtctcgcgcg acgtcgcgga gctcgggctc gtcaaggtgc gcaacgagga agggcggctc
     6181 gtgtacgcac ctcccggcgc ggccgatctc tcgcgcctcg gcgagctcgc ctcggccctg
     6241 cgccgctggg cgctctcgat cgtcccgacc ggcaccatcg tcgtgatcca gaccccgccc
     6301 ggccacgcga acgcgctggc acgggcgatc gacggcgcag gcctcgcgga cgtcgccggc
     6361 acggtcgcgg gcgacgacac gatcttcgtc gccgcgcgcg agggcagcgg caccgcgctc
     6421 gcagaccagt tgcaacatca cctggaagga gaaagccagt gaacggcatc gccgtgctcg
     6481 cctactcggg cgggctcgat acgagctgcg cgattgcgtg gctgaaggag gactacggct
     6541 tcgacgaagt ggtcgccgtc ctggtcgacg tcggccagga ggcgagcttc cacccggcca
     6601 tcgagcgcgg caagctcgcg ggagcggacg acgtcctgct actcgaccgc aaggaagcgt
     6661 tcgcagacga gcaggtggcg aaggctttga tcgcaaacgc gttgtacgaa gggaagtacc
     6721 cgctggtctc cgcgctctcg cggccggtga ttgccgaggc ggtcgccgag gtcgcggtcg
     6781 agctcggcgc cgacgcggtc gtgcacggtt gcaccggcaa gggaaacgac cagctccgct
     6841 tcgagcttgc cttcaaggcg aagtacccgg gcgtgaaggt gatcgcgccc ctgcgcgacc
     6901 gcgtgtggac acgtgacgcc gagatcgcct acgccgagga acgtggcatc cccgtcgagg
     6961 cgaaggcgga atcgccgtac tccgtcgacg acaacctgtt cggccgcgcg atcgaggcgg
     7021 gaatcctcga ggatccctgg acggcgcccc ccgaggacgc gttcctcctc accggggatc
     7081 tctcggtcgc acccgctccg accgacgtca ccgtctcgct cgagcagggc cttcccgtct
     7141 ccatcgacgg agaagagctt ccgctgttcg cgctcgtagc tgtgctcaac gagcgtgcgg
     7201 gcgcatacgg catcggccgc atcgacatga tcgagaaccg cgccgtcggt atcaagagcc
     7261 gggagttgta cgaggcgcct gcagcgttga cgctgatcgc cgcgcaccgc gcgctggaag
     7321 agctcgtgct gacgaaaggt gagctcgagg ccaagcgcga gctggaaccg aaatgggcga
     7381 agctcgtcta cgacggcggc tggttcgcgc cggtccgcgg cgcgtatgac gcgttcttcg
     7441 cggcgacgca ggagctcgtc acgggcgacg tgcgcttgtc gctccagccg ggagcggccg
     7501 tcgtcaccgg ccggcggtcc gaccatgcgc tctactccga atcactcgcg tcgtacggca
     7561 tcggcgagac cttcccgcac cacgaggccg caggcttcat cggcatcaca gcgctggaga
     7621 cgcagctcgt ggccgcgcgc acacaggtgc aggtcgcatg acgctctggt ccgggcgcgt
     7681 cgagagcgac gtcgcgcagg cggtctggga cttcctgcgc gcggacgacg ccgagttgct
     7741 cccgtacgac tgtctcgcca cgcgtgtgca cgcccgtcgt ctgcacgagg gagggctgct
     7801 ctccgacgcg gagctcgccg acgtcgagac ggcgctcaac tggatcgcgg tcgagggggc
     7861 ggtcgagccc gaggacgagg acgtccattc cacgatcgag cggctgctcg gcacgatcgg
     7921 ccgcaagatc cacgccggcc gctcgcgcaa cgatcaggtc gcggccgctc tgcgcctcta
     7981 cgtcgccgac gcgtcgctcg aagcggtgac cgcgatccgc ggcttcgttg tgtcggttct
     8041 cgcccgggcc gaagaagagg ccgagacgcc catgcccggc tacacccatc tgcagcgtgc
     8101 tcagccgatc acggtcggac accacctgct ggcctgggtg gagatgctcg accgcgaccg
     8161 cgaccggttc cgtctcgccg cagaggcagc ccgcccctgg ccgctcgggg ccggagcgct
     8221 ggccggatcg acgctcgagc tgccgccgcc tcccggagcg cagcttcgta actctctcga
     8281 cgcggtgggc gaccgtgact tcgcgctcga ctatctctat gcatgcgcga tgctcgcgac
     8341 gcatctttcg cgcgtcggtg aagagatcgt cctgtggtgc acgaccgagt tcggtttcgt
     8401 ccggcttccg gaggaagcgg cgacggggtc gtcgatgatg ccgcagaagc tgaatccgga
     8461 cgtggccgag ttggcacggg gcaaggccgg cgccgcgatc ggccgcctca ccggcttgct
     8521 cgcgacggtc aaggggcttc cgcttgcgta caaccgcgac ctgcaggagg acaaggagcc
     8581 ggtcttcaag gcgcggcgtg atgttggcgg tacgctcgcc gcactcgagg tactcgttac
     8641 cggcatcgaa ttcgatcacg agcggctcgc tgctgcgtgc agcgatccac tcgtgtcggc
     8701 gacggacgct gcagaagccc ttgtcgcgga gggagtcccg ttccgtgacg cgcacgagca
     8761 cgtcgcgggc caggtccgcg aaggaacctt ccaggcgccg gagttgcctc gccctcgcgg
     8821 cgcgccgggc ccggccgacg tgtacgcagc ggtcgccgag gcgcgcgccc gcttcgacgc
     8881 ctaggctgca tcagttcgcg aacacgcatg gcgcccgcga cgatcaagcg agacgaaaga
     8941 tttcctcgag cggcgacggg ccctgatcct ggacgcgttc ctccagcagg tcggccatcg
     9001 cgtcctgcca cagtgcattg acttcctggg cggcgaggta ggtttcggta cgcgcgagat
     9061 cgtcggtctc gaaatacccg aacatctcgt tccccgcgcg gaagatcgtg tagttccgga
     9121 ttcctgcgct cttcagcgct tcgagcatct ccggccagac ctcgcggtgg gcgagaacgt
     9181 actcgtcaac ccggtccggc ttcacccgca gaacgaatcc caaccgttgg gtcatctcag
     9241 tctgcctcct tcaggatcac gacgatgagc gtccgcggtc cgtggacgcc ctcgactcgt
     9301 tccagttcga tgtcggaggt ggccgacggg cccgacacga gcgtgagagg tcgacgctcg
     9361 cgaccgcgaa gcgcagcgaa cgcttcgggc accgtctcca cgatctgatc tgtgcggacg
     9421 acgcagatgt ggacgtcagg gacgagcgtg agcgcgcgcc tgccttccat ctctccgccg
     9481 gagaggatga gcgtgccagt ctcggcgatc gcgacggtgc accccgtgat gacgccgtcg
     9541 agagcatcaa gctcgtgcgt gctcaaaccg tcgtcttcga ccgagttggg cggtcgccac
     9601 ttctcgggga ggccccgcgg gacggcgata cggccgtcgc gcgcaagctc gccgagcagg
     9661 tcgcgcagag ccgttgccgt cgtccgctcg acccgagcgc gatagtcgag aaggcgctcg
     9721 ctcaggagcg cgacccggcc cgcatcgtcg aggacgcccg tcgtgcgata tgcacgtgcg
     9781 acggggtcgg gtggtgcgcc ttcgctgatc gcggcgcgcg tccgcgccag aatctcgtca
     9841 cgcgcctcgt tcacggctcc accactcccg gaaggtctcc ttcgcaaacg gcttcagatc
     9901 gcgagtctgc gtccatgcgg cgagcgggcc cggcacacga cgggccggtc tctcgccggc
     9961 gcgcgcgagc ttctgcgccc actcgtaaag ccgtgcgttc ccgaagatac gagcgagcgc
    10021 gcgcatggcg atccgctccg cgacatcctt ctccgtctgc gctcgcagat gcaggagtac
    10081 gctcggaatg tcgatcttca cggggcagac ctcgtagcac gcgccgcaca aggtcgatgc
    10141 gtacggcaac gacttggcat tcgcgacacc gcgcagctgc ggcgtgagga tcgcgccgat
    10201 cgggccgggg tacaccgagc cgtaggcgtg gccgcccgtc cgcgtgtaca ccgggcagac
    10261 gttcaggcac gcgctacagc gaatgcagtg gagcgcttgc cggcccacct cgtcggagag
    10321 cacgcgggtc cgtccgttgt ccagcaggac cacgtggaac tcttgaggtc cgtctccgat
    10381 cgtcacaccg gtccaaagcg acgtgtacgg gttcatccgc tcgcccgtcg aggaccgcgg
    10441 cagaatctgg aggaagacct cgaggtcggc gaacgtcggg acgagcttct cgatcccaag
    10501 cactgtcacg agcaccggcg gcagcgtcgt gcacatccgc ccgttgcctt ccgactcgac
    10561 gacgacgacg gtcccggtct ccgcgacacc gaaattcgcg ccgctaactc ctacctttgc
    10621 ctcgaggaac ttctcgcgca ggtaaagtcg cgccgtttcg gcgagctcgc gcggatcgtc
    10681 cgaaagctct cgcccgtccg caatcgtgcg catgaacaga tcgcggatct cggagcgatt
    10741 gcgatgaatc gccgggacga ggatgtgcga cggccaatcg ccgtcgagct gcagaatcag
    10801 ctcggcaaag tcggtctcga tcgcgcgcac tccgccggtg gcgagcgctt cgttgagacc
    10861 tatctcatct gtcgtcagcg atttcacctt gacgacctcg gtcgcgtcgt gcgcacgggc
    10921 gacatcgagc aggatcgcgt tcgcctcggc tgcgtcgcgt gcccagtgga cctggccgcc
    10981 cgcggcttgc accgactctt cgaattggag cagatactcg tcgagccggc gcatgacgtc
    11041 ttccttgatg gcgcggcccg cttcccgcaa ctcctcccag tccggcacct cggcgacgac
    11101 gcgcgcgcgc ttgtcacgaa tcgtggtcgt cgcgttgcgc agattgtggc gcagctggct
    11161 gtcgccgaga gcgatccgtg cggcctcggg aaacgacgtc acgaagcgag tatctcggcg
    11221 aggtgcatcg tgcggatccc tgttccctgc cgcgaaaggc cgccgccgat ctgcatcagg
    11281 cacgacgcgt ccacggccgt gacaacctcc gcaccggtcg caaggacgca ccggcacttg
    11341 tcctcgagca tcgccgtcga ggtgtccgcg tttttgacgg caaacgtccc gccgaaacca
    11401 cagcattcgg ccgcttccgg aagctcgacg agctcgaggc cacgcacccc gcgcagcagc
    11461 cgcagcggcg ccgccccgac ctgcaccacc cgcagcgagt ggcaggtcgg gtggtaggtg
    11521 acgcgatgag ggaagtacgc ggcaacatcc tcgacgccga gctgcccgac caggaactcc
    11581 gacagctcaa agactcgcgg tgcgatctcc gggctcagct cgcgcactgc cccgacacag
    11641 gacgacgacg gggagacgac ggcgtcgaaa tcgccgaaga tccgggcgaa cctccgtgcg
    11701 agcgcctgcg cctcatcgag atacccgctg ttctgatgga gctgcccgca gcaggtctgc
    11761 tcgcgtggga acaccacgtc gtgcccgagc cgctccagca ccgtgacggc cgccctcggc
    11821 gcttcgggga agagcgtgtc gcccagacac gtgacgaaga gcccgatgcg cattcgcgaa
    11881 ttcaccgagc tgagctactc cacgacagcc agctcgagac ccaacaggtc gccaacctta
    11941 cgcagacgcg caacctggtg cccgactccg agagccacat gatgtgtggg gccctcggcg
    12001 caccaagcgt cgaaaaacgc ggttggcgcc gccgcaaagc ggatccgaga gttcgtgttt
    12061 ccgattcgga acgttggccc cgcaatcgat tcgccctcag ccgcgatcaa cttcaagcgt
    12121 ccatcggcgg tctgtgtgac gccgaggatc gtcaccggtc cccggcgaac cttcatctcg
    12181 atcgataggc cagcgcccgc tttaccgtga tagagcttga gagcgcgggc gacgggtcga
    12241 ccttcggcga tcgccaggtg gccggggcca tcgtgcccca tcaggacaaa cgcatcgtcg
    12301 aagtcgaggg cgtagaactc ggtgtatgag ccgcctgcgc ccagccggtc gagcaagagc
    12361 attccgaggt tcgtcttcaa gtcgccctcc cccgacgcgg gaacgccgcg cgcggtgagg
    12421 agcgaattgc ccacgatcag cccggccgtg acccgctcag cggcgttgcc gccgatgccc
    12481 cggtagtagt acgtcagtcc gtcgagctcg aagtcctcga cgaggcggtc caggccgacc
    12541 gcgacgcggg ccgaccaatc gaagatctcg ggcgtgatgt cggccgcgat cgggtcgacg
    12601 ccggcatcgg cgaggtcgaa ggtctcgcgg atttcctccc ctttgcgctc gatctcggcg
    12661 gctttcgccg actcaacgcg ctcgacgagg tcgtcgatct ccagcacctc gacatgcgcc
    12721 ccggtttgcg cctgcacctg cgtgaagtcc gagtacatgt cgagcatccc cggataggta
    12781 tggccgagga agccgatgcg ggcagtccgc aggctgtgca ccgcgtgcgc cgcgtcaagc
    12841 cactcgtgca gtacgtccca tgccggatcg ccgtccagaa gcgtcccggt aactgtcctg
    12901 tagtggatcc gagcacgcgt cagtgcgccg gcgatctccg gaacgcagca tgcggagcag
    12961 ttggcgagcc actcgcctgt cgtcattcct tcgtagtcga gcgtgcgggt cggctgcagg
    13021 ttcaggatga caacgggcgc cccgactgcc tgcacaatcg gcagcacttg cgacgacgtc
    13081 gcgtacgtcg ccgggtagac catgacgaga tcgacccgag cggcggcgag attcgcgccg
    13141 gcgtctcgtg cggccggcgc cgtgtccacc agccccgccg acacgacctc tgcgcccatc
    13201 gcctcgagcc gcgcctggat gtcccgctga tagccctcga gtcgctcccg cagcccctcg
    13261 aactggggcc agtacgcggc gaggccgatc ccaaagatgc cgacacgcgg cgcggcgctc
    13321 tgtttcactg cccttccccc tgcgcggcgg cggcagcgtg gcggcgggct tcagcgcttc
    13381 gcagccgagc gcgcgcgcgc ctatagagct cgccgccgtt cgggatgatg acactcgcaa
    13441 tcaggagtgc gccgacgacg atgttctggt cctgcgcagg tatgaggtcg gccgtgagcg
    13501 ccgtttgcag agtcccgaga atagccacag cgacgatcac acctgcgatc gtgccgcgac
    13561 cgccgaagat cgaaatgccg ccgagcagga cgatcgtgac cacgaggagc tccataccaa
    13621 cgccggcgtc gtaacgggcg gatgcgaaac gaagcgtcca cagcacacca gcgaaaccgc
    13681 tcagcgttcc ggagaggacg aagagccaga acttgatccg cttcacgcgg atgccggcga
    13741 agaaggctgc ctcatggccg gcgccgatgg cgaagatcgc gcgaccgagc ggcgtggcgt
    13801 gcagaacgat gccgaagacg acggcgagcg cgaggaagaa cgcgatcgaa tacgggatgt
    13861 gcgtgtgtgg gatcggaacg acaccgatgc tcgccaggcc ggccggaaag ccgccaatcg
    13921 tgtccgtcgg caggattccc tgcgcgatgc cgcgatagag cgtcagcgtc ccgattgtca
    13981 ccgcaagcga agggagaccc atgcgcgtga cgagaaagcc gtttaccgca ccaaggaatg
    14041 cgccgaggac gacagccatg atcatcgccg gccagatcgg ccaaccgtga gtgaagagct
    14101 cggccatgaa aactccgctg aggcctagca ttgacgcgac cgagaggtca atctcgccgg
    14161 tgacgacgat cagagtcagc gggagggcca tgatcgcgac ttcgccgacg ttcaagctga
    14221 tgtagaagag gttcgtcgag tgcaggaagt acggcgagac agcggccccg taagcaatcg
    14281 tgccgccgag gagcacgagc agcaggactt cccagcgcag gaacacgccg atcccggtgc
    14341 gcctcggtgc ttccgtcccc ggaatctcct cgtgcggctg gaaaccgagg tcagacacgt
    14401 tctctcctcc gcgtccgcag cgcgggcgcg acgcgcacgg cgatcaaccg gtcgaaggcg
    14461 atcgcaccga cgagcagccc cccggcgagc gcttgactcc agaacgacga aacgtttacg
    14521 acgacgagtg cgctgttgat cgtgttcagg aggagggcgc cgagcgcggc tccgagcacg
    14581 ctgccgctac cgccgaagat tgcgacgcct ccgacgacga ccgcggcaat gacctgaaac
    14641 tcgtagccgg cgccggcgat cgcgtcgacc gacccgtagt acgacagcca cacggccccg
    14701 gcaacgccgg cgatcgcgcc gctgagaacg aaggcgagaa acacacgcgc gcccaccggg
    14761 atgcccgcaa cacgggcagc atcgggatcc gagccgatcg cgtagaagtc acgagccgtc
    14821 cgaaagctgc gcattgcgta ggttgcgatc gcgacggcga cgatggcgat gattccgagg
    14881 tacgggacgc cggcaaccgt gccgtagccg atcttgttga aggtgttggg caacatcgag
    14941 gcgttgacct ggttcccgcc ggcccacgca ccgtcgatcc cgcggatgat gtagagcgtt
    15001 ccgagcgtga cgacgaggct cgggacgcgc gcgaccgtca cgatcaggcc gttgacgatc
    15061 ccgcacccaa cgccgattcc gagcgcgagc aggaagacga gcgcgagcgg gacatgcgga
    15121 tggtgcacgt agagctcacc ggacgcatat gcggagatgc cgagcaccga tccgatggag
    15181 aggtcgacgt tgcgcgtgat gatgacgatg gtctccccga tcgcgagcag agcgatgatc
    15241 gatgcgcccg aaaggatctg ctgaacattc gtcgcgctga ggaagaggtg attgtcgatc
    15301 gtcgttgcac cgaccaccag cacgagcgcc agcacgatgc ccagctcacg gaagcggaac
    15361 aacacctccg caaacgcgcg cgagctggcg tacggctgga cgaccttctg ctcttcggcg
    15421 accgcggtca tgccgcttcc tcgaccatcc cggtagccgc gtgcatgagc gcgtcctcgg
    15481 ttgcttcggc gcgcgtgaac tcgcgcatga cgcggccctc gaagagcacg atgatgcggt
    15541 cggccatccc gagtacttcg ggaagctccg aggagatcat gagcacggcg accccctgcg
    15601 cggcgagctc ggagagcagg cgatgcacct cggccttcgt gccgacgtcg atgccgcgcg
    15661 tcggctcgtc gacgatcaac agcgtcggtt tgcgcgccag ccacttcgcc agcacggcct
    15721 tttgttgatt gccacccgag agtgtccaga cggggttcgt caacttgccg tacttcagtt
    15781 gcagccggag cgcccagtcc ttcgcgaagc tccgctcggc gccgcgcgga atgagtcccg
    15841 cacgttgaat attgtcgagc gaggcaagcg caatgttgcg ctcgatcgaa aagtccatga
    15901 cgagcccctg ctgacgccga tcctcgggga cgagcccgat gcccgcgcgc atggccgctg
    15961 tcggcgatcc tggccggagc cgcctgccgg agatctcaat cgcccctgcg tcccaacgat
    16021 cgattccgaa gattgcgcgc gcgacctccg tcctgccggc gcctacgagg ccggcgaggg
    16081 cgacgacttc accgctccgc acgttgaacg agacgtcggt gaacacgccc tcccgggtca
    16141 gccggttgac ctccaggacg actgggcccg gctcggtcgg cactttcggg aacagggcgt
    16201 ccatgtcacg gccgaccatc gcccggatca cggattggat cgtcagctcg tcgatcggct
    16261 tcgtcaggac atggcgaccg tcgcgcatta ccgtgacgcg ctgagagatc tcgaagatct
    16321 cgtccaggcg gtgcgagacg aagagcaccg cgttgccccg cgaccgcaac gactcgacga
    16381 ttccgaacag ccggtcgacc tccgtgccgg ccagggcggc ggtcggttcg tccatcacga
    16441 tcacccgcgc gttcgacgtc aacgctttgg cgatctcgac tagctgctga tcggcgatcg
    16501 agagcccgcg cgccaggcgg tcaggatcga ggcggacgcc gagctgctcg aagacggcgg
    16561 ccgcctcgcg ccgcatgcgc ctaccgtcga tgcggcgcaa gcgcttgagc ggctgcatgc
    16621 cgacgaagat attctcggca acgctcaggt cggggaagag cgtcggctcc tgaaagatga
    16681 tcgcgatccc ggcggcctgc gactgcttgg cgttgtcgaa gatggcctcc tcgccgtcga
    16741 gcacgagtcg cccaccctcg gggcggtgga caccggcaag gatcttgacg agcgttgact
    16801 taccggcacc gttctcgccg acgaggccgt gcacctcgcc gccgaacagc tcgatgtcgc
    16861 cgtcctcgag ggcgtggacg gcgccgaagc tcttttgagc gtgctcgaga gcgagcactg
    16921 gttcgggatg ggttgtcgtc aaacggtcgc agtgaggggg gcgttcacga ctcggcgccc
    16981 ccctcgtctg cgcctcctag aatttgaact tgtcgacgtt cttgatggtg aacgtgaacg
    17041 gcggtccgag aatcacctgc ggccgcttgt ccgggccgtt gatgatcgtg tacttgccga
    17101 gcttgccggc cgtgaacgtc tgaccgacct tgccggtgac cttgccgtcc gcgaacgccg
    17161 caatcgcgta gcccgcgaga tacccgaggt cttcggggtt ccagagctgg aacgcagtca
    17221 ccgtcttgtc atgtacgtac ttcttcatct ggctcggcag gccgaggccc gtgaggacga
    17281 ccttcccctt gtacttcgag gtggagaggt actgggcggc cgaggagatc ccgacggtcg
    17341 tgggcgactc gattcccttc aggttcgggt acgcctgcaa catgctcacc gtcgcctgcc
    17401 gcgattgagc cgggttgtcg ttgccgtagt agatcttcac gagcttcatg ttcttgtaga
    17461 ccggcttctt cagctcgagc ttcatgtact tgatccacgc attctggttc gtggccgtcg
    17521 acgccgccga cagaatcgcg aactcaccct tgtacttcat cagcttgccc aacagcttga
    17581 tctggctccg gccgatcgtc ggcgtgtccg cctggttgat gaagagctgg cggcagttga
    17641 cgtccgagtc gaacgcgatg accttcgtgc cctgcgcctt cgcctggttg agcgccggac
    17701 acacggcctg cgggtcgtta ccggcgatga cgatgcctgc cgcatgcgcc tggatcgcgg
    17761 cctggatcgc cggttgctga gccgcagcgg tgtcctccgt gccagacgag acgacctgct
    17821 tgtgaccgag cttcgtgagg gcgatcttgc cgccccggtc ggcgatgacc tcgtacgggt
    17881 tgagcgtgtc cttcgggatg aagtaaaacg tcttgcctgc cgtcgagcgg ctgttggccg
    17941 ccagcgcggc cgtcacggcg acgcctgcaa ctagcaccgc cgccacgata atcgtccatc
    18001 tgcgcttgtg cattgatggt tcctttcgcg tgcggattga ctgacgaatc ggttggatcg
    18061 tgcgcctcct tcgcactacc tggggtaggc ggcggtgaca ccgccgtcga cgttgatgac
    18121 gtttccggtc gacttagccg agcgggggcc ggcgagaaag gcgatcgcct cggccacgtc
    18181 ctccggataa acgctgactc cgagcttggt tcgcccgctg tagaagctct ggaggtcatc
    18241 ttcggtaacg ccatacgtgc tggcgcgttc cgccttccag tccgacgacc agatacccga
    18301 gccctggatc acggcatctg ggttcacggt gttgacgcga attccgtgag gtccgccctc
    18361 ctccgcgagg caacgcgcga gatgcagaga ggccgccttt gcggatgaat acgctgcggc
    18421 gttcgctcca gcgacgagcg cgttcttcga tcccacgaag acgattgagc cggcgcggtc
    18481 ctgcttgagc aggacgcgaa acgcttcacg ggcgcacagg aagtagccgc gtgcgaggac
    18541 cgcgtaattg agctcccact cctcgagcgt cgtctccgtg accggcgcgc tcgtcgcgag
    18601 cccggccgag gcgacgagga tgtcgagccc tccatactcc agaaccgtct gctccatcat
    18661 ctcggcaacc gcgttctcgt cggtgacatc gacgtgggcc gcaaccgcac ggcggaggcc
    18721 gcaagcaccg actatctcct cggcaaccgc ctcggctccc tcgcggttga ggtcggcgac
    18781 gaccacatgc gcaccaaggt gggcgagcaa ccgggcggtc gcacgcccga tgccgctcgc
    18841 tccgccggta atgactgcaa tccggcctgc gagctcccga ggcggcgggg cctgcgcgag
    18901 cttgtaccgc tccaggggcc agtactcgat cgcgaatgcc tcggcctcgg tgagagagcg
    18961 gaatccgccg atcgcattgg ccgcgtcctc cactgcgatc gcgcgatggt agagatcgcg
    19021 agccgtccgc gtccgcgtcg cgtcggcgcc agtggtgacg atgccgacgc cggggatcag
    19081 aacaacccgg ggacctgccg gatccatcgg aaagggtcga ctctcatcgc tgacgttgcg
    19141 ttcgtaatac tcgcggtacc aatccacgta cgcggcgacg ccgtcgcgga aagcatccgt
    19201 cagctccgca gctccagcgc tcgacggatc gaactcgatc gcgagtggct tgtgtttcgt
    19261 gttgatcaaa tgatcggggc acggtgcacc gacctgactc acatcggggg cgcgcaccga
    19321 agaagcgaac gcgaccgcct cgggactgcg gtcgacttcg agcacgacgc cgtccgcgtc
    19381 ggtcaagagt gctccgcgga gtgcgggaag cgacgccagg aggaggaact gagcgtcttg
    19441 ctcaccgcac tccgcgacct tcgggcctcc cagcccgaag cggccggcac cggccttctc
    19501 gattgcgcga gcggcacggg agacgaactc gatcgtgcgc cggtagctct cttcacccga
    19561 ctccccccaa gtcaccaacc catgtttctc gagcagcacc gcgtgggccg atgggttggc
    19621 gtccagcagc tcggcgattc gacgtgacat gtcgaagccc ggacgctgat agtcgagcca
    19681 gacaacctct tcgccgaacg tctcttcggc caacttgcgc ccgttcggtg acgacgtcaa
    19741 cgcgatgatt gcgtccgggt gagtgtggtc gacttggggg gcggcgatga acgcgtgtaa
    19801 gagcgtctcg atcgatgggc gtggctggtc ggggtcgagt gccgagcggc gtagaaagcc
    19861 gaccatcgcc gtgtcgtcca tctcctcgcg ccgacgaagg gggatgatct cgtcgagccg
    19921 caggacggcg aatccactag gggcgatcga cgccaggtcg gtgccagagc ctttcatcca
    19981 gagcacgcgc tgctcgcggc ccgcatggtc gaccgccgtg cccttcgagg acgtgttgcc
    20041 accaccctgg ttggccagag cacggtccga gccgaggaga ttcgagcgat agacgagcgc
    20101 gtcgagggag ctcaaacctg cggagcttgc gtcgtcccac cgatcctccg gcgcgggaat
    20161 cccgccgagc atctttgcga cgctcataga ccgacctcca acgtcggcaa ggcgacggcc
    20221 ctcgcgaacc gctcgcgcgc ctctcgccag accgccgtgt cctgcggctc gtagatcgtc
    20281 ggtgcgaacg atgcgcgcac gatctcgcgt ccttcgtcga tcgacgcaag ctcgccgagc
    20341 gacatcgcct ggacgaggaa gttgcccagg agggtcgcct cttccgggcc ggccaagaca
    20401 gggagcccgg cggcgcctgc ggtccaacgg cagagcaggt cgttgcgcga accaccacca
    20461 acgagatgaa tttcctccgg agcggtgccg gtgacggacg cgagcacgtc gatcgtttgc
    20521 gcgtgcttga gcgcgaggct ctcgaggatg cagcggacga ccgcaccggg ctcgaatggc
    20581 tcgggctgac ctgtgtgagc gcagaacgcg cgcacacgcg ccggcatgtc gccctgatcg
    20641 gagaaggccg gatcgttcgg ctcgatcagc gaccgcagcg cgggggcttc cttcgcgagc
    20701 gcgacgagct ggtcgaacga atagtcatgt ccctcgagcg cccaggcgcg acggcactca
    20761 tgcagcagcc acagtccggt cacgttacgc aggagccgga acgtagagcc aacgccgccc
    20821 tcgttggtca ggttggcggc gaaacttgca tcgctgatca caggctgctg aacctcgagc
    20881 cccacgagcg accaggtgcc ggcgctgatg aacgcagaac tcggccgacg gaacggcact
    20941 gcggcgatag ccgaaccggt gtcgtgggtc gcgacggcga cgacaagcgc gccaccgagc
    21001 ccggtgtccg ttgccaccct ctcgctaagc cttccgagct tcgtccccgg ccgcacgacc
    21061 tcgggcagca accgcgtggg tacgtcgagc cgctcgagca cgtcaaccgc ccacgtcccg
    21121 gtgcgcgggt cgaagcactg cgtcgtcgtc gcgttggtga gctcagacgt gcgcgcaccg
    21181 cagagccagt agtgcatcag atccgggatc aacagcagcg tctcggcgac ctcgagcgca
    21241 gggtcgtgtt ctgcagccat agcgcccagc tcgaacaccg tgttgatcgg gatgagctgg
    21301 atgcccgtgc gctcgtagag atcccgcgcg ggcacctccg cgagcacgcc ttccaccgcg
    21361 cgcgcacgac ggtcgtcgcg gtagtgaacc gggttctgca aaagtcggcc gttctggtcc
    21421 agcaggccga agtcgaccgc ccatgaatcg accgctaccg agtcgactgc accggcctcc
    21481 cgggccgctg cccgcagacc gtcggttacg tcctggtaga gacggaggat gtcccagttg
    21541 agcgtgttcc gcgttctgac cggaacgttc gggaagcgat gcaccttcga gacgctcaga
    21601 cgctcgccgt cgaaacggcc gacggccacc cttccgctct gagcgccgag gtcgattgcg
    21661 gcgaacgtcg ccatcagccg ccggcctcgt cgttgcggac tgcgcgacga aggtcgcgcg
    21721 gccggagcgg cagcggctct cgcggacccg ggtcgacggt gaggacgtcg acgccggccg
    21781 cacgccacac cccgacctcg gcggcgggcg cgctcgaatc cgtcacgatc ccggccaggt
    21841 cctcggcccg gacgaatgcg agcagtgcgc tgcgatgcca cttggtgccg tcgaagattc
    21901 catacacctg ctcgcacgca tccgccatct cctgcttgat gctgacctcg tcaggattga
    21961 ggtccatcag gcctcgagag atgctcaacc cacgcgcgcc gagaaagcct ttgttgatcc
    22021 gcgtcgtccg caggacgtcc gcgccgagat ccccgacgag ggacatcgcc gagagccgca
    22081 gcatcccgcc tgtcacgagc accgtaatgc ccggggcatc ggcgagcgcc gtcgcgacga
    22141 gcaggccgtt cgtgacgact accaactcgc gcttggagcg aagctcgagg gcgaggtagt
    22201 aggcggtcgt gctcgcgtcg agggcgacag cctcgccctc gtcgaccatc gcagccgccg
    22261 cgcgggcgat cgcgcgcttc cgctcgacct cgaggcgtaa gcggtgatcg aaccccactt
    22321 ccgactgccc cagctgcaag gccctgacac cgccacggat ccgcgcgacg aggccctgct
    22381 tggccagcgt cgtcaggtcg ctgcgcacgg tcacctccga cactgcgaag gcttccgaaa
    22441 gttccgcaac cggtagataa tcgcgactct cgagcaggtg caagacgtgc tgcatgcgct
    22501 cctgttgcgt caaattgcga agatcttctg aggcgccgat ggcgcgacct tactcccgcc
    22561 cctgcgcgga agtcaagaga tactggttgc tgacttacga aacaatccga aacgaaagcg
    22621 aactcgtaat tcggccgcgg atccgtcaga ctcgccgctg atgaacggcg acgccatgtt
    22681 ctcgcgcctc gatgcactcg agatcgagac cccgtcgtgg gggtacggaa actcggggac
    22741 gcgcttccac gtctacccgt ggccaggcgc cgcccgcacc gtctgggagc ggatcgacga
    22801 cgcagcactc gtccacgagt tggccggctg ctgcccgagc gtggctttgc acatcccatg
    22861 ggataccgtc gacgattgga gcgccctcgg cgactacgca cgcggtcggg gcatccggat
    22921 cggtgcaatc aacccgaacc ttttcgggga cgactcctat cgtcttggca gcctcgccaa
    22981 ccccgatcca gccatcaggc gccgcgcact cgagcactgc atcgaatgcg tcgagatcgc
    23041 gcgacagctt ggttcgacga tcgtcagcct ctggctcgcc gacggaacga actatccggg
    23101 gcaagacgac ctgcgaggcc gtcatgctcg gctcctcgca gggctcgaag agctctacgc
    23161 ggttctatcc cccaaccagc aattg
//
LOCUS       DASVEU010000043         5226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_224523,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000043 DASVEU010000000
VERSION     DASVEU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224523"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF225_01690"
     CDS             <1..310
                     /locus_tag="VF225_01690"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7143995.1"
                     /translation="WAFHVFLRHVDRGLNLQESIDAPEFHTDHLISSFYPRGFVPRSL
                     DLEARFGSAVIDDLRARGHEVTVTPDWSLGRVTAVQKADGQLRAAANPRGMQGYAAGR
                     "
     gene            361..2769
                     /locus_tag="VF225_01695"
     CDS             361..2769
                     /locus_tag="VF225_01695"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7143996.1"
                     /translation="MESLYEPEGVEERWQRTWETEGHYNADPDPSRESFVIAHPPPNV
                     TGDLHMGHALQLSLADTIIRTRRMQGYNALFQPGFDHAGISTQNAVEKHLAQEGKSRQ
                     DLGREAFVALVWEWLREYGGKIMLQFRRIGASMDYRRERFTMDDAYVHAVMRFFVHLY
                     GKGWIYRANRIINWCPFHQTALSDLEVAHVELDDALTYARYPLADGGGHITIATVRPA
                     TILADVAVAVHPEDDRYRNLVGREVIVPVVGRRVPVIADERVEREFGTGALKVTPGHD
                     PLDYEIGRDHGLPELTVIGPDGRMNEEAGELAGLTQEEADQRVLDWLKEHGQLEKRES
                     HRHTVALCDRCHSRIEPRISLQWWCSMEELRQPALEALRSGRVRYHPESQHRFAIKSL
                     EDAPDWNISRQIWWGHQLPVWECPDGHITVAETEPDACAQCGSHELTRSTDVLDTWFS
                     SALWPFATLGWPDDTEDLRTFYPGDLNTTARDIIRLWENRMIFSGLEMMGDVPFHDVV
                     IHSTVHAPTGGRMSKSLGTGMNPLAAIETYGADATRYGLMKMASSQDVRFSEGAIEEG
                     RKLANKLWNVARLILQASEGAAPEERPRTVEERWILARLSQTQRELERLLGEFDFSHL
                     VHELYHLTFDDFCDWYAEAVKGRLYEADEDARATAVAALERLLKLLHPALPHVTEEIW
                     SNLPARQTRLIVAPWPVAGSEADAGALTAVQEAAVMFRRSGVLPKLEGEERRIFDAVV
                     KPGEGAGDGNAAAEIERLHKEIARAEGMLANDRFTQSAPAEVVEAEREKLARYRRELD
                     AISS"
     gene            2914..4023
                     /locus_tag="VF225_01700"
     CDS             2914..4023
                     /locus_tag="VF225_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7143997.1"
                     /translation="MTGQTAYIESLSPWPKDGFGLERIRALLASLGDPQLRFPSIHVV
                     GTNGKSTVTRTIEALLAGAGLSVGSYVSPHVRGWSERIRVGGEEADFEAAIERVRAEA
                     ERLYAPQFEVLTAAALSAFAEAGVDVGVVEAGLGGRHDATNVLRSPVQVLTNVALEHT
                     EVLGTTREAIAAEKLAVVQPGAVVVLGEPEWEALARENGAGQVVVAGRSNLALGLAAA
                     EAFLGRPVDGHVDVRLPGRLERLSEDPLEIWDGAHNLAALAYLLPRLPTARYIVVASI
                     LADKDVDGMLAALSVLGDRLVATSSSNERALSAADLALRAAPFFARVEPVEDPAGALT
                     HARATGEPVLVTGSLYLLADLAKDESVRWRTLATG"
     gene            4109..4630
                     /locus_tag="VF225_01705"
     CDS             4109..4630
                     /locus_tag="VF225_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7143998.1"
                     /translation="MIASTFSGIHDFFTSGTWQVILNLVYFFLIAFWLAVGYWVYKDA
                     KRRIEDPVLIATAVAIGLVIPYMGALVYMLFRPPEYLEDVRERELEIKAMEERLSRRD
                     LYCPVCRAEVSNEFLVCPVCTTKLKQACVNCKAPLEALWQVCPYCETPVEPSAVEFPT
                     FEVRPPRRRRRAE"
     gene            4643..5062
                     /gene="ndk"
                     /locus_tag="VF225_01710"
     CDS             4643..5062
                     /gene="ndk"
                     /locus_tag="VF225_01710"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7143999.1"
                     /translation="MAVERSLILIKPDAVERKLAGEILARIERRGFTLRAGKLIHVDR
                     KLGETHYAEHSEKPFFGELVEFITSGPTLALVVEGEGAIATLRKAIGATNPADAEPGS
                     IRGDLANSMPDNLVHGSDSPESAAREVALWFSDDELV"
BASE COUNT          851 a         1745 c         1786 g          844 t
ORIGIN      
        1 gtgggcgttc cacgtcttcc tgcggcacgt cgatcgagga ctgaacttgc aggagtcgat
       61 cgacgcgccc gagttccaca ccgatcacct gatctcgtcg ttctacccgc gcggattcgt
      121 gccgcgctcg ttggatctcg aggcgaggtt cgggtcggcg gtgatcgacg acctccgggc
      181 gcgcggacac gaggtcaccg tcacgccgga ctggtcgctc ggtcgggtca ctgccgtcca
      241 gaaggcggac ggccaactgc gcgccgcggc gaatccccgc gggatgcagg gctacgcggc
      301 cggccggtaa ccaccagcgt ccctgagtcg ccttcaagcg aggctaggat cgcgcgcgag
      361 atggaatctc tctacgagcc cgagggtgtc gaggagcgct ggcagcgcac ctgggagacg
      421 gagggtcact acaacgccga cccggacccg tcgcgggaga gcttcgtgat cgcgcacccg
      481 ccgccgaacg tgacgggcga cctccacatg ggccacgcgc tgcagctttc cctggccgac
      541 acgatcatcc gcacgcgtcg catgcagggc tacaacgccc ttttccagcc gggcttcgac
      601 catgccggga tctcgacgca gaacgcggtc gagaagcacc tcgcgcagga gggcaagagc
      661 cgccaggatc tcggccgcga agccttcgta gccctcgtct gggagtggct gcgcgagtac
      721 ggcggcaaga tcatgctcca gttccggcgc atcggcgctt cgatggacta ccggcgcgag
      781 cgcttcacga tggacgacgc ttacgtgcac gcggtgatgc gcttcttcgt gcatctctat
      841 ggcaagggtt ggatctaccg cgcgaacaga atcatcaact ggtgcccgtt ccaccagacg
      901 gcgctctccg acctcgaggt cgcacacgtc gagctggacg atgccctgac ctatgcgcgt
      961 tacccgctcg ctgacggcgg gggccacatc acgattgcaa ccgtgaggcc ggcgacgatc
     1021 ctggccgacg tggcagtggc cgtgcatcct gaagacgatc gctatcggaa cctggtcggt
     1081 cgcgaggtga tcgtcccagt ggtcgggcgc cgcgtgccgg tgatcgccga cgaacgcgtt
     1141 gagcgtgagt tcggcaccgg cgcgctcaag gtcacgccgg gccacgaccc gctcgactac
     1201 gagatcggcc gcgaccacgg cctgcccgag ctcaccgtga tcggaccgga cggacgaatg
     1261 aacgaggagg ccggcgagct cgcggggttg acgcaggagg aagccgacca gcgcgtactc
     1321 gactggctga aggagcacgg tcagctcgag aagcgggagt cccaccgtca caccgttgcg
     1381 ctctgcgatc gctgccacag ccgcatcgag ccgcgcatct cgctccagtg gtggtgctcg
     1441 atggaggagc tgcgacagcc ggcgctcgag gcgctccgct ccggccgtgt ccgctaccac
     1501 ccggagtcgc agcaccgttt tgcgatcaag tcactcgagg acgctccgga ctggaacatc
     1561 tcccgccaga tctggtgggg ccatcagctg ccggtgtggg agtgtccgga cggacacatc
     1621 acggtcgcgg agacagagcc cgacgcgtgc gcacagtgcg ggtcgcacga gctgacgcgc
     1681 tccaccgacg tcctcgacac gtggttctca tccgcgctgt ggcccttcgc gacactcggc
     1741 tggccggacg acaccgagga tctgcgcacc ttctacccgg gtgatctgaa cacgacggcg
     1801 cgggacatca tccggctctg ggagaaccgg atgatcttca gcgggctgga gatgatgggg
     1861 gacgtcccct tccacgacgt cgtcatccac tcgaccgtgc atgcgccgac cggcggccgg
     1921 atgtcgaaga gccttggcac aggaatgaat ccgcttgcgg ccatcgagac ctacggtgcg
     1981 gacgcgacgc gctacgggct gatgaagatg gcgtcctcgc aggacgtccg cttctcggag
     2041 ggcgcgatcg aggagggccg gaagctcgcg aacaagctct ggaacgtggc gcggctgatc
     2101 cttcaggctt cggagggggc tgcgccggaa gagcgtccgc gcacggtcga ggagcgctgg
     2161 atcctcgccc gcctgtcgca gacacaacgc gagctggagc gactgctggg ggagttcgac
     2221 ttctcccacc tggtgcacga gctctatcac ctcaccttcg acgatttctg cgactggtac
     2281 gccgaggccg tgaaggggcg gttgtacgag gccgacgagg atgcgcgcgc aactgcagtc
     2341 gctgcgctcg agcggttgct gaagctcctg catccggcgc tgcctcacgt gaccgaagag
     2401 atctggtcga acttgcccgc gcgtcaaacg cggctgatcg tcgcgccctg gccggtggcg
     2461 ggcagcgagg cggacgcagg cgcgctcact gcggtgcagg aagccgcggt catgttccgg
     2521 cgcagcggcg tgctgccgaa gctcgaaggc gaggaacggc ggatcttcga cgcggtcgtg
     2581 aaacccggcg agggagcagg cgacggcaac gcggccgcgg agatcgagcg gttgcacaaa
     2641 gagatcgcgc gcgccgaagg aatgctcgcg aacgaccgct tcacacagag cgcgcctgcg
     2701 gaggtcgtcg aggccgagcg cgagaagctc gcgcgttacc ggcgggaact cgatgccatc
     2761 agcagttagc ggcttgaccg gccacgcctg gcggcccctg gcccgcgaga acgctgcgcc
     2821 acggggcgtc ctcagtcggg cccatgcagt catgcatggg ccctgcctgc gtccttccgt
     2881 agcttggttc tcgcgaccca ggaatccacc aacgtgaccg gccaaactgc atacatcgag
     2941 tctctgagcc cctggccgaa ggacggcttc gggctcgagc ggatccgggc gctcctcgcg
     3001 agcctcgggg acccccagct tcggtttccg tcgattcacg tggtcgggac gaacggcaag
     3061 tcgaccgtga ctcgcacgat cgaagcactg cttgcgggcg ccgggttgtc ggtcggctct
     3121 tacgtttcgc cgcacgtgcg cgggtggagc gagcggatcc gcgtgggcgg ggaggaggcc
     3181 gacttcgagg cggcgatcga gcgcgtgcgc gccgaagcgg agcgtctgta cgcgccgcag
     3241 ttcgaggtgc tcaccgcagc agccttgagc gcgtttgcag aagcgggcgt ggacgtgggc
     3301 gtggtcgagg ccgggctcgg cgggcgacac gatgcgacga acgtccttcg ttcgccggtg
     3361 caggtcttga cgaacgtcgc gctcgagcac accgaggtgc tcggcacgac gcgtgaggcg
     3421 atcgccgccg agaagctcgc ggtcgtgcag cccggcgcgg tcgtcgtgct cggggagccc
     3481 gagtgggagg cgcttgcgcg cgagaacgga gccggacagg tcgtcgtcgc cggccgcagc
     3541 aacctcgccc tcggcctcgc ggccgcggag gccttcctcg gtcgccctgt ggacggtcac
     3601 gtcgatgtca ggctcccggg tcgcctcgag cggctcagcg aagatcccct cgagatctgg
     3661 gacggcgcgc acaatctcgc cgcgctcgcc tatctcctgc cgcggctgcc gaccgcccgc
     3721 tacatcgtcg tcgcctcgat cctcgcggac aaggacgtcg acgggatgct ggctgccctc
     3781 tccgtcctcg gggaccggct ggtggccacc tcttcgagca acgagcgcgc cctatccgcc
     3841 gcggatctgg ccctgcgagc tgcacccttc ttcgctcggg tcgagccggt cgaggacccg
     3901 gccggggcgc tcacacacgc ccgcgccacg ggagaacccg ttctggtgac cgggtccctt
     3961 tacctcctgg ccgatctcgc caaggatgag agcgtacgat ggcgaacgtt ggcgacaggc
     4021 tgatcgtctt tgccttcgcc gccttcgtct tggcggcgat cgtcgggctc gcgtttcttg
     4081 tcgggtacct aataggaaga atccttctgt gatcgccagc accttcagcg gcatccacga
     4141 cttcttcacc tccggcacgt ggcaggtgat cctgaacctg gtctatttct tcctgatcgc
     4201 gttctggctc gccgtcggct actgggtcta caaggacgcc aagcgccgga tcgaggaccc
     4261 ggtcctgatc gcaaccgccg tcgcgatcgg tctcgtcatc ccctacatgg gcgcgctggt
     4321 ctacatgctc ttccgcccgc cggagtatct cgaggacgtg cgcgagcgag agctcgagat
     4381 caaggcgatg gaggagcggc tctcgaggcg cgacctctac tgcccggtgt gccgcgccga
     4441 ggtctcgaac gagttcctcg tctgcccggt ctgcacaacg aagctgaagc aggcctgcgt
     4501 caactgcaag gcgccgctag aggcgctctg gcaagtctgc ccgtactgcg agacaccggt
     4561 cgagccgtcg gcggtcgagt tcccgacctt cgaggtccga ccgcccaggc gcaggcggcg
     4621 cgccgagtaa actcgtccgc ccatggcggt cgagcgttca ctcatcctga tcaagccgga
     4681 cgctgtcgag cggaaactcg ccggggagat cttggcgcgc atcgaacgcc gcggcttcac
     4741 gctccgcgcc gggaagctca ttcacgtcga tcgcaagctc ggagagactc actacgcgga
     4801 gcacagcgag aagccgttct tcggcgagct cgtcgagttc atcacgtccg ggcccacgct
     4861 tgccctggtc gtcgagggtg aaggcgcgat cgcgacgctg cgcaaggcga ttggcgcgac
     4921 gaaccccgcc gacgcggagc ccggctcgat tcgcggcgat ctcgcgaact cgatgcccga
     4981 caacctcgtg cacggctcgg actcgccgga atcggctgca cgcgaggtcg cgctctggtt
     5041 ctcggacgat gagctcgtct gagcgacccg actacgttca gcagaacgtc gcgaactgga
     5101 ctcgcgcgaa tacggactac accgacgcct ccgcggcgcg ggcctgggcg caggacgaga
     5161 tcacgtgggg cgtgttcgaa gcgcccgagt cgaagctgaa cctgctcggg gacgtcaacg
     5221 gcctcg
//
LOCUS       DASVEU010000044         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1196544,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000044 DASVEU010000000
VERSION     DASVEU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196544"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VF225_01715"
     CDS             complement(<1..724)
                     /locus_tag="VF225_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144000.1"
                     /translation="MSRAPTMTFRKPVKCPRTGLWLARYKTPSGSIRQAGRFKAKSDA
                     AAAIETATKKAWGASVETRRELTLREFFELWPERFPRPARTESTNLHRVSRYILPYLP
                     EGGNFAFEHLRRPMLRDIQRQLLAEGLAKRTIDHAFSALSTMLEDAIEDDQTDSNPAK
                     GFRVKVNDPRLRPARAPRSRRSVPPAEIAAFMAHVPRPHHAICLTPLLTGARTQEIFT
                     MRRGEIDRELELIYLHETADRYG"
     gene            complement(721..963)
                     /locus_tag="VF225_01720"
     CDS             complement(721..963)
                     /locus_tag="VF225_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX7144001.1"
                     /translation="MSANIHPHPRARVHDVELERVSIRPLLTKTSARVYLAVSERTLD
                     RLVHDGELAAYRVRGQLRFRFDDIEAYLSARRVRVA"
     gene            1174..1386
                     /locus_tag="VF225_01725"
     CDS             1174..1386
                     /locus_tag="VF225_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144002.1"
                     /translation="MSTPSSNARWQGRQYVLQDGEIVLEADEVRPEDAAFVLLDEHTV
                     EPITSAQEANQSRLILALIFRLLAIA"
     gene            complement(1432..2028)
                     /locus_tag="VF225_01730"
     CDS             complement(1432..2028)
                     /locus_tag="VF225_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7144003.1"
                     /translation="MTRRDEQKEENRAKLVAAARKVFAEKGFGETTARAIVAETDLAV
                     GTFYNYFSDKEAAFRAVLDEFSRHARAAAHERRAAEGASLEERVYGAYRAYFELAAAN
                     PELFVFRSRNAETVARLGGESVDDAAVSELVEDMRQWVEESQLPHAALEWLPEIARLI
                     AGGGLNLAANLHAEGARDPDSLARLCTTLLLDGLRGLK"
BASE COUNT          374 a          784 c          796 g          383 t
ORIGIN      
        1 ggccgtagcg atcagccgtc tcgtggaggt agatcagctc gagttcgcga tcgatctccc
       61 cgcggcgcat cgtgaagatc tcctgggtgc gcgcgcccgt caggagtggc gtcaggcaga
      121 tcgcgtggtg cggtcgcggc acgtgcgcca tgaaggccgc gatctccgcc ggcgggaccg
      181 accggcgcga cctcggcgcg cgcgccggcc ggagccgcgg atcgttgacc ttgacccgga
      241 accctttggc cgggttggaa tccgtctggt cgtcctcgat cgcgtcctcg agcatcgtcg
      301 acagagcgga gaacgcgtga tcgatcgtgc gcttggcgag tccctcggcg agcagctggc
      361 gctggatgtc gcgaagcatc ggacggcgca ggtgttcgaa ggcgaagttg ccgccctcgg
      421 ggaggtacgg gaggatgtag cggctcacgc ggtgcaggtt cgtggactcg gtgcgcgcgg
      481 ggcgcgggaa gcgctcgggc cacagctcga agaactcgcg gagtgtgagc tcgcgccgcg
      541 tctcgacgct cgcaccccac gccttcttgg tcgcggtctc gatcgcggcg gcggcgtccg
      601 acttggcctt gaagcgtccc gcctggcgaa tgctgccgct aggcgtcttg tagcgcgcaa
      661 gccagagtcc agtgcggggg cacttgaccg gcttgcggaa ggtcatcgtc ggcgcacggc
      721 tcacgcaaca cgcactcgcc ttgcgctcag gtacgcctcg atgtcgtcga agcgaaaccg
      781 cagctgaccg cgaacgcgat aggcggcgag ttcgccgtcg tggacgagcc gatcgagcgt
      841 acgctcggac actgccaggt acacgcgggc gctcgtcttc gtcaggagcg gccggattga
      901 gactcgctcg agctcgacat catgtacacg cgcgcgcggg tgcgggtgaa tattggcgct
      961 catgcgagcc ccctcacgcg cccatgcacg aaccgaacct ggatcgtcat gaacgaatga
     1021 aaccggcgca ggagacggaa attggagcct gtgcagcaga ttcatgccgc caccgcgcct
     1081 cagctcacgc gacacaccag gtccatcagc gacacgtggg tcagtgcgtg ggcgagttcg
     1141 cgccggtagc gcggctcgag ctgcgtcaac agcgtgagca cgccgtcgtc gaacgcgcgg
     1201 tggcagggcc ggcagtacgt gctgcaggac ggcgagatcg tgctggaagc tgacgaagtt
     1261 cgacccgagg acgccgcgtt cgtgctgctc gacgaacaca cagtggagcc gatcacctcg
     1321 gcgcaggaag cgaaccagag ccggctcatc ctggcgctca tcttcaggct tctcgcaatc
     1381 gcctgatcca gactcgatgg acgcaatgac gcacggcacc tcgccggccg tctacttcag
     1441 tccacggagg ccgtcgagga gcagcgtggt gcacaaacgg gcgagggagt cggggtcgcg
     1501 ggccccctcg gcgtggaggt tcgccgcgag attaagccca ccaccagcga tcaggcgcgc
     1561 gatctccggt agccactcaa gcgccgcatg tggaagctgc gattcctcga cccactggcg
     1621 catgtcctcg accagctcgc tgacggctgc gtcgtcgacg ctctcgccgc ccaaccgcgc
     1681 aaccgtctcg gcgttgcgag agcggaacac gaacagttcc gggttcgcgg cggccagctc
     1741 gaagtaggcg cgataggcgc cgtagacacg ctcctcgagc gacgctccct ccgcggcacg
     1801 ccgctcatgc gcggcggctc gcgcgtggcg tgagaattca tcgagcacag cgcggaacgc
     1861 cgcttcctta tcgctgaagt agttgtagaa ggttccgact gctagatcgg tctccgccac
     1921 gatcgctcgc gcggtcgtct caccgaaccc tttctcggcg aacaccttgc gcgcggcagc
     1981 gacgagcttc gcgcggtttt cttccttctg ctcgtcccgc cgggtcattc tcagcggggc
     2041 cggcttatac agtcagctct gattgtttgt gtgggagcgg gagccgcagc gcctgacctt
     2101 gcgagacagc ttgatccgcg tcccgtcgtc gagcctgacg gcggtgacca ccctgccccg
     2161 tactcgccgc gttcgagcgc ctgcgacccg gcgggcgtcc cgcccgcgga cggagaccct
     2221 gactctgccg cccgagcacc gctggacctg cctgagccgc agcccgcacg ccgggaccgg
     2281 gtccccgacg ttcgagttcc cctcgatgag ggtgcggcag ccgagctcgc tcgcgta
//
LOCUS       DASVEU010000045         6158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_808294,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000045 DASVEU010000000
VERSION     DASVEU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808294"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VF225_01735"
     CDS             <1..412
                     /locus_tag="VF225_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144004.1"
                     /translation="DATTAAKSPAGRAGDIAERSVLVPVGAALIARDRVRTSVEELRA
                     TYSTRKKTENELRRFERRGTSALKAVERELRDRRTRLEKEVRAVIKDIETRTEPVVKN
                     VELVGARVENAVQGGMTAATKASATVQERLAALA"
     gene            complement(488..1546)
                     /locus_tag="VF225_01740"
     CDS             complement(488..1546)
                     /locus_tag="VF225_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7144005.1"
                     /translation="MSVPMTPPASASRYELEQVWLSASERLLDWDDAFHELLLGDRIR
                     MRAFRAAIDEVVQPGMTVLDLGTGTGILAEWALKNGAARVYGLELNEDILAAAVERLG
                     TAGYGASFEPIHGMSLDVTLPEAVDVIVSETLGNLADNEGTVEILDDARRRFLAPGGL
                     MIPSHVESYLLPVAAERAHTLVTTCTVADVDPGDWLSRVKEHGGRDQFDVYYDALLPR
                     SSHLAPARVARRYDLGRANDATYCETLTWTVHEAGPLTGFKGYFIATLSPTVALDISG
                     DDPGSGARDTRTSSDSWKHCYLPIANPVLVRRDDRIRLQLTRAARPDRPFQQAYHWNG
                     AVLRGRVPIATFSNRQHR"
     gene            complement(1791..2624)
                     /locus_tag="VF225_01745"
     CDS             complement(1791..2624)
                     /locus_tag="VF225_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX7144006.1"
                     /translation="MSATRATQASLQRLFELNRSVPSAARYREIEAIEAVWLEPILAQ
                     QEALTPQLSSAPEIAPLIEESIADGDSDGRSSADFLANDATREQFRAVVADYAVDGLT
                     EAQNFFAAIPRLPIRAQMAVMRVLIDEFGCGNLQRAHSELYMDLLRELDLPTDLASHL
                     PDVSAETLAFVNCFYWLASRAPDVEYFLGALAYLEAYIPTGFSCFDVACRRLGIKHSL
                     YYSEHIHIDEFHKQELQIAIREYDRAEGCDYAKLWVGMTLLRRLLGDSFDAAIDRARA
                     L"
     gene            complement(2621..3409)
                     /locus_tag="VF225_01750"
     CDS             complement(2621..3409)
                     /locus_tag="VF225_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7144007.1"
                     /translation="MSLRGVLVPLVTPLTESGEVSTTCVGALIESVRPYAGGLLPGLS
                     TGEGWRLSKQQWTDMLAATVEHARDLPVIAGVLLADAAKIAERLLGVSERLHAVAVAP
                     PFYRAAKQDDIYAHFSVLRSATTTPIVIYNESHLSGANMNVKTIRRVCELGRIAAIKD
                     SSGRIDVGRELAATTGVPVFQGSEHLLSNSTPLAGSVVGLANLEPGLCAAAQREASAA
                     TAGAVAHAVRRYGLDGPDWYANVKRELVRRNVIRASAIVGEDRR"
     gene            complement(3406..3897)
                     /locus_tag="VF225_01755"
     CDS             complement(3406..3897)
                     /locus_tag="VF225_01755"
                     /inference="COORDINATES: protein motif:HMM:NF039371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6190 family protein"
                     /protein_id="HEX7144008.1"
                     /translation="MSSSPLIDASVFMGMHAQDDVTRLACMSFMATRFDAGVVFSWEQ
                     IGRCDDIVWSYARDVQDAYYPFMDALHSTMSFTRRSYDAADLELALRDRRLAGLPMHE
                     SLAVAMASNRNASLVTISDRLHERRGLPVRRPAGTPAASFPEPVATLYARSLRLRVDG
                     GEL"
     gene            complement(3890..5083)
                     /locus_tag="VF225_01760"
     CDS             complement(3890..5083)
                     /locus_tag="VF225_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7144009.1"
                     /translation="MSLNVLSSVRAPAVAPVAADLTQHEILGLTKAFNLSDAHTHQRQ
                     SYTQRRIVERLPELWYRAERSPQSESERAFVDAFCRLHNQPTALAKNRALLSYAASVS
                     TLIAGMYLRKRRLSVTLIEPCFDNLHDVLHNAGVPVVPIDEHCFEDVTRIYSNLAHHV
                     RTDALFLVDPNNPTGFSLLQHGREGFEEVIRYCSDHDKILLLDFCFAAFALADPAIER
                     FDVYSLLEASGVTYIAIEDTGKAWPVQDAKCAILMASDDIWSDIYNIHTSVLLNVSPF
                     VLQLLQAYIEDSIEDDLASVRHVSLANREAATSALAGSILEHQTPTVDVSVAWFKINH
                     DTLWATRLHADLLDIGVAVLPGTNFFWSTPRRGQDYVRIALARDPQMFASAVTRLSDA
                     LRFYE"
     gene            complement(5161..6090)
                     /locus_tag="VF225_01765"
     CDS             complement(5161..6090)
                     /locus_tag="VF225_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7144010.1"
                     /translation="MTPDLRQLRYFVAVAQELNFTRAAERLHLAQPRLSAAIRQLEAQ
                     LGVTLFKRTTRQVELTLSGKLLLEKGHTLLSHADEVFSAVRDVERTPVGRLDVGVAPT
                     ARFGLAAKLFGACATEAAGVMLYPREDTTGVLLRDLHAGRLDLVVGFCAQPDDTLERE
                     RLRDEPAVLHVDASHPLASRDSVALEELRDESVIVAGGPDSPGYTATVVELCRAAGFE
                     PNTVPDPYPDLGLQAIREGLGVVIYVRTAFAPDLGGSAFLPIAPPVTMPFDLFWRRGI
                     NSGALDAALGVARKLRDREDWLATPPARTSHDR"
BASE COUNT          978 a         1946 c         2244 g          990 t
ORIGIN      
        1 cgacgcgacg accgccgcca agagccccgc cggacgcgcc ggcgacatcg ccgagcggtc
       61 ggtgctcgtg ccggtcgggg ccgcgctcat cgcgcgcgac cgcgtcagaa cctcggtcga
      121 ggagctgcgc gcgacctact cgacgcgcaa gaagaccgag aacgagctgc ggcgcttcga
      181 gcgacgcggc acgagcgcgc tgaaggcggt cgagcgcgag ctgcgcgacc gccgcacgcg
      241 cctggagaag gaggtccgcg cggtcatcaa ggacatcgag acccgcaccg agccggtcgt
      301 caagaacgtg gagctcgtcg gcgctcgcgt ggagaacgcc gtccagggtg gcatgacggc
      361 cgccaccaag gcatcggcga cggtgcagga gcggctcgcc gcgctggcct gagacggcga
      421 cccgcggccc ggccggcgcc ggtccggccg cggggggcgt gtctgccacg ccttgacccg
      481 taccccgcta acgatgttgc cggttgctga acgtggcgat cggaacccga ccgcgcagca
      541 ccgcaccatt ccagtggtac gcctgctgga acggccggtc cgggcgtgca gcacgcgtga
      601 gctgcaggcg gatgcggtcg tcgcgacgga cgaggaccgg gttcgcgatc gggaggtagc
      661 agtgcttcca gctatcggag cttgtgcggg tgtcgcgcgc gccggacccc ggatcgtctc
      721 cggatatgtc cagcgcgacc gtgggcgaca gcgtggcgat gaagtagccc ttgaaaccgg
      781 tgagcgggcc cgcctcgtga accgtccacg tcagggtctc gcagtacgtc gcgtcgttcg
      841 cgcggccgag gtcatagcgg cgcgcgaccc gcgcgggggc aaggtggctc gaccttggga
      901 gcaatgcgtc gtagtagacg tcgaactgat cgcggccacc gtgctccttc acccgcgaga
      961 gccagtcccc ggggtcgacg tccgcaacgg tgcatgtggt cacaagcgtg tgtgctcgct
     1021 cggcggcgac ggggaggagg tagctctcta cgtgcgatgg gatcatcagt ccgccaggcg
     1081 cgaggaaccg gcggcgtgcg tcgtcgagga tctcgaccgt cccttcgttg tcggcgaggt
     1141 tgccgagcgt ctcggagacg atgacgtcga cggcttcggg tagcgtcacg tccagggaca
     1201 tgccgtggat gggctcgaag gaggcgccgt agcccgccgt gcccaggcgc tcaacggctg
     1261 ccgcgaggat gtcctcgttc aactcgaggc cgtagacgcg ggcagccccg ttcttcagcg
     1321 cccattcagc gaggattccg gtgcccgtgc cgaggtcgag cacggtcatg cccggctgta
     1381 cgacctcgtc gatcgcggcc cggaacgctc gcatcctgat ccgatcgccc agcagcagct
     1441 cgtggaaggc gtcgtcccag tccaggagac gctcggaggc ggagagccag acctgctcga
     1501 gctcgtatcg ggaagcgctg gcaggcggcg tcatgggcac tgacacggaa gctcctcggt
     1561 gtccagcgcg tcgttgcttg ggccggtgac cactgcgccc gtcgcgaggt cgaagctcga
     1621 gcgatgcagt gggcaggtga tgcggagcct cgccgagtcg acatatccgt agagcagcag
     1681 gccgtcccgg tgcgggcacc tggcggctgc caggaacgcg cggccagctg cctgaacgcg
     1741 gacctttccc tcgccggccg gctcgacgga cgcggctgta tcgcgactcg tcacagcgcc
     1801 cgcgctctgt cgatggccgc gtcgaacgag tctccgagta gccgccgcag cagcgtcatt
     1861 ccgacccaca gcttggcgta gtcgcagccc tcggctctgt cgtactcgcg gatggcgatc
     1921 tggagctcct gcttgtggaa ctcgtcgatg tggatgtgct cgctgtagta caggctgtgc
     1981 ttgatcccga gccggcgaca cgccacgtcg aagcaggaga atcccgtcgg gatgtatgcc
     2041 tcgaggtacg ccagcgctcc gaggaagtac tcgacgtcgg gggctcgact cgccagccag
     2101 tagaagcagt tgacgaacgc cagggtttcg gcgctcacgt cgggaagatg gcttgcgagg
     2161 tccgtgggga ggtcgagctc gcgcagcagg tccatgtaca gctcgctgtg ggcacgctgc
     2221 aggttgccgc agccgaactc gtcgatgagc acgcgcatca cggccatctg tgccctgatg
     2281 ggcagccgcg ggatggccgc gaagaagttc tgcgcctcgg tcagcccgtc gacggcgtag
     2341 tcggccacca ccgcgcggaa ctgctcgcgc gtcgcgtcat tggcgaggaa gtcggccgag
     2401 gagcgcccgt cgctgtcgcc gtccgcgatg gactcctcga tgagtggcgc gatctctggc
     2461 gctgacgaga gctgtggcgt gagcgcttcc tgctgggcga ggatcggctc gagccagacg
     2521 gcttcgatcg cttcgatctc cctgtagcgg gccgcgctcg ggacggagcg gttcagctcg
     2581 aagaggcgct gaagacttgc ctgcgtggcg cgtgttgcgc tcatcgacgg tcctcaccca
     2641 cgatcgcgct cgcacggatg acgtttcgtc gcaccagctc gcgcttgacg tttgcgtacc
     2701 agtcaggacc gtcgagcccg tagcggcgta ctgcgtgcgc cacggcgccg gcggtcgccg
     2761 cggaggcctc ccgttgcgcc gcggcacaga gcccgggctc gaggttggcc aagccgacga
     2821 cggagccggc gaggggcgtg gagttgctca gcaggtgctc cgagccttgg aacaccggca
     2881 cgccggtcgt cgctgcgagc tcgcggccga cgtcgatgcg gccgctcgag tccttgatcg
     2941 cggcgatgcg gccgagctcg cagaccctgc gaatggtctt gacgttcatg ttcgctcccg
     3001 agaggtgcga ttcgttgtag atgacgatcg gcgtcgtcgt ggccgatcgg agcacgctga
     3061 agtgcgcgta gatgtcgtcc tgcttcgcgg cgcgataaaa cggcggcgcc acggcgacgg
     3121 cgtgcaggcg ctcgctgacc cccaggaggc gttcggcgat cttcgcggca tcggcgagca
     3181 gcacgccggc gatgacgggc agatcgcgcg cgtgctcgac ggtggccgcg agcatgtcgg
     3241 tccattgctg cttcgacagg cgccacccct cgccggtgct caaccccggc agcagcccgc
     3301 cggcgtaggg ccgcacgctc tcgatcaagg cgccgacgca tgtggtcgag acctcaccgc
     3361 tctcggtcag gggcgtcacg agcgggacga gcacgccgcg caggctcaca gctcgcctcc
     3421 gtcgacgcgc agtcgcaagg accgggcgta gagcgtggcg accggttcgg ggaagctcgc
     3481 ggcaggcgtg ccggctgggc gtcgcaccgg cagaccgcgg cgttcgtgca ggcgatcgct
     3541 gatcgtgacg agggaggcgt tgcgattcga ggccatcgcg acggcgagcg actcgtgcat
     3601 cggcaggccg gcgagcctgc gatctcgcag ggccagctcg aggtcggcgg cgtcatacga
     3661 tcgccgggtg aacgacatcg tgctgtgcag ggcgtccatg aacgggtagt aggcgtcctg
     3721 gacgtcgcgg gcgtaggacc agacgatgtc atcgcagcgg ccgatctgct cccagctgaa
     3781 cacgacgcct gcgtcgaacc gggtcgccat gaacgacatg cacgcaagac gcgtgacgtc
     3841 gtcttgcgcg tgcatcccca tgaagaccga cgcgtcgatc agcgggctgc tactcataga
     3901 agcgcagcgc gtcactgagc cgggtcacgg cggaggcgaa catctgcggg tcgcgggcga
     3961 gggcgatgcg gacgtagtcc tgcccgcgcc tgggcgtgct ccagaagaag ttggtgcctg
     4021 gtagaacggc gacgccgatg tccaagaggt cggcgtggag cctcgtggcc cagagcgtgt
     4081 cgtggttgat cttgaaccac gccacgctta cgtcgaccgt cggcgtttga tgttcgagga
     4141 tcgagccggc gagggcgctc gtcgcagcct cacggttggc cagggagacg tggcgcaccg
     4201 acgcgaggtc gtcttcgatc gagtcctcga tgtacgcctg cagcagctgg agcacgaagg
     4261 gcgacacgtt gaggagcacg ctcgtgtgga tgttgtagat gtcgctccag atgtcgtcgc
     4321 tggccatgag gatcgcgcac ttggcgtcct gcaccggcca cgccttgccc gtgtcctcga
     4381 tggcgatgta ggtgaccccg gatgcctcca gcaggctgta gacgtcgaag cgctcgatcg
     4441 ccgggtcggc gagcgcgaac gcggcgaagc agaagtccaa gagcaggatc ttgtcgtgat
     4501 cgctgcagta gcggatgacc tcctcgaagc cttcccggcc gtgctgcagg aggctgaagc
     4561 cggttgggtt gttcgggtcc acgaggaaga gcgcgtcggt gcggacgtgg tgcgcgaggt
     4621 tcgagtagat cctcgtgacg tcctcgaagc agtgctcgtc gatcggcacg accgggacgc
     4681 cggcgttgtg gagcacgtcg tgcaggttgt cgaagcatgg ctcgatcaag gtgaccgaca
     4741 ggcgacgctt cctgaggtac atcccggcga tgagggtgga gaccgacgcg gcgtacgaga
     4801 gcagcgcccg gttcttcgcc aaggcggtcg gctggttgtg gagccggcag aacgcgtcca
     4861 caaaggcgcg ctcgctctcg ctctggggcg accgctcggc gcgataccac agttcgggaa
     4921 gcctttcgac gatgcgacgc tgcgtgtagg actggcgttg atgcgtgtgc gcgtccgaga
     4981 ggttgaacgc cttcgtcaga ccgaggatct catgttgcgt caggtctgcc gccacggggg
     5041 cgacagccgg agcgcgaaca ctgctgagta cgttgagcga cacaggttct cctgaaggtg
     5101 gggtcgtcgt aaagcctggc gaggcgtccg agcgtgatac caacctgcgg gaggtctttg
     5161 ttatcgatcg tgtgaagttc gtgcgggagg cgtggcgagc cagtcttcgc ggtcgcggag
     5221 cttgcgcgcg acgcccaatg cggcgtccag tgcgccgctg ttgatgccgc gccgccagaa
     5281 caggtcgaag ggcatcgtga ccgggggagc gatcggcagg aacgcggacc ccccgaggtc
     5341 gggggcgaag gcggttcgga cgtagatgac gacgccgaga ccttcgcgga tggcctgcaa
     5401 ccctagatcg ggatacgggt cggggacggt gttgggctcg aagcccgccg ctcggcaaag
     5461 ctccacgacg gtcgccgtgt atcccggcga gtcgggaccg ccggcgacga tgacgctctc
     5521 gtcgcgcagc tcttccagcg ccacgctgtc gcgggaggcc aacgggtggg acgcatcgac
     5581 atggaggacg gcgggctcgt cgcggagtcg ttcgcgctcg agggtgtcgt cgggttgagc
     5641 gcagaacccg acgacgagat cgagccggcc cgcatgcaga tcgcgcagca ggacgccggt
     5701 cgtgtcctcg cgcggataga gcatcacccc cgctgcctcg gtcgcacacg cgccgaagag
     5761 cttcgcggcc agtccgaacc gcgctgtcgg tgcgacgccg acgtccagcc ttccgaccgg
     5821 cgtccgctcg acgtcgcgga cggcggagaa gacctcgtcg gcatggctca gaagcgtgtg
     5881 acccttctcc aagagcagct ttcctgagag cgtcagctcg acttggcgcg tcgtgcgctt
     5941 gaacagcgtt acgccgagtt gcgcttccag ctggcggatt gctgcgctga gccgcggctg
     6001 agcgaggtgt aggcgctcgg cggcgcgcgt gaagttcagc tcctgtgcga cggcgacgaa
     6061 gtagcgcagc tgacgcaggt cgggggtcac gggagcactt catcacatga gcaggtcctg
     6121 acgggccgta tgacttcaga cccaaggcgt cttggaca
//
LOCUS       DASVEU010000046         4397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_422490,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000046 DASVEU010000000
VERSION     DASVEU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422490"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF225_01770"
     CDS             complement(<1..186)
                     /locus_tag="VF225_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144011.1"
                     /translation="MATERRTKARELPRQQGMSMKAIATEVGASLSSVSRWVRDVELT
                     EEQQTTLELQAFNGHVKG"
     gene            complement(228..298)
                     /locus_tag="VF225_01775"
     tRNA            complement(228..298)
                     /locus_tag="VF225_01775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(264..266),aa:Gly,seq:tcc)
     gene            322..1074
                     /locus_tag="VF225_01780"
     CDS             322..1074
                     /locus_tag="VF225_01780"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7144012.1"
                     /translation="MDPHSWSLHWDVLLAIGVLAAGYFWSQRRWPADARRRAAFDLAV
                     ILLLAVYVTPLHTIALHYLLSVHLLQNVVTAEWAPGLVVFALAPSLAHELERSTAVRV
                     LTHPLVALPLWLATYFVWHIPPIYDRALEQSGWLLHLEHLTYFFAGVLMWWPVAHGRY
                     SDGVKALYIFGAFVLAAPLGLLLALLPRPIYGFYKDAPQLWGLSDIRDQQIAGVTMAV
                     EQAIVFFAVFAYFFLRFLRTEHIAGVFSESSR"
     gene            complement(1055..1915)
                     /locus_tag="VF225_01785"
     CDS             complement(1055..1915)
                     /locus_tag="VF225_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7144013.1"
                     /translation="MVVLLAAGSALFFGALAVTIRFALVRGIDAEAAALVTTVIAGVI
                     CASLALVTGGFSGVAWRDTWPFFVTGLFAPGISQILFTRSVGVIGPSRTAIAVGISPV
                     LSAAIAVTVLGEPLHVALVLGTLFVVGGGTLLVSERGGPAAMLSLGVALSLGAALLFS
                     VRDNLVRWAARSSDVPGFVAATASLVSATLVILIVVASRPNARARIRLAARPFVPSGL
                     VYGISYACLYSAFDRGRVTIVAPLVATESLFAVLISIVLLRRSERIGPRLLLAAALVV
                     GGGALISSFR"
     gene            complement(1918..1992)
                     /locus_tag="VF225_01790"
     tRNA            complement(1918..1992)
                     /locus_tag="VF225_01790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1955..1957),aa:Pro,seq:tgg)
     gene            2025..2936
                     /locus_tag="VF225_01795"
     CDS             2025..2936
                     /locus_tag="VF225_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7144014.1"
                     /translation="MVQRRPGLGYAMVAVAATLFAVNGTVSKVILGSGISSGQLTAVR
                     CAGALIGLALIAAATRPRSLRVRTGDLPVLIALGIALAVVQWAYFFAIHRLDIGIALL
                     IQFVAPIFVALWARFVFHEPVRRRIWAALALSVAGLTLIVEIWHGGRLSGPGVAACGL
                     AAISLAAYFLIAEHGVRSRDPVSLSAWAFLFATIFWSLLAPWWTFPGRRVDDHVSLLG
                     NLASTHVHLWLLMLWMVVLGGVVPFGLIVSALRHISATRVGITAMLEPVVAIVVAWAW
                     LGESLRPVQLSGAALTLLGIGLAQTAR"
     gene            3014..3484
                     /locus_tag="VF225_01800"
     CDS             3014..3484
                     /locus_tag="VF225_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7144015.1"
                     /translation="MSIKNKILVAFLASFALLAPAAGAQARTLSSSEASLLQTMNSVR
                     MSSHLAPLRVDVRLLRAARAHSADMMQRHYFAHGGVAGRAVAQGARGPVFGEDLAWGT
                     GVGARWVVSAWLASPAHRAVLLRPGFRRVGIGIAFGAFAGHGGAAVFTADFAGR"
     gene            complement(3544..3981)
                     /locus_tag="VF225_01805"
     CDS             complement(3544..3981)
                     /locus_tag="VF225_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7144016.1"
                     /translation="MTKVLVIDDEAPIRLLCRVNLEAEGMDVIEAPDGPSGVDKARDE
                     RPDLILLDVMMPGLDGWRVAEQLFEDDRTLGIPIIFLTARAEFRDRARGLDIGGVDYV
                     TKPFNPLELAPLVQSLLDRLDRGERDELRAEKLSELRTLMESE"
     gene            complement(4035..>4397)
                     /locus_tag="VF225_01810"
     CDS             complement(4035..>4397)
                     /locus_tag="VF225_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7144017.1"
                     /translation="EPLAAEADPDKVRQVFNILVENALRYSPVGGTVTVGARRNHDRV
                     EVRIADEGIGIPAAERERIFRKFYRAESAARDGAAGTGLGLFIAKELVTAMGGRIWVD
                     STEGKGSSFSFELPVARE"
BASE COUNT          676 a         1503 c         1454 g          764 t
ORIGIN      
        1 tcccttcacg tgtccattga acgcttgtaa ctcgagcgtt gtctgctgtt cctcggtgag
       61 ctcgacgtct ctgacccaac ggctgactga agagagtgac gcgccgacct ctgtggcgat
      121 cgccttcatc gacatgccct gttgccgcgg cagctctcgt gccttagtcc gtcgttccgt
      181 tgccacgata gaaaacaggc tacagagagc gtcggacgcg tcgagcaagc gggcggcgag
      241 gatcgaactc gcgtacccag ggtggaaacc tggtgctttg ccactaagct acgcccgcgg
      301 tgtggcgagt agcgtagcgg cgtggatccg cattcgtggt cgctgcactg ggacgtgctg
      361 cttgcgatcg gcgtcctcgc tgcgggctac ttctggagcc agcgtcggtg gccggcggac
      421 gcgagacgca gggccgcgtt cgacctcgcg gtcatcctcc tcctcgccgt ctacgtgacc
      481 ccgctgcaca cgatcgcgct ccactatctg ctctcggttc acctcctgca gaacgtcgtc
      541 acggccgagt gggcgccggg gctcgtggtg ttcgcgctcg caccgtcgct cgcgcacgag
      601 ctcgagcgct cgacagccgt tcgcgtcctg acccatccgc tcgtcgcgct gccgctgtgg
      661 ctcgcgacct acttcgtctg gcacatcccg ccgatctacg accgcgctct cgagcagtcc
      721 ggctggctcc tgcacctcga gcatctgacg tacttcttcg caggtgtcct gatgtggtgg
      781 cccgtagcgc atgggcgcta ctccgacggc gtgaaggcgc tgtacatctt cggcgccttc
      841 gtgctcgccg ctccgctcgg gctgctgctc gccctcctgc cgcgtccgat ctacggcttc
      901 tacaaggacg cgccgcagct ctgggggcta tccgacatca gggatcagca gatcgcgggc
      961 gtgacgatgg cggtcgagca ggcaatcgtc ttcttcgcgg tgttcgcgta cttcttcctg
     1021 cgcttcctgc gcacggagca catcgcgggc gtgttcagcg aaagctcgag atgagcgcgc
     1081 cgccgccgac cacgagcgcg gctgcgagga gcaggcgcgg cccgatccgt tccgagcggc
     1141 gcagcagcac gatcgagatc agcaccgcga agagcgactc ggttgcgacc aggggcgcaa
     1201 cgatcgtgac gcgcccgcgg tcgaacgcgg agtagagaca cgcgtagctg atgccgtaga
     1261 cgagccccga cggcacgaac ggacgcgccg ccaggcggat acgcgcgcgc gcgtttggcc
     1321 gcgaagcgac cacgatcaag atcacgagcg tcgcggagac gagcgacgcg gtcgcggcaa
     1381 cgaagccggg cacgtcgctg ctgcgtgcag cccagcggac gaggttgtct cgaacggaga
     1441 acagcagggc cgcgccgaga ctgagggcga ctccgagcga gagcatcgcc gcaggaccac
     1501 cgcgctcact gacgagcagc gttccacctc cgacgacgaa cagcgtccct agtaccagcg
     1561 ccacgtgtag cggctcgccg aggacggtga ctgcaatggc ggccgacaga accggtgaga
     1621 tccccacggc gatcgccgtc cgtgacggcc cgatcacacc caccgagcgc gtgaacagaa
     1681 tctgactgat tcccggcgcg aacaagccgg tgacgaagaa cggccaggtg tccctccacg
     1741 cgacgccaga gaagcccccg gtcacaagtg cgagcgacgc acagatgacg ccggcgatga
     1801 cggtggtgac aagcgctgcg gcctcagcat cgatgccgcg gacaagcgcg aaacggatcg
     1861 tgacggcgag cgcgccgaag aacagcgccg agcccgcggc aaggaggacg accaaagtcg
     1921 gggcggcgag attcgaactc gcgacttctg cctcccaaag gcagcactct aaccaggctg
     1981 agctacgccc cgccgtgcgc agagtacatg tgtaggttcg cgcgatggtc cagcgacgcc
     2041 caggcctcgg ctacgcgatg gtcgccgttg ccgcgacact gttcgcggtc aacggcacgg
     2101 tctcgaaggt gatcctcggc tcggggatca gctccggcca gttgacggca gtgcgttgcg
     2161 ccggcgcgct gatcggactc gcgttgatcg ccgcggcgac gcggccgcga tcgctgcgag
     2221 ttcgaaccgg cgacctgcct gtgctgatcg cactcgggat cgccctcgcc gtcgttcaat
     2281 gggcctactt cttcgcgatc caccggctcg acatcggaat cgccctgctg atccagttcg
     2341 tcgcgccgat cttcgtcgcg ctctgggcac ggttcgtctt ccacgagcct gtccgccgcc
     2401 gcatctgggc ggcgctggcc ctctcggtcg ccgggctcac gctcatcgtc gagatctggc
     2461 acggcggacg cctcagcggt cccggggtgg ccgcctgtgg gctggcggcg atcagtctgg
     2521 ccgcgtactt cctgatcgcc gagcacggcg tgcgcagccg cgatccggtc tccctgtctg
     2581 cctgggcctt tctcttcgcg acgatcttct ggtctctgct cgcaccctgg tggaccttcc
     2641 ccggccggcg cgtcgacgac cacgtctcgc tgctggggaa tctggcatcg acgcacgtcc
     2701 acctctggct gctcatgctc tggatggtcg tcctgggcgg ggtcgtgccg ttcggactga
     2761 tcgtgagcgc cctccgccac atctcggcga ccagggtcgg aatcacggcg atgctggagc
     2821 ccgtcgtggc catcgtcgtc gcctgggcct ggctcggaga gtccctccgg ccggtccagc
     2881 tgtccggtgc ggctctcacc ctgctcggga tcggactcgc tcagacggcc cgttgaaaag
     2941 ggcgctctga gcgggccgga tctacccctt ttgggggagg cgacccccag acgaccgctc
     3001 cagactgccg cgaatgtcga ttaaaaacaa gattctcgta gcttttctgg caagtttcgc
     3061 cctgctggcg ccggcggccg gggctcaggc gcggacgctg agcagctcgg aagcctcgct
     3121 gctccagacg atgaactccg tccggatgag cagtcatctg gcccccctgc gagtggacgt
     3181 tcgtcttctg cgggctgccc gcgcccactc agcggacatg atgcagcggc attacttcgc
     3241 gcacggcggc gtcgcgggcc gcgccgtggc ccagggtgca cgtggccccg tcttcggcga
     3301 ggacctcgcc tggggaacgg gagtcggcgc tcggtgggtc gtcagtgcgt ggctggcgag
     3361 ccctgctcat cgagccgtcc tgctcaggcc gggcttccgc cgggtgggca tcggaatcgc
     3421 gttcggcgcg ttcgccggtc acggtggtgc cgcggtcttc acggccgatt tcgccggacg
     3481 ctaaagccgt cgggagcaag agagcgcggg gggccgtctc ggaatgacgg cctctcttgc
     3541 gctctattcg gactccatca gcgtccgcag ctcggagagc ttctcggcac gcagttcgtc
     3601 ccgctcgccg cggtcgagtc ggtcgagcag actctgtacg agcggcgcca gctcgagcgg
     3661 attgaacggc ttcgtcacgt agtcgacgcc gccgatgtcg agcccgcggg cacggtcgcg
     3721 aaattccgcc cgcgcggtga ggaagatgat cgggatgccc agggtccggt cgtcttcgaa
     3781 cagctgttcg gcgacccgcc acccgtcgag tccgggcatc atcacgtcga ggaggatcaa
     3841 atcgggtcgc tcgtcgcgcg ccttgtccac cccggagggg ccgtccggag cctcgatcac
     3901 gtccatgcct tctgcttcga ggttcactcg acagagcaga cgaatcggag cctcgtcgtc
     3961 gattacgaga actttggtca tgaggggacg caccaccggt gagtcgccgc cgctataccc
     4021 aaaccggctg ccatctattc ccgtgcaacg gggagctcga acgaaaagct cgagcctttc
     4081 ccttcggtgg agtcgaccca gatgcgaccg cccatcgcgg tgaccagttc cttggcgatg
     4141 aagagaccga gccccgtgcc ggccgcgccg tcccgcgcgg cggactcggc ccggtagaac
     4201 ttccggaaga ttcgctcgcg ctccgcggcc ggaatcccga ttccctcgtc ggcgatacgc
     4261 acctcgactc ggtcgtggtt gcgccgcgcc ccaaccgtga cggtgccgcc aaccggcgag
     4321 tagcggagcg cgttctcgac gaggatgttg aagacctgac gcaccttgtc gggatctgct
     4381 tcggccgcga gcggctc
//
LOCUS       DASVEU010000047         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_616276,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000047 DASVEU010000000
VERSION     DASVEU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616276"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="otsB"
                     /locus_tag="VF225_01815"
     CDS             <1..571
                     /gene="otsB"
                     /locus_tag="VF225_01815"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7144018.1"
                     /translation="LNERYALVACISGRDGADAQRVVGIPELVYVGNHGLELDDEAAE
                     WRERLQRFLADVGWPATENKGLTASLHYRGSEDEAAARAALEDVKTRAEHTGFVARFG
                     RKVLEVLPPVDVHKGTAVRHLLAERNLKRALYAGDDTTDLDGFRALDGLDVSVRVAVA
                     AEEGPRELREAAEIVVGDPLELMSLLKRL"
     gene            complement(593..1882)
                     /locus_tag="VF225_01820"
     CDS             complement(593..1882)
                     /locus_tag="VF225_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7144019.1"
                     /translation="MTFREDGAAALEWAARYLERVGDFPVLAQVEPGDIRSRLPAAPP
                     ERGEPFASVLRDLDEILLPGITHWQSPRFFAYFANSSAEPGILAELLAATLNSVAFIW
                     RTSPASTELEAHTLDWTAQLLGLPSGWHGHIEDTASISTIAALAAAREANGGHVVVCS
                     EEAHSSVERAARLLGLETRKVPLDSEFRLDPSAVDLTDAAALVATVGTTSTTAVDPVP
                     VLADACEAAGVWLHVDAAYAGSAWICPELRWSQAGVERADSLVVNAHKWLFTPMDCSL
                     LWTRRPEALRAAFSLVPEYLRTSDEAENLSDYGPALGRRFRSLKLWAVLRCYGREGLQ
                     ARIREAIRLAELFEGLVRAEPGWEVCAPRAFSVVCFRRSGSDDENEELLARVNETGEL
                     FISHTKLDGRYVLRLAIGNERTTEADVRRAWNVLQRG"
     gene            complement(1879..3570)
                     /gene="polX"
                     /locus_tag="VF225_01825"
     CDS             complement(1879..3570)
                     /gene="polX"
                     /locus_tag="VF225_01825"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX7144020.1"
                     /translation="MAGALPRNVDVAEQLELLADLLEIEGEASFRVLAYRRAATRIRE
                     TAGSVAELALAGRAKELQGIGKTIEEKIVQVVEDGEMHALTKRKKVIPAEVVSFMRLP
                     GLGPKTAARIWQELGVTTIDELKRAAEQEQLRTLAGLGAKTEERILKALAEKKQEPSD
                     RRLLGDGLGALLAVVEVLQEHPAAVKVSEAGSARRRKETFRDLDIIATAKDPETLIDY
                     FTKLKWVIEVVAKGPTKATVLSNEGLRFDLRVVPPESYGNLLQHFTGSKDHNVVLRER
                     AVKDGLSVSEYSITVVETGEELTFAEEEQVYERLGYQFIPPELRENSGELEAARKGEL
                     PQLVELGDLKGDLHTHTSYSDGRDSLEQMALAARSRGYAYYAVTDHPRGTLAEQDLEI
                     DALNERLKPFKILKGIEVNIRIDGSVSLPDEVLAERDWVMASLHAAFDRSPTERVLTA
                     MENPHVDCIGHLTARKINIRPPANVDVERVVAKALETGTFLEINAQPNRLDLRDTHAR
                     LAGEAGAKIAVNTDAHQLSALQHMEMGMAQARRAWLTKDQVLNTRTWSQIEKLRK"
     gene            3594..4307
                     /locus_tag="VF225_01830"
     CDS             3594..4307
                     /locus_tag="VF225_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX7144021.1"
                     /translation="MPKAVVCLPTYNERENIEPMLRALGDKGVRVLVIDDNSPDGTGE
                     LADRLAQELGYVDVLHRERKEGLGPAYLAGFRHALAGDAELVLEMDCDFSHDPDYVPR
                     LIAAVEDGADLALGSRYVPGGGVRNWGLLRRLISAGGSFYARIVLGVKVRDLTGGFKC
                     YRRAVLEAIDLDAVDSKGYAFQIETTYRALRAGFEVVEVPITFADREVGGSKMSKAIV
                     AEAIWKVPGLRLKALLGRI"
     gene            4425..4748
                     /gene="trxA"
                     /locus_tag="VF225_01835"
     CDS             4425..4748
                     /gene="trxA"
                     /locus_tag="VF225_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7144022.1"
                     /translation="MFEVTDDTFEQDVLGADTPVVLDFWAPWCGPCKAVEPILEQLES
                     DTRGRVEFAKLNIDENPLTAARYDVLSIPTAILFDGGEAKATVIGARPRSHYERVLAE
                     VLPAA"
     gene            complement(4722..>5118)
                     /locus_tag="VF225_01840"
     CDS             complement(4722..>5118)
                     /locus_tag="VF225_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7144023.1"
                     /translation="SGDQLVLTGPLGGAGAAFRRQSYVRPPVRLAEGRELAADAHALL
                     DVSDGLAVDAGHIASRSGCRVVVELERVPLASGAELQDLGFGEDYELLAAVSEPRRFT
                     AIGRCEEGAGVAITLNGEPYELGGWQHFR"
BASE COUNT          799 a         1752 c         1709 g          858 t
ORIGIN      
        1 cctgaacgag cgctacgcgc tcgtcgcctg tatttccggt cgtgacggtg cggatgcgca
       61 acgagtcgta ggcattcccg agctcgtcta cgtcggcaac cacgggctgg agctcgacga
      121 cgaggcggct gagtggcgcg agcggcttca acggttcctc gcggacgtcg gttggcccgc
      181 gacggagaac aagggcttga cggcatcgct ccactaccgc ggctcagagg acgaagccgc
      241 cgcgcgtgct gcgctcgagg acgtgaagac gcgtgctgaa cacacaggct tcgtcgcgcg
      301 cttcgggcgg aaggtgctcg aggtactgcc gccggtcgac gtgcacaagg gaaccgcggt
      361 caggcacctg cttgccgagc ggaacctcaa acgcgcgctc tacgccggcg acgacacgac
      421 cgacctcgac ggtttccgcg cgctcgacgg cctcgacgtg tccgtccggg tcgcagtggc
      481 agccgaggag ggcccacgag agcttcgcga ggcggccgag atcgtcgtcg gtgacccgct
      541 ggagctcatg tcgttgctaa agcggctcta gccgcgcact cgcaatccat tctcagccgc
      601 gctgcaagac gttccaggct cgccgcacgt cagcctcggt cgttcgctcg ttgccgatcg
      661 ctagtcgcag aacgtaccga ccgtcgagct tcgtgtgtga gatgaacaac tcgcccgttt
      721 cgttcacgcg cgcaagcagc tcttcgttct cgtcgtcgga gcccgacctg cggaaacaga
      781 ccacggagaa ggcgcgcgga gcacacacct cccagccggg ctcggcgcgc acgagaccct
      841 cgaacagctc cgccagcctg atcgcttccc tgatccgcgc ttgcagtccc tcgcggccgt
      901 agcagcgaag gactgcccac agcttgagcg agcggaagcg gcggccgagc gcggggccgt
      961 aatccgaaag gttctcggct tcgtcactcg tgcggaggta ttcaggcacg aggctgaagg
     1021 cggcgcggag cgcttcgggc ctgcgggtcc acagcaggga gcagtccatc ggcgtgaaca
     1081 gccacttgtg cgcgttgacg acgagcgagt ccgcgcgctc cacgcctgcc tgcgaccagc
     1141 gcagctcggg gcagatccag gcggagcccg cgtaggcggc gtcgacgtgg agccagacgc
     1201 ccgccgcctc gcatgcatct gcaagcacgg gcacgggatc gacggccgtc gtcgacgtcg
     1261 tcccgacggt agcgacgagc gctgccgcat cggtgagatc caccgctgac gggtcgaggc
     1321 ggaactcgga gtccaacggc accttgcgcg tctcgaggcc gagcaggcgc gcggcccgct
     1381 ccactgatga gtgcgcctcc tccgagcaca cgacaacgtg gccgccgttc gcctcacgcg
     1441 ccgcggcaag cgcggcgatc gtcgagatgg aagccgtgtc ctcgatgtgg ccgtgccagc
     1501 cggagggaag gccgaggagc tgtgctgtcc agtcgagcgt gtgcgcctcg agttcggtcg
     1561 acgcgggcga ggtgcgccag atgaatgcga ccgagttgag cgtggcagcg aggagctccg
     1621 cgaggatgcc cggctcggcc gagctgttgg cgaagtaggc gaagaagcgc gggctctgcc
     1681 agtgcgtgat gcccgggagg aggatctcgt cgaggtcgcg caacacggag gcgaacggct
     1741 cgcctcgctc cggtggcgcc gccggcagtc ggctccgaat gtcgcccggc tcgacctgcg
     1801 caagcacggg aaagtctccg acgcgctcga ggtaacgagc cgcccattcg agtgcagcgg
     1861 ctccgtcctc gcgaaaggtc actttcggag cttctcgatt tgcgaccagg tacgcgtgtt
     1921 caacacctga tcctttgtca gccacgcgcg ccgtgcctgg gccatgccca tctccatgtg
     1981 ctggagggcg ctcaactgat gtgcgtcggt gttgaccgcg atcttcgcgc cggcctcgcc
     2041 cgccagccgc gcgtgcgtat cccggaggtc gagccggttc ggctgcgcgt tgatctcgag
     2101 gaaggtcccc gtctccagcg ccttcgcgac gacgcgttcg acgtcgacat tcgcgggcgg
     2161 cctgatgttg atcttgcgcg ccgtgagatg accgatgcag tcgacgtgtg ggttctccat
     2221 cgccgtgagg acgcgttcgg tcggactgcg gtcgaaggca gcgtgcagcg aggccatcac
     2281 ccagtcccgc tccgcgagca cctcgtccgg caacgacacg gagccgtcga tgcggatgtt
     2341 gacctcgatg cccttgagga tcttgaacgg cttcagccgc tcgttgagcg cgtcgatctc
     2401 tagatcctgc tccgccagcg tcccgcgcgg atggtccgtg accgcgtagt acgcatagcc
     2461 cctagagcgg gccgccagtg ccatctgctc taacgagtca cgtccgtcgg aatagctcgt
     2521 gtgcgtgtgc agatcgccct tgaggtcacc gagctcgacg agctgcggca gctcgccctt
     2581 gcgcgctgcc tcgagctcgc ccgagttctc gcgcagctcg gggggaatga actggtagcc
     2641 gagtcgctcg tagacctgct cctcttcggc gaacgtcagc tcctcgccgg tctcgacgac
     2701 ggtgatcgag tactcggaaa cggacaagcc gtctttcacc gcgcgctccc gcagcacgac
     2761 attgtggtcc ttcgacccgg tgaaatgctg cagcaggttt ccgtacgact cgggcgggac
     2821 gacgcgcagg tcgaagcgca ggccctcgtt cgacaacacc gtggccttgg tcggtccttt
     2881 cgccacgact tcgatcaccc acttgagctt cgtgaagtag tcgatcagcg tctcgggatc
     2941 cttggccgtc gcgatgatgt cgaggtcgcg gaaggtctcc ttgcgccgtc gcgcacttcc
     3001 ggcctcggac accttcacgg ccgccgggtg ctcctgcagc acctcgacga cggcaagcag
     3061 agcccccagc ccgtccccca gcagccgccg gtcggatggc tcctgcttct tctcggcgag
     3121 cgccttgagg atccgctcct cggtcttcgc ccccaggccg gccagcgtgc gcagttgctc
     3181 ctgctctgct gcccgcttga gctcgtcgat cgtcgtgact ccgagctcct gccagatccg
     3241 agccgccgtc ttcgggccga gcccgggcag gcgcatgaag ctcaccacct ccgcgggaat
     3301 gaccttcttc cgcttcgtca gcgcatgcat ctcaccgtcc tcgacgacct ggacgatctt
     3361 ctcctcgatc gtcttgccga ttccctgcag ctccttggca cggccggcga gcgcgagctc
     3421 ggccaccgag ccggccgtct cgcggatccg ggtggcggcg cgacgatagg cgaggacgcg
     3481 gaaggaagcc tctccttcga tctcgagcag gtcggcgagg agctccagtt gctcggccac
     3541 gtccacattg cgaggcagag ctccggccac acgttgaata tacgtaggtg caggtgccca
     3601 aagccgtcgt ctgcctcccc acctacaacg agcgcgagaa catcgagccg atgctgcgcg
     3661 cgcttggtga caagggcgtc cgcgttctgg tgatcgatga caactccccc gacggcaccg
     3721 gcgagctcgc cgaccggctc gcccaggagc tcggctacgt cgacgtcctc caccgcgagc
     3781 gcaaggaggg tctcgggccc gcctatctgg ccggcttccg acatgcgctc gccggcgacg
     3841 cggagctcgt gctggagatg gactgcgact tctcgcacga tccggactac gtcccgcgcc
     3901 tgatcgcggc tgtcgaagac ggggccgatc tcgcactcgg ctcgcggtac gtcccgggtg
     3961 gcggcgtacg gaactggggt ctgctgcgcc gcctcatctc tgccggcggt tccttctatg
     4021 cgcgaatcgt cctgggcgtg aaggtccgcg acctgacagg cgggttcaag tgctatcggc
     4081 gtgcggtgct cgaggcgatc gatctcgacg ccgtcgactc gaagggctac gccttccaga
     4141 tcgagacgac ttaccgcgca ctgcgtgccg gcttcgaggt cgtcgaggtt ccgatcacgt
     4201 tcgccgaccg tgaggtcgga ggctcgaaga tgtcgaaggc gatcgtcgcg gaggcgatct
     4261 ggaaggtgcc cggcctccgg ctcaaggccc tgctcggccg tatctaggcg ccgcttgcgg
     4321 tgccgactcc aggaggcgcg ggctttgccc gtgcctccgt ctgaaacgac cggtcgaccg
     4381 aattgacctc aggcacaacc gcggctagga gctacgctga agccatgttc gaggtcaccg
     4441 acgacacgtt cgagcaggac gtcctggggg ccgacacgcc ggtcgtgctc gacttctggg
     4501 cgccgtggtg cggaccgtgc aaagctgtcg agcccattct cgagcaactc gagtccgaca
     4561 cccgtgggcg ggtcgagttc gccaagctga acatcgacga gaacccgctg acggctgcgc
     4621 gctacgacgt gctctcgatc ccgaccgcga tcctgttcga cggcggcgag gccaaggcga
     4681 cggtgatcgg cgcccggccg cgctcgcact acgagcgcgt gctagcggaa gtgctgccag
     4741 ccgcctagct cgtacggctc gccgttcagc gtgatcgcaa cgcctgcgcc ttcctcgcag
     4801 cgtccgatcg ccgtgaagcg gcgcggctcc gagaccgcgg cgagcaactc gtagtcctcg
     4861 ccgaagccga gatcctgcag ctcggctcca ctcgcaagtg gaactcgctc gagctcgacg
     4921 acgacgcggc agcccgagcg cgaggcgatg tggccggcgt cgacggcgag cccgtcggag
     4981 acgtcgagca gggcgtgcgc gtccgccgcg agctccctcc cttcggcgag cctgaccggc
     5041 ggccgcacgt aggactgtcg gcgaaacgcc gcacccgcgc cgccaagcgg gcccgtgagg
     5101 accagttggt ctccggac
//
LOCUS       DASVEU010000048         8741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_810755,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000048 DASVEU010000000
VERSION     DASVEU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810755"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..742
                     /locus_tag="VF225_01845"
     CDS             146..742
                     /locus_tag="VF225_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144024.1"
                     /translation="MSLQQPDFGVLRKAQKGDERAFSLIVRAYQVPVFNYVLRLVGDR
                     SLAEDLTQEVFLRVFQGLPRFSLRSKFTTWLFQVTKNRVLDELRANERRPRAVVALED
                     VPPLEVVDAPMERVEAIDAVWRAVEGLNVDLKMALLLRDIVGLSYTEIADSLEITLAT
                     VKWRIYKAREDVQLSMAREGITFGDQDQTVAERAAASS"
     gene            complement(743..1819)
                     /locus_tag="VF225_01850"
     CDS             complement(743..1819)
                     /locus_tag="VF225_01850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7144025.1"
                     /translation="MTETSLAAGRYRVERTLGQGGMAVVYLAHDEELHRLVAVKVLAE
                     HLAGDANFRARFLQESKLASRLSHPNVVQVYDAGETEGSPFIVMEYVGGDTLAQRGKL
                     THAEVLPLALQACAGLQHAHNAGLVHRDVKPANLLVREDDVLKIADFGIARAAELTRL
                     TQHGTVLGTAAYLSPEQAAGEEVSSATDIYSLGAVVYELLTGRPPYEFESLADLGAQQ
                     TDGVITPLRDVEPSVPKPLEAAVMHALARDPRFRPASASDFAYELASASELPTKPSLA
                     TASTEPPHARTYRSLPGSRAWLWIVGAAAVALVAVILGLLSIGGGSSSARKPQPLPIR
                     SPARGATPAAEARNLSAWLRAHSK"
     gene            complement(1816..2064)
                     /locus_tag="VF225_01855"
     CDS             complement(1816..2064)
                     /locus_tag="VF225_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144026.1"
                     /translation="MRSDEVARALTAGDPCGAGSLAQQLQRETIAAINAGRIPAAFQE
                     QLGSTVADLVSRIQCVPVEKEHDRGKHKNKHKKDGEGD"
     gene            complement(2236..2961)
                     /locus_tag="VF225_01860"
     CDS             complement(2236..2961)
                     /locus_tag="VF225_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7144027.1"
                     /translation="MRVAVISDVHANYHALEAVLKEIDVASVDAVWCLGDTVGYGPRP
                     NECCDVVKDRADHCLVGNHDLVVLGELTVSNFNDEAAAAAIWTAEVLTPESRAFLASL
                     KPFGEVDGVDLFHASARDPVWEYVLTEESAHATLELSDAPLVLVGHSHVALAITADDG
                     RVSGGPAPGGTVVTLDGRWLLNPGSVGQPRDGDPRAAWLLLDFQERVAEFRRVPYPIE
                     RTQAEMRERGLPRILAARLERGD"
     gene            2945..4156
                     /locus_tag="VF225_01865"
     CDS             2945..4156
                     /locus_tag="VF225_01865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7144028.1"
                     /translation="MTATRIGRVATLTVAALVWAVAASALWRTKVPADLHLPRLDSQA
                     VFGVDAVQAGVRYERFLDYAWLLGTIAKVLALVVMVRRGPRLARSLGLGRVNAGLITG
                     VLVVTVLWAVSLPFGIATSWWERRHGISKEDWGTIVLSQWRGVLSGTVTAVIVLAIVL
                     LLAKRFARNWWIGAATTLLVLGVIQQFAAPYANRIGTHPIRSAQLAAAVERLEAREHA
                     GDPTVRVQQVHDQTTAANAYAIGIGPSSSVFIWDTMLDGRFTPREVRFVAGHELAHLA
                     RLHIWKGIAWGTLIGIPLLAAIAFATGRRGGLRDPGNVPLGFLTLTVLLLAVTPFTNV
                     VSRRYEAEADWVGLQGTHDPEAAKGLFKGFVRADLADPNPPGWVHVFLDDHPTLLTRV
                     EQAEAFKRLAR"
     gene            complement(4157..4456)
                     /locus_tag="VF225_01870"
     CDS             complement(4157..4456)
                     /locus_tag="VF225_01870"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX7144029.1"
                     /translation="MDDEPQERQLNIHLDPEQMAGVYANFANVTFSAYEFTLTFARID
                     HEMEEGDIPGVVTSRVNMSAQFFQELLAAMQDSFSKWSTQEGIRNLPEAPPRSDG"
     gene            4477..4641
                     /locus_tag="VF225_01875"
     CDS             4477..4641
                     /locus_tag="VF225_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144030.1"
                     /translation="MEHAFGFSQFAYAVSAMGLVLAGISIFAYIGSLVVEAIARGVIG
                     GCGQSEGGEE"
     gene            4729..6237
                     /locus_tag="VF225_01880"
     CDS             4729..6237
                     /locus_tag="VF225_01880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7144031.1"
                     /translation="MPANAVDRLAARLSELAGAPVELERPSDPEHGDYATNIALRTAP
                     QRKQPPRELAAELAERAQALEEIERAEVAGPGFLNLWVTTAWLADALREIGADYGAGS
                     ALEREKIQVELISANPAGPLTVGSARNGAYGDSVARLLDYAGHDVAREYYFNDAGRQV
                     DLFRASVEARRSGEEPPEGGYQGEYITELAALRVDPVEHMLGEIRREVADFRIHIDTW
                     RHQGEIEKHVAEVLPRLDTYEAEGTIWARTSAYGDDKDRPLVRSSDGSYLYFAADVAY
                     VRDKLERGFDLAIYVLGADHHGYVGRLKAAAAMLGYDPERIEVLIYQLVHIVEGGLTK
                     KISKRRGDVVFLHELVEKIGIDAARWYLISRGHDQPIDIDVDLAAERSQKNPVYYVQY
                     AHARIAGILRNAEATSSDVTAPTELEREERDLIKRLAEFPVIAAEAAERRAPHAIPIY
                     AIRVADDFHRFYHEHRVVGSETEAFRLALVRGAQHVIARALDLIGVEAPERM"
     gene            6431..7420
                     /gene="glpX"
                     /locus_tag="VF225_01885"
     CDS             6431..7420
                     /gene="glpX"
                     /locus_tag="VF225_01885"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX7144032.1"
                     /translation="MSARPPDRNLALELVRVTEAAALGAGRWVGRGDKIAADQAAVDG
                     MRAMLDSVSMAGVVVIGEGEKDEAPMLFNGEPVGDGTGPQVDVAVDPLEGTRLTALGQ
                     PNAIAVIAVAERGTMFFPGAAVYMNKIAVGPQGIDAVDINASPTENVKGVAKAKGVST
                     REITVVVLERERHEGLIAELRTAGAKVLLIRDGDVAAAIAAAQSGTGVDLLYGIGGTP
                     EGVISAAALKCVGGGIQGKLWPRDDEERQQLVDAGLDPERVLTTNDLVAGDDVFVAAT
                     GVTTGSLLRGVRYLPNGAVTDSIVMRSRSGTSRRVEATHALDKLQALTGLEYH"
     gene            complement(7589..8617)
                     /locus_tag="VF225_01890"
     CDS             complement(7589..8617)
                     /locus_tag="VF225_01890"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7144033.1"
                     /translation="MDFQKLEKTAEQIVAEGKGILAADESSGTIKKRFDSIGVESTEE
                     ARRQYRELLFTTEGAEEFIGGVILFDETIRQKASDGTPFPKLLESKGIVPGIKVDTGA
                     KPLALAEGETVTEGLDGLRERVAEYRELGARFAKWRAVITIGKDIPSEYCIWTNAHAL
                     ARYAAICQEGGLVPIVEPEVLMDGDHTIERSFHVTSQTLHAVFTELRDQRIHPEGILL
                     KPNMVLPGYESSEQVSDEEIARQTVQCFRRHVPAAVPGIVFLSGGQSEEEATRRLNAI
                     NAIGPHPWKVSFSYGRALQAAALKAWGGHEENVEAAQRAYYHRAKMNSAAQTGTYTPE
                     LERDAVPA"
BASE COUNT         1354 a         2934 c         2934 g         1519 t
ORIGIN      
        1 accaccaagt gaccctcgaa ggcacaggcg agttggccga tttagtgccc actgtgctgg
       61 ggtttttagg tttcacgcaa cccttacaga tgaccggcaa acccttaacg accccggcct
      121 gatccgggct atactccgga accttttgtc acttcagcaa cctgattttg gcgtcctgcg
      181 gaaggcacag aaaggcgacg agcgagcttt ctcgcttatc gtccgtgcct atcaggtgcc
      241 tgtcttcaac tacgtgctgc gacttgtcgg cgaccggtcg cttgcggagg acctgactca
      301 ggaagtcttc ctgcgggtct tccagggcct gcctcgcttc tcgctccggt cgaagttcac
      361 gacctggctc ttccaggtga ccaagaatcg cgtcttggac gagctgcgcg ccaacgagcg
      421 gcggccccgc gcggtcgtcg cgctcgagga cgttccaccg ctcgaagtcg tggacgcgcc
      481 gatggagcgg gtcgaagcga tcgacgcggt gtggcgcgcg gtcgaggggc tgaacgtcga
      541 cctgaagatg gcgctccttc tccgcgacat cgtcggtttg tcgtacaccg agatcgccga
      601 ttccctggag atcacgctcg cgacggtcaa gtggcggatc tacaaggcgc gcgaggacgt
      661 tcagctctcg atggcccgcg agggcatcac ctttggcgac caggaccaga ccgtcgccga
      721 gcgcgctgct gcctcctctt aactacttcg agtgagcacg cagccaggca ctgaggttgc
      781 gggcctccgc ggccggcgtt gctccgcgcg ccggtgaccg gatcggcagc ggctgcggct
      841 tcctcgcgga actgctcccg ccgccgatgc tgagcaggcc caggatgact gcgacgagcg
      901 ccacggctgc tgcgcccacg atccagagcc atgccctgct cccgggcagg cttcggtacg
      961 tccgggcgtg cggcggctcg gtgctggcgg tggcgagcga cggcttggtc gggagctccg
     1021 acgcagacgc gagctcgtac gcgaaatcgg atgcagacgc cggtctgaag cgcgggtcgc
     1081 gcgcgagtgc atgcatcacc gcggcttcga gcggcttcgg aacagacggc tcgacgtcgc
     1141 gcagcggcgt gatcacgccg tccgtctgct gcgcaccgag gtcagcgagc gactcaaact
     1201 cgtagggcgg ccgtcccgtc agaagctcgt agacgacggc gccgagcgag tagatgtcgg
     1261 tcgcgctgct gacctcctcg ccggcggcct gttctggcga caggtaggcc gcggtgccga
     1321 gcaccgttcc gtgttgcgtt agccgcgtca gctcggcggc gcgcgcgatg ccgaagtcgg
     1381 cgatcttgag gacgtcgtct tcgcgaacca ggagattcgc cggcttgacg tctcggtgga
     1441 cgagcccggc attgtgcgcg tgctgcagtc ccgcgcacgc ctggagcgca agcggcagaa
     1501 cctccgcgtg cgtcagcttt ccccgttgcg cgagcgtgtc gccgccaacg tattccatga
     1561 cgatgaaggg acttccttcc gtctcgcccg cgtcgtacac ctggacgacg ttcggatggg
     1621 agagccggct agcgagtttc gactcctgca gaaagcgcgc tcggaagttt gcgtcgcccg
     1681 ccaggtgctc ggcgagcacc ttgacagcga cgagacggtg cagctcttcg tcgtgcgcga
     1741 gatagacgac cgccatgccg ccctggccga gcgtccgctc gacgcgatag cggcctgcgg
     1801 cgagagacgt ttcggtcaat cgccctcccc gtccttcttg tgcttgttct tgtgcttgcc
     1861 gcggtcgtgc tctttctcga ccgggacgca ctggatgcga gagaccaggt ccgcgacggt
     1921 cgagccgagc tgctcctgga acgcggcggg gatacgcccg gcgttgatcg cggcgattgt
     1981 ctcccgctgg agctgctgcg cgaggctgcc cgccccgcag ggatcgccgg ctgtcagcgc
     2041 tcgggcaacc tcgtcgctgc gcaaggctag tgctgccgca acgggctggg gaagcctggg
     2101 ctgcggggca gcccgacgct ctgagccgct cccgcaacca gcgagacaaa gcacggccgc
     2161 cagggtcgcg ccgcccagct caggacgtct cgttcgggtc ttcctccgct gcaagcttca
     2221 gggaaccctg cctgatcagt cgccgcgctc gagccgggcc gcgagtatcc ggggaagacc
     2281 ccgctcgcgc atctcggcct gggtgcgctc gatcggatat gggacccggc ggaactcggc
     2341 aaccctctcc tggaaatcga gcagcagcca ggccgcgcgc ggatcgccgt cacgtggctg
     2401 gccgacggag ccgggattga gcagccatcg gccgtccagt gtgaccacgg ttcccccggg
     2461 ggccggcccg ccgctgacgc ggccgtcgtc cgccgtgatc gccagcgcga cgtggctgtg
     2521 cccgacgagg acgagcggcg cgtccgaaag ctcgagcgtc gcgtgcgccg actcctccgt
     2581 cagcacgtat tcccagaccg ggtcgcgcgc gctcgcgtga aacaggtcga cgccatcgac
     2641 ctcaccgaac ggcttcaggc tcgcgaggaa cgcgcgtgac tccggcgtca gcacctctgc
     2701 cgtccagatg gctgcagccg cagcctcgtc gttgaagttg ctgaccgtca gctccccgag
     2761 cacgaccagg tcgtggttcc caacgagaca gtggtcggcg cgatccttca cgacgtcgca
     2821 acactcgttc ggacgcggtc cgtagccgac ggtgtcgccg aggcaccaga cggcgtcgac
     2881 gctcgccaca tcgatctcct tcagcaccgc ctccagcgcg tgatagttag cgtgaacatc
     2941 cgatatgacc gcgacccgca tcggcagagt cgcgacctta accgtcgcgg cactggtgtg
     3001 ggcagtcgcc gcctcggctc tgtggcgcac gaaggtaccc gccgacctcc acctgcctcg
     3061 gctcgactcg caggccgtgt tcggggtcga cgccgtccag gcgggagttc gctacgaacg
     3121 ctttctcgac tacgcctggc tcctcgggac aatcgccaag gtgctggcgc tcgtcgtcat
     3181 ggtgcggcgc ggcccacggc tcgcccgctc gctcgggctc ggccgagtga atgcgggatt
     3241 gatcaccggc gtcctggtcg tgaccgtctt gtgggcggtc tcgctgccgt tcggcatcgc
     3301 gaccagctgg tgggagcgcc gccacggcat ctcgaaagag gactggggga cgatcgtcct
     3361 ctcgcagtgg cgaggcgtct tgagcggcac ggtcaccgcg gtgatcgtgc tcgcgatcgt
     3421 cctgctgctt gcgaagcggt tcgcgcgaaa ctggtggatc ggcgcggcga cgaccctgct
     3481 cgttctcgga gtgatccaac agttcgcggc gccctacgcg aaccggatcg gaacccaccc
     3541 gatccgctcg gcgcagctgg cggccgccgt cgagcggctc gaggcgcgcg agcatgccgg
     3601 tgacccgacc gtacgcgttc agcaggtgca cgaccagacc acggccgcca acgcctacgc
     3661 gatcggcatc ggtccgagta gcagtgtctt catctgggac acgatgctgg acggccgctt
     3721 cacgcctcgg gaggtgcgct tcgtggccgg ccatgagctc gcgcatctcg cacgcctgca
     3781 catctggaag gggatcgcgt ggggcacgct gatcggcatt cctctgcttg cggcgatcgc
     3841 ctttgcgaca gggcgccgcg gcggcctgcg cgacccgggg aatgtgcctc tcggtttcct
     3901 gaccctcaca gtcctcctgc tcgccgtgac tccgttcacg aatgtcgtct cccggcgcta
     3961 cgaagcggag gcggattggg ttggtcttca gggaacgcac gatccagagg cggccaaggg
     4021 gctgttcaag gggttcgtgc gagccgatct cgcggatccg aatccgcctg gttgggtgca
     4081 cgtcttcctc gacgaccatc cgacgctgct cacgcgcgtc gagcaggcgg aagccttcaa
     4141 aaggctggcg cggtaactac ccgtcggacc gtggcggcgc ttccgggagg ttccggatcc
     4201 cttcttgcgt cgaccacttg gagaaggagt cctgcatcgc cgccagcaac tcctggaaga
     4261 actgtgccga catgttcacg cgtgaggtta cgactcccgg gatgtcgcct tcctccatct
     4321 cgtggtcgat gcgagcgaag gtgagcgtga actcgtacgc cgagaacgtg acgttggcga
     4381 agttcgcgta gaccccggcc atctgctccg ggtcgagatg gatgttgagc tggcgctcct
     4441 ggggctcgtc gtccacgtcc caacgatact acgggcgtgg agcacgcctt cggcttcagc
     4501 cagttcgcgt acgcggtctc ggcgatgggc ctggtcctcg cgggcatcag tatctttgcc
     4561 tacatcggct cgctcgtggt cgaggcgatc gcgcgtggag tgatcggcgg ctgcggacag
     4621 agcgaaggag gcgaagagtg atcgaggcgt tgcacgacca ccacatcacc tgcggcttcg
     4681 accgcttcgg ccggcgcgcg gcccaactct ccgcgctgcg cgagacgctt gcccgctaac
     4741 gcggtcgacc ggctggccgc gaggctgtcc gagctcgccg gtgcgcccgt cgagctcgag
     4801 cggccgagcg atcccgagca cggcgactat gccacgaaca tcgcgctacg gacggcgccg
     4861 cagcgcaaac aaccgccgcg cgagctggcc gctgaattgg cggagcgtgc acaggccctc
     4921 gaggagatcg agcgcgccga ggtcgccggg cccggctttc tcaacctctg ggtgacgacc
     4981 gcctggctcg cggatgcctt gcgggagatc ggcgcggact acggcgccgg gtctgccttg
     5041 gagcgggaga agatccaggt ggaactgatc tcggccaatc ccgccgggcc gctgaccgtc
     5101 ggctctgcgc gcaacggcgc gtacggagac tccgtggcgc gcctactcga ttacgcggga
     5161 cacgatgtcg cgcgcgagta ctacttcaat gacgcggggc ggcaggtcga cctcttccgc
     5221 gcgtcggtcg aggcgcgacg aagcggggag gagccacccg aggggggtta tcagggcgag
     5281 tacatcactg agctcgcggc gctcagggtc gatcccgtcg agcacatgct cggggagatc
     5341 cgccgcgagg tcgccgactt ccgcatccac atcgacacgt ggcggcacca gggtgagatc
     5401 gagaagcacg ttgccgaggt tctgccgcgc ctcgacacct acgaggccga ggggacgatc
     5461 tgggcgcgca ccagcgccta cggcgacgac aaggaccgcc cgctggttcg ttcctcggac
     5521 gggtcgtacc tgtacttcgc cgccgacgtc gcgtacgtgc gcgacaagct cgagcgtggc
     5581 ttcgacctcg cgatctatgt cctcggcgcg gaccaccacg gctatgtcgg acggctcaag
     5641 gcggccgccg cgatgctcgg ctacgaccct gagcggatcg aggtgctgat ctaccagctc
     5701 gtgcacatcg tcgaaggcgg tctgacgaag aagatctcga agcgccgcgg cgacgtcgtc
     5761 ttccttcacg agctcgtgga gaaaatcggc atcgatgccg cacgctggta cctgatctcc
     5821 cgagggcatg accagccgat cgacatcgac gtcgacctcg cggctgagcg ctcacagaag
     5881 aatccggtct actacgtcca gtacgcccat gcgcgcatcg cgggcatcct gcgcaacgct
     5941 gaggcgacga gctccgacgt cacggcgccg acagagctcg agcgcgagga gcgcgacctg
     6001 atcaagcggc tcgcggagtt tccggtgatt gcagcagagg cggccgagcg acgcgcgccg
     6061 cacgcgatcc cgatctacgc gattcgggtg gccgacgact tccaccgctt ctaccacgag
     6121 caccgagtcg tcggctccga gacggaggcg ttccggctgg cgctcgtccg cggtgctcag
     6181 cacgtgatcg cacgtgccct ggatctgatc ggagtcgagg cgcccgaacg gatgtagggt
     6241 cccgggccag catgagcgtc gctcgccttc ggctcgctcg aaccatgttt cccccacgag
     6301 ctcccttctt tagggatcgc ctcagccccc gcgccgctga gatcgccgcg tgggccgagg
     6361 agaacacgag gggaaacccc ccggtttccc ccctcgcccc ttccaccgct cattgggcaa
     6421 agagcgcgca atgagcgcga ggccgcccga ccgaaatctc gcgctcgagc tcgttcgtgt
     6481 gaccgaggcg gcggcgctgg gtgcagggcg ctgggtcggg cgcggcgaca agattgccgc
     6541 cgaccaggcc gccgtcgatg ggatgcgggc gatgctcgac tcggtctcca tggcgggcgt
     6601 cgttgtgatc ggcgagggcg agaaggacga ggcaccgatg ctcttcaacg gcgaaccggt
     6661 cggcgacggc accgggccgc aggtagacgt cgcggtggac ccactcgagg gaacgcggct
     6721 gaccgcgctc ggccagccga atgcgatcgc cgtcatcgcg gtcgcggagc gcggcacgat
     6781 gttctttccg ggcgccgccg tctacatgaa caagatcgcg gtcgggccgc agggcatcga
     6841 cgccgtcgac atcaacgcgt cgccgacgga gaacgtcaag ggcgtcgcga aagcgaaggg
     6901 agtgtcgaca cgggagatca cggtggtggt gctcgagcgt gagcgccacg aggggctgat
     6961 cgcggaactt cgcacggccg gcgccaaggt cctgctgatt cgtgacggcg acgtcgcagc
     7021 agcgatagca gccgcacaga gcgggaccgg ggtggacctg ctctacggga tcggcggcac
     7081 accggaaggc gtcatctctg cggcggctct caagtgcgtc gggggcggca tccaggggaa
     7141 gctctggcca cgtgacgacg aggagcgtca gcagctcgtc gacgccggcc tcgatccgga
     7201 gcgagtgctg acgacgaatg atctcgttgc aggcgacgat gtcttcgtcg ccgcgacggg
     7261 cgtcaccacg ggctcgctgc tacgcggcgt gcgctacctg ccgaacggcg cggtcaccga
     7321 ttcgattgtc atgcgctcac gatccggtac ttctcgccgt gtcgaggcca cgcacgcgct
     7381 ggacaaactc caggccctga cgggcctcga gtaccactag accactagac ggctactgcc
     7441 tccttctcca tctcaggggc gtacatcccg gttgcgcggc gctgttcatc ttggcgcggt
     7501 ccaaggcgtc gcttgcgatg ccgccgagag tgcgtccggg cttggcccgg gcgcacgtct
     7561 acgctcttga tctaacgctt cgagtgactt aagccggtac tgcgtctctc tccagttcag
     7621 gcgtgtacgt cccggtttgc gcggcgctgt tcatcttcgc cctgtggtag taagcgcgct
     7681 gagccgcctc gacgttctcc tcgtgaccgc cccaggcttt gagcgccgca gcctgcagcg
     7741 cacgaccgta cgagaacgac accttccacg ggtgcgggcc gatggcgttg atcgcgttca
     7801 gccggcgtgt cgcttcctcc tcggactgtc cgcccgagag gaacacgatt cctggcaccg
     7861 cggcgggcac gtgccggcgg aagcactgga ccgtctgacg ggcgatctcc tcgtcggaca
     7921 cctgctcgga gctttcgtac ccggggagca ccatgttcgg cttcagcagg atcccttcgg
     7981 ggtggatccg ctggtcgcgc agctcggtga agaccgcgtg cagcgtctgc gaggtcacgt
     8041 ggaagctccg ctcgatcgtg tggtcgccgt ccatgagcac ttccggttcg acgatcggga
     8101 cgagaccgcc ttcctggcag atcgctgcgt agcgagcgag tgcgtgcgcg ttcgtccaga
     8161 tgcagtactc gctcggaatg tccttcccga tcgtgatcac cgcgcgccat tttgcgaagc
     8221 gcgcgccgag ctcgcgatac tccgcgactc gttcacgaag cccgtcgagg ccttcggtga
     8281 ccgtctcgcc ttcggcgagc gcgagcggct tcgcacccgt gtcgaccttg atgcccggaa
     8341 cgattccctt ggattcgagg agcttgggga acggcgtccc gtcggacgcc ttctgacgga
     8401 tcgtctcgtc gaagagaatc acgccgccga tgaattcctc ggcgccctcc gtcgtgaaga
     8461 ggagctccct gtattgccgc cgtgcctcct ccgtggactc gaccccgatc gagtcgaacc
     8521 gcttcttgat cgtcccgctg ctctcgtccg ccgcaaggat gcccttgccc tcggcaacga
     8581 tctgctcggc cgttttctcc agcttctgaa agtccatctt ccgctcctcc ctcggctttg
     8641 tccggggaga agtatcccct aagctttccg tcttggaggg gcgacgctgc ggcggttcgc
     8701 ccctccctcg gacggggact tggtaagcgc tacaggcgtc a
//
LOCUS       DASVEU010000049         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1395280,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000049 DASVEU010000000
VERSION     DASVEU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395280"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF225_01895"
     CDS             <1..255
                     /locus_tag="VF225_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7144034.1"
                     /translation="AEVQVIASTQALLVAGERRRPKDCGQYQQLEIDYGPFQRQVTLA
                     EPVDPEQATASYERGFLTIRLPVAPEPAPAESISIVVTSS"
     gene            252..2723
                     /gene="lon"
                     /locus_tag="VF225_01900"
     CDS             252..2723
                     /gene="lon"
                     /locus_tag="VF225_01900"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7144035.1"
                     /translation="MSEAMGDTVVEEPTEELTFPATLPVLTLKDTVVFPDSVTPLAIG
                     QERSIKLVEGVVSGDRVLALLTVKTPEAEQPGWDDLYEVGTAAVVHKMIKVPDGTLRI
                     LVQGVQRIKLDHAVQDDPYLVGEFVELPDELPETPEVEALTRNVQSLFGRVIGLVPYL
                     PEELQIAAANVDDPSALCNLVASTLRLKSEEKQRLLELTDVEARLREISAILNRELEV
                     FELGSKIQSQVQEEMEKGQREFFLRQQLKAIQDELGESDPEAAEIAELRSTFESMELP
                     EDVRKVVDRELGRLEKLPTAAAEYGVIRTYLDWIASLPWHKTTEDNLDLDNAKQVLDE
                     DHFDLEKVKERILEHLAVSKLRNDTSGPILCFVGPPGVGKTSLGQSISRALGRKFARL
                     SVGGVRDEAEIRGHRRTYIGAMPGTIIRHLRDAESMNPVMLIDEIDKMGADFRGDPAS
                     AMLEVLDPEQNKNFRDHYLDLPFDLSKTLFICTANTLDTIPAPLLDRMDVIQLSGYTE
                     DEKFGIAKRYLVPKQIEAHGLGGDQVTITDKALRLIIREYTREAGVRNLERRLADVLR
                     KAARQIAEGKRRAKKLRIDEKRVRVSLGPRRYEGEVRKRTSEPGVATGLAFTAVGGDI
                     LFFEATAYPGKGRLTITGQLGDVMQESAQAALSWVRAHTEALGLESDWFREHDIHLHV
                     PAGAVPKDGPSAGITMATAIASLVRNQPVSEDVGMTGEITLTGQVLPIGGIREKTLAA
                     QRAGLKRIIMPRDNEPDLEELPKETKEQLEFILVDTIDQVFDAAFDGKGATHPKPSPV
                     VDRQAAASLKDVSKPSQASVSPPRS"
     gene            complement(2654..3025)
                     /locus_tag="VF225_01905"
     CDS             complement(2654..3025)
                     /locus_tag="VF225_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144036.1"
                     /translation="MARKGAEVQAAQGTSLVSVTLFPLLRRFRPELWTKVIPELDRAA
                     AAVARQQKGKVGDSRTITIAGQKARRYDIAYASEGKQLVERIAFVLRGKSEYLLLCRY
                     ERGGDTEACDGLLTSFKLAAA"
     gene            3204..3626
                     /locus_tag="VF225_01910"
     CDS             3204..3626
                     /locus_tag="VF225_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144037.1"
                     /translation="MTKTRDKVMDRAGGARPYIERAFKDEDLRDNVRNAYESARVVYN
                     ELIGGRGAIPVATRVATDKDIQEHLREALDELRQAADRVQGKKDHGARNTVLLLTGIA
                     LGVLFNPISGAQTRKWLSDTIFGPSDEFTYQGNSGAGS"
     gene            complement(3778..5166)
                     /locus_tag="VF225_01915"
     CDS             complement(3778..5166)
                     /locus_tag="VF225_01915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7144038.1"
                     /translation="MKRAPVAFVAALLLLTAAAADAGWQQARASGATARAYAIRVVVP
                     DQPGAETPTVTAPEDSVVFSGSFDYNKLVTSGSANASASAVAGPAATATASAEVDDLS
                     LFGGEITAKTVVAQAHAEARAGAAKGDIAGSAVTELVALGQPVTGNTLQLADWGSLTT
                     VAGGASGSPATGYRGSVTALEIRLTADHGGLPAGTTVLVGYAEVAAQAAPPEPPIQVP
                     QPPKVGSGTSSKAPEPAPGLPPVRQPPGVTPKLTAGGYVFPVYGPSSFADTFGALRGD
                     ISSGWHHGDDIFAPLGAPLLAVASGTLFSVGWNKVGGNRLWLRDGQGNLFYYAHLSAF
                     SPLAVNGSKVNAGDVVGFVGNTGDAQGTPFHLHFEIHPVGLLGLGYDGAVNPTSYLLA
                     WKHLQDVSFAGGDAWAPLLSLGNAPKPGAILLASTDISTADGLVPGSLQRAMRPLVVR
                     GDNFSSKKKPKK"
     gene            5239..6480
                     /locus_tag="VF225_01920"
     CDS             5239..6480
                     /locus_tag="VF225_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX7144039.1"
                     /translation="MRRRGSEAEIVDEGFWGGSGRRLPLTGSYLVDSLVLAVVILAIA
                     TVIGLALLWPHGKVGRSGQFGPIRTVGAVAEKVESVPCALSASHVCRVAEIRILDGPQ
                     KGHRTLLTTVAAVGSLDVGQGDRIRVYKNTVTPGSSRPSDTYSFADFDRRGAMLWLAI
                     GFVVLLVATGRVHGLRALIGLLASLVIVIKFVVPAILHGSSPFAVAIVGAFAVMLVMM
                     PLSYGLGAKSLAAWLGTAVSLLVAAGLAYAFAHIAHLSGASTDESVYLGASQSRLSLQ
                     GLLVAGMVIGALGVLVDLTVSQASTVIALRRVNPSLGFGGLFRGALEVGHDHIAATVA
                     TLVFAYAGAALPVLLIFSVGGTSFADAVNGEAVADEVIAALVGSIGLIASMPITTALA
                     ALLAPRMSDKQLAAADHAHAH"
     gene            complement(6497..6892)
                     /locus_tag="VF225_01925"
     CDS             complement(6497..6892)
                     /locus_tag="VF225_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144040.1"
                     /translation="MAGPDEIWLPLVDEPVGDIVARLQAEDPEIERLVGSPHRVLAFR
                     TFAYIRVGILLGELLFEQELAVEDADENWVEALLRDPKHHDALHREVRAVAEEIAADP
                     KYSDDEPLGPDEDARDRFREFARKQFAGD"
     gene            complement(6907..7266)
                     /locus_tag="VF225_01930"
     CDS             complement(6907..7266)
                     /locus_tag="VF225_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7144041.1"
                     /translation="MLAVVLGLAAGVLAGMFGVGGGLLFVPTLIILGLNQHEAIGTSL
                     LAIVPTVLVGTWRQSRYGNVRWRAAAVLGVAAAVTAQGGVAVAEALPATTLRRLFAAL
                     LVLVAMQIAWRARHRES"
     gene            complement(7272..7616)
                     /locus_tag="VF225_01935"
     CDS             complement(7272..7616)
                     /locus_tag="VF225_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7144042.1"
                     /translation="MRLVLIGLVAGFFSALFGVGGGIVIVPLLLLIGGWEARSATATS
                     LAAIGVTAVSGVIAYAIHGDVNVGYAALVGLPAAGSVAFATSLQQRLRSGTVELLFAG
                     FMVAVAVWLFVK"
     gene            7634..>7920
                     /locus_tag="VF225_01940"
     CDS             7634..>7920
                     /locus_tag="VF225_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7144043.1"
                     /translation="MDPLDALDRLSEEPGRAALFLDVDGVLAPIVPRPEDARVPEDTR
                     AELRRLNERYALVACISGRDGADAQRVVGIPELVYVGNHGLELDDEAAEWR"
BASE COUNT         1353 a         2672 c         2686 g         1209 t
ORIGIN      
        1 gccgaggtgc aggtgatcgc atcgacgcaa gcgctcctgg tcgccggtga gcgccgccgg
       61 cccaaggact gcgggcagta ccagcagctg gagatcgact acggcccctt tcagcgccag
      121 gtgactctcg ccgagcccgt cgacccggag caggcgaccg ccagctacga gcgcgggttc
      181 ctcacgattc ggctccccgt cgcccccgag cccgctccgg cggagagcat ctcgatcgtg
      241 gtgacctcgt cgtgagcgag gcaatgggcg acacagtcgt ggaggagccg accgaggagc
      301 tcaccttccc ggcgactctg cccgtgctga cgctgaagga cacggtcgtc tttcccgact
      361 cggtgacgcc cctcgcgatc ggccaggagc ggtcgatcaa gctggtcgag ggcgtcgtgt
      421 ccggcgaccg cgtgctcgca ctcctgacgg tcaagacccc tgaggccgag cagcccggct
      481 gggacgacct ctatgaggtc ggcacggccg ccgtcgtcca caagatgatc aaggttccgg
      541 acgggacgct gcgcatcctc gtccagggcg tgcagcgcat caagctcgac cacgcagtcc
      601 aggacgaccc gtatctcgtg ggcgagttcg tcgagctgcc tgacgagctt ccggagacac
      661 cagaggtcga ggcgttgacg aggaacgtcc agagtctgtt cgggcgcgtc atcggcctcg
      721 ttccctacct gccggaagag ctgcagatcg cggccgcaaa cgtcgacgat ccgagcgcgc
      781 tctgcaacct cgtcgcgtcc acgctgcgac tcaagtccga ggaaaagcag cggctgctcg
      841 agctcaccga cgtcgaagcg cgcctccgag agatctccgc catcctcaac cgcgagctgg
      901 aggtgttcga gctcggctcg aagatccagt cgcaggtcca ggaagagatg gagaaggggc
      961 agcgcgagtt cttcctgcgc caacagttga aggcgatcca ggacgagctc ggcgagagcg
     1021 atccggaggc ggcggagatc gccgagctgc gctcgacctt tgaatcgatg gagcttccgg
     1081 aggacgtccg caaagtggtc gaccgcgagc tcggccggct cgagaagctg ccgactgctg
     1141 ccgccgagta cggcgtcata cgcacctacc tcgactggat cgcctcgctc ccgtggcaca
     1201 agacgaccga ggacaacctc gaccttgaca acgcgaagca ggtgctcgac gaggaccact
     1261 tcgatctgga gaaggtgaag gagcggatcc tcgagcatct cgccgtctcg aagctcagga
     1321 acgacacctc ggggccgatc ctctgcttcg taggcccgcc gggcgtcggc aagacgtcgc
     1381 tcggccagtc gatctcacgt gctctcggcc gcaagttcgc ccggctttcg gtcggcggcg
     1441 tccgggacga ggcggagatc cgcggtcacc ggcgcacgta catcggcgcg atgccgggaa
     1501 cgatcatccg tcacctgcgc gacgccgaat cgatgaaccc cgtgatgctg atcgacgaga
     1561 tcgacaagat gggcgcggac ttccgaggcg atcccgccag cgcgatgctc gaggtgctcg
     1621 acccggagca gaacaagaac ttccgcgatc actatctcga cctgccgttc gatctctcga
     1681 agacgctgtt catctgcacg gcgaacacgc tcgacaccat cccggccccg ctcctcgacc
     1741 ggatggacgt catccaactg tcgggctata ccgaggacga gaagttcggc atcgccaagc
     1801 ggtacctcgt gcccaagcag atcgaagctc acgggctcgg cggcgaccag gtcacgatca
     1861 cagacaaagc actccgtctg atcatccgcg agtacacgcg cgaagcgggc gtgcgcaacc
     1921 tcgagcgtcg cctcgcggac gtgctgcgca aggctgcccg gcagatcgcg gaagggaagc
     1981 ggcgcgccaa gaagctacgc atcgacgaga agcgcgtgcg cgtctcgctc gggccgcgcc
     2041 gctacgaggg cgaggtacgc aagcgcacgt ccgagccggg cgtggcgacc ggtctcgcgt
     2101 ttaccgcggt cggcggcgac atcctcttct tcgaggcgac cgcgtatccg ggcaagggcc
     2161 ggctgacgat cacgggccag ctcggcgacg tcatgcagga gtccgcacag gctgcgcttt
     2221 cctgggtgcg ggcgcacaca gaagccctcg ggctcgagtc cgactggttc cgagagcacg
     2281 acatccacct gcacgtaccg gccggcgccg tgccgaagga cgggccctcg gccggaatca
     2341 cgatggcgac tgcgatcgcc tcgctggttc gcaatcagcc ggtctcggag gacgtcggca
     2401 tgaccgggga gatcacgctg accggacagg tgctgccgat cggcggtatc cgcgagaaga
     2461 cccttgcggc gcagcgcgcc gggctgaagc gcatcatcat gccccgcgac aacgaaccgg
     2521 acctcgagga gctgccgaag gaaacgaagg agcagctgga gttcatcctc gtggacacga
     2581 tcgaccaggt cttcgacgcc gcattcgacg gcaaaggcgc gacacacccc aagccgtcac
     2641 cggtcgtcga ccgtcaggcc gccgcaagct tgaaggacgt cagcaagccg tcgcaggcct
     2701 cagtgtcgcc gccgcgctcg tagcggcaga gcagcaggta ctcgctcttc ccgcggagga
     2761 cgaacgcgat ccgctcgacg agctgttttc cctcgctcgc gtaggcgatg tcgtatcgcc
     2821 gagccttttg gccggcgatc gtgatcgtcc tggagtcgcc gaccttgccc ttttgctggc
     2881 gcgcgaccgc ggcggccgcc cgatcgagct cgggaatcac cttggtccag agctccggcc
     2941 ggaagcgacg caacagtgga aacagcgtca ccgagaccag ggacgtcccc tgcgcggcct
     3001 gaacctcggc gcctttccgg gccaccgccc agtcagccgg cgcgacaaac gtaaagccgg
     3061 tcccgcggac gacgtgcccc tttggggccg cacggccccc gcagccagcg aggaccagta
     3121 gagtgagccc gagaacggct tgtttgcgtc gattgcggga gggtacgcag gagctggcaa
     3181 accagcgaaa ggacacagtg agcatgacca agacgagaga caaggtaatg gaccgcgcag
     3241 gcggcgcaag gccctacatc gagcgcgcgt tcaaggacga ggacctgcgc gacaacgtgc
     3301 gtaacgccta cgagtcggcg cgcgtggtct acaacgagtt gatcggcggc cgtggagcga
     3361 ttcccgtcgc cacgcgtgtt gccaccgaca aggacatcca ggagcatctg cgggaagcgc
     3421 tcgacgagct ccgccaagca gcggatcgcg tccagggcaa gaaggaccac ggtgcgcgca
     3481 acacagtgct cctgttgacg ggcattgctc tcggcgtgct cttcaacccg atctcgggcg
     3541 cgcagacacg caagtggttg tccgacacga tcttcgggcc gagcgacgag ttcacctacc
     3601 aggggaactc tggggccgga tcttagggtt cctgcgcccg ctcagcctag acgcgcagtt
     3661 aagacggacg cgcgggcaga gcccgcccgt cctccatggg catcgcaagc gacgccccag
     3721 tcaggggccg ggtcgtaggg tccagcgccg gtcaacctag acggtcggct aagacgacta
     3781 cttcttcggc ttcttcttcg agctgaagtt gtcgcccctc acgacgaggg gcctcatcgc
     3841 acgctgcaac gagcccggaa cgaggccgtc ggcggtcgag atgtcggtcg aggcaagcag
     3901 gatcgccccg ggcttgggcg cgttgccgag ggagagcaac ggcgcccacg cgtcgccgcc
     3961 cgcgaagctc acgtcctgaa ggtgcttcca tgcgagcaga tacgacgtcg ggttgaccgc
     4021 gccgtcgtaa ccaaggccga gcaggccgac gggatggatc tcgaagtgca gatggaacgg
     4081 cgtcccctgc gcgtcgccgg tgttgccgac gaaaccgacg acgtcgcccg cgttcacctt
     4141 gctcccgttc acggcgaggg gcgagaacgc cgagaggtgt gcgtagtaga agagatttcc
     4201 ctgaccgtcg cggagccaga gccggtttcc tccgaccttg ttccacccca ccgagaacag
     4261 cgtcccggac gcaaccgcca ggagcggcgc gccgagcgga gcaaagatgt cgtcgccgtg
     4321 gtgccagccg ctcgaaatgt caccgcgcag cgccccgaac gtgtccgcga acgatgacgg
     4381 gccgtagacg gggaacacgt agccgcctgc ggtcaacttc ggcgtgaccc ccggcggttg
     4441 gcgaaccggc gggaggccgg gggcgggctc cggcgccttc gagctcgtgc cggacccgac
     4501 cttcggcggt tgcggcacct gaatcggcgg ctcaggcgga gcagcctgcg ccgcaacctc
     4561 ggcgtagccg accagcaccg tcgtcccggc aggcagcccg ccgtggtccg cggtgagcct
     4621 gatctcgagg gctgtgacgc tgccccggta gccggtagcc ggggaaccac tcgcgccgcc
     4681 ggcaacggtc gtaagtgaac cccagtccgc tagctgcagc gtgtttcccg tcactggctg
     4741 gcccagcgct acgagctccg tgacggccga tccggcgatg tcgccttttg ccgctcccgc
     4801 gcgggcttct gcgtgggcct gtgcgacgac ggtcttggcc gtgatctcgc ccccgaacaa
     4861 gctcaggtcg tcgacctctg ccgaggccgt cgcggtcgcg gctggacccg cgactgcaga
     4921 ggcgctcgcg ttcgcagagc cgctcgtgac gagcttgttg tagtcgaagc tgccgctgaa
     4981 gacgacagag tcctccggcg cggtgacggt cggagtctcc gcccccggct ggtccggaac
     5041 gacgacacgg atcgcatagg cccgcgcggt cgcccccgaa gcccgagcct gctgccagcc
     5101 ggcatcggcg gccgcagcag tgagcagcaa cagggcggcg acgaaggcga caggcgcgcg
     5161 cttcacgaac gccccattat gcagcctttt cggccggcgg agtcagagcc gtccagggcg
     5221 gtaggcagga tcctccgggt gaggcgccgc ggctccgagg ccgaaatcgt cgacgaaggt
     5281 ttctggggcg gctccgggcg ccggctaccg ctgacgggct catacctcgt cgattcgctc
     5341 gtgctggccg ttgtcatcct cgcgattgcg acggtgatcg gcctcgcact cctgtggccg
     5401 cacgggaagg tcggccgttc tgggcagttc ggcccgatcc ggacagtcgg agcggtcgcc
     5461 gagaaggtcg agagcgtgcc ctgcgcgctc tcggcgtccc acgtctgccg ggtcgcagag
     5521 atcaggatcc tcgatggacc gcagaagggg caccggacgc tcctcacgac ggtcgctgcc
     5581 gtgggctcgc tggacgttgg gcagggcgac cggatccggg tctacaagaa caccgttacg
     5641 cccgggtcgt cgcggcccag cgacacgtac tccttcgccg acttcgaccg caggggcgcc
     5701 atgctctggc tggcgatcgg cttcgtggtg cttttggtgg cgaccggccg cgtccacggt
     5761 ctgcgcgctc tgatcggctt gctcgcgagc ctcgtgatcg tgatcaagtt cgtcgtgccc
     5821 gcgatcctgc acgggtcgtc accgttcgcg gtcgcaatcg tcggcgcgtt cgcggtgatg
     5881 ctcgtgatga tgccgctctc ctacgggctc ggtgcgaaat cgctcgccgc ctggctcggc
     5941 acggcggtga gccttctggt tgccgcaggg ttggcgtacg cgttcgccca catcgcgcac
     6001 ctcagcgggg cctccacgga cgagagtgtc tacctcggcg cctcccagtc gcggctctcg
     6061 ctacagggcc tgctggtcgc cgggatggtg atcggtgcgc tcggggttct cgtcgacctg
     6121 actgtcagcc aagcgtcgac ggtgattgca ctgcggcgcg tgaacccgtc actcggattc
     6181 ggaggcctgt tccgcggcgc actcgaggtc ggacacgatc acattgcggc gactgtcgcg
     6241 acgctcgtgt tcgcgtacgc gggcgctgcg ctgcccgtgc tgctgatctt cagcgtcggc
     6301 gggacgtcgt tcgccgatgc cgtcaacgga gaagccgtcg cggacgaggt gatcgcggcg
     6361 ctcgtgggct cgatcgggct gatcgcatcg atgccgatca cgaccgcgct ggcggcgctg
     6421 cttgcgcccc ggatgtcgga caagcaactg gcggccgccg atcacgctca cgcgcactga
     6481 tctcgacgtg gaacgctcag tcgccggcga actgcttccg ggcgaactcc cggaagcggt
     6541 cccgtgcgtc ctcgtccggg ccgagcggct cgtcgtcgga gtacttcggg tcggcggcga
     6601 tctcttctgc tacggcccgc acctcgcggt ggagggcgtc gtggtgcttc gggtctcgca
     6661 gcagcgcctc cacccagttc tcgtcggcat cctcaacggc gagctcctgc tcgaagagca
     6721 gctcgcccag cagaattccg acacggatgt aggcgaaggt gcgaaacgcg agaactcgat
     6781 gcggcgagcc gacgagacgc tcgatctcgg gatcctcggc ctgtagccgc gcgacgatat
     6841 cgccgaccgg ctcgtcgacc agcggaagcc agatctcgtc ggggcctgcc attcgcacag
     6901 gataggtcag gattcgcggt ggcgcgcgcg ccaggcgatt tgcatcgcga cgagcacgag
     6961 cagcgccgcg aaaaggcgtc gcagcgtggt cgcgggcagc gcctcggcga ctgcgacacc
     7021 gccctgcgcg gtaactgccg ccgccactcc taggactgca gccgcacgcc agcggacgtt
     7081 tccgtagcgc gactgccgcc aggtgccgac gagcacggtc ggcacgatgg cgagcaggga
     7141 ggtcccaatc gcctcgtgct ggttgaggcc caggatgatc agcgtgggaa cgaagagcag
     7201 gccgcccccg acaccgaaca ttccggcgag cacacccgcg gcgaggccga ggacgactgc
     7261 gagcacgtac gtcatttcac gaacagccag acggcgacgg cgaccatgaa accggcgaag
     7321 agcagttcga ccgtgcctga gcgcagccgc tgctgcagcg acgtggcgaa ggcgacggag
     7381 ccggcggccg gcaacccgac gagtgcggcg tagccgacgt tcacgtcccc gtgaatcgca
     7441 taggcgatga cgcccgagac cgcagtgacg ccaatcgccg caagtgacgt tgccgtggcc
     7501 gagcgcgcct cccagccgcc gatcaggagc agcaacggca cgatgacgat gcctcctccg
     7561 accccgaaca gcgcgctgaa gaaccccgcg acgaggccga taaggacgag ccgcacccgc
     7621 gtattcttgc agcgtggatc cgcttgacgc gctcgatcgt cttagtgagg aacctgggcg
     7681 ggccgcactc tttctggatg tcgacggcgt gctcgcgccg atcgttccgc gaccggaaga
     7741 tgcccgcgta ccggaggaca cgcgcgccga gctccgccgc ctgaacgagc gctacgcgct
     7801 cgtcgcctgt atttccggtc gtgacggtgc ggatgcgcaa cgagtcgtag gcattcccga
     7861 gctcgtctac gtcggcaacc acgggctgga gctcgacgac gaggcggctg agtggcgcga
//
LOCUS       DASVEU010000050         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_618699,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000050 DASVEU010000000
VERSION     DASVEU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618699"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..703
                     /locus_tag="VF225_01945"
     CDS             200..703
                     /locus_tag="VF225_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144044.1"
                     /translation="MVALWARAPMADAYSGSTTGRPGGITFERIQGTHYNSCTHTTRY
                     TCFTPWIVGSGPVVSRSPGFGGTQAVLAIYALQVWNGAAWATFGTSRYHTVYLSANSP
                     SARFPRVDFLPKRGGYFRVAISVTWSTLGDVTLGGRAALYNHNADYFCNTRFPCSVGS
                     GSVFLRS"
     gene            complement(847..1242)
                     /locus_tag="VF225_01950"
     CDS             complement(847..1242)
                     /locus_tag="VF225_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144045.1"
                     /translation="MARNVLVVSTVEHADDAIRAEIGDADTVKVVVPAVRQGFLDWLA
                     NDQQAFDRAEVVAERTAEALPGDTVESGAGEADVGLAIRDALATFAADEIVVAIRPDQ
                     QEGFVESLATDNAPQHSVDGVPVRFVVIP"
     gene            1304..>2168
                     /locus_tag="VF225_01955"
     CDS             1304..>2168
                     /locus_tag="VF225_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7144046.1"
                     /translation="MPSEHDPQTAFERLIAATEVLEDVVRDRSLLRALSLEERTRLLT
                     AAADVHNPDVVARRRLNKVVRREKKAAKTRVDETTLAETGIRVLRAQPVFMTPNVFPP
                     HGFEQAEADDVEFRELLEPQHCYVCKRDYSELHPFYDQLCPACGDFNFAKRSETADLS
                     GRVALLTGGRVKIGYQAGIKLLRAGAQLIVTTRFPRDAAARYAEEPDFGDWGERLEIF
                     GLDLRHTPSVEGFCRHLSATRERLDFIVNNACQTVRRPAEFYRHMLEGETASAETMSA
                     PVRALLGEYEGL"
BASE COUNT          344 a          766 c          709 g          349 t
ORIGIN      
        1 aggcactcgg cgactagagg agggtcaggg cttgcagtcg agtaaccctc gcgagcgctt
       61 ctctggcagg gctagaagct ggcgccgcga cgtgatacac acttcaactc cgggtcatgt
      121 gctccgaaag gcgcgtccga agatgctttc aactcgcttg gggggccgac gtttccgtct
      181 tgtgctcatc cttgcgttca tggtcgcgct ctgggcgcgc gccccaatgg cggacgcgta
      241 cagcggctcg accaccgggc ggccaggcgg aatcacgttc gagcggattc agggcaccca
      301 ctacaactcc tgcacgcaca cgacgaggta cacctgcttc acaccgtgga tcgtcggctc
      361 gggcccggtc gtctcgcgca gcccgggttt cggcggtacc caagcagtgc tcgcgatcta
      421 cgcgctgcag gtctggaacg gagccgcctg ggccaccttc ggcacctccc gctaccacac
      481 cgtctacctg agcgccaaca gcccgtctgc ccgcttcccc cgagtcgact tcctccccaa
      541 gagaggcggc tacttccgcg tcgcgatctc ggtcacctgg agcaccctcg gcgacgtgac
      601 gcttggcggg cgcgcggcct tgtacaacca caacgccgac tacttctgca acacgcggtt
      661 cccctgctcg gtcggaagcg gctcggtctt cctgcgcagc tagaggctcg aactctctcc
      721 gttcgcgttc agcgggtcgc tgagctccgc gaaggtcggc tggatcgacg gcgaaaggcg
      781 aacggcgcac aggcgacctg aaggcaaagc tgctggcagt ggcaagctgc tttcggctcg
      841 tcatcgctaa gggatgacga cgaaccggac cggcacgccg tccaccgaat gctgcggcgc
      901 gttgtcggtc gcgagcgact cgacgaagcc ctcttgctgg tcgggtcgga tcgcgacgac
      961 gatctcgtcc gcggcgaacg tggcgagcgc gtcgcggatc gcgagaccga cgtccgcttc
     1021 gccggcaccg gattcgaccg tgtcgcccgg cagcgcctcg gcggttcgct cggcgacgac
     1081 ctcggcccga tcgaacgcct gctggtcgtt cgcgagccag tcgagaaagc cctggcggac
     1141 ggcgggtacg acgaccttga cggtgtcggc gtcgccgatc tcggcgcgga tcgcgtcgtc
     1201 ggcgtgctcc acggtgctga cgacgaggac gttcctggcc acgcccgaaa cctactggct
     1261 cgatcgccca gaccgcagga tcaacgcccg ctaccctcgc tccatgccga gtgagcacga
     1321 tccgcagacg gctttcgagc ggctgatcgc cgccaccgag gtgctggagg acgtcgtccg
     1381 agaccgcagc ttgctgcgcg cgttgtcgct cgaggagcga accaggctcc tcacggctgc
     1441 cgcggacgtc cacaatccgg acgtcgttgc gcggcggcgc ctgaacaagg tcgtgcgccg
     1501 cgagaagaag gccgcgaaga cccgcgtcga cgagaccact ctggccgaga cggggatccg
     1561 cgtcctccgc gctcagccgg tcttcatgac cccgaacgtc ttcccgcccc acggcttcga
     1621 gcaggcagag gccgacgatg tcgagttccg cgagctcctc gagccgcagc actgctacgt
     1681 ctgcaagcgc gactactccg agctgcatcc gttctacgac cagctctgtc cggcctgcgg
     1741 cgacttcaac ttcgccaagc gctcggagac ggccgacctc agcggccgcg tcgccctcct
     1801 cacgggcggc cgcgtcaaga tcggctacca ggcagggatc aagctgcttc gggcgggggc
     1861 gcagctgatc gtgacgacgc gcttcccgcg cgacgccgcg gcgcgctacg ccgaggagcc
     1921 cgatttcggc gactggggcg agcggctcga gatcttcggg ctcgacctgc gccatacacc
     1981 gagcgtcgag ggcttctgcc gccacctgag cgcgacccgg gagcggctcg acttcatcgt
     2041 caacaacgcg tgtcagacgg ttcggcgacc ggcggagttc taccgccaca tgctcgaggg
     2101 cgagaccgcg tccgccgaga cgatgtccgc tcccgtccgc gcgctgctcg gcgagtacga
     2161 ggggctgc
//
LOCUS       DASVEU010000051         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1590945,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000051 DASVEU010000000
VERSION     DASVEU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590945"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF225_01960"
     CDS             complement(<1..371)
                     /locus_tag="VF225_01960"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7144047.1"
                     /translation="MLAHLPVDTSWTFDPLQVVPTVLLAVLYARRVWTLRARGTVVPG
                     WRIALFATGIAVLFAALVSPVHALGEQLFSFHMVQHVLLGDLAPLALLAGLTGPILRP
                     ALAFMHRLRFLAHPFVALPVWA"
     gene            389..1000
                     /locus_tag="VF225_01965"
     CDS             389..1000
                     /locus_tag="VF225_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144048.1"
                     /translation="MQLFHYHLVTSKVREVEARYLGKLGFSLVARHGRIEEDATSFES
                     GHGWDELERIGFKLRLSELERGAVNVVVQPGQWELPRLDHLGFALDDDDFVATLARAE
                     LRELRIQEHGGRRTFVTTNAGYRLELHPPREWLDDFLAGGSDLKLAELQLRADDPELK
                     SAALGELLEVPYTKDTVTIGATVVRFLPDGPQGRPQLHAELFV"
     gene            1135..1470
                     /locus_tag="VF225_01970"
     CDS             1135..1470
                     /locus_tag="VF225_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144049.1"
                     /translation="MVAMVVFFALTFASTAGAEYRIYWWQQNMAPNAQGYDRQTAHNH
                     IYNELYFGPNAGWTSEVWEVTPAGYRHFDKWCAGNCFYAHPGYYYTYSYCSNRDGSTH
                     FVYECKDQW"
     gene            1506..2033
                     /locus_tag="VF225_01975"
     CDS             1506..2033
                     /locus_tag="VF225_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144050.1"
                     /translation="MLVAAAVAGLLTQLGLGGSDKVQIGNGSVSALAQAAQAADTLPQ
                     SVLDYPFAERNFASPNGGGSRLLQEQGSLRLYAVPGKGGMLCLIEVDSAANTAGGACA
                     DRSVLRTGSIYMADRQEDGSRLVVGLVGDGHTYAEADGRRATVENNAFVLNQVEGEDF
                     TVGSPTASQHVDIEG"
     gene            2062..2970
                     /gene="purM"
                     /locus_tag="VF225_01980"
     CDS             2062..2970
                     /gene="purM"
                     /locus_tag="VF225_01980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7144051.1"
                     /translation="MRSKPKTYQGAGVSLATAESIVERLRTAAESTGARAFGGFAGLY
                     PVDEHRLLAASMDSVGSKLMLARRAGRLRWAGMDLAAHCINDVLCTGAVPLFLLDYVA
                     ANHIDKAQVVELVEGAAEVCRAAGCVLIGGETAELPGIYQESELDFAGTCVGLVERDR
                     VIDGSRCEAGDTIIGFTSSGLHTNGFSLVRSLVGDGDFDGDVLLAPHTLYLDEIRSLQ
                     ERADVRALAHVTGGGLIGNLSRVLPDGLEARIDWDSWERPPVFGWLAEQGVEEDELRR
                     VFNVGIGMCAVVPEAPADALVIGELA"
     gene            2967..>3257
                     /locus_tag="VF225_01985"
     CDS             2967..>3257
                     /locus_tag="VF225_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX7144052.1"
                     /translation="MIGVLVSGEGTNLQALIDAGLPIAAVASNRADAGALERADAVGI
                     PNRVFELDGYPDRAARDRDVADWLQLRGVDFVVLAGYMHLLTPGFLVRFRDRI"
BASE COUNT          564 a         1048 c         1130 g          515 t
ORIGIN      
        1 gcccacacgg gcaaggcaac gaaaggatgg gcgaggaaac gcaggcgatg catgaaggca
       61 agcgccggcc gcaggatcgg ccccgttagc cccgcgagga gcgcaagggg cgcaaggtcg
      121 cccagcagga cgtgttggac catatggaag ctgaagagct gctcgccgag cgcgtgcacg
      181 ggactgacga gcgcggcgaa gaggacggcg attccggtcg cgaagagtgc gatccgccag
      241 cccggaacca ctgtcccccg ggcgcgtagc gtccataccc ggcgcgcata aaggaccgca
      301 agcaggaccg tgggcaccac ctgaaggggg tcgaaagtcc agctggtgtc gacgggcaga
      361 tgggccagca cggctctagt ctgccgtcat gcagctcttc cactaccacc tggtgacgag
      421 caaggtgcgg gaggtcgagg cgcgctacct cggcaagctc ggcttcagcc tggtcgccag
      481 gcacggccgg atcgaggagg acgcgacctc cttcgagtca gggcacggct gggatgagct
      541 ggaacggatc gggttcaagc tgcggctgtc ggagctcgag cggggcgccg tgaacgtcgt
      601 cgtccagccg ggccaatggg agctgccgcg tctggatcac ctcggcttcg cgctcgacga
      661 cgacgatttc gtcgccacgc tggcgcgagc cgagctgcgt gagctgcgca tacaggagca
      721 cggcggcagg cgtacgttcg tgacgacgaa cgcgggctat cggctcgagc tgcatccgcc
      781 gcgcgagtgg ctcgacgatt tcctcgccgg tggttccgac ctcaagctcg cggagttaca
      841 gctacgcgca gatgaccccg agctgaagtc ggcggcgctc ggcgagctgc tcgaggtgcc
      901 atacacaaag gacaccgtga cgatcggagc gacagtcgtt cgcttccttc ccgacggacc
      961 gcaaggccgt ccgcagctgc acgcagagct gttcgtctaa gccgtagctg cacgcgacac
     1021 tgtccgaaag agcgaattga cgggaagcct cgttcgccgg atgcttgcgc gagtttggaa
     1081 acctcgacaa ggacggtgca agtgcgactg agattccgaa gacggtgggc agtcgtggtg
     1141 gcgatggtcg tgttcttcgc cctgacgttc gcatccactg caggcgcgga ataccgcatc
     1201 tactggtggc agcagaacat ggccccgaac gctcagggct acgaccgcca aacggctcac
     1261 aaccacatct acaacgagct ctatttcggg ccgaacgcgg gctggacgag cgaggtctgg
     1321 gaggtcacac cggccggtta tcgccatttc gacaaatggt gcgccggtaa ctgcttctac
     1381 gcgcacccgg gctactacta cacctactcg tactgctcga accgtgacgg cagcacgcat
     1441 ttcgtctacg agtgcaagga ccagtggtaa ccgtgagaga tagacgcgtt caattcggag
     1501 cggccgtgct ggtcgcggcg gcggtcgccg gcctgctcac gcaactcggt ttgggcggca
     1561 gcgacaaggt gcagatcggc aacggttcgg tgtccgcgct cgcacaggcg gcacaggcgg
     1621 ccgacacatt gccccagagc gttctggact atcccttcgc ggagcggaac ttcgcttcgc
     1681 cgaacggtgg cggctcgcga ttgctccagg agcagggctc cttgaggctc tacgccgtgc
     1741 cgggcaaagg agggatgctc tgcctgatcg aggtggactc ggcggccaac acggcaggcg
     1801 gggcttgtgc cgatcgcagc gtcctccgga ccggcagcat ctacatggcc gaccgccagg
     1861 aggacgggtc ccggctcgtc gtagggctcg tcggggacgg ccacacgtat gcagaagccg
     1921 acggccggcg ggcgaccgtc gagaacaacg ccttcgtgct gaaccaggtg gagggcgagg
     1981 acttcacagt gggatctccc actgcgagcc agcacgtcga catcgaaggc tgacgccggt
     2041 cgcggctcta gacttggccc cctgaggagc aagccgaaga cgtatcaggg ggccggcgtc
     2101 tcgctcgcca cggccgagtc gatcgtcgag cgcctgcgga ccgctgccga gtccacgggc
     2161 gctcgggctt tcggcggctt cgcagggctc tacccggtcg acgagcaccg cctcctcgca
     2221 gcatcgatgg acagcgtcgg ctcgaagctg atgctcgcgc ggcgcgccgg ccgcctgcgc
     2281 tgggccggga tggatctcgc agcccactgc atcaacgacg tgctctgcac aggagcggtc
     2341 cccctcttcc tgctcgacta cgtcgcggcg aaccacatcg acaaggccca ggtcgtcgag
     2401 ctcgtcgagg gggcggccga ggtttgtcgg gcggccggct gtgtgctcat cggaggggaa
     2461 acggccgagc tgccggggat ctaccaggag tcggagctcg acttcgccgg gacatgtgtc
     2521 gggctcgtcg aacgcgatcg tgtgatcgac gggtcccgct gcgaggccgg cgacacgatc
     2581 atcggcttca cctccagcgg gctgcacacg aatggcttct cgctcgtacg gtcgctcgtc
     2641 ggcgacggcg acttcgacgg cgacgtgctg ctcgcgccgc acacgctcta cctcgacgag
     2701 atccgcagcc tccaggaacg tgccgatgtg cgcgcgctcg cgcacgtgac cggcggcggc
     2761 ctcatcggaa acctctcccg cgttttgccc gatgggttgg aagcgcggat cgactgggac
     2821 tcgtgggagc gcccaccagt cttcggctgg ctcgccgagc agggagtcga ggaggacgag
     2881 cttcggcgcg tcttcaacgt cggcatcggg atgtgcgccg tagtcccgga agcgcccgcg
     2941 gacgcactcg tgatcggcga gctcgcatga tcggcgtgct cgtctccggc gagggaacga
     3001 acctgcaggc actcatcgat gcggggctgc cgatcgcagc cgtcgcgtcc aaccgcgcgg
     3061 atgccggcgc gctcgagcgt gccgacgccg tgggaatccc aaaccgggtc ttcgaactgg
     3121 acggctatcc cgaccgcgcg gcgagagatc gggacgtcgc cgactggctg cagctgcgcg
     3181 gcgtcgactt cgtcgtgctg gccggctaca tgcacctgct cacgcccggg tttctcgttc
     3241 gcttccgcga tcggatc
//
LOCUS       DASVEU010000052         9757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_427120,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000052 DASVEU010000000
VERSION     DASVEU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427120"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VF225_01990"
     CDS             complement(<1..754)
                     /locus_tag="VF225_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144053.1"
                     /translation="MITAKQHEGQRSDGTYTSGNITQYKEGDTINFRFDLTGSDTSSG
                     QLQVRFTENDGTCLFFANYFVLGAVTNVDGATPTVGVASGPTADAGEWVVTLNIDFAA
                     AGEAIVNYQLKLSDQAGDCNGSSQHSRLNPGDGVSQTGQQNVPVPANQIIELPNITVA
                     KLVDRADGAGFVPAAAGEYCFMLDSGACTPIDSSGQVVFVNVADGAHTITESSNLGHP
                     GYTFDSGVGTNCTFAGSTATATVAQGTTATNAS"
     gene            1165..1851
                     /locus_tag="VF225_01995"
     CDS             1165..1851
                     /locus_tag="VF225_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144054.1"
                     /translation="MPRVLLRALATVLALFSISFAAPGRAQAACGSSGYAYAGLGANH
                     RAYGVATWLQAVAAPEVLSGHVAGWVGVGGPGAGPNGTDEWLQVGFSAFPGASTSSLY
                     YEVAQPHQAPQYHEVALGVPTGSIHKVAVVEIRNRRSWWRVLVDDHAVGEPVYLPGSH
                     GAWRPMATTETWGAGSFVCNRFSYRFGSVSVATRPGGGWQQLRSHYTFQNHGYRIRSL
                     GAATFLAGAL"
     gene            complement(1857..2567)
                     /locus_tag="VF225_02000"
     CDS             complement(1857..2567)
                     /locus_tag="VF225_02000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7144055.1"
                     /translation="MTTTRFFATWALAAFLLATTFVGGASAIAPALPASYPAAGELVA
                     TRVAVRTSPDRGAKVVRLLQQFRPDYRLQIVLAVGQASAPDGSVWFKLNLPMRPNGTV
                     GWIPAASVTLRPTQSRIVVHRGLRRIELFRDGKRLLHAKVAVGAPGRETPLGEFYVTA
                     RFVPDDPFLGSFALETSAYSRLTEWPGGGKVGIHGTSKPWLLGQAVSHGCVRVSNQTA
                     LALRRLAPVGTPISIVAR"
     gene            complement(2620..3075)
                     /gene="greA"
                     /locus_tag="VF225_02005"
     CDS             complement(2620..3075)
                     /gene="greA"
                     /locus_tag="VF225_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7144056.1"
                     /translation="MASDKSDALTAAQKAQLEAELAELEGPRRTEAVQAIATARSFGD
                     LSENFEYHAAKNEQGLLERRITMLRDRLERSVLIDESDLDGDTVGVGTKVEIEDEEGE
                     RMEVEISSVGGVSPDSPVGRALMGRKVSDEVEIQAPSGAWRARILSIGR"
     gene            3227..4396
                     /locus_tag="VF225_02010"
     CDS             3227..4396
                     /locus_tag="VF225_02010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7144057.1"
                     /translation="MHQSFRELGVSPPVVDALAARSIHEPFRIQALVLPDALAGLDVL
                     AKSPTGSGKTLAFAVPIVERTTTADARPSALVLVPTRELAAQVSAELESLVPAKGLKV
                     ASVYGGVPLNAQAKRAKSAHVLVATPGRLQDLAERRLVDLSHVRILVLDEADRMLDMG
                     FQPQVDRIVRRLPRNRQTMFFSATLDGPVGELARNYTNSPSRFEAALAEEQEPGEIEH
                     QFVSVTADTKVETLVKQLNSSNGLTLVFVRTKRGADKLVQKLARHDVRAVAMHGDMSQ
                     GARERALSRFESGKVTTLVATDVAARGLDLDDITHVINFDPPGEDKDYVHRTGRTGRA
                     GRSGKAITLVLPEQQAETSHVARRLGHRAQFEEAGLQSARAKLMYTSRRGRGSKW"
     gene            complement(4404..6242)
                     /gene="typA"
                     /locus_tag="VF225_02015"
     CDS             complement(4404..6242)
                     /gene="typA"
                     /locus_tag="VF225_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7144058.1"
                     /translation="MALREDIRDIAIVAHVDHGKTTLVDAMLWQSGSFRANQEVAERV
                     LDSMDLEREKGITILAKNTAVRRGGVKINIVDTPGHADFGGEVERALTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALESRLPVILVVNKVDRPDARIEEVVNEVYELFLDLDADESQI
                     EFPIVYANARAGRAGLEPDDLADDLDPLFDVLLERIPAPFYEEGHPMQAHVTNLDASP
                     YLGRLALCRIREGTIRKGQQIAWCRANGEITRVKVTELFVTEALDRVEAEEAGPGELI
                     AVAGIPEVTIGETLADPDDPRPLPVIGVDEPSLAMTIGVNTSPLAGQDGTRLTASMVL
                     ARLEQELVGNVSLRVLPTERPDAWEVQGRGELQLAILVELMRREGFELTVGKPQVVTR
                     EVDGKVHEPMERLAIDAPEDYVGVITQLLALRKGRLEQMVNHGTGWVRMEYVVPARGL
                     IGFRTEFLTETRGTGILHHVFHAYEPWHGELRMRPTGSLVADRRGPATAFALLNLQER
                     GQLFIGPGTEVYEGMVVGENARAEDLDVNATKEKKLTNMRSSTADELVRLIPPRLLSL
                     EQSLEFLGDDECVEVTPKNVRLRKAELSGQKRQTATSRKARGLAKV"
     gene            6349..6639
                     /locus_tag="VF225_02020"
     CDS             6349..6639
                     /locus_tag="VF225_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144059.1"
                     /translation="MTADARSVQRVEGGRINGDMLWFNNEKGHGFIETAGGDRLRVEG
                     GDFRNGPPEGRVAGMAVTFQVIGDGDSARAQDVVFPDDAAPRRARIRRSAGR"
     gene            complement(6812..7852)
                     /locus_tag="VF225_02025"
     CDS             complement(6812..7852)
                     /locus_tag="VF225_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7144060.1"
                     /translation="MTFERPLLLLTLLVVPLAVALYVLAERRRMRYAIRFTNLDVLAS
                     VTGGRYRRRFIPLALFFLALAALCIGMARPRHTTLVPRDRATVILVIDVSRSMEAKDV
                     KPNRIGAAAAAVRTFLDRVPDRLQVGLIAFAGDPAVASPPTTNHDLVRQSLDTIEWFP
                     SFGGTAIGDALAAAVKLGQQAVGRPSGNLASATTAAPETQTQGLVSILFLSDGAQTRG
                     DLEPLEGADLAKAAGIPVYTVALGTPNGVLNFGPGGGGGGGGFPGQFGGRRVPVPPDP
                     ETLRAIAVRTGGQFFAAQSAKSLQSAYSKLGSRLGRKPGQSEITYAFLAAAALLLVTA
                     GLLSALWSPRLP"
     gene            complement(7849..8508)
                     /locus_tag="VF225_02030"
     CDS             complement(7849..8508)
                     /locus_tag="VF225_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144061.1"
                     /translation="MSASSAGATNECRGLPVCVRVAGPWVVVPAAQTTPRPRVDFQLS
                     CPRGYVIGGLDAELTDRAIDIDFLGKLGSPVNPGVTTARSALFRGTYTGNAPKGPSFR
                     PHLGCLPSAGGGSGPVPRRALAAFSPGEPTTNRVRTVRLRPGLVHAVASCAGSERLIS
                     AWHAVAFYTQSAPPQELIQSVSATRSVHGRRVEVRVRSTAAVQAVRAVVQVGAVCGGG
                     S"
     gene            complement(8553..9515)
                     /locus_tag="VF225_02035"
     CDS             complement(8553..9515)
                     /locus_tag="VF225_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7144062.1"
                     /translation="MSFQSPLALIGLVLVPVLVGLYLLRERRRERYATRFTAPALLPN
                     LVAAAPGWRRHLPVALFLVALAAMIVGVARPHASVSVQREEATVLIAIDSSLSMSAQD
                     VRPSRLAAAQSAAQAFVDGMPKKFRVGVIGFAGRAYVAVPPTEDRELVHSALKALKPG
                     EGTALGDAVALGTRLARAERTSDGRIPPTAMLVISDGAQMSGRTTPQVAATQAKSAHI
                     PVYTVVVGTPDGVVNVPVAGGYQAQLRVPPSPETLRTVASMTAGQFFTAPDATRLRQI
                     YEKLGSRLGHRRQKREITDLFAGGSAAFLVFGGALSALWFRRVP"
     gene            complement(9512..>9757)
                     /locus_tag="VF225_02040"
     CDS             complement(9512..>9757)
                     /locus_tag="VF225_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144063.1"
                     /translation="PREQELPNAGVLWLVDPETGRQLRADTRSEQLRARFAAAAAAER
                     AELARTLAAAGARHLVLTTAGDWLRSFAVFLRRDARR"
BASE COUNT         1615 a         3328 c         3225 g         1589 t
ORIGIN      
        1 acgaagcgtt cgtcgccgtc gtgccctgcg cgacggtcgc ggtcgccgtg ctgccggcga
       61 acgtgcagtt cgtgcctaca cccgagtcga aggtgtaacc cgggtgcccg agatttgagc
      121 tttcggtgat ggtgtgagct ccatcagcca cgttgacgaa gacgacctgc ccgctggagt
      181 cgatcggcgt gcaggcgccg gagtcgagca tgaagcagta ctcacccgcg gcggcgggga
      241 cgaaccccgc gccgtctgcg cgatcgacga gtttcgcaac ggtgatgttc gggagttcga
      301 tgatctggtt cgccggaacc gggacgttct gctggccggt ctgcgaaacg ccgtcccccg
      361 gattgaggcg gctgtgctga gacgatccat tgcagtcgcc ggcctggtcc gagagcttga
      421 gctggtaatt gacgatcgct tcaccggcgg cggcgaagtc gatgttgagc gttacgaccc
      481 actcgcccgc gtcggcggtg ggcccggagg cgacgccgac cgtcggcgtg gcgccatcaa
      541 cgttcgtgac cgctccgagg acgaagtagt tcgcgaagaa gagacaggtg ccgtcgtttt
      601 cggtgaaacg cacctgcagt tgaccggagg aagtgtcaga tccggtgagg tcgaagcgga
      661 agttgatcgt gtcgccttcc ttgtactgcg tgatgttccc agacgtgtac gtcccatcgc
      721 tgcgctgacc ctcatgctgc ttcgcagtga tcacttgcga agcggtgaga tgcctcggcg
      781 agctattgcc gaaccgcatc gcggaggacg cgccggcggt gatcgccatc aaaacaagca
      841 gcgcgacgag cggaatgccc cacttggccc atgttcggct ggatctgttg ttgcctctca
      901 ctcgagcttc cccctcgtct tgattgcacc gacgactagc gcgccctgag gcgcaatctc
      961 cccacatggg taatagcccg ggtaccggcg agctggcact ggcgagaggg ctaggtcatc
     1021 tagtcccttt ggcgaaaggc cgtcctgatc gcgccaagct gccgaaggcg gcgatcactc
     1081 gaacgaggga cgcctggccg agcccggccg tccgattgaa caagcgacgg atttttgtcg
     1141 caggccgctc attcggaggt ttccgtgccc agggtgcttc tccgagcgct ggcaacagtg
     1201 ctcgctcttt tctcaatcag ctttgccgcc cccggcagag cgcaggcagc ctgcggctcg
     1261 agcggctacg cctatgcagg tctcggcgcg aatcaccgcg cttacggcgt ggcaacctgg
     1321 ctccaggcag tcgcggcgcc cgaggtgctc agcggacacg tcgccggctg ggttggcgtc
     1381 ggagggccgg gcgccggtcc gaacggaacc gacgagtggc ttcaggtcgg cttcagcgcg
     1441 ttcccggggg cctcgaccag cagcctgtac tacgaggtcg ctcagcccca ccaggcaccc
     1501 cagtaccacg aggtcgctct cggggtccct accggctcga tccacaaggt cgcggttgtc
     1561 gagatccgga atcggcgcag ctggtggcgt gtcctcgtcg acgaccacgc ggttggcgag
     1621 cccgtgtacc tcccgggcag ccatggagca tggcgtccaa tggccacgac cgaaacgtgg
     1681 ggagcgggct ctttcgtctg caaccgcttc agttaccgct tcggatctgt gagcgtcgcc
     1741 acccgtcccg gcggcggctg gcagcagctg aggagccact acacatttca gaatcacggc
     1801 taccgcatca gaagcctcgg cgcggcgacg tttctcgccg gagccctgta gtcgggctaa
     1861 cgcgcgacga tcgaaatggg cgtcccgacc ggcgccagcc gcctcagcgc gagcgcggtc
     1921 tgattggaga cccgaacgca gccgtgcgag accgcctgcc cgagcagcca cggcttgctc
     1981 gttccatgga tgccgacctt gccgccgccc ggccactcgg tcaatctcga atacgcgctc
     2041 gtctcgagcg cgaacgatcc gagaaacgga tcgtccggga cgaagcgtgc tgtcacgtag
     2101 aactctccca gcggagtctc gcggccgggc gcaccgacgg ccaccttggc gtgaagcaac
     2161 cgcttgccgt cccgaaacag ctcgatccgc ctgagcccgc ggtgcacgac gatgcgactt
     2221 tgcgtcggtc tcagcgtgac gctcgcagcc ggtatccacc cgacggtccc gttcggtcgc
     2281 atcggcaggt tgagcttgaa ccagacggag ccgtcgggtg cactcgcctg gccgaccgcc
     2341 aacacgatct gaagccgata gtcgggccgg aactgctgca gtaggcggac gaccttcgca
     2401 ccgcggtctg ggctcgtcct caccgcgaca cgcgtggcga cgagctcacc cgcagccgga
     2461 tacgaggcgg gcagcgcagg cgcgatcgcc gatgctcccc cgacgaaagt cgtcgcgagg
     2521 aggaacgccg cgagcgccca cgttgcgaag aaacgcgttg tggtcatctc agctcaatat
     2581 agagcgagga tcagagatcc acgctctgcc gacgttcgac tagcggccga tcgacagaat
     2641 gcgggcgcgc caagcgccgc tcggcgcctg gatctcaacc tcgtcgctca ccttgcgtcc
     2701 catgagcgcg cgcccaacag gtgagtccgg cgacacgccg ccgacgctgg agatctcgac
     2761 ttccatccgc tcgccctcct cgtcttcgat ctcgaccttc gtcccgacgc cgaccgtgtc
     2821 gccgtcgagg tcgctctcgt cgatgagcac ggagcgctcg agccggtctc gcagcatcgt
     2881 gatgcgccgc tcgaggaggc cctgctcgtt cttcgcggcg tgatattcga agttctcgga
     2941 caggtcgccg aagctccgag cggttgcgat cgcctggact gcttcggtgc gacgggggcc
     3001 ctccagctcg gcgagctcgg cctcgagctg cgccttctga gccgcggtca gtgcatcgga
     3061 cttatcgctt gccataaggg gagcgtatct gccccgttcc acgattagct gctaccttgt
     3121 ggcaacgagt gacattcggt tgctcggtga gatgagcttc tcgttcctcc ttcgggtgtt
     3181 tcgaggtagg caagtctcga acccatagaa ggagtcgaac aactttatgc accagtcatt
     3241 ccgcgagctt ggcgtgtcgc cgcctgtcgt cgacgcgctc gccgctcgct ccatccacga
     3301 gccgttccgg atccaggccc tcgtcctgcc ggatgccctc gcagggctcg acgtgctcgc
     3361 aaaatctccg accggatccg ggaagacgct tgcgttcgcc gtgccgatcg tcgaacgcac
     3421 cacaaccgcc gacgcccgcc cgtcggcact cgttctcgtc ccgacccgcg agctcgccgc
     3481 tcaggtgagc gcagagctcg agtcgctcgt gccggccaag ggcctcaagg tggcctccgt
     3541 ctacgggggc gtgccgctca acgcccaggc gaagcgggca aagagcgcgc atgtcctggt
     3601 cgcaacgccc ggacgcctgc aggatctcgc cgagcgtcgt ctcgtcgatc tctcgcacgt
     3661 tcgcatactc gtcctcgacg aagccgaccg gatgctcgac atgggctttc agccgcaggt
     3721 cgaccgcatc gtccgccggc tgccgcgcaa ccgccagacc atgttcttct cggcaacgct
     3781 agacggcccg gtcggcgagt tggcgcgcaa ctacacgaat agcccatccc gcttcgaggc
     3841 ggctctggcc gaggagcagg agccgggcga gatcgagcac cagtttgttt cagtcaccgc
     3901 cgacaccaag gtcgagacgc tcgtgaagca gctcaactcc tcgaacggtc tgacgctcgt
     3961 cttcgtacgg acgaagcgcg gcgcggacaa gctcgtgcag aagctcgctc ggcacgacgt
     4021 ccgtgccgtc gcgatgcacg gcgacatgtc ccagggagca cgcgagcgcg cgctttcccg
     4081 cttcgagagc ggcaaggtga cgacgctcgt cgccaccgat gtcgcggcgc gcggcctgga
     4141 tctcgacgac atcacgcatg tgatcaactt cgatccgcca ggcgaggaca aggattacgt
     4201 tcaccgcacc ggccggactg gccgcgcggg acgcagtggt aaggcgatca cgctcgtcct
     4261 tcccgagcag caagccgaga cgagccacgt cgcccgccgg ctcggtcatc gcgctcagtt
     4321 cgaagaagca ggcctccagt cggcgcgagc gaagctcatg tacacgagtc gccgcggccg
     4381 gggctcgaag tggtagcggc cgtttagacc ttcgcgagac cgcgcgcttt gcgggacgtg
     4441 gccgtctggc gcttttgtcc ggacaactcc gccttccgca ggcgcacgtt cttgggagtg
     4501 acctcgacgc attcgtcgtc gccgaggaac tccagagact gctcaagtga gagcaggcgc
     4561 ggcgggatca gcctgacgag ctcgtccgca gtggacgagc gcatgttggt gagcttcttc
     4621 tctttggtcg cgttgacgtc gaggtcttcg gcgcgcgcat tttctcccac gaccatgccc
     4681 tcgtagacct cggtgcccgg cccgatgaac aactgcccgc gctcctggag attgagcaag
     4741 gcaaacgccg tcgcggggcc gcgtcgatcg gcgaccagac tgcctgtggg ccgcatgcgc
     4801 agctcgccgt gccagggctc gtacgcgtga aagacgtggt ggaggatgcc tgtgccgcgc
     4861 gtctcggtca ggaactccgt gcggaagccg atcaagcctc gcgcagggac cacgtactcc
     4921 atccgcaccc agccggtgcc gtggttcacc atttgctcga gacgtccctt gcggagggca
     4981 agcagctgag tgatcacgcc gacgtagtcc tccggtgcat cgatcgcgag ccgttccatc
     5041 ggctcgtgca cctttccgtc cacctctcga gtcaccacct gcggcttgcc gaccgtgagc
     5101 tcgaagcctt cgcgacgcat cagctcgacg aggatcgcga gctggagctc cccgcgccct
     5161 tgcacttccc aggcatccgg ccgctcggtc ggaagcacgc ggagggagac gttcccgacg
     5221 agctcctgct cgagtcgcgc gagcaccatg ctcgcggtga gtcgtgtgcc atcctggccc
     5281 gcgagtggag aggtgttgac gccgatcgtc atggcgaggc ttggctcgtc cactccgatc
     5341 accggcagcg ggcgcggatc gtccggatcg gcaagcgtct cgccgatcgt gacctcggga
     5401 atcccggcga cggcgatcag ctcgccgggc ccggcctcct cggcctcgac gcggtcgagc
     5461 gcctctgtga cgaagagctc ggtgaccttg acgcgcgtga tctcaccgtt ggcgcggcac
     5521 cacgcgatct gctgaccctt gcgaatcgtc ccttcacgga tacggcagag cgcgagccgc
     5581 cccaggtacg gagacgcgtc caggttcgtg acgtgcgcct gcatcgggtg cccctcctcg
     5641 tagaaaggtg cgggaatgcg ttcgagtagc acgtcgaaga gtggatccag gtcgtcggcg
     5701 agatcgtcgg gctcgagccc ggcacggccg gcccgtgcgt tggcgtacac gatcggaaac
     5761 tcgatctggc tctcgtccgc gtcgagatcg aggaagagct cgtacacctc gttcacgacc
     5821 tcctcgatgc gcgcatccgg ccggtctacc ttgttgacga cgaggatcac gggcagccga
     5881 gactcgagcg ccttgcgcag gacgaacctc gtctgcggca gcggcccctc gctcgcgtcg
     5941 acgagcagca agacgccgtc gaccatcgtc agcgcgcgct cgacctcgcc gccgaagtcg
     6001 gcgtgtcccg gcgtgtcgac gatgttgatc ttgacgccgc cgcgacggac ggccgtgttc
     6061 ttcgcgagga tcgtgatccc cttctcgcgc tcgaggtcca tcgagtcgag cacgcgttcg
     6121 gctacctctt gattcgcccg aaaggaaccc gactgccaca gcatcgcgtc cacgagcgtc
     6181 gtcttcccgt gatcgacgtg ggcgacgatc gcaatgtccc gaatatcctc gcgaagcgcc
     6241 attcgacgag ggtagcggca cggaccgccg cccctgtgga tttcggcgct atcctgtgtc
     6301 tcgactggag cgagcatctg cgcgcgcccc tttgaggagg atgattccat gacggcagac
     6361 gctcgatcgg tgcagcgcgt cgaaggtggt cggatcaacg gcgacatgct gtggttcaac
     6421 aacgagaagg gccacggctt catcgagacc gcgggcggag atcgcctgcg cgtcgagggc
     6481 ggcgatttcc gtaacgggcc gcccgaagga cgggttgccg gcatggcggt cacgttccag
     6541 gtcatcggtg acggcgactc ggcaagagcg caggacgtcg tctttccgga cgatgccgca
     6601 cctcggcgcg cgcggatccg ccgcagcgcc ggccgctgaa acgcgctatc gcgtgacaaa
     6661 cgcgtcacgg ttgcgtaccc gtgcctggca ccggaacgtc cccgcgagtt gcagcgcatt
     6721 cgtggctttc gtcactcttc ggtgaccgtt gcgtacccgt gcctggcacc ggtacgcggc
     6781 ggaagcgggc acgcctgagt tggatccgtg cctacggcag gcggggcgac cagagcgcgg
     6841 acagaaggcc tgccgtgaca agcagcaatg ccgcggccgc caggaacgcg taggtgatct
     6901 cgctctggcc gggcttgcgc ccgagacggg agccgagctt cgagtacgcc gattgcaacg
     6961 acttcgcgct ctgcgcggcg aagaattggc ccccggtacg cacggcgatc gccctcagtg
     7021 tctcgggatc gggcgggacc ggcacccgcc ggccgccgaa ctgacccgga aagccgccgc
     7081 cgcctccgcc tccgccgggg ccgaagttga gcacgccgtt cggcgtgccg agcgccaccg
     7141 tgtacaccgg gatcccggcc gccttggcga gatcggctcc ttcgagcggc tcgaggtcgc
     7201 cccgcgtctg cgcgccgtcc gagaggaaca ggatcgagac gagcccttgc gtctgcgtct
     7261 cgggcgctgc cgtcgtcgcc gaggcgaggt tgccgctcgg ccgccccaca gcttgctgcc
     7321 ctagcttgac cgcagccgcg agcgcgtccc cgatcgccgt gccaccgaag ctcgggaacc
     7381 attcgatcgt gtcgagtgac tgccggacga gatcgtggtt cgtcgtcggc ggtgaggcca
     7441 ctgcgggatc gccagcaaag gcgatcaggc cgacctgcag ccggtccggc acgcggtcga
     7501 ggaacgtgcg gacggccgcc gcggcggcgc cgatccgatt cggcttcacg tccttcgcct
     7561 ccatcgaacg cgacacgtcg atcacgagga tcacggtcgc gcggtcccgc gggacgagcg
     7621 tcgtgtgtcg cggacgagcc ataccgatac agagcgcagc gagcgcgagg aagaacaatg
     7681 ccagcggtat gaaccgtcgg cgataacggc cgcccgtcac gctcgccagc acgtcgagat
     7741 tcgtgaacct gatcgcgtaa cgcatccgcc tgcgctccgc cagcacgtag agcgccacag
     7801 ccagcggaac gacgaggagt gtcaggagaa ggagcggacg ttcgaacgtc acgagccacc
     7861 accgcaaacc gcgccgactt gcacgacagc ccgcacggct tgcaccgccg cagtcgaacg
     7921 aacgcgaact tccacgcgcc ggccgtgaac gctacgcgtg gcgctcacgc tctggatcag
     7981 ctcctgaggc ggcgccgact gcgtatagaa cgcgacggcg tgccaggccg agatcaagcg
     8041 ctcactcccg gcgcacgacg ccacggcgtg gacgaggccc ggacggaggc gcacggtgcg
     8101 gacccggttg gtcgtcggct cacccggcga gaaggccgca agggctctgc gcggcacggg
     8161 ccccgaccca ccgcccgccg acggcaggca accgagatgc gggcggaagc tcggcccttt
     8221 tggcgcgttc ccggtgtacg tgccgcggaa caatgccgaa cgtgccgtgg tcacgccggg
     8281 gttgacggga ctgccaagct tgccgaggaa gtcgatgtcg atcgcacggt cggtcagctc
     8341 cgcatccagc ccaccgatca cgtaaccgcg agggcacgac agctggaaat cgacgcgggg
     8401 tcgcggcgtg gtctgcgctg cgggcacgac gacccagggg ccggcgacac ggacgcacac
     8461 cggcagtccg cggcactcgt tcgtcgcgcc cgccgaggaa gcgctcattc ctgcgaccgt
     8521 agccaggaaa gctgccgcta ccaagagacg tttcacggca ccctcctgaa ccagagcgcc
     8581 gacaatgctc cgccgaacac caggaacgcc gcggagcctc cggcgaacag atcggtgatc
     8641 tcacgcttct ggcggcgatg accgagacgc gaaccgagct tctcgtagat ctgacgcaga
     8701 cgggtcgcat ccggcgccgt gaagaactgg ccggcggtca tacttgcgac cgttcgcagg
     8761 gtctccgggc taggcggcac ccgcagctgt gcctggtagc cgcccgcgac cggcacgttg
     8821 acgacaccgt ccggtgtacc gacgacgacc gtgtacaccg ggatgtgcgc cgacttcgcc
     8881 tgcgttgccg cgacctgcgg cgtcgtccgc ccgctcatct gagccccatc ggagatgacg
     8941 agcatcgcgg tcggggggat ccgcccgtcg ctggttcgct cggcccgcgc gagacgcgtc
     9001 ccgagcgcca ccgcgtcgcc gagcgccgtg ccttctcccg gtttgagcgc cttcagcgcg
     9061 ctgtgtacga gctcacggtc ttccgtcgga gggaccgcaa cataggcgcg gcccgcgaat
     9121 ccgatcacgc cgacgcgaaa cttctttggc atcccgtcga caaacgcctg cgccgcactc
     9181 tgcgcggcgg cgagcctcga cggccggacg tcctgcgcgc tcatcgagag cgacgagtcg
     9241 atcgcgatca acaccgtcgc ctcctcccgc tggacgctca cgctcgcgtg cggccgcgcg
     9301 acacccacga tcatcgcggc gagcgcgacg aggaagagag caacgggcag gtgccggcgc
     9361 cagccgggag ctgcggcgac gaggttgggc aacagagccg gcgcagtgaa gcgtgttgcg
     9421 tagcgctcgc ggcgccgttc gcggaggagg tacagcccga ccaacaccgg tacgagcacg
     9481 aggccgatca gtgcgagcgg agactgaaag ctcatcgacg tgcgtctcgg cgaaggaaga
     9541 cggcaaagga acggagccag tcgcccgcgg tcgtcagcac caggtgacgc gctcctgccg
     9601 ctgcgagcgt gcgagccagt tcggcccgct ctgccgcggc ggctgcagcg aagcgtgcgc
     9661 gcagctgctc gctgcgcgtg tcggcgcgaa gctgccttcc tgtctcggga tcgacgagcc
     9721 acagaacccc cgcgtttggc agctcctgct cgcgcgg
//
LOCUS       DASVEU010000053         8543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_427333,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000053 DASVEU010000000
VERSION     DASVEU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427333"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF225_02045"
     CDS             <1..1082
                     /locus_tag="VF225_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7144064.1"
                     /translation="IPVVVVATAVVGLLVGLPSRRLFGDYLAILTLFFGQAFVIFVNN
                     ANRISFPFFGHVDLTGGSNGIANIDPFNFFGFKIDSTEKQYYLLLIAVALVLILLMFV
                     NESRTGRAWRALREDPLAAEVMSIPVNRLKLLAFVFGAAVAGLTGAIYGTIATAALPG
                     DYDVGLLITIYAIMILGGFGSIMGIVVGAVVVSSVPELLRSSANARPLFYGAILVAIL
                     VKLRPWRLLAAEVAGLAAFGFAIHAIAAAAYPRLVHGESIGSGVFARALDHWMLFPKN
                     PVKIADGAYILLVAAIVMYPQLSRLWRAVLLPPLLYLVMFVWENLLLLHIGGATRLIL
                     LGALLVALMNVRPQGLFGTATVEIV"
     gene            1079..1876
                     /locus_tag="VF225_02050"
     CDS             1079..1876
                     /locus_tag="VF225_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144065.1"
                     /translation="MTDDATLLELKEVDKSFGGLRVIADLDLVVKEHEIVSVIGPNGA
                     GKTTLFNLITGIYRPDSGEIQLDDRNLVGVAPHKITELGIARTFQTLRLFLNMTVKEN
                     VMAAQYGHTKVGVLRSMVRTPAARREEKEIHRLAEEKLAFFGERLMGYRWDQFAYSLS
                     YANRRRLEIARAMATQARILLLDEPAAGMNPRETHEITELIGKLRDDGGYTILVIEHD
                     MHVVEGISDRVVALDHGVKIAEGSFEAVATDPRVVEAYLGLRKTETK"
     gene            1873..2601
                     /locus_tag="VF225_02055"
     CDS             1873..2601
                     /locus_tag="VF225_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144066.1"
                     /translation="MSTNGAPDLLRLTNIDTYYGEIHILENLNLNVREGELVSLLGGN
                     ASGKSTTLKTILGLVQPRNGSVEFRGEEVTGRNTSYRIRQGMAIVPENRRLFGPMTVL
                     ENLEMGAYLIRDTKGRKEDFERVYTLFPLLHERRSQLAGTLSGGEQQMVAMGRALMAR
                     PKLLLMDEPSMGLAPILVERSFEIIQQVHQSGVAMLVVEQNANMALSIADRGYVLSTG
                     RCVLSGPANELLQHEDLRKAYLGR"
     gene            2591..3745
                     /locus_tag="VF225_02060"
     CDS             2591..3745
                     /locus_tag="VF225_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7144067.1"
                     /translation="MAVEAATLAERCRHRFPIFEHQVYINSCSQGALSDSVRAAYGQY
                     LSDWDEKGAPWEYWVERQEAARGAFAGLVNAAPDEIAVTTSLSAGVSALASGLRFTNR
                     SKVVLTDWEFPTIGQIWHAQEARGARVSHVLAADDGTIPLEHFERAIDDDTLIVSITH
                     VCYRNGAMLDVPAVVELAHDRGALVLLDVFQAVGSLPIDVAELGVDFLGAGVLKYLLG
                     SAGLGFFYCRRDLVERVWPTQTGWFADENIFAMDHTDYSPARTAARFQSGTPPVPSIY
                     AGIAGIELMQEIGIADTREHVQALNAHLLDGLDELQATVVTPRAPERRGALICVKSTD
                     AKGLVVALGDEGIVTSERDENLRISAHCYNTVEDVDTVLAALERHRKLLA"
     gene            3829..6900
                     /locus_tag="VF225_02065"
     CDS             3829..6900
                     /locus_tag="VF225_02065"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX7144068.1"
                     /translation="MTEPAGPQLVPTSSSRSGATVVFGLTALIWTLWLGAIASAFSEH
                     ELYIFLWCGSAVALWFLLLGELQNPAGPALPVAFKWWVLTLGGPLGLAYLLYVRNNSA
                     PNRMSTQFAQATTSKPADLASRVAFLERRVSALQTIVDGLQAGRVTAPSRAAAPPPPS
                     PRPEPAPPPRPQPPVQLPPTAAPPTAPPLQPKVSTEFDWGRTLSAADLMGAKALAFAG
                     GIVTLLGVVFFFVLAVNRGWIGPGMRVAFGGVASGIVFGAGLWLQRRYESTYSALVAV
                     GTGIAGAYITLLAAVSLYDMVSKPVALVLAAVIASIAVAVSLVWEAEVVAGFGLIGAM
                     IVPATLVFQGGLREIGTAFVAVVFAGATIVTVRRRWWILLQVSALVSVPQALAQVAAA
                     DSPHTSIVSVAVAFWLLYLAAGLAFQFELGPALAAAPGSFLMGSAVFAGVSAALLYGQ
                     RDGGHQQGIALLAAAAVYLALAAALYRRVREAATLLGALGLALAGVGLAEALSGSLLT
                     YAWAAEAALLVWLSSRVRDARFQLPALVYLTLALVHSVVTEARPDHFLESMRHPASGA
                     PALLAIALAVLVFARVERSWPDGRPTRGILRALDPALDWLRANEAGVNAAMYALAGLA
                     TTYAASLGILELCQDVWPGNGIETPFEWGHVAIASAWALAGLAVVLGAVRRRSNAGLV
                     LGFGWLGVTVTKVVALDAATFAQTRYGISFLVVGAAVLAAGLVRELSVPGSVTGEGVS
                     ALFVSLVLLLAGALVLVPDRVGGADGNGLAVLGVGVLYTALAAVAFARPNQRDLTTFL
                     WIAGLALAAAGEEMLVGGVWLVLIYTLTAALLAVVATTVGERRLTVASLVYLTLGALL
                     ALGEEAPPTHLLVSRAHPAHGVPSLLFVIGATVVLAWSLSWSVRYRVQAIWVAGGLSV
                     YAASLLILEAMQRISNQDIQTDFQRGHTVVSAFWGLVALVSLYVGLKRKQGLLRVGGF
                     ILFAISLGKIFLYDLPSLSSVQRALSFLAVGAVLLLGGFFYQRLSAQFDER"
     gene            6920..7189
                     /locus_tag="VF225_02070"
     CDS             6920..7189
                     /locus_tag="VF225_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7144069.1"
                     /translation="MNKGALERLQERIRTADRGWFFAAVADKVAERRQERGLSQRELA
                     ELVGTTQSAIARLERGGRPPRIDTLLRIADALECELVVDLKPRRH"
     gene            7203..7517
                     /locus_tag="VF225_02075"
     CDS             7203..7517
                     /locus_tag="VF225_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7144070.1"
                     /translation="MPEGDIEVGTLQDASAAVARLLAVTHASYERRAQLEHALESRIS
                     IEQAKGIVAERYGLEVDEAFDLIRRAARSHRIKLYDLVGAIRPGEETPAELAAVIAAE
                     RS"
     gene            7514..7849
                     /locus_tag="VF225_02080"
     CDS             7514..7849
                     /locus_tag="VF225_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144071.1"
                     /translation="MSSREERIGLNEAVFREVNERIENLAETFDLNSQSLDLICECGD
                     AACVKRLTMTRAEYEKLRSDAHQFAVHPGHEYPDVESIVARRKGYDVVVKNQGAPERI
                     AEETDPRGT"
     gene            7857..8426
                     /locus_tag="VF225_02085"
     CDS             7857..8426
                     /locus_tag="VF225_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7144072.1"
                     /translation="MLVAALGDSITEGSPGYDSRRGGDETSQWEYWAARIEPRLEFRN
                     SGIYGQRTDEIMARLEGCIPGADLLVVQGGINDIAQGRSVDDVAANLRAMVRRGQELG
                     TRVVIVDVLPWNNGWPDAEPKIRRLNALIAELARAEGIPLLPFHDTLEDPDRPGRMHA
                     EWTSDGDHPSVEGYRRLGEVAARLADYSS"
BASE COUNT         1293 a         2867 c         2826 g         1557 t
ORIGIN      
        1 cgatcccggt ggtcgtggtc gccaccgcgg tggtggggct cctcgtcgga ctgccgtctc
       61 gccggctctt tggcgactac ctcgccatct tgaccctttt cttcggccag gcattcgtca
      121 tcttcgtcaa caacgccaac cggatcagtt ttcccttctt cgggcacgtc gatctgacgg
      181 gcggctcgaa cgggatcgcc aacatcgacc cgttcaattt cttcggcttc aagatcgact
      241 cgacggagaa gcagtactac ctcctgctga tcgcggtcgc gctcgtcctg atcctgctga
      301 tgttcgtgaa cgaatcgagg acggggcgcg cctggcgcgc cctccgcgag gatcccctcg
      361 ccgctgaggt gatgagcatc ccggtcaacc ggctcaagct gctcgcgttc gtcttcggtg
      421 ccgcggttgc cggcttgacc ggtgcgatct acggcacgat cgcgacagca gcccttcccg
      481 gcgactacga cgtcggactg ctgatcacga tctacgcgat catgatcctg ggcggcttcg
      541 ggagcatcat gggaatcgtc gtcggcgcgg tggtcgtcag cagcgtcccc gagcttctcc
      601 gctcgtcggc caacgcccgc ccgctcttct acggcgcgat cctggtcgcg attctcgtca
      661 agctgcgccc gtggcggctg ctcgcggccg aggtcgccgg actggccgca ttcggtttcg
      721 cgatccacgc gatcgcggcg gcggcatacc cacgcctggt gcacggtgag tcgatcggct
      781 cgggtgtctt cgcgcgcgcc ctcgaccact ggatgctgtt cccgaagaat cccgtgaaga
      841 tcgcggacgg cgcgtacatc ttgctcgtgg cggcaatcgt catgtacccg cagctcagtc
      901 gcctctggcg agccgtgctc cttccaccgc tcttatatct ggtcatgttc gtgtgggaga
      961 acctgttgct cctgcacatc ggcggagcca ccaggctgat cctgctcggc gcactgctcg
     1021 ttgcgctcat gaacgtccgt ccgcaggggc tcttcggcac cgcgaccgtg gagatcgtgt
     1081 gacggacgac gcgacgctgc tcgagttgaa ggaagtggac aagtccttcg gcgggctgcg
     1141 cgtgatcgca gacctcgacc tcgtcgtcaa ggagcacgag atcgtgagcg tgatcggccc
     1201 gaacggcgcc ggcaagacga cgctcttcaa cctgatcacg ggcatctatc gccctgactc
     1261 aggcgagatt cagctcgacg acagaaatct cgtcggcgtc gcgccgcaca agatcaccga
     1321 gctcggaatc gctcgcacct tccagaccct gcgcctcttt ctgaacatga cggtgaagga
     1381 gaacgtcatg gcggcgcagt acggtcacac gaaggtcggc gtgctccggt cgatggtccg
     1441 cacgccggcg gcgcggcggg aggagaaaga aatccatcgc ctcgcggagg agaagctcgc
     1501 cttcttcggg gagcgactga tgggctatcg ctgggatcag ttcgcgtaca gcctgtcgta
     1561 cgcgaaccga aggcggctcg agatcgcgcg cgcgatggcg acgcaggcgc gcatcctgct
     1621 cctcgacgag cctgccgccg ggatgaaccc gcgcgagacc cacgagatca cggagctgat
     1681 cgggaagctc cgcgacgacg gcggctacac gatcctcgtc atcgagcacg acatgcacgt
     1741 cgtcgaaggc atctcggacc gcgtcgtcgc cctcgatcac ggagtcaaga tcgccgaggg
     1801 atcgttcgag gcggttgcga ccgatccgcg agtcgtcgag gcgtatctcg gcctgcggaa
     1861 gacggagacg aagtgagcac caacggcgca ccggatctgc tgcggttgac gaacatcgac
     1921 acgtactacg gggagatcca catcctcgag aacttgaatc tgaatgtccg cgaaggcgag
     1981 ctcgtgtcgc tcctcggcgg caacgcctcc ggaaagtcca cgacgctgaa gacgatcctt
     2041 ggcctcgtgc agccgcgcaa cggctccgtt gagtttcggg gcgaggaagt gaccggccgc
     2101 aatacgagct accggatccg ccaaggcatg gcgatcgttc ccgaaaaccg ccggctcttc
     2161 gggccgatga cggtgctgga gaacctcgag atgggcgcgt acctcatccg ggacacgaaa
     2221 gggcgcaagg aggacttcga acgggtctac acgctcttcc cactcctgca cgaacgacgc
     2281 agccagctcg ccggcacgct gtccgggggc gagcagcaga tggttgcgat gggacgcgcg
     2341 ctgatggcgc gcccgaagct gttgttgatg gacgagccgt cgatgggtct cgcgccgatc
     2401 ctggtcgaac ggagcttcga gatcatccag caggtgcatc aatccggcgt cgcgatgctc
     2461 gtcgtcgagc agaatgcgaa catggcactc tcgatcgccg atcgtggcta tgtcctctcg
     2521 acggggcgct gcgtcctcag cggaccggcg aacgagcttc tccagcacga ggacctgcga
     2581 aaggcctatc ttggccgttg aggcagcaac gctcgccgag cgctgccgcc accgcttccc
     2641 gatcttcgag caccaggtgt acatcaactc ctgctcgcag ggagcgcttt ccgactccgt
     2701 gcgcgccgcc tacgggcagt acctctccga ctgggacgag aagggcgctc cgtgggagta
     2761 ctgggtcgag cggcaggaag cggcacgcgg cgccttcgcg ggtctcgtca acgcggcccc
     2821 ggacgagatc gccgtgacga catcgctctc tgcgggcgtg agcgcgctcg cgagcggcct
     2881 gcgctttacg aaccggtcga aggtcgtcct cacggactgg gagttcccga cgatcggcca
     2941 gatctggcac gcgcaggagg cgcggggcgc acgcgtctcg cacgttctcg cagcagacga
     3001 cggcacgatc ccgctcgagc acttcgagcg tgcgatcgac gacgacacgc tcatcgtctc
     3061 gatcacgcat gtctgttacc gcaacggcgc gatgctggac gtccctgcgg tcgtcgagct
     3121 tgctcatgat cgcggcgcgc tcgttctgct cgacgtgttc caggcggtcg gctcgctgcc
     3181 gatcgacgtt gccgagctcg gcgtcgactt tctcggcgca ggggtcctca agtacctgct
     3241 cggttcggcg gggctcgggt tcttctattg ccggcgcgat ctggtcgagc gcgtctggcc
     3301 gacacagacc gggtggttcg cggacgagaa catcttcgcc atggatcaca ccgactactc
     3361 gcccgcgcgc acggctgcgc gcttccaatc gggaacgccg ccggtgccgt ccatctacgc
     3421 gggcatcgcc gggatcgagc tgatgcagga gatcggcatc gccgacacgc gcgaacacgt
     3481 ccaggctctg aatgcgcatc ttctcgacgg tctcgacgag ctgcaggcga ccgtcgtcac
     3541 accgcgggcg ccggaacggc gcggcgcgct catctgcgtc aagtccaccg acgccaaggg
     3601 cctcgtagtt gccctcggcg acgaaggcat cgttacctcg gagcgcgacg aaaacctgcg
     3661 catctcggct cactgctaca acacagtcga ggacgtcgac acggtcctcg ccgcactcga
     3721 gcgccaccgc aagctcctgg cctaggacac ttgcaaccgg ggttgacccg gcatagcaaa
     3781 aatgctatac aaggtggcaa cggatgctag aggcgcaagg agcccgcgat gactgagccc
     3841 gccggcccgc aactcgttcc gacgtcgagc tcccgatcgg gggcgaccgt cgtcttcggt
     3901 ttgaccgcac tcatctggac gctctggctc ggcgcgatcg cgtccgcctt cagcgagcat
     3961 gagctctaca tcttcctctg gtgcgggagc gccgttgcgc tctggttcct actcctcggg
     4021 gagctgcaga atcccgcggg gcccgcactt cccgtcgcct tcaaatggtg ggtgctcacc
     4081 ctaggcggcc cgctcggcct cgcctacttg ctctacgttc gcaacaactc ggctccgaac
     4141 cggatgtcga ctcagttcgc gcaagctacg acgagcaagc cggccgatct tgccagccgt
     4201 gtcgcgttcc tcgagagacg cgtctcggcg ctgcagacga tcgtcgacgg gcttcaggcc
     4261 ggccgggtca cggctccgag ccgcgcggca gcgccgccgc cgccgtcacc gcgcccagag
     4321 cccgcacctc ctcctcggcc acagccaccg gtgcaactac ctccaactgc cgccccgcct
     4381 acagctccgc cgctacagcc gaaggtcagc acagagttcg actggggtcg cacgctgtcc
     4441 gccgccgacc tgatgggagc gaaggcattg gcgtttgcgg ggggcatcgt cacgctgctc
     4501 ggcgttgtct tcttcttcgt cctcgcggtc aaccgcgggt ggatcggacc tgggatgcgc
     4561 gttgccttcg gcggtgtcgc ctcggggatc gtgttcggcg ccggcctttg gctgcagcgg
     4621 cggtacgagt cgacgtactc ggcgctcgtg gcggtgggca cgggcatcgc aggcgcctac
     4681 atcacgttgc tcgctgcggt ctcgctgtac gacatggtct cgaagccggt cgcgcttgtg
     4741 cttgccgcgg tgatcgcgtc gatcgcggtc gcggtctcgc tcgtgtggga agcggaggtc
     4801 gtcgcaggct tcggcttgat cggggcgatg atcgtgccgg cgacgctcgt cttccagggc
     4861 gggttgcggg agatcggcac tgcgttcgtc gcggttgtct ttgcgggagc cacgatcgtt
     4921 actgtccgcc ggcgctggtg gatcctgctg caggtttcgg ccctggtcag cgtgccccaa
     4981 gcgcttgcgc aggtggcggc cgccgattct ccgcatacga gcatcgtctc ggttgccgtc
     5041 gcgttctggc tcctctacct cgccgcgggt ttggcgttcc agttcgagct cggccctgcg
     5101 ctcgctgccg cgcccggatc gttcttgatg ggcagcgcgg tctttgcagg cgtgtccgcg
     5161 gcgcttctct atggccaacg cgacggaggt caccagcagg gcatcgccct gctcgccgcc
     5221 gccgccgttt accttgcgct cgccgctgcg ctgtaccggc gcgtccgcga ggccgcgacg
     5281 ctgctcgggg cgctcggtct tgcgcttgca ggggtcggcc tggcggaagc gttgtccggg
     5341 tcgttgctga cctatgcctg ggccgccgag gctgctctgc tcgtctggtt gtcgagccgt
     5401 gttcgcgacg cccgcttcca gttgccggct cttgtctatc tgacgctggc gctggtccat
     5461 tcggtcgtca ccgaagcgag gccagaccat ttcctcgagt cgatgcgtca ccccgcgagc
     5521 ggtgcgcctg cgctgctcgc gatcgcgctg gcagtgctcg tcttcgcgcg tgtcgagcgt
     5581 tcgtggccgg acggccggcc gactaggggg atccttcggg cgctcgaccc tgcactcgac
     5641 tggctgcgtg cgaacgaagc cggcgtgaac gctgcgatgt atgcactggc tggactcgcg
     5701 accacgtacg cagcgtcgct ggggatcctc gagttgtgcc aggacgtctg gcccggaaac
     5761 gggatcgaga caccgttcga gtggggccac gttgcgatcg cctcggcctg ggcactcgcg
     5821 ggtcttgccg tcgtcctcgg cgccgtgcgg cggcgctcca atgccgggct cgtgttgggg
     5881 ttcggctggc tcggcgtcac cgtcaccaag gtcgtggcac tcgacgcggc cacgttcgct
     5941 cagacgcgtt acggcatctc gttcctcgtc gtcggcgccg cagtgctcgc ggccggcctc
     6001 gtccgcgagc tttcggtgcc cggctccgtg acgggtgaag gtgtgagcgc gctcttcgtc
     6061 agcctcgttc tgctccttgc cggcgcgctc gtcctcgttc cggatcgcgt gggtggagcc
     6121 gacggcaacg gtctcgctgt cctcggagtc ggggtgctct acacagcgct cgcggccgtc
     6181 gcgttcgcgc gcccgaacca gcgcgacctg acgacgttcc tgtggatagc cggtctggcg
     6241 ctcgcagccg caggcgaaga gatgctggtc ggcggcgtgt ggctcgttct gatctacacg
     6301 ctcacggccg ccctgctcgc cgtcgtcgca actaccgtcg gcgagcggcg cctaactgtc
     6361 gcgtcgctcg tctacctcac cctcggtgca ctgctggcgc tgggggaaga agctccgccc
     6421 acacaccttc tggtctcgcg cgctcacccg gcacacggcg taccgagcct gcttttcgtc
     6481 atcggcgcga cggtggtgct tgcctggtct ctctcgtgga gcgtgcgcta tcgcgtccag
     6541 gcgatctggg tcgcgggcgg tctttcggtc tacgccgcct cgctcctgat cctggaagcg
     6601 atgcagcgca tctcgaacca ggacatccaa acggactttc agcgtgggca cacggtcgtg
     6661 agtgcattct gggggctggt cgccctcgtc tcgctctacg tggggctcaa gcgaaaacag
     6721 ggtctgctgc gggtcggcgg gttcatcctc ttcgccatca gcctcggcaa gatcttcctc
     6781 tacgacctcc cgagcctgag ctctgtgcag cgtgcgctct cgttcctcgc ggtcggcgca
     6841 gtcctgctcc tcggcgggtt cttctaccag cgcctgagcg cccagttcga cgagcgatag
     6901 catttctgca atgccgcgca tgaacaaggg cgccctcgag cgtctgcagg agcgaattcg
     6961 caccgccgac cgcggctggt tcttcgcggc cgtggcggac aaggtcgccg agcggcggca
     7021 ggagagagga ctctcgcagc gcgagctggc cgagctcgtc ggcacgacgc agtcggcgat
     7081 cgcgcggctg gagcggggag gccgtccgcc gcggatcgac acgttgctcc gaatcgccga
     7141 tgcgctcgag tgcgagctgg tcgtcgatct gaagccgcgc cggcactaga ctcctcagtc
     7201 tcttgccgga aggtgacatc gaggtgggca cgctccagga tgcgtccgct gccgtcgctc
     7261 gcctgcttgc cgtcacccat gcctcctacg agcgccgggc ccagctcgaa cacgccctcg
     7321 agtctcggat ctccatcgag caggcgaagg gaatcgtcgc ggaacggtac ggcctcgagg
     7381 tcgacgaggc cttcgacctg atccgccgag cggcgcgctc gcaccggatc aagctttacg
     7441 acctggtcgg agcgatccgc ccgggtgagg agacaccggc tgagctggca gccgtgatcg
     7501 ccgccgaacg gtcatgagct cacgcgagga gcgcatcgga ctgaacgagg cggtcttccg
     7561 ggaggtcaac gagcggatcg agaatctcgc ggagacgttc gacctcaact cgcagtcgct
     7621 cgatctcatc tgcgagtgcg gcgacgccgc gtgcgtgaag cgtctcacga tgacaagagc
     7681 cgaatacgag aagctccgct ccgacgcaca tcagttcgcc gtccatcccg gtcacgagta
     7741 tccggacgtc gaaagcatcg tcgcgaggcg gaagggttac gacgtcgtgg tgaaaaacca
     7801 gggcgcgccc gagcggatcg cagaggagac cgatccacgc ggaacctgag gaacgaattc
     7861 tcgtcgctgc gctcggcgac tcgattaccg agggctcgcc cggctacgac tcacgcaggg
     7921 gcggggacga gacgagccag tgggaatact gggcggctcg aatcgagccc cggctcgagt
     7981 ttcgcaacag cgggatctat ggccagcgga cggacgagat catggcccgg ctcgagggct
     8041 gcatccccgg cgccgatctg ctcgtcgtcc agggcggcat caacgacatc gcgcaagggc
     8101 ggtcggtcga tgacgtggcc gccaacctgc gcgcgatggt gcgtcgcggc caggagctcg
     8161 ggacgcgggt cgtcatcgtc gatgtgctgc cgtggaacaa cggctggccc gacgccgagc
     8221 cgaagatccg gcggctcaac gccttgatcg ccgagctcgc gcgcgccgag ggcattccgc
     8281 tgcttccgtt ccacgacacg ctcgaggatc ccgatcgccc gggacgcatg cacgccgagt
     8341 ggacctcgga cggtgaccat ccgtcagtcg agggctaccg taggctcggc gaagtcgccg
     8401 ctcggctggc ggactacagc tcgtagacct cgccgtcggc tgcgcgctcg acgccgtcgg
     8461 gcagagaaag ctcttcgggg cgatggatga tcacgagccg gcgtgccccg gattcctcga
     8521 aggcggcgat tgcctcctcc gcc
//
LOCUS       DASVEU010000054        23984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_232936,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000054 DASVEU010000000
VERSION     DASVEU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 23984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23984
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232936"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF225_02090"
     CDS             <1..618
                     /locus_tag="VF225_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144073.1"
                     /translation="ATRRYRRALETTGVAAAAIMIPWAVLGFRDFAEYPRVLRLLVRN
                     ELPTSFSTKALAGMLGFGPAALALQLGSVVAVFALARRHEGDRRAFSAAIFAALALSP
                     LVWIHYYVLLIVPIGLAARRLNAVWLLPILAFWVYTDNLSSVWPVMWVSLVVMGVAVL
                     TLRPARSEPRGSLRVEEYRPASTSSRRIGRRSAEPSLRATTAARP"
     gene            691..1056
                     /locus_tag="VF225_02095"
     CDS             691..1056
                     /locus_tag="VF225_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144074.1"
                     /translation="MPNQLDFALIKRLREVLDRRPATETELRTLKEQAEGWQRAVSGQ
                     LESSERRLLRLNANPASSLAQIASELRRVEKLRPQLDEVRGLLADLENRARELRTEWL
                     LSQATSAKASSRRPDGRQP"
     gene            complement(1025..1369)
                     /locus_tag="VF225_02100"
     CDS             complement(1025..1369)
                     /locus_tag="VF225_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7144075.1"
                     /translation="MAEVAIRREDGSIVCERCVLGDTALTRMKGLLGRRELPSGEGIL
                     LKPASSVHMAFMRFPIDAVFLDRDLRVVKIASDLRPWRVAGSRGAKAVLEIPAGEADR
                     RGLTVGDRLAVD"
     gene            complement(1369..2724)
                     /locus_tag="VF225_02105"
     CDS             complement(1369..2724)
                     /locus_tag="VF225_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144076.1"
                     /translation="MPRRFIRRDNWEFGGQPTPAEGTSGEPKSRKRRLATTLVFTTLF
                     FAGASLAAVAGNQFGPLPGEDNQQLAGDTTTTDAGTPPVATDDTSAAVAAPVDAGAGP
                     ADASTDATATTDATATATPTDTTSDATPVDDTTPPASLTGPSTAGTAAIPTATAAKQS
                     AKQTAKAARGWKATKLRTILLPKLKPAPPPEIEGPPSAATIWLNSQLPDPTPPALRLS
                     PKFASNLKATAKASGLNWATMLGILRARGATGHNPANRPTLRKLAGRLSSYGPAKGDW
                     ARIVSYSGDTSFADRAAALARYDRAVGLKGLVKGLEAAKKSIATRLLSDPNVSIYEGG
                     RNDIVQNKVDVRVLAVIAYLRESFGQVTVSSLVSGHRLYARPGVISAHISGHALDVSG
                     LGDTPIQGHQEPGGITERAVRDILFLPTEVMPRQVISLLGLGGASFPMADHYNHIHIG
                     F"
     gene            3074..3250
                     /locus_tag="VF225_02110"
     CDS             3074..3250
                     /locus_tag="VF225_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144077.1"
                     /translation="MSFKDLVHYLRARFAVEEGQTMAEYGVVLAVIAVAVVVALGLLS
                     TSISAAFDAVRTKL"
     gene            3284..3469
                     /locus_tag="VF225_02115"
     CDS             3284..3469
                     /locus_tag="VF225_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144078.1"
                     /translation="MKHHRRHRIREFLTFLSREHAQAEPDKTVTLAVVSGSSFLAFTR
                     FSENGASAVTSLVRLFT"
     gene            3541..3939
                     /locus_tag="VF225_02120"
     CDS             3541..3939
                     /locus_tag="VF225_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7144079.1"
                     /translation="MHLKDTIKNERGQTMTEFAFVLPILLVVLFGIIQFGIIFNNYVA
                     LTDAARAASRQGAVSRNAADPEGACEATGYSAGANLDQPHVKFILTCDSSWAAGSDVT
                     VTATYPYDIKLLDWVVASGTLNTTMKERVE"
     gene            3936..4865
                     /locus_tag="VF225_02125"
     CDS             3936..4865
                     /locus_tag="VF225_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7144080.1"
                     /translation="MSVRKSESGQAFVLMALSLVVIMGMAALVVDVGNWYHTKRRLQA
                     TADAAALAGAQLLPKDSAGAQAMALSYANKNGGDVAGADIAVTSTAVADDTIGVRARK
                     TDAGVFGSVLGIASADISARAKARVGPPLKARYVAPMVVFCDHPLIHNCNGDSTPDFG
                     SDTTMDYDPMGAPGAFGMLNLDGGNGTPGSSDEADWILHGFNKYLGLGDYRSDPGAKF
                     SSSNIQDALEARRGSVLLFPVYRQLDGQGQNAAYEIIGWIGFHLTSYEVHGNNSTLHG
                     YFTEFIAQGILSEGGGDGGPSSSFGVKSIQLIE"
     gene            4906..5676
                     /gene="cpaB"
                     /locus_tag="VF225_02130"
     CDS             4906..5676
                     /gene="cpaB"
                     /locus_tag="VF225_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX7144081.1"
                     /translation="MNYRAKNIGIAVALAALAAILTSVYVVNYKRHVQNGEGKVTVLV
                     AARDIPAGTSGADVVDQKMLKEQTVPRKAVVAGAISSPDQLAQYVATQDVFEGEQVST
                     RRFSPPTEQGIRAQIKGTQRAYQITGDAHQLLAGTIQAGDHVDVVGTWGTNSGQGANA
                     STNSGGPVARVVLRNLLVLRAPGGVGATAKLVEGSGESVQLRVTDSQSQKLIWIEKNG
                     KEGGWHLELRPPSNSLDSSNTVQDSRTMLFDGPGRRPR"
     gene            5673..6863
                     /locus_tag="VF225_02135"
     CDS             5673..6863
                     /locus_tag="VF225_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7144082.1"
                     /translation="MSSATETTQRVRVYATGSCEGFDKLRESLASHPEIELVGASTNV
                     ADGAAALAGGHLDAVLHATRAASLPADELAAIREHSRAPILMLASGGSSGLLEQALDA
                     DVADVLLLPQLVENIVFAIRKATHTTRRHHDTGSGRHGKIVTVFSPKGGTGKTVTATN
                     LAAACAKFEGRKTLLLDLDLQFGDAAIMLGIEPDKTIYDLVVAPGELDTEKLAGYTTK
                     HACGLEVLPAPLRPEDAELVTEAKLGRLLEVARESFDVIVVDTSPFFHGPMLATLDRT
                     DELLLLCSLDVPTLKNLRLALQTLDLLSFPKQRVRIVLNRANSKVGMKPNEVEGALGM
                     KVRFELPSDRAVPLSVNRGNPVVLAEEGADVSKAIKAMAKELFPAVKEHGKKRRLLPA
                     LARS"
     gene            6867..8315
                     /locus_tag="VF225_02140"
     CDS             6867..8315
                     /locus_tag="VF225_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX7144083.1"
                     /translation="MGLHDRLKTSNGGSSATAELLTGGHSHETSSPSPTQDPYAELKT
                     RIHHACIATLGAELFKREATGDMGERVTRAVTEQLVLDRTPLTREERRQIIREITDDI
                     LGYGPLETFLRDDSVTEVMVNAYDQIYVERLGKLERVQASFADNSHLLRIIDKIVSQI
                     GRRVDEASPMVDARLPDGSRVNAIIPPLALKGPTLTIRKFARDPYTMNDLISFGSISP
                     KAAQFLAACVKGKLNVLISGGTGTGKTTTLNAMSAFIPGDERIVTIEDAAELQLQQEH
                     VITLESRPSNIEGQGEVRIRELVRNALRMRPDRIIVGEVRGPETLDMLQAMNTGHEGS
                     LTTIHANSPRDALSRLETLVLTAGVELPLRAIREQVSSAFDLLVQITRLVDGSRRISH
                     VTEVLRMESEVITLQDVFHAKPPDEQTAASGVGLRLLSPLSCTGLKPNFLEKMAANGV
                     VLQPSFFSREDDTDAPAVGQGHEASKFKGAFQ"
     gene            8312..10180
                     /locus_tag="VF225_02145"
     CDS             8312..10180
                     /locus_tag="VF225_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7144084.1"
                     /translation="MSRFRLAVVGLAFILLAVPAAAGAAVQLRGVDATSYPTIRASIY
                     TPTGSGRPALWENGTPVAGFQAQNLGSVKAIATLIDRSQSMRGKPLADAAAGARAFVA
                     AKQPADELSVIAFGSKVATLSPFSTVKLDADLPLARMQTDRVRGTALYDAVIAAANQL
                     KGNPLPGRVIIVLSDGADNASTGTLATAIKTAAEANASVYAIGIEGAGFTSDPLVQLA
                     SATGGQYYGASSTSELARVYASIAAALKKTWRVQYVTAARPGDSVRLTASIVGAGSTT
                     QLAKIPAGDAIAAPQPTNLLPKNAYGPGGPLAIAIAVAALVLFGCLFFLAGIRGSWVR
                     SRIVAHTGEGKVNAKKNRKERRNEAFSSIFAATEKALGDLKHWRAISRMLERADVQLR
                     TVEFVWITVGAAVLMALLALLFGASPVLLLALTVTAGMIPFSWVWLKMRKRLKAFEDE
                     LPDLLITIAASLKAGHSFKQGLQAVVDEGHPPANQEFKRVLTEASLGRPMDDALLEMA
                     ERMASKNFEFAITAVTIQRQVGGSLATLFDMVADTVRQRQQFTRKIRALTAMGRMSAY
                     TLIGIPFFLAGVITLVNRNYMHPLYFSHTGHIVMIVGLVMMGIGSLILKKIVSFRG"
     gene            10185..11093
                     /locus_tag="VF225_02150"
     CDS             10185..11093
                     /locus_tag="VF225_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7144085.1"
                     /translation="MSMILTLAIAAIGGAVFFFAELATTPMRNRRNLVHRAANYGRLR
                     TVTGKEMPRFRERALDPFVAKAARLVLRINPRMSVEAVSEKLMAAGMRRTSPTTIIGA
                     KGILGLGGVILGLALGSASAPKYMFLIALFFGLSGALAPGFYLNSRMKSRQLAVSAEL
                     PDALDLLSVSVEAGLGFDGAVQKLTEHMDGPLIEEFELALGGIRIGEGRSEALKKMSE
                     RSSSQEMASFVRAIIQADQLGISLGRILKVQAGDTRLKRQLIAEEKAMKAPIKMLFPT
                     VIFIFPAMFLVVLGPAFLNLSALFKF"
     gene            11110..12576
                     /locus_tag="VF225_02155"
     CDS             11110..12576
                     /locus_tag="VF225_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144086.1"
                     /translation="MAEHGTEGGFGTGLRAKLQSGETPPEPRSPAEAIAAASPLVEET
                     EAEALRAELNASLAREQQLRSSLHDQVDTAARDEQLEHQLAEQSAGLDRRAAALAETQ
                     AELDERERRMTERLAELDGLLEAREELTKLEARLAEREQLVELKVQELKVGDGERAAT
                     AAEVEEKLAEITKREQDLAKAEARVSAAGEETETRQAKLMRALEEREEKARVKDEQAR
                     ALEKRVAEREAQLAATEREFTQARVQLETREKVIGKREAAISAESGRDVEQLSARARE
                     LDERELELSTRANDLTARMEDLERQAAERDAVAKDALAKLDSIEQRETELIAREAELH
                     RLEATARRAAEEARKPEVDELAERRLKQRDDELSARESALNKAEQAILASRSRYEGKE
                     KGLDEREKVLAGRARELEERDAALNLREAQMEAELEVREQRLEQREKAGAELEAALST
                     RESDLSMYVSQLQDNFDRRESEWWSKQLGKDAGQQPAA"
     gene            12606..13169
                     /locus_tag="VF225_02160"
     CDS             12606..13169
                     /locus_tag="VF225_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144087.1"
                     /translation="MAQTLSDSELVARCRSGDQQAWAELVDRFSRYVYAISVQAFRLS
                     DADAEDVFQEVFARAYEHLDGLRDDAAVRPWLAQLTRRLCIDRLRATSRERPATDEDL
                     ELAGSEETLTMLEDALTVHEALAATPEHCREILDRFFARDESYRTIGEALDLPAGTIA
                     SRISRCLGRLREFLEGRTQPDPPSSSR"
     gene            13166..13420
                     /locus_tag="VF225_02165"
     CDS             13166..13420
                     /locus_tag="VF225_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144088.1"
                     /translation="MTAFDEERLARLIRALPPAPEAWVRAAQELPLAKAGLDEIVARA
                     EADAHFKARLVADLESALEAEGYERDPAVIKALKVRFKSK"
     gene            13442..14023
                     /locus_tag="VF225_02170"
     CDS             13442..14023
                     /locus_tag="VF225_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX7144089.1"
                     /translation="MELLDLRLREFLDEIAGEGRTPGGGSVAALVTAMAAGLLAKVAR
                     RSVDTWPDAAGIAAQAESLQARAAPLAQADAEQYEAALLARGQSGEGQGARQDFALGQ
                     AYAKAAEPPLQIARAAGDVAQLALTVAQSGDAALWADAVTAALLAAAAAKAAAELVAV
                     NLTASAGDRRVLEAAKVAEEAARAAEAARAVRE"
     gene            14287..15105
                     /locus_tag="VF225_02175"
     CDS             14287..15105
                     /locus_tag="VF225_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144090.1"
                     /translation="MSEELPAWSLSAEAVESIALPEEWPGQVTREWAWGGATGAGARV
                     CVLDSGIETDHPLVGDVQKSVAISIEGDEVIIEDNDTAGDLCGHGTACAGVIRSLAPD
                     CELVSVRVLGAGYTGSGAVLLEGLRWAIAQGYDVINMSLSTTKQKFASLLHELADTAY
                     FRRTMLVASAHNMPVESYPWRFSSVISVGSHEQEDPYQYYYNPAPPVEFFARGVDVEV
                     AWNGGGRLVCTGNSFATPHIAGIAALVLSKHPELTPFQLKSVLYLTATNVGGEK"
     gene            15102..15731
                     /locus_tag="VF225_02180"
     CDS             15102..15731
                     /locus_tag="VF225_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7144091.1"
                     /translation="MSDELRAAVAAGVLGSEDQYRALLQSIVDVARAIFRAKASSILL
                     LDEEADELVFEAAADEASEALIGKRFPSSTGIAGFVLVSRQPLVIEDVLTDPRFSRET
                     AESTGFVPKGLMAVPLLHEERVLGVLEVLDRPQDTRFTLAEMELLGLFASQAAIALDL
                     LQRARRAQEALAGDGELSVISRLAEKLEARREEGSETALRLLAELEHLL"
     gene            complement(15784..16020)
                     /locus_tag="VF225_02185"
     CDS             complement(15784..16020)
                     /locus_tag="VF225_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144092.1"
                     /translation="MSDDRFEQEEAKDEDVEAHKHKGFKANEELLANQELLATGEPES
                     DDVEAHRHKGFKANEEPGSDDSDDVEAHRHKGFK"
     gene            16140..17810
                     /locus_tag="VF225_02190"
     CDS             16140..17810
                     /locus_tag="VF225_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144093.1"
                     /translation="MRQILGHLAESSRAFRDVFRNPGLRRIELAWAASILGTWAYGIA
                     VVVYAYEQGGATAVGVVGLARWVAAAIASPFAAILGDRYDRRWVMAGSDIARAVLIAG
                     AALAVFAGAPPIVIYVIAGGVSVVATAFRPAEAALIPSLARTPEELTAANVAASTIES
                     VGIFGGPAIGGLLLAAAGTGVVFLVTGGAMLWSAFLIARIRPGIEVEDSGEREPVSVL
                     DELLAGFRAIAHERRTRFLVGLFSAQTFVDGMLSVLIVVIALKLLDTGQAGVGFLNSA
                     VGVGGLLGALAAAALVGRRRQAADFGLGIFIWGVPIALVAIWPNQAFVLVLLAVVGIG
                     NTIVDVSGMTLLQRSAPDDVLARVFGVLESVLLLAGALGAVAAPLLLNWLGTRGALIV
                     AGSLLPLVVIPAWPRLNAIDRAAEVPVERLEFLRANPIFAPLPASTLEQLAHALEEVP
                     ASVGEEIVRQGEPGDRFYLIKHGGFDVYIDGEPVQSLDTGDSFGEIALLRNVPRTATV
                     RARTEAVLYALDRRHFLAAVTGFGPSLSAAEGIIGMRQGTGRAGIVRA"
     gene            complement(17761..18774)
                     /locus_tag="VF225_02195"
     CDS             complement(17761..18774)
                     /locus_tag="VF225_02195"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX7144094.1"
                     /translation="MAEVTGLVLPPPLVPGGTIGVAAASSPFEERSEVDRGVRWYEER
                     GYRVKLAEHVYARDDFVAGDAEARARDVNRLFADPEVDIVQLLRGGYGASQVVPHLDF
                     DLIAENPKPLVGYSDVTALHVAIRQRTGLVTFYGPGFTGMGSPKRGDWSKERFLRALT
                     DSEPLGELPPRPDDPYIGSIGTGKATAPLVGGCLWLLRETLATPWEVDFDDAILFFED
                     VHCPPWHVDGMLTQLRNAGKLDRIAGVVIGEMYKCEEHLQPEPWLRSRSMEDVFEHHL
                     EPLGVPILHNLPLGHGDHLCTIPLGVTATVDADARRLTFAEPALETRPARSRRDPFPA
                     ASR"
     gene            complement(18829..20265)
                     /locus_tag="VF225_02200"
     CDS             complement(18829..20265)
                     /locus_tag="VF225_02200"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX7144095.1"
                     /translation="MSTTVSKHQNFVGGEWVDAAGGETMEVLNPSTGETIAEVPRASA
                     EDVDRAVQAAKKALPEWLETTPGERAEMLLKLADAIDEHTEELAELESQNVGKPLAAA
                     RDELPVCSDNIRFFAGAARVLEGKSAGEYMRGYTSWLRREPIGVVGGIAPWNYPLMMA
                     VWKVAPALAAGNTQVLKPSEQTPLTTLRFAQLAADILPAGVLNVITGDGVPAGEAIVK
                     HPDVRLVSLTGDVETGKIIARTAADNLKRVHLELGGKAPVIVFDDADPAAVAEGIKIG
                     GYWNSGQDCTAASRVVAGPKIYDKLLEELVPAVESLHVGDPAEGDEIEMGPVISKDQQ
                     ERVLGFLDRAKGATVLTGGGTNGDRGFFIKPTVVVDVGQEDEIIQREVFGPVVTVQRF
                     ADDDQAIAWANDVNYGLAASVWTRDIGRALNASRKLQFGTVWINDHIPLVSEMPHGGF
                     KQSGYGKDLSAYSLEDYTQIKHVMAKLD"
     gene            complement(20269..21075)
                     /locus_tag="VF225_02205"
     CDS             complement(20269..21075)
                     /locus_tag="VF225_02205"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144096.1"
                     /translation="METRATRLSLSVWTVLIVLFLWIPLVLICVYAFNSSNIQSWPIA
                     GFSTKWFHLAWHNEEARSALVLSLKAAGIATSIALVLGTAAATVVSRFRFFGRDAITF
                     LLVLPIALPGVVTGIALNSFFNNNPIHGLTPSLWTIVIGHSTFCIVVVYNNVVARLRR
                     TQTSLTEASMDLGADGWQTFRFVTFPVISTALVAGALLAFALSFDEVIVTTFTAGAQN
                     TLPIWILGNLRLGQQLPQVNVVVFFVIVMTVVPVVIAQRLTRDTGILRRR"
     gene            complement(21075..21902)
                     /locus_tag="VF225_02210"
     CDS             complement(21075..21902)
                     /locus_tag="VF225_02210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144097.1"
                     /translation="MRAVALLLPPLGWMLVFYLAALAVLFVSAFWSVDSFTGKLTHVW
                     TWTNFDTIIHDPTYRQIAWRTIWMAALVTLTDALIAFPFAYYMARVATPRLRTLLFVL
                     VLLPLFSSYLARIYAWRLILNQDGALNWSLAKIGLPQQDWAFTSTAMWIVFSYIWLPF
                     MILPIYAALERIPDSYLEASRDLGARNWTTFRRVIVPLALPGIAAGSIFTFSLTLGDY
                     ITPSLVGNVDFIGNVIYRNVLGLTNNLPFSAAYATVPLIVMAIYLALAKRVGAFEAL"
     gene            complement(21983..23032)
                     /locus_tag="VF225_02215"
     CDS             complement(21983..23032)
                     /locus_tag="VF225_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7144098.1"
                     /translation="MITKVGKLEGTLNLIAWEGYTYKQWVTPFVKKTGCKVHSKFGGS
                     SDEMVTLMRTGQYDAVSASGDASLRLILGKDVRPINPSLVPDYKNFIPALKSPSHNTV
                     KGVHYGISLQWGPNTLLYNTKKVKPAPNSWSVLYSSKYKGKVTVPDNAIQIADAALYL
                     SKSKPSLGITDPYELNDTQFKAAVALLKKQRPLVKKYWLTAGDEIDLFKNGGAVVGAS
                     WPYQTNTLQAAKAPVKDLIPREGATGWADTWMISSKAKHPNCAVSWIKWVSTPKVQAQ
                     QAIYFGETPANTKACKIMDQLSKGSCAQYHANASTAYFNSIKFWKTPVKDCGNGKSNC
                     MDYTKWQAAWTDIKS"
     gene            complement(23128..>23984)
                     /locus_tag="VF225_02220"
     CDS             complement(23128..>23984)
                     /locus_tag="VF225_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144099.1"
                     /translation="GFERPDEGSVELAGADVTNRAPYERDVNTVFQDYALFPHMTVAE
                     NVGYGLRVKRVPRAERKRRVEEILEVVHLPELGNRKPVQLSGGQRQRIALARSLVNTP
                     RVLLLDEPLGALDLKLRQEMQIELKRIQREVSNVGITFVYVTHDQEEALTMSDRLAVF
                     NEGRIEQIGAPAEVYERPATEFVAGFVGVSNVLERDGRKFTVRPEKIRMHFDEAGEGE
                     LGRIRDVVYVGMFTRYVVELDSGGELTVVKQNLETSSQEALESRGRRVRLEWRPEHTF
                     EIQRGGQL"
BASE COUNT         4049 a         7889 c         7937 g         4109 t
ORIGIN      
        1 gcgacgcggc gatatcgccg tgccctcgag acgacaggtg tggccgcggc cgccatcatg
       61 atcccctggg cggtgctcgg cttccgtgat ttcgccgaat acccgcgcgt gcttcgactc
      121 ctcgtgcgca atgaactgcc cacgagcttc tcgaccaaag ccttggcggg catgctgggg
      181 ttcggcccgg cagcgcttgc tttacagctc ggctccgtcg ttgctgtctt tgcactcgct
      241 cggcggcacg agggagatcg tcgcgcgttc tctgcagcga tcttcgctgc gctcgctctg
      301 tcaccgctcg tgtggatcca ctactacgtc ttgctgatcg tgccgatcgg ccttgccgcc
      361 cgccgcctga acgccgtctg gctgctaccc attctcgcgt tctgggttta caccgacaat
      421 ctgtcgtcgg tctggccggt gatgtgggtt tcgctcgtcg tgatgggagt agccgttctc
      481 acgctgcgcc cagctcgctc ggagcctcga ggttccctca gggtcgagga atacagacct
      541 gcgagcacaa gctcccgcag gatcggtcga aggtcggcgg agccgtcgtt gcgcgctacc
      601 acggccgcac gcccatgagg ccgcgaaccg cgccacccac ctgatccccg gcgcttcccg
      661 cccgcatttg aaaggctgtg ggagccgtcc atgcccaacc aactcgactt cgccctgatc
      721 aaacgactcc gcgaggtgct ggaccgccgg cccgcgacgg agaccgagtt gcgtacgctc
      781 aaggaacagg ccgaaggttg gcagcgtgcg gtcagcggac agctcgagtc gagcgagcgg
      841 cggctgctgc gactcaatgc gaaccccgcc agctccctgg ctcaaattgc ttccgaattg
      901 cgccgggtcg agaagctgcg gccgcagctc gacgaggtga ggggcttgct ggcggatctc
      961 gagaaccggg cccgtgagct gcggaccgaa tggttgctct cgcaggcgac gtcggcgaaa
     1021 gcttctagtc gacggccaga cggtcgccaa ccgtaagccc gcggcggtca gcctcgccgg
     1081 ccggaatctc cagcacggcc ttcgcgcccc ggcttcctgc gacacgccag ggccggagat
     1141 cggacgcgat cttgaccacg cgcaggtcgc ggtcgaggaa cacagcatcg atcggaaagc
     1201 gcatgaacgc catgtgcacc gacgatgccg gcttcaacag gattccctct ccgctcggga
     1261 gctcgcgacg tcccagcagg cccttcatgc gcgtgagcgc cgtgtccccc aggacacagc
     1321 gctcacacac gatgcttccg tcttcgcgac gaatcgcgac ttcggccact agaacccgat
     1381 atggatgtgg ttgtagtgat cggccatcgg gaacgacgct ccaccaagcc cgagcaatga
     1441 gatgacttgc cggggcatga cttccgtcgg gaggaacagg atgtcacgaa cggcgcgctc
     1501 ggtgatcccg ccgggctcct gatggccctg gatcggggtg tcgccgagcc ctgagacatc
     1561 aagggcgtgg ccggagatgt gtgccgagat cacgccgggc cgcgcataga ggcggtggcc
     1621 ggagaccagt gacgacacag tgacctgacc gaaggactcg cgcaggtacg cgatcacggc
     1681 caggacgcgg acgtcgacct tgttctgcac gatgtcgttc cgaccgccct cgtagatcga
     1741 gacgttcgga tccgacagga gccgcgtggc gatcgacttc ttcgccgcct cgaggccctt
     1801 gaccaggccc ttcaggccga ccgcgcgatc gtaccgggcc agtgcagcag ccctgtccgc
     1861 gaagctcgtg tcgcccgagt acgagacgat ccgtgcccag tcgcccttgg cgggcccgta
     1921 agacgagagc cgcccggcga gcttccggag cgtggggcgg ttggccggat tgtggccggt
     1981 tgcgccgcgc gcgcggagga tgcccagcat cgtcgcccag ttgaggcccg acgcctttgc
     2041 agtcgccttc aggttcgagg cgaacttcgg cgagagccga agagcaggcg gggtcggatc
     2101 ggggagctgc gagttgagcc agatcgtcgc tgcgctcggc ggtccctcga tctcgggagg
     2161 aggtgccggc ttcaacttcg gcaggaggat cgtgcgaagc ttcgtcgcct tccagccccg
     2221 ggcagccttc gcggtctgct tcgcggactg cttcgccgcg gtggcagtgg gtattgccgc
     2281 ggtgccggcc gtcgacgggc ccgtgaggct cgccgggggc gtggtgtcgt cgaccggtgt
     2341 cgcgtcggag gttgtgtcgg tcggggtcgc cgttgccgtt gcgtccgtcg tcgctgtggc
     2401 gtcggtgctc gcatcggccg ggccggcgcc ggcgtcgacg ggtgcagcta ccgccgctga
     2461 agtgtcatcg gtcgcgacgg gaggagtccc ggcatcggtc gtggtcgtat cgccggcgag
     2521 ctgctggttg tcttccccgg ggagggggcc gaactggttg ccagccacag cggcgagtga
     2581 cgcgcccgcg aagaagagcg tggtgaaaac gagcgttgtg gcgagccgac gcttcctcga
     2641 cttgggctcg ccggacgtgc cctctgcagg agtcggctgg ccgccgaact cccagttgtc
     2701 tctacggatg aaacgtcgtg gcattgccat tgctccttcg gtaaagcgcc ctggccgcca
     2761 cttgggcgcg gaatctgcgt tgttccgcag ccgcttacgg ttttttgact atcgatcccc
     2821 accgggccca gccaactccc tcacttaagg gaacgtcgcg gcggatcacc cgggtgaggg
     2881 atacgcgctt gccggtctcg aggcgatgaa tttgccgaga ttgaccggaa ggcgaagcgg
     2941 cgcgcctaga gagaaaggac gcaccagctt gtcacgcttt tttgaccgac ttcgagtcga
     3001 ctcaccatct accaccaatc tgctttgcat agcgcgccag cgcgcgcccg aaatggcgaa
     3061 ggggggtgaa tccatgtcgt tcaaggatct ggtccattac ctccgcgcac gcttcgccgt
     3121 cgaggagggt cagacgatgg ccgagtacgg cgtcgtcctg gcagtgatcg cggtcgcagt
     3181 tgtcgtggcc ctcggcttgc tctccacgtc catctccgcc gcattcgatg cagttcgcac
     3241 caagctgtag ttcgcagtcg taaccgagga caggagtttg accgtgaagc atcaccgcag
     3301 gcatcgaatc cgtgagttcc tcacgtttct ctcgcgtgag cacgctcagg cggagcctga
     3361 caagacggtc acgctcgcgg tggtcagcgg gagctcgttt ctcgcattca cccgcttctc
     3421 ggagaacggt gcgagtgcag tgaccagctt ggtgcggcta ttcacatagc cgcccaggca
     3481 tcagcggagc cctctcctgg cggcgagggc tccgcaaatc tttcgaagtg ggagccgaaa
     3541 atgcatctga aggacacaat caagaacgag cgagggcaga cgatgacgga gttcgccttc
     3601 gtcctgccga tcctgctcgt ggtgctcttc ggcatcatcc agttcggcat catcttcaac
     3661 aactacgtcg cgctcacgga tgcagcacgt gctgcatcgc gtcagggcgc ggtttcgcgt
     3721 aacgccgctg atcccgaggg cgcctgcgag gcgacgggat acagcgccgg agcgaatctc
     3781 gatcagccgc atgtgaagtt catcctcacg tgcgactcct catgggcggc ggggtcggac
     3841 gtcaccgtca ccgctaccta cccctacgac atcaagctgc tcgactgggt cgttgcgagc
     3901 gggactctca acacgactat gaaggagcga gtcgaatgag cgtccgcaag agcgaaagcg
     3961 gtcaggcctt cgtcctgatg gccctttccc tcgtcgtgat catggggatg gcggcgctcg
     4021 tcgtcgacgt tggcaactgg tatcacacga agcgccggct ccaggcaacg gccgacgccg
     4081 cggccctcgc gggcgcgcag ctgctcccga aggattctgc cggagcccag gcaatggcgt
     4141 tgagctacgc caacaagaac ggcggcgacg ttgctggggc ggacatcgcc gtgacctcga
     4201 cggccgtggc ggacgacacc atcggcgtcc gggccaggaa gacggacgcc ggcgtcttcg
     4261 gcagcgttct cggcattgcg agcgctgaca tctctgcgcg cgcgaaagcc cgtgtcgggc
     4321 ccccgctcaa ggcgaggtat gtcgctccga tggtggtctt ctgcgaccac cccttgatcc
     4381 acaactgcaa cggggacagc actccggact tcgggagcga cacgaccatg gactacgacc
     4441 cgatgggagc ccccggcgcc ttcggcatgc tgaatctcga cggcgggaac ggcacgcccg
     4501 gctcatcgga cgaggccgac tggatcctcc acggcttcaa caagtatctc gggctcgggg
     4561 actaccgctc ggatccgggc gcgaagttct cctcgagcaa cattcaggac gctctcgagg
     4621 ctcgtagggg cagcgtcctg ctcttccctg tctacaggca actcgacggt caaggtcaga
     4681 acgctgcgta cgagatcatc ggctggatcg ggtttcacct cacgagctac gaagtgcacg
     4741 gcaacaactc gactcttcac ggctacttca cggaattcat cgcccagggg atcctctcgg
     4801 aaggcggcgg cgacggggga ccctcaagtt ctttcggcgt gaagtcgatt caactcatcg
     4861 agtagcacca gtcctcaggc ccttctaagg aaaagagaaa ggactatgaa ttatcgcgcc
     4921 aagaacatcg ggatcgccgt cgcattggcc gctctcgccg ccattttgac gagcgtatac
     4981 gtcgtcaact acaagcgcca cgtgcagaac ggggaaggca aggtgacggt cctcgtcgcc
     5041 gcgcgcgaca tcccggcggg tacttcaggc gcggacgtag tcgaccagaa gatgctcaag
     5101 gagcagaccg tgccccgcaa ggcagtcgtc gcgggcgcga tctcgtctcc ggaccagctt
     5161 gcacagtacg tcgccaccca ggatgtcttc gagggtgagc aggtgagcac caggcgcttc
     5221 tcgcccccga cggagcaggg catccgcgca cagatcaagg gcactcagcg tgcctatcag
     5281 atcacgggcg atgcgcatca gctcctcgcg gggacgatcc aggccggcga tcacgttgac
     5341 gtcgtcggga cctggggcac aaattcgggg caaggggcga atgcttccac taacagcggc
     5401 ggccccgtgg ctcgcgtcgt cctgcgcaac ctcctcgtgc tgcgggcacc tggaggcgtg
     5461 ggcgcaaccg ccaagttggt ggaggggagc ggcgaatcgg tgcagctccg cgtgaccgat
     5521 tcccagtcgc agaagctcat ctggatcgaa aagaacggca aagagggggg ttggcacctc
     5581 gagttgcgcc cgccgtccaa ctcgctcgac agctcgaaca ccgtgcagga cagtagaacg
     5641 atgctcttcg acggacccgg aaggaggccg agatgagctc cgctaccgaa acaacccagc
     5701 gcgtgcgtgt ttacgccacc ggatcctgcg aagggttcga caagctccgc gagtcgctcg
     5761 caagccatcc cgagatcgag ctggtcgggg ccagcaccaa cgtcgccgac ggcgccgcgg
     5821 cgttggcggg cggccacctg gacgccgtcc tccatgcaac ccgggctgcg tcgctgcccg
     5881 cggacgagct tgcggcgatt cgggagcact ctcgtgcccc gatcctgatg ctcgcgtcgg
     5941 gcggctcttc ggggctgctc gagcaggcac tcgatgcaga cgtcgccgac gtcctgcttc
     6001 tgcctcagct cgtcgagaac atcgtcttcg caatccgcaa ggcgacgcac acgacccgcc
     6061 ggcatcacga caccggctcc ggccggcacg gcaagatcgt gacggtcttc tcgccgaagg
     6121 gcgggaccgg taagacggtg accgcaacca acctcgccgc tgcctgcgcg aagttcgagg
     6181 gccgcaagac gctactcctc gacctcgatc tgcagttcgg cgacgcggcg atcatgctcg
     6241 gcatcgagcc cgacaagacg atctacgacc tggtcgtcgc cccgggcgag ctcgacacgg
     6301 aaaagctggc cggttacacg accaagcacg cgtgcgggct cgaggtgctg ccggctccgc
     6361 ttcggccgga ggatgcagag ctcgtgacgg aggcaaagct cggtcgcctg ctcgaggtgg
     6421 cccgcgagtc gttcgacgtc atcgtggtcg acacgtcacc gttcttccac gggccgatgc
     6481 ttgcgacgct cgaccgtacg gacgagctct tgctgctctg ctcgctcgac gtgccgacgc
     6541 tgaagaacct acgcctggcg ctgcagacgc tcgacctgct gtcgttcccg aagcagcgtg
     6601 tccgcatcgt cctcaatcgc gccaactcca aggtcggcat gaagccgaac gaggtggaag
     6661 gtgcgctggg aatgaaggtg cgcttcgagt tgccgtccga ccgggcagtt ccgctttcgg
     6721 tcaaccgcgg caatcccgtc gtgctcgccg aagaaggcgc ggacgtgtcg aaggcgatca
     6781 aggcgatggc gaaggagctc tttccggcag tcaaggaaca cggcaagaag cgtcgtctcc
     6841 ttcccgcact cgcgaggtcg taactcatgg gcctccacga ccgcctcaaa acctccaacg
     6901 ggggctcctc cgcaaccgcg gagctgctca ccggcgggca cagccatgaa acctcgtccc
     6961 catctcctac acaggacccg tatgccgagc tgaagacccg catccaccat gcctgcatcg
     7021 ccacgcttgg tgcggagctg ttcaaacgcg aggcaaccgg cgatatgggt gaacgcgtca
     7081 cgcgtgctgt cacggagcag ctcgtgctgg accgaacgcc gctgacgcgc gaagagcgtc
     7141 gtcagatcat ccgggagatc accgacgaca tcctcggcta cgggccgctc gagacctttc
     7201 tccgagacga ctccgtcacc gaggtcatgg tcaacgccta cgatcagatc tacgtcgagc
     7261 ggctgggcaa gctcgagcgg gtgcaagcct cgttcgccga caactcccac ctgctgcgca
     7321 tcatcgacaa gatcgtctcg cagatcggca ggcgcgtaga cgaggcctcg ccgatggtcg
     7381 acgcacgtct tccggacggc tcgcgtgtga acgcgatcat cccgcctctt gccctcaagg
     7441 gcccgacgct cacgatccgg aagttcgcgc gcgatccgta cacgatgaac gacctgatct
     7501 cgttcggctc gatctcgccg aaggcggcgc agttcctcgc cgcctgcgtc aagggcaagc
     7561 tgaacgtgtt gatctccggt ggcaccggca cgggtaagac caccacgctg aacgcgatgt
     7621 ccgcgttcat tcccggtgac gagcgcatcg tcacgatcga ggatgcggcg gagctccagc
     7681 tccagcagga gcacgtgatc acgctcgaat cgcgtccttc gaacatcgaa ggccagggcg
     7741 aggtccgaat ccgcgagctc gtgcgcaacg cactgcgcat gcgccccgac cggatcatcg
     7801 tcggcgaggt ccgcggtccc gagacgctgg acatgctcca ggcgatgaac acgggccacg
     7861 aaggatcgct gacgacgatt cacgccaact ccccgcgcga cgcgctctcg cgtctcgaga
     7921 ccctcgtgct gacggcaggc gtcgagcttc ccctgcgcgc catccgcgag caggtttcga
     7981 gcgcattcga cctgctcgtc cagatcacgc ggcttgtcga cggctcgcgc cggatctcgc
     8041 acgtcaccga ggtcctccgc atggagtccg aggtgatcac cttgcaggac gtcttccacg
     8101 caaagccacc tgacgagcag acggccgcga gcggcgtcgg cctgaggctg ctctcccctc
     8161 tgagctgcac gggcctgaag ccgaacttcc tcgagaagat ggcggccaac ggcgtcgtcc
     8221 tgcagccgag cttcttcagc agggaggacg acacggacgc tccggcggtc ggccagggac
     8281 acgaggcatc gaagttcaag ggagctttcc aatgagccga ttccggctcg ccgtcgtcgg
     8341 gctcgcgttc atcctcctcg cagttcctgc cgccgcgggg gcggcggttc aactgcgggg
     8401 cgtcgacgcc acgtcgtacc cgactatccg cgcatccatc tacacgccta ccggcagcgg
     8461 caggcctgct ctctgggaga acgggacccc ggtcgccggc ttccaggcgc aaaacctcgg
     8521 cagcgtcaag gcgatcgcga ctctgatcga ccgctcgcag tcgatgcggg gcaagccgct
     8581 ggcagacgct gcggcaggtg cacgtgcatt cgtggcggcg aagcagcccg ccgacgagct
     8641 gtctgtgatc gccttcggct cgaaggtggc gacgctctcg ccgttctcga ccgtcaagct
     8701 cgacgcggat ctcccgctgg cgcgcatgca gacggatcgc gtcagaggga ccgcgctcta
     8761 cgacgccgtc attgcggcgg cgaaccagct caagggaaac cccctgccgg gtcgcgtgat
     8821 catcgtcctc agcgacggcg cggacaatgc gagcacgggc acgctcgcga ccgcgatcaa
     8881 gacggcggcc gaggcgaatg cttcggtcta cgcgatcgga atcgagggcg cgggattcac
     8941 gtccgacccg ctcgtgcagc tcgcaagcgc gacgggcggc cagtactacg gtgcgtcgtc
     9001 cacctccgag ctcgcacggg tctacgcctc catcgccgct gccctgaaga agacgtggcg
     9061 cgtccagtac gtcaccgccg cgcggccggg ggacagcgtc cggctgaccg cgtcgatcgt
     9121 cggtgcgggc tcgaccacac agctcgcgaa gattcccgcg ggcgatgcga tcgccgcgcc
     9181 ccaaccgacg aacctccttc cgaagaacgc ctatggcccc ggcggcccgc ttgcgatcgc
     9241 gatcgcggtc gcggcgctcg tgctcttcgg gtgcctcttc ttcctggccg gcatccgggg
     9301 ctcatgggtg cgcagccgaa tcgtcgcgca caccggcgag ggcaaggtga acgccaagaa
     9361 gaaccgcaag gagcggcgca acgaggcatt ctcgtccatc ttcgcggcca ctgagaaggc
     9421 gctgggcgac ctgaagcact ggcgagcgat cagccgcatg ctcgaacgcg ccgatgtgca
     9481 gctccggacg gtcgagttcg tctggatcac cgtcggggcg gccgtactga tggcgctgct
     9541 cgccctgctg ttcggggcct ctcccgttct tcttcttgcg ctgacggtga cggccggaat
     9601 gattccgttc agctgggttt ggctcaagat gcggaagcgg ctgaaggcct tcgaggacga
     9661 gctgcccgat cttctgatca cgatcgccgc ttcgctcaag gccggtcact ccttcaagca
     9721 ggggctccag gctgtcgtcg acgagggcca tcccccggcg aaccaggagt tcaagcgggt
     9781 actcaccgaa gcgtctctcg gccggcccat ggacgacgcg ctgctggaga tggccgagcg
     9841 catggcgtcg aagaacttcg agttcgccat cacggcggtc acgattcagc gacaggtcgg
     9901 cggctcgctc gcgaccctgt tcgacatggt cgccgacacc gtgcgccagc ggcagcagtt
     9961 cacacgcaag atccgcgcgc tcaccgcaat gggccgaatg tcggcctaca cgctgatcgg
    10021 catcccgttc ttcctggcgg gcgtgatcac gctcgtgaac aggaactaca tgcacccgct
    10081 ctacttctca cacacgggtc acatcgtcat gatcgtcggc ctcgtgatga tggggatcgg
    10141 ttcgctcatt ctgaaaaaaa tcgtctcttt ccgaggttga tgacatgtcg atgatcctga
    10201 cccttgcaat cgcagctatc ggtggggccg tcttcttctt tgcggagctc gccaccacgc
    10261 cgatgcgaaa ccggcgaaac ctcgtgcatc gtgcggccaa ctacggccgc ctccgtaccg
    10321 tcaccggaaa ggagatgccg cgcttccgcg agcgtgcgct cgacccgttc gtggcaaagg
    10381 cggcccggct cgtgctgcgg atcaacccgc gtatgtcggt ggaggccgtg tcggagaagt
    10441 tgatggcggc cggtatgcgg aggacgtccc cgacgacgat catcggtgcg aaaggcatcc
    10501 tcggcctcgg gggagtcatc ctcggccttg ccctcggaag cgcgagcgcg ccgaagtaca
    10561 tgttcctcat agcgttgttc ttcggcctca gcggagctct ggctcccggc ttctatctca
    10621 acagtcgtat gaagtcccgc cagctggcgg tgtcggccga gcttccggac gcgctcgacc
    10681 tgctctccgt gagtgtcgag gccgggctcg gcttcgacgg cgcggtccag aagctgaccg
    10741 agcacatgga cggcccgctg atcgaagagt tcgagctcgc actcggcggg atccgtatcg
    10801 gcgagggccg gagcgaagcg ctcaagaaga tgtccgagcg cagctcgtcg caggagatgg
    10861 ccagcttcgt gcgggcgatc atccaggcgg atcagctggg catctccctc ggccggatcc
    10921 tcaaggtcca ggcgggcgat acgcgactca agcggcagct catcgccgag gagaaggcaa
    10981 tgaaggcacc gatcaagatg ctcttcccaa ccgtcatctt catcttcccg gcgatgttcc
    11041 tcgtcgtgct cgggcctgcg ttcctcaacc tctcggcgct cttcaagttc tagaggagag
    11101 gtggcggcag tggccgaaca tggcaccgaa ggcggtttcg gcaccgggct gagggcgaag
    11161 ctccagtcgg gtgagacgcc gcccgagcct cggtcgccgg cggaagcgat cgccgcggca
    11221 tcgccccttg tcgaggagac cgaggccgaa gccctccgcg ccgagctgaa cgcgtcactc
    11281 gcgcgcgagc agcagctgcg ttcgtctttg cacgatcaag tggacaccgc cgcccgcgac
    11341 gagcagctcg agcaccagct cgcagagcag tcggccgggc tcgatcgccg ggcagcggcg
    11401 ttggcggaga cccaggccga gctcgacgag cgcgagcgcc gaatgaccga gcgtctggcg
    11461 gagctggacg gcttgctcga ggcgagggaa gagctcacga agctcgaggc gcggctcgca
    11521 gagcgcgagc agctcgtcga gctgaaggtg caggagctca aggtcggcga cggcgagcgt
    11581 gcggccactg ccgccgaggt ggaagagaag ctcgccgaga tcaccaagcg cgagcaggac
    11641 ctcgccaagg cagaagcgcg tgtgtcggcg gccggcgaag agaccgagac gcgccaggcg
    11701 aagctgatgc gcgcactcga ggagcgcgag gagaaggctc gggtaaagga cgagcaggcg
    11761 cgagccctcg agaagagggt cgcggagcgc gaagcacagc tggctgcgac cgaacgtgag
    11821 ttcactcagg caagggtgca actcgagacc cgtgagaagg tcatcggcaa gcgggaggca
    11881 gcgatctccg ccgagagtgg gcgagacgtc gagcagctgt ccgctcgagc acgcgagctc
    11941 gacgagcgcg agctcgagct ctcgactcgc gcaaacgacc tcactgctcg catggaagac
    12001 ctcgagcgcc aggccgccga acgcgatgcg gtcgcaaagg acgcgctcgc caagctcgac
    12061 agcatcgaac aacgcgagac cgagctgatc gcgcgcgagg ccgagcttca tcgactggaa
    12121 gccaccgccc ggcgagcggc cgaggaggcg cgcaagcccg aggtggacga gctcgccgag
    12181 cggcgcctga agcagcgtga cgacgaactc tccgcccgcg agtctgccct caacaaggct
    12241 gagcaggcca tcctggcgtc gcgcagccgc tacgagggca aagagaaggg gctcgacgag
    12301 cgcgagaagg tgctcgccgg ccgtgcgagg gagctcgagg agcgcgacgc cgcgctcaac
    12361 ctgcgcgagg cccagatgga agccgagctc gaggtccggg agcagcggct cgagcagcgc
    12421 gagaaggcgg gcgccgagct ggaggccgct ctgtcgacgc gcgagagcga cctctccatg
    12481 tacgtctctc agctgcagga caacttcgac cgccgcgaat ccgaatggtg gtcgaaacag
    12541 ctcggcaagg acgccgggca gcaacccgcc gcgtgacggc gtagccaccc gtcagaatcc
    12601 gcggcatggc gcagacgctg agtgacagtg agctcgtcgc ccgttgccgg tccggtgacc
    12661 agcaagcgtg ggccgagctc gtcgaccgct tctcacgcta cgtgtacgcc atctctgtcc
    12721 aggcgttccg gttgtccgat gccgacgcgg aggacgtgtt tcaggaagtg ttcgcgcgtg
    12781 cgtacgaaca tctcgacggg ctgcgggacg acgccgccgt ccggccctgg ctcgcgcagc
    12841 tcaccaggcg gctgtgcatc gaccggctcc gcgccacctc acgcgagcgt ccggccacgg
    12901 acgaagatct cgagctcgcc gggtctgagg aaaccctgac gatgctcgag gacgcgctca
    12961 ccgttcacga ggcccttgct gccacccccg agcactgccg ggagatcctc gatcgcttct
    13021 tcgcccgcga cgagagctac cgcaccatcg gcgaggcgct cgatctcccg gccggaacga
    13081 tcgcgagccg catctccagg tgcctggggc ggctacgtga gttcctcgag ggaagaactc
    13141 agcccgatcc cccgtctagt tcccgatgac cgcttttgac gaggagcgcc ttgcgcgcct
    13201 gattcgagct ctgcccccgg ccccggaggc ttgggtacgt gccgcgcagg agttgccgct
    13261 ggccaaagcc gggctcgacg agatcgtcgc ccgcgcggag gcggacgctc acttcaaggc
    13321 ccggctcgtg gccgatctcg agtctgccct cgaggccgaa ggctatgagc gcgatcccgc
    13381 ggtcatcaaa gcgctgaagg ttcgcttcaa gtccaagtag cgcttctagg acaatcccca
    13441 tatggagctg ctcgacctgc gcctgcggga attcctcgac gagatcgcag gcgaggggag
    13501 aactcccggc ggagggtcgg ttgccgcgct cgtcaccgcg atggctgccg ggctcctcgc
    13561 caaggtggcc cgccggtccg tggacacctg gccggacgct gcgggaatcg cggcgcaggc
    13621 cgagagcctt caggcccggg ccgctccgct tgcacaggcg gacgcggagc agtacgaagc
    13681 cgcgctcctg gcccgcgggc agagcgggga ggggcagggc gcgcggcagg acttcgccct
    13741 cggccaggcg tatgcaaagg cggccgagcc tccgctacag atcgcccggg cggcaggcga
    13801 cgtcgcgcag ctcgcactga ccgtggcaca aagcggcgac gcggccctgt gggccgatgc
    13861 agtgacagcg gccctgctcg cagcagcagc ggcgaaggcc gcggcggagc tggtggcggt
    13921 gaatctgacc gcatcggcag gcgatcgacg cgttctcgaa gcggcgaaag tggcggagga
    13981 ggccgcccgc gccgccgaag cggcgcgggc ggtgcgggag tagatcaggc ggtacgaacc
    14041 gccggccgcc gccgcacgat gcccctcagg gcggcggtcg atcggagagc ccacgtcctc
    14101 gcagtcgccg cgcgatccgg atccccctca cggatgctcg cggcaagccg ctcctgagtc
    14161 gcctcgcgta gcaggtccgc gtgacggtct cgagcgatct cttgatgcga gaacgaagac
    14221 actccggcca ctagacggcc ccggccccgc ttgtcttccc gcaggcggga ctcgtacgct
    14281 gcagggatgt ccgaggaact tccagcctgg agcctctccg cggaggccgt cgaatcgatc
    14341 gccctccccg aggaatggcc tgggcaggtc acgcgcgagt gggcctgggg cggagcgact
    14401 ggagccggtg cccgggtgtg cgtcctcgac agcggcatcg agaccgacca tccgctcgtg
    14461 ggggacgtcc agaagtcggt cgcgatctcg atcgagggtg acgaggtgat catcgaggac
    14521 aacgacaccg cgggggacct ctgcgggcac ggcactgcct gcgccggggt gatccgctcg
    14581 ctggcacccg actgcgagct cgtgagcgtt cgggtgctcg gggccggcta taccggcagc
    14641 ggcgccgtcc tgctggaggg cttgcgctgg gcgatcgcgc agggctacga cgtgatcaac
    14701 atgagcctct cgacgaccaa gcagaagttc gcgtcgctct tgcacgagct cgcggacacc
    14761 gcatacttcc gtcgcacgat gctcgtcgcc tccgctcaca acatgcccgt cgaaagctat
    14821 ccatggcgct tctcgagcgt catctcggtc gggagccacg agcaggaaga cccttaccag
    14881 tactactaca accctgctcc gccggtcgaa ttcttcgccc ggggcgtcga cgtcgaggtc
    14941 gcgtggaacg gcggcggccg tctcgtctgc accggcaaca gcttcgccac cccgcacatc
    15001 gccgggatcg cggcgctcgt cctctcgaag catcccgagc tgacgccgtt ccaactgaag
    15061 agcgtcctct acctcactgc gacgaacgtg ggaggcgaga agtgagcgac gagcttcggg
    15121 cggcggtcgc cgcgggcgtc ttgggctcgg aggaccaata tcgcgccctg ctccagtcga
    15181 tcgtggacgt cgcccgcgcg atcttcaggg cgaaggcctc ctcgatcctg ctcctcgacg
    15241 aggaagcgga cgagctcgtc ttcgaggctg ctgcggacga ggcgtcggaa gcactcatcg
    15301 gaaagcggtt cccctcgagc accggaatcg caggcttcgt gctcgtcagc cggcagccgc
    15361 tcgtgatcga ggacgtgctc accgatccgc gcttctcgcg cgagacggcg gagagcaccg
    15421 gtttcgtccc gaagggcttg atggcggttc cgctgctcca cgaggaacgg gtgctgggag
    15481 tcctcgaggt gctcgatcgc cctcaagaca cgcggttcac cctcgccgag atggagctcc
    15541 tcgggctgtt cgccagccag gcggcgatcg cgctcgacct tctgcaacgg gctcgtcggg
    15601 cgcaggaggc gctggccggc gacggagagc tcagcgtcat ctcacggctg gccgagaagc
    15661 tcgaggctcg tcgggaggag ggctccgaga ccgccctgcg cctcctggcc gagctggaac
    15721 acctgctcta gaacgacaaa agcccgggga tcccgggctt ttgtcactcg atcgagatac
    15781 tcgttactta aagcccttgt gccgatgggc ctcgacgtcg tctgaatcgt cggagccggg
    15841 ctcttcgttg gccttgaagc ccttgtgccg atgggcctcg acgtcgtctg actcgggctc
    15901 tccggtggcc agaagctctt ggttggccag aagctcttcg ttggccttaa agcccttgtg
    15961 cttatgggcc tcgacgtctt cgtccttcgc ctcttcctgc tcgaagcgat cgtcggacat
    16021 gaactcctcc ttctagaagg tcttcggcat gatcgccgtt tcaggtacta gacgggctgc
    16081 tcacccggaa tcttccctgc tcctcttgaa atacacaagc ctgtgcgatt ctccgccgga
    16141 tgcggcagat tctaggccac ctggctgagt cgagccgggc atttcgggac gtcttccgaa
    16201 acccgggatt gcgccgaatc gagcttgcct gggcggcttc gatcctcggc acgtgggcgt
    16261 acgggatcgc agtcgtcgtc tacgcgtacg agcagggcgg tgccacggct gtgggcgtgg
    16321 tcgggctggc ccgctgggtc gccgctgcga ttgcgtcccc gtttgcggcg atccttggcg
    16381 atcgctacga ccgtcgctgg gtgatggccg gctcggacat cgcacgggcg gtcctgatcg
    16441 cgggtgctgc cctggccgtg ttcgccggtg ccccgccgat cgtgatctac gtcatcgcgg
    16501 gcggcgtcag cgtcgtggcc accgcgtttc gcccggccga ggccgccttg atcccatccc
    16561 tggcccggac gcccgaagag ctcacggccg cgaacgtcgc agcgagcacg atcgagagcg
    16621 tcggaatctt cggcggcccg gcgatcggtg ggctgttgtt ggccgcggcc ggaacgggag
    16681 tcgtctttct cgttacgggt ggcgcgatgc tctggtcggc attcctcatc gcccggatcc
    16741 gccctggtat cgaggtggag gactcaggcg agcgggagcc ggtctcggtc ctggacgagt
    16801 tgctggcggg tttccgggcc attgcgcacg agcggaggac gcgtttcctg gtcgggctct
    16861 tttcggccca gacgtttgtg gacggaatgc tcagcgtgct catcgtggtc atcgcgctca
    16921 aactgctcga caccggccag gcaggcgtcg gttttctcaa ctcggcagtg ggtgtcggcg
    16981 gcctgctcgg ggcgcttgcg gcggcggcgc tcgtcggccg caggcggcag gccgcggatt
    17041 tcgggctggg gatcttcatc tggggcgttc ccatcgccct ggtggcgatc tggcccaatc
    17101 aggccttcgt ccttgtctta cttgcggtcg tcggaattgg gaacacgatc gtcgacgtct
    17161 cggggatgac gctgctgcag cgctcggcgc ccgatgacgt gctggctcga gtcttcggcg
    17221 tgctggaaag cgtcctgctt ctcgccggtg ccctcggcgc tgttgctgcg ccgctgctgc
    17281 tcaactggct cggtacgcgc ggcgcgctga tcgtcgccgg ctctctcttg ccactggtcg
    17341 tgattcctgc gtggccgcgg ctgaacgcga tcgaccgggc tgcggaggtt cccgtcgagc
    17401 ggctcgagtt cctccgcgcg aatccgatct tcgcgccgtt gccggcatcg accttggaac
    17461 agctcgccca tgccctcgaa gaggtgcctg cctcggtcgg cgaggagatc gtgcgacaag
    17521 gggagccggg tgaccgtttc tatctgatca agcacggtgg cttcgacgtg tacatcgatg
    17581 gagagccggt gcagtcgctc gataccggcg attcattcgg agagattgct ctgctgcgta
    17641 acgtgccgcg gaccgcgacc gtccgcgcgc ggaccgaggc cgtgctctat gcgctcgacc
    17701 ggaggcactt cctggcggcg gtcacggggt ttgggccgag cctctcggct gcggaaggga
    17761 ttatcgggat gcggcaggga acgggtcgcg ccgggatcgt gcgggcctag tctcgagcgc
    17821 gggctcggcg aatgtcagcc tgcgcgcatc cgcgtcgacc gtcgcggtca caccgagcgg
    17881 gatcgtgcag agatggtcac cgtgaccgag cgggaggtta tggaggatcg gaacgccgag
    17941 cggctcgagg tgatgctcga acacgtcttc catcgagcgg ctccgcagcc acggttccgg
    18001 ttgcaggtgc tcctcgcact tgtacatctc accgatcacg acgcctgcga tccggtcgag
    18061 tttgcccgca ttccggagct gcgtcagcat tccgtcgacg tgccagggcg ggcagtgcac
    18121 gtcctcgaag aagaggatcg catcgtcgaa gtccacctcc cacggggttg cgagcgtctc
    18181 gcgcaggagc cagaggcacc cgccgacgag cggcgctgtc gccttgccgg tgccgatcga
    18241 gccgatgtac ggatcgtccg gccggggtgg gagttcgccg agcggctccg aatccgtgag
    18301 tgcccgcagg aaccgctcct tcgaccagtc gccacgcttc gggctgccca tgcccgtgaa
    18361 gccagggccg tagaaggtga cgaggccggt gcgctggcgg atcgcgacgt ggagcgcggt
    18421 gacgtccgag tagccgacga gcggcttcgg gttctcggcg atcaggtcga agtcgaggtg
    18481 tgggacgacc tgcgaggcgc cgtagccgcc gcgcaggagc tggacgatgt cgacctcggg
    18541 gtcggcgaac agccgattga cgtcgcgtgc tcttgcctcc gcatcgcccg cgacgaaatc
    18601 gtcgcgggcg tagacgtgct cggcgagctt gacgcggtaa ccgcgttcct cgtaccagcg
    18661 aacgccgcga tcgacctcgg accgctcttc gaacggactg gacgcagccg cgacgccgat
    18721 cgtccctccg ggaaccagcg gcggcggcag caccagtcca gtcacttcag ccatcgtccc
    18781 cagaggctgg ggtcagaccc ttgcagttcg aggcgcggct ccaatgctct agtcgagttt
    18841 cgccatcacg tgcttgatct gcgtgtagtc ctcgagcgag tacgccgaca ggtccttgcc
    18901 gtagccggac tgcttgaagc cgccgtgcgg catctcgctc accagcggga tgtggtcgtt
    18961 gatccacacc gtgccgaatt gcagcttgcg tgaggcattc agcgcgcggc cgatgtcgcg
    19021 ggtccagacc gacgccgcca ggccgtagtt gacgtcgttc gcccaggcaa tcgcctggtc
    19081 gtcgtccgcg aaccgctgca cggtcacgac cggcccgaac acctcccgct ggatgatctc
    19141 gtcctcctgg ccgacgtcca cgacgaccgt cggcttgata aagaagcccc ggtcgccgtt
    19201 cgtgcctccg ccggtcaaaa ccgttgcacc cttcgcacgg tcgaggaagc cgagcacgcg
    19261 ttcctgctgg tccttcgaga tcaccggacc catctcgatc tcgtccccct cggcggggtc
    19321 gccgacatgc agcgactcga cggccggcac gagctcttcg aggagcttgt cgtagatctt
    19381 cggccccgcg acgactcgcg acgcggcggt gcagtcctga ccggagttcc agtacccccc
    19441 aatcttgatt ccctcggcca ctgcggccgg atccgcgtcg tcgaagacga ttaccggtgc
    19501 tttgccgccg agctcgagat ggacgcgctt cagattgtcc gccgcggtcc gcgcgatgat
    19561 cttcccggtt tcgacgtcgc ccgtgagtga cacgagacgt acgtcggggt gcttgacgat
    19621 tgcctcgccc gccggcacgc cgtctcccgt gatcacgttc aagacgcctg cgggcaggat
    19681 gtcagccgcg agttgcgcaa accgcagcgt cgtcagcggc gtctgctccg atggtttcag
    19741 cacctgcgta tttcctgcag cgagcgcggg cgcgaccttc cagacggcca tcatcagcgg
    19801 gtagttccac ggcgcgatgc cgcccacgac gccgatcggc tcacggcgga gccacgacgt
    19861 gtagccgcgc atgtactcgc cggcggactt gccttcgagc acgcgtgctg ccccggcaaa
    19921 gaagcgaatg ttgtccgagc agaccggcag ctcgtccctc gcagcggcga ggggcttgcc
    19981 gacgttctgc gactcgagtt cggccagctc ctccgtgtgc tcgtcgatcg cgtcggcgag
    20041 cttcagcagc atctctgcgc gctcgcccgg cgtcgtctcc agccactccg gcaacgcctt
    20101 cttcgccgcc tggacggcgc gatcgacgtc ctcggccgag gcgcgtggga cctcggcgat
    20161 ggtctcgccg gtggaggggt tcagaacctc catcgtctcg ccgccggccg cgtcgaccca
    20221 ctcgccgccg acgaagttct ggtgtttcga aacggtcgtg ctcacggtct acctccttcg
    20281 caggatcccc gtgtcgcgcg tcaggcgctg cgcgatcacc acgggaacca cggtcatcac
    20341 tatgacgaaa aagacgacga cgttcacctg cggtagctgc tggccgagcc ggagatttcc
    20401 gaggatccag atcggcagcg tgttctgcgc cccggcggtg aaggtcgtca cgatcacctc
    20461 gtcgaacgac agcgcgaagg ccagcaacgc tccggcgacc aacgcggtcg agatgacggg
    20521 aaacgtgacg aagcggaacg tctgccaacc gtcggccccc aggtccatcg aggcttcggt
    20581 caaggacgtc tgcgtccgcc ggagccgggc gaccacgttg ttgtagacga cgacgatgca
    20641 gaaggtcgaa tggccgatca cgatcgtcca cagcgaaggc gtcaggccgt ggatcgggtt
    20701 gttgttgaag aacgagttca gcgcgatccc ggtgacgacg cccggtagcg cgatcggcag
    20761 aacgagcaga aacgtgatcg catcccggcc gaagaagcga aagcggctca ccacggtcgc
    20821 cgcggcggtt ccgagcacga gcgcaatcga agtcgcgatg ccggcggcct tcaacgaaag
    20881 gacgagagcg ctacgggcct cttcgttgtg ccacgccagg tggaaccact tggtgctgaa
    20941 cccggcgatg ggccagctct ggatgttgga ggagttgaac gcgtagacgc agatgagaac
    21001 gagggggatc cagagaaaga ggacgatgag aactgtccag acgctgagcg acagccgtgt
    21061 tgcgcgggtc tccactacaa agcctcgaac gcgccgaccc gcttggccag cgcgagatag
    21121 atggccatga cgatcagcgg cacggtcgca tacgccgccg agaaggggag gttgttcgtc
    21181 aagccgagga cgttgcgata gatcacgttg ccgatgaagt cgacgtttcc gacgagtgaa
    21241 ggcgtgatgt agtcaccgag cgtcagcgag aatgtgaaga tcgagcccgc tgcgatcccc
    21301 gggagcgcaa ggggcacaat cacgcgccgg aaggtcgtcc agttccgtgc gccgagatcc
    21361 cgtgaagcct cgaggtacga atccggtatc cgttccagcg ccgcgtagat cgggaggatc
    21421 atgaatggaa gccagatgta cgagaacacg atccacatcg cggtactcgt gaatgcccag
    21481 tcttgctggg gtagcccgat cttcgcgagc gaccagttga gcgcgccgtc ctggttcagg
    21541 atcaaccgcc acgcgtagat ccgggcgagg tagctcgaaa acagcggcag cagcacgagg
    21601 acgaagagga gggttcgcag tcgcggcgtt gcaacccgcg ccatgtagta cgcgaacgga
    21661 aacgcgatca gcgcatccgt caaggtgacg agggccgcca tccagatcgt gcgccacgcg
    21721 atctggcggt aggtcgggtc gtggatgatc gtgtcgaagt tcgtccacgt ccagacgtgc
    21781 gtgagcttgc cggtgaagga gtcgacgctc cagaacgccg acacgaacag gacggccagc
    21841 gccgccaggt agaagaccag catccagccg agtggcggca gcagcaacgc gaccgcgcgc
    21901 aaccacggcc gccgccacag tgctgcggaa aggcgacggc gggccgaccg agaccgaccc
    21961 gccgcgtcat acgcctgaga cgtcaactct tgatgtcagt ccaggctgcc tgccacttcg
    22021 tgtagtccat gcagttgctc ttgccgttgc cgcagtcctt caccggcgtc ttccagaact
    22081 tgatcgagtt gaagtaggcc gtcgaggcgt tggcgtggta ctgcgcgcaa gagcccttgg
    22141 acagctggtc catgatcttg cacgccttcg tgttcgccgg cgtctcgccg aagtagatcg
    22201 cctgctgagc ctgcaccttc ggcgtcgaaa cccacttgat ccacgacacc gcgcagttcg
    22261 ggtgcttcgc cttcgaggag atcatccagg tgtcggccca gcccgtcgcg ccttccctcg
    22321 ggatcaggtc cttcacgggc gctttcgcgg cctggagcgt gttggtctgg tacggccacg
    22381 acgctccgac gacggctccg ccgttcttga acagatcgat ctcgtcaccg gcagtcagcc
    22441 agtacttctt gacgagtggc cgctgcttct tcagcagggc cacggccgcc ttaaactgag
    22501 tgtcgtttag ctcgtacggg tccgtgatgc cgaggctcgg cttcgacttc gacaggtaca
    22561 gcgctgcgtc ggctatctgg atcgcattgt ccggcactgt gactttgccc ttgtacttcg
    22621 agctgtagag aacactccag ctgttcggcg cgggcttgac cttcttcgtg ttgtagagga
    22681 gcgtgttcgg tccccactgc agcgaaatgc cgtaatgcac gcccttgacc gtgttgtgcg
    22741 agggcgactt cagagccgga atgaagttct tgtagtccgg cacgaggctc gggttgatcg
    22801 gtcggacgtc cttgccgagg atcagacgca gactggcgtc tccagaggcc gagaccgcgt
    22861 cgtactggcc ggtccgcatg agcgtgacca tctcgtccga cgagccgccg aacttcgagt
    22921 gcaccttgca ccccgtcttc ttcacaaacg gggtcaccca ctgcttgtag gtgtaaccct
    22981 cccacgcaat caggttcagc gttccctcga gctttccgac tttggtgatc atcgacgggc
    23041 tgctcgagtc cgaactgact cccgacgcgg gcacgcacag tgccgcggcc agcgccgcaa
    23101 gcagtacgtg agccctgcga atcctcctca tagttgtcct cctcgttgga tctcaaaggt
    23161 gtgctcggga cgccactcca ggcgcacccg cctacccctg gactcgagcg cctcctgcga
    23221 ggacgtctcg aggttctgtt tgacgacggt gagctcgccc ccggagtcga gctccacgac
    23281 gtacctcgtg aacatgccga cgtagacgac gtcgcggatg cgacccagct cgccctcgcc
    23341 tgcctcgtcg aaatgcatcc ggatcttctc cggtcgtacg gtgaattttc tgccatcccg
    23401 ctccaacacg ttcgacacgc cgacaaagcc ggcgacgaac tcggtggcgg gacgttcgta
    23461 gacttcggcc ggcgcgccga tctgctcgat ccggccctcg ttgaagaccg cgaggcggtc
    23521 gctcatcgtg agcgcctcct cctggtcgtg cgtgacgtag acgaaagtga tgccgacgtt
    23581 gctgacctcg cgttggatgc gcttcagctc gatctgcatc tcctgccgga gcttcaggtc
    23641 gagcgcgccc agcggctcgt cgagcagcag aaccctcggc gtgttcacga gcgagcgtgc
    23701 gagcgcgatg cgctgccgct gcccgccgga gagctgaacg ggcttgcggt tcccgagctc
    23761 tggaaggtgc acgacttcca ggatctcctc gacgcggcgt ttgcgctccg cccgcggaac
    23821 gcgcttcacg cggaggccat agccgacgtt ctcggcgacg gtcatgtgcg ggaagagcgc
    23881 atagtcctgg aagaccgtgt tcacgtcccg ctcgtagggc gcgcggttcg tgacgtctgc
    23941 gccggcgagc tcgacgctgc cttcgtcggg ccgctcgaag ccgg
//
LOCUS       DASVEU010000055         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_233015,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000055 DASVEU010000000
VERSION     DASVEU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233015"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF225_02225"
     CDS             complement(<1..373)
                     /locus_tag="VF225_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144100.1"
                     /translation="MSTAETLALGAIAGFTIFLGLPIGRMQNVTTGTKAFLAATATGV
                     LIFLFWDVMSGAVGPVETALEEGRGGRFAWLAFLLTAGFFTGLMSLVYYDTWMKRRRR
                     RAFLGPGAASTAEFEHDHHFTG"
     gene            407..1090
                     /locus_tag="VF225_02230"
     CDS             407..1090
                     /locus_tag="VF225_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7144101.1"
                     /translation="MAQGHSVDEYLETIYFLAFPIGEYRPLGSGSPTLASRVAEMLGV
                     SRASAGEMLKRLEGEGLIERGEHKEALLTAKGRKRAERVVRKHRIIERLLTDFMGYTA
                     AEAHVQADLLGDTFTEDMVQRIEERLGHPDRCPHGWPVDPDVEQAENRDLAPLSDLKA
                     GARATIVRLAEHDGDLLHWFYKEGLVPGKEIEVRDAQPAAGQVTVRLNGQERAIGEKA
                     AAGLFVRPG"
     gene            1368..>2685
                     /locus_tag="VF225_02235"
     CDS             1368..>2685
                     /locus_tag="VF225_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144102.1"
                     /translation="MSAVPTTPAQTAAEAVAFPRVLARSLALPLIFVAATAYHFLQSR
                     GHATTTVFNDELLYAKLSQSIAAGHGLSIRGEHFFFPAPLASFVQAPAWLISSLPDAY
                     AVAKFLNAAVMSAAVFPAYWLARQVVRPSFALLTAAAAVATPAMFYHGYLMSEALAYP
                     IFLVTVAVLARALAAQSKRLVLEVPAVCVLAIATRVQFLVLPLAYLVGVAVCGRGAYR
                     RHLVPAGLTALLVTALVGLPGVLGQYGQAGTQVGHAPGAIAHWAGTNGILLVYGLGLA
                     VVPGAIFGLGLMLGRPRTPIERAVAVLTVVCTIFFVGQSSLIGAGEAQRPLERYLFYV
                     TPLFFLAFFAYAERGAPRRFLYGGLGCIGAVLLSLVSLPGMTGTAAFFFDAPTLTGFA
                     RAAYQFGFPNAALLYGALPLALALLASVVPLTRRGAPHFFALVAIGL"
BASE COUNT          388 a          937 c          882 g          478 t
ORIGIN      
        1 tccccgtgaa gtgatggtcg tgctcgaact ctgcagtgga cgcagcgccc ggcccgagga
       61 acgcgcggcg gcgtcggcgc ttcatccagg tgtcgtagta cacgaggctc atcaggccgg
      121 tgaaaaagcc cgcggtgagc aggaacgcga gccacgcgaa ccgtccgccg cgcccttcct
      181 cgagggcagt ctcgacgggc ccgacggcgc cgctcatcac gtcccagaac aggaagatca
      241 ggacgccggt cgcggtcgcg gcgaggaacg ccttcgtccc ggtggtgacg ttctgcatgc
      301 ggccgatcgg cagcccgagg aaaatcgtga agccggcgat cgcgccgagc gcgagagtct
      361 ctgcggtgct catgtaaggg tcaccttaca ggttacgatc agcggaatgg cccagggaca
      421 ttcggtcgac gagtacctcg agacgatcta cttcctcgcc tttccgatcg gcgagtacag
      481 gccgctcggc agtggttctc ccacgctcgc atcgagagtc gcggagatgc tcggcgtgtc
      541 gcgtgcctcg gccggcgaga tgctgaagcg gctcgagggc gaagggctga tcgaacgcgg
      601 cgagcacaaa gaagcgttgc tgacagcgaa gggtcgcaag cgcgcggagc gcgtcgtccg
      661 caagcaccgg atcatcgagc ggctgctcac cgacttcatg ggctacacgg ccgccgaggc
      721 gcacgtgcag gccgacctgc tcggtgacac gttcaccgag gacatggtgc agaggatcga
      781 ggagcgactc gggcatccgg accggtgccc acacggctgg cccgtcgatc ccgacgtcga
      841 gcaagccgag aaccgcgacc tcgcgccgct gtcggatctg aaagccggcg cgcgcgcgac
      901 gatcgtgcgc ctcgcggagc atgacggcga ccttctgcac tggttctaca aggaagggct
      961 cgtcccgggc aaagagatcg aggtccgtga cgcgcaaccg gccgccggcc aggtcacggt
     1021 gagactgaac ggccaggagc gggcgatcgg cgaaaaagcc gccgccggcc tcttcgtgcg
     1081 acccggctag ttccgacgcg gctactcggg acccggtggg gactgtccca accatggcca
     1141 gtcgcgccat ggctcgagct ggtcgaacga gacctgtact tcgtgccgga agtgtgactt
     1201 tttctcccca ggccccccgg aaccggctca accacgccat cctgaggggc gggggccgcg
     1261 ctcttcgtcg cgggacatgt ccgcggcggg cggcaacgtc cttcgcttct gtgcgccctg
     1321 acagcccctg tctaacggca accctccccg gagccgatac gaccctcgtg agcgccgttc
     1381 cgacgacgcc ggctcagact gcggccgagg ctgttgcgtt cccgcgcgtc cttgcgcggt
     1441 cgctcgcgtt accgctgatc ttcgtcgcgg ccaccgccta ccacttcctg cagtcgcgtg
     1501 gacacgcgac gacgaccgtg ttcaacgacg agctgctcta cgcgaagctg tcacagtcga
     1561 tcgccgccgg acacggactg tcgattcgcg gggagcactt cttctttccg gcgccccttg
     1621 cctcgttcgt gcaggctccc gcctggctga tctcctcgct gccggacgcc tacgccgtcg
     1681 ccaagttcct gaacgcggca gtcatgtcgg cggcggtctt cccggcgtac tggctggccc
     1741 gacaagtggt tcgcccgtcg ttcgcgctgc tcaccgcggc tgctgctgtg gcgacgcccg
     1801 cgatgttcta ccacggctat ctcatgtcag aggcgctcgc ctacccgatc tttctcgtca
     1861 ccgtcgcagt tctcgcacgc gcgctcgccg cgcaatccaa acggttggtg ctcgaggtcc
     1921 cggccgtgtg cgtgctcgcc atcgcaacgc gggtccagtt cctcgtccta ccgctcgcat
     1981 acctcgtcgg cgtcgccgtc tgcggtcgag gcgcctatcg acggcacctc gtccctgcgg
     2041 gattgacggc gctgctcgtc acggcgctcg tcgggctccc cggcgtgctg ggtcaatacg
     2101 gccaggcagg cacgcaggtc ggtcatgcgc ccggcgcgat cgcacactgg gcgggcacga
     2161 acggaattct tctggtctac ggcctcgggc tcgcggtcgt gccgggcgcg atcttcggtc
     2221 tcggcttgat gctcggacgc ccgcgcacgc cgatcgaaag agccgtggca gtgctgaccg
     2281 tcgtctgcac catcttcttc gtcggccagt cgtcgctgat cggcgccggg gaggcgcagc
     2341 ggccgctgga gcgctacctc ttctacgtca cgccgctctt cttcctcgcg ttcttcgcgt
     2401 acgcggagcg aggcgcaccg cgtcgatttc tgtacggcgg tctcggttgc atcggggcag
     2461 tcctactttc gctcgtgtcg ctgccgggga tgaccggaac ggccgcgttc ttcttcgacg
     2521 cgcccaccct gacgggcttc gcccgcgcgg cctaccagtt cgggttcccg aacgccgctc
     2581 tgctctacgg cgcactcccg ctggcgcttg cgttgctcgc ctcggtggtg ccgctgacgc
     2641 gccgtggtgc gcctcacttc tttgcgctgg tggcaatcgg cctca
//
LOCUS       DASVEU010000056        12068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1593223,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000056 DASVEU010000000
VERSION     DASVEU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593223"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF225_02240"
     CDS             complement(<1..584)
                     /locus_tag="VF225_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144103.1"
                     /translation="MTAVWTIAGYGLREALRRKVFVVVCFLTIAFLALYWLGARYAFH
                     HVANVAPPEGVDARTFAGAFLFGMAMFGTLFLGVVLAIFLTLGTVRGDAERALLQPLV
                     VRPIGRSTLLVARFLGAGAVCVPYVLILYFTAMVLTGLEGSWWPDRIVTPGLELAAGV
                     LIVAALSLLGSVFLSSIANGIAIFMLFGAGLVAGL"
     gene            complement(581..1423)
                     /locus_tag="VF225_02245"
     CDS             complement(581..1423)
                     /locus_tag="VF225_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144104.1"
                     /translation="MSAALAVSGLVKRYGAVEALGGVDLEVGDSELVGLLGPNGAGKS
                     TLVKIACGLVRPTAGRVEVCGARAGSREARAALGYLAELFRFPGWMTADELLELHQRL
                     AGSRGGERERVELLELVSLHDARRRPVEAMSKGMQQRLGIAQALVGSPRLLVLDEPTS
                     ALDPVGRRTVRTLLEELQRRGVSVLLNSHLLSEIELVCDRVVILLGGRVVTAGTPAEL
                     ARPRGVEIETDAGKRTYPEAGRDDMPRLVAELVRDGKSVYGASLLTSTLEDVYVEAVG
                     GEGS"
     gene            1448..1699
                     /locus_tag="VF225_02250"
     CDS             1448..1699
                     /locus_tag="VF225_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144105.1"
                     /translation="MPRGYEEGPVAEEAQARRDALERGDRIEPLPHPPGRRAVYGSTD
                     EQQEKHFLGWADEVDPDELFESGRAWGITVLRERADQGR"
     gene            complement(1696..1980)
                     /locus_tag="VF225_02255"
     CDS             complement(1696..1980)
                     /locus_tag="VF225_02255"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX7144106.1"
                     /translation="MPTYILLSSLTAQGVQTLKSNPDRLREVNKDVEELGAKVLHQWA
                     TLGDYDFVNIVEAPDAATIARVSVSLGARGSTKIRTLTALTIDEFLTALG"
     gene            complement(1913..2665)
                     /locus_tag="VF225_02260"
     CDS             complement(1913..2665)
                     /locus_tag="VF225_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144107.1"
                     /translation="MPRPPLPAPLPPETRTVGQLVAEAVKLYGARFWPSLALGIGPAL
                     FGVADTELEGAARAVFDVGLGPLVFAASYGGAVALVRPIGRGRYVVVALAAGFVAFLP
                     ICLARLFRDFPGINLMAVAWLAFFCLAVPAALVERRGFVASLRRGVQLARADYVHVLG
                     SLATLLITIFLTGLVLFFSLRALSDQALRVAALLALLVLAPIFMLGAAILYVDQEARV
                     ESSPRPRRKSDADVHSAFEPDGAGRSDAEVEP"
     gene            complement(2676..3473)
                     /locus_tag="VF225_02265"
     CDS             complement(2676..3473)
                     /locus_tag="VF225_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144108.1"
                     /translation="MRRVWLRAAALAVVVVGAVAGLTVYFSTEKVPRCLVSGAKAWRA
                     PADGATHRYEVVILDRSACFFDMDRQQRLVGALDLAQAKWLTAAVPTESDTIRVEDAE
                     QGVAFVTRRGLLGVRALDLKTNRTLFVTHFKGFTWNPRFGPDPPSHGLSLAPDRPELW
                     VLDAPNSVVHVFDVSELPGRPPQPLEDIRLSKPISGDESPCTRACGRIGSLLHSADGR
                     FVYVGDAGDVIDTRTREIVANLEALHNSRVQLEVDWVGDKAVFPARS"
     gene            complement(3470..4009)
                     /locus_tag="VF225_02270"
     CDS             complement(3470..4009)
                     /locus_tag="VF225_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144109.1"
                     /translation="MILVVAATERELTAAVEAATLACGIGPVEAAASTARALAQGQRP
                     DALLHVGIAGARGIPVPQVVIGSEAVYEDALAAGLVPARVLPDPRLVDAAQRALPDAR
                     VLPIGTSARVGGTTDCEVEAMEGFAVLRAAKLAGVPAVEVRAVSNEIDEPDRDRWRFD
                     DALAALDAALPRLLSAFDG"
     gene            4351..4659
                     /locus_tag="VF225_02275"
     CDS             4351..4659
                     /locus_tag="VF225_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144110.1"
                     /translation="MYAMQIQTGELRETDLPPGYGEWSDYAKFAVTFAPRDRDLCSEA
                     ASDAFARWRRTGDVPRTLEELRACLWYEQRRWRFLGREPDTEGMRYAGALIRAMRHCL
                     "
     gene            complement(4656..6398)
                     /locus_tag="VF225_02280"
     CDS             complement(4656..6398)
                     /locus_tag="VF225_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7144111.1"
                     /translation="MIVVGAGGGGPVVAKELAARGLDVLLLEAGAAFEHSEREWSHQP
                     SEANNPATGIFRFGPGDRRLVPWARDLPQNSFVWQTAGVGGSTVHYFGNSPRAMPGVF
                     SDYAGADAAAYDRDHAVPFGYRELIPYYEWVEHTLPVQTAAMGTKEERFLRAAEKLGW
                     TAQTSKDISGASFRPQENAILQPAGTAGRTANRKQLVYPRARGCTFCGHCPQGCIEPL
                     NSPRNLRAKRSTHTSYVPMALSADRWTNGKAVTLVSDAFATRIETAGGAATGVTWRNV
                     HTGETTTEQARIVVLAAGAIESPRLWLNSGLPNPNDQVGRGLTDHYLDYIVGRVPAYT
                     GSSKGPASAARADFPGYGSIEQVGAAPALQAQSMGASDAGIAGFYDNGKGSRAGADAA
                     GRLVGERLKRFLGNVDRLLGVAVLTDDDLLPENRVTLSTVMSPDAHGPIARVSLPRRS
                     ARTLANREYLAARSVELVRAAGAREVVRLNWAPVLLHIHSTMRMGVGAADSVLDADAE
                     ARAVKRLFIADCSALPNSLGGPNPTLTMQALATRTAEKVFVRYFGGDPWVGHESPVSS
                     VDDTVTQAVMAAGL"
     gene            complement(6400..7023)
                     /locus_tag="VF225_02285"
     CDS             complement(6400..7023)
                     /locus_tag="VF225_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144112.1"
                     /translation="MTGVSRRELLERATALVAGAAVLDQLPHALRLQGWLDDAYAATP
                     NVVQQTMNGLVAFIVPGRDRYSRAQGTTTKTPGGIEAGTTPVLVRTLDRFLPGPLPLS
                     ATAATILNGFAAQVASVNRGFHPFSAPFANLSVVQKAQVFQAIESLQAESAGSIRFLV
                     GNLPDLVAFITYGRPLGKRLTKYAGVADGRKELKGYWQRRKAATPSA"
     gene            complement(7028..7645)
                     /locus_tag="VF225_02290"
     CDS             complement(7028..7645)
                     /locus_tag="VF225_02290"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7144113.1"
                     /translation="MGLYSAASRKLQDRFDTRRLADRIEERIVHDRIDDGDRAFIEAR
                     DMFFIATTDDEGQPQCSYKGGEPGFVRVLDDTTIAFPIYDGNGMYLTAGNLVATKNVG
                     LLFIDFEGRKRMRLNGVASVDDADPLLPEYPEAQLVVRVHATEVFPNCPRYIHEYKLV
                     KRSRFVPKAECETPVPQWKQSDWAHDVLPENDPANDPSREVIPRG"
     gene            7762..8157
                     /locus_tag="VF225_02295"
     CDS             7762..8157
                     /locus_tag="VF225_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144114.1"
                     /translation="MGYHVADGSKLEWEERPPAAEGLEPRLTADVTTAAELKQSRGRI
                     WRYPAGTRGRRHADKAQEEVFVVISGTLTMLLGEPPERVDVGPRSVVAVEPGTPLQAR
                     NESDEELVLYIYGAPPEQAGADFFDDVEL"
     gene            complement(8154..10625)
                     /gene="clpB"
                     /locus_tag="VF225_02300"
     CDS             complement(8154..10625)
                     /gene="clpB"
                     /locus_tag="VF225_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX7144115.1"
                     /translation="MDFNKLTIKSQEAVAAAQELARRMGNPELYPEHLLLALLDQELP
                     QQLVPDAAELRAQAEAALSAKPSVQGMQQQPNVSAAFSRVLDKAADEAKRMEDDYVST
                     EHLLLALDVVPRDQLEAKIKAVRGGQRVTSQDPEETYQALEKFGRDLTAAAEEGKLDP
                     VIGRDEEVRRVIQVLSRRTKNNPVLIGDPGVGKTAIVEGLAQRIVAGDVPEGLKGKRV
                     WALDIGALLAGSKYRGEFEERLKAVLNEIQSAEGRIILFIDELHTIVGAGAAEGAVSA
                     GNLLKPMLARGELRAVGATTLDEYRKHIEKDAALERRFQPIFVGEPSVADTIAILRGL
                     KERYEAHHGVRIRDAALVAAAVLSDRYITDRFLPDKAIDLVDEAASRLRMEIDSSPVE
                     LDEADRRVRQLEIELAAMQKESKDVREPLERELAEAKERHTELAARWSHEKEALDRVK
                     EITQRIDELRSEYERAERAGDFNRAAEIRHGELPQLERELAERDGSQPENAMVKEEVD
                     EDDVAAVVARWTGIPVDRLLEGETEKLIHMEDRLHQRVVGQDEAVEAVANALRRARTG
                     LQDPNRPIGSFVFLGPTGVGKTELARALAEFMFDDERALVRLDMSEYQERHTVARLIG
                     APPGYVGYEEGGQLTEAVRRRPYSVILLDEIEKAHNEVFDVLLQILDDGRLTDGQGRT
                     VDFRNTVLIMTSNLRSQEQMREVFRPEFLNRIDEIVEFAPLTKEQLADIVELQLERLR
                     VRLADRGIELELTDEAKHVLAEAGWDPTYGARPLKRAIQRMVENPLALRLLEGEFGEG
                     DAVRVDAQEGQLVFERAPAREPAVV"
     gene            complement(10762..11130)
                     /locus_tag="VF225_02305"
     CDS             complement(10762..11130)
                     /locus_tag="VF225_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7144116.1"
                     /translation="MDDRPRYMISVAADLVGMHPQTLRIYESKGLIRPKRTAGNTRLY
                     SEVDIERLRLIQQLTNDLGLNLAGVEQVLELQDEVQRMRRTLDRMEREMREAVNQVHR
                     QYRRDLVPWKPPIDLIPTRH"
     gene            complement(11240..>12068)
                     /locus_tag="VF225_02310"
     CDS             complement(11240..>12068)
                     /locus_tag="VF225_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX7144117.1"
                     /translation="NDLGDLGDLFGGIFNRGQAGRARRPQPERGADIEIPVSLSFEDS
                     LRGLETKIPVEVTTACRECGGTGAEPGTSPVICPECNGRGVISESQGLFALSQPCPRC
                     RGNGTVIEKPCKKCKGSGRERRTRRYTVKIPAGVKDGTRIRLKGKGEIGEGGGPAGDL
                     YVVTRVAPSKKFERRGADLVIEVPVTYTEAALGATVEVPTPYGERVSLKVPAGTQDGR
                     QLRIRGHGAPKLDGSGKGDLIARLRVSVPKKLSKKEREALEELQKLSRDDPREALFS"
BASE COUNT         1934 a         4186 c         4044 g         1904 t
ORIGIN      
        1 agcccggcga cgagacccgc gccgaagagc atgaagatcg caatcccgtt cgcgatcgag
       61 ctcagaaaca ccgaaccgag gagcgacaac gcggccacga tcagaacgcc cgcggcgagc
      121 tcgagccccg gcgtcacgat ccggtccggc caccaggagc cttcgagacc cgtgagcacc
      181 atcgcggtga agtagaggat caggacgtac gggacacaga ccgccccggc gccaaggaaa
      241 cgcgcgacga gcaacgtcga ccggccgatc gggcgaacta cgagcggctg gaggagcgcg
      301 cgctccgcat cgccgcggac ggttccgagg gtgaggaaga tggccaggac gacgccgaga
      361 aagagcgtcc cgaacatcgc catgccgaac aggaacgcgc cggcgaacgt ccgcgcatcg
      421 acaccctcgg gcggcgccac gtttgcgacg tggtggaacg catagcgcgc cccgagccag
      481 tacaaggcga ggaacgcaat cgtcaggaag cagacgacga cgaacacctt gcggcgtagc
      541 gcctcgcgca gtccgtaacc ggcgatcgtc cagacagctg tcacgagccc tcgccgccga
      601 cggcctcgac ataaacgtct tcgagcgtcg atgtcagcag actcgcgccg tacacgctct
      661 tcccgtcgcg aacgagctcg gcgacgagac gaggcatgtc gtcgcgcccc gcctccgggt
      721 atgtgcgctt gcctgcgtcc gtctcgatct caacgccgcg cggcctcgcc aactcggccg
      781 gcgtcccagc ggtcacgacg cgtccaccga ggaggatgac gacgcggtcg cagacgagct
      841 cgatctcact gagcaggtgc gagttcagca ggacggaaac gccgcgccgc tgcagctctt
      901 cgagcaacgt gcgcaccgtc cgcctgccga ccggatcaag cgcgctcgtc ggctcgtcga
      961 gaacgagcag tcgcggcgag ccgacgagcg cctgcgcaat cccgaggcgc tgctgcatcc
     1021 ccttcgacat cgcttcgaca ggccgcctcc gtgcgtcgtg caagctgacg agctcgagca
     1081 actcgacacg ctcgcgttca ccgccgcgcg agccggccag gcgctggtgc agctcgagca
     1141 actcgtcggc agtcatccag ccgggaaagc ggaaaagctc cgcgagatag ccgagcgcag
     1201 cccgcgcctc ccgcgagcct gcacgagcgc cgcacacttc cacgcgccca gcagtcggac
     1261 ggacgaggcc gcacgcgatc ttgacgagcg tggacttccc ggcgccgttc ggtccgagca
     1321 ggccgacgag ctcgctgtct ccgacctcga ggtccacgcc gccgagtgct tcgacggcgc
     1381 cgtagcgctt cacgagaccg ctgacggcga gcgctgcgga cacgacggcg agcgtacact
     1441 cactcgtgtg ccacgcggtt acgaggaagg accggtcgcc gaggaggcgc aggcgcgccg
     1501 cgacgcactc gagcgtggcg accggatcga gcctctaccc catcccccgg gacggcgcgc
     1561 ggtctacggc tcgacggacg aacagcagga gaagcacttc ctcggctggg cggacgaggt
     1621 cgatccggac gagctgttcg agtccgggcg cgcctggggt atcaccgtgc tccgcgagcg
     1681 cgcggaccag gggcgctagc cgagcgccgt caggaactcg tcgatcgtca gcgcggtgag
     1741 cgtgcggatc ttcgtgctgc cgcgcgcacc gagcgaaaca gagacgcggg cgatcgtcgc
     1801 cgcgtccggc gcctcgacga tgttcacgaa gtcgtagtcg ccgagcgtcg cccactggtg
     1861 gaggaccttg gcgccgagct cctcgacgtc cttgttgacc tcgcggagac ggtcagggtt
     1921 cgacttcagc gtctgaacgc cctgcgccgt caggctcgaa agcagaatgt acgtcggcat
     1981 cgctcttcct cctcggtcgg gggctcgact ctactcgggc ttcctggtcg acgtacagga
     2041 tcgcggcacc cagcatgaag atcggcgcaa gcacgagcag cgccaacaag gccgctacgc
     2101 gcagtgcctg gtcgctcagc gcgcgcagcg agaagaacag cacgagaccg gtgaggaaga
     2161 tggtgatgag gagcgttgcc agcgagccga gcacgtggac gtagtccgca cgagcgagct
     2221 gcacacctcg ccgcaacgaa gcgacgaagc cgcggcgctc gacgagggcc gcaggcacgg
     2281 ccagacagaa gaatgcgagc caggcgaccg ccatgagatt gatccccgga aaatcccgga
     2341 agagccgcgc caggcagatc ggtaggaatg cgacgaagcc ggccgcgagc gcgacgacca
     2401 cgtagcgacc tcgcccgatc ggcctgacca gcgcgacagc gccgccgtac gaggcggcga
     2461 agaccagggg cccgaggcca acgtcgaaga ccgcccgcgc cgccccctcc agctctgtgt
     2521 ccgccacacc gaacagcgcc ggcccgatgc cgagcgccag tgagggccaa aagcgtgcgc
     2581 cgtaaagctt caccgcttcg gcgacgagct gcccgacggt gcgggtctcg ggtggaagcg
     2641 gcgccgggag cggtggacgg ggcacgcgtt cgaatctagc tacgcgcggg gaagaccgcc
     2701 ttgtcgccga cccagtccac ttccagctgc acccgtgaat tgtggagcgc ttccaagttg
     2761 gccacgatct cgcgcgtacg cgtgtcgatc acgtcgccgg catcgccgac gtagacgaag
     2821 cgaccgtccg cactgtgcag gagcgagccg atgcgaccgc acgcacgcgt gcacgggctc
     2881 tcgtcgccgg agatcggctt cgagaggcgg atgtcctcca gtggctgcgg cggccggccg
     2941 gggagctcgc tcacgtcgaa gacgtgcacg acgctgttcg gcgcgtcgag cacccagagc
     3001 tcggggcggt ccggagcgag cgagagccca tggctcggcg ggtccggccc gaagcgcggg
     3061 ttccaggtga aacccttgaa gtgcgtgacg aaaagcgtgc ggttcgtttt gaggtcgaga
     3121 gcgcgaaccc cgagcaggcc acgccgcgtc acgaacgcga ctccctgctc cgcgtcctcg
     3181 acacggatcg tgtcggattc ggtaggcact gcggcagtga gccacttcgc ctgcgcgaga
     3241 tccagcgccc cgaccagacg ctgctgccga tccatgtcga agaagcacgc ggagcgatcg
     3301 aggatcacga cctcgtagcg gtgtgtcgcc ccatctgcag gcgcgcgcca ggctttcgct
     3361 cccgacacga ggcagcgcgg aaccttctcg gtgctgaagt acacggtcag accggcgacg
     3421 gcaccgacga cgacaaccgc gagcgcggcc gctcgcagcc acacccgcct catccgtcga
     3481 acgctgagag cagcctcggc agagcggcgt cgagggcggc gagcgcgtcg tcgaagcgcc
     3541 agcggtctcg atccggctca tcgatctcat tggagacggc gcgcacctcg actgcaggca
     3601 cgccggcaag cttcgccgcc cgcaacaccg cgaagccttc catcgcctcg acctcgcagt
     3661 cagtcgtccc gccgacgcgc gcgctcgtgc cgatcggcag gacgcgcgcg tcgggcaggg
     3721 ctcgctgcgc cgcgtcgacg aggcgcggat ccgggagcac gcgcgccggc acgaggccgg
     3781 ctgcgagtgc gtcttcgtag actgcctccg agccgatcac gacctgtggc accggaatcc
     3841 ctcgcgcacc cgcgatgccc acgtgaagca acgcgtcggg ccgctgccct tgcgcgagag
     3901 cgcgtgccgt cgaggcggcc gcctcgaccg ggccgatccc gcaggcgagc gtcgccgctt
     3961 caacggcagc tgtgagctcg cgctcggttg cggcgacgac gaggatcatt ccggccaacc
     4021 gtagccgtgt caccccaaag ggtgaacctg tgcaaggttc gccgggcgac ggcatctcag
     4081 tccgtagccg caactcttcg ccgccgcagg ccgctaagcg gcgatgacaa tcagagagcc
     4141 gtccaccgca ggggcggctc ttgcctttca cagccgggtc accctgaagg gggatttctc
     4201 gttaccgggg caagatccgg cgacttcgag cgtctatgag tgaaaggaac aacaaggaag
     4261 tcgccccgga ggcccgccgc aggccgaagg ggcaaaccat tcccacaaag ggccgcggca
     4321 gcggcccttt gcgtatttcg gaggtatgag atgtacgcga tgcagatcca aacgggggag
     4381 ctgcgggaga ccgacctacc gcccggttac ggcgagtgga gcgactacgc caagttcgcc
     4441 gtgacattcg ccccacggga ccgggacctg tgttccgaag cagccagcga cgccttcgcg
     4501 cgctggcggc gcacgggcga tgtcccgcga acgctggaag agctccgcgc ctgcctctgg
     4561 tacgagcagc gccggtggcg cttcctcggc cgcgagcccg acaccgaggg catgcgctac
     4621 gcaggggcgc tcatccgcgc catgcgccac tgcctctaga ggccggccgc catcaccgcc
     4681 tgggtcaccg tgtcgtcgac cgacgacacc ggtgactcgt gccctaccca cgggtcgccg
     4741 ccgaagtagc ggacgaagac cttctccgcg gttcgcgtcg ccagcgcttg catcgtgagc
     4801 gtcgggttcg gcccgccaag gctgttcggc agggccgagc aatcggcgat gaagagacgc
     4861 ttgaccgcgc gcgcttcggc gtcggcatcg agcaccgagt cggcggcgcc gacgcccatc
     4921 cgcatcgtgc tgtggatgtg aagcagcacg ggcgcccagt tgagcctgac gacttcgcgc
     4981 gcacccgcgg cgcggacaag ctccaccgag cgcgccgcga ggtactcccg attcgcgagt
     5041 gtgcgggcgc ttcgccgcgg cagactgacc cgcgcgatcg gcccatgggc gtccgggctc
     5101 atcaccgtgg agagcgtgac gcgattttcc ggcaggaggt cgtcgtccgt caggaccgcg
     5161 actccgagca ggcggtcgac gttgccgagg aaccgcttga gccgctcgcc gacgagacgt
     5221 ccggcggcgt cggctccggc acgcgacccc ttgccgttgt cgtagaagcc ggcgatgccg
     5281 gcgtcgctcg cacccatgga ctgcgcctgc agcgccggtg cggcgccgac ctgctcgatc
     5341 gagccataac ccggaaagtc cgcgcgtgcc gcggatgccg ggcccttgct tgacccggtg
     5401 tacgcgggca cccgcccgac gatgtagtcg aggtagtgat cggtgaggcc gcgcccgacc
     5461 tggtcgttcg ggttgggcag gccgctgttg agccacagtc ggggcgactc gatcgcaccc
     5521 gcggcgagga cgacgatcct cgcctgctca gtcgtcgtct cgccagtgtg gacgttccgc
     5581 caggtcacac ctgtggcagc gccgcccgct gtctcgatcc tcgttgcgaa cgcgtccgac
     5641 acaagcgtca cggccttgcc gttcgtccac cgatccgcgc taagcgccat cggcacgtag
     5701 ctggtgtgtg tcgagcgttt cgcgcggagg ttgcgaggcg aattgagcgg ctcgatgcaa
     5761 ccctgcggac agtgcccgca gaaggtgcaa ccgcgtgcac gcggatagac gagctgcttg
     5821 cgattcgccg tacgccctgc ggttccagcc ggttggagga tcgcgttctc ctgcggccgg
     5881 aaggaggctc cgctgatgtc cttcgacgtc tgcgccgtcc agccgagctt ctccgcagcg
     5941 cgcagaaagc gctcttcctt cgttcccatc gcggccgtct gcacgggcag cgtgtgctcg
     6001 acccactcgt aataagggat caactcgcgg taaccgaacg gcactgcgtg gtcgcggtcg
     6061 tacgcggcgg catcggcacc cgcgtagtcg gaaaacactc caggcatcgc gcgcggcgag
     6121 ttgccgaagt agtggacggt ggagccgccg acgccggcgg tctgccacac gaaggagttc
     6181 tgcggcaggt cgcgcgccca cggtacaagc ctccggtcac ctggaccgaa acggaagatc
     6241 cccgtcgccg ggttgttcgc ctcgctcggt tgatgcgacc attcgcgctc actgtgctcg
     6301 aaagctgctc cggcttcgag cagcagcacg tcgaggccgc gcgcagccag ctctttggcg
     6361 acgaccggcc caccgccgcc tgcgccaacg acgatcacgt cacgcactcg gagttgcggc
     6421 ctttctgcgt tgccagtagc ccttcagttc cttgcgcccg tcggcaaccc cggcgtactt
     6481 cgtcagccgt ttcccgagcg ggcgcccgta cgtgatgaac gcgaccaggt cgggcaggtt
     6541 gccgacgaga aatcgaatcg accctgcgct ctcggcttgc aggctctcga tcgcctggaa
     6601 gacctgggcc ttctgcacga ccgagaggtt tgcgaagggt gctgagaacg ggtggaaccc
     6661 gcgatttacc gacgccacct gcgcggcgaa cccgttcagg atcgtcgcgg cggtcgccga
     6721 cagcggcaac ggccccggca ggaaccggtc gagcgttcgg accaggacgg gcgtggtccc
     6781 cgcctcgatc ccgccgggtg ttttcgtcgt cgttccctgc gcgcgcgagt agcggtcccg
     6841 tccgggaacg atgaaggcga cgagcccgtt catcgtctgc tggacgacgt tcggcgtcgc
     6901 ggcgtacgca tcatcgagcc agccctggag tcgcagcgcg tgcggcaact ggtcgagcac
     6961 cgcggcgccc gccacgagtg ccgtcgcccg ttcgagcagc tcgcgccgtg agacccctgt
     7021 cactgcctca cccgcggggg atgacctccc gtgacggatc gttggccgga tcgttctcgg
     7081 gcagcacgtc gtgggcccag tcgctctgct tccactgcgg cacgggcgtc tcgcactcgg
     7141 ccttgggcac gaagcgggac cgtttgacca gcttgtactc gtggatgtag cgcgggcagt
     7201 tgggaaagac ctcggtcgcg tgcacgcgca cgacgagttg cgcttcgggg tactcgggca
     7261 gcagagggtc ggcgtcgtcg acggaggcga caccgttcag gcgcatccgc ttgcggccct
     7321 cgaagtcgat gaagagcagg ccgacattct tcgtcgccac gagattcccg gccgtcaggt
     7381 acatgccgtt gccgtcatag atggggaacg cgatcgtcgt gtcgtcgagc acacggacga
     7441 acccgggctc accgcccttg tacgagcact gcggctggcc ctcgtcgtcg gtcgtcgcga
     7501 tgaagaacat gtcgcgcgcc tcgatgaacg cacggtcgcc gtcgtcgatg cggtcgtgaa
     7561 cgatccgctc ctcgatccgg tcggcgaggc gcctcgtgtc gaagcggtcc tggagcttgc
     7621 ggctggcggc gctgtagaga cccacatccc cgattttctc agggaaacct catgagcacc
     7681 aaacaatcca tgtccatgat gggttcagcc gcaagacgga gggccgctgt ggggagcggc
     7741 cctttttttt atgctccagt cgtgggctac cacgtcgccg acggttcgaa gctggaatgg
     7801 gaagagcggc cgcccgcggc cgaggggctc gagccccggc tgacggccga cgtcactacc
     7861 gccgcggagc tgaagcagtc ccgtggtcga atctggcgct atcccgcggg cacgcgcgga
     7921 cggcgccatg ccgacaaggc acaggaggag gttttcgtcg tcatctcggg gaccctgacg
     7981 atgttgctcg gcgaaccgcc cgaacgggtc gacgtgggcc cgcgaagcgt cgtcgccgtc
     8041 gagccgggga cgccactgca ggcgcgaaac gagagcgatg aggagcttgt cctctacatc
     8101 tacggcgctc cgcccgagca ggccggcgcc gacttcttcg acgacgtcga gctctagacg
     8161 acggcgggct cgcgcgcggg cgcgcgctcg aacacgagct ggccctcctg cgcgtcaacg
     8221 cgaaccgcgt cgccttcgcc gaactcgcct tcgaggagcc ggagcgccaa ggggttctcg
     8281 accattcgct ggatcgcacg cttcaggggc ctcgcgccgt atgttggatc ccaaccggcc
     8341 tcggccagca cgtgcttcgc ctcgtcggtg agctcgagct cgatgcctcg gtcggcgagc
     8401 cgcacgcgga gccgttcgag ctgcagctcg acgatgtccg cgagctgctc cttcgtcagc
     8461 ggcgcgaact ccacgatctc gtcgatcctg ttcaggaact ccggccggaa gacctcgcgc
     8521 atctgctcct gcgagcgcag gttcgaggtc atgatcagca cggtgttgcg aaagtcgacg
     8581 gtgcggccct ggccgtcggt gaggcggccg tcgtcgagga tctgcagcag gacgtcgaag
     8641 acctcgttgt gcgccttctc gatctcgtcg aggaggatca ccgagtacgg ccggcggcgc
     8701 acggcttccg tgagctgacc gccttcctcg tagccgacgt agccgggcgg ggcgccgatc
     8761 agccgcgcga cggtgtgccg ctcctggtac tccgacatgt cgagccgcac cagggcgcgc
     8821 tcgtcgtcga acatgaactc cgccagtgcg cgtgcgagct cggtcttgcc cacgccggtc
     8881 ggcccgagga agacgaagga gccgatcggg cgattcggat cctgtagccc tgtgcgtgca
     8941 cgtctgagtg cgttggcgac tgcttcgacg gcctcgtcct ggccgacgac gcgctggtgc
     9001 aggcggtcct ccatatggat cagcttctcc gtctccccct cgagcagcct gtcgaccggg
     9061 atcccggtcc agcgcgcgac gaccgctgcg acgtcgtcct catcgacctc ttccttcacc
     9121 atcgcgtttt cgggctgcga accgtcgcgt tcggcgagct cgcgttccaa ttggggaagc
     9181 tcgccgtgcc gtatctcggc tgcgcggttg aagtcgccgg cgcgttccgc gcgctcgtac
     9241 tcggaacgca gctcgtcgat ccgctgcgtg atctccttga cccggtcgag cgcctccttc
     9301 tcgtggctcc agcgggcggc gagctccgtg tgccgttcct tcgcctcggc gagctcgcgc
     9361 tcgagcggct cacgaacgtc cttcgactcc ttttgcattg cggccaactc gatctcgagc
     9421 tggcgtacgc ggcgatcggc ttcgtcaagc tcgaccgggc tcgagtcgat ctccatgcgg
     9481 aggcgcgacg ccgcctcgtc gacgaggtcg atcgccttat cggggaggaa acggtcggtg
     9541 atgtagcggt cggagagcac cgcggcggct acgagcgccg cgtcgcgaat gcgcacgccg
     9601 tgatgcgcct cgtagcgctc cttcaggccg cgcaggatcg cgatcgtgtc cgcgaccgac
     9661 ggctcgccga cgaagatcgg ctggaagcgc cgctcgaggg cagcatcctt ctcgatgtgc
     9721 ttccggtact cgtcgagcgt agtcgcgccg acggcgcgca gttcgccacg cgcgagcatc
     9781 ggcttgagca gattgccggc ggagacggcc ccttccgcgg cgccggcgcc gacgatcgtg
     9841 tgcagctcgt cgatgaagag gatgatccgg ccctcggccg actggatctc gttgagcacg
     9901 gccttgaggc gctcctcgaa ctcgccgcgg tacttcgagc ctgcgagcag cgcgccgatg
     9961 tccagcgccc aaacgcgctt ccccttgaga ccttccggca cgtcgccggc gacgatgcgc
    10021 tgcgcgagcc cttcgacgat cgcggtcttg ccgacgcccg gatcaccgat gaggacgggg
    10081 ttgttcttcg tccggcgcga gaggacctgg atcacgcggc gcacttcctc gtctcggccg
    10141 atgacagggt cgagcttccc ttcctccgcg gccgccgtga ggtcgcggcc gaacttctcg
    10201 agtgcctggt aggtctcctc cggatcctgc gaggtcacgc gctggcctcc tcgtacggcc
    10261 ttgatcttcg cctcgagctg gtcgcgcgga acgacgtcga gggcgagcaa aagatgctcg
    10321 gtcgagacgt agtcgtcctc catccgcttt gcctcgtcgg cggccttgtc gagcacgcgc
    10381 gagaacgcgg cggagacgtt cggctgttgc tgcattccct gcacggacgg cttcgcactc
    10441 agcgcagcct cggcctgcgc gcgcaactcg gcagcgtcgg gcacgagctg ctgcggcaac
    10501 tcctggtcaa gcagcgcgag cagcaaatgc tctgggtaga gctcggggtt ccccatccgg
    10561 cgcgcaagct cctgcgcagc cgcgacggcc tcctggctct tgatcgtcag cttgttgaag
    10621 tccatacctc gtgcgcctct cgtttcagtt tcgtacccgt gcctggcacc ggtacgtccg
    10681 cctgcggatc caggcggttc gcagatctcg tcgcagtggc gtcacggttt cgttcccgtg
    10741 cctggcaccg gtacgtgtcc attagtgccg cgtcgggatc aggtcgatgg gcggcttcca
    10801 cgggaccagg tcgcggcgat actggcgatg cacctggttg accgcctcgc gcatctcgcg
    10861 ctccatgcgg tccagcgtcc gccgcattcg ctgcacctcg tcctgcagct ccagcacctg
    10921 ctcgacgccc gccagattga ggccgaggtc gttcgtcagc tgctggatca gccgcagacg
    10981 ctcgatgtcc acctcggagt acaagcgcgt gttcccggcc gtgcgtttcg ggcggatgag
    11041 gcccttgctc tcgtagatcc gcagcgtctg cggatgcatg ccgacgaggt ccgcggcgac
    11101 cgagatcatg taacgcggac ggtcgtccat cactcgctct ccttcagccg ccgcagcgcc
    11161 tcgagcttgc ggcgcatgat cacgaacgtc acgaggacgc cgaccgtgcc gacactgacg
    11221 aggacgatcg ccgcagcttt cacgagaaca acgcctcccg cggatcgtcg cgcgacagct
    11281 tctgcaattc ctcgagcgcc tcgcgctcct tcttcgagag cttcttcggg acgctcacgc
    11341 gcaggcgcgc gatcaggtcg cccttgccgc tcccgtcgag cttgggagcg ccgtggccgc
    11401 ggatgcgcaa ctggcgtccg tcctgcgttc ccgcgggaac cttcagcgat acgcgctcgc
    11461 cgtatggggt cggcacctcg accgtcgccc cgagcgcggc ctccgtgtac gtgaccggaa
    11521 cctcgatgac gagatccgcg ccgcggcgtt cgaacttctt cgacggcgcg acgcgcgtga
    11581 cgacatagag atcgcccgca ggcccacccc cttcgccgat ctcacctttg cccttcagcc
    11641 ggatccgcgt gccgtctttg actcccgccg ggatcttgac ggtgtagcga cgggtacgcc
    11701 gctcgcggcc ggagcccttg cacttcttgc acggcttctc gatgacggtg ccgtttccgc
    11761 gacagcgggg acacggctgc gacagggcga acagtccctg gctctccgag atcacgccgc
    11821 ggccgttgca ctcgggacag atcacgggag acgtccccgg ctcggcgccc gtgccgccgc
    11881 actcgcggca cgcggtcgtc acctcgaccg ggatcttcgt ctcgagcccg cggagggaat
    11941 cctcgaacga caggctgacc ggaatctcga tgtcggcgcc gcgctcgggc tggggccgtc
    12001 gcgcacgccc ggcctggccg cggttgaaga ttccaccaaa caggtcgccg agatcgccga
    12061 ggtcgttg
//
LOCUS       DASVEU010000057         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_428224,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000057 DASVEU010000000
VERSION     DASVEU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428224"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1467
                     /locus_tag="VF225_02315"
     CDS             367..1467
                     /locus_tag="VF225_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144118.1"
                     /translation="MSAPASFGPIQYDGTPPSVAVALSRGPDAGGWYNHPVDFQANGS
                     DNLSGIASCNSGSFSGGGSVNASCSDRAGNVGGAGVTVNYDGSAPSIDSITFDRQPDA
                     NGWYNHPVRVTFHGSDGGSGIGACSDVTYAGPDTTSTTVNGSCTDNAGNRAGGTSPAL
                     KYDATPPTLSNLGFDWDDGTATLTWTASPDTKAIEIDRTPGTAGPDPSAVFKGLASSF
                     EDTGLTNKVKYVYTINGFDEAGNKASESVSIVPGAKLYSPARGSVVTSLPLLAWRPVA
                     GASYYNLQVYYGVGKALRRLASLTVSGRKVMSVWPLQPRYRMKKTWRYKGKARKLAVG
                     HYRWYVYPGIGKRTAHKYGPLIGQSDFFVAKR"
     gene            complement(1649..>3869)
                     /locus_tag="VF225_02320"
     CDS             complement(1649..>3869)
                     /locus_tag="VF225_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7144119.1"
                     /translation="QAGRLAIGPWQTLMDEFLVDGETTLRNLEAGLARAADFGSAMRV
                     GYLPDMFGHVAQMPQILRLAGIETAVVWRGVPAAVDFHRFVWEGPDGSSVVTEYLPSG
                     YGNAAYLFEVPGPADLAPLEERFSPWFRGDPVLGMVGTDHMPLPRELPERVPAGATIG
                     TLADYLTGASPEGLSHWHGEMRSAARANLLPGVVSARIDLKAACGRAERWLERYAEPL
                     QTLYGGAWPEPFLAQAWMRMFQNSAHDSICGCSADEVSAQVLVRYAEAEQIGRELAQR
                     AVARIAADVAGDAFVIVNPSPRERSDLVELELVVREEWESVELELPDGSRVPTQETHR
                     QEPLLWEAKLVGADVPLVIARRLHGRELFGRYVNGFRIEDGRATLEVGDEPDPESLDI
                     AQLVQELTLATAEGEWTLRVVARSKRTLVAAVTAPPLGWISVRPVVAQGTVTGTLSAI
                     DVSQLTRIVRGGDVGDSYNYAPPPGDVLVDAPIDEGLELIEDGPLRRVEVVHRTYMWD
                     GERVETQTRFEQRAEEPFVRIRIDFDNPCDDQRVRVHVPLRERADHSHAEGQFAIVER
                     GLEPEGGYGEVPIPTYPASSFVAAGGIALLLDHVTEYEVAGDELALTVLRSTGLISRT
                     QHPWREEPAGPSLPIPAAQLRGPRSFSFAYLPGAEGILEHAERYRHPFLTTQGTAPAG
                     DLRSHAGPVLDGDPSVVLTAFQPGRARIVNESSDPQSVRFAGQQLDLRPWEIRTISI"
BASE COUNT          694 a         1317 c         1260 g          598 t
ORIGIN      
        1 aacgtcgtag tcgctttttt cgtttcgccc gatccaacga tcgtcggtac gcctggctgt
       61 ggtgtgttct cggtcgcctc cgacacatcc ggacaatcct tccaatgccg cgtcgactac
      121 aacaccggcg gctcttatag cactccagta ataacggtga agaaggacgc cactcctcca
      181 acagtgactg gcggctctcc gagccgcggt cccgattcga acggctggta caaccacccg
      241 gtcgatatca acttcaacgg gtccgacgca acatcgggtc tcgctggctg caactcaact
      301 acgtacggcg gcccggacag cggcagcgcc tcgttctccg ggacctgcac tgacaacgca
      361 ggaaacgtga gcgcgccggc cagcttcggg ccaatccagt acgacgggac gcccccgtcc
      421 gtagcagtag ccctgtcgag aggccctgac gccggtggct ggtacaacca tcccgtcgac
      481 tttcaggcga acgggagcga caacctgtcg ggaatcgcgt cctgcaactc cgggagcttc
      541 agcggcggcg gttcggtcaa tgccagttgc agtgacagag ccggcaacgt cggcggagcc
      601 ggcgtaaccg tgaactacga cgggagcgcg ccctctatcg acagcatcac cttcgatcgt
      661 caacctgacg ccaacggctg gtacaaccac cccgtccggg tgacgttcca cggcagcgac
      721 ggcggctccg gcatcggcgc gtgcagcgac gtcacgtacg ccgggcccga caccacgagc
      781 acgaccgtca acgggagctg cacggacaac gcgggcaacc gagccggcgg cacgtcgccg
      841 gcactcaagt acgacgccac cccgcctacc ctcagcaacc tcgggttcga ctgggatgac
      901 ggaaccgcga ctttgacctg gacggcgtcg ccagatacga aagcgatcga gatcgaccga
      961 acgccgggaa cagccggtcc agatccgtcc gcagtcttca aaggcctcgc ctcgagcttc
     1021 gaggacacgg gcctgacgaa caaggtcaag tacgtgtaca cgatcaacgg tttcgacgag
     1081 gccggcaaca aggcgtcgga aagcgtcagc atcgtgccgg gagcgaagct gtattcgccg
     1141 gcgcgagggt cggtcgtcac cagtcttccg ctgctggcct ggcgcccggt ggcgggagcc
     1201 tcctattaca acctgcaggt ctattacggc gtcggaaagg cgctacggcg cttggcctcg
     1261 ctcaccgtga gcgggcggaa ggtgatgagc gtgtggccgc tgcagccgcg ctaccgcatg
     1321 aagaagacct ggcggtacaa gggcaaggcc cgcaagctcg cggtaggtca ttaccgctgg
     1381 tacgtctatc cgggcatcgg caagcgaacc gcgcacaagt acggcccgct gatcggacaa
     1441 agcgacttct tcgtcgcgaa gcgctagttc gcgagcattc cgttcgtccg gccactgtgg
     1501 cctcttccgt ttccaactcc accggacgca agggccgcaa cagccaagac gagccacgcc
     1561 acaaggcaca ggatcaccag ccgcattgac acttctgacg tctgccgacc tttgcagttg
     1621 gcgtacgtgg tattaagccg ttacgagttc aaatcgagat ggttcggatc tcccagggac
     1681 ggagatcgag ctgctgtccg gcgaagcgga ccgactgcgg gtccgagctc tcgttgacta
     1741 tccgtgcgcg tcccggctgg aaggcggtca gcaccacgct cgggtcgccg tcgaggacgg
     1801 gaccggcgtg cgaacgaagg tctcctgcag gcgccgttcc ctgcgtcgtc aggaaaggat
     1861 gacgatagcg ctcggcgtgc tcgaggattc cctcggctcc cgggaggtat gcgaacgaga
     1921 agctgcgcgg ccctcgcagc tgggcggccg gaatcggtag tgatgggccc gcaggctcct
     1981 cccgccacgg gtgttgggtc cggctgatga gcccagtcga acgaagcacc gtcagggcga
     2041 gctcgtcgcc ggcgacctcg tactccgtga catggtccag cagcagcgca atgcctccgg
     2101 ccgcgacgaa cgaggatgcg gggtaggtcg ggatcggcac ctcgccgtaa ccgccctccg
     2161 gctcgaggcc gcgctcgacg atcgcgaact ggccctcggc gtgcgagtgg tcggcccgct
     2221 cccgcagtgg aacatgcacg cgtacgcgtt ggtcatcgca aggattgtcg aaatcgatac
     2281 gaatgcggac gaacggctcc tcggcgcgtt gctcgaaccg cgtctgcgtt tcgacccgct
     2341 ccccgtccca catgtaggtg cgatggacga cctcgacgcg gcggagcggt ccgtcctcga
     2401 tcaactcgag cccttcgtcg atcggcgcgt cgacgagaac gtcaccgggt ggcggcgcgt
     2461 agttgtaact gtcccccacg tcgccgccgc gaacgatccg tgtcagctgc gatacgtcaa
     2521 ttgcggacaa cgtccccgtg acagtccctt gggcaactac cgggcggaca ctgatccagc
     2581 cgagcggcgg tgcggtcacc gcggcgacga gcgtgcgctt cgaccgtgcg acgacgcgca
     2641 aggtccactc gccttcggcg gttgcgagcg tgagctcttg gacgagctgc gcgatgtcga
     2701 gcgactcggg gtccggctcg tcgccgacct ccagcgtcgc tcggccgtcc tcgatgcgaa
     2761 agccgttcac gtaccggccg aagagctcgc gaccgtggag acggcgcgcg atcacgagcg
     2821 gaacgtccgc gccgacgagc ttcgcctccc acaacagcgg ctcctgacgg tgggtctcct
     2881 gcgtcgggac gcgcgagccg tcggggagct ccagttccac cgactcccat tcctcgcgaa
     2941 cgacgagctc gagctccacg aggtcggagc gttcgcgcgg tgacgggttc acgatcacga
     3001 aggcgtcgcc ggcaacatca gcggcaatcc gtgcgacggc acgttgcgcg agctcgcgcc
     3061 cgatctgctc ggcctccgcg tagcgcacga ggacctgcgc cgaaacctcg tccgcggagc
     3121 agccgcagat cgagtcgtgc gcggaattct ggaacatgcg catccacgcc tgcgcgagaa
     3181 acggctcggg ccaggcgcct ccgtacaacg tctgcagcgg ctcggcgtag cgttcgagcc
     3241 accgctcggc gcgcccgcat gccgctttca gatcgatgcg cgcggagacg acgcccggga
     3301 gcaggttcgc ccgcgcagcg gagcgcatct cgccgtgcca gtgcgacagg ccttcgggag
     3361 acgcgccggt gaggtagtcg gcgagcgtcc cgatcgtcgc gccggccggg acgcgctccg
     3421 ggagctcacg cgggaggggc atgtggtccg taccgaccat ccccaacacc ggatcgccgc
     3481 gaaaccaggg cgagaaccgc tcctcgaggg gggcgaggtc ggcggggccc gggacctcga
     3541 agagatacgc cgcgttcccg tacccggagg gtaggtactc cgtcacgacc gaagagccgt
     3601 ccggcccctc ccagacgaag cgatgaaaat cgacggccgc cggcactcct cgccacacca
     3661 ccgccgtctc gatcccggcg agccggagga tctgcggcat ctgggcgacg tgcccgaaca
     3721 tgtccggtag gtagccgaca cgcatcgcac tcccgaagtc cgccgcgcgc gcgagcccgg
     3781 cctcgaggtt tcgcagcgtc gtttcgccgt cgacgaggaa ctcgtccatc agggtctgcc
     3841 acggcccgat tgcgaggcgc ccagcctga
//
LOCUS       DASVEU010000058         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_429506,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000058 DASVEU010000000
VERSION     DASVEU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429506"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF225_02325"
     CDS             <1..181
                     /locus_tag="VF225_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX7144120.1"
                     /translation="AELFDTDGTAIVVHALPDNFANIPTRYQSTTEGTFGPDSATLAT
                     GDAGGRVACGVVAAG"
     gene            complement(506..844)
                     /locus_tag="VF225_02330"
     CDS             complement(506..844)
                     /locus_tag="VF225_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144121.1"
                     /translation="MSRVHVQNVRDLPVLWPYRQRRHLVVACMDDARALEALRPQIEG
                     LGAALVVERDPGPLSRRLGRPSLTVADRFGTVGLTAEEIWAERILAQLEAFELSCPEC
                     GPQAWGDPAV"
     gene            complement(1030..2907)
                     /locus_tag="VF225_02335"
     CDS             complement(1030..2907)
                     /locus_tag="VF225_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX7144122.1"
                     /translation="MKSRHRFLLLAMLAALVLAAPAASRPDRFASEEMFPGEGIAQPM
                     QHGMAEGHLPPVNQGVKLVGKAEVTNPSGAGNDGRVADVSAFGKFAFLTAFRDPTCER
                     TGAHVIDMSDPKNPFEVTSAFMETTPFNYAGEGSDTLRLKNDFFDGVLFIHQNETCPG
                     APAPTAPATRGGINIWDITDPRNPELLVKHAGDLDGGFDDQPNQTHSVFAWTSPFDGR
                     TYAVLVDDEEFTDLDILDITDPRNPFMVNDTLDLVELFGVEQESPPNLTSIFSHDMYV
                     KRVGQRLVMNVSYWDGGYVLLDVTDPTPGNVTLIAESDYPELDEERLARGHEISPEGN
                     AHQSELSPDNRWLVATDEDFNPYRVVAEITSGPHSGTEFTAISGSGVPAIDADTSISG
                     TPTFVGLACAGSPPPAGDGIALIERGVCSFQEKLDLVKAAGYDAGIVFNTVRPDCQGL
                     VFMLAEGDIPFVFVSRATGLQLLNQTLSADVCTQASPAAGSPVASVLIEAVFDGWGYV
                     RLFKTKVPGQVGAPGSIESFDTFTIPEAQDPAFAEGFGDLSVHEVALNPHPNKNLAYV
                     SYYAGGFRILKYGPGGLRQVGAFIDEGGNNFWGVEFHKMGGKEYVLASDRDFGLYIFR
                     P"
     gene            complement(3003..3659)
                     /locus_tag="VF225_02340"
     CDS             complement(3003..3659)
                     /locus_tag="VF225_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144123.1"
                     /translation="MGSEPPLYGPQQSRFARYLERREERRPARVARDLRRRLLTGLRG
                     RVLELGCGDGRAFEHYRPGVERVLAVEPDATARTAATQRARDAAAPIDVVDGDAVALP
                     AEGGAFDAAVVVWVLCSVPDPAAALRELRRVLRPGGELRFYEHVRSPHAVFRGLQRAA
                     DTLFWTRALGGCETTRDSAAAIAAAGFEIVALESGFHSSSLLTITAAPYVLGTARPSP
                     "
     gene            complement(3700..4491)
                     /gene="dapB"
                     /locus_tag="VF225_02345"
     CDS             complement(3700..4491)
                     /gene="dapB"
                     /locus_tag="VF225_02345"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7144124.1"
                     /translation="MCIAGATGWTGRALARAVLEAPDLALVSAVSRSASGGDLGEALG
                     GDALGVPVHASVREALDGVDVLVDYTSATAVKANTLAAIDAGVAVVIGSSGLTAADFG
                     EIDSAARQRSVGVVASGNFSLTAAMCQAAALLAARHLPQWEIIDYASADKPDVPSGTA
                     RELAERLGEIRRPELGHPIEDLHGPKEARGVTVGGSQVHSVRLPSFVVSTEVVFARAD
                     ERLTIRHDAGGTAEPYVEGTLLAVRKVPERVGLTRGLDALLLETE"
     gene            complement(4574..5545)
                     /locus_tag="VF225_02350"
     CDS             complement(4574..5545)
                     /locus_tag="VF225_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7144125.1"
                     /translation="MKAIVYHEYGSPDVLELREIDKPVVEDDEVLVRVRAASANPADW
                     HFMRGQPYIMRPQSGLRKPRNSVLGRDISGQVEAVGKAVTRFRPGDEVFGDVETGGFA
                     EYRCVSEGFLGPKPANLTFEQAAAVPLAALTALQGLRDAGQVQPEQKVLIIGASGGVG
                     TFAVQIAKWLGADVTGVCSTRNVEMVRSLGADHVIDYTQEDFTQSGQKYDLIFQLAGT
                     RSPSDCRRALTPKGTLVLSSGESDGRWIGPVDRIIKAVVLSPFVSQKLGSFLAKPNEE
                     DLQFLKELIEAGKVKPVIDRRYPLSEAPEAIRYLEEGHARGKVVITV"
     gene            5677..6111
                     /locus_tag="VF225_02355"
     CDS             5677..6111
                     /locus_tag="VF225_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144126.1"
                     /translation="MKDTPVSDTLADIPTAAVPHTDARWQPIRNHFGLTAFGINGYTA
                     LRAGDRIIGEHDHSNPDDEQHQELYFVHSGRARFEVDGAVIDAPAGTFVSAQDVGTRR
                     SAIAEEDNTVVLVIGAEPGAAFEITPAEREELTDAGLSVRPA"
BASE COUNT         1005 a         2168 c         2051 g          986 t
ORIGIN      
        1 tgcggagctc ttcgacacgg acggaacggc gatcgtcgtg cacgcgctgc ccgacaactt
       61 cgccaacatc ccgacgcgct accagtcgac gaccgagggc accttcgggc ccgattcggc
      121 gacgctcgcg acgggcgacg caggcggccg cgtggcctgc ggagttgtgg cagccggctg
      181 acggttcctc cgggcacagg gcgaggcggg aggacctcgg cgcctcccgc ccagcgcccc
      241 aggagggttc gtcgaagggt tcgtccggac gccgccggac gccgccgtag catctccgag
      301 cgagtcgggc gcaagccctc cggacaggat tccggcgcac gatcctcgac tcgggttggc
      361 tgacgaaggc gcgcgtgggc acggatcgac catgtacgcc ggcagtcgtg gcaaagaggc
      421 gtgaggtgac gccgtaacac gtggttcgcc gggctctatc gtcgcagccc gccagcgacc
      481 gaacggctac ctcgccaagc tccgcctaga cggccggatc gccccaggcc tgaggcccgc
      541 actcggggca ggagagctcg aacgcctcga gctgcgccag gatgcgctcg gcccatatct
      601 cctccgcggt cagcccgacc gtgccgaagc ggtccgcgac cgtgagggac gggcgcccga
      661 ggcgcctcga gagcggcccg gggtcacgct cgacgacgag cgctgcgccc agcccttcga
      721 tctgcggccg cagagcctcg agcgcacgag cgtcgtccat gcacgccacg acgaggtgac
      781 gccgctgtcg atacggccag agaaccggga gatcgcggac gttctgcacg tggactcgag
      841 acactcgacc aaccgtagcg tcgtcgcgcg ggcatcggcg cggtcttctt cgcgcgttct
      901 gggccaccgg caaccgcgag cagggtggat cgcgttcatc gcgggcggcg caaccgtttc
      961 ggcgttcgcc ccctgcatgg gacggcgggg gcgctggccc ccgccgtccg tcgctcgtcg
     1021 tcagacccgc tagggcctga agatgtacag gccgaagtcc cggtcgctcg ccaggacgta
     1081 ctcctttcca cccatcttgt ggaactcgac gccccagaag ttgttgccgc cttcgtcgat
     1141 gaacgcaccg acctggcgga gccctcctgg accgtacttg agaatccgga acccgcccgc
     1201 gtagtagctg acgtacgcga ggttcttgtt cggatgcggg ttgagcgcga cctcgtgcac
     1261 cgacagatcg ccgaagccct cggcgaaggc cggatcctgc gcctcgggga tcgtgaaggt
     1321 gtcgaacgac tcgatcgagc ccggtgcgcc gacctggccg ggcaccttgg tcttgaagag
     1381 ccgcacgtag ccccagccgt cgaagaccgc ctcgatcagc acggaggcca ccggcgagcc
     1441 cgccgccggc gaggcctgcg tgcagacgtc ggcggagagc gtctggttga ggagctgcag
     1501 cccggtcgcg cgactcacga acacgaacgg gatgtcgccc tcggccagca tgaagacgag
     1561 cccctggcag tcgggccgca ccgtgttgaa gacgatgccg gcgtcgtagc cggcggcctt
     1621 cactaggtcg agcttctcct ggaacgagca cacgccgcgc tcgatgagcg cgatgccgtc
     1681 acccgcgggc ggcgggctgc cggcacacgc gagcccgacg aacgtcggcg tcccgctgat
     1741 gctcgtgtcc gcgtcgatgg ccggcacacc cgaccccgag atcgccgtga actcggtccc
     1801 gctgtgcggg ccggacgtga tctcggcgac gacgcggtag gggttgaagt cctcgtccgt
     1861 ggcgacgagc cagcgattgt ccggcgagag ctcggactgg tgggcgttgc cctccggcga
     1921 gatctcatgg ccgcgggcca gcctctcctc gtcgagctct gggtagtcgg actcggcgat
     1981 gagtgtcacg ttccccgggg tcgggtcggt cacgtcgagg agcacgtagc cgccgtccca
     2041 gtagctcacg ttcatgacca ggcgctggcc gacgcgcttc acgtacatgt cgtgcgagaa
     2101 gatcgacgtc aggttcggcg gcgactcctg ctcgaccccg aacagctcga ccaggtcgag
     2161 cgtgtcgttc accatgaacg gattcctcgg atcggtgatg tccaggatgt cgagatcggt
     2221 gaactcctcg tcgtcgacga ggacagcgta ggtccggccg tcgaacgggc tcgtccacgc
     2281 gaagacgctg tgggtctggt tcggctggtc gtcgaacccg ccgtcgaggt caccggcgtg
     2341 cttcacgagc agctcggggt tcctcgggtc ggtgatgtcc cagatgttga tcccgccgcg
     2401 ggtcgccgga gccgtcggcg ccggagcgcc cgggcaggtc tcgttttggt ggatgaagag
     2461 gacgccgtcg aagaaatcgt tcttcagccg taaggtgtcc gagccctcac ccgcgtagtt
     2521 gaagggcgtc gtctccatga acgcgctcgt cacctcgaac gggttcttcg gatccgacat
     2581 gtcgatcacg tgggccccgg tgcgctcgca cgtcgggtca cggaaggcgg tgaggaacgc
     2641 gaatttgccg aaggcggaca cgtcggcgac gcggccgtcg ttcccggcgc cggacgggtt
     2701 cgtgacctcc gccttgccga ccagcttgac tccttgattc acgggcggca ggtggccctc
     2761 cgccattccg tgctgcatcg gctgggcgat gccctcgccg ggaaacatct cctcgcttgc
     2821 gaagcggtcc ggacgactgg ccgccggcgc agccagtacc aaagccgcga gcatggcaag
     2881 gagaagaaat cggtgcctgc tcttcatagc tcctcccagg gtggtggtga aggcgtttta
     2941 taccgtgttg caggagaaaa gtgaatagcc tgtagcgggt gggcacaccg cctcggcgcc
     3001 gttcagggcg acgggcgcgc ggtgccgagg acatacggcg ccgcggtgat cgtgaggagc
     3061 gacgacgagt ggaatccgct ctcgagcgcg acgatctcga accccgcggc ggcgatcgcg
     3121 gcggcgctgt cccgcgtcgt ctcgcagccg ccgagcgccc gcgtccagaa gagcgtgtcg
     3181 gcggcgcgct ggaggccccg gaagacggca tggggggagc gcacgtgctc gtagaagcgg
     3241 agctcgccgc ccggcctcag cacccgccgt agctcgcgga gcgcggcggc tggatcggga
     3301 acgctgcaga gaacccaaac cacgactgcc gcgtcgaatg ctccgccctc cgccggcagc
     3361 gccacggcgt cgccgtccac gacgtcgatc ggagccgcgg cgtcgcgcgc ccgctgcgtc
     3421 gcagccgtcc gcgcagttgc gtccggctcg acggcgagca cgcgctccac gcccggccgg
     3481 taatgctcga atgcgcggcc gtcgccgcag ccgagctcga gcacccgccc gcggagcccc
     3541 gtgagcaatc gccgccggag atcgcgcgcc acccgagcgg gccgccgctc ctctcgtcgc
     3601 tcgaggtacc gcgcgaagcg cgactgctgc ggcccgtaga gcggtggctc actccccacg
     3661 gggcgatgtc ccccgttgca agccgagccg gcgcggccgt cattccgttt ccagcagaag
     3721 agcgtcgagc cctctcgtga gcccgacccg ctctggcacc ttgcgtaccg cgagcaacgt
     3781 tccctcgaca tacggttcag ccgttccccc cgcatcgtgc cggatcgtca ggcgctcgtc
     3841 ggcacgggcg aaaaccacct ccgtcgacac gacgaagctc gggaggcgta cggagtgcac
     3901 ctgggagccg ccgacggtga ctccacgggc ctccttgggc ccgtggagat cctcgatcgg
     3961 atgcccgagc tccgggcggc gaatctcccc gagccgctcc gcgagctccc gtgccgtccc
     4021 gctcggaacg tcgggtttgt cggcgctcgc atagtcgatg atctcccact gcgggaggtg
     4081 gcgcgccgca aggagagcag cggcctggca catcgcagcg gtcagagaga agttccccga
     4141 cgccaccaca ccgactgagc gctgccgggc cgccgagtcg atctccccga agtctgcagc
     4201 cgtcagaccc gagctcccga tcaccacggc gacaccggcg tcgatcgccg ccagcgtgtt
     4261 cgccttgacc gccgtcgccg acgtgtagtc gacgaggacg tcgacgccgt cgagcgcctc
     4321 gcgcacgctc gcgtggactg gaactcccag cgcatcgccg ccgagcgcct cgccgagatc
     4381 tccgccggac gcggatcggc tgacggcgga gacgagcgcg aggtcgggcg cctccagcac
     4441 ggcccgcgca agcgcccgtc ccgtccagcc ggtggctccc gcgatacaca ccgacaagcg
     4501 ctcagtctgc ggaatggctc agcctcgctt tcgatggatc agggcgccta aacccgctag
     4561 ccggcccgac gcctcacacg gtgatgacaa ctttcccccg cgcgtgtccc tcttccagat
     4621 atcggatggc ctccggcgct tcgctcagcg ggtatctcct gtcgatgacc ggcttgacct
     4681 tcccagcctc gatgagctcc ttcaggaact gcaggtcctc ctcattgggc ttcgccagaa
     4741 acgagccgag cttctggctc acgaacggtg acaacacgac cgctttgatg atgcgatcca
     4801 ccggtccgat ccaacgaccg tccgattctc cgctgctcag cacgagcgtc cccttggggg
     4861 tgagcgcgcg cctgcagtct gacggggaac gcgtccccgc caactggaag atgagatcgt
     4921 acttctgccc actctgggtg aagtcctctt gggtgtagtc gatgacgtgg tccgcgccga
     4981 gcgagcgaac catctccacg ttcctcgtgc tgcacacgcc ggtcacgtca gcgcccaacc
     5041 acttcgcgat ctgcacggcg aacgtcccca cgccccccga cgcaccgatg atcaagacct
     5101 tctgctctgg ctgaacctgt cccgcgtcac ggagaccttg gagagctgtc aacgcagcca
     5161 gaggcaccgc cgccgcttgc tcgaacgtca ggttggccgg tttcggcccc aagaagccct
     5221 ccgagacaca cctgtactcg gcgaagccac ccgtctcgac atcgccgaac acctcatcac
     5281 ctgggcgaaa ccgagtcacg gccttgccga ccgcctcgac ctgccctgag atgtcgcgtc
     5341 cgagaacgct gttccttggt tttctcagcc cggactgggg gcgcatgatg tacggctggc
     5401 ctctcatgaa gtgccagtca gcgggattgg cggaggctgc gcggacgcgt acgagcacct
     5461 cgtcgtcctc gacgaccggc ttgtcgattt ctcggagttc caggacgtca ggtgagccgt
     5521 actcgtggta gacgatcgcc ttcattcgcg ttgccctgcg cccccgcggg tcagtctgcc
     5581 tcgatcagct cgatccggta gccgtccagg tatcgacctt ttgcaaactg acgactgtgg
     5641 ttgaatgtag cgcctccgac cactgccgag gctcacgtga aagatactcc agtttccgac
     5701 acgctcgccg acatcccgac agcagccgtt ccacacaccg acgcccgctg gcagccgatt
     5761 cgcaaccact tcgggctcac ggcattcggc atcaacggat acacggcgct gcgtgctggc
     5821 gaccgcatca tcggggagca cgaccactcg aaccctgatg atgagcaaca ccaggagctg
     5881 tatttcgtcc actccggccg agcgaggttc gaggtcgacg gcgcagtcat tgatgcccca
     5941 gcgggtacgt tcgtgagcgc acaagacgtt ggcacaaggc gatccgcgat cgccgaagaa
     6001 gacaacacgg tcgtgctcgt catcggagcg gagcctggcg cggctttcga aatcaccccg
     6061 gcagaacgcg aggaactcac tgacgctggc ctgtcggtgc gtccggcgta acgcctgcta
     6121 cgcgcgctca ataatttcga cgcgataacc gtcggggtcg cggacgaacg tcagtcgcgc
     6181 gccgccctcg cggacggtgt acggggggcg
//
LOCUS       DASVEU010000059         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_431503,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000059 DASVEU010000000
VERSION     DASVEU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431503"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..821
                     /gene="ubiE"
                     /locus_tag="VF225_02360"
     CDS             138..821
                     /gene="ubiE"
                     /locus_tag="VF225_02360"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7144127.1"
                     /translation="MTHESGTLPAEGVRSMFDRIAPVYDLMNRVMTVGLDQRWRRLTV
                     ERVVARGDRVLDACCGTGDLAVAARKAGADVVGLDFSGRMLERAREKDPAIEWIQGDL
                     LELPFEDASFDAATVGFGVRNVADLEQALTELRRVLRPGGRVGILEITRPRGPLAPFY
                     RLWFDGVVPLLGKLIPGGSAYTYLPASVRRFPGPAELAEMIAAAGFRDVRARLFAGGI
                     VALHTAAVE"
     gene            827..1723
                     /locus_tag="VF225_02365"
     CDS             827..1723
                     /locus_tag="VF225_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7144128.1"
                     /translation="MAEIRDAPGLDAYLGDLEERLEQAVGAYRGVVAEVGAEALAAGG
                     KRLRPLLVYLSTPPGSPPPVVAGIAVELVHLATLMHDDLIDGARLRRGHASAWSAHGP
                     DAARAAGDYLFARAFAELAATGDTQAVAVLANATLCLARGEALQRSQRFDPDTRVEAY
                     LERCTLKTGKLFEAACRLAGGSGAFGLALGVAFQITDDILDCSGVTIETGKIAGTDLR
                     EGTPTLPLLLAAQRDEAVREALAGRSQLEGALLRVASTGAIEESREVALAYARNARAC
                     LNGELHREELESLTYAVVDRER"
     gene            1723..>2631
                     /locus_tag="VF225_02370"
     CDS             1723..>2631
                     /locus_tag="VF225_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX7144129.1"
                     /translation="MALLERTDLLAPIRDKVETGERLDFDDGVTLMESDDILALGELA
                     DLARRVRGGDDRVYFVQNLYLNQTNVCRVKCKFCAFAVTNKQSDAYTYTPDELVEDAI
                     HQHELTGFTEIHMVGGESPHVDFDYYVGIVRSLHEALPDVHLKLFTASEIHHMTKLSG
                     LTHEEVLRELQAAGLGSLPGGGAEVFADRVRRLVAPGKEHPDFWFHTHDTAHRLGIPT
                     HCTMLYGHVETYEERVDHLLRLREQQDKTGGFLAFIPLAFHPENTVFERRGWKHTTGS
                     DDLKMIAVSRLLLDNIANVKAYWIMMG"
BASE COUNT          406 a          891 c          903 g          431 t
ORIGIN      
        1 ggagcgacga tcctgttcgc ggcgccgggc ttctaccacg gcgcggagac catcgacgac
       61 ctcgtcgact tcatcgtcgc gcggatcctc gatcagctgg ggctcgacaa cacactcatt
      121 tcgcgctggg gacagtcgtg acgcacgagt ccggcaccct ccccgcggaa ggcgtccgct
      181 cgatgttcga ccggatcgct ccggtctacg acctgatgaa ccgcgtgatg accgtcgggc
      241 tcgaccagcg ctggcggcgg ctcacggtcg agcgcgtcgt tgcgcgcggc gaccgcgtcc
      301 tcgacgcctg ctgcggcaca ggcgacctcg cggtcgcggc gcggaaggcc ggtgcggacg
      361 tggtcggact cgacttctcg ggacggatgc tcgagcgcgc ccgggagaag gatcccgcga
      421 tcgagtggat ccagggagat ctgctggagc tgccgttcga agacgcgtcc ttcgatgccg
      481 cgacggtcgg gtttggcgtc cgcaacgtgg ccgacctcga gcaagcgctg accgagctgc
      541 ggcgcgtgct ccgtcccggc ggccgggtcg ggatcctcga gatcacgcgt ccgcgcggtc
      601 ctctagcgcc gttctaccgt ttgtggttcg acggagtcgt acctttgcta ggcaagctga
      661 ttccaggtgg atccgcgtac acgtacctgc ccgcgagcgt ccgtcgcttt cccggtccgg
      721 ctgagctcgc ggagatgatc gcggcggcgg ggttccgcga cgttcgcgcg cgcctcttcg
      781 cgggcgggat cgtcgcactg cacacggcgg ctgtcgaatg agtgcattgg ccgagattcg
      841 cgacgcgccc ggcctcgacg cctatctcgg cgatctggag gagcggctcg agcaggccgt
      901 cggcgcctat cgaggtgtcg tcgcggaagt tggggcggag gcactcgcag cgggcggcaa
      961 gcggctgcgg ccgctgctcg tgtacctctc cacaccgcct ggatccccac cacccgtcgt
     1021 cgcaggcatc gcggtcgagc tcgtccatct ggcgaccttg atgcacgacg acctgatcga
     1081 cggcgcgcgc ctacggcgcg ggcacgcgtc ggcctggtcg gcgcacggcc ccgacgccgc
     1141 ccgcgcggcc ggggactatc tcttcgcccg tgcgttcgcc gagttggccg cgaccggcga
     1201 cacgcaggcg gtggccgtgc tggcgaacgc cacgctctgc cttgcgcgtg gcgaggcgct
     1261 gcagcgttcc cagcgcttcg atccggacac cagggtcgag gcgtacctcg aacgttgcac
     1321 gctgaagacg ggaaagctgt tcgaggctgc gtgccggctg gccggcggct ctggtgcttt
     1381 cgggctcgcc ctcggcgtcg cgttccagat caccgatgac atcctcgact gctcgggcgt
     1441 cacgatcgag accgggaaga tcgccggcac cgatctgcgc gaggggacgc cgacgctgcc
     1501 gttgctgctc gctgcgcaac gggacgaggc cgtgcgcgag gcgcttgcag gtcgctccca
     1561 gctggaaggt gcgctgttgc gcgtcgcttc gacgggggca atcgaggaga gccgcgaggt
     1621 cgcgcttgca tatgcgcgca atgcccgcgc gtgtctcaac ggcgagctgc accgcgaaga
     1681 gctcgagtcc ctgacctacg ccgtcgtgga ccgcgaacgc tgatggcgct actcgaacgc
     1741 accgacctgc tcgctcccat ccgcgacaag gtggaaaccg gagagcgcct cgacttcgac
     1801 gacggggtga cgttgatgga gagcgacgac attctggcgt tgggcgagct cgccgatctt
     1861 gcgcgacgcg tgcgcggagg cgacgaccgc gtctacttcg tccagaacct ctacttgaac
     1921 cagaccaacg tctgtcgggt gaagtgcaag ttctgcgcct tcgcggtgac gaacaagcaa
     1981 tccgacgcct atacgtacac gccggacgag ctcgtcgagg acgcgatcca ccagcacgag
     2041 ctcacagggt tcaccgagat ccacatggtc ggcggcgaga gcccgcacgt cgacttcgac
     2101 tactacgtcg ggatcgtccg ctcactgcac gaggccctgc cggacgtgca cctgaagctc
     2161 ttcacagcca gcgagatcca ccacatgacg aagctctccg ggctcaccca cgaggaggtc
     2221 ttgcgcgagt tgcaggcggc cgggctcggc tcgctgcccg gcggcggcgc ggaagtgttc
     2281 gccgaccgcg tgcgacgctt ggtggcgccg ggtaaggagc atccggactt ctggttccac
     2341 acgcacgaca cggcgcaccg gctcgggatt ccgacgcact gcacgatgct ctacgggcac
     2401 gtggagacct acgaggagcg cgtcgaccac ctcctgcggc tgcgcgagca gcaggacaag
     2461 accggcggct tcctcgcgtt catcccgctg gcgttccatc cggagaacac cgtcttcgag
     2521 cggcgcggct ggaagcacac gaccggttcg gacgacctga agatgatcgc agtgtcgcgc
     2581 ctcctgctcg acaacatcgc gaacgtcaag gcctactgga tcatgatggg c
//
LOCUS       DASVEU010000060        13582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_819891,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000060 DASVEU010000000
VERSION     DASVEU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819891"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VF225_02375"
     CDS             complement(<1..719)
                     /locus_tag="VF225_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX7144130.1"
                     /translation="MIWSWLHRHPRLVDIWIVGVLLALGLGATIRNGHGATGVVLSVA
                     ETAPLLFRRRYPVAVVVVVTAAVLVGIAAGVWVLPLQLGVALYTLTSLREQSAERAVG
                     IAAIFAVAITVLSVGGIEFGAGAARVVFLIAAALLGDSIRSRRAYVREIEEKADRLER
                     ERESEARRAAAEEQARIARELHDIIAHALSVIIVQAGAADDAFELDPARAREPIRAID
                     SAARAALADLRRVLGILQESA"
     gene            complement(765..1595)
                     /locus_tag="VF225_02380"
     CDS             complement(765..1595)
                     /locus_tag="VF225_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144131.1"
                     /translation="MSSAIAPLALPRLEERSRVTQARVALSEWTKFVSLRSTRWSLGV
                     GILLTIALPVVFAAVTGSHWAHMSAAERADRHPLDIALAGVNVAQLAIAVLGVLVISG
                     EYSTGMIRSSLIAVPKRLPVLWAKIAVYAAVSFILTLPAVIIAFFASQAILSRHHILQ
                     ISFGDPGVARSVLGGALYVTLVGIFALGIGTILRNTAGGIAAFAGIFFVIPPLMNILP
                     TSWNNAISQYLPSEAGRQLFSLHHAAHSLSPTAGGLLMLGYCALTVTVAAVVLVRRDA
                     "
     gene            complement(1592..2530)
                     /locus_tag="VF225_02385"
     CDS             complement(1592..2530)
                     /locus_tag="VF225_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7144132.1"
                     /translation="MIEATNLTKHYGEKAAVADISFEVRPGIVTGFLGPNGSGKSTTM
                     RLILGLDAPTSGDVRVNGKHYRDHAAPLHEVGALLEARSVHTGRSAYNHLLALAQTHG
                     IGRRRVQEMIDLVGLHDVARKRAGQFSLGMGQRLGIATALLGDPATVMLDEPVNGLDP
                     EGVHWIRNLLKQLAAEGRTVFVSSHLMSEMAVTADHLIVIGRGRLIADMSVEEFVQKA
                     SGTRVRVRTPQATRLRELVLGPHVSVTSTESGVIEVEGLTAEQVGEIAAEHTIVLHEL
                     TPVQASLEEAFMELTREEVEFKSVGGDLVQSEELAA"
     gene            complement(2613..3431)
                     /gene="dprA"
                     /locus_tag="VF225_02390"
     CDS             complement(2613..3431)
                     /gene="dprA"
                     /locus_tag="VF225_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7144133.1"
                     /translation="MRVLRRRELPPLLRAIHDPPARLYLRGNGDVTLLAEPAVAIVGA
                     RACSPYGAQVARMLGRELAGADVVVVSGLARGIDGEAHRGALETEGHTVAVLGCGIDR
                     DYPAAHTMLARSIGERSLIVSEYEAGVEPAPWRFPARNRIIAGLCAVTVVVEARERSG
                     ALITADFALEEGREVFVVPGEITSTLSAGTNALLRLGATPLTAAADVLESFGIQSPPN
                     RTVEGNPLLDLLPATADELVQKTGLGVGEVMAALAELDVAGLASEGDGIYRRLA"
     gene            complement(3428..4885)
                     /locus_tag="VF225_02395"
     CDS             complement(3428..4885)
                     /locus_tag="VF225_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7144134.1"
                     /translation="MLARAVTFALVGLEPRRVEVEAHLQGGTPSLTIVGLADRACQEA
                     RQRVRSGILSAELEFPGGHVTVNLAPAELKKEGSGFDLPIALALLAASHQLPRDRLAE
                     HASCGELALDGRLRRVGGVLAVAEGARRAELERIICPYEACAEAALAGVEPIGMRHLA
                     DAVAYLRGELEPPEYVVGNGKPRRRPLDLADVRGQERGRRALELAAAGRHNLLLGGPP
                     GTGKTMLARRLPGILPPLEEEQALEVTRIHSVAGLVDPDHPLIGYAPFRAPHHSASTA
                     AIVGGGPRLRPGEASLAHRGVLLLDELAEFQRPALEALRQPLEDGELVVARAAGSVRF
                     PARFQLVGTMNLCPCGARGDPGAQCSCSAQRLARYRDKLSRALLDRFDLVVTIPRARA
                     LELQGGPGERSDPVRSRVVAARLRLEETAPKRTSAADELLTRAVERLPLSGRGRAKVA
                     GVSRTIAALAGSDAVLPEHVAEALAYRAPSELATP"
     gene            complement(4928..6034)
                     /locus_tag="VF225_02400"
     CDS             complement(4928..6034)
                     /locus_tag="VF225_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX7144135.1"
                     /translation="MAPTQDALYAELDLALSWSERDLPERERTKHVHRLHPYLGKFIP
                     QLVEILLGRYLRPGHHVLDPFAGSGTTLVQALESGLDATGVDVAAFNCLLIDVKTRPY
                     NPFVLEQELRAVHARVGETAVERPAAYIRRWYAPQAARELLGFRALIDDYEHADVLRV
                     VLARAARSARRTTHFDLEFPREPQLEPYWCHKHRRECRPVETASQFLRRYTLDTLARL
                     KEFSRVRARGRCASIIHGDVREEQLPGRYDGVLTSPPYPGLIDYHEQHRYAYELLGLD
                     DRRELEVGAAAAGNSRAALAAYSDGIVEVLLNTKSVLRRGAPIVIVVNDRRELYPEIL
                     ERAGLRLEERLRRHVNRRTGRRAGEYFEDVLIAR"
     gene            complement(6057..6395)
                     /locus_tag="VF225_02405"
     CDS             complement(6057..6395)
                     /locus_tag="VF225_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7144136.1"
                     /translation="MSVRPGKRAEQRAVWWYRLRGYRILATNAWVAGYELDIVVRRGR
                     RVIFCEVKEKLGERFGDPLEMVDDEKQRRIRLAADAWLARHPETTGLQVSFDVIAVRS
                     GQLERVAQAF"
     gene            complement(6431..7270)
                     /locus_tag="VF225_02410"
     CDS             complement(6431..7270)
                     /locus_tag="VF225_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144137.1"
                     /translation="MPRRLLATAAICIGCAATAANADGGGPGAGVQQGWDGLANGAVR
                     YVAVPAAGSTALEVIRRQDGRVLKFMALKGTWGIPVVAFDGTADGLMGDGRTLLLGEP
                     TTGPALRKHSTFALVDLKSMKLVRKLRLPGHQVFDALSPDGRYLYFIEYVSAEDFNRY
                     RVRAYDLHAGRLLARAVIDKRESETAMQGAPISRVTSQDRTWAYTLYGGPDKTFIHAL
                     DTMHAGAVCIDLPWKSQPKRLFEFRIRRAGDGRLVVRGPRGRTLAVVDRNNLHVVNAV
                     RDP"
     gene            complement(7330..7791)
                     /locus_tag="VF225_02415"
     CDS             complement(7330..7791)
                     /locus_tag="VF225_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7144138.1"
                     /translation="MSEHSAFGDDPFGRAAERAARFFGTPQYIIGQTLIVIAWIALNS
                     VALMAHWDPYPFILLNLAFSTQAAYAAPLILLAQTRQASRDKVMGEKLETHREKTEHN
                     AEQRVARLKRETDKLEALLEANTDLTKQDKELTEQVAGLTREIHALLTKQA"
     gene            complement(7810..8469)
                     /locus_tag="VF225_02420"
     CDS             complement(7810..8469)
                     /locus_tag="VF225_02420"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7144139.1"
                     /translation="MAPRTGQKLLKFDRQLGARLVAGTDEAGRGCLAGPLVVAGVLLD
                     YDCLRDHKVRPLGYLNDSKQCSPERREELFHAVLGCAEQVVVRVIPHGAIDRNGLHKS
                     NLAAMREILHTLSPPAEACLVDGFRLGPTAPPHRAIVDGDEKSAAIAAASIVAKVTRD
                     RLMRRMDALFPHYGFSSHVGYITPGHSRIVRERGPSRIHRRSFQAMCYLKEEELAALS
                     A"
     gene            complement(8575..8919)
                     /gene="rplS"
                     /locus_tag="VF225_02425"
                     /pseudo
     CDS             complement(8575..8919)
                     /gene="rplS"
                     /locus_tag="VF225_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(8992..9876)
                     /gene="lepB"
                     /locus_tag="VF225_02430"
     CDS             complement(8992..9876)
                     /gene="lepB"
                     /locus_tag="VF225_02430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7144140.1"
                     /translation="MATGTEPAADRHVSDHYALLGVRSDASPQEIESAYRRGALELGY
                     QDGAWHELRDAYDVLRDPEERARYDAERDGFGPPPAAPQKKSHNPIDRFFPNLPHGWR
                     VGIDWVVTIAGAIAIVLAIKAWIVNPYRIPSSSMEPTLHCARPAQGCEAATSDRVLAN
                     RFIYRFRNPKRGEIVVFTTPSLAKQECGSEGTFVKRLIGLPGDVWEERAGYVYIDGKK
                     LNEPYVKADRRDTQTLSLRDIPPRNTYTRIPKDYYLMMGDNRSSSCDSRRWGLVPRKN
                     LIGKVFATYWPPGRISFH"
     gene            complement(9837..10493)
                     /gene="trmD"
                     /locus_tag="VF225_02435"
     CDS             complement(9837..10493)
                     /gene="trmD"
                     /locus_tag="VF225_02435"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7144141.1"
                     /translation="MQLDVFTLVPHAFAWLTEQRPVASVLGTELDLRLFSYRDTTPLR
                     AAQVDDEPYGGGAGMVLRVDVVAAALETVYGGVPDRRVIALTPQGRQLTQDVVEELAD
                     EERLTLLSARFEGFDERVVQHLASDAISIGPYVLSNGDLPAMVLVDAIARRLPGAIKE
                     ESGAFESFSSELDGGLEYPHYTRPSEFRGWHVPEVLLSGDHGKIEEWRREQSRRRTVT
                     "
     gene            complement(10510..10983)
                     /gene="rimM"
                     /locus_tag="VF225_02440"
     CDS             complement(10510..10983)
                     /gene="rimM"
                     /locus_tag="VF225_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7144142.1"
                     /translation="MSASQTVVVGRVGKSHGLDGSFVVENASDAPERFEVGAELIVEG
                     EPAKVVARRHARGRPVIRLDRRVPRGAELEVPREALAPAGEDEYYVFQLVGLRVQEDG
                     GDELGTVTNVVPGVANDVLELDSGIALPMVEDCVRVVDLAAGRITVAPGFSGAAS"
     gene            complement(10999..11229)
                     /locus_tag="VF225_02445"
     CDS             complement(10999..11229)
                     /locus_tag="VF225_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7144143.1"
                     /translation="MAELLEYLARQLVDEPDAVRVERVEEADAVVLRLHVAQDDLGKV
                     IGRQGRIARALRTIVRAGSVREHRRVQLEIVD"
     gene            complement(11232..11465)
                     /gene="rpsP"
                     /locus_tag="VF225_02450"
     CDS             complement(11232..11465)
                     /gene="rpsP"
                     /locus_tag="VF225_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7144144.1"
                     /translation="MRLTRVGSKKNPVYRVVVADSRSPRDGKFIEIVGRYNPQHDPSL
                     IEFDEDRVKDWLGKGALPSEAVSKLLKIRNLNR"
     gene            complement(11480..12802)
                     /gene="ffh"
                     /locus_tag="VF225_02455"
     CDS             complement(11480..12802)
                     /gene="ffh"
                     /locus_tag="VF225_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7144145.1"
                     /translation="MFDTLSDRLQGALGDLRGRGKLDDEAISRAMREIRLALLEADVN
                     FEVVKVVHDELTAVMGSGSSQLAFTGRPPTVILLAGLQGSGKTTAAAKLALMLRKEGR
                     SPGLVAADLQRPAAIDQLEQLGTQIQVPVYRGEGTDPVAVVRDGLRQLSDDGVDTIIL
                     DTAGRLHVDEELMQELERVRDEAKPANVLLVVDAMTGQEAVNVALAFQERIAFDGVVM
                     TKLDGDARGGAALSVKAVTGRPIKLISVGEKLDQLEYFHPDRMASRILGMGDVLTLIE
                     RAEEAVEEDEQKDLEARMMKGQFTFDDFLKSYKMLRRMGPIQGVLKMIPGLGKQLEGM
                     DEVDEKQMGRVEAIILSMTPQERGLPKVIDGSRRLRIANGSGTTVEEVNRLLDARKQM
                     EKLMKGMSKGKLPDLGALTGQAPAATPQPGQRKATKSNKRKKRQKSRR"
     gene            12848..>13582
                     /locus_tag="VF225_02460"
     CDS             12848..>13582
                     /locus_tag="VF225_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7144146.1"
                     /translation="MPDHSERKLATVMFADLVDSTAAAGDQDPERTRARLERFYDAMS
                     AEIEGAGGTVEKFAGDAVMAAFGAPEALEDHAERALHTALAMQRRLEAVFAGDLSLRI
                     GVNTGEVVAGRPREGSSFVSGDAVNVAARLEQAAEPGEILAGERTVSAVRGAFEFGEP
                     KTVEAKGKTGGVACRPLVRGLSLMRTRGVGGLARAFVGRDEQLELLQSAYRRAVEGSH
                     PVLVTIVGDPGVGKTRLVRELWEQLAD"
BASE COUNT         2211 a         4693 c         4482 g         2196 t
ORIGIN      
        1 tcggcggatt cctggagaat cccgagcacg cggcgaaggt cggcgagtgc ggcgcgcgcg
       61 gcggagtcga tcgcgcggat cggttcccgt gcgcgcgcgg gatcgagctc gaacgcgtcg
      121 tcggccgcgc ctgcctggac gatgatcaca ctgagcgcat gggcgatgat gtcgtgcagc
      181 tcgcgggcga tccgtgcctg ctcttcggcg gctgcgcgcc gcgcctcgct ttctcgctct
      241 cgctcgagcc ggtccgcctt ctcttcgatc tcgcggacgt aggcgcggcg cgaacggatg
      301 ctgtccccga gcagcgccgc tgcgatcaga aagacgacgc gagcggcacc ggcgccgaac
      361 tcgattccac cgacggagag gacggtgatc gcaactgcaa agatcgcggc gattccgact
      421 gcgcgctcgg cactttgctc acgaagggac gtgagcgtgt agagcgcgac cccgagctgt
      481 agcggcagaa cccagacacc cgcggcgatc cccacgagca ccgcggccgt cacgacgacc
      541 accacggcaa ccggatagcg gcggcgaaag agcagagggg cggtttcggc gacgctgagc
      601 acgactccgg ttgctccgtg tccgtttctg atcgtcgcgc caagcccgag ggcgagcaga
      661 accccgacga tccagatatc gacgagccgc gggtggcggt gcagccagct ccagatcatc
      721 gccgctccac cgtaacgccc aggggtccgg tgtgcggtgc gccgctaagc gtcgcggcga
      781 acgagcacca ccgcggccac ggtgaccgtc agagcgcagt agccgagcat cagcaggccg
      841 cccgccgtgg gcgaaagcga atgcgcagcg tgatggaggg agaacagctg cctccccgcc
      901 tcgctcggca agtactggct gatggcgttg ttccagctcg tcggcagaat gttcatcagc
      961 ggtggaatca cgaagaagat ccccgcgaat gccgcgatcc caccggccgt gttccgcagg
     1021 atcgtgccga taccgagcgc gaaaattccc accagagtca cgtagagcgc accgccgagc
     1081 acggaccgtg caacgcccgg atctccgaat gagatctgca gaatgtgatg acgcgagaga
     1141 atggcctggc tcgcgaagaa cgcgatgatc accgccggca gcgtcaagat gaacgacacg
     1201 gcagcgtaga ccgcgatctt cgcccacagc acgggaagcc gcttcggcac tgcgatcagc
     1261 gacgagcgga tcatccccgt cgaatattca ccgctgatca cgagcacgcc gagtacggca
     1321 atcgcgagct gcgcgacgtt caccccggca agagcgatgt cgagcgggtg tcgatcggct
     1381 cgttccgccg ccgacatgtg cgcccaatgc gacccggtca cggcggcgaa cacgaccggc
     1441 agagcgatcg tgagcaggat cccgactccg agcgaccagc gcgtcgagcg aagcgaaacg
     1501 aacttcgtcc actccgacag tgcaacccgg gcctgcgtga cccggctccg ttcctcgaga
     1561 cggggaagcg ctaggggtgc gatcgcactg ctcatgctgc taactcctcg ctttggacga
     1621 gatcgccgcc gacggatttg aactccactt cttcgcgcgt caattccatg aatgcttcct
     1681 ccaacgacgc ctggacgggg gtcagctcgt gcaggacgat cgtgtgctca gcggcgatct
     1741 cgcccacctg ctcggccgtc agcccctcca cctcgatcac gcccgactcg gtgctcgtca
     1801 ccgagacgtg aggcccgagc accagctcgc gcaggcgtgt cgcctgagga gtccgcacgc
     1861 gcacccgtgt cccagatgcc ttctgcacga actcctcgac gctcatgtcg gcgatcagcc
     1921 gaccccgccc gatcacgatc aaatgatcag cagtgacggc catctcgctc atcagatgcg
     1981 acgagacgaa cacggtccgc ccctccgcag cgagctgctt gagcaggttg cgtatccaat
     2041 ggacgccctc cgggtcgagc ccgttcacag gctcgtcgag catcaccgtc gccggatccc
     2101 cgagcaacgc cgtcgcaatc ccgagccgct ggcccatgcc gagcgagaac tgccccgcgc
     2161 gcttccgcgc tacgtcgtga agaccgacga ggtcgatcat ctcctgcacg cggcggcgtc
     2221 caataccgtg cgtctgtgcg agcgcgagca gatggttgta cgccgatcgg cccgtatgga
     2281 ccgagcgcgc ctcgagcagc gctccgacct cgtgcagcgg cgccgcgtgg tcgcggtagt
     2341 gcttaccgtt gacacgcacg tcaccggacg tcggagcatc cagcccgagg atcagccgca
     2401 tcgtcgtcga cttcccggag ccgttcggcc cgagaaagcc agtcacgatg cccggacgaa
     2461 cctcgaacga gatgtcggcc acggccgcct tctcaccgta gtgcttggtc aggttcgtcg
     2521 cttcgatcat gaagctaacg gtaggcgcgc ggtgccgccc ttcgcctcgg ccgcaagaac
     2581 gaacttcgcc tcctacaaaa gtcggaactg ccttacgcga gtcgtcggta gatcccgtcg
     2641 ccttccgagg ccaggccggc gacatcgagc tcggcgaggg ccgccatcac ctccccgaca
     2701 cctagccctg tcttctgcac gagctcgtcc gcggttgcgg gcagaaggtc caagagtgga
     2761 ttcccttcca ccgttcggtt cggtggcgat tggattccga acgattcgag gacgtcagct
     2821 gccgcagtca gcggagtcgc gccgagccgc aagagtgcgt tcgtcccggc ggacaacgtg
     2881 gatgtgatct caccggggac tacgaacacc tctctgccct cctcgagggc gaagtcggcg
     2941 gtgatcagcg cgccgctccg ctcgcgcgcc tcgacgacga cggtcacagc gcagaggccg
     3001 gcgatgattc tgttccgtgc cgggaagcgc cacggtgcag gctccacccc ggcctcgtac
     3061 tccgacacga tgagcgatcg ctcgccgatc gaacgcgcga gcatcgtgtg cgcggccgga
     3121 tagtcgcggt cgatgccgca gccgagcacc gcgaccgtgt ggccctcggt ctccagcgcg
     3181 ccgcgatgcg cctcgccgtc gatccctcgc gcgagcccgc tcacgaccac gacatcggcg
     3241 ccggcgagct cgcggccgag catgcgcgcg acctgggcgc cgtagggtga gcacgcgcgt
     3301 gcgcccacga ttgcgactgc tggctccgcg agcagcgtca cgtcaccgtt tcctctgaga
     3361 tagagccggg ccggtggatc gtggattgcg cgcagcagcg gcggcagctc gcgcctgcgc
     3421 aagactctca tggcgttgcg agctcggagg gagcgcgata ggcgagcgcc tcggcgacgt
     3481 gctccggcaa gactgcgtcg gagccggcca gcgcggcgat cgtgcgcgag acgccggcga
     3541 ccttcgcgcg gccccgcccc gagagcggta gccgctcgac ggcccgcgtc agcagctcgt
     3601 cggcggcgga agtccgcttg ggcgcagtct cctcgagccg caggcgcgcc gcgacgacac
     3661 gggaacggac gggatccgat ctctccccgg ggccgccttg cagctcaagc gcgcgggcgc
     3721 gcggaatcgt gacgaccagg tcgaagcgat ctagcagcgc ccgcgagagc ttgtcccgat
     3781 agcgcgcgag tctctgtgcc gagcagctgc actgcgcacc cggatccccg cgtgctccac
     3841 acggacacag gttcatcgtt ccgacgagct ggaaccgagc cggaaagcgg acgctgccgg
     3901 ccgcacgagc gacgacgagc tcgccgtcct cgagcggctg gcgcagcgcc tcgagggcgg
     3961 gacgctggaa ctccgccagc tcgtcgagca gcaacacgcc tcgatgcgca aggctcgcct
     4021 cgcccggcct caatctaggt ccgccaccga cgatcgcagc cgtcgacgcg ctgtgatggg
     4081 gcgcgcgaaa cggcgcgtac ccgatcaatg gatggtcggg gtcgacgaga cccgcgaccg
     4141 aatggattcg cgtcacctcg agcgcctgct cctcctcgag cggcggcagg atccctggca
     4201 gcctgcgggc gagcatcgtc ttgcccgtgc cgggcgggcc gccgagcagc aggttgtgac
     4261 ggccggcagc tgccaactcg agcgcgcgtc gtccgcgttc ctgcccccga acatccgcga
     4321 gatcgagcgg gcgccgacgc ggcttgccgt tgcccacgac gtactccggc ggctcgagct
     4381 ctccgcggag gtacgcgacc gcatccgcga gatggcgcat tccgatcggc tcgacgcctg
     4441 ccagggcagc ctcggcacag gcttcgtatg gacagatgat gcgctccagc tcggcacgac
     4501 gcgcgccctc ggcgacggca aggacgccgc ccacgcgacg caggcgcccg tcgagcgcga
     4561 gctcaccgca cgaagcgtgc tccgcgaggc ggtcgcgtgg aagctgatgc gacgctgcca
     4621 gcagcgccag ggcgatcggc aggtcgaatc cggagccttc cttcttaagt tcagccggtg
     4681 cgaggttgac cgtgacgtga ccccccggga actccagctc cgctgagagg attccactcc
     4741 gtacccgctg cctcgcctcc tggcaggcac ggtcggcaag cccgacgatc gtgagcgaag
     4801 gcgtcccgcc ctggagatgc gcctccacct ccacccgccg cggctcgagg ccgacgaggg
     4861 caaaggtgac cgcgcgcgcg agcacgctcc gaggctacgc agacaactgt gacgtctgtg
     4921 tatcgagtca gcgcgcgatc agcacgtctt cgaagtactc gccggcgcgg cgtccggtcc
     4981 ggcgattcac gtgacggcgc agacgttctt cgagccgaag gcctgcgcgt tccaggatct
     5041 ccgggtagag ctcgcggcgg tcgttgacga cgatcacgat cggtgcgcct cgccgcagca
     5101 cggacttcgt gttgagcaac acctcgacga tcccgtcgga gtaggcagcc agagccgcgc
     5161 gcgagttccc cgcggccgcc gcgccgacct cgagctcgcg gcgatcgtcg agaccgagca
     5221 gctcgtacgc gtagcgatgc tgctcgtggt agtcgatcag ccctgggtac ggcggcgagg
     5281 tgagcacccc gtcgtagcgg ccgggcaact gctcctcgcg cacatcgccg tggatgatcg
     5341 aggcgcagcg gccgcgtgcg cggacgcgcg agaactcctt cagccgtgcc agcgtgtcga
     5401 gcgtgtagcg gcgcaggaac tgcgacgccg tctcgacggg ccggcactcc cgccgatgct
     5461 tgtgacacca gtagggctcg agctgcggct cacgcggaaa ttcgagatcg aagtgggtcg
     5521 tgcgacgcgc cgatctcgcc gcgcgggcga ggacgacgcg gagcacgtct gcgtgctcgt
     5581 agtcgtcgat cagcgcgcgg aagccgagca gctcgcgtgc ggcctgcggc gcgtaccagc
     5641 ggcgaatgta ggccgccgga cgctcgactg ccgtctcgcc gacgcgtgcg tgtaccgctc
     5701 gcagctcctg ctccaggacg aacgggttgt acggacgagt cttgacgtcg atgagcaaac
     5761 agttgaacgc cgcgacgtcc acgccggtgg cgtcgaggcc gctctcgagc gcctgtacga
     5821 gcgtcgttcc cgaaccggcg aacggatcga gcacgtggtg gcccggccgg agataccttc
     5881 ccagcaggat ctccaccagc tgcgggatga acttgcccag atacggatga agccggtgca
     5941 cgtgcttcgt ccgctcccgt tctgggaggt cacgctcgga ccaggagagg gccagatcga
     6001 gctcggcgta gagggcgtcc tgcgtcgggg ccacccgcgc cagttcgacg ccggggtcaa
     6061 aaagcctggg ccacccgctc cagttggccg ctgcggacgg cgatcacgtc gaaagacacc
     6121 tgtagcccgg tcgtctccgg atggcgtgcg agccaggcgt ccgcggccag ccggattcgg
     6181 cgctgcttct cgtcatccac catctcgagc gggtcgccga accgctcgcc cagcttctcc
     6241 ttcacctcgc agaagatcac ccgccggccc cggcgaacga ctatgtcgag ctcgtagccg
     6301 gcgacccagg cgttcgtcgc caggattctg tacccgcgca ggcggtacca ccacacggcg
     6361 cgctgctcgg cgcgcttgcc gggacgcaca gacacgacgg gaggctacgc cgcgcggctg
     6421 cgcatgtctg tcacggatct cgaacggcat tcaccacgtg cagattgttc cgatcgacca
     6481 ccgccaacgt gcggccacgg ggccctcgca cgaccagacg accgtcaccg gcgcggcgga
     6541 tccggaactc gaagaggcgt ttcggttggc tcttccacgg gaggtcgata cagacggcgc
     6601 ccgcgtgcat ggtgtcgagc gcgtggatga acgtcttgtc cgggccgccg tacagcgtgt
     6661 aagcccaggt tcggtcctgc gacgtcacgc gcgagatcgg cgcgccctgc atcgccgttt
     6721 ccgactcgcg cttgtcgatg accgcccgcg cgagcaggcg gcctgcatgc aggtcatatg
     6781 ctcgcacgcg atagcgattg aagtcctccg cggagacgta ctcgatgaag tagaggtagc
     6841 gcccatccgg cgagagcgca tcgaagacct ggtgccctgg cagcctcagc ttccgcacca
     6901 gcttcatgct cttcaagtcg accagcgcga acgtgctgtg cttccggagc gccggtcccg
     6961 tagtcggctc gccgagcagc agcgtgcggc cgtctcccat caaaccgtca gcagtgccgt
     7021 cgaacgcgac gacggggatt ccccaggtgc ctttgagcgc catgaacttc agcacgcgac
     7081 cgtcctgacg gcgaatcact tctagagctg tcgagccggc agcgggcacc gcgacgtacc
     7141 ggaccgcccc gttggccagc ccgtcccaac cctgctgaac acccgcaccg ggcccccctc
     7201 catctgcgtt cgccgccgtc gcggcacagc cgatgcagat tgctgccgtc gccagcaaac
     7261 gtctaggcat cttcggcctc ccgtcgtcgc tcaatgacgt gatacgcctg gggccggtgt
     7321 ttgggtccgc taggcctgct tcgtcagaag cgcatggatc tcacgggtca acccagcgac
     7381 ctgctcggtc agctctttgt cctgcttcgt cagatccgtg ttcgcctcga gcagcgcctc
     7441 gagcttgtcc gtctccctct tcagtcgagc aacgcgttgc tcggcgttgt gctcggtctt
     7501 ctcgcggtgc gtttcgagct tttcgcccat caccttgtcg cgcgaagcct gtctcgtctg
     7561 ggccagcaag atcagcggtg cggcatacgc ggcctgggtc gagaaggcaa ggttcaggag
     7621 aatgaacggg tagggatccc aatgcgccat gagcgcgacc gagttgagcg cgatccaggc
     7681 gatgacgatg agcgtctgcc cgatgatgta ttgcggcgtt ccgaagaagc gcgcagcacg
     7741 ttctgccgca cggccgaaag gatcgtcgcc gaaggcactg tgctcggaca cgacaacgag
     7801 agtaggtcgt caggcggaca gtgccgccag ctcctcctct ttcaggtaac acatcgcctg
     7861 gaaggaacgt cggtgaatgc gtgacggccc acgctcgcgc acgatcctcg aatggccggg
     7921 cgtgatgtac cccacgtgcg acgagaagcc gtaatgcggg aagagcgcgt ccatccgccg
     7981 catcagacgg tcacgcgtca ccttggcgac gatcgaggcc gcggcgatcg cggcgctctt
     8041 ctcgtcaccg tcgacgatcg ccctgtgggg aggcgccgtc gggcctaggc ggaagccgtc
     8101 gacgagacat gcttcggccg gcggcgacag cgtgtggagg atctcgcgca tcgcggcgag
     8161 attcgatttg tgcagaccgt tgcgatcgat cgcgccatgc ggaatcaccc gcaccacaac
     8221 ctgctccgcg caccccagca ccgcgtggaa cagctcttcg cgacgctccg gcgagcactg
     8281 cttcgagtcg ttgagatacc cgagcggtcg caccttgtgg tcgcgcaggc agtcgtagtc
     8341 gagcagcacg ccggccacca ccaggggccc cgccaggcag ccacgaccgg cctcgtcggt
     8401 tcccgcgacc agccgggccc cgagctgccg gtcgaacttc aggagcttct ggccggtgcg
     8461 aggcgccacg gcgccgagga tagccgtcta ctcggcgggc tctgccgggg tgtcggcgga
     8521 aacgttgctt tcagcgactt ctacgggagg cgcgcccttg ggcgcgcgga cgtcctggcg
     8581 caactcgcgg atgcgagcct tcttgccgac cttcccgcgg aggtagtaga gcttcgcgcg
     8641 gttgacgtcg ccgaccgcgg cgacctcgat cttctcgatc ttcggcgtgt ggagcgggaa
     8701 cgtgcgctcg acgccgacgc cgaaggactg cttgcggacg gtgaacgtct cgcgggcgcc
     8761 ggcgccctgg cgcttgatca cgatgccctc gaagacctga atgcgctgcc gctggccctc
     8821 gatcaccttg aaatggacac ggaccgtgtc ccccgctttg aacttcggga tgtcaccgcg
     8881 caactgggcg cgctcgagtg cttcgatcac cttgctcata aggggacggc ccgaaggccg
     8941 gggcggaatc ctagcgaaag aacgacttag tggctcagag ccactaagtg cctagtggaa
     9001 ggagatccgg cccggtggcc agtacgtggc gaagaccttc ccgatcaggt tcttgcgcgg
     9061 cacgaggccc cagcggcgcg agtcgcagga ggagctccgg ttgtctccca tcatcaggta
     9121 gtagtccttc gggatccggg tgtaagtgtt cctgggcggg atgtcccgca gactgagggt
     9181 ctgggtgtcg cgccgatcgg ctttgacgta cggctcgttc agcttcttcc cgtcgatgta
     9241 gacgtagcct gctcgctcct cccacacgtc gccgggcaac ccgatcagcc gcttgacgaa
     9301 cgttccttcg gagccgcact cctgtttcgc caggctcggc gtcgtgaaca ccacgatctc
     9361 gccccgtttc gggttgcgga agcggtagat gaagcggttc gccagcaccc ggtccgaggt
     9421 ggccgcctcg cagccctggg ccggcctcgc gcagtgcagc gtcggctcca tcgacgacga
     9481 cgggatgcga tacggattca cgatccaggc cttgatcgcg aggacgatcg cgatggcgcc
     9541 cgcgatcgtc acgacccagt cgatcccgac ccgccagccg tggggaagat tcgggaagaa
     9601 gcggtcgatc gggttgtgcg acttcttctg cggagccgca ggcggcggcc cgaatccgtc
     9661 tcgctcggcg tcgtatctcg cgcgctcttc cggatcgcgc aggacgtcgt acgcgtctct
     9721 cagctcatgc caagcgccgt cctggtagcc gagctcgagc gcgccacgac ggtaggccga
     9781 ctcgatctcc tgcggcgacg cgtcggatcg cacaccgagc agcgcgtagt gatcgctcac
     9841 gtgacggtcc gccgccggct ctgttcccgt cgccattcct cgatctttcc gtggtcgccc
     9901 gaaagcagaa cctccggaac gtgccagccg cgaaactcgg agggacgcgt gtagtgcgga
     9961 tactccagac caccgtcgag ctcggaggag aagctctcga acgcacccga ctcctccttg
    10021 atcgccccgg gcagcctgcg cgcgatcgcg tcgacgagga ccatcgcggg caggtcgccg
    10081 ttcgacagca cgtagggccc gatcgagatc gcgtccgacg cgaggtgctg cacgacgcgc
    10141 tcgtcgaatc cctcgaagcg ggcggacagc agagtgagcc gctcctcgtc cgccagttcc
    10201 tccacgacgt cctgcgtcag ctgacggccc tgcggcgtca atgcgatcac acgccgatcg
    10261 ggcacgccgc cgtagacggt ctcgagcgct gctgcgacga catcgacccg caggaccatg
    10321 cctgcgccgc cgccgtaagg ctcgtcgtcc acctgcgcgg cgcgaagcgg cgtggtgtcg
    10381 cggtacgaga agagccgcag gtcgagctcg gtgccgagga cgctggcgac cgggcgttgc
    10441 tccgtcagcc aggcgaaggc atgcggaacc agagtgaaga cgtcgagctg cacgggcgag
    10501 cgacccaggt tagctagccg ctccggaaaa gcccggagcg accgtgatcc ggccagcggc
    10561 aagatcgacc acgcgcacac agtcctccac catcggcagg gcgatcccgg agtcgagctc
    10621 gagcacgtcg ttcgccacgc ccggcaccac gttcgtgaca gtcccgagct cgtctccgcc
    10681 gtcctcctgg acgcgcaggc cgaccagttg gaacacgtag tactcgtcct cgcctgccgg
    10741 tgcgagcgct tcccgtggaa cctcgagctc cgctcctcgg ggcacgcgcc ggtcgaggcg
    10801 gatcacagga cgcccacgcg catgtctgcg ggcgacgacc ttggcgggct caccctcgac
    10861 gatcagctcg gcccctacct cgaagcgttc cggcgcgtcg ctcgcgttct cgacgacgaa
    10921 cgagccgtcg agaccgtggg acttgccgac acgcccgacg acgacggtct gagacgcgct
    10981 cacgggaccg ccgctcgtct agtcgacgat ctcgagctgc acgcggcgat gctcgcgcac
    11041 gctgcccgca cggacgatcg tgcgcaacgc acgcgcaatg cgtccctgcc ggccgatcac
    11101 cttgccgaga tcgtcttgcg ccacgtgcag gcgaagcacg actgcgtcgg cctcctcgac
    11161 tctctcgacg cgcacggcat ccggctcgtc gacgagctgc cgcgccagat actcgagcag
    11221 ctccgccacc gttaccggtt caggttcctg atcttgagca gcttgctcac tgcttccgac
    11281 ggcaaggcgc ccttgccgag ccagtccttc acccggtcct cgtcgaactc gatcagggat
    11341 ggatcgtgtt gcgggttgta gcgcccgacg atctcgatga acttgccgtc acgcggtgag
    11401 cgcgagtcgg cgaccacgac gcgatacacc ggattcttct tcgatccgac tcgcgtcaac
    11461 ctcatgcgta cggccaaatt tacctcctgg atttctgtcg cttcttgcgt ttgttggatt
    11521 ttgtcgcttt tcgttgtccc ggctgcgggg ttgccgccgg cgcctgtccg gtcagggcac
    11581 cgagatccgg cagcttgcct ttgctcatac ccttcatgag cttctccatc tgcttgcgcg
    11641 cgtccagcag acggttcact tcctccaccg tcgttcccga tccgttcgcg atgcgtaggc
    11701 gccgcgagcc gtcgatcacc ttcggcagtc ctcgctcctg cggggtcatc gacaggatga
    11761 tcgcctcgac gcgacccatt tgcttctcgt cgacctcgtc cattccctcc agctgcttgc
    11821 cgaggcccgg gatcatcttc agcacgccct ggatcggtcc catgcggcgc agcatcttgt
    11881 agctcttcag gaaatcgtcg aaggtgaact ggcccttcat catccgggcc tcgaggtcct
    11941 tctgctcgtc ctcctcgacc gcttcctcgg cgcgctcgat gagcgtcagg acgtcgccca
    12001 tgccgaggat gcgggatgcc atccggtcgg gatggaagta ctcgagctgg tcgagcttct
    12061 cgccgacgga gatcagcttg atcggacggc cggtgacggc cttcaccgag agcgccgcgc
    12121 cgccgcgcgc atcgccgtcg agcttcgtca tcacgacgcc gtcgaacgcg atccgctcct
    12181 ggaacgcgag tgcgacgttc acggcctcct gcccggtcat cgcgtcgacg acgagcagca
    12241 cgttggcggg cttcgcctcg tcgcggacgc gctcgagctc ctgcatcagc tcctcgtcca
    12301 cgtgcaggcg tcccgcggtg tcgaggatga tcgtgtcgac accgtcgtcg gagagctgcc
    12361 ggaggccgtc gcgcacgacg gcgacgggat ccgtgccttc gccgcgatag accggaacct
    12421 ggatctgcgt gccgagctgc tcgagctggt cgatcgcggc gggcctctgc aggtcggcgg
    12481 cgacgagccc cgggctgcgg ccttccttgc gcagcatgag cgcaagcttc gcggcggccg
    12541 tcgtcttgcc cgatccctgc agaccggcga gcaggatcac cgtcggcggc cggcctgtga
    12601 atgcgagttg tgagctgccg gagcccatga cggcagtcag ctcgtcgtgg acgaccttga
    12661 cgacctcgaa gttgacgtcg gcctcgagga gcgcgagacg gatctcgcgc atggcacgcg
    12721 agatcgcctc atcgtcgagc tttccgcgcc cgcgcaggtc gccgagcgca ccctggagcc
    12781 tgtctgaaag ggtgtcgaac acggccggga agtgtacttg cgggtgtttc tgggccagac
    12841 ttgggccgtg cccgaccatt ccgagcgcaa gctcgccacg gtcatgttcg cggatctcgt
    12901 cgactcgacc gcggccgcgg gcgatcagga tcccgagcgg actcgggccc ggctggaacg
    12961 gttttacgac gcgatgagcg cggagatcga gggagccgga ggaaccgtcg agaagttcgc
    13021 cggggacgcg gtgatggccg cgttcggggc gccggaggcg ctcgaggacc acgccgagcg
    13081 cgccctgcac accgcgctgg cgatgcagcg gcgactcgaa gccgtctttg caggcgacct
    13141 gtccctgcgg atcggcgtca acacgggcga agtggtcgcg gggcggcccc gtgaggggag
    13201 ctcattcgtc agcggggacg ctgtcaatgt tgccgcgcgc ctcgagcagg cggccgagcc
    13261 gggcgagatc ctcgccgggg agcgaacggt ctccgccgtg cggggagcgt tcgaattcgg
    13321 cgagccgaag accgtcgagg cgaaaggcaa aaccggcggc gtcgcctgcc ggcccttggt
    13381 gcgcgggctc tcgctcatgc gcacgcgcgg agtgggcggg ctcgcgcgcg cgttcgtcgg
    13441 acgggacgaa cagctcgagc tacttcaatc cgcgtaccgc cgcgccgtgg agggatcgca
    13501 cccagtcctc gtcacgatcg tcggcgatcc cggcgtcggc aagacacgcc tcgtgcgcga
    13561 gctgtgggag cagcttgcag ac
//
LOCUS       DASVEU010000061         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1209555,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000061 DASVEU010000000
VERSION     DASVEU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209555"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..350
                     /locus_tag="VF225_02465"
     CDS             195..350
                     /locus_tag="VF225_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144147.1"
                     /translation="MKRIDCVCGHVVEAEDDDRLWEDAQEHLRTDHPDLVGKVSREDI
                     LAQAEET"
     gene            complement(347..1114)
                     /locus_tag="VF225_02470"
     CDS             complement(347..1114)
                     /locus_tag="VF225_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7144148.1"
                     /translation="MTQPGGTRVQLCGRLVVRLEGQRVEDQLPGAKGRLLLAYLVLNR
                     ARRMNRDELLNAVYGDDVSPDHQPRLTVLLSKLRRVLGPGLVTGRSEIEVVLARDAFV
                     DVEAAREGLHRAEYHVANEEWAEAWGPAGIAYHIANRPLLHGQDRSWLDEWRRRLDDI
                     RLRGLECFAQARLGLGGPTLPQAETCGRRLIELAPYRESGHQILMEALARRGNAAEAL
                     LVYDRLRILLRDELGIAPSPAVQSVYRRLLDQTTTTA"
     gene            1230..1805
                     /locus_tag="VF225_02475"
     CDS             1230..1805
                     /locus_tag="VF225_02475"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7144149.1"
                     /translation="MNVTTDTIRNGVDAEKMFATLDLIKAQPELAKFQFRATNRWVDE
                     AHNRSTIKGFYGAGAEDSTRIEEFQLNAGEPAILLGSNSGPNPVEYLLHALAACLTTS
                     IVYVATARKVELGSVESTLTGELDARGAFGLDDEPRNGFERITVSFRVTGDATEEKLR
                     EVVERARERSAVYDMVTNGVPVALEVTTHAD"
     gene            1795..>2052
                     /locus_tag="VF225_02480"
     CDS             1795..>2052
                     /locus_tag="VF225_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7144150.1"
                     /translation="MPIELAARTDAGARLVAIAEDLSAGLAVHAAEHDRDGSYPFEAI
                     EALKTSGYFRAPVPVELGGLGVSSAHDLVVASSRLGRGDASV"
BASE COUNT          359 a          698 c          688 g          307 t
ORIGIN      
        1 ggctgcgtcc acggccacgg cgaggaggac gtgggtaaga cggactgagc gaagcttcga
       61 aaccaaggcc gtctccgtga cccgcttcct ctcgatttgg accactgggc gggagaggaa
      121 acggtgaagc cccgcgagta ctggtcttcc accataaccc cgatgtggcg aaggcagcgg
      181 cttacactga gttcatgaag cggatcgact gcgtctgcgg tcacgtcgtt gaggctgagg
      241 acgatgaccg actatgggag gacgcgcagg agcacctacg gactgaccac ccggatctag
      301 tcggcaaggt ctcccgcgag gacatcctcg cgcaggcaga agagacctag gccgtcgtcg
      361 tcgtctggtc gagcaggcgt cggtagacgc tctgcacggc cgggctgggc gcgattccga
      421 gctcgtcgcg aagcaggatc cgaaggcggt cgtagacgag tagcgcctcg gcagcatttc
      481 cgcgccgcgc aagcgcctcc atcaggatct ggtgaccgct ctcacggtag ggcgccaact
      541 cgatgagcct ccgcccacac gtctcggcct gcggcagcgt tggtccgccg aggccgaggc
      601 gcgcctgggc gaagcattcg agtccgcgca accggatgtc gtcgaggcgt cgtcgccact
      661 cgtcgagcca tgatcggtcc tgaccatgga gcaggggccg gttggcgatg tggtacgcga
      721 tgccggcagg gccccaggcc tcagcccact cttcgttggc gacgtggtac tcggcgcggt
      781 gcaggccctc acgcgcggcc tcaacgtcga cgaaagcatc gcgcgccaga acgacctcga
      841 tctcggatcg tccggtcacc aatcccggcc cgagcacgcg acgcagcttc gagagcagca
      901 cggtcaaccg cggctggtgg tcgggtgata cgtcgtcgcc gtagaccgcg ttcaacagtt
      961 cgtcccgatt catccgccgg gcgcgattga gaacgaggta ggccagtagc agccgcccct
     1021 tggcgcccgg cagctgatcc tccactcgct gcccctccag cctcacgacc agccgtccgc
     1081 agagctggac tctcgtacca cccggctgcg tcatccccag caagcttaga aagctctacg
     1141 caaggttcgg gaaaccagcc gctgggacca tcgctcgcga acgaagcaag cccttctacc
     1201 aatcgcacga cgacccagga ggcctcgcag tgaacgtaac gacagacacc atccgcaacg
     1261 gggtcgacgc ggagaagatg ttcgccacgc tggacctgat caaggcgcag cccgagctgg
     1321 cgaagttcca gttccgcgcc accaaccgct gggtcgacga ggcccacaac cgctcgacga
     1381 tcaagggctt ctacggcgcg ggcgccgagg acagcacgcg catcgaggag ttccagctca
     1441 acgccggcga gccggcgatc ctgctcggca gcaacagcgg gccgaacccg gtcgagtatc
     1501 tgctgcacgc gctggccgcc tgcctgacta cctcgatcgt ctacgtggcg acggcgcgca
     1561 aggtggagct gggttcggtc gagtcgaccc tgaccggcga gctcgacgcg cgcggcgcat
     1621 tcgggctgga cgacgagccg cgcaacgggt tcgagcgcat caccgtgtcg ttccgcgtga
     1681 caggcgacgc cacggaggag aagctgcgcg aggtcgtgga gcgggcgcgg gagcgctccg
     1741 ccgtctacga catggtcacg aacggcgtgc ccgttgccct ggaggtcacg acccatgccg
     1801 attgagctcg ctgcccgtac cgacgccggg gcgcgtctcg tggcgatagc ggaggacctc
     1861 tccgcggggc tcgccgtcca cgcggccgag cacgaccgcg acggcagcta ccccttcgag
     1921 gcgatcgagg cgctgaagac ctccggctat ttcagggcgc ccgtccccgt cgagctgggc
     1981 gggctcggcg tgtcgtccgc gcacgacctt gtcgtcgctt cgagccgcct ggggcgcggc
     2041 gacgcctccg tc
//
LOCUS       DASVEU010000062         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_821985,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000062 DASVEU010000000
VERSION     DASVEU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821985"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /gene="yidD"
                     /locus_tag="VF225_02485"
     CDS             complement(<1..202)
                     /gene="yidD"
                     /locus_tag="VF225_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7144151.1"
                     /translation="MKRARGLSWIVGAPARGLLIGLIRLYRLTLSGWLGGQCRFYPTC
                     SRYAEDAIRTHGAVRGTALSAWR"
     gene            complement(199..492)
                     /locus_tag="VF225_02490"
                     /pseudo
     CDS             complement(199..492)
                     /locus_tag="VF225_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            complement(551..688)
                     /gene="rpmH"
                     /locus_tag="VF225_02495"
     CDS             complement(551..688)
                     /gene="rpmH"
                     /locus_tag="VF225_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7144152.1"
                     /translation="MSKRTYQPNNRRRAKRHGFRLRMRTRAGRAIVARRRAKGRSRLS
                     A"
     gene            945..2333
                     /gene="dnaA"
                     /locus_tag="VF225_02500"
     CDS             945..2333
                     /gene="dnaA"
                     /locus_tag="VF225_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7144153.1"
                     /translation="MAVASFEEAAESTWSRVVERARSELSESSFSMWFQDVRATRLRD
                     GVLDVVAPSEYVKDRLAKNYADLIRTAATEALGLPVRVRFRAAPPEDAALQGDADPSP
                     PPPDRSSSDTSRPAVTPGLPFPNYTFEAFVPGPSNRFAHAAGMAVAEAPPSKAYNPLF
                     IYGGVGLGKTHLLVAIGHQMHRLSPRLRVKYVTSESFMAEFIKAVRERQGYQFAARYR
                     DVDVMLVDDIQFLAKREETQTEFFHTFNALHEKERQIVIASDRPPQELGMEERLQSRF
                     RLGLCVDVQPPDLETRIAILQLKAQREAVHLPDDVIDFIASKFDQNIRELEGALVRVV
                     AWSDLTGHAITIELAEHALEDLLPQSEAEIPPQLILEESARYFGLSVHDLVSPNRSRP
                     LTTARHVAMYIMRECSGQSLVKIGDTFGGRDHTTARHGILKIERDMRARSTTYRQVQD
                     LSRIIRGRARAG"
BASE COUNT          422 a          940 c          773 g          372 t
ORIGIN      
        1 gcctccacgc cgagagcgcc gtcccccgga cggccccgtg cgttcggatc gcgtcctcgg
       61 cgtacctcga gcaggtcggg tagaagcggc actggccgcc gagccatccc gacagcgtga
      121 gccgatacag gcgtatcagc ccgatcagca gcccgcgcgc cggcgcgccc acgatccacg
      181 aaagcccacg agcgcgcttc acgaacgagc cacccgcgcg cgagagagca gcttctccac
      241 ctccgtcacc agctcgtgcg ttcgtgcccg gtggatgctt gctcgggcga ccagtgcgat
      301 gtcgtatccc tccaggaccg tcggctcgag ctcccgccaa gcggcccgaa ggatccgccg
      361 ggcccggtta cgcaagaccg cgcccccgac gcggcgtccg gccacgaacg ccaccgtccc
      421 cgagccggga gcgacaaaca ctaccacccg ctctccgtgg aacgcgcggc ccgttgccag
      481 gatgcgaccg acctctgacc tggtcgtggc gctcgtgacc tgcgctcgcg ccggctacgc
      541 gcccgtgtcg ttacgcggac agacgggaac ggcccttggc acgccggcgc gcgacgatcg
      601 cgcgccccgc acgcgtccgc atccgaaggc ggaacccgtg gcgcttggcc cgtcgacgat
      661 tgttcggctg gtacgtccgc ttcgacatcc catgtccttc ggtaaagatg gctcgagttg
      721 caacgcgccc ctctgcaggt caccaagtat agccgccggt cttgcgaacg ccgtgtgaac
      781 acttggcggc cagaaagtgc gtcaaaagca cccggcagac gggcattggg aaaaacggcg
      841 gtgctatagt ccggcgcgct tcacctcggc gcccgcatcc ccgcagggcg tcttgccctt
      901 ccccaagggt ttccacactt gtggaaaggc actgtggaca tcgtatggcc gtcgcgtcgt
      961 tcgaagaagc cgccgagagc acctggtccc gggtcgtcga gcgcgctcgc agcgagctct
     1021 cggagagctc gttctcgatg tggttccagg acgtgcgcgc cacccgcctg cgcgacggcg
     1081 tcctcgacgt ggtcgcgccc tcggagtacg tcaaggaccg gctcgccaag aactacgccg
     1141 acctgatccg caccgcggcc accgaggccc tggggctccc ggtacgcgtc cgcttccgcg
     1201 cggctcctcc cgaagacgct gcgttgcaag gcgatgccga tccctcaccg ccgccacccg
     1261 accgatcctc gagcgatacc tcgcgcccgg ccgtcacgcc ggggctcccg ttccccaact
     1321 acaccttcga ggcgttcgtc cccgggccgt cgaaccggtt cgcccacgcg gcgggcatgg
     1381 cggtcgccga ggcgcctccc tccaaggcct acaacccgct cttcatctac ggcggcgtcg
     1441 gcctcggcaa gacacacctg ctcgtcgcca tcggccacca gatgcaccga ctctccccgc
     1501 gactccgcgt gaagtacgtg acgagcgaga gcttcatggc ggagttcatc aaggccgtcc
     1561 gcgaacgtca gggctaccag ttcgcggcgc gctatcgcga cgtggacgtc atgctcgtcg
     1621 acgacatcca gttcctcgcc aagcgcgagg agacccagac cgagttcttc cacacgttca
     1681 acgcgctgca cgagaaggag cgtcagatcg tgatcgcgtc ggaccggccc ccgcaggagc
     1741 tcggcatgga ggagcgtctg cagtcgcggt tccgcctcgg gctctgcgtc gacgttcagc
     1801 cacccgacct cgagactcgg atcgcgatcc tgcagctcaa ggcgcagcgc gaggccgtgc
     1861 atcttcccga cgacgtcatc gacttcatcg ccagcaagtt cgaccagaac atccgcgagc
     1921 tcgagggcgc gctcgtgcgc gtggtcgcct ggtccgatct caccggccac gcgatcacca
     1981 tcgagctcgc ggagcacgcc ctcgaggacc tcctgccgca gtccgaagcc gagatccccc
     2041 cgcagctcat cctcgaggag agcgccaggt acttcggcct gtcggtccac gacctggtgt
     2101 ccccgaaccg ctcgcgcccg ctcacgacgg cgcgacacgt cgccatgtac atcatgcgcg
     2161 agtgcagcgg ccagagcctg gtcaagatcg gggacacgtt cggcggcagg gaccacacca
     2221 cggcgcgaca cggcatcttg aagatcgagc gcgacatgcg cgccaggagc accacgtacc
     2281 ggcaggtcca ggacctctcc cgcatcatcc gcggcagggc ccgcgcggga tgatcggcgt
     2341 ccacggacaa cgtgtgcgga acccgggagg taacggggac gccggtcggc gcccatcgga
     2401 tcggtgcacc gattccccca acgggtcccc atgtcacacg ggcccactac gctcggaaaa
     2461 cgaggcggtt tccccctttt ccacagcccc tactactact gaaagag
//
LOCUS       DASVEU010000063        15780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_435371,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000063 DASVEU010000000
VERSION     DASVEU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435371"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VF225_02505"
     CDS             <1..694
                     /locus_tag="VF225_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7144154.1"
                     /translation="AEIQDVAAIVRRAHEVGAHVVLDAYQSAGIVPLDVTALNVDFAV
                     GGSVKWLCGGPGNGWLYVRPDLADVLEPTFVGWQAHARPFGFEPELDYAEGAARFLTG
                     TPNVPALYAATAGYDLIEEIGVDRIRENSVRQTQLLIDLFDEAGFDVGSPRDSARRGG
                     TVTVRTPEFEAVHKELSERQILCDFRPDAGLRLGPHYYNTDDELRHAVARIAEILETG
                     AYERHLGATARF"
     gene            755..1648
                     /locus_tag="VF225_02510"
     CDS             755..1648
                     /locus_tag="VF225_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144155.1"
                     /translation="MKVTFLIAAALAFISAAGAARASEIIDRNAQSVRLGVDASGHAM
                     VTYKVRGRVMHVRAWGAIDARQPTTSRPQVRLRKDYSGRNWTSFKNQCRAYDGPRLAF
                     QIAACAAPDGSYWALQSWRRTLPNFDGPRRGGLGAWELHLSHWSGAIATLEAWTDWVY
                     DGRYHHLFGRLTYDGRPVYGFSATRVGSPLDTYGRNIYVDTLDSRYGKGWRRENAFLS
                     HRPTGVFCYGFYGFTSRGPGNGAKYRLTAIGPGVTPDVSVTLPGLHDYNPNSPEDVAY
                     EQQRNALQDSIAGVDTKCRVH"
     gene            complement(1642..2274)
                     /locus_tag="VF225_02515"
     CDS             complement(1642..2274)
                     /locus_tag="VF225_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7144156.1"
                     /translation="MSELFVGTSGWSYPSWKPGFYPAGTDAKEFLRFYAARFSTVELN
                     TTGYRLPAEEQFARWAEQTPAGFTFAPKLAGNRPRLLAEFGRVRALGDRLGPIRVQLI
                     SARDEGMIELILGSLDPDLKLAFDLAHPSWDGIESRLAEASAVRINDLDHEAPFRYVR
                     FRDPPYDDEALQEWADRLRAVDVPLYAYFRHEDEPTAPAYALRLLELLGQ"
     gene            complement(2267..3163)
                     /locus_tag="VF225_02520"
     CDS             complement(2267..3163)
                     /locus_tag="VF225_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7144157.1"
                     /translation="MATPESSLVAPPADIAAHWGIELGASLEPLAPSRALVLRSGVYV
                     IRDAKTSLESVRWEHEFLGFLAPRVAEVVAPLTAVDGSTFLAVGDHVISVTPHIAAEL
                     ADRSAPVVRYEVPRLLARLHRAAAEWPRARPRPGRPSWRDLDWIANDTWDWTAIDRTA
                     LLERAYEASREWLASAPPLAESPVHGDFHTDNLLASERGIEGLIDWEFARIDWPASDL
                     AAAVMVLSLQRDGTIDQRIADETVAAYVDAGGRDESHALEPLLRQVLLAVALHARTRK
                     ATGQSWSPEFQGMLETALERFV"
     gene            3156..3863
                     /gene="thyX"
                     /locus_tag="VF225_02525"
     CDS             3156..3863
                     /gene="thyX"
                     /locus_tag="VF225_02525"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7144158.1"
                     /translation="MATTDRVTAEDAIRVLDHGFVRLDDAMATDLSVVNAARVSFARR
                     KDEMDDSDEGLIRFLMRDLHGTPFEHNSFRFHVRTPIFVAREWFRHRVGSFNEFSMRY
                     AKATDDFYIPEPEDVRSQVGKPGAYSFEPVDDELAERTREELRAVYEHAFETYERLVE
                     AGVARELARSVIPVGAYTEFFWTVNARALMNFVSLRAAETAQREIRRYANAVEQFFAA
                     KMPVTHAAFVERGRIAP"
     gene            complement(3860..4486)
                     /locus_tag="VF225_02530"
     CDS             complement(3860..4486)
                     /locus_tag="VF225_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144159.1"
                     /translation="MSRVPEFDDLVGMDVPEEERQRLRRAHELLVQAGPPPELSPAMD
                     AVPWPEESQRPAGKPRQQRLLLLAAALATAAVLGFVLGQSSGPSSSSASIDPVRVVTL
                     AGTSLDRNARGTLALGKADEGGNWPMLLHATGLRQLPEGGYYNLYLTKGGKPVVLCGS
                     FNVRSGEAIVRFSAAYDLSHFDRNGWVITRQLPSNHEPTQIVLKPTVA"
     gene            complement(4483..5127)
                     /locus_tag="VF225_02535"
     CDS             complement(4483..5127)
                     /locus_tag="VF225_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144160.1"
                     /translation="MKSRRNPEKMGISIALPAAMPHGRSRETTGATPLHERGLTDGDL
                     IRRVARRDADAFELLYQRYARPVFGLALRRLGDRMRAEDAVQETFAAIWRSAKTYRPE
                     RGAGAPWLYTVARNAIVDRSRNRTELPAEVPDTATVEPGPPERAETSYVAWRVHRALE
                     ELSGNERDVIELAYYAELSQSEIADFLGIPLGTVKTRTRAGLSRLADLLEGELQ"
     gene            5254..6132
                     /locus_tag="VF225_02540"
     CDS             5254..6132
                     /locus_tag="VF225_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7144161.1"
                     /translation="MRRFIPIAVAGMALVLAAPASTATTTVQIKSTGFVPATVTINQG
                     DSVTWTNTDTKDHQVVSNAGAFASAILKPGKSYTHAFPSGGTYRYHDGLHPSLKGAVT
                     VRGAPPQVTLATSAPVVKFGTQVTLTGTVNNKKTAETVTIAALPFGQTTKQVVATLQT
                     TSGGAFSFNVTPQLNTTYEAQWKGAASSVGVQVQPTVKLPFVSRSGYFHFYVTAGQSF
                     AGRFVYLQRFTLARTWINIRKLQLGQQSGRIMGMKFVRSVIPRGRWSIRIYMPASEMP
                     GGYIDSWSGTQPVVKR"
     gene            complement(6151..6759)
                     /locus_tag="VF225_02545"
     CDS             complement(6151..6759)
                     /locus_tag="VF225_02545"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7144162.1"
                     /translation="MPFEVPPLPYDYAALEPHIDEQTMHLHHDKHHQAYVDNANKALA
                     GTEWENQPVDHVLANIEIVPDDIRAAVRNNAGGHANHSLFWEIMSPNGGGEPSGSLAD
                     AINDTFDSLDELKKLMNDTGVKRFGSGWSWLVYDGTGLAVYSTANQDSPISNSDVPLL
                     GIDVWEHAYYLKYQNRRPEYLEAWWNVVNWDAVQQRLESADK"
     gene            6947..7567
                     /locus_tag="VF225_02550"
     CDS             6947..7567
                     /locus_tag="VF225_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7144163.1"
                     /translation="MTSLAFKFEEITARELKPELYELDGISRASIEAHYKLYQGYVNK
                     RNEILGRLDGVDTGSANQVYSDVRALKVDFSFAIGGIKNHEIYFEHLGGGGGDPTGLI
                     GDLIERDFGSVQNWRTDLKATGMAGRGWAWTAYDWDEGRLFNYVGDALNTYPIWNATP
                     LIALDVYEHAYFLDYQTDRASYIDAFFDNLDWLTVNDWVSKYQIQK"
     gene            7574..8218
                     /gene="plsY"
                     /locus_tag="VF225_02555"
     CDS             7574..8218
                     /gene="plsY"
                     /locus_tag="VF225_02555"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7144164.1"
                     /translation="MRTVLLVAAGYVLGSMPWGYWLPLLVKHDDVRRHGSGNIGGTNV
                     WRVYGWRLGLPVVLLDTLKGFAPALVATLMVSHLAGVLAGAAAMLGHWRPLFLRWQRG
                     GKVVATTGGAFLGVAPVVGAVGAGVWIVVFLAFRYASLASIVGALSLPVAAVLLGEPW
                     PVIAFAGGAAAAVLVLHRPNIARLRAGTENRFKFRRRRASAPASPDRVGTQSGR"
     gene            complement(8280..9251)
                     /gene="mptA"
                     /locus_tag="VF225_02560"
     CDS             complement(8280..9251)
                     /gene="mptA"
                     /locus_tag="VF225_02560"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HEX7144165.1"
                     /translation="MTESRELLDDLQASTPEVRLGLSRAGVQGVAKAIRLSWGGAEKL
                     ISAEIDCFVDLDPSQKGVHMSRFPELFEEAIDEVVIGEAFLVEELAEHIARHIVGRQR
                     AGRAEVRIAARYPLERRTPVTGLLTQEMISIVGIAAATESRVRRIVGVEATGINACPC
                     AQGLVRGKASERLLEAGFEEPDVERILELVPLATHNQRGRGTLYIGTDQTLNAEELAG
                     IVENSMSSPIYELLKRPDELFVVEHAHLQPRFVEDSVRISLRSTLDRYPDLKDEDFVL
                     ARQLNFETIHSHDVLGERYGTVGELRGELRDGEPAARHTELRDWLAS"
     gene            9265..9867
                     /locus_tag="VF225_02565"
     CDS             9265..9867
                     /locus_tag="VF225_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7144166.1"
                     /translation="MSDRSVVDSDASFPRLVSLAAHDLRTPLATIHGFAQTLVRMGDL
                     GEPKQRYLEMIDSAALQLAELLDELGVAARIEGGRYEPALQPADTLELARGAAEQLGE
                     ERVLVSGQGGEVRVDGEATQRGISALAQAALRHGGLEQIDLRVDSATLAISPVTPASG
                     PVLLGQELRDLGAAVAVIVIDAIGGSVALDGDTLTVKLPE"
     gene            9864..10067
                     /locus_tag="VF225_02570"
     CDS             9864..10067
                     /locus_tag="VF225_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144167.1"
                     /translation="MNRAAQVSLRRWLRRQLQQPTPGREHLEAAVEHNDPAEVRRLLA
                     GVPFTDAQRRHVEGLLDAWEEAR"
     gene            complement(10064..10543)
                     /locus_tag="VF225_02575"
     CDS             complement(10064..10543)
                     /locus_tag="VF225_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7144168.1"
                     /translation="MSDLLQALYQGDRVRVDELLAADPKLDVFEAAAFGRTDRLRELL
                     DEDPSRANEFGDDGFHPLGLACFFGHVEAARLLLDRGADVNALSTNEHVQTAAIHAGA
                     AAQGTDEATRYELVKLALEHGADPNLEQGGGFRAIDAARQNRDARIEQLLLENGAED"
     gene            complement(10543..11370)
                     /locus_tag="VF225_02580"
     CDS             complement(10543..11370)
                     /locus_tag="VF225_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7144169.1"
                     /translation="MPELPEMEAWRRALTDPVSAFPIAKAGPAHIATLKTFDPPLSAL
                     EGRQLAGADRRAKRLLFPTTDGELVVLVHLMTAGRLKYLKTGEAGPKTPAFQLVFEGG
                     ARLVLTEAGAKKRAGVWLLTPEEAEQELSHLGPEALGLGAERLAEICASESRRLHSLL
                     RDQRVIAGIGRAWANEILHHAQLSPYELTRDVTTEQVEQLANAIDTELERGLELREAG
                     ANDNRTYRVHKKLGEPCYVCGTPIAQVDFEEHTIYYCPECQTGGRILKDRRLSRLLR"
     gene            11418..12272
                     /locus_tag="VF225_02585"
     CDS             11418..12272
                     /locus_tag="VF225_02585"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7144170.1"
                     /translation="MRRGGVTEAVHRVHAVAVQDGAVIAEAGDTQLLAFMRSSSKPLQ
                     AIPLARARADLEERDLAIASASHLADDAQLTAVRALLAKAPATEDELECGPAGQPPSR
                     LKHNCSGKHAGMLALCRAKGWRSEGYRLEGHRAQREMLAVHAEASQILEDEIRTAIDG
                     CGVLTFGLPLERMANAFARLPELEGGDKVAAGMRAYPDLIRGPIASDTRLMKALPGWI
                     AKGGAEGLLCAAGSGIGVALKAEDGSGRGLGPAAAAFFRQLDLDLGDLAVVPLENSRG
                     ELVGEITI"
     gene            12419..13510
                     /gene="rpoD"
                     /locus_tag="VF225_02590"
     CDS             12419..13510
                     /gene="rpoD"
                     /locus_tag="VF225_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7144171.1"
                     /translation="MLTVEISLPEVEELQKLVQEGLEKGHLNYDEIAAGLDDVELTKE
                     QIEDFYTYLVDHGVELLEGETHKHPPHELAAPEEEKGPKLDLSVEPSLDSLRLYLREI
                     GKVSLLTADQEVYLAKRIERGDMSAKTQMIEANLRLVVSIAKSYLGRGLSFLDLIQEG
                     SLGLIRAVEKFDYRKGYKFSTYATWWIRQAVTRAIADKARTIRIPVHMVEKLNKVVHI
                     ERQLVQRLGREPRPDEIAEELEMTTDEVREILRMSQLPISLEKPIGEEEDSELGDFVQ
                     DESAESPFDTATLSLRREDVEHALSALPERERQVIELRFGLSGAQPCTLEEVGRAFGV
                     TRERIRQIENNTLKKLESLPEAQGLKDCV"
     gene            complement(13507..15633)
                     /locus_tag="VF225_02595"
     CDS             complement(13507..15633)
                     /locus_tag="VF225_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144172.1"
                     /translation="MVLLYARFGGHPSFKGCRGLADTDFVATDVAASRPGPEVSAPRA
                     FARVAAVPAWVWVAGIVLASFGGRLFAAVARLTPYYLPDEYIYPSLARSLAEHGRPLI
                     RGAGVHFPALLDPIVTAPVWLVTDDPLTAFRLTQGIHAVLFSLAAVPAYLLCRRLGLA
                     RWLGIAVAALTVAVPDGVYSSTMLADPLAYPLVLSAVYAGVCVVSQPTLRAQLAFSVF
                     STLAILARIQYVIVPLAVLTAELVADRFHVVQAVRRLWLALVLLIVPLALLLATLGSD
                     RVFGVYAHGNHALHPVSILHWVGLEAMLLVYSAGWAIVPGALAGLAVVLFRPRRRAEI
                     AFAVTSLLLVCGLLLEAAQIADTDSQRFQERYLFTLVPLLAVAFGLYVKRGLPGRVPV
                     GLLSAGLLLLAARVPLSGYAAAHNKDDSPTLWAVLRFEGLTSVANGSLVFALVAAVLS
                     VLVALVAFRKLPAAVAVVAAIVACCAMSAGASSFDARTSRSLRSTLPHDIRWVDHARL
                     GSVDLVAPPGSRKEQSWQQLFWNTSVKRLLLLGSAPIDQFDAKQLKIAGDGTLLVDGR
                     PDRRPLLVQTYASTVQLSGVERVRHELIFDLYRPVGTPRLRLLAAGRFVDKWLAPQGA
                     ITVWTKTGGTLELVLALPAHARVTPIVLTGRGIKRTIHVHPRQRIQLSFRVPAGGPWS
                     LHFSSARPGYLGDRAVSVRAELVRFR"
BASE COUNT         2687 a         5230 c         5370 g         2493 t
ORIGIN      
        1 ggcggagatc caggacgtcg ccgcgatcgt ccgtcgcgcg cacgaggtcg gtgcgcacgt
       61 cgtgctcgac gcataccagt ccgccggaat cgtgccgctc gacgtgacgg cgttgaacgt
      121 cgatttcgcg gtcggaggca gcgtcaagtg gctgtgcggc gggccgggca acgggtggct
      181 ctacgtccgg cccgacctcg ccgacgtgct ggagcccacc ttcgtcggct ggcaagcgca
      241 cgcacgtccc ttcggcttcg agcccgagct cgactacgcg gagggtgcgg cacgctttct
      301 caccggcacg ccgaacgtcc ctgcgctcta cgcggcgact gcgggctacg acctgatcga
      361 ggagatcggc gtcgatcgca tccgcgagaa ctcggtccgt cagacccaac tcctcatcga
      421 cctgttcgac gaagccggct tcgacgtcgg gagtccgcgc gactcggccc gccgtggcgg
      481 cacggttacc gtacgcacgc cggaattcga agccgtgcac aaggagctga gcgagcgcca
      541 gatcctctgc gacttccgtc ccgacgcggg acttcggctc gggccgcact actacaacac
      601 cgacgacgag ctccgccacg cggtcgcccg gatcgccgag atcctggaga ccggggcgta
      661 tgagcgccat ctgggcgcaa cagctcggtt ctgaggctcg gaacactcgt tcgagggagc
      721 ctgatcggga gggagggcgg gtactttgag actgatgaag gtcaccttcc tcatcgccgc
      781 agcactcgct ttcatctccg ccgccggtgc tgctcgcgcc tcggagatca tcgatcggaa
      841 cgcgcaaagc gtcaggctcg gagtcgacgc gagcggtcac gcaatggtca cctacaaggt
      901 ccgcggacgg gtcatgcacg tgcgcgcatg gggcgcaatc gacgcgcggc aaccgacgac
      961 gagccggccg caggtcaggc tccgcaagga ctattcgggc cggaattgga cctcgttcaa
     1021 aaaccagtgc cgcgcctacg acggaccgag gctggcgttc cagatcgctg cctgcgctgc
     1081 gcccgacggt tcctactggg cgctccaatc ctggcgtcgg acgcttccca acttcgacgg
     1141 ccctcggcgc ggcgggctgg gcgcctggga gcttcatctc tcccactggt cgggcgcgat
     1201 cgcgacgctg gaggcatgga ctgattgggt ctacgacggc cgctaccacc acctcttcgg
     1261 gcggctcacc tacgacggcc ggcccgtgta cggcttctcc gcgacgcgcg tcggctctcc
     1321 gctcgacacc tacggccgca acatctacgt cgacacgctc gattcgcgtt acgggaaggg
     1381 ctggcggcgg gagaacgcct ttctgtctca ccgaccgacc ggagtcttct gttacggctt
     1441 ctacggattc acctcacgcg ggcccggcaa cggcgcgaag taccggctga cggcgatcgg
     1501 gcccggcgtg actccggacg tgagcgtcac gctcccgggc ctacacgact acaacccgaa
     1561 cagccccgaa gacgtggcct acgaacagca gcggaacgcc ttgcaggact ccatcgccgg
     1621 agtcgacacg aagtgccgcg ttcactgacc gagcagctcc agcaaccgta aggcgtacgc
     1681 cggggccgtc ggctcgtcct cgtgccggaa gtacgcatac aggggcacgt cgacagcgcg
     1741 tagccggtcg gcccattcct gcaacgcctc gtcgtcatac ggcggatcgc ggaaccgcac
     1801 gtatcggaac ggcgcctcgt ggtcgaggtc gttgatccgc accgcgcttg cctccgcaag
     1861 ccgtgactcg atgccgtccc aggagggatg cgcgagatcg aaggccagct tcaggtcggg
     1921 gtcgagcgag ccgaggatca gctcgatcat cccctcgtct cgggcgctga tcaactggac
     1981 gcgaatcggc ccaagccggt ccccgagtgc acgcacgcgt ccgaactcgg ccaacaggcg
     2041 tggtcggttg cccgcgagct tcggcgcgaa cgtgaagccc gccggcgtct gctcggccca
     2101 gcgcgcaaac tgctcctcgg cgggcagccg gtagccggtg gtgttcagtt cgacggtcga
     2161 gaaacgcgcc gcgtagaagc ggaggaactc cttcgcgtcg gtcccggccg ggtagaaccc
     2221 cggcttccag gagggatacg accagccaga ggtgccgacg aaaagctcag acaaagcgct
     2281 ccagggcagt ctcgagcatt ccctggaact ccgggctcca actctgacca gtcgccttgc
     2341 gggttcgcgc gtggagtgcg acggcaagaa gaacctgccg caacaggggt tccagcgcgt
     2401 gcgattcgtc gcgtccaccg gcgtcgacat aggcggccac cgtctcgtcg gcgattcgtt
     2461 ggtcgatcgt cccgtcgcgc tgcagggaga ggaccatgac ggccgcggcg agatcggagg
     2521 ccggccagtc gattcgggcg aactcccagt cgatcaaacc ctcgattcca cgctcggatg
     2581 cgagcaggtt gtccgtatgg aagtctccgt ggacgggcga ctcggcgagc ggcggcgcgc
     2641 tcgcgagcca ctcacgcgac gcttcgtagg cacgctcgag gagagccgtt cggtcgatgg
     2701 cggtccagtc ccaggtgtcg ttggcgatcc agtcgaggtc gcgccaggac ggccgtcccg
     2761 ggcgcggccg cgctcgaggc cattcggccg cggctcggtg cagacgtgcg agcagccggg
     2821 gcacctcgta gcgcacaact ggggcacttc gatccgccag ctccgcggct atgtgcggcg
     2881 tgacggaaat cacgtgatca cccacggcga gaaatgtgct gccgtccacc gccgtcaaag
     2941 gcgccacgac ctcggccacc cgtggcgcca ggaagccgag gaattcgtgc tcccaccgga
     3001 cgctttcgag actggtcttt gcgtcgcgga tgacatagac gccgctccgc aagacgaggg
     3061 cgcggctcgg tgcgagcggc tcgagcgacg cgcctagttc gatgccccag tgcgcggcga
     3121 tgtccgccgg gggcgcgact aaactggatt cgggagtggc cacgaccgac agagtgacag
     3181 ccgaggacgc catccgtgtc ctcgaccacg gcttcgtcag gctcgacgac gccatggcta
     3241 ccgacctctc ggtcgtgaac gcggctcgcg tcagcttcgc ccgccgcaag gacgagatgg
     3301 acgactccga cgagggtctg atccgtttcc tgatgcgcga tctgcacggc acgccgttcg
     3361 agcacaactc gttccggttc catgtccgca cgccgatctt cgttgcgcgc gaatggtttc
     3421 gccaccgtgt tggctccttc aatgagtttt ccatgcgcta tgcgaaggcg accgacgact
     3481 tctatatccc ggagcctgaa gacgtgcgca gccaggtcgg caaacccggt gcctattcct
     3541 tcgagccggt ggacgacgag ctcgccgagc gaacgcgcga agagctacgg gcggtctacg
     3601 agcacgcctt cgagacgtac gagcgactcg tcgaagcagg cgtcgcgcgc gagctcgcgc
     3661 ggtctgtcat accggtcggc gcctacacgg agttcttctg gaccgtgaac gcccgtgccc
     3721 tgatgaactt cgtctccctg cgtgcggcag agacggccca gcgcgagatc cgtcgctacg
     3781 cgaacgccgt cgaacagttc tttgcggcca agatgccggt cacgcacgcc gcgttcgtcg
     3841 agcgcggccg catcgcgccc taggcaaccg tcggcttcag aacgatctgg gtcggctcgt
     3901 ggttgctcgg tagctggcgt gtgatcaccc agccgttccg gtcgaagtgg ctgaggtcat
     3961 acgccgcgct gaaccgcacg atcgcctcgc cgctcctcac gttgaagctg ccgcagagca
     4021 cgaccggctt gccgcccttc gtcaggtaga ggttgtagta gccgccctcc ggcagctgtc
     4081 gcaggcccgt cgcatggagg agcatcggcc agttgccacc ctcatccgcc ttgccgagcg
     4141 cgagcgtccc tcgcgcattg cgatcgaggc tcgtccccgc gagggtcacg acgcgcacgg
     4201 ggtcgatcga agccgagctc gagcttggcc cggaggactg cccgagcacg aagccgagca
     4261 ccgctgcagt tgcaagcgcg gcagcgagca agagcaaccg ctgctgcctc ggcttgccgg
     4321 ctggccgctg ggactcttcg ggccagggga ccgcgtccat tgccggcgag agctcgggcg
     4381 ggggcccggc ctggacgagc agttcgtgcg cgcgccgcag gcgctgccgc tcctcctcgg
     4441 gcacgtccat gccgaccaga tcgtcgaact ccggcacccg gctcactgca actctccttc
     4501 gaggagatcg gccaggcggc tcagccccgc gcgcgtccgc gtcttcacgg tgccgagtgg
     4561 gatgccgagg aagtcggcga tctcgctctg cgagagctca gcgtaatacg cgagctcgat
     4621 cacgtcgcgc tcgttgccgg acagttcttc gagggcacgg tgcacacgcc acgccacgta
     4681 ggacgtctcc gcgcgttcgg gcggaccggg ctcgaccgtt gccgtgtccg gcacctcggc
     4741 cggcagctca gtgcggttcc tgctccggtc gacgatcgca ttgcgggcaa cggtgtagag
     4801 ccagggcgcg ccggccccgc gctctggccg gtacgtcttc gcagaacgcc agatggccgc
     4861 gaacgtctcc tgcacggcgt cctccgcccg catccggtca ccgaggcggc ggagagcaag
     4921 accgaagaca gggcgggcat agcgctggta gaggagctcg aaggcgtcgg cgtcgcgccg
     4981 agcgacgcgg cggatcaggt cgccgtcggt cagcccgcgc tcgtgcagcg gggtggctcc
     5041 ggtcgtttcc cgactcctgc catggggcat ggcagcaggc aaggcaatac tgatgcccat
     5101 cttctcgggg tttctacggg atttcacgtc tgatggattg cagcagactg ggggctccct
     5161 ccacaaccac cagccatacc gaaagcttaa aaacccctta cggggcccgc acgtctaagg
     5221 gaaagagaga ggaaaacact agggggttcc agaatgcgca ggttcattcc gatcgccgtg
     5281 gcagggatgg cactggtgct ggccgcaccg gcgtcgacgg cgaccaccac cgttcagatc
     5341 aagagcacgg ggttcgttcc ggccaccgtg acgatcaacc agggcgactc cgtcacctgg
     5401 acgaacaccg acacgaagga tcaccaggtg gtttcgaacg caggcgcgtt cgcgtcagcg
     5461 atcctgaagc cgggcaagag ctacacgcac gcctttccga gcggcgggac gtaccgctac
     5521 cacgacggcc tccacccgag cctgaagggc gcggtcaccg ttcgcggagc gccgccgcag
     5581 gtgacgctgg ccacctcggc tcccgtcgtg aagttcggga cgcaggtgac gctcaccggc
     5641 acagtgaaca acaagaagac cgccgagact gtgacgatag ccgcgctgcc tttcggacag
     5701 accacgaagc aggtggtcgc aacgctccag acgacgagtg gcggcgcgtt ctcgttcaac
     5761 gtcacgcctc agctcaacac gacctacgag gcacagtgga agggagccgc gagctcggtc
     5821 ggcgtccagg tccagccgac ggtcaagctg ccgttcgttt cccggtcggg ctatttccac
     5881 ttctacgtga cggccggtca gtcgttcgca gggcgattcg tctacctgca gcgcttcacg
     5941 ctggcgcgca cgtggatcaa catccgcaaa ctgcagctcg gccagcagtc cggccggatc
     6001 atggggatga agttcgtccg ctcggtgatc ccacgcgggc gctggtcgat ccggatctac
     6061 atgcccgcga gcgagatgcc gggcgggtac atcgactcgt ggagcgggac gcagccggtg
     6121 gtgaagcgct gaccagtgtg agagcgcgga ctacttgtcc gcgctctcca acctctgctg
     6181 gacggcgtcc cagttgacga cgttccacca ggcctcgagg tactcgggcc ggcggttctg
     6241 gtacttgagg tagtaggcgt gctcccacac gtcgatgccg aggagcggca cgtcagagtt
     6301 cgagatgggt gagtcctggt tcgccgtgga gtacacggcg agacccgtgc cgtcgtagac
     6361 gagccagctc caccctgagc cgaaccgctt gacgccggtg tcgttcatca gcttcttcag
     6421 ctcatccagg ctgtcgaagg tgtcgttgat cgcgtcggcg agcgagccgc tcggctcccc
     6481 gccgccgttc gggctcatga tctcccagaa gaggctgtgg ttggcgtggc cgccggcgtt
     6541 gttgcgaact gccgcgcgga tgtcgtccgg cacgatctcg atgttggcga gcacgtggtc
     6601 tacgggctgg ttctcccact cggtgccggc cagcgccttg ttggcattgt ccacgtaggc
     6661 ctggtggtgc ttgtcgtggt ggaggtgcat cgtctgctcg tcgatatgcg gctcgagcgc
     6721 ggcatagtcg tacgggagag gcgggacctc aaacggcatc ggtagctccc ttcagtggct
     6781 ttcctaaatc ggtcacgagg gatccttgcg gatctgggct ctgttgggtt gaccggtcat
     6841 ttccgacgga cgtcgggcag agcccgccgc cctgatgcgg caccgcagag cggtgccttg
     6901 gccgaggata tgctccgcgc gccggtctag tcgaggagag ggagagatga cgtcgcttgc
     6961 tttcaagttc gaggagatca ccgcccggga gctcaagcct gagctctacg agctcgacgg
     7021 gatctcgcgc gccagcatcg aggcccatta caagctctac cagggctacg tgaacaaacg
     7081 gaacgagatc ctcgggcggc tcgacggtgt cgataccggg agcgcgaatc aggtgtattc
     7141 cgacgtccgc gcgctcaagg tcgacttcag cttcgcgatc ggcgggatca agaaccacga
     7201 gatctatttc gagcaccttg ggggtggcgg aggtgacccc accggcctga tcggcgacct
     7261 gatcgaacgc gacttcggct cggtgcagaa ctggcgtacc gacctgaagg ccaccgggat
     7321 ggccggccgc ggctgggcct ggacggcgta cgactgggac gagggccgcc tcttcaacta
     7381 cgtcggcgac gcactgaaca cctatccgat ctggaacgcg acgccgctga ttgcgctgga
     7441 cgtctacgag catgcctact tcctcgacta tcagaccgat cgcgcctcgt acatcgacgc
     7501 gttcttcgac aacctcgact ggttgacggt caacgactgg gtctcgaagt accagatcca
     7561 gaagtaactc cagatgagaa ccgtgctcct tgtcgctgcc ggctacgtgc tcggctcgat
     7621 gccgtggggc tactggctgc ccttgctcgt caagcacgac gacgtcagac ggcacggcag
     7681 cggcaacatc ggcggaacga acgtgtggcg cgtctacggc tggcgcctcg gcttgcccgt
     7741 cgtgctgttg gacacgctga aggggttcgc gccggcgctc gtcgcgacgc tgatggtgtc
     7801 gcacctcgcc ggggtcctcg cgggagcggc ggcgatgctc ggccactggc gtccgttgtt
     7861 cctgcgctgg cagcgcggcg gcaaggtggt ggcgacgacc ggtggtgcgt tcctcggcgt
     7921 tgcaccggtc gtcggcgccg tgggcgcggg ggtctggatc gtcgtcttcc tcgccttccg
     7981 ttatgcgtct ctggcttcga tcgtcggggc actctcgtta ccggtggcgg cggtcctatt
     8041 gggcgagccc tggccggtga tcgccttcgc cggcggcgcg gcagccgcgg tcctggtgct
     8101 gcaccgcccc aacatcgccc gcctgcgcgc gggcaccgaa aaccgtttca aattcaggcg
     8161 gcggcgggcg agcgctccgg cctcaccgga ccgcgtcgga acacagagcg ggcgttgaga
     8221 cggccgcacg ggcaaagccc gcacggcctt catgcggcgc cgccgagtcg gcgcctaggc
     8281 taagacgcga gccagtcgcg tagctcggtg tgccgggccg ccggctcgcc gtcgcgcagt
     8341 tcgccgcgta gctcgccgac ggtcccgtag cgctctccca gcacgtcgtg cgaatggatc
     8401 gtctcgaagt tgagctggcg cgcgagcacg aaatcctcgt ccttcaggtc cggatagcgg
     8461 tcgagcgtcg accgcaggga gatccggacc gagtcctcga cgaaacgggg ctggagatgg
     8521 gcgtgctcta cgacgaagag ctcgtccggc cgcttgagca gctcgtagat cggcgagctc
     8581 atcgagttct cgacgatgcc ggcgagctct tccgcgttca gggtctggtc ggttccgatg
     8641 tagagcgttc cgcggccgcg ctggttgtga gtcgcaagcg ggacgagctc caggatccgt
     8701 tcgacgtcgg gctcttcgaa ccccgcttcg agcagtcgct ccgacgcctt gccgcgcacg
     8761 agcccctgtg cgcacgggca ggcgttgatc ccggttgcct cgacgccgac gatgcgacgc
     8821 acgcgcgact cggtcgccgc agcgatcccg acgatcgaga tcatctcctg cgtcagcagg
     8881 ccggtcaccg gagttcgccg ctcgagtggg taccgcgccg cgatccgcac ctcagcgcgg
     8941 cccgctctct ggcgaccgac gatgtgtcgc gcgatgtgct ccgccagctc ctcgacgagg
     9001 aaggcctcgc cgatcacgac ctcgtcgatc gcctcctcga agagctccgg gaagcgggac
     9061 atgtgcacgc ccttctggct cgggtcgagg tcgacgaagc agtcgatttc cgcggagatg
     9121 agcttctccg cgccgcccca gctgaggcgg atcgccttcg cgacgccctg gaccccggcc
     9181 ctcgacaggc cgagccgcac ctctggtgtc gaggcctgga gatcgtccag gagctcgcga
     9241 gactccgtca tgtacgacag gttagtgtca gaccgctcag tggtggattc agacgccagt
     9301 ttcccgcggc tcgtctcact cgccgctcac gacctgcgca cgccgctggc gacgattcac
     9361 ggcttcgcgc agacgctcgt gcgcatgggc gatctcggcg agcccaagca gcgctacctg
     9421 gagatgatcg acagtgcagc gcttcaactt gccgagctgc tcgatgagct cggtgtcgcc
     9481 gcacggatcg agggcggacg ctacgagccg gcgctgcagc ccgccgacac gctcgagctc
     9541 gcgcgtggag cggccgagca gctcggggag gaacgggtct tggtgtcagg acagggtggg
     9601 gaggtgcggg tcgacggcga ggcgacgcag cgcggaatct cggcgctcgc gcaggcggcg
     9661 ctacgtcacg gcgggctcga gcagatcgac ttacgcgtcg acagcgcaac gctggcgatc
     9721 tcaccggtca cccccgcctc aggcccggtc ctgctcgggc aggagctgcg agatctcggc
     9781 gcagcggtcg ccgtgatcgt catcgacgca atcggcggct cggtcgcgct ggacggcgac
     9841 acgctgacgg tcaagcttcc ggagtgaacc gagccgcgca ggtcagtctc cggcgctggt
     9901 tgcgccgcca gttgcaacag cccacgcccg ggcgcgagca tctcgaggcg gcggtcgagc
     9961 acaacgatcc tgccgaggtg cggcgcctgc ttgccggtgt gccctttacc gatgcccagc
    10021 gaaggcacgt cgagggcctc ctcgacgcgt gggaggaagc gcgctaatct tcagcgccgt
    10081 tctcgagtag caactgctcg atgcgcgcgt cgcggttctg ccgcgccgcg tcgatggctc
    10141 ggaacccgcc gccctgctcg aggttcggat ccgcgccgtg ctcgagcgcc agcttcacca
    10201 gctcgtagcg tgtcgcctca tccgtcccct gggcggccgc gccggcatgg atcgccgctg
    10261 tctgcacgtg ctcgttcgtc gacagcgcat tgacgtcggc gcctcggtcg agcaaaagcc
    10321 gcgccgcctc gacgtgaccg aagaaacacg cgagcccaag gggatgaaag ccgtcgtcgc
    10381 cgaactcgtt cgcgcgggag ggatcctcgt cgagcagctc ccggagacgg tcggtcctgc
    10441 cgaaagccgc tgcctcgaag acatccagct tcggatcggc agcgagcaac tcatcgacgc
    10501 gcacgcgatc gccctgatag agagcctgga gcaagtcgga catcagcgca gcaatcgtga
    10561 cagacgccga tccttcagga tgcgcccacc cgtctgacac tcggggcagt agtagatcgt
    10621 gtgctcttcg aaatcgacct gcgcgatcgg ggtgccgcac acgtagcagg gctcgccgag
    10681 tttcttgtgg acgcgatagg tgcggttgtc gttcgctccg gcctcgcgga gctccaaccc
    10741 gcgttcgagc tcggtgtcga tcgcgttcgc cagctgctcg acctgctccg ttgtgacgtc
    10801 gcgggtgagc tcgtacggcg acagctgcgc gtgatgcagg atctcgttcg cccaggcgcg
    10861 cccgatcccg gcgatcacgc gctggtcgcg cagcagcgaa tgcagccgcc gggattcgga
    10921 ggcacagatc tcggccaagc gctcggcgcc gaggccgagc gcctcaggcc cgagatggga
    10981 gagctcctgc tccgcctctt caggcgtgag cagccagact cccgcacgct tcttcgcccc
    11041 ggcctccgtc agaacgagcc gcgcgccgcc ttcgaagacg agctggaaag ccggtgtctt
    11101 cggccccgcc tcgcccgtct tgaggtactt caaccgcccg gccgtcatca ggtgcacgag
    11161 caccaccagt tcgccgtcgg tcgtcggaaa gagcaagcgc ttcgcgcgcc gatctgcccc
    11221 cgcaagctgc ctcccttcga gcgccgagag cggcggatcg aacgtcttca gcgtggcgat
    11281 gtgcgccggt cccgccttcg cgatcgggaa cgcggaaacc gggtctgtga gcgcgcggcg
    11341 ccacgcctcc atctccggca gttcaggcac tcgctttggc ctttaggctc caacgcatcg
    11401 acccaatcag ggtatccgtg cgtcgagggg gtgtgaccga ggcggttcac cgcgtccacg
    11461 ccgtcgcggt tcaggacgga gccgtgatcg cagaggcagg cgacacccag ctgctcgcgt
    11521 tcatgcgctc ctcttccaag ccgctgcagg cgattccgct cgcacgtgcc cgcgccgacc
    11581 tcgaggagcg cgacctcgcg attgcgtcgg cgtcccacct cgccgacgac gcacagctca
    11641 cggccgtgcg cgccctgctt gcgaaggctc cggcgaccga agacgagctc gagtgcgggc
    11701 ccgcggggca gccgccgtcg cggctcaagc acaactgttc cgggaagcac gccggcatgc
    11761 tcgcgctctg ccgcgccaaa ggttggcgca gcgagggtta ccggctcgag ggacaccgcg
    11821 cgcagcgcga gatgctcgcc gttcacgccg aggcgtcgca gattctcgag gacgagatcc
    11881 gcaccgcgat agacgggtgc ggcgtcctca ccttcggatt gccgctggaa cgaatggcca
    11941 atgcgttcgc acgtctgccg gaactggaag ggggcgacaa ggtcgcggcg ggaatgcgtg
    12001 cgtatcccga tctgattcga ggaccgatcg cgagcgacac gcgcctgatg aaggcgctgc
    12061 caggatggat cgcgaaaggc ggtgccgaag ggctcttgtg tgcggcgggc agcggcatcg
    12121 gcgttgcgct gaaggcggag gacggaagcg ggcgcgggct cgggcccgcg gcggctgcgt
    12181 ttttccggca actcgacctc gatctcggcg acctcgccgt tgtcccgctc gagaacagcc
    12241 gcggcgagct agttggagag atcactatct aaaaaaaatc gctctttggt tttccgaaac
    12301 cctgtaatat ccgcccggcg agagggtgag ccggggggac gcgggtatcc ggctcttttg
    12361 cgttcgtaga tcgaatcccc gtccaccgac gcgaaaacag ccgggaggta ggcagccgtt
    12421 gctcaccgtc gaaatctccc ttcctgaggt cgaggagctg caaaagctcg tccaggaagg
    12481 tctcgagaag ggccatctca actacgacga gattgcagca gggctcgacg acgtcgagct
    12541 cacgaaggag cagatcgaag acttctacac gtacctggtc gaccacggcg tcgagctgct
    12601 ggagggtgag acgcataagc atccgccgca cgagctcgcc gcacctgaag aagagaaggg
    12661 cccgaagctc gacctgagcg tcgagccgtc gctcgactcg ctgcgcctct acctgcgcga
    12721 gatcggcaag gtctcgctgc tcacagccga ccaggaggtc tacctcgcaa agcgcatcga
    12781 gcggggcgac atgtcggcga agacgcagat gatcgaggcg aacctgaggc tcgtcgtctc
    12841 gatcgcgaag tcgtatctag gtcgtggtct cagcttcctg gatctgatcc aggaaggctc
    12901 actcggcctg atccgcgcgg tcgagaagtt cgactatcgc aagggctaca agttctcgac
    12961 gtacgcgacc tggtggatcc gccaagccgt tacgcgtgcg atcgcggaca aggcgcgtac
    13021 gatccgcatc cctgtgcaca tggtcgagaa gctgaacaag gtcgttcaca tcgagcgcca
    13081 gctcgtccag cgcctcggac gcgagccgcg tcccgacgag atcgccgaag agctcgagat
    13141 gaccacggac gaggtgcgtg agatcctgcg catgtcgcag ctgccgattt cgctcgagaa
    13201 gccgatcggc gaggaggagg actcggagct aggcgacttc gtccaggacg agtcggccga
    13261 gtcgccgttc gacaccgcca cgctgtcgct acgccgcgag gacgtcgagc acgcgctgtc
    13321 cgcactgccc gagcgtgaac gtcaggtgat cgagcttcgc ttcggcttgt ccggggcgca
    13381 gccctgcacg ctcgaggaag tcgggcgggc attcggcgtc acgcgagagc gcatccggca
    13441 gatcgagaac aacacgctca agaagctcga atcgcttccg gaggcccaag ggctgaagga
    13501 ctgcgtttag cggaagcgga cgagctcggc gcgcacgctg acggcgcggt cgccgaggta
    13561 gccgggccgc gccgagctga agtgcaggct ccaggggccg ccggcgggga cgcggaagct
    13621 gagttggatg cgctgccgcg gatgcacgtg gatggtgcgc ttgattcccc ggcctgtgag
    13681 gacgatgggc gtgacccggg cgtgcgccgg caacgcgagc acgagctcga gcgtcccgcc
    13741 ggtcttcgtc cacacggtga tcgcaccctg tggcgcgagc cacttgtcca cgaagcgtcc
    13801 ggcggcgagc agacgtaggc gcggcgttcc caccggacgg tagaggtcga agatcagctc
    13861 gtgccggacg cgctcgacac cgctgagctg gacggtggac gcatacgtct ggacgagcag
    13921 gggccgacga tcgggccgac cgtccacgag cagcgtcccg tcgcctgcga tcttcagctg
    13981 cttggcgtcg aactgatcga tcggtgcact gccgagcaga aggaggcgtt tgacggacgt
    14041 gttccagaag agctgttgcc acgattgctc tttgcgcgag ccgggcggcg ccactaggtc
    14101 gacgctcccg agccgcgcat ggtcgaccca gcggatgtcg tgagggagcg tgctacggag
    14161 gcttcggctc gtccgggcat caaacgagga tgcgcccgca gacattgcgc agcacgcgac
    14221 gatcgcggcc acgacggcga cggccgcggg cagcttgcgg aaggcgacga gcgcgacgag
    14281 cactgacagc accgcagcga cgagggcgaa caccaacgag ccgttggcca ccgacgtcag
    14341 accttcgaac cgcaacactg cccacagtgt gggtgagtcg tccttgttgt gagccgccgc
    14401 atatccggag agcgggacgc gcgccgcgag cagaagcaac ccggcggaaa gcaggcccac
    14461 ggggactcga ccgggcagcc cgcgcttgac gtacagaccg aacgcgactg cgagcagcgg
    14521 gacgagagtg aagagatagc gctcctggaa gcgctgcgaa tcggtgtctg cgatctgggc
    14581 cgcttcgagc agcaggccac agaccagcag caagctcgtc actgcgaacg cgatctcggc
    14641 gcgcctccgc ggccgaaaga gcacgacggc gagccccgcc agcgcgccgg ggacgatcgc
    14701 ccagccggcg gagtacacga gcagcatcgc ctccagaccg acccagtgca ggatcgagac
    14761 agggtgcagt gcgtggttgc cgtgcgcata cacgccgaac acgcggtcgg agccgagcgt
    14821 cgccagcaac agtgcaagtg gaacgatcaa caggacgagc gcgagccaga ggcggcgcac
    14881 ggcctgcacg acgtggaaac ggtcggccac gagctcggcc gtgagcactg cgagcggaac
    14941 gatcacgtac tggatccgcg ccaggatcgc aagcgtgctg aacacgctga aggcgagctg
    15001 tgcgcgcaga gtcggttgcg acacgacgca aacgcctgcg tagaccgccg agaggacgag
    15061 cggataggca agcgggtccg cgagcatcgt cgaggagtag acgccgtcgg gtacggcaac
    15121 ggtcagcgcg gcaacggcga tgccaagcca gcgcgcgagc ccgagccgcc ggcagagcag
    15181 gtaggccgga accgcggcga gcgagaagag cacggcgtga atgccctggg tgagacggaa
    15241 tgccgtcaaa ggatcgtccg tcacgagcca gaccggcgcc gtcacgatcg ggtcgagcag
    15301 tgcggggaag tgcacgccgg cgccgcggat gagcgggcgc ccgtgctcgg cgaggctgcg
    15361 cgcgagcgag gggtagatgt actcgtccgg caggtagtac ggagtcagcc gcgcgacggc
    15421 cgcaaagagg cgccctccga agctggccag gacgatgccg gcgacccaga cccaggccgg
    15481 cacggccgcc acgcgcgcga aggcccgggg tgccgagact tccggtccgg ggcgagaagc
    15541 agcgacgtcg gtggccacga aatccgtatc ggcaagccct cggcatccct tgaacgaggg
    15601 atgcccgccg aaccgcgcgt acagcagaac cacgaaggcc ggaggctaag accaggcgga
    15661 gccgggctta gccggaggcg aactgatgcg ggcgcggacg cttcagcgcc cgtgcccgca
    15721 cggaagggaa caaccgccca gcgccaacac cattggagtg ggcgggggcc gccccccccc
//
LOCUS       DASVEU010000064         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_824323,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000064 DASVEU010000000
VERSION     DASVEU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824323"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF225_02600"
     CDS             <1..339
                     /locus_tag="VF225_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7144173.1"
                     /translation="ARIIDRLGFLNDAVPAIRHHHERWDGTGYPDKLRGEEIPLGARI
                     IHVADALDSMLTTRIYRAARPAAEALQELRRAAGTQFCPRCVTALERILPLESLDSES
                     DRGRLSAVAS"
     gene            391..1083
                     /locus_tag="VF225_02605"
     CDS             391..1083
                     /locus_tag="VF225_02605"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144174.1"
                     /translation="MARKPPFRAVAKIDPDALASFQAGIRKRYSNAQILEELRASAER
                     LGRSPTMREFAADPDTAVHPQTVIEHFGSWNAAKRKAGLVPRRFATREELVGLLRELG
                     EQLGRVPTAKDLDARRGSMPSKSLYWHTFGSLAGALREAGFDVPIGEERLERAVEQGV
                     ELARKLERLPKFADWAAARRKDDGLLTEWQVYRMFDARRGAWSTFQFLIKERLDEEGV
                     RVGSDGQLGLGR"
     gene            1135..2841
                     /locus_tag="VF225_02610"
     CDS             1135..2841
                     /locus_tag="VF225_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX7144175.1"
                     /translation="MSGEESRGLAEEQAALRRVATLVARGVPPTEIFAAVAEEIGRLI
                     SIDGTRILRYEADGTATVIAGWSESVEVPPELEVGARLALEGASVSTLVFRTGRPARI
                     DNYADAAGPLSQALQGSGVRSATGAPIIVEGSLWGVMTAGSTKPEPLPSGTELRLAQF
                     TELLATAIANAESRADLARLAEEQAALRRVATLVAGGAPPEEVFATVTEEVGRLLDTH
                     LAGMARYDSDDTVTVVATWAAEDEHGGAHPLVPGPWPLEGGDLASTISRTGRPVRIDD
                     YRGVPGRIAAFVRDELGIVSSVASPIVVEGRLWGTLFIHSRQTQPLPLDTEARLTGFT
                     ELVATAIANAESRGQLMASRARIVAAADETRRRIERDLHDGIQQRLVSLGLELRAAQA
                     TVPAQLDELEGALARAAEGLTSVLGELREISHGLHPAILSEGGLEPALRALRRRSAIP
                     VELDLHPKRRLPGRVEVAAYYVVSEALTNAAKHARASVVNVELDTHDAILQLAIRDDG
                     IGGADPDQGSGLVGLSDRIEALGGTLQVTSPAGNGTTLLIEVPLEGQQTNAPQPAHAT
                     NS"
     gene            complement(2850..3248)
                     /locus_tag="VF225_02615"
     CDS             complement(2850..3248)
                     /locus_tag="VF225_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144176.1"
                     /translation="MLDRLLRRIWLLGVPVGLVGAAAVIAYGRPWHDAPAPPREVSVE
                     QATLRPGTIVLDVVNNSEETARVAQVILNDAFVDFDQSRLRLEPGSAERITVSYPWIA
                     GEAYDVQLMTATGATVAYEIEEAAAGMQQA"
     gene            complement(3252..4301)
                     /locus_tag="VF225_02620"
     CDS             complement(3252..4301)
                     /locus_tag="VF225_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7144177.1"
                     /translation="MRRSFTRRRALGAAAAVAGSGVAVRLSHLSSTAADAAAPPHQHH
                     PLVPGSSHAGFAHGSTTVDPQVNGFDPSVIMRDFDWGKTRRLAGGRMLREWDLVAYDK
                     QIEIVPGVKFSAWTYNGRVPGPTLRAREGDLLRIHFANAGTHPHTIHFHGIHPAVMDG
                     TPGIGEEAGGGLIDVGEKFTYEFDAQPFGLHLYHCHASPLAGHIAKGLYGMFIVDPPQ
                     GRPHADELVMMQNAFDPNFNEDNEVYAVNTVAFHFMYRPVRVKREDLVRIYLVNIVEL
                     DPINSFHVHGNFFHRFPTGTSLSPSEYTDTVIQGQGQRDILELRFPYTGKYMFHAHKS
                     EFAALGWMGLFEVVE"
     gene            complement(4378..>5180)
                     /locus_tag="VF225_02625"
     CDS             complement(4378..>5180)
                     /locus_tag="VF225_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144178.1"
                     /translation="DTLCGIAGYSLALDSTVERTVAARALLTGIAERGADAVGYAYRS
                     PGAPIAVHKQRSGATALLERISVPEEATKLLVHVRDFTKGHPSLAANNHPIRHGSVVG
                     IHNGIIKNDDELFAQHGIGRAEPGMTVDSEIIFALAERSRGRTADALQQLYGSMATAW
                     LDEGRAELLLARGLGRPLWLGHTKKELFFASTRVALELVESYVGIKLRKSELPEGTIV
                     AVESGKIVTRESFEPDRSFEEEPLPAVRAPDEGRSCLEHLARLHAAAA"
BASE COUNT          823 a         1726 c         1820 g          811 t
ORIGIN      
        1 gcgcgcatca tcgaccgcct cggcttcctc aacgacgccg tgccggccat ccggcaccat
       61 cacgagcgct gggacgggac ggggtatccg gacaagctgc gcggcgagga gatcccgctc
      121 ggcgcgcgga tcatccacgt cgccgacgct ctcgactcga tgctgacgac gcgcatctac
      181 cgggccgctc ggcccgccgc cgaagcgctg caggagctca gacgggcggc aggcacccag
      241 ttctgcccac gctgcgtcac cgctctggag cggatcctgc cgctcgagag cctcgacagc
      301 gagagcgacc gcgggcgtct ctccgcagtc gcctcctagc cgctcaagcc ttaacttgcg
      361 ttctttccag aacgctctat ccttattggc atggcccgca aaccgccctt cagggcggtc
      421 gcgaagatcg atcccgacgc gctcgcctcg ttccaggccg gaatccgcaa gcggtactcg
      481 aacgctcaga tcctcgagga gctgcgcgcg tccgccgagc ggctcggccg ctcgccgacg
      541 atgcgagagt tcgcggccga ccccgacacg gccgtgcatc cccagacggt gatcgagcac
      601 ttcggcagct ggaacgccgc gaagcgcaag gcaggcctcg ttccgcgtcg tttcgccacg
      661 cgcgaggagc tggtcggcct gctgcgcgag ctgggggagc agctcggccg cgtgccgacc
      721 gcgaaggacc tcgatgccag acgcgggtcg atgccctcga agtcccttta ctggcacacc
      781 ttcggttccc tcgcaggggc actgcgtgaa gcaggcttcg atgtgccgat cggcgaggag
      841 cggctcgagc gcgcggtaga gcagggagtc gagctcgcac gcaagctcga acgcctgccg
      901 aagttcgccg actgggcggc cgcgcgccgg aaggacgacg ggcttctgac cgagtggcag
      961 gtctaccgga tgttcgacgc acgccggggg gcctggtcga ccttccagtt cctgatcaag
     1021 gagcggctgg atgaagaggg cgtgcgggtc ggctcagacg gacagctggg cctaggacgg
     1081 tgaccaggca ggggtacgct taccagtcga tcgtcagggg atgggggagc gccggtgagc
     1141 ggtgaggagt cgcgtgggct ggccgaggag caggcggcgt tgcgccgggt cgcgacgctg
     1201 gtggcgcgag gggtgccgcc gactgagatc tttgccgccg ttgccgagga gatagggagg
     1261 ctcatctcga tcgacgggac gagaatcctt cgctacgagg ccgacggcac cgcaaccgtg
     1321 atcgcggggt ggagcgagtc cgtcgaggtt cctcctgagc tcgaggttgg tgcgcgcctc
     1381 gcgctggagg gggcgagtgt ctcgacgctg gtgtttcgga cgggacggcc ggcccggatc
     1441 gacaactacg cggatgccgc cggcccgttg tcacaggcgc tgcagggatc aggagttcgg
     1501 tccgctaccg gcgctcccat catcgtcgaa ggaagccttt ggggcgtcat gacagccggg
     1561 tcgacgaaac ccgagcctct gccgtcggga acggagttac gcctcgcgca gttcacggag
     1621 ctgttggcga cggcgatcgc caatgccgag agccgagccg atctcgcccg cctggccgag
     1681 gagcaggcag cgttgcggcg ggtcgcgacg ctggtggcgg gtggagcgcc gccggaggag
     1741 gtgttcgcga cggtcaccga ggaggtcggg cggctgctcg acacgcacct cgccgggatg
     1801 gcccgttacg acagcgatga cacggtgacc gtcgtcgcta cctgggccgc cgaagacgag
     1861 cacggcggcg cacacccatt ggttccgggc ccctggccgc tcgaaggcgg cgatctggca
     1921 tcgacgatct ccaggacggg ccgacccgtc cggatagacg actaccgcgg cgtccccggt
     1981 cgcatcgcag cgttcgttcg agacgagctc gggatcgtct cctcggtcgc cagcccgatc
     2041 gtcgtcgagg gtcgcttgtg gggcaccctc ttcatccact cgaggcagac ccagccgctc
     2101 cccctggata ccgaagcgcg tctcacgggc ttcacggagc tcgtggccac ggcgatcgcg
     2161 aacgcggaga gccgcggcca gctgatggcc tcgcgggcgc ggatcgtggc cgctgcggat
     2221 gagacgcgga ggcggatcga gcgtgacctg cacgacggca tccagcagcg gctcgtctcg
     2281 ctcgggctgg agctgcgcgc cgcacaggcg accgtgcccg cgcagctcga cgagctcgag
     2341 ggcgcgctgg cgcgggcggc cgaggggctg acgagtgtgc tgggcgagct gcgggagatc
     2401 tcccacggcc ttcaccccgc gatcctctcc gagggcggcc tggagccggc gctgagagcg
     2461 ctgcgccggc gctcggcgat cccggtcgag ctcgacctgc accccaagcg acggctgccg
     2521 gggcgagtcg aggtcgcggc ctactacgtc gtctcggaag cgttgaccaa cgccgccaag
     2581 cacgcacgcg catccgtcgt caatgtcgag ctcgacaccc acgacgcgat cctgcagctg
     2641 gcgatccgcg acgacgggat cggcggagcc gaccccgacc agggctccgg actggtcggg
     2701 ctcagcgacc gaatcgaagc gctcggcggc accctccagg tcaccagccc cgccggcaac
     2761 ggcaccaccc tgctgatcga agtccccctc gaaggccagc agaccaacgc gccgcaaccg
     2821 gcgcacgcga ccaactcctg aaaccgcttc tacgcctgct gcatgccggc cgccgcctct
     2881 tcgatctcgt acgcgaccgt cgctcccgtg gccgtcatca gctggacgtc gtaggcctcg
     2941 ccggcgatcc agggatagga gaccgtgatc cgctccgcgc tccccggttc gagccggagc
     3001 cggctctggt cgaagtcgac gaacgcgtca ttgaggatga cctgcgccac ccgcgcggtc
     3061 tcctccgaat tgttgacgac gtcgagaacg atcgttccgg gccgcaaggt cgcctgctcg
     3121 acgctgacct cacgaggcgg ggccggagcg tcgtgccacg ggcgaccgta ggcgatgacg
     3181 gcagccgccc cgacgagtcc gaccggtact cccagcagcc agatccgtcg tagcaagcga
     3241 tcgagcatca gtcactccac gacttcgaag aggcccatcc agccgagggc tgcgaactcg
     3301 ctcttgtgcg cgtggaacat gtatttcccc gtatacggaa agcgcagctc gaggatgtcg
     3361 cgctggccct gtccctgaat gaccgtgtcg gtgtactcgc tcggcgagag cgacgtgccg
     3421 gtcggaaacc ggtggaagaa gtttccgtga acgtgaaacg agttgatcgg atcgagctcg
     3481 acgatgttga cgaggtagat ccggacgagg tcttcgcgct tgacgcggac gggccggtac
     3541 atgaagtgga aggccacggt gttgaccgcg tagacctcgt tgtcctcgtt gaagttggga
     3601 tcgaacgcgt tctgcatcat cacgagctcg tcggcgtggg ggcgaccctg cggcgggtcg
     3661 acgatgaaca tgccgtaaag gcccttcgcg atgtgtccgg ccagcggcga cgcgtgacag
     3721 tgatagaggt gcaacccgaa cggctgcgcg tcgaactcgt acgtgaactt ctcgccgacg
     3781 tcgatgagcc cgccgccggc ctcctcgccg atccccggcg tcccgtccat cacggcaggg
     3841 tggatgccgt ggaagtgaat tgtgtgtgga tgcgtgccgg cgttggcgaa gtggattcgc
     3901 aacaggtcgc cttcgcgcgc ccgcagcgtc ggccccggga cgcgaccgtt gtacgtccac
     3961 gccgagaact tcacgccggg gacgatctcg atctgcttgt cgtacgcgac gaggtcccac
     4021 tcgcgcagca ttcgcccgcc ggcgaggcgc cgcgtcttgc cccagtcgaa gtcgcgcatg
     4081 atcacgctgg gatcgaaacc gttcacctgg ggatcgacgg tcgtgctccc gtgcgcaaag
     4141 cccgcgtgcg aggagcccgg cacgagcggg tggtgctggt gcggcggagc ggctgcgtcg
     4201 gcggccgtcg aggagaggtg agacagcctg acggccactc ccgacccggc aacggctgcc
     4261 gcagccccga gcgcgcggcg cctcgtaaag gacctgcgca cgatcacctg accgtgccac
     4321 ggtcccctaa gaggaacttg agatgcgcgt tagctgcgcg ttaggagttc ctaagagtca
     4381 ggcagctgcg gcgtgcagtc gcgcgagatg ctcgaggcag gagcggccct cgtccggggc
     4441 gcggacggcc gggagtggct cttcctcgaa ggagcgatcc ggctcgaacg attcgcgcgt
     4501 tacgatcttg ccgctctcga cggcgacgat cgtgccctcg gggagctcgc tcttgcgcag
     4561 cttgatgccg acgtagctct cgacgagctc gagtgcgacc cgggtcgacg cgaagaagag
     4621 ctcctttttc gtgtgcccga gccacagcgg ccgacccaga ccgcgggcca gaagcagctc
     4681 agcgcggccc tcgtcgagcc aggccgtcgc catcgagccg tagagctgtt gcagggcgtc
     4741 ggcagtccgt ccccgtgaac gttcggcgag cgcgaagatg atctcggagt cgaccgtcat
     4801 ccccggctcg gcgcggccga tgccgtgctg cgcgaacagc tcgtcgtcgt tcttgatgat
     4861 cccgttgtgg attccgacga cagagccgtg tcggatcgga tggttgttgg cggcgaggct
     4921 cgggtggccc ttggtgaagt cgcgcacgtg gacgagcagc ttcgtggcct cctccggcac
     4981 gctgatccgt tcgagcaagg ccgtcgctcc ggagcgctgc ttgtgcaccg cgatcggagc
     5041 gccgggactg cggtaggcat agcccacggc gtcggcgcct ctttccgcga tcccggtcag
     5101 gagggcacgc gccgcgactg tccgctcgac ggtgctgtcg agggcgaggc tatatccggc
     5161 gattccgcac aaagtgtctg
//
LOCUS       DASVEU010000065         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1019193,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000065 DASVEU010000000
VERSION     DASVEU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019193"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1043
                     /locus_tag="VF225_02630"
     CDS             195..1043
                     /locus_tag="VF225_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX7144179.1"
                     /translation="MVYDESSNADETEKLSSATEGPEQEQNFGELQFYVPQDLLDVSF
                     PVSVRGYDRRAVGAYIERVNRVIAELKVRSSPPAAVRHALEQAEEKAQGLLRAAREAG
                     EEITTSAQREAEQNTASAKAEAAELLVNTSADADRIKAEADQLIADARMEADAAVAKA
                     QSEADELRAEAKAEAQSTLARAQAEADERLQRLKEELAALREEAETRRGEIQADTEAV
                     WKERHELLEDVRRMASGLVDLANAAAARSHGREPAGSDEESLEERRDDEFREQAAEST
                     AAEPDS"
     gene            1088..>2667
                     /gene="dnaE"
                     /locus_tag="VF225_02635"
     CDS             1088..>2667
                     /gene="dnaE"
                     /locus_tag="VF225_02635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7144180.1"
                     /translation="MPEAPFVHLHVHSEYSILDGACRIPALAARAAEFEMPAVGLTDH
                     GSLAGAVELYREAGKVGVKPILGCEIYVCDDRRAQTKGNAHLTLLAEDNAGYSNLIKL
                     ASAGYLEGYYYKPRVDWELLQSHSKGLIALSGCLSGRVCKALEEARPADAAAELDRLV
                     QIFGQDSTYVEMQNAHLEPQQRINPLLAKMAEGTGLGLVATGDVHYLKHEDARAHEAL
                     LCIQSGDSLKNPQRWKFDTDHFYFKSPEEMALDFPGYDHALRRTLEVAERCNVTMELG
                     GILLPKFPTPDERDSFDYLVELTEKGLQKRYDNSTDELSDRLKFELKTIREMGFADYF
                     QIVWDFIAFAKRNGIQVGPGRGSAAGSLVAYCLDITDIDPIKYDLLFERFLNPGRKSM
                     PDMDIDFAVEGRDRVINYVAEKYGRDRVAQIITFSTMAARAAVRDAGRVLEIPYGVVD
                     KIAKAIPEGPGQTLDACLKPGQELKTQYDADPVAREVVDLARPLEGLVRADSIHAAGV
                     VIGDKPLTEYVPLQQKGS"
BASE COUNT          499 a          877 c          897 g          394 t
ORIGIN      
        1 ctcacggaac agatccgggc tctcgatcga tcccgcctcg gtcggcgcgt cgccacactg
       61 gcgcaggccg agctccgtgc ggtcgacgat gccttgaagg tcgtgctcgg gcttttctga
      121 gccgagctga ggccagtaga gggccgccgg caacgtcgcc ggcggcccct ttcactctgg
      181 ggcatactcg ggacgtggtc tatgacgaat cgtccaacgc ggacgagacc gagaagctct
      241 cgtccgccac cgagggcccg gagcaggagc agaacttcgg cgagcttcag ttttacgtgc
      301 ctcaggacct cctcgatgtc tccttcccgg tctcggtccg tggctacgac cgccgggcgg
      361 tcggtgccta catcgaacgt gtcaatcgtg tgatcgcgga gctcaaggtg aggagttcgc
      421 cgcctgcggc ggtcaggcat gcgcttgaac aggccgagga gaaggcgcag gggctgcttc
      481 gggcggcgcg ggaggccggg gaggagatca cgaccagcgc gcaacgcgag gcggaacaga
      541 acacagccag cgcaaaagcg gaagcggcgg agctcctcgt caacaccagt gccgacgcgg
      601 accgcatcaa ggccgaggca gaccaactca tcgccgacgc gaggatggag gcggacgctg
      661 ccgtggccaa ggcgcagtcg gaggccgatg aattgcgcgc ggaggcgaag gccgaagcgc
      721 agagcaccct cgcacgcgct caggcggagg cggacgagcg cctgcaacgg ctaaaggaag
      781 agcttgctgc gctgcgggag gaggcggaga cgcgacgcgg cgagattcag gctgacacgg
      841 aggctgtctg gaaggaacgt cacgagctgc tggaagacgt gcgcaggatg gcgagcgggc
      901 tcgttgatct ggccaatgcg gcggctgcgc gcagtcacgg ccgcgagcct gccgggtcgg
      961 acgaagagtc gttggaggaa cgccgggacg acgagtttcg cgagcaggca gcggagagca
     1021 cagccgccga gccggactcc tgactcaacc ctgcgtcagc ggaccgcctc actaggatgg
     1081 gcttcccgtg cctgaagcgc cgttcgtcca ccttcacgtc cattccgagt actccatcct
     1141 cgatggggcg tgccggatcc cagccctcgc ggcccgcgcc gccgagttcg agatgcctgc
     1201 cgttgggctc accgaccacg gctcgcttgc cggggccgtc gagctctacc gcgaggccgg
     1261 gaaggtcgga gtcaagccga ttctcggctg cgagatctac gtctgcgacg accgccgcgc
     1321 ccagaccaag gggaacgcgc acctcaccct gctcgccgag gacaacgccg gctactccaa
     1381 cctgatcaag ctcgcctccg cgggctacct cgagggctac tactacaagc cgcgcgtcga
     1441 ctgggagctg cttcagtccc actccaaagg gctgatcgcg ctctccggct gcctttcggg
     1501 acgcgtttgc aaggcgctcg aggaggcccg gcccgccgac gcggccgccg agctcgaccg
     1561 gctcgtccag atcttcggcc aggactcgac ctacgtcgag atgcagaacg cacacctcga
     1621 gccgcagcaa cgaattaacc cgctacttgc gaagatggcc gaaggtacag ggcttggact
     1681 ggtcgcgacc ggcgacgtcc actacctgaa gcacgaggac gcgcgcgcgc acgaggcgct
     1741 gctctgcatc cagtcgggcg actcgctcaa gaatccgcag cgctggaagt tcgacaccga
     1801 ccacttctac ttcaagtcac cggaggagat ggcgctcgac ttccccggct acgaccacgc
     1861 gctccgtcgc acgctcgagg tggccgagcg ctgcaacgtc acgatggagc ttggcgggat
     1921 cctgctgccg aagttcccga cgcccgacga gcgcgacagc ttcgactacc tcgtcgagtt
     1981 gaccgagaaa ggcctgcaaa agcgctacga caactcgacc gacgagctca gcgaccggct
     2041 caagttcgag ctcaagacga tccgcgagat gggcttcgcc gactacttcc agatcgtgtg
     2101 ggacttcatc gcgttcgcga agcgcaacgg catccaggtc gggccggggc gcggatcggc
     2161 ggcgggaagt ctcgtcgcct actgcctcga catcaccgac atcgatccga tcaagtacga
     2221 cctgctcttc gagcgcttcc tcaacccggg tcgcaagtcg atgccggaca tggacatcga
     2281 cttcgccgtc gagggacgcg accgcgtcat caactacgtc gccgagaagt acgggcgcga
     2341 ccgcgtcgcg cagatcatca cgttttccac gatggccgca cgtgcggcgg tgcgcgacgc
     2401 cggacgcgtg ctcgagattc cctacggcgt cgtcgacaag attgcgaagg ccattcccga
     2461 agggccgggg cagacgttgg acgcatgcct gaagccgggg caggagttga agacgcagta
     2521 cgacgccgat cccgtcgctc gggaagtggt cgatctcgcg cgaccgctag aagggctcgt
     2581 gcgagcggac tcgatccacg ccgccggcgt cgtgatcggg gacaagccgc tgacggagta
     2641 cgtcccgctg cagcagaagg gctccga
//
LOCUS       DASVEU010000066         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1408966,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000066 DASVEU010000000
VERSION     DASVEU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408966"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2145
                     /locus_tag="VF225_02640"
     CDS             18..>2145
                     /locus_tag="VF225_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7144181.1"
                     /translation="MPAAKSRAAIAELADEQAALRRVATLVAQDVAPAEIFAAVSAEV
                     DRVFGLDPATFDVAVVGRFEPGPELEIVGISKSVDVGPLGSRWPPDDLFAPTHVLRTG
                     RSARIRADDVVSAGGEVAELLQHSGYLSQVASPIVVDGRLWGAMLVTTRSDLPPDTEE
                     RLERFTELVATALANAESRAALSQLADEQAALRRVATMVARAPASEQLFSDIAREVAS
                     VLDVPGVIVTRYEADGTAVTFGEAFGPDLGGAERFLGVGTSMPPDPGSLAAQVLDTQG
                     PARVDDFSTLPGTVGELARAARLGSGCAGPIVVNGTLWGKMCVFSRAGTVLPGGTEDR
                     LHDFIELVATAIANYEARADRAASEARAGELADEQAALRRVATLVAEGAAPTEVFDAV
                     IAEVGQLLGAAQVGLARYENEHEISVLAIRGQSPEVLRAGVRLPLDGDSVNARILRTG
                     RSARLNFAEDGNGSIAEVLRRDNVNATVGAPIVVDGAVWGMVGASWRGHDQPPADAEE
                     RLAQFAELLDTAIANADSRDQLMTSRARVIAAADESRRRIERDLHDGAQQRLITLTAA
                     LRRADAKLPPGADELRADLTRVAEGLTTAVDELRELSRGIHPSILTEGGLSPALKALG
                     RRSPVRVKLDMGFEQRLPDHVEAAAYYTVSEALTNASKHANATRVWVSLRVDHEMLRL
                     SIRDDGVGGADSNQGSGLTGLKDRIEA"
BASE COUNT          355 a          730 c          740 g          320 t
ORIGIN      
        1 gtctgaggaa cgagccgatg ccggcagcca agtcacgcgc ggcgatcgcc gagctcgctg
       61 acgagcaggc ggcactgcgc cgcgtcgcga cgctcgtcgc gcaggacgtc gcgccggcag
      121 agatcttcgc ggcggtcagc gcggaggtcg atcgcgtctt cggcctagat ccggcgacgt
      181 tcgacgttgc cgtcgtcggc cggttcgaac ccggcccgga actcgagatt gtcggcatct
      241 cgaagagcgt cgacgtaggc ccgctcggat cacgctggcc tccggacgac ctcttcgcac
      301 cgacccacgt tctacgcacg ggtcgctcgg cccgcattcg agccgatgac gtcgtgtcgg
      361 ccggcggcga agtcgccgag ctcctccaac atagtggcta cctgtcgcag gtcgccagtc
      421 cgatcgtcgt cgacggccgt ctctggggag caatgttggt caccacgagg agcgaccttc
      481 cgcccgacac agaggagcgg ctcgaacggt tcacggagct cgtcgcgacg gcgctcgcga
      541 acgccgagtc gcgcgccgcg ctcagccagc tcgctgacga gcaggcggcg ctgcggaggg
      601 tggccacgat ggtggcgcgc gcgccggcgt cagaacagct gttctccgac atcgctcgag
      661 aggttgcatc tgtcttggat gtgccgggcg tgatcgtcac gcgctatgag gccgacggaa
      721 cggcagtcac gttcggcgaa gcatttggcc ccgatctcgg gggcgcggag cgattcctcg
      781 gcgtcggcac atcgatgcca ccagatcctg ggagcctcgc tgcgcaggtc ctcgacacgc
      841 aaggcccggc gcgagtcgat gatttctcga ccctaccggg gacagtcggg gagcttgcgc
      901 gtgcggcacg gctcggttcc ggctgcgcgg gtccaatcgt ggtcaacgga accctttggg
      961 gcaagatgtg cgtcttctcg cgtgcaggaa cggtacttcc cgggggaacg gaggatcgcc
     1021 tccacgactt catagagctc gttgccacgg cgatcgcgaa ctacgaggcg cgcgccgacc
     1081 gggcagcgtc ggaggcgcgc gccggcgagc tcgccgacga gcaggcggcg ctgcgacggg
     1141 tcgccacgct cgtcgccgag ggagccgcgc ccaccgaggt attcgacgcc gtcatcgccg
     1201 aggtgggcca gctgctcgga gcagcccagg tcggcttggc ccgctacgag aatgagcacg
     1261 aaatctcggt ccttgcgatt cgcggccaga gccctgaggt gctccgcgcc ggagtgcgtc
     1321 tgccgcttga tggcgacagc gtgaatgccc gcatcctgcg cacgggacgc tcggctcgac
     1381 tcaacttcgc ggaggacggg aacggcagca ttgcggaggt actccggcgc gacaacgtca
     1441 acgccacggt cggggcgccg atcgtcgtcg acggcgcggt gtggggcatg gtcggcgcaa
     1501 gctggagagg gcatgaccaa ccgcccgccg acgcggagga gcgcttggcc cagttcgcag
     1561 aactgctcga cacggcgatc gcgaacgcgg acagccgcga tcagctgatg acatcgcgcg
     1621 cccgcgtgat cgccgcggcc gacgaaagcc gacggcgcat cgagcgcgac ctgcacgacg
     1681 gagcacaaca gcgactcatc acactgacgg ccgcgctccg cagggccgat gcgaagcttc
     1741 ccccgggagc cgacgaacta cgcgccgatc tcacccgcgt tgcggagggt ctgacgacgg
     1801 ctgtcgacga actccgtgag ctctcacgcg gcatccatcc atcgatcctc accgaaggcg
     1861 gtctctcacc ggcgctgaaa gcactcgggc gccgctctcc cgtccgcgtc aagctcgaca
     1921 tgggcttcga gcaacggctc ccggaccatg tcgaggcagc cgcctactac accgtctcgg
     1981 aggcgctcac caacgcgtca aagcacgcga acgccacgcg cgtgtgggtc tccctccgtg
     2041 tcgaccacga gatgctccgt ctttcgattc gcgacgacgg tgtgggcggc gcggactcca
     2101 accaagggtc cggactgacc gggctgaagg atcgaatcga ggcgc
//
LOCUS       DASVEU010000067         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_49291,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000067 DASVEU010000000
VERSION     DASVEU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49291"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VF225_02645"
     CDS             <1..643
                     /locus_tag="VF225_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7144182.1"
                     /translation="ASTDKSQGHRGLTAFVVPADADGVVVDRHLDKMGQRATDTSALS
                     FQDVRVPVVDRLGEEGEGFKIAMKTLDFTRPGTAIGAVGVARAAFEYAAQYSKERVQF
                     GMPIAMNQGINFMIADMATKIEASRLLCWQAAWMIDQGLRATLQSSYAKRFAADTCME
                     VATDAVQVFGGYGYMKEYPVEKLMRDAKLYQIYEGTSQIQRLVIARELLMPKE"
     gene            735..1565
                     /locus_tag="VF225_02650"
     CDS             735..1565
                     /locus_tag="VF225_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7144183.1"
                     /translation="MIEIDLSGRVALVTGGSRGLGRADALTLARAGADVVIADIQVES
                     DSGEEAEGYGPLAQAARAQGFVYSEATAKEISELGRRSAAIKCDVTDRAQVKETVRRV
                     VDEFGSVDILVNNAGTLDHVGQLPDQVPELWERDLRVNLTGSFNCAQAVWPHMKERGW
                     GRIVNMASVAGTLGGFGQASYATTKAGLLGLTRTLALEGARHGITCNAIVPGVIGTEA
                     FNFGNPAMNERMVQRTAMRRAGEPQDVANAIVFLCSDLASYITGVGLNVSGGIELFTF
                     "
     gene            1621..3510
                     /locus_tag="VF225_02655"
     CDS             1621..3510
                     /locus_tag="VF225_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX7144184.1"
                     /translation="MPARRKYSLVRPRPRRRRRKTRAQRRLFWQRTAILAAVAGLAAM
                     VLGLAFAGSPSRLASGVRIAGIDVGGKTPHQARSALERRADALASVPVTFRVGSRTWR
                     LEPRHLGIRVDWGAAVDAVRRQGNGFGPLRGFRRLDLRFFGADVAPPTQVYDAALERK
                     LDEIAAAVNVPHREASIVLRGLRPQIVPSRTGHVLDRHAAAATIVRALASLKREPVGL
                     PTDLDQPKINAGDLRVVQAEVRTALSAHLHLTLGATRWNLRPPRIARILELPADGRRD
                     LRIGGAGASSWFSALAKRVDRSPVDADWAVGKGGIRVIPDQPGYVLDVPRSAKAVLRA
                     ALVTEPALRDADLTVEHVDADRTTADAQAMDIEGLVASYQTFYGGEPNRIHNVQLVSH
                     LVDKHVIAPGETFSFNGATGARTEDKGFLEAPVIINGELKTGLGGGVCQVSTTVFNAA
                     YEAGLPIVSRTNHALYISHYPQGRDATVNYPDTDLKFTNDTGHWLLLRTWVGSSSLTV
                     ALYGTPVHRRVVSETAPLVVTGPPPVKKVPDPSLFVGQTVVEEKGSPSLKTSNERKVY
                     DADGKLLFDTTFYSSYRGEMKIIRIGSKKRPKDQTDTTTTDTTTTTTTTTTKKTTTTT
                     TTPRP"
     gene            complement(3488..3904)
                     /locus_tag="VF225_02660"
     CDS             complement(3488..3904)
                     /locus_tag="VF225_02660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7144185.1"
                     /translation="MEGFRFSTDVTVRFAETDAQGVAHNANYLVWFEVARVAYLAEYA
                     GGYSAIRGQGFESFVLESHVRYLQPARFDDRLRVHTRVGEVRGARFRFDYEITRDGEV
                     IANGWTSHACVDAKTLRPTRIPQNLADAIARAEGSS"
     gene            3923..4663
                     /locus_tag="VF225_02665"
     CDS             3923..4663
                     /locus_tag="VF225_02665"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7144186.1"
                     /translation="MAHTVSDLAALIRERQPCVVLTGAGISTESGIPDFRSPGGIWAQ
                     YDPMEYATITAFRRDPVKVWEFYALRFELLTTAEPNEGHLALAELERRGLVEAVVTQN
                     IDGLHGRAGSEAVVEVHGSIRSASCLECGERVPLEDVIAALEDAPAPPCPRCGAVLKP
                     DVVMFGELLPAAAIDRAVELSQRAGLLLVVGSSLEVHPVAGLPAETVSAGGVVAIVNR
                     RPTPFDHLASIRIDAGAGETLSALAEEV"
     gene            complement(4660..4806)
                     /locus_tag="VF225_02670"
     CDS             complement(4660..4806)
                     /locus_tag="VF225_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144187.1"
                     /translation="MTLRRHKHTAITIHAIRQRAGVPYHVERKVCSNCSRLLEEKTLK
                     RAAA"
     gene            4908..5663
                     /locus_tag="VF225_02675"
     CDS             4908..5663
                     /locus_tag="VF225_02675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7144188.1"
                     /translation="MRALAVIPAVGLTLLGGCGGNGERPDVAADIAAKARVCAPGSIQ
                     RLGSTRLAYAAIVRSQATVSRRPGAHAFASFERLNVNRVPTIFSIRGALVDAGCKPTS
                     YLVQIPKRPNGVTAWVPAAQVEVAKVSTRIVVDLSERRVRLYKGGELVLSSKAAVGAP
                     ATPTPTGRFYVNQRLIPADTGGPFGPGAVGVSAFSNVLTGWTQGGPIAIHGTNAPSSI
                     GKAVSNGCIRVPNAVLTKLFDQALSGTPVLIRN"
BASE COUNT          939 a         1942 c         1966 g          911 t
ORIGIN      
        1 cgcgtccacc gacaagtcgc aggggcatcg cgggctgacc gcgttcgtcg ttccggccga
       61 cgccgacggc gtcgttgtcg accgtcatct cgacaagatg gggcaacgcg ccacggacac
      121 gtcggcgctg tcgttccagg acgtgcgcgt ccccgtcgtg gaccggctcg gcgaggaggg
      181 cgagggcttc aagatcgcaa tgaagacgct cgacttcact cggcccggca ccgcgatcgg
      241 tgcagtggga gttgcgcgcg cggcgttcga gtacgcggct caatactcga aggagcgcgt
      301 gcagttcggc atgcctatcg cgatgaacca gggcatcaac ttcatgatcg ccgacatggc
      361 gacgaagatc gaagcgtcgc ggttgctctg ctggcaggcg gcgtggatga tcgaccaggg
      421 gctacgcgcg accctgcagt cctcgtacgc gaagcgtttc gcggcggaca cctgcatgga
      481 ggtcgcgacc gacgcggtgc aggtcttcgg cggctatggg tacatgaagg agtacccggt
      541 cgagaagctg atgcgcgacg cgaagctgta ccagatctac gagggcacct cccagatcca
      601 gcggctcgtg atcgccagag aattgctcat gccgaaggaa tgagttccgg cgggtcgacc
      661 ggtcagttcg gacggatgcc cggcggagcc gggcatcctg atgacggcgc cgacaagcgg
      721 cacctccggt ccgcgtgatt gagatcgacc tctcgggtcg agtcgcactg gtcacaggtg
      781 gttcgcgcgg cctcggtcgc gccgatgcgt tgacgcttgc gcgcgccggc gccgacgtcg
      841 tgatcgcgga catccaggtc gagtccgatt ccggcgagga ggccgagggc tatgggccgc
      901 tcgcgcaggc cgcccgtgcg cagggcttcg tctattcgga ggcgacggcg aaggagatct
      961 cggagctcgg gcgccgttcg gccgcgatca agtgcgacgt gactgatcgg gcccaggtga
     1021 aagaaacagt gcggcgcgtc gtcgacgagt tcggctcggt ggacattctc gtcaacaacg
     1081 ccggcacgct cgaccatgtc ggccaacttc cggaccaggt gccggagctt tgggagcggg
     1141 acctgcgcgt gaacctgacc ggctcgttca attgcgccca ggccgtctgg ccacacatga
     1201 aggagcgggg atggggacgg atcgtgaaca tggcgtccgt tgcgggaacg ctcggcggtt
     1261 tcggtcaggc gagttacgcc acgacgaagg ccgggctgct agggctgacg cgaacgctgg
     1321 cgctcgaggg agcaagacac gggatcacgt gcaacgcgat cgtgccgggc gtgatcggca
     1381 ccgaggcgtt caatttcggc aatcccgcga tgaacgagcg gatggtgcag cgcacggcga
     1441 tgagacgggc cggagaaccg caggacgtcg cgaatgcgat cgtcttcctc tgctccgacc
     1501 tcgcgtccta catcaccggc gtcggcctca acgtcagcgg cgggatcgag ctcttcacgt
     1561 tttaagcagg ccgttccgaa cagaaagaac ccgccagacc cgtggctaga cttcgcaacc
     1621 gtgcctgctc ggcgcaaata ctccctggtc cggcctcgcc cccggcggcg tcgcaggaag
     1681 acgcgcgccc agcgccgtct gttctggcag cgaaccgcga tcctcgcggc ggttgccggc
     1741 ctcgctgcga tggtgctcgg cctggccttt gcgggctcgc cctcgcggct cgccagcggc
     1801 gtccggatcg caggcatcga tgtgggcggg aagacgccgc atcaggcgcg ctcagccctc
     1861 gagcgccgcg cggacgcgct cgcctcggtc ccggtgacct tccgcgtcgg ctcgcggacg
     1921 tggcggctgg agccacggca cctcgggatc cgcgtggact ggggcgctgc cgtcgacgcc
     1981 gtccgccgcc agggcaacgg cttcggcccg ctgcgcggct tccgcaggct cgatctgcgc
     2041 ttcttcggag cagacgtcgc gccgccgacc caggtctacg atgccgccct ggagaggaag
     2101 ctcgacgaga tcgcggccgc ggtgaacgtc ccgcaccgcg aggcgtcgat cgtgctgcgc
     2161 ggtctcaggc cccagatcgt cccgagccgc accgggcatg tgctcgaccg ccacgccgcc
     2221 gccgccacga tcgtgcgagc gctggcgagc ctcaagcgtg agcccgttgg cctgccgacc
     2281 gacctcgatc agccgaagat caacgccggc gatctgagag tggtacaggc ggaggtgcgt
     2341 accgccctgt cggcccatct ccatctgacg ctaggggcga cgcgctggaa tctccgcccg
     2401 ccgcgcatcg cccggattct cgagctgccg gccgacggaa ggcgcgatct ccggatcggc
     2461 ggcgccggag cgagcagttg gttttcggca ctcgccaagc gcgtcgaccg ctctccggtc
     2521 gacgccgact gggcagtcgg caaaggcggt attcgcgtga tcccggatca gcccggctat
     2581 gtgttggacg tgccgcgcag cgcgaaggcc gtgctgcggg cggcgctcgt aaccgaaccc
     2641 gcgcttcggg acgccgacct caccgtcgag catgtcgacg ccgatcggac gaccgcagat
     2701 gcgcaggcca tggacatcga ggggctcgtc gcgagctacc agacgttcta cggcggcgag
     2761 cccaacagga tccacaacgt gcagctcgtc tcacacctcg tcgacaagca cgtgatcgcg
     2821 cctggcgaga cgttctcctt caacggcgcg accggcgcgc gcaccgagga caagggcttt
     2881 ctggaagcgc cggtgatcat caacggcgag ctgaaaaccg gtctcggggg cggtgtctgc
     2941 caggtttcga cgaccgtgtt caacgccgcg tacgaggccg gattgccgat cgtctcgcgc
     3001 acgaatcacg ccctgtacat cagccactac ccgcagggcc gcgatgcgac ggtcaactac
     3061 ccggacacgg atctcaagtt cacgaacgac accggtcact ggctcttgct gcggacctgg
     3121 gtcggatcgt cctcgctcac cgtcgcgctg tacgggacgc cggtgcaccg ccgggtcgtc
     3181 agcgagacgg ccccgctcgt cgtcaccggc ccaccgcccg tcaagaaggt tccggatccg
     3241 tcgctcttcg tcgggcagac cgtggtcgag gaaaagggat caccgtccct caagacgagc
     3301 aacgagcgca aggtctacga cgcagacggg aagctgctct tcgacacgac gttctactcg
     3361 tcgtatcgcg gcgagatgaa gatcatccga atcggctcta agaagcgccc gaaggatcag
     3421 acggacacga cgacgaccga cacgactacg acgacaacca cgacaaccac caagaagacg
     3481 acgacgacta cgacgacccc tcggccctag cgatcgcgtc cgccaggttc tgcgggattc
     3541 gtgtcggccg gagagtcttt gcatcgacgc aggcgtgcga ggtccagccg ttcgcgatca
     3601 cctcgccgtc ccgcgtgatc tcgtaatcga agcgaaagcg cgcgccgcga acctccccaa
     3661 cacgtgtgtg gacgcggagc ctgtcgtcga accgggcagg ctgcagatag cggacgtgcg
     3721 actcgagcac gaaggattcg aacccctgtc cgcggatggc cgagtaaccg cccgcatact
     3781 cggcgagata ggcgacccga gccacctcga accagacgag gtagttggcg ttgtgcgcga
     3841 ctccctgagc gtcggtctct gcaaagcgga ccgtcacgtc ggtgctgaag cggaagccct
     3901 ccacgcgcgt cactctacgg ctgtggcaca caccgtttcc gacctggcgg cgctgatccg
     3961 ggagcgccaa ccctgcgtcg tcctcaccgg cgccggcatc tcgacggaga gcggcatccc
     4021 cgacttccgt tcgccgggcg gaatctgggc gcagtacgac ccgatggaat acgcgacgat
     4081 caccgccttc cggcgggatc ccgtgaaggt ctgggagttc tacgcattgc ggttcgagct
     4141 gctcacgacg gccgagccca acgagggcca cctcgcactt gccgagctcg agcgccgcgg
     4201 gctggtcgag gcggtcgtga cacagaacat tgacgggctg cacgggcgcg ccggctccga
     4261 ggcggtggtg gaggtgcacg gttcgatccg ctcggccagc tgcctcgagt gcggcgagcg
     4321 ggttccgctc gaggacgtca tcgccgcgct ggaggacgcg ccggcgccgc cgtgcccccg
     4381 ctgcggagcg gtgctgaagc cggacgtcgt catgttcggg gagctgctgc cggcggccgc
     4441 gatcgaccgc gcggtcgagc tctcccagcg ggccggcctc ctgctcgtcg tcggctcgtc
     4501 gctcgaggtc cacccagtgg cggggctgcc cgccgagaca gtctctgcgg gtggggtggt
     4561 ggcgatcgtc aaccgcaggc cgacgccgtt cgaccacctc gcatcgatcc ggatcgatgc
     4621 aggtgccggc gagacgctct cggccctggc cgaagaggtc taggctgcgg cgcgcttgag
     4681 ggtcttctct tcgaggagcc ggctgcagtt cgagcagacc ttgcgctcca cgtgatacgg
     4741 gactccggcg cgctgccgga ttgcatgaat ggtgatcgcg gtgtgcttgt gcctgcgaag
     4801 cgtcattact tcctctcaga gtaatcagac gttcggtcgt caaggaatgt tgtcggcaga
     4861 tgttcaccga accttcacac cctttttgca cctataccct ctgccgcgtg cgcgccctcg
     4921 ccgtcatccc ggcggtcggc ctcaccctct tggggggctg cggcggaaac ggggagcgcc
     4981 ccgacgtcgc ggcggacatc gctgccaagg cgcgtgtgtg cgccccgggc tcgatccaga
     5041 ggctgggctc gacacggctg gcgtacgccg cgatcgtgcg ttcgcaggcc acggtctccc
     5101 gccggccggg agcacacgcc tttgccagtt tcgagaggct gaacgtcaat cgcgtcccca
     5161 cgatcttctc gatccgcggc gcgctggtcg atgccggctg caagccgacg tcgtacctcg
     5221 tccagattcc gaagcgcccc aacggggtga ccgcatgggt cccggccgct caggtggagg
     5281 tggccaaggt ctcgacccgg atcgtcgtcg acctgtcgga gagacgggtc aggctctaca
     5341 agggcggcga gctggtgttg agctcgaagg ccgccgtcgg agccccggcg acgccgactc
     5401 cgaccggccg cttctacgtc aaccagcgcc tcattcccgc ggacacgggc ggcccgttcg
     5461 gccccggtgc ggtcggtgtc tcagcgttct cgaacgtcct gaccggctgg acgcagggcg
     5521 gcccgattgc gatccacggc accaacgccc cgtcgtcgat cggcaaggcg gtctcgaacg
     5581 gttgcatccg cgttccgaac gcggtgctga cgaagctgtt cgaccaggcg ctcagcggca
     5641 cgccggttct gatccggaac taggggagac gcaaccgcgc ccagacgccg gcgtggtccg
     5701 agggccagag gccgtcgagc cggtcgccgg gagcctcgcc gacgatcccc gcacccac
//
LOCUS       DASVEU010000068         9491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1605407,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000068 DASVEU010000000
VERSION     DASVEU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605407"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VF225_02680"
     CDS             complement(<1..441)
                     /locus_tag="VF225_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144189.1"
                     /translation="MRALEASVRHRYGAGRRGCAGLVMRRRPIRQAAGCLKASGSHQE
                     DDVRITTAFNRRRLRCPACGIDLEAVAWARPGAGHTRDFEDTGAWLAQQMAKTQITRL
                     LRIGWSTVGQIVTRVVADQLDERRLSGLVAIGCDEISYRRGQRYL"
     gene            504..905
                     /locus_tag="VF225_02685"
     CDS             504..905
                     /locus_tag="VF225_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144190.1"
                     /translation="MSDYSVAQTLHTAMTADSLSYIPMPKDELRDQLEIGRIENPRAA
                     RIVEEFEKAGLLVHPAPTKNQTFTRVFRLDTELGTFVHELLGAQGEADDEQAFARAAR
                     RLEAASPAEVFKRMREVFEEEGWVRFRQATE"
     gene            complement(1229..2156)
                     /locus_tag="VF225_02690"
                     /pseudo
     CDS             complement(1229..2156)
                     /locus_tag="VF225_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904945.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(2390..3277)
                     /locus_tag="VF225_02695"
     CDS             complement(2390..3277)
                     /locus_tag="VF225_02695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7144191.1"
                     /translation="MATDNCVLLGDNLDVLPTFADGSFQMIYVDPPFNTGKQQVRRTL
                     STEADLEGDRTGFGGRRYATKLLRESSYLDEFDDYLAFLEPRLRQARRVLAMTGTFYF
                     HIDYREGHYCKLLLDEIFGRENFLNEIIWAYDYGARSKSRRPAKHDTILVYVKDPAAY
                     YFDAEEVDREPYMAPGLVTPEKVARGKLPTDVWWHTIVPTNGREKTGYPSQKPEGIVR
                     RMVLASTRPGDRCLDFFGGSGTLGAVAAKTGRRFVLIDSNPEAVRVATERLDRLDVET
                     PASFVHAADEHSAINGALF"
     gene            3427..3789
                     /locus_tag="VF225_02700"
     CDS             3427..3789
                     /locus_tag="VF225_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144192.1"
                     /translation="MTDDPRLVAGTINARGRVTFNQVTQVQATDLPEEIVLVAVLPNE
                     GLYVWRLPHEDVDVEAHEQHAGYSRQVGPIDPNHPPPWLGAPAIVGSGDIDPALWKQI
                     TSARTNTLKKLVGRALGL"
     gene            3980..4198
                     /locus_tag="VF225_02705"
     CDS             3980..4198
                     /locus_tag="VF225_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144193.1"
                     /translation="MTLSRRWPRCVGGGVDVHVRRARRRGRALQPELGARARQRDRGA
                     AIGQPDQRARDELRLLQRQPRHLQTQPA"
     gene            4361..5233
                     /locus_tag="VF225_02710"
     CDS             4361..5233
                     /locus_tag="VF225_02710"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144194.1"
                     /translation="MRRQLAALDDYAAKARKAYDLPQSDGVEASGKAWQELFGAKFCK
                     PPAAKALPEASFSFRAADGEQDIEADLGFPISPGTNYQVVVRGRVSKKSDRLFGYDLA
                     KHGNRVRKHRSLAFEITRCTVPKPYDTYWKVRNDGEEATRLNVLRGQIELGNAHRSEI
                     TAYRGNHYVEIYIVKDRRCVAIDRQRVRSTSADVPLMNDARGPRAVRSCVASHSSTSL
                     GGQQGPRVISLSFAFHFAVAGRFISVTSHSQPCTMHYADSLVMPTRVRSSCVKDRENS
                     PVATMGISHLVGGR"
     gene            complement(6153..6764)
                     /locus_tag="VF225_02715"
     CDS             complement(6153..6764)
                     /locus_tag="VF225_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144195.1"
                     /translation="MALFSERGYDAVTIADIAEATDVAKQTVVNHFAAKEDLLFAWHR
                     PVEADLIDMIDQLPLGSSLPEFLKDELPRLFAHLSEVPEAPDGKDPRTAVDGRLAGVG
                     TIIQNSPGLQDALRLRGTRYQENLAAILSDRVPAHLGAVAARAIAAFILSTVAAMIDE
                     ATRLRGAGQSSEQIAATLTDTVGQALDILHTGIGNIGGSPAFS"
     gene            7565..8929
                     /locus_tag="VF225_02720"
     CDS             7565..8929
                     /locus_tag="VF225_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7144196.1"
                     /translation="MSVGDPFADACRLRHLLARREVSSREFVGSCLERIERLDGDLRA
                     FRVVMKARALSAADAADRALREGDERPLLGVPVAVKDVVDVAGETTSLGTGAVTRRAQ
                     ADGALVRRLEAAGAVVVGKTNLSELQIWALTASSTWGVTRNPWNAERSPGGSSGGSAV
                     AVAAGMVAVAHGVDGGGSIRLPAAWCGLVGFKPAPDAVVLSRQSWHGLAVHGPLARSV
                     RDAALLAAVLAGDRRPDASNVVAAARHLPPGCLRIAVSVSPPVPAPVDPPVRDAVLRT
                     ARVLEALGHHVEHWEVFGGLRDAQRVGLAVLARFLDGACAHAALLERPELLEPRTRAV
                     LRAGRLVPQRALRAAQRAQAREQRRLATTLAGVDALLMPVSARIAPHADAWDGLGATR
                     AFGRQSRLIPFTPPWNLVGWPALALPAGRDPNGVPIGVQLVAAPGREPALIALAAQLE
                     SAQP"
     gene            complement(9096..9362)
                     /locus_tag="VF225_02725"
                     /pseudo
     CDS             complement(9096..9362)
                     /locus_tag="VF225_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
BASE COUNT         1516 a         3213 c         3189 g         1573 t
ORIGIN      
        1 caggtagcgc tggccgcgac ggtaggagat ctcatcgcag ccgatcgcga cgaggccgct
       61 gaggcgccgc tcatcgagct ggtcggcgac gacccgggtg acgatctgcc cgaccgtgct
      121 ccagccaatg cgcagcagcc gagtgatctg cgtcttggcc atctgctgcg ccagccacgc
      181 gcctgtgtct tcgaagtcgc gggtgtggcc ggcgcccggc ctggcccacg cgaccgcctc
      241 gagatcgatc ccgcacgctg ggcagcgcag ccggcgccga ttgaatgctg tggtgatgcg
      301 cacgtcgtcc tcctggtgtg agccgcttgc cttcagacag cccgcagcct gccggatagg
      361 acgacggcgc atcaccaacc ccgcgcaacc cctccggccg gcgccgtagc ggtgccgcac
      421 gctcgcctca agcgcgcgca cacgcacagc ccccgtggct cgtcaactca gggcgctcgg
      481 cgcctccatc ccatggagaa atcatgtctg actacagcgt cgcgcagacg ctccacaccg
      541 caatgaccgc cgactcgctg agctacatac cgatgccgaa ggatgagctt cgagaccagc
      601 ttgagatcgg acgcatcgaa aacccgcgag ccgctcgaat cgtcgaggaa ttcgagaagg
      661 ccggactgct cgtacacccc gcgcccacga agaaccagac attcacgcgc gtcttccgtt
      721 tggacacaga gctcgggacg ttcgttcacg agcttctcgg tgcccaggga gaggccgacg
      781 acgagcaggc atttgcgcgc gctgcgcgcc ggctcgaggc agcgtcaccc gccgaggtat
      841 tcaaacgcat gcgcgaagtg ttcgaggagg aaggctgggt tcgattccgc caagccaccg
      901 aatagcggcc gtgccgcagc ctcacgccgc gtgccgccgg ccacacgacc ccaccgaccc
      961 cccgacgctg tgcaggcgcg tcccagcgcg acggtcggca gcccgggcgc cgctggcgcg
     1021 aacccgcttg gctaagcccg ggtgctcatc gtgccctctc ccctttcgct ggcgatggta
     1081 gataacgtcg ttacgggcta tccatctaac cggagctggg cgcgttggcg cttctccacg
     1141 gccgatctgc gggcgcccgc tcgaggctct ctggtcgtcc gcgggtcgcg ggcgcgacgc
     1201 ggtgggtggt gctgggccgc cggttgagga tcaggtcgcg gtagcgaacg ggatcatggg
     1261 cgacggcgag ttcgagcacg cggtagaaga ccatgccgcg gctgcgtgag cgtcggcggt
     1321 tgaaccgaaa gacgaactcg tcgaggtagc ttgagaggtg cggctcctcg acggcgccct
     1381 ggtgagtgcc aagcagccag cgttgcgcga gcgaggcgat gcgatgcacg ccgggcagca
     1441 ggtcggaggc cttcgcggcg ggcgcgacga ctcgctcatg gacgtacagg tccttggtcg
     1501 ccggtcggta gggctgccag ccgtcgctga tgacggtcgc gcccggctgg acgtgatcga
     1561 tcagaaacgc gcgcagtgac gcgctggacg catccagcat ccacgccatg cgacaccgcc
     1621 cgaggccttg ggctcaagcg ctcaaccgcg atcccgacca gtgccttctt gcccttcgca
     1681 cggccgcccg ccaggcccgg ctcttcgccg ccgatgtacg tctcgtcgac ctcgacacgg
     1741 ccctgcagca gctcgcggcc ggggcgcacg agcaccgagc gcaggcgatg cagcatcgcc
     1801 catgccgtct ggtacgagcc gatcccgagc gcgcgctgaa gaccgagcgc cgagatcccg
     1861 tccttctgcg tggcgaacag ccagcacgcc atgaaccaca ccgtcagcgg tgtccgtgtg
     1921 cggtcgaaga tcgtgcccgc agtcggcgaa gtccgcgtct tgcagccggc acacatccac
     1981 cgcccgtcgc cgagccgcca gccaccgtcg tgggcacagg ccgtgcacac gaagccgtcg
     2041 ggccagcgaa gccactccag gtagtccaga cagtccgcat cggtcccgaa ccacgacgag
     2101 aactcgccca gcgaccgcgg gtaatgcaga ccggcggtcg gtgtcgggcg ggacaggaaa
     2161 gcagcctact ccggttagat ggatagcccg tacgtcgttt atcacttggt cctcggcacc
     2221 tcggtgttac cgctgcccgc tgtcccatat caatgtgaga ggtcgtttgc gacgacgaca
     2281 tcgtccggcg ccatcgatgg cgatggcagg accccgcgcg ctcaacgctt cgcggtgccc
     2341 ttacttactc tgccgcgaaa cgacggaact cggtgcgatt agaaggggct tagaacagcg
     2401 caccgttgat cgcggaatgt tcgtccgctg cgtggacgaa cgatgccggc gtctcgacgt
     2461 cgagcctatc cagacgctcc gtcgctacgc ggacggcttc aggattggag tcgatcaata
     2521 cgaatcggcg tcctgtcttc gccgcgacgg cgccaagtgt cccgctgccg ccgaagaagt
     2581 ctaggcatcg atcgcccgga cgtgttgacg ccagcaccat ccggcggacg atgccctccg
     2641 gcttctggct cggatacccg gtcttctcac gcccgttcgt aggcacgatg gtgtgccacc
     2701 agacatccgt cggaagcttt ccgcgcgcta ccttctccgg cgtcacgagg ccgggagcca
     2761 tatacggctc ccggtcgact tcttccgcat cgaagtagta ggccgcagga tccttgacgt
     2821 agacgaggat cgtgtcgtgc ttggcgggcc ggcggctctt cgaccgggca ccatagtcgt
     2881 acgcccagat gatctcgttc aggaagttct cgcgaccgaa gatctcgtcg aggagcagct
     2941 tgcagtagtg cccctcgcgg tagtcgatgt ggaagtagaa cgttcctgtc atagcgagca
     3001 cgcgtcgagc ctgccgcaac ctcggttcaa ggaaagcgag atagtcgtcg aactcgtcaa
     3061 gatacgacga ctcgcgcagc aactttgtgg cgtatcggcg gccgccgaac ccggttcgat
     3121 cgccttccag atcggcctcc gtggacagcg tgcgtcgaac ttgttgcttg cccgtgttga
     3181 acggagggtc cacgtagatc atctggaatg agccgtcagc gaacgtcggt aggacatcga
     3241 ggttgtcgcc caggaggacg cagttatcag tcgccatacc gcgtacgcta cgcaacgcgt
     3301 cggaaggacg gacgcgcgag gtaccgtgct gcgtaggcgg gcagaacggg gtcacggctc
     3361 ctcgtgctgc atctgatcgg cgctacccgc ccttgcatgc tcgggtggac gggtaggctc
     3421 ggctcaatga ccgatgaccc gcggctcgta gccggcacga tcaacgcgcg ggggcgcgtc
     3481 accttcaacc aagtgacgca agtccaggct acggacctcc ccgaggagat cgtgcttgtc
     3541 gcagtcctgc ccaatgaggg cctttatgta tggcgccttc cgcatgagga cgttgatgtg
     3601 gaggcgcatg agcagcatgc aggctactca cggcaggtag ggccgattga tccgaaccac
     3661 cctccgccgt ggctcggtgc gccggcgatc gtcggttcgg gtgatatcga ccccgcactc
     3721 tggaagcaga tcacctcagc acgaacgaac accttgaaga agctcgtcgg gagggcactg
     3781 ggcctctgag gttaggcgtc gcgtgtctga cgtgcttggc gcgagaagcg cagctctacg
     3841 cgatgcgccc cggacgtcgc cttgggaact gcctttccca atgtgaccgc accgctaccg
     3901 tccagcaccg acgggggaga ggacccggag gcggccggga aactgcacac aaaccacgcc
     3961 gtagacgtct cgaacggcga tgacgctctc gcgacgctgg ccacggtgcg tcggcggcgg
     4021 ggtcgacgtg cacgtacgcc gcgcgcgtcg ccgcggccgt gcgctgcagc ccgagctcgg
     4081 tgcacgcgcc cgacagcgcg atcgcggcgc ggcgatcggg cagcccgatc agcgcgctcg
     4141 cgacgagctg cgcctgctgc agcgtcagcc acggcacctc cagacacagc cggcctagcc
     4201 agcgcgcgcc ggcacgcgca aagcgcggat cgtcgacgac gagcagcacg agcgccaagg
     4261 cgtcctcgag gccgtgtgct cgagcgggga ctcagcgcaa gacgctccac ccatggctct
     4321 acataacgca cgttatcgag cattgattcg gcctgagtcg atgcggcgcc agctcgcggc
     4381 gctggacgac tacgccgcga aggcacggaa ggcatacgac ctgcctcaga gcgacggcgt
     4441 cgaggcgagc gggaaggcgt ggcaggagct gtttggagcc aagttctgca agccgcccgc
     4501 ggcgaaggcg ctcccggaag cctcattctc cttccgcgcc gcggacggag agcaggacat
     4561 cgaggccgac ctcggctttc cgatctcacc cggcaccaac taccaggtcg tcgtccgggg
     4621 gcgcgtcagc aagaagagcg accgcctgtt cggctatgac ctcgccaagc acggcaaccg
     4681 cgtccgcaag caccgctcgc tcgcgttcga gattacgcgg tgcaccgtcc cgaagccgta
     4741 cgacacctac tggaaggtgc gcaacgatgg tgaggaggcc acgcgcctga acgtgctgcg
     4801 cggacagatc gagctcggca acgctcaccg ctccgagatc acggcctacc gcggcaacca
     4861 ctacgtcgag atctacatcg tcaaagaccg gcgctgcgtc gcgatcgacc gccagcgcgt
     4921 ccggtctact agcgctgacg ttccgttgat gaacgacgcg cgcggtccac gggccgtgcg
     4981 ctcgtgcgtc gcctcccact cctcgacctc gctcggcggc cagcaaggtc cgcgtgtcat
     5041 ctcgctgtcg tttgcgtttc acttcgctgt cgcgggccgt ttcatctccg tgacgtctca
     5101 cagccagccc tgcacgatgc actatgcgga ctcgctggtt atgccgactc gtgtgagaag
     5161 ctcctgtgtc aaggatcgcg agaattcccc agttgcgacg atgggaattt cccaccttgt
     5221 gggcggccgg tgagggctgg gtgtagaggc gatggcgttg cggtggcggc gcatgcggta
     5281 gctgtcgccg gtgatcgaga gcacggtggc gtggtgtagg aggcggtcga ggatcgcggc
     5341 ggcgacggtc gtgtcttcga agatcgcgcc ccagtcggcg atgccgcggt tggtcgtcag
     5401 gacgatcgag ccgtgttcgt agcggcggct gatgacctgg aagagatgcg aggcgtcgtg
     5461 gccgggcatc ggcaggtagc ccagctcgtc gatcagccat cttcagggcg acgcgcggaa
     5521 gcccgtcgtt gtgcagcgcg ttctccggct cgagccggcg gcgatagtcg gccgttccgt
     5581 gccggcccgc aacctccgca aggaagatcg cggtctcgac cgcttcgcgc tggcagaaga
     5641 gaaccggctc ctcgcggtcg ctccagtgcg acagcagctt gcgcgtgtac ggggtgacgc
     5701 cgttccaatt gccagcgcgc cacttctcta cctcgcgccg gatgtcgttg atcagcgtgt
     5761 tctgctcacg ccgctcgccc gtcacgtcga agtccatgac gagcgcctcg cccgccggcg
     5821 cccgcccttt cctgctgacg ggaaccggta tgaaaaactc gcttggccgc cgacccgcga
     5881 gcaccttgcc cgttggcccg tcgtcgccca gctcgaaatg cgccgccgga ggctcgtacg
     5941 gcgagttcag gatcggattg ctgaggtcgt gcgtcgtcac gtccgagacc gtagctgcca
     6001 tggcggtctc atcgtgcggt gtcccccacc tacgctgccc gatggcgctc gagtcagcag
     6061 gcgctcgatg tctacgactt ccttgcgaag agccgcttga gctcgtgcca gagaccgagc
     6121 tatgacgtac gccgatccgt ccgctgatcc ggctaagaga atgctgggga tccgccgatg
     6181 tttccgatgc cggtgtgcaa gatgtccagc gcctgaccga ccgtgtcggt cagggtggcg
     6241 gcgatctgct ccgacgactg acctgcgccc cgcagccgtg tcgcctcgtc gatcatggcg
     6301 gcgacagtgc tcaggatgaa cgcagcgatc gcccgagccg caacggctcc cagatgtgcg
     6361 ggcacccggt cagacaggat cgccgccagg ttctcctggt aacgcgtccc gcgcagccgc
     6421 aaggcatcct gcagcccggg actgttctgg atgatggtgc cgacgccggc gagccgacca
     6481 tcgaccgccg tccgcggatc tttgccgtcc ggtgcctccg gcacctccga caggtgggcg
     6541 aacaggcgcg gcagctcgtc cttgaggaac tccggcagcg agctacccag ggggagctgg
     6601 tcgatcatgt cgatcagatc cgcctcgacc ggacggtgcc aggcgaagag caggtcttcc
     6661 ttggccgcga agtggttgac aacggtctgt ttggcgacgt ccgtggcttc ggcaatatcg
     6721 gcgatcgtca cggcgtcgta gccccgctcg ctgaacagcg ccaatgcggt gtgggcgatc
     6781 cggtctctgg tctggcgtcg ccgccgttcg tgccgggact gcgtcaattc ttcctccggc
     6841 tgcggctggt cggtgtcgtc tgtgatggct cccatggaag taccatacca tatttagtat
     6901 cctaccagtt ctggtacgag cctactctct gagaagcgcg ccgacgcctg ggcggcgtgc
     6961 cgaatgctca gcgctggcgc caccggaccg gcaggtcgtg gctcctgcca gccgtccagg
     7021 aagctcgaag aagtactcaa agacggcgac ctactcgtcc tcggggcagt ccccacggcc
     7081 ttggacaaca ggctcgtcac gtcgctgggc gtcctggccg gcttagctga cgtcatcatg
     7141 gctacctcca gtacgacgtc ttgcggctgt atcaccgtga tatcgcgtga tccccgcatc
     7201 ttcaagcctg cctgcaacga tccgaaacga actagagtcg cgggaccacg gccgtgattc
     7261 ctctccggct gcgagcccgg agcactcggc agcgcgtcgg cgatctgctt tgtcagctca
     7321 ggagacacgt ctgcttcctc ctggccgcgc tcgtgcgcgg ccacgtccgc cgtgagacct
     7381 cccgcgtggc cgtggtagcg cgacgacgtc cggcacgcac gcgaacgttc gggcggccag
     7441 cgtccccggc tggcagtcga aggtgatcgc cttcaagcgc gctcggtctg tcattgacgg
     7501 acggtcgtag gtcagcgcag gatgccacga tgccgtgtat gcgcggcctc tgcgaagagc
     7561 tggcgtgagc gtcggcgacc cgttcgccga tgcgtgccgg ctgcgtcact tgctggctcg
     7621 gcgcgaggtg tcgtcgaggg agtttgtggg cagctgcctg gagcgcatcg agcgcctcga
     7681 cggtgacctg cgagcgtttc gagtggtgat gaaggctcgc gcgctgtctg ccgcggacgc
     7741 cgccgatagg gccttgcggg agggcgacga gcgcccgttg ttgggcgtgc cggtggcggt
     7801 caaggacgta gtcgacgtcg cgggggaaac gacgtcgctt gggaccggcg cggtgacgcg
     7861 ccgggcgcag gccgacggag cgctggtgcg gcggctggag gcggccggcg cagttgtggt
     7921 tggaaagacg aacctgtccg agcttcagat ctgggcgttg accgcgtcgt ctacgtgggg
     7981 cgtgacgcgc aacccgtgga atgccgagcg ttcgcctggc ggcagcagcg gaggctcggc
     8041 ggtggccgtg gcggcgggga tggtcgctgt ggcgcacggc gtagacggcg gcggctcgat
     8101 tcgcctgccg gccgcgtggt gcggtctggt cggctttaag cccgcgcccg atgcggttgt
     8161 cctttcgcgg cagtcctggc acggtctggc cgtccacggc ccgctggcgc gctcagtgcg
     8221 agacgcggcg ctcttggccg ccgtgctcgc cggcgatcgc cggcccgacg cgagcaatgt
     8281 tgtggccgcc gcgaggcatt tgccgccggg gtgcctacgg atcgcggtga gcgtctcgcc
     8341 gcccgtgccc gcgcccgtgg acccgcccgt gcgagacgcc gtgctcagga cggcccgtgt
     8401 gctcgaggcg ctcgggcatc acgtcgagca ctgggaggtg tttggcggcc tgcgcgacgc
     8461 gcagcgtgtt gggctggccg tcctcgcgcg ctttctggat ggggcctgcg cacacgccgc
     8521 cctgctcgag cgacccgaac ttctcgaacc ccgcacccgc gccgtcttgc gcgccggacg
     8581 gcttgtgcca cagcgcgcgc tgcgcgccgc gcaacgcgcg caggcgcgcg agcagcgccg
     8641 gctcgcgaca acgctcgccg gcgtggacgc gctcctcatg ccggtcagcg cccgcatcgc
     8701 cccgcacgcc gacgcctggg acggacttgg cgcgacccgg gcgttcgggc gtcagtcgcg
     8761 tctgatcccg ttcacgccgc cgtggaacct cgtcggctgg cccgccctcg cactcccagc
     8821 cggacgggac cccaacggag ttcccatcgg cgtccagctc gttgccgcac ccggacgcga
     8881 gcccgcgctc atcgccctcg ccgcacaact ggagtccgca caaccatgaa cgacgcgccc
     8941 aacagcacca cgacgggccc gcgcgcgaac gctcgtcact ttcttgtgaa ggctccgagc
     9001 gaactgaacg accttgccgg ctcccgcccc ccgcccgacg agtacctcag cctcagtgag
     9061 acggcggtca gcaagaacgt tcgagcattg agcgctcaga ccccgggaag caggccggcg
     9121 aggtgtgcgt cgagcgcggc gcgggcgtcg tcggccgaga gcgccgaggg ggcgcagagc
     9181 agatgccagc ccagcccgtc ggcgagcccg accgcctgcg tcgccgcacg aaccggatcg
     9241 acgccgctgt cggcctggcc gctggccacc gccctgcgca gcgcgtcggc gacggccccg
     9301 gtcagcgcgc cgtagccctc cgcatggagc tcggccagcc gcgggctgtg cgccgaacgg
     9361 gcggtgaacg ctaactactt cccccggaaa aactagtctc ctgaagggtg cgtgcgacgc
     9421 ccggttccgt ccgctttgta tgggataatt gcctgcatat gagcatgttt tatgcgatgc
     9481 agggaactcc a
//
LOCUS       DASVEU010000069         7354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_633865,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000069 DASVEU010000000
VERSION     DASVEU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633865"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF225_02730"
     CDS             <1..796
                     /locus_tag="VF225_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7144197.1"
                     /translation="GNVATGLARLGLRPAIVSRVGPDPHGEFVRDFLSAEGVDVRFLA
                     VDERLMTPPTFCEVWPPDNFPITFYRYPTAPDWQLAPEDFDGAEVATAPFLLATGTGL
                     AQSPSRETTLAALEAHEGTTIFDLDWRPTLWERADEYGELARTAATHADIVIGNEEEV
                     AAAAGVPSALLEVGVRTLVLKRGERGALLFHEGHEVDVPGHPVEVVNGLGAGDAFVAT
                     FVQSLHADLPVEEAVRRGSVAGAIVASQLACSEAMPTLQELETELT"
     gene            793..1638
                     /gene="iolB"
                     /locus_tag="VF225_02735"
     CDS             793..1638
                     /gene="iolB"
                     /locus_tag="VF225_02735"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX7144198.1"
                     /translation="MTELLLRDGEWDVVDPASASWRYLSFRVERLRDGEQVSRQTGGD
                     EVALVPLGGRCAIESGGERWELGERASVFDGMPWALYLPRDTEYTLSALGDVQVAVSG
                     ALADERLEPRLVTPDEVEIEVRGSGNATRQINHIIKPEFPAQRLLVVEVFTPAGNWSS
                     YPPHKHDEDHPPGEVVLEEVYYYRTQSEPPGAFAVQRLYSPQHGTDITETVRDGDIML
                     VPHGYHTTAAAHGYDLYYLNGLAGDRRSMASADDPSLAWIRPAWAKLELDPRVPMVTL
                     EGRRS"
     gene            1728..2546
                     /locus_tag="VF225_02740"
     CDS             1728..2546
                     /locus_tag="VF225_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7144199.1"
                     /translation="MLDAISDAGYEGTELGPPGYLGQRDNLRDRLEDHGLELVGGYIP
                     IRFSEPEHWREDLAGMGETLDLFEAGGGTETKAVLADGGSPERAQFPGRAASDQSIGL
                     DAAGWRRLSEGVARAADLARNRGFEPAFHHHTATFVEAPWEIERVLELTDVGLLLDTG
                     HLAIGGGDPTQALRDWSDRINHLHVKDVRNDVLAGVIADGADMPEAWRRGVFCELGTG
                     DVDLDGFFAELARSRYAGWLVVEQDMVPQSPQDAADAEAAQIRNRAWLKEHASI"
     gene            complement(2543..3523)
                     /locus_tag="VF225_02745"
     CDS             complement(2543..3523)
                     /locus_tag="VF225_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144200.1"
                     /translation="MRRRPRFGTIALALLALGVVGAAVVDTVGKSVSKQHSRAKPHVL
                     PVSEALRSDRDAIAGRLRTDKASGVLYYVDSACGLHALRLPSLAATPAPRGGGCRALV
                     SPASAPPGWSLWPRNTPLAARCDEHRRVIVSAAAGPSLPMIGGCAPAWRPDGSMTYIR
                     RGAIVQFPRTGRAQVLRSRSQLAGALEAQPTLRGVGGWHAIRVAWLSPARFAVLASNG
                     SRAVLAVFSGRRIVALRTRVPSRVTELRGSPRGSYVVLGTAGGVRVYDARRRDLSRVR
                     RFGILVSVAWSSDESWRAVAQEQKVLLVGPHVSVILPLHALDLAWTRNLS"
     gene            complement(3520..4542)
                     /locus_tag="VF225_02750"
     CDS             complement(3520..4542)
                     /locus_tag="VF225_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7144201.1"
                     /translation="MSRATRPLRLGVAGLGGMGTVHAENAQRLSGAELVAVAAAREGR
                     AEEVGRRLGVAHGTYAELFARDDVDAVVLAARSLDHGRLAVDVLRSGKHLFLEKPGAT
                     SLAEHDSLRLESAARPGQVVQVGYMRRYDAGFVEAHRLVRAGEGGTPLVILLTSRDTE
                     WPAGEDPQDTGGFLLDMAAHDYDSACWLFDDEPVAVSVARQALVYPELSDLGDLDNGV
                     VTIRFAGGGIAVTHVSRTSSFGHDIRCEVVGSEGSVFVRGPGDGAANLIDRSDAVRFP
                     LDYRARFADAYVAELTGFVAACTGAGPAGPTLDDDRRAVTTGIAARASAVGGQPLAVG
                     TDWPWL"
     gene            4575..5663
                     /locus_tag="VF225_02755"
     CDS             4575..5663
                     /locus_tag="VF225_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7144202.1"
                     /translation="MRNRFSSLAVSEPQESSAPGVVTRRKTTLADVAEQAGVSTSTAS
                     RALNGHGGLAPATRAAVQDAAAKLNFQPSALARSLRTQQTLTVGFVVPDVSSPFYAAA
                     LKGAQGRLEHAGYRVMLMDASLDVDRELAALETLLSHQVDGLLVSSTGMARSLFDGAV
                     GAAATPTIFFDGVVEGTGNGAVTLDNEGGIALLVDHLVEHGHECIALLAGSQRESAGA
                     ARLHAFHAAMAARGLSPADRYVKVCDWEIADGRRAGLELLADPERATAVVASSAELAL
                     GFMSAAATMGTRVPDDVALVAFDDPYFGDLLDPPLTAVAYDARQIGATAAEMLVEAMR
                     SDTMAVEVRVPVTLVRRRSCGCRPEVSG"
     gene            5702..6775
                     /locus_tag="VF225_02760"
     CDS             5702..6775
                     /locus_tag="VF225_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144203.1"
                     /translation="MTKRYGGQDALQPTDLEIEEGEFFCLLGPSGCGKTTTLNLVGGF
                     IAPTSGEILIRGENVNRLPPYKRAVNTVFQNYALFPHMTVRDNVGFGLKMAGTAKRDA
                     GGRIEEALGLVGLDTFADRLPAQLSGGQQQRVAVARALVNRPAVLLLDEPLGALDLKL
                     RKRLQIELAEIHRDVGTTFVYVTHDQEEAMSMATRIAVMNDGRIEQVGTPHEIYHQPK
                     SAFVADFIGESNFIDVEVGTGGKASLDNGAEVPAPEPRASGRATLMVRPESIQVQRVA
                     DSNGTGLRGRIAQSSFLGSHTRVAVKCDGVSAPVTAALFGNEGAAASDLLPETEVVLI
                     WDPADVVLLEGSNRREEDVRDVN"
     gene            6762..>7354
                     /locus_tag="VF225_02765"
     CDS             6762..>7354
                     /locus_tag="VF225_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7144204.1"
                     /translation="MTSIDERLFDERMSRGRMLRLGAVGAVGVIAAGCGGSSKGSSGA
                     ASATGKSDKAVTLNWLTWSDHYANDQLAAVKQQTNEQGRPQLFSDNADAYLKIKQTGS
                     QFDIVSGDALWVPKYNKDGLTESFDLSSLPVSSQLYSIARTFPFWTDGSNYMGYPFSW
                     STVQIYYNPKFVKTIPDSWHAILDPKYKGKISLENIPT"
BASE COUNT         1241 a         2509 c         2492 g         1112 t
ORIGIN      
        1 gggcaacgtc gcgacagggc tcgcgcggct cgggctgaga ccggcgatcg tctcgcgcgt
       61 cgggcccgat ccgcacggtg agttcgtacg agactttctc tcggcggagg gagtcgacgt
      121 ccgtttcctt gcagtcgacg aacgcctgat gacgccgccg actttttgcg aggtctggcc
      181 gcccgataac tttccgatca ctttctaccg ctatccgacc gcgccggatt ggcagcttgc
      241 ccccgaggac ttcgacggcg cggaggttgc aaccgcgccg ttcctgctcg caaccgggac
      301 cggcctcgcg cagtcaccga gtcgtgagac gacgctcgcc gcactcgaag cgcacgaagg
      361 tacgacgatc ttcgatctcg actggcgccc cacgctttgg gagcgagccg acgaatacgg
      421 cgagctcgct cgaactgcag cgacacacgc agacatagtg attggcaacg aggaggaggt
      481 cgcagcggcc gcgggagttc cgagcgcgtt gctcgaggtc ggagtgcgca cgctcgtgct
      541 caagcgcggc gaacgcggcg cactcctctt tcacgaaggt cacgaagtcg atgtgccggg
      601 ccatccggtc gaggttgtca acgggcttgg cgcgggtgac gcgttcgtcg ctactttcgt
      661 gcagagcttg cacgccgacc ttccggtcga agaggcggtg cgccgcggtt cggtagcggg
      721 cgcgatcgtt gcatcccagc tggcatgctc ggaagcgatg ccgacactgc aagagctcga
      781 gacggagctc acgtgacgga gctcctgctc cgcgacggcg agtgggacgt ggtcgacccc
      841 gcctcggcgt cgtggcggta cctgtccttc cgtgtcgagc ggctgcggga cggcgagcag
      901 gtctcgcggc agacaggagg cgacgaggtc gcactcgtgc cgctcggcgg caggtgcgcg
      961 atcgagagcg gtggcgagcg atgggagctc ggcgagcgcg cgagcgtctt cgacggcatg
     1021 ccctgggccc tgtacctccc gcgcgatacg gagtacacgc tgtccgcctt gggcgacgtc
     1081 caggtggcgg tgtccggagc gctcgccgac gaacggctcg agccacgact ggtcacgccg
     1141 gacgaggtcg agatcgaggt gcgcggctcc ggcaacgcga cgaggcagat caaccacatc
     1201 atcaagcccg agtttcccgc gcaacgtctg ctcgtcgtgg aggtgttcac gccggcaggg
     1261 aactggtcga gctatccgcc gcacaagcac gacgaggatc acccgcccgg agaggtcgtg
     1321 ctcgaggagg tctactacta ccgcacgcag agcgagccgc cgggtgcgtt cgcggtgcag
     1381 cgtctctaca gcccgcagca tggaacggac atcacggaga ccgtgcgcga cggcgacatc
     1441 atgctcgtac cgcacggcta ccacacgacc gcggcagcac acggctacga cctctattac
     1501 ctcaacggtc tcgccgggga tcgccggtcg atggcctccg cagacgatcc atcgctcgcc
     1561 tggatccgtc ctgcctgggc gaagctcgag ctcgacccgc gtgtgccgat ggtgacgctg
     1621 gaaggacgac ggagctgagc agcattcgcg tcgcgaacgc gccctgcagc tacggggcct
     1681 tcgagatcac ggtcggcatc ctgcccgacg ttcccgggcc ggaacgcgtg ctcgatgcga
     1741 tatcggatgc aggctacgag ggaaccgagc tcggcccgcc cggctatctc ggccagcgcg
     1801 acaacctgcg cgaccggctc gaggaccatg gcctcgagct cgtcggcggg tacatcccga
     1861 tccgcttcag cgagcccgag cactggcgcg aggacctggc gggcatgggc gaaaccctgg
     1921 acctgttcga agccggcggc ggcaccgaga cgaaggcggt gttggcggac ggcgggtcgc
     1981 cggagcgcgc gcagttcccc ggccgagccg cttcggatca gtcgatcggg ctcgacgccg
     2041 ccggttggcg gcggctctcc gagggcgttg cccgtgcggc cgatcttgcc cgcaatcgcg
     2101 ggtttgagcc ggcgttccac catcacaccg cgaccttcgt cgaggcaccc tgggagatcg
     2161 agcgtgtgct cgaactgacc gacgtcggct tgttgctcga cacgggccat cttgccatcg
     2221 gtggcggcga ccccacccag gccctccgcg actggagcga ccggatcaac cacctgcatg
     2281 tgaaggacgt acgcaacgac gtcctcgcag gcgtgatcgc cgacggtgcc gacatgccag
     2341 aggcgtggcg acgcggtgtg ttctgcgagc tggggacggg cgacgtcgac ctcgacggct
     2401 tctttgccga gctggcacgt tcgcggtacg cgggctggct ggtggtggag caggacatgg
     2461 tcccgcagtc gccgcaggac gccgccgacg cagaggctgc tcagatccgt aaccgcgcct
     2521 ggctcaaaga gcacgcgtct atctaagaaa ggttccgcgt ccacgccaga tcgagcgcgt
     2581 gcagcgggag aatcacgctg acgtgcggcc cgacgagcaa gaccttttgc tcctgcgcga
     2641 ccgcccgcca gctttcgtcg ctggaccacg cgaccgaaac gagtatcccg aagcgccgaa
     2701 cacgcgagag gtctcgtcgg cgggcgtcgt acacgcgcac gccgccggcc gttcccagca
     2761 cgacgtagct tcctcgtggg ctccctcgca attcggtcac ccgcgacggc acacgcgtcc
     2821 tgagcgcgac gatgcgccgt ccggagaaca cggctagcac ggcccgcgac ccatttgagg
     2881 cgagcaccgc aaaacgggcg ggactgagcc aggcgactcg gatcgcgtgc cagccgccca
     2941 ccccccgtag cgtcggctga gcctcgagcg cgccggcgag ctggctccgg ctccgcagca
     3001 cctgcgcccg gcccgtccgc gggaactgga cgattgcccc gcggcgaatg tacgtcatcg
     3061 agccgtccgg tctccacgcc ggtgcgcagc cgccgatcat cggcaatgac ggccccgcgg
     3121 ctgcgctcac gatgacgcgc cgatgctcgt cacaacgggc cgcgagcggt gtgttgcgag
     3181 gccagagcga ccaacccggc ggcgcggacg caggcgaaac gagggcgcgg cagcctccgc
     3241 cacgcggcgc cggcgtggcc gccagcgatg gcagccgtag tgcgtgcaac ccgcaagccg
     3301 aatcgacgta atacagcaca ccgcttgctt tgtcggtccg caaccgtccg gcgatcgcat
     3361 cgcgatcgct ccggagcgcc tcgctcaccg gcagcacgtg tggcttcgca cgcgagtgct
     3421 gtttggagac cgacttgccg accgtatcga caacggcagc gccgacgaca ccgagcgcga
     3481 gtaacgcgag cgcaatcgtc ccaaaacgcg gtcgccgtct cacagccaag gccagtccgt
     3541 cccgaccgcg agaggctgac cgcccacggc gctggcgcgc gcagcgatac cggttgtgac
     3601 cgcccgccgg tcgtcgtcga gcgtcggccc ggccggacct gcgccggtgc aagccgcgac
     3661 gaatcccgtg agctcggcga cgtaggcgtc cgcgaagcgt gcgcgatagt caagcgggaa
     3721 gcgcacagcg tcggagcggt cgatcaggtt cgccgccccg tcgcccggtc cccgcacgaa
     3781 gacagagccc tcgctcccaa cgacttcaca acggatgtca tgtccgaacg acgacgtccg
     3841 ggacacgtgc gtgacggcga tgccgccgcc cgcgaagcga atcgtcacga cgccgttatc
     3901 gaggtcgccg aggtccgaga gctccgggta gacgagtgcc tggcgtgcaa ccgacaccgc
     3961 gacgggctcg tcgtcgaaga gccagcacgc cgagtcgtag tcgtgcgcag ccatgtccaa
     4021 cagaaacccg cccgtgtcct gtggatcctc gccggcgggc cactcggtgt cgcggctcgt
     4081 gagcaggatg acgagcggcg tcccgccttc gcccgcccga accaggcggt gtgcctccac
     4141 gaagccggcg tcgtagcgcc gcatgtagcc gacctgaacc acctgtccgg gacgagccgc
     4201 gctctcgaga cgaagactgt cgtgctccgc cagcgacgtt gcgcccggct tctcgagaaa
     4261 caggtgctta cccgacctca ggacatccac ggcgaggcgg ccgtggtcca gcgagcgggc
     4321 ggccagtacg accgcgtcga catcgtcccg ggcgaacaac tccgcgtaag tgccatgagc
     4381 gaccccgagc cgcctcccca cctcctcggc gcgcccctcg cgggccgcgg cgaccgccac
     4441 gagctcggct ccggagagcc gctgggcgtt ctcggcatgg acggtgccca ttccgccgag
     4501 accggcgacg ccgagccgaa gaggacgcgt cgcccttgac actgcgagag cgagtatggc
     4561 agcgtcgcaa tcgcttgcgc aatcgatttt ccagcctggc ggtcagcgag ccgcaggaga
     4621 gctctgcgcc tggcgtcgtc acccggcgca agacgacgct cgccgatgtc gccgagcagg
     4681 ccggcgtctc gacctcgacc gcctctcggg ccctgaacgg ccacggcggt ctcgcaccgg
     4741 ctacgcgtgc cgccgtgcag gacgcggccg ccaagctcaa tttccaaccg tccgcgctcg
     4801 cccggtccct ccgaacacag cagaccctca ccgtcgggtt cgtcgtgccc gacgtgtcca
     4861 gcccgttcta tgccgcagca ctgaagggag cgcagggacg gctggagcac gccggttacc
     4921 gcgtgatgct gatggatgca agcctggacg tcgaccggga gcttgcggcg ctggagacct
     4981 tgctctcgca tcaggtcgac ggcctgctcg tctcgagcac ggggatggcg cgctcgctct
     5041 tcgacggcgc tgtgggcgcc gcggcgacgc cgacgatctt cttcgacgga gtggttgaag
     5101 gcaccggcaa cggtgcggtc acgctcgaca acgagggggg gatcgcgctc ctcgtcgacc
     5161 atctcgtcga gcacggccac gaatgcatcg ccttgctcgc cggatcgcag cgcgagagcg
     5221 ccggcgctgc gcgcctgcac gccttccacg ccgcgatggc cgcgcgcggc ctgtcccccg
     5281 cagatcggta cgtgaaggtt tgcgactggg agatcgccga cgggcgccgc gcggggctgg
     5341 agctgctcgc cgaccccgag cgcgcaacgg ccgtggtcgc gtccagcgcg gagctcgcac
     5401 tcggcttcat gtcggccgcg gcgaccatgg gcacccgcgt gcccgacgac gtcgcgctcg
     5461 tcgccttcga cgacccgtat ttcggggatc ttctcgatcc tcccctcacg gcggtggcgt
     5521 acgacgcccg ccagatcggc gccacggcag cggaaatgct cgtcgaggcg atgcgatccg
     5581 acaccatggc ggtcgaggtg cgcgtcccgg tcacgctcgt ccggcgccgt tcgtgcggct
     5641 gccggccgga ggtctccgga tgaacgggag cagcgattca cccgcgatca ccttccgcgg
     5701 cgtgaccaag cgttacggcg gacaggacgc gctccagccg acggacctcg agatcgaaga
     5761 aggtgaattc ttctgtctcc tcgggccgtc ggggtgcggg aagacgacga cgctcaacct
     5821 cgtgggcggc ttcatcgcgc cgacgtccgg cgagatcctg atcaggggcg agaacgtgaa
     5881 ccgccttcct ccgtacaagc gtgcggtgaa cacggtcttt cagaactacg cgctgttccc
     5941 gcacatgacg gtgcgtgaca acgtcggctt cggtctgaag atggccggca ccgctaagcg
     6001 cgatgccggc ggaaggatcg aggaggccct cgggctcgtc ggtctcgaca cgttcgccga
     6061 tcgcctgccg gcgcagcttt cgggcggaca gcagcagcgt gtcgcggtgg cccgtgcgct
     6121 cgtcaaccgg cccgcggtcc tgctgctcga cgagccgctc ggcgccctcg atctgaaact
     6181 ccgcaagcgc cttcagatcg agctggccga gatccatcgc gacgtcggca cgacgttcgt
     6241 gtacgtcacg cacgaccagg aagaggcgat gtcgatggca actcgcatcg ctgtcatgaa
     6301 cgatggacgc atcgagcagg tgggcacgcc gcacgagatc taccaccagc cgaagagcgc
     6361 cttcgtcgcc gacttcatcg gcgagtccaa cttcatcgac gtcgaggtcg ggaccggtgg
     6421 caaggcctcc ctcgacaacg gtgcagaagt ccccgccccg gagccgcgcg caagcggacg
     6481 ggcaacactg atggtgcggc cggaatcgat tcaggtccaa cgtgtcgcgg actcgaacgg
     6541 aaccggcctg cgggggcgaa tcgcccaatc gtcgtttctc ggcagtcaca cccgtgtggc
     6601 ggtgaagtgc gacggtgtca gcgcaccggt cactgccgca ctcttcggca acgagggtgc
     6661 tgccgcgagc gatcttcttc cggaaaccga ggtcgtcctg atctgggacc cggcagacgt
     6721 agtgctactg gaggggtcga accgacgaga ggaggatgta cgtgacgtca attgacgagc
     6781 gcttgttcga cgagcgcatg agccgaggcc gcatgttgcg gctcggggca gtcggcgccg
     6841 tcggagtcat cgcggcagga tgcggaggct cttcgaaagg gtcgagcggg gcagcatctg
     6901 caaccgggaa gagcgacaag gcggtcacgc tgaactggct cacctggtcg gatcactacg
     6961 cgaacgacca gctggcggcg gtaaagcagc agacgaacga gcagggccgg ccgcagctgt
     7021 tcagcgacaa cgcggatgcc tacctgaaga tcaagcagac ggggagccag ttcgacatcg
     7081 tgtccggcga cgcgctctgg gttccgaagt acaacaagga cgggttgacg gagtcgttcg
     7141 acctctcgtc actaccggtc tcatcgcagc tgtattcgat cgcccggacc tttccgttct
     7201 ggacggacgg ttcgaactac atgggctatc cattctcgtg gtcgacggtg cagatctact
     7261 acaacccgaa gttcgtcaag acgatcccgg actcgtggca cgcgatcctc gatccgaagt
     7321 acaagggaaa gatctcactg gagaacattc cgac
//
LOCUS       DASVEU010000070         9562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_53438,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000070 DASVEU010000000
VERSION     DASVEU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53438"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(647..2311)
                     /locus_tag="VF225_02770"
     CDS             complement(647..2311)
                     /locus_tag="VF225_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144205.1"
                     /translation="MPSSGELRLRTLILALTALGGIWIALVIQRSHHVPAAAFASGPQ
                     PADFPAPKLVRVPGLNRPVSAAPVADVSPRVRPFTRVPTKQTPDVQVRLVAAKPAKPA
                     PSATVVVPQQDRSYAETPPPPLEPLAISDAQVVSLDSSTARITWKTNVPAQTQAAYGL
                     DAPTIWGQPSADSLIEHQSVLTGLEFGTTYQIYLHAIDEWNRAQTSTVTITTEGMPNR
                     SVASTNGSQIVVDNRPFFPTAVWAQCSDGFNSNINDGINLFMGDGCSTDDDSSLAARL
                     DGRAYSIVNADNTGAADGRGSIGWYYPDEWDAFLQSTVTRQDLEKSIAPPQSGRISFL
                     TLTNHFYSLANPLPEGKGMYPTLMSIPDVVGFDLYPLQVWCRPAFGDVMDAQRELGTS
                     SGGKPTFQWIEVAPMEHKCNEVKKLDPTPETVRAETWLAIAGGAGAVGYFPNHWSATI
                     GTEIARTNRQIKALSQALLAPVGTASSDAAAVRVSARTLNGATYVIAVNTTDTTLQAK
                     ITVPGIAGRSPVVVGGGQVVGADDAGFADSFGPLAARVYIIPPAGW"
     gene            complement(2347..3156)
                     /locus_tag="VF225_02775"
     CDS             complement(2347..3156)
                     /locus_tag="VF225_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144206.1"
                     /translation="MSLVFAAIAPHGTLPEAPVPGAERTHEALAELGRRFDAAAPEET
                     IVLSPHNVHVQGHFAIVLAGRLSGSLAEFDAPEVELSCPVDVELATEAIVALHNEGIP
                     VVGASFGANDPGAATAPMDWGVLIPLWFMGGRSQPQVPAVVVSPARDRPIEEHMRAGQ
                     ALARAAAASPKRIALIASADHGHAHDPEGPYGFDPAAGAYDETLVRLVQEDRLGDLLE
                     LDPAFVDAAKADSWWQLAMLHGALEDRWRGDFLSYEASTYFGMLCAAYAPN"
     gene            complement(3153..4073)
                     /locus_tag="VF225_02780"
     CDS             complement(3153..4073)
                     /locus_tag="VF225_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX7144207.1"
                     /translation="MSPTPILIDCDPGHDDAIALLLALASPEVELLGVTSVAGNQTLD
                     KTTANAIRVLEFAGRGDIPVAAGADRPLVREQFVASYVHGETGLDGPDLPPPQKEPVA
                     QHAVDFLAEKIRSSERPVTLIPVGPLTNVALLLALHPDARPARIVLMGGAIAEGNVTP
                     AAEFNIWCDPEAAARVFASGIEVTMVGLDITHKALFTQAHIGRLAGRVGEMVTELLRF
                     YDTFHRAVYNFDGSPIHDALAVAHVIDPDLVGTLERNIEIDTESELCRGRTVVDLWQR
                     TDREPNAHVGVEVDSDGFLELLVERINSLG"
     gene            complement(4111..4920)
                     /locus_tag="VF225_02785"
     CDS             complement(4111..4920)
                     /locus_tag="VF225_02785"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX7144208.1"
                     /translation="MPPEVGLTVVGSINLDLVSKVERLPRPGETLSGATLERVPGGKG
                     ANQAVAAARLGASVRMVGCVGPDPHAEEALSGLQAAGLQLDVREVDAPTGVAIILVAD
                     DGENIIVVVPGANAHVGGFTVDGNVLCQLEIPDEAVQEARGQAQWLCVNAAPARPLVV
                     QADLVVANRYEAEVVGEQSLLALTMGDEGAVLLEHGKEIARARPPRVVAIDGTAAGDA
                     FTACLVVALLEGREPEEALRRACAAGAIAASSFGAQPSLPTADEVDAILRA"
     gene            complement(4910..5425)
                     /locus_tag="VF225_02790"
     CDS             complement(4910..5425)
                     /locus_tag="VF225_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144209.1"
                     /translation="MLQDHAAEWATARRLVGQLGACEAAALAFCRLLERWARGDAYPS
                     TAGARDAALRHAADRAETALAGLDHPLDRYLLELESDRAEGRSWYGGPGAGELLEWEP
                     VLKRAGVGANPTRVAQAYLELAVLVRALQGLADMARIESVPDRSSLWAGLFDLRENLE
                     RAEADLRALAA"
     gene            complement(5471..7129)
                     /locus_tag="VF225_02795"
     CDS             complement(5471..7129)
                     /locus_tag="VF225_02795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7144210.1"
                     /translation="MPDTCRIIALGGLGEVGKNMTVYECDGAIIVVDAGLAFPRDEHL
                     GVDLVLPDFSYLRERADSIRAVILTHGHEDHVGSLPYLMREVPIPEVWATRLTLGLVK
                     SKLDEHGLLRAAELREVHPDDPPRELGPFRISFVRMAHSIPDSAAVVVETPAGRIVHT
                     GDYKLDHTPVDGLKTDVGKLAELGNRGVDLLLGDSTNAERPGFTQSERIVGEAFRQIF
                     PLREGRILVASFASNVHRMQQAVDVAVDLGRKVCIVGRSMRKNTNIARNLGYMEVPEG
                     VLVRPPELEEIPPNEQLILCTGSQGEPLSALTRIAYNDHPAIHVERGDTVIISAKPVP
                     GNELRVHDTINQLAKTGAEVLHQEIAPVHVSGHACAEELRTLLALVRPRAVMPIHGEF
                     RMLAAHGQLAREAGVPAANIVLADNGSVVELSRDGVRIVDEIKTGVTFVDGLGVGDVH
                     DVALRDRRRLSEDGVLIIVATVAASDDGTAGPTELIARGFGDGSGELLEELREEADRV
                     LQDLLRNDIIEIKLLQEHLHDELGQLVYDRTHRRPMILPVVVEV"
     gene            7187..9130
                     /locus_tag="VF225_02800"
     CDS             7187..9130
                     /locus_tag="VF225_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7144211.1"
                     /translation="MRPSRSSLVTIALGLGVLGISLSRISGVGQTAFALFLAAVILVE
                     LFEESDHERAREPMQWERFRLAAAVQIAAVIMLGPWAGALVAAAGLLAGGAFRGRAFR
                     NIAFDASAGAIAACLGGLAFEIGGGNVGRLHLLEDLIALVALALAYLTARALLLDVVR
                     GREAFDPRLVAGAGEVGLGATLALLAIGHPWDVTVLVPVAIAVQQVQLRLKSLQRETL
                     RALETFANIVDERDPSTYRHSLRVAGYVDGLARALELPFSEIDRLRWAGRLHDLGKVA
                     VDASVLRKRGQLDGVEWAAMRRHPRLSARLLQRFEFVSAQARAVELHHERMDGRGYYG
                     VGGDDLPLASHFLIVADSFDAMTTDRPYRAGLTHEAALLEIERHAGTQFHPTVAKAFV
                     AVQRGVDPAAVLSAEELGELRGATAPYRIGLAGPGELKERPELIALGGVVLALAGLGF
                     AQDWLAASGGGIAVAGLTLRALVRLRAERAAASLHRALGTGEERDRVFVRLAETVARA
                     WKHDWIALMSWDEDGLGGSIEHALGDDPLPAAALTGLLVREAESGRDAIVAAGMELGR
                     SGVVVALPLRRNNSALVGFLVVAAPKLPPRHVELALLDSLDEIGLALADRPELNGRAA
                     QDAQPLETVANERDLALARLDER"
     gene            complement(9041..>9562)
                     /locus_tag="VF225_02805"
     CDS             complement(9041..>9562)
                     /locus_tag="VF225_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7144212.1"
                     /translation="IADVSDRPIVVYNIPGRVVINIEPETIVELAEIPTVRAVKQAND
                     DLVQARRIVSETDLTLYAGDDVLIFPFLELGGIGGICVHTHVVGPQVKRMIRSFHEGD
                     LDTARQLHEELQPAYELLQVQVNPIAIKAALNLLGHGVGGLRLPLVEASEGEVALVRD
                     CLERLGVLSGAPV"
BASE COUNT         1464 a         3226 c         3289 g         1583 t
ORIGIN      
        1 cgctccagcc gaggtagagc atcgacgcga ggaacagtcc gagcgcgacg agcccgagac
       61 cgatcagctc agggtgctgc tggttcttcg ccttcccctt gcgccgcttc gtgcggacac
      121 gcgcgggcag gcgcggcttg agcgtgcgct tctttcggcg tcgcgccatg tccgcgactt
      181 cgccgtccag cctccggctc ctacgcgaaa gcttcgcttt cgctccgggt atggggacgc
      241 cgcttcgcgg gaaacgtgcg cgggcggagc ccgcgcactc cactacgtgg cttcgggtcg
      301 gcctttggag tggtccacgg gcggtggcgc cttacggcgt ccccgcccgc gtgagtccgg
      361 cctgcgctta gcccggcttc gcctggtcta agctccggcc ctcggtgttg ctgatgaacg
      421 cgcgtccaac ccggaccacg aaggctggag ggtaagacca ggcgaagccg ggcttagctg
      481 gaggcgtagt gatgcgggcg cggacgcttc agcgcccgtg cccgcacgga agggaacaac
      541 cgccgagcgc caacaccatt ggagtgcgcg ggctcggccc gcgcacgttc agcgcgaagc
      601 gccgttccca agacccgagc gaaagcgcag ctttcgctcg gagggactac cagcccgcgg
      661 gcggaatgat gtagacgcgg gccgccaggg ggccgaagct gtcggcgaac cctgcgtcgt
      721 ctgccccgac tacctgaccg ccgcccacga cgaccggaga gcggccggcg atgcccggca
      781 cggtgatctt cgcctggagg gtcgtgtcgg tggtgttcac ggcgatgacg tacgtcgctc
      841 cgttgagcgt cctggccgag acccgcaccg ccgctgcgtc ggagctggca gtccccacgg
      901 gggcgagcag cgcctggctg agcgccttga tctgacgatt cgtgcgcgcg atctccgtcc
      961 cgatcgtcgc cgaccagtgg ttcgggaagt agccgaccgc gcctgcaccg cccgcgattg
     1021 cgagccaggt ctcggcgcgc acggtctcgg gagtcgggtc gagcttcttc acctcattgc
     1081 acttgtgctc catcggcgcg acctcgatcc actggaacgt cggcttccca cccgacgacg
     1141 tgcctaactc gcgctgcgcg tccatgacgt cgccgaaagc cggacggcac cacacctgca
     1201 gcgggtacag gtcgaagccg acgacatccg gaatcgacat cagcgtcggg tacatgcctt
     1261 tgccctccgg aagcgggttt gcgaggctgt agaagtggtt cgtcagcgtg aggaagctga
     1321 tccggccgct ctgaggcggg gcaatgctct tctcgagatc ctggcgcgtc accgtgctct
     1381 gcaggaaggc atcccactcg tccgggtagt accacccgat cgacccgcgt ccgtcggcgg
     1441 ccccggtgtt gtcggcgttg acgatcgagt acgcgcggcc gtcgaggcgc gccgcgagtg
     1501 agctgtcgtc gtcggtgctg cacccgtcgc ccatgaagag gttgatcccg tcgttgatgt
     1561 tgctgttgaa accatcggag cactgggccc acacggcggt cgggaagaac gggcggttgt
     1621 cgacgacgat ctgactgccg ttcgtcgacg cgacgcttcg gttcggcatg ccctcagtcg
     1681 tgatcgtcac ggtcgaagtc tgggcgcggt tccactcgtc gatcgcatgc agatagatct
     1741 ggtacgtcgt gccgaactcg aggcccgtca agacgctctg gtgctcgatg agactgtcgg
     1801 cgctcggttg cccccagatc gtgggcgcgt cgaggccgta ggccgcctgg gtctgggcgg
     1861 gcacgttcgt cttccaggtg attcgtgcag tggacgagtc gagggagacc acctgggcgt
     1921 cggagatcgc gagcggctcg agcggcggcg ggggcgtctc cgcgtacgag cggtcctgct
     1981 ggggcacgac cacggtggca gacggtgcgg gcttagccgg cttggcggcg acgagccgga
     2041 cctgcacgtc gggcgtctgc ttcgtcggca cgcgcgtgaa cggacgcacc cgaggcgaca
     2101 cgtcggcgac cggcgcagca gagacggggc gattcagccc gggcacacgc acgagcttgg
     2161 gcgccgggaa gtcggcgggc tgcgggccgg aagcgaatgc agctgccggc acatggtggg
     2221 accgctggat caccagagcg atccagatcc cgccgagcgc cgtcagcgca aggatgagtg
     2281 tccgcagccg caattctcca ctggatggca ttaccaggac gatttcggcc tgggaagccc
     2341 cggtctttaa ttaggcgcat aggcggcgca aagcatcccg aaataggttg atgcttcgta
     2401 agagagaaaa tctccgcgcc acctatcctc gagcgcgccg tgaagcattg ccagctgcca
     2461 ccagctgtcg gccttcgcag cgtccacgaa ggcgggatcg agctcgagga gatcgcccag
     2521 gcggtcctcc tggacgagac ggacgagggt ctcgtcgtag gcccccgcag ccgggtcgaa
     2581 accgtacggc ccctcggggt cgtgagcatg cccgtggtcg gcgctggcga tcaaggcgat
     2641 ccgcttcggc gacgcggccg ccgctctggc gagggcttgt cccgcgcgca tgtgctcctc
     2701 gatcggacgg tcccgtgccg ggctgaccac caccgccggc acctgcggtt gcgagcggcc
     2761 gcccatgaac cacaagggga tcagcacgcc ccaatccatc ggggccgtcg ccgctcccgg
     2821 atcgttcgca ccaaagctcg cgccgacgac gggaattcct tcgttgtgca gcgccacgat
     2881 tgcctcggtc gcgagctcga cgtcgacggg acaggacagc tcgacctcgg gtgcgtcgaa
     2941 ctcggcgagc gagcccgaca acctgcctgc gagcactatc gcgaagtgtc cttgcacgtg
     3001 cacgttgtga ggcgaaagca cgatcgtctc ttcgggcgca gctgcgtcga agcgtcgccc
     3061 gagctcggcg agcgcctcgt gggtcctctc cgcgcccggg accggcgcct ccggcaaggt
     3121 gccgtgcggc gcgatcgcgg cgaagacgag actcacccga gcgagttgat gcgctcgacc
     3181 aacagctcga ggaagccgtc cgagtcgacc tcgacgccga cgtgcgcatt cggctcgcgg
     3241 tccgttctct gccagaggtc gaccaccgtg cgcccgcggc agagttcgga ctcggtgtcg
     3301 atctcgatgt tgcgctcgag cgtgccgacg agatcgggat cgatcacgtg cgcgacggcg
     3361 agcgcgtcat ggatcgggga gccgtcgaag ttgtagacgg cccggtggaa ggtgtcgtag
     3421 aagcgcaaca gttccgtcac catctccccc actcgcccgg cgagccggcc gatgtgcgcc
     3481 tgggtgaaga gcgccttatg tgtgatgtcg aggccgacca tcgtcacctc gatgccgctc
     3541 gcgaagacgc gcgcggcggc ctcgggatcg caccagatgt tgaactctgc agccggagtc
     3601 acgtttccct cggcgatcgc gccgcccatc agcacgattc gtgcgggacg cgcatctgga
     3661 tgcaacgcaa gcaggagggc gacgttcgtg agcgggccga caggaatcag cgtcacgggt
     3721 cgctccgagc tgcggatctt ctccgcgagg aagtcgaccg cgtgctgtgc aacaggctcc
     3781 ttctggggcg gcggcagatc gggaccgtcg agcccggtct cgccgtggac gtacgacgca
     3841 acgaactgct cgcgcacgag cggccggtcc gcgccggcgg cgaccgggat gtcgccacga
     3901 cccgcgaact cgagcactcg gatcgcattc gccgttgtct tgtcgagcgt ctggtttccg
     3961 gcgacggacg tgacaccaag cagctcgacc tcgggcgagg cgagcgcgag gagcagcgcg
     4021 atcgcgtcat cgtgcccggg atcgcagtcg atgaggatgg gtgtggggct cataggccga
     4081 cctctggcct aagccgaagg cgagggacgc tcacgcgcgg agtattgcgt ctacctcgtc
     4141 cgcggtcggc agtgacggtt gcgcgccgaa gctagaggcg gcgatcgccc ctgccgcgca
     4201 cgcgcggcgg agagcttcct ccggctcccg gccttcgagg agcgcgacga cgagacaggc
     4261 cgtaaacgcg tccccggcgg ccgtcccgtc gatcgcgacc acgcgtggtg gccgggcgcg
     4321 cgcgatctcc ttgccgtgct ccagcaggac agcgccctca tcgcccatcg tcagagccag
     4381 cagcgactgc tccccgacga cctcggcctc gtaacggttc gcgactacga ggtcggcttg
     4441 cacgacgagg ggccgtgcgg gtgcagcgtt cacgcacaac cattgggcct gcccccgggc
     4501 ttcttgcacc gcttcgtcgg ggatctccag ctgacacagg acgtttccgt ccactgtgaa
     4561 gccgcccaca tgagcattgg cgcccggaac gacgacgatg atgttttcac cgtcgtcggc
     4621 gacgaggatg atcgccacac cggtgggcgc gtcgacctcg cgcacgtcaa gctgaagtcc
     4681 cgctgcctgt agtccggaaa gcgcctcctc ggcatgggga tccgggccga cgcagcccac
     4741 catccggacg ctcgcgccga gccgagctgc ggcgaccgcc tgattggcgc ccttgccgcc
     4801 cggaacgcgc tccagcgtcg ctccgctcag cgtctcccca gggcgcggca ggcgttcgac
     4861 cttgctcacc aggtcgaggt tgatcgaccc gacaactgtg agacctactt caggcggcaa
     4921 gtgcgcgcag gtcggcctcc gcgcgctcga ggttctcgcg gaggtcgaag agtccggccc
     4981 agagcgaaga gcggtccggc accgactcga tgcgggccat gtcggcgagg ccttgcaggg
     5041 cgcgcacgag gacggcgagc tcgaggtacg cctgcgcaac acgcgttgga ttcgcaccga
     5101 cgcctgcacg cttcaagaca ggctcccatt cgagcagctc ccctgcgccg ggcccgccat
     5161 accaggagcg gccttcggca cgatcggatt cgagctcgag cagataacgg tcgagcggat
     5221 ggtcgagccc tgccagcgcg gtctcggcgc gatcggccgc atgtctcagc gccgcgtcac
     5281 gagcgcccgc cgtcgaggga taggcgtcgc cgcgcgccca gcgctccagc aggcggcaga
     5341 acgccagcgc cgcagcttcg cacgctccga gttggccgac gagacggcgg gccgtggccc
     5401 actccgccgc gtggtcttga agcacatccg cccagcctgg ctctctggct ctgatcgtcg
     5461 acatgagtgt ctaaacctct accacgaccg gaaggatcat cggcctgcgg tgggttctgt
     5521 cgtagacgag ttgtcccagt tcatcatgta gatgctcctg tagaagcttg atctcgatga
     5581 tgtcgttgcg caagaggtcc tgcagaacgc ggtccgcctc ctcgcgcagc tcctcgagca
     5641 gctcgcccga gccgtcgccg aagccgcggg cgatcagctc ggtggggccc gcggtgccgt
     5701 cgtccgaggc cgcgaccgtc gccacgatga tcaggacacc gtcctcggaa agccgccggc
     5761 gatcccgcaa cgccacgtcg tgcacgtccc cgactcccag accgtcgacg aacgtcacgc
     5821 ccgtcttgat ctcgtcgacg atccgtacgc cgtcgcgaga gagctcgacg accgagccgt
     5881 tgtcggccag aacgatgttc gcggccggca ctcccgcctc gcgtgcgagc tgaccatgtg
     5941 cggcaagcat gcggaactct ccgtgaatag gcatcacggc tctgggtcgc acgagtgcca
     6001 agagcgtgcg cagctcttcc gcgcaggcgt ggcccgagac gtggactggc gcgatctcct
     6061 ggtgcagcac ttccgcgccg gtcttcgcca gctggttgat ggtgtcgtga acgcggagct
     6121 cgttgccagg cacgggcttc gccgagatga tcacggtgtc gccgcgttcc acgtgaatcg
     6181 ccggatggtc gttgtaggcg atccgcgtga gcgcggagag aggctcgccc tggctgccgg
     6241 tgcaaaggat cagctgctcg ttgggcggga tctcctccag ctcgggcggg cgcacgagca
     6301 cgccttcagg cacctccatg tagcccaggt tgcgtgcgat gttcgtgttc ttgcgcatcg
     6361 accgtccgac gatgcagacc ttgcggccga ggtcgacggc gacgtcgacg gcctgctgca
     6421 tgcggtgcac gttcgacgcg aacgaggcaa cgaggatccg tccctctcgg agcggaaaga
     6481 tctgccggaa ggcctcgccg acgatccgct cggactgcgt gaacccggga cgctcggcat
     6541 tggtcgaatc gcctagcagc agatcgacgc cccgattgcc gagctcggcg agctttccga
     6601 cgtcagtctt gaggccgtcg acaggcgtgt ggtcgagctt gtagtcgccc gtgtgcacga
     6661 tccgtcctgc gggcgtctcc accacgacgg cggccgagtc cgggatcgag tgtgccatcc
     6721 ggacgaacga gatccggaac ggcccgagct cgcgaggcgg gtcgtcggga tgcacctcgc
     6781 gaagctccgc cgcgcgcaag aggccgtgct cgtcgagctt ggatttgacc agcccgagcg
     6841 tcagcctcgt cgcccagacc tcgggaatcg gtacctcccg catcaggtac gggagcgagc
     6901 ccacgtgatc ctcgtggccg tgcgtgagga ttacggccct gatggaatcg gcccgctcgc
     6961 gcaggtacga gaagtccggc aggacgaggt ccacgccgag gtgctcgtcg cgggggaagg
     7021 ccaggccggc gtctacgacg atgatcgcgc cgtcgcactc gtagacggtc atgttctttc
     7081 cgacctcgcc gaggccgccc agggcgatga tcctgcaggt atcaggcatt gaggggcacc
     7141 agcctccagt ctaagcgggg cgatggtttt ggccgataag ggtcgtatgc ggccatctcg
     7201 ctcctcgctg gtcacgatcg ccctcggcct cggcgtgctc ggaatctccc tcagccggat
     7261 ctccggggtc ggtcagacgg cgtttgccct cttcctcgct gcggtcattc tcgtcgagct
     7321 cttcgaggag tcggatcacg agcgggcgcg tgagcccatg cagtgggagc ggttccggct
     7381 cgctgccgcc gtgcagatcg ccgcggtgat catgctcggc ccctgggccg gcgcgctagt
     7441 cgccgccgcg ggtctgctcg caggaggagc gttccgcggc cgggccttcc gcaacatcgc
     7501 cttcgatgca agcgcggggg cgatcgccgc gtgcttgggc ggcctggctt tcgagatcgg
     7561 cggcggaaac gtcggcaggc ttcatctgct ggaggacctg atcgcgctcg tagccctcgc
     7621 gctcgcctat ctgaccgcac gggctcttct gctggacgtc gtccgtggcc gcgaggcgtt
     7681 cgatccccgt ctcgtggccg gcgccggcga ggtcggtctc ggcgcaacgc ttgctctcct
     7741 cgcgatcggc catccctggg acgtgacggt gctggtgccc gtggccatcg ccgtccaaca
     7801 ggtccagttg cggctgaaga gcctgcagcg cgaaacgctc cgcgcactcg agaccttcgc
     7861 caacatcgtc gacgagcgcg atccgtccac ctaccgccac tcgctgcggg ttgcgggtta
     7921 tgtcgacggg cttgcgcgtg cgctggagct tccgttctcg gagatagacc ggctcaggtg
     7981 ggcgggtcgg ctgcacgacc tcggcaaggt cgcagtcgac gcctcggtgc tgcgaaagcg
     8041 cgggcagctc gacggagtcg aatgggcggc gatgcgccgc cacccgcggc tctccgcgcg
     8101 tctcttgcaa cgcttcgagt tcgtctccgc gcaggcacgc gcggtcgagc ttcatcacga
     8161 acggatggac ggccgcggct actacggcgt cggcggcgat gatctacccc tcgcgtcgca
     8221 cttcctgatc gtcgcagaca gcttcgacgc catgaccacc gaccggccgt atcgcgcggg
     8281 cctcacgcac gaagccgcgt tgctggagat cgagcgacat gccgggacgc agttccaccc
     8341 gactgtcgcg aaggcgttcg tggcagtgca gcgcggtgtc gatcccgccg ccgttctctc
     8401 ggcagaggaa ctcggcgagc tgcgaggcgc gaccgccccg tacaggatcg gtctcgccgg
     8461 cccgggggaa ctgaaggagc ggccggagct gatcgcgctc ggcggcgtcg tcctcgcgct
     8521 cgccgggctc gggttcgctc aagattggct cgccgcgtcc ggcggcggca tcgccgttgc
     8581 cgggctgaca ctgcgcgctc tcgttcgcct gcgggctgaa cgggcagccg cgtcgctcca
     8641 tcgcgctctc ggcaccggag aggaacgcga ccgtgtcttc gtgcggctgg cggagaccgt
     8701 cgcccgtgcg tggaagcacg attggatcgc actgatgtcc tgggacgagg acgggctggg
     8761 cggctcgatc gagcacgcgc tcggggacga cccgcttcca gcggctgcgc tcaccggcct
     8821 gttggtgcgc gaggcggaat cgggtcgtga cgcgatcgtc gctgcgggca tggagctcgg
     8881 ccgcagcgga gtcgtcgtcg ccttaccgct gcgccgcaac aacagcgcgc tcgtaggctt
     8941 cctcgtcgtc gccgcgccga agctcccacc gcgacacgtc gaacttgccc tgctcgattc
     9001 gctcgacgag atcgggctcg ccctcgcgga tcgcccggag ctaaacgggc gcgccgctca
     9061 ggacgcccaa ccgctcgaga cagtcgcgaa cgagcgcgac ctcgccctcg ctcgcctcga
     9121 cgagcggtag ccggaggccg ccgacgccat ggccaagaag attcagcgcc gccttgatcg
     9181 caatgggatt gacctggacc tggagcagct cataagccgg ctggagctcc tcgtggagct
     9241 ggcgcgccgt gtccaggtca ccctcgtgga aggaccggat cattcgcttg acctgcggtc
     9301 cgacgacgtg cgtgtgtaca caaataccgc cgataccgcc gagctcgagg aacgggaaga
     9361 tcagaacgtc gtctcccgcg tagagcgtga ggtccgtctc gctgacgatg cgccgggcct
     9421 ggacgaggtc gtcgttcgcc tgcttgaccg cccgaacggt cgggatctcg gccagctcga
     9481 cgatcgtctc cggctcgatg ttgatgacga cgcggccggg aatgttgtag acgacgatcg
     9541 gcctgtcgct gacgtcggcg at
//
LOCUS       DASVEU010000071         4617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1219054,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000071 DASVEU010000000
VERSION     DASVEU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219054"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..2950
                     /locus_tag="VF225_02810"
     CDS             266..2950
                     /locus_tag="VF225_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144213.1"
                     /translation="MTKQPTTAEHRRLAQATGRAEDDLFTANPWYEWGPYLSERAWGT
                     VREDYSESGDAWSSFPHDQARSRAYRWNEDGMAGISDIRHELCLALSLWNGRDSILKE
                     RMFGLTGPQGNHGEDAKEYWWYLEGLPSHALLRWRYHYPQAAFPYEELIDHGRGLQDP
                     ELELLDTGVFDDDRYWSVEVTYAKASPTDVLQRIAVTNHGADEATLHVLPTLWFRNTW
                     SWSGAATRPRIERDGSGLLVANHALAGYRLEAAPGPDGSAPATLFCENETNTSRVYGT
                     EPVTPFPKDGINDHVVSALPTVNPDGVGTKAAFHYVLSVPAGDSAELRLLLHRREGKS
                     GPKKAAKKWAAGAFGRTMATREADADEFYAALAPSETTPEQMQVLRQACAGLVWSKQI
                     YPYDVRRWLDGDPGQPAPPSSRLHGRNASWRHLNSFDVLAMPDPWEYPWFAAWDLGFH
                     AVPWAHLDPAFAKYQLLVLLREWFMHPNGALPAYEWNFDDVNPPVHVMAALRVFRLDG
                     SRDREFLERVFQKLLVNYTWWVNRQDADGNNVFGGGFLGLDNISPVDRSTLPPGVKLE
                     QADGTAWMAYYTLSMLLIAIALAEENAVYQDMVIKFLEQFVMITRALDRQGLFDSEDA
                     FFYDRITPPGGEPTPVRVKTIAGLIPLLPAVALPMPTAEWAQQLGKRFARLRERFLET
                     GGSLVGRVREVNEQRTVLVSVLTPDDLKRTLREFFDEQAFLSPHGLRAVSKRYENAPY
                     TLESIPDATIDYEPAESTTTMFGGNSNWRGPVWLPLNYLAIRQFAANHHFFGEAFTIE
                     YPTGSGTQRTFGEIAQDLADRIVSIWLRGADGRRPVFGATDRIQDDPAWSDNLLFYEY
                     FHGDNGAGLGAMHQTGWTALVADLILDPPSQATPLSGA"
     gene            3125..4012
                     /locus_tag="VF225_02815"
     CDS             3125..4012
                     /locus_tag="VF225_02815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX7144214.1"
                     /translation="MRASAAAASAFQERPVAAYAACRRLLRRHDPTFYLAVLRLPADV
                     RPVVHALYGFVRGADEIVDGPGRAVEPAARLAALDAWERAFDRGLAEGTSDHPVIAAL
                     VDAGLRLELPLEELRGYMRSMRVDCDRVRIATRVELDRYMDGSAASVGRVMAAILGAR
                     QEAERFGRLGVAFQLTNFVRDVRVDYELDRIYLPAEELERFGVDAADLGLGSATPQLR
                     ALLSVEVGRARALFGESAAAVAGALPAARRGVHFAHAAYLSVLDRVEAVDYDVLAHAI
                     RPGAWNLVRAVVAGTRSRP"
     gene            complement(4195..4443)
                     /locus_tag="VF225_02820"
     CDS             complement(4195..4443)
                     /locus_tag="VF225_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144215.1"
                     /translation="MVRPSDSQEELRKDALTAFIDARVREGYRVETRTDTHAIIAPAH
                     HGRSFLDRLRKPKASARQVISVDENGEVTMRPAEPLRS"
BASE COUNT          710 a         1562 c         1594 g          751 t
ORIGIN      
        1 tcgctaaggg gacgtgcctc gggcacgaga ccaatgctag gtggctgccc ggccgaacac
       61 tcggaattcg accggatgcg agcacgagat tgcctccgtc tcaattccac gggacgactt
      121 ccgaggctca acgcggcttg acatagccgg cggccggacg tacgaacgga tctgcgtgtc
      181 ctccgcaatg ggctgtacct cgccctgtcc gcgtggggct tcggtggcgt ggcgcctgtc
      241 gacgagggca ggcagagtgt ccgggatgac gaagcagccg acgacggcag aacaccggcg
      301 gctcgctcag gcgacgggtc gcgccgagga cgacctgttc actgcgaacc cgtggtatga
      361 gtggggccca tacctgagcg agcgggcctg gggcacggtt cgcgaggatt actccgagag
      421 tggcgatgcg tggagctcgt tcccgcacga ccaggcacgg tcgcgcgcct accgctggaa
      481 cgaggatggg atggccggca tctccgacat tcggcacgag ctctgccttg cgctctcgct
      541 ctggaacggc cgtgactcga tcctgaagga gcgaatgttc ggtctgacgg ggcctcaggg
      601 aaaccacggc gaggacgcca aggagtactg gtggtacctc gaggggcttc cgagtcatgc
      661 gctgcttcgc tggcgctatc actacccgca ggcggcgttc ccctacgagg agctcatcga
      721 ccacggccgg ggcttgcagg acccggagct cgagcttctc gacacgggtg tcttcgacga
      781 cgatcggtac tggtcggtcg aggtgacgta cgcgaaggcg tcgcccacgg atgtcctcca
      841 gcgcatcgcg gtcacgaacc acggtgctga cgaggcgacg ctccacgtgc tgccgacgct
      901 gtggttccgc aacacgtggt cgtggagcgg agcggcgacg cgtccgcgga tcgagcggga
      961 cggcagcggc ctgctcgtcg cgaaccatgc gctcgcgggg taccggctgg aggcggctcc
     1021 tggccccgac gggtcggccc ccgcgacgct gttctgcgag aacgagacga acaccagccg
     1081 cgtctacggg acggagcccg tcacgccgtt cccgaaggac gggatcaacg atcacgtcgt
     1141 ctcggcgttg ccgaccgtca accccgacgg cgttggtacc aaggccgcgt tccactacgt
     1201 gctctccgtc ccggccggcg atagcgccga gcttcgactc ctgctccacc gccgagaagg
     1261 aaagtccggt ccgaagaagg ccgcgaagaa gtgggccgcc ggcgccttcg gtcgaacgat
     1321 ggcgacccgg gaggctgatg cggacgagtt ctacgccgcg ctcgcaccgt ccgagacgac
     1381 ccccgagcag atgcaggtgc tgcgacaggc gtgcgcggga ctcgtctgga gcaagcagat
     1441 ctacccctac gacgttcgac gctggctcga cggcgacccg gggcaacctg cgccaccgtc
     1501 gtcccgtctc cacggtcgca acgcgagctg gcgacacctc aactccttcg acgtgctcgc
     1561 gatgcccgac ccctgggagt acccatggtt cgccgcctgg gacctcggct tccacgcggt
     1621 gccgtgggcg cacctcgacc cggcgttcgc gaagtaccag ctcctcgtcc tgctccgcga
     1681 gtggttcatg catccgaacg gggcgctgcc tgcctacgag tggaacttcg acgacgtcaa
     1741 cccgccggtg cacgtcatgg cggcgctccg cgtgttccgc ctcgacggca gccgtgaccg
     1801 ggagttcctc gagcgagtgt tccagaagct cctggtcaac tacacctggt gggtcaaccg
     1861 ccaggacgcc gacggcaaca acgtcttcgg cggcggcttc ctgggcctcg acaacatcag
     1921 ccccgtcgac cgctcgaccc taccgcccgg cgtcaagctc gagcaggcgg acggcacggc
     1981 gtggatggcc tactacacgc tctcgatgct cctgatcgcg atcgcgctcg cggaggagaa
     2041 cgccgtgtat caggacatgg tgatcaagtt cctcgagcag ttcgtgatga tcacgcgtgc
     2101 gctcgaccgg caggggctgt tcgactcgga ggacgcgttc ttctacgacc gcatcacgcc
     2161 gccgggcggg gagcccacgc cggtcagggt gaagacgatc gcgggcctga tcccgctcct
     2221 gcccgccgtc gcgctcccca tgccgacggc cgagtgggca cagcaactgg gaaagcggtt
     2281 cgcgcgtctg cgcgagcggt tcctcgagac tggaggatcg ctcgtcggcc gcgtccgcga
     2341 ggtcaacgag cagcgcacgg tgctcgtctc ggtgctcaca cccgacgacc tgaagcgcac
     2401 gcttcgcgag ttcttcgacg agcaagcgtt cctgtcgccc cacgggcttc gcgcggtatc
     2461 gaagcgatac gagaacgctc cgtacacact cgagagtatt cccgacgcga caatcgacta
     2521 cgagccggca gagtcgacga cgacgatgtt cggcggcaac tcgaactggc gcggcccggt
     2581 gtggctacct ctgaactacc tcgcgatccg ccagttcgca gccaaccatc acttcttcgg
     2641 cgaagccttc accatcgagt acccgaccgg ctcggggacg cagcggacct tcggtgagat
     2701 cgcgcaagac ctcgccgaca ggatcgtgtc catctggctg cgcggcgcgg acggacgccg
     2761 ccccgtcttt ggcgccacgg accgaatcca ggacgatccc gcgtggagcg acaacctcct
     2821 cttctacgag tacttccacg gtgacaacgg cgccggcctc ggggcgatgc accagaccgg
     2881 ctggaccgcg ctcgtcgccg acctgatcct cgacccgccg agccaggcga ccccgctgtc
     2941 aggcgcgtag cgcgcccaca gggcgacgag gcccaccacc atgatcatcg tgagggggtg
     3001 gggtagcccc accgacctgc cccgagcctc gcgcgcccgt gcacggtcgg aggtggaggt
     3061 gttcgacgca ggggacggat ggccgacccc gcctgctcga ggtgggccag cgtacgatcc
     3121 cgcgatgagg gcgtcagcgg cggcggcgag cgcgttccaa gagcggcccg tcgccgccta
     3181 tgccgcgtgc cggcgcctgc tccgtcgcca cgaccccacg ttttatctgg cggtgttgcg
     3241 gcttccggcc gacgttcgcc ccgtcgtcca tgcgctgtac gggtttgtgc gcggcgcgga
     3301 tgagatcgtc gacgggccgg gccgcgctgt ggaacccgcc gcgcgcctcg ccgccctcga
     3361 cgcgtgggag cgcgcgttcg accgcgggct ggccgaaggg acgtcggatc acccggtgat
     3421 cgctgcgttg gttgacgcgg gtctacgtct cgagcttccc ctcgaggagt tgcgaggcta
     3481 catgcggtcg atgcgggtcg actgtgaccg tgttcgcatc gcgacgcgag tcgagctcga
     3541 ccgctacatg gacgggagcg cggcgtctgt cggccgcgta atggccgcga tcctcggagc
     3601 gcgacaagag gccgagaggt tcgggaggct cggggtcgcg ttccagctca cgaacttcgt
     3661 ccgggacgtc cgggtcgact acgagctcga ccggatctac ctccctgcgg aggagctcga
     3721 gcgcttcggc gtggatgctg ccgacctcgg cctcgggtcg gcgacacctc agcttcgcgc
     3781 cttgctctcg gtcgaggtcg ggcgggcgcg tgcgctcttc ggcgagtcgg cggccgctgt
     3841 cgccggtgcg ttgccggcgg cgcggcgcgg agtgcacttc gctcatgccg cctacctctc
     3901 cgtcctcgac cgcgtcgagg cggtcgacta cgacgtcctc gcgcacgcga tccgccccgg
     3961 cgcatggaac cttgtgcggg ccgtggttgc cggaacaagg tcgcggccgt gaccgcggcc
     4021 actcgcggga caatcgcgga gggggcggtg gcgcccgacc cccgagtcgg gcgcgcgaca
     4081 gagcgtgcga tgatgcatgc gactgtcctc tcgcctcacg agcgcgtctg accacccgcc
     4141 ggggcgaccg agcatgaatg catccggatc tctgcgatgc gaacgttcaa gcgttcagga
     4201 tcgcagcggt tcggcgggcc gcatggtcac ctcaccgttt tcgtcgactg agatgacctg
     4261 gcgtgccgat gccttcggct tgcggaggcg gtcgaggaac gagcgaccgt gatgggctgg
     4321 ggcgatgatc gcgtgggtgt cggtgcgagt ctcgacgcgg tagccctcgc gaactcgcgc
     4381 gtcgatgaaa gcggtgagtg cgtctttccg gagttcttcc tgggagtcgc tcggtcgtac
     4441 catgttcctc cgcgtgagcg atgaaccgag gcgcatcggc gcgctgagga tagccggcag
     4501 ctcgacttgc acccgactac gccgagcagc gctgcggcgt ccgggtcgcg cgttgcgttt
     4561 gccacgaagt tcgctcatca tgagcggggc ggaggctcgg ttctgcggct gcggcgt
//
LOCUS       DASVEU010000072         7342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1025980,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000072 DASVEU010000000
VERSION     DASVEU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025980"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="ftsY"
                     /locus_tag="VF225_02825"
     CDS             <1..585
                     /gene="ftsY"
                     /locus_tag="VF225_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7144216.1"
                     /translation="GTGKTTTIGKLAERLRQHGHSVVVGAADTFRAAAEEQLEIWADR
                     ANADFVGAERGADPASVAYDAIETATRNGRDIVIVDTAGRLHTQSNLMQELAKVRRVI
                     EGKLEGAPHETLLVVDATTGQNGVQQARLFGEAVGVSGVALTKLDGSAKGGIAVAIAH
                     ELGLPVKLIGVGESLEDLRPFDSHDFARALVGGS"
     gene            complement(691..900)
                     /locus_tag="VF225_02830"
     CDS             complement(691..900)
                     /locus_tag="VF225_02830"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7144217.1"
                     /translation="MANVAKVVTIIGSSPESFAKAADVAVQEAAKTLRGITGAQVVSM
                     SAEVEGDRISMYRTTVNVAFGIERS"
     gene            complement(932..1144)
                     /locus_tag="VF225_02835"
     CDS             complement(932..1144)
                     /locus_tag="VF225_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144218.1"
                     /translation="MPNVSGRQDVKKVTASHGQGWDQALDRALASASRLGKRGTFDVN
                     VEFSATIRVTNPGQIQRYIVTLSPKG"
     gene            complement(1199..4186)
                     /locus_tag="VF225_02840"
     CDS             complement(1199..4186)
                     /locus_tag="VF225_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7144219.1"
                     /translation="MAVSRRTVTVLFADVADSTPLGERLDPETLRQVMSRWFDRMSEV
                     LERHGGTVEKFIGDAVMAVFGIPDLHEDDGLRAVRAATELRTALARLNEELERELGVH
                     IGIRVGVNTGEVVAGDGTGGQMLVTGDPVNVAKRLEEAARTGEILIGDATRRIVENAA
                     LLEPRDELTVKGKTGPVVAWNVLGVIEGAPAYARRLDARLVGRTEELRTLRDTFASAV
                     AERTCRIVTVIGPAGIGKSRLASELSHGVRDEAQTLTGRCLPYGDGITFWPLVQIVGA
                     LGSDEGIRELLAGTEDGELVAERVLSAVGPTRTSAAGGEMFWAVRRLLEEAARERPLV
                     VVVEDIHWAAPTFLDLLEYLAGWTHDAPMLLLCLSRPDLLEERQGWLTAPNATGISLA
                     PLTDAESDALLDELGQEWPLDETARRQIKEAAEGNPLYVEQMAAMLAEGGPADAIPPS
                     IHALLAARLDRLPPEERAILERAAVAGKEFVRSAVLHLYDHAQREEVDAGLLTLARKD
                     LLAAKPGREDAYRFRHALIRDAAYAGIAKELRAQLHERFGDWALGTNAGRAGELDEIV
                     GYHFEQAFRYREQLGPLDDASRRLAGRAGDLLGGAGRRAFARDDMPAALTLLDRAVAL
                     ITEQDPARLELVRELSMALWWAGEVARAELLLNGLIEAAAASGDQRQEWSGLIERAAR
                     KNVSGGDTGADELLEVSREAIEVFEELGDEAGLARAWRRIAYAHQMRSQFGPAESASE
                     QALIHARKAEDSHEEARIIDGLCTSLLWGPASVPEAIQRCEEMLALSRGSRVMEANIG
                     ISLAGLKGMRGDFEAARALAAGARQTYRELGLRLAVAGLTQVSGPLEALAGDLEAAEQ
                     ELREGLEIMEPVGSIGYQAALLADVLLRRGATDESERFASIAQAEAADDNIAAQVIWR
                     GVKARLDTNPGLAEEAVGIAGLTDDLNLRADATVNLAESLRLADAQDHAVDALQRAVQ
                     LYEQKGNVVGALRASKLASAPVR"
     gene            complement(4219..>7342)
                     /locus_tag="VF225_02845"
     CDS             complement(4219..>7342)
                     /locus_tag="VF225_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7144220.1"
                     /translation="DHSERKLATVMFADLVDSTAAAGEQDPERTRARLERFYDAMSAE
                     IEGGGGTVEKFAGDAVMAAFGAPEALEDHAERALHTALAMQRRLEAVFGGELSLRIGV
                     NTGEVVAGRPREGSSFVSGDAVNVAARLEQAAEPGEILAGERTVSAVRGAFEFGEPKT
                     VEAKGKAGGVACRPLVRALSLIRTRGVGGLARAFVGRDEQLDLLQSVYRRAVEGSHPV
                     LVTIVGDPGVGKTRLVRELWEQLADERPEPLRRTGRCLAYGQGITYWPLGEVLKEHLE
                     ILESDSPETVRSRLDEREILGLTLGLDVVGDLHPLAAQDRLHAAWIAFVSELTAERPL
                     VLLIEDVHWAEQPLLDLIERIGRDAAGPLLLLTTARPDFVAGRTGWGARVDSETLWLE
                     PLPAETAGKLVDSLLASVLSRDVRELIVERAEGNPFFVEEVLESLIDAGVLERENGGW
                     RANELPPGFEIPDSVQAVLAARIDLLEEAEKTALQAAAVIGRVFWTGPVYELVAGLAP
                     DLHLLETRDFIRQRPASSLAGEVEYAFKHALTREVAYGTLTKARRAQLHTQFAEWLER
                     LGEGRDEHAPLLAHHYAEAVRPEDVDVAWPEGGPELERARAKAVLWLDRAAEAAKSRY
                     ELDDAVDLLQRAVALVSERRETALLWLKIGRAHALRYDGDGFTESMQRALELTDDPVE
                     RAEAYAVLAYHSATRSGMWRRRPERDVMEDWTSRALAGVQQGGAAHVKALLARVYFGL
                     PDADESTLLATELADQLGDVDLRSSALDGRGWTAFRHGDFAAAHEWEMRRFDFIDQLT
                     DPDLIHDLYLSTIPTAMALGRTDEARRLAAENDQLVARLTPHHRLHGVACRLEIEELI
                     GNWDAIAALEHRTEVVVEANRDTPCIRNARSLLLCALANELLGNHDRSIELEARAAEL
                     QNEGYGATLATPRARLALARGEFDQLHELLRDEGWRQRQTWFTLPAGAARLDALAVVG
                     ASAEVEAAAAQIGTPGSYLEPFALRALGIVQEDETLLAQAHERFRRFHLDWHADQTEA
                     LIQFRNTALG"
BASE COUNT         1104 a         2556 c         2450 g         1232 t
ORIGIN      
        1 ggcaccggca agaccacgac gatcggaaag ctggcggaac gcctgcgcca gcacggccac
       61 tccgtcgtgg tcggcgcggc cgacacgttc cgcgcggcgg ccgaggagca actggagatc
      121 tgggccgacc gcgccaacgc ggacttcgtc ggggcggagc gcggagcaga tccggcgtcg
      181 gtcgcctacg acgcgatcga gaccgcgacg cgcaacggcc gggacatcgt catcgtcgat
      241 accgccggcc ggctgcacac gcagtcgaac ctgatgcagg agctcgccaa ggtgcgtcgc
      301 gtgatcgaag gcaagctcga aggagcgcca cacgagacct tgctagtcgt cgacgcgacg
      361 accgggcaga acggtgtgca gcaggcgcgc ctgttcggcg aggcggtcgg ggtctcgggt
      421 gtcgcgctga cgaagctcga cggcagcgcg aaaggcggca tcgccgtcgc aatcgcgcac
      481 gagctcggtt tgccggtcaa gctgatcggc gtcggcgaga gtcttgagga cttgcgaccg
      541 ttcgattcgc acgattttgc cagggcgctt gtcggcggct cctagatcgt cagcaagacg
      601 gacgcgcgag cgaagcccgc acgtcctcca agcggcaccg cgagcggtgc ctcaggattc
      661 gcacgctttc gctcgggcgc tggtttccgg ttagctccgc tcgatcccga aagcgacgtt
      721 cacggtcgtc cgatacatgc tgatccggtc gccctcgact tctgcgctca tcgagacgac
      781 ctgcgcgccg gtgatcccac gcagtgtctt cgccgcttcc tgaaccgcca cgtccgcggc
      841 tttcgcgaag ctctcgggtg acgaaccgat gatcgtcact accttggcaa cgttggccat
      901 ttcagctcct tgtcgtcgga tgcgttgacg cttaaccctt aggcgaaagc gtgacgatgt
      961 aacgctggat ctgccctgga ttcgtgaccc ggatggtcgc gctgaactcg acgttcacgt
     1021 caaacgtgcc tctcttgcca aggcggctcg cactcgcgag cgcacgatcc agtgcctggt
     1081 cccagccctg gccatgcgaa gccgtgacct ttttcacatc ttgcctgccg ctgacgttgg
     1141 gcatcgttcc tcctgtctcg gaaacaactg gacgataacc cggtccgggc cgccgcgcct
     1201 agcgcacggg cgccgaggcg agcttcgatg ctctgagcgc tccgacgacg tttccctttt
     1261 gctcgtagag ctgcacggct cgttgcaatg catcgacggc gtggtcctgc gcatcggcca
     1321 ggcgcaatga ctctgcaaga ttcacggtcg catcggcgcg caggttcagg tcatccgtga
     1381 ggcccgcgat cccgactgcc tcctccgcca ggccggggtt cgtgtcgagg cgcgccttga
     1441 cacctcgcca gatgacctgc gccgcgatgt tgtcgtccgc tgcctcggcc tgtgcgatcg
     1501 acgcgaacct ctcggactcg tccgtcgcgc ctcggcgcag gagcacgtcg gcgaggaggg
     1561 cggcctggta accgatcgaa ccgaccggct ccatgatctc gagcccttct cgtagctcct
     1621 gctccgcagc ttcgaggtcg ccggcaaggg cctcgagcgg accggagacc tgggttaggc
     1681 ccgcgaccgc aagccgtagc ccgagctcgc ggtacgtctg ccgggcccca gccgcaagcg
     1741 cccgtgccgc ttcgaagtcg ccgcgcatgc ccttgaggcc ggccagcgaa atgccgatgt
     1801 tcgcctccat cactcggctg cctcgtgaca aggcaagcat ctcttcgcag cgttggattg
     1861 cctccggcac cgacgccggg ccccagagca ggctcgtgca aagtccatcg atgatgcgcg
     1921 cttcctcgtg gctgtcctcg gccttgcgcg catgaatgag cgcctgctcg ctggctgact
     1981 cggccggccc gaattgcgag cgcatctgat gggcgtacgc gatccggcgc caggcgcgcg
     2041 ccagccctgc ttcgtcgccg agctcctcga agacctcgat cgcctcacgc gagacctcca
     2101 gaagctcatc cgcgccggta tctccaccgg acacgttctt gcgcgctgcg cgctcgatca
     2161 gaccggacca ctcctgtcgc tgatccccgg acgcggcagc cgcttcgatc agcccgttga
     2221 gcaggagctc ggctctcgcg acctcaccgg cccaccaaag cgccatgctc agctcgcgca
     2281 ccagctcgag gcgcgcggga tcctgctcgg tgatcaatgc gacagcccgg tcgaggagcg
     2341 tgagcgcggc aggcatgtcg tctcgcgcga acgcccggcg gccggctcct ccaagaagat
     2401 cgccggctct gccggcgagc cgtcgcgatg catcgtcgag cggtccgagc tgctcccggt
     2461 aacggaaggc ctgctcgaag tggtagccga cgatctcgtc gagctcgccc gcgcgccccg
     2521 cattcgtgcc gagcgcccaa tcgccgaagc gctcgtgcag ttgcgcccgc agctccttcg
     2581 cgatccccgc gtatgccgcg tcgcgaatca gggcgtgacg gaaccggtac gcgtcctccc
     2641 gcccgggctt ggccgcgagc agatccttcc gcgccagggt gagcaggccc gcatcgactt
     2701 cctcgcgctg cgcgtggtcg tagaggtgaa ggaccgcgct ccggacgaac tccttgcccg
     2761 cgactgcggc acgctcgagg atggcgcgct cttccggcgg gagccggtcg agccgcgcgg
     2821 caagcagagc gtgaatcgac ggcgggatgg catcggcagg cccgccttcg gccagcattg
     2881 ccgccatctg ctcgacgtag agcggattgc cctcggccgc ttccttgatc tgacgtcgcg
     2941 ccgtctcgtc gagtggccat tcctggccga gctcgtcgag cagggcgtcc gactcggcat
     3001 cggtgagggg ggcgagggag atgccggtgg cgttgggcgc ggtcagccag ccctgacgtt
     3061 cttcgagtag atcgggacgc gacagacaga gcaggagcat gggtgcgtcg tgcgtccagc
     3121 cggcgaggta ctccaggaga tcgagaaacg tcggcgcggc ccagtggatg tcctcgacga
     3181 ccacgaccag cggccgttcg cgagctgcct cttcgaggag ccgccgcacg gcccagaaca
     3241 tctcgccgcc tgccgctgac gtgcgggtag ggccgactgc gctcagtact cgctccgcga
     3301 ccagctctcc gtcctcggtt ccggccagca gctcccgaat cccctcgtcg ctgccgagcg
     3361 ccccgacgat ctggacgagt ggccagaagg tgatgccgtc gccgtagggg agacagcggc
     3421 ccgtgagagt ctgagcttcg tcgcggaccc cgtggctgag ctcggacgcg agtcgcgact
     3481 tgccgattcc cgcaggaccg atgacggtga cgatccggca cgtgcgctcg gcgaccgcgc
     3541 tcgcaaacgt gtcgcgcagc gtccgcagct cctcggtgcg gccgacgagg cgcgcgtcga
     3601 ggcgacgcgc ataagcgggt gcgccctcga tcacacccag cacgttccat gcgacgacgg
     3661 gcccggtctt gcccttcacc gtcagctcgt ctcgcggctc gagcagggcg gcgttctcga
     3721 cgatgcggcg cgtcgcgtcg ccgatcagga tctcgccggt cctggctgct tcctcgagtc
     3781 gcttggccac gttgaccgga tcgccggtca ccagcatctg gccgccggtg ccgtcacccg
     3841 cgacgacctc gcccgtgttg acgccgacgc ggataccgat gtgcacgccg agctcccgct
     3901 cgagctcctc gttgagtcga gcgagcgccg ttcgcagctc ggtggcggct cggacggcgc
     3961 gcaggccgtc gtcctcgtgc agatccggaa tgccgaagac cgccatcacg gcgtcgccga
     4021 tgaacttctc caccgtgccg ccgtggcgtt cgagcacctc ggacatgcgg tcgaaccacc
     4081 gcgacatgac ctgtcggagc gtctctgggt cgagccgctc tccgagcggc gtcgaatcgg
     4141 ccacgtctgc gaagaggacg gtgaccgttc ttcggcttac ggccatgtcc tgagtgtagg
     4201 actcggttct tggagtgcct agccgagggc ggtgttccgg aactggatga gcgcctcggt
     4261 ctggtcggcg tgccagtcca ggtggaagcg ccgaaagcgc tcgtgggctt gtgcaagcaa
     4321 cgtttcgtct tcttgcacga ttccaagtgc ccggagtgcg aatggctcga ggtacgaccc
     4381 gggggttccg atctgcgcgg ctgcagcctc gacttcggcg gaagcaccga cgacggcgag
     4441 tgcgtcgagg cgagccgcgc ccgcggggag cgtgaaccac gtctgccgct ggcgccagcc
     4501 ctcgtcgcga agcagctcgt gcagctggtc gaattcgccc cgggccagtg caaggcgggc
     4561 gcgcggggtc gcgagggtcg cgccgtagcc ctcgttctgc agctcggcgg cgcgagcctc
     4621 gagctcgatg ctgcggtcgt ggttgccgag taactcgttc gcgagcgcgc agagcagcaa
     4681 cgagcgcgcg ttacgtatac acggcgtgtc gcggttggcc tcgacgacca cttcggtccg
     4741 atgctcgagc gcggcgatcg cgtcccagtt gccgatgagc tcctcgatct ccagcctgca
     4801 cgcgacacca tggagccggt gatggggtgt caaccgtgca acgagctggt cgttctcggc
     4861 tgccaggcgg cgtgcttcat ccgtcctgcc gagcgccatc gccgtcggaa tcgtcgagag
     4921 gtacagatca tggatgagat cgggatctgt gagttggtcg atgaagtcga accggcgcat
     4981 ctcccattcg tgcgcagcgg cgaagtcacc gtgacggaat gccgtccatc cccgcccgtc
     5041 aagggcggag gagcgcagat cgacgtcgcc gagctgatcc gccagctctg tggcgagcag
     5101 cgtcgattcg tctgcgtccg gaagcccgaa gtagacgcgg gcgaggagtg ccttcacgtg
     5161 ggccgcgcct ccctgctgaa cgcccgccag cgctcgcgac gtccagtcct ccatgacgtc
     5221 gcgctcgggc ctgcgcctcc acatcccgga ccgggtagcg ctgtggtacg caagcacggc
     5281 gtaggcctcc gcacgctcga ccgggtcgtc ggtgagctcg agcgcccttt gcatcgactc
     5341 cgtgaaccca tcgccgtcgt accttagcgc atgtgcgcgg ccgatcttca gccagagcag
     5401 cgctgtctcg cggcgttcgg acacgagtgc gaccgcgcgc tggaggaggt cgaccgcgtc
     5461 gtcgagctcg tatctcgact tcgccgcttc ggctgcgcga tccagccaga ggaccgcttt
     5521 cgcccgcgca cgctcgagtt cgggacctcc ctcgggccag gcgacgtcga cgtcctccgg
     5581 tctgactgcc tctgcgtagt ggtgcgcgag caagggcgcg tgctcgtcac gtccctcgcc
     5641 gaggcgctcg agccactcgg cgaattgcgt gtggagctgc gcgcgtctcg ccttcgtcag
     5701 ggtcccatac gccacctcgc gtgtgagggc gtgcttgaag gcgtactcga cctctcccgc
     5761 gagagacgac gcgggtcgtt gccggatgaa gtcgcgcgtc tcgaggaggt gaaggtccgg
     5821 cgccaggcct gcgaccagct cgtagaccgg gccggtccag aagacgcgcc cgatcaccgc
     5881 agcggcctgc agggccgtct tctccgcttc ctcgagcagg tcgatgcgtg cagcgagtac
     5941 ggcctgcacg gagtctggga tctcgaagcc gggcggcagc tcgttcgcgc gccagccgcc
     6001 gttttcgcgc tccagcacac cggcgtcgat gaggctctcc aggacctctt cgacgaagaa
     6061 agggttgccc tcggcacgct cgacgatcag ctcacgcaca tctcgcgaga gcacgctcgc
     6121 aaggagggag tcgacgagtt tgcccgcggt ctccgccggc agcggctcga gccagagcgt
     6181 ctcgctgtcg acgcgagcgc cccagcccgt tcgcccggcg acgaagtcgg ggcgcgccgt
     6241 tgtcagcagc agcagtgggc cggcggcgtc tcggccaatc cgctcgatca ggtcgagcag
     6301 cggctgttcg gcccagtgca catcctcgat cagcagaacg agtgggcgct ccgccgtcag
     6361 ttcggagacg aaggcgatcc aggcggcgtg caacctgtcc tgagccgcaa gcggatgaag
     6421 gtcgcccacc acgtccagcc cgagagtcag gcccaggatc tcgcgttcgt ccaaacggct
     6481 gcgcactgtc tcgggcgaat cgctctcgag gatctcgagg tgctctttca ggacttcgcc
     6541 caggggccag tacgtgatgc cctggccgta cgcgagacac cgtcccgttc ggcgtaaagg
     6601 ctcgggccgt tcgtctgcaa gctgctccca cagctcgcgc acgaggcgtg tcttgccgac
     6661 gccgggatcg ccgacgatcg tgacgaggac cgggtgcgat ccctccacgg cgcggcggta
     6721 cacggattga agtaggtcga gctgctcgtc ccgtccgacg aacgcgcgtg cgagtccgcc
     6781 cacgccacgc gtgcggatca gtgagagggc gcgcaccaac ggcctgcagg cgacgccccc
     6841 ggctttgccc ttcgcctcga ctgttttcgg ctcgccgaat tcgaaagcgc cccgcacggc
     6901 ggagaccgtt cgctccccgg cgaggatctc tccaggctcg gcagcctgtt cgaggcgcgc
     6961 ggcgacgttg accgcgtccc cgctgacgaa cgagctcccc tcgcgtggcc gtccggcgac
     7021 gacctcgccg gtgttgacgc cgatccggag cgacagctcg cctccgaaga cggcttccag
     7081 ccgtcgctgc atcgccaagg cagtgtgcaa ggcgcgctcg gcgtggtcct cgagcgcctc
     7141 cggcgccccg aaggcagcca tcaccgcgtc cccggcgaac ttctcgacgg ttcctccgcc
     7201 tccctcgatc tccgcgctca tcgcgtcgta gaagcgctcc agccgggcgc gggtccgctc
     7261 gggatcctgt tcccctgcgg ccgcagtcga gtcgacgaga tccgcgaaca tgaccgtggc
     7321 gagcttgcgc tcggaatggt cg
//
LOCUS       DASVEU010000073         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_54966,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000073 DASVEU010000000
VERSION     DASVEU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54966"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF225_02850"
     CDS             complement(<1..183)
                     /locus_tag="VF225_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144221.1"
                     /translation="MTTPTRAQSDLTLARDGGVPARTRPEAPMYPGGMEVGPDELEAL
                     ARVIESKNLFRYYGVGD"
     gene            complement(463..603)
                     /locus_tag="VF225_02855"
     CDS             complement(463..603)
                     /locus_tag="VF225_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144222.1"
                     /translation="MAKKKLTKAERAERDARHERVLANAQRTRELAERAQAKLDAQSS
                     RG"
     gene            complement(575..709)
                     /locus_tag="VF225_02860"
     CDS             complement(575..709)
                     /locus_tag="VF225_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144223.1"
                     /translation="MPDSLIERAEVVALLFNVSDIAITLANMERLLGEDSGEEEADES
                     "
     gene            complement(801..2663)
                     /gene="iolD"
                     /locus_tag="VF225_02865"
     CDS             complement(801..2663)
                     /gene="iolD"
                     /locus_tag="VF225_02865"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX7144224.1"
                     /translation="MPRITAAQALVRFLAAQHVERDGAEQRFFEGCFGIFGHGNVAGL
                     GQALYQHPDLLTYYQARNEQAMVHTSVGFARMKNRLATLACTTSIGPGATNLVTGAAL
                     ASVNRLPVLLLPGDVFASRRPDPVLQQLEVPSRGDVSVNDALQPVSRYFDRIERPEQL
                     LAAAPAAMRVLVNQAETGAVTLAFPQDVQAEAYDYPDEFLAHRVWHISRPEPDAKVLA
                     DAVALIRSAKRPLIVAGGGVIYSEATDALRAFAEQTGIPVGETQAGKGSLPYDHPSSL
                     GAIGATGTLAANRIAADADVVIGIGTRWSDFTTASKTAFRNPDVRYVNVNVADFDAAK
                     LAGIAVVADARVALERLAVALAGHRVDAAYSELAARLNTEWDEEVTRLYGLGHEPLPA
                     QSEVIGAVNDAARPTDVVVCAAGSMPGDLHKLWRTRDPKGYHVEYGYSCMGYEIAGGL
                     GVKMAAPDREVYVMVGDGSYLMLSSEIVTSIQEGLKLTIVLVDNHGFNSIGGLSRSLG
                     TEGFGTQYRYRQNGSLGLDRDSPTAPLLPVDLAANAESLGAKVIRCRTIDDLRGGLEA
                     AKGGASTVVLAIEVDRYEGVPGYESWWDVPVAEVSEVESVREARRAYEQARRST"
     gene            2718..>3991
                     /locus_tag="VF225_02870"
     CDS             2718..>3991
                     /locus_tag="VF225_02870"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7144225.1"
                     /translation="MAVTVRTLENFIGGEWVASTGESARVVVSPVTGETLAEAPDAGA
                     GDIARAVAAARAAQPKWAALSAWERAKVCHAIADLIDERREDFARELSLEQGKPYAAE
                     ALGDIDETAENFRIAAEDVKRMETAIIPSQDPNKRILTFRKPNGVYAGITPWNFPTLI
                     PVELIAPGIAVGNTIVMKPSEWTPIAMANFMQIMADAGLPDGVVNVVYGGGDVGERLV
                     THPDVDCVGFVGSHPTAEKIVRAAGLKRSLIEASGNGPVVVCEDADLGRAAQGAVFGG
                     FFCAGQVCCATERVLVHEGVHDAFLDEVLKAAADWRLGDPFDDSTMVGPMNNEPTASK
                     MDRHLEDAVDKGASVVLGGSRASDRPTRLYYEPTVVTDVGVDTLINQQETFGPIVPLI
                     RVRSDEEALAFANDSHLGLQAAVYTSSLKRAFR"
BASE COUNT          652 a         1365 c         1320 g          654 t
ORIGIN      
        1 gtcgccgacg ccgtagtagc ggaagagatt cttgctctcg atcacccggg ccagcgcctc
       61 gagctcgtcc ggcccgacct ccatgccgcc ggggtacatc ggcgcctcgg gccgggtccg
      121 ggccgggacg cctccgtctc gggcgagggt caggtcactt tgggcgcgcg tgggcgtcgt
      181 cataccggcc gcccattctg gacatgaaat cgtttgcgat caaggtggca agccgttcga
      241 gcccctcccg caccgggcca aaggacattc ccgcccgcag acccttccaa gttcgagccg
      301 tggctctgac cgacctgctc ctaaaggctg gggtcagacc cttgccgttc gagccgtggc
      361 tctgaccgac cgactcctaa aggccggggt cagacccttg ccgttcgagc cgtggctctg
      421 accgaccggc ttgtacggct gaggccgtcc gctctagaac ggctatccgc gagagctctg
      481 cgcgtcgagc ttggcctgcg ctcgctcggc caattcgcgc gtgcgctggg cgttcgcgag
      541 gacgcgttcg tgccttgcgt cccgctcggc gcgttcagct ttcgtcagct tcttcttcgc
      601 cactgtcttc tcctagcagt cgctccatgt tcgccaatgt gatggcgatg tcgctcacat
      661 tgaagagtaa ggcaacgact tcggcgcgct cgatgagtga gtcgggcatt tgtcacagcc
      721 tctgcgagga caggccgcat cgcaattcgc ggattcagac acggcccgac gcggactgag
      781 agcgtcgacg cgatacggga tcaagtcgac cgacgggcct gttcgtaggc tcgacgcgcc
      841 tcgcgaacgc tctcgacctc cgaaacctcg gccaccggga cgtcccacca gctctcgtag
      901 cccggcactc cctcgtagcg atcgacctcg atcgcaagca cgacggtcga ggccccgccc
      961 ttcgcggcct cgagcccccc gcgcaggtcg tcgatcgtgc ggcagcggat caccttcgcg
     1021 ccaagcgatt ccgcattcgc cgccagatcg accgggagca gcggcgccgt cgggctgtcg
     1081 cggtccaggc cgagggaacc gttttgccgg tagcggtact gggtgccgaa accctccgtg
     1141 ccgagcgagc gcgagaggcc gccgatcgaa ttgaacccgt gattgtcgac gagcacgatc
     1201 gtcagcttca ggccttcctg aatcgaggtc acgatctcgc tcgagagcat gaggtaggaa
     1261 ccgtcgccga ccatgacgta gacctcacgg tcgggagcgg ccatcttcac gccgaggccg
     1321 ccggcgatct cgtagcccat gcacgagtag ccgtactcga cgtggtagcc cttcggatcc
     1381 cgcgtccgcc agagcttgtg caggtcgccc ggcatcgagc cggcggcgca gacgacgaca
     1441 tcggtaggcc gcgcggcgtc gttgacagcg ccgatcacct cgctctgggc aggcagtggc
     1501 tcgtgaccga gcccgtacaa ccgcgtgacc tcctcgtccc actcggtgtt gagccgggca
     1561 gccagctcgc tgtatgcggc atcgacccga tgtccagcga gcgcaacggc tagccgctcg
     1621 agagcgaccc gcgcgtcggc gacgaccgcg attccagcga gtttcgcagc gtcgaagtcg
     1681 gcgacgttca cgttgacgta acgcacatcg ggattccgga acgcggtctt cgaggcagtc
     1741 gtgaagtcgg accagcgggt gccgatgccg atcacgacgt ccgcgtccgc ggcgatccgg
     1801 ttcgccgcga gtgtgcctgt cgcaccgatt gccccgagcg aagacggatg gtcgtacggc
     1861 agtgagcctt tccctgcctg ggtctccccc accgggattc ccgtctgctc ggcgaatgca
     1921 cgcagggcgt cggtcgcctc ggagtagatc acgccgcccc cggcgacgat caaggggcgc
     1981 ttcgcgctgc gaatgagcgc gaccgcgtct gccaggacct tcgcgtccgg ctcgggccgc
     2041 gatatatgcc agacgcggtg cgcgaggaac tcgtccgggt agtcgtacgc ttccgcctgc
     2101 acgtcctgcg ggaacgcaag cgtcacggct cccgtctcgg cctggttgac gagcacccgc
     2161 atcgccgccg gagccgccgc gagcaactgc tcgggccgct cgatgcgatc gaagtagcgc
     2221 gacacaggct gcagcgcgtc gttgacggag acgtcgcccc gcgacgggac ctcgagctgt
     2281 tgcaaaacgg gatcgggccg ccgcgacgcg aacacgtcgc cgggtaggag cagcaccggt
     2341 agccggttga ccgaggcgag ggccgcgccg gtgacgaggt tcgtcgcgcc cggcccgatg
     2401 gaggtggtac acgcaagcgt ggcaagtcgg ttcttcatcc gcgcgaagcc gacggaggtg
     2461 tgcaccatcg cctgctcgtt gcgggcctgg tagtaggtca gcagatccgg gtgctggtag
     2521 agcgcctgcc cgagccccgc cacgttgcca tgcccgaaga tcccgaagca cccctcgaag
     2581 aaccgctgct ccgcgccgtc ccgctcgacg tgttgggcgg cgagaaagcg aaccagcgcc
     2641 tgggcggccg tgattcgagg catgggacaa ctctctcaaa aaaggtacgg tcgccccagc
     2701 gttcgaaagg agcccccatg gccgtgacgg tccgtactct cgagaacttc atcggcggcg
     2761 aatgggtcgc ctcgaccggc gagagtgccc gcgtcgtggt ctcccccgtc accggcgaga
     2821 cgctcgccga ggcgccggac gccggcgcgg gcgacatcgc ccgcgcagtg gcggccgccc
     2881 gtgcggccca gccgaagtgg gccgcgctgt cagcctggga acgggcgaag gtctgccacg
     2941 ccatcgccga cctgatcgac gaacggcgcg aggactttgc acgcgagttg tcgctcgagc
     3001 agggcaaacc ctacgccgcc gaggcgctcg gggacatcga cgagaccgca gagaacttcc
     3061 gcatcgccgc cgaggacgtc aagcggatgg agacggcgat catcccgtct caggatccga
     3121 acaagcggat cctcaccttt cgcaagccca acggcgtcta tgcgggcatc acaccctgga
     3181 actttcccac gctcatccct gtcgagttga tcgccccggg gatcgccgtc ggtaacacga
     3241 tcgtgatgaa accctccgag tggacgccga ttgcaatggc gaacttcatg cagatcatgg
     3301 cggatgccgg cctgccggac ggcgtcgtga acgtggtcta cggcggcggc gacgtcggcg
     3361 agcgcctcgt cacgcatccg gacgtcgact gcgtcggctt cgtcggctct catccgaccg
     3421 cagagaagat cgtgcgcgcc gccggcctca aacgctctct gatcgaggcg agcggcaacg
     3481 ggcctgtcgt cgtctgtgag gacgccgatc tagggcgcgc cgcgcaaggt gccgtcttcg
     3541 gtggcttctt ctgcgcagga caggtgtgct gtgcgacgga gcgggttctc gtgcacgagg
     3601 gcgtacacga cgccttcctc gacgaggtgc tgaaggcggc ggcggactgg cgccttggcg
     3661 atccgttcga cgactcgacc atggtcggcc cgatgaacaa cgagccgaca gcgtccaaga
     3721 tggatcgaca cctggaggac gccgtggaca agggcgcaag cgtcgtcctg ggcggctcgc
     3781 gagccagcga tcgccccact cgtctctact acgagccgac cgtcgtcacg gacgtcggcg
     3841 tcgacacgct catcaaccag caggagacgt tcggcccgat cgtgcctctg atccgcgtcc
     3901 gctctgacga ggaggcgctc gcgttcgcga acgactcgca tctcggcttg caggcggcgg
     3961 tctacacctc gagcctgaaa cgcgcgtttc g
//
LOCUS       DASVEU010000074         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_251527,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000074 DASVEU010000000
VERSION     DASVEU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251527"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..344
                     /locus_tag="VF225_02875"
                     /pseudo
     CDS             1..344
                     /locus_tag="VF225_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            792..1343
                     /locus_tag="VF225_02880"
     CDS             792..1343
                     /locus_tag="VF225_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144226.1"
                     /translation="MVNEARLEELDSGLAPVTDGWFVVNVRDAAWETNDHFGAMCAFE
                     GERAPFADLGINLRVLRPGGRGLYHAESTQEDFLVLAGQCLLLVEGEERPLTAWDFVH
                     CPPGTEHAFVATADGPCIIIMAGAPRSSSAGTLAYPRSELALAHGVGVETETRSPREA
                     QSQLGIPAWRDRRPDDWNALPWA"
     gene            1441..2280
                     /locus_tag="VF225_02885"
     CDS             1441..2280
                     /locus_tag="VF225_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144227.1"
                     /translation="MASTKTASHLFARNPEKVRRALRELDAGELLPALGARLERALEG
                     SDDATREYVRCFNVLTLRVLDEAHRVRPDIIVRTDEGDLAVIDVKGTPRRAPVEALET
                     VSAEAPFSPWTAWFPLEEAVALDLTSRVCLLIGLRGPDVVPAGSGVGAVVDDLTAHHF
                     LRRVRFYLNHPDQEHPLRRVMDAFELSKTELASLFGVRRQAVDQWLERGVPSERQEKV
                     QTLVAICDLLERKLKPGRLVGVARRPADAYGGKTMLNLIAADRHRELLELVRDSFDWA
                     SAA"
     gene            2285..2833
                     /locus_tag="VF225_02890"
     CDS             2285..2833
                     /locus_tag="VF225_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX7144228.1"
                     /translation="MAYGHTIRRGGSYNRLAEPSWSDPLDTSYSRQRGGRWNIPGSFG
                     VLYLNRDLRVARLQVQHKLRGQPYGVEDLDESEQHHLVTVEVDERDWLDCVTGPGLEA
                     VGLPATYPRHRNGRPVQHAACQPIGRSAFEDGRPGIASRSAATGASQTDEELGVFDRD
                     VTTSLRMTDRQQFADWFWGSQS"
     gene            complement(2908..3822)
                     /locus_tag="VF225_02895"
     CDS             complement(2908..3822)
                     /locus_tag="VF225_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7144229.1"
                     /translation="MKRRWMRELADFQRFCTSERRLAPMTCAAYERDVSACLDYLQGQ
                     GIRDLGAVKVKHLRAFLAEEQKRRPAVSSQARTTAALKCFFRFLVEEERIVRNPALPL
                     RTPKKRESLPDTLTMSELERLLAQPGRDDVWERHFPGKPERDRLLLALMAYAGLRRSE
                     LLGLEWDDVDLSRRLLRVRKAKGGRERTIPIHSALAPLFAEYYATRVPLTAQAVFLGV
                     QGKPLNYTQLGQVFRHYVSAARVNERKLVTPHTLRHVFASELLRAGANLRQIQELLGH
                     KHLDSTQRYTRVNAHELRGAIKRLRWVG"
     gene            complement(3952..>4150)
                     /locus_tag="VF225_02900"
     CDS             complement(3952..>4150)
                     /locus_tag="VF225_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144230.1"
                     /translation="GRSIATRYATIALSLFCWASSRRSQSSRACRSERPPADGRTGGG
                     HAGDRAERFHGDRSQPVAGRA"
BASE COUNT          652 a         1404 c         1420 g          674 t
ORIGIN      
        1 ttcgtcgctc cgagctgtgc tcgctcgagt ggcgcgacct caaactcaac ggacgaaagc
       61 ggtcgctgct cgtctgccac gggaagggcg gcaaggctcg acgtcaaccg ctgccggctc
      121 gcctcgccca tgagctgctg aagctcagcg acgcacgcga gcccgcgccg accgacccgg
      181 tcttctgcgg tctcgccggc ggacgcctgc aagagacgat cctcgccgac atcatccggc
      241 gagccgcgaa gcgcgccggg gtcgagaagc acgtcaccgc gcacacgctg cgccatacgg
      301 ctgcgacctg gctccggcag gagctcggcg acacgcgcct cgtagccgaa tacctcggcc
      361 acgccgacct ctcgaccgtc gcacgctatg cacacgtcga ccgcgacgag ctgttcgctg
      421 cggcggaccg actggaggtg ctcgtcgccc cggcagaaga atcggaggct tccgtcgatg
      481 cccagtcggc aggtgacagt cggccggatc acgcatcgca gtccagcgac gcccgcgacg
      541 acgacgctat ccagtcgcaa ccaccgggtc gtcggcggcg gcgtcgcggg aggcgccgcc
      601 gccgttgagc ggtcaacctg ttgcgatcaa tccacaccct ctccggtcgc gagaaacggg
      661 gtcgatgacc accacgccgg tcgactcagc gaaacccgcg tacgcgcatc atcggtgcgt
      721 catcgccgga ggctagaggc ttaatgtgaa ttccggcgcg taggctgagc gacgcgagga
      781 gaggagcgga aatggtcaac gaagcacgcc ttgaagagct ggactccggt ctcgcgcccg
      841 tgaccgacgg ctggttcgtc gtcaacgttc gggatgcggc gtgggagacg aacgaccact
      901 tcggcgccat gtgcgccttc gagggcgagc gtgcgccgtt cgccgacctc ggaattaacc
      961 tccgcgttct ccggcccgga gggagagggc tgtatcacgc tgagtccaca caggaggact
     1021 tcctcgtcct ggccggtcag tgtctgctac tcgtcgaggg cgaggagcgg ccgctcacgg
     1081 catgggactt cgtgcactgc ccgccgggaa ccgagcacgc gttcgttgcg accgcggacg
     1141 gcccgtgcat catcatcatg gctggcgcgc cgcgctcatc gtccgcgggc accctcgcct
     1201 atccgcgctc cgaactcgcc ctcgcccacg gagtaggcgt cgagactgag acgagatcac
     1261 ccagagaggc gcaatcccag ctcggaatcc cggcatggcg cgaccggcgg ccggacgact
     1321 ggaacgcatt gccctgggcg tagcagcgtg caagcgaagg cgagtttcac atcaagcctc
     1381 tagcagttta ccttgcgttt ggcttgcgct tgcggtttcc ttgcgctatc gtgcgtgaac
     1441 atggcgtcca ctaagaccgc gtcacacctg tttgctcgga acccggaaaa ggtccggcgc
     1501 gctctgcgcg agctcgacgc cggcgagctt ctgccggcgc tcggagcccg gcttgagcgt
     1561 gctcttgaag gctcggacga tgcgacgcgc gagtacgtcc gttgcttcaa cgtgctgacg
     1621 ctgcgcgtgc tcgatgaggc tcatcgcgtt cgcccggaca tcatcgtccg cacggatgag
     1681 ggcgatcttg cggttatcga tgtgaaggga acgcccaggc gggcaccggt ggaggcgctc
     1741 gaaacggttt cggcggaggc gccgttcagt ccgtggacgg cctggtttcc gctcgaggag
     1801 gcggtcgcgc ttgatctgac aagccgggtt tgcctgctga tcgggctgcg gggtcccgac
     1861 gtggtgccgg cagggtccgg cgtgggcgca gtcgtcgatg atctcactgc ccaccacttc
     1921 ctacgccgcg tccgcttcta cctcaatcat cccgaccagg agcacccgct gcggcgggtg
     1981 atggacgcct tcgaactgtc gaagaccgag ctcgcctcgc tcttcggggt gcgccggcag
     2041 gcggtcgacc agtggctcga gcgtggcgtc ccgtccgagc ggcaggaaaa ggtgcagacg
     2101 ctggtggcga tctgcgacct gctggagcgc aagctcaagc caggccggct cgttggagta
     2161 gcgcgtcgcc ctgccgacgc ctacggcggg aagacgatgc tgaacttaat tgccgctgat
     2221 cggcaccgcg agctgctgga gctcgtgcgc gacagcttcg attgggcctc ggccgcctga
     2281 gcgggtggcc tacgggcaca cgatccgccg gggcggctcc tacaaccgcc tggccgagcc
     2341 gtcgtggtcg gatccgctcg acacgagcta ctcgaggcag cgcggcgggc ggtggaacat
     2401 cccggggagc ttcggtgtcc tctacctcaa ccgcgatcta cgggtggcgc gcctccaagt
     2461 ccagcacaag ctgcgcggac agccgtacgg ggtcgaggac ctcgacgagt ccgagcagca
     2521 ccatctcgtc accgtcgagg tcgacgagcg cgactggctc gactgtgtga ccggtccggg
     2581 cctagaagcg gtcggccttc cagcgaccta ccctcgtcac cgaaacggcc ggccggtgca
     2641 gcacgcggcg tgccagccga tcggccgatc cgccttcgag gacgggaggc cgggaatcgc
     2701 ctctcgctcc gccgccaccg gtgcctcgca gaccgacgag gagctcggag tcttcgaccg
     2761 agacgtgacc accagcttgc gcatgaccga cagacagcag ttcgcggact ggttctgggg
     2821 ctcacagtcg taaacgacgg tcgcgcccgg ttgggcgcgg ctcccggcgt tggtgacatc
     2881 gcatgtcggt gaccttcgag gtcaccatca gccgacccaa cggagcctct tgatcgcgcc
     2941 gcgcagctcg tgtgcgttca cgcgcgtgta gcgctgcgtc gagtcgaggt gcttgtggcc
     3001 gaggagctct tggatctggc gcaggttcgc gccggcgcgc aggagctcgg aggcgaagac
     3061 gtgccggagc gtgtgcggcg tgacgagctt gcgctcgttg acgcgagccg cgctcacgta
     3121 gtggcggaag acctgcccga gctgggtgta gttgagcggc ttcccttgga ccccgaggaa
     3181 gaccgcctgc gcggtcagcg ggacacgggt cgcgtagtac tcggcgaaga gtggtgcgag
     3241 cgcagagtgg atcgggatcg ttcgctctcg gccgcccttc gccttccgta cccgcagaag
     3301 ccgccgggaa aggtcgacgt cgtcccactc gaggccgagc agctcgctcc ggcgcaggcc
     3361 cgcgtaggcc atgagggcga gcaagagccg gtcgcgctcc ggcttgcccg ggaagtgccg
     3421 ctcccagacg tcgtcgcgcc cgggctgggc gagcagccgc tcgagctcgc tcatggtgag
     3481 cgtgtccggg aggctctccc gcttcttcgg cgtccgaagc ggcagcgccg ggttgcggac
     3541 gattcgctct tcctcgacga ggaagcggaa gaagcacttc agcgcggcgg tcgttcgagc
     3601 ctggctcgac acggccggcc gacgcttctg ctcctcggcg aggaaggcgc gcaggtgttt
     3661 caccttcacg gcgccgaggt cacggatccc ctgaccctgt aggtaatcca ggcacgccga
     3721 aacgtcacgc tcgtacgccg cgcacgtcat cggggcaagc cgccgctccg acgtgcaaaa
     3781 gcgctggaaa tcggccagtt ccctcatcca tcgtctcttc atcgctcctc ctctgggcag
     3841 cctcgcggcc cccgctggag cgaccctgag gactatccgg cttacaggcc tgctacggaa
     3901 aaggccccgg aaacggggct ttttctatgg acccgattcg ccggcgactc gtcaggctcg
     3961 ccccgcgacg ggctgactgc ggtctccgtg gaaccgctcg gcgcggtccc cggcgtggcc
     4021 cccgcccgtt cggccgtcgg caggcggtct ctcgcttcgg caggcgcgcg acgactggct
     4081 gcgtcggctc gaggcccagc agaagagcga gagcgcgatc gtggcgtacc gggtcgcgat
     4141 cgaccgacct
//
LOCUS       DASVEU010000075         8944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1416496,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000075 DASVEU010000000
VERSION     DASVEU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416496"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF225_02905"
     CDS             complement(<1..420)
                     /locus_tag="VF225_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7144231.1"
                     /translation="MKRREVVFVDGVRTAFGRAGPQGIFWKTRADDMAVKVVRELLRR
                     NPEIPPESIGDVIYAATAQVGDQGLTLGRDVALLAGISQSVPGFAVDRMCAGALTAVT
                     AGAGEIAYGACDIVLAGGVEHMGHHPMGEDVDFNPRFV"
     gene            complement(423..998)
                     /locus_tag="VF225_02910"
     CDS             complement(423..998)
                     /locus_tag="VF225_02910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX7144232.1"
                     /translation="MKTSFYDLPLLTAPGQVFTPRPSTERLVDEALERIDGVPMRVAD
                     VGTGSGAVAIALAVNRPQIQVWATDTSVAAVELARENAKLHGVEDRVHVVQGDLLEPL
                     TEPVDLVVANLPYLSEQARDPRYDNEPPEAVYAPGDGLEPYRRLLNACRDGKLETGGS
                     LLIQFHRDALAANCWELEDLREKLEASSVDS"
     gene            1010..1666
                     /locus_tag="VF225_02915"
     CDS             1010..1666
                     /locus_tag="VF225_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144233.1"
                     /translation="MVDWAEHARREDERYRDGEARLPDAEDQDARQRQLTRMGNAAGG
                     AGLARLMAGNTKDARDWFARAVERYRESYELAPPGSWGRPIAILKARILADDWEGAEA
                     DARWTLEEGAAEEQSPIGRYAATLALLVLGRDQDARVLADGLRTHDDFPAPVGDALAF
                     IAAQDPVAYVEAIEAVLESFEAREDYLEDIPAADTVVVLQALAARRELDAELSSPLLP
                     "
     gene            complement(1663..2178)
                     /locus_tag="VF225_02920"
     CDS             complement(1663..2178)
                     /locus_tag="VF225_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7144234.1"
                     /translation="MQETLVLDKPEQLKALGHPLRVRVLEMLGQEGDWQLTNRELAQR
                     LGVDPGHLHFHVRMLLKAGLIELAEANGRGREKPYRAVAKIFRVAPELLSAGGASDIQ
                     AAMIDQVQRAHAVYSADGTFRSAQLELKLTMKRALELMGTFLETARAREDEGADKIVL
                     TMFAHPPAQQD"
     gene            complement(2235..2921)
                     /gene="pgl"
                     /locus_tag="VF225_02925"
     CDS             complement(2235..2921)
                     /gene="pgl"
                     /locus_tag="VF225_02925"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7144235.1"
                     /translation="MSDDVDLVIVEDANEVAAAVAERLAHAARAGGHIALTGGTTPEQ
                     AYKEAAEREPDWSKTEVWWSDERCVPPDDDLSNYALAKRTLLDRLEHYPRAVHRIKGE
                     LGKERAAADYELELGETLFDLELLGLGTDGHVASLFPNAPTLQLSRRVLPAEPGLEPF
                     VDRVTLSIPALNSARGVLFAVSGAEKAEAARRAFASKPSPDTPASLVRARGGTTTAIL
                     DRAAAAKLGA"
     gene            complement(2918..3739)
                     /gene="proC"
                     /locus_tag="VF225_02930"
     CDS             complement(2918..3739)
                     /gene="proC"
                     /locus_tag="VF225_02930"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7144236.1"
                     /translation="MAANRKIAILGAGRIGESLLAGLLSSGWRKPNDIVVTGRREERL
                     KEVADRHGVRATLSNPEAVADAGLVVIAVKPQDFDVLLGEIGGLLTPEQTVLSVAAAI
                     PTAQIEARLAPGVPVVRAMPNAPALVHEGIAGICAGAHAADEHLDLAEDVLAHLGSVV
                     RVPERYMDAVTAVSGSGPAYFALLAEAMIDAGILLGLSRETSTQLVVQTMLGTAKLLR
                     DEEMHPVELREAVTSPGGTTIHAIRELEQAGVRAAFLNAIQAAMERSKELAAGDS"
     gene            complement(3748..4248)
                     /locus_tag="VF225_02935"
     CDS             complement(3748..4248)
                     /locus_tag="VF225_02935"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX7144237.1"
                     /translation="MIELILGDITEQGVDAIVNAANPGLLGGGGVDGAIHRAGGPSIL
                     EECRLLGGCEPGDVKATSGGALPARYVLHAVGPIWRGGGAGEPELLASCHRRAIELAD
                     ELSCRSVAFPAISTGAYGYPLGLAAPVAIAATEAALTAHRSVELARFVFRDKKTLGPY
                     RTALTS"
     gene            complement(4245..5132)
                     /locus_tag="VF225_02940"
     CDS             complement(4245..5132)
                     /locus_tag="VF225_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144238.1"
                     /translation="MTLERVVRPAGPYTLALCAKHASDATRRVRDGTLTATLRVGELA
                     EVATARQTLDGRLVLRAESEEGLERLRFVLAVDADHTEFLRRFARDPLIGETASRFQG
                     MRQLRMATVAQTLLRAFCGQLIDSRRARQLERIIVRAVEPRVDGTDLHAPPTAKSLAR
                     FAPVRLRQLGLHASRAAALVRICRSTDLEKLHALPTEQAAAFIERQRGLGPWSAGVVC
                     LEGLGRYERGLVGDLSLVKLMSRLRGRWVEAHETAELLAPYGEWGGLAGLYLITGFAH
                     GLIPLPEERPVRFPRPAYA"
     gene            complement(5129..6442)
                     /locus_tag="VF225_02945"
     CDS             complement(5129..6442)
                     /locus_tag="VF225_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144239.1"
                     /translation="MSETTEGVGARLKRLRVARGFSQRDLSSPGVSYAYISRIEAGAR
                     TPSVKALRKLAQKLGVSVEYLETGRDIREVDDRELKLADAELQLRLDSDTGAAEEKLQ
                     EVLADASAAGDRVSASRAAIGLGLAAAHRGNHLDAVERLESALEYDRVAPHLRPDVYT
                     GLAQSYAALGAPDRAARLLEQCLAEVTAATPDDVTLLVRYSTFLSYALTDAGDYGRAT
                     EVVRGALSRAGDSGDGYTRVRLYWSLARVAAIEGRSAEALTQIRKAIALLEATDDTLT
                     LARGYLLSAGIDSEEGRADETRNQLEIVARLLGPTPEPADLGMLRIGQSRLAALEGDS
                     ARAVDRARDALAILGNFHGGEMGAACWALARGLALQGETDAANETYRRAVDLLTVHGR
                     RHNAGLASLDWASLLQEQGRAEEAEPILRRAYDLGVDAELATRKS"
     gene            6660..8087
                     /locus_tag="VF225_02950"
     CDS             6660..8087
                     /locus_tag="VF225_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7144240.1"
                     /translation="MASGLAVAVYGLLLLPGAVLVWRRPVFALYAWIVGLAAHNAVMA
                     ALYGAGVRGGALTAIQAWKEILLGVALARVALEALRAGRLAFRVQPADVVAVAFAAVV
                     CVYAVLPQDWLGGTADRSAIGLALKHDLVPVGAYFLGRSVILRSEELVRLVWTVVGAA
                     AAVAVLGLLDDFFIPISWWRDSAVVDYFHKHLGYDYHGTGGLPENFVFNNGGDAHGFY
                     RRLVSTFLGPLASAYMFVVALLLGAAFLRRPRILVPLSAAIFAGLLFTFSRSSLLALA
                     AGLVVLAFVTRRWWPVAAAVATLAVSVAWVHVFPHVAPTGKWTQQDLVEQRNHAKAQP
                     GASGSAGSIDEPSIHSHWISLREGVRTVVHHPQGYGLANAGQTASRTGTPIKAGESNY
                     TEIGAETGLFGALLWIAWGLAIFVGLVAAARHTRWWAASGIAAAFAAGLVLAVQTDVI
                     GDPWMAYCLWACAGIALMSLPILRE"
     gene            8084..>8944
                     /locus_tag="VF225_02955"
     CDS             8084..>8944
                     /locus_tag="VF225_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144241.1"
                     /translation="MSATAQIPETQQRVRVIFGALILVFLLASLDQTIVSTALPTIVG
                     ELGGLQHLSWVVTAYLLASTVSGPLYGKFGDLYGRKVVLQTAIVIFLVGSALCGLSQN
                     MAELISFRALQGLGAGGLIVTAIAVIGDVIPPRDRGRYQGIFGGVFGVSTIIGPLLGG
                     FFVDNLSWRWIFYVNLPIGAVAFVVIGAVFHVRPAPKEHKVDYLGAALLAGGLSAIVL
                     FTSLGGTTWAWDSPEIIALIAIGVVAIGAFVLVESRVAEPILPLSLFRNRTFAVTSAV
                     GFVVGLALFGA"
BASE COUNT         1295 a         3088 c         3032 g         1529 t
ORIGIN      
        1 aacgaagcgc ggattgaagt cgacgtcctc gcccatcgga tggtgtccca tgtgctcgac
       61 gccgccggcg agcacgatgt cacatgctcc gtacgcgatc tcacctgcgc cggcggtcac
      121 agccgtcagc gcgccggcac acatacggtc gaccgcaaag ccgggaacgg actgagaaat
      181 gccggccagc agtgcgacgt cgcggccgag cgtcagcccc tggtcgccga cctgcgcggt
      241 ggccgcgtag atgacgtcgc caatgctctc gggcgggatc tccggatttc gccgcaacag
      301 ctcgcgcacg accttcaccg ccatgtcgtc ggcgcgcgtc ttccagaaga tgccctgtgg
      361 acccgcgcgc ccgaaagccg tgcgcacgcc gtcgacgaac acgacctccc gacgcttcat
      421 ctctacgagt ctacggagga tgcttcgagc ttctcgcgca ggtcctcgag ctcccagcag
      481 ttcgccgcca acgcgtcccg atggaactgg atcaggagcg agccgcccgt ctcgagcttg
      541 ccgtcgcggc aggcattcaa caggcggcgg tacggttcaa gcccgtcgcc gggcgcgtag
      601 acggcctccg gcggctcgtt gtcgtagcgc gggtcccgcg cctgctcgga gaggtacggc
      661 aggttcgcga caaccagatc gacgggctcc gtcagcggct cgaggagatc tccctggacc
      721 acgtgcacgc ggtcctcgac accgtgaagc ttcgcgttct cgcgcgcgag ctcaaccgcg
      781 gccacgctgg tgtcggtcgc ccagacctgg atctgcggcc ggttgaccgc cagcgcaatc
      841 gccacggcgc cgctgcccgt cccgacgtcc gcgacacgca tcgggacgcc gtcgattcgc
      901 tcgagcgctt cgtcgacgag ccgttccgtc gacggtcggg gagtgaacac ctgtccgggg
      961 gcggtcagca gcgggaggtc gtagaaggac gttttcatag gctcttgccg tggtggactg
     1021 ggcggagcac gcacggcgtg aagacgaacg gtaccgggac ggtgaggcgc gactgccgga
     1081 cgccgaggat caggacgccc gccagcgcca gctgactcgg atggggaacg cagccggagg
     1141 cgcgggtctc gcacggctca tggccggcaa tacgaaggac gcgcgcgatt ggttcgcaag
     1201 ggccgtcgag cgttaccgcg agagctacga gctcgcgcca cctggaagct gggggcgccc
     1261 gatcgcgatc ctcaaggcac gcatcctcgc cgacgactgg gaaggcgccg aggccgacgc
     1321 gcgctggacg ctcgaggagg gtgccgcgga ggagcagtcg ccgatcggcc ggtacgccgc
     1381 cacgctcgct ctgctcgtgc tcggccgcga ccaggatgcg cgcgtcctcg cggatggcct
     1441 gcgcacgcac gacgacttcc ctgcgcccgt cggagacgcg ctcgccttca tcgccgccca
     1501 ggacccggtc gcgtacgtcg aggcgatcga ggccgtgctc gaatcgttcg aggcacgcga
     1561 ggattacctc gaggacattc cggccgcgga cacggtggtc gtgctgcagg cgctcgccgc
     1621 ccgtcgcgag ctggacgcgg aactgagctc gccgctcctt ccctagtcct gctgcgcggg
     1681 cgggtgcgcg aacatcgtca atacgatctt gtccgcgcct tcgtcttcgc gggcgcgtgc
     1741 ggtttccagg aacgtcccca tcagttcgag cgcgcgtttc atcgtcagct tcagctcgag
     1801 ctgcgcgctt cgaaacgtgc cgtcggccga gtagaccgcg tgcgcacgtt ggacctggtc
     1861 gatcatcgcc gcctggatgt cgctcgcgcc gcccgccgag agcagctcgg gcgcaactcg
     1921 gaagatcttt gcgacggcgc ggtacggctt ctcgcggccg cgtccgttcg cctctgcgag
     1981 ctcgatcagc cctgccttga gcagcatgcg cacatggaag tgcaggtggc ctgggtcgac
     2041 gccgagccgc tgggcgagct cacggttcgt gagctgccag tcgccttcct ggccgagcat
     2101 ctccagcacg cgcacccgga gcggatggcc gagcgccttg agttgctccg gcttgtcgag
     2161 taccagcgtc tcttgcaaaa gaacggccgc ccctttggtc tcccggcgca cttctacata
     2221 tagagagtgc cgtgtcaagc tccaagcttg gctgctgcag cgcgatcgag gattgcagtc
     2281 gtcgtgccgc cgcgagcccg gacgaggctg gccggcgtgt cggggcttgg tttcgacgcg
     2341 aatgcccggc gcgcggcctc cgctttctcg gcgccggaga cggcgaagag gacccctcgt
     2401 gcgctgttca gagcggggat gctcagcgtt acacggtcga cgaagggctc cagcccgggc
     2461 tctgccggaa gcacccgcct gctcagctgc agggtcggtg cattcgggaa cagggatgcg
     2521 acgtgaccgt ccgtgccgag accgagcagc tcgagatcga acaaggtttc gccgagctcc
     2581 agctcgtagt cggcggccgc tctctctttc ccgagctcgc ccttgatgcg atggacagcg
     2641 cgcggatagt gctcgagccg gtcgagcagc gtgcgtttcg caagcgcata gttcgaaagg
     2701 tcgtcatcgg ggggaacgca gcgttcgtcg ctccaccaga cctccgtctt gctccagtcg
     2761 ggctcgcgct ccgccgcttc tttgtaggcc tgctcgggcg tcgtgccacc ggtgagcgcg
     2821 atgtgtccgc ctgcacgcgc ggcgtgggcc agccgttcgg cgaccgccgc agccacttcg
     2881 ttcgcgtcct cgacgatgac gaggtcgacg tcgtcgctca cgaatcgcca gccgcgagct
     2941 ctttcgatct ctccattgca gcctgaatcg cattcaagaa tgcagcgcgc acgccggctt
     3001 gctccagctc gcggatcgca tgaatcgtgg tgccgcccgg cgacgtcact gcttcgcgca
     3061 gctccaccgg gtgcatctcc tcgtcgcgca ggagcttggc cgtgccgagc atcgtctgga
     3121 cgacgagctg cgtcgaggtc tcccgcgaca ggccgagcag gatgccggcg tcgatcatcg
     3181 cttccgccag gagcgcgaag tacgcagggc ccgagccgga cacggcggtg acggcgtcca
     3241 tgtagcgctc cggtacgcgg acgacgctgc ccagatgtgc gagcacgtct tctgcgagat
     3301 cgagatgctc atcggccgca tgcgcgcccg cgcagatgcc ggcgatgccc tcgtgcacga
     3361 gcgccggcgc gttgggcatc gctcgcacga ccggaacccc gggtgccagc cgcgcctcga
     3421 tctgtgcggt tgggatggct gcggcaacgg agagaacggt ctgctcgggc gtgagtaacc
     3481 cgccgatctc gccgagcaac acgtcgaagt cctggggctt gaccgcgatc acgaccagcc
     3541 cggcatcggc gaccgcctcg gggttggaca gggttgccct gacgccgtgg cggtcggcga
     3601 cctccttcag tcgctcctca cgccgcccgg tgacgacgat gtcgttcggt ttccgccagc
     3661 ccgagctgag cagccccgcc aacagggact cgccaatccg tccggcgccg agaatcgcga
     3721 tcttccggtt cgctgccatg tcgcagtcta ggaagtcaga gccgtccggt acgggccgag
     3781 cgtcttcttg tcgcggaaga cgaagcgcgc caattccacc gaccgatgcg ccgtcagggc
     3841 cgcctctgtc gccgcgatcg caaccggggc cgccagcccc aacgggtacc cgtacgcacc
     3901 agtcgagatc gccgggaacg ccaccgatct gcagctcagc tcgtcggcga gctcgatcgc
     3961 gcgccgatgg cacgacgcga gcagctcggg ctcgcccgcg ccgccgccgc gccagatcgg
     4021 gccgacggcg tggagcacgt acctcgccgg cagcgccccg cctgaggtcg cctttacgtc
     4081 tcccggctcg cagcctccga gtaggcgaca ctcctccagg atcgacggcc cacccgcgcg
     4141 gtggatcgcc ccgtccacgc cgccgccgcc cagtagtccg ggattcgccg cattgacgat
     4201 cgcgtcgacc ccctgctccg tgatgtcgcc gaggatgagc tcgatcacgc gtacgccggc
     4261 cgcgggaaac ggacgggccg ttcctcgggc agcggaatca acccgtgcgc gaagcccgta
     4321 atgaggtaca gcccggccag gccgccccat tcgccgtagg gcgcgagcag ctcggccgtc
     4381 tcgtgcgctt ccacccagcg gccgcgcagt cgcgacatca gctttacgag cgagagatct
     4441 ccgaccaggc cgcgctcgta gcgtccgagc ccctcgaggc agacgacgcc ggcggaccag
     4501 gggccgagcc ctcgctggcg ctcgatgaag gccgccgcct gctccgtcgg cagcgcgtgc
     4561 aacttctcga ggtcggtgga ccgacagatc cggacgaggg cagccgcgcg gctcgcgtgc
     4621 agtccgagct gccgtagtcg caccggcgcg aaccgcgcaa gcgacttcgc cgtcggcggc
     4681 gcatggagat cggtgccatc gacgcgcggc tcgacagcgc gcacgatgat gcgctcgagt
     4741 tgccgcgctc ggcgcgagtc gatcagctgt ccgcagaacg cacgcaacag tgtctgcgcc
     4801 actgtcgcca tgcggagctg cctcatcccc tggaatcgtg aagcagtctc gccgatcagc
     4861 ggatcgcgtg cgaagcgacg cagaaactcg gtgtggtcgg catcgacggc gaggacgaac
     4921 cgcaaccgct ccagaccttc ttcgctctcc gcgcgcagca cgaggcgtcc gtccagcgtc
     4981 tgcctggctg ttgcgacctc cgcgagctcg ccgacgcgca gcgtggctgt cagcgtcccg
     5041 tcgcggacac ggcgcgtcgc gtcgctcgcg tgcttcgcgc agagcgcgag cgtgtacggg
     5101 ccggcggggc gaacgacccg ctcgagggtc aactcttgcg ggttgcgagc tcggcgtcca
     5161 cgccgagatc gtacgcgcgg cggaggatag gctccgcctc ttcggctcgc ccttgctcct
     5221 gcaggagcga agcccagtcg agggaggcca ggcccgcgtt gtgccgccgg ccgtgcaccg
     5281 tcagaagatc gactgcgcgg cgatacgttt cgtttgcggc atccgtctcc ccctggagcg
     5341 caagcccacg ggcgagtgcc cagcaggccg cacccatctc gcccccgtgg aagttcccga
     5401 ggatcgcgag cgcgtccctg gcgcgatcga cggcgcgtgc actgtcaccc tcgagcgctg
     5461 ccaggcgaga ctggccgatg cgcagcatcc cgaggtccgc aggctccggg gtagggccga
     5521 ggagccgtgc gacgatctcg agttggttgc gggtctcgtc ggcgcgcccc tcctcggaat
     5581 cgatgccggc cgagaggagg tatccgcggg cgagtgtcag cgtgtcgtcg gttgcctcga
     5641 ggagggcgat cgccttgcgg atctgcgtga gcgcctcggc cgaacggcct tcgatcgcag
     5701 ccacacgagc gagcgaccag taaaggcgta cgcgcgtgta accgtcgcca gagtcgcccg
     5761 cccggctcaa cgcaccgcgg acgacctcgg tcgcgcggcc atagtcccct gcgtccgtca
     5821 gcgcatagct caggaacgtg gagtagcgga cgaggagcgt tacgtcgtcg ggcgttgccg
     5881 cggttacttc agcgaggcat tgttcgagca ggcgcgcagc gcggtccggt gctccgagcg
     5941 cagcgtagga ctgcgcgagc cccgtgtaca cgtcagggcg gaggtgggga gctacgcggt
     6001 catactcgag cgcggactcc agacgctcga ccgcgtcgag gtgattgccg cggtgggcag
     6061 cagcgagccc gagtccaatc gctgcacgac tcgctgagac ccgatcgcct gcagcgctcg
     6121 cgtcggcgag gacttcctgg agtttctcct ctgctgcgcc cgtgtccgaa tcgagtcgaa
     6181 gttgaagctc tgcgtccgcg agcttgagct cccggtcgtc gacctcacgg atgtcacggc
     6241 cggtctcgag gtactcgacc gaaacgccga gcttctgcgc cagcttgcgg agcgccttga
     6301 ccgacggcgt acgtgcaccc gcttcgatac gcgagatgta tgcatacgaa actccaggcg
     6361 atgagagatc gcgctgggag aagcctcgcg ccacgcgcag gcgcttgagg cgggctccga
     6421 cgccctctgt ggtctcgctc aaggacacgc tgctccggat cattaggagt tctcggacat
     6481 aagtcaaatt cttttctgga gtcactttct gtcaatcggc gcttcctcat gacaacataa
     6541 gcccctcgaa ctgtggatct ggttggggga aggagggacc atgcatccct ggggctccta
     6601 attgacattc ggcgggcggg agcgcccact acgctcccgc ccggtttact acccgcctga
     6661 tggcttcagg gctcgcggtc gctgtctacg ggctcctgct cttacccggc gccgtcctcg
     6721 tctggcggcg gcctgtcttc gccctgtacg cctggatcgt ggggctcgcc gcgcacaacg
     6781 ccgtgatggc ggccctgtac ggggccggcg tccgcggagg cgctctgacg gcgatccagg
     6841 cctggaagga gattctgctc ggcgtcgcgc tggcccgcgt cgcgctcgaa gccctgcgcg
     6901 ccggccggct tgccttccgg gttcagccgg cagacgtagt cgcggtcgcg ttcgcagcgg
     6961 tcgtctgcgt gtacgccgtg ctcccgcagg attggctcgg cgggacggcg gaccggtcgg
     7021 cgatcgggct cgcgctcaag cacgacctcg ttccggtggg cgcgtacttc ctcggccgct
     7081 ccgtcatcct gcgcagcgag gagctcgtcc gcctcgtctg gaccgtcgtc ggcgcggcgg
     7141 ctgcggtcgc cgtgctcggg ctcctagacg acttcttcat tccgatctcg tggtggcgcg
     7201 actccgcagt cgtggactac ttccacaagc acctcggcta cgactaccac ggcaccggcg
     7261 gcctcccgga gaacttcgtg ttcaacaacg gcggcgatgc ccacgggttc taccgccggc
     7321 tcgtgtcgac cttcctcggg ccgctcgcga gtgcctacat gttcgtcgtt gcgttgctgc
     7381 tcggcgcggc gttcctccgc cggccgcgca tcctcgtgcc gttgagcgcg gcgatcttcg
     7441 cgggcctgct cttcacgttc tcgcgttcgt cactccttgc actcgcggcg ggcctggtcg
     7501 tgctcgcttt cgtcacgcgg cgctggtggc cggtcgctgc cgccgtcgcg acgctcgccg
     7561 tcagcgtggc gtgggtgcac gtcttcccgc atgtcgcgcc gacgggcaag tggacgcagc
     7621 aggatctggt ggagcagcgg aaccacgcga aggcgcagcc tggagcgagc ggtagcgccg
     7681 ggagcatcga cgagccctcg attcacagcc actggatcag tctcagggaa ggtgtgcgca
     7741 ctgtcgtcca ccatccgcag gggtacggcc tcgccaacgc cgggcaaact gcatcgcgca
     7801 ccggtacgcc gatcaaagcg ggagagtcga actacacgga gatcggggcc gagacaggat
     7861 tgttcggagc gcttctctgg atcgcctggg ggctagcgat cttcgtcggt ctcgtcgccg
     7921 cggcgcggca cacacgctgg tgggcggcgt cagggatcgc ggccgcgttc gcagcggggc
     7981 tcgtgctcgc agtgcagacg gacgtgatcg gagatccctg gatggcttat tgcctctggg
     8041 cttgcgccgg catcgcgctc atgtcgctgc ctatcctccg agagtgagcg cgaccgcgca
     8101 aatccccgag acgcagcagc gcgtgcgtgt gatcttcggc gcgctgatcc tggtctttct
     8161 cctcgcgtcg ctcgaccaga cgatcgtgtc gacggcgctg ccgacgatcg tcggcgagct
     8221 cggcgggctt cagcacctct cctgggtggt caccgcttac ctgctcgcat cgaccgtgtc
     8281 gggaccgctc tacgggaagt tcggcgacct ctacggccgg aaggtcgtcc tgcagacggc
     8341 gatcgtcatc ttcctcgtcg gctctgctct gtgcgggctg agccagaaca tggcggagct
     8401 gatctccttc cgtgcgctgc agggtctcgg cgccggcggt ctcatcgtga cggcgatcgc
     8461 ggtcatcggc gacgtgattc cgccgcgcga ccgcggccgc taccagggga tcttcggcgg
     8521 tgtcttcggg gtttccacga tcatcgggcc gctgctcggc ggtttcttcg tcgacaatct
     8581 ctcgtggcgc tggatcttct acgtcaacct gccgatcgga gcggtcgcct tcgtcgtcat
     8641 cggcgccgtg ttccacgtgc gcccagcacc caaggagcac aaggtcgact acctcggtgc
     8701 ggcgctgctg gccggcggtc tctcggcaat cgtcctcttc accagcctgg gaggcacgac
     8761 gtgggcgtgg gattcgccgg agatcatcgc gctgatcgcg atcggtgtcg ttgcgattgg
     8821 cgcgttcgtg ctcgtcgagt cgcgcgtcgc tgagcccatc ctgccgctgt cgctcttccg
     8881 gaaccgcacc ttcgcagtga cgagcgcggt ggggttcgtc gtcgggctcg cgttgttcgg
     8941 tgcg
//
LOCUS       DASVEU010000076         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_252438,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000076 DASVEU010000000
VERSION     DASVEU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252438"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..378)
                     /locus_tag="VF225_02960"
     CDS             complement(193..378)
                     /locus_tag="VF225_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144242.1"
                     /translation="MNLLERIRSFWKPRPAADHPFTEQERDEQRPPTAYDERARTEEE
                     FIGDDLDPDEPRAGKLH"
     gene            complement(419..802)
                     /locus_tag="VF225_02965"
     CDS             complement(419..802)
                     /locus_tag="VF225_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144243.1"
                     /translation="MSGPFIFIATNRLKPGKLDDEKARVPGLVDFIEANEPQLIAFNE
                     YVNEEGTEVAVVQIHPDADSMAFHMEVVAERAAAAYAETVDATTGIQVFGTPSNAVVE
                     MLRRQAGAGVPLSVKPQHLGGFTRP"
     gene            824..1129
                     /locus_tag="VF225_02970"
     CDS             824..1129
                     /locus_tag="VF225_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7144244.1"
                     /translation="MPCGDGGCPRNHTVTSLARPSEVASPSAVTGASMNPARSFGPAL
                     VANEWHDFWVYLLGPPIGATVGAFAYQLVRGEHRDQSLIPSSPGAVTMLVVKLLKAA"
     gene            complement(1216..2160)
                     /locus_tag="VF225_02975"
     CDS             complement(1216..2160)
                     /locus_tag="VF225_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7144245.1"
                     /translation="MVAVIPFFNGAQVREAVSPERALEAVRAAFIAYARGEWTMPPKV
                     YVPAYPEGDFRAMPALGAGHALLKWVTSFPGNPAKGLPTVTGLVLLSDATNGMPKAVL
                     DAGAVTALRTGAAAVLAAETLGRADAETAAVIGAGVNGEAAAQTFIARGRRVQLWDID
                     RARAEAVAERTGAEVAPGRDEALAADLLVTVTPGRNILLEEGSLRPGQHASLMGADGP
                     GKTEIAVEELARVRVFCDDWEQASHNGDLAHAVEAGKITREDVTQLGDVLVGTAPGRQ
                     SNDDLTIFDSTGLAIQDLAIALAAIEQAEKLDLTTIEL"
     gene            complement(2148..>2718)
                     /locus_tag="VF225_02980"
     CDS             complement(2148..>2718)
                     /locus_tag="VF225_02980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7144246.1"
                     /translation="ALDAAAAGLGLAAASPILAAAAVAIKLDDGGPVFYRQRRVGLNG
                     DEFELVKLRTMQVGAERKGAGFAVNEGDPRITRAGRLLRRLSIDELPQLWNVVRGDMS
                     LVGPRPTLAYQVERYTPRQRRRLEVRPGITGWAQIQGRARLPWDERIELDVWYVEHRS
                     PWLDLKIIVRTPRALFAGTYKGDTGGWSR"
BASE COUNT          407 a          959 c          879 g          473 t
ORIGIN      
        1 ccgacgcacg accacgagag cggagcgccg ggcaggattc tcacctgagt tctctgtagt
       61 acaccgggcc cacaacaatt gaccggaagg tgcacgcacc ctgccgaatc atgcagaagt
      121 tggatctgaa gacccgcgcg gagctcaggc tctatgcgct cgccaagggg ctgatcgccc
      181 caactagccc ggtcagtgca gcttgcccgc ccggggctcg tccggatcga ggtcgtcgcc
      241 gatgaactcc tcctccgtgc gcgcccgttc gtcgtaggcg gtcggcgggc gctgctcatc
      301 gcgttcctgc tcggtgaacg ggtgatcggc ggcgggcctg ggtttccaga agcttcggat
      361 ccgctctagg aggttcatgt cgccatgttg tcagcttcgg gtggtaaccg ttccgtggct
      421 aaggacgcgt gaatccaccc aggtgctggg gcttgacgct caacggcacg ccggctcccg
      481 cttgccggcg caacatttcg acgacggcgt tgctcggcgt gccgaacacc tggatgcccg
      541 tcgtcgcgtc gaccgtctcc gcgtacgcgg ctgcggcgcg ctcggcaacg acctccatgt
      601 gaaacgccat cgagtcggcg tccggatgga tttggacgac cgcgacctcg gtgccctcct
      661 cgttgacgta ctcgttgaac gcgatcagct gtggctcgtt cgcctcgatg aaatcgacga
      721 ggcctggcac tctcgccttc tcgtcgtcga gcttccccgg cttgagccgg ttggtggcga
      781 tgaaaatgaa cggccccgac atgacgtctc cttccttccc cacatgcctt gtggggacgg
      841 tggttgccct cgtaatcaca ctgttacctc tctggcacga ccctccgaag tagcatcgcc
      901 gagtgccgtt accggcgcgt cgatgaaccc cgctcgctcg ttcggtcctg cgcttgtcgc
      961 gaacgagtgg catgacttct gggtctacct tctcggcccg ccaatcggag cgacggtcgg
     1021 cgcgttcgcg tatcagttgg ttcgcggcga gcaccgagac cagtccctca tcccgagcag
     1081 tccaggagcc gtgaccatgt tggtcgtcaa gctgctcaag gcagcctgag gcccgacctc
     1141 ccgcacgaag gacaggtcaa gcgctcgggc agtcgcgatc tcgagggacg gctcgccgca
     1201 gagcgttgaa ccgttctaga gctcgatcgt cgtcaggtcg agtttctcgg cctgctcaat
     1261 cgcggcgagg gcgatggcga gatcctgaat cgcgagcccg gttgagtcga agatcgtaag
     1321 gtcgtcgttg gactgacggc cgggagccgt gcccacaagg acgtcgccca gctgggtcac
     1381 gtcctcgcgc gtgatcttgc cggcctcgac cgcgtgggcg aggtcgccgt tgtgactggc
     1441 ttgctcccag tcgtcgcaga agacacgcac acgagccagc tcttcgacgg cgatctccgt
     1501 cttgcccggg ccgtctgctc ccatcaggct tgcgtgctgg ccgggacgaa gtgagccttc
     1561 ctccagcagg atgttgcggc cgggtgtgac cgtcactagt agatcggccg cgagagcttc
     1621 gtcccgaccc ggcgcgactt ccgctccggt ccgctccgcc accgcctcgg cccgggcgcg
     1681 gtcgatgtcc cacagctgca cgcggcggcc acgcgcgatg aaggtttggg ccgcggcttc
     1741 gccgttcacg ccggccccga tgaccgcggc ggtctcggca tcggctcggc ctagtgtctc
     1801 cgccgccagc accgcagccg cgcccgtgcg cagagcggtc accgcacccg cgtcgagcac
     1861 cgccttcggc atcccgttcg tggcgtcgga gagcagcacg agcccggtga cggtcggcag
     1921 ccccttggcg ggattgccgg ggaacgacgt cacccacttc agcagcgcgt gacccgcgcc
     1981 caacgcgggc atcgcgcgaa agtcgccctc cggataggcg ggcacgtaga ccttcggcgg
     2041 catcgtccac tcgccgcgcg catacgcgat gaacgccgcg cgcaccgcct cgagcgcacg
     2101 ctcgggcgag actgcctcgc gcacctgcgc cccgttgaag aacgggatca ccgcgaccac
     2161 cctcccgtat cgcccttgta cgtccccgcg aagagcgcac gtggcgtgcg cacgatgatc
     2221 ttcaagtcga gccacgggga gcgatgttcg acgtaccaga cgtccagctc gatccgctcg
     2281 tcccacggca ggcgtgcccg gccctggatc tgcgcccagc cggtgatgcc gggtctcacc
     2341 tcgagtcggc gccgctgccg cggcgtatag cgctcgacct ggtacgcgag cgtggggcgg
     2401 gggccgacga ggctcatgtc accgcgaacg acgttccaga gttgcggcag ctcgtcgatc
     2461 gagagccgcc gcagcagtcg accggcgcgc gtaatgcgcg gatcgccctc gttgaccgcg
     2521 aagcctgcac ccttccgctc cgccccgacc tgcatcgttc tcagcttcac gagctcgaac
     2581 tcgtcgccgt tcaggcccac tcggcgctgc cggtagaaga caggcccgcc gtcgtccagc
     2641 ttgatcgcga ccgctgccgc cgcaaggatc gggctcgccg cggccagccc gagcccggcg
     2701 gctgcagcgt cgagcgcg
//
LOCUS       DASVEU010000077         7153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_57758,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000077 DASVEU010000000
VERSION     DASVEU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57758"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF225_02985"
     CDS             <1..347
                     /locus_tag="VF225_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144247.1"
                     /translation="VAFYRLLGLEFPEGAEDEEHVEAPLPGGMRYTLDTEDVMRGFDP
                     GWKRPSDGHAVGGAFRCDSPDEVDQVHRDLLAAGATEHKEPWDAFWGQRYAQVKDPDG
                     TVIDLYAPLPDS"
     gene            complement(344..1423)
                     /locus_tag="VF225_02990"
     CDS             complement(344..1423)
                     /locus_tag="VF225_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7144248.1"
                     /translation="MTFEEARALFPVLERFAYLNTGTLGPLSRPTLAAVEERLHFDQE
                     RGRGGHGWFMTVLELRARVREQLAAQVGASPGQMALTSSTTDGCNIVLSGLELGPDDE
                     VVTTDSEHPGLLLPLHVSGVRVGVAKVAGRPTEDALSSILACITPRTRLLALSHVLWT
                     TGQVMPVHDLKRESALPMLVDGAQSVGAIPVDVGELDYYTVSCQKWLCGPEPLGALYV
                     RDPDQLAVAIPSGFAQKSLEPDGTFVPKEGAARFDSGWLATPSLAGLEAALAGSPEWR
                     FERAAEMAGLCRGAMRDRFELVADGGESTLVSFVPRGEAAAEAARLYEAGVIVRDLPG
                     TGWLRASCGWWTGGDDIDRLVAALG"
     gene            complement(1420..2259)
                     /locus_tag="VF225_02995"
     CDS             complement(1420..2259)
                     /locus_tag="VF225_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7144249.1"
                     /translation="MTTRIGSTVERLLVRTWNLFHGNTQPPGRKAFLREMVELASADR
                     PTLLCLQELPVWTLDLLDDWSGMTAVGAVAQRPTLGPFPSTPELGRALTEVHHGRLRS
                     AFTGQANAILLSSDLQLREHRHLILNPFGFRHAQARRLGLGIIERLAWGKERRVCHAV
                     RAERGDETLVIGNLHATGFADKGVPDAELLRAAVFVDGMAKRGEPVLLCGDFNVGVGN
                     SRTLTDLTRVDWGFSGPTATGIDHILVRGLEASPPVRWPGERRRHEGRLLSDHAPVER
                     TVA"
     gene            2246..3292
                     /locus_tag="VF225_03000"
     CDS             2246..3292
                     /locus_tag="VF225_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7144250.1"
                     /translation="MRVVISDPPAFTPAYDHELAAALARRAVDVELVTSRFRFGETPK
                     PVGYRRSEPFYPISSRLFGRSRLRIPLKVMEHMPGMAVLLLQRADVLHMQWLAAPELD
                     RLLLRPRVPSVFTAHDLLPRRTARKLGLWKDLLARFDRVVVHSERGRESLAELGVDAA
                     VIPHPVFPSDPERADDGHTLLCLGTIRPYKGIGDAIAATKQIPGARLLVAGDPVESVN
                     GYRAEAGELAEWRLGYLSQPELERALGDATVALFPYRPELDQSGALLQALGAGVPAVA
                     YDIGGLAEPIEKFGAGRVVAAGDVEALAGAARELLEDDAALARARAGARKARDTLTWD
                     AAAAAHIRLYEGLL"
     gene            3289..3603
                     /locus_tag="VF225_03005"
     CDS             3289..3603
                     /locus_tag="VF225_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144251.1"
                     /translation="MRVRRARFSELIDRQLDLFEREQRGLIEDCIAAEGAYNRADRDE
                     AEERYGDYVDLVETGTEILADLRDNFATTLEEDVAEEYERAFNQTVLRRFRRFALEIE
                     DR"
     gene            3603..5423
                     /locus_tag="VF225_03010"
     CDS             3603..5423
                     /locus_tag="VF225_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7144252.1"
                     /translation="MARIEDYGMIGDLQTAALVGRDGSIDWACFPRFDSGACFAALLG
                     TPEHGRWLVAPRADAWEGERRYRPCTLVLETEWETDTGAVRVIDFMPPRGKAPDIVRI
                     VEGLRGHVEMFSELVIRYDYGGTVPWVRRTADGRLAVAGPDALCFRTPVEHRGENLRT
                     LGEFTVRKGDRVPLTLTWYPSNERPPRAIDAEHALNETVAFWQEWADRCGYEGKWKED
                     VQASLVVLKGLTYAPTGGMVAAPTTSLPEKIGGERNWDYRYCWLRDATLTLLAFLNAG
                     YLEEARAWRVWLLRAAAGDPSALQIMYGVAGERRLFELTLDWLPGYEGSTPVRVGNAA
                     AGQFQLDVYGEVLDALHQGRVHKLEVSKEAWALQRRLLGFLEDAWKEPDEGIWEVRGP
                     RRHFTHSKVMAWVAFDRGVQAIERFGRNGPVERWRAIRTEIHREVCEQGYDPELNSFT
                     QFYGSKRLDASLLMIPLVGFLPADDARMVGTVAAIERELVHDRLVYRYIPDEEAQSVD
                     GLPPGEGAFLPCTFWLADNLALQGRLDEAEEIFERLLDLRSDLGLLAEEWDPNARRQL
                     GNYPQAFTHVALVNTAFNLSQQQRGSPMEQRGPYEEPAAY"
     gene            5498..6628
                     /locus_tag="VF225_03015"
     CDS             5498..6628
                     /locus_tag="VF225_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144253.1"
                     /translation="MLRRTVVLAVALTALATVLVVSAQGSGSGSDRYIVVLKNAVDTS
                     AVANLHAVRYGTSVENVWSSALHGYSAVIPNDRVAALRADENVAYIELDGIATATAQT
                     LPWGVDKVDADVSSTLAGNGSGAITNVDAYVIDTGIDASHPDLNVVEFKQYANGPPRD
                     CNGHGTHVAGTIGARDDTAGVVGVAPGIRLHAIKVLNCAGSGSWSDVISGINYVAGTS
                     TRPAVANMSLGGPQNTAVDDAVKGAVAKGVFFGVAAGNDGGNACGHSPAAAGTTSGVD
                     TVAATDSSDAEASWSNYGNCVDIWAPGVSIYSTYKSGGYATLSGTSMATPHVVGGAAL
                     YLSSHPTAGPGTVETALRNAATTTTKTSKDGRTIIREYVGGF"
     gene            complement(6635..>7153)
                     /locus_tag="VF225_03020"
     CDS             complement(6635..>7153)
                     /locus_tag="VF225_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144254.1"
                     /translation="GGEEKQLALPLDPTAATPELPEPTVWERMLADYRTTNLSVGVHP
                     LELLRAHLPAGVISSRDLEQASDRAQVSIAGMAVARQRPATANGVVFMLIEDEFGAVN
                     LIVPPSVYDKHRAIVRGEPLILARGRFERIERNQNVLVHSFETLGPLARQVSQETEVG
                     ASLPGAHHFGHR"
BASE COUNT         1200 a         2433 c         2406 g         1114 t
ORIGIN      
        1 cggtggcgtt ctaccgcctg ctcgggctcg agtttccgga aggcgcggag gacgaggagc
       61 atgtcgaagc gccgcttccg ggcggcatgc gctacacgct cgacacggag gacgtcatgc
      121 gggggttcga tcccggctgg aagcggccgt cggacggtca cgccgtgggc ggagccttcc
      181 ggtgcgactc gccggacgag gtcgaccagg ttcaccgcga tctcctcgcc gcaggcgcga
      241 cagagcacaa ggaaccctgg gacgcgttct ggggtcagcg ctacgcgcag gtcaaggacc
      301 ctgacgggac tgtgatcgac ttgtacgccc cactgcccga cagctaaccg agcgcagcca
      361 cgagccggtc gatgtcgtcg ccgcccgtcc accaaccaca cgacgcgcgt agccagccgg
      421 tgccgggcag gtcgcgcacg atcacgccgg cctcgtagag ccgcgcagcc tccgccgcgg
      481 cttcgccccg ggggacgaac gagacgagcg tgctctcccc gccgtccgcc acgagctcga
      541 agcggtcacg catggcgccg cgacagaggc ccgccatctc ggcggcgcgc tcgaaacgcc
      601 actcgggtga gccggcgagc gccgcttcga ggcccgcgag gctgggcgtc gccagccagc
      661 cggagtcgaa tcgcgctgcg ccttctttgg gcacgaaggt tccgtccggc tcgagggact
      721 tctgcgcaaa cccactcggg atcgcgacgg cgagctggtc cgggtcgcgg acgtagagcg
      781 cgcccagggg ctccggcccg caaagccact tctggcacga cacggtgtag tagtcgagct
      841 cgccgacgtc gaccggaatc gcaccgactg actgggcacc gtccaccagc atgggcaggg
      901 cgctctcccg tttcaggtcg tgcaccggca tcacctggcc ggtggtccac aggacgtgcg
      961 acagggcgag cagtcgtgtc cgcggagtga tgcacgccag gatcgacgag agcgcatcct
     1021 cggtcggccg ccccgccacc ttggcgaccc ccactcgaac cccagagacg tgaaggggca
     1081 acagcaaccc cggatgctct gagtccgtcg tgaccacctc gtcgtccggc ccgagctcca
     1141 gaccggacag cacgatgttg cagccgtcgg tcgtggagct cgtcagcgcc atctggccag
     1201 gggacgcccc gacctgtgcg gcaagctgct cgcgcacgcg ggcccgcaac tcgagcaccg
     1261 tcatgaacca gccgtgacct cctcgcccgc gctcctggtc gaagtggagg cgttcctcca
     1321 ccgccgcgag cgttgggcgg gacaaagggc ccagcgttcc cgtgttcaga tatgcgaacc
     1381 gctcgagaac ggggaacagc gcgcgagcct cctcgaacgt caagcgaccg tccgttcgac
     1441 cggtgcgtga tcggagagca aacgcccctc gtgccggcgc cgctctccag gccagcggac
     1501 gggcggcgaa gcctcgaggc cccggacgag gatgtggtcg atgccagtcg ccgtcgggcc
     1561 ggaaaatccc cagtcgactc tggtcagatc cgtgagcgtc cgcgagttgc cgacgccgac
     1621 gttgaagtcg ccgcagagaa gaacaggctc accgcgcttg gccatcccgt cgacgaacac
     1681 cgctgcgcgc aggagctcgg cgtccggcac gcccttgtcc gcaaagccgg tcgcatggag
     1741 gttcccgatg accagcgtct cgtctccgcg ctcggcccgc acggcgtgac acacacgtcg
     1801 ctccttaccc cacgccagcc gttcgatgat gccgagtcca agccgccgcg cctgcgcgtg
     1861 ccggaagccg aaggggttga ggatcaagtg acgatgttct cggagttgca gatccgagga
     1921 gagcaggatc gcgttcgcct gtcctgtgaa cgccgagcgc agccttccgt ggtgaacctc
     1981 cgtcagcgcc cgcccgagct cgggtgtcga gggaaacggc ccgagcgtcg gacgttgtgc
     2041 aaccgccccc accgcagtca tcccgctcca gtcgtccagt aggtcgagcg tccagacagg
     2101 cagttcttgg aggcacagca gggtcggacg atcggcgctc gcaagctcca ccatctccct
     2161 gagaaacgcc ttacgccccg gcggctgtgt gttcccgtga aagaggttcc aggtccggac
     2221 aagcaggcgc tctactgtac tgccgatgcg agtcgtcatc tcggaccccc cagccttcac
     2281 accggcatac gaccacgagc tcgcagcggc actagcccgc cgcgcagtcg acgtcgagct
     2341 cgtcacgtct cggttccgct tcggcgagac gccaaaacct gtcggctacc ggcgcagcga
     2401 acccttctat ccgatctcgt cgaggctgtt cggacgctcc cgcttgcgca tcccgctgaa
     2461 ggtcatggag cacatgcccg gcatggcggt gctgctgctt cagcgagcgg acgtcttgca
     2521 catgcaatgg ctcgccgcgc ccgagctcga tcggctgttg ctgcggcctc gcgtgccgtc
     2581 tgtcttcacc gcgcacgatc tgctgccgcg acgaacggcc cgaaagctcg ggctctggaa
     2641 agacctgctc gctcgcttcg accgcgtcgt cgtccacagc gagcgcggac gcgagagtct
     2701 tgccgagctc ggtgtcgacg cggcggtcat ccctcatccc gtcttcccga gtgaccccga
     2761 acgtgccgac gacgggcaca cgttgctctg tctcggcacg atccgaccgt acaaagggat
     2821 cggagacgcg atcgccgcga cgaaacagat ccccggtgcg cgcttgctcg ttgccggcga
     2881 cccggtcgag tcggtgaacg gttaccgggc ggaggcgggc gagctagccg aatggcggct
     2941 cggctacctg tcacagcccg agctcgagcg tgcgctcggc gacgcgacgg tcgcactctt
     3001 tccctaccgg ccagagctcg accagagcgg tgcgctcctg caggcgctcg gcgccggagt
     3061 gcccgcggtg gcgtacgaca tcggtgggct tgcggagccg atcgagaagt tcggtgcggg
     3121 acgtgtcgtg gcggccggtg acgtcgaggc gctcgccggc gccgcccgtg agctgctcga
     3181 ggacgacgcg gcgctcgcgc gtgcccgagc aggggcgcgc aaggcgcgag acacgctgac
     3241 gtgggacgcg gccgccgccg cgcacatccg gctctacgag ggattgctat gagggtcagg
     3301 cgcgcccgct tctctgagct catcgaccgt cagctcgacc tcttcgagcg cgagcagcgc
     3361 gggctcatcg aagactgcat cgcagccgag ggcgcgtaca accgggcaga ccgcgacgag
     3421 gcggaggagc gctacggcga ctacgtcgac ctcgtcgaga ccggcacgga gattctcgcc
     3481 gacctccgcg acaacttcgc cacgacgctc gaggaggacg tggccgagga gtacgagcga
     3541 gccttcaacc agaccgttct gcgacgcttt aggcgtttcg cactcgagat cgaggaccgc
     3601 tgatggcacg aatcgaggac tacggaatga tcggcgacct ccagaccgcg gcgctcgtgg
     3661 ggcgcgacgg ctcgatcgac tgggcatgct tcccgcgctt cgactcgggc gcctgcttcg
     3721 cagcgctact cggcacgccg gaacatggac gctggctcgt cgcaccgcgg gcggacgcgt
     3781 gggagggcga gcgccggtat cgcccgtgca cgctcgtgct ggagacggag tgggaaacgg
     3841 acaccggcgc cgtacgcgtc atcgacttca tgccgcctcg agggaaggcg ccggacattg
     3901 tgcgcatcgt cgaagggttg cgcggtcacg tcgagatgtt ctccgaactc gtcatccgct
     3961 acgactacgg cggcacggtt ccatgggtca ggcggaccgc ggacggtcgc ctcgcagttg
     4021 ccgggccaga cgcgctctgc ttccgcactc ccgtcgaaca ccgcggcgag aacctccgca
     4081 ccctcggcga gttcactgtg agaaagggcg accgcgtccc cttgacgttg acctggtacc
     4141 cgtcgaacga acggccaccg cgagcgatcg acgcagagca tgctctgaac gaaacggtcg
     4201 ctttctggca ggagtgggcc gaccgctgtg gctatgaggg caaatggaag gaggacgtcc
     4261 aagcgtcact cgtcgtcctg aaaggcctca cctatgcccc gaccggcggc atggtggcgg
     4321 cgccgacgac gtcgctgccg gagaagatcg gcggggagcg gaactgggac taccgctact
     4381 gctggctccg agatgcgacg ctgaccttgc tggcattcct caatgcgggc tatctcgaag
     4441 aggcccgcgc ctggcgtgtc tggctgctgc gtgccgctgc cggcgacccg tcggcgctgc
     4501 agatcatgta cggcgtcgcg ggcgagcggc ggctgttcga gctcacgctc gactggctgc
     4561 ccggctacga gggctcgacg cccgtgcgcg tgggcaacgc ggcggccggg cagttccagc
     4621 tcgacgtgta cggagaggtg ctcgacgcgc tccatcaggg tcgtgtccac aaactggagg
     4681 tgtcgaagga ggcctgggcg ctccagcgcc ggctgctcgg cttcctcgag gacgcctgga
     4741 aggagcccga cgaaggcatc tgggaggtgc gcggcccgcg ccggcacttc acgcactcca
     4801 aggtgatggc ctgggttgcc ttcgaccggg gcgtccaggc cattgagcgc ttcggccgca
     4861 acggccccgt cgagcggtgg cgcgcgatcc gcaccgagat ccaccgagag gtctgcgagc
     4921 agggttacga tccggagctc aactccttca cgcagttcta cggctcgaag cggctggacg
     4981 cgagcctgct catgatcccg ctcgtcggtt ttctccccgc tgacgatgca cggatggttg
     5041 gcactgtcgc cgcgatcgag cgtgagctcg tccatgaccg attggtctac cgctacatac
     5101 ccgacgagga agcccaaagt gtcgacggct tgccacccgg cgagggcgcg ttcctgccgt
     5161 gcaccttctg gctcgccgac aatctcgccc tgcagggccg gctcgacgag gccgaggaga
     5221 tcttcgagcg gctgctcgac ctgcggagcg acctcgggct cctcgccgag gaatgggacc
     5281 cgaacgcgcg gcgccagctt ggcaactatc cgcaggcgtt cacgcatgtc gcgctggtga
     5341 acacagcgtt caatctcagt caacagcagc ggggttctcc aatggagcag cgcggaccgt
     5401 acgaggaacc ggcggcctac tagagcacga ccttgtatcc gtcgtcacgt cgctctacca
     5461 ttcgaccggc cgttagtcat tctctttgga ggggtccatg ctcagacgca ctgttgtgct
     5521 cgccgtcgct ctgactgcgt tggcaactgt tctcgtagtg tcggcccagg gaagtggatc
     5581 tgggtcagat cggtacatcg tcgtcctcaa gaacgcggtc gacaccagcg ccgtcgcgaa
     5641 tctccacgcg gtgcgttacg gcacaagcgt ggagaacgtc tggtcctccg cgctgcacgg
     5701 ctattccgca gtgattccaa acgatcgagt cgccgccttg cgggccgacg agaacgtggc
     5761 atacatcgag ctcgacggga tcgcgaccgc aacagcgcaa acgcttccgt ggggcgtcga
     5821 caaggtcgat gccgacgtca gctccacgct cgccggtaac ggttcaggtg cgatcacgaa
     5881 cgtcgacgcg tacgtcatcg acaccgggat cgacgcctcg catcctgatc tcaatgtggt
     5941 cgaattcaag caatatgcca acggcccgcc acgcgattgc aacgggcacg gcacgcatgt
     6001 cgcgggaacg atcggtgctc gcgacgacac cgccggcgtc gtcggggtcg cgccgggcat
     6061 tcgcctccac gcgatcaagg tgctgaactg cgcggggagc ggcagctggt ccgacgtcat
     6121 ctccggcatc aactacgtcg ccggcacgag cacgcggccg gcggttgcga acatgagcct
     6181 cggcgggccg cagaacaccg cggttgacga cgccgtgaag ggcgccgtcg ccaagggcgt
     6241 gttcttcgga gtggctgcag gaaacgacgg tgggaacgcc tgcgggcact cacccgcagc
     6301 agcgggaacc acgagcggcg tcgacaccgt tgccgccacc gacagcagcg atgccgaggc
     6361 ttcgtggagc aactacggca actgcgtcga catctgggct cccggcgtca gcatctactc
     6421 gacgtacaag agtggcggct atgcgacgct ctcggggacg tcgatggcga cgccgcatgt
     6481 cgtaggtggc gcagctctct acctctcctc gcatccgacc gcgggtccgg gaacggtcga
     6541 gactgctctt cgcaacgctg cgactacgac gaccaagaca agcaaggacg gccgcacgat
     6601 cattcgcgag tacgtcggcg ggttctagct ccgactaccg atgaccgaaa tggtgggcgc
     6661 cgggcaggct ggcgcccacc tcggtctcct gcgagacctg gcgcgctagc gggccgaggg
     6721 tctcgaacga gtggacgagc acgttctggt tgcgctcgat ccgttcgaag cggccgcgcg
     6781 cgaggatgag cggctcgccg cgcacgatcg cccgatgctt gtcgtagacg gagggcggca
     6841 cgatcaggtt caccgcgccg aactcgtcct cgatcagcat gaagacgaca ccgttcgcgg
     6901 tcgccggccg ctgccgcgcg acggccatcc ccgcaatcga cacctgcgca cgatccgatg
     6961 cctgttcgag gtcacgcgac gagatgacgc ccgccggcag atgtgcgcgc aagagctcca
     7021 acggatggac gccgaccgag aggttcgtcg tgcggtagtc ggcgagcatg cgttcccaga
     7081 cggtcggctc cggcagctcg ggtgtcgccg cagtgggatc gagcggaagc gcgagctgct
     7141 tctcctcacc gcc
//
LOCUS       DASVEU010000078         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_253532,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000078 DASVEU010000000
VERSION     DASVEU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253532"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="guaA"
                     /locus_tag="VF225_03025"
     CDS             <1..1263
                     /gene="guaA"
                     /locus_tag="VF225_03025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7144255.1"
                     /translation="GGTVERTGASEFGKTDLTAVESELFAGLPEEETVWMSHRDTVTA
                     PPSGARVVATSRSTPVAAFEDAARRLYGVQFHPEVVHTPHGTELLKNFLYNVAGAPPA
                     WTPAAVIEEQVERIRAAVGRERVLCALSGGVDSAVAALLVHKAIGDQLTCVFVDHGLL
                     RENEAEQVVETFDGHFRVPLVHVQAQERFLSRLEGVDDPERKRKIVGEEFIRVFEEEA
                     GRLGDIRYLVQGTLYSDVIESGGGEDGVAAMIKSHHNVGGLPADMRMDLVEPLRLLFK
                     DEVRRVGEELGMPERMVWRQPFPGPGLAIRIIGDVTEERLTILRSADAILQEEIRRAG
                     LYRELWQSFAVLPAIRSVGVQGDERTYAYPIVIRAVTSDDAMTADWARLPYDLLETIS
                     SRIINEIPGVNRVALDLSSKPPATIEWE"
     gene            complement(1344..1610)
                     /locus_tag="VF225_03030"
     CDS             complement(1344..1610)
                     /locus_tag="VF225_03030"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7144256.1"
                     /translation="MIRRRVVVHGLVQGVFFRDSTRRLAQRHGVAGWVANRPDGAVEA
                     AFEGEVDAVERLVAFSREGPRGAQVESVEVIEEDPEGLCGFAVR"
     gene            1630..2232
                     /locus_tag="VF225_03035"
     CDS             1630..2232
                     /locus_tag="VF225_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7144257.1"
                     /translation="MDLREILRRRRMVRAYEPEPIPRETIERIVETVRRAPSAGFSQG
                     QRLVVVTDADTRQRIADLLEEGSGATLESWLRAPVLIVVGVREDDYHDRYRKPDKLSD
                     GAEIEWPIPYWHFDAGAAAMLILLAAIDEGYAAGLFGVFAEAMQPFKELLRIPDDVAV
                     ACCITIGKPADDSQWSAISSRLTQARKSVDDLVHWERWGY"
     gene            complement(2229..>3049)
                     /locus_tag="VF225_03040"
     CDS             complement(2229..>3049)
                     /locus_tag="VF225_03040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7144258.1"
                     /translation="RLVLPQARGIGDNGSLPRVAPDGTVWLATSSFGNSNGPYTMSLT
                     SSRNGGKSWARRRVIVRHTVNGYRNTTFRAAFGEAFTVGPRKVGRFYPLYAAYEQSSG
                     SETTLFIRGSFDGGKRWSRAIQVNDAPGPGDALQPNLAVAPTGTVAVAFYDRRLACPE
                     SDTPEATAAGLLFDPRTPQGRQNYCINTAIQFYRPKLRPLGHNIRLSEHTWDPQLSSP
                     RFDCICNPASFIGDYFGIDSRGGFTYTASVETFNAAGENPGYHQQQLVSKVPTP"
BASE COUNT          509 a          975 c         1073 g          492 t
ORIGIN      
        1 ggcggcaccg tcgagcgaac cggcgcgtcc gaattcggca agaccgatct gaccgccgtc
       61 gagtcggagc tcttcgcggg cctgccggag gaggagaccg tttggatgtc gcatcgcgac
      121 accgtcacag cccctccatc cggagcgcga gtcgtggcaa cctcgcggtc gacacccgtg
      181 gcggcgttcg aggatgcggc acggcggctg tacggggtgc agttccatcc ggaggtcgtc
      241 cacacgccac acggcaccga gctgctgaag aacttcctct acaacgtcgc gggcgcgccg
      301 cccgcttgga cgccggcggc agtgatcgag gagcaggtgg agcggatccg cgcggccgtc
      361 gggcgcgagc gcgtcctctg cgctctgtcc ggcggcgtcg attcggccgt tgccgcgttg
      421 ctcgtgcaca aggcaatcgg cgatcagctc acctgtgtgt tcgtcgacca cggcctgctc
      481 cgcgagaacg aggccgagca ggtggtggag accttcgacg gacacttccg tgtgccgctc
      541 gtccacgtgc aggcgcagga gcgcttcctg tcacgactcg agggcgtcga cgatccggag
      601 cggaagcgga agatcgtcgg cgaggagttc atccgggtct tcgaggagga ggccggcaga
      661 ctcggagaca ttcgctatct ggtccagggg acgctctact ccgacgtgat cgagtcgggc
      721 gggggagaag acggagtcgc cgcgatgatc aagtcccacc acaacgtggg cgggctgccc
      781 gcggacatgc ggatggacct cgtcgagcct ttgcggctgc tcttcaagga cgaggtgcgc
      841 cgggtgggtg aggaactggg catgccggag cggatggtgt ggcgccagcc gttccccggc
      901 ccggggctcg cgatccggat catcggcgac gtcaccgagg agcggctgac gatcctgcgg
      961 tcggccgatg cgatcctgca ggaggagatc cgccgggcgg ggctgtatcg cgagctgtgg
     1021 cagtcgttcg ccgtgctgcc ggcgatccgc tcggtcggcg tccaaggcga cgagcgcacg
     1081 tacgcgtatc cgatcgtcat ccgcgccgtt acatcggatg atgcgatgac agcggactgg
     1141 gcgcggctgc catacgacct gctggagacg atctcgagca ggatcatcaa cgagatcccg
     1201 ggcgtgaatc gagtcgccct cgacctgagc tcgaaacctc cggccacgat cgagtgggag
     1261 tagcgggctc gtcgtccgcg tggctcctcg ggacacgtac cggtgccagg cacgggtacg
     1321 aaactgtgac gactgtgagc cggctaacgc acggcgaaac cgcacaggcc ttccgggtct
     1381 tcctcgatca cctcgactga ctcgacctga gcgcctcgcg ggccttcgcg gctgaaagcg
     1441 acaagccgct cgaccgcatc cacttcgcct tcgaaggccg cctcgactgc tccgtccgga
     1501 cggttcgcca cccagcccgc aaccccgtgc cgctgcgcga gccgccgggt cgagtcccgg
     1561 aaaaacacgc cctgcacgag gccgtggacg acgacacggc gccggatcac ttgtcgtatt
     1621 ctcgccgaga tggatctgag ggagatcctg aggcgccgcc ggatggtgcg cgcctacgaa
     1681 ccggagccga tcccccggga gacgatcgag cggatcgtcg aaaccgttcg gcgtgcgccg
     1741 agtgccggtt tcagccaggg acagcggctc gttgtcgtaa cggacgccga cacacggcag
     1801 cggatcgccg acctcctcga agaggggtca ggtgcgacgc tcgagtcgtg gttgagggcg
     1861 cccgtgctca tcgtcgtggg agttcgcgag gacgactacc acgaccgcta ccgcaagccg
     1921 gacaagctca gcgacggcgc ggagatcgaa tggcccatcc cgtactggca cttcgatgcg
     1981 ggtgccgcgg ccatgctgat tctgctcgca gcgatcgacg agggctacgc cgccgggctg
     2041 ttcggggtgt tcgcggaagc aatgcagcca ttcaaggaat tgctgcgcat cccagatgat
     2101 gtggccgtgg cctgttgcat caccatcggc aagcctgcgg acgattcgca gtggagcgcg
     2161 atctcgagca ggttgacgca ggccaggaaa agcgtggacg acctcgtcca ctgggagcgc
     2221 tggggctact agggcgtcgg gaccttcgag accagttgct gctggtggta gcccgggttc
     2281 tcgcccgcgg cgttgaacgt ctcgaccgac gcggtgtagg tgaacccgcc gcgtgagtcg
     2341 atgccgaaat agtcgccgat gaagctcgcc gggttgcaga tgcagtcgaa gcgcggtgac
     2401 gacagctgtg gatcccacgt gtgctcggaa agacggatgt tgtggccgag cggtcgaagc
     2461 ttcgggcgat agaactggat tgcggtattg atgcagtagt tctgccgccc ctgtggcgtg
     2521 cgcgggtcga agagcagtcc cgcagcggtc gcctcgggcg tgtccgactc cggacacgcg
     2581 agtcgccggt cgtagaaagc cactgcgacg gtcccggtgg gagcaaccgc gagatttggc
     2641 tgcaacgcgt caccggggcc gggtgcatcg ttcacctgga tcgcccgcga ccagcgcttg
     2701 ccgccgtcga acgaaccgcg gatgaacagc gtcgtctcgc tgccgctcga ctgctcgtac
     2761 gcggcgtaca acgggtagaa gcggccgacc ttccgtgggc cgactgtgaa cgcctccccg
     2821 aaagcggcgc ggaacgtcgt gttccggtag ccgttgacgg tatggcggac gatcacccga
     2881 cggcgcgccc acgatttgcc gccgttccgc gacgaggtca acgacatcgt gtaggggccg
     2941 ttcgagttgc cgaagctcga cgtcgccagc cagacggtcc cgtccggcgc gacgcgtggc
     3001 agcgagccgt tgtccccgat ccccctggcc tgcggcagca cgagccgcg
//
LOCUS       DASVEU010000079         9684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1418153,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000079 DASVEU010000000
VERSION     DASVEU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418153"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF225_03045"
     CDS             <1..311
                     /locus_tag="VF225_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144259.1"
                     /translation="RAAADKAAEPGAQTAVYAIEQLRSWDWLMWTPGLTVMMVAAGLG
                     GLRTRALPKPLSWTSVVLGVAFFTPLGFFAFFILPPWMIATGVVLYRRQRVAAAGVPA
                     "
     gene            complement(314..1693)
                     /locus_tag="VF225_03050"
     CDS             complement(314..1693)
                     /locus_tag="VF225_03050"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX7144260.1"
                     /translation="MRSRAGSQTKFLLEESQLPRHWYNIRSDMPTALQPVLHPGTGQP
                     VGPDDLAPLFPMELIGQEVSEEAEIAIPEEVLDIYSLWRPTPLYRASRLEAAIGTRSR
                     IFYKYEGVSPPGSHKPNTAVAQAFYNKKEGRTRLATETGAGQWGSALSFACKLIGLEC
                     KVYMVRVSYEQKPARRSMIKAWGAEIVPSPSDETQSGRAILEEHPDSPGSLGIAISEA
                     VEDAAGRDDTAYSLGSVLNHVLLHQTVIGQEALAQMELAGAFPDVVIGCVGGGSNFAG
                     LAFPFLREKIAGREIDVLACEPAACPTLTRGLFAYDFGDTSKLTPLVPMHTLGHDFVP
                     APIHAGGLRYHGVAPLISQLLRDGLIRAEAYLQNDVFASAVLFAGNEGILPAPEAAHA
                     IHGTLQVAKAADEAGEERTILFNLSGHGHFDMAAYDNYFAGKLEDVALDEDEIERALR
                     AIEGLPVPA"
     gene            complement(1674..3182)
                     /locus_tag="VF225_03055"
     CDS             complement(1674..3182)
                     /locus_tag="VF225_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7144261.1"
                     /translation="MLRRVLGLSLLSLVVVSGAGAKPRPQAKAETPSDPVAWTSYGYD
                     NQLRNAVPSTLLTLRTVPGLAPAWKAGLDGPVYASPLSARVDGEQVAYVATEAGSVYA
                     LAVTTGAVLWQRELGTTTTELCGTWGITSTGAIDLARGLMYVIGATGQLHALSLATGE
                     EATGYPRQVVSRPDIEYVWGGLRIARDRLYVPIASYCDVGSTDGFPEGSLLAISLDAA
                     RASTTWDPVPGPDNLGGMWGWGGVSIAPTTGVVYTGVGNSHVWSDECSCFVDNAGYGD
                     RIVALSPDLTRVLDSSQLPDIPPTGDDDFGAAALLFQPETCPPLAAANNKNGTLYILD
                     REHLSRGSIVFIPLGDGVAPFIGEPAWSVEKQMIYSAQSVLYGDDGKRLGNGVRAFHV
                     DAGCGFRPIWSRALGDGNQAKPLIVGDVLFATGGTPGGFYALDAANGVSLWSYATTGG
                     TVAAMITVAGTVLGADTEGTVYAFRPAQPARGCTGRAEVLCSRRAADAESRR"
     gene            complement(3243..4688)
                     /locus_tag="VF225_03060"
     CDS             complement(3243..4688)
                     /locus_tag="VF225_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7144262.1"
                     /translation="MPSHLVRRIVLTGAVVLTATACSGGTAASPGQSASSAGSASDWP
                     LFGYTAARANSGPSATGITSADVGRLWRQRVLLDGTVDSSPIYLHAVRVRGAVHDVFF
                     VTTTYGRTEAIDASSGRVLWRYTPAGYSSWVGTARITTATPAADPGRKWIYAASPDGR
                     IQKLSVADGHLAWRTRITLLPSREKIASALNYWHGRVIATTGGYIGDAPPYQGHVALL
                     AASNGRLSSVWNSLCSNRHQLISPSSCASSDSAIWGRAGAVVDTATGGLLVATGNGDW
                     NGRTNWGDSTVHLSADASRVLGSWTPTNEAALQSSDLDLGSTSPVFLGSGYLAQGGKD
                     GKIRLLRLSKLSPAGRTGGELQTISTPGTTDLFTAPAVWHADGQTWFFVADNAGLAAW
                     TLGGGRLHPAWSTRTPGTSPVVAGGLLYVYDPNGGLGIYLPRSGKKLRTLAAAGGHWN
                     SAIVADGRIALPEGDANQHALSGVLDIYRLG"
     gene            complement(4971..6176)
                     /locus_tag="VF225_03065"
     CDS             complement(4971..6176)
                     /locus_tag="VF225_03065"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX7144263.1"
                     /translation="MPFYQRLGDVPRKRHIQFRDNGTLLTEEVAGMEGFVGMESILYH
                     LQSPCRVMKIGDFEEIKRDEWVPDAHAHRHFKTYDVDAKGDEITGRRLLMWNNDVEIS
                     LARPKEQMDYFFRNGEGDEVVFVHEGTGTLETNFGNVPYKDGDYIIIPRGTTYRFKPE
                     GEQRYLVFETPGLIEIPKRYRNQYGQLLEGAPYYHRDIHPPTELNTHRDKGEFPVKVR
                     VRGGYQTYVLDYHPFDVVGWDGYLYPWTFSVHDFEPITGRIHQPPPSHQTFQGRNFVI
                     CSFVPRRLDYDPQAVPIPYHHSNLQSEEMIYYVSGKFGSRKGVEVGSVTLHPSGIPHG
                     PHPGLAEKSIGMTETHELAVMCDTFYPLKLTKFAKDLDDGKYAYSWYEEPGAPATADE
                     DPAGVTSHF"
     gene            complement(6179..7174)
                     /locus_tag="VF225_03070"
     CDS             complement(6179..7174)
                     /locus_tag="VF225_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7144264.1"
                     /translation="MQAEVVVVGAGIMGSATAYALAREGRDVLLLEQFRVGHGHGSSH
                     GRSRIVRLAYPEVEFVELAKEAFVGWRELEQESGQELLELNGLLELVVSSDQSTADAL
                     AAAGAEYELLDAAGARSRWQVGVPDGWTVLFQPEAGIVRADLAHRAFVDGALAHGARL
                     EEEARVESLDELDAKVIVVTAGPWIGNFFPDLPVRTTRETLAYFRREGAPLPVVAQLD
                     PDTRGHLIYSLHDPLHGLKAGVHHGGAAAGPDEPGGPDPAVVERVAEWVARTYADADP
                     DPVGVETCMYTTTPDERFILERRDNVVIGSACSGHGFKFAPAIGSRLAGMAADIL"
     gene            complement(7189..7941)
                     /locus_tag="VF225_03075"
     CDS             complement(7189..7941)
                     /locus_tag="VF225_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7144265.1"
                     /translation="MSVDMENLMPIGEFASASQLSQKALRLYAENALLPPAWVDPDSG
                     YRYYRPDQLREATLIALLRRTGMSLADIRSFMSDPSVERLDEYERQVADEFAECRRVL
                     RYVKRVLKEEPMYDVFTKSVPETTYVSRSRRVLVPELTSFICSAFDELGQSGDGPSFV
                     LYHGPVNAEEDGPVEVCVPKTEGQNRLPAGEVAFTEISGSQCDFPEILGAYEAVYRWA
                     KEKGREPDGPPREIYLSAADEELRMEIALPLR"
     gene            complement(7967..8779)
                     /locus_tag="VF225_03080"
     CDS             complement(7967..8779)
                     /locus_tag="VF225_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7144266.1"
                     /translation="MRLCMFTPRDLQLERGWPGIIEGDTVVQLAAQTLQAFFTGGGTA
                     RRHAEYPLAEVDFRPPVLHPPSVRDFYAFEQHVMTARALRGQEVPPEWYEIPVFYFSN
                     PAAIYGAEDEIPYPPDTKELDYELEAAAIIGADEQIAGFTIMNDWSARDLQRAEMKVG
                     LGPAKGKDFATSLGPILVTPDEFDGKSGRMVARVNGEERSRGNLADMYHSWDAIVAHA
                     ARNTHLRPGDVLGSGTVGTGCILEHGDGRWLQRGDVVELEVEGIGILRNGVA"
     gene            complement(8931..>9684)
                     /locus_tag="VF225_03085"
     CDS             complement(8931..>9684)
                     /locus_tag="VF225_03085"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HEX7144267.1"
                     /translation="VLMDRAPADAEQAARAALARVEPPVTLKRLRMRQAGPRQFMDVV
                     IAVPPGAAVGQGHAAADRVEDALAAALPGSDVVVHVEPQEDEAAIRDRAHAAALGVPS
                     VREVHNVSVLDVGGRTEVSLHLKLPGEVTLEEAHDVANQVESAIAAAVPEVDSVQTHI
                     EPLGEAVEGKPMVDDHAERDVVLRIVREVTGEEPRELRFVRTDEGLLAYLTLGLDPTS
                     PLAEAHARASEIEERIRRARPDIADVIVHTEP"
BASE COUNT         1560 a         3256 c         3231 g         1637 t
ORIGIN      
        1 gccgagccgc cgccgacaag gccgctgaac caggcgccca gacagcggtc tacgcaatcg
       61 agcaactgcg ttcctgggac tggttgatgt ggacgccggg cctcacggtg atgatggtcg
      121 ccgcaggcct gggcggactg cggacacgcg cactaccgaa gccgctgtcg tggacctcag
      181 tcgtcctcgg cgtcgcattc ttcacgccgc tcggcttctt tgccttcttc atcctcccgc
      241 cctggatgat cgccaccgga gtcgtgctct atcgacggca gcgggtcgcg gcggcgggcg
      301 ttcccgctta gagctacgcg ggaaccggca gaccctcgat cgcgcgcagg gcgcgctcga
      361 tctcgtcctc gtccagggcg acatcctcga gcttgcccgc gaagtagttg tcgtaggcag
      421 ccatgtcgaa gtggccgtgg ccggaaaggt tgaacaagat ggtgcgctcc tcgcctgcct
      481 cgtcggccgc cttcgcaacc tgaagcgtcc cgtgaatcgc gtgcgcggct tcaggcgccg
      541 ggaggatccc ctcgtttccc gcgaagagca cggccgaggc gaagacatcg ttctggagat
      601 atgcctcggc ccggatcagt ccgtcccgca ggagctgcga gatcagcgga gcaactccgt
      661 ggtaacggag gccgccggcg tggatcgggg ccgggacgaa gtcgtgcccg agcgtgtgca
      721 tcggtaccag cggcgtcagc ttgctcgtgt cgccgaagtc gtacgcgaac agcccgcgcg
      781 tcagcgtcgg gcacgctgcc ggctcgcatg cgagcacgtc gatctcgcgg cccgcgatct
      841 tctcgcggag gaacggaaac gcaaggccgg cgaagttcga gccgccgcca acgcaaccga
      901 tgacgacgtc ggggaaggcg cctgcgagct ccatctgcgc gagcgcctcc tggccgatca
      961 cggtctgatg gaggagaaca tggttgagga cgctccccag cgagtaggcg gtgtcgtcgc
     1021 gtccagccgc gtcctcgacc gcctccgaga tcgcaattcc gagactcccc ggcgagtcag
     1081 ggtgctcctc gagaatcgcg cgtccggact gcgtctcatc ggaagggctc ggcacgatct
     1141 cggcgcccca cgccttgatc atcgaccgcc gggcaggctt ctgttcgtac gagacccgaa
     1201 ccatgtacac cttgcattcg agcccgatca gcttgcatgc aaacgaaagc gcactccccc
     1261 actggcctgc gccggtctcc gtggcgagac gcgtccgccc ttccttcttg ttgtagaacg
     1321 cctgcgcgac cgcggtgttc ggcttatggc tccccggcgg gctgacgcct tcgtacttgt
     1381 agaagatgcg cgagcgggtc ccgattgccg cctccaggcg cgacgcacgg tagagcggcg
     1441 tcggacgcca gaggctgtag atgtccagga cctcctccgg gatggcgatc tccgcctcct
     1501 cgctcacctc ctggccgatc agctccatcg ggaacagggg cgcgagatcg tccggcccga
     1561 cgggctgccc ggtgcccgga tgcagcaccg gctgcagagc cgtgggcatg tcagaacgga
     1621 tgttgtacca gtgccgcgga agctgcgatt cctcgaggag gaacttggtc tgactaccgg
     1681 cgcgactccg catctgctgc acgccgcgag catagaacct cagcccgacc ggtgcagcct
     1741 ctggcaggct gcgcagggcg aaacgcgtac accgtcccct cggtgtcggc tccgagcacg
     1801 gttccggcaa ccgtgatcat cgcggcgacg gtgccgccgg tcgtcgcgta ggaccagagg
     1861 ctgacgccgt tggcggcgtc gagcgcatag aaaccgcccg gcgttccgcc ggtggcaaac
     1921 aggacgtccc cgacgatgag cggcttcgcc tggttcccgt cgccgagggc cctcgaccag
     1981 atcggccgga agccgcagcc ggcgtcgacg tggaaggccc gtacgccgtt gccaagcctc
     2041 ttcccgtcat cgccgtagag gacggactgc gcggagtaga tcatctgctt ctcgaccgac
     2101 caggcgggct cgccgatgaa cggcgccaca ccgtcgccga gcgggatgaa gacgattgaa
     2161 ccccgtgaca ggtgctcgcg gtcgaggatg tagagcgtgc cgttcttgtt gtttgccgcg
     2221 gccagcggcg ggcaggtttc cggttggaag agcaatgcgg cggcgccgaa gtcgtcgtcg
     2281 ccggtcggag gaatgtcggg aagttgcgag gagtcgagca ccctcgtgag gtcagggctc
     2341 aatgcgacga tcctgtctcc gtagcccgcg ttgtcgacga agcaggaaca ctcgtccgac
     2401 cagacatgtg agttgccaac tcccgtgtag acgaccccgg tcgtgggagc gatcgagact
     2461 ccgccccaac cccacatccc gccgaggttg tccggaccgg gcaccggatc ccacgtcgtg
     2521 cttgctctcg cggcatcgag cgagatcgcc aacaggcttc cctccggaaa tccgtctgtg
     2581 ctgccgacgt cgcagtagga agcgattggc acgtacagcc ggtcacgggc gatgcgcaga
     2641 cctccccaga cgtattcgat gtcggggcgt gacacgactt gccgcggata gcccgtcgct
     2701 tcttcgcccg tcgcgagcga gagcgcgtgg agctgtccgg tggcgccgat gacatacatg
     2761 agcccacgag cgaggtcgat tgcgccggtg gaggtgatac cccaggtgcc acagagctct
     2821 gtggtcgtgg tcccgagctc gcgctgccac agcaccgcgc cggtcgtgac ggccaaggcg
     2881 tacacggacc ccgcttcagt cgcaacgtac gccacctgtt ctccgtcgac tcgagcggag
     2941 agaggcgagg cgtagacagg gccgtccagc ccggccttcc acgccggagc caggcctggg
     3001 acggttcgaa gcgtcaggag ggtcgagggg acggcattcc ggagttggtt gtcgtagccg
     3061 taactcgtcc acgcgaccgg atcggacggc gtttccgcct tggcctgcgg ccttggcttc
     3121 gcgcccgcac ctgaaacgac gaccagggag aggagcgaga ggcctaggac gcgccgcagc
     3181 atcggccgcg gagagtagct gcgagattat ggccgtgccg gttcgtcggt ttcgaaagcg
     3241 cgctaaccga gacggtagat gtcgaggacc ccgctcagag cgtgctggtt tgcgtctcct
     3301 tcgggaagcg cgatgcggcc gtcggcgacg atggcgctgt tccagtgccc gccggcagca
     3361 gcgagcgtcc tgagtttctt gccgctgcgc ggcaggtaga tgccgaggcc gccgttcggg
     3421 tcgtagacgt agaggaggcc gccggcgaca accgggctcg ttccgggagt acgcgtcgac
     3481 cacgcggggt ggagccggcc gccgcccagt gtccaggccg cgagaccggc gttgtctgca
     3541 acgaagaacc acgtctggcc gtcggcgtgc cagacggccg gggcggtgaa cagatcggtc
     3601 gtcccgggtg tcgagatcgt ctgtagctcg ccgccggtac gcccggccgg cgagagcttg
     3661 ctcagacgga ggagccggat cttgccgtcc ttgccccctt gcgcgagata gccgctgcca
     3721 aggaacacgg gagaggtcga acctaggtcg aggtcgctcg actggagcgc cgcctcgttc
     3781 gtcggagtcc agctgccaag gacgcgcgaa gcatccgcgg acagatgcac ggtggaatct
     3841 ccccagttgg ttctgccgtt ccagtctccg ttgccggtcg caacgagaag tcctccggtg
     3901 gcggtgtcga cgacggcgcc cgcccgaccc cagatcgcgg agtcgctgga ggcgcagctg
     3961 ctcggcgaga tcagctgatg ccggttcgag cagagcgaat tccagacgct cgaaagcctt
     4021 ccgttgctcg cggcgagcag cgccacatga ccctggtagg gcggcgcgtc gccaatgtag
     4081 cctccggtgg ttgcgatcac gcgcccgtgc cagtagttga gcgcagaggc gatcttttcc
     4141 cgcgacggga gcagagtgat tcgagtccgc caggcgaggt gaccgtcagc caccgaaagc
     4201 ttctggatcc gcccgtcggg cgacgccgcg tagatccact tgcggccggg atcagcggcg
     4261 ggtgtcgcgg tcgtgatccg tgccgtcccc acccaggagg aatagcccgc cggcgtgtag
     4321 cgccagagca cgcggccgct ggacgcgtcg atcgcttccg tacggccata cgtcgtcgtg
     4381 acgaagaaga catcgtggac ggcgccgcga actcgaacgg cgtgcaggta gatcggcgac
     4441 gaatccaccg tcccgtcaag cagcacacgc tgccgccata agcggcctac gtcggccgac
     4501 gttattccgg tcgctgaagg cccgctgttc gcacgtgctg ccgtgtaccc aaacaatggc
     4561 cagtcgcttg ccgagccggc ggagctcgcg ctctggcccg gcgaggccgc tgttcctcca
     4621 ctgcaggctg tggccgtgag cactacagcc ccggtcagca cgatgcgcct caccaggtgc
     4681 gatggcatca cggtttaggc cacacgcctg agcctgaggg cgcgctccca gcgtgcgtgt
     4741 tcgacgcgaa gcggcgcgtt cagatccctg caatcgtggc atcgcagcga agccgtccgt
     4801 cgcaggggct cgggcaactc gcctccacag gccaggcaga tgaacgctct gatcaacatg
     4861 agccgaacga tgaccgacac atgttttgcc ggcatcgagg gcagccgcaa tctgaatgcc
     4921 gggtagccga actctcggtc ttcccgctag cccttagagc gacttggccc ctagaagtgc
     4981 gaggtgactc cggccggatc ctcgtcggcc gttgccggcg cgccaggctc ctcgtaccac
     5041 gagtacgcgt acttgccgtc atccaggtcc ttggcgaact tcgtcagctt cagcggatag
     5101 aaggtgtcgc acatcacggc aagctcgtgc gtctccgtca tcccgatcga cttctcggcc
     5161 aggcccgggt gtgggccgtg gggaatgccc gaggggtgga gcgtcacgga gccgacctcg
     5221 acccctttgc gcgagccgaa cttgccggag acgtaataga tcatctcctc cgactgcagg
     5281 ttcgagtggt ggtacgggat cggcaccgcc tgcgggtcgt agtcgagtct gcgcgggacg
     5341 aaggagcaga tgacgaagtt ccgtccctgg aaagtctggt gtgagggcgg cggctggtgg
     5401 atgcggccgg tgatcggctc gaagtcgtgc accgaaaacg tccacgggta caggtagccg
     5461 tcccacccca cgacgtcgaa ggggtgatag tcgagaacgt acgtctggta gccgccgcgc
     5521 acgcgcacct tgactgggaa ctcccctttg tcgcgatgcg tgttcaactc cgtcggcgga
     5581 tggatgtcgc ggtggtagta cggcgcaccc tcgagtaact gcccgtactg gttgcggtag
     5641 cgcttcggga tctcgatcag acccggagtc tcgaagacga ggtagcgctg ctcaccctcc
     5701 ggcttgaagc gatatgtagt gccgcgcggg atgatgatgt agtcgccgtc cttgtacggg
     5761 acgtttccga agttcgtctc gagcgtgccg gtgccctcgt ggacgaagac gacctcgtcg
     5821 ccttcgccgt tgcggaagaa ataatccatc tgctccttcg ggcgcgcgag cgagatctcg
     5881 acgtcgttgt tccacatcag caggcggcgt cccgtgatct cgtcgccttt ggcgtcgacg
     5941 tcgtaggtct tgaagtgacg gtgcgcgtgg gcgtccggaa cccattcgtc gcgcttgatc
     6001 tcctcgaagt ccccgatctt catcacgcgg cagggcgatt ggaggtggta gaggatcgac
     6061 tccattccga cgaagccctc catgcccgcg acctcttcgg tgaggagagt tccgttgtcg
     6121 cgaaactgga tgtgacgctt gcgagggacg tcgccgagac gctggtagaa cggcacggct
     6181 aaaggatatc ggcggccata cccgcgaggc ggctacctat cgcgggcgcg aacttgaagc
     6241 cgtgtccact gcacgctgac ccgatcacga cgttgtcgcg ccgctcgagg atgaagcgct
     6301 cgtccggagt ggtcgtgtac atgcacgtct ccaccccaac cggatccggg tccgcatcgg
     6361 cgtacgtccg ggcgacccac tcggcgactc gctccaccac tgctggatca ggcccgccgg
     6421 gctcgtccgg tccggcagcg gctccgccgt gatgcacgcc ggccttcagg ccgtggagcg
     6481 ggtcgtgcag cgagtagatc aagtgaccgc gcgtgtccgg atccagctgc gcaactaccg
     6541 gaagcggggc gccttcccgg cggaagtacg caagcgtctc gcgcgtggtg cggaccggca
     6601 ggtcggggaa gaagttcccg atccacgggc ctgcggtcac cacgatcacc tttgcgtcga
     6661 gctcgtcgag agactcgact cgcgcctcct cctcgagtcg ggcgccgtgg gccagcgcgc
     6721 cgtcgacgaa ggctcggtgc gcgaggtcgg cgcggacgat gccggcctcc ggctggaaga
     6781 gcacggtcca cccgtcggga acgccaacct gccagcggct ccgtgctcca gccgcgtcga
     6841 gcagctcgta ctcggctccg gcggccgcca gcgcgtctgc cgtgctctgg tccgagctca
     6901 cgacaagctc gaggaggccg ttcagctcga gcagctcctg cccagactct tgctcgagct
     6961 ctcgccagcc cacgaatgcc tccttcgcca gctcgacgaa ctcgacctcc ggatacgcaa
     7021 gccgcacgat gcgcgaccgg ccgtggctcg agccgtgccc gtggccgacg cggaactgct
     7081 cgaggagcag gacgtcccgc ccttcgcgcg ccaacgcgta cgcggtcgcc gaacccatga
     7141 tccccgcgcc gacgacaacg acctctgcct gcacgggcct atcttcgcct agcggagcgg
     7201 gagtgcgatc tccatacgca actcttcgtc ggctgcactg aggtagatct cacgtggcgg
     7261 tccgtcgggt tcgcggccct tctccttggc ccaccggtag acggcctcgt acgcgccgag
     7321 gatctcgggg aaatcacact ggctgccgct gatctccgtg aaagcgacct caccggccgg
     7381 caggcggttc tgaccctcgg tcttcgggac gcagacctcg acggggccgt cctcttctgc
     7441 gttcaccggg ccgtgatata ggacgaacga gggaccgtcg ccggattggc ccagctcgtc
     7501 gaaggccgaa cagatgaacg aggtgagctc gggaacgagt acgcgcctcg agcggctgac
     7561 gtacgtcgtc tcgggaacgc tcttcgtgaa tacgtcatac attggctcct ccttcaggac
     7621 tcgcttcacg tagcggagaa cccgccggca ttcggcgaac tcgtcggcca cctgccgctc
     7681 gtactcgtcg agtcgctcga cgctcgggtc gctcatgaac gagcgaatgt cggccagcga
     7741 cattccggtg cgccgcagca gcgcgatgag cgttgcctcg cgcagctggt ccgggcggta
     7801 gtagcggtag cccgagtccg gatcgaccca cgcgggcggc agcagggcgt tctccgcata
     7861 gaggcgcagg gccttctgcg acagctgcga ggccgaggca aactcgccga tcggcatcag
     7921 gttctccatg tcgacgctca cgatggcgtc tccccttggg gcagagtcaa gcgacaccgt
     7981 tccgcaggat gccgatcccc tcgacctcca gttctacgac gtcgccgcgc tgcagccagc
     8041 gaccgtctcc gtgctcgagg atgcagccgg ttccgacggt gccggagccg aggacgtcgc
     8101 cgggccgcag gtgcgtgtta cgtgcggcgt gcgcgacgat cgcatcccag gagtggtaca
     8161 tgtcggcgag gttcccgcgc gaccgctcct cgccgttgac gcgtgcgacc attctgccgc
     8221 tcttcccgtc gaactcgtcg ggcgtcacga ggatcggccc gaggctcgtg gcgaagtcct
     8281 tccccttggc gggaccgagc cccaccttca tctccgcccg ttggagatcg cgcgcggacc
     8341 agtcgttcat gatcgtgaag ccggcgatct gttcgtccgc gccgatgatc gccgcagcct
     8401 cgagctcgta gtccagctcc ttcgtgtcgg gtgggtacgg gatctcgtcc tccgccccgt
     8461 agatcgccgc ggggttggag aagtagaaga ccgggatctc gtaccactcg ggcggcacct
     8521 cctgaccgcg cagcgcgcgg gcggtcatca cgtgttgctc gaaggcgtag aagtcacgga
     8581 cggagggtgg gtgcagcacg ggcgggcgaa agtcgacctc ggcgagcgga tactccgcgt
     8641 gcctgcgagc cgtgccgcca ccggtgaaga acgcttgcag cgtttgcgcc gcgagctgga
     8701 cgacggtgtc gccctcgatg atccccggcc agccgcgctc gagctgcaag tcgcgcggcg
     8761 tgaacatgca gagtctcatg acactctcgt cgcgaagtcg tacccgtgcc tggcaccggg
     8821 acgcgtccgg cgagcccgca cgccatcagg caacgctgtc tcgcatgcgc gactcgtaac
     8881 agtttcgtac ccgtgcctgg caccggtacg ttccggaagc ccgtcgcgca tcacggctcc
     8941 gtgtgcacga tcacgtcggc gatgtcggga cgcgcgcggc ggattcgctc ctcgatctcg
     9001 ctggcacggg catgcgcttc ggccagcggg ctcgtcggat cgagccccaa ggtcaggtag
     9061 gcaagcaagc cctcgtcggt gcggacgaac cgcagctcac gcggctcctc ccccgttacc
     9121 tcgcggacga tgcggagcac gacatcgcgt tcggcgtggt cgtccaccat tggttttccc
     9181 tccacggcct cgccgagcgg ctcgatgtgc gtctgcaccg agtcgacctc cgggacggcg
     9241 gccgcgatcg cgctctcgac ctgattcgcc acgtcgtgcg cctcctcgag cgtcacctcc
     9301 ccgggaagct tcagatgcag cgacacttcc gtccgcccac cgacatcgag gacgctgacg
     9361 ttgtgcacct cgcgcacgga cggaacgccg agcgccgccg catgcgctcg gtcgcggatg
     9421 gctgcctcat cctcctgcgg ctccacatga acgacgacgt cgcttccggg caacgccgcg
     9481 gcgagcgcat cctccacgcg gtccgccgct gcatgaccct gccccacggc agcgcccggc
     9541 gggactgcga tgaccacgtc catgaactgc cgcggccctg cctgcctcat gcgaaggcgc
     9601 ttgagcgtca ccggcggctc gaccctggcg agcgcagcgc gggccgcctg ctctgcatcg
     9661 gccggcgcac gatccatcag gacg
//
LOCUS       DASVEU010000080         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_448730,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000080 DASVEU010000000
VERSION     DASVEU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448730"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..510
                     /gene="rplQ"
                     /locus_tag="VF225_03090"
     CDS             115..510
                     /gene="rplQ"
                     /locus_tag="VF225_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7144268.1"
                     /translation="MRHRRSGKKLGRDSAHRKALYSNLAGALIEHGRIKTTVAKAKAV
                     KPFAEQMITLGKRGDLHARRLALAELRSQDVVHLLFADVAPRFADRPGGYTRIVKLGP
                     RLGDAAEMVYLELVDYVPAGASPSASATS"
     gene            523..825
                     /locus_tag="VF225_03095"
     CDS             523..825
                     /locus_tag="VF225_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7144269.1"
                     /translation="MLLLGAILLAIFVLPSPWGIVAVIAGGLIDIGESLVLLKWTRRR
                     KSTVGVETLVGQKGVVSTLTQVRVAGELWEARSDRVLVPGEEVVVRGVEGLTLLVD"
     gene            complement(861..1337)
                     /locus_tag="VF225_03100"
     CDS             complement(861..1337)
                     /locus_tag="VF225_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144270.1"
                     /translation="MTDKQAFLLQRVQPAMVGLIDGSLSTLAPIFSVAVVTHKPHFAF
                     VAGVATAFGAGISMAFSEGLSDTGELTGRGNPMVRGAITGAGTFVGGILHTLPFLIPH
                     YQAAIYSAIAVVALELLILAWLRWKFFEVGFVRALASVTLGGVIIAAVSAALGSVG"
     gene            1416..2129
                     /gene="truA"
                     /locus_tag="VF225_03105"
     CDS             1416..2129
                     /gene="truA"
                     /locus_tag="VF225_03105"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7144271.1"
                     /translation="MRLKLTLEYDGTDFHGWALQPGLRTVEEVVRDALGVFPSFADLA
                     VAGRTDAGVHALGNVVSVDVDGGPPAARVAGALNAALPDDVAIVSASEMAPDFHARRA
                     ARSRSYRYRIWRRREPSPFEQRRSWWYQRPLDEERLAVAADLLVGVHDFRAFTPTETQ
                     HQAFRRSVLAAAWYRRGDALEFEITADAFLRHMVRTLVGTMLELPPERIAPLLEGRPR
                     NEAGPTAPPCGLYLERVIY"
     gene            2134..2436
                     /locus_tag="VF225_03110"
     CDS             2134..2436
                     /locus_tag="VF225_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7144272.1"
                     /translation="MADYFLVKHVPGPRWDHSRRRREQAGWDEHAAFMDGLTEEGLIF
                     LGGPVGTGDGDYALLVFDAKSDAEVRARLAGDPWANGTLVVESIEPWSVWLRSPPG"
     gene            2447..3082
                     /locus_tag="VF225_03115"
     CDS             2447..3082
                     /locus_tag="VF225_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7144273.1"
                     /translation="MRFPVVLFDLDGTVIDSGAIILASMRHAAKEVLGAEIPDEELMA
                     AVGGPGLEAQMHALSPDKVDQLVDVYRAHNEPLHDELVCCAGMDEVLVRLKDEGRRLG
                     IVTAKRRQTVELAFARIPIAHLFETVVGGDETARHKPDPEPLLLALERMGAGAGEAAY
                     VGDAPFDVKAAKAAGLFSVGVTWGGIHARERLEGERPDALVDTAEELYAVL"
     gene            3069..5084
                     /gene="ligA"
                     /locus_tag="VF225_03120"
     CDS             3069..5084
                     /gene="ligA"
                     /locus_tag="VF225_03120"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7144274.1"
                     /translation="MPSSKEEKRAAELRDVLDYHLYRYHVLDDPEVTDAEYDRLYDEL
                     AALEGANPELATPGSPTQRVGAPPSDKFQKVAHPSPMGSLEKVTTDEALEKWHQDVCK
                     RLGTSDVAYVTEPKIDGLSINLVYEDGLFVRGATRGDGRLGEDVTPNVRTIKAISMRM
                     QLAAGETPPPLLEVRGEVYLPISGFNQLNERLIAEGKKPTPNPRNAAAGSLRLKDSTI
                     TAERPLSIWVHGLGLREGLPVDGHWESLEWLRAHGFRTNPYAERHETVESVAAACRDW
                     EKRRIELDYEIDGIVIKVDSFDQQRRLGALHDRPRWARAFKWAPMTAETKLNKIHIRV
                     GRTGALNPWAQLEPVEVGGVTVSQATLHNEEDINRKDIREGDNVIVQRAGDVIPQVVG
                     PVLPHSKGTKPFKMPKQCPLCGVDVVKPEGEAMHRCPNRACPSRGLESLINWVQAAAD
                     IEGVGEQSVRRLWDLGLVRSLPELYRLTKEQLMELEGYGEISATSAIESIAASKQVPF
                     NRVLFGLNIPDVGWVTAQNLARHFENVDRLLSAAQEDILEVEGIGPERAEAIAEWFSD
                     EANRALVEELRSLGLRFEIGDELKPVEGPLTGNTFVITGTLEGFTRDEAAAALEAKGA
                     KVVNSVSGKTTGLVVGEEPGNSKLTKARKVEVPILSEADLVELLKRT"
     gene            complement(5129..>5694)
                     /gene="dacB"
                     /locus_tag="VF225_03125"
     CDS             complement(5129..>5694)
                     /gene="dacB"
                     /locus_tag="VF225_03125"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX7144275.1"
                     /translation="RTGTADAWSEPLADVSSPTLWTMLRFMDRESDNFTAEMLLKQLG
                     LTELGRGTSAAGAAVVTQALTDAGVPMTGVRIVDGSGLSRLDRLTANALGSLLKIAWA
                     DPTVGAALLASLPVAGVNGTLQHRLQKPPAKGNVLAKTGTTDTASSLSGYVSGRYAFA
                     VVQNGHPLSYWWARRAQDRFAQVLAGS"
BASE COUNT          967 a         1842 c         1991 g          894 t
ORIGIN      
        1 gccgcgatcc cgaacttcgg caagaagtcg gtcgaagagg tcaaggagac tctgtcggcg
       61 cacggactga ctctgcgcgg cgacgagagc ggcggcggag gcgcgctcta accaatgcgc
      121 catcgccggt ccggcaagaa gctcgggcgc gactcggcgc accgcaaggc gctctactcg
      181 aacctcgcgg gcgcgctgat cgagcacggc cgcatcaaga ccaccgtggc caaggccaag
      241 gcggtcaagc cgttcgcaga gcagatgatc acgctcggca agcgcggcga cctccacgcc
      301 cggaggctcg cgctcgccga gctgcgctcc caggacgtcg tccacctgct gttcgcagac
      361 gttgccccgc gcttcgcgga ccggcccggc ggctatacgc ggatcgtcaa gctcgggcct
      421 cggctaggtg atgccgcgga gatggtctac ctcgagctcg tggattacgt gccggccggc
      481 gcatcgccgt cggcctcagc aacgtcttag cgcaaggagc tcatgctcct actcggagcg
      541 atcctgctgg cgatcttcgt cctgccctcg ccctggggga tcgtggccgt cattgccggc
      601 ggactgatcg acatcggcga gtcgctcgtc ttgctgaagt ggacgcggcg ccgcaagtcc
      661 accgtcggcg tcgagacgct cgtcggacag aagggtgtcg tcagcacgct gacgcaggtc
      721 cgggttgcgg gtgaactctg ggaagcccgc tcggatcggg tgctcgttcc cggcgaagag
      781 gtcgttgttc gtggtgtgga gggattgacg ctgctcgtgg actgagtccc gatgctcgcg
      841 aacgagtcac tgagcctgga ctagcccacg gagccgagtg ccgcgctgac ggctgcgatg
      901 atcacgccgc cgagcgtgac ggacgcgagc gcccggacga aaccgacttc gaagaacttc
      961 caccgcagcc acgcgaggat gaggagttcg agcgcgacga ctgcgatcgc cgagtagatc
     1021 gccgcctggt agtgcgggat caggaacggc agggtgtgca ggattccgcc gacgaaggtt
     1081 ccggcgccgg tgatggcgcc gcggaccatc gggtttcctc ggccggtcag ctcgcccgtg
     1141 tccgagagcc cttcggagaa cgccatcgag atcccggcgc cgaacgcggt ggcgacgcct
     1201 gcgacgaagg cgaagtgcgg cttgtgggtg acgaccgcga cggagaagat cggcgccagg
     1261 gtcgagagcg agccgtcgat gagtcccacc atcgccggct gcactcgctg gagaagaaag
     1321 gcttgcttgt ccgtcattga ttagagtgta tcaaatcgat cgcaagccag tagaacataa
     1381 ggcaaacctg acaaacagag tcaggggggc ctgacgtgcg gctgaaacta acgctcgagt
     1441 acgacggcac cgatttccac ggctgggcgc tgcagccggg gttgcgcacg gtcgaggaag
     1501 tcgtgcgtga cgcgctcggt gtctttccct cgttcgccga cctcgccgtt gcggggcgaa
     1561 ccgacgcagg cgtgcacgca ctcggcaacg tcgtcagcgt cgacgtcgac ggcggcccgc
     1621 ccgcggcacg cgtcgcggga gcgctcaacg ccgccttgcc ggacgacgtt gcgatcgtct
     1681 ccgcaagtga aatggcgccc gacttccatg ctcggcgtgc ggcgcgttcg cgtagctacc
     1741 gctatcgcat ctggcgccgc cgtgagccgt ctccgttcga gcaacggcgt tcctggtggt
     1801 accaacgccc gctcgacgag gaacgcctgg cggtcgcggc cgaccttctc gtgggcgtgc
     1861 acgacttccg cgcgttcacc ccgaccgaga cgcagcatca ggcgttccgg cggagcgtgc
     1921 tggcggccgc ctggtaccgg cgcggcgacg cgctcgagtt cgagatcacg gcggacgcct
     1981 tcctcaggca tatggtgcgg accctcgtgg gcacgatgct cgagctacct cccgagcgga
     2041 tcgcgccgct gctcgaagga cgcccgcgca atgaggcggg gccgacggct ccgccctgcg
     2101 ggctctatct cgagcgcgtg atctactgag gcggtggccg actacttcct cgtcaagcac
     2161 gttcccgggc ctcggtggga tcactcacgt cgccgacgag agcaggcagg ctgggacgag
     2221 cacgcagcgt tcatggatgg gctgaccgag gaaggtctca tcttcctggg tgggcccgtc
     2281 gggaccggtg atggggatta cgcgttgctc gtcttcgacg caaagagcga cgccgaggtt
     2341 cgcgctcgcc tcgccggcga tccgtgggcg aatggcacgc tcgtcgtcga gagcatcgag
     2401 ccctggtcgg tctggttgcg ttctccccct ggctagcatc aaccgcatgc ggtttcctgt
     2461 cgtcctcttc gatctcgacg gcacagtcat cgactccggc gcgatcatcc tcgcgtcgat
     2521 gcggcacgcc gccaaggagg ttctcggagc tgagattccc gacgaggagc tgatggcggc
     2581 ggtcggcggc cccggtctcg aggcgcagat gcatgctctc tcgccagaca aggtcgacca
     2641 gctcgtcgac gtctatcgcg cgcacaacga gccgctgcac gacgagctcg tctgctgcgc
     2701 ggggatggac gaggtgctgg ttcgtctgaa ggacgagggg cggcggttgg ggatcgtcac
     2761 cgcaaagcgg cgccagaccg tcgagcttgc gttcgcgcgg attccgatcg ctcatctgtt
     2821 cgagacggtc gtcggcggag acgagacggc gcggcacaag cccgatccgg agcccctatt
     2881 gctcgcccta gaacggatgg gcgcaggcgc gggtgaggcc gcttacgtcg gtgacgcgcc
     2941 gttcgacgtc aaggcggcta aggctgcggg tctgttctcg gtcggcgtga cctggggcgg
     3001 catccatgct cgtgagcgac tcgagggcga acgcccggac gcgctcgtcg acaccgcgga
     3061 ggagctgtat gccgtcctct aaggaggaga agcgcgcagc ggagcttcgc gacgtcctcg
     3121 attaccacct ctaccgctac cacgtgctcg acgaccccga ggtcacggac gccgagtacg
     3181 accgcctcta cgacgagctc gccgcgctcg agggagcgaa cccggaactg gcgactccgg
     3241 gctcgccgac gcagcgcgtg ggcgcacctc cgtcggacaa gttccagaag gtcgcgcacc
     3301 cctcgccgat gggctcgctc gagaaggtga caacggacga ggcgctcgag aagtggcacc
     3361 aggacgtgtg caagcgactc ggcacgagcg acgtcgcgta cgtgacggag ccgaagatcg
     3421 acgggctctc gatcaacctc gtctacgagg acggactctt cgtacgcggc gcgacgcgcg
     3481 gggacggccg tctgggggag gacgtcacgc cgaacgtgcg cacgatcaag gcgatctcga
     3541 tgcgcatgca actcgccgcg ggagagacgc cgccaccgct gctcgaagtg cgtggcgagg
     3601 tctacctccc gatcagcggc ttcaaccagc tcaacgagcg gttgatcgca gaagggaaga
     3661 agccgacgcc gaatcctcgc aacgcggcgg ccggatcgct ccggctcaag gactcgacca
     3721 tcaccgccga gcgtcctctg tcgatctggg tccacggcct cggtctgcgc gagggtcttc
     3781 ccgtggacgg tcactgggag tcgctcgagt ggctgcgggc gcacggcttc cgcacgaacc
     3841 cgtacgcgga gcggcacgag accgtcgagt ccgtcgcggc agcatgtcgt gactgggaga
     3901 agcggcgcat cgagctcgac tacgagatcg acggcatcgt gatcaaggtc gattcgttcg
     3961 accagcagcg gcgactcggc gcgctgcacg acaggccgcg ctgggcgcgc gcgttcaaat
     4021 gggcgccgat gacggccgag acgaagctga acaagatcca catccgggtc ggccgcacgg
     4081 gtgcgctgaa cccgtgggca cagctcgagc cggtcgaggt cggcggcgtc accgtgtcgc
     4141 aggcgacgct gcacaacgaa gaggacatca accgcaagga cattcgcgag ggcgacaacg
     4201 tgatcgtcca gcgtgcgggc gacgtgatcc ctcaggtcgt ggggcctgtg ctgccccatt
     4261 cgaaagggac gaagccgttc aagatgccga agcagtgccc actctgcggc gtggacgtcg
     4321 tcaagccgga gggcgaagcc atgcatcgct gcccgaaccg tgcttgtccc tcgcgtgggc
     4381 tcgagtcgct catcaactgg gtgcaggcgg ccgccgacat cgagggtgtc ggcgagcaat
     4441 ccgtgcgccg gttgtgggat ctcgggctcg tgcgctcgct gccggagctt taccggctga
     4501 cgaaagagca gctgatggag ctcgaagggt acggcgagat ctctgcgacg agcgccatcg
     4561 agtcgatcgc ggcgtcgaag caagttccct tcaaccgcgt gctgttcggg ctgaacatcc
     4621 cggacgtcgg ctgggtgacg gcccagaatc ttgcgcgaca cttcgagaac gtcgaccggc
     4681 tgctgtccgc cgcgcaggag gacatcctcg aggtcgaggg catcggcccg gaacgagcgg
     4741 aggcgatcgc ggagtggttc tcggacgagg cgaaccgtgc gctcgtggag gagctgcgtt
     4801 cgctcgggtt gcggttcgag atcggcgacg agctcaagcc ggtcgaaggc ccgctcaccg
     4861 gcaacacctt cgtgatcacc ggcacgttgg agggcttcac gcgcgacgag gcggccgcgg
     4921 cgctggaggc gaaaggcgcg aaggtcgtga actcggtttc cggcaagacg acggggctgg
     4981 tcgtaggcga ggagccgggc aactcgaagc tgacgaaggc ccgcaaggtc gaagtcccga
     5041 tcctatccga agccgacctg gtcgagctcc tgaagcgcac gtaggtgaaa cgaaagtgcg
     5101 ccttctgacg cacatgtcga ctttgtgact acgaaccggc caggacttgt gcgaagcggt
     5161 cctgggcgcg gcgggcccac cagtacgaca ggggatgccc gttctgcacg acggcgaatg
     5221 cgtagcggcc gctgacgtag ccggacagcg acgacgcggt gtccgtcgtg ccggtcttcg
     5281 cgagaacgtt ccctttcgcc ggcggcttct gcagacggtg ttgcagcgtc ccgttcacgc
     5341 ccgccaccgg gagggaggcg agtagggccg cgccgaccgt cggatcggcc caggcgatct
     5401 tcaacaggct gccgagcgca ttcgccgtca ggcggtcgag ccgcgacagg cccgaaccat
     5461 cgacgatgcg gacgcccgtc atcggcacgc ccgcgtcagt cagggcctgc gtgaccacgg
     5521 ccgctcccgc cgcgctcgtc ccgcggccga gctccgtgag ccccagctgc ttcaacagca
     5581 tctcggcggt gaagttgtcg ctctcgcggt ccatgaagcg caacatcgtc cagagcgtcg
     5641 gcgacgacac gtcggcaagc ggttccgacc acgcgtcggc cgttccggtc cgca
//
LOCUS       DASVEU010000081        13132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1419490,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000081 DASVEU010000000
VERSION     DASVEU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419490"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VF225_03130"
     CDS             <1..602
                     /locus_tag="VF225_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX7144276.1"
                     /translation="DDPQGAAAVNVGGTRHVAELAAPLVYYSTDYVFDGRKSEPYVES
                     DPPNPLSAYGRTKLYGEGAAGEQAWIVRSSGLFGPTGTNFVRTMLDLSRTRDEVAVVD
                     DQRTSPTYVGHLAAATRELLERPFGLWHLAADGDATWAEFAEAIFEEAGLETQVRRIS
                     SDELGRPAQRPAYSVLRSEKGAPTLPHWREGLRECLSRL"
     gene            complement(599..1441)
                     /locus_tag="VF225_03135"
     CDS             complement(599..1441)
                     /locus_tag="VF225_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144277.1"
                     /translation="MSVIPGPSSSLRHEAVSFAGVVERNWYLIKRYGWWELAFFFWSL
                     ANTLTIVFIAKGIKAAGGQIDVNRATTTLLIGAVIWSFLGLVFEFMTETVAWERWEGT
                     IEYTFMAPIRRPVHLAGVGIFAVLYGVLRSAILFVVIALMFGLHMPDANFLAAAIILA
                     IASISFMGIGTMTSVLPLISPEKGAQLGFIAQGTLLVISGVYYPVSVLPGWMQWLSTI
                     SPATYALRGIRAAILDGAGITSLWGDIWPLIIIGVIAFPLGVWIFKRGEIYAKRHGKL
                     KRSG"
     gene            complement(1438..2259)
                     /locus_tag="VF225_03140"
     CDS             complement(1438..2259)
                     /locus_tag="VF225_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144278.1"
                     /translation="MFAVEVDNLRKEFFRRDAARTHMRKRRKRVPALEDVTFTMDRGE
                     CVAILGQNGSGKSTLVRLLSTLLLPDGGRATIFGHDVVKDSRAVQKLVNRVSVEASFF
                     KKMSSEENLSYAARFYGMGPTETREKIPKILNRVGFPTDRKHEGMEHLSRGMQQKVAL
                     ARALLTSPVLLLLDEPTTGLDPRSKLEVQDFIREVRRTHDATILLCTHDLQEAEILAD
                     RIGILDRGKLIALDPADELKEQYNAETLEDAFFSATGRSFEEEGEGEDVERGVFA"
     gene            complement(2386..2841)
                     /locus_tag="VF225_03145"
     CDS             complement(2386..2841)
                     /locus_tag="VF225_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144279.1"
                     /translation="MNVWADQWDATDDWSGGGAKSKRLVDRGPLLGASLYELGPGNSV
                     VYHFHHGSEELLIVLRGRPTLRGPEGERRLDEGEVVHFPVGPDGAHGLRNETKEIVRY
                     VVAGIRVSPEVAEYPDLKKITAQARTSSQTGERLWMIHDLEVDSDTPDA"
     gene            3001..4446
                     /gene="glnA"
                     /locus_tag="VF225_03150"
     CDS             3001..4446
                     /gene="glnA"
                     /locus_tag="VF225_03150"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7144280.1"
                     /translation="MSVEETKHYLANGQASAPDAREALEWARETTAQMVDLKFCDLLG
                     TWQHMTLPIRALDESAFDDGLGFDGSSIRGWQGISESDMLLMPDASSAILDPFTEAPT
                     LSLICEIADPITREPYAKDPRRLAKRAEEYLLSTGIADTAFMGPECEFFVFDEVSYEL
                     GPNRSHYSVDSGEGHWNSGKPGLGYTVREKEGYFPPAPHDTLHNLRTRMVLTLERLGI
                     PCEFHHHEVASGGQCEIDLRFQALTRMADQVMTYKYVVKNVAHAAGKSVTFMPKPIFG
                     DNGSGMHTHQSLWKEGTPLMSDKSGYAGLSQLARAYVGGLLAHAPALLAFCAPTTNSY
                     RRLVPGYEAPVNLVYSMRNRSACIRIPMYSDSPKAKRIEFRCPDAAANPYLAFSAMLM
                     AGLDGIQKGLDAGEPADWDLFEEDRGVAQVPGSLSEALDALERDHAFLTAGGVFSEEL
                     IRTWIDYKRENEADVVRLRPHPAEFSLYYDC"
     gene            4459..4995
                     /locus_tag="VF225_03155"
     CDS             4459..4995
                     /locus_tag="VF225_03155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144281.1"
                     /translation="MLELGVRPNDTLADPPSVELRHLEPEDWRAVAEIYWDGMRDGMA
                     TFQTEVPSWEAWDAAHLWGHRLVADLLGEVVGWAALSPASTRRCYVGVVEDSVYVGRE
                     ARGLGIGRALLQELIAGAEATGIWTIQTSIFPENRASLALHERCGFRVVGTRERVAKR
                     DGIWRDTMFLERRSEVVS"
     gene            5108..5458
                     /locus_tag="VF225_03160"
     CDS             5108..5458
                     /locus_tag="VF225_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7144282.1"
                     /translation="MTKVEAVVIRERVETVIDAVEHATGHIGVTVIEAIGHGRQRGVT
                     HEYRGRIFESRFLPKAQLTFIVEDELADAVVEAIVDSARSGNESGDGLVWTSAIENVT
                     HNRTGAKLEKEVEA"
     gene            5459..6868
                     /locus_tag="VF225_03165"
     CDS             5459..6868
                     /locus_tag="VF225_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7144283.1"
                     /translation="MPSKDLLIAANTIWVVIAAILVMFMQAGFAFLEAGLTRMKNAAH
                     IAGKNVLIFAVCSIVYWAVGFGIAFGDGNSFVGTSGFFPSSNELLAIGQAPYSFFTGI
                     PAASGYLFEVVFAGVSLAIVWGAMAERAKLWVYPAFGVIFTLIYSVTSHWIWHAGGWL
                     FGKGMQDFAGSTVVHYQGALAALAGALLLGPRIGKFGRDGRANPIPGHNMPYAVLGTI
                     ILWFGWFGFNPGSTLGVVTGDRIGYFGYVALTTNIAAAAGAIGGIASAWLILRKPDVS
                     MMLNGVLAALVAITAASGFVAPWAAIVIGGVSGWIAVAGVLFVERIGIDDPIGAVAVH
                     GMSGVWGTLATGLFAVPALASNLATGTGGLVYTGSFHQLGVQALGLVAVGGFTFSASF
                     GSLWVMKRVWGIRVEPETETAGLDVSEHGMWGYPEFYIPVPGGYGTETHGHLGLSHGN
                     GHTSTAPAVPAAQPEPAPG"
     gene            6938..7642
                     /locus_tag="VF225_03170"
     CDS             6938..7642
                     /locus_tag="VF225_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7144284.1"
                     /translation="MKYQFVDCRWELGKPERGRELYLGGHIPGASFLDVDTDLSSPPG
                     LRGRHPLPDPEQFASAAIRAGIGDEVFVVAYGNMGGAERLWWLLRHFGHERCAVVDLE
                     SWHGPLIAGTEAIEPAELTLGRRDDDTIEAEELDRRRDDLVVVDARLPPRWRGEPNPI
                     DKVPGRIPGALTAPWNKAPPELPEGELVAYCGSGVTATVLLHRLHLSGRDGKLYPGSW
                     SEWEQLGLPVERDSDD"
     gene            complement(7639..8163)
                     /locus_tag="VF225_03175"
     CDS             complement(7639..8163)
                     /locus_tag="VF225_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7144285.1"
                     /translation="MQQVLVLNASYEPLNVTSVRRAHVLVFKGKAEVIEELDQPLHSA
                     TDTYPWPHVIRLVAYVRVPRAVQRKISRRALFARDSWRCVYCGTTAGRLTLDHVIPRS
                     RGGESIWENVVTACAPCNHHKGNRTLEEARMELVHPPKAPAPVLFIRLAAPKIPNGWQ
                     PYLAHFGGAATSTA"
     gene            complement(8296..8772)
                     /locus_tag="VF225_03180"
     CDS             complement(8296..8772)
                     /locus_tag="VF225_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX7144286.1"
                     /translation="MPLSTAEWIAIGACVAAAVALLVAGLAWWKLRAIRSSQLVLLGG
                     GKQDLVDFAVSLQGRIDDLHRAVDEIAAGLSRVDRRVDTTVSKTSLVRYDAIEGAGGH
                     QSASIALLDAGRSGVILTAIQGRDYARIYVKELDHGRPSVALSPEEEQAVERAMAG"
     gene            complement(8772..9935)
                     /locus_tag="VF225_03185"
     CDS             complement(8772..9935)
                     /locus_tag="VF225_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144287.1"
                     /translation="MTERDSDIEFDFFDEPETEEASQRVRTPRRPPPGGPRRPIRPPQ
                     GVIPMLRLAGLIAFLILAVVLLVVGLRGCASSGKHAKYQGYLQNVRAIAKRSDQIGKD
                     LNTALSATGIKESDLEAKLGSLAAQEQQEVAQAVQLKPPGPLQVEHDHLIEVLKLRAS
                     GLSRLADAFRQTATAKARTSTTSGRLIADQARLLIASDVNWDYYFKEPTRLELQSQNI
                     SDVGSVPDSSIIPNPELASTQAMTQIWLRVHGAATGGSSTTGNHGSALVSVTALPDGK
                     KLSASGAASDNQITASTDLAFQVVVSDSGDFQEFNVGVTLTIQKSPKSIVMRKKIDVI
                     NAGESRTVTFTNIDINGLFGLPTTVKVDVAPVPGEKTTTNNSADYKVIFSLGG"
     gene            10051..11643
                     /locus_tag="VF225_03190"
     CDS             10051..11643
                     /locus_tag="VF225_03190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7144288.1"
                     /translation="MSNAAPALVQSELVLGRYRPLRPLGSGGSGSVWLARDEQNGLDV
                     ALKIVPREGKAASRAEREAEAAARLRHPSCQRAYGFGRDSQHVYIAYEYVPGRTFREA
                     LRGGELNDGSTIEACAQVLEALAHAHSRGIVHRDVKPSNILLAEGRGVSVRVLDFGLA
                     QIQQAETLTAQGDVPGTLAYIAPERLAGGLTTEAADVWAVGLMLWEALAGRHPFWRGS
                     LLETARAIESGAPRLETARPDLPKPLLAAVDGALNLDPSRRPSAAVLAGTLRLVRQKR
                     RRSRGGRGQTIAVPAVVPRLVPAALAAAVAGWAAWALPFFPSGWPLGLAALAAAGTIV
                     DARLGLAFALAVPILPLGNLALGAAVLYAVIALGLLATMWREPERGLLFSLGALLAPI
                     AGVGLFPLAALSIRSPSRRAVQATAAVLTAGIVAGIRGSPLPFDSASPPDLGIAASGD
                     PFTVVSALWGALLSRPALAVMTIVLAAVAVLLPLARARGLWAIAGLGAAFLVAALLLA
                     PGVAVAPLVVVVWATCLAVAVR"
     gene            11669..12445
                     /locus_tag="VF225_03195"
     CDS             11669..12445
                     /locus_tag="VF225_03195"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX7144289.1"
                     /translation="MVLRAIEQKIESLFEGIFGRAFRTNVQPVELARKLAKEMDEHRN
                     ISVSRVYVPNEYTVYLSPDDREQFSSYEDNLRGELQEYLSEHSRREDYALLSPVRVLM
                     ETDADLDVGEFGIATRMVQPDRRKADAEGEPAGEVAPGATMIYKPQAPVPTQAVSAEE
                     LGLEPDLVTLSFDGTRYEVKQRRVVMGRSRDCDIQLSDTNVSRRHAELRQEGASYWIV
                     DLGSTNGLEVNGKRVKRAKLNDGDTITVGSTEVRFSQEHP"
     gene            12442..12933
                     /locus_tag="VF225_03200"
     CDS             12442..12933
                     /locus_tag="VF225_03200"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7144290.1"
                     /translation="MKLIFDSAAVDEVLLVLKIAFLVLLYTFIWRIVRTASRDLRLPQ
                     ESFVLAPSAAAGMQASRPGLHTGRLTVVRSADLETGEDFELNSVQLTIGRGNQNDIAI
                     ATDEYASARHARFEPRQDGVWVQDLGSTNGTYLNGTRLDRPHRLARGDVVRIGETDLR
                     YEQ"
     gene            12930..>13132
                     /locus_tag="VF225_03205"
     CDS             12930..>13132
                     /locus_tag="VF225_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144291.1"
                     /translation="MTGLGHVGVITDTGRKRRRNEDAYVCEPPLFAIADGMGGAQAGE
                     VASRLAAAALKESDARTLGGEER"
BASE COUNT         2079 a         4341 c         4458 g         2254 t
ORIGIN      
        1 aggacgaccc gcagggcgcc gccgccgtca acgtgggcgg gacgcggcac gtcgccgagc
       61 tcgccgcgcc gctcgtctac tactccaccg actacgtttt cgacggccgc aagagcgagc
      121 cgtacgtcga atccgatccg ccgaacccac tctcggcgta cggccgtacg aagctctacg
      181 gcgaaggcgc agccggtgag caggcgtgga tcgtgcgcag ctcggggctc ttcggtccga
      241 ccggcacgaa cttcgtgcgc acgatgctcg acctctcgcg cacgcgtgac gaggtcgccg
      301 ttgtcgacga tcagcgcacc tctccgacct acgtcggcca tctggctgcg gcgacgcgtg
      361 agctactcga gcggccgttc ggcttgtggc acctcgctgc agacggagac gcgacgtggg
      421 ccgagttcgc cgaggcgatc ttcgaggagg ccggcctgga gacccaggtg cgtcgcatct
      481 cgagcgacga gctcgggcgt ccggcgcagc gacccgcgta ctcggtgctg cgcagcgaga
      541 agggcgcgcc gacccttccg cactggcggg agggtctacg cgagtgtctt agccggctct
      601 agccggagcg cttcaacttc ccgtgcctct tcgcgtagat ctcgccccgc ttgaagatcc
      661 aaacgccgag cgggaacgcg atcacgccaa tgatgatcag cggccagatg tcgccccaga
      721 gggacgtgat cccggctcca tccaggatcg cggctcggat gccgcgcagc gcgtacgtcg
      781 ccggtgagat cgtcgacagc cactgcatcc agccgggtag cacggacacc gggtagtaca
      841 cccccgagat cacgagcagc gtcccctgcg cgatgaaacc gagttgtgcg cccttctccg
      901 gtgagatcag cggcagcacg gacgtcatcg tgccgatgcc catgaacgag atcgaggcga
      961 tcgcgaggat gatggccgcc gcgaggaaat tcgcgtcggg catgtgcagg ccgaacatca
     1021 gcgctatgac gacgaacagg attgcgctgc gcagaacgcc gtagaggact gcgaagatgc
     1081 cgacgccggc caggtggacc ggccggcgga tcggcgccat gaacgtgtac tcgatcgtgc
     1141 cctcccagcg ctcccacgcg accgtctccg tcatgaactc gaagacgagg ccgaggaagg
     1201 accagatgac cgcgccgatg agcagcgtcg tcgtcgcgcg gttgacgtcg atctggccgc
     1261 ctgccgcctt gatccccttc gcgatgaaga cgatcgtgag cgtgtttgcg agcgaccaga
     1321 agaagaacgc gagctcccac cagccgtagc gcttgatcag gtaccagttc cgctcgacca
     1381 cgccggcgaa cgacaccgct tcgtggcgga gcgacgaact cggaccgggg atcacgctca
     1441 tgcgaacaca ccccgttcca cgtcctcgcc ttcgccttcc tcttcgaaag agcgcccggt
     1501 tgccgagaag aaggcatcct cgagcgtttc cgcgttgtac tgctccttca actcgtcggc
     1561 agggtcgagc gcgatcagct tgccgcggtc gaggatgccg atgcggtcgg ccaggatctc
     1621 ggcctcctgg agatcgtgcg tgcagaggag gatcgtggcg tcgtgcgttc tccgcacctc
     1681 acggatgaag tcctggacct cgagcttgga ccgcggatcg agacccgtgg tcggctcgtc
     1741 cagcagcagc agcaccggtg aggtgagcag cgcacgtgcg agcgcaacct tctgctgcat
     1801 tcctcgtgaa aggtgctcca tgccctcgtg ctttcgatcg gtcggaaagc cgacgcggtt
     1861 caggatcttc gggatcttct cccgagtctc ggtcgggccc atgccgtaga acctcgcggc
     1921 gtacgagagg ttctcctccg acgacatctt cttgaagaac gacgcttcga cggacacgcg
     1981 gttgaccagt ttctgcactg cacgcgagtc cttcacgacg tcgtggccga agatcgttgc
     2041 tctgccgccg tctggcagca gcagcgtcga gagcagccgg accagcgtcg acttgcccga
     2101 gccgttctgc ccgaggatcg cgacgcactc gccgcggtcc atcgtgaacg tgacgtcttc
     2161 gagcgccggc acgcgcttac gccttttgcg catgtgcgtt cgcgccgcat cgcggcggaa
     2221 gaactccttg cgcaagttgt cgacttctac tgcgaacact tcattgctcc tacgattcgg
     2281 cgggacacaa gtacgcgcaa gacacctgct gaacgcaggc ggcgcacggg acgtacgaag
     2341 tcagccgaag aagagcatca tacgctcaag acggtagcaa acgtgtcaag cgtctggtgt
     2401 atccgaatcg acttcgaggt cgtgaatcat ccagagtcgc tcgcctgttt gcgagctcgt
     2461 gcgtgcctgc gccgtgatct tcttgaggtc cgggtattcc gcgacttcgg gggagacacg
     2521 aatgcccgcc acgacatagc gcacgatctc cttggtctcg ttgcgcagtc cgtgcgcacc
     2581 gtcgggccca accggaaagt gcacgacctc gccctcgtcg agccgccgct caccctcggg
     2641 accgcgcagc gtcggccgtc cccggagcac gatcaggagc tcttcggagc cgtggtgaaa
     2701 gtggtagacg acggagttcc ccggaccgag ctcgtacagg ctcgccccga ggagcggacc
     2761 gcggtcgacg agccgcttgc tcttcgcgcc gccgcccgac cagtcgtcgg ttgcgtccca
     2821 ctggtcggcc cagacgttca tcggccgatc ctggcatacg gcgcttgacc gcagtccggt
     2881 cgccccgcta aggtgctgtc caggtcgctc gccgccgaag gcgagcgccg aggcaacgga
     2941 gcctcaggac tcagagagtc ctgggctctt tttttgtggt cccgagttgg aaggagcggc
     3001 atgtctgtcg aagaaacgaa gcattacctg gcgaacgggc aggcgagcgc gcccgatgcc
     3061 cgcgaagccc tcgagtgggc gcgcgagacc acggcccaaa tggtcgacct gaagttctgc
     3121 gacctgctcg ggacgtggca gcacatgacg ctgccgatca gggctctcga cgagtccgcc
     3181 ttcgacgacg gcctcggctt cgacggctcg tcgattcgcg gctggcaggg gatctccgag
     3241 tcggacatgc tgctgatgcc ggacgcctca tccgcgatcc tcgatccatt caccgaggcg
     3301 ccgacgctgt cactgatctg cgagatcgcc gatccgatca cgcgcgagcc gtacgcgaag
     3361 gatccacgcc ggctcgcgaa gcgcgcagag gagtacctgc tctcgacggg gatcgccgac
     3421 acggccttca tgggcccgga gtgcgagttc ttcgtgttcg acgaggtctc gtacgagctc
     3481 ggcccgaatc gctcgcacta ctccgtggac tcgggcgagg gccactggaa ctccggcaag
     3541 ccggggctcg gctacaccgt tcgcgagaag gagggctact tcccacccgc cccgcacgac
     3601 acgctccaca acctgcgcac gcgcatggtg ctgacgctgg aacgcctcgg catcccttgc
     3661 gagttccatc atcacgaggt cgcgtccggc ggccagtgcg agatcgacct tcgcttccag
     3721 gccctgactc gcatggccga tcaggtcatg acctacaagt acgtcgtcaa gaacgtcgcg
     3781 catgcggccg gcaagtcggt gacgttcatg ccgaagccga tcttcggcga caacggctcg
     3841 ggcatgcaca cgcaccagtc cctctggaag gagggcacgc cgctgatgtc cgacaagtcg
     3901 ggctacgcag gcctgtcgca gctggcccgt gcctatgtcg gcggcctgct cgctcatgcg
     3961 cctgccctgc ttgcgttctg cgcgccgacg acgaactcgt accgccgcct ggtgccgggc
     4021 tacgaagcgc cggtcaacct cgtgtactcg atgcggaacc gcagtgcctg catccggatt
     4081 ccgatgtact cggactcgcc caaggcgaag cggatcgagt tccgctgccc cgatgcggct
     4141 gcgaacccgt acctcgcgtt ctcggcgatg ctgatggctg ggctcgacgg catccagaaa
     4201 gggctggacg ccggcgagcc ggcggactgg gacctcttcg aagaggaccg cggcgttgct
     4261 caggtcccgg gctcgctctc cgaggccctc gacgcgctcg agcgggatca cgcgtttctc
     4321 acggcaggcg gcgtgttcag cgaggagctg atccgcacct ggatcgacta caagcgcgag
     4381 aacgaggccg acgtcgtgcg tctgcggccc catcccgcgg agttctccct ctactacgac
     4441 tgctgaaagg aggcgaacgt gttggagctc ggtgtacggc caaacgacac gcttgcagat
     4501 ccgccgtccg ttgagctccg gcacctcgag ccggaggact ggcgggctgt tgcagagatc
     4561 tactgggacg ggatgcgcga cgggatggcg acgttccaga cggaggttcc ctcctgggaa
     4621 gcctgggacg cggctcacct ctggggccac cggcttgtgg ccgacctgct cggcgaggtc
     4681 gtggggtggg cggccctgtc gcccgcatcg acgcggcggt gctacgtcgg cgtcgtcgag
     4741 gacagcgtct acgtcgggcg tgaagcgcgc ggcctcggca tcggccgggc actgctgcag
     4801 gagctgatcg cgggcgccga ggccaccggg atctggacga tccagacgtc gatcttcccg
     4861 gagaatcgtg cctcgctcgc gctgcacgag cgttgcgggt tccgcgtcgt cgggacgcgc
     4921 gagcgcgttg cgaagcgcga cggaatctgg cgggacacga tgttcctcga gcgaagaagc
     4981 gaggtggtct catgactttg tggctcagag ccactaagtg gttttcgggt ggtcgcttcc
     5041 ctgtgggtga gccgacacac gccaagtctc gtgtgcttgt ggctcagagc cacaaggggt
     5101 ggtcggcatg accaaggtcg aggcagtcgt gatccgcgag cgcgtggaga cggtgatcga
     5161 cgcggtcgag catgcgacgg gtcacatcgg tgtcacggtg atcgaggcga tcgggcacgg
     5221 acgccagcgt ggcgtcacgc acgagtaccg cggtcgcatc ttcgaatcgc ggttccttcc
     5281 gaaggcgcag ctcacgttca tcgtcgaaga cgagctcgcc gacgcagtcg tcgaggcgat
     5341 cgtggactcg gcgcgcagcg gcaacgaatc cggcgacggc ctcgtgtgga cgtcggcgat
     5401 cgagaacgtc acgcacaacc gaacgggcgc gaagctcgag aaggaggtgg aagcctaaat
     5461 gcctagtaag gatctactga ttgcggcgaa cacgatctgg gtcgtcatcg cggcgatcct
     5521 cgtgatgttc atgcaggcgg gctttgcctt cctcgaggcc gggctgacgc gaatgaagaa
     5581 cgcggcgcac atcgccggca agaacgtcct catcttcgcg gtctgctcga tcgtctactg
     5641 ggcggtcggc ttcgggatcg ctttcggaga cggcaacagc tttgtcggga cgagcggctt
     5701 cttcccttcc agcaatgagc tgctcgcgat cggccaggcg ccgtactcgt tcttcaccgg
     5761 catcccggcg gccagcggct atctcttcga ggtcgtcttc gcgggcgtct cgctcgcgat
     5821 cgtctggggt gcaatggccg agcgggcgaa gctctgggtc taccccgcgt tcggcgtcat
     5881 cttcacgctc atctactcgg tgacgtcgca ctggatctgg cacgccggcg gctggctgtt
     5941 cggcaagggc atgcaggact tcgccggctc gacggtcgtg cactaccagg gcgcgctcgc
     6001 cgccctcgcg ggtgcgctgc tgctcgggcc gcggatcggg aagttcggcc gtgacggccg
     6061 cgcgaacccg atcccgggtc acaacatgcc gtacgcggtg ctcgggacga tcatcctctg
     6121 gttcggttgg ttcggattca acccaggctc gacactcggt gtcgtcactg gcgatcgcat
     6181 cggctacttc ggctatgtcg cgttgacgac caacatcgcg gctgccgccg gcgcgatcgg
     6241 cggcatcgcg agcgcgtggt tgatcctgcg caagcccgac gtgtcgatga tgctgaacgg
     6301 cgtcctcgct gcgctggtcg cgatcaccgc cgcctcgggg ttcgtggccc cgtgggctgc
     6361 gatcgtgatc ggcggggtat ccgggtggat cgccgttgca ggcgtcttgt tcgtcgagcg
     6421 gatcggcatc gacgatccca tcggcgccgt cgccgtgcac gggatgtcag gcgtgtgggg
     6481 gacactcgcg accggcttgt tcgcggtgcc cgcgctggca tcgaatctcg ccaccggcac
     6541 gggcgggctc gtctacacgg gctccttcca tcagctcggc gtgcaggcgc tcggcctcgt
     6601 cgcagtcggc gggttcacgt tctccgcctc attcggatcg ctctgggtga tgaagcgggt
     6661 gtggggaatc cgtgtcgagc cggagactga gacggcaggt ctcgatgtct cggaacacgg
     6721 catgtggggc taccccgagt tctacatccc tgtccccggt ggctacggca ccgagacgca
     6781 cggccatctc ggcctctccc acgggaacgg gcacacgtcg actgctcccg cggtgcccgc
     6841 cgctcagccc gaaccggcgc ccggctagcg accgcttcct cgagggcgcc gcgaactcag
     6901 cggcgccctc gagctttccc ggtaaagatg cggcgagatg aagtatcagt tcgtcgactg
     6961 ccgctgggag ctcggcaaac cggagcgcgg ccgcgagctc tacctcggcg gccacatccc
     7021 cggcgcgagc tttctcgatg tggacaccga tctgtcgtca ccgccgggtc ttcgcggcag
     7081 acatccgctg ccggatccag aacagttcgc gagcgcggcg atccgagcag gtatcggcga
     7141 cgaggtgttc gtcgtcgcgt acgggaacat gggtggcgcc gaacggctct ggtggctgct
     7201 gcgtcacttc ggccacgaac gctgcgcggt ggtcgacctc gagtcttggc acggaccact
     7261 catcgcaggc acggaagcaa tcgagccggc ggagctgacg ttgggtcggc gcgacgacga
     7321 cacgatcgag gccgaggagc tcgaccgccg ccgggacgac ctggtcgtcg tggatgcgcg
     7381 tctcccgccc cgctggcgcg gcgagcccaa tccgatcgac aaggtgcccg gccggatccc
     7441 aggtgcgctc accgcgccgt ggaacaaagc gccgcctgag ctgccggagg gtgagctcgt
     7501 cgcctactgc gggtcaggcg tgaccgcaac cgtgctgctg caccgcctcc acctcagcgg
     7561 tcgcgacggc aagctctacc cggggtcctg gagcgagtgg gagcagctcg gcctgccggt
     7621 cgaacgcgac tcggacgact aagccgtgga ggttgccgcg ccgccgaagt gagcgagata
     7681 gggctgccaa ccgttcggga tcttcggtgc ggcaaggcgg atgaacagca ccggggcagg
     7741 agccttcggc ggatgaacga gctccatgcg cgcctcctcc agcgtgcggt tccccttgtg
     7801 gtggttgcac ggcgcacacg ccgttacgac gttctcccag atcgattccc caccgcgcga
     7861 tctcgggatg acgtggtcga gcgtcagccg gcccgcggtg gtcccgcagt agacgcaacg
     7921 ccaactgtcg cgcgcgaaga gcgcgcgccg ggagatcttg cgctggacgg cccgcggcac
     7981 gcgcacgtag gccacgagcc ggatcacgtg tggccacggg tacgtgtcgg tcgctgagtg
     8041 cagcggctgg tcgagctcct cgatcacttc ggccttcccc ttgaacacaa gcacgtgggc
     8101 acgccgcacg gatgtgacat tcagcggctc gtaactcgcg ttcaaaacca gaacctgctg
     8161 catctaggcg gaaatgtagc tgagcggact ccggtcgtac gcgaagagag gtcggtcggg
     8221 tcgactggtc atttccgacg gacgcacgtg cagagcacgt gcgtcttggt ggaggcaccg
     8281 caagcggcgc ctcggtcacc cggccatggc ccgttcgacg gcctgttcct cctccggaga
     8341 cagcgccacg gacggcctgc cgtggtcgag ctccttgaca tagatcctgg cgtagtcgcg
     8401 gccctgaatc gcggtcagga tcaccccgct gcggccggcg tccagcaggg cgatcgaggc
     8461 cgactggtgc ccaccggcgc cctcgatggc gtcgtagcgg acgagcgagg tcttggagac
     8521 cgtcgtgtcg acgcggcgat cgacccgcga caggcccgca gcgatctcgt ccacggctcg
     8581 atgcaggtcg tcgattcgtc cctggagcga gactgcgaag tcgaccaggt cctgcttccc
     8641 tcctccgagc agcacgagct gggacgaccg gatggcgcgc agcttccacc aggcgagacc
     8701 ggccacgagc agcgcgactg ccgcagcgac gcacgccccg atcgcgatcc actccgcggt
     8761 cgagagaggc actaacctcc tagggagaag atgactttgt agtcggccga gttgttcgtc
     8821 gtcgtcttct cgcctggcac cggcgcgaca tcgaccttga ccgtcgtcgg cagcccgaac
     8881 agcccgttga tgtcgatgtt cgtgaacgtg accgtcctcg actcgccggc gttgatcaca
     8941 tcgatcttct tgcgcatcac gatcgacttc ggtgacttct ggatggtcag cgtcacgccg
     9001 acgttgaact cctggaagtc acccgagtcc gacaccacca cctggaacgc aaggtcggtc
     9061 gacgcggtga tctggttgtc gctcgccgcg ccgcttgcac tgagcttctt gccgtcggga
     9121 agcgccgtca ctgagacgag cgcgctgccg tggtttccgg ttgtcgacga cccgcctgtc
     9181 gccgcgccgt ggacgcgcag ccaaatctgc gtcatcgcct gggtgctcgc gagctcgggg
     9241 ttcggaatga tgctggagtc ggggacgctg ccgacgtcgc tgatgttctg gctctgcagc
     9301 tcgagccgag tcggctcctt gaagtagtag tcccagttga cgtcgctcgc gatcaggagg
     9361 cgcgcctgat ccgcaataag ccggccggag gtcgtcgagg tgcgcgcctt cgccgtcgca
     9421 gtctggcgga atgcatcggc cagccggctg agcccgcttg cgcgaagctt gagcacctcg
     9481 atgaggtggt catgctcgac ctgaagcggg ccgggcggct tcagctggac cgcctgcgcg
     9541 acctcttgct gctcctgcgc agcaagactg ccgagcttcg cctcgagatc ggactccttg
     9601 atgccggtcg cgctgagcgc cgtattcaga tccttgccga tctggtcgga gcgcttcgcg
     9661 atggcgcgga cgttctggag gtatccctgg tacttggcgt gcttcccgct ggacgcgcag
     9721 ccccgcagtc cgacgacgag caggacgacg gcgaggatga gaaaggcgat cagcccggcg
     9781 aggcggagca tcgggatcac gccctgcggt ggccggatcg gccggcgggg cccgcccggc
     9841 ggtgggcgcc ggggggtgcg cacgcgctgc gaagcctctt cggtctccgg ctcgtcgaag
     9901 aagtcgaatt cgatgtctga gtcgcgctcg gtcatgggaa aggggcggcc gggagagtcc
     9961 cagccgaagc ggcgttcact ctaacccgta cctcgtccgt tctcgacggg tgaaggactt
    10021 gttcttgccc ccgccaaccc tggagagccg gtgtccaacg cagccccagc ccttgtccag
    10081 agcgagctcg tcctcgggcg ctaccgcccg ctgaggccgc tcgggagcgg cggttctggc
    10141 tccgtctggc tagccaggga cgagcagaac gggctcgacg tcgcactcaa gatcgtgccc
    10201 cgtgagggga aggcggcatc ccgcgccgag cgcgaggccg aggctgccgc ccgtctgcgc
    10261 catccctcct gtcagcgcgc ctatggcttc ggccgtgact cccagcatgt ctacatcgcc
    10321 tacgagtacg tccccgggcg gacctttcgc gaggcgctcc gcggcggcga gctgaacgat
    10381 ggctccacga tcgaagcgtg cgcccaggtg ctcgaggcac ttgcgcacgc gcactcgcgc
    10441 gggatcgttc accgcgacgt caagccgtcg aatattctgc tcgcagaagg gagaggtgtg
    10501 tcggtgcgtg tgctcgactt cggcctggct cagatccagc aggccgagac cctgaccgcg
    10561 caaggcgacg tgccgggcac gcttgcctac atcgccccgg aacgcctcgc cggggggttg
    10621 acgacagagg cggcagacgt gtgggcggtc ggactcatgc tctgggaagc gctggcgggc
    10681 cggcacccgt tctggcgcgg ttctctgctc gagacggcgc gggcaattga gtcgggggcc
    10741 ccgcgcctgg agacggcgcg gccggatctg ccgaagcctc tgctggccgc ggtcgacggc
    10801 gccttgaatc tcgatccctc ccggcggccg agcgcggctg tactggccgg aaccctccgc
    10861 ctcgtacggc aaaagcgccg gcgctcgcgt ggtggccgtg gccagaccat cgcagtgcct
    10921 gcagtcgtgc cacggcttgt ccccgcagcg ctcgcggcgg ctgtcgcagg ctgggctgcg
    10981 tgggcactcc cgttcttccc ttcagggtgg ccgctcggtc tcgcagcgct cgccgctgcc
    11041 ggcacgatcg tcgacgctcg actcggcctc gcgttcgcgc tggccgtgcc catcctgccg
    11101 ctcgggaacc ttgccctcgg tgcggcagtc ctctacgccg tgatcgcgct cgggctcctg
    11161 gcaacgatgt ggcgtgagcc ggagcgcggt ctcctcttct cgcttggggc actcctggcg
    11221 ccgatcgcag gcgtcggcct cttcccactg gccgcactgt ccatacgctc cccgtcacgg
    11281 cgggcggtgc aggccacagc ggccgtgctg accgccggga tcgtcgcggg gatccgaggt
    11341 tcgccgctgc cgttcgacag cgcctctcca ccggacctcg ggatcgccgc gagcggggac
    11401 ccgttcactg tcgtgtcggc cctgtggggg gcgctgctct ctaggcccgc tcttgccgtg
    11461 atgacgatcg tcctcgcggc ggtagcggtc ctgttgcccc tggcgcgggc gcgggggctc
    11521 tgggcgatcg ccgggctggg agccgcgttc ctggtcgcgg cgttgctcct ggcgccgggg
    11581 gtggcggttg ccccgctcgt ggtagtcgtg tgggccacct gcctggcggt agcggtgcgt
    11641 tagcatcgct tgccgggtat tcccggacat ggtcctccgc gcaatcgaac aaaaaatcga
    11701 atcccttttc gaggggattt tcggccgcgc gttccggacg aatgtccaac cggtcgagct
    11761 tgcgcggaag ctcgcgaagg agatggacga gcaccgaaac atctcggtct ctcgtgtcta
    11821 cgtgccgaac gagtacacgg tgtacctctc tccggacgat cgcgagcagt tctcgagcta
    11881 cgaagacaat ttgaggggcg agctccagga gtacctctcc gagcactccc gccgcgagga
    11941 ttacgcgctc ctttcgccgg tccgcgtcct catggagacg gatgccgatc tcgacgtggg
    12001 tgaattcggc atcgcgaccc ggatggtgca gccggaccgg cgcaaggccg acgccgaagg
    12061 cgagccggca ggagaggtgg cacccggcgc gacgatgatc tacaagccgc aggctccggt
    12121 tccgacgcag gccgtcagcg ccgaggagct gggcctcgag ccggacctgg tgacgctgag
    12181 cttcgacggc acgcgctacg aggtgaagca gcgtcgcgtc gtaatgggac ggtcgcgcga
    12241 ctgcgacatc cagctgtcgg acacgaacgt ctcgcggcgg catgccgagc tgcgtcagga
    12301 gggagcgtcc tactggatcg tcgacctcgg ctcgacgaat gggctcgagg tgaacgggaa
    12361 gcgggtgaag cgtgccaagc tcaacgacgg cgacacgatc acggtcgggt cgaccgaggt
    12421 gaggttcagc caggagcacc cgtgaagttg atcttcgact cggctgcggt cgacgaagtt
    12481 ctactcgtcc tcaagatcgc tttcctcgtc ctgctctaca cgttcatctg gcgaatcgtg
    12541 cggactgcga gccgcgatct gcgccttccc caggagagct tcgtgctcgc tccgtccgcg
    12601 gccgcgggca tgcaggcctc gcggcccggg ctgcacaccg ggcgtctcac cgtcgtgagg
    12661 agcgccgacc ttgagaccgg tgaggacttc gagctgaact ccgtgcagct gacgatcggg
    12721 cgcgggaatc aaaacgacat tgcgatcgcc acggacgaat acgcctccgc tcgacacgcg
    12781 cgcttcgagc cgcgccagga cggcgtctgg gttcaggacc tgggctcgac gaacgggaca
    12841 tatctcaacg gcacacggct cgaccggcct caccggctgg ctcgcgggga cgtcgtccgg
    12901 atcggcgaga cggatctcag gtacgagcag tgacgggtct cggccatgtc ggggtgatca
    12961 cggataccgg gcgcaagcgc cggcggaacg aagacgccta cgtctgcgag ccgccgctct
    13021 ttgcgatcgc cgacggtatg ggcggcgccc aggcgggaga ggtcgcatcg cggctcgcgg
    13081 cggcggcgct caaggagagc gacgcaagga ccctgggcgg cgaggagcgc at
//
LOCUS       DASVEU010000082         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1420225,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000082 DASVEU010000000
VERSION     DASVEU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420225"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1108)
                     /locus_tag="VF225_03210"
     CDS             complement(182..1108)
                     /locus_tag="VF225_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7144292.1"
                     /translation="MIVDDHTICGSGWHVFAEVRDFLSFLAMIDDLGVRAGLDGRVVR
                     HTAGDVRPRAHRHAELELNLVVRGTASYLLGDRRYALTPGTLTWLFPGQDHVLVDESA
                     DHELWWAVFRPALVARIATTARARPLGEDDPVGQFSRRLDSGRVRRLGAVFHEVHDAE
                     TVDDVLVNTGLAYLLSFAWHAFLDSDDIVEGVDLHPAVERVARTLRADPNAGDLATLA
                     RAASLSPSHLSRIFKEQTGVSITRFRNQQRLQRFLRLYGRGRRTTALAAALEAGFGSY
                     AQFHRVFREHAGRTPSALRTAADDDLTMGDYL"
     gene            1120..1872
                     /locus_tag="VF225_03215"
     CDS             1120..1872
                     /locus_tag="VF225_03215"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7144293.1"
                     /translation="MTVSTPLVTSEHARQFREEGFFVLRDIVPPGDLDTLRGECQRVI
                     EAREREMDRLGVDKLDLDHRGRRYFLHAYKKSPAVERFLCSDLMAQIAQAALGDTVYL
                     FNEQYVVKAAEKGMQFSWHQDSGFIDYAHRPYLTCWIALDEMTEANGTVYLLPYSRAA
                     TRDVVKHVRDKESNDLVGYFGDDPGYAVSVPARSIACFSSTLFHRSGPNTTDRMRRVY
                     VAQYSAEPILSEDGSQPRRLAEPLLVDGKRVT"
     gene            2064..>2260
                     /locus_tag="VF225_03220"
     CDS             2064..>2260
                     /locus_tag="VF225_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144294.1"
                     /translation="MNSGWVIPPPRQPIKGDEGLRREREAKIGELGRRGLLRSERLRR
                     AMLTVRREDFIPSRYRDHAYE"
BASE COUNT          407 a          743 c          770 g          340 t
ORIGIN      
        1 cgatttgcgg aagcgacgac ctcgtcacgc gagacgcctg cccgtcgtcc aaagcagcag
       61 tcgggactgg tgctggtgct gctcgcacgc gaaagcgcta gcgggtcccg acggagagtg
      121 tgcacttcgt ccgagctggg agcagcagtc cgcgggctgc gtcaggaggc gccgcgcgcg
      181 ctcagagata gtcccccatg gtcagatcat cgtcggcggc ggtgcgcaac gcggacggag
      241 tccgtcccgc gtgctcgcgg aagacgcggt ggaactgggc ataactgccg aagccggcct
      301 cgagggccgc ggcaagcgcc gtggtgcgcc gccctctgcc gtagagacgc aggaaacgct
      361 gaagtcgctg ctggttgcgg aaccgggtga tcgacacgcc ggtctgttcc ttgaagatcc
      421 ggctcaggtg cgaggggctt aggctcgcgg ctcgtgccag cgtggcgagg tcgcctgcgt
      481 ttgggtcggc tcgcagtgtg cgagcgaccc gctcgaccgc cggatgcaga tcgacgcctt
      541 cgacgatgtc gtcgctgtcg agaaacgcgt gccaggcgaa gctcagcaga tacgccaagc
      601 cggtgttgac aagaacgtcg tccaccgtct ccgcatcgtg cacttcgtgg aagacggcgc
      661 cgagacggcg aactctgccg gagtcgagcc ggcgcgagaa ctgcccgacg ggatcgtcct
      721 cgcccagggg tcgcgctcgt gcggtagtcg cgatccgagc gacgagagcg ggccggaaga
      781 ccgcccacca gagttcgtgg tcggcggact cgtcgaccag gacgtgatcc tgaccgggaa
      841 acagccaggt cagcgtgccg ggcgtgagcg catagcgccg atcgcccagc aggtaggagg
      901 cggtgccgcg gacgacgagg ttgagctcga gctcggcatg ccgatgggcg cggggtcgga
      961 cgtcgccggc cgtgtgccga accacgcgtc cgtccaggcc agcccgaact ccgaggtcgt
     1021 cgatcatcgc aagaaacgat aagaagtcgc gcacttccgc gaagacatgc cagccggaac
     1081 cgcagatcgt gtgatcgtca acgatcaccg gaggacaaca tgaccgtctc aactccgctc
     1141 gtcacgtccg agcacgcgcg tcagttccgc gaggagggct tctttgtcct gagggacatc
     1201 gtgcccccgg gcgatctcga cacgctgcgc ggcgagtgcc agcgtgtcat cgaagcgcgc
     1261 gagcgggaga tggatcgcct cggcgtcgac aagctcgacc tcgaccaccg cggcaggcgc
     1321 tacttcctcc acgcatacaa gaagagcccc gcagtcgagc gctttctgtg ctcagacctc
     1381 atggcccaga tcgcccaggc ggcgctcggc gacaccgttt acctgttcaa cgagcagtac
     1441 gtcgtgaagg ccgccgagaa gggaatgcag ttcagctggc accaggactc gggcttcatc
     1501 gactacgcgc accggccata tctcacctgc tggatcgcgc tcgacgagat gacagaagcg
     1561 aacggcaccg tgtatttgct gccctactcg cgcgccgcca cgcgagacgt cgtgaagcac
     1621 gtccgcgaca aggagagcaa cgacctggtc ggctacttcg gggacgaccc gggctacgcg
     1681 gtgagcgtgc cggccaggag catcgcctgc ttctcgtcga ccctttttca ccgcagcggc
     1741 cccaatacga cggaccgcat gcgtcgcgtc tacgtggcgc aatattcggc cgagccgatc
     1801 ctcagcgagg atggctcaca accgcggagg ctcgccgaac ctctcctcgt cgacggcaag
     1861 cgcgtgacgt gatccgggcg ccccgcgacc ctggtacgcc gtggccactt gcgctttgcg
     1921 cgcggaccaa cagcgccgtc taagccgtcc cggcgctgcc gcgtcttgcg caaacgggga
     1981 gcgggcgagg ggcacatttc cactgggagc ctcgatcgag gacgacgaaa tccgtgccgc
     2041 actcggccta agctaaaaag ccgatgaaca gcgggtgggt cattccgccg ccgcgccagc
     2101 cgatcaaggg cgacgagggc ctgcggcggg agcgggaagc caagatcggg gagctcgggc
     2161 gacgcgggct ccttcgctcc gagcggctcc gcagggcgat gctcaccgtg cgtcgcgagg
     2221 acttcatccc gtctcgctat cgggaccacg cctacgaaga
//
LOCUS       DASVEU010000083         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_256056,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000083 DASVEU010000000
VERSION     DASVEU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256056"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /gene="pilM"
                     /locus_tag="VF225_03225"
     CDS             <1..1804
                     /gene="pilM"
                     /locus_tag="VF225_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX7144295.1"
                     /translation="EDLSAAALEPAAEEDVWKKEVSFSRKPEAEPVAEQPSDDPPAAE
                     EEPIPAEQAVAEEPITVEEEVADEPAPTETPASLEVEAEPWNAPPSLPPAPIAEAATA
                     IVPVPTPSVDVHEPVTAADLPPLAADAAKVPFWKKELSFGGKRRAKPDEAPTAIVPVP
                     TPSVDVHPPVAAADLPPLADDAAKAPFWKKELSLGGRKRDKPVKERKQKAPKVKAERA
                     PKTKEPKQPKEPKERKHKSPKVKVEGGSSKGRIIGLKIGASQLAAARVSNNGSAELLQ
                     VAREPLAAGIVVGGELRDPDALAEALKDFFTKHGLPKKGVRLGIANNRIGVRTFEIAG
                     IEDQKQLANAIRFRAQETLPIPIDEAVLDYQVLGESVGEDGQSIKRVLLVVAYRELID
                     RYVDACKKAGITLAGIDLEAFALLRALQAPQDGVGTDASAALVVVAIGHERSTFAVSD
                     GRVCEFTRVLEWGGSALNVAIARAIDAAPSEVEGVKRALALTDEMVPDGLTSDQAKNA
                     REAMRRAIQTFARELVSSLQYYQNQPGSLGIGEIVLTGGTAHLPGISGELERLIGVHV
                     RVGDPLARMKVSKKVGQPEQIGSLAVAIGLGIED"
     gene            1809..2423
                     /locus_tag="VF225_03230"
     CDS             1809..2423
                     /locus_tag="VF225_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7144296.1"
                     /translation="MRAVNLLPRDQNQRTIKKESLPVLIGACSAVLVAAVLGAMFMMG
                     SGKVAAQQRKLDDLNRQYQALPPPPAGPSVAQQQLAGEQSARVSALSTALTSRVAWDR
                     VFREFSLVLPDDVWLTTLTAKSPISPLTNVSTSSPGGAPSQFTIQGRTYSHDGVARLL
                     SRLQVVPDLTNVQLQSSTLTKVGGQNVVDFSIAADIKVSGAPGA"
     gene            2420..3136
                     /gene="pilO"
                     /locus_tag="VF225_03235"
     CDS             2420..3136
                     /gene="pilO"
                     /locus_tag="VF225_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7144297.1"
                     /translation="MKIDMDTIMKRIKALSPGALIGIMVGAVLVFGLAGWFLLVRPQG
                     AKVTSLKTQATEVQAKIDAYHQQVAAARAAPKIEVADVYRLAKAMPDRTDMPDLLLEL
                     SQLARDTGIRFDSISPQTIAPIASYTVLPISVTFQGNFYNLADFLYRLRSLVSVHAGR
                     LDATGRLFSVDTLTFNESTLKFPQIQATLVIDAFVYGTGVPATAPIPTTPTTTTTTTA
                     ATTTTTTETPPSGASATGAP"
     gene            3136..>3795
                     /locus_tag="VF225_03240"
     CDS             3136..>3795
                     /locus_tag="VF225_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144298.1"
                     /translation="MAKKHVDPLKAKQKKQKIIAAVLGVAFLGLMAFQVPRVMKMMKT
                     PPPPVQPTAATATTPAGAPTLAAPTLGGAQQPGPATDSTGSLAAAPATTVQDGQLASF
                     SRFVSKDPFSQQLSDTPSPSSSTGSSGSSSSGGGAGPRAPAVHGNAPAPGSAVISVNG
                     TLYTVATKTDFPQPTPVFHLVSVTAHTAKISIAGGSYSSGAQTVTLRENKPVTLMNTA
                     DG"
BASE COUNT          686 a         1327 c         1214 g          568 t
ORIGIN      
        1 cgaggatctc tctgctgccg ccctcgagcc ggcggccgag gaggacgtct ggaagaagga
       61 ggtttcgttc agccgcaagc cggaggccga gcccgtcgcc gagcagccct cggacgatcc
      121 gcccgctgcc gaagaggagc ccatccccgc tgaacaagcc gtcgccgaag agcccatcac
      181 ggtcgaagag gaagtcgccg acgagccggc gcccacggag accccggcga gcctcgaggt
      241 cgaagccgaa ccctggaatg ccccgccgtc attgccgccg gcacctatcg ccgaggctgc
      301 gaccgcgatc gtccccgtgc cgactccgtc ggtcgacgtc cacgaacccg tcaccgcagc
      361 cgaccttcct cccctcgccg ctgacgcggc aaaggtgccg ttctggaaga aggagctctc
      421 attcggcggc aagaggcgcg ccaagcccga cgaagctccg acggcgatcg tccccgtgcc
      481 gactccgtcg gtcgacgtcc acccacccgt cgccgcagcc gacctgcctc ccctcgccga
      541 cgacgctgca aaggcgccgt tctggaagaa ggagctctcg ctcggcggca ggaagcgtga
      601 caagcccgtc aaggagcgca agcagaaggc cccgaaggtg aaggccgagc gcgccccgaa
      661 gacaaaagag cccaagcagc cgaaagagcc caaggagcgt aaacacaagt ctccgaaggt
      721 gaaggtcgag ggcggcagca gcaagggccg catcatcggc ttgaagatcg gcgcgtcgca
      781 gctggctgcg gcgcgtgtct cgaacaacgg ctcggcagag ctgctccagg tggcgcgcga
      841 gcctctcgcg gcgggcatcg tcgtcggggg agagctgcgc gatcccgacg cgctcgccga
      901 ggcgctcaaa gatttcttca cgaagcatgg cttgcccaag aagggcgttc ggctcgggat
      961 cgccaacaac aggatcggcg tccgtacctt cgagatcgcc ggcatcgagg accagaagca
     1021 gctcgccaac gcgatccgtt tccgcgcaca ggaaacgctg ccgatcccga tcgacgaggc
     1081 tgtgctcgat taccaggtgc tgggcgaaag cgtcggcgag gacggccagt cgatcaagcg
     1141 cgtcctgcta gtggttgcgt accgcgaact gatcgaccgt tacgtcgatg cctgcaagaa
     1201 ggcgggcatc accctcgccg ggatcgacct ggaggcgttc gcgctcttgc gtgcgctgca
     1261 ggcgccgcag gacggcgtcg gtaccgatgc gtcggccgct ctcgtcgtgg ttgcgatcgg
     1321 tcacgagcgc tcgacctttg ccgtgtcgga tggacgggtc tgcgagttca cgcgtgttct
     1381 cgaatggggt ggttccgcgc tgaacgtggc gatcgcccgg gcaatcgacg ccgcgccgtc
     1441 cgaagtcgag ggcgtaaagc gggcgcttgc cttgacggac gagatggtgc ccgatggtct
     1501 cacctctgat caggcgaaga acgctcgcga ggccatgcgc agggcgattc agacgttcgc
     1561 ccgcgagctc gtatcctcgc tccagtacta ccagaaccag cccggctcgc tgggcatcgg
     1621 cgagatcgtg ctgacgggcg gcactgccca cctgcccggt atctccggcg agctagagcg
     1681 cctgatcggc gtacacgtcc gagtcggcga tccgctcgcg cggatgaagg tttcgaagaa
     1741 ggtcggccag ccggagcaga tcggctcgtt ggccgtcgcc atcggactcg ggatcgagga
     1801 ctagcgccgt gcgtgccgtc aacctcctgc cgcgagatca aaaccagcgc acgatcaaaa
     1861 aggagagcct cccggtcctg atcggcgcct gctccgccgt tctggtcgca gcggttctcg
     1921 gcgcgatgtt catgatgggc agcggtaagg tcgcagctca gcaacggaaa cttgacgatc
     1981 tgaaccgcca gtaccaggcg ctcccgccgc ctcccgcagg gccttccgtc gcacagcagc
     2041 agcttgccgg cgagcagagc gcccgcgtga gcgcactgag caccgcgctg acgagccgcg
     2101 tcgcctggga ccgggtcttt cgcgagttct cgctcgtgct tcccgacgat gtctggctga
     2161 caactctgac tgcgaagtcg ccgatctcgc cgctgacgaa cgtttcgacg agctcgcctg
     2221 gaggcgctcc ctcccagttc accatccagg gccgcaccta ttcgcacgac ggagtcgcgc
     2281 gactgctgtc acggctgcag gtcgtcccgg atctgacgaa cgtgcagctc cagagctcga
     2341 ccctcacaaa ggtcggcggt cagaacgtcg tcgacttctc gatcgcagcc gacatcaagg
     2401 tctccggagc cccgggcgca tgaagatcga catggacacg atcatgaaac gcatcaaggc
     2461 gctctccccc ggggcgttga tcgggatcat ggtcggcgca gtcctggtgt tcggcctggc
     2521 cgggtggttc ctcctcgtgc gcccgcaggg cgcgaaggtc acttccttga agactcaggc
     2581 gacggaggtc caggcgaaga tcgacgcgta ccaccagcag gtcgccgccg cgcgcgcggc
     2641 tccgaagatc gaggtggcgg acgtctaccg gctcgcgaag gcgatgccgg accggacaga
     2701 catgcccgac ctcctcctcg agctgagcca gctcgcgcgt gacaccggca ttcgcttcga
     2761 ttcgatctcg ccgcagacga tcgctccgat cgcgagctac acggtgctgc cgatctccgt
     2821 cacgttccag ggcaacttct acaacctggc ggacttcctc tatcgcctcc gctcgctcgt
     2881 gtccgtgcac gccggacgcc tcgacgcaac cggccgcctg ttctcggtcg acaccctgac
     2941 gttcaacgag agcacgctga agttccccca gatccaggcc accctcgtca tcgacgcgtt
     3001 cgtctatgga acaggcgtgc cggccacggc cccgattccg acgacgccga ccacgaccac
     3061 gacgacgacc gcggccacga ccacgaccac gaccgagacg cccccgagcg gcgcgtcggc
     3121 gacaggagct ccctgatggc gaagaaacac gtcgatccgc tcaaggcaaa gcagaagaag
     3181 cagaagatca tcgccgcggt gctcggcgtg gctttcctcg ggttgatggc ctttcaggta
     3241 ccccgagtca tgaagatgat gaagaccccg ccgccgccgg tccaacccac ggccgcgacg
     3301 gcgaccaccc ctgccggtgc tcccacactc gccgcaccga cgttgggcgg tgctcagcag
     3361 cctggcccgg cgacggactc gacgggctcg ctggccgcag cccccgcaac gaccgtgcag
     3421 gacggccaac tcgcgtcatt cagccgcttc gtgagcaagg atccgttctc gcagcagctc
     3481 agcgacacgc cgagcccctc ttcttcgacc gggtcatccg gcagctcctc aagcggagga
     3541 ggcgccgggc ccagggcgcc ggccgtccac ggcaacgccc cagcgccggg cagcgcagtc
     3601 atctcggtga acgggacgct ctacacggtt gcgacgaaga ccgacttccc gcagccgact
     3661 ccggtgttcc acctggtgtc ggtgaccgcc cacacggcga agatctcgat cgccggaggc
     3721 tcgtactcat ccggcgcgca gacggtcacg ctgcgggaga acaagcccgt gacactgatg
     3781 aacacggccg acggc
//
LOCUS       DASVEU010000084         7137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_256302,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000084 DASVEU010000000
VERSION     DASVEU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256302"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VF225_03245"
     CDS             complement(<1..393)
                     /locus_tag="VF225_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7144299.1"
                     /translation="MARTRVARNLLELVGNTPLVELQPVGDVQVYAKLEGQNPTGSIK
                     DRIAKAMIEAAEAAGELEPGRELLEPTSGNTGISLALVAKLKGYPLTCVMPENATEER
                     KRLLRLYGANIVFSPGDEGSNGAVRLALE"
     gene            complement(386..655)
                     /locus_tag="VF225_03250"
     CDS             complement(386..655)
                     /locus_tag="VF225_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX7144300.1"
                     /translation="MTKVRIPPTLREQAGGQRELAAEGDTVRELLEDLMGRFPALRTQ
                     LVEDGDIAPFVNVYLEGEDVRTLDGLDTEVRPGSTVILLPAMAGG"
     gene            complement(676..1824)
                     /gene="moeB"
                     /locus_tag="VF225_03255"
                     /pseudo
     CDS             complement(676..1824)
                     /gene="moeB"
                     /locus_tag="VF225_03255"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            1884..2669
                     /locus_tag="VF225_03260"
     CDS             1884..2669
                     /locus_tag="VF225_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144301.1"
                     /translation="MRVWIVVGLAATAAAGTVVGVTLATRSDVQRQSSKPPPFAADPT
                     ARPEVTQQVREALKAWPAGTARRLRILAARYPHSALVQLELGLALALSGQQADAARAW
                     QDAERVQPDSPSAVRAQDLRHPGSAPGLPPFVPSFTRVTNVVQAHLLRGSAYQQVLRP
                     VSAEREFLAAVRAAPNDPEALTAAAVGLYDKDRPAAAFSRLGPLARRFPQAQTVRFHL
                     GLLLIYFGDMPRARRELALARAQGPRTLLGKRAATLLKAGRKP"
     gene            2824..3849
                     /locus_tag="VF225_03265"
     CDS             2824..3849
                     /locus_tag="VF225_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144302.1"
                     /translation="METDDAKALLEAGKENGHLSADEIAVALDELDLEPAQIDDFYHA
                     LEELQIEVVAVEEEEQPVEAPREVSTDALQLFLKDIGKVDLLTAAQEVELAKRIERGD
                     HSAKQEMVEANLRLVVSIAKKYRNQGLPFLDLIQEGTIGLVRAAEKFDYRKGFKFSTY
                     ATWWIRQAVARALADKARTIRMPVHVVEKLNKIVRSERKLRAELGREPTSVEIGRDLD
                     LTGDEVEHIRRSAQTPVSLEKPVGDEDESEFGHFLTDANLPLPDEEAEITMRKETLRK
                     ILGTLSSRERQVLELRYGLDGQHPRTLDEVGRTFNVTRERIRQIENQSLKKLRALADA
                     VKLREVA"
     gene            4022..5347
                     /locus_tag="VF225_03270"
     CDS             4022..5347
                     /locus_tag="VF225_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7144303.1"
                     /translation="MEQTWVDELREFIAIPSVSADPAHQADVRRAAEWVRDFVKRSGG
                     EAELVPYGERDLVIGDIPANANGGNAPTVMIYGHFDVQPPAPLDLWETPPFELAERDG
                     WYYARGIADDKGQLFTLLKAAQLLREQNALPVNIRVVTDGEEEIGGHSVVDFLEADER
                     GADVCIIFDGGMTRPEQPEFGLATRGLVAFHLKVRTGERDMHSGYYGGAALNAIHVLM
                     QGLSAVTARDGLLPDPLRVGRTPLSQAEVDSLESLPKGWEMLDDAGATPLDAKAAEDF
                     YDRTWAEPSLDVNGIRGGKPEFLNTTLIVEAEARFTIRLAPGQDPETIAATVEKLIRA
                     AMPATAEVELIRENSAAPGVFSADSPAIQLGMEAFKRATGVRPLLVRVGGTLPIYPAL
                     AAKGIPTIGTGFALRESNVHSPNERLRVQDIDRAVAAASELYRGLAALG"
     gene            5340..6653
                     /gene="pepT"
                     /locus_tag="VF225_03275"
     CDS             5340..6653
                     /gene="pepT"
                     /locus_tag="VF225_03275"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX7144304.1"
                     /translation="MADPRYPTQIAAELADDVLSRFLRYVQVDTQSDDDSETYPSTAK
                     QLDLGKILADELREIGLEDVELTEHGYVFATVPGSAGPTVGLIAHMDTSSDESGTNVK
                     PQVIRNYDGGDIVLPGDPRKVLRPSENPILAERTGHDIVTTDGTTLLGADDKAGVAEI
                     MGAVAYLAAHPEIEHAPIRVGFTVDEEVGRGVDHFDIEEFGADFAYTLDGSEIGRIDD
                     ETFAASEVRIRIEGQSVHPGTSKGKMVSAIKLAARIVERLPQDERSPETTEGREGFVH
                     PTRIAGTTAEAELRFIARDFDAKKLEEHEQLLRKLADEVAAEEPRAHVTVTVEESYRN
                     MKEFLDADPAVTEAADEAARRAGMEPWREPIRGGTDGSMLSARGLLTPNIFTGGHQFH
                     SVLEWASVQDMAASTATIVELVKLWAEPEWLSRSRTHGRTAASPR"
     gene            complement(6607..6942)
                     /locus_tag="VF225_03280"
     CDS             complement(6607..6942)
                     /locus_tag="VF225_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144305.1"
                     /translation="MAMRTMNPVHESTSCAVAACAAIIGAKWTALLVHDLSEGPRRFS
                     ELEHSCAGISPRTLSERLRALEDEELVLRRSYAESPPRVEYELTERGAALLPIIEEMR
                     RFGHAFLTD"
BASE COUNT         1218 a         2361 c         2453 g         1105 t
ORIGIN      
        1 ctcgagcgcg aggcgcactg caccgttcga tccttcgtcg ccgggtgaaa agacgatgtt
       61 cgcgccgtag aggcgcagca gtcgcttgcg ttcctcggtc gcgttctccg gcatgacaca
      121 cgtgagggga tatcccttca gcttggcaac gagcgccagc gagatgccag tgttgccgct
      181 cgtcggttcg agcagctctc gtcccggctc caattccccg gctgcctcag ccgcctcgat
      241 catcgccttg gcgatgcggt ccttgatcga gccggtcggg ttctgccctt cgagcttcgc
      301 gtagacctgg acgtcgccga caggctgtag ctcgacgagc ggagtgttgc cgacgagctc
      361 gaggaggttg cgcgctacgc gcgtcctagc caccggccat ggccggtagc aggatcacgg
      421 tcgagccggg gcgcacctcg gtgtcgagcc cgtcgagcgt gcgcacgtcc tcgccctcga
      481 ggtaaacgtt cacgaacggt gcgatgtcac cgtcctcgac gagctgcgtg cggagcgccg
      541 ggaaccgtcc catcaggtcc tcgagcagct cgcgcacagt gtcgccctcg gcggcgagct
      601 cacgctggcc ccctgcctgc tcacgcagtg tcggtgggat ccggaccttt gtcatgccgg
      661 ctccctcgtg cctgcgcaga actcgacgta gtcgacgtac tcggtgatcg tcggatgctc
      721 gccgcagacc ggacactccg ggtcgcgccg tagcttcacc tcgtcgaacg tcgcggcgag
      781 tgcgtcgaag aggagaaggc ggccgatgag cggttcgccg atcccgagcg cgagcttgag
      841 cgcctcgctt gcctgcagcg aaccgatcac gcccggaagc acgccgagca caccgccctc
      901 tgcgcacgac ggcgcgagct ccggcggtgg cggcgtgggg aagaggcagc ggtaacaggg
      961 gccttcgtac ggcttgaaga ccgttacctg accctcgaag cggtagatcg atccgtggac
     1021 gaccggaatc ttgtgccaga cggacgcgtc gttgacgagg tagcgcgtcg ggaagttgtc
     1081 ggcgccgtcg acgatcacgt cccagccgtc ggccaggatc cgctcgatgt tctccgaggt
     1141 gaggcgctcc ttgtaagcga cgacctcgac gtcgggattg agcgcttcga tcgcgcgttt
     1201 cgccgactcg accttgggct cgccgagcga ctccgtcgag tggacgatct ggcgctgcag
     1261 gttcgactcg tccacgacgt ccgcgtcgac gatcccgatg cgaccgacac cggcggcagc
     1321 gagatagagg gacgcgggcg agccgaggcc gcccgcgccg atgagcaaga tcttcgagtc
     1381 gagcagcttc agctgcccct cctcgcccac ctccgggatc agcaggtgac ggctgtagcg
     1441 cgcgcgttgt ggagcgtcca ggccggtctg gagctgcacg gggaagccgt tgcgcttcca
     1501 gtcggtgaat ccgccggcga gcgagacgac gtcctcgtag ccgaggtcgg cgagcgtctt
     1561 ggccgcgaaa gccgagcggt tgccggccga gcagtagacg accactggcc ggctcgcatc
     1621 gggcgccagg cgctcgattc gggactccag atggccgcgc gggatgtgga cggccccggc
     1681 gatgtggccc tcgtcccact cgtcctgctc ccggacgtcg acgacgacgg gctcgcccga
     1741 gtcgatccgg tcccgggcct gggtcgcgtc gatctccgag atctcggacc gcgtctgctg
     1801 caggagttct cgatacgtcg gcatcgctat aggaaatata gctttgcgca cgtgagctag
     1861 cgtcgcctgc gatgagcgcg cgagtgagag tgtggattgt cgtcgggctc gccgccacgg
     1921 ccgctgccgg aaccgtggtc ggcgtgacgc tcgcaacgcg gagcgacgtc cagcggcagt
     1981 cgagcaaacc gccgcctttc gctgcggacc cgaccgcgcg gccggaggtg acccaacagg
     2041 tgcgcgaggc tctgaaggcc tggccggcgg gcaccgcccg tcggttgcgg atcctcgccg
     2101 cgcgctatcc ccacagcgcg ctcgtccagc tcgagctcgg gctagcgctc gcgctttccg
     2161 gtcagcaggc cgacgcggcg cgcgcctggc aagacgcgga gcgtgtccag cccgattccc
     2221 cttcggccgt ccgcgcccag gatctccgtc atccgggcag cgccccgggc ctgccgcctt
     2281 tcgtcccgag cttcacccgc gtcacgaacg tcgtccaggc ccatctgctg cgcggctccg
     2341 cgtaccagca ggtcttgcgc cccgtctccg cggaacgcga gtttctcgct gcggtccggg
     2401 ccgctccgaa cgaccccgag gccctgactg cggcggccgt cgggctctac gacaaggacc
     2461 ggccggcggc ggccttttcg cggctgggcc cgctcgcccg ccgctttccg caggcccaga
     2521 cggtgcgctt ccacctcggg ctcctgttga tctacttcgg agacatgcct cgggctcgtc
     2581 gtgagctagc cctcgcccgt gcgcaaggcc cacgaaccct cctcggaaag agagccgcta
     2641 ctcttctcaa ggcaggtcga aagccctagg accatcgggc acaaagatga gccgttcggc
     2701 ctatggcggt tgtccgaccc agctggcaca gtgcggcatc gctaaaacgc cggtcctgga
     2761 ggggggaacg gagtgagcgc aactgaggta gccgtagcat caccgaggac cgtcaccgat
     2821 cttttggaga cggatgacgc aaaagcgctt ctggaagcgg ggaaagagaa cgggcatctg
     2881 agtgccgacg agatcgccgt cgcgctcgac gagctcgatc tcgagcctgc tcagatcgac
     2941 gacttctacc acgctctgga ggagctccag atcgaggtgg tcgccgtcga ggaggaagag
     3001 cagccggttg aggcgcctcg cgaggtttcg accgatgcac tgcagctctt cctcaaggac
     3061 atcggcaagg tcgatctcct caccgccgcg caggaggtcg agctcgcgaa gcggatcgag
     3121 cgcggcgacc acagcgcgaa gcaggagatg gtcgaggcga accttcgcct cgtcgtctcg
     3181 atcgccaaga agtacagaaa ccagggtctg ccgttcctcg acctgatcca ggagggaacg
     3241 atcggcctcg ttcgcgcagc cgagaagttc gactaccgca aaggcttcaa gttctcgacg
     3301 tatgcgacgt ggtggatccg ccaggcggtc gcgcgtgcgc tcgccgacaa ggcgcgcacg
     3361 atccggatgc ccgtccacgt ggtcgagaag ctgaacaaga tcgttcgctc cgaacggaag
     3421 ctccgcgcgg agctcggccg cgagccaacc tcagtcgaga tcggcagaga cctcgacctc
     3481 acgggcgacg aggtggagca catccgccgc agcgcccaga ccccggtgtc gctcgagaaa
     3541 ccggtcggtg acgaggacga gtccgagttc gggcacttcc tcaccgacgc gaacctgccg
     3601 ctgccggacg aggaagccga gatcacgatg cgcaaggaga ccctgcgcaa gattctgggc
     3661 acgctctcga gccgggagcg ccaggtgctg gagctgcgtt acgggctcga cgggcagcac
     3721 ccgaggacgc tggatgaggt cgggcggacg ttcaacgtca cccgcgagcg catccggcaa
     3781 atcgagaatc agagcctgaa gaagctccgg gcactcgccg acgcggtcaa gctccgcgaa
     3841 gtggcatagc caaatagact tccccatatc aggaagaagg tcgcctgtgt cctggttgcg
     3901 gcgttcgcgg cagcgatcct agcctcccct gtgttcgcac agggcggcaa ggccggctcc
     3961 acgatcgtcg cgggcaggat ctagccagaa tcagagcagg gtccacgtag actcgccgcc
     4021 cgtggagcag acttgggtcg acgagctgcg cgagttcatc gcgattccga gcgtcagcgc
     4081 ggatccagct catcaagccg acgtgcgccg cgcggccgaa tgggtgcgtg acttcgtcaa
     4141 gcgttcgggt ggggaggccg agctcgtccc ctacggcgag cgggatctcg tcatcggcga
     4201 cattcccgcg aacgcgaacg gcggcaatgc gccgaccgtg atgatctacg gccacttcga
     4261 cgtccagccg cccgcgccgc tcgacctctg ggagacgccg ccgttcgagc tcgccgaacg
     4321 cgacggctgg tattacgcgc gcggcatcgc cgacgacaag ggccagctct tcacgctcct
     4381 gaaggccgcc cagctacttc gagagcagaa cgcgctgccg gtgaacatca gggtcgtcac
     4441 ggacggcgag gaggagatcg gcggtcactc ggtcgtcgac ttcctcgagg cggacgagcg
     4501 cggcgccgac gtctgcatca tcttcgacgg tgggatgacg cgcccggagc agcccgagtt
     4561 cgggctagcg acgcgcggcc tcgtcgcctt ccatctgaag gtacggaccg gcgagcgcga
     4621 catgcactcc ggctactacg gcggtgcggc gctcaacgcg atccacgtcc tgatgcaggg
     4681 actgtcggcg gtgactgcgc gtgacggact gctgcccgac ccactccgcg tcggcaggac
     4741 gccgctgagc caggccgagg tcgacagtct cgaaagcctt ccaaagggct gggagatgct
     4801 cgacgacgca ggcgcgacgc cgctcgacgc gaaggcggca gaggatttct acgaccgcac
     4861 gtgggccgag ccttcgctcg acgtcaacgg catccgcggg gggaagcccg agttcctcaa
     4921 cacgacgctg atcgtcgagg cagaggcgcg cttcacaatt cggctggcgc cgggccagga
     4981 tcccgagacg atcgccgcca cggtggagaa gctcatccgc gctgcgatgc cagcgacggc
     5041 ggaggtggag ctgatccgcg agaactcggc ggcgcccggc gtcttctctg cggactcgcc
     5101 cgcgatccag ctcgggatgg aagcgttcaa gagggcgacc ggggtgcgcc cgctgctcgt
     5161 gcgtgtcggc ggcacgctgc cgatctatcc ggcgctcgcg gcgaaaggca ttccgacgat
     5221 cgggacgggc ttcgcgctgc gcgagagcaa cgtccactcc cccaacgagc ggctgcgtgt
     5281 ccaggacatc gatcgtgcgg tggcggccgc atcggagctc taccgagggc tggccgccct
     5341 tggctgatcc gcggtatccg acgcagatcg ccgccgagct cgcagacgac gtgctcagcc
     5401 ggttcctgcg ctacgtccag gtcgacacgc agtcggacga cgactccgag acgtacccga
     5461 gcacggcgaa gcagctcgac ctgggcaaga tcctcgccga cgagctgcgt gagatcgggc
     5521 tcgaggacgt cgagctcaca gagcacgggt acgtcttcgc gacggtgccc gggtcggcgg
     5581 gcccgaccgt cgggttgatc gcccacatgg acacgagctc cgacgaatcc ggcaccaatg
     5641 tgaaaccgca ggtcatacgc aactacgacg gcggcgacat cgtcctgccc ggcgacccgc
     5701 gcaaggtcct gcgtccgagc gagaacccga tcctggcgga gcggacgggg cacgacatcg
     5761 tcaccaccga cgggacgacg ctgcttggtg cggacgacaa ggccggcgtc gcggagatca
     5821 tgggcgcagt cgcgtacctt gcggcgcatc cggagatcga gcacgctccg atccgcgtcg
     5881 gcttcacggt cgacgaggag gtgggacgcg gcgtcgacca cttcgacatc gaggagttcg
     5941 gggcggactt cgcgtacacg ctcgacggct cggaaatcgg tcgaatcgac gacgagacgt
     6001 tcgctgcctc ggaagtgcgc atccggatcg agggccagag tgtccatccg ggcacctcga
     6061 agggaaagat ggtcagcgcg atcaagctcg cagcgagaat cgtggagcgc ctccctcagg
     6121 acgagcgctc cccggagacg acggagggac gtgaaggctt cgttcatccg acacggatcg
     6181 cgggcacgac cgccgaggcc gagctgcgtt tcatcgcgcg cgacttcgac gccaagaagc
     6241 tcgaggagca cgagcagcta ttgcgcaagc tcgcagacga ggtcgcggca gaagagccgc
     6301 gcgcacacgt gacggtgacg gtcgaggaga gctatcgcaa catgaaggag ttcctcgatg
     6361 cggatcccgc ggtcaccgag gcggccgacg aggcggcgcg gcgcgcgggg atggagccct
     6421 ggcgcgagcc gattcgcggc ggcaccgacg gctcgatgtt gagcgcgcgg ggcttgttga
     6481 caccgaacat cttcacgggc ggccaccagt tccactccgt gctggagtgg gcgtcggttc
     6541 aggacatggc agcgtcgacc gcgacgatcg tcgagctcgt gaagctgtgg gcggagcccg
     6601 agtggctcag tcggtcaaga acgcatggcc gaaccgccgc atctcctcga tgatcggcag
     6661 cagcgcggcg cccctctcgg tcagctcgta ctccacacgc ggcggcgact cggcatagct
     6721 gcgacgtagg acgagctcct cgtcttcgag cgcccgcaga cgctcggaga gcgtgcgcgg
     6781 gctgatgccg gcgcaggagt gctcgagctc ggagaaccgg cggggccctt cggaaagatc
     6841 gtggacgagg agcgccgtcc acttcgcgcc gatgatcgcg gcgcacgctg cgacggcgca
     6901 ggacgtcgac tcgtgaacgg gattcatggt ccgcattgcc atggctttcc ttattgtagt
     6961 cagctaggcc cacggagggg ggttgtgcac ccagcgaccg ccgagcatcg tcgcaacgac
     7021 gttgacttcc ggtagttgtt cggcctcgag ctcgtatggg tcgcggtcga ggacgacgag
     7081 atctgcgagg aagccgggaa ggagcttccc gcggcgatgc tcgtcgcggg caagcca
//
LOCUS       DASVEU010000085        11399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_62205,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000085 DASVEU010000000
VERSION     DASVEU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62205"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VF225_03285"
     CDS             <1..722
                     /locus_tag="VF225_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7144306.1"
                     /translation="AWLYRPNVDGKVPVVLSIHGGPEAQERPVYQPLYQYLLSRGIGV
                     LATNIRGSTGYGKSYQRLVQRDWGGGDLQDWEHAVQWLRDQDWVEPERIGVYGGSYGG
                     FAVLTCVTRLPQYWAAAVDIFGPSNLVTFAKAVPPTWKRFIARFVGDPETEADFLMER
                     SPITYVDNVRTPLLVIQGATDPRVVKGESDQLVDKLKSLGRDVEYVVFDDEGHGFTKR
                     ANELKAYGLAAEWLERHLLAT"
     gene            complement(724..1491)
                     /locus_tag="VF225_03290"
     CDS             complement(724..1491)
                     /locus_tag="VF225_03290"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX7144307.1"
                     /translation="MEKVARFGLVAQGISFGLVAVLAIELAVGKGGKATDREGALRAL
                     AANGLGRIVVFILAFGFAAYAVWRLAQVFLGHELEEHGGRKKWGKRLSSFGKATIYGA
                     LSWGAFSIALGGRGGGHKEQQATKDILGWPGGRWIVSAIALAIAGAALWNFYRAVSGK
                     YKDSLKTGQMSARELKWTTRIAFVGLMSRAVIFGLISWFFFKAAAEYDAKKARGLDGA
                     LQKLAHAPYGTALLSLVAAGLFAYGVFCLVQARYREV"
     gene            complement(1529..2833)
                     /locus_tag="VF225_03295"
     CDS             complement(1529..2833)
                     /locus_tag="VF225_03295"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7144308.1"
                     /translation="MDVDADALIEQSRQLAVTPLGDRDRLASLLLGGSFLAVSLALAV
                     LAHSTRQIGFLTLAVFVASYALASRIDFEIGTGSAVPTQLVLVPMLALLPVQYVPLCV
                     AVGLLLGGLPDYARGRVPVDRSVLRLVNSWHAVGPALVLVAAGQPDPTPRNLPIYALA
                     LVAQFAFDFGSTALRDRLGLGVSPWSLLRFMVWIWAVDSALTPISVLAALGCGAHPSL
                     VVVSLPLIGLLAYFARERQVRIDHALELGHAYRGTAMLLGDVVEADDAYTGSHSRDVV
                     TLVLEVADKLGLDVHERRDAEFAALLHDIGKIKVPGEIINKPGKLTDEEWEIMKTHTV
                     EGERLLSQVGGILGNVGRIVRSCHEDWDGTGYPDGLAGEEIPLVARIVRACDAFSAMT
                     TDRSYRKARPADEAIAELRRCSGTDFDPAVVDALAATVSATA"
     gene            3063..3650
                     /locus_tag="VF225_03300"
     CDS             3063..3650
                     /locus_tag="VF225_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144309.1"
                     /translation="MRAVIVLGFVGGTIAMAMGAAPASAGLGLACPSATSTPFAGWKD
                     YANYAFAPDGGFESGGSGWALSGGARAVSGNESFYVHGNSDKTSLSLPAGASATSPGM
                     CISILSSKMRFLARGDAGSSVKVQILYRGLLSSVLGILDGGTIPAGGTWKPSPQIGML
                     GGVLPLLTASVSFRFTAVGGAAAIDDVYLDPMKSS"
     gene            complement(3713..4744)
                     /locus_tag="VF225_03305"
     CDS             complement(3713..4744)
                     /locus_tag="VF225_03305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7144310.1"
                     /translation="MSWVEAELREQPVALERFLTAESANTPGIVRALLRDDVRYLLIV
                     SRGSSSNVARYMQYLFGTVNRLPVSFATPSLYTVYDAPPELGPAAVIGISQSGASPDV
                     VAVLDEARKQNRPTLAITNDPKSPLALAADCVLQLHAGEERAVAATKTYLNSIAAIAL
                     LSTADDPKRLADLLGMPEKVGEQIEQSLAAAGALDDYRDVEGGSVVARGVNYGAAFEI
                     ALKIRELSGAQFEAFSSADLLHGPIAALKSGRPAIVIAPAGRTLASMSIALDKVRERG
                     AEVIAISDDARFLAESDTALPLVPGVPEWLTPLLTVVPGQVAAVRLATLRNANVDSPV
                     GLTKITLTH"
     gene            complement(4731..6572)
                     /locus_tag="VF225_03310"
     CDS             complement(4731..6572)
                     /locus_tag="VF225_03310"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7144311.1"
                     /translation="MKRAGVSYFGVRIPRHARRDLEDIAARGYTAVLHTFSENDLAYY
                     RGTMAEIVASSHELGLEVQMCPWGVGRTFGGEAESRWVTFHPEACQVLDDGRRVATGC
                     LNNPAYRAFCKDWADAALEAGTDYVFWDEPHWTVPEHVGVDDPQRWACVCQVCVELSG
                     LDLSRGLTPEVEQFREGSIVDFLGELTAHVASRGGKNTICLLPLTEGPHGVSDWDSVA
                     SLPHLSVLATDPYWKNFDEPADSFVGRFAQLLADTCARHDVDAQMWLPSFGLTRDDIP
                     DLEAAIAATRAAGVEDVWTWGYEACGHMTHLATPDSPLVWEAVTAALTGAERTATERQ
                     RVDLRDLDLRPTAELVRLINDEDRTVAGAVADAAESIACAIDSIVERLEAGGRLVYVG
                     TGSSGRAAAADAAECGPTFSTDRIVAVTTEEEAAEDDTNAGPRELAALDVGAMDVVLA
                     VSASGRTPYTLAALEQASRAGAHTIGLACVRDSELARRAVQAVEVEVGPEVITGSTRM
                     KAGTAQKLVLNTISTIAMVRIGKTFGNLMVDVQTSNEKLRARARRAVAVATAAPEDEV
                     ADAIAAADGDTKVAIVSLLAGVDAQTARSRLNAARGVIREAVADVVG"
     gene            complement(6569..7327)
                     /locus_tag="VF225_03315"
     CDS             complement(6569..7327)
                     /locus_tag="VF225_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7144312.1"
                     /translation="MSKQVLTRQRVLELIDQLQVGEAIPSERRLSGELGISRLTLRAA
                     LDDLVRDGYLERRHGSGTFVSEPKIAQQLTLTSFSEDMRRRGMTAGSKTIELRETHAG
                     AAVGRALNVSPDSRVVLIRRLRLADGEPMALETLHVPAELVPGLSRELLENASFYELL
                     EKEYGVVIESGMQSIEPTVTNEEESELLGVPLHSPAFLFERTSRTAAGETVEFVRSLY
                     RGDRYRLVAELSQRRDRPVAAAPLLTDAVAEPTP"
     gene            7502..8785
                     /locus_tag="VF225_03320"
     CDS             7502..8785
                     /locus_tag="VF225_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7144313.1"
                     /translation="MKARFLVAAVLAAMLAVAATTASGGTSKGQADKIVVWLMVDAQS
                     GWPEAVATANRNFKAQHPGVDVDVQYQSWGNHLQKLDAGLAGGDAPDVIELGNSEMTK
                     YMAAGAFKTLTQSAFPNNKTWLKGLKTSCQYNGKLYGVPYYAGARAVIYRKDQYKGAG
                     IKSTPKSLAQFIADGKKLMKKYGKDSGYSAVYFPGKYWYAAMSFVYDYGGQIAIRTGG
                     KWKGTLNSPQAIAALNQVNVIAKTLTKASRTTDEANPQQSLVFSKGKVGSFIGNGWEW
                     PYALDEKLGNPSLANVVGAYPMPSHTPGKFMPSFLGGSDLAVPVTTKNQSLAVDWIKA
                     FTSSAAESEIAKAGNIANTTTLLAYNASNPKLAPFARAAKYSWFVPTSPNWANVENAN
                     VLQNMLVKIFTGRASVKAAANSASSQITRILNTGA"
     gene            8812..9768
                     /locus_tag="VF225_03325"
     CDS             8812..9768
                     /locus_tag="VF225_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7144314.1"
                     /translation="MASVAESEFPLASRRRRRFTRQRLGRALAPYALLAPAATAIGVI
                     LGYPLYLLVKMSFEKYGLFELIRHKGRWIGTANYAHIFHDGEFWRVLARTLVFTVVTV
                     SLTMVLGTAMALLLLQVSRWLRLALTTGLVLVWSVPVIVAVDIWRWMVDYEFGVANWT
                     LTKLHIGDFIRHDWFANPWTGFGVITALIVWGAIPFITITVYAGLAQVPQELLEAAAI
                     DGARPWAVFREVTVPLLMPIFVILTSLSIIWDFQVFQQIWVMLDFRPTNDYYTMAIYA
                     FHQSFQISEYGLGAAIAVVMVLFMFGATLVYLRQMLRTGEVQ"
     gene            9765..10655
                     /locus_tag="VF225_03330"
     CDS             9765..10655
                     /locus_tag="VF225_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7144315.1"
                     /translation="MSSVTQIAPTPVAPVASRGRGRRRRTRQFGWNLLGLLVLAVMVF
                     PVYWMVATAFKPGQQILSYTPQWIPTHPTLSNFRDAIHQPFFWNAVKNSLIVVSAVVA
                     LSVVLAFLASLALAKFRFYGRKAFIVLILGVQMVPLAALIIPLYILMSRLGQIDKLTG
                     VIAMYLTFVLPFTVWTMRGFLLGIPKELEEAAMVDGATRFGAFVRVLLPLVGPGLVAT
                     SIFAFIQAWNEFIIAYVFLHTPEKQTLMVWLASFTTLRGTDWGPLMAGATLCALPVVV
                     FFLLVQRRIAFGLTAGAVRG"
     gene            10819..>11399
                     /locus_tag="VF225_03335"
     CDS             10819..>11399
                     /locus_tag="VF225_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7144316.1"
                     /translation="MAQIELVHLSKVYGNDVTAVRDLNLDVAEGEFMVLVGPSGCGKT
                     TALRMIAGLEEITSGDIRIGGKVVNDLAPRDRDIAMVFQNYALYPHKSVYENLAFGLR
                     MRKVPKDEQKRRVEEIARILGLGDMLQRRPAQLSGGQRQRVAMGRAIVREPKAFLMDE
                     PLSNLDAKLRVQMRAEIARIQQALKVTTVYVTH"
BASE COUNT         1939 a         3742 c         3770 g         1948 t
ORIGIN      
        1 ccgcctggct gtacaggccc aacgtcgacg gaaaggtccc ggtcgtcctg tcgatccacg
       61 gtggccccga ggcgcaggag cggccggtct accagccgct gtaccagtac ctgctctcgc
      121 gcggcatcgg ggtgctcgcg acgaacatcc gcggctcgac ggggtacggc aagtcgtacc
      181 agcggctcgt gcagcgtgac tggggcgggg gcgacctgca ggactgggag cacgcggtgc
      241 aatggcttcg cgaccaggac tgggtggagc cggagcggat cggcgtgtac ggcggttcgt
      301 atggcggctt cgccgtgctg acgtgcgtga cgcggctgcc gcagtactgg gcggcggcgg
      361 tggacatctt tggcccttcg aatctcgtca cgttcgccaa ggcggtgccg ccgacgtgga
      421 agcgtttcat cgcgcgcttc gtcggcgatc cggagacgga agcagatttc ctgatggagc
      481 gttcgccgat cacgtacgtt gacaacgtac gaacgccgct gctcgtcatt caaggcgcga
      541 cggatccgcg tgtcgtcaag ggcgagtcgg accagctggt ggacaagctg aagtcactcg
      601 ggcgcgacgt ggagtatgtc gtgttcgacg acgaaggcca cggcttcacg aagcgcgcga
      661 atgagctgaa ggcgtacggc cttgcggccg agtggctcga gcgtcacctt ctcgctactt
      721 gatctagacc tcgcggtacc tggcctggac caggcagaac acgccgtagg cgaagagtcc
      781 ggcggccacg aggctgagca atgccgtgcc gtagggcgcg tgcgcgagct tctggagcgc
      841 gccgtcgagg ccgcgtgcct tcttcgcgtc gtattcggcg gccgccttga agaagaacca
      901 ggagatcaga ccgaagatga cagcgcgtga catcaggccg acgaacgcga tccgggtcgt
      961 ccacttcagc tctctcgcgc tcatctgtcc ggtcttcagc gagtccttgt acttgccgga
     1021 gaccgcccga tagaagttcc agagggcagc gcccgcgatc gcaagcgcga ttgcgctgac
     1081 gatccagcgg cccccaggcc agccgaggat gtctttcgtc gcctgctgct ccttgtggcc
     1141 gccgccgcgc ccgccgagcg cgatcgagaa ggcaccccag ctgagcgcac cgtagatcgt
     1201 ggccttgccg aacgacgaga ggcgctttcc ccacttcttc ctaccgccat gctcttcgag
     1261 ttcgtggccg aggaagacct gcgccagcct ccagacggcg tacgcggcga agccaaaggc
     1321 aaggatgaag acgacgatgc gtccgagccc gttggccgcg agcgctctta gcgccccttc
     1381 tcgatccgtc gccttgccgc ccttgccgac ggcgagctcg atcgcgagca cggcgacgag
     1441 tccgaacgag attccctgcg caacgagtcc gaaccgggca accttctcca cgtccgcacg
     1501 ttctcgcgcg caggcgcctt cctgaaacct aagccgtggc ggaaaccgtg gcggcgagag
     1561 cgtcgacgac ggccggatcg aagtcggtgc cggagcagcg ccggagctcg gcgatcgcct
     1621 cgtcagccgg acgcgctttg cggtacgacc ggtcggtggt cattgcgctg aaggcgtcgc
     1681 aggctctcac gatccgtgcg accagtggga tttcttcgcc ggccagcccg tccggatagc
     1741 ccgtcccgtc ccagtcctca tggcaggaac gaacgatcct gccgacgttc ccgagaattc
     1801 cgccgacctg tgaaaggagt cgctcgcctt cgacggtgtg cgtcttcatg atctcccact
     1861 cctcgtcggt cagcttgccc ggcttgttga tgatctcgcc gggaaccttg atcttgccga
     1921 tgtcgtgcag gagagctgcg aactccgcat ctctgcgttc atggacatcg agtccaagtt
     1981 tgtcggcgac ctcgaggacg agcgtaacca cgtcccggct gtggctaccg gtgtaggcat
     2041 catcggcttc gacgacgtct ccgagcagca tggcggtgcc tcggtacgca tggccgagct
     2101 ccagggcgtg atcgattcga acctggcgct cgcgcgcgaa gtacgcgagc agaccgatca
     2161 agggcagcga gacgacgacc agcgaggggt gagcaccaca gccgagcgcg gcaaggacgg
     2221 agatgggcgt cagtgccgag tcgacggccc agatccagac catgaagcgc aggaggctcc
     2281 aaggtgagac accgagacca aggcggtcgc gcagtgctgt gctcccgaaa tcgaaggcaa
     2341 actgggcgac taaagccaac gcgtagatgg gcaggttcct cggcgtcggg tcgggctgac
     2401 cggccgcgac gaggacgagc gccggaccga cggcatgcca tgaattcacg aggcgaagga
     2461 cactgcggtc gaccgggaca cggccgcgcg cgtaatcggg gaggccgccg aggagcaggc
     2521 cgactgcgac gcacagcggc acgtactgga ccggaagcag agcgagcatc ggcacgagca
     2581 cgagttgcgt gggcacagcc gacccggtgc cgatctcgaa gtcgatccgg gaagcgagcg
     2641 cataggacgc cacgaagacg gcgagcgtca agaacccgat ttgccgggtg gagtgagcaa
     2701 gaaccgcgag cgcgagagaa acggccagga acgagccgcc gagcaggagc gaggcaagcc
     2761 gatcgcgatc gccgaggggc gtgacggcca gttgccgcga ctgctcgatc aaggcatcgg
     2821 cgtcgacatc caccccgcct cgaagggcgc cgctttcggg caaggaggat ccagtcgcga
     2881 ccgatgtcgc cgccatctcc gcgatatcgg cgcccgattg ggatttcttg acccgctggg
     2941 taaatcaccc caacggggga agccgcggct ccgggcctga agaaactgag caaagcaact
     3001 ggacagggca actgcggcta tctgagggag tgaattctgt ggcgaatcct tttctccgac
     3061 gcgtgagagc ggtcatcgtt ctcggtttcg tcggcggcac gattgcaatg gcaatgggcg
     3121 ccgcgccggc ctccgccggc ctcggccttg cctgtccctc tgcgacctcg acccccttcg
     3181 caggctggaa ggactacgcg aactacgcct tcgcaccgga cggaggcttc gaatcgggcg
     3241 ggagcgggtg ggccctgagc ggcggcgcgc gtgcggtttc cgggaacgag tcgttctacg
     3301 ttcacggcaa ctcggacaag acctcgctgt cgctgcctgc aggcgcgtcg gcgacgagcc
     3361 cggggatgtg catctcgatc ctctcgagca agatgcgttt cctggcccgg ggcgacgccg
     3421 gatcgagcgt caaggtgcag atcctctatc gaggcctgct gtcgagcgtg ctcggaatcc
     3481 tcgacggcgg cacgatcccg gcaggtggga cgtggaagcc ttcaccccag ataggaatgc
     3541 tgggcggggt tcttccactg ctgaccgctt cggtctcgtt ccgcttcacg gcggtcggcg
     3601 gcgccgccgc gatcgacgac gtgtacctcg atccgatgaa gtcctcctag cgatcgaccg
     3661 cgcatgaact gtttcgctcg atggagctcg tgagaacgag ctccatcgcg tttcagtgag
     3721 tcagcgtgat cttcgtcagc ccgacaggac tgtcgacatt cgcgttgcgc agagtcgcga
     3781 gtcgcactgc cgcgacctgt cccggcacga ccgtcagtag cggcgtcagc cactcgggca
     3841 cgcccggaac caggggcagc gcggtatcgg actcggcaag gaacctcgcg tcgtcgctga
     3901 tcgcgattac ctccgcgcct ctctcgcgca ccttgtcgag cgcaatgctc atgctcgcga
     3961 gggtgcggcc ggcaggggcg atcacgattg cgggccggcc ggacttcagc gctgcgatcg
     4021 gtccgtgcag caagtctgcc gaggagaacg cctcgaactg cgcgcccgag agctcgcgga
     4081 tcttcagtgc gatctcgaac gccgccccat agttgacgcc gcgcgcgacg acgctcccgc
     4141 cctccacgtc gcgatagtcg tcgagtgcgc cggccgcagc cagcgactgc tcgatctgct
     4201 cgccgacctt ctcaggcatc ccgagtaggt ctgcgagccg cttcgggtcg tccgccgtcg
     4261 acagcaatgc gatcgcggcg atcgagttga ggtacgtctt cgtcgccgcc accgcccgct
     4321 cctcgcccgc gtgcagctgg aggacgcagt cggcggcaag cgccagcggc gacttcgggt
     4381 cgtttgtgat cgcgagcgtt ggacgattct gctttcgcgc ctcgtcgagg acggcgacga
     4441 catcgggtga tgcgcccgac tgcgagattc cgatcactgc ggcaggcccg agctccggcg
     4501 gtgcgtcgta gacggtgtac agcgacggtg tcgcgaatga gaccggcagg cggttgacag
     4561 tgccgaagag gtactgcatg taccgcgcga cgttgctcga gctgccgcgc gacacgatca
     4621 ggagataacg gacgtcgtct cgcaaaaggg cgcggacgat tccgggcgtg ttcgccgact
     4681 cggccgtcag gaagcgctcg agagcgacgg gctgctctct aagctcggcc tcaacccacg
     4741 acatctgcga ccgcctcgcg gatgacgcca cgcgccgcgt tgagccgtga ccgtgccgtc
     4801 tgcgcgtcga cgccggcgag cagcgagacg atcgctacct tcgtgtcgcc gtccgcggct
     4861 gcgattgcat ccgcgacttc gtcctccggg gcggcggtcg caacggcgac cgcgcgtcgc
     4921 gctcgcgcac gcagcttttc gttcgaggtc tgcacgtcga ccatcagatt tccgaacgtc
     4981 ttgccgatcc gcaccatcgc gatcgtggag atcgtgttca ggacgagctt ctgcgccgtc
     5041 cccgccttca tccgcgtcga gccggtgatg acctccggcc ccacctcgac ctcgactgcc
     5101 tggacggctc gccgtgcgag ctcggagtcg cggacgcagg cgagcccgat cgtgtgcgcg
     5161 ccggccctcg acgcttgctc gagggcggcg agcgtgtagg gcgtgcgtcc actcgcgctg
     5221 actgcgagca cgacgtccat cgcaccaacg tccagagccg cgagctcgcg cggacccgcg
     5281 ttggtgtcgt cctcggcagc ctcttcctcc gtcgtcacag cgacgatccg gtcggtcgag
     5341 aacgtcggcc cgcactccgc ggcatcggcc gccgcggcac ggcccgacga gccggtgccg
     5401 acgtagacga gcctcccgcc ggcctcgagc cgctcgacga tcgagtcgat cgcgcatgcg
     5461 atcgactctg cggcgtcggc cacggcgccc gcgaccgttc ggtcctcgtc gttgatgagc
     5521 cggacgagct cggccgttgg tcgcaggtcg agatcgcgga ggtcgacacg ctgtctctcg
     5581 gttgccgtac gttctgctcc agtcagtgcc gcggtgacgg cttcccagac gagcggcgag
     5641 tcgggcgtcg ccaggtgcgt catgtggccg caggcctcgt agccccacgt ccagacgtct
     5701 tcgactccgg cagcgcgggt cgcggcgatc gccgcctcca gatcggggat atcgtcgcgc
     5761 gtcagcccga agctgggcag ccacatctgc gcgtcgacgt cgtggcgagc gcaggtgtcc
     5821 gcaagcagtt gcgcgaagcg gccgacaaag gagtcggccg gctcgtcgaa gttcttccag
     5881 tacgggtccg ttgccagtac cgagagatgc gggagtgagg cgaccgagtc ccagtcgctg
     5941 acgccgtgcg gcccctccgt cagcggcagt aggcagatcg tgttcttccc gccgcgtgag
     6001 gcaacgtgcg ccgtcagctc gccgaggaaa tcgacgatcg atccttcacg aaactgttcc
     6061 acttccgggg tcagaccccg cgacaggtcc agtcccgaca gctcgacaca gacttggcac
     6121 acacaggccc accgctgcgg atcgtcgacg ccgacgtgct cgggcaccgt ccagtggggc
     6181 tcgtcccaga agacgtagtc cgtgccggcc tcgagcgcgg cgtccgccca gtccttgcag
     6241 aacgctcggt acgcaggatt gttgaggcaa ccggtcgcga cacgacgccc gtcgtccagc
     6301 acctgacatg cctccggatg gaaggtcacc cagcggctct cggcctcacc tccgaacgtc
     6361 ctcccgacgc cccacgggca catctgcacc tcgagcccga gctcgtgcga cgacgcgacg
     6421 atctcggcca tcgtgccgcg gtagtaggcg aggtcgtttt cggagaacgt gtgcaacacg
     6481 gcggtgtagc cgcgcgcggc gatgtcctcg agatcgcggc gggcgtgacg ggggatgcgg
     6541 acgccgaaat acgagacgcc cgcgcgcttc atggagtcgg ttccgcgacc gcgtcggtca
     6601 acagaggtgc ggcggcaacc gggcggtcgc gccgctgcga cagctcggcg acgagccggt
     6661 agcggtcgcc tcggtagagc gagcggacga attcgaccgt ctctccggcg gccgtgcggg
     6721 aggtccgctc gaagaggaag gccggcgagt gcaacggcac gccgagcaac tcggactcct
     6781 cctcgttcgt cacggtgggc tcgatcgact gcatcccgct ctcgatcacg acgccgtact
     6841 ccttctcgag cagctcgtag aaggatgcat tctcgagcaa ttcgcgggag aggccgggca
     6901 cgagctcggc gggcacgtgc agcgtctcga gcgccatcgg ctcgccgtcg gcaaggcgca
     6961 ggcgcctgat gaggacgacg cgtgagtcgg gggacacatt gagcgcgcgg ccgaccgcgg
     7021 caccggcgtg cgtctcgcgc agctcgatcg tcttgctgcc ggcggtcatg ccgcgacggc
     7081 gcatgtcctc gctgaaggag gtcagggtca gctgctgcgc gatcttcggc tcgctgacga
     7141 aggtgccgct tccgtgccgc cgctcgaggt acccgtcgcg gacgagatcg tcgagtgccg
     7201 cccggagcgt caggcgcgag atgccgagct cgccgctgag ccgccgctcc gaggggattg
     7261 cctctccgac ctggagctgg tcgatcagct cgaggactcg ctgcctggtc aagacctgtt
     7321 tgctcatggc gctctctatg gaagcacagc cgtccgcacg tggtcaagac cactcgcccg
     7381 cagtcagcgg agaattcacc cccttgacac caagattggt ctgtgccaat ctctcggccg
     7441 tggttggtgg tcttaaccaa tcggggaagc cagggtggct ccccatgctc cgggaggcga
     7501 catgaaagct cgattcctcg tggcggcggt cctcgctgcc atgcttgccg ttgcggcgac
     7561 gaccgcaagc ggcgggacgt caaaagggca ggcggacaag atcgtcgtct ggctcatggt
     7621 cgatgcacag agcggttggc ctgaggccgt tgccaccgcc aacaggaact tcaaggcaca
     7681 gcatccgggc gtcgacgtcg acgtccagta ccagtcgtgg ggcaaccatc tccagaagct
     7741 cgacgccggg ctcgcaggcg gtgacgcgcc cgacgtgatc gagttgggca actcggaaat
     7801 gacgaagtac atggccgccg gtgccttcaa gacactgacg cagtcggctt tccccaacaa
     7861 caagacctgg ctcaagggcc tcaagacctc gtgccagtac aacgggaagc tctacggcgt
     7921 cccctattac gccggcgcgc gcgcggtcat ctaccgcaag gaccagtaca agggcgcggg
     7981 catcaagtcg acaccgaaga gcctcgcgca gttcatcgcg gatgggaaga agctgatgaa
     8041 gaaatacggg aaggactcgg gctactcagc cgtttacttc cccggcaagt actggtacgc
     8101 agcgatgtcc ttcgtctacg actacggcgg acagatcgca atccgcacgg gcgggaagtg
     8161 gaagggaacg ctcaactccc cgcaggcgat agcggccctg aatcaggtca acgtgatcgc
     8221 caagacgctg accaaggcga gcaggacgac cgatgaggcg aacccgcagc agtcgctcgt
     8281 gttctcgaag ggcaaggtcg gctcgtttat cggcaacggc tgggagtggc cctacgcgct
     8341 cgatgagaag cttggtaacc ccagcctcgc aaacgtggtc ggggcctatc cgatgccgag
     8401 ccatactccg ggcaagttca tgcccagttt cctcggcggc tccgacctcg cggttcccgt
     8461 gaccacgaag aaccagagcc tggccgtcga ctggatcaag gcgttcacga gcagcgccgc
     8521 ggagagcgag atcgcgaaag cgggcaacat cgccaacacg acgacactcc tcgcctacaa
     8581 cgcctcgaac ccgaagctgg cgccgttcgc acgagccgcg aagtacagct ggttcgtccc
     8641 cacgagcccc aactgggcga acgtggagaa tgcaaacgtg ctgcaaaaca tgctcgtcaa
     8701 gatcttcacc ggccgcgcat cggtgaaggc agccgcaaac agtgccagta gtcagatcac
     8761 gaggatcctg aacaccggcg cgtgagtcgc ctcgcgcccg aaggagcagc tatggcgtcc
     8821 gtcgccgaga gcgagtttcc actcgcttct cggcggcggc gccgcttcac acggcagcga
     8881 ctcggacgtg cactcgctcc ctacgccctg ctggctccgg cggctacggc catcggcgtc
     8941 atcctcggct acccgctgta tctgctcgtc aagatgtcgt tcgagaagta cggcctcttc
     9001 gagttgatcc ggcacaaggg ccgctggatc gggaccgcga actacgccca catctttcac
     9061 gacggcgagt tctggcgtgt gctggcgagg actctcgtgt tcaccgtcgt cacggtgtcg
     9121 ttgaccatgg tgctcggcac agcgatggcg ttgctgctcc ttcaggtctc gcgctggctg
     9181 cgtcttgcgc tcacgaccgg cctcgtcctc gtctggtccg tgccggtgat cgtcgcggtg
     9241 gacatctggc gctggatggt ggactacgag ttcggcgtcg ccaattggac cctgaccaag
     9301 ctccacattg gcgacttcat caggcacgac tggttcgcga atccctggac cggcttcggc
     9361 gtgatcactg cgctgatcgt ctggggcgcg attccgttca tcacgatcac ggtgtatgcc
     9421 ggcctcgctc aggtgccgca ggagctgctc gaggcggcgg cgatcgacgg cgcccggccc
     9481 tgggccgtct tccgtgaggt gacggtcccc ttgctgatgc cgatcttcgt catcctcacg
     9541 agcctgtcga tcatctggga cttccaggtc ttccagcaga tctgggtgat gctcgacttc
     9601 cggccgacga acgactacta cacgatggcg atctacgcct tccaccagtc gttccagatc
     9661 agtgagtacg gcctcggtgc ggcgatcgcg gtcgtgatgg tgctgttcat gttcggagcc
     9721 acactcgtgt atctccgcca gatgctgagg acgggggagg tgcagtgagc tcggtcacgc
     9781 agatcgcgcc gacgccagtc gctcccgtgg cgtccagagg acgaggccgg cgacggcgaa
     9841 cacgccagtt cggctggaac ctgctcggcc tgctcgtgct cgccgtgatg gtctttccgg
     9901 tgtactggat ggtcgcgacg gcgttcaagc cgggacagca gatcctcagc tacacgccac
     9961 agtggattcc gactcacccg acgctttcga acttccgcga cgccatccat cagcccttct
    10021 tttggaacgc ggtgaagaac agtctcatcg tcgtcagcgc cgtggttgcg ctctcggttg
    10081 tcctggcttt tctggcatcg ctcgcgctcg cgaagttccg cttctacgga cgcaaggcgt
    10141 tcatcgtcct gattctcggc gtccagatgg tgccgctcgc ggctttgatc atcccgctct
    10201 acatcctcat gtctcggctg ggacagatcg acaagctcac cggggtgatc gccatgtacc
    10261 tcacgttcgt cctgcccttc acggtgtgga cgatgcgtgg cttcctgctc ggaattccga
    10321 aggagctcga ggaggcggcg atggtggacg gagcaacccg cttcggagcc ttcgtgcggg
    10381 tcctcctgcc tctcgtgggg ccggggttgg tagcgacgtc gatcttcgcc ttcatccagg
    10441 cctggaacga gttcatcatc gcgtatgtgt tcctgcacac gcccgagaag cagacgttga
    10501 tggtgtggct cgcgtccttc acgacgctgc gcggaacgga ctggggaccg ctgatggccg
    10561 gcgctacgtt gtgcgcactg ccggtcgtcg tcttcttcct gctcgtgcag cggcggatcg
    10621 cgttcggtct gaccgcgggg gcggtgcgcg gttagtgccc ctcccagcaa cccacgccgg
    10681 cctctggccc gcgaccacgc ggcgccagag tccaccctcg cccttagcaa ggcagaccag
    10741 ccttgctgcg gttgagtgcg gcctctggct tggtgctcgc gacccagaga ctcggcaggg
    10801 tcaccgggag gggcactgat ggcccaaatc gagctcgtcc acctctcgaa ggtctacggc
    10861 aacgacgtca ctgccgtgcg cgacctgaac ctcgacgttg ccgagggcga gttcatggtg
    10921 ctggtcggcc cgtcgggatg cggaaagacc acggcgctcc ggatgatcgc cgggctcgag
    10981 gagatcacgt cgggcgacat ccggatcggc ggcaaggtcg tcaacgacct cgcaccccgc
    11041 gaccgcgaca ttgcgatggt cttccagaac tacgcgctct acccgcacaa gagcgtctac
    11101 gagaacctcg ccttcggatt gcgcatgcgc aaggtaccga aggacgaaca gaagcggcgc
    11161 gttgaggaga tcgcacgcat cctcgggcta ggcgacatgc tgcagcgccg ccccgcgcag
    11221 ctgtcgggcg ggcagcgcca acgcgtcgca atgggacgcg cgatcgtgcg tgagccgaaa
    11281 gcgttcctga tggacgagcc actctcgaac ctcgacgcga agctgcgcgt tcagatgcgt
    11341 gccgagatag cccgcatcca gcaggcgctc aaggtgacga ccgtctatgt cacgcacga
//
LOCUS       DASVEU010000086         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_838825,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000086 DASVEU010000000
VERSION     DASVEU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838825"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VF225_03340"
     CDS             <1..359
                     /locus_tag="VF225_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144317.1"
                     /translation="ADRIQARIAHHVPNLESSILRRVVLSPADLQAANINLEHGDPYS
                     GSLALDQNFLWRPFARQPGHRTPIDRLFQIGASTWPGPGLGAGSGTLVAKALLQPPAV
                     QRLVGRLGAFVRPGQA"
     gene            430..1248
                     /locus_tag="VF225_03345"
     CDS             430..1248
                     /locus_tag="VF225_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7144318.1"
                     /translation="MRAYAAAGVDGIGIWEIKLPAGRDVEALAQLEASGLGSAAAIPA
                     VPSILPLPLMEGPEDPQERIDAIHASLHRLAPFEPSSVVCLTGPGEDRDLVVEGLQTI
                     GEEAARLGLRIGLEPVNRIGGENWTMISSLFEAVELLEAADRPALGIQFDSWHVWNTR
                     GLVEDIETYAHHFVGVHIADWSEPTRGWCDRVLPGDGVADLPRILGALDRAGWEGYYD
                     LEIFSDNGTFGNKWPDSLWDVPADEFARRGKDAFERVWEARIQTSLDQVSPGAV"
     gene            1285..2484
                     /locus_tag="VF225_03350"
     CDS             1285..2484
                     /locus_tag="VF225_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7144319.1"
                     /translation="MRVRGRWAFVTAALLAFVLIGSALAATAGHRTAQAKAPIIIGWA
                     FDGKGAMAPFDGPALAAAQLRVAQVNAKGGVNGRKLQIRTCDTQENAPATAKACALRL
                     LGQKANVLFTTCDVDFAAPVVRAAIDRGVLAIAPCIGTDQMGPKRFGPKGRLAFSFGN
                     VAQDEGSAMAQYAYSRGWRSAALATDTVIVYFKNVVQAFEVRFKQLGGKIVAKETYQS
                     LGGNNVQNAVSRLNDVKADVIVTSTAGAFGALSTLISGLRSLGNNTPILNSWAGDGTY
                     WLPANPKVTNYWFVTFASCFGDDPSAAVNTLAKQVKAGTGGFITGPSAIDGLVTAIKR
                     ANGSTKGAVLSKVMEHFKKVPTISGNVSFSAKYHTVFGRRYRVIRIQDNVPKVVGTIV
                     AKVVAKI"
     gene            2554..3264
                     /locus_tag="VF225_03355"
     CDS             2554..3264
                     /locus_tag="VF225_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144320.1"
                     /translation="MSRSFEGVHALRDVTLELRRREVVGLIGPNGAGKSTLVNLLSGF
                     DRPTAGMVELEGRDVTRWSPSRRGRHGLARTFQHSHAFRSLSVQENVEVAALGVGTAA
                     REARRRAMELLETLGLTSQADARADTLAQGDERRLGVARALATNPRFVLLDEPAAGLP
                     EAEVPELADAVRSVRDRHDAGVLLIDHNMTLIMAICDRIYVLDQGRALAEGTPEEVRG
                     NLDVAAAYLGESAVQEET"
     gene            3452..4165
                     /locus_tag="VF225_03360"
     CDS             3452..4165
                     /locus_tag="VF225_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144321.1"
                     /translation="MSPPALAVADLYVRHGAVDAVRGLSIEVGHGEIVGLIGPNGAGK
                     SSTLHAIMGAVRPADGDVRVNGASVVGRRPEDVARLGVALVPEGRRIFAELTVEENLL
                     LGLAGRRRREGVAEDTRRVYDLFPVVEEARRRKAGTLSGGQQQQLAIARALVADPEVL
                     LLDEPSLGLAPRVVDLVFDALAAIRERGLAILLVEQRAQRTVAFADRSHVLSKGQLRL
                     TLGPESAGETDALVSAYLA"
BASE COUNT          704 a         1391 c         1443 g          691 t
ORIGIN      
        1 acgccgaccg gatccaggcg cgcattgctc atcacgttcc gaacctcgag tcctccatcc
       61 tgcgccgcgt cgtgctctca cctgccgatc tccaggctgc gaacatcaac ctcgagcacg
      121 gcgaccccta ctcgggctcg cttgcgctcg accagaactt cctctggcgt ccgttcgccc
      181 gacagccggg gcacaggact ccgatcgacc gcctgttcca gatcggcgcc agcacgtggc
      241 ccggcccggg cctcggcgcc ggctcgggca ccctggttgc gaaggccctc ctgcagcctc
      301 cggcggtgca gcggctagtc gggcggctcg gcgcgttcgt gcggcccgga caggcataga
      361 atcgcctgca tccggccgcg tttctcgatc tcgcagatta cgaccctggc atcgacgttc
      421 gcggcggatg tgcgcgcgta cgccgcagca ggcgtggacg ggatcggcat ctgggagatc
      481 aagcttccgg ccggacgtga cgtggaggcg ctcgcgcagc tcgaggcgag cggcctcggc
      541 tcggccgcgg cgattcccgc agtgccgtcg atcctgccgc tgccactgat ggaaggtccc
      601 gaagatccgc aagagcgaat cgacgcgatc catgcctcgc tgcaccgtct cgccccattc
      661 gagcccagca gcgtcgtttg cctgaccggg ccgggtgagg atcgcgacct ggtcgtcgag
      721 ggtctgcaga cgataggcga ggaagccgca cgactcggtt tgcgcatcgg tctcgaaccg
      781 gtcaaccgca taggcggtga gaattggacg atgatctcgt cccttttcga ggcggtcgag
      841 ctgctcgaag ccgcggacag gcccgcgctc ggcatccagt tcgacagctg gcacgtctgg
      901 aacacccgcg ggctcgtcga agacatcgag acgtatgccc accatttcgt cggcgtccac
      961 atcgcagact ggagcgagcc cactcgcggg tggtgcgatc gcgtgctacc cggcgacggc
     1021 gtcgccgacc ttcctcggat cctcggcgcg ctcgaccgcg caggctggga gggctactac
     1081 gacctcgaga tcttctccga caacggcacg tttggcaaca agtggcccga ttcgttatgg
     1141 gacgttccgg ccgacgagtt cgcccggagg gggaaggacg cgttcgagcg cgtgtgggag
     1201 gcgcgaatcc aaacgtccct tgaccaggta tcgcccggtg ccgtgtaatc gacgaatgag
     1261 gacgacccca agaggaggga ctacatgaga gttcgtggga ggtgggcgtt cgtcactgca
     1321 gcgttgctgg cgttcgtcct gatcggaagc gcgctcgcgg cgacggcggg gcaccgtacc
     1381 gcgcaggcga aggcgccgat catcatcggt tgggcattcg acggcaaggg tgcgatggcg
     1441 ccgttcgacg gccctgcact ggccgcggca cagcttcgcg tcgcccaggt gaacgcgaag
     1501 ggcggcgtca acggacggaa gctccagatc cggacatgtg acacgcagga gaacgcgccc
     1561 gcaacggcca aggcgtgtgc gctgcgccta ctcggccaga aggccaacgt cctcttcacg
     1621 acctgtgacg ttgacttcgc cgcgccggtg gtgagagcgg cgatcgaccg cggtgtcctg
     1681 gcgatcgcac cgtgcatcgg caccgaccag atgggcccga agcggttcgg cccgaagggt
     1741 cgcctggcgt tcagcttcgg taacgtcgca caggacgaag gctccgcgat ggctcagtac
     1801 gcgtatagcc gaggctggag gtcagccgcg ctcgcgacag acaccgtcat cgtgtacttc
     1861 aagaacgttg ttcaggcctt cgaggtgcgg ttcaagcagc tcggcggcaa gatcgtcgcg
     1921 aaggagacgt accagtcgct cggcggcaac aacgtccaga acgcagtcag ccgtctcaac
     1981 gacgtcaagg cggacgtgat cgtcacttcg accgcaggtg cgttcggggc attgtcgacc
     2041 ttgatctccg gattgcggtc gctcgggaac aacacgccga tcctcaactc ctgggccggc
     2101 gacggcacgt actggctgcc ggccaacccg aaggtgacga actactggtt cgtcacgttc
     2161 gcttcctgct tcggcgacga cccgagcgcg gcggtcaaca cgctcgcaaa acaggtgaag
     2221 gccggaacgg gtggcttcat cacgggcccg tccgcaatcg acggtctcgt caccgcgatc
     2281 aagcgcgcga acggctcgac aaagggagct gtactgtcga aggtgatgga gcacttcaag
     2341 aaggtgccca cgatctcggg caacgtcagc ttctcggcga agtaccacac ggttttcggt
     2401 cgccggtacc gggtgatccg gatccaggac aacgtgccga aggtcgtcgg cacgatcgtg
     2461 gccaaggtcg tagcgaagat ctaggccaac ttgtcgcaaa cgagcggcgg cgaagcgacg
     2521 aggaggcccg gggacacact ccgggcctcc tccttgtcgc ggtccttcga gggcgtgcat
     2581 gcgttgcgcg acgtcacgct cgagctgagg aggcgggagg tcgtagggct gatcgggccg
     2641 aacggggcgg gcaagtcgac gctcgtgaac ctgttgagcg gcttcgaccg cccgaccgcg
     2701 gggatggtcg aactggaggg ccgcgacgtg acgcgctgga gcccgagccg gcggggacgg
     2761 cacgggctgg ccagaacctt ccagcacagc catgccttcc gctcgctgtc ggtgcaggag
     2821 aacgtcgaag ttgcggcact tggtgtaggc acggccgccc gcgaggcacg aaggcgcgct
     2881 atggagttgc tcgagacgct cggtctcacc tcgcaggcgg acgcgcgcgc cgacacgctc
     2941 gcacagggcg acgaacggcg actgggcgtc gcgcgggccc tcgcgacgaa tccgcgtttc
     3001 gtcctgctcg acgagccggc ggccggcctg ccggaggctg aagttcccga gcttgccgac
     3061 gccgtccgct cggtccggga tcggcacgac gccggcgtgc ttctgatcga tcacaacatg
     3121 acgctgatca tggcgatctg cgaccgcatc tacgtgctcg accaagggcg cgcgctcgcc
     3181 gaagggacgc cggaggaggt tcgcggcaat ctcgacgtcg ccgcggccta cctcggcgag
     3241 agcgccgtgc aggaggagac gtgaccgtcg ctcgccttcg gctcgctccc gtgggggaaa
     3301 ccatgttccc cccacgagcc cccttcttta gaagtcgcct caggggaagt ggcattgcga
     3361 ccgccgccac cacccccgag gagaaacaag ggggaaacct cccggtttcc ccttacgtcc
     3421 cttccaccgc tcataggcca gaggtcggcc tatgagccct cctgccctcg ccgtagcgga
     3481 tctctatgtg cgtcacgggg cggtggatgc ggtccgcggc ctgtccatcg aggtcgggca
     3541 cggcgagatc gtcggtctca ttgggccgaa cggggcgggg aagtcttcga cactgcacgc
     3601 gatcatgggc gcggtccgtc ccgccgacgg agacgtgcgt gtcaacgggg cctcggtggt
     3661 cggccggcga cccgaagacg tggcacggct cggtgtcgcg ctcgtgccgg aagggaggcg
     3721 catcttcgcc gagctgacgg tcgaggagaa tcttttgctc gggctcgccg ggcgacgcag
     3781 gcgggagggc gtggcagagg acacgagacg cgtctacgac ctttttcccg tcgtcgagga
     3841 agcgcgtcgc agaaaggcgg gaaccctctc cggcggacag cagcagcagc ttgcgatcgc
     3901 tcgggcactg gtcgcggatc cggaggtgct gctcctcgac gaaccttcgc tcggtctcgc
     3961 gccccgcgtg gtcgacctgg tcttcgacgc gctcgccgcg atccgcgagc gcggcctcgc
     4021 gatcctgctc gtcgagcagc gggcgcaacg gacggttgcg ttcgcggacc ggtcccacgt
     4081 cttgtccaaa gggcagttgc gcttaactct cggacccgag tccgcgggtg aaacggacgc
     4141 gctcgtcagc gcgtatctcg catgaacgtc gctcgccttc ggctcgctcc cgtgagggaa
     4201 accatgtttc cctcacgagc acccttctt
//
LOCUS       DASVEU010000087         5546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1227713,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000087 DASVEU010000000
VERSION     DASVEU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227713"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..466)
                     /gene="trxA"
                     /locus_tag="VF225_03365"
     CDS             complement(143..466)
                     /gene="trxA"
                     /locus_tag="VF225_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7144322.1"
                     /translation="MTTAVTEQSFEEEVLQSEKPVIVDFWAEWCGPCHAVAPVLDRIV
                     DERNGDLKLVKVNIDEQPALSQRYGVQSIPTMILFKDGEPAAAAIGAQPKGAIEKSLG
                     LVDES"
     gene            553..3597
                     /gene="ileS"
                     /locus_tag="VF225_03370"
     CDS             553..3597
                     /gene="ileS"
                     /locus_tag="VF225_03370"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7144323.1"
                     /translation="MFEPLPQYPDHPALEEEILAWWEEARIFDRLRERNRGGPHWSFF
                     DGPKTANTTDGLGVHHLWGRTYKDVFQRYKALQGFDQRYQNGFDCQGLWVEVKVERDL
                     GLNSKPEIEQYGMAAFARRCRELVAESARVITEQSIRLGQWMDWGNDYYTFSDTNIEY
                     IWRFLKTLDERGWLYQGHRSTEWCPRCGTSISQHELSQSEVYRDKSDPSLFVRFPLKG
                     RDRESLVVWTTTPWTLPANVAAAVNPDAEYGKQDTGEWVAVERFPEDTFLERVRGSDL
                     VGLEYEGPYDHLEPARDIVHRVIAWDEVSLEEGTGIVHIAPGAGTEDFELAKVHDLPV
                     LVPVDESGRFLPEYGWLHGTSTAEAADQILGDLRERGLLEHSETYVHRYPHCWRCETP
                     LIFRVVDDWFIAVEELRPKLLEANASVEWTPAFYGKRMDDWLRNLGDWNISRKRYFGL
                     PLPIYPCDCGHVNVVGSKAELEERATSGLDQLEELHRPWVDNVPIRCESCAEEVRRIP
                     EVGDVWLDAGIVPFSTLGWQNPEWIPEGYATGAAKGLTRADLPDHAYWEKWFPAHWVT
                     EMREQIRLWFYSLLFMSVVLDGRAPYQKVLGHEDVRDEHGHEMHGSRGNTIEAQEALS
                     RMGADVMRWMYCEQAPTQGVKFGYTPAGEIKRRLLTLWNSVKFFVDYANVASFEPQPG
                     STESSHALDRWLYARTDQLVADMQGAYDRFWTPDATRSFTAFVDDLSNWYIRRSRRRF
                     WDEDAEALQALWHALSRSVQVIAPVMPFLADHLWRVLRADGRPDSVFLEPWPDRGPLD
                     EQLLDEVAEVRSIVELGRQARGEAGLKLRQPLRRMVIQGAQKAEGHAAEIGEELRIKE
                     VEFGPVEATELRVKPNLPLLGPKLGKELGAIRAALLAGDFEQLEGGGVRVNGHEFGAD
                     EILVERGAREGWALAEADSLTVAVTTELDDELRQEGRVYDLTHQLNTMRKEAGLALTD
                     RIRVVLAESDADLLDYEERIKDEVLATEIVVGRVAEPQIEKI"
     gene            complement(3594..4691)
                     /locus_tag="VF225_03375"
     CDS             complement(3594..4691)
                     /locus_tag="VF225_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7144324.1"
                     /translation="MREVPDRIQRLPDQYFAALLARVAAAALAGGGPLVDLGRGNPEV
                     GPPEHVVRALAESAERNDVHGYAPFAGVPALKEAIAVRYGEVYGVHLDPAREVAVLPG
                     TKTGLMEFALCAVESGGTIVLPDPGYPDYFSAVALAGANHVPFTDFDVAADAMYLNFP
                     SNPTAATVPDGLFAEAVEWAERSEAWVLHDFAYGDLVFDGRRPQSFLAVEGARRVGIE
                     LFSMSKTYGMAGWRLGFALGNAELVRRIELLQDHALAGIFRPIQEAGIAALTGPQDSV
                     EERRATYERRRDRALATLEGIEAQSEGTFFVWFRLPEGVTVDQLLVEHRVAVAPGEGF
                     GARGAGWARLSLAVTDEILDRGLERVRAALA"
     gene            complement(5096..>5546)
                     /locus_tag="VF225_03380"
     CDS             complement(5096..>5546)
                     /locus_tag="VF225_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144325.1"
                     /translation="TLSVDETTEIIDVLKRRFPQIHGPQREDICYATSNRQWAVKEML
                     NEIDLLLVIGSRNSSNSNRLVEVASLAGVNAHLIDDETEIDERWFDDVQTVGLTSGAS
                     APERLVARVCDWFRAQGVSEISSFSSVYEDVVFRLPVELRRAETSAA"
BASE COUNT          931 a         1855 c         1886 g          874 t
ORIGIN      
        1 agtgctggtc ctcggtggtt ggctcggtgg ctcaatcgtc ttcgtccacg ggatgcgcgt
       61 gctgaacctt gtggacgagc cggccatgcg cgcagccgcg ccggtcgaat acccggagaa
      121 ggaggaggcg gaggggagct agctagctct cgtcgacgag gccgaggctc ttctcgatcg
      181 cgcccttcgg ctgggcgccg atcgcagctg cggcgggctc gccgtccttg aagaggatca
      241 tcgtcgggat cgactggacc ccgtagcgct gcgacagcgc cggctgctcg tcgatgttca
      301 ccttcacgag cttgaggtcg ccgttccgtt cgtcgacgat ccggtcgagc accggtgcga
      361 cggcgtggca gggcccgcac cattcggccc agaagtcgac gatcaccggc ttctcgctct
      421 ggagcacttc ctcttcgaag ctctgttcgg tcactgcagt tgtcatcgga tcctttctag
      481 cgaatcaatc ggtcgtaccc aggcatcaac gcttcgaatc gcctggttct tccagcccta
      541 caatcggctc cagtgttcga gccgctcccc cagtatccag atcacccggc cctcgaagag
      601 gagatcctcg cctggtggga ggaggcgcgg atcttcgacc ggctacggga gcgaaaccgc
      661 ggcggtcccc actggagctt cttcgacggc ccgaagaccg cgaacacgac cgacggcctg
      721 ggcgtccacc acctctgggg gcgcacctac aaggacgtct tccagcgcta caaggcgctc
      781 cagggcttcg accagcgcta ccagaacggg ttcgactgcc agggcctctg ggtcgaggtc
      841 aaggtcgagc gcgatctcgg gctcaactcg aagcccgaga tcgagcagta cgggatggcc
      901 gcattcgcgc gccgctgccg tgagctcgtc gccgaatccg cgcgggtcat caccgagcag
      961 tcgatccggc tcggacagtg gatggactgg gggaacgact actacacctt cagcgacacg
     1021 aacatcgagt acatctggcg gttcctgaag acgctggatg agcgcggctg gctctatcag
     1081 ggccaccgct cgaccgaatg gtgtccccgc tgcggcacct cgatctcgca gcacgagctc
     1141 tcccagtcgg aggtctatcg ggacaagtcg gatccgtcgc tcttcgtccg tttcccgctg
     1201 aaaggacggg accgggagtc gctagtcgtc tggacgacca cgccgtggac gctgccggcg
     1261 aatgtcgcgg ccgcggtcaa tcccgacgcc gagtacggca agcaggacac gggggagtgg
     1321 gtcgcggtcg aacgctttcc cgaggacact ttcctggagc gtgtgcgcgg ctccgacctc
     1381 gtcgggctcg agtacgaagg cccctacgat cacctcgagc ctgcgcggga catcgtccac
     1441 cgcgtgatcg cctgggacga ggtctcgctg gaagagggga cgggcatcgt gcacatcgca
     1501 ccgggcgccg gcaccgagga cttcgagctc gccaaggtgc acgacctacc ggtgctcgtc
     1561 ccggtcgacg aaagcggacg cttcctgccc gaatacgggt ggctgcacgg aacctcgacc
     1621 gccgaggccg ccgaccagat cctcggcgat ctgcgcgagc gagggctgct cgagcactcc
     1681 gagacgtacg tgcaccgcta tccgcactgc tggcgctgcg agacgccgct gatcttccgc
     1741 gtcgtcgacg actggttcat cgccgtcgag gagctccggc cgaagctgct cgaggcgaac
     1801 gcgagcgtgg agtggacgcc tgccttctac ggcaagcgca tggacgactg gctgcggaac
     1861 ctgggcgact ggaacatctc gcgcaagcgc tacttcggct tgccgttgcc gatctacccg
     1921 tgcgactgcg gccacgtgaa cgtggtcggc tccaaggcgg agctcgagga gcgcgccacc
     1981 tcggggctcg accagctcga ggagctacac cgcccctggg tcgacaacgt gccgatccgc
     2041 tgcgagtcgt gcgctgagga ggttcgacgc atacccgagg tcggtgacgt ctggctggac
     2101 gccgggatcg tccccttctc cacgctcggc tggcagaacc cggagtggat ccccgagggc
     2161 tatgcgaccg gcgcggcgaa gggtctcacc cgcgccgacc ttcccgacca cgcctactgg
     2221 gagaagtggt tccccgcgca ctgggtgacg gaaatgcggg agcagatccg cctctggttc
     2281 tactcgctgc tcttcatgtc cgtcgtgctc gacgggcgcg cgccgtacca gaaggtgctg
     2341 ggccacgagg acgtgcgcga cgagcacggc cacgagatgc acggctcgcg cggcaacacg
     2401 atcgaggcgc aggaagcgct ttcgcgcatg ggcgcggacg tgatgcgctg gatgtactgc
     2461 gagcaggcgc cgacgcaagg agtgaagttc ggctacacgc ccgccggcga gatcaagcgc
     2521 cgcctgctga cgctgtggaa ctccgtgaag ttcttcgtcg actacgcgaa cgtcgcaagc
     2581 ttcgagccgc agccgggcag cacggagtcg tcgcacgcgc tcgaccggtg gctctacgcg
     2641 cgcaccgacc agctggtcgc ggacatgcaa ggcgcgtatg accgtttctg gacacccgac
     2701 gcgacgcgct cgttcacggc attcgtcgac gatctctcga actggtacat ccgacggtct
     2761 cggaggcgtt tctgggacga ggacgcggag gccctgcagg cgctctggca cgcgctcagc
     2821 cgcagcgtgc aggtgatcgc tccggtgatg ccgttcctcg cagatcatct ctggcgcgtg
     2881 ctgcgcgccg acggccggcc ggactcggtc ttcctcgagc cgtggcccga ccggggcccg
     2941 ctggacgagc agctgctcga cgaagtcgcc gaggtgcgca gcatcgtcga gctcggacgt
     3001 caggcgcgcg gcgaagccgg cctgaagctg cgccagccgc tccgccgcat ggtcatccag
     3061 ggcgcgcaga aggcagaggg tcacgccgct gagatcgggg aggagctgcg catcaaagag
     3121 gtcgagttcg ggcccgtcga ggcgaccgag ttgcgcgtca agccgaacct gcctctactg
     3181 ggacccaagc tcggcaagga gctgggcgcg atccgtgccg cgctgctcgc cggcgacttc
     3241 gaacagctcg agggcggcgg agttcgagtc aacggccacg agttcggagc ggacgagatc
     3301 ctcgtcgaac gcggcgcgcg cgagggctgg gcgctcgccg aggcagactc gctgacggtc
     3361 gcggtgacga ccgagctcga cgacgagctg cggcaggagg gtcgcgtgta cgacctcacg
     3421 catcagctga acacgatgcg caaagaggcg gggctcgccc tcacggatcg gattcgagtc
     3481 gtcctagcgg aatccgatgc cgacctcctc gactacgagg agcgcatcaa ggacgaggtc
     3541 ctggcgaccg agatcgtcgt cggtcgggtc gcggagccgc agatcgaaaa gatctaggcg
     3601 agcgcggcgc gcacccgctc gaggcctcgg tccaggatct cgtcggttac ggcgagcgag
     3661 agtcgcgccc acccggcgcc gcgggcgccg aaaccttcgc cgggtgcgac cgcgacgcgg
     3721 tgctcgacga ggagctgatc gaccgtcacg ccctcgggca gccggaacca gacgaagaac
     3781 gtgccttccg actgcgcctc gatcccctcg agcgtagcga gcgcccggtc gcgacggcgc
     3841 tcataggtcg cgcgacgctc ctcgacggag tcctgcggcc cggtcagcgc ggcgatcccg
     3901 gcctcctgaa tgggccgaaa gattcccgcg agggcatggt cctggaggag ctcgatgcgg
     3961 cgaacgagct cggcgttccc cagcgcgaag ccgaggcgcc agccggccat cccgtaggtc
     4021 ttcgacatgc tgaacagctc gatgccgacg cgccgcgcgc cctcgactgc gaggaagctt
     4081 tgcgggcgcc gtccgtcaaa gaccaggtcg ccgtacgcga agtcgtgcag aacccatgcc
     4141 tcgctgcgct cggcccactc gactgcttct gcgaagagac cgtccggcac agtcgccgct
     4201 gtggggttgg aggggaagtt cagatacatc gcgtccgccg cgacgtcgaa gtcggtaaag
     4261 ggaacgtggt tcgcacccgc gagcgcgacg gcggagaagt agtccggata gccgggatcg
     4321 ggcagcacga tcgtgccgcc gctctcgacc gcgcagagcg cgaactccat caggcccgtt
     4381 ttcgtccccg ggaggacggc gacctcgcgg gcgggatcga gatgcacgcc gtacacctcg
     4441 ccgtaacgga ccgcgatcgc ctccttcaga gcaggcactc ccgcgaacgg cgcgtagccg
     4501 tggacgtcgt tgcgctccgc ggattccgcg agcgctcgca ctacgtgctc gggaggcccg
     4561 acttcaggat tcccccgccc gagatcgacc aacggccccc cgcccgccaa cgccgcagcc
     4621 gcgactcgcg cgagcagggc cgcaaagtat tggtccggca accgctggat ccgatccggg
     4681 acctcgcgca cgcgacgatg ctaagccgct tgtcgtggcg ctgcgttagg cagcagtgac
     4741 ccagggcggg cgttagacgg ccgcatgggc aaagcccacg cggcctctcg ccggcgtcgc
     4801 cgagtcgacg cctatggcag gctacgagcc atggcgcgct gtcagcgctg tggaggggac
     4861 ctagaggagc gtttccgctt ctgtccctgg tgcgcgtcgc ctcagcgcac gaagatcgtc
     4921 gagttctttg caccgcaccc gcgcgatgcc ggcaaggcgt tacgcgtgtc gcgctatctg
     4981 accagagacc cgcacgtgcg cttcagcgtg tggagcgaga cgggcgtcgc cgaaagcgcg
     5041 gtctcgctgc gcgaatcgga agcagcgagg cttgcgcgct tcctcagtcc cgtcgtcagg
     5101 cagccgacgt ctctgctcga cgcagctcga cgggtagccg gaacacgacg tcctcgtaga
     5161 cggacgagaa cgagctgatc tcgctcacac cctgagcgcg gaaccagtcg cagacacgag
     5221 ccacgagccg ctccggcgcc gaggcgcctg aggtcagccc gaccgtctgc acgtcgtcga
     5281 accagcgctc gtcgatctcc gtctcgtcgt cgatcagatg cgcattcaca ccggcgagac
     5341 tcgcgacctc gacaaggcgg ttcgagttgg acgaattgcg cgagccgatt acgaggagga
     5401 ggtcgatctc gttcagcatc tccttgacgg cccactggcg gttcgaggtc gcgtagcaga
     5461 tgtcttcgcg ctgcgggcca tggatctgcg ggaagcgccg cttgagcacg tcgatgatct
     5521 cggtggtctc gtcgaccgag agcgtg
//
LOCUS       DASVEU010000088         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1617784,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000088 DASVEU010000000
VERSION     DASVEU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617784"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..393)
                     /locus_tag="VF225_03385"
     CDS             complement(34..393)
                     /locus_tag="VF225_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX7144326.1"
                     /translation="MEAAPSLSSPLGRISISNEAVAHIVGRVAAEAYGVVGMAARNPR
                     EKLLTRDRLRQGITVGGSADEGVTIELHVIVEYGLNLAEVASTLRNRVQYEVERLTGL
                     PVAEVDVRIQDVKRSTA"
     gene            complement(433..624)
                     /gene="rpmB"
                     /locus_tag="VF225_03390"
     CDS             complement(433..624)
                     /gene="rpmB"
                     /locus_tag="VF225_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7144327.1"
                     /translation="MSKVCAVCGKKPGFGNNRSHSMVATKRRFNPNLQRVRVLIKGKA
                     TRAYVCTRCLKGGKVQKAI"
     gene            complement(813..1448)
                     /gene="ribH"
                     /locus_tag="VF225_03395"
     CDS             complement(813..1448)
                     /gene="ribH"
                     /locus_tag="VF225_03395"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7144328.1"
                     /translation="MSERWPSQPTKVDEPENDVVDEEKLPAAEVAEEELPQLRAPDEQ
                     HAYGELDIPDDVNVIEGEPDGTRRGVAIVAARFNGEITNKLLDAALGELDEAGVRRDA
                     ITVMPVPGAFELPLAAMALAKTRRFACVVALGCIVRGETPHFEFIASEAASGLQLAAL
                     ETGVPVAFGVLTVDMIEQAEARVSKGAEAVRTALEMADAFSQLRASASRLT"
     gene            complement(1445..>2410)
                     /gene="ribA"
                     /locus_tag="VF225_03400"
     CDS             complement(1445..>2410)
                     /gene="ribA"
                     /locus_tag="VF225_03400"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7144329.1"
                     /translation="EAREGVTTGISAPDRSRTIQVAVDPDAKPEDLVQPGHVFPLRAC
                     DGGVLARAGQTEAAVDLARLAGLIPAGVVCEIMNEDGTMARVPDLIPYCARHGLKLIT
                     TADLIEYRRRHERLVERMTTVAMPTVYGDFTAVAFRETLTGKHHVALVKGDVEGAENV
                     LVRVHSECLTGDVFHSLRCDCGEQLEQALERIAVEERGVLLYMAQEGRGIGLLNKLRA
                     YELQENGYDTVEANLELGFQPDMRDYGIGNQILAELGLTTIRILTNNPKKITGIEGFG
                     LEVVEQVPIETTPNAQNAQYLATKRDKLGHKLHHQDLKYEPLEGE"
BASE COUNT          353 a          840 c          783 g          434 t
ORIGIN      
        1 cgtgcgcgag ctcctgcact cgctcgcggt ctgtcacgcc gtcgacctct tgacgtcctg
       61 gatgcgcacg tcgacttcgg ccacggggag cccggtgaga cgctcgacct cgtactgcac
      121 gcggtttcgc agcgtcgacg cgacctcggc gaggttcagg ccgtactcga cgatgacatg
      181 gagctcgatc gtgacgccct cgtcggcgct gccgccgacc gtgatcccct ggcgtagccg
      241 gtcgcgcgtc agcagcttct cgcgcggatt gcgcgcggcc atgccgacca ccccgtaggc
      301 ctcggccgcg acgcggccga cgatgtgagc caccgcctcg ttggagatcg agatccgccc
      361 cagcggagac gacagcgaag gagctgcctc cacggcggga ttagaccacg acgagccgct
      421 ggaagttgct gctcagatgg ccttttgcac cttgccgccc ttgaggcagc gggtgcagac
      481 gtatgcccgg gtcgccttgc ccttgatgag cacgcggacg cgctggaggt tggggttgaa
      541 gcggcgcttg gtggccacca tcgagtggct ccggttgtta ccgaagccgg gcttcttccc
      601 gcagactgcg cagaccttgg acatcggccg ggcagtgtag ccaacgagcc caaaacggct
      661 ggggtcagac ccttgccgtt cgagacgtgg ctttgacggg gcgtgctcct aacggctggg
      721 gtcagaccct tgccgttcga gacgtggctc tgacgggcgt gctcctaaca gctggggtca
      781 gacccttgca gttcgagacg tggctctgac gatcaggtca gccgcgacgc ggaagcccgc
      841 agctgcgaga aagcgtcggc catctcgagc gcggtccgca ctgcttccgc gcctttcgag
      901 actcgggctt ccgcctgctc gatcatgtcc accgtcagga cgccgaacgc gacgggtacg
      961 cccgtctcca gagcagccag ctggaggccg ctcgccgctt cggaggcgat gaactcgaaa
     1021 tgcggggtct cgccgcggac gatgcagcca agcgccacca cgcacgcgaa ccggcgcgtc
     1081 ttcgcgaggg ccatcgccgc gagcggtagc tcgaaggcgc cgggaaccgg catgaccgtg
     1141 atcgcgtcac gacgcacgcc cgcctcgtcg agctccccga gcgccgcgtc gagcagcttg
     1201 ttcgtgatct cgccgttgaa ccgcgcggcg acgatcgcga caccgcgtcg cgtcccgtcc
     1261 ggctcccctt cgatgacgtt cacgtcgtcc gggatgtcga gctcgccgta cgcatgctgc
     1321 tcgtccggcg cgcgcaactg cggcagctct tcctccgcga cctcggcggc cggcagcttc
     1381 tcctcgtcga ccacgtcgtt ctccggttcg tccaccttcg tcggttgact cggccagcgc
     1441 tcgctcattc gccctccagg ggctcgtact tcaggtcctg gtgatgaagc ttgtggccga
     1501 gcttgtcacg tttcgtggcg aggtactgcg cgttctgcgc gttcggcgtg gtctcgatcg
     1561 gcacctgctc caccacctcc agcccgaagc cctcgatgcc ggtgatcttc ttcgggttgt
     1621 tcgtgaggat gcggatcgtg gtcaggccga gctcggcgag gatctggttg ccgatcccgt
     1681 agtcgcgcat gtccggctga aacccgagct cgaggttcgc ctccaccgtg tcgtagccgt
     1741 tttcctggag ctcgtaggcg cgcagcttgt tgaggagacc gatgccgcga ccttcctgtg
     1801 ccatgtagag cagcacgccg cgctcctcga cggcgatccg ttcgagtgcc tgctcgagct
     1861 gctcgccgca gtcgcagcgc aacgaatgga agacgtcgcc cgtcagacac tcggagtgca
     1921 cgcgcacgag cacgttctcg gcgccctcga cgtcgccctt cacgagcgcg acgtggtgct
     1981 tgccggtcag cgtctcgcgg aacgcgacag ccgtgaagtc gccgtacacg gtcggcatcg
     2041 cgaccgtcgt catgcgctcg acgagccgct cgtgccgccg cctgtactcg atcaggtccg
     2101 ctgtggtgat cagcttcagc ccatggcgtg cgcaatacgg gatcagatcc gggacgcgag
     2161 ccatcgttcc gtcctcgttc atgatctcgc agacgacgcc cgcgggaatc aagcccgcaa
     2221 gccgcgccag gtcgaccgcc gcctccgtct ggcctgcgcg ggcgagtaca ccgccgtcgc
     2281 acgcacgcaa cgggaacaca tgcccgggct gcacgagatc ttccggtttc gcgtcgggat
     2341 cgacagcgac ctggatcgtg cgcgaacgat ccggcgccga gatgccggtc gtcactccct
     2401 cgcgcgcctc
//
LOCUS       DASVEU010000089         4690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_839420,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000089 DASVEU010000000
VERSION     DASVEU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839420"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VF225_03405"
     CDS             complement(<1..1189)
                     /locus_tag="VF225_03405"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEX7144330.1"
                     /translation="MLDDLLSRELDRFEREHPRSRELAARAEHSLLAGVPMPWMIRWA
                     GGFPVFAAEAHGARFRDVDGLEYVDFCLGDTAAMTGHSPAPTVRAVAEQAARGITLML
                     PSEDSLWVGEELARRFGLPSWQFALTATDANRFAIRLARELTGRPKILVFNWCYHGTV
                     DETFAALDGASVVAREGNIGPPVALEETTRVVEWNDVEALERELGFGDVACVLAEPAL
                     TNIGIVHPAPGFHEALRAATRKTGTLLIIDETHTLCAGPGGYTAAYGLDPDLLTVGKA
                     IAAGIPAAAYGFSREVADQLEALLPRDERADVGGIGGTVAANVLSFAAMRATLAEVLT
                     NEAFARMIALGERFEAGVAGAIERHGLPWHVTRLGCRVEYLFRPERPRNGSEAAAGGN
                     AELD"
     gene            1297..2769
                     /locus_tag="VF225_03410"
     CDS             1297..2769
                     /locus_tag="VF225_03410"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7144331.1"
                     /translation="MDKDLVADTAAAERLGTALRRAGYSEDAIHRLLGEDAYSSERAD
                     TPAEERRLPNTPLATLVRLLFLQLPVSVQDAEAALGRESVEALEATGLAEVGDRVRSR
                     ARILPIGKVLLASDGFSRDGDDPADYVATYTPTARVLDSLTPRPRVDRALDVGTGSGI
                     HALLAAQHARHVIATDVNPRAIAYTELNAALNGLRNVECRRGSLFEPVEGETFDLITC
                     NAPYVVSPERRWVYRDSEFSEDEVSERVILAAAEHLTAGGYAAMLVSWLAFDENSPDE
                     RVLAWADASGCDSWILSTLNSDPITHAAEWNSHLAGEALGDALDEWTQYFDSLGVQSV
                     SEGAVVLHKRPDGGHAARVDELDEDELDDAGDQILRAFASRAKLAGLGGDKELLETRI
                     GVASTLRLDRELEPSGGRAVDVDGSVELTEGTKHAVDASADVQEVVASLDSRMRLSEV
                     IDTTADRLGLLDDEVATIREEALDVTRELLELGALRFRGD"
     gene            2793..3272
                     /locus_tag="VF225_03415"
     CDS             2793..3272
                     /locus_tag="VF225_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX7144332.1"
                     /translation="MKIATIGIYGWTPESFLAHLRAANVRVVFDVRQRRGVRGPEYAW
                     ANSLRIQAALDNAGIDYRHHQELAPTTELRHVQYEADARHGVGKRSRMELAPEYRERY
                     LREILDRVDLGELVHALPAEGTTALMCVERDPEACHRSLITERLEAEHGISVVHLRP"
     gene            3476..4150
                     /locus_tag="VF225_03420"
     CDS             3476..4150
                     /locus_tag="VF225_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144333.1"
                     /translation="MSPVLVVDVGGSHVKVLASEEPEPRRADSGPDLTAAQMVDSAKK
                     LAADWTWDSVSVGVPSPVHGGRVAAEPVNLGAGWVGFDYAEAFGKPTKVVNDAAMQAL
                     GSYEGGRMLFLGLGTGLGSALVTDGFVQPMELGHLPYKKATFEDYVGSAGLGKRGKRR
                     WRLDVADVVAKLSAALGPEYVVLGGGNAKKLDPLPPNARLGSNQNAFIGGFRLWDPDA
                     TARILS"
     gene            complement(4464..4622)
                     /locus_tag="VF225_03425"
     CDS             complement(4464..4622)
                     /locus_tag="VF225_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144334.1"
                     /translation="MNTGQEVVLAVVAVATILLVLGLFVWAAIKDGQEDKALQKRLRI
                     RRRTRLGR"
BASE COUNT          783 a         1623 c         1570 g          714 t
ORIGIN      
        1 ggtcgagctc ggcgttcccg ccggcagccg cttcgctccc gttccgcggt cgctcgggac
       61 ggaagagata ctcgacccgg cagccgagac gcgtgacatg ccacgggagg ccgtgccgct
      121 cgatcgcacc ggcgacgccc gcttcgaagc gctcgccgag ggcgatcatg cgcgcaaacg
      181 cctcgttggt gagaacttcg gcgagggtgg cacgcatcgc agcaaacgac agaacgttcg
      241 cggcaaccgt gccgcctatg cctccgacgt cggcgcgctc gtcgcgtggc agcagcgcct
      301 cgagctggtc cgccacctcg cgcgagaacc cgtaggcggc ggcgggaatg cccgcggcga
      361 tcgccttccc taccgtcaac aggtccgggt cgagcccata ggcggcggtg tagccgcccg
      421 gtcccgcgca cagcgtgtgc gtctcgtcga tgatcagcag cgttcccgtc ttgcgggtcg
      481 ccgctcgaag agcttcgtgg aacccaggcg cgggatggac gatcccgatg ttcgtcaacg
      541 caggctccgc cagcacgcac gcgacgtcgc caaagccgag ctcgcgctcg agggcctcga
      601 cgtcgttcca ctccaccacc ctcgtcgtct cctcgagcgc gaccggcggc ccgatgttcc
      661 cctcacgcgc gaccaccgac gcaccgtcga gcgcagcgaa cgtctcgtcg acggtgccgt
      721 ggtagcacca gttgaagacg aggatcttcg gccggcccgt gagctcgcgc gcgagcctga
      781 tcgcgaaccg attcgcgtcc gtcgcggtga gtgcgaactg ccagctcggc agcccgaagc
      841 gacgcgccag ctcttcgccg acccaaaggg aatcctcact cgggagcatg agcgtgatgc
      901 cgcgtgcggc ctgctctgcc accgcccgca cggtcggtgc cggggaatgc cccgtcatcg
      961 cggccgtgtc accgaggcag aagtccacat actcgaggcc gtccacgtcg cgaaaacgcg
     1021 ccccgtgcgc ctcggcggcg aacacaggga agcctcccgc ccagcggatc atccacggca
     1081 tcggcactcc cgcgagcagc gagtgctcgg cccgcgcggc gagctcgcgc gaacgcgggt
     1141 gctcacgttc gaaccggtcg agctctcgtg acaggagatc gtcgagcaac gttcccaagg
     1201 gtggttgtct ggtcccgctc gtcacggtgc tagtcaatca agctcgaaac gcgcttgcgg
     1261 tccgttcgat tcgggcatga tgattccgct ttggtgatgg acaaagatct cgtggccgac
     1321 accgcagcgg ccgagcgtct cggcacggct ctgcgtcgtg ccggctattc ggaggacgca
     1381 atccaccgac tgctcggtga agacgcttac tcgagcgagc gcgccgacac gcccgccgag
     1441 gaacgtcgct tgccaaacac accgctcgcg acgctggtgc ggctgctctt tctccagctg
     1501 ccggtttcgg tgcaggacgc cgaggccgca ctcggccgag agagcgtcga ggcgctggag
     1561 gcgacagggc tcgcggaggt cggcgaccgg gtcagatcgc gtgcccggat cctgccgatc
     1621 ggcaaagtcc tcctcgcctc ggacggcttc tcccgcgacg gcgacgaccc ggccgactac
     1681 gtcgcgacct acacgccgac ggctcgcgtt ctcgattccc tgacgcctcg tcctcgtgtc
     1741 gaccgggcgc tggacgtcgg cacggggagc ggcatccacg ccctgctcgc ggcgcaacat
     1801 gcccggcacg tgatcgccac cgacgtcaac ccacgggcga tcgcgtacac ggagctcaac
     1861 gcagcgctga acgggcttcg caacgtcgag tgccgccggg gcagcctctt cgagcccgtg
     1921 gaaggcgaga cgttcgacct gatcacgtgc aacgcgccct acgtcgtctc gccggaacgt
     1981 cgctgggtct accgcgacag cgagttctcc gaggacgagg tgtcggagcg cgttatcctc
     2041 gccgcggccg aacacctgac cgcaggcggc tacgcggcga tgctcgtgag ctggctcgcg
     2101 ttcgacgaaa acagtcctga cgagcgcgtg ctcgcgtggg cagacgcgag cggttgcgac
     2161 agctggattc tctccacgtt gaactccgat ccgatcacgc acgccgccga atggaattcg
     2221 caccttgcag gcgaagcgct cggcgacgct ctggacgaat ggacgcagta cttcgactcc
     2281 ctcggggtgc agtcggtgag cgagggcgcc gtggtcctgc acaagcgccc cgacggcgga
     2341 cacgctgccc gggtggatga gctcgacgag gacgaactgg acgatgcagg cgatcagatc
     2401 ttgcgcgcgt tcgcatcgcg agcgaagctt gccgggctcg gcggggacaa ggagttgctg
     2461 gaaacacgca tcggtgtcgc ctcgaccctg cgacttgacc gcgagctcga gccctcaggt
     2521 gggcgtgccg tcgacgtcga cggcagcgtc gagctgactg aggggacgaa gcacgcggtc
     2581 gacgcgtccg ccgatgtcca agaggtggtc gcctccctcg acagccgcat gcgcctgagc
     2641 gaagtcatcg acaccaccgc cgaccggctc ggcctgctgg acgacgaggt ggcaacgatt
     2701 cgcgaggaag cgctcgacgt cacccgcgag ctcctcgagc tcggcgcgct gcgcttccgc
     2761 ggcgactgac gacgcgccgg gctgccgggt cgatgaagat cgccacgatc gggatctacg
     2821 gctggacgcc cgagagcttt ctcgctcatc tgcgcgccgc aaacgtgcgg gtcgtattcg
     2881 acgtgcgcca gcgccgcggt gttcgcggcc cggagtatgc ctgggccaac tcgctccgca
     2941 tccaggccgc tctcgacaac gccgggatcg actaccggca ccatcaggag ctcgcgccga
     3001 ccaccgagct ccggcacgtg cagtacgagg cggacgcgcg gcacggtgtc gggaagcgct
     3061 cccgcatgga gctggctccc gaataccgcg agcgctacct ccgtgagatc ctcgaccggg
     3121 tcgatctcgg cgagctcgtc cacgcgttgc cggccgaggg cacgacggcg ctgatgtgcg
     3181 tcgaacgtga tcccgaggcc tgccatcggt cgctgatcac cgagcgtctc gaagccgaac
     3241 acggcatctc tgtcgtgcac ctacgcccct gaggcactgg cagtcgcgcc catcggccgg
     3301 tcgacgtagt ctttacgaca ctggagtcaa gatcgatctg gaggtcacga tgaaaggcgc
     3361 ctcgaagccc aagaagctgg gcaagaaagt agcccagaag tccctgaagg agaagcggca
     3421 ggacaaacgc gccgcagcaa agaagggctt cggctcgagc tagctcaggt cgatcgtgag
     3481 cccggtgctg gtcgtcgacg tcggcgggtc tcacgtcaag gtgctcgcca gcgaggagcc
     3541 ggagccccgg cgggccgatt cagggccgga tctgaccgcc gcgcagatgg tcgactcggc
     3601 gaagaagctg gcggccgact ggacgtggga cagcgtttcc gtgggcgttc ccagcccggt
     3661 ccacggtgga cgcgtcgccg cagagcccgt caatctcggc gcaggctggg tcggcttcga
     3721 ctatgccgag gcattcggga agcccacgaa ggtcgtcaac gatgccgcga tgcaggcgct
     3781 cggcagctac gagggcggca gaatgctctt cctcgggctg ggaactgggc tcgggtccgc
     3841 gctcgtcacg gacggtttcg tccagccgat ggagctcggg caccttccct acaagaaagc
     3901 gacgttcgag gattacgtcg gcagcgccgg cctcggcaag cgaggcaaga ggcgttggcg
     3961 ccttgacgtc gcggacgtcg tcgccaagct gagcgcggca ctggggccgg agtacgtcgt
     4021 gctcggcggc ggtaacgcga agaagctcga tccactgcca ccgaatgcgc ggctcggcag
     4081 caaccagaac gcgttcatcg gcggtttccg cctgtgggac ccggatgcga ctgcgaggat
     4141 ccttagttag gggcgtcgac cgcggctcac accgctccgg aggccgccac gcccgcggga
     4201 cgcgcgcgtt cccagaggcg ctccccgcac atcatgcagc gcggcagcac ccggcggacg
     4261 atcaccgcgt tgccacaggc ggcgcagagg aaccaaccgc tggtggctac cccggcaggc
     4321 cagaactcca cgtaatcccg cgcgtcatca gcccgaacag cgggctttgt tccgtccccc
     4381 ggctcgtaca cctcaacccc ctcaatcgct gcgagctccc cgctcctgct ccttgtaggt
     4441 cgaaggtgca gcggtgtcaa ccgctaacgc ccgagcctcg tccggcggcg aatccgcagg
     4501 cgcttctgca acgccttgtc ctcctgtcca tccttgatcg cggcccagac gaacagcccg
     4561 aggaccagga ggatcgtcgc tacggcgacg accgcgagga cgacctcctg tccggtgttc
     4621 atggcgcaag tctaggtcgc tcgctccggc tcgtattacg aagtagcggt tgcgtcgacc
     4681 ggcgcgccgg
//
LOCUS       DASVEU010000090         6472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1422696,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000090 DASVEU010000000
VERSION     DASVEU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422696"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VF225_03430"
     CDS             <1..353
                     /locus_tag="VF225_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7144335.1"
                     /translation="FGFLFELMDDYYPAPNTGFVVCDKEGRIIASGRGVFELSGFREQ
                     ELLGADLVERFGLNGFEGNQNPAALALEWGVRRQGERMEMRTRAGQRKAVTADFFPAY
                     DDDGGLLVAITPRS"
     gene            complement(337..1086)
                     /locus_tag="VF225_03435"
     CDS             complement(337..1086)
                     /locus_tag="VF225_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144336.1"
                     /translation="MSELQHPESRSFELVADVYERARPGYPPEAVAWIADRLDLRPGR
                     TVLDLGAGTGKLTRALLETGARVIAVEPGDAMRAELERAVPGAEALRGSAEEIPLPDG
                     SVDAITVGQAFHWFRHELALPEFHRVLPARGGVALVWNARDPEAPLHQEIGKLIASFV
                     PPDNVPSEEWSQQLAESELFGPLEERRFSFVQQLDAGLLVDRIASVSFVAAAPPAARR
                     ELDAELRRVAERLGGRVDFPYLTTAYVSFAV"
     gene            complement(1079..1747)
                     /gene="nth"
                     /locus_tag="VF225_03440"
     CDS             complement(1079..1747)
                     /gene="nth"
                     /locus_tag="VF225_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7144337.1"
                     /translation="MARSFVQEARRRVGPKRARIREIIDRLAAAHPDATIALKSRSPL
                     ELLVAVMLSAQTTDVNVNRVTERLFVKYRKPEDYLAVPPEELERDIFATGFYRQKAKS
                     LRGTMAMLLTEFDGEVPHRLEDLVRLPGVARKTANVVAAELGEPQGIVVDTHVRRLSQ
                     RLGLTKQEDPIKIERDLIKLVPRQDWGRFPHLLIWHGRRVCLARLPRCEECVLNDICP
                     SSRV"
     gene            complement(1750..5109)
                     /locus_tag="VF225_03445"
     CDS             complement(1750..5109)
                     /locus_tag="VF225_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7144338.1"
                     /translation="MNSEQQRAVDARGLVFVSAGAGTGKTKVLVERFARAVCDEGVDV
                     ESILVITYTEKAAGELRSRIRAGLVDRGRPDLARELDGAWISTIHGFCNRLLRSHPFA
                     AGVDPRFRVLDESQGRVLRGEAFSAALTAFCANDDPARLRLLAVYGADGLRRMLTGVY
                     ETLRSAGRELVLELGEHPSLAAQVDEFREAARCLTEDDGSGDTQRETARQALAYLEPD
                     TTADSLFDLGDLRLRGERAASYEEARRRLEQTALDELAAADRDLLQELLAGFATAYQE
                     GKDRESALDFEDLQLRARDLLRDDEAIREKEQLRFRSIMVDEFQDTNRLQCELIDLLS
                     GGPGDHERFFVGDEFQSIYGFRHADVQVFRERREQAGDGVLPLTMNYRSRPEVLAVVN
                     HLFGADFGDEFQPLAASGDFPDPVFGAPVELLVTDKSTYSGTDVHWRRGEARAIARRI
                     RDLIAAGDAAPGEIVLLFAAGTDAEWYEEELRALDVTTYRSTGRGYFGQQQVVDLLAY
                     LRLLRNRYDDEALVSVLASPFVGISNDGLVLLRRAAPKRPLFVALERELPETFPERDA
                     QLLRAFRQRYDRLTAALARLSLERLCERIISEHDYDLAVLARWDGRRRYANLRKLARL
                     ARSYEELRGPDVEGFVRFVRDQEAVGARELEAVAEEEGGDAVRLLTIHAAKGLEFKVV
                     VVADAGRDKAPPASHEILALSDGRFGFRVADPITSKPRGAYAYDAVQETRKAEDRAER
                     LRLYYVAMTRAIDRLIISGSIDPDRTADDTTPIGWVLGRLDAKEEIERADGEPVELER
                     GGARVILRVDRFTTEPEVTAEAPAEDGQLALFDEGGAGVLPALVPALQPLVEIPRPPV
                     HRVRRLSFSALALFDRCSYRYYAERVVGMRPTDERRPMPGSPGLAATEIGDAVHRLLE
                     LVSLEHPLPPDDLVARVRAWYPRVNDDELERIGGFVRSYCDSELARRVATLPGAQPER
                     PFAFEHDGVLLHGRLDVLQLEGERAVVVDYKTNSLAEGTPEEIVEHDYRLQRLVYALA
                     CFRAGAQEVEVVYHFLERPDAVVSTVFRVDRLSELEGELSEAIARINAGEFRPTPSEF
                     ICAGCPALDVICAGPRLRSANYAEHALATVS"
     gene            complement(5195..>6472)
                     /locus_tag="VF225_03450"
     CDS             complement(5195..>6472)
                     /locus_tag="VF225_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7144339.1"
                     /translation="PDHVARDRYLFYTACTRPSRRLYLVREAATDEGSPREPSPFWDE
                     VQAVFPKDDVARWTRRRPLSALTWPLEDAPTDRERLRAVAIRAAEDSVTADAIATANG
                     WDRRLARALRAFDRPTRLTHPQVLAELAAKSTFGVTELERFADCSSIWFLERLIDPKS
                     IDARVDARLRGSVAHQALFKFYSGLPKELGADRVEKDRVEDAVAFLRRCLDHAVEGVR
                     LELTDLQERELRYGLWRDLEAFVREEAAADMPLVPRRFEVLFGSERSAPELQRGLELA
                     EGMTLSGKIDRIDVDPFSARGIVQDYKAGKYAHSAQQIESEKRLQIPLYMLVLRDLVG
                     VEPLGGLYRPLSGERKARGLLRAEAREDGIPGFAKNDYLPEEEFWGKVEGARELAVGI
                     VERIRTGDVRHDPKGGDCPTWCELWSMCRIRRA"
BASE COUNT         1059 a         2221 c         2193 g          999 t
ORIGIN      
        1 gcttcggctt tctcttcgaa ctgatggacg actactaccc ggcgcccaac accgggttcg
       61 tggtctgcga caaggaggga cggatcatcg cgagtggccg gggggtcttc gagctctcgg
      121 gcttccggga gcaggagctg ctcggagccg acctggtgga gcgcttcggc ctgaacgggt
      181 tcgaggggaa ccagaacccc gctgccctcg cgctcgagtg gggcgtgcgg cggcagggcg
      241 agcggatgga gatgcggacc cgggcaggcc agcggaaagc ggtgaccgcc gacttcttcc
      301 ccgcctacga cgacgacggc gggctgctcg tagcaatcac accgcgaagc tgacgtacgc
      361 ggtcgtcaag taagggaagt caacgcggcc gccaagccgc tcagccacac gacggagctc
      421 ggcatcgagc tcgcggcgag ccgccggtgg ggccgcggcg acgaagctga ccgaggcgat
      481 gcggtcgaca agcaggcccg cgtccagctg ctgaacgaac gagaagcgcc gctcttcgag
      541 cgggccgaag agctcgctct ccgcgagctg ctgagaccac tcctctgaag gcacgttgtc
      601 cggcggaacg aaggacgcga tcagcttccc gatctcctga tgaagggggg cctccggatc
      661 gcgagcgttc caaacgagcg ccaccccgcc acgtgcgggg agcacccggt ggaactcggg
      721 aagcgccagc tcgtgccgga accagtggaa cgcctgtccg accgtgatcg cgtccacact
      781 tccgtcggga agcgggattt cctcggccga gccccgcagc gcttcggctc cgggcacggc
      841 gcgctccagc tccgcacgca ttgcgtcacc gggctcgact gcgatgacgc gggcgcccgt
      901 ctccagaagc gcgcgggtga gcttgccggt gccggcgccg aggtcgagca cggtgcgtcc
      961 cggccgcagg tcgagccgat cggcgatcca ggccacggcc tcgggcggat aacccggccg
     1021 ggctcgctcg tagacgtcgg cgactagctc gaagctgcgt gactccggat gctggagctc
     1081 agacacgtga cgacgggcag atgtcgttca ggacgcactc ctcgcaacgc ggcagccgcg
     1141 cgaggcagac tcgccgccca tgccagatca gcaggtgcgg gaagcggccc cagtcttggc
     1201 gcggcacgag cttgatcagg tcgcgctcga tcttgatcgg atcctcttgc ttggtgaggc
     1261 cgaggcgttg cgagagccgg cgcacatgtg tgtcgaccac gatgccctgg ggttctccga
     1321 gctcggcggc gacgacgttc gccgtcttgc gggcgacgcc tggcaggcgg acgaggtcct
     1381 cgaggcggtg cgggacctcg ccgtcgaact ccgtcaacag catcgccatc gtgccgcgca
     1441 acgacttggc tttctggcgg tagaacccgg tcgcgaagat gtcgcgctcg agctcctcgg
     1501 gcggaacggc gaggtagtcc tccggcttgc ggtacttgac gaacagcctc tccgtcacgc
     1561 gattgacgtt cacgtcggtc gtctgcgccg acagcatcac ggcgacgagg agctcgagag
     1621 gtgagcgcga cttgagcgcg atcgtcgcgt cagggtgcgc ggcggcgagc cggtcgatga
     1681 tctcacggat tcgggcacgc ttcgggccga cgcgccggcg cgcctcctgg acgaagcttc
     1741 tggccaagac taggagacgg tagcgagtgc gtgctcggcg tagttcgccg accgcaggcg
     1801 cgggcctgcg cagatcacgt cgagtgccgg gcagccggcg cagatgaact cgctcggcgt
     1861 gggacgaaac tcgcccgcgt tgatccgcgc aatcgcctcg ctcagctcgc cctcgagctc
     1921 ggaaagccga tcgacacgga acacggtcga gacgactgcg tccgggcgtt cgaggaagtg
     1981 gtacacgacc tcgacctcct gcgcgccggc gcggaagcag gcgagcgcgt agacgaggcg
     2041 ttgcagccgg tagtcgtgct cgacgatctc ctctggcgtg ccctctgcca gcgagttcgt
     2101 cttgtagtcg acgaccactg cgcgctcacc ctcgagctgg agcacgtcga gccgtccgtg
     2161 cagcaggaca ccgtcgtgct cgaacgcgaa cggccgctcg ggttgcgcgc ctggaagcgt
     2221 cgccacgcgt cgcgcgagct cagagtcgca gtacgagcgg acgaacccgc cgattcgctc
     2281 cagctcgtca tcgttcacgc gcggatacca ggcgcgcacg cgtgcgacga gatcgtcggg
     2341 cgggagcgga tgttccaggc tcaccagctc gagcaggcgg tggacggcgt cgccgatctc
     2401 ggtcgcggcc agcccgggcg agcccggcat ggggcgccgt tcgtcggtcg gcctcatgcc
     2461 gacgacacgt tcggcgtagt agcggtacga gcaacggtcg aacagcgcga gcgcactgaa
     2521 tgacaggcgc ctgactcgat gaaccggcgg cctgggaatt tcgacgagcg gctgcaatgc
     2581 cggcacgagc gctggcaaca cgcccgcacc gccctcgtcg aacagcgcga gctgtccgtc
     2641 ttctgcaggc gcctcggctg tgacttccgg ctctgtcgtg aagcggtcga cgcgcaggat
     2701 cacccgtgca cctccacgct cgagctccac gggctcgccg tcagcgcgct cgatctcctc
     2761 cttcgcatcg agacggccga gcacccagcc gatcggcgtg gtgtcgtcgg ccgtccggtc
     2821 gggatcgatc gagcccgaga tgatcaggcg gtcgatcgcg cgcgtcatgg cgacgtagta
     2881 aaggcgcagt cgctccgcac ggtcctccgc cttgcgcgtt tcctggacgg cgtcgtacgc
     2941 atacgcgccg cgcggcttgc tcgtgatcgg gtcggcgacg cggaagccga agcggccgtc
     3001 ggagagagca aggatctcgt gagacgccgg cggcgccttg tcgcggcccg cgtccgccac
     3061 gacgacgacc ttgaactcga ggcccttcgc tgcatggatc gtcagcagcc ggacggcgtc
     3121 tccgccttcc tcttccgcca ccgcttccaa ctcgcgcgcg ccgacggcct cctggtcgcg
     3181 gacgaaccgc acgaagcctt ccacgtcggg gccgcgcagc tcttcgtacg agcgcgcaag
     3241 cctggcgagc ttccgcagat tcgcgtagcg acgccggccg tcccagcgcg cgagcactgc
     3301 gaggtcgtag tcatgctccg agatgatccg ctcgcacagc cgctcgagcg aaaggcgcgc
     3361 caaagccgcc gtcaggcggt cgtagcgctg gcggaacgca cgcaggagct gggcgtctcg
     3421 ttctggaaag gtttccggga gctcgcgctc gagcgcaacg aacagcggcc gcttcggcgc
     3481 cgcgcgccgc aagaggacga gcccgtcgtt cgagatgccg acgaacgggg atgcgagcac
     3541 gctgacgagc gcttcgtcgt cgtacctgtt ccgcagcagg cgcaagtaag cgagcagatc
     3601 gacgacctgc tgctggccga agtacccgcg tcctgtgctg cggtaggtcg tgacgtcgag
     3661 cgcgcgcagc tcctcctcgt accactcggc atccgttccc gccgcgaaca gtagaacgat
     3721 ctccccagga gcggcgtcgc cggcggcgat cagatcccgg atccgacggg cgatcgcgcg
     3781 cgcctcgccg cgccgccagt gcacatcggt tccggagtac gtcgatttgt ccgtcacgag
     3841 cagctcgacc ggagcgccga agaccggatc ggggaagtcg cctgaagctg ccagcggctg
     3901 gaactcgtcg ccgaagtccg caccaaagag gtggttgacg acggcgagca cctccgggcg
     3961 cgagcggtag ttcatcgtca gcggcagcac gccgtcgccg gcctgctcgc gccgctcgcg
     4021 gaagacctgt acgtcggcgt gacggaaccc atagatcgac tggaactcgt cgccgacgaa
     4081 gaaccgctcg tggtcaccag gtccgccgga aagcaggtcg atcagctcgc actgcaaccg
     4141 gttcgtgtcc tgaaactcat cgaccatgat cgaccggaag cgcagttgct ctttctcacg
     4201 gatcgcttcg tcgtcacgca agaggtcacg tgctcgcagc tgcaggtctt cgaagtcgag
     4261 cgcggattcg cggtccttgc cctcctggta ggcggtggcg aaaccggcga gcagctcctg
     4321 cagcagatct cgatcggccg ccgcgagctc gtccagcgcc gtttgctcca gacggcgacg
     4381 cgcctcctcg tagcttgcgg cccgctctcc acgcagcctc agatcgccga ggtcgaagag
     4441 actgtccgcc gtcgtatcgg gctcgagata agcgagcgcc tgccgcgcgg tctcgcgctg
     4501 ggtgtcgccg gagccgtcgt cttccgtgag gcagcgagcc gcttcgcgaa actcatccac
     4561 ttgagcggcg agactcgggt gctcgccgag ctcgaggacg agctcacgac cggccgatct
     4621 cagcgtctcg tagacgccgg tgagcatgcg gcggagcccg tctgctccgt acacggcgag
     4681 cagcctcagc cgggccgggt catcgtttgc gcagaaagcg gtcagagctg cggagaaagc
     4741 ctcgccgcgc aagacgcgtc cctggctctc gtcgagcacc cgaaagcgcg ggtcgacccc
     4801 ggccgcaaag ggatgagacc ggagcaggcg gttgcagaag ccgtgaatcg tcgagatcca
     4861 cgcaccgtcg agctcgcgcg cgagatcggg gcgcccccgg tcgaccaggc ctgcgcggat
     4921 cctgctgcgc agctcccccg ccgccttctc ggtgtacgtg atcacgagga tcgactcgac
     4981 gtcgacaccc tcgtcgcaca cggcgcgcgc gaagcgctcg acgagcacct tcgtcttccc
     5041 ggtgcccgcg ccggcagaga cgaacacgag cccgcgcgcg tcgactgctc tttgttgttc
     5101 cgaattcaag gcaatgtctt tcagcttcgt ctcccgagcg aagcgaaggg actaactcct
     5161 cgccgcggaa aatgcgcagc atttttcgcg gtaatcatgc ccgccggatc cggcacatgg
     5221 accacaactc acaccacgtc gggcagtcgc cgccctttgg atcgtgccgc acgtcgccgg
     5281 tgcgaatccg ttcgacgatc ccgaccgcga gctcgcgcgc tccttcgacc ttgccccaga
     5341 actcctcctc cgggagatag tcgttcttcg cgaagccggg aatgccgtcc tcacgcgcct
     5401 ctgcgcgcag cagcccgcgc gccttgcgct cgccggagag cgggcgatag agaccgccca
     5461 gcggctcgac gccgacgagg tcgcgcagca cgagcatgta aagcggaatc tgcaggcgct
     5521 tctcggactc gatctgctgc gcggagtgcg cgtacttccc cgccttgtag tcctggacga
     5581 tcccgcgcgc gctgaacgga tcgacgtcga tgcggtcgat ctttcccgag agggtcatcc
     5641 cctcggcgag ctcgagcccg cgctggagct cgggcgcgga acgctccgat ccaaacagga
     5701 cctcgaaccg gcgcggaacg agcggcatgt ctgcggcggc ctcttcgcgg acgaacgcct
     5761 cgagatcgcg ccacaagccg tagcgaagct cacgctcctg cagatcggtg agctcgagcc
     5821 ggacaccctc caccgcgtgg tcgaggcagc gccgtaggaa ggcgaccgcg tcctcgacgc
     5881 gatccttttc gacccgatcc gcgccgagct ccttcggcag tccggaatag aacttgaaca
     5941 gcgcctggtg cgcgaccgac ccgcgcagtc gtgcgtcgac ccgcgcatcg atgctcttcg
     6001 gatcgatcag acgctcgagg aaccagatcg aggagcagtc cgcgaaccgc tccaactcgg
     6061 tgacgccgaa tgtcgacttc gccgccaact cggccaggac ctgcggatgc gtcagtcgtg
     6121 tcggcctgtc gaacgctcgc agggcgcgag cgagccgccg gtcccagccg tttgccgttg
     6181 cgatcgcgtc ggccgtcacg ctgtcctcgg cggcacgaat ggcgaccgcc cgcagacgtt
     6241 cccggtccgt gggtgcgtct tccaacggcc acgtgagcgc ggacagcggg cggcgccgcg
     6301 tccagcgcgc gacgtcgtcc ttcgggaaga cagcttgcac ctcgtcccag aacgggctcg
     6361 gctcccgcgg gctgccttca tcggttgcgg cctcgcgcac gaggtacagc cgacggctcg
     6421 gtcgggtgca agcggtgtag aagagataac ggtcccgagc gacgtggtcg gg
//
LOCUS       DASVEU010000091         8043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_839895,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000091 DASVEU010000000
VERSION     DASVEU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839895"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VF225_03455"
     CDS             <1..676
                     /locus_tag="VF225_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144340.1"
                     /translation="AQEEITWGVFETPEAELNVLGEVSSLDIVELGCGTAYFSAWLAR
                     RGARVVGVDPTPAQLETARRLQRETGIEFPLIEAIGEDVPLPDSSFDLVLSEYGASLW
                     ADPYRWIPEAARLLRPGGRLIFLCNSIISALCMTLEGVGEQLVRPQFALLKIEWPDTG
                     EVEFQLGHGDWIDLMRVNGFEIERLVELFASPDSETHSYYKYVTPDWARKWPAEELWV
                     AELHDG"
     gene            669..1265
                     /locus_tag="VF225_03460"
     CDS             669..1265
                     /locus_tag="VF225_03460"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7144341.1"
                     /translation="MADAPPSPPLLLASTSPQRKAILEQLHVPFDVVAPNYDEAIPKG
                     ADPMQVVREHARGKARSVADVAEDRPVVGVDTAVVLNGRIFGKPANAADAEEMLEELS
                     GKTHVVVSGLCLLTPGWEDVEHEATQVAFRELPPRELGIYVATGEWEGRAGGYAIQGR
                     GAALVRSIEGDYLNVVGLPAALLVRLLAERFSGVYGFG"
     gene            1284..2339
                     /locus_tag="VF225_03465"
     CDS             1284..2339
                     /locus_tag="VF225_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7144342.1"
                     /translation="MGLFDNLGGFGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAID
                     QRTGEVHAVGVEAKRMLGRTPGTISAIRPLKDGVIADFDVTEQMLRHFIQKVHQHRFA
                     HPRVVVCVPSGVTGVEKRAVEEATLSAGARQAYLIEEPMAAAIGAGLPVAEPTGNMIV
                     DVGGGTTEVAVISLGGIVVSQSLRVGGDEMDEAIINHIKREYKLLIGQQTAEEIKLEI
                     GSAYDMREELQAEVRGRDMLTGLPKTVVLSSEEVRRALDEPVQQIIEAIKSTLDKTPP
                     ELAADIMDRGIVLAGGGALLTNFDERLRHETQMPVHLAESPLTCVAVGSGRSLEEFEA
                     IHRSAKARRNNHRRYSN"
     gene            2403..3338
                     /gene="mreC"
                     /locus_tag="VF225_03470"
     CDS             2403..3338
                     /gene="mreC"
                     /locus_tag="VF225_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7144343.1"
                     /translation="MPRNRVTAVLGSSVPRPRPQPFPSRNRSTLRRRVIVGVLVLLSL
                     VLITISFRAGSGGPVHSVEGAGATVLRPFEVAAERVARPFRDVYGYFASLVHVKRENS
                     RLKQQVNELRQKELLGQSALEQNREFRKQLKFVDSPRFPADYSPVNTRIISWATEFEQ
                     QVAIAAGSDSGIKQDTPIVTNYGLVGRVTDLTGSSAQVTLLTDENSAVPARDQTTGAI
                     GLVRHGQGENSLLLDFVRKESNVQEGDVIVTAGTRSKQYPSLFPGGIAIGVVTSVGQS
                     DTALYKQIQIDPYVDFSALDAVTALITHKRTPKAP"
     gene            3338..3853
                     /gene="mreD"
                     /locus_tag="VF225_03475"
     CDS             3338..3853
                     /gene="mreD"
                     /locus_tag="VF225_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX7144344.1"
                     /translation="MPADVAKAAGLLFLVVVVQLSVMANVDILGGHPNLLLVTLVCVS
                     LLRGAVFGAVAGFSVGLLADTGVFGTLGFTALLLTLAGYWTGRYGETTGRDRAHAPVL
                     SIAVITVLYQISALVLRYLLGEPAPASHIFAALAPTVLLNLILTVPVYALTRRLLRPQ
                     EWVTREVRLLG"
     gene            3846..5864
                     /gene="mrdA"
                     /locus_tag="VF225_03480"
     CDS             3846..5864
                     /gene="mrdA"
                     /locus_tag="VF225_03480"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7144345.1"
                     /translation="MAKRSPSRRFLPRDPHVAEPYRLTPQLALRVGILGMLALAVFAT
                     LFLRLWSLEVLNGEQLLRAAQNNQRRDIRVQAPRGPILDRNGQVLVTNVPGRAVQIWS
                     SDLPKRRGVRLREIRALARVLGLPANQIGAAIRRHRQDPLTPIRIKDNATRAQVFYLE
                     ERAEDFHGVQIVPAYLRYYPHRELAAHVLGYVSEISKEQVAALTSQGYRGGDMIGQTG
                     IESYYDRYLRGTAGLSQLRVDSLGRPISLVVPKVPAHPGNAVRLTLDAKLQKAAEDAL
                     RYGIDRAHNSNCYGCWFSNGGAIVALDPRDGSIRALASSPTYPPSLYAGRVRQKALDV
                     AGLTPRTAEAANYPALDRAINAAYPPGSTFKPVTAIAAMQEHILQPFEPLACTGSYEK
                     NGTTFKNWDPFVNEAMTLPTALARSCDTYFYQVGYRFYGMPSDRGPRLQAWASRFGFG
                     QKTGIDLGSERSGLLPTPDWRKKTYTKKTDPGHWQIDRLWKPGDSIQLAIGQKDLLVT
                     PMQMARFYAMIANGGKLVTPHLLLDVEQPSSNNSPGRAVPTSPPPAPEPTNVDAQALA
                     VVREGLFQATHATLGTSASIFGNFPVSISGKTGTAEKQIDPGDGIFRTFNQSWWCGYG
                     PSDSPDLVVCALIENGGHGGDAAAPAALKVFESFFGKQANPTGPIHSD"
     gene            5864..7042
                     /gene="rodA"
                     /locus_tag="VF225_03485"
     CDS             5864..7042
                     /gene="rodA"
                     /locus_tag="VF225_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX7144346.1"
                     /translation="MVDYVSNPRAAARARRREAAEVASFVRRLDWILIGSVAALVGYG
                     LWAIAGITHHDIAGDQGYYLTRQSVYVVIGVLGLLVATLIDPDLYRTRWRVIFGGTSF
                     LIAIVLLAGPIRGSKRWLDLGFFRFQPSEFGKVLFVLALAGFLAERSRRLHEIRTTLT
                     ALGLASIPIFLVFLQPDFGSALVYCAAVGAVLFVAGTPWTHIAVIAAGAVALAVLVLG
                     ALPAAGVPILKDYQQQRLTSFLNPSQDPGGTTYNITQSKNAIGAGQLRGRGVDNATQT
                     TLNFLPEHHTDFVFASLSEERGFVGASLLLMLYLLVVWRGLRIVTLAREPFSAIVAGG
                     LVFALLFQIFVNVGMTMGIAPITGIPLPFVSVGGSSMIANLIAIGILLSIHVRARRRR
                     "
     gene            complement(7055..7255)
                     /locus_tag="VF225_03490"
     CDS             complement(7055..7255)
                     /locus_tag="VF225_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144347.1"
                     /translation="MTGQREAHELLLIEEADAWFEYLEATRGQSVLRYKEVEPWAWAR
                     LSQRLRAIRTRRAKLKPAAEAA"
     gene            7578..>8043
                     /locus_tag="VF225_03495"
     CDS             7578..>8043
                     /locus_tag="VF225_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144348.1"
                     /translation="MRWFRSRKSEEQAPGEAAAPEAPEPLSAEPAAEAGQSSSTRPRR
                     RRGSRGGRGGSKKTATAETKTEAPEPKTATRAPKTDRKPAGQRRERERPQQRRRREPP
                     RRAPLPQAKRELLVSVDVGEQRVAVLEDDRVAEVYLERPERRSITGDIYLGTV"
BASE COUNT         1305 a         2743 c         2612 g         1383 t
ORIGIN      
        1 ggcgcaagag gagatcacct ggggcgtgtt cgaaacgccc gaagcagagc tgaacgttct
       61 cggcgaggtc agcagtctcg acatcgtgga gctgggctgt ggaacggcct acttctctgc
      121 ttggctcgcg cgtcgtggtg cccgggtcgt cggtgtcgac ccgacacctg cccagctcga
      181 aacggcgcga agactgcagc gcgagaccgg catcgagttt ccgctgatcg aggcgatcgg
      241 cgaagacgtt ccgctgccgg actcgtcgtt cgacctcgtc ctctcggagt atggagcatc
      301 cctctgggcc gatccttacc gttggatccc cgaggcggcg cgcctgttgc ggcctggcgg
      361 gcgcctgatc ttcctctgca attcgatcat ctctgcgctc tgcatgacct tggagggtgt
      421 gggcgagcag ctcgtacgac cccaattcgc gttgctcaag atcgagtggc cggacacagg
      481 cgaggtcgag ttccagctcg gtcacggcga ctggatcgac ctcatgcgcg tgaacgggtt
      541 cgagatcgag cgtctcgtcg agctgttcgc gtcgccggat agtgagacgc actcctatta
      601 caaatacgtg acgccggact gggcccggaa gtggccggcc gaagagctct gggtcgcgga
      661 gctacacgat ggctgacgcg ccgccctcgc cgccgcttct cctggcgtcg acgtctccac
      721 aacgaaaggc gatcctcgaa cagctgcacg ttccgttcga cgtcgtcgca ccgaactacg
      781 acgaagcgat accgaaaggt gcagacccaa tgcaggttgt gcgggagcat gcccgcggga
      841 aagcacggtc ggtcgcggac gtggccgagg accggccggt tgtcggtgtc gacactgccg
      901 tcgtgctcaa tggccgaatc ttcggcaagc ccgccaacgc cgccgacgcg gaggagatgc
      961 tcgaagagct gagcggcaag actcacgtcg tcgtctcggg gctctgcctg ctcacgcccg
     1021 gttgggagga cgtcgagcac gaagcgaccc aggtggcgtt ccgcgagctg cccccgcgcg
     1081 agctcggcat ctacgtcgcg acgggcgagt gggagggccg cgccggcggg tacgcgatcc
     1141 agggtcgtgg cgcggctctg gtgcgctcga tcgagggcga ctacctgaat gtggtcggac
     1201 tgcctgcggc cctgctcgtt cgtcttctcg cggagcgctt ttccggggta tacggcttcg
     1261 gttgatctag actcggtcgt ccgatgggcc tcttcgacaa tctgggcggc tttggtggcc
     1321 gcgatatggc cgtggaccta ggcacggcga acacgctcgt atacgtacgc ggccgcggga
     1381 tcgtgctctc cgagccctcc gtcgtcgcca tcgaccagcg gaccggcgag gtgcacgccg
     1441 tcggggtcga ggcaaagcgg atgctcgggc gcacgccggg gacgatttca gccattcgcc
     1501 cactcaagga cggcgtgatc gccgacttcg acgtgaccga acagatgctc cggcacttca
     1561 tccagaaggt ccaccagcat cgctttgcgc atccccgtgt cgtcgtgtgc gttccctccg
     1621 gcgtcacggg cgtcgagaag cgcgctgtcg aggaggcgac gctgagcgca ggcgctcgtc
     1681 aggcgtacct gatcgaggag ccgatggcag ccgcgatcgg cgcaggtctt cccgtggccg
     1741 aaccgacggg aaacatgatt gtcgacgtcg gcggcggcac aaccgaggtg gcagtcatct
     1801 cgcttggcgg catcgtcgtc tcgcagtcgc tgcgcgtcgg cggtgacgag atggacgagg
     1861 cgatcatcaa tcacatcaag cgcgagtaca agctgctgat cggccagcag accgccgagg
     1921 agatcaagct cgagatcggt tccgcttacg acatgcgcga agagctgcag gcggaggtcc
     1981 gcgggcgcga catgctcacg gggctgccga agacggtcgt cctcagctcc gaggaggtca
     2041 ggcgggcctt ggacgagccg gtgcagcaga tcatcgaggc gatcaagtcc actctggaca
     2101 agactcctcc ggaattggcc gccgacatca tggacagggg aatcgtcctc gccgggggcg
     2161 gggctctgct gacgaatttc gacgagcggc tccgccatga gacccagatg ccggtccacc
     2221 tggccgagag ccctctcacc tgcgtggcag tggggtccgg gcgcagtctg gaggaattcg
     2281 aggcaatcca ccggagcgcc aaggcccgcc ggaacaacca ccggcgctac tccaactagc
     2341 ttccacacaa cccccggtcc gacccaccct cgccggtttc ccagctaacg taggacattc
     2401 tcgtgcctcg caaccgcgtc accgcggtcc tgggctcgtc cgtaccgaga ccgagacccc
     2461 agccgtttcc ctcccgcaac cgctcgacgc tcaggcgccg ggtcatcgtc ggagtcctcg
     2521 tgttgctttc gctcgtactg atcacgatct cgttccgtgc cgggtcgggt ggcccggtcc
     2581 acagcgtgga gggcgccggc gcgaccgttc tgcgtccctt cgaggtggcc gccgaacgag
     2641 tcgcgcgacc gtttagggac gtctacggct acttcgcaag cctcgtgcac gtgaagcggg
     2701 agaactcgcg cctgaagcaa caggtgaacg agttgcgtca gaaagagctg ctaggccagt
     2761 cggcgctgga acagaaccga gaattccgga agcagctgaa gttcgtcgac agcccgcgct
     2821 ttccggccga ctattcgccc gtgaacacgc ggatcatctc gtgggcaacc gagttcgaac
     2881 agcaggtcgc gattgcggcg gggtccgatt ccggcatcaa gcaggacacg ccgatcgtga
     2941 cgaactacgg gctggtgggt cgcgtgaccg acctcaccgg cagttcggcg caggtgacgc
     3001 tgctgaccga cgagaacagc gccgttccgg cacgcgacca gacgacgggc gccattggcc
     3061 tcgtccggca cggtcagggt gagaactcgt tgttgctcga cttcgtgcgt aaggagtcga
     3121 acgtccagga gggcgacgtc atcgtcacgg caggcacgcg gtcgaagcaa tacccgtccc
     3181 tcttcccggg aggcatcgcg atcggcgtgg tcacgagcgt cgggcagagc gacacggcgc
     3241 tctacaagca gattcagatc gacccctacg tcgacttctc ggcgctcgac gccgtgactg
     3301 cgctgatcac acacaagcga actccgaagg cgccctgatg ccggccgacg tagcgaaagc
     3361 ggcagggctg ctgttcctgg tcgtggtcgt tcagctgtcg gtgatggcga acgtcgacat
     3421 cctcggcggt catccgaatc tgttgctcgt cacgctcgta tgcgtctcgc tcctgcgcgg
     3481 cgcggtcttc ggcgccgtcg cgggattctc ggtcggcctg ctcgccgaca caggtgtgtt
     3541 cggcacgctt ggattcactg cgctgctgtt gacgctcgcc ggctactgga cgggtcgtta
     3601 cggcgagacg acgggacgcg accgcgcgca tgccccggtg ctgtcgatcg cggtcataac
     3661 cgtcctctac cagatctccg cgctcgtgct gcgctacctg ctcggcgagc ctgcgcccgc
     3721 gagccacatc ttcgctgcgc tcgccccgac ggtgctgctc aatctgatcc tgaccgttcc
     3781 ggtctacgcg ctgacgcgcc ggctgctgcg gccccaggaa tgggtgacac gcgaggtgcg
     3841 cctccttggc taagcgcagc ccttcgcgcc gcttcctgcc gcgcgacccg cacgtggcgg
     3901 agccttaccg gctcacgcca cagcttgcct tgcgcgtcgg gatcctcggc atgctggcgc
     3961 tggcagtctt cgcgacgctc tttctccgtc tctggtcgct agaggtcctg aacggcgagc
     4021 agcttctgcg cgcagcgcag aacaatcagc ggcgagacat acgggtacag gctccgcggg
     4081 ggccgatcct cgaccgcaac ggccaagtgc tcgtgacgaa cgtccccggc cgcgccgtcc
     4141 agatctggtc gtcagacctg ccgaagcggc gcggcgtgcg gttgcgcgag atccgtgcgc
     4201 tcgcgagagt cctcggactc cccgcgaacc agatcggcgc ggcaatcaga cgccatcgcc
     4261 aggatccgct cacgcccatc aggatcaagg acaacgcgac ccgcgcacag gtcttctacc
     4321 tcgaggagcg ggccgaggac ttccacggcg tccagatcgt cccggcctat ctccgctact
     4381 acccgcatcg ggagctcgcg gcccatgtgc tcggctacgt cagcgagatc tcgaaggagc
     4441 aggtcgcagc gctgaccagt caggggtaca ggggcggcga catgatcggc cagacgggca
     4501 tcgagtcgta ctacgacagg tatctgcgtg gaaccgccgg cctctcccag ttgcgcgtcg
     4561 actcgctcgg cagaccgatc agcctggtcg tgccgaaggt tccggcgcat cccggcaacg
     4621 ccgtccgcct gaccctcgac gcgaagctgc agaaggctgc cgaggacgca ttgcgctacg
     4681 gcatcgaccg cgcccacaac tcgaactgtt acggctgctg gttctcgaac gggggcgcga
     4741 tcgtcgcgct cgacccgcgt gacggttcga ttcgcgcgct cgcgtcgtcc ccgacgtatc
     4801 cgccgtcgct ctacgccggt cgcgtgcggc agaaagcgct cgatgtcgcc ggcctcacgc
     4861 cgcgcacggc ggaagcggcc aactacccgg cgctcgaccg tgcgatcaac gccgcctatc
     4921 cgccggggtc gacgttcaag ccagtcaccg cgatcgcggc gatgcaggag cacatccttc
     4981 aacccttcga gcccctcgcc tgcaccgggt cctacgaaaa gaacggcacg accttcaaga
     5041 actgggatcc gttcgtaaac gaggcgatga ccctcccgac ggcgctcgcg aggtcctgcg
     5101 acacctactt ctaccaggtc ggctatcgct tctacggaat gccgtccgac cgcggcccgc
     5161 ggctgcaagc ctgggccagc cggttcggct tcggccagaa gaccgggatc gacctgggct
     5221 ctgagcgcag cggtctgttg ccgacgcccg actggcgcaa gaagacgtac acgaagaaga
     5281 ccgatcccgg acactggcag atcgacaggc tctggaagcc gggcgactcc attcagctcg
     5341 cgatcgggca gaaggatctg ctcgtcacgc cgatgcagat ggcgcgcttc tacgcgatga
     5401 tcgcgaacgg gggcaagctc gtgacgccac acctcctgct cgacgtcgaa cagccctcct
     5461 cgaataacag tcccggccgc gccgtgccca cgtcaccacc gcccgcgccc gagcccacga
     5521 acgtcgacgc gcaggccctc gcggtcgttc gtgagggcct gttccaggcg acacacgcca
     5581 ccctcggcac gtctgcgtcg atcttcggca attttccggt gtcgatctcc ggcaagaccg
     5641 gcactgcaga gaagcagatc gaccccggtg acggcatctt cagaaccttc aaccagtcct
     5701 ggtggtgcgg ctacggtcct tccgattcac ccgacctcgt tgtctgcgcg ctgatcgaga
     5761 acggcggcca cggcggcgac gctgccgcgc cggccgcgct gaaggtcttc gaatcgttct
     5821 tcggcaagca ggcgaacccc accggcccca ttcacagcga ctgatggtcg actacgtctc
     5881 caaccctcgt gcagcagccc gcgcccgccg ccgcgaggcg gccgaggtcg cttcgttcgt
     5941 ccggcgcctc gactggatcc tgatcggaag cgtcgcggcg ctcgtcgggt acgggctctg
     6001 ggcgatcgcc gggatcacgc accacgacat cgcgggcgac cagggctact acctgacgcg
     6061 ccagtcggtc tacgtcgtga tcggcgtgct tggtctgctc gtcgcgacgc tcatcgaccc
     6121 ggatctctac cggacgcgct ggcgtgtgat cttcggcggc acctcgttcc tgatcgcgat
     6181 cgtgctcctt gccggcccga ttcgcggttc caagcgctgg ctcgatctgg gcttcttccg
     6241 cttccagcct tccgagttcg ggaaggtcct gttcgtgctc gcgctcgccg gattcctcgc
     6301 agagcgctcg cggcgcctgc acgagattcg caccacactc actgcgctcg gtctcgcgtc
     6361 gatcccgatc ttcctcgtct tcctccagcc ggacttcggc tcagctctcg tgtactgcgc
     6421 ggccgtcgga gcggtgctct tcgtcgcggg cactccctgg acgcacatcg ccgtaattgc
     6481 agccggagca gtggcgctcg cggtcctcgt gctcggagcc ctccctgcgg ccggggtccc
     6541 gatcctcaag gactaccagc agcagcggct cacgagcttc ctcaatccct cccaggatcc
     6601 cggtggcacg acgtacaaca tcacgcagtc aaagaacgcc atcggggccg gtcagttgcg
     6661 cggccgcggc gtcgacaatg cgacgcagac cacgctgaac ttcctgccgg agcatcacac
     6721 cgacttcgtc ttcgcctcgc tctcggagga gcgaggcttc gtcggcgcct ccttgctcct
     6781 gatgctctac ctgctggtgg tctggcgcgg cttacgcatc gtgacgctgg cacgcgagcc
     6841 gttctcggcg atcgtcgcgg gcggcctcgt gttcgcgctg ctcttccaga tcttcgtgaa
     6901 cgtcggcatg acgatgggga ttgcgccgat caccggcatc ccgctgccct tcgtgagcgt
     6961 cggcggctcg tcgatgatcg cgaacctgat cgcgatcggc attctcctgt cgatccacgt
     7021 tcgtgcccgc aggcgccggt agcgggctac cagctcaggc ggcttcggcg gccggcttca
     7081 gtttcgcgcg acgcgtcctg atggcgcgca ggcgctggga gaggcgggcc caggcccaag
     7141 gctcgacctc cttgtaacgc aggacggact ggccgcgagt cgcttccagg tactcgaacc
     7201 aggcgtccgc ctcctcgatc aacagcagct cgtgcgcctc gcgttgtccg gtcattcgaa
     7261 ctccttttac gccgagaaat ccgcctcgcg cttcgcttga gccatgatga tttctcggcg
     7321 ccccgctgat ttgtcccaaa ccctaggacc tgggccggac ggcgtttcga tccgggccgt
     7381 cgtggccgcc gcgggcggag cccgaagcgg cggcgaagtg tcctgtttcc agggtggaaa
     7441 accgcccgga ggctccgggt aggccggttg gtaccctgtg ggcagatgtt ttccatcgcc
     7501 cacaacctgc cgcgcacgct ccgcgccgcc gttctagccg gcgttgctgc agcgcgcgcg
     7561 cacgaaggag atttttcttg cgctggtttc gatccagaaa gtccgaggag caggcgcccg
     7621 gcgaggctgc cgctccagaa gcacctgaac ctctaagcgc cgagcccgcc gcggaagcgg
     7681 gacaatcgag cagcaccagg ccaagacgtc gtcgcggcag tcgcggcggc cgcggcggct
     7741 cgaagaagac cgcgacggca gagacgaaga ccgaagctcc tgagccgaag acggcgacga
     7801 gggcgccgaa gaccgaccgc aagcctgccg gccaacggcg ggagcgggag cgcccgcaac
     7861 agcgccgccg ccgcgagccg ccccgccggg caccgctgcc gcaggcgaag cgcgagctcc
     7921 tcgtgtcggt cgacgtcggc gagcaacggg ttgccgtcct ggaggacgat cgggttgcgg
     7981 aggtgtatct cgagcgtccc gagcggcggt cgatcaccgg cgacatctat ctgggcacgg
     8041 tcg
//
LOCUS       DASVEU010000092         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_647444,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000092 DASVEU010000000
VERSION     DASVEU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647444"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..866
                     /locus_tag="VF225_03500"
     CDS             213..866
                     /locus_tag="VF225_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7144349.1"
                     /translation="MTSEAPDPLRVLIANERKERLKTTTGLVAAMGHHVIARSLEVSE
                     VAAATAEAMPDVALVGLGESSEHALDLISQIVQEAACPVIAILETKDAEFVNEAAKRG
                     IFAYVTDGDPEELQGAVDIVLRRFAEFHRLEGALGRRALTERAKGILMERHGLDEREA
                     FELLRSHSRRTRRKLIDVAEAVTTSHLLLATQPAPGSGNSQPSDVRTEDVPRPEPEG"
     gene            complement(987..1124)
                     /locus_tag="VF225_03505"
     CDS             complement(987..1124)
                     /locus_tag="VF225_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144350.1"
                     /translation="MLEPLAIARDRALATNDTGDARATFLHEAVRLQAEHRGLVDALE
                     L"
     gene            complement(1212..2558)
                     /locus_tag="VF225_03510"
     CDS             complement(1212..2558)
                     /locus_tag="VF225_03510"
                     /inference="COORDINATES: protein motif:HMM:NF039776.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HEX7144351.1"
                     /translation="MGTLASVRRWFMVVVGALLLSQVASGAFAADKDFGDAPDGAPYR
                     GEADIVARFPSLEASNGARHVAAGRLRLGRRVDREAGSRQVDRDRHDDGFFADLDRCR
                     VSELVFLVDARRLPAALRSAGHTAYLNAWFDWNRDGDWEDTDRCGEDVPEHRVVNVPL
                     DMASFARRPVQAIRVRARAGRQVREIWYRASLTFDELFTSPFGTGLFQHGETEDYGPR
                     PEREAEEKRKKFTAGCDPNPSFISHDRPLDVSFRYTGVPPRPSVKLVSKRPEDVAFAL
                     KPGGAGFRVRGKEDRRPAGEVAQKVTFKFRFTRGNRTIGVACEVTIYHAKVKKKRPDG
                     IPVPPIERPPTQPGIGRFRETIVLSTPCPPNATINREYKIDIPADAKNPTHRFLHETE
                     EQDLVRPFCPGSTETPEKVEIIPSPSPGQIAYRITLTIKTTVGSPERRHRVRVKFD"
BASE COUNT          506 a         1130 c         1097 g          506 t
ORIGIN      
        1 tcttgctctc gccagctgac gcgctctgcc atatccgacc tcccacgtct cagctctacc
       61 cgccggccga ggactagggc cgactcggtt tcgaccggac gctgaggtcg aggtcgccag
      121 gaccgagatg ctggggccca ggcgtctctc agcgtaccag tggggaggcc ctcgatggga
      181 ctagaatcga gtcgctagcg attgccccgc tgatgacttc cgaagcgccc gatccgcttc
      241 gcgtcctgat cgccaacgag cgaaaggaac gcctcaagac gaccaccggg ttggtggccg
      301 cgatgggtca ccacgtgatc gcgcgcagcc tcgaggtctc cgaggtcgcg gcggccacgg
      361 ccgaggcgat gcccgatgtg gcgctggtcg gcttggggga gagctccgag cacgcgctcg
      421 acctgatctc acagatcgtc caggaggccg cctgccccgt gatcgcgatc ctcgagacca
      481 aagacgccga gttcgtcaac gaagcggcca agcgggggat cttcgcgtat gtgaccgacg
      541 gcgacccgga ggaactccag ggcgcagtcg acatcgtcct gcgccgcttc gccgagttcc
      601 accgtctgga aggcgcgctc ggccgccgcg cgctcaccga gcgtgccaag gggatcctga
      661 tggagcgcca cgggctcgac gagcgggagg ccttcgagct gctgcgctca cactcccgac
      721 gaacccgccg caagctgatc gacgtggccg aggccgtcac caccagccac ctgttgctcg
      781 cgacgcagcc cgcgccgggc tccggaaact cgcagccctc cgacgtgcgc accgaggacg
      841 ttccgaggcc cgagcccgag ggctgacagg ggaccgaaat cttgaaagcg agcatgcgtt
      901 cgccccggag ctcagcgctc cgcggttcgg gctgtagggc gctcggtgct gcctcttgcg
      961 ctgactagtt cggggcgacg agcaggtcaa agctcgagtg cgtcgacgag gcccctgtgc
     1021 tcggcctgga ggcggacggc ctcgtgcagg aaggtggcgc gcgcatcgcc ggtgtcgttg
     1081 gtcgcgagcg cgcggtcgcg ggcgatcgca agcggttcga gcaccgtctc gaggacggcg
     1141 acgatgcgcg gaaggctgcc gcggccgcct cgatcagccg agacaccgca gtaagaactg
     1201 cggtcaattg gctagtcgaa tttcaccctg accctgtgcc tgcgttcggg cgagccgacc
     1261 gtcgtcttga tcgtgagcgt aatgcggtag gcgatctggc ccggcgaggg tgacgggatg
     1321 atctcgacct tctccggggt ctccgtggat cccggacaga acggccggac gagatcctgt
     1381 tcctcggtct cgtggaggaa ccggtgggtc gggttcttcg cgtcggccgg tatgtcgatc
     1441 ttgtactccc ggttgatcgt cgcgttcggt gggcagggcg tcgagagcac gatcgtctcg
     1501 cggaacctgc cgatgcccgg ctgggtgggg ggccgctcaa tcggcgggac gggaatgccg
     1561 tcggggcgct tcttcttcac cttcgcgtgg tagatcgtca cctcacacgc caccccgatc
     1621 gtcctgttcc cgcgcgtgaa cctgaacttg aaggtcacct tctgggccac ctcgccggcg
     1681 ggccgcctgt cctccttccc tctgacccgg aagccggcgc cgcccggctt gagggcgaac
     1741 gcgacgtcct cggggcgctt ggagacgagc ttgacgctcg gcctcggcgg caccccggtg
     1801 taccggaagg agacgtcgag cggccggtcg tggcttatga agctcgggtt cgggtcgcag
     1861 ccggcggtga acttcttcct cttctcctcc gcctcccgct ccgggcgggg gccgtagtcc
     1921 tcggtctcac cgtgttggaa gagccccgtg ccgaagggtg aggtgaacag ctcgtcgaac
     1981 gtgaggctcg ccctgtacca gatctcgcgc acctgcctgc cggcgcgcgc cctgaccctg
     2041 atcgcctgaa cgggcctgcg cgcgaacgac gccatgtcca agggcacgtt cactacccgg
     2101 tgctcgggca cgtcctcccc acaccgatca gtgtcctccc agtcgccgtc gcggttccag
     2161 tcgaaccagg cgttgaggta ggcggtatgc cccgcagaac gcagcgcggc cggcagccgg
     2221 cgcgcgtcaa ccaggaacac cagctcgctc acgcggcaac ggtcgaggtc cgcgaagaag
     2281 ccgtcgtcgt ggcggtcgcg gtccacctgc ctcgaaccgg cctcgcggtc gacgcgccgt
     2341 ccgagcctga gccggcctgc agccacgtgg cgcgctccgt tcgaggcctc gagcgagggg
     2401 aagcgggcga cgatgtcagc ttccccgcgg tagggggccc cgtcgggagc gtcgccgaag
     2461 tccttgtcgg cggcaaacgc tcccgaagcg acctgggaca ggagcagcgc cccgactacg
     2521 accatgaacc accgtctcac gctggcgagc gttcccatcg gcctctcctt ctctcgacca
     2581 ccggttcctt acgcgccacg ggccggcgcg gatcagcgcc gcgggagcag cactcatgac
     2641 acggagcgga cccgcgctgc atgcctggga aaagcgttcc gaacaggtcc ccgtcgctac
     2701 tgcagcgctc cgggaagggc gtaccccgcg gcggccagtt cgggccgtcg gacgcaatcc
     2761 ctccggccga gcagtacacg agcgcgccgc agtcgagctc ccccgtaatc ggttcgcgcg
     2821 cggccgatgc ggcgctgcac gcgaccaggc gcgtaagcca gctggtacag ccaagaccgg
     2881 cagcggttgt ccgtcgcatg cgagtccctc cacaacgact ctacacccgc catctacagc
     2941 tcgcgaacgt accgccgagc taccggaaga ccgcggcaaa gtcgggttcg aacgccgagc
     3001 tgggaaacgc gatccccacc agcggaaatt gagcgccaca acatgccgtc gaacacgccc
     3061 gcctaggacg acccagaggt gggcgacacg acggacaacg cgcgcctgct cctgtcagcg
     3121 gttgcggttg tcctcgcgcg ccgggcgacc gtactcgcgg gtcgtcccgt gcgggcgtct
     3181 ccccggacga ccgtgagacg ctcgctcgtc gcgccgaagc gggagagccg ctctgctgg
//
LOCUS       DASVEU010000093         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_840892,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000093 DASVEU010000000
VERSION     DASVEU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840892"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF225_03515"
     CDS             <1..743
                     /locus_tag="VF225_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144352.1"
                     /translation="RNGYGFDDLVRRSSESRPVKTMFGGDENDTTVGLALEEARERGA
                     RRNKGPGKRVDDGAVVVLGSGNLALIYLMEERRRLTLEEIETRHPRLVPALRSHPHVG
                     FVLAHSATDGPVALGPHGARYLQDERVEGDDPLRAFSLTAARHLLRADGFHNAPDLYA
                     NSFYDPQVDEGCAFEELISFHGGLGGSQTRPFVLSPVHLPIPEEPLIGAVPVHHLLKG
                     WRESLQRANRQPGPARFQRPVEPRAVQR"
     gene            779..2104
                     /locus_tag="VF225_03520"
     CDS             779..2104
                     /locus_tag="VF225_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7144353.1"
                     /translation="MAAVGALIVAVSLIWLALSMAANDPIGLGISFVAVFFVAFSAWL
                     VLTRRGVLRLLFLPLGLLALVALATYTLDQIVMLIVLAGLLALFGVGARYAVRNHPTS
                     LHTAHRRTRLAQPACRGVLIINPRSGGGKAERFNLVEEAKRRRIEPLLLAPGDDLCEL
                     ARGAVDRGADVIGMAGGDGSQALVATVAMEHDLAHVCVPAGTRNHFALDLGLDRNDVI
                     GALDAFSEGVERRIDLASLNERIFVNNASLGVYAHVVQSDAYRDAKLGTWKRMLPEML
                     GPDAAEIDLRFDAPSAKDWANASLVIVSNNPYQLRRLRGAGTRPRLDSGHLGIFATRI
                     QSAAAVAKLVTLGTVGQHRRFGGVLEWSSTKFEIRASEPVAVGLDGEAVVLSPPLVFV
                     SLPGALRVRVPRHASGVSPAGGAVTLTRRNVGTLLRIAAGKPPVAPPHR"
     gene            2286..2696
                     /locus_tag="VF225_03525"
     CDS             2286..2696
                     /locus_tag="VF225_03525"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144354.1"
                     /translation="MTTGSSLEARERANVRVGSILIRCVRFDELVAFWQAALGYVPRE
                     PPQDGWVVLTDPSGTGPNISLDRVAEDRLPPLGDLSRVHLDLYTADQDAEVERLLGLG
                     ATRFEREYRPDEDFVVLVDPDGNRFCVIDKRDES"
     gene            complement(2820..3098)
                     /locus_tag="VF225_03530"
     CDS             complement(2820..3098)
                     /locus_tag="VF225_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144355.1"
                     /translation="MATARFVKTGITPDEYDQMREKLGVGDTPPAGGVFHAAAVGEDG
                     KIRVFEVWDSRQQAEAWGEKVMAVRTEAGFGDGPPSIEYLDVHKVVQR"
     gene            complement(3218..4051)
                     /locus_tag="VF225_03535"
     CDS             complement(3218..4051)
                     /locus_tag="VF225_03535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144356.1"
                     /translation="MSPELDERGEWVLMATVQEYLDAYDRQLRTDAETPSAVAVTRLG
                     PLRLVTFASGRGFVTYGDLGGADGAVIARWVEGALSHYREDPVITRVKWKTRAHDRAP
                     ALHDALVRNGFVPEEPESIMIGEAALLAVEVPVPTGIHLRRVTSEPDVRALSEMQEDV
                     FGGGFAQEMADALLRRLGFGDGMELWVAEADGEIVSSGRLEPVPGTEFAGIWGGATRP
                     EWRGRGLYRALTAARARSALVLGKRLIHSDSTEASRPILERAGLVKVSTTTPYLWVAG
                     G"
     gene            4189..4842
                     /locus_tag="VF225_03540"
     CDS             4189..4842
                     /locus_tag="VF225_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144357.1"
                     /translation="MAQIDGIDEISDGRCPWRPVRFHFGITSFGVNAFTAHQVGARLI
                     NEHDESQEHDLQEELYLVQRGRATFELDGERVDAPVGTLVFVPPAVKRTAFAEEPGTT
                     LVAIGGTPGKAYEDKGGDIWMPLNHLYESGQYAEAADQGRELIEAHPECAGPLYNLAC
                     CESLAGRTDDAIKHLRLAIDRHEPFRSLAAEDGDFDPIRDEAAFKELVQGNAAKTTG"
BASE COUNT          814 a         1771 c         1598 g          804 t
ORIGIN      
        1 agcgcaacgg gtacgggttc gacgacctcg ttcgccgctc gagcgagtct cggcccgtaa
       61 agaccatgtt cggaggcgac gagaacgaca cgaccgtcgg gctcgctctc gaggaagcgc
      121 gcgagcgagg cgctcggaga aacaagggtc ctgggaagcg cgtcgacgac ggcgccgtcg
      181 tcgtgctcgg ctccggcaac ctcgcactca tctacctcat ggaggagcgg cgccgcctca
      241 cgctcgagga gatcgagacc cgccacccac gactcgtgcc cgcccttcgc agtcacccac
      301 acgtgggctt cgtgctggct cactccgcga ccgacgggcc cgtggcactc gggccacacg
      361 gggcgcggta tctgcaggac gagcgcgtcg aaggcgacga ccctctccgg gcgttctccc
      421 tcacggccgc ccgccacctc ctacgggcag acggcttcca caacgcgccg gatctctacg
      481 cgaacagctt ctacgacccc caggtcgacg aaggctgcgc cttcgaagag ctgatctcgt
      541 tccacggcgg cttgggaggc tcacagacgc gccccttcgt cctctcgccc gtccacctgc
      601 cgatccccga ggagcccctg atcggagcgg tgcctgtcca ccacctgctc aaagggtggc
      661 gtgaatctct gcagagggca aatcgtcaac ccggaccggc gcgctttcag cggccggtcg
      721 aaccccgcgc ggtacaacgg tgatcgaacc aggcatcagc ccgcatcgcc gagcgcaaat
      781 ggccgcagtc ggtgcactga tcgtcgccgt gtctttgatc tggctcgcgc tctcgatggc
      841 ggcgaacgac ccaatcgggc tgggcatctc gttcgtcgcc gtcttcttcg tcgccttctc
      901 cgcgtggctc gtgttgacaa gacgcggcgt tctccgactg ctcttcctgc ctcttggcct
      961 cctcgccctc gtcgcgttgg ccacctacac cttggaccag atagtcatgc tgatcgtctt
     1021 ggccggcctg ctcgcgctct ttggtgttgg ggcgcgctat gcggtgcgca atcaccccac
     1081 cagcctccac accgcccacc ggcgtacccg gctcgcgcag ccagcgtgcc gaggggtgct
     1141 gatcattaac ccacgttccg gcggggggaa ggccgagcga ttcaacctcg tcgaggaggc
     1201 gaagcgacga cgcatcgagc cgttgctgct cgcgccggga gacgacctct gcgaactggc
     1261 taggggggcg gtcgaccggg gcgccgacgt catcgggatg gccggcgggg acggatcgca
     1321 ggcgctggtg gcaacggtcg cgatggagca cgaccttgcc cacgtctgcg tgcctgccgg
     1381 gacccggaat catttcgcac tcgatctcgg gctcgaccga aacgacgtca tcggcgcact
     1441 cgatgctttc agcgaaggcg tcgagcgccg tatcgatctt gcgtcgttga acgagcgcat
     1501 cttcgtgaac aacgcctcgc tcggggtcta tgcacacgtc gtgcagtcgg acgcctaccg
     1561 cgacgcgaag ctgggaacct ggaagcggat gctccccgag atgctggggc ccgacgccgc
     1621 cgagatcgac ttgcggttcg acgcgccgag cgccaaggac tgggcaaacg catcgctcgt
     1681 gatcgtctcg aacaacccgt accagctccg gcggctccgg ggggccggca cgcgcccgcg
     1741 tctcgacagc ggacacctcg gcatcttcgc gacgcggatc cagagcgccg ccgcggtcgc
     1801 gaagctcgtg accctcggca cggtcggtca gcaccggcga ttcggcggcg tgctcgagtg
     1861 gtcgtcgacc aagttcgaaa tccgggcaag cgaaccggtt gcggtcggcc tcgacggcga
     1921 agcggtcgtc ctctcgcccc cgctcgtctt cgtctccttg ccaggagcgc tgcgcgtccg
     1981 cgtgccgcgc cacgcaagcg gcgtctcgcc ggcaggagga gcggtcacgc tcacccggcg
     2041 caacgtcggg acgctacttc gaattgctgc cgggaagccg ccagtcgcgc ccccacatcg
     2101 gtgaggacgc acgcagcagc cgtcgagcgc ggcggccagc cgcgctcaac cgaacgaccg
     2161 ccttgacgca gcacctcggc gacgcggacg gtctgcgcgg gaagtgggcg gaggtgaact
     2221 tgacgagcca gcagcagatc gttggggcgc tgctgacacg cgtgacgagt gggtaggttc
     2281 ggtatgtgac cacaggttca tcgttggagg ctcgcgagcg ggcgaacgtc agggtcggct
     2341 cgatcctcat tcgctgtgtg cggttcgacg agctggtcgc cttctggcaa gcagcgctcg
     2401 gttacgtccc gcgcgagccg ccgcaagacg gctgggtcgt gctcaccgac ccgagcggga
     2461 caggtccaaa catctcgctc gaccgggtcg ccgaggaccg tctgccgcca cttggcgacc
     2521 tcagccgagt tcatctagac ctctacaccg ccgatcagga cgcggaggtc gagcggcttc
     2581 tcggccttgg tgcgacccgc ttcgagcgcg agtatcgacc ggacgaagac ttcgtcgtcc
     2641 tcgttgaccc cgacggcaac cgcttctgcg tgatcgacaa acgcgacgag tcgtagcgcg
     2701 agtgcggcgg agcgcgctgc tccagcggcc gcttggctca cgggagattt ccgaacgctg
     2761 agcagacgag gtcggcgcga gcgaccaggg ccgcccgcgc cacccttgtc ccgaaccgac
     2821 tagcgctgaa ccaccttgtg gacgtcgagg tattcgatcg agggcggccc atctccgaag
     2881 cccgcctccg tgcgcaccgc catcaccttc tcgccccagg cctcagcctg ttgacgcgag
     2941 tcccatacct cgaagactcg gatcttcccg tcttcgccga ccgcggccgc gtggaacacg
     3001 cccccagccg gtggggtgtc accaacaccg agcttctcgc gcatctggtc gtactcgtcg
     3061 ggtgtgatcc ccgtcttgac gaaccttgct gtcgccacgc caacctcctc gctagacgcc
     3121 tcgacattac tccggcgccg cgcgtcaggc tcgccctgct cagggtcatt cccgcacact
     3181 ggcgcatgcg cggcagcagg cgcgtcgccc gacccgccta cccgccggcg acccacaggt
     3241 agggcgtcgt cgtcgagacc ttgacgagtc ccgctcgttc gaggatcgga cgcgaggctt
     3301 cggtcgagtc gctgtggatc agcctcttgc caagcacgag cgctgatcgc gcacgggccg
     3361 ccgtgagcgc tcggtaaagc ccgcgcccac gccattccgg tcgtgtggcg ccgccccaga
     3421 tcccggcgaa ctctgtacca ggtaccggct ccaggcggcc cgagctgacg atctctccgt
     3481 cggcctctgc cacccagagt tccatcccat ccccgaagcc cagccttcgg agcagcgcat
     3541 ctgccatctc ctgggcgaac ccgcctccga agacgtcttc ctgcatctca ctgagcgcgc
     3601 ggacgtccgg ctcgctcgtg acccggcgga ggtggatccc cgtcggcact gggacctcaa
     3661 ccgccaggag agcggcttcg ccgatcatga tcgactccgg ctcctcgggc acgaacccgt
     3721 ttcgtacgag cgcgtcgtga agcgccggcg cgcgatcgtg cgcgcgcgtc ttccacttga
     3781 cgcgggttat cacaggatcc tcacggtagt gcgaaagagc gccctccacc caccgagcga
     3841 tcaccgcgcc atccgcgcct ccgagatccc catacgtgac gaaaccccga ccgcttgcga
     3901 aggtcacgag cctcagcggg ccgaggcgcg tcacggccac cgcactcggc gtttcggcgt
     3961 ccgtacgaag ctgccggtcg tacgcgtcga gatactcctg cacagttgcc atcagcaccc
     4021 attctccacg ctcgtccagc tcgggtgaca ttcccgcacg ctggcccagg cgcgcgtcta
     4081 cggtgtcgcc gcggcgaggt ggtcgatcct tgccggggac gctcgttagg ttgagtcgcc
     4141 tgcgtatccc atcacccgaa ggaggacagc cgaatgagcg gttacgcggt ggcccagatt
     4201 gacgggatcg acgagatcag tgacggccgc tgtccttggc ggcccgtccg gttccacttc
     4261 ggcatcacgt ccttcggcgt caatgcgttc acagctcatc aggtgggcgc tcggctcatc
     4321 aacgagcacg acgagtcaca ggagcatgac ttgcaggagg agctctacct cgtccagcgc
     4381 gggcgcgcaa cgttcgagct cgacggcgag cgggtggacg cacccgtggg cacgctcgtc
     4441 ttcgttcccc ccgccgtgaa gcggacggcg ttcgcagagg agccggggac gacgctcgtc
     4501 gcgatcggcg gaactccggg gaaggcgtac gaggacaagg gcggcgacat ctggatgccc
     4561 ctcaaccatc tctacgagtc gggccagtac gccgaggccg ccgatcaggg gcgcgagttg
     4621 atcgaagctc atcctgagtg cgcagggccg ctctacaacc tcgcgtgctg cgagagcctc
     4681 gccggccgga cagacgacgc gatcaagcac ctccgactcg cgatcgaccg ccacgaaccg
     4741 ttccgttcgc tagctgccga ggacggcgac ttcgacccga tccgcgacga ggccgcgttc
     4801 aaggagctcg ttcagggcaa cgcagccaaa accacggggt gacagacccg caacggatcg
     4861 cttcagtccc aaacgccggc cgagccgagc gtacgctcgc ttggatgctc cgcgctggga
     4921 tgaacgcgcg gatctcgtgt gagcagcacg ccgcgcacgg ccaagaaagg gcgctcgatg
     4981 acacgcc
//
LOCUS       DASVEU010000094         7595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1229801,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000094 DASVEU010000000
VERSION     DASVEU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229801"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF225_03545"
     CDS             <1..805
                     /locus_tag="VF225_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7144358.1"
                     /translation="VHDLRSREYGSAGGMASDLMTPIVFWAVGRAVRLALARARAARA
                     DAEQASAHSADLARQAVDDERRHIARELHDIVTHSLGVVVLQAQGARRVLDQREPTVT
                     EALGTIEHSGRAALEEMRRLLGLFRDQGERSGLAPQPRLADTTELVRHITNAGLPTVL
                     AVEGKSVKLEPGVELSAYRVVQEALTNSLKHARGSRAEVRIRYRPLELELEITDVGGR
                     RNGDPVGGAGRGLLGMRERVTFYGGTLEHGPHPEGGFRVHATFPLRSPR"
     gene            802..1446
                     /locus_tag="VF225_03550"
     CDS             802..1446
                     /locus_tag="VF225_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144359.1"
                     /translation="MSIRVLIADDEAMIRTGLRLVLDREPDINVVDEAEDGRSTVERT
                     AELEPDVVLMDVRMPKMDGIEATRRIVGSQVRSRVIVLTTFDEDEYVYDALAAGASGF
                     LLKYAPADDLVTAIRVVAAGDAMLAPTATRRLLERFIDRSEAPEAVKVAALTAREQQV
                     LKLIAEGMSNTEIADELVLSRATVKTHVANILEKLQARDRVQAAAIAYKSRLMS"
     gene            complement(1645..2982)
                     /locus_tag="VF225_03555"
     CDS             complement(1645..2982)
                     /locus_tag="VF225_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144360.1"
                     /translation="MAEDSVRQPTLAEAKVEMDLLKQAGFNAVRVTQIWAPGETTPSV
                     ADQRILRNVVQAARLDRMQVLLTVMNFGNRTTPLTEQARGEFAAYAAALASALPQVRY
                     FVIGNEPNINRYWLPQFSQDGTDAAAPAYEALLAQAYDALKAVSPKIVVLGGALSPHG
                     GDDPDSIRPTHSPTAFLTDLGAAYRASSRTRPIMDGLALHPYEDNSSVAPIKGVHPNS
                     TTIALADYDKLVALLGTVFAGTAQQGSTLPIYYDEFGVEAQIPPAKASLYTGREPVTV
                     KPVPEAVQGVYYRQAVELAFCQPNVRGLFLFHAIDERDLNRWQSGLYYVDRSAKSDLR
                     PVRAAVSQAHRGVVTHCPGLALRPRGSLVVAVRPLPRATLLCDMDCTYVVTVRRGSRV
                     ANRSRGRAVGGIATRIRLGLLHTGLHYTVTALLSAPVNPGHARVLRAVPFTVP"
     gene            complement(3184..4740)
                     /locus_tag="VF225_03560"
     CDS             complement(3184..4740)
                     /locus_tag="VF225_03560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7144361.1"
                     /translation="MVAVVLLGLAAAVWAWSGARLAADARALARVEVQPFGGTLARAR
                     AFRPDGRTIPLLVRGGRVTPRVQLMPGESVSVEVVIRRPGWLAWALGSKHVERLTLRA
                     PVAQVSERWLTVSAGTPVRVGFDQSVSAVAAGLGGDLAFRRLDSARRSFAFSRRAAAG
                     SIEVAAAARSWERLGAPVAVSWFPPARLPVVVSSPLLGAPLAPAAPIRLTFSKPLAAV
                     LGSALPRLSPPTRGHWHQTDSHTLVFIPSGFGAGFATDLRVQLPRAVALIRPGGGRLR
                     TTRRLEWTVPPGSLLRLEQLLAQAGYLPLDWQPSGPPVARTPPAELEAAVEAPKGRFS
                     WRYLNSPSELRAIWRPTRPNEITRGAVMTFEDSHGLAVDGFAGAAVWHALLADAIAGK
                     RRTSGYSYVYVRSSIPQSLILWHNGHTVLSSPGNTGVPAAPTQLGTFPVFEHIAVGTM
                     SGTNPDGSHYHDPGIRYISYFNGGDAIHAFNRASFGTPQSLGCVELPLASAAKVWPYT
                     PIGTLVTIEN"
     gene            5100..6206
                     /locus_tag="VF225_03565"
     CDS             5100..6206
                     /locus_tag="VF225_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX7144362.1"
                     /translation="MLDARTDVVGSLLRPPDLLAAQEKLANGELTPSAFKRVEDKAVD
                     DALELQEEAGLAVVTDGEMRRLSFQSQLPAAVHGFGEWDLDAFLWGEWQSDELGELRV
                     ERPQLAVEQTLRRKRSLATEEFVYARGRTDRVVKVTLPSPSLFANFWDPKRSKAAYRN
                     LEEFLADVAELLREEVDELVRIGAVYIQLDAPHYPLLVDPTYRQFYESRGWPAERWLE
                     LGVELDNHVIGDHPGVTFGFHLCRGNQASRWLVEGGYDWLAERLFPRVRAQRLLLEYD
                     DARSGTFAPLRAVPDEKIVVLGLVTTKTSRRETVEELVVRIREAAKVVPLDRLALSPQ
                     CGFATSVIGNALSSDDQRAKLRTIVDAAAVVWPP"
     gene            6203..7117
                     /locus_tag="VF225_03570"
     CDS             6203..7117
                     /locus_tag="VF225_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7144363.1"
                     /translation="MTAPVAVIEDRSVRRRGQLFVALAALAWSSAGVLQRELSVGTTT
                     QLAGRALFACIALAGFVAVSNRGRTIAAFRSMGTAGFAVAICTAIASGSFIVALNHAT
                     VANVLFLQAAAPIAAALLAWIALGEAITRRAFVAMGIAVLGVALMVGGPGSGGVLGVG
                     VSLLMTLAFAATIVITRHRRDVSMAPAICLSQALVVVAVAPFAHPSTVTGHDLAFLVL
                     LGAGQVGLGLAFLTVGARLIPAAEVALITLLEIVLGPLWVWIVLGERPALIAVIGGVV
                     VIFAVVLQMTQSVQTQASHSPVRAVLPD"
     gene            7127..>7595
                     /locus_tag="VF225_03575"
     CDS             7127..>7595
                     /locus_tag="VF225_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX7144364.1"
                     /translation="MTVVLTGNDLSLDEVVAVARGSEPVELAPEAVVRMRESRAIVER
                     ALDRGDVVYGFTTGVASRKRVRIEPADVEEFNRRLIQSHRVGQGAEAPADVVRATMLR
                     MANGFATGTTGSRPDLAERLVAALNGKETPQVHLLGSVGQSDLAPNADIAHHLF"
BASE COUNT         1242 a         2623 c         2561 g         1169 t
ORIGIN      
        1 tgtccacgac cttcggagcc gtgaatacgg aagcgccggc ggtatggcga gcgatctgat
       61 gaccccgatc gtgttctggg cggttgggcg agccgtccgt ctcgcgctcg cacgcgcgcg
      121 agcagcgaga gcggacgccg agcaggccag cgcgcacagc gccgacctcg cccgccaagc
      181 ggtcgacgac gagcgccgcc acatcgcgcg cgagctgcac gacatcgtca cccacagcct
      241 cggcgtcgtc gtcttgcaag cgcagggtgc gcggcgcgtg ctcgatcagc gggagccgac
      301 cgtcaccgag gcgctcggca ccatcgagca ctcgggtcgc gctgcgctcg aggagatgcg
      361 ccgcctcctg gggttgttcc gcgaccaggg agagcggtcg gggctcgccc cccagccacg
      421 tctcgccgat acgaccgagc tcgtacgcca catcaccaat gcaggccttc cgactgtcct
      481 cgctgtcgag ggcaaatcgg tcaagctcga gcccggcgtg gagctctccg cctatcgcgt
      541 cgtccaggag gcgcttacga acagcctcaa gcacgcccgt ggcagccgcg ccgaagtgcg
      601 catccgctac cggccactcg agctcgaact cgaaatcaca gacgtaggcg gccgacgcaa
      661 cggcgacccg gtcggcggcg ccggccgcgg cctgctcggg atgcgcgaac gtgtgacctt
      721 ctacggcggc acactcgaac acggtccaca cccagagggc ggcttccgcg ttcacgccac
      781 ctttccgctc cggtcgccca gatgagcatc cgcgtcctca tcgccgacga cgaggcgatg
      841 atccgtaccg gtctgcggct tgtcctcgac cgcgagcccg acatcaacgt cgtcgacgag
      901 gcagaggacg gacggtcgac cgtggagcgc acagccgagc tcgaaccgga cgtcgtcctc
      961 atggacgtcc gcatgccgaa gatggacggg atcgaagcga cgcgccgcat cgtcggctcg
     1021 caggtccggt cgcgagtgat cgtattgacg acgttcgacg aagacgagta cgtctacgac
     1081 gccctcgccg ccggcgccag cggcttcctc ctcaagtacg cgccggccga cgacctcgtc
     1141 accgcgatcc gcgtcgtcgc cgcaggcgac gcgatgctcg cccccaccgc gacacgccgc
     1201 ctgctcgaac gcttcatcga ccgaagcgaa gctccggagg ccgtcaaggt cgccgcgcta
     1261 accgcccgcg agcagcaggt cctgaaactg attgcggaag gcatgtcaaa caccgagatc
     1321 gccgacgagc tcgtactcag ccgcgccacg gtcaagaccc acgtcgccaa catcctcgaa
     1381 aaactccagg ctcgcgaccg cgtccaagcc gccgccatcg cctacaagtc ccgactcatg
     1441 tcgtaggaag gtgctcgtca cgccctcgcg tgggcacagg ttcatggtgg tgatcagccg
     1501 catccaccct ctccgctatc tgagaaacaa ctaactcagc aaaggtatga gaaggcgacc
     1561 cgtgcattga tgtcgcactt ccgccaaagt gcgacacgtt gtaggcgcgc gcgagaggga
     1621 gtagtcgacg gccgctgccg tacgtcaggg gacggtaaag ggaacggcgc ggaggactct
     1681 cgcgtggccg gggttgacgg gcgcgctgag caacgcggtg accgtgtagt ggaggccggt
     1741 gtgcagcaac ccgagccgga tccgtgtcgc tatgccgcca acggcgcggc cgcgcgagcg
     1801 attcgctacg cggctgccgc ggcgaacggt gacgacgtac gtgcagtcca tgtcgcagag
     1861 aagcgtcgcc cgcggcagtg gacgaactgc gacgacgagg ctgccgcgcg gccggagcgc
     1921 gagccctggg cagtgggtta cgacgccgcg atgggcctgc gagaccgccg cgcgcaccgg
     1981 gcgaagatcc gacttcgccg accggtcgac gtagtacaga cccgactgcc agcggttgag
     2041 atcgcgttcg tcgatcgcat ggaagaggaa caggccgcgc acgttcggct ggcagaaggc
     2101 gagctcgacc gcctggcggt aatacacgcc ctgcactgct tctggcaccg gcttcaccgt
     2161 caccggctcc cgtcccgtgt agaggctcgc cttcgccggc gggatctgcg cctcgacacc
     2221 gaactcgtcg tagtagatcg gcagtgtcga cccctgctgc gccgtcccgg cgaagacggt
     2281 gccaagcagc gcgaccagct tgtcgtagtc ggcgagcgcg atcgtggtcg agttcggatg
     2341 gacgcctttg atcggcgcga cgctcgagtt gtcttcatag gggtggagcg cgagcccgtc
     2401 catgatcggc ctcgtccggc tgctcgcgcg gtaggccgcg ccgaggtcgg tcaggaatgc
     2461 ggtcggcgag tgcgtcgggc ggatcgagtc ggggtcgtcg ccgccgtgcg gtgagagcgc
     2521 gccgccgagg acgacgatct tcggcgagac cgccttcagc gcgtcgtagg cctgcgcgag
     2581 caatgcctca taggccggtg cggctgcatc ggttccgtcc tggctgaact gcggcaacca
     2641 gtagcggttg atgttcggct cgttgccgat cacgaaatac cgcacctgcg gcagcgcgct
     2701 cgccaacgcc gccgcatacg ccgcgaactc cccacgcgcc tgctctgtca gtggcgtcgt
     2761 tcggttgccg aagttcatga ccgtcaacag gacctgcatc ctgtccagcc gcgcggcctg
     2821 cacgacgttg cggaggatcc tctgatcggc cacgctgggc gtcgtctcac ccggcgccca
     2881 gatctgcgtc acccgaacag cgttgaaccc cgcttgcttg aggagatcca tctccacctt
     2941 cgcctcggcg agcgtcggct gccgcacgct gtcctcagcc accccgatca gcatcgccgg
     3001 accgcctgca cgtgcgtacg gtgccggaag aacacacccc aggagcacac aggcgacggc
     3061 gaaacgcttc ataaccccgc gcccacgata atgccagccg cctcggccgc atcgaaatcc
     3121 tccctcggat gtcgcgctcc tggcggaaac gcgacaagaa cctcagtccg ctctgtggct
     3181 cgctcagttc tcgatggtga cgagcgtgcc gatcggggtg tagggccaga ccttggcggc
     3241 ggaggcgagc ggcagctcga cgcagccgag gctctgcggc gtgccgaagg aggcgcggtt
     3301 gaaggcgtgg atggcatcgc cgccgttgaa gtagctgatg tagcgaatgc ccggatcgtg
     3361 gtagtgggag ccgtccggat tcgttccgct catcgtgccg accgcaatgt gctcgaacac
     3421 ggggaaagtg ccgagctggg tcggggcggc gggcacgccg gtgttgccgg gcgagctgag
     3481 gacggtgtgc ccgttgtgcc agaggatcag tgactgggga atgctgctgc gcacgtagac
     3541 gtagctgtag ccgctggtgc ggcgtttacc ggcgatcgcg tcagcgagga gcgcgtgcca
     3601 gaccgccgct cccgcgaagc cgtcgaccgc gaggccgtgg ctgtcctcga acgtcatcac
     3661 ggccccgcgc gtgatctcgt tcggccgagt ggggcgccag atcgcccgta actcactcgg
     3721 gctgttgagg tagcgccagc tgaagcggcc cttcggcgcc tcgacggcgg cttccagttc
     3781 ggcaggcggg gtgcgtgcaa ccggtggacc cgacggctgc cagtcaagcg gtaggtagcc
     3841 cgcttgggcg agcaactgct ccagacgcaa cagcgagccg ggcggcaccg tccattcgag
     3901 ccggcgggtc gtgcgcaggc gtccgccccc aggtctaatt agcgccaccg cgcgcggcag
     3961 ctggacccgc agatcggtag cgaagccggc cccgaaaccc gaaggaatga agacaagcgt
     4021 gtggctgtcc gtctgatgcc agtggccgcg tgtaggcggc gacagcctcg gcagcgcgga
     4081 gccgagcacc gctgcgagcg gcttggaaaa cgtgagccgg atcggggcgg cgggcgcgag
     4141 cggcgcgccc aacaacggac tgctgaccac caccggcagg cgcgcgggcg ggaaccagct
     4201 caccgcaacc ggggcgccga gccgctccca cgatcgcgca gcggcggcca cctcgatcga
     4261 gcccgcagcg gcccggcggc tgaatgcgaa cgagcggcgt gcactatcca accgccggaa
     4321 cgcgaggtcg ccgcccagtc ccgcggcgac cgcgctcacc gactggtcga accccacccg
     4381 caccggcgtt cccgcagaaa ccgtcagcca acgctcgctc acctgcgcga ccggggcgcg
     4441 cagcgtcaat cgttcgacgt gcttgctccc gagtgcccac gccagccacc cgggtcgacg
     4501 aatcaccacc tcgaccgaca cgctctcacc cggcatcaac tgcacccgtg gcgtcacgcg
     4561 cccaccacgt accaaaagcg ggatcgtgcg cccgtccggt cggaacgccc gcgcgcgcgc
     4621 aagcgtcccc ccgaacggct gcacctccac ccgcgccagc gccctcgcgt cagctgccag
     4681 cctcgccccc gaccacgccc acacagccgc ggccaacccg agcagcacga ccgccaccat
     4741 gcccacagcc cacggcaagc gccggcgcgc gcgccgaggc gaacccaaac cgcgcgacaa
     4801 aaggggcgag ggccccgacc caacggacac ccgttgagga tacgtacgcc cagagcagcg
     4861 tccccgcatg gatcagctcg ccgccgacag ccgcaccgcg cgacgggatc ccctcacgag
     4921 gagttcctga gccggcggag cggtgcgaac catccgatca gttacgtcac cgctcgacgg
     4981 tcgggtccac cgcacgagca acgcgcaagc aggagaccgt atccgtcgga agcggggggt
     5041 gccgagctgg aaaatgggag tgttgagccc gccgaccgga cgtccgtaga gttaggtccg
     5101 tgctcgacgc gcgaacggac gtcgtcggca gtcttcttcg cccgccagat ttgctcgcag
     5161 cgcaggagaa gctcgccaac ggggagttga cgccctctgc gttcaagcgg gtggaggaca
     5221 aggcggtcga cgatgccctc gagctccagg aggaggcagg cctcgcggtc gtcaccgacg
     5281 gcgagatgcg ccgactctcg tttcagagcc agctccctgc tgccgtccac ggcttcggcg
     5341 agtgggacct cgacgcgttc ctctggggcg agtggcaaag cgacgagctc ggtgagcttc
     5401 gcgtcgaacg accgcagctc gcggtggagc agacgcttcg gcgcaaacgc tcgctcgcga
     5461 cagaagagtt cgtgtacgcg cgcggccgca ccgaccgggt cgtcaaggtg acgttaccca
     5521 gccccagcct gttcgcgaac ttctgggacc cgaaacgctc gaaggccgca taccggaacc
     5581 tcgaggagtt cctcgccgac gtcgccgagc tccttcgtga ggaggtcgat gagctcgtgc
     5641 ggatcggcgc ggtctacatc cagttggacg caccgcacta cccgctgctc gtcgatccga
     5701 cctaccggca gttctacgaa agccgcggct ggccggccga gcggtggctc gagctcggcg
     5761 tcgagctcga caaccacgtc atcggagatc acccgggcgt caccttcggc ttccacctct
     5821 gccgcggtaa ccaggcgagc cgctggctcg tcgaaggggg ttacgactgg ctcgccgagc
     5881 ggctgtttcc ccgcgttcgc gcacagcggc ttttgctcga gtacgacgac gcgaggtccg
     5941 ggacgttcgc tccgctgcgc gctgttcccg acgagaagat cgtcgtgctc ggactcgtga
     6001 cgaccaagac gtcgcgccgc gagacggtcg aggaactcgt cgtccgaatt cgggaggctg
     6061 cgaaggtcgt tccgctcgat cggctggcgc tctccccgca atgcggcttc gccacgtccg
     6121 tcatcggcaa cgccttgagc agcgacgacc agcgcgcgaa gctgcgtacg atcgtcgacg
     6181 cggctgcggt cgtctggccg ccgtgacggc accggtcgcc gtgatcgagg ataggagcgt
     6241 ccgccgtcga gggcagctgt tcgttgctct cgcggccttg gcgtggtcga gcgccggtgt
     6301 gctgcagcgg gagctttcgg tcggcaccac cacacagctc gccgggcgcg cgttgttcgc
     6361 gtgcatcgcc ctggccggct tcgtcgcggt ttcgaatcgt ggccgcacga tcgcggcgtt
     6421 tcgttcgatg ggaaccgcgg gtttcgccgt tgcaatctgt acggcgatcg cgtcaggctc
     6481 gttcatcgtc gccctcaacc acgcgacggt tgcgaacgtc ctcttccttc aggccgcggc
     6541 tcccattgct gccgcgctgc tcgcgtggat cgcgctgggc gaagcgatca cacgccgcgc
     6601 gttcgttgcg atgggcatcg cggtgctcgg cgtcgcattg atggtcggcg ggcccggtag
     6661 cggcggcgtg cttggcgttg gagtttcgct cttgatgacg ctcgcttttg cggcgaccat
     6721 cgtcatcacc cggcatcgac gtgacgtgtc catggcgccg gcgatttgtc tctcgcaggc
     6781 gcttgtcgtc gtggcggtcg ccccattcgc ccatcccagc accgtgaccg ggcacgacct
     6841 ggcattcctc gtgctgctcg gcgcggggca ggtgggtctc gggctcgctt ttctgaccgt
     6901 cggtgcccgc ttgattccgg ctgcagaggt cgcgctgatc acactgctgg agatcgtcct
     6961 cgggccgctc tgggtgtgga tcgtgctcgg ggaacgaccg gcgctgatcg ccgtcatcgg
     7021 cggcgttgtc gtcatcttcg cagtggtgct gcagatgacg cagagcgtgc aaacgcaggc
     7081 cagccacagc ccggttcgcg ccgtactacc cgactagagt gcgcgcgtga ccgtcgtcct
     7141 caccggcaac gatctcagct tggacgaggt tgtcgcggtt gcgcgcggca gcgagccggt
     7201 cgaactcgcg ccggaagcgg tcgtgaggat gcgcgagtca cgtgcaatcg tcgagcgcgc
     7261 gcttgaccgc ggtgatgtcg tgtacggctt cacgaccggt gtcgcctcgc gcaagcgcgt
     7321 gcggatcgag ccggctgacg tcgaggagtt caaccgacgg ctgatccaga gccaccgcgt
     7381 cggccagggg gcggaggcgc cggcagacgt cgtcagggcg acgatgctgc ggatggcgaa
     7441 cgggttcgcc acggggacaa ccggcagcag gcccgatctc gccgagcgtc tggtcgccgc
     7501 gctgaacggg aaggagacgc cgcaagttca tctgcttgga tccgtgggcc agagcgatct
     7561 tgctccgaac gccgacatcg cacatcatct cttcc
//
LOCUS       DASVEU010000095        14729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_455077,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000095 DASVEU010000000
VERSION     DASVEU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455077"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /gene="ligD"
                     /locus_tag="VF225_03580"
     CDS             <1..956
                     /gene="ligD"
                     /locus_tag="VF225_03580"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX7144365.1"
                     /translation="SPRERRTSADNSPPLLAEAKTVKLSSADRVLYPEDGITKGDVFE
                     YYKQVGPTIVPHLKDRPFTMKRYPHGITGEVFFQKQAPKHLPSWIRTRQFRTWPREGG
                     SRLVDFALVNSPEAVLFMVQNNCIDMNAWYSRVDKPDRPDFVLFDLDPPDDGFELAIE
                     VAQLIRELLEELELPGYVKTSGADGIHVVAPITRRSSFKQTYDFAERASRLLEQRHPG
                     KVTTEWLKKKREGVLVDHRQNGHGKTIASVYSVRPKPGAPVSTPLRWEELKPGIRPRD
                     FGMEEALRRIEEHGDLFVPVLEDPRPLAPAAKKLEKLGSDG"
     gene            953..2029
                     /locus_tag="VF225_03585"
     CDS             953..2029
                     /locus_tag="VF225_03585"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX7144366.1"
                     /translation="MSEPNRDLIMKALERVIDPELRKPVTELDMVRAVEIDGGDVRVT
                     IALTIAGCPLRDSFEQQVAEALRDVPGVERVALGFDVMTPDERQALTTKLRGGLHERS
                     KGLSLDASTRVLAVCSGKGGVGKSTLTANLAVALSQAGKQVGVLDADVYGHSIPHVLG
                     IRQKPVLVDKMIVPPVKHDLKLMSIGFFLDDNAPVMWRGPMLHRALEQFLSDVHWGAL
                     DVLVVDMPPGTGDVAISLGQLLPRAETIVVTTPQPAAQEVASRAGVMAQKTGMRLVGV
                     VENMTGDIFGSGGGERLAAELDIPLLGCVSLDPLLRECGDAGEPLMATAPDSQSAREL
                     RQISDQLLGLDRGTIVKPLTLVAP"
     gene            complement(2026..2535)
                     /locus_tag="VF225_03590"
     CDS             complement(2026..2535)
                     /locus_tag="VF225_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144367.1"
                     /translation="MVPEAKLENGVPQGEGWFVVNARDSRWLHNELGAYCSFEGKGEA
                     AFEQLGINLNVVPPGMPMAMYHEEPGQEAFLVLRGECLLVVEGQERPLKAWDFVHCPP
                     RTKHVIVGAGEAPALVLAVGARKGPASYPVDETAVRLGVGVDKEVASPAEAYARFSQL
                     EEGPAPDLS"
     gene            2557..3285
                     /locus_tag="VF225_03595"
     CDS             2557..3285
                     /locus_tag="VF225_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX7144368.1"
                     /translation="MQVEKRIRALGFSEEDSRTLADHFLDAERRGKRGHGLARVEWLE
                     TLTGLDPSAEPECVVAEACYERWEGRGALGYLTLAAICAGQLEDPPEAARLIVAHDCF
                     PTGMLGYWARRVATGGLLAVLTATSPPRLGHPDGGPKLTGTNPLAIAIPSSDDRPIVS
                     DVSMGRVTYGDVLAGAASEEELVPFGGEHAYKAFALALGLQLLVDSLSGDGYGAVMLV
                     AQPAADPVPALRARAAGSRLPGDA"
     gene            3282..3929
                     /locus_tag="VF225_03600"
     CDS             3282..3929
                     /locus_tag="VF225_03600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7144369.1"
                     /translation="MIHALPPAIVAGELVRLQTRQRVVALTFDGGGNADAAKSVLAIL
                     KRERVPGTFFLTGHFVQTYPLLSRAIGRRYSVANHTVDHLDLRRLSTPAATSEITRAG
                     TMIRRATGRDPRPLFRFPYGARDARTLGIVRRLGYASVRWTVDTWGWMGLRLQSVAGA
                     QRRVLDHLVPGEIVLMHLGAARDRSTIDSRALPGVIRAVRARGYRFVTLQGIRAP"
     gene            complement(3941..4792)
                     /locus_tag="VF225_03605"
     CDS             complement(3941..4792)
                     /locus_tag="VF225_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX7144370.1"
                     /translation="MARSDVVATYSIVACDLEADQWGVAVQSKFLAVGSVVPWAEPRV
                     GAIATQSYANPRYGPDGLALLRKGRSAEEVVELLTAADDGRAERQVGVVDAEGGAATF
                     TGEACHDWAGGRTGNGYAAQGNILVSHETVDALAVTFEQNGHLSLAERLVECLAAAQA
                     AGGDRRGQQSASLLIVEKDAGYANLSDVVVDLRIDDHELPIVELRRLHGLHNEVFGIT
                     PPEDWLTIDEALASELRDSLAKLGYDGELERAFNDWAGTENLEERVDGVERIDPVVLA
                     ALRKQSG"
     gene            complement(4782..5060)
                     /locus_tag="VF225_03610"
     CDS             complement(4782..5060)
                     /locus_tag="VF225_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144371.1"
                     /translation="MRERWFGATGRRVPEIAVEGELDLGDALVLDDVADERPLHEAHE
                     AGRPIVVRAASAEAVKAALAHPEVASALVPAERRDLLDLDLRELTYGP"
     gene            complement(5057..5749)
                     /locus_tag="VF225_03615"
     CDS             complement(5057..5749)
                     /locus_tag="VF225_03615"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7144372.1"
                     /translation="MAVASLTWLGHASFRLDSPSGKRVYVDPWLDNPKCPESEKDPER
                     CDVIAVTHGHSDHLGSVVAITQKLGSMPIVAMIELKDWLKGQGAQTDDLPGPNKGGTV
                     DVDGVKFTLTNAFHSSSADDGTYLGEAAGIVIELEDGKKIYFAGDTCVFGDMRLLGRI
                     YSPDVAVLPIGGHFTMDPREAAVACELLGVKRVVPSHYGTFPLLAGSPDELKRLTSVE
                     VHAIDPGESIEL"
     gene            5824..6174
                     /locus_tag="VF225_03620"
     CDS             5824..6174
                     /locus_tag="VF225_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144373.1"
                     /translation="MARRLLPAMLVLAALLSDAGGAHGLALAFLLFAIPAAFTLALDC
                     YGDLLESRCSGVRPALAGLSLFLLVLSAALRSPAVIGGLPKLAVSALVITLVLYAAIS
                     VGALLPGRALPESA"
     gene            6254..8389
                     /locus_tag="VF225_03625"
     CDS             6254..8389
                     /locus_tag="VF225_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7144374.1"
                     /translation="MLARLAHLVHRRRGRFIALWVVLTAFGVFATMRVADRWFESFSI
                     PGYSAYETNQKTLKTFGTGEQAPMVAVFRSKGDVTKQDLAPAIEKAAAVNPGSRVSSY
                     FDSHNDIFVSRDRHTTFAEIYGPGQNTFSTNPHIKDVRKAVEAATPPGVEAHLTGLLP
                     LYDATSGGGTGPSVLTEALIGGVGALVILMFVFGTLPAVAIPLAIAVTSILNTFTLVW
                     ILTYVTDVSIIVQFLIALVGLGVAIDYALLMIFRFREELASGEDVETALEQTMTHAGR
                     SVIVSGSTVAVGLLSMVLLPLPFIRSIGIGGMLIPAVSVIVSITLLPALLSLLGHRIN
                     KLRVMPRRFVTVTSPESGWWGKWAGLVIRRPVPVAAVGAVIVGVLLALGSQLNPSEAQ
                     AKDFPGKGDAITGRQVLADAGISPGVMKPFVVLTPADETNQVVQRLGGVEGVVGVTAP
                     ESWRKDGLALVEAFPSVDGSSKAVRGTISRVKDALPAGASLGGVAPEDRDFVHAVYGN
                     FPYVLLFVVLLTYVLLARAFRSLLLPLKAVILNLVSLGAAYGIIVFIFQQGHGSEAIW
                     GVPATQSIIPWIPLMIFAFLFGLSMDYEVFMLTRMREAYDETGSTREAVRLGLARTGK
                     LVTSAALVLMFAFFVLSTGPGTDIKQFGIGLAAGIIFDATVIRALLVPAIMMLMGRWN
                     WWLPAWAARPLRTQPSEPVLEAQPAPEAA"
     gene            complement(8386..9852)
                     /locus_tag="VF225_03630"
     CDS             complement(8386..9852)
                     /locus_tag="VF225_03630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7144375.1"
                     /translation="MIPRPHIDWFALAPVNALLAASALALLCAVLAPARSRKAVTATL
                     CTLGYATALGFAIALYVKSAHGHGVVVDAFRRDRLAELTAIIVAGSGILATAVSYRER
                     WREDHIGEYYALLAAAGGGMVFFVGANNLLTLFLGLEWFSICLYILCAIDRDRLSGLE
                     AALKYLVIGGFGSAVLLFGAALVYGATGALDFPGIAAAGHAHDALLLFGLAMIIAGLA
                     FKASAAPFHMWTPDVYEGAPTPLTAFMAAATKTVALVVTLRILVTAFPGDAYVWTRAL
                     AVIAVCSLVIGNLAALVQKGVKRMLAYSSISHAGFMLIAVASGSSLGARALLYYLVPY
                     AAMSIGSFAVVAARERELGVPVTFANMAGFGWERPLLGISMWTFMLGFAGFPLTGGFV
                     GKFYVFAAAYRHGWTWLVIVGVLATLVSLYYYLGLVRAMFMRRTEDLQLAPIGGSPPR
                     EALLQSAVLACLVVTVGSFFAVQPLIDVAKHAASSLPF"
     gene            complement(9849..11288)
                     /locus_tag="VF225_03635"
     CDS             complement(9849..11288)
                     /locus_tag="VF225_03635"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7144376.1"
                     /translation="MTTALIVLPLAAALVIWVLPLNDVSAGALALLAALAEVGVWIQL
                     VARYDFAQGGLQFAQQRNWFSDLHVSYHVGVYGFSVWLIGLTVVAMAAAIAYGFWAGR
                     DRMRAYMGLMLFLTGAVIGVFTAQDLLLFYAFFEAMMIPLYVLVGVWGGPGRLGATLK
                     FVLFTMAGSLLMLASIVVYGLSQNTFDLVQIGQSTSDWVFLGFVAAFAIKAPLFPLHG
                     WLPDAYRESSPEVAAILSGVVSKAAAYGFLRIAITKFPEPAQHYRAPILALAAIGLIY
                     GSLLAFRAPDIRGVIAYSSLAQMGLITMGVFSVNDLGLDGSVLQMVNHGLISTTLFLL
                     AGMVERRTLTGELSRLGGMARGRPVLATVLLTTGVITLAVPLSSAFAGEFLILAGVFR
                     HGWGWSVVGAVAMVLAAAYALRLVSAILHRDVGPAVSDAALDLRPAELGVVLPMLACL
                     LALSAWPAAITQHSFRGDHGQADVAQGFK"
     gene            complement(11289..13166)
                     /gene="nuoL"
                     /locus_tag="VF225_03640"
     CDS             complement(11289..13166)
                     /gene="nuoL"
                     /locus_tag="VF225_03640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7144377.1"
                     /translation="MISGAWICLLSPLAAAGLITLAGERISRRGAGYLATLSCFVAFG
                     GAIASFVGLLGRDGGHRVVVSTAWTWISGGSYRSGFEVMIDPLSVFMMLIVAGVGALI
                     VAYSIGYMDGDDEERRYFAYMALFVFSMLLLVQGGNLLLLLAGWGMVGLSSYLLIGFW
                     HERPSAIAAAKKAFIMNAIGDATMALALFLLIKKAGSVEFGILGGQYSSTVATLVALG
                     LLGGAVAKSAQLPLQTWLPDAMEGPTPVSALIHAATMVTAGVYLIVRTHSIFEAAPHV
                     ADLAAGLGAATLLLAGLIALVQVDIKRVIAYSTMSQIGYMFLGVGVGAYANGMFHLMT
                     HAFFKALLFMAAGIVIHALADEQDIRKMGGLRQLMPRTYLAFLIGSVALVGIFPFAGF
                     FSKDSILAAAMDHGAYGYVLWAAGLAGTFLTGLYTFRLLFLVFWGEPSPFVREHFHAL
                     KRDVVGVSLAVPVAILAVLSVVGGWIQWEPLWHPIETWLQTVAEPTVSPKSWQEAVSS
                     ALALLLGLGGIFLAWLIYGARRVAVPRFAFAQRVLEHKFYFDELYDALFYRPTVWAAK
                     GLYRWVEDPLVDESSNSLGQDTRDLGRLVSRAQTGLLRTYALAIASSVAVLALVFVAV
                     R"
     gene            complement(13163..13468)
                     /gene="nuoK"
                     /locus_tag="VF225_03645"
     CDS             complement(13163..13468)
                     /gene="nuoK"
                     /locus_tag="VF225_03645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7144378.1"
                     /translation="MGPGTAWYLGVAGFLFGVGALGVLIRRNPLIVLLSLEIMLNGGN
                     LALIAFARNLNNGDGQIFALAVMAVAASEVVVGLGLIVALNRKRIPLDVDRLSSLRG"
     gene            complement(13468..14025)
                     /locus_tag="VF225_03650"
     CDS             complement(13468..14025)
                     /locus_tag="VF225_03650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7144379.1"
                     /translation="MIVASVGNVLVWAVWLGAAFACLASAVAVVSFTNPFYSALALIG
                     NLGSLAVLYLLLSSEFVAAAQVLVYAGAVMVMFLFVIAYVGPRTDSPWTGGPSWQTIG
                     AVLAAGALLVEIVVVIGLKAGGALAHSHHVGAAFGTPSYIGRLFLTDHLLAFEITSIV
                     LLVAAIGGVILGAHSRTTAEEVPPL"
     gene            complement(14022..14519)
                     /gene="nuoI"
                     /locus_tag="VF225_03655"
     CDS             complement(14022..14519)
                     /gene="nuoI"
                     /locus_tag="VF225_03655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX7144380.1"
                     /translation="MSPQPLDSVKGFAVTFRQLFKKPITQQYPEYKRPVYPRFRGRHR
                     LHLHENGLEKCVGCSLCAAACPADCIRVVADENTPENRVSPGERYARIYEINMSRCIF
                     CGYCELACPFDAITLGNEYEISEYSRDDLIYTKDMLLAGPIKKIPVADRGLYDTPIPY
                     YKDNS"
     gene            complement(14516..>14729)
                     /locus_tag="VF225_03660"
     CDS             complement(14516..>14729)
                     /locus_tag="VF225_03660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7144381.1"
                     /translation="FFGGWHGPYAPLGPLWFLLKVFIFISVFIWIRASVPRLRYDQLM
                     RFGWKILLPVATINAVITALLVVWVS"
BASE COUNT         2564 a         4898 c         5028 g         2239 t
ORIGIN      
        1 cgtcgccgcg ggaacgccgg acctctgccg acaactcacc gccgctcttg gctgaagcga
       61 agaccgtcaa gctgagctcc gccgaccgcg tcctctatcc ggaggatggg atcacgaagg
      121 gcgacgtctt cgagtactac aaacaggtcg gccccacgat cgtgccgcac ctgaaagacc
      181 gcccgttcac gatgaagcgc tacccgcacg ggatcacggg cgaggtcttc ttccagaagc
      241 aagcgcctaa gcacctgccg agctggatca ggacgcgcca gttccgcacc tggccacgcg
      301 aaggaggttc gcggctcgtc gactttgcac tcgtaaattc gcccgaagcc gtgctcttca
      361 tggtgcagaa caactgcatc gacatgaacg cgtggtactc acgtgtcgac aagcccgatc
      421 ggccggactt cgtcctcttc gatctcgatc caccggacga cggcttcgag ctcgccatcg
      481 aggtcgccca gctcatccgc gagctgctcg aggagctcga gctgccgggt tacgtgaaga
      541 cgagcggcgc ggacgggatc catgtcgtcg cgccgatcac acgccgctcc tcgttcaagc
      601 agacgtacga cttcgccgag agggcttcgc gcctgctcga gcagcgacat cccggcaaag
      661 tcacgacgga gtggctgaag aagaaacgcg agggtgtgct cgtcgaccat cgccagaacg
      721 ggcacgggaa gacgatcgcg tcggtgtact cggttcgacc caagcctggc gcgccggtat
      781 cgacgccact gcgctgggag gagctgaagc ccggcatccg gccgcgggac ttcggcatgg
      841 aggaggcgct gcgccgcatc gaagagcacg gtgacctctt cgtacccgtg ctcgaggacc
      901 cacgacccct cgcgcccgcg gcgaagaaat tggaaaagct aggctcagac ggatgagcga
      961 gccgaatcgc gacctgatca tgaaggcgct cgaacgcgtc atcgatccgg agctgcgcaa
     1021 gccggtgacg gagctcgaca tggtgcgcgc cgtcgagatc gacggcggcg acgtgcgcgt
     1081 cacgatcgcg ctgaccattg ctggttgccc gttgcgggat tcgttcgagc aacaggtcgc
     1141 cgaggcgctg cgagacgtgc cgggcgtcga gcgtgttgcc ctcggcttcg acgtgatgac
     1201 gcccgacgag cgccaggcgc tgacgaccaa gctgcgcggc ggcctccacg agcgttcgaa
     1261 gggcctctcg ctcgacgcgt cgacacgcgt gctcgccgtg tgcagcggga aaggcggagt
     1321 cggcaagtcc actctgacgg cgaacctcgc cgtcgcgctt tcgcaggccg gcaagcaggt
     1381 gggcgtgctc gacgcagatg tctacggcca ctcgatcccg cacgtgctcg gcatccgcca
     1441 gaagccggtg ctcgtcgaca agatgatcgt ccctccggtc aaacacgatc tgaagctgat
     1501 gtcgatcggt ttcttcctcg acgacaacgc gccggtgatg tggcgcggcc ccatgttgca
     1561 tcgtgcgctc gagcagtttc tgtcggacgt gcactgggga gccctcgacg tgctggtcgt
     1621 cgacatgccg ccggggacgg gtgatgttgc gatctcgctg ggacagttgc tgccgcgggc
     1681 cgaaacgatc gtcgtcacga cgccgcagcc ggctgcgcag gaggttgcct cacgggcggg
     1741 cgtgatggcg caaaagacgg gcatgcggct cgtcggcgtc gtcgagaaca tgaccggtga
     1801 catcttcggg tcgggcggcg gcgaacgtct ggccgctgag ctcgacatcc cgctcctcgg
     1861 ctgcgtctcg ctcgatccgc tcctgcgtga atgcggagac gcgggcgagc cactgatggc
     1921 gaccgcgccc gactcgcaga gcgcacgcga gctgcggcag atctccgatc agttgctcgg
     1981 gctcgaccgc ggcacgatcg tcaagccgct gacactcgtc gcgccctagg aaaggtcggg
     2041 cgcgggccct tcctcgagct gcgagaagcg cgcgtatgcc tccgccggcg acgccacctc
     2101 tttgtcgacg ccgacgccca ggcgaaccgc cgtctcgtcg acgggatagc tcgcaggccc
     2161 tttgcgggct ccgacggcaa ggacgagcgc gggagcttca ccggcaccga cgatgacgtg
     2221 cttcgtccgc ggcgggcaat gaacgaaatc ccaggctttg agcggacgct cctgaccctc
     2281 cacgacgagc aggcactcac cgcgcaagac gaggaaagcc tcctgccccg gctcctcgtg
     2341 gtacatcgcc atcggcattc ccggaggcac cacgttcagg ttgatcccga gctgctcgaa
     2401 ggcggcctca cccttgccct cgaagctgca ataggcaccg agctcgttgt gcagccaccg
     2461 ggaatcgcgg gcattgacga cgaaccagcc ctcgccctgc ggaacgccgt tctcgagctt
     2521 cgcctccggg accatctcat aagtatcctc gccgcggtgc aggtcgagaa gcggatccgt
     2581 gcgctcggtt tctcggaaga ggactcgcgc acgcttgccg accactttct ggatgccgag
     2641 cggcgcggca agcgcggcca cggtctcgca cgtgtcgagt ggctcgagac gctgacaggc
     2701 ctcgatccgt ccgcggagcc cgagtgcgta gtcgccgagg cctgctacga gcgctgggag
     2761 ggccgcggcg cgctcgggta tctgacgctt gcggcgatct gcgcaggcca actcgaggat
     2821 ccgcccgagg ccgcgcgcct gatcgtcgcg cacgactgct tcccgaccgg aatgctcggc
     2881 tactgggcac gccgcgtcgc caccggaggc ctcctggccg tgctgacggc cacgtcaccg
     2941 ccgcggctcg gtcatccgga tggaggcccg aagctcaccg ggacgaatcc tctggccatc
     3001 gcgatcccga gctcggacga caggccgatc gtcagcgacg tctcaatggg acgtgtgacc
     3061 tacggggacg tgctcgccgg agctgcctcg gaagaggagc tggtgccgtt cggcggtgag
     3121 catgcgtaca aggccttcgc gctggcgctt gggctccaac tgctcgtcga ttcgctcagc
     3181 ggcgacggct atggcgcggt catgctcgtc gcgcagcctg ctgccgatcc cgttcccgcc
     3241 ctgcgggccc gggcagccgg gtcgcgcctc ccgggcgacg cgtgattcac gcgctgccgc
     3301 ccgcgatcgt ggcgggcgag ctcgtccggc tgcagacccg acagcgggtc gtcgccctga
     3361 cgttcgacgg tggcggcaac gcagacgcgg ccaaaagcgt gctcgcgatc ctcaagcgcg
     3421 agcgcgtgcc gggcacgttc ttcctcaccg gacacttcgt ccagacatat ccgctgctgt
     3481 cgcgcgcgat cggacgtcgg tactcggtcg cgaatcacac ggtcgaccat ctcgacctgc
     3541 ggcgtctgtc gaccccggcg gcaacaagcg agatcacacg ggcggggacg atgatccgcc
     3601 gcgcgaccgg gcgtgatcca cgaccactgt tccgctttcc gtacggcgca cgagacgcgc
     3661 ggacactcgg catcgtgcgg cgcctcggct acgcgtcggt tcgctggaca gtggacacct
     3721 ggggctggat ggggctgagg ttgcagtcgg tagcaggtgc gcaacgccgt gtcctcgacc
     3781 acctcgtgcc gggtgagatc gtcctgatgc atctcggcgc ggcccgcgat cgcagcacga
     3841 tcgactcccg cgcgctcccg ggggtgatcc gggcggtgcg cgcgcggggc tatcggttcg
     3901 tgacgctcca aggcattcgc gcgccttagg atctcggcgc ctaacccgac tgcttgcgca
     3961 gggcggcgag tacgaccgga tcgattcgct cgaccccgtc gacgcgctcc tcgaggttct
     4021 ccgttccggc ccagtcgttg aaggctcgtt ccagctcgcc gtcgtagccg agcttggcga
     4081 ggctgtcgcg gagctccgag gccagcgctt cgtcgatggt gagccagtcc tcgggcggcg
     4141 tgatcccgaa gacctcgttg tgcaatccgt gcagccgccg caattcgacg atcgggagct
     4201 cgtgatcgtc gatgcgcaga tcgacgacga cgtccgaaag gttcgcgtag ccggcgtcct
     4261 tctccacgat cagaagcgag gccgactgct ggccgcgccg gtcgccgccg gcggcctgcg
     4321 cagcggcgag gcactcgacc aggcgctccg cgagtgagag gtgtccgttc tgctcgaacg
     4381 tcaccgcaag cgcgtcgacc gtctcgtgcg acacgaggat gttcccctgg gccgcgtagc
     4441 cgttccccgt tcttccgccg gcccagtcgt ggcacgcctc gccggtgaac gtggccgcgc
     4501 ctccttccgc gtcgacgacg cccacctgcc tctcggcccg gccgtcgtcg gcggcggtca
     4561 gcaattcgac cacctcttcg gccgagcgtc ccttgcgcag tagggcgagc ccgtcggggc
     4621 cgtagcgtgg gttcgcgtag gactgcgtcg cgattgcgcc tacgcgcggc tcggcccagg
     4681 ggactacgga gccgacggcg aggaacttcg attgcacggc cacgccccac tgatccgctt
     4741 cgagatcgca cgcgacgatc gaataggtcg cgacgacgtc gctacgggcc atacgtgagc
     4801 tcgcggaggt ccaggtcgag caggtcgcgg cgctcggccg gaacgagcgc cgacgcaacc
     4861 tccggatgcg cgagcgctgc cttcactgcc tccgccgaag cagctcgcac gacgatcggc
     4921 cgtccggcct cgtgcgcttc gtgcagtggg cgctcatcgg ccacgtcgtc gagcacgagc
     4981 gcgtcgccga ggtcgagctc gccctcgacg gcgatctccg ggacgcggcg tcccgtcgca
     5041 ccgaaccagc gctctctcac agctcgatcg actcgcctgg atcgatcgca tgcacctcga
     5101 cgctcgtcag ccgcttcagc tcgtcgggcg atccagccag gagtgggaac gtcccgtagt
     5161 gcgaggggac gacgcgtttc acgccgagga gctcacaggc cactgcggcc tcccggggat
     5221 ccatcgtgaa gtgaccgccg atcggcagca cggcgacgtc cggcgaatag atgcgaccga
     5281 ggagccgcat gtcgccgaag acgcaggtgt cgcctgcgaa atagatcttc ttgccgtctt
     5341 cgagctcgat gacgatcccg gctgcctcgc ccaggtaggt cccgtcgtcc gccgagctcg
     5401 agtggaacgc attcgtcagc gtgaacttga caccgtcgac atcgacggtc ccgcccttgt
     5461 tcggcccggg gaggtcgtcc gtctgcgcgc cctgcccctt cagccagtcc ttcagctcga
     5521 tcatcgcgac gatcggcatc gagccgagct tttgggtaat tgcgacgacc gatccgaggt
     5581 ggtccgaatg gccgtgcgtg accgcgatca cgtcgcagcg ctccgggtcc ttttccgact
     5641 cgggacactt cgggttgtcg agccacggat cgacgtagac gcgcttgccg ctgggggaat
     5701 ccaggcggaa tgaagcgtgt cccaaccaag tcagcgacgc tacggccatc gatctcctct
     5761 cgtttgcagg tggcggcgcg gagcgtaaag gaacgtccga gttgagggcg aagcttgtcg
     5821 aggatggctc gccgcctgct tcccgccatg ctcgtgctgg cggccctgct ttccgacgcc
     5881 ggcggcgccc acggtctcgc gctcgcgttc ctgctcttcg cgattccggc ggcatttacg
     5941 ctcgcgctgg attgctacgg agaccttctg gagtcgcgct gcagcggcgt ccggcccgct
     6001 ctggcaggac tgtccctgtt cctcctcgtg ctcagcgcgg cgctgcggag cccggccgtg
     6061 atcgggggcc tcccgaagct ggccgtgtcg gcgctcgtga tcacgctcgt gctctatgcc
     6121 gcgatctcgg tcggcgcgct gttgcccggc cgggccctgc ccgagtcggc ctgacgcagc
     6181 aaggccgctg gtgcggataa ccgcaccacc ggacacctgc ccaccgcccc ggctcgacag
     6241 gtaaacatca ggcgtgctcg cacgcttggc ccacctcgtg caccgccgcc gcggccgctt
     6301 catcgccctc tgggtggtgc tgaccgcatt cggcgtcttc gcgacgatgc gcgtcgccga
     6361 tcgctggttc gagtcgttct cgattccggg ctactcggcg tacgagacga accagaagac
     6421 gttgaagacg ttcggtaccg gcgagcaggc cccgatggtg gctgtcttcc ggtccaaggg
     6481 cgacgtcacg aagcaagatc tggcgccggc gatcgagaag gccgcggcgg tgaacccggg
     6541 ctcgcgggtc agctcgtact tcgattcgca caacgacatc ttcgtctcgc gcgaccgcca
     6601 cacgacgttc gcagagatct acggaccggg tcagaacacc ttctcgacca accctcacat
     6661 caaggacgtg cgcaaggctg tggaggcggc caccccgccg ggcgtggagg cccatctcac
     6721 cggcctcctc ccgctctacg acgcgacgag cgggggcggg accggtccga gcgtgctgac
     6781 cgaggcactg atcggcggcg tcggagcgct cgtcatcctc atgttcgtgt tcgggacgct
     6841 gcccgcggtc gcaatcccgc tcgccatcgc ggtgacctcg atcctcaaca cgttcaccct
     6901 cgtgtggatc ctgacctacg tcactgacgt ctcgatcatc gtccagttcc tgatcgcgct
     6961 cgtcggtctc ggggtcgcca tcgactacgc gctgctgatg atcttccgct tccgcgagga
     7021 gctggcctcc ggcgaggacg tcgagactgc cctggaacag acgatgacgc atgcaggccg
     7081 gtcggtgatc gtctcaggct cgactgtcgc tgtcggactg ctgagcatgg tcttgctccc
     7141 gctgccgttc atcaggtcga tcggaatcgg cggcatgctg atcccggctg tgtcggtgat
     7201 cgtctcgatc acgctcctcc ctgcgctcct ctcgttgctc gggcatcgca tcaacaagtt
     7261 gcgcgtgatg ccgaggcgct tcgtcaccgt cacctccccg gaatcgggct ggtggggcaa
     7321 gtgggcgggt ctcgtcatcc gcaggccggt gccggtggcc gccgtcgggg ccgtgatcgt
     7381 cggcgtgctc ctcgcgctcg gctcgcagct caatccgagc gaggctcagg ccaaggactt
     7441 ccccggcaaa ggcgatgcga tcaccggccg acaggtgctg gccgacgcag gcatctcccc
     7501 tggagtgatg aagccgttcg tcgtcttgac gcctgcggac gaaacgaatc aggtcgtgca
     7561 acggctgggc ggggtcgagg gcgtcgtcgg cgtcacggcc ccggagagct ggcgcaaaga
     7621 cggtctcgcc cttgtcgagg cttttccgag tgtcgacggc tcgagcaagg cggtacgagg
     7681 gacgatctcg cgggtgaagg acgctttgcc tgccggtgca agtctcggcg gcgtcgcgcc
     7741 ggaggaccgc gacttcgtgc atgcggtgta cggcaacttc ccgtatgtcc tcctgttcgt
     7801 cgtcctgctg acctacgtcc tgctcgcgcg cgcgttccgt tcgctgctgc taccgctgaa
     7861 ggccgtgatc ctcaatctgg tgtcgctcgg agcggcctac gggatcatcg tcttcatctt
     7921 ccagcagggc cacggctccg aagcgatctg gggcgtgccc gcgacacagt cgatcatccc
     7981 gtggatcccg ctgatgatct tcgcgttcct gttcgggctc tcgatggact acgaggtctt
     8041 catgttgacg cgcatgcgtg aggcttacga cgagacgggg agcacccgcg aggcggtcag
     8101 actcggactg gcgcgcacgg ggaagctcgt caccagcgcg gcgctcgtcc tgatgttcgc
     8161 cttcttcgtg ctctcgacgg gcccgggcac cgacatcaag cagttcggga tcgggctggc
     8221 ggccgggatc atcttcgatg cgaccgtgat ccgggccctg cttgtcccgg cgatcatgat
     8281 gctgatgggc cgttggaact ggtggctgcc ggcctgggcg gcccgcccgc tccgcacgca
     8341 gccgagcgag ccggtgctcg aagcacagcc cgctccggaa gccgcctaga acggcagcga
     8401 ggaggcggcg tgctttgcca cgtcgatcag cggctggacg gcgaagaacg agccgaccgt
     8461 gacgacgagg caggcgagga ctgcgctttg cagcagcgcc tcgcgcgggg gtgagccacc
     8521 tatcggtgcg agctgcagat cttccgtgcg acgcatgaac atcgcgcgga cgagaccgag
     8581 gtagtagtag aggctgacga gcgtcgcgag cacgccgacg atcacgagcc acgtccagcc
     8641 gtgtcggtac gcagcagcga agacgtagaa cttgccgacg aacccgccgg tgagcggaaa
     8701 gccggcgaag ccgagcatga acgtccacat cgagatcccg agcaagggcc gctcccagcc
     8761 gaacccggcc atgttcgcga aggtcacagg cacaccgagc tcgcgctccc gcgccgcgac
     8821 gactgcgaac gagccgatcg acatcgccgc atacggaacg aggtagtaga gcagcgcccg
     8881 agcgccgagg ctcgagcccg aagcgacggc gatcagcatg aagccggcat gcgagatgga
     8941 cgagtaggcg agcattcgct tcacgccctt ctgcacgagc gcggcgagat tgccgatcac
     9001 cagcgagcag acggcaatca ctgcgagcgc gcgcgtccag acataggcgt cgccggggaa
     9061 cgcggtgacg aggatgcgca gcgtgacgac gagggcgacg gtcttcgttg cggccgccat
     9121 gaacgccgtc agcggggtcg gcgcgccttc gtagacatcg ggcgtccaca tatggaacgg
     9181 cgccgcggag gccttgaacg ccaggccggc gatgatcatg gcgaggccga agagaagaag
     9241 tgcgtcatgc gcgtggccgg cggccgcaat gccgggaaag tcgagcgcgc cggtggcccc
     9301 gtacacgagc gcagcaccga acagcagcac ggcggagccg aacccgccga tgacgaggta
     9361 cttcagcgcc gcctccagtc cgctcagccg gtcgcgatcg atcgcgcaga ggatgtagag
     9421 gcagatcgag aaccactcaa gtcccaggaa gagtgtcagc aggttgttcg cgccgacgaa
     9481 gaacaccatt ccgccgccgg cggcggcaag aagcgcgtag tactcgccga tgtgatcctc
     9541 ccgccagcgc tcgcggtacg agacggcggt agcgaggatc cccgagcccg cgacgatgat
     9601 cgcagtgagc tcggcgaggc ggtcgcggcg gaacgcgtcc acgacgacgc cgtgtccgtg
     9661 cgcagacttc acgtagagcg cgatcgcgaa gccgagcgcc gtcgcgtacc cgagcgtgca
     9721 gagggttgcg gtcacggctt tgcgcgagcg ggccggtgcc agaacggcgc agagcaacgc
     9781 gagcgcggaa gcagccagca gcgcgttgac gggggccaac gcgaaccagt cgatgtgcgg
     9841 acgcgggatc acttgaatcc ctgggcgacg tcggcctgcc cgtgatcgcc ccggaacgag
     9901 tgctgcgtga tcgccgccgg ccaggcggag agtgcgagca ggcaggcgag catcggtaga
     9961 acgacgccca gttcggcggg acggaggtcg agcgccgcgt cggagaccgc gggcccgacg
    10021 tcgcgatgga ggatcgcgga gacaagacgg agcgcgtatg ccgcggcgag caccatcgcc
    10081 accgcgccga ccacggacca gccccagccg tgccggaaca ctcccgcgag gatcaggaac
    10141 tcacccgcga atgccgagga gagcgggacc gcgagggtga tcacgcccgt cgtcagcagc
    10201 accgtggcga gtacggggcg cccgcgcgcc atgccaccaa gccgggagag ctcccccgtg
    10261 agtgttcgcc gttccaccat tcccgccaac aggaacagcg tcgtcgagat cagcccgtgg
    10321 ttgaccatct ggagaaccga gccgtccagt ccgaggtcgt tgacggagaa gacacccatc
    10381 gtgatcaggc ccatctgcgc gagcgacgag tacgcgatca cgcctcggat gtccggtgcg
    10441 cggaacgcga gcagcgagcc gtagatcagc ccgatggcgg cgagcgcgag gatgggcgcg
    10501 cgatagtgct gcgccggctc cgggaacttc gtgatcgcga tgcggagaaa gccgtaggcc
    10561 gcggccttgg agacgactcc cgagaggatc gccgccacct ccggcgagga ctcgcggtac
    10621 gcgtccggca gccagccgtg cagcgggaag agcggcgctt tgatcgcgaa tgcggcgacg
    10681 aagccgagga agacccagtc gctcgtgctc tgcccgatct gaacgaggtc gaacgtgttc
    10741 tgcgacagcc cgtagacgac gatcgaggcg agcatcagca atgaccccgc catcgtgaag
    10801 agcacgaact tcagggtggc gcccagccgc ccggggccgc cccagacgcc gacgagcacg
    10861 tagagcggga tcatcatcgc ctcgaagaag gcgtagaaga ggaggaggtc ctgcgccgtg
    10921 aagacgccga tgacggcgcc cgtcaggaag agcatcagtc ccatgtacgc gcgcatgcgg
    10981 tcgcggcccg cccagaagcc gtaggcgatc gcggcggcca tcgcgacgac cgtcagcccg
    11041 atcagccaca cggagaagcc gtagacgccg acgtggtacg agacgtgcag gtcgctgaac
    11101 caattccgct gctgcgcgaa ctgcaggccg ccctgcgcga agtcgtagcg ggcgacgagc
    11161 tggatccaga cgccgacctc cgcgagggcg gccagaagcg ccagcgcacc ggccgagacg
    11221 tcgttgagcg gcagcaccca gatgaccagc gcggccgcga gtgggaggac gatcagggcc
    11281 gtcgtcactt acctgaccgc cacgaagacc agggcgagca cggcgacgct cgaggcgatc
    11341 gcaagcgcgt aggtgcgcaa gagacccgtc tgggcccgcg acacgagacg gcccaggtcg
    11401 cgcgtgtcct gccccagcga gttcgaggac tcgtcgacca gcgggtcctc gacccagcgg
    11461 tacaagccct tcgcggccca cacggtcggc cggtagaaga gcgcgtcgta gagctcgtcg
    11521 aagtagaact tgtgctcgag cacgcgctgg gcgaacgcga agcgcggtac tgcgaccctg
    11581 cgcgcgccgt agatcagcca ggcgaggaaa atgccgccga gacccaggag cagcgcgagc
    11641 gccgacgaga ccgcttcctg ccagctcttg gggctgacgg tcggttccgc aaccgtctgg
    11701 agccaggtct cgatcgggtg ccagaggggc tcccactgga tccagccgcc gaccaccgag
    11761 aggaccgcga ggatcgcgac cggcaccgcg agcgagacgc cgacgacgtc gcgcttcagg
    11821 gcgtggaagt gctcgcgcac gaacgggctc ggctcgcccc agaagacgag gaagaggagc
    11881 cggaaggtgt agaggccggt gaggaacgtc ccagccagcc cggcagccca gaggacgtag
    11941 ccgtaggcgc cgtgatccat cgccgctgca aggatcgagt ccttcgagaa gaagccggcg
    12001 aacgggaaga tgccgacgag ggcgacggac ccgatcaaga aggcgagata ggtgcggggc
    12061 atcaactgac gcagcccgcc catcttgcga atgtcctgct cgtccgcgag ggcgtggatg
    12121 acgatgccgg ctgccatgaa gagcagcgcc ttgaagaagg cgtgcgtcat cagatggaac
    12181 atcccgtttg cgtaggcccc aacgccgacg ccgaggaaca tgtagccgat ctgcgacatc
    12241 gtcgagtatg cgatcactcg cttgatgtcg acctggacga gcgcgatcag acctgcgagg
    12301 agcagggtgg cggcgccgag gccggcggcg aggtcagcga cgtgcggggc cgcctcgaag
    12361 atcgagtgcg tgcgcacgat caggtacacg cccgcggtga ccatcgtggc cgcatggatg
    12421 agggcgctga ccggagtagg accttccatc gcgtccggca gccacgtctg cagcggcagc
    12481 tgcgccgact tggcgacagc acctccgagc agtcctagcg cgacgagtgt cgcgacggtc
    12541 gagctgtact ggccgcccag gattccgaac tcgaccgacc ccgccttctt gatcaggagg
    12601 aagagcgcga gcgccatcgt cgcgtcgccg atcgcgttca tgatgaaggc cttcttcgcg
    12661 gccgcgatcg cgctcggccg ctcgtgccag aagccgatca gcaggtacga cgagagaccg
    12721 accatgcccc agccagcgag caggagcagc aggttcccgc cctggacgag caggagcatc
    12781 gagaagacga acagcgccat gtacgcgaag tagcggcgct cctcgtcgtc gccgtccatg
    12841 tagccgatcg agtacgcgac gatcagcgcg ccgacgccgg cgacgatgag catcatgaag
    12901 acggagagcg ggtcgatcat gacctcgaag ccggagcggt acgagccccc agagatccac
    12961 gtccacgcgg tcgagacgac gacccggtgt ccaccgtcgc gcccgagcag gccgacgaag
    13021 ctcgcgatcg cgccgccgaa ggccacgaag cacgacagcg ttgcgaggta accggcgccg
    13081 cggcgcgaga tccgctcgcc cgcaagcgtg atcagccctg cggcggcaag cggcgacagc
    13141 aggcagatcc aggcgccgga gatcatccgc gcagcgagct gagccggtcg acgtcgagcg
    13201 gaatgcgctt gcggttgagc gcgacgatca ggccgagacc gaccacgact tcggaggctg
    13261 cgacggccat caccgcaagg gcgaagatct gcccgtcacc gttgttcaag ttgcgcgcga
    13321 aggcgatcag cgccaggttc ccgccgttca gcatgatctc gagtgagagc aggacgatga
    13381 gcggattacg ccggatcaga actccgagcg cgccgacgcc gaacaggaac cccgcgacgc
    13441 cgaggtacca ggcggtgccc ggccccatca cagcggcggc acctcttcgg cggtcgtgcg
    13501 cgaatgcgct cccaggatga cgcctccgat cgcggcgacg agcaggacga tcgaggtgat
    13561 ctcgaaggcg agcaggtggt cggtcaggaa gaggcggccg atgtagctcg gcgttccgaa
    13621 ggcggctccg acgtggtgcg aatgcgcgag cgcgccaccc gccttgagac cgatcacgac
    13681 cacgatctcc accagcagcg cgccggcggc caaaactgcg ccgatcgtct gccagctagg
    13741 cccgcccgtc cagggcgaat ccgtgcgcgg gccgacgtaa gcgatgacga agaggaacat
    13801 caccatgacg gcgccggcgt agacgagcac ctgcgctgcg gcgacgaact ccgatgagag
    13861 cagcaggtac aggacggcga gcgagccgag attgccgatc agtgccagcg ccgagtagaa
    13921 gggattcgtg aacgagacga ccgccaccgc cgaggcgaga cacgcgaacg ctgcacccag
    13981 ccacaccgcc cacacgagca cgttgccgac cgacgcgacg atcacgagtt gtccttgtag
    14041 tacgggatcg gcgtgtcgta gaggccgcgg tcggcaaccg ggatcttctt gatcggcccc
    14101 gcgagcagca tgtccttcgt gtagatgagg tcgtcgcggg agtactcgga gatctcgtat
    14161 tcgttgccga gcgtgatcgc gtcgaacggg cacgcgagct cgcagtaacc gcagaagatg
    14221 cagcggctca tgttgatctc gtagatgcgc gcgtagcgct cgcccgggga gacgcggttc
    14281 tccggcgtgt tctcgtccgc gacgacgcgg atgcagtcgg cggggcaggc cgctgcgcag
    14341 agcgagcagc caacgcactt ctcgagcccg ttctcatgca ggtgcaggcg gtggcgtccg
    14401 cgaaagcgcg ggtagaccgg ccgcttgtac tccgggtact gctgcgtgat cggcttcttg
    14461 aagagctgcc ggaaggtgac ggcgaagccc ttgacgctgt cgaggggttg tgggctcatg
    14521 aaacccagac gaccaggagg gcggtgatca ctgcgttgat cgtcgccacg ggtaagagga
    14581 tcttccagcc gaaccgcatc agctggtcgt atcggagccg cggtacagac gcgcggatcc
    14641 agatgaagac cgagatgaag atgaagacct tgaggaggaa ccagagcggc cccaggggtg
    14701 cgtacgggcc gtgccagcca ccgaagaag
//
LOCUS       DASVEU010000096         8506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1230792,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000096 DASVEU010000000
VERSION     DASVEU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230792"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VF225_03665"
     CDS             <1..822
                     /locus_tag="VF225_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144382.1"
                     /translation="DLEVLAPFPGWDVLERVQSKRAQLEEAATVGVDVPRTRYPRRPA
                     DARGAAEEIGLPVLVKPEHPIGFKSRFRRQAFRCETLDEVEDAYTRAEEFAPMVQELV
                     PGGDDTLYTIGSYLTRDGRPLGIFSGRKLRQTPPGIGTCRVGEAVWVQDAVDSALRLL
                     EGFGYFGLSQVEFKRDPRDGKFKLMEINARLWQWHGLAAACGIDLPRIAYADLVGEPP
                     PAATMNGEGKRWAITLLPGERPALQRPPYVDAVFARDDPKPAAVHLARVVRSALR"
     gene            819..2009
                     /locus_tag="VF225_03670"
     CDS             819..2009
                     /locus_tag="VF225_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7144383.1"
                     /translation="MIPAAIERRARWVLDSIGATDVGFGDDLPYDADAWERVERGERP
                     DDDVAEGFFHLARVEERNGGPRDEHGRFRAEWSCLDPLDPPLERLRRKLGVADADGPR
                     FTVALTHDVDTPWRWTRIGVRGAAARLKQNVRHVRLGPAVRETTGLVMAPVHFARGTD
                     PNWRFEQIVAEERRRGAAGSSFYVIASHHDPHDGAAPEEYSRLRPRLVETLLDTGAEV
                     GLHGSYAAADDPVRLAAEKHKLEALAGPILGHRYHYLRVDPHRNLAPLAAAGLRYDTT
                     LGFPDAVGFRAGIARPFHPWDFERDEPLDLVEIPLAAMDATLAEERYLGLAAKRAEPQ
                     LMRLLDWAAEYGGAFAVLWHPDRFDPVTSGGWDRLYSRLLDGVRERGGRCVPAAELAS
                     SHST"
     gene            complement(1925..3016)
                     /locus_tag="VF225_03675"
     CDS             complement(1925..3016)
                     /locus_tag="VF225_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7144384.1"
                     /translation="MRDITVLVTASGAPGTAALLRALRLNGERGVRIVGTDMSPQAVG
                     RHFCDRFYVVPPGKDPAFGDAMLDVVRRENVDAVLPQSSHDLQGLADARERFMGTTVM
                     VSPPETIRIANDKAETYALLERLGLRAPAWRRVSGGRALALAAEELGYPERAVCFKPI
                     FSSGSRGFRVLDATVDRTRQLIDERPGNLAMRLKDVMELLPEEGGPDLLLMELAQGRE
                     RTVDGIARSGQVLLGHAKTRQSMRAGLAMYFETLQDHWLMEVAQRIVAELGIEYFFNI
                     QLVGDHVIEINPRISTIVYQEDLNLAYLGIKSALGEISHEELMQLSAQVRPTRRALRY
                     FDQVEWDDASSAAGTHLPPRSRTPSSSLE"
     gene            complement(3099..3461)
                     /locus_tag="VF225_03680"
     CDS             complement(3099..3461)
                     /locus_tag="VF225_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SSI family serine proteinase inhibitor"
                     /protein_id="HEX7144385.1"
                     /translation="MNILAAILVVASTSLHITVWPDGTGQPAKKVYTLNCAPVGGTLP
                     KRAAACTKLMRATRPFAPTPQGMACTMIYGGPQEALVTGHFRGKLVRARFSRKDGCEL
                     ARWNRVLFLFPGASANSR"
     gene            complement(3458..4894)
                     /locus_tag="VF225_03685"
     CDS             complement(3458..4894)
                     /locus_tag="VF225_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7144386.1"
                     /translation="MLARLRELLRHSAIYGLGSIVARVLGVLLLPLYTRYLSPSDYGL
                     IETLVALSAVLTALVAQGMKSAFFRFYFDSAEPAHRHLVVRTAFWYVLAASTAVTAAG
                     IALAAQVSWLLFGTHGHRDLVIAAFIGLWAALNYEQMTSLFRVEQRSTAYVAATLANV
                     AITITATVLLVVVFDKGPLGVLVGNFTGTLIAYAALLLYSRHALGLQFDRPLYRAMNR
                     FGLPLVPSAVALWLTNFSDRFFLIKLADAHEVGLYSIGVRIASAIVLLLTAFRMAWPA
                     FAYSIEDDREAQRTYSFVLTYVVFVCCWLALALGLLAPWIVKLITTKPFYPAQNVVAP
                     LAFGVAAFGAYVVVQIGTGRARQTRSNWVVTGAAAAVNVALNLALIPAYGRMGAAIAT
                     VVAYTLLFVGMAWRAQRVFPVSYQWRRVATLGLAAVGLTVFGKVLDVPLPVALALTAA
                     FPLVLLVLGFYLPVERQRLRRLLPILGR"
     gene            complement(4897..5814)
                     /locus_tag="VF225_03690"
     CDS             complement(4897..5814)
                     /locus_tag="VF225_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7144387.1"
                     /translation="MNLAGTGWAHVNALRRKGVDARLLVFWPQKWRPEEYDINLDLPR
                     SGLLRQQAVQWRALARYLPQADIFHFYFGKTLVPKSLNLPILRAARKKSVMQFLGDDI
                     RGKTLHELEYRKKFDAWIVGSYAATRWVPDATAVIPPGLDLRDYEPVPPVERERPLVV
                     HAPSDLEKKGTRHVIAACAQLPVDLDVVHGVPHQEAVERFKRADIVVDQMHYLWHGVF
                     AIESMAYGKPVVTHLDPTAVAQTEEAFGLKVPIVSATKDDLVDQLRPLVDSFELRKRL
                     GEEGRAYVKRVHDLDKVADRLIEIYASIL"
     gene            5867..6643
                     /locus_tag="VF225_03695"
     CDS             5867..6643
                     /locus_tag="VF225_03695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144388.1"
                     /translation="MSAGLKRRLYRTARDLRHPGPALERRRIRDGVARFLGGRHALVG
                     YEREVRDSGLMEHLLEKGREHHEAVVAAGAGHSLGAIGYTEGVYLYAVLRTVRPDVVV
                     ETGVANGFSTAFSLLALQANEAGHLHSIDLPREVGRDYEPGTFYEGEGRAGIPPGSEP
                     GWLIPQALKERWTLIFGRSQEELPPLLEQLGTVDVFMHDSEHSFDCMWFEFNAAWPAL
                     RPGGVLISDDVNSTQAFGQFAVQEGRDPVRLARGMALLRK"
     gene            6681..7232
                     /locus_tag="VF225_03700"
     CDS             6681..7232
                     /locus_tag="VF225_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7144389.1"
                     /translation="MAVAERKSAEERREQILEAALVEFAARGLVGGSTEAIAKTVGIS
                     QPYVFRLFGTKKELFIATVELCLRGTLEMFRAASAGLHGEEALHAIGEAYVERLASDP
                     TYLRSQMQAYAASDDQQIRDVVRKGYGELVEYVERVSGEPSDRVSHFFAKGMLLNVVA
                     SLDLLGAEEGWAQRLIEGCRQDV"
     gene            7294..>8506
                     /locus_tag="VF225_03705"
     CDS             7294..>8506
                     /locus_tag="VF225_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7144390.1"
                     /translation="MSAKTRTMWTFAITSVAVFMATLDNLVVTTALPVIREDLHATVE
                     SLEWTVNAYTLTFAVLLLTGAALGDRFGRRRMFVVGLAIFTVSSAAAALAPSAGALIA
                     ARAAQGVGGAIITPLTLTILSAGVPANRRGAFIGAWSGIAGLAVALGPVVGGAVVSGI
                     SWHWIFWLNVPIGIVLIPLALRRLDESHGPAAKLDLPGVGLASAGLTGIVWALVRGNG
                     QGWGSAEIVASLIAGAIVLALFILWELRVEEPMLPMRFFKNRVFALANVASLFMFFGM
                     FGSIFLLSQFFQIIQGYSPLGSGVRILPWTIMPLFVAPVAGALSDRIGGHRLMGVGLT
                     LQAAGLGTIAAISTPTTPYWQLVAPFAISGIGMAMFWAPVANVVLSAVKPEEQGQASG
                     ANNAIRELGGVF"
BASE COUNT         1371 a         2807 c         2931 g         1397 t
ORIGIN      
        1 gatctcgagg tgctcgctcc tttcccgggc tgggacgtcc tcgagcgggt tcagagcaag
       61 cgcgcccaac tggaagaagc ggcgaccgtc ggagtcgacg tgcctcgaac gcgctatccg
      121 cgcagacccg cggacgcgcg cggagctgca gaggagatcg gtctaccggt gctcgtcaag
      181 ccggagcatc ccatcggctt caagtcgcgc tttcgccgcc aggccttccg ttgtgagacc
      241 ctcgacgagg tcgaggacgc ctacacgcgc gcagaggagt tcgcgccgat ggtccaggag
      301 ctcgttcccg gcggcgacga cacgctctac acgatcggca gctacctcac gcgcgacggg
      361 cggccgctcg ggatcttctc cggtcgcaag ctgcgacaga cgccgcccgg catcggcacc
      421 tgccgcgtcg gcgaggcggt ctgggtgcag gacgccgtcg actcggcact gcggctgctc
      481 gaagggttcg gctacttcgg gctgagccag gtcgagttca agcgcgatcc gcgtgacgga
      541 aagttcaagc tgatggagat caacgcacgc ctgtggcaat ggcacggtct cgctgcggcc
      601 tgcggcatcg acttgccgcg catcgcctac gcggacctcg tcggcgagcc gccgcccgcc
      661 gcgactatga acggcgaagg caagcgctgg gcgatcacgc tgctcccggg cgaacgcccg
      721 gcgctccagc gtccgccgta cgtcgacgcc gtcttcgcgc gggacgaccc gaagcccgcc
      781 gccgtccacc tggcgcgggt tgtgcggagc gcgcttcggt gattccagcg gcgatcgagc
      841 gtcgtgcgcg ctgggtcctg gattccatcg gcgcgaccga tgtcggcttc ggtgacgatc
      901 tgccgtacga cgccgatgcg tgggagcgag tcgagcgcgg cgagcgtccc gacgacgacg
      961 tagccgaagg cttcttccac cttgcgcgcg tcgaggagcg gaatggcggc ccgagggacg
     1021 agcacggccg cttccgtgcc gagtggtcgt gtctcgatcc gctcgacccg ccgctggagc
     1081 gattgcgccg caagctcggc gtcgccgacg ccgacggtcc gcggttcacg gtcgctctga
     1141 cccacgacgt cgacacgccg tggcgctgga cgcggatcgg cgtgcgcggc gcagctgcgc
     1201 ggctgaagca gaacgttcgg cacgtccgcc tcgggcccgc tgttcgcgaa accacggggc
     1261 ttgtgatggc gcccgtccat ttcgcgcgcg gcaccgaccc gaactggcgg ttcgagcaga
     1321 tcgtcgcgga agaacgtcgc cgcggcgcgg ccggctcgtc gttctacgta atcgcgagcc
     1381 accacgaccc gcacgacgga gcagccccgg aggaatacag ccggctccgg ccgcggctcg
     1441 tcgagacgct gctcgacacc ggtgccgagg tcggcctcca cggcagctac gccgcagccg
     1501 acgaccccgt ccgcctcgct gcggagaagc acaagctgga ggcgctcgcg ggcccgatcc
     1561 tgggccaccg ctaccactac ctccgcgtgg atccacaccg aaatctcgct ccgctcgcgg
     1621 ccgccgggct tcgctacgac acgacgctgg ggttcccgga cgcggtcggc ttccgcgccg
     1681 gcatcgcccg cccgttccac ccgtgggact tcgagcggga cgagccactc gacctcgtcg
     1741 agatcccgct cgcggcaatg gatgcaacgc tcgcggaaga gcgctacctc ggattggcgg
     1801 cgaagcgcgc cgagccgcag ctgatgagac tcctcgactg ggccgccgag tacggcgggg
     1861 cattcgccgt cctctggcac ccggaccgct tcgacccggt cacctccggc ggctgggacc
     1921 ggctctactc caggctgctg gacggagtcc gggagcgcgg gggcaggtgt gtcccagcag
     1981 ccgagctcgc gtcgtcccat tcgacttgat cgaagtagcg cagcgcacgg cgcgtgggac
     2041 gcacctgtgc gctcagctgc atcagctctt cgtgtgagat ctcgccgagc gcgctcttga
     2101 tcccgagata cgccaggttg aggtcttcct ggtacacgat cgtggagatg cgcggattga
     2161 tctcgatcac gtggtcgccg acgagctgga tgttgaagaa gtactcgatc ccgagctcgg
     2221 cgacgatgcg ctgcgcgact tccatcagcc agtgatcctg cagcgtctcg aaatacatcg
     2281 cgaggccggc acgcatcgat tggcgtgtct ttgcatgtcc gagcaggacc tggcccgagc
     2341 gggcgattcc gtccactgtt cgttcgcgac cctgtgccag ttccatcagc aggaggtccg
     2401 gcccgccctc ttccggtagg agctccatca cgtccttgag cctcatggcg aggttccctg
     2461 ggcgttcgtc gatcagctgt ctcgttctgt ccacggtcgc gtccagcacg cggaagcccc
     2521 gtgagcccga gctgaagatc ggcttgaaac agaccgcgcg ctcggggtac ccgagctcct
     2581 cggcagcgag ggcgagcgcg cggccaccgc tcactcggcg ccaggcaggt gcgcgcaggc
     2641 cgagacgctc gagcagggcg taggtctccg ccttgtcgtt cgcgattcgg atcgtctccg
     2701 gcggcgacac catcaccgtc gtgcccatga agcgctcgcg cgcgtccgcg agcccttgca
     2761 ggtcgtgaga cgactgcggc agcacggcat cgacgttctc gcgccgcacg acgtcgagca
     2821 ttgcgtcgcc gaacgccgga tctttgccgg gcgggacgac gtagaagcgg tcgcagaagt
     2881 gccggccgac cgcctgcgga gacatgtcgg tgccgacgat gcgcacgccg cgctcaccgt
     2941 tcaggcgcag cgcccgtagg agcgccgccg tgccgggagc gcccgacgcg gtgacgagga
     3001 cggtgatgtc gcgcatcgaa gcggcgatgc tacttggcgt ctatccctgg tggatcggtt
     3061 tgagcatcgc ttcggcgtcg agttcgagcc ccaggttctc agcggctgtt cgccgaggcg
     3121 ccagggaaga gaaagaggac gcggttccag cgggcgagct cgcaaccgtc cttacggctg
     3181 aagcgcgccc ggactagctt cccgcggaag tggccggtga ccagcgcctc ctgggggccg
     3241 ccgtagatca tcgtgcaggc catgccttgc ggcgtcgggg caaacggtcg tgtggctcgc
     3301 atcagcttcg tgcacgcggc tgcgcgtttc ggcagcgttc ctccgaccgg ggcgcagttg
     3361 agcgtgtaga ccttcttcgc cggttgcccg gttccatcgg gccacacagt gatgtgcagc
     3421 gatgtcgagg cgacgacgag gatcgcggcc aggatgttca tcgtcccaga ataggcaaga
     3481 gccggcgtag gcgctgccgc tctaccggta ggtagaagcc gagcacgagc aacacaagcg
     3541 gaaatgccgc agtgagcgca agcgcgaccg ggagcgggac gtcgagcact ttcccaaaga
     3601 cggtgaggcc gacggccgcg agcccgagcg tcgcgacacg ccgccactgg tacgacactg
     3661 ggaagacgcg ctgtgcgcgc caggccatgc cgacgaagag aagcgtgtag gcgacgaccg
     3721 ttgcgatcgc ggctcccatc cgtccgtagg ccggaatgag cgccaggttc agcgcgacgt
     3781 tgaccgcggc ggccgcgccg gtcacgaccc agttcgaacg cgtctgccgc gctcggccgg
     3841 tgccgatctg aaccacgacg taggcgccga acgcggcgac gccgaacgcg aggggtgcga
     3901 ccacgttctg tgccggatag aacggcttcg tcgtgatcag cttcacgatc cacggcgcca
     3961 gcagtccgag cgcgagcgcc agccaacaac agacgaagac gacgtacgtc agcacgaagc
     4021 tgtacgtgcg ctgcgcctcg cggtcgtcct cgatcgagta cgcgaacgcc ggccaggcca
     4081 tcctgaatgc agtgaggagc aggacgatcg ccgacgcaat gcgcacgccg atcgagtaga
     4141 ggccgacctc gtgcgcgtcc gcgagcttga tcaggaagaa gcggtcgctg aagttggtca
     4201 gccagagcgc gacggccgag gggacgagcg gcaggccgaa gcggttcatc gccctgtaga
     4261 gcgggcggtc gaactgcagg ccgagggcat gcctgctgta gagcaggagc gccgcgtacg
     4321 cgatcagcgt cccggtgaag tttccgacca gcacgccaag gggccccttg tcgaagacga
     4381 cgacgagcag gacggtggcg gtgatcgtga tcgcgacatt cgcgagcgtt gccgcgacgt
     4441 aagccgtcga gcgctgctcg acgcggaaga gggatgtcat ctgctcgtag ttcagcgcgg
     4501 cccacaggcc gatgaacgcc gcgatgacga gatcacggtg gccatgcgtg ccgaagagga
     4561 gccacgagac ctgcgccgca agcgcaatcc ctgcggccgt cacagcggtg gacgcggcca
     4621 gcacatacca gaacgctgtg cgcacgacga ggtgtcgatg cgccggctcg gcggagtcga
     4681 agtagaaccg gaagaacgcg ctcttcatcc cctgtgcgac gagagccgtc agcacagccg
     4741 acagtgccac gagtgtctcg atcaacccgt agtcgctcgg cgacaggtag cgcgtgtaca
     4801 gcggcagcag cagcacgccg agcacgcgcg cgacgatcga gccgagcccg tagatcgccg
     4861 agtgacgcag gagctccctg agccgcgcaa gcatgctcaa agtatggagg cgtaaatttc
     4921 gatgagccgg tcggccacct tgtcgaggtc gtgcacccgt ttcacgtaag cgcgaccttc
     4981 ctctccgagc cgcttgcgta gctcgaagga gtcaacgagc gggcgaagct ggtccacgag
     5041 atcgtccttc gtcgcggaga cgatcggcac cttcaggccg aaggcctctt cggtctgggc
     5101 aacggccgtg ggatcgaggt gcgtgacgac gggcttgccg tacgccatcg actcgatcgc
     5161 gaacacgccg tgccacaggt agtgcatctg gtcgacgacg atgtctgcgc gcttgaaccg
     5221 ttccaccgcc tcctggtgtg gcacgccgtg cacgacgtcg aggtcgaccg gcagctgcgc
     5281 gcacgcggcg atcacgtgcc gcgttccttt cttctcgaga tccgagggcg catgcacgac
     5341 gagtggccgc tcacgttcga ccggcggtac aggctcgtaa tcgcgcaggt cgagccccgg
     5401 cgggatgacc gctgtcgcgt ccggaaccca gcgcgtcgcg gcgtacgaac cgacgatcca
     5461 ggcgtcgaac ttcttgcggt actcgagctc gtgcaaggtt ttgccgcgga tgtcgtcgcc
     5521 gaggaactgc atcaccgact tcttccgcgc cgcgcgcagg atcggcaggt tcagcgactt
     5581 cgggacgagc gtcttcccga agtagaagtg gaagatgtcc gcctgcggga gatagcgagc
     5641 gagggcgcgc cactgcacgg cctgctggcg gagaaggccc gaccgcggca ggtcgaggtt
     5701 gatgtcgtac tcctccggcc gccacttctg cggccagaag acgagcagtc ttgcgtccac
     5761 gcccttgcgc cgcagggcgt tcacgtgggc ccagccggtg ccggcgaggt tcacgggaag
     5821 gtggaggacc cgggccgtca gaagcccaac gatacgatcc gcggccttgt ccgccgggtt
     5881 gaaacgacgt ctctatcgga ccgcgcgcga tctgcgtcat ccaggtcctg ccctcgaacg
     5941 ccgccgcatc cgcgacggtg tggcgcgttt tcttggcggc cgccacgcgc tcgtcgggta
     6001 cgagcgggag gtgcgtgaca gcgggctgat ggagcacctg ctcgagaaag ggcgggagca
     6061 tcacgaagcg gtcgtcgcag cgggcgccgg ccattcgctc ggcgcgatcg gctacacgga
     6121 gggcgtctac ctctacgcgg tcctacggac ggtccggccg gacgtggtgg tcgagaccgg
     6181 ggtggcgaac ggtttctcga ccgccttctc gctactggcg ctgcaagcga acgaggcagg
     6241 ccacctccac tcgatcgatc tgccccgcga ggtcggccgc gactacgagc cgggaacgtt
     6301 ctacgagggc gaaggccgtg cgggcatccc gcccggctcc gagccgggct ggctgatccc
     6361 acaggctttg aaggaacgct ggacgctgat tttcggccgc agccaggagg agctgccgcc
     6421 gctgctcgaa cagctgggaa cggtggacgt cttcatgcac gacagcgagc actcgttcga
     6481 ctgcatgtgg ttcgagttca atgccgcttg gcccgctttg cggcccggcg gcgtactgat
     6541 ttcggacgac gtgaactcga cccaggcatt cggccaattc gccgtacagg aggggcgcga
     6601 tccagtccgc ctcgcccgtg gaatggccct gctgcggaag tgatttgaca agttagtgac
     6661 tagtagctaa catcctcgcg atggcagttg cagagcgaaa gagcgccgag gaacggcgcg
     6721 agcagatcct cgaagcggcc ctggtcgagt tcgccgcccg ggggctggtc gggggttcga
     6781 cggaggcgat cgcgaagacc gtcgggatct ctcagcccta cgtcttccgc ctcttcggca
     6841 cgaagaagga gcttttcatc gcgaccgtcg agctctgcct gcgcgggacg ctcgagatgt
     6901 ttcgcgcggc gtccgccggc ctccacggcg aggaggcctt gcacgcgatc ggcgaggcgt
     6961 acgtcgagcg actggccagc gatccgacct atctgcgttc gcagatgcag gcctatgcgg
     7021 cgtcggacga tcagcagatc cgcgacgtcg tgcggaaggg ctacggcgag ctcgtcgagt
     7081 acgtggaacg cgtttcgggc gagccctcgg accgcgtgtc ccatttcttc gcgaagggga
     7141 tgctcctgaa cgtcgtcgcc tcgctggacc tactcggcgc cgaggagggg tgggcgcagc
     7201 gcctcatcga gggctgccgc caggacgtct agtcattttt ttcaccttga agtaagtgat
     7261 cactcacaag catgagagcc gaaggaggaa tccatgagcg ccaagactcg aacgatgtgg
     7321 actttcgcaa tcacctcggt ggcggtgttc atggccaccc tcgacaacct cgtggtcacg
     7381 accgcgctgc cggtgatccg cgaggacctg cacgcgaccg tcgagagcct cgaatggacc
     7441 gtcaacgcgt acacgctcac cttcgcggtg ctgcttctga ccggagccgc gctcggcgac
     7501 cggttcgggc ggcgccggat gttcgtggtc ggtcttgcga tcttcaccgt ctcgtcggcg
     7561 gcggctgccc tcgcgccctc tgccggagcg ctgatcgcgg cgcgtgcggc tcagggcgtg
     7621 ggcggcgcga tcatcacgcc gctgacgctc acgatcctca gcgcgggcgt gcctgccaat
     7681 cgccgcggtg ctttcatcgg cgcctggagc ggcatcgccg gcctggcggt cgcactcggc
     7741 ccggtcgtcg gcggtgccgt cgtgagcggc atctcgtggc actggatctt ctggctcaac
     7801 gttccgatcg gcatcgttct gattccgctc gcactgaggc gtctcgacga gtcgcacggg
     7861 cctgcggcca agctcgacct gccgggcgtt gggctcgcca gcgccggtct gaccgggatc
     7921 gtgtgggccc tcgtccgcgg caacggacag ggttggggat cggcggaaat cgtcgcctca
     7981 ctgattgccg gtgcgatcgt gctcgcgctg ttcatcctct gggagctccg tgtcgaggag
     8041 ccgatgctcc cgatgcgctt cttcaagaat cgcgtcttcg cgctcgcgaa cgtcgcgtcg
     8101 ctgttcatgt tcttcggcat gttcggctcg atcttcttgc tgagccagtt ctttcaaatc
     8161 atccagggct acagcccgct cgggagcggc gttcgcatcc tgccgtggac gatcatgccg
     8221 ctcttcgtgg cgccggtcgc cggggctctg tccgaccgga tcggcgggca ccggctcatg
     8281 ggcgtgggcc tgactttgca ggcggcaggg ctcgggacga tcgcggctat ctcgacgcct
     8341 accacgccct actggcagtt ggtcgcgccg ttcgcaatct cgggtatcgg catggcgatg
     8401 ttctgggcgc ccgtcgcgaa cgtcgtgctc tcggcggtga agcccgagga gcaagggcag
     8461 gcgtccgggg cgaacaacgc catccgggag ctcggaggag tcttcg
//
LOCUS       DASVEU010000097         7219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_648889,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000097 DASVEU010000000
VERSION     DASVEU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648889"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VF225_03710"
     CDS             <1..270
                     /locus_tag="VF225_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144391.1"
                     /translation="NRIAEVLGIDPLQLRLKNANRIGDTTPNRVVLKDPSTVPVVLAA
                     ADRVGYDLPDEYKSMTNERRTGDLLPEHLVAQLGGPESASNGGGR"
     gene            270..1370
                     /locus_tag="VF225_03715"
     CDS             270..1370
                     /locus_tag="VF225_03715"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7144392.1"
                     /translation="MAKHKGRGIATIEYPTGMNQNGDPSQAWVKLKPDGRVDVFSGTV
                     DIGQGSRTVHTQIVADALGVPFDWVTNDNTNTDSSAVCTGSFASRGTFIGGNAVRFAA
                     EQARERILDIASKELEIAPGDLDIVDGEVVAKGAPDRKIGIPEVAAAATWNYGELITG
                     TGAALKPYADVNDDDGSVEIEPHSAISYAACVAEVEVDDETGEVTVERLIQVYDVGRA
                     INPTLVEGQIEGGAMMGLGLGLLEEAYPYYPETDHRGGEFGAYLAPGLEDLPDVDNLI
                     IENPSEDGPFGAKAIGEMANNAQPPAIVSAIYDAVGAWVTQLPATPERVLRALDGEKE
                     PRRDGKRVIFDDPVSIRTVSSDGGKGFFETDG"
     gene            1378..1761
                     /locus_tag="VF225_03720"
     CDS             1378..1761
                     /locus_tag="VF225_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144393.1"
                     /translation="MPFRTWNGVAAWQFVDGVTLHAIGGEQILLCRVTYEPGKQVPDH
                     AHEHTEQVMAIVDGEATVTVAGETRDVKAGDVVVINRGVRHSVSSEKGLTFFEALAPV
                     PLDHVPDRDRDLVLGPDGGSGHVER"
     gene            1787..1969
                     /locus_tag="VF225_03725"
     CDS             1787..1969
                     /locus_tag="VF225_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7144394.1"
                     /translation="MPMIEVKLYDHRVTDESVPKMIEALTDALHESSGAAKEHIHVVI
                     QGVQPKHWGIAGKPAG"
     gene            1974..2177
                     /locus_tag="VF225_03730"
     CDS             1974..2177
                     /locus_tag="VF225_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7144395.1"
                     /translation="MPLIEVKLFDFRMDEETSRKLIAGLTDALCDATTEGLREHTWVV
                     VEGHSPKNWGVGGVPWPAEKTPP"
     gene            complement(2249..4507)
                     /locus_tag="VF225_03735"
     CDS             complement(2249..4507)
                     /locus_tag="VF225_03735"
                     /inference="COORDINATES: protein motif:HMM:NF011979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HEX7144396.1"
                     /translation="MSPRSRRFVERERRELRREVLITPDPELGLVAMNGPNDPEPGLK
                     IEQGRVVWLDGRGEEEFDAIDHFIASHGLDLEGAAEAMALDEAELARRLVDVDVPREE
                     LVRLARGLTPARLARVVSLLDPVEMMFALKKLRARRAPANQAHVTNLKENPALLAADA
                     AEAAARGFAEIETTVGVSRYAPLNAMAILVGSQTGRPGVLTQCAIEERRSLQLAIQGI
                     TTYAETLSVYGTEPVFVDGDDTPWSKGFLASAYASRGIKVRFTSGGGSEALMGHAGGC
                     SMLYLEARCLSLARAAGSQGVQNGAISCVALVMSVPAGSREILAENLLAAWLDLEVAS
                     GNDAIASHSPTRRTAKLMGQFLPGTDFVTSGWSVMPRYDNMFGGGNYDADDIDEWLTI
                     QRDWQVDGGIEPLSEDEAISVRERAARAIQAVFAAFDFPVITDAEVEAATYALDSRDL
                     PDRDRAADVAAADRVLAEGISGIDVARGLDRHGFTGVAEAILGMQRQRVSGDYLQTSA
                     IIDSTGAVSAAVNDPNRYSGPGTGYTLDGERWERLQHLPHELDARTLDEATDSQRAVV
                     VEKHVAIGGNEPDDVVIALGPAFADGLRTTIGGLAHQDVLEALIQGVQDAGAAPRLVR
                     VRRASDVAFIGHDGAALAGSGVAIGIQSKGTTVIHRADLQPLDNLELFGMAPSLTLDS
                     YRAIGRNAGGYALKQRVGPVPTVMDNFARAKLIVQTTLLHARETAAITPGAPAIELEL
                     VAREGTSRTARV"
     gene            complement(4491..4919)
                     /locus_tag="VF225_03740"
     CDS             complement(4491..4919)
                     /locus_tag="VF225_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HEX7144397.1"
                     /translation="MAFDPIADYPLGTQRPDLIRTPGGLGLEELTLDALRSGRLDASE
                     MRATAETLELQAQVALAAGRPQLAANLERAAELTGVPDEVILEVYTALRPHRSTADEL
                     ERWADRLEGEFQATMTAAFVREAGAVYAKRNLLLPDEPTV"
     gene            4975..5700
                     /locus_tag="VF225_03745"
     CDS             4975..5700
                     /locus_tag="VF225_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7144398.1"
                     /translation="MNAYDFGGRVALVTGGASGIGAATVELLRTSGARVAVLDVDPNG
                     LHEDVLPIRGDVTRSEDAEAAVARVDSELGGLDILVCSAGVGGDSVRAVDMPDEEWRR
                     VFAINCDGTFFCNRAAVRKMIERQYGRVVNVASIAGKEGNPNASAYSASKAAVIGMTK
                     SIGKEVAGSGVLVNCIAPAVIDTPILGQLSEEHIGYMLQKIPLGRVGKPEEVAHLIAY
                     LASENLSFATGACFDISGGRAVY"
     gene            5697..6599
                     /locus_tag="VF225_03750"
     CDS             5697..6599
                     /locus_tag="VF225_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7144399.1"
                     /translation="MIRIVRFREPGTDARLGLVAPDAPTTVAELESDDLLAVLRGARA
                     VSGRTTEALDAETLELPPPFQLLAPLVPPETWAAGVTYERSRDARVDESQVEARDVYN
                     LVYDAERPELFLKDAAGRRTVGPGAAIGVRSDAQWTVPEPELALVLDSEGELLGATIG
                     NDVTARDVEAANPLYLPQAKLFAAACALGPAIVPPEDWSQPFEIKLRIIGPAGDVVYE
                     AQTSTARMRRTFEELAAWVVRENPVPAGSVLLTGTGLVPPDDVTLVPGQQVEIRIPGI
                     GTLVNPVGAAADLLPSERSKTHVR"
     gene            6589..>7219
                     /locus_tag="VF225_03755"
     CDS             6589..>7219
                     /locus_tag="VF225_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7144400.1"
                     /translation="MSDSVLEAPARPELANYVGGEWTPARSGRTYEKRNPANPAEVVG
                     EFPASGEEDVAAAVEAAAKAFPGWSGLPAAGRAAVLMRAANAIEVRVEEMAADMTREM
                     GKPLREARMESARTAAIFRYFAGEAGRPKGELYEQSATGSTLYTLRRPLGVVGLITPW
                     NFPAAIPAWKMAPALVYGNTVVLKLAQEAPLTGLHLAAALDEAGIPEGVL"
BASE COUNT         1230 a         2430 c         2454 g         1105 t
ORIGIN      
        1 aaccggatcg ccgaggtgct cgggatcgat ccgctccagc tgcggctgaa gaatgcgaac
       61 cggatcggtg acacgactcc caatcgagtc gtactcaagg atccgtcgac cgtcccggtc
      121 gtcctcgctg ccgcagaccg cgttggctac gacctccccg acgagtacaa gtcgatgacg
      181 aacgaacgtc gcaccggcga cctgctcccg gagcatctcg tcgcccagct cggcgggccg
      241 gaatccgcgt cgaacggagg ggggcggtaa tggcgaagca caagggccgg gggatcgcga
      301 cgatcgagta ccccacggga atgaatcaga acggcgaccc gagccaggcc tgggtcaagc
      361 tcaagcctga cgggcgagtc gacgtctttt ccgggacggt cgacatcggc cagggatcgc
      421 ggacggtgca cacgcagatc gtcgccgacg cgctcggcgt ccccttcgac tgggtcacca
      481 acgacaacac gaacaccgac tcgtctgccg tgtgcaccgg gagcttcgcc tctcgcggca
      541 cgttcatcgg cggcaacgcc gttcgcttcg cagccgagca ggcacgcgaa cggatcctcg
      601 acattgcctc gaaggagctc gagatcgcgc ccggggatct cgacatcgtc gacggcgagg
      661 tcgtcgccaa gggcgctccg gaccggaaga tcgggatccc agaagtggcc gccgccgcaa
      721 cctggaacta cggcgagctg atcaccggca ccggtgcggc gctgaagccg tacgccgatg
      781 tcaacgacga cgacggctcc gttgagatcg agccgcattc cgcgatctcc tacgccgcct
      841 gcgtggcgga ggtggaggtc gacgacgaga cgggcgaggt gacggtcgag cggttgatcc
      901 aggtctacga cgtcggccgc gcgatcaatc cgacgctcgt cgagggccag atcgaaggcg
      961 gcgcgatgat gggactcggg ctggggttgc tggaagaggc gtacccgtac tatccggaga
     1021 ctgatcaccg gggcggcgag ttcggcgcct acctcgcccc gggactcgag gacctgcccg
     1081 acgtcgacaa cctgatcatc gagaacccgt ccgaggacgg gccgttcggc gcgaaggcga
     1141 tcggcgagat ggcgaacaac gcgcagcctc cggcaatcgt gtccgctatc tacgacgccg
     1201 tcggcgcctg ggtgacccag ctgcctgcga cgccggaacg ggttctgcgc gcgctcgacg
     1261 gagagaagga gccacggcgc gacggcaagc gcgtgatctt cgacgacccg gtttcgatcc
     1321 ggacggtgag ctccgacggc ggcaagggct tcttcgagac agatggctga taccgcgatg
     1381 ccgtttcgaa cctggaacgg cgtcgcggct tggcagttcg tcgacggcgt gacgttgcat
     1441 gcgatcggcg gggagcagat cctgctctgc cgggtcacgt acgagccggg gaagcaggtg
     1501 ccggatcacg ctcacgagca caccgagcaa gtgatggcca tcgtggacgg cgaggcgacc
     1561 gtgaccgttg cgggcgagac ccgtgacgtc aaagcgggcg acgtcgtcgt gatcaaccgc
     1621 ggtgtccggc acagcgtgtc ttcggagaag ggactgacgt tcttcgaagc actcgcacca
     1681 gtgccgctcg accatgtccc cgatcgggac cgtgatctcg tccttggccc cgacggcggc
     1741 agcggtcacg tcgagcgctg atctacacgt aggaaaggag caggcaatgc cgatgatcga
     1801 agtcaagctc tacgaccacc gcgtgaccga cgaatccgtg ccgaagatga tcgaggcgct
     1861 caccgacgcg ctccacgagt cgagcggtgc ggcgaaggag cacatccacg tcgtcattca
     1921 gggcgtccag ccgaagcact ggggcatcgc gggcaagccc gcgggctaga accatgccgc
     1981 tgatcgaggt caagctcttc gatttccgca tggacgagga gacgagccgc aagctgatcg
     2041 ccggactcac ggatgcgctc tgcgacgcga cgaccgaagg cctacgcgag cacacgtggg
     2101 tcgtcgtcga aggacactcg ccgaagaact ggggcgtcgg cggcgtgccc tggccggcag
     2161 agaagactcc accctgatcc gaagagctgg ggtcagaccc ttgccgttcg ggacgtgccg
     2221 ccatcaggca cggcacaaga ggctggggtc agacccttgc cgttcgggac gtgccttctc
     2281 tggctacgag ctccaactcg atggcgggcg cgcctggcgt gatagctgcc gtctcgcgag
     2341 cgtgtaacag agtcgtctgg acgatcaact tcgcgcgcgc gaagttgtcc atcaccgtcg
     2401 gcaccggtcc gacgcgctgc ttcagcgcgt agccaccggc gttgcggccg attgcgcgat
     2461 aggagtcgag cgtgagcgac ggcgccatgc cgaagagctc gaggttgtcg agcggttgga
     2521 gatcggcccg gtggatgacc gtcgtcccct tcgactggat cccgatcgcg acgccggagc
     2581 cggccagggc ggcgccgtcg tgaccgatga aggccacatc cgatgcgcgc cgcacgcgga
     2641 cgaggcgcgg cgcggcgcct gcgtcttgca ctccctgaat cagcgcctcg agcacatcct
     2701 gatgagccag gccgccgatc gtcgtccgca atccgtcggc gaacgccggc ccgagcgcga
     2761 tcacgacgtc gtccggttcg ttgcctccta tcgcaacatg cttctcgacg actacggccc
     2821 gctgcgagtc cgtcgcctcg tccaacgtgc gtgcatcgag ctcgtgcggg aggtgctgca
     2881 ggcgttccca cctctcgccg tccagggtgt agccggtgcc agggcccgag tagcggttcg
     2941 gatcgttgac cgccgcgctg accgcacccg tcgagtcgat aatcgccgag gtctgcagat
     3001 agtcgcccga gacgcgctgg cgctgcatgc cgaggatcgc ctccgctacg ccggtgaatc
     3061 catgccggtc gagcccgcgc gcaacatcga tgccgctgat tccctccgcc agcacccgat
     3121 cggccgcggc aacatcggca gcacgatcgc gatcaggtag gtcgcggctg tcaagcgcat
     3181 aggtcgcggc ttcgacttca gcatcggtga tcacaggaaa gtcgaaggcg gcgaagacgg
     3241 cctggatcgc gcgcgccgca cgctcgcgca cgctgatcgc ttcgtcctcg ctaagcggct
     3301 cgatgccgcc gtccacctgc cagtcgcgct ggatcgtcag ccactcgtcg atgtcgtcgg
     3361 cgtcatagtt gccgcccccg aacatgttgt cgtagcgcgg catcacgctc caacccgacg
     3421 tgacgaagtc ggttccagga aggaactgcc ccatcaactt ggccgtgcgc cgggtcggag
     3481 agtgcgaggc gatcgcgtcg ttgccggatg cgacctcgag gtcgagccag gcggcgagca
     3541 gattctcggc gagaatctcg cgcgaccctg cgggaacgct catcacgagc gcaacgcacg
     3601 agatcgcccc gttctggacg ccctgggagc cggctgcccg cgcgagcgac aggcaacgcg
     3661 cttcgaggta gagcatggag cacccgcccg catggcccat cagcgcttcg gaaccaccac
     3721 cggacgtgaa gcgcaccttg atcccgcgcg acgcgtaggc cgaggcgaga aagcccttcg
     3781 accagggcgt gtcatcgccg tccacgaaca ccggctccgt gccgtagacc gaaagtgttt
     3841 ccgcgtacgt ggtgattccc tggatcgcca gctgcagcga ccgtcgctct tcgatcgcgc
     3901 actgcgtcag cacgcccggt ctccccgtct gggagccgac gagaatcgcc atggcgttca
     3961 gcggtgcata gcgcgagacg ccgacggtgg tctcgatctc ggcgaagcca cgcgccgccg
     4021 cctccgccgc gtctgccgcg agcagcgccg ggttctcctt caggttggtg acgtgcgcct
     4081 ggttcgccgg agcacggcgg gcgcgcagct tcttcagggc gaacatcatc tcgacgggat
     4141 cgagcaagga gaccacgcgc gcaagtcgcg caggcgtcag cccacgtgca agccggacga
     4201 gctcctcgcg cggtacgtcc acatcgacga gtcggcgggc aagctcggct tcatcaaggg
     4261 ccatcgcctc ggccgcccct tcgagatcga gaccgtggct cgcgatgaag tggtcgatgg
     4321 cgtcgaactc ctcttcaccc cgtccgtcga gccacacgac tcgcccttgc tcgatcttca
     4381 gaccgggctc cgggtcgttc ggaccgttca tggcgacaag cccgagctcg ggatccggcg
     4441 tgatcagaac ctcacgccgc agctcgcgac gctcgcgctc gacgaaccgt ctagaccgtg
     4501 ggctcatcag gaaggaggag atttcgcttg gcgtagaccg cccccgcttc ccggacgaag
     4561 gcggcggtca tcgtcgcctg gaactctcct tcgagccggt cggcccacct ctcgagctcg
     4621 tcggcggtcg aacgatgcgg gcgcagagcg gtgtagacct cgaggatcac ctcgtcgggc
     4681 acgcccgtca gctcggctgc ccgctcgagg ttggccgcaa gctgtgggcg cccggccgcg
     4741 agggccacct gtgcttgaag ctcgagcgtc tcggctgtag cccgcatctc cgaagcgtcc
     4801 agccggccgg agcgaagcgc gtcaagcgtg agctcttcga gcccgagccc gccgggcgta
     4861 cggatcaggt cgggcctttg ggtcccgagc gggtagtccg cgatcggatc gaaagccacc
     4921 ggccaagctt agtccgagcc cgcactccgt tcgccccgcg atataagact gcgtgtgaac
     4981 gcatacgact tcggcggccg cgtggccctc gtcacaggag gcgccagcgg gatcggcgca
     5041 gcgaccgtcg agttgctgcg cacgagcggc gcacgggtgg ccgtgctcga cgtcgatccc
     5101 aacggacttc acgaggacgt cctcccgatt cgcggcgacg tgacgagatc ggaggatgcc
     5161 gaggccgccg tcgctcgagt cgactccgag ctcggcgggc tcgacatcct cgtctgctcg
     5221 gcgggagtcg gcggcgactc ggttcgcgcc gtcgacatgc cggacgagga atggcggcgc
     5281 gtcttcgcga tcaactgcga cggcacgttc ttctgcaacc gcgcggccgt gcgcaagatg
     5341 atcgagcgac aatacgggcg cgtagtcaac gtcgcctcga tcgcaggcaa ggaaggcaat
     5401 ccgaacgcct cagcctactc ggcgagcaag gccgcagtga tcggcatgac caagtcgatc
     5461 ggcaaggagg tcgccggttc cggcgtgctc gtgaactgca tcgcgccggc cgtcatcgac
     5521 acaccgattc tcggtcagct gagcgaggag cacatcggct acatgctgca gaagatcccg
     5581 ctcgggaggg tcggcaagcc ggaagaggtt gcccacttga tcgcctatct cgccagcgag
     5641 aatctgagct tcgcgactgg ggcctgcttc gacatctccg gcgggcgagc ggtctattga
     5701 ttcggatcgt ccgcttcaga gagccgggca cggacgcgcg cctcgggctg gtggcaccgg
     5761 acgcgccgac gaccgtagcc gagctcgaga gcgacgacct gctcgccgtg ttgcgcggtg
     5821 cgcgcgctgt gagcggacgg acgaccgagg cgctcgacgc cgagacgctc gagctaccgc
     5881 cgccgtttca gctgcttgcc ccgctcgtgc cgcccgagac atgggccgcc ggcgtgacgt
     5941 acgagcggag ccgcgatgct cgcgtcgacg agagccaggt ggaggcgcgc gacgtctaca
     6001 acctcgtcta cgacgccgag cgtccagagt tgttcctgaa ggatgcggcc ggccgtcgca
     6061 ccgtcggccc cggggcggcg atcggcgtcc gctccgacgc ccagtggaca gtgccggaac
     6121 cggagctcgc actcgtcctt gactcggagg gcgagttgct cggggcaacg atcggcaacg
     6181 acgtgaccgc acgggacgta gaagccgcca accctctcta tcttcctcag gccaagctct
     6241 tcgcggccgc ctgcgcgctc ggcccggcga tcgttccccc tgaggattgg tcgcagccgt
     6301 tcgaaatcaa attgcggatc atcgggcccg ccggcgacgt cgtgtacgaa gcgcagacct
     6361 cgaccgccag aatgcgccgc acgttcgagg agcttgccgc gtgggtggtg cgcgagaatc
     6421 ccgtccctgc aggcagcgtt ctgctgaccg gtaccggtct cgtgccgccc gacgacgtga
     6481 cgcttgtgcc gggccagcaa gtcgagatcc ggatcccggg aatcggcacg ctcgtcaatc
     6541 cggtcggcgc ggcagccgac cttcttccct ccgaaaggag caagacccat gtccgatagc
     6601 gtcctggagg cgcccgcgcg gcccgagctc gccaactacg tcggcggcga atggacgccg
     6661 gcgcgcagcg gccgaacgta cgagaagcgc aatcccgcca acccggccga ggtcgtcggc
     6721 gagttccccg cgtccggcga ggaggacgta gccgcggcgg tcgaggctgc ggcgaaggca
     6781 ttccccggct ggtcgggact gccggctgcg gggcgcgccg cggtgctgat gcgcgcggcc
     6841 aacgcgatcg aagttcgcgt cgaggagatg gctgcggaca tgacgcgcga gatgggcaag
     6901 ccgttgcgcg aagcgcgcat ggagtcggcc cgcacggcgg cgatcttccg ctacttcgcc
     6961 ggcgaggcgg gacgcccgaa aggcgagctc tacgagcagt ccgcgaccgg ctcgaccctc
     7021 tacaccctac ggcggccgct cggcgtcgtc ggactgatca cgccctggaa cttcccggcg
     7081 gcgattcccg cctggaagat ggctcctgcg ctcgtctacg ggaacaccgt cgtgctgaag
     7141 ctggcgcagg aggcgccgct caccggactg caccttgccg ccgcgctgga cgaggccggc
     7201 attccggagg gagtgctga
//
LOCUS       DASVEU010000098         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_843261,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000098 DASVEU010000000
VERSION     DASVEU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843261"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF225_03760"
     CDS             <1..404
                     /locus_tag="VF225_03760"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144401.1"
                     /translation="GAKAAIEFYTNVLGATERMHMPGPDGKIGHAELELGDSLIMLAD
                     EFPEMGVLGPKAVGGTPVMIMIYVEDADAVFDAAVAAGATALRPMEDQFYGDRTGQFE
                     DPFGHRWSVATHVEDVPPDEMEKRAAEAMSG"
     gene            complement(451..1476)
                     /locus_tag="VF225_03765"
     CDS             complement(451..1476)
                     /locus_tag="VF225_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX7144402.1"
                     /translation="MTKKQEQKIVQYLNEAHGAELALVRQLQAQIAMTPKGQYRDGLD
                     THLQETRRHAERLEGRLAELGQGSNPLQVGLGALESLVGQALALTKTPFDLVRGTGGE
                     EKVLKNAKDSCAAEALEIATYTALARLARTAGDAKTERLADSVLRDEQRMLDRLLKEI
                     PRLTDAVARAEFDGAGSYDVTETGAADTLREVGGTAKRTTRKGAGQAKRTARQARKVP
                     GVARAEGTVKGAVASQSDLAIANYDSLSASEVQDKLSELSQVDLAKVDAYERKNQNRS
                     TITSRIDSLQASEPWPGYDELGVEEVRAVLSEGDDTRIKAVRDYERKHKNRAGVLQAT
                     ERELSNA"
     gene            complement(1602..1955)
                     /locus_tag="VF225_03770"
     CDS             complement(1602..1955)
                     /locus_tag="VF225_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144403.1"
                     /translation="MATTGATGSTRVDTPRTNGIRGRIAERNHETKAAIKTTEFWAML
                     GLVVTILVSAALINGGDNGTDEFIAKQAWLYVSILGGAYFISRGLAKSGSYEPDSDYN
                     PVDLAERKRNSDNDR"
     gene            complement(2169..2981)
                     /locus_tag="VF225_03775"
     CDS             complement(2169..2981)
                     /locus_tag="VF225_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX7144404.1"
                     /translation="MMCAVALAPPRLSQTTHPEGGDRVAARELERELLLRYHEFGDLA
                     AREELVERLLPLARDLALRYTYADEPFDDLLQVASVGLIKAIDRFEPGRGTKFTSYAA
                     PTILGELKRHFRDKGWSLHVPRDLQERSLAVSRATEVLSKELGRSPNVREVAGHIGCS
                     VEQVLEAQEAAASYEAASLDAPIARDDDESAALVDLLGSEDTAYELVEDREAIARTWK
                     ELPDEERSVLELRFMHDLNQREIGERIGYSQMHVSRLLRRALTRLETAATAA"
     gene            3207..3791
                     /locus_tag="VF225_03780"
     CDS             3207..3791
                     /locus_tag="VF225_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144405.1"
                     /translation="MPAVEALPQSKPDARERILDTAYELFSRRAIRDVGIDEVIAQAG
                     VAKATLYRHFPSKDELVIAFLERREERWTLAWVEAEARRRGTTPEEQLLAIFELFDEW
                     FHRDDFEACSFINVLLEMGPAHPAGQASVRHLASIRSVVGRLAEEAALRDPESFARSM
                     HILMKGSIVSAAEGDAEAAQRARSMAGLLIEQHR"
     gene            3924..4622
                     /locus_tag="VF225_03785"
     CDS             3924..4622
                     /locus_tag="VF225_03785"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX7144406.1"
                     /translation="MTETPHRDAKLVELLNEAYTKEKQLESALDAHIRVTTRDDYAKR
                     LKAHLKETKAHATQVSTRIKQLGGNPETVSLPGPEGLSRAAEGVSEMVGKAKAAAQGP
                     MHTVRGSGEQEKMLHNARVEYQQEAHEIATYTVIDSLATAAGDKQTAKLARDILRQEV
                     SMQKFLAGLLPKLSVDVAHDEIPVSEINGPAARVTAGGATGAKTSGGSKSGAGRSKTS
                     SRTSRSSSRRKAKA"
     gene            complement(4631..>5257)
                     /locus_tag="VF225_03790"
     CDS             complement(4631..>5257)
                     /locus_tag="VF225_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7144407.1"
                     /translation="ADPCRAPASRRRTGVLLGSSAALRGPSATLPVPWVETAVGALVD
                     AGIGPVEIHCLHVPNAANGWVKASTLQAVRAGLQLAPPMARVVCGDLNTPRRELEGGE
                     VVSFARDSRGRLRRERGLEWDEAELGVVPGLRDLGYQDAYRSLHGYGSREPSWTWQRI
                     SGHGGGWRLDHLFSSAELRPAACIYHHAWRDAGLSDHSALEADIVRMP"
BASE COUNT          818 a         1769 c         1807 g          863 t
ORIGIN      
        1 acggcgcgaa ggccgcgatc gagttctata cgaacgttct gggtgccacg gagcggatgc
       61 acatgccagg gcccgacggc aagatcggcc acgccgagct cgagctcggc gactcgctca
      121 tcatgcttgc tgacgagttc cccgagatgg gcgtactcgg gccgaaggcc gtgggcggca
      181 cgccggtgat gatcatgatc tacgtcgagg acgcggacgc agtcttcgat gccgccgtgg
      241 cagcgggcgc caccgccttg cgtccgatgg aggaccagtt ctacggggac cgcaccggtc
      301 aattcgagga ccccttcggg caccgctgga gcgtcgcgac ccacgtcgag gacgtgccgc
      361 ctgacgagat ggagaagcgg gcggcagagg ccatgagcgg ctgagggagg gggcccgcgc
      421 atggccccct cccttggctc gaaacggttc ttaggcgttg ctgagctccc gctcggtggc
      481 ctggagcacg ccggcgcggt tcttgtgctt gcgctcgtag tcgcgcacgg ccttgatgcg
      541 ggtgtcgtcg ccctcggaga gcacggcacg gacttcctcc acgcctagct cgtcgtagcc
      601 gggccagggc tcggacgcct gcagcgagtc gattcggctg gtgatggtgg agcggttctg
      661 gttcttgcgc tcgtaggcgt cgaccttggc cagatcgacc tgggagagct cggagagctt
      721 gtcctgaacc tcgctcgcgc tgagcgagtc gtagttcgcg atcgccaggt cggactggga
      781 cgccaccgcg cccttgaccg tgccctcggc gcgtgcgacg cccgggacct tgcgggcctg
      841 gcgggcagtg cgcttcgctt gtcccgcccc cttgcgagtg gtgcgcttgg ccgtgccgcc
      901 cacctcgcgc agcgtgtcgg cggcgcccgt ctcggtcacg tcataggagc cggctccgtc
      961 gaactcggcc cgagccacgg catcggtcaa tcgcgggatc tccttcagaa gccggtcgag
     1021 catccgctgc tcgtcccgga gaacggaatc ggccagacgc tcggtcttgg cgtctccggc
     1081 ggtgcgtgcg agccgggcca gggcggtgta ggtggcgatc tcgagcgcct cggccgcgca
     1141 cgagtccttg gcgttcttga gcaccttctc ctcgccaccg gttccgcgaa ccagatcgaa
     1201 cggggtcttg gtcaacgcca gggcctgccc aacgaggctc tccagggcac cgagtccgac
     1261 ctgaagcggg ttgctcccct gccccagctc ggccagccgg ccctcgagac gctcggcgtg
     1321 ccgccgcgtc tcctggaggt gggtatccag cccgtcgcgg tactgaccct tgggggtcat
     1381 cgcgatctgc gcttggagtt ggcgcacgag cgccagctcc gcaccatggg cctcgttcaa
     1441 gtactgcacg atcttctgct cttgcttctt agtcacgaaa attcactctc agtggggggt
     1501 agttgccagc gggcccgcgc aggctgcgag tggccggcgt aggtcgagga ggcagagcgc
     1561 cgggcggctc gaggccgccc ggcttgctcc actaggtcgg tctagcgatc gttgtcgctg
     1621 ttgcgcttac gctcggcgag atcaaccggg ttgtagtcgg agtcgggctc gtagctgccc
     1681 gacttcgcga ggccccggga gatgaagtag gccccgccca gaatcgagac gtagagccaa
     1741 gcctgcttgg cgatgaactc gtccgtcccg ttgtcgccgc cgttgatcaa tgcagcgctg
     1801 acgagaatcg tcacgacgag tccgagcatg gcccagaact ccgtggtctt gatggctgcc
     1861 ttggtctcgt ggttgcgctc cgcgatccgg ccgcggattc cgttcgtccg cggcgtgtcg
     1921 acgcgggtgc tgccagtggc accagtggtg gccatgtgtg gctccttatt gaggttgatt
     1981 ggtgtctcga agccccaagg gcgagcgcgt ctggcgcccg ctccgtcagt gcttcttgtc
     2041 gagtcctcgg gggcggtcgt cgccttggct ccggtaccgg ccccgcccca ctcctgtgcc
     2101 cgtggcaggg cgacgcaaac aggctcagcg ctaccccggt cgggctcgag tcaggacggt
     2161 gtcagtggtt acgctgccgt cgcggctgtc tcgagacgcg tcagcgcccg ccgaaggagc
     2221 cgcgagacgt gcatttgcga gtagccgatc cgctcgccga tctcgcgctg gttgaggtcg
     2281 tgcatgaagc gcagttcgag gaccgagcgc tcctcgtccg gcaactcctt ccaggtccgt
     2341 gcgatcgcct cgcggtcctc gacgagctcg taggccgtgt cctcgcttcc gagcaggtcc
     2401 acgagcgccg ccgactcgtc gtcgtcacgt gcgatcggcg cgtcgagtga cgcggcctcg
     2461 tagctcgccg cggcctcctg cgcctccagc acctgctcga cggagcagcc gatgtggccc
     2521 gccacctcgc gcacattagg cgagcggccg agctccttcg atagcacctc cgtcgcgcgg
     2581 ctcaccgcga gcgagcgctc ctggaggtcg cgcggcacgt gcagggacca gcccttgtcg
     2641 cggaagtggc gcttcagctc gccgaggatg gtgggcgcgg cgtagctcgt gaacttcgtg
     2701 ccgcgccccg gctcgaagcg gtcgattgcc ttgatcaggc cgacgctcgc gacctggagc
     2761 agatcgtcga agggctcgtc ggcgtacgtg tagcgcagcg ccaggtcgcg cgccagcggc
     2821 aggaggcgct caacgagctc ctcgcgtgcg gcgaggtctc cgaactcgtg gtagcggagc
     2881 aggagctctc gctctagctc ccgcgcggca acgcggtcgc cgccttcggg gtgagtggtc
     2941 tgggacagcc tcggcggtgc gagggcgact gcacacatca tgaacctccg tcggtttctg
     3001 ttgacgcaag gtccgagtcg cgacccattc gaccgggtgc cgcgcgacgt cccttcaaaa
     3061 cccgaggacg tcgcttcgag cggctctgtg tgcccatcgt cgcgctttcc cagcagcctc
     3121 atgctgctct ccgacaccac tactgccgga gcgaactgcg ttttatagca aagacagacc
     3181 ggtctgtcta gtgttaagct ccaggcatgc ccgcggtgga agccctcccg cagtcgaagc
     3241 cggatgcgcg tgagcgcatc ctcgacaccg cctacgaact cttctcccgg cgtgcgatcc
     3301 gcgacgtggg catagacgag gtgatcgcgc aagccggagt ggcgaaggca accctgtatc
     3361 gccacttccc gtccaaggac gagcttgtga tcgcgttcct cgagcgccgc gaggagcgct
     3421 ggacgctggc gtgggtcgag gcagaggcga ggcgacgcgg caccacaccg gaggagcagc
     3481 tactcgcgat cttcgaactg ttcgacgaat ggttccaccg cgacgacttc gaagcctgct
     3541 cattcatcaa cgtgctgctc gagatgggcc ccgcgcaccc cgccggtcag gcgagcgtcc
     3601 ggcatctcgc gagcatccgc tcggtcgtcg gtcgtctcgc agaggaggcc gcactgcgcg
     3661 acccagagtc cttcgcgcgc tcgatgcaca ttctcatgaa gggctcgatc gtctccgccg
     3721 cggaggggga cgccgaggcg gcacagcgag cccgatcaat ggcaggcctc ctgatcgagc
     3781 aacaccgcta gtcgccgcgt cgctgtaccc gtcgaaggcc gcatgaaccc atttgcgggg
     3841 gttttcccaa gacggtcgtt cgaccggcca ttttcgggta gcagcctcgc gaacggattc
     3901 gaccgagagg aaggaagacc gccatgaccg agacccccca ccgcgatgcg aagctcgtcg
     3961 agctcctcaa cgaggcgtac acgaaagaga agcagctcga gtctgcgctc gacgcgcaca
     4021 tccgggtgac gacccgcgac gactacgcga agcgtctaaa ggctcacctc aaggagacca
     4081 aggcgcacgc gacccaggtg tcgacgcgaa tcaagcagct tggcggcaac ccggagactg
     4141 tctcactgcc cggccccgag ggcctgagcc gcgccgccga gggcgtctcc gagatggtcg
     4201 gaaaggcgaa ggccgcggcc caggggccga tgcacacggt gcgcggatcg ggtgagcagg
     4261 agaagatgct tcacaacgct cgcgtcgagt accagcagga ggcgcacgag atcgcgacct
     4321 acaccgtgat cgactcgctc gccaccgcgg ccggcgacaa gcagacggcc aagctcgcgc
     4381 gtgacatcct gcgccaggag gtgagcatgc agaagttcct cgccgggctg ctgccgaagc
     4441 tgtccgtgga cgtggctcac gacgagattc ctgtctcaga gatcaacggg ccggcggccc
     4501 gcgtgacggc tgggggcgca acgggcgcga aaacctccgg gggctcgaag tccggcgccg
     4561 gccgaagcaa gacgtcgagt cgaaccagcc gatcgtcgag ccgacgcaag gccaaggcct
     4621 gacgggccgc tcatggcatt cggacgatgt cggcctcgag cgccgagtgg tcgctcaggc
     4681 ccgcatcgcg ccaggcgtgg tggtagatac aggccgctgg ccgcaactcg gccgagctga
     4741 acaggtgatc gaggcgccag ccgcctccat gccccgagat gcgctgccac gtccagctcg
     4801 gctcgcgcga tccgtagccg tgaagggagc gatacgcgtc ctggtagccg aggtctcgca
     4861 accctggaac gacgccaagc tcggcctcgt cccactcgag tccgcgttcg cgccgcagtc
     4921 gcccgcgtga gtctctcgcg aaggagacca cctcgccgcc ctcgagctcg cggcgcgggg
     4981 tgttgaggtc gccgcacaca acgcgggcca tcggcggcgc gagctggagc ccggcgcgaa
     5041 cagcctggag cgtgctcgcc ttcacccagc cgttggccgc gttggggacg tggaggcagt
     5101 ggatctcgac tggaccgatg ccggcgtcga caagcgcgcc taccgcggtc tcgacccacg
     5161 gaacgggtag cgtcgcggac gggccgcgaa gggcggcgct cgatcccagc agcacgccgg
     5221 tgcgacgccg cgacgccggc gcgcggcagg ggtcggc
//
LOCUS       DASVEU010000099        10716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1233439,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000099 DASVEU010000000
VERSION     DASVEU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233439"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="VF225_03795"
     CDS             complement(<1..1997)
                     /locus_tag="VF225_03795"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7144408.1"
                     /translation="MAVLEAHEKHQELIDELIAAVAAYNPEVDRDLIRRAFLFAANAH
                     EGQQRRSGEDFVLHPWGAAKICAQLRLDDETIAAALLHDVVEDTDAELEDVRAEFGDE
                     VAKLVEGVTKLTRVQFQSREQAEAENYRKLIVAMAADVRVILIKLADRLHNLREIEYL
                     GKQKQIQKAKETLEVYAPLAHRLGIHAMKWELEDLAFETLHPRKYEEIKAMVSERRAD
                     RESHVSEAARVLQLELTKVDIPVEISGRAKHFYSIYDKMAKKGREFNEIYDLTAMRVT
                     VERSGDEGTRDCYGALGLIHSLWRPMPGRFKDYIAMPKFNRYRSLHTTVIGPEGRPLE
                     IQVRTREMHETAEFGIAAHWRYKGGRHPRTDEAWESWVRQLMEAQPETIDEPGEYKRA
                     FLTDLVDDEVYVFTPKGEVKTLPSGATPIDFAYAVHTDVGHRTVGAKINGRIVPLHYR
                     LKNGDFVEILTSKAGRGPSRDWISLAASSRARNKIRQWFSRETREDQEQKGRDSLEQS
                     LKAAKLPYRKLVGSAVLAEVIRDMNFKKAEDFYLALGSGKIGPQQVVNKVLQRLKTEQ
                     VAEEEAVPLKAPRAKHAVDSDNYGINVHGVDEVLVRLAKCCTPVPGDEIVGYISLGKG
                     ITIHRGDCPNVKALARQPERFTPVSWDGGATQSFRVQIAVES"
     gene            complement(2276..4147)
                     /gene="recJ"
                     /locus_tag="VF225_03800"
     CDS             complement(2276..4147)
                     /gene="recJ"
                     /locus_tag="VF225_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7144409.1"
                     /translation="MSEGIWTIRPCPHRQASGLAKELGLNEITASVLVRRGYGDPEVA
                     RAFLAGEQPLHDPFLLGDMAGAVEQIRAAIAAGKRICVHGDYDVDGISATVLAILVLR
                     ELGAEVEWHLPSRFDEGYGVSGQTLERLAAEGCGLVLTVDCGITAVDEVRRAHELGLE
                     VIVTDHHRPGEELPDCPIVATRPSDYPFQELCGTAVVYKLGQALLGAESEVLRRHLDL
                     VALATIADVVPLVGENRSLAIAGLRTLARTQKLGLRALMKAAHVDPAAVDAGKVGFRL
                     APRINAAGRLGDPSAALELLLTEDVDEARRLADRLEELNRDRQAVEDKIVRAAIAQVE
                     EWPEAKRRRSAYVVWGEDWHEGVIGIVASRLVERYHRPVVLIAGTDGLWKGSGRSIPS
                     FDLHGALGACSTFLERFGGHRAAAGLSIAPERVEPFAEAFAAQAEGLLGPEDLTPATV
                     VDAVLPRGAKLTLELCEELRALAPFGLANPDVTLLAPGCELGELATVGDGKHLRFRVQ
                     LDGRDAGGAIAFGQGTKLDSYRREGRYDVAFRLQENHWNGTVSPQLVVRRVFDADHRF
                     DEVYAWLRGQWQARPRDAQAQTIFDELEVEEGGPKRHPLESETFRSLLAEPALVRAA"
     gene            4168..5301
                     /locus_tag="VF225_03805"
     CDS             4168..5301
                     /locus_tag="VF225_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7144410.1"
                     /translation="MSDGARLREKFLLDPSVTFLNHGSFGATPRAVFERYQEWQLELE
                     RQPVLFLDRRLDALLAEARAVLGAYVGADPDDLVFVPNATAGVNVAAWPLGLQPGDEV
                     LSTDLEYGALDLTWEHVCGDFGARYVRTPIRLPVTSAEEIVDAVWAGVGPRTRALFLS
                     HHTSATAMTLPVEEICRRAREAGIQTIVDGAHVPGHLPLNLRELDADYYAANCHKWLG
                     APKGAGFLYVRRELQRDVHPLLISWGYGGDDPSFVSRHEQQGTRDPSAYLTVPAAIAW
                     QDEHEWPAVRDRCHELARRARNELDLEPLTPDSNEFFAQMVTLRLPKSAPRDLQERLY
                     AEHRIEIPVFERGDERFIRASFQGYNDTADLERLKAALFGLLG"
     gene            complement(5419..5610)
                     /locus_tag="VF225_03810"
     CDS             complement(5419..5610)
                     /locus_tag="VF225_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144411.1"
                     /translation="MTDTTLTELHHRSADGIEVSLLWSRITNALTVAVEDSRSGLSFE
                     VPAPAEKALDVFEHPYAYA"
     gene            complement(5712..5990)
                     /locus_tag="VF225_03815"
     CDS             complement(5712..5990)
                     /locus_tag="VF225_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144412.1"
                     /translation="MSARLAAASVFVTALVVVTVWLTDLSFGRAALLAPVLVIGLAAV
                     AGLVVFWGRVGWDSLRRARHPRLLVAAAVAFILLLVGLTLLGVQLPRE"
     gene            6024..6479
                     /locus_tag="VF225_03820"
     CDS             6024..6479
                     /locus_tag="VF225_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144413.1"
                     /translation="MTEANYVSGDDYVVEFLGYRFGFNCSDFEQRVTAAAVKLGLVGD
                     NELDDDETADLVELVERDWIDEPRSGLGRYLVRHWERVSLVGGESLVYWLKKLVFRGA
                     WLDHRVKEGLLEVAWDEDVADFGYRDPNGDRALLELAPVPSWHELQFRR"
     gene            6452..7180
                     /locus_tag="VF225_03825"
     CDS             6452..7180
                     /locus_tag="VF225_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144414.1"
                     /translation="MARAAVQALIRAAAIAAFLAALLAVDTQVELPGQLALGALTWIV
                     LLVAARPLPAERRAQVAVVICAATVAEVTGSILWGVYSYRLHNLPTFVPPAHGLVFMA
                     GLSLSEALRRHSRALVAFAAAAVTIWGVLGLTVLPRLDAAGALCIPLLLAFLWRSPAR
                     AAYAGVFLVVAALELYGTAIGTWRWAAELPGLGIPDGNPPSGVAAGYVWFDVMALLLA
                     PRLLAAVQRTWHLRRRLVPGTGAA"
     gene            complement(7517..7858)
                     /locus_tag="VF225_03830"
     CDS             complement(7517..7858)
                     /locus_tag="VF225_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144415.1"
                     /translation="MSPDLWVPGAAEPSLDGFVYRVHRQIERYTATHSADLTHVEVEL
                     GDGERLTLRSLSAEPGYGFLTLSVDAEKGDSEEHIVPIGAIRRIALGPADESRRPGFA
                     LSKEIGPEPAA"
     gene            complement(7855..8871)
                     /gene="ruvB"
                     /locus_tag="VF225_03835"
     CDS             complement(7855..8871)
                     /gene="ruvB"
                     /locus_tag="VF225_03835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7144416.1"
                     /translation="MTSAPQFLVPAVMPEEDDLEQSLRPRRLAEFVGQERIKEQLAIS
                     LDAAKGRDEALDHVLLVGPPGLGKTSLAFIVREELGVGIRCVAGPALERKGDMAAILT
                     SLERHDVLFVDEIHRLNRAIEEILYPALEDFRLDIIVGQGAAARTLTLDLPPFTLIGA
                     TTRTGLLTTPLRDRFGMTFRLEYYEPKELGTIVRRSARILDVEIAEDAADEIARRSRG
                     TPRIANRILRRVRDVAQVKHDGKITNQVAREALELLEVDGDGLERIDRALMETILVKF
                     GGGPVGLSTLAVALGEEQDTIEDVYEPYLLQLGFLQRTPRGRIITELGRERFGARGED
                     TKLF"
     gene            complement(8872..9438)
                     /gene="ruvA"
                     /locus_tag="VF225_03840"
     CDS             complement(8872..9438)
                     /gene="ruvA"
                     /locus_tag="VF225_03840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7144417.1"
                     /translation="MIARLRGRPLANTPDGLVLDVGGVGYLVAATPSAVRAAEGAAEV
                     SLHTYLHVREDALQLYGFAEAAERELFLQLLSVNGIGPKVALAIVSGSPAADLRRAIV
                     LGDTARFQAIPGIGKKTAERILLELKEKMGDAPVPISSAGGTQPHLVARDALVELGYS
                     LGEAEMALARVDPELPPEERVREALKAA"
     gene            complement(9435..9926)
                     /gene="ruvC"
                     /locus_tag="VF225_03845"
     CDS             complement(9435..9926)
                     /gene="ruvC"
                     /locus_tag="VF225_03845"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7144418.1"
                     /translation="MKVIGIDPGTASCGYGIVHEKDGRLRAIDHGWWKTSAAERPELR
                     LKTIFDAVVELVALHQPDAVAIEESFVGVDARTALSVGQARGAIMVAAANAGVGCAEY
                     SPARVKQVVCGYGRADKQQMQRMVKVVLGLQDEPATTHAADALAVAICHALAPPLLRI
                     AAG"
     gene            9980..10573
                     /locus_tag="VF225_03850"
     CDS             9980..10573
                     /locus_tag="VF225_03850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7144419.1"
                     /translation="MPGDTWLTFPDGTEHELKESVTIGRDASSDLAFESPTVSREHAS
                     LTFRDGRWYVEDRGSFNGTFLNGTRVQPGTPLPLRHADRIGIGSETVLFSWPAQLEDP
                     DTTEPLEEIAPTASAQLSAFQRQVVQGLCGPWLAGASLESLPSNEQIAAQLGTPGATG
                     TVKAALRRIYAKAGLSDQPAHAKRRALCRVARQRGWV"
BASE COUNT         1671 a         3699 c         3570 g         1776 t
ORIGIN      
        1 gattcgacgg cgatctggac gcggaagctc tgcgttgcgc cgccgtccca gctcacgggt
       61 gtgaagcgct cgggctggcg ggcaagcgcc ttcacgttcg gacagtcgcc cctgtggatc
      121 gtgatgcctt tgccgaggga gatgtaaccg acgatctcgt ctcccggcac cggcgtgcag
      181 cactttgcga gccgcacgag cacctcgtcg acgccgtgga cgttgatccc gtagttgtcg
      241 ctgtcgacgg catgcttcgc gcgcggcgcc ttgagcggga ccgcttcctc ctcggccacc
      301 tgctccgtct tcagccgctg caggaccttg ttgaccacct gctgcgggcc gatcttgccg
      361 gagccgagcg cgaggtagaa gtcctcggcc ttcttgaagt tcatgtcgcg gatgacctcc
      421 gcgagcacgg ccgacccgac gagcttgcgg taaggaagct ttgccgcctt cagcgattgc
      481 tccagcgagt cgcggccctt ctgctcctga tcctcgcgcg tctcgcgaga aaaccactgc
      541 cggatcttgt tacgcgcgcg cgaggaggcc gccagcgaga tccagtctcg cgacgggccg
      601 cggccggcct tcgaggtcag gatctcgacg aagtcgccgt tcttcagcct gtagtgcagc
      661 gggacgatgc ggccgttgat cttggcgccg accgtgcgat gcccgacgtc cgtatgcacg
      721 gcatacgcga agtcgatcgg agtcgcgccc gaaggcaggg tcttcacctc gcccttcggc
      781 gtgaagacgt agacctcgtc gtcgacgagg tccgtcagga acgcgcgctt gtactcgccc
      841 ggctcgtcga tcgtctcggg ctgcgcctcc atcaactggc gcacccagct ctcccaggcc
      901 tcgtcggtcc tcgggtggcg cccgcccttg tagcgccagt gagcagcgat cccgaactcg
      961 gccgtctcgt gcatctcgcg cgtgcggacc tgaatctcga gcggacgtcc ctccggaccg
     1021 atcacggtcg tgtgcagcga tcggtagcgg ttgaacttcg gcatggcgat gtagtccttg
     1081 aagcgaccgg gcatcggcct ccacaaggaa tggatcaggc cgagcgcgcc atagcaatca
     1141 cgagtacctt cgtcgcccga gcgctccacg gtcacacgca tcgccgtgag gtcgtagatc
     1201 tcgttgaact cgcggccctt cttggccatc ttgtcgtaga tggagtagaa gtgcttcgcg
     1261 cgacccgaga tctccacggg gatgtcgacc ttcgtcagct cgagttgcaa gacgcgcgcc
     1321 gcctccgaga cgtggctctc ccgatcggca cggcgctcgc tgaccatcgc cttgatctcc
     1381 tcgtacttac ggggatgcag agtctcgaac gccaggtcct cgagctccca cttcatcgcg
     1441 tggatgccga gccggtgcgc gagcggcgcg tagacctcca gggtctcctt cgccttctgg
     1501 atctgcttct gcttcccgag gtattcgatc tcgcggaggt tgtgcagccg gtcggccagc
     1561 ttgatcagga tcacgcgcac gtctgcggcc atcgcgacga tcagcttgcg atagttctcg
     1621 gcctcggcct gctcgcggct ctggaactgg acgcgcgtca gcttcgtcac gccctctacg
     1681 agcttggcga cctcgtcgcc gaactcggca cggacgtcct cgagctcggc gtcggtgtcc
     1741 tcgacgacgt cgtggaggag cgccgcggcg atggtctcgt cgtcaaggcg gagctgggcg
     1801 cagatcttgg ccgcacccca gggatggagc acgaagtcct cccccgaacg gcgctgctgg
     1861 ccctcgtggg cgttcgcagc gaagaggaag gcccgccgga tcaggtcgcg gtcgacctct
     1921 gggttgtagg cggccacggc cgcaatcagc tcgtcgatga gctcctgatg cttttcgtga
     1981 gcctcgagga ccgccatatc gtcagtgtac tccgggggta tcgggctccc ttcccccgtt
     2041 caaggggcgt atctcaaggg caggagccgg cgatccgatg cagaagggga gtcgtccatg
     2101 cactggttcc gacgccgaaa agaacgccgt gccgccgtgc gcgcagcacg actcctcctt
     2161 gcgctcgatg cgctcgcccc gccccgcgcc ccgcgtcagc cgcgcgtccg ccgggcgtcc
     2221 gtcggcatcg cccgctaacc gacgttccgg aacgccagcc gaaggcgagc gacgctcacg
     2281 ccgcgcggac gagtgcgggc tcggccagca gcgagcggaa cgtctcggac tcgagcggat
     2341 ggcgcttcgg ccctccctcc tccacctcga gctcgtcgaa gatcgtctgt gcctgtgcgt
     2401 cgcgtggccg ggcctgccac tgcccgcgca accacgcgta gacctcgtcg aagcgatggt
     2461 cggcgtcgaa gacgcgacgg acgacgagct gcggcgacac cgttccgttc cagtgattct
     2521 cctgtaggcg gaacgcgacg tcatagcgtc cctcgcgccg gtagctgtcg agcttggttc
     2581 cctgcccgaa cgcgatcgcc ccgccggcat cgcggccgtc gagctgcaca cggaaacgga
     2641 gatgcttgcc gtcgccgacc gtcgcaagct cgccaagctc gcaccccggc gccaggaggg
     2701 tcacgtcggg attggccagg ccgaacggcg ccaacgcgcg cagctcttcg cagagctcga
     2761 gcgtaagctt cgccccgcgc ggaaggactg cgtcgacgac cgtcgccggc gtgagatcct
     2821 cggggcccag caggccttcc gcttgagccg cgaacgcctc ggcgaacggt tcgacccgct
     2881 ccggcgcgat cgacagaccc gccgctgcgc gatgaccgcc gaaccgctcg aggaaagtcg
     2941 agcatgcccc gagcgcgccg tggagatcga acgacgggat cgaacgcccg gatcctttcc
     3001 agaggccgtc cgtccccgcg atgaggacga ccggccggtg ataacgctcg acgagacgtg
     3061 acgcgacgat gccgatcacg ccttcgtgcc agtcttcgcc ccagacgacg taggcagaac
     3121 ggcgccgctt cgcttccggc cactcctcga cctgcgcgat cgcggcgcgc acgatcttgt
     3181 cctccactgc ctgccggtcg cgattcagct cctcgagtcg atcggcgaga cgccgcgcct
     3241 cgtccacgtc ctccgtcagg agcagctcga gcgctgcgct cggatcgccc agccggcccg
     3301 ctgcgttgat ccgcggcgca agccgaaagc cgaccttgcc cgcgtcgacg gcggccggat
     3361 ccacgtgcgc ggccttcatc aatgcacgca aaccgagttt ctgcgtgcgc gcgagcgtgc
     3421 gcagccccgc gatcgcaagc gatcggttct cgcccacgag cggaacgacg tccgcgatcg
     3481 tcgcgagcgc gacgagatcg agatgccgac gcagcacttc ggactcggca cccagcagcg
     3541 cctgcccgag cttgtagacg acggcggtgc cgcagagctc ctggaagggg taatcggaag
     3601 ggcgggtcgc gacgatcgga cagtccggca gctcctcgcc tgggcggtgg tggtcggtga
     3661 cgatgacttc gaggccgagc tcgtgcgcac gccgaacctc atctacggcg gtgatcccgc
     3721 agtcgacggt caggaccagt ccgcaaccct ccgcagcgag gcgctcgagc gtctggccgc
     3781 tgaccccgta gccctcgtcg aagcggctcg gcaaatgcca ttcgacctct gcgcccagct
     3841 cccgaaggac gaggatggcg agcactgtgg cgctgatccc gtccacatcg tagtcgccgt
     3901 gtacgcagat ccgtttgccg gccgcgatcg ccgcgcgaat ctgctcgacg gcaccggcca
     3961 tgtcaccgag caggaaggga tcgtgcaggg gctgttcgcc ggccaggaag gcccgcgcca
     4021 cctcggggtc gccgtagccg cgccgaacga gcacgcttgc cgtgatctcg ttgaggccga
     4081 gctccttcgc gaggccgctc gcctgccggt gcgggcacgg acggatcgtc cagatgccct
     4141 ctgacatgcc cgactaccgt acgagcggtg tcggacggcg cccgcctgcg ggagaagttc
     4201 cttctcgatc caagtgtgac gtttctgaac cacggctcct ttggggcgac gccgcgagca
     4261 gtgttcgagc gttaccagga gtggcagctc gagctcgagc ggcagccggt gctgtttctc
     4321 gaccgccggc tggacgcgct gctcgcagag gcccgtgcgg tgctcggtgc ttacgtcggc
     4381 gccgatccgg acgacctggt gtttgtcccg aacgcaacgg ccggcgtgaa cgtcgccgcc
     4441 tggcccctcg gcctgcagcc gggcgacgag gtgctctcga ccgatctcga gtacggcgct
     4501 ctcgacctca cctgggagca cgtctgcgga gacttcggcg cgcggtatgt gcggacaccg
     4561 attcggcttc cggtgacgag tgcggaagag attgtcgatg cagtctgggc cggagtcggc
     4621 ccgcggaccc gcgcgctctt cctcagccac cacacgtccg cgacggccat gacgcttccg
     4681 gtcgaagaga tctgcaggcg cgcccgagag gcggggatcc aaaccatcgt cgacggcgcg
     4741 cacgtaccgg gacacctccc actgaacctg cgtgagctcg acgccgacta ttacgcggcc
     4801 aactgccaca agtggctggg cgcgcccaag ggcgcaggct tcctttacgt gcgccgcgag
     4861 ctccagcgcg atgttcatcc actgctcatc agctggggct acgggggaga tgacccgagc
     4921 ttcgtgtcac gccacgagca gcaggggacg cgcgacccgt ctgcctacct gacggtgccg
     4981 gcggcgatcg cgtggcagga cgagcacgag tggcctgcgg tacgcgaccg ctgtcacgag
     5041 ctcgcccgac gggcacgcaa cgagctcgac ctcgagccgc ttacaccaga ctcgaacgag
     5101 ttcttcgcgc agatggtcac gctccgtctg ccgaagagcg cgccgaggga tctccaagag
     5161 cggctctacg ccgagcatcg aatcgagatc ccggtgttcg aacgcggcga cgagcggttc
     5221 atccgtgcgt ccttccaggg ctacaacgac accgccgatc tcgagcgact caaggccgct
     5281 ctcttcggac tgctcggtta atcggtgagg acacctcgcc gccgcgccgg gcttttgccc
     5341 ggtgcggccg cgtacgctct ccgccagcgc ggtcgcattc gagtcatcat ggcgccgaag
     5401 gcgccggact cgaatgcgtt aggcgtacgc gtacggatgc tcgaagacgt cgagggcctt
     5461 ctccgcggga gccggaacct cgaagctgag gccggagcgg ctgtcctcga ccgcaacggt
     5521 cagcgcgttt gtgatgcggc tccacaggag cgagacctcg atgccgtccg ccgagcggtg
     5581 atgaagctct gtgagtgtcg tatcggtcat gtcctcaccg tcgcagttgg agagagctcc
     5641 cgacaccggg gacaccggtg tctttgggta cggtcaaccc ccgtcctaac cgggggctaa
     5701 tccctacgaa gtcactctcg gggaagctgg acgcccagga gcgtcaggcc caccagcagg
     5761 aggatgaacg cgaccgccgc ggccactagc aagcggggat ggcgcgcgcg gcgcagcgag
     5821 tcccagccga cgcggcccca aaagacgacg aggccggcga ccgccgcgag gccgatcacg
     5881 agcacgggcg cgagcagcgc cgcgcgtccg aaggagaggt cggtgagcca gaccgtgacc
     5941 accacgagtg ccgtcacgaa gacggatgca gcggcgaggc gagcgctcat gaggcaaacc
     6001 tagcgtggcg ctatgctcgg ctcgtgacag aggcgaacta cgtttcgggg gacgattacg
     6061 tcgtcgagtt cctcggctac cgcttcgggt tcaactgctc cgacttcgag cagcgcgtca
     6121 cggccgccgc ggtgaagctc gggctcgtgg gcgacaacga gctcgatgac gacgagaccg
     6181 ccgatctcgt cgagctcgtc gagagggact ggatcgacga gcctcgcagt gggctcggca
     6241 ggtacctcgt ccgtcactgg gagcgcgttt cgctcgtcgg cggcgagtcg ctcgtctact
     6301 ggctgaagaa gctcgtcttc cgcggggcgt ggctcgacca tcgcgtcaag gagggactgc
     6361 tcgaggtcgc ctgggacgag gacgtggcgg acttcgggta tcgcgacccg aacggcgacc
     6421 gcgcgttgct cgagcttgcg cccgtgcctt cttggcacga gctgcagttc aggcgttgat
     6481 ccgcgcagca gctatcgccg cattcctcgc ggcactgttg gcggtggata cgcaggtcga
     6541 gttgcccggc cagctcgcac tgggagcctt gacgtggatc gtgctcctgg tcgctgcgcg
     6601 gccgcttccg gcagagcggc gcgcgcaagt cgcggtggtc atctgcgcgg cgacggtggc
     6661 ggaggtgacg ggctcgatcc tgtggggtgt ctactcgtat cggctgcata acctgccgac
     6721 gttcgtcccc ccggctcacg gactggtgtt catggcgggt ctgtcgttga gcgaagcgct
     6781 ccggcgtcat tcacgcgcgc tcgtcgcctt cgcggcggcc gccgtcacga tctggggcgt
     6841 tctcggactg acggtgctgc cacgtctcga tgccgcgggt gcgctctgca tccctctgct
     6901 gctcgccttc ctctggcgct cgcccgcgcg cgccgcgtac gcgggggtct tcctcgtcgt
     6961 cgccgcgctc gagctctacg gaactgcgat cggcacctgg cgctgggcgg cggagctacc
     7021 gggcctaggc attcccgacg gcaacccacc gagcggcgtc gccgcaggct acgtctggtt
     7081 cgacgtcatg gcgctcctgt tggcgcctcg cctcctcgcc gcggtgcagc ggacctggca
     7141 cttacggcgt cgtctcgtgc ctggcactgg ggccgcgtag cggccagtcc gcgaagcgga
     7201 ccacgccgct acgggatcgg gccaggcgta gatagcggcg gccgcaggac aaacctgcga
     7261 ccgcccgttc ggaggatgtg agctgcggcc cctcacgatc ctcctgagac tcagaatcgg
     7321 ggtgaaaagg cgtttctgca ctcccccggc agggtgaaag tgaagcacct gaatgaacca
     7381 accaaagggg gaggaatcaa ccaggctggg tgatccatgc tcaacgtgta ccacctgtcc
     7441 tcaccggttc tgttctgcgg cagaaccagc cccgacaaca ctgcggccgg ggcggacatt
     7501 tcaacagggc cgggccctat gcggcgggtt ccggccctat ctctttcgac agggcgaatc
     7561 ccgggcggcg ggattcgtcc gctggtccaa gtgcgatccg cctgatcgcg ccgatcggga
     7621 cgatgtgctc ctccgaatcg cccttctcgg catcgaccga cagcgtcaga aacccgtagc
     7681 ccggctcggc ggacagcgac cggagcgtga ggcgctctcc gtcccctagc tccacctcca
     7741 cgtgggtcaa gtccgcgcta tgggtcgcgg tgtagcgctc gatctgacgg tggacgcgat
     7801 acacgaagcc gtcgagcgac ggctcggctg cccccggcac ccagagatcg gggctcaaaa
     7861 gagcttcgtg tcttccccgc gggctccgaa ccgctcgcgg ccgagctcgg tgatgatgcg
     7921 gccgcgcggt gtgcgctgta agaagcccag ctggagaaga taaggctcgt agacgtcctc
     7981 gatcgtgtcc tgctcctccc cgagggcgac ggcgagcgtg gacaggccta ccggtccgcc
     8041 gccgaacttg acgaggatcg tctccatcag cgcgcggtcg atgcgctcga gcccgtcgcc
     8101 gtcgacctcg agcagctcga gggcctcgcg cgccacctgg ttcgtgatct tgccgtcgtg
     8161 cttgacctgc gcgacgtcgc ggacccgtcg aaggatccga ttcgcgatgc gcggcgttcc
     8221 gcgggagcgg cgcgcgatct cgtctgctgc gtcctccgca atctcgacgt cgaggatccg
     8281 cgccgaccgc cggacgatcg tcccgagctc cttcggctcg tagtactcga gccgaaaagt
     8341 catgccgaag cggtcgcgca gcggcgtcgt caagagccct gttctcgtcg tcgcaccgat
     8401 gagcgtgaag ggcgggagat cgagggtcag cgtgcgcgcc gccgcgccct ggccgacgat
     8461 gatgtcgagc cggaagtcct cgagcgccgg atagaggatc tcctcgatcg cgcggttgag
     8521 gcggtggatc tcgtccacga acaagacgtc gtggcgctcg agactggtca ggatcgccgc
     8581 catgtcgccc ttgcgctcga gcgccgggcc cgcgacgcag cgaatgccga cgccgagctc
     8641 ctcgcggacg atgaatgcaa ggctcgtctt gccgagcccg ggcgggccga cgaggagcac
     8701 gtggtcgagc gcttcgtctc gccccttcgc tgcatcgagc gagatcgcaa gctgctcctt
     8761 gatccgctcc tgtccgacga actcggccag acggcgcgga cgcagcgact gctcgaggtc
     8821 gtcctcctcg ggcatgacgg cgggaaccag gaactggggc gcagaggtca tctacgccgc
     8881 ctttagcgct tcgcggacgc gttcctccgg cggcagctcc ggatcgaccc tcgcgagagc
     8941 catttcagcc tccccgaggg agtagccgag ctcgacgaga gcgtctctcg ccaccaggtg
     9001 cggttgcgtg cctcccgccg acgagatcgg caccggagcg tcccccatct tctccttcag
     9061 ctcgagcagg atgcgctcgg cggtcttctt gccgatccca gggattgcct ggaagcgcgc
     9121 cgtgtctccg agcacgatcg cccgcctcag atcggcggcc ggcgagccgg acacgatcgc
     9181 cagagcgacc ttcgggccga tcccgttgac ggacagcagc tggaggaaga gctcgcgctc
     9241 ggccgcctcc gcgaacccgt acagctgcag cgcgtcttcg cgcacgtgca gatatgtatg
     9301 cagcgacacc tccgccgcgc cctcggcggc tcgaaccgcg gacggcgtcg cggcgacgag
     9361 gtacccgaca ccgccgacgt cgagaacgag cccgtccggc gtgttcgcca ggggacggcc
     9421 ccggaggcgc gcgatcatcc cgccgcgatc ctcagcagcg gcggcgcgag cgcgtggcag
     9481 atcgcaacgg caagcgcgtc ggcggcgtgg gtcgttgccg gctcgtcctg gagcccgagg
     9541 accaccttca ccattctctg catctgctgc ttgtcggcgc ggccgtagcc acagacgacc
     9601 tgcttcacgc gggccggcga atactcagcg cacccgaccc cggcgttggc ggccgcgacc
     9661 atgatggccc cgcgcgcctg tccgacggag agtgcggtgc gggcgtcgac gccgacgaac
     9721 gactcctcga ttgcgacggc atcgggctga tgaagcgcca cgagctcgac gacggcgtcg
     9781 aagatcgtct tcagacgcaa ctcggggcgt tcggccgcgg acgttttcca ccacccgtgg
     9841 tcgatggccc tgaggcgccc gtctttttcg tggacgatgc cgtacccgca agaagccgtc
     9901 cctggatcga tgcctatgac cttcacggtg agaggttagg cgtctaaccg gacggctcct
     9961 gctcgtatcc ttgcggccaa tgcccgggga tacctggctc accttccctg acgggacgga
    10021 gcacgagctg aaggagagcg tcacgatcgg acgcgacgcg tccagcgacc tcgcgttcga
    10081 gtcgccgacg gtgagccgcg agcacgcttc gctcacgttc cgcgacggtc gctggtacgt
    10141 cgaggatcgc ggcagcttca acggcacctt cctaaacgga acccgggtcc agcccgggac
    10201 tccactccct ctgcgccacg ccgatcgaat cgggatcggc tcggagaccg tcctcttctc
    10261 ctggccggcc cagctcgaag accccgatac gaccgagccg ctcgaggaga tcgcgcccac
    10321 ggcttcggct caactgtcgg ccttccagcg gcaggtcgtg cagggtcttt gcgggccgtg
    10381 gctggccggg gcgagcctcg agagcctgcc gtccaacgag cagatcgccg cccaactcgg
    10441 aacgcctggc gcgacgggca cggtgaaagc ggcactgcgc cgtatctacg cgaaggccgg
    10501 cctctccgac caaccggctc atgcgaagcg gcgggctctc tgtcgcgtgg cccggcaacg
    10561 cggctgggtc tgaagacaga gcgagggagc cgaagctccc tcgcgacccc tccgtaccag
    10621 ccctaaagcg taggcccacg ttcgcaaacc gtgggtggcc tcgtgggtca cccgagtgac
    10681 ccagttgctt ccggcggcta agcgaccgtc tcgaga
//
LOCUS       DASVEU010000100         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_69783,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000100 DASVEU010000000
VERSION     DASVEU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69783"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..631)
                     /locus_tag="VF225_03855"
     CDS             complement(173..631)
                     /locus_tag="VF225_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144420.1"
                     /translation="MIRQAGPQDVRFLRDMLKHAYHWRLNADPDLPVARYVNNWGRPG
                     DAGLVAWEAGPVGAAWYRLFPASAPGFGFVDEETPELTIAVVPSRRGGGLGGELMTAL
                     LDRARQQGHQAISLSAERGMTKLYERYGFVAVEEKDGTVTMKADLQTGPG"
     gene            complement(950..1288)
                     /locus_tag="VF225_03860"
     CDS             complement(950..1288)
                     /locus_tag="VF225_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144421.1"
                     /translation="MTAVWYQVRDLEAGRAFYTGKLGFTETYVDSDGTWVKLERDGME
                     IGLAEGEPQEDAGVAHVDVADVKAVAEELRNEGVEVGVVFELHGEMRLLDVFDPEGNR
                     LQFAQELSPG"
     gene            complement(1288..2562)
                     /locus_tag="VF225_03865"
     CDS             complement(1288..2562)
                     /locus_tag="VF225_03865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7144422.1"
                     /translation="MATEVKLPRLGQGMESGTIVKWLKSEGDKVEKGEPLYELDTDKV
                     TQEVEAEASGVLLKIAISAGEVEVGRTIAVIGEQGEQVELAPSEPAGNGSAALEVSGD
                     PEEEGSPARAREQDGEGGRRASAGMSDQVAEIRQPAGDNGGRVKASPLARRIARERGI
                     DLSAVAGTGPDGRVVAEDVERTAASGAPVALPSPALPSEGIEVQQLSSMRKTIARRLT
                     EAWQAPVFQLGITVDMSRALPLRERLVELHEEGVKPTISDLITKICAAALMRHRAVNA
                     LYKGDAVELYPTANIGIAVAVPNGLVVPVIPGCERKSIAEIAETRAALVGRAREGKLQ
                     QADLDGGTFTISNLGMYGIERFIAVLNPPQAAILAVGSIEDKVVPIDGQPAVRPRLEL
                     TLTCDHRAVDGATAAEFLRDVKAFLEEPGLAL"
     gene            complement(2552..>3084)
                     /locus_tag="VF225_03870"
     CDS             complement(2552..>3084)
                     /locus_tag="VF225_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7144423.1"
                     /translation="VRGLLWSSIYDDNPVVFFEHRTLYGIKEDVPDELEPIPLGQART
                     HRQGEDVTVIATGRLVHEALAAAKEAEQEGISVEVVDPRTLLPLDGDALISSVKKTNR
                     CVVAHEAVVRGGFGAEVAAVIQYGAFDWLDAPIERVGAKFAPLPFAPVMEEFVVPHAG
                     DVLHAIRRTVGRVDGN"
BASE COUNT          472 a         1110 c          974 g          528 t
ORIGIN      
        1 cgtcgggatg cacccgatcc gcaggcaggt gccaccgagc tcgggctcgc gctcgacgca
       61 ggcgactgtc gccccgagct gcgcagcccg gatcgcggcg gtgtaaccac ccggcccccc
      121 tccgagaact gcgacgtcga cttccatcag gctcgatcgt atcgaccggg cactaaccag
      181 gacccgtctg gaggtctgcc ttcatcgtga ccgttccgtc cttctcctcc acggcgacga
      241 agccgtagcg ctcgtagagc ttcgtcatgc cccgctcggc gctgaggctg atcgcctggt
      301 ggccctgctg gcgggcgcga tcgagcagag cggtcatcag ctcaccgccc aggccgcccc
      361 cgcggcggct cgggacgacg gcgatcgtga gctcgggagt ctcctcgtcg acgaagccga
      421 agcccggcgc gcttgcaggg aagaggcggt accaggccgc gccgaccggg ccggcctccc
      481 acgcaacgag cccggcatcg cccggccggc cccagttgtt gacgtatctc gccaccggga
      541 ggtcgggatc cgcattcagc cgccagtggt aggcgtgctt cagcatgtcg cggaggaaac
      601 ggacgtcttg cggcccggcc tggcggatca cggtcttgag tttaagccgc tctcactcga
      661 acgagtgatt ctccgaaaac caggcaagaa ggtcctacct gcggcatctt caaggttatg
      721 aaacgtgacg atgatcggcg gaccgagttc gggaccggcc tgcgggcccg tctgctcagg
      781 cgccgcgcgt tgcgactggg tcccggccgc gtacgcctgc ctgcgccgct tgttcagcgg
      841 ccttctgcca tctagctagc cacatagagc ctcggcgccc cgcagagcgc ccggcaaacg
      901 aaaatcagag gcgcatttcg tcacgttgca ccacgtcctc atgcagggct cagcctgggg
      961 agagctcttg ggcaaactga agacggttcc cctctgggtc gaatacgtcc agcaggcgca
     1021 tctcgccgtg caactcgaag acgacgccca cttctacacc ctcgttccgt agttcttccg
     1081 ctaccgcttt gacgtccgcc acatccacgt gggccacgcc cgcgtcctct tgtggctcgc
     1141 cctcggcgag accgatctcc atcccgtcgc gctccagctt cacccaggtc ccgtccgagt
     1201 cgacgtacgt ctccgtgaaa cccagcttgc cggtgtagaa cgcgcgtcct gcttcgagat
     1261 ccctgacctg gtaccagacc gcggtcacta caacgcgaga ccgggctctt ccaggaacgc
     1321 cttcacgtcg cgcaggaact ccgccgcggt ggctccgtcg accgcgcggt ggtcacacgt
     1381 gagagtcagc tccagccggg gccgcacggc gggttgaccg tcgatgggaa cgaccttgtc
     1441 ctcgatcgag ccgacggcga gaatcgcagc ctggggtggg ttgagcacgg cgatgaagcg
     1501 ctcgatgccg tacatgccga ggttcgagat cgtgaacgtg ccgccgtcga ggtccgcctg
     1561 ctgcagcttc ccctcgcgcg cgcgcccgac aagcgccgcg cgagtctcgg cgatctcggc
     1621 gatggacttt cgctcgcagc ccggaatcac cggcaccacg agaccgttcg ggaccgccac
     1681 ggcgatcccg atgttcgcgg tcgggtagag ctcgaccgcg tcacccttgt agagggcgtt
     1741 caccgctcgg tggcgcatca gggccgcggc gcagatcttg gtgatgagat cggagatggt
     1801 cggcttcacg ccctcctcgt gcagctccac gagacgttct cggagtggga gcgcgcggct
     1861 catgtcgacg gtgatcccca gttggaagac gggcgcctgc cacgcctccg tcagccggcg
     1921 cgcgatcgtc ttgcgcatcg aagacagttg ctgcacctcg atgccttcgc tcggcaacgc
     1981 aggagatgga agcgcgaccg gcgctcctga ggccgccgtg cgctcgacgt cctctgcgac
     2041 gacgcgaccg tcggggcccg tgcctgcgac cgcgctcagg tcgatgccgc gctcgcgcgc
     2101 gatgcggcgc gccagcgggg acgccttgac gcggccgccg ttgtcgccgg ccggttgcct
     2161 gatctcggcc acctgatcgc tcatcccggc agaggcacgg cggccgccct ctccgtcttg
     2221 ttcacgcgcg cgcgcgggag atccttcctc ctccggatcg ccagaaacct ccagggcggc
     2281 cgagccgttc cccgcaggct cgctcggcgc cagctcgacc tgctcgccct gctcgccgat
     2341 caccgcgatc gtcctgccga cctcgacctc gcccgccgag atcgcgatct tcagcaggac
     2401 gccggacgcc tcagcctcga cttcctgcgt caccttgtcc gtgtcgagct cgtagagcgg
     2461 ctcgcccttc tcgaccttgt cgccctccga cttgagccac ttcacgatcg tgccggactc
     2521 catgccctga ccgaggcgtg gcagcttgac ctcagttgcc atcgacacga cctacggttc
     2581 ggcgaattgc gtgaagcacg tcgcctgcgt gtgggacgac gaactcctcc atcacgggcg
     2641 cgaacggcag cggcgcgaac ttggcgccga cacgctcgat cggtgcgtcg agccagtcga
     2701 aggcgccgta ctggatcacg gcagcgacct ctgcgccgaa accgccccgc acgaccgcct
     2761 cgtgtgcgac gacgcagcgg ttcgtctttt tcacggagga gatcagcgcg tccccgtcga
     2821 gcgggagcag tgtccgcgga tcgacgacct cgaccgagat gccttcctgc tcggcctcct
     2881 tcgccgccgc gagcgcctcg tgcacgaggc gcccggttgc aatcaccgtc acgtcttcgc
     2941 cctgacgatg cgttcgcgcc tgaccgagcg gaatcggctc gagctcgtct gggacgtcct
     3001 ccttgatccc gtacagcgtc ctgtgctcga agaagacgac tgggttgtcg tcgtagatgg
     3061 aggaccagag caatccgcgc acgt
//
LOCUS       DASVEU010000101         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_653558,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000101 DASVEU010000000
VERSION     DASVEU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653558"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF225_03875"
     CDS             <1..620
                     /locus_tag="VF225_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX7144424.1"
                     /translation="LRGVLHQWAFVVFVGLAVLLIALASGAKERAGAATFAGALVAMF
                     GTSAVYHRVNWRSFAARMWLRRVDHAMIYVLIAGTYTPFALLALEGAWRWTILSIVWA
                     GALVAIVVMLAWVDAPKWLSAALGIALGWVGIVMVPELLDAIKPHGLVLLAAGGVLYT
                     FGAIVYARRRPDPMPTVFGYHEVFHACVIAAAACQYTVVAMLVAS"
     gene            complement(617..1159)
                     /locus_tag="VF225_03880"
     CDS             complement(617..1159)
                     /locus_tag="VF225_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144425.1"
                     /translation="MRRTRLAALSLILIAATAACGGGGGDGTETFEEEGYAFTFEYPG
                     SFEETSDVSYSSTAGGEAQDPIGLGLDDRNAIVVSRYELNAEVTSGNVSQVKPEFDDL
                     IRQLTGTEVSGKQVEIGGFPGFEYTFDLEDPPEGRSRFFVLFDGRTEYTLNCQSTPEK
                     RDEIEAACRQAIDTFAKTGD"
     gene            complement(1206..1547)
                     /locus_tag="VF225_03885"
     CDS             complement(1206..1547)
                     /locus_tag="VF225_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144426.1"
                     /translation="MSAVDREQRRKRQIARNEALFREVNERVEEVSSGRSTEMTEFLC
                     ECGNADCTEAIGLRDDEYQHVRSDPLLFAVMPGHEIPDVEEILDTNERFSVVRKHADE
                     GVIARLTDPRS"
     gene            complement(1544..1837)
                     /locus_tag="VF225_03890"
     CDS             complement(1544..1837)
                     /locus_tag="VF225_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144427.1"
                     /translation="MASRDDRMRENEELFRTANERLRRQVEDAVPDGQPVPFLCECMD
                     DLCMARIELTMGDYRHVRASDDTFAVAPGHAAPDGEVVVEERGEFHVVRKEAA"
     gene            1908..2387
                     /locus_tag="VF225_03895"
     CDS             1908..2387
                     /locus_tag="VF225_03895"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7144428.1"
                     /translation="MIRVVRDEGGGMAPMVHSVEIDRRREDVFAYVTDPTRFPEWQDA
                     VVRASPQGSGPLQQGSRISLTRRMGKREQTMTSELTEYSPPESYAFRVVDGPVRAIGK
                     GRFEPLGEGDRTRFTFELDFEGHGLGKLLVPLFVRRQAAKELPESHANLKKQLESGA"
     gene            2599..>3451
                     /locus_tag="VF225_03900"
     CDS             2599..>3451
                     /locus_tag="VF225_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7144429.1"
                     /translation="MTVCGRCGTEVPRGARFCPACGAPVVAGKRTEERKLATLLFADL
                     VGSTAIAASQDPERTRALLERFYSAMAEEIEQAGGTIEKFAGDAVVAVFGAPSALEDH
                     AERALHAALAMQHQLAQLFEGQLELRIGVNTGDVVVGQARDGSSFVTGDPVNVAARLE
                     QAADPGAILVGERTVAAARGAFEFEQPEAVVAKGKPEGVVASRLVRALSLMRPRGVSG
                     LARAFVGRDQELERLRDAYRAAVEQRRPRLVTILGDAGVGKTRLVRELWERLPEQKPE
                     PLRRTGRC"
BASE COUNT          491 a         1168 c         1209 g          583 t
ORIGIN      
        1 gcctgcgcgg cgtcttgcac cagtgggcgt tcgtcgtgtt cgtcgggctc gccgtcctcc
       61 tgatcgcact cgcgtcgggg gcgaaggagc gtgcgggcgc ggcgacgttc gcaggcgcgc
      121 tcgtggcgat gttcgggacg agcgcggtct accaccgggt caactggcgg agcttcgccg
      181 cgcgcatgtg gctgcgccgt gtcgaccacg cgatgatcta cgtcctgatc gcggggacgt
      241 acacgccgtt cgcgctcctg gccctcgagg gagcatggcg gtggacgatc ctctccatcg
      301 tctgggcggg cgccctcgtc gcgatcgtgg tcatgctcgc ctgggtcgat gcgccgaagt
      361 ggctgagcgc ggcgctcggg atcgcgctcg gctgggtcgg gatcgtcatg gtgccggagc
      421 ttctcgatgc gatcaaaccg cacgggctgg tgctgctcgc agccggcggc gtgctctata
      481 cgttcggggc gatcgtctac gcgcggcgcc gccccgaccc catgccgacg gtgttcgggt
      541 accacgaggt cttccacgcg tgcgtgatcg ccgcggcggc ctgccagtac acggtcgtcg
      601 ccatgctggt cgcttcctag tcccccgtct tggcgaacgt atcgatcgcc tgccggcacg
      661 ccgcttcgat ctcgtcacgc ttctcgggag tggactggca gttgagcgtg tactccgtcc
      721 tgccgtcgaa gagcacgaag aaccggctgc ggccctccgg cgggtcttcc aggtcgaacg
      781 tgtactcgaa gccggggaac ccgccgatct ccacctgctt cccgctgacc tccgttccag
      841 tgagctgccg gatcaggtcg tcgaactcgg gcttcacctg tgacacgttc ccggaggtca
      901 cttcggcatt cagctcgtag cggctgacga cgatcgcatt gcggtcgtcc aggccgagtc
      961 cgatggggtc ctgtgcttcg ccgccggcgg tggaggaata cgagacgtcg gacgtctcct
     1021 cgaaggagcc cgggtactcg aatgtgaacg cgtacccctc ctcctcgaac gtctccgtgc
     1081 cgtcgccgcc cccgccgccg caggcggccg tcgctgcgat caggatgagg gagagggccg
     1141 ccagacgtgt acgtcgcacc gcaggccgac cgtagggcga tcggcggcgg cggtcaagag
     1201 gcggatcagc tgcgcgggtc ggtcaaccgc gcgatcacgc cttcgtccgc atgcttgcgg
     1261 acgacgctga accgctcgtt cgtgtcgagg atctcctcca cgtccgggat ctcgtgaccg
     1321 ggcatgacgg cgaagaggag gggatccgac ctcacgtgct ggtactcatc gtcccgtagg
     1381 ccgatggcct ccgtgcagtc ggcgttcccg cactcgcaga ggaactcggt catctccgtg
     1441 gaccggccgc tgctcacctc ctcgacgcgc tcgttgacct ctcggaagag cgcctcattc
     1501 cgtgcgatct ggcgtttgcg acgctgctcg cggtcgaccg cgctcatgcc gcttccttcc
     1561 ggacgacgtg gaattcgccc ctttcctcta cgacgacctc tccgtcgggc gccgcatgcc
     1621 ccggcgcgac ggcgaatgtg tcgtcgctcg cccgcacatg ccggtagtcc cccatggtca
     1681 actcaatgcg cgccatgcag agatcgtcca tgcactcgca gaggaacgga accggttgtc
     1741 cgtccggaac ggcatcctcg acctgcctgc gaaggcgttc gttggcggtg cggaacaact
     1801 cctcgttctc gcgcatgcgg tcgtcccgag aggccatcgc gctggcgttc cccggccgcc
     1861 cccgcgcgaa accggagcgc gcctttactc cgtgtggggc gtgcgggatg atccgcgtcg
     1921 ttcgcgacga aggaggcggc atggccccga tggtgcacag cgtcgagatc gatcgaaggc
     1981 gcgaggacgt cttcgcgtac gtgaccgatc cgacgcgctt ccctgagtgg caggacgcgg
     2041 tcgtgcgcgc gagcccccag ggcagcggtc cgctccaaca gggctccagg atctcgctga
     2101 cccgccgcat gggaaagcgg gaacagacga tgacgagcga gctgaccgag tacagcccgc
     2161 cggagagcta cgcgttccgc gtcgtcgacg gcccggtccg cgcgatcggg aagggacggt
     2221 tcgagccgct cggtgagggc gaccggacgc gcttcacgtt cgagctcgac ttcgagggcc
     2281 acgggctcgg caagctgctc gtgccgctct tcgtccgcag gcaggcggcg aaggagcttc
     2341 ccgaaagcca cgcgaacctg aagaagcagc tcgagagcgg cgcgtagaca gccttgttgg
     2401 cggggccaag gggccagctc ggcgggggcc tggctacttg actcagggcc acgctgagcg
     2461 cacccggcga gggcgcgcta ggcgctttga gtcgtctgcg ctcgctcctc gtcgactgcg
     2521 gagcgcgtcg acgagatccc gctggccctt cgaaagtgtg ggctcactct cgtgccgctg
     2581 gtaagcgaca atcggggcat gacggtttgt ggccgctgcg gaaccgaggt ccctcgcggg
     2641 gcgcgcttct gtccggcgtg cggcgcccct gtggtggccg gcaagcgcac cgaggagcgc
     2701 aagcttgcga ctttgctgtt cgccgatctc gtcggctcga cggcaatcgc cgcttcccag
     2761 gatccggagc ggacgcgagc cctgctcgag cgtttctaca gcgccatggc cgaggaaatc
     2821 gagcaagccg gcggaacgat cgagaagttc gccggcgacg cggtcgtggc ggtcttcggc
     2881 gcaccgagcg cgctcgaaga tcacgccgag cgcgcgctgc acgccgcgct cgcgatgcag
     2941 caccagctgg cgcagttgtt cgagggccag ctcgagctcc ggataggcgt caacacgggc
     3001 gacgtcgtcg tcgggcaggc ccgcgacgga agctccttcg tcacgggcga cccggtcaac
     3061 gtagccgctc gactggagca agctgccgac cccggcgcga ttcttgtcgg cgagcgtacg
     3121 gtcgccgcag cgcgtggcgc cttcgagttc gagcagccgg aggcggtcgt cgcgaaaggt
     3181 aagccggagg gggtcgtagc aagtcgcctc gtgcgcgcgc tctcgctcat gcggccgcgc
     3241 ggtgtgagcg gcctggcacg cgcgtttgtc ggccgtgatc aggaacttga gcgccttcgg
     3301 gacgcatacc gcgcggccgt cgagcagcga cggccgcggc tcgtgacgat cctcggcgat
     3361 gcaggcgtcg gcaagacgcg gctcgtgcgc gagctctggg agcggctgcc cgagcagaag
     3421 cccgagccgc tccgacgcac cggccgctgc c
//
LOCUS       DASVEU010000102         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_70713,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000102 DASVEU010000000
VERSION     DASVEU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70713"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VF225_03905"
     CDS             complement(<1..1385)
                     /locus_tag="VF225_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144430.1"
                     /translation="MRSGSHRRDVRASALWGSGKRGTGSRSNALWGNGKRRTALLATL
                     VLTLVVPLGASAVPGPKDVQAFVAPGLLSSAQTSPTGTFSVIVQGKGGNQAAHAVADV
                     LGLSLKTARTFSSIDGVAVDLTGAQILAVAADKHVTAITADARVRLSAVAAPTNEKWP
                     FVTNVQKYWANGTTPAAPAATIAIVDSGIDATRPEFAGRIAASVNLSSLPGNSPGDGR
                     GHGTFVAGIAAADMQGKSGAAPGAKIVSLDVMDDHGMARTSDVIAAADWILANKAKYG
                     IRVANFSLHSSVANSFMYDPLDKAVEKLWFNNVVVVVAAGNYGNANGPSGVPFAPGND
                     PFVITVGASDTGKSVSTNDDVAAPWSAYGYTLDGFAKPDLAAPGRFMVGPVPVGSTLY
                     SDRPDHVVEPGYMELSGTSFAAPIVSGVSALILGKHPEYTPDQVKGALMVGTKPMPTA
                     TNLSEGVGEVNA"
     gene            1520..2896
                     /locus_tag="VF225_03910"
     CDS             1520..2896
                     /locus_tag="VF225_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX7144431.1"
                     /translation="MRIPIPLSAEIAVARAAGRLSRLARAGGGTTVPGKVLWKLDPGA
                     IDRLAARLPLGSAVISATNGKTTSAAMAASILRERLRLAHNSSGANLVSGVASTLLDA
                     RAAELGLFEVDEAALPEVLLRVKPKAVCLGNLFRDQLDRYGELEHVAQRWRSAVRALP
                     GDAGLAVNGDDPQVGELARERPGALVFGVDDLSAARPSLQHAADSKYCVRCGALYEYD
                     AAYVGHLGDYRCPACGHSRPPLNVVAREIEVNGLESVSFRLVTPSGTSKVRLRVPGLY
                     NVYNALAAASLALSLGATLEEIVSGLESFNAAFGRFERINVGDRGLLMLLIKNPAGAN
                     EAVRTIVDGGAPSVAVIALNDAIADGRDVSWIWDVDFEPLIGDLTRLVATGDRAAELA
                     LRFKYGGMDASAIDVVPSLESALDRGLELTPPGGELVVLPTYTAMLALRQIVSQRGFV
                     RPYWERAA"
     gene            2893..3645
                     /locus_tag="VF225_03915"
     CDS             2893..3645
                     /locus_tag="VF225_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144432.1"
                     /translation="MKIVVGHLYPDYLNIYADRGNIAVLSRRAAWRGHELEVREISVG
                     DPLAPGDHDLLYIGGGQDREQALVAHDLVAKSDGVRESVSGGAALLAVCGGYQLLGRS
                     YRDLHGAALPGIGVFPFDTVAGDTRMIGDVLLECELEPGQRWTLAGFENHAGRTRLDP
                     GAQPLGHVVAGFGNDGESGWEGCRVGRAVGTYLHGPLLPRNPWLADWLLAQALAHRYG
                     EVPELEPLADELEAEAHEVSARRAEARGGRRR"
     gene            complement(3751..4212)
                     /locus_tag="VF225_03920"
     CDS             complement(3751..4212)
                     /locus_tag="VF225_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7144433.1"
                     /translation="MRTAEPRIRVSAFLSWNGRVLLCRHEKPGKEYWLLPGGGVNGGE
                     SLVDALHRELAEEIGIDDQLPVEGPVAIVDSIAPQRSFAAKHVVHIIFAGDLGGRSLE
                     AVTSQDAAVRGHSLFELGDLDSVVLHPPIQRFLSRWQPGDPAVYLGALWAP"
     gene            complement(4209..>4569)
                     /gene="dut"
                     /locus_tag="VF225_03925"
     CDS             complement(4209..>4569)
                     /gene="dut"
                     /locus_tag="VF225_03925"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7144434.1"
                     /translation="LDLSACERVELAPGERALVGTGLAVAIPEGYAGFVQPRSGLAAR
                     HGLTVVNSPGLVDSGYRGELRVVLLNTDAQQPFLVEPGMRIAQLVVLPIPELELVEVE
                     ELPASERGVRGFGSSAT"
BASE COUNT          696 a         1543 c         1593 g          737 t
ORIGIN      
        1 gcattcactt cgccgacgcc ttccgagagg ttcgtggctg tcggcatcgg cttcgtgccg
       61 accatgagcg cgcccttgac ctggtcgggg gtgtattcgg gatgcttgcc gaggatcagc
      121 gcggagaccc cgctcacgat cggtgccgca aaggatgtgc ccgaaagctc catgtagccg
      181 ggctcgacga cgtgatcagg gcggtcgctg tagagcgtcg agcccaccgg aacgggaccg
      241 accatgaagc ggccaggagc ggccagatcg ggctttgcga agccgtcgag cgtgtagccg
      301 taagccgacc acggcgccgc gacgtcgtcg ttcgtcgata ccgacttgcc ggtgtcgctc
      361 gcgccgacgg tgatcacgaa ggggtcgttc cccggcgcga acggaacgcc gctcggcccg
      421 ttggcgttgc cgtagttgcc ggcggcgacc acgacgacga cattgttgaa ccagagcttc
      481 tcgaccgcct tgtcgagcgg gtcgtacatg aagctgttgg cgaccgacga atggagcgaa
      541 aagttcgcga cgcggatccc gtacttcgcc ttgttggcga ggatccagtc ggcggcggcg
      601 atgacgtcgc tcgtccttgc catgccgtgg tcgtccatca cgtcgagcga gacgatcttc
      661 gcgcccggcg cggcgccgct ctttccctgc atgtcggccg cagcgatgcc ggccacgaac
      721 gtgccgtggc cgcgaccgtc gccgggcgag ttgccgggga gcgagctgag attgacgctc
      781 gcggcgatac ggccggcgaa ctcgggccgg gtcgcgtcga tgccggagtc gacgatcgcg
      841 attgtcgccg caggtgctgc cggtgtggtg ccgttggccc agtacttctg gacgttcgtc
      901 acgaacggcc acttctcgtt cgtgggtgcg gcaacggcag agaggcgcac tcgcgcatcg
      961 gcagtgatcg cggtcacgtg cttgtcggcg gcgacagcga ggatctgtgc tcccgtgagg
     1021 tcgacggcca cgccgtcgat cgagctaaag gttcgggccg tcttgaggga gaggccgagg
     1081 acgtcggcga ctgcatgggc ggcctggttg ccgcccttgc cctgaacgat caccgagaag
     1141 gtgcccgtcg ggctggtctg ggcagaggac aggagcccgg gcgcaacgaa cgcctggacg
     1201 tccttcgggc ctgggacggc tgacgcgccc aggggcacga caagagtcag aaccagcgtc
     1261 gcgagaagtg ccgtgcgccg ctttccatta ccccagagcg cgttcgagcg cgatccggtc
     1321 ccgcgcttgc cgctacccca gagtgcgctc gcacgcacat cccgccgatg acttccgctc
     1381 cgcatctttc tcctttgccg cagttgacga accgcaccgt aggtagcacc cgggtgacgt
     1441 acctcaccct ttcgcggtag ccacgccaga agttcacccg tccgggggat cagcgccgct
     1501 cgatagcctc ctccgtggtt tgcgcattcc gattccgctg tccgccgaga tcgccgtcgc
     1561 gcgggcggcg ggccggttgt cgcgtctggc aagggccggc ggcggcacga cggtccccgg
     1621 aaaagttctc tggaagctcg atccaggcgc catcgatcgc ctggccgcga ggctgccgct
     1681 cggctcggcg gtgatctcgg cgacgaacgg caagacgacg agcgccgcga tggcggcgag
     1741 catcctccga gaacgcctcc gcctcgcgca caacagttcc ggggcgaacc tcgtctcggg
     1801 cgtggcctcg acactcctcg acgcacgcgc ggccgagctt ggcctgttcg aggtcgacga
     1861 ggcggcactg cccgaagtcc tcttgcgtgt caaaccgaaa gccgtctgcc tcggcaatct
     1921 cttcagggac cagctcgatc gctacggcga gctggagcac gttgcgcagc gctggcgttc
     1981 ggcggtgcgc gcgctacccg gcgatgctgg gctggcggtc aacggagacg atccgcaggt
     2041 cggagagctc gcgcgagagc gaccgggtgc gctggtcttt ggcgtcgacg atctctcggc
     2101 cgcgcggccg tccctgcagc acgccgcgga ctcgaagtac tgcgttcgct gcggagctct
     2161 ctacgagtac gacgcggcgt acgttggtca tctcggtgac taccggtgtc cggcctgcgg
     2221 ccacagccgc cccccgctga acgttgtcgc acgcgagatc gaggtgaacg ggctcgaatc
     2281 ggtctcgttc aggctcgtga ccccgtcggg gacgtcgaag gtccggctgc gcgtgcccgg
     2341 gctctacaac gtgtacaacg cgctcgccgc tgcttcgctc gccctgagcc tgggcgcgac
     2401 actggaggag atcgtctcag gcctcgagtc gttcaacgct gcattcggcc gcttcgagcg
     2461 catcaacgtc ggcgaccgtg ggttgctcat gctgctgatc aagaaccctg ctggcgccaa
     2521 cgaggcggtg cgcacgatcg tcgacggcgg ggcgccgtcc gtcgcggtga tcgcgctgaa
     2581 cgacgcgatc gcggacggcc gcgacgtctc gtggatctgg gacgtggact tcgagccgct
     2641 gatcggcgac ctcacgcggc tcgtggcgac cggggaccgc gccgcggagc tcgccctgcg
     2701 tttcaagtac gggggcatgg atgcaagcgc gatcgacgtc gtgccgtcgc tcgaatccgc
     2761 gctcgatcgc ggcctcgagc tcacgcctcc cggcggcgag ctcgtggtcc tccccaccta
     2821 cacggcgatg ctcgccttgc ggcagatcgt cagccaacgg ggcttcgttc ggccgtattg
     2881 ggagcgcgcg gcgtgaagat cgtcgtcggg cacctctacc cggactacct gaacatctac
     2941 gcggaccgcg ggaacatcgc agtgctgtcc cgccgtgcag cctggcgggg ccacgagctc
     3001 gaggtgcgag agatttcggt cggcgacccg ctggcgccgg gcgatcacga tctcctctac
     3061 atcggcggcg gccaggatcg cgagcaggcc ctggtcgcac acgatctggt cgcaaagtcc
     3121 gacggcgtgc gcgaatcggt ctcggggggt gcggcgttgc tcgcggtttg cggcggctac
     3181 cagctgctcg gacgctccta tcgcgacctc cacggggccg ccctgccggg gatcggtgtg
     3241 ttcccgttcg acaccgtcgc cggcgacacg cgcatgatcg gcgacgtgtt actggagtgc
     3301 gagctcgagc ccggccagcg ctggacgttg gcaggcttcg agaaccacgc gggacgcact
     3361 cgcctggatc cgggcgctca gccgctcggc cacgtcgtgg ccgggttcgg caatgacggc
     3421 gagagcggtt gggagggttg ccgcgtcggg cgtgccgtcg ggacgtacct ccacggcccg
     3481 ctcctgcccc ggaatccctg gctcgccgac tggctgctcg cgcaggcgct cgcgcatcgc
     3541 tacggcgagg tgccggagct ggagccgctg gccgacgagc tcgaggccga agcccacgaa
     3601 gtctcggctc ggcgggccga ggcgcgaggc ggccgtcgcc gctgaacgtc gcacccccac
     3661 ccagtgggtg gggagagatc ccgccaccct cacggcgagc ctagcgtccg aagtcctacg
     3721 agagtgcgtc gggactgcgc caaaagtgga tcaaggagcc cacagggcgc cgagatagac
     3781 agcggggtcg ccgggctgcc agcggctcag gaaacgctgg atcggcgggt gcagcacgac
     3841 gctgtcgaga tcaccgagct cgaacaggct gtgcccgcga accgccgcgt cctgcgatgt
     3901 cacggcttcg agcgatcgcc caccgagatc gccggcgaag atgatgtgca ccacgtgctt
     3961 ggccgcgaag ctcctctgcg gggcaatcga atcgacgatc gcgaccggac cttccaccgg
     4021 gagctgatcg tcgatgccga tctcctctgc gagctcgcgg tgcagcgcgt cgacgaggct
     4081 ctcgccgccg ttgacgccac cgccgggcag gagccagtac tccttgcctg gcttctcgtg
     4141 ccggcagagg agcacgcgcc cgttccagct caggaacgcc gagacacgaa tacgtggctc
     4201 cgcagtcctc acgtcgcaga cgatccgaac ccgcgcacac cgcgctcgct tgcgggaagc
     4261 tcttcgacct cgacgagctc cagctctggg atcggcagaa ccacgagctg cgcgattcgc
     4321 atcccgggtt cgacgaggaa cggttgttgc gcatcggtat tgagcaacac gactcgaagc
     4381 tcgccgcggt acccggaatc gacgagcccc ggggagttca ccaccgtgag gccgtggcgg
     4441 gctgcgagcc ccgagcgggg ttggacgaag cccgcgtaac cctccgggat cgcgacggca
     4501 agccccgtgc cgaccagggc tcgctcgccc ggcgcgagct cgacgcgctc gcacgcagag
     4561 aggtcgagc
//
LOCUS       DASVEU010000103         8920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_653782,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000103 DASVEU010000000
VERSION     DASVEU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653782"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1199)
                     /locus_tag="VF225_03930"
     CDS             complement(171..1199)
                     /locus_tag="VF225_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144435.1"
                     /translation="MASLRLDLSLPLRAFDLELALEAGPETVALVGPSGAGKTSVLRS
                     IAGLARPARGTISCGDEIWFDADRRIHRRPEERSVGFVFQEYALFPHLNVEQNVTFAG
                     ARADRLLWRLQIEHLAKAKPSELSGGERQRVALARALAREPKVLLLDEPMAALDPHTR
                     GKVRAELHDLLRELALPALLVTHDFEDAAALADRVGVLVAGRLRQLGAPAELLGAPAD
                     PFVAEFAGANVLDGIAESGADGLTRFHLDRERVVYSTDEARGRAALVLYPWDVSLARK
                     AADDSALNHLTGEIVSLVQVGNRVRVRLPFLTAEITAASAERLALRPGEAVVASFKAT
                     QARLLPAD"
     gene            complement(1142..1963)
                     /locus_tag="VF225_03935"
     CDS             complement(1142..1963)
                     /locus_tag="VF225_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144436.1"
                     /translation="MRALFAVLLAVATAVALAFLLLPVAAIFLRVPPGDLLGALGHNV
                     TRDALVVSIKTSAIAHAAVLLVGTPAAYLLARRRFPGRGAVLTLIELPLVLPPAVAGI
                     ALFAAFGRAGLLGDQLDALGVQIPFTQTAVVLAVIFVESPFYFRGAIAAFEAVDGTLV
                     DAARTLGASPARVFARVALPLAAGGLGAASALALARGLGEFGATLIFAGSLQGVTQTL
                     PLAIYAQFDQDFDTALAISALFIIVGALLLVTLRLYGWLRFASIFRSRFAPSTSS"
     gene            complement(1960..2685)
                     /gene="modA"
                     /locus_tag="VF225_03940"
     CDS             complement(1960..2685)
                     /gene="modA"
                     /locus_tag="VF225_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7144437.1"
                     /translation="MKRLLAVALAALALPASVSGGTPITVLAAASLTEVFPRIAASPH
                     YSFAGSDQLALQIRQGAPADVYAAASPKYTQLLYRDGFLLKPVVFATNKLIILVPKPN
                     PANINTAYDLRGGGIKLVIGDRTVPIGAYTRQILDTLGITADVMKNVVSEETDVKSIV
                     TKVALGEADAGLVYRTDARPVASRTKSIALPDWAQPPIRYEVAVVKTSSHLVAARAFV
                     KQVVSKRGRLLLGKAGFGLPKKK"
     gene            complement(2682..3053)
                     /locus_tag="VF225_03945"
     CDS             complement(2682..3053)
                     /locus_tag="VF225_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7144438.1"
                     /translation="MARTYTAGEAARALGISLDTLRRWDRQGRIRVQRDSANRRVVAA
                     REVERLRGEDRRNLSARNHLEGVVRGVKIDGLLAEIELDVAKGGRLVAVITRESAESL
                     GLAPGVSAAAVVKATSVMVER"
     regulatory      3131..3228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3220..4578
                     /locus_tag="VF225_03950"
     CDS             3220..4578
                     /locus_tag="VF225_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX7144439.1"
                     /translation="MQDTPQWGIEPVPERLRVLGPLDTTLLWTNLGVSLLVLVLPAYF
                     DLSLRDALAATLVGGLIGNLMLAVAALIGADARVPTMVLQRAPLGRRGSYLPTALNVL
                     QCLGWAIFELIVIATAAGLLCDKLFGFKGVWIWKLVFGAVAAMLALLGPVGFVRRFVR
                     KFAIWAVLASVVYLAWWIVDGAHLHRIWSEGGHKGSFWLAVDTVVAVTVSWAPLVADY
                     TRFSRDRRSAFFGVGIGYLLPTLFQFGFGSILVLSRGVDPNHPELILIAIAGGGLAAA
                     LALLALTVDETDEAFANVYSAAVSTQNLFPRISQRALIVGASVLATGGALAIDMRSYQ
                     RFLLLLGAVFVPLLGVLVADWLLAGAHYTPRHVFDGPAFRPANIVAWLAGFVVYEWLY
                     QPTDLGFWSRWVGHLPTPSYQIGASVPSFAVAFLLTAAAVTLGSSRAAARARREPLPG
                     RR"
     gene            4532..5308
                     /locus_tag="VF225_03955"
     CDS             4532..5308
                     /locus_tag="VF225_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7144440.1"
                     /translation="MPPLALVGNLSRDVVDGGPPRIGGAPYYAARAWRVLGTRGTIFT
                     RSGPEDRRAYVRHLTALGLPVVSLEGTATTSFSFHYQGDTRIMTVVQPGDTWIPEDAH
                     VVPPGGWIHVAPLLRSDFPAETLAALARGRHLSLDGQGLVRAAETGPLRLDADFDPAM
                     LEHVQILKLAEEEAEVVGAIDALGVPEVLVTFGERGSRVYYKGRSEEVGAWPVAVDPT
                     GSGDAFSAAYLAGRSQGLAPVSAARQATGVVGALLTGRLT"
     gene            5305..5688
                     /locus_tag="VF225_03960"
     CDS             5305..5688
                     /locus_tag="VF225_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144441.1"
                     /translation="MTAAVRTASGTYVVDVDAEEVIGAGDDFEPPRVTVELPRVVAAA
                     VAGATVVAVVDRRPPLAISNDAGRTWREAGGGLPAGFAVAIDEDNPDVMLFAGRNRLY
                     LSENGGIFWRALVPELPEIEAVAFL"
     gene            complement(5685..5882)
                     /locus_tag="VF225_03965"
     CDS             complement(5685..5882)
                     /locus_tag="VF225_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144442.1"
                     /translation="MKTIWTGRMAQAAGKYGENAPMAAVCCNACRTCVTTNILGVVTA
                     AIAGAGLGVTNFARRRFARPS"
     gene            complement(5920..6846)
                     /locus_tag="VF225_03970"
     CDS             complement(5920..6846)
                     /locus_tag="VF225_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7144443.1"
                     /translation="MATYRYVVVDVFTDKPLTGNQLAVFTDAREIPEEMLQPLAKEMA
                     FSETVFVYPPTRPEPVDEKALRGALDHEDDDETVFTVPPEAAGHARIRIFTPAAELPF
                     AGHPVLGSAFVLGMPLQLGEIRLETGAGIIPVTLEREGASIVFGRMQQPIPNWEPFAQ
                     AEELQQLLGVRSQLPVEIYDLGPHHVFLELGSEAEVAALEPDFGALARLTHSGVNCFA
                     GSGMHWKTRMFAPASGVAEDPATGSACGPLGVHLVRHGRIGFGEEIEVSQGAEIGRPS
                     TLYVRVDGSPAQIDRVEVGGSAVVVARGEFRL"
     gene            7003..7851
                     /locus_tag="VF225_03975"
     CDS             7003..7851
                     /locus_tag="VF225_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144444.1"
                     /translation="MSAISVRDLRKSYGTYEALRGISFEIKEGEVFGLLGPNGAGKTT
                     TIEILEGYRTRDGGDVEVLGFDPERAGSAFRERIGVVLQQSQLWPNLTVAETHRMFAG
                     YYEQPRNVDEVITLVGLDEKRDARVKTLSGGQKRRLDLGVALVGDPDLVFLDEPTTGF
                     DPAARRAAWDMIRSLRSLGKTILLTTHYLDEAEQLADRLAVLREGVIIREGTPAELTG
                     GSNETEIRYRKDGHDVVVRTTDPTKSLHKLTAEALAEGRELEGLSVHRPTLEDVYLSL
                     TAEHQE"
     gene            7848..8594
                     /locus_tag="VF225_03980"
     CDS             7848..8594
                     /locus_tag="VF225_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144445.1"
                     /translation="MRLFRHQLKWEQRLFWRSRESAVFVFLFPILLFALLTAVYNGHI
                     DGRPAAQVLLAGMIGYGAANTAFAGLAITLVSRREIGILKRIRATPLPPAVYLTAVLV
                     SIMIVFAIQVASLFALGRFLKSTPWPSHVLSLVLALALGAAAFAALGLAITGFIRSLE
                     GSSAVLNVIVLPTAFLSGSFGQTRHYPQVLRAIGDVLPLKYLLDLIKGIYLHGQQFWD
                     KPLAIAVLGAWGLFGMAVALRTFRWEPREG"
     gene            8625..>8920
                     /locus_tag="VF225_03985"
     CDS             8625..>8920
                     /locus_tag="VF225_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144446.1"
                     /translation="MQVENVIAFAVDQDPSGLEIRVNFGIFAGRDATSAELEELGKLL
                     VPEAGEVSIVGEQRHEISGDAEVVLHQVRVSVSPEIVPDDPAERKELCDRLVTLA"
BASE COUNT         1423 a         3054 c         2962 g         1481 t
ORIGIN      
        1 aagatgccgc gcacggcaag cgtcacggcg actaccgacg tccgcgttct cgtcatcacg
       61 gaacgggact tcggcacgct gctcaagcat tcgaacgaag tcggccgcgg cgtcgcggag
      121 gcgctggccg agcgcattgc ccccgattta ccggtctagg cgactcgcct ctaatccgcc
      181 gggagcaggc gggcctgcgt ggccttgaag ctcgccacga cagcctcccc ggggcgaagg
      241 gccagccgct ccgccgaggc ggcggtgatc tccgccgtga gaaacggaag ccgcacgcgc
      301 acccgattcc ccacctgaac gagcgagacg atctcgccgg tgaggtggtt cagggcggaa
      361 tcgtcggcag ccttgcgggc gagcgagacg tcccagggat agagaacgag cgccgcgcga
      421 ccgcgggcct cgtcggtcga gtacaccact cgctcccggt cgaggtggaa ccgcgtcagt
      481 ccgtccgcgc cgctttccgc aatgccgtcg agcacgttcg cgcccgcgaa ctcggccacg
      541 aacgggtccg ccggggcgcc caggagctcc gcaggtgcgc ccagctgccg caaccggccc
      601 gcgacaagca cgccgacgcg atcggcgagc gctgccgcat cctcgaagtc gtgcgtgacg
      661 aggagcgccg gcagcgccaa ctcccgcagc agatcgtgca gctcggcccg caccttcccg
      721 cgcgtatgtg gatcgagcgc cgccatcggc tcgtcgagta ggagcacctt cggctcgcgt
      781 gcgagcgcgc gagcgagcgc gacgcgctga cgctcaccgc cggacaactc ggacggcttc
      841 gccttggcga ggtgctcgat ctgcaaccgc cacaacagcc ggtccgcgcg cgcgccggcg
      901 aaggtgacgt tctgctcgac gttgaggtgc ggaaacagcg cgtactcctg gaacacgaaa
      961 ccgaccgaac gctcctcagg tcgacggtgg atgcgacggt cggcgtcgaa ccagatctcg
     1021 tccccgcagg agatcgtgcc tcgcgccggc ctggcgagcc cggcgatcga gcggagcacc
     1081 gacgtcttgc cggcaccgga tgggccgacg agcgcaaccg tctccggccc tgcctcgagc
     1141 gctagctcga ggtcgaaggc gcgaagcggg agcgaaagat cgaggcgaag cgaagccatc
     1201 cgtagagcct gagtgtgacg aggagcagcg ctcctacgat gatgaaaaga gcgctgattg
     1261 ccagcgccgt gtcgaagtcc tggtcgaatt gcgcatagat cgccagaggc agcgtctgcg
     1321 tgacgccttg caagctgccg gcgaagatga gcgtcgcgcc gaattcgccc agtccccgcg
     1381 ccagcgcaag cgccgaggca gcgcctaggc cgcccgcagc gagcgggagt gcgacgcgcg
     1441 cgaaaactcg ggccggagac gccccaagcg ttcgcgccgc atcgacgagg gtcccgtcga
     1501 ccgcttcgaa cgcggcgatc gcaccgcgaa aatagaacgg gctctccaca aagatgaccg
     1561 ccagcacgac ggccgtctgc gtgaacggaa tctgaacgcc aagcgcgtcg agctggtcgc
     1621 cgagtagacc ggcgcggccg aacgcagcga agagcgcgat gcccgccacc gcgggcggca
     1681 acacgagggg tagctcgatc agcgtgagca cggcgccacg gcctgggaag cggcgacgtg
     1741 ccaacaggta ggccgccggc gtgccgacta gtaggacggc agcgtgggcg atcgcactcg
     1801 tcttgatgct gacgacgagg gcgtcacgcg tgacgttgtg ccccagtgcg ccgaggagat
     1861 ctccgggcgg cacgcgcaga aagatcgccg cgaccggcag gagtaggaag gcgagtgcaa
     1921 ccgcagtcgc gaccgccaac agcaccgcga atagtgccct cacttcttct tgggcaggcc
     1981 gaacccggcc ttcccgagca gcagtcgccc tcgcttggaa acgacctgct tcacgaaggc
     2041 ccgggcggca accagatgtg agctcgtctt caccaccgcg acctcgtacc ggatcggggg
     2101 ctgcgcccag tcgggcaggg cgatcgattt cgtgcgcgag gccaccggcc tcgcgtccgt
     2161 tcggtagacc agccctgcat ccgcctcgcc gagggcgacc ttcgtcacga tgctctttac
     2221 gtcggtctcc tcgctgacga cgttcttcat cacatccgcg gtgatgccca gcgtgtcgag
     2281 gatctggcgc gtataggccc cgatcggaac ggtgcggtca ccgatcacga gtttgatccc
     2341 cccaccccgg aggtcgtagg ccgtattgat gttcgcgggg ttcggcttcg ggacgaggat
     2401 gatcaacttg ttggtggcga acacgaccgg cttcagcagg aagccgtcgc gatagagaag
     2461 ctgtgtgtac ttcgggctcg cggccgcata gacgtcggcc ggcgctcctt gccgaatctg
     2521 aagggcaagc tggtccgagc cggcgaagct gtagtgcggg ctcgcggcga tccgcgggaa
     2581 cacctccgtc agcgaggctg cggcgagcac ggtgatcggc gttcctccgg agacggatgc
     2641 gggcagcgcc agcgcggcga gtgcgaccgc aagcaggcgc ttcatcgctc gaccatcacg
     2701 gaggttgctt tcaccacggc agccgcggac actccgggcg cgaggccgag gctttctgcc
     2761 gactcccgtg tgatcacggc gacgagcctt ccgcctttcg ccacgtcgag ctcgatttcg
     2821 gcgagcaagc cgtcgatctt cactcctcga acgacgccct ccaggtggtt ccgggcgctg
     2881 aggttcctgc gatcctcgcc gcgcaaccgc tcgacctctc gggcggcgac gacgcgccga
     2941 ttcgcggagt cacgctggac gcggatccgc ccctgccggt cccagcggcg gagcgtgtcg
     3001 aggctgatcc cgagagcgcg cgcggcctcc cccgccgtgt aagtgcgtgc cacgaggcgg
     3061 cacgctactg catatgcatt acctaggcaa gctcctgtcg agcatcttct cgccgtcgcg
     3121 gccggatagg ctgacgacgc cggggtgcgc cgcgacggcg gcgctgagat cacacccgtg
     3181 gaacctgatc tggttcgtac cagcgaaggg agcaagcgca tgcaggacac accacagtgg
     3241 ggaatcgagc cggtgccgga gcggctgcgc gtcctcggcc cgctcgacac gacgctgctc
     3301 tggacgaacc taggcgtctc gctgctcgtg ctcgtcctgc cggcctactt cgatctgtcg
     3361 ctccgcgacg cgctcgccgc gacgctcgtc ggaggcctga tcggaaacct gatgctcgcg
     3421 gtcgccgcgc tgatcggtgc cgacgcgcgc gtgcccacga tggtgctcca gcgcgcgccg
     3481 ctcggacgcc gcggttcgta tctgccgacg gcactcaacg tgcttcagtg cctcggctgg
     3541 gcgatcttcg agctgatcgt gatcgcgacg gcagcaggcc tcctctgcga caagctcttc
     3601 ggcttcaagg gcgtctggat ctggaagctg gtcttcggcg cggtggccgc catgctcgcg
     3661 ctgctcggtc ccgtcggatt cgtgcggcgc ttcgtgcgca agttcgccat ctgggccgtc
     3721 ctcgcgtccg tggtttatct cgcctggtgg atcgtcgacg gcgcgcacct gcatcgcatc
     3781 tggtcggagg gcgggcacaa gggatcgttc tggctcgccg ttgacacggt cgtcgccgtc
     3841 acggtctcgt gggcgcccct ggtggccgac tacacgcgct tctcgcgcga tcgacgcagc
     3901 gcgttttttg gcgtcggcat cggctatctc ctgccgacgc tgttccagtt cggcttcggc
     3961 tccatcctcg tcctgtcgcg tggcgtcgat cccaaccacc ccgagctgat cctgatcgca
     4021 atcgctggcg gcgggctcgc cgccgctctt gcattgctcg ccttgacggt cgacgagacc
     4081 gacgaagcgt tcgccaacgt ctactccgct gcggtctcga cgcagaacct gttcccccgg
     4141 atctcgcagc gcgcgttgat cgtcggggct tccgtgctcg caaccggcgg cgcgctcgcg
     4201 atcgacatgc gcagctatca gcgcttcctg ttgctgctcg gagctgtctt cgtccccctg
     4261 ctcggcgtgt tggtcgccga ctggctcctc gccggcgccc attacacgcc gcgtcacgtc
     4321 ttcgatgggc ccgccttccg gccggccaac atcgttgcgt ggctcgcggg cttcgtcgtg
     4381 tacgagtggc tctatcaacc gaccgatctc ggtttctgga gccggtgggt cggtcacctg
     4441 ccgacgccgt cgtaccagat cggcgcctcc gtaccgagct tcgcggtcgc cttcctgctg
     4501 acggccgcgg ctgtgacgct aggctcgtcg cgtgccgccg ctcgcgctcg tcgggaacct
     4561 ctcccgggac gtcgttgacg gcgggccacc gcgaatcggc ggcgcaccgt actacgcggc
     4621 acgcgcttgg cgtgtgctcg gaacacgcgg gacgatcttc acacgcagcg gccctgaaga
     4681 ccgtcgcgcc tacgtccgcc atctgaccgc gctcggcctg ccggttgtct ccctcgaagg
     4741 gacggcgacc acgtcgttct ccttccacta ccagggcgac acgcggatca tgaccgtcgt
     4801 gcagccgggc gacacatgga tccccgaaga cgcacacgtc gtgccaccgg gaggctggat
     4861 ccatgttgcg ccgttactcc gatcggactt tcccgccgag acgctcgccg ccctcgcgcg
     4921 gggacgccac ctctcgctcg acggtcaggg actcgtccgc gccgccgaga cggggccgtt
     4981 gcgactcgac gccgacttcg atccggcgat gctcgaacac gtgcagatcc tcaagctggc
     5041 ggaggaggaa gcggaggtcg tgggtgcgat cgatgcgctc ggcgttcccg aggtgctggt
     5101 cacgttcggc gaacgtggct cgcgcgtgta ctacaagggt cgctccgaag aggtcggagc
     5161 gtggccggtc gcggtcgatc cgacgggatc cggcgacgct ttctccgctg cctacctcgc
     5221 aggcaggtcc caggggttgg caccggtgtc ggcggcaagg caggcaaccg gcgtcgtggg
     5281 tgcccttctg accggaagat tgacttgacc gcggctgtac gaacggcctc cgggacctac
     5341 gtcgtcgacg tggacgccga ggaggtcatc ggcgcgggcg acgacttcga gccgccgcgc
     5401 gtcaccgtcg agcttccgcg cgtcgtcgca gcagcggtcg caggtgcgac cgtcgtggca
     5461 gttgtcgacc ggcgcccgcc gctcgcgatc tccaacgacg cgggccgcac gtggcgtgaa
     5521 gcagggggcg gcctacccgc cggcttcgcc gtggcgatcg acgaggacaa ccccgacgtg
     5581 atgctctttg caggacgcaa ccgcctgtat ctgtccgaga acggcggcat cttctggcgc
     5641 gcactcgtgc cggagctccc ggagatcgaa gcagtcgcgt tcctctagga gggtcttgcg
     5701 aaacgccggc gagcaaaatt cgtcacgcca agcccggcgc cggcgattgc ggccgtcacg
     5761 acgcccagga tgttcgtcgt gacgcacgtt cggcaggcgt tgcagcagac ggcggccatc
     5821 ggcgcgtttt cgccgtattt cccggccgcc tgggccatgc ggcccgtcca gatcgtcttc
     5881 acaaggagat cgtagccggg ctaggcggcg aagtctcccc tagagacgga actcgccacg
     5941 cgccacgact acggccgagc cgccgacctc gactcggtcg atctgtgccg gcgatccgtc
     6001 cacccgaacg tagagcgtcg acggccgccc gatctccgcg ccttgcgaga cctcgatctc
     6061 ttccccgaag ccgatgcggc cgtggcgcac gaggtgcacg ccgagcggtc cgcacgcgga
     6121 gccggtcgca ggatcctcgg caacgccgct cgcgggcgcg aacatccgtg tcttccaatg
     6181 catgcccgac ccggcgaagc agttcacgcc gctgtgcgtc agccgcgcaa gcgcaccgaa
     6241 gtcgggctcg agcgcagcaa cctccgcttc gctaccgagc tcgaggaaga cgtgatgcgg
     6301 accgaggtcg tagatttcga cgggaagctg cgagcgcacg ccgagcagtt gctgcagctc
     6361 ctcggcctgc gcgaacggct cccagttcgg gatcggctgc tgcatccggc cgaacacgat
     6421 gctcgcccct tcacgctcga gcgtcaccgg aatgatcccc gcacctgtct ccaggcgaat
     6481 ctcgccgagc tgtagcggca tcccgaggac gaacgccgac ccgagcacgg gatgtcccgc
     6541 aaaggggagc tcggccgccg gcgtgaagat gcgaatccgc gcatgaccgg cggcttccgg
     6601 aggcacggtg aagaccgtct catcgtcgtc ctcatgatct aacgcccccc gtaatgcctt
     6661 ttcgtctacc ggctcgggtc gcgtaggcgg gtagacgaac acggtctccg aaaaggccat
     6721 ttcctttgcg aggggttgga gcatctcctc cggaatctcg cgggcgtccg tgaagaccgc
     6781 cagctggttc ccggtgaggg gcttgtccgt gaagacgtcc actacgacgt agcggtaggt
     6841 cgccatagcg gctcagtaaa ccagctcgcg gagacaacga actcaagttt ccgaaacacc
     6901 tcacggatga gcgacggtgg ccgcgtgtga agacgtcccc gactacctag cgcagggtcg
     6961 gcacaggacg atcctctacc atgatcaggt gagccggcgg ccgtgagcgc gatctccgtc
     7021 cgcgacctca ggaagtccta cgggacatac gaggctctcc gcgggatcag cttcgagatc
     7081 aaggagggcg aggtcttcgg cctcctcggc ccaaacggcg ccggaaagac gacgacgatc
     7141 gagatcctcg agggttaccg cacaagggac ggcggtgacg tcgaggttct cggcttcgat
     7201 cccgagcgtg ccggctcggc gtttcgagag cgcatcggcg tcgttctgca gcagtcgcag
     7261 ctctggccga acctgacggt cgccgagacg caccggatgt tcgccggtta ctacgaacag
     7321 ccgcgcaacg tcgacgaggt gatcacactc gtcggcctgg acgagaagcg cgacgcgcgc
     7381 gtgaagacgc tgtcgggagg gcagaagcga cgtctcgacc tcggcgttgc gcttgtcggc
     7441 gatcccgatc tcgtcttcct cgacgagccc acgacaggct tcgatcctgc cgcgcggcgt
     7501 gcagcctggg acatgatccg ctcgctgcgt tcgctcggca agacgatcct gctgacgacg
     7561 cactacctcg acgaggcaga gcagctcgcc gaccggctcg cggtcctgcg tgaaggcgtg
     7621 atcattcgcg aaggcacgcc cgctgaactg accggtggct cgaacgagac cgaaatccgc
     7681 taccgcaagg acgggcacga cgtcgtggtc cggacgaccg accccaccaa gagcttgcac
     7741 aagttgacgg ccgaggcact ggccgaagga cgggaactgg aaggcctctc cgtgcaccgg
     7801 cccaccctgg aagacgtcta cctctcgctg accgcagagc accaggaatg aggctcttcc
     7861 ggcatcagct gaagtgggag cagcgcctct tctggcgcag ccgcgagtcg gcggtgttcg
     7921 tgttcctctt ccccatcctg ctctttgcgc tcctgaccgc ggtctacaac ggccacatcg
     7981 acgggcgtcc cgcagcgcag gtcctgctcg ccgggatgat cggttacggc gcggcgaaca
     8041 cggcgttcgc cgggctcgcg atcacactgg tctcccgacg ggagatcggc atcctgaaac
     8101 ggatccgggc aacacccctt ccgccggcgg tgtacctcac cgcggtgctc gtctcgatca
     8161 tgatcgtctt tgcgattcag gtggcgtcac tcttcgcgct cggccggttt ctgaagtcga
     8221 ccccgtggcc gagccatgtg ctctcactgg tcctggcgct ggcactcgga gccgctgcgt
     8281 tcgcagcgct cggcctggcg atcaccggtt tcatccgctc gctcgagggc tcgtctgcgg
     8341 tgctcaacgt catcgtgctg ccgacggcct tcctcagtgg atcgttcggc cagacccgtc
     8401 actatccgca ggtcctgcgt gcgatcggcg acgttctccc gctgaagtac ctcctggacc
     8461 tgatcaaggg catctatctc cacggccagc agttctggga caagcctctg gccatcgccg
     8521 tcctcggtgc ctggggatta ttcgggatgg ctgtggcatt gcgcactttc cgctgggagc
     8581 cacgagaggg ttagccccgg gccgagtcgg ctatacggct tgtgatgcag gtcgaaaacg
     8641 tcattgcgtt cgccgtcgac caggacccct ccggccttga gatccgggtc aatttcggca
     8701 tctttgcggg ccgcgacgcg acttccgccg agctcgagga gctcggcaag ctgctcgtgc
     8761 cggaggccgg cgaggtctcg atcgtcgggg agcagcgcca cgagatctca ggggatgccg
     8821 aggtcgtcct gcaccaggtg cgtgtctccg tctcgccgga gatcgtgccg gacgatcccg
     8881 ccgagcggaa ggagctctgc gatcggctgg tcacactggc
//
LOCUS       DASVEU010000104         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1626093,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000104 DASVEU010000000
VERSION     DASVEU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626093"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VF225_03990"
     CDS             complement(<1..810)
                     /locus_tag="VF225_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7144447.1"
                     /translation="MREAGGRGWPGVELRHLLTLEAVAEEASFRRAARRLGYSQSAVS
                     QQVAALERAIGHRVVDRGVGERVALTAVGAVVMEHARAIDDHLRAAHADVTRLSLDGA
                     RLRVGTSPGVGGAVLAHVMAQFEHAAPGVALLVEEHPRVADPLEALRLGDLDLAFAVL
                     PLPPGPFIRTKLFDESFTLVVRADSALVRARRAPTADDLGTLPLITSKDCPATACALE
                     SVRAAGVEPTVVFRSSDIATVQQLVAAGFGAGLIPTTSTLSGDLVRVDLDGL"
     gene            complement(884..1483)
                     /locus_tag="VF225_03995"
     CDS             complement(884..1483)
                     /locus_tag="VF225_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7144448.1"
                     /translation="MGTTNPTSELTTTEAAVLGMLLHGELSGYEMQKHARHGIGYLWA
                     PARTRIYAALPQLVGRGLATVRSVSQTTKPDKQLYKLTNEGRAALRAWLDRPFADPQS
                     DKNPLLLKLFLGREADPDVLAAQVRERGDAARTLLRELEALDADVPLGDPDDFFQSLT
                     RRWGLAYARALASWADDTERDLERWQTARRSSRTRERRS"
     gene            1798..>2858
                     /locus_tag="VF225_04000"
     CDS             1798..>2858
                     /locus_tag="VF225_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7144449.1"
                     /translation="MQRRSLVVLAGVFTLLASLTLGPAASAGSERLSAGTVVFIHDQE
                     PPSLRADWVDNSLYATSLITNNVWYGGQIRDAGAEWQTRLFTGAPKLVKKSPLTASFQ
                     YSPKANWSDGTPVTCADWRASWQMWIDPKNNVASRTGYEDVRSVTCKGKSGTVVFKKV
                     YADWQSLLNAGAYQAKTLNAIADKNTSFDSSIPVSSGPWKFQSWQKGVQITVVKNPRF
                     TAAPAMKLDRIVFRYIADTNARFQALKAGEGQVMEPQAQLQIADFVKDKNFNVESKAG
                     YSFEHLDIQYGPQGAPALRAAYVRQALIYGINRPQIATALYSTIAPGLPTLQSLMFKT
                     FEKGYTKHFQKYVFNQSKV"
BASE COUNT          494 a          984 c          969 g          411 t
ORIGIN      
        1 caggccgtcg agatccactc gaacgagatc gccggaaagc gtgctcgtgg tggggatcag
       61 cccggccccg aaaccggccg caaccagctg ctgaaccgtc gcgatgtcgc tcgagcgaaa
      121 gacgacggtg ggctccacgc cggccgcccg gacgctctcg agggcacacg cggtcgcggg
      181 gcagtccttc gaggtgatca gcggcagcgt gcccaggtcg tccgccgtcg gcgcgcgcct
      241 ggctcgcacc agggccgagt ccgcgcgcac gacgagcgtg aacgactcgt cgaacagctt
      301 cgtccggatg aacgggcccg gcggcagggg cagcaccgcg aacgcgaggt cgaggtcacc
      361 gagccgcagg gcctccagcg ggtcggccac acgcgggtgc tcctcgacga gaagcgcaac
      421 gcccggcgca gcgtgctcga actgcgccat gacgtgcgcg agaacggcgc cacccacgcc
      481 cggggacgtt ccgacacgca gtctcgcgcc gtccagggag agtcgcgtga catccgcgtg
      541 cgcggcccgc aggtggtcgt cgatcgctcg cgcatgctcc atgaccaccg cgccgacagc
      601 cgtgagcgcg actcgctccc ccacgccgcg gtcgacgaca cggtgtccga tggcgcgttc
      661 gagcgccgcc acctgctggc tcacagccga ctgggagtaa ccgagacggc gcgcggcacg
      721 cctgaaggac gcctcctccg ccacggcctc gagcgtgagg aggtggcgca gttcgacccc
      781 gggccagccc cggccgccgg cctcacgcac tgtgccccgc ccgcagtccg cttagtccca
      841 ttgggactat gacgtcggct tgcgtctcga agtttgtgac gcgctaggag cgacgctcgc
      901 gggtgcgcga cgaacgccgc gcggtttgcc agcgctcgag gtcgcgttcc gtgtcgtccg
      961 cccacgacgc cagcgcccgc gcgtacgcga ggccccagcg ccgcgtcagc gactggaaga
     1021 agtcgtccgg gtcgccgaga gggacgtcgg cgtcgagcgc ttcgagctcg cgcaggagtg
     1081 tgcgcgcagc gtcgccccgc tctcgaacct gcgccgcgag cacgtccggg tcggcctcgc
     1141 gacccagaaa gagcttcaga agaagcggat tcttgtccga ctgggggtcg gcgaagggcc
     1201 ggtcgagcca tgcacgcagc gctgctcgcc cctcgttcgt gagcttgtag agctgcttgt
     1261 cgggcttggt cgtctgcgac acgctccgga cggtcgcgag cccgcgaccg acgagctgcg
     1321 gcagagccgc gtagatgcgc gtcctcgccg gcgcccagag gtagccgatg ccgtgccgcg
     1381 cgtgcttctg catctcgtag ccggacagct cgccgtgaag gagcatcccc agaacggccg
     1441 cctcggtcgt cgtgagctcc gacgtcgggt tagtagtccc catgggacta gcgtagccaa
     1501 cgagcgtctc gatcacccgc tcacaggcct cgagccggga gggcaagggc agacatgcgc
     1561 agtcgtctcg cgaaccggct cgaggccgcg tggcggacct cggatggagg atgggacgaa
     1621 cctagcccgc cgcgatcgcc gcaggtattc gcgatccgaa tcgcggctgc gcgaatcgtg
     1681 agcggcgatc agcgagacgg aactcgaggt cacgaatcgt catcgcgagc gcacgacacg
     1741 ggccgtctaa ccgagagcgg aggtcatgag gacgtccgcg ccgatcggag gagtcacatg
     1801 caaagacgct ctctcgtcgt gctcgcgggg gtttttacac tgctcgcgag cctgaccctc
     1861 gggcctgccg cgtcggccgg ctcggaacga ctcagcgccg gaaccgtggt cttcatccac
     1921 gaccaggagc cgccctcgct ccgtgcggac tgggtcgaca actcgctgta cgcgaccagt
     1981 ctcatcacga acaacgtctg gtacggcggc cagattcgcg atgcgggcgc cgagtggcag
     2041 acgcgcctgt tcacgggcgc gccgaagctc gtcaagaaga gcccgttgac ggcctcgttc
     2101 cagtactcgc ccaaggcgaa ctggagcgac ggcacccccg tcacgtgcgc ggactggcgt
     2161 gcgtcgtggc agatgtggat cgacccgaag aacaacgtgg cctcgcgcac gggctacgag
     2221 gacgtgcggt cggtgacctg caagggcaag tcgggcaccg ttgtcttcaa gaaggtgtac
     2281 gcggactggc agtcgctcct gaacgcgggc gcctaccagg cgaagacgct caacgcgatc
     2341 gcggacaaga acacctcgtt cgacagctcg attcccgtgt cgagcggccc gtggaagttc
     2401 cagagctggc agaagggcgt ccagatcacc gtggtgaaga acccgcggtt cacggccgct
     2461 ccggcgatga agctcgaccg catcgtcttc cgctacatcg ccgacacgaa cgcgcgcttc
     2521 caggcgctga aggccggcga ggggcaggtc atggagccgc aggcgcagct ccagatcgcc
     2581 gacttcgtca aggacaagaa cttcaacgtc gagtcgaagg cgggctacag cttcgagcat
     2641 ctcgacatcc agtacggccc acagggtgcg ccggcgcttc gcgcggcgta cgtccgccag
     2701 gcgctcatct acggcatcaa ccggccgcag atcgccacgg ccctctactc gaccatcgct
     2761 cccggcctgc cgacgctgca gagcctgatg ttcaagacgt tcgagaaggg ctataccaag
     2821 cacttccaga agtacgtgtt caaccagtcc aaggtcat
//
LOCUS       DASVEU010000105         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1041834,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000105 DASVEU010000000
VERSION     DASVEU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041834"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF225_04005"
     CDS             complement(<1..843)
                     /locus_tag="VF225_04005"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7144450.1"
                     /translation="MERSLPWAWYADPEVLRREGERIFARAWQYVGHTGQIAETGFFA
                     STAGQIPVVVTRARDGELRAFLNVCRHRGHIVASGSGTRETLQCPYHAWTYALDGKLR
                     AAPRSDREPGFDTDELGLRQIQVDTFGPFVFVNPDQDAATLADALGEVPAQLAEILDV
                     DSLEFRFRSEFELEANWKISCENFLECYHCAVAHPGFSAAVDVSPDAYRLEAKGLVSS
                     QFGPLRHNGNSFLADGEVPRSQFHFLWPNFGLNVFPGHPNLSCGPMLPIGPERTARFL
                     DYFFA"
     gene            869..>2528
                     /locus_tag="VF225_04010"
     CDS             869..>2528
                     /locus_tag="VF225_04010"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7144451.1"
                     /translation="MAVTKTRNLPLMEQFFAVRRFSSALDFTADGAHLLFASNISGQF
                     NLWRVPVEGGWPDQLTAFTDETVRGVGVSPTDGRIVLCADHDGDEFHQLYLLDSDRGW
                     PRKLTDEPEVQHYVGSTAWSPDGTKFAYAANARKPTDMEVWVRDAETGETRPVFGEGK
                     FSFPGRFSPDGSKLLAIDFRNNSDVSIYLIDLDSGETRELTPHDDDAIFFPGPWASDG
                     SGFYLVTDVGSEFRGLAFYDLASDSHDWVEEPTQDVDDVTMSADGRVLGWLVNDQGYD
                     RLRLRDLETGQDLPEPDLPAGARPHLTGAEPPLALSRDGSHAALIAASPRRPPEAWVV
                     ETDRGKARAVTDSRLGGLLENDLVDAELVSYPSFDGRDIPAWLYRPNVDGKVPVVLSI
                     HGGPEAQERPVYQPLYQYLLSRGIAVLATNIRGSTGYGKSYQRLVQRDWGGGDMQDWE
                     HAVKWLRDQDWVEPERIGVYGGSYGGFAVLTCVTRLPQYWAASVDIFGPSNLVTFAKA
                     VPPTWKRFIARFVGDPETEADFLMERSPITYVDNVRTPLLVIQGA"
BASE COUNT          414 a          840 c          862 g          412 t
ORIGIN      
        1 cgcgaagaag tagtcgagga aacgcgccgt ccgctcgggg ccgatcggga gcatggggcc
       61 gcaggacagg ttcgggtgtc ccggaaagac gttcaggccg aagttcggcc agaggaagtg
      121 gaactggctg cgcggcacct cgccgtcggc gagaaacgag ttgccgttgt ggcggagggg
      181 tccgaactgg ctggacacga gccccttcgc ctcgagccgg taggcgtccg gcgagacgtc
      241 gacggcggcg ctgaagcccg ggtgcgcgac ggcgcagtga tagcactcga ggaagttctc
      301 gcacgagatc ttccagttcg cttcgagctc gaactcgctg cggaagcgga attcgagcga
      361 atcgacgtcg aggatctcgg cgagctgcgc cgggacttcg ccgagcgcgt cggcaagcgt
      421 ggccgcgtct tgatcgggat tcacgaacac gaacgggccg aaagtgtcga cctggatctg
      481 cctgagcccg agctcgtcgg tgtcgaaccc aggctcgcga tccgagcgtg gcgcagcgcg
      541 tagctttccg tcgagcgcgt aggtccacgc gtggtaaggg cattgcaacg tctcgcgcgt
      601 cccggatcca ctcgccacga tgtgcccgcg gtggcggcag acgttgagaa aggctcggag
      661 ctccccgtcg cgcgcgcgtg taacaaccac agggatttgg ccggcggtgg acgcgaagaa
      721 gccggtctcc gcgatctggc ccgtgtggcc cacgtattgc caggcgcgcg cgaagattcg
      781 ctcgccctca cgccggagga cttcgggatc ggcgtaccag gcccagggca atgacctctc
      841 catagcgcag aactgtaatc tgcgcgccgt ggccgtcacc aagacgcgga acctcccgct
      901 catggagcag ttctttgccg tccgccgctt ctcctcggca ctcgatttca cggccgacgg
      961 cgcgcacctc ctcttcgcca gcaacatctc cggtcagttc aatctctggc gcgttcccgt
     1021 cgagggtggc tggcccgacc agctgaccgc attcacggac gagaccgtgc gcggtgtcgg
     1081 cgtctcaccg accgacggtc ggatcgtcct ctgcgccgat cacgacggcg acgagttcca
     1141 tcagctttac ctcctcgatt ccgaccgcgg ttggccgcgg aagctcacgg acgagccgga
     1201 ggtccagcac tacgtcggca gcaccgcgtg gtcgccggac gggacgaagt tcgcctacgc
     1261 cgccaacgcg cgcaagccga ccgacatgga ggtctgggtg cgcgacgccg agacgggcga
     1321 gactcgtccc gtcttcggtg aaggcaagtt ctcctttccg gggcgctttt ctcccgacgg
     1381 ctcgaagctc ctggcgatcg acttccggaa caacagcgac gtgtcgatct atctcatcga
     1441 tctcgactcc ggagaaaccc gcgagctcac gccgcacgac gacgacgcga tcttcttccc
     1501 ggggccatgg gcgtcggacg gttcgggctt ctacctcgtc acggatgtcg gcagcgagtt
     1561 ccgcgggctc gcgttctacg accttgcctc cgatagccac gactgggtcg aggaaccgac
     1621 acaggacgtc gacgacgtga ccatgtcggc cgacggccgc gtgctcggct ggctcgtcaa
     1681 cgaccagggc tacgaccggc tgcggctgcg agacctcgag accggacagg atctcccgga
     1741 gcccgatctt cccgccggtg cccgtcccca tctgaccggg gccgagccgc cgctcgccct
     1801 atcgcgcgac ggctcgcacg cagcgctgat cgcggccagc ccgaggcgcc cgcccgaggc
     1861 ctgggtcgta gagaccgatc gggggaaggc tcgggccgtg accgacagcc ggctgggcgg
     1921 cctgctggag aacgacctcg tcgatgccga gctcgtgagc tatcccagct tcgacggccg
     1981 cgacattccc gcctggctgt acaggcccaa cgtcgacgga aaggtgcctg tggtcctgtc
     2041 gatccacggt ggtcccgagg cgcaggagcg gccggtctac cagccgctgt accagtacct
     2101 gctctctcgc ggcatcgcgg tgctcgcgac gaacatccgc ggctcgacgg gttacggcaa
     2161 gtcgtaccag cggctcgtgc agcgcgactg gggcgggggc gacatgcagg actgggagca
     2221 cgccgtgaaa tggctccgcg accaggactg ggttgagccg gagcgcatcg gcgtgtatgg
     2281 cggctcgtac ggcggcttcg ccgtgctgac gtgcgtgacg cggctgccgc agtactgggc
     2341 ggcgtcggtg gacatcttcg gcccatcgaa cctcgtcacg ttcgccaagg cggtcccgcc
     2401 gacgtggaag cggttcatcg cgcggttcgt cggcgatccg gagacggaag cagatttcct
     2461 gatggagcgt tcgccgatca cgtacgtcga caacgtgcgg acgccgctgc tcgtgatcca
     2521 gggcgcaa
//
LOCUS       DASVEU010000106        20956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_71175,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000106 DASVEU010000000
VERSION     DASVEU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 20956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20956
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71175"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3534
                     /locus_tag="VF225_04015"
     CDS             <1..3534
                     /locus_tag="VF225_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7144452.1"
                     /translation="LLVLLPVFGLRAFSAIDDRRAAIRGAEGDAYDTARIVAQDQQGL
                     LEQSRQQLFTFVQLVALSSGRPIEPRTCKPALVTFMKRLPNYVDLGIVAPDGKVLCAA
                     TTKAGADFAREPFVRRALQSRQFAIGDYELRPAVGKPTISLAYPVVVRGRVSSIMFAT
                     LDLAALNRRAAETPLARGWVLNIIDNRGTILVRQPSSSQWIGRTVRDAPLMRAVLREG
                     KGRVKARGVDGVTRFYGFTGLFGLESGSDLHVTVGIPKDLALAASTRKAKRDFALLAL
                     VALATLLIAWLSSRMFFLRPLDALTRTAQRLRAGDLGARTGLRERAGELGELARTVDE
                     MAESLELREREINRLNEDLERRVADRTVELEAARDELESQNAELELQTVELEDHQTEL
                     ASANDELEAQRAELEVLLEDLAHEKERLELLYAFGEMLARETESGGLADNVMRELGDL
                     AEAEVGVLYVAAPEKDYELVLATTRGIDHAGLPERITPGKGLAGRAVDELRRIVVARG
                     DSELRVESFGKEVEVRHEVHLPLSSAGRTLGVITLGRLGDRPFTEEQLETLDHLAGQT
                     AVAISNKLAFREALRLGEITQAVIEATQDGIALANQEGETILMNARLKHMLSDLLELP
                     PGRTVQEYMEIMPERVTEPERYREVTAAIARDPEYEGVTEFELRSGRSFTRYTATVRG
                     ADGQQIGRIFVVRETTADREVERLKSELVATVSHELRTPLSSIMGFAELLADRDYDEA
                     TRKRFLATIHGEAARLTALVNEFLDLQRIEDGSFTLALEAFDLDALVRDQVELFSAQS
                     DAHTLDLELPDGAVPVVGERDRIKQVLGNLLSNAIKYSPHGGVVQVRLQPNGTAVRLS
                     VSDEGLGIPGTQQGGVFSKFFRADSSDTREIGGTGLGLALCKDIVEAHAGQIGFTSVE
                     GKGSTFWFELPSPSSSNGRRDDRPVVLVVEDDPEAASLLSHYLLEADYAVVTTVTGEE
                     ALAHIAQHRPDAICLDMTLAGELSGWDVLSRLKESPATSGIPVVICTAGNGRADASAL
                     GAADFLAKPFAAGELHAALQRVLPTGRGSVLIVDDEESVRALVLETLSGTGYELREAA
                     DGEEALERIAARRPDAIVLDLTMPKLDGFAVLERLQGDPETRRIPVVVLTARALLAAE
                     RSLLQRRAVNLLEKNDYSGEELRGLVQRALG"
     gene            complement(3502..3888)
                     /locus_tag="VF225_04020"
     CDS             complement(3502..3888)
                     /locus_tag="VF225_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7144453.1"
                     /translation="MSRPFVLIVDDDEVIRSLLRLTLPDEGYDLVEAEDGDQALQIIA
                     ARQPSLVLLDWRMPGTSGEGVLTALKQTYPEVPVIVLTAEPEANVRTLAESLGADVFV
                     AKPFSPLQLLGSVERLLPERPLDEAS"
     gene            complement(3885..4097)
                     /locus_tag="VF225_04025"
     CDS             complement(3885..4097)
                     /locus_tag="VF225_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7144454.1"
                     /translation="MSERHGIPDEILSALAHDLRTPISVIVGYAELIERRSEEVKTRE
                     AAANILEAAERLRSAVDRLLRDARAQ"
     gene            complement(4094..4726)
                     /locus_tag="VF225_04030"
     CDS             complement(4094..4726)
                     /locus_tag="VF225_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144455.1"
                     /translation="MTVPRLRLAPDSPLSVLLVEDDAMVSGWVRMAFENTEIRLAGVA
                     VSAAEALALASRRQLDVLLLDYRLPDGRGTELVRDLRLNGVSAPALLMTANEENGFNE
                     AAREAGAQGTVLKTGSADELVEAIRRVHGGEAAYDARHPRRAAGKTALSPREREVLRL
                     VASGSTNQEIAEQLGVGSETVKTLLARTFSKLGVRRRAEAVSAAHDLGLL"
     gene            complement(4723..5409)
                     /locus_tag="VF225_04035"
     CDS             complement(4723..5409)
                     /locus_tag="VF225_04035"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7144456.1"
                     /translation="MADDLAAAQALRYAEELRSLYAEERKQRRTAERALEQLGDSYST
                     TVRALAAALELRDDETGGHAERVTRLALELARSIDPDLLDDPQLEYGFLLHDLGKIGV
                     PDSILLKPGPLTARERETMRDHPILGERIVARVPYLRGTARQIVAAHHERWDGGGYPR
                     RLRALDIPRCARIFAVVDAFDAMTNERPYRAAMKIDLALAEIERCRESQFDPLVVEAF
                     LSLQDIRPAA"
     gene            5665..7026
                     /locus_tag="VF225_04040"
     CDS             5665..7026
                     /locus_tag="VF225_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7144457.1"
                     /translation="MAFRRGLALRGTTLAIVATVSLIALGLVVMAVSYRHAVADHQLV
                     ARAHDRDKLAQRSEKYFWREREAMNEFLLRPTPETLREIDSLGAQFERVLAELQGVST
                     KQQARDVLAAAEAHASFVQEFHADRPAAGRGVVPENAVGDGLKAMEPRVLVPLSRIGP
                     SDDRDERNQAAAAKADGRRALTAGILAALLAIGGGAAFAVYAVRLLRRIRAQNEQLRT
                     FDKLKDDFVASVSHELRTPLTSIRGYLELLISGEAGELNDEQERFLAVVDRNADRLLS
                     VVGDLLFVSQVEAGVISLDRHSFELVGLLKESVDAARPAAAAKGLVVELSADDVAEVD
                     ADRGRLAQVFDNLLSNAIKFTPSGGTVDVRISAEGEQAKIRVEDTGMGISADDQMRLF
                     TRFFRAPAANELAIQGTGLGLAIVKAIVESHGGRITVESELGRGTTFCIELPLAVREV
                     AAA"
     gene            7127..8482
                     /locus_tag="VF225_04045"
     CDS             7127..8482
                     /locus_tag="VF225_04045"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX7144458.1"
                     /translation="MSTRKVLALDVGTSSVRARVFDETAEERDEPARRDYPGEYDADR
                     VLARTREVIEEVGGAAEVDAVGAACFGHSLVALDRRGRPLTPILSWRDTRSADAADRL
                     LRRLDNVAVHARTGCQIHTSYWPAKLAWLAEERPEVFRSAHRFVSFCDYLYERLLGRE
                     IPTSISMASPTGLVDLRTRTWDSELLETLDLAAERLPVISDAPVDSWYPALFDGACSN
                     LGAGCVTRERAALMVGTSGAIRVVYETDRPEPRTGLFLHWIDETRVVEGGSLSDGGNL
                     YAWMEQTFREAGAKSLAGRDPDSHGLTFLSLLGGERSPGWHQHAKGAVHGLTFATTPL
                     DLRQAGLEGVAFRFAQVAELIPGVEEIVVTGGALLKDPDWVQIMADALGRPVTTSGVE
                     EASLRGAAVVVLERLGETPPPAPLGPVVEPRPDKVEVFRAARERQRALYEVVTSEPTS
                     S"
     gene            complement(8446..10293)
                     /locus_tag="VF225_04050"
     CDS             complement(8446..10293)
                     /locus_tag="VF225_04050"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7144459.1"
                     /translation="MDANELRELGQQFRVDSIRPAAVAKSGHPTSGMSAADLMAVLVA
                     NHLHYDYDKPKRPANDRLIFSKGHASTLYYAILRAAGAITDEELLTYRKFGSMLEGHP
                     TPLIPWVDVATGSLGQGLPIGVGMGLAAKYLDRLPSRVWVLCGDSEMAEGSMWEAFEH
                     ASHYELDHLTAIIDVNRLGQRGETMIGWNTGVYVERARAFGWNAIEIDGHDVEEIDRA
                     YKEAEETAGRPTVIVARTIKGKGVKAVEDKDGWHGKPLDDPDAAIKELGGIRNIHVDV
                     AKPEPADPHRFPDGPLELPRYELGQEVATRKAYGEALAALGSARGDVVALDGEVSNST
                     FAEIFKQAHPDRFFEMYIAEQQLVAAAVGLHARGWNAFGSTFAAFFSRAYDFIRMAAI
                     SRVDLRLAGSHAGVSIGEDGPSQMALEDIASLRAIHSSVVLHPCDANQTAKLVVAMAD
                     TKGIVFLRTLRPNTPVIYGPDEEFEVGGSRVIRSSDDDDVTIVATGITVHEALKAAEA
                     LEEDGITARVIDAYSIKPIDAEALQAAAEATGKIVTVEDHFPEGGLGDAVLAALAEKD
                     EHPRVLKLAVREMPRSGKPEQLMNAYGIDAEHIETAARQLLVGSEVTTS"
     gene            10196..10804
                     /locus_tag="VF225_04055"
     CDS             10196..10804
                     /locus_tag="VF225_04055"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX7144460.1"
                     /translation="MPEVGCPDFATAAGRIESTRNCWPSSRSSFASIEAILGGRARAL
                     SPVGYGPDVKVAVAFDHRGVHLRDAVLDVLAGHSLVDLGTDTDKVRIDYPEKARLIGE
                     TILRGDAERGVFVCGSGVGASIAACKLPGIRAAICHDVYSAHQGVEHDNMNILCLGSE
                     VVGPSLTRDLVTTFLRARFDGGERYVKRLKQIEDLERNASHV"
     gene            10797..11906
                     /gene="tal"
                     /locus_tag="VF225_04060"
     CDS             10797..11906
                     /gene="tal"
                     /locus_tag="VF225_04060"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7144461.1"
                     /translation="MSESNLHKLSALGQSVWIDYLSRQMLQSGKLAKMMEEDAVVGVT
                     SNPTIFQKAISEGDAYDKQLKEILDSGEEDPKEIFLQLSSHDISDACDLLRAVWDEGG
                     GLDGYVSWEVDPTLAYDRDGTIAEAHRLHDWIDKPNLYVKIPATEPGLGAIEEMIASG
                     KSINVTLIFSLERHKEVMEAYIRGLERLVESGGDPNEVHSVASFFVSRVDTETDRRLD
                     EIGYATKLKGKLAIANAKLAYQNYLEIFAGPRWEALEAKGATKQRCLWASTSTKNPDY
                     RDVMYVEELIGPETVNTMPEETIRAFQDHGKVELTLAKDVDAARKLFKDLAAAGISYD
                     DVSDTLEREGVQKFSDSFEELLEGIRAKRGALATA"
     gene            11903..13258
                     /locus_tag="VF225_04065"
     CDS             11903..13258
                     /locus_tag="VF225_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144462.1"
                     /translation="MTVLTQEKLVERIWARDPTVWTGADEDKWLGWLDEPIRMQERTG
                     EIAQFAAAARTKFETFVLLGMGGSSLAPEVLKRTFGAEQLHVLDTTHPAMIRHAERSL
                     DLSKTMFVVSSKSGGTLETLSHFEYFWEKAGKSGEQFVAVTDPGSALEELATERGMSV
                     FAGEPTIGGRYSALSPFGIVPAALMGIDIERVLASGERMAEACRGDANPGLQLGLQLG
                     EHWREGRDKVCIGETDGNFGLWAEQLIAESTGKQGKGLVPAPGESPDGPDRQAAEVQL
                     ADPDEIGAEFFRWEFAVAVAGSILEINPFDQPDVQAAKDKTKEVLASGEDPQLEPEGS
                     LDELLEGAEPPNYIAIQAFIDPLRERELEPLIERGHETACVVTHGLGPRYLHSTGQLH
                     KGGPPTGLFVQVVDDTGEEIPIPGQDFGFGRLIRAQAEGDFRSLRERGRPIVRVRLED
                     L"
     gene            13258..14160
                     /gene="gnd"
                     /locus_tag="VF225_04070"
     CDS             13258..14160
                     /gene="gnd"
                     /locus_tag="VF225_04070"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7144463.1"
                     /translation="MQLGMIGLGRMGSGMTERLAEHGHELHTYDPNVATRTADSLERL
                     KEQLKAPRAFWMMIPAGEITEQTFHQLLEIADEGDTIVDGGNSNFRDSKRRYVEASHR
                     GIHFVDAGVSGGVWGLEVGFCLMVGGDEEPVKRLEPVFKALAPEDGYAHVGPSGAGHF
                     VKMVHNGIEYGLMQSYAEGFEIMEHSEYDLDAYEISGIWRNGSVVRSWLLELLHAAFE
                     THGSKLEDIAPYVEDSGEGRWTINEAINENVPAPVIAASLFARFQSRDEIKFSARVAA
                     ALRNQFGGHAVRAVERAKAGSDPR"
     gene            14163..15671
                     /gene="zwf"
                     /locus_tag="VF225_04075"
     CDS             14163..15671
                     /gene="zwf"
                     /locus_tag="VF225_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7144464.1"
                     /translation="MAIAQENPLLEGLELRRAPDNCILVIFGASGDLTKRKLFPALYS
                     LAFRRLLPEHFAVVGVARSPETDDEFRERMKEAVQEHSRDEFRDDAWELLASGMRYVA
                     MDFADEEREDGLAKTLTELDEERGTGGNRVYYLATPPAAFETVVRAVGKRRSAQGWVR
                     LIIEKPFGHELASARELQKTIEEYFEEREVFRIDHYLGKETVQNMLALRFANGIFEPI
                     WNRQFVDHVEITVAETMGIEGRAGYYEQAGAVRDIFQNHMLQLIAITAMEPPIDFTAD
                     SVRTEKVKVLRAMHTPGPKSVVRGQYGRGYIEGEDVPAYREEQGVDPNSMTETYVAAK
                     LYVDNWRWADTPFYVRTGKRLARRETTIAIQFKRAPHPPFEELSAERLRPNVLIVHVQ
                     PDEGVSLAIGVKVPGQGMTIRTVHMDFLYGGAFRTGLPEAYERLILDAMLGDATLFTR
                     IDEVEEQWALVDAIVAAWARDRPAFPNYAAGTWGPTAADELMERDGRSWRLR"
     gene            15709..16560
                     /locus_tag="VF225_04080"
     CDS             15709..16560
                     /locus_tag="VF225_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX7144465.1"
                     /translation="MATALGSIREIERQVAALRIAPGSDVPYQRTSVMTHTAWVPPEW
                     VEAADDVLAGLAERHPSRTIVLVPEPEREDGLEAQVDVEIFPSGEGRQICAETIRIRL
                     CGPRAAAPASVVQPLFLPDLPVFLRWRGVPSFGSDSFESLVGVVDRLIVDSTEWNELP
                     VAYAQLAGVFDRVVVSDIAWARTSRWRRQLASLWPGIGNVERIRVTGTAAQAELLAGW
                     LRSRLSRPVQLEHEPADHLEAVEIDGKPAPFPPGDAPPPSDLLSEELDRFERDRIYEE
                     AVRSAAG"
     gene            16557..17261
                     /locus_tag="VF225_04085"
     CDS             16557..17261
                     /locus_tag="VF225_04085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144466.1"
                     /translation="MNDFTVHVKPLIAEVPGWLTDEEGEALYDLARACTGKGVIVEIG
                     SWKGKSTICLGRGSLAGVSVPIYAIDPHADYRFGDFKANVDRAGITELVRPVTSLSQP
                     AADEFHEPIELLFVDGSHEYDLVLEDFEKWVPKVVEGGWVAFHDTTWTSGPRKVVGQA
                     IYRSNRFKDARFVVGSTTVARKVDQNSFADRARNRYVLGVKTAFWLGSTVVKKQRRLL
                     PKPVERIGRRFLKAIQ"
     gene            complement(17381..19339)
                     /locus_tag="VF225_04090"
     CDS             complement(17381..19339)
                     /locus_tag="VF225_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144467.1"
                     /translation="MRTPLLAAVCLGLLLLAPAGEAATAVRGAPPTALHGFLLRADEP
                     VRNSFPRTPSFAWNPAPGALRYQFQLATSSSFRESGIVYSTTGLTTPVAAPTVMLPWI
                     TGNPHALFARVRGITASSTTPWSASFGFDMEPGAAPKPLPSYPGLLRWTPIEGADGYE
                     VWFVDMPKIETVFTNVLDERELYTFHRTSNWTSSVRWRIRALRTDRTDNEKKTRQNGL
                     PAVGYGPWSGVYNSTNPAYQGGPITLGSTVSDIVSTGDAGSDSHRLMPAFTFTGDQTL
                     DGTSAELFRIYVFTDRQCLNRVYTSAVIGGPAYAPRPFGPLALPSVTAAMAAARASYL
                     PDGSEATGYTVDGLQVKTTEAAAPAKPTTAVPSDSDTNPGGSGPSPPAGPEQIEVGAG
                     VLGAPVDLWDTDWPSSGYYWTVIPVAALSPGALSTSTAATAALGVSSVTVASTLGFST
                     GDTVLLGNKANQEPVTVIAVSGTTLTLAAPLKFGHGVGEPLVRSGGSLQYRDLELPQD
                     VCAPPYNRVARFGKQSEPSLTAAGELFASGLSPKGRLTAGTSSQAFYGSPLVAWTPAL
                     GATVYEVQWSKKRYPFKPLPNPQNQSALGTLTLGTSYVLPLKPGTWYYRVRGFNYTLP
                     TGAQQMSWSDPAKIVVAKPKFRVIGGGK"
     gene            complement(19416..>20956)
                     /locus_tag="VF225_04095"
     CDS             complement(19416..>20956)
                     /locus_tag="VF225_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144468.1"
                     /translation="NREDHSTVCPTMFITQEQAGDVLGMAERAYALYASWGYATPILD
                     ISDGDGYLDISADQFSLPPNGCIAHGAIDLSIPREVDGTLGRWDALINPVLPLGAGEI
                     HLDTVKGLTYHVIAHEVFHLFQGKITTSADPWVAEGTAEWAAVKADGAAGGLEVNPDR
                     TLDCVGAECGDAEFDKNGYPGWMLFEYLTEHYGDAKIKDILSAAPMTGTQALASVLPA
                     PVKLQDFFNDYTTARLNGDFSSPALAGVLPQSLATLSLGNASGLFSVTPIAVNHLAVR
                     YLELAHGDDTDTGPCYVASLKLEVRIPANVVSKPYYYANVAGSVPQALTVSGSTASID
                     HVPWDTCSGSPHAYLSLPNDWNDADQVGPPKDGREFVVTGFLTVDKSKPASAASPPPG
                     VKVIGPVIQVPTTDPAPDLSVYAPELLRVSAKTHVLRFVVFSSGTGALRAVLGSSWNG
                     SATLHAGNNDIRFVLPTQLLKRLRKTSSENILSLTSYSPGGARGETITRRVAVQTTKP
                     KRRK"
BASE COUNT         3503 a         6717 c         7201 g         3535 t
ORIGIN      
        1 ctgctcgtcc ttctgccggt cttcggcctg cgcgccttct cggcgatcga cgaccgccgg
       61 gctgcgattc gcggcgcgga gggtgacgcc tacgacacgg cgcgcatcgt ggcgcaggat
      121 cagcaagggc tgctcgagca gtcgcggcaa cagctgttca ccttcgtcca actcgttgcc
      181 ctcagttccg ggcggccgat cgagccccgc acctgcaagc cggccctggt caccttcatg
      241 aagcgtctac caaactacgt cgacctcggc atcgtcgctc cggacggcaa ggttctctgc
      301 gcggcgacca cgaaagcggg agccgacttc gcgcgcgaac cgttcgtccg ccgcgctctg
      361 cagagtcggc agttcgcaat cggcgactac gagttgcgcc ccgcggtcgg caagccgacg
      421 atcagtttgg cgtatccagt tgtggtgcga ggccgcgtct cgtcgatcat gttcgcgacg
      481 ctcgacctcg ccgcgctcaa ccgccgggcc gccgagaccc cgctcgctcg cggctgggta
      541 ctgaacatca tcgacaaccg cggaaccatc ctcgtgcgtc agccgagctc cagccagtgg
      601 ataggtcgca cggtccgtga cgcgccgctg atgcgcgcag tgctgcgcga aggcaaaggc
      661 agggtcaagg ctcggggcgt tgacggcgtc acccgcttct acggcttcac agggctcttc
      721 gggctggaga gcgggagcga tctccatgtc accgtcggca tacccaagga cctcgcgctc
      781 gcagcctcga cacgcaaggc caagcgcgac ttcgccctgc tcgcgctcgt cgcactcgcc
      841 acccttctga tcgcctggtt gagcagccga atgttcttcc tgcggccgct cgacgcgctg
      901 acccgcacgg ctcagcgtct ccgggccggt gatctaggtg cgagaaccgg gctccgtgag
      961 cgggcgggcg agctcggtga gttggctcga accgtcgacg agatggcgga atcgctcgaa
     1021 ttgcgagagc gcgagatcaa tcggctcaac gaggatctcg agcgccgcgt cgccgatcgg
     1081 acggtagagc tcgaggcggc tcgggacgag ctcgaaagcc agaacgcaga gctggagctc
     1141 cagactgtgg agctcgagga ccatcagacc gagctggcgt ccgccaacga tgagctggag
     1201 gcacagcggg ccgagctcga ggtgctactg gaggacctcg cgcacgagaa ggagcggctg
     1261 gagctcctgt atgccttcgg ggagatgctc gcgcgcgaga ccgagagcgg tgggcttgcg
     1321 gacaacgtca tgcgtgagct cggcgacctc gccgaggcag aagtgggcgt gctgtacgtc
     1381 gccgcaccgg agaaggacta tgagctcgtt ctggcgacca cccgcgggat cgaccacgca
     1441 ggactacctg agcgaatcac accgggcaag ggcctcgccg ggcgtgcggt cgacgagctc
     1501 aggcgaatcg tcgtcgcgcg cggtgactcc gagttgcgag tagagagttt tggcaaggaa
     1561 gtcgaggtca ggcacgaggt gcacctgccg ctctcgtcgg ccgggcgcac gctcggggtg
     1621 atcactcttg ggcggctcgg tgaccggccc ttcaccgagg agcaactcga gacgctcgac
     1681 catctcgccg gccagaccgc cgttgcgatc tccaacaagc ttgccttccg cgaggccttg
     1741 cggctcggag agatcactca ggcggttatc gaggccactc aggacggaat cgctctcgca
     1801 aatcaagagg gcgaaacgat cctcatgaac gccaggctga agcacatgtt gagcgacctc
     1861 ttggaactcc caccaggccg caccgtgcag gagtacatgg aaatcatgcc ggagcgcgtg
     1921 acggagccgg agcgctaccg ggaggtcacg gcggccatcg cgcgcgatcc ggagtacgaa
     1981 ggtgtcactg agttcgagct ccgctcgggc cgttccttca cgcgctacac cgcaaccgtg
     2041 cgaggcgccg acggccaaca gatcggccgg atcttcgtcg tccgcgagac gactgcggac
     2101 cgtgaggtcg aacgtctcaa gtcggagctc gtggcgaccg tgtcgcacga gttgcgaaca
     2161 ccactctcga gcatcatggg cttcgccgag ctgctcgcgg accgtgatta cgacgaagcg
     2221 acacgcaagc ggttcctggc gaccatccat ggtgaggccg cccgcctgac tgcgctcgtc
     2281 aacgagttcc tcgatctcca gcggatcgaa gacggcagct tcacactggc gctcgaggcg
     2341 ttcgacctcg acgcgctcgt gcgtgaccaa gtcgagctct tctcagcgca gagcgacgcg
     2401 cacacgttgg acctcgagct tcccgacggg gccgtgccgg tcgtgggtga gcgtgaccgc
     2461 atcaaacagg tactcggcaa tctgctctcc aatgcgatca agtactcgcc ccacggcggc
     2521 gtcgtccagg tgcggctcca gccgaacggc acggctgtgc ggctctccgt cagcgacgaa
     2581 gggttgggca ttccaggcac acagcagggt ggcgtcttct cgaagttctt ccgcgccgac
     2641 tcctccgaca cgcgcgaaat cggaggcacc gggctcggcc tcgccctctg caaggacatc
     2701 gtcgaggcgc atgccgggca gatcgggttc acgagcgtgg aaggcaaggg ctcgaccttc
     2761 tggttcgaac tgccgagccc gagctcgtcg aacgggcgac gcgacgatcg tccggtcgtc
     2821 ctcgtggtcg aggacgatcc cgaggcagcc tctctgctgt cgcactatct gctggaggcg
     2881 gactacgcgg tcgtcactac cgtgacgggc gaagaggcgc tcgcgcacat tgcgcagcat
     2941 cgtccggacg ccatctgcct cgacatgact cttgccggtg agctgagtgg ctgggatgtg
     3001 ctctctcggt tgaaagagag ccctgcgacg agcggtatac ctgtggtcat ctgcaccgcc
     3061 ggaaacggac gcgccgacgc ctccgcgctc ggtgcggccg acttcctcgc caagccgttc
     3121 gcggccggcg agctgcacgc cgcgcttcag cgcgtcctcc caacgggtcg aggttccgtg
     3181 ctgatcgtcg acgacgagga gagcgtccgg gcgctggtcc tcgagacgct gtccggaacc
     3241 ggttacgagc tgcgggaggc ggcggacggc gaagaggccc tcgagcggat tgcggcgcgt
     3301 cgtccggatg cgatcgtcct cgacctgacg atgccgaagc tcgacggctt cgccgtgctc
     3361 gaacgccttc agggagaccc cgagacgcga cggatccccg tcgtcgtcct gaccgctcgc
     3421 gctctgctgg ctgcggagcg atccttgctc cagcgccgcg ccgtcaactt gctcgagaag
     3481 aacgactact cgggcgagga gctacgaggc ctcgtccagc gggcgctcgg gtagcaaccg
     3541 ctctacggat ccaagcagct gcaacggact gaatggcttg gccacgaaga catcggcgcc
     3601 gagcgactcg gccaacgtgc ggacgttcgc ttccggctcg gcagtgagga cgatcaccgg
     3661 aacctcggga tacgtctgct tcagagcagt gagaacgcct tcacccgagg tcccgggcat
     3721 tctccaatcc aggagcacga gcgagggttg gcgtgcggcg atgatctgca gcgcctgatc
     3781 tccgtcctcg gcctcaacca ggtcgtaccc ctcgtcaggc agtgtcaagc gcagcaggct
     3841 gcgaatcacc tcatcgtcat cgacgatcag gacgaacggc cggctcactg cgcccttgcg
     3901 tcgcgcagca accggtcgac cgcggagcga agtcgttctg ccgcctcgag gatgttggcc
     3961 gcggcttcgc gcgttttgac ctcttccgat cgtcgctcga tcagctcggc atagcccacg
     4021 atgaccgaga tcggagtcct gagatcatgt gccagcgccg aaaggatttc gtcgggtatc
     4081 ccgtggcgtt cgctcacagg agcccgagat cgtgggcggc tgagacggcc tcggcgcgac
     4141 gccgcactcc cagtttcgag aaggtgcgcg cgagcagcgt cttgacagtc tctgagccga
     4201 ccccgagttg ctcggcgatc tcctgattcg tggagccgga agcgacgagt cgaagaacct
     4261 cccgctcgcg cgggctgagg gccgtcttcc cagcggcacg acgggggtga cgtgcgtcgt
     4321 aggcggcttc gccgccgtgc acgcggcgga tggcttccac gagctcgtcg gcactaccgg
     4381 ttttgagcac cgttccctgc gctccggctt cgcgtgcagc ttcgttgaag ccgttttcct
     4441 cgttggcggt catcagcaat gcaggtgcag atacgccgtt gaggcgaagg tcgcggacga
     4501 gctctgtgcc ccgcccgtcg ggaaggcgat agtcgaggag cagcacgtcc agctgtcttc
     4561 ggctcgcgag tgcgagtgct tctgctgcgg acacggcgac gccggcgagc cggatctcgg
     4621 tgttttcgaa agccatacga acccagcctg acaccatcgc gtcgtcttcg acgagcagaa
     4681 cggagagcgg cgagtcggga gcgagccgaa ggcgagggac agtcatgcgg ctggcctgat
     4741 gtcctgcaat gacagaaacg cctccacgac gagcgggtcg aactggctct ctcggcagcg
     4801 ctcgatctcg gccagcgcca gatcgatctt catggcggcg cgatagggcc gttcgttcgt
     4861 catggcatcg aaggcgtcga cgacggcgaa gatccgagcg caccgaggaa tgtcgagggc
     4921 tcgcaggcgg cgcgggtagc cgcctccgtc ccagcgctcg tgatgagccg ccacgatctg
     4981 tcgcgcggtg ccgcggaggt aggggacgcg ggcgacgatg cgctcgccga gaatcggatg
     5041 gtcccgcatc gtctctcgct cgcgcgccgt cagcgggccc ggcttgagca agatcgaatc
     5101 cgggacgccg atcttgccga gatcgtgcag caggaacccg tattccaact ggggatcgtc
     5161 gaggaggtcg gggtcgatcg acctggcaag ctcgagtgcg agccgtgtga ctcgctcggc
     5221 gtggccgcct gtctcgtcgt cgcgtaactc gagcgctgcg gcgagtgccc tcacggtcgt
     5281 cgagtacgag tcacccagtt gctcgagtgc gcgctccgcg gtgcgtctct gctttctctc
     5341 ctccgcgtag agactgcgta gctcttccgc gtacctcagc gcctgcgcgg ccgccaggtc
     5401 gtctgccaca gatcgtcctc tcttgtcaaa tcgagtgggc aagctgccca tttgtaagcg
     5461 ttccgtcctg acttcgacag tcggttgggc aaactttgtc ccccgaacgg gggacaaaag
     5521 cgcttccacg caagttttgc cggtatccag cgctctcatc cggcgggcaa agaaacggaa
     5581 aggcgcagcg tctgagctgc aaggcaggga atcccccgaa agggggccac catcgaggcg
     5641 gactcccccg atctagtgaa ccggatggca tttcgccgag gtctcgccct gcgtggaaca
     5701 acgctcgcga tcgtggccac agtgagcttg attgcactgg gcctcgtggt catggccgtg
     5761 agctaccgcc atgctgtcgc ggaccatcaa cttgtcgctc gagcccatga tcgcgacaag
     5821 cttgcccagc gctctgagaa gtacttctgg cgcgaacggg aggcgatgaa cgaattcttg
     5881 ctcaggccga ctccggaaac tcttcgcgag atcgacagct tgggcgcgca attcgagcgc
     5941 gtgctcgccg agttgcaagg agtctcaacg aagcaacagg ctcgagacgt tcttgccgcc
     6001 gccgaggcgc acgcgtcgtt cgttcaggag tttcatgcgg atcgaccggc cgcaggccgg
     6061 ggcgtcgttc cggagaacgc ggtcggtgat ggtctgaaag ccatggaacc gagggtcctc
     6121 gttccgcttt cccggatcgg ccccagcgac gatcgagacg aacgcaacca ggccgcggcc
     6181 gcaaaagccg acggtcggcg tgcgctcacg gccggcattc tcgcggctct acttgcgatc
     6241 ggcggcggcg cggcattcgc cgtctacgcg gtccggctcc ttcgacggat ccgggcccag
     6301 aacgagcaac tgcggacctt cgacaagctg aaggacgact tcgttgcgtc ggtttcacac
     6361 gagctgcgaa cgccgctgac ttcgattcgc ggctatctgg agctactcat cagcggtgag
     6421 gccggcgagc tgaacgacga gcaagaacgc tttctggccg tcgtcgaccg caatgcggac
     6481 aggcttctca gcgtcgtcgg cgatctcctc ttcgtctcgc aggtcgaggc gggtgtgatc
     6541 tctctcgatc gccactcgtt cgagctcgta ggactcctga aggagtccgt cgacgcggca
     6601 cggccggctg cagcggcaaa gggcctggtc gtcgaactga gcgcggatga cgttgcggaa
     6661 gtggacgccg accgcggccg gctggcgcag gtcttcgaca acctgctctc gaacgcgatc
     6721 aaattcaccc cgtcgggtgg aacggtcgac gtgcgaatct ctgcggaagg cgagcaggcc
     6781 aagatccggg tcgaggacac tgggatgggg atctcggccg acgaccagat gcgtttgttc
     6841 acgcgtttct tccgggcgcc cgctgcaaac gagctggcga ttcaggggac gggtctgggc
     6901 ctcgcgatcg tgaaggcgat cgtcgaaagc cacggcggga ggatcacggt cgaaagtgag
     6961 ctggggcgag ggacgacctt ctgtatcgag ctaccgctcg ccgtcaggga ggtcgcggcc
     7021 gcctgatcgt cacatcacga ggcgatggga gttggggcga gatgcagcgg cgtgagctcg
     7081 gcccgaggaa ggaggcggcg agccgctcgt agttatcgtc cggccggtgt ccacgcggaa
     7141 ggttctcgct ctcgatgtcg gcacctcgtc ggtgcgggcg cgcgtgttcg acgagacggc
     7201 cgaggagcgg gacgagcccg cgcgacgcga ctatcccggg gagtacgacg cggaccgagt
     7261 tctcgcgcgc acgcgcgagg tgatcgagga ggtgggcggc gcagcggaag tcgacgcggt
     7321 cggcgcggcc tgcttcggcc acagtctggt tgcgctcgac cggcgcggcc ggcccttgac
     7381 gccgatcctc tcctggcggg acacgcgttc cgccgacgca gccgaccggc tcttgcggcg
     7441 cctcgacaac gtcgcagtcc atgccaggac gggctgccag atccacacga gctactggcc
     7501 ggcgaagctg gcgtggctcg ccgaggagcg gccggaggtg tttcgctccg cgcaccgctt
     7561 cgtctccttc tgcgactacc tctacgagcg gctgctcggc cgtgagattc ccacgagcat
     7621 ctcgatggcg tcgccgaccg ggctcgttga cctccgcacg cgaacgtggg actcggagct
     7681 gctcgagacg ctggacctcg ccgccgaacg cttgccggtg atctcggatg caccggtgga
     7741 cagctggtac ccggcgctgt tcgacggcgc ttgctccaac cttggagcgg gttgcgtgac
     7801 ccgtgagcgt gcggccctga tggtcggtac gtcgggtgcg atccgggttg tctacgaaac
     7861 ggacagaccg gagccgcgca ccgggctctt tcttcactgg atcgacgaga cacgtgtcgt
     7921 cgaaggcggc tcgctctcgg acggcgggaa tctctacgcc tggatggagc agacgttccg
     7981 agaggccggc gcgaagtcac tcgcaggccg cgatccggat tcgcacgggc tgacgttcct
     8041 gtcgctgctc ggcggtgagc gcagtccggg ctggcaccag cacgccaagg gcgccgttca
     8101 cggactgacc tttgccacga cgccgctgga tctgcgccag gccggcctcg aaggcgtcgc
     8161 gttccgcttc gcgcaggttg ccgagttgat cccgggagtc gaggaaatcg ttgtcacggg
     8221 cggcgcactg ctcaaggacc ccgactgggt gcagatcatg gcggacgcgc tcggccggcc
     8281 ggtcacgacg tccggggtcg aggaggcgtc gctgcgcggt gcggccgtcg tggtgctcga
     8341 gcggctgggc gagacgccgc ctccggcgcc actcggcccg gtggtcgagc ctcgcccaga
     8401 caaggtcgaa gtattccgtg cggctcgcga gcggcagcgc gcgctttacg aggtggtgac
     8461 ttccgaaccg accagcagct gacgtgccgc cgtttcgatg tgctcggcgt cgatgccgta
     8521 ggcgttcatg agctgctccg gcttgcccga gcgcggcatc tcccgcaccg ccagtttcag
     8581 cactcgcggg tgctcgtcct tctcggccag cgcggcgaga acggcgtcgc cgaggccgcc
     8641 ctcagggaag tggtcttcta cggtgacgat cttgcccgtg gcctcggcgg ctgcctgcag
     8701 cgcctcggcg tcgatcggct tgatcgagta cgcatcgatc acacgcgcgg tgattccgtc
     8761 ctcctcgagc gcttcggccg ctttcagggc ctcgtgcacc gtgatgcccg tggcgacgat
     8821 ggtgacgtcg tcgtcgtccg acgagcggat cacgcgactg ccgccgacct cgaactcctc
     8881 gtcgggcccg tagatcacgg gagtgttggg gcgcaacgtg cgcaggaaaa cgatgccctt
     8941 cgtgtccgcc atcgccacca cgagcttggc ggtctgattc gcgtcgcagg gatgcagcac
     9001 cacgcttgag tgaatcgcac gcagcgacgc gatgtcctcg agcgccatct gggaagggcc
     9061 gtcctcgccg atcgacacgc ctgcgtgcga gcccgccaga cgcaggtcga cgcggctgat
     9121 ggccgccatg cggatgaagt cgtaggcgcg tgagaaaaat gccgcgaagg tcgagccaaa
     9181 tgcgttccag ccgcgcgcgt gcaggccgac tgccgcggcg acgagctgct gctccgcgat
     9241 gtacatctcg aagaagcggt cggggtgcgc ctgcttgaag atctcagcga acgtcgagtt
     9301 gctcacctcg ccgtcgagcg cgaccacgtc tccgcgggcg gacccgaggg ccgcgagcgc
     9361 ctcgccgtac gccttgcgcg tcgccacttc ctggcccagc tcgtagcgcg gcagctcgag
     9421 cggtccgtcg gggaagcggt gcgggtcggc aggctcgggc tttgcgacgt cgacgtggat
     9481 gttccggatg ccgccgagct ctttgatcgc cgcgtcggga tcgtcgagcg gcttgccgtg
     9541 ccagccgtcc ttgtcctcga cggccttgac gcccttgcct ttgatcgtgc gcgccacgat
     9601 caccgtcggc cggccggccg tctcctctgc ttccttgtac gcgcggtcga tctcctcgac
     9661 atcgtggccg tcgatctcga tcgcgttcca gccgaacgct cgcgcgcgtt cgacgtagac
     9721 gccggtgttc cagccgatca tcgtctcgcc gcgctgcccg agccggttga cgtcgatgat
     9781 cgcggtcagg tggtcgagct cgtagtgcga cgcgtgctcg aaggcctccc acatcgaccc
     9841 ctcggccatc tcgctgtcgc cgcagagcac ccagacacgg gagggaaggc ggtcgaggta
     9901 cttggctgcg agacccatgc cgaccccgat cggcagtccc tgcccgagcg agcccgtcgc
     9961 cacgtccacc cacgggatca agggagtcgg gtgcccctcg agcatactgc cgaacttgcg
    10021 gtaggtcagc agctcctcgt cggtgatcgc cccggcggcg cgcagaatcg cgtagtacag
    10081 cgtggaggcg tgccccttcg agaagatcag ccggtcgttc gccggccgct tcggcttgtc
    10141 gtagtcgtaa tggaggtggt tcgcgacgag gacggccatc aggtcggcgg cggacatgcc
    10201 cgaggtggga tgccctgact tcgcgacggc cgccgggcga atcgaatcga cgcggaattg
    10261 ctggccgagc tcgcggagtt cgttcgcgtc catcgaggcc attctaggag gcagggcgcg
    10321 tgctctgtcg ccggtggggt acggtccaga cgtgaaggtg gcggttgcat tcgaccaccg
    10381 tggcgtccat ctccgcgacg cggtgctcga cgtgctcgcc gggcactcgc tggtggacct
    10441 gggcaccgac accgacaagg tgcggatcga ctacccggag aaggcccgcc tgatcggcga
    10501 gaccatcctt cgcggcgacg cagagcgcgg cgtttttgtc tgcggctccg gcgttggcgc
    10561 ctcgatcgcc gcctgcaagc tgccgggaat tcgagccgcc atctgccatg acgtctactc
    10621 cgcccaccag ggcgtcgagc acgacaacat gaacatcctc tgtctgggtt cggaggtcgt
    10681 cgggccgtcg ctcacccggg atctcgtgac gacgtttctc cgggcacgtt tcgacggtgg
    10741 cgagcgttat gtcaaacggc tcaagcaaat cgaagatctt gaaaggaacg cctctcatgt
    10801 ctgaatcgaa cctgcacaag ctgtccgcgc tcggccagag cgtgtggatc gactacctct
    10861 cgcggcagat gctgcagagc ggcaagctcg cgaaaatgat ggaggaggat gccgtcgtag
    10921 gcgttacctc gaacccgacc atcttccaga aggcgatctc cgaaggagat gcgtacgaca
    10981 agcagctgaa ggaaatcctc gactccggtg aagaggaccc gaaggagatc ttcctgcagc
    11041 tctcgtcaca cgacatctcg gatgcctgtg acctgctgcg cgcggtctgg gacgagggcg
    11101 gaggcctcga cgggtacgtc tcgtgggagg tcgacccgac ccttgcctac gaccgtgacg
    11161 gcacgatcgc cgaagcgcat cggctgcatg actggatcga caagccgaac ctctacgtga
    11221 agattcctgc gaccgagcct ggactcggcg cgatcgagga gatgatcgcc tccgggaaga
    11281 gcatcaacgt cacgctgatc ttttcgctcg agcgacataa ggaggtcatg gaggcataca
    11341 tccgcggcct cgagcgactt gtcgagagcg gcggcgaccc gaacgaggtg cactctgtcg
    11401 cgagcttctt tgtctcgcgc gtcgacacgg agaccgacag gcggctcgac gagatcggct
    11461 atgcgacgaa attgaaaggc aagctcgcca tcgcgaacgc gaagctcgcg tatcagaact
    11521 atcttgagat cttcgccggg ccacgctggg aagccctcga ggcgaaaggc gcaacgaagc
    11581 agcgctgcct gtgggcgtcg acgtccacga agaatcccga ctaccgcgac gtcatgtacg
    11641 tcgaggagct gatcgggcct gagacggtga acacgatgcc cgaggagacc atccgcgcgt
    11701 tccaggacca cggcaaggtc gagctgacgt tggcgaagga cgtcgatgcc gcgcgcaagc
    11761 tgttcaaaga ccttgcggcc gccggcatct cctacgacga cgtctccgat acgctcgagc
    11821 gtgagggtgt gcagaagttc agcgactcct tcgaggagct gctcgaaggg atccgcgcca
    11881 agcgcggggc gctcgccaca gcgtgacagt cctgacgcag gagaagctcg tcgagcggat
    11941 ctgggcgcgc gaccccaccg tctggaccgg cgcggacgag gacaagtggc tcggctggct
    12001 cgacgagccg atccggatgc aggagcgcac cggcgagatt gcccagttcg cggcggcggc
    12061 gcgcacgaag ttcgagacgt tcgtcctgct cggcatgggc ggctcctcgc tcgcgcccga
    12121 ggtgttgaag cggacgttcg gagccgagca gctgcacgtt ctcgacacga cgcaccccgc
    12181 gatgatccga catgcagaga ggtcgctcga cttgagcaag acgatgttcg tcgtctcgtc
    12241 caagtcgggc ggcacactcg agacgctctc gcacttcgag tacttctggg agaaggcagg
    12301 taagagcggc gagcagttcg tcgctgtcac cgatccaggc tcggccctcg aggagctggc
    12361 gactgagcgc ggcatgagcg tgttcgcggg tgagccgacc atcggcggcc gctactcggc
    12421 gctgtctccg ttcggaatcg tgccggctgc attgatgggg atcgacatcg agcgcgtcct
    12481 cgcgagcggc gagcgcatgg cggaggcttg ccgcggcgat gccaaccccg gccttcagct
    12541 gggccttcag ctcggcgagc actggcgcga gggccgcgac aaggtctgca tcggggagac
    12601 ggacggcaac ttcgggctgt gggccgagca gctgatcgcc gagtccaccg ggaagcaggg
    12661 gaaggggctc gtgccggcgc cgggggagag cccggacggc cccgaccgcc aggccgccga
    12721 ggtgcaactt gccgatccgg acgagattgg tgcggagttc ttccgctggg agttcgccgt
    12781 tgcagtcgcc ggctccatcc tcgagatcaa tccgttcgac cagccggacg tgcaggcggc
    12841 gaaggacaag acgaaggagg tgctcgcgtc cggcgaggat ccgcagctcg agccggaagg
    12901 ctccctcgac gagctgctgg agggcgccga gccgccgaac tacatcgcga ttcaggcctt
    12961 catcgatccg ctgcgcgagc gcgagctcga gcctctgatc gagcgcggcc acgagaccgc
    13021 ctgcgtcgtg acccacggcc tcgggccgcg ctacctgcac tccacggggc agctccacaa
    13081 gggcggtccg ccgacggggc tcttcgtcca ggtcgtcgac gacaccggcg aggagatccc
    13141 catcccgggc caggacttcg gtttcggacg tttgatccgg gctcaggccg agggcgactt
    13201 tcggtcgcta cgggaacgcg ggcgcccgat cgtacgggtc agactggagg atctctaatg
    13261 cagctgggga tgatcgggct cgggcgcatg ggctcgggca tgacggagcg ccttgccgag
    13321 cacggccacg agctgcacac atacgacccc aatgtcgcca cgcggacggc cgactcgctc
    13381 gagcggctca aggaacagct gaaggccccg cgcgcctttt ggatgatgat tcctgcgggc
    13441 gagatcacgg agcagacctt ccaccagttg ctcgagatcg cggacgaggg cgacacgatc
    13501 gtcgacggcg gcaattcgaa cttccgggat tcgaagcggc gctacgtcga agcgagccat
    13561 cgcggcatcc acttcgtcga cgccggcgtc tccggtggtg tctggggcct cgaggtcggc
    13621 ttctgcctca tggtgggcgg cgacgaggag ccggtgaagc gactcgagcc ggtcttcaag
    13681 gcactcgcgc cggaggatgg gtacgcacac gtcggcccat ccggtgcagg ccacttcgtg
    13741 aagatggtgc acaacgggat cgagtacggc ttgatgcagt cgtacgcaga gggcttcgag
    13801 atcatggagc attccgagta cgacctggac gcctacgaga tctccggcat ctggcgcaac
    13861 gggtcggtcg tccggtcgtg gctgctcgag cttctgcacg ccgcgttcga gacgcacggc
    13921 tcgaagctcg aggacatcgc cccttacgtc gaggactcgg gtgaaggccg ctggacgatc
    13981 aacgaggcga tcaacgagaa cgtgccagcg ccggtgattg cggcatcgct cttcgcgcgc
    14041 ttccagtccc gtgacgagat caagttctcc gccagggtcg ccgctgctct gcggaaccag
    14101 ttcggcggcc acgccgtcag ggctgtcgag cgcgccaagg caggctcgga cccgcggtag
    14161 ccgtggcgat cgcgcaggaa aatccgctgc tcgaagggct ggagctccgg cgcgcgcccg
    14221 acaactgcat cctcgtcatc ttcggtgcgt ccggcgacct gacgaagcgc aagctctttc
    14281 cggcgctcta ttcgctcgcc ttccgccgct tgctgcccga gcattttgcg gtcgtcggcg
    14341 ttgcgcgctc gccagagacc gatgacgagt tccgggagcg gatgaaggag gccgtgcagg
    14401 agcattcacg cgacgagttc cgcgacgacg cgtgggagct gctggcgagc ggtatgcgct
    14461 acgtcgcgat ggacttcgcc gacgaggagc gcgaggacgg gcttgccaag acactgaccg
    14521 agctggacga agagcggggg acgggaggca accgcgtcta ctaccttgcg acgcccccgg
    14581 cagctttcga gacggttgtc cgagccgtcg gcaagcgtcg ttcggcgcag ggctgggtac
    14641 ggctcatcat cgagaagccg ttcggccacg agctcgcctc tgcacgcgag ctgcagaaga
    14701 ccatcgagga gtacttcgag gagcgcgagg tcttccggat cgaccattac ctcggcaagg
    14761 agaccgtgca gaacatgctt gctcttcggt ttgcgaacgg gatcttcgag ccgatctgga
    14821 acaggcagtt cgtcgaccat gtcgagatca ccgtggcgga gacgatgggc atcgaaggtc
    14881 gcgccggcta ctacgagcag gcgggcgcgg tgcgcgacat cttccagaac cacatgttgc
    14941 agctcatcgc gatcaccgcg atggagccgc cgatcgactt caccgcggac tcggtgcgca
    15001 ccgagaaggt gaaggtgttg cgcgcaatgc acacgccggg gccgaagtcg gttgtacgcg
    15061 ggcagtacgg tcgcggctac atcgaaggcg aggacgtgcc ggcctatcgc gaagagcagg
    15121 gagtcgatcc gaactcgatg acggagacct atgtcgcagc gaagctctac gtggacaact
    15181 ggcgctgggc ggacacgccc ttctatgtcc ggaccggcaa gcggctcgcg cgacgagaga
    15241 cgactatcgc aatccagttc aagcgtgcgc ctcacccgcc tttcgaggag ctctccgccg
    15301 agcggctgag gccgaatgtc ctcatcgtgc acgtgcaacc cgacgaaggc gtgtcgctgg
    15361 ccatcggagt caaggttccc gggcagggca tgacgatccg gaccgttcac atggacttcc
    15421 tctacggcgg ggccttccga acgggcttgc ccgaggcgta cgagcgcctg atcctcgatg
    15481 cgatgctcgg cgacgcgacg ctgttcacac gcatcgacga ggtggaagag caatgggcgc
    15541 tcgtcgacgc gatcgtcgcg gcctgggccc gcgaccgccc ggcgttcccg aactatgccg
    15601 cgggaacctg ggggccgacg gccgctgacg agctgatgga acgcgacggg cggtcctggc
    15661 gtctcaggtg agcattccga tcgctccggc acctccagag gaggctcggt ggcgacggca
    15721 ctagggtcga tccgggagat cgagcgccag gtggccgcgc tgcgcatcgc gccgggctct
    15781 gacgtgccct atcagcggac gagcgtgatg acccacacgg catgggttcc gcccgagtgg
    15841 gtcgaagcag cggacgatgt cctggccggc ctggcggagc gccatccctc tcggaccatc
    15901 gtcctcgtcc ccgagcccga gcgagaggac gggctcgagg cgcaggtcga cgtcgagatc
    15961 ttcccctcgg gcgagggccg ccagatctgc gccgagacga tccggatccg gctctgcggg
    16021 ccgcgcgcgg cggcgcccgc gagcgtggtc cagccgctct tcctgccgga tctgcccgtg
    16081 ttcctgcgct ggcgcggtgt gccttcgttc ggcagcgatt cgttcgagag cctcgtgggc
    16141 gtcgtcgacc gcctgatcgt cgacagcacc gagtggaacg agctacccgt ggcctacgcg
    16201 caactcgcgg gtgtcttcga tcgtgtcgtg gtctccgaca tcgcctgggc gcggacgagc
    16261 cggtggcgca ggcagctcgc atccctctgg ccggggatcg ggaacgtcga gcggatccgc
    16321 gtgaccggca ccgctgcgca ggcggagctc ctcgccggct ggttgcggtc acgcctgtcc
    16381 cgcccggttc aactcgagca cgagccggcc gaccacctcg aggccgtcga gatcgacggg
    16441 aaaccggcgc cgttccctcc gggtgacgca ccgccgccat cagacctgct ctccgaagag
    16501 ctcgaccggt tcgagcgcga ccgcatctac gaagaagccg tacgctccgc ggcgggatga
    16561 acgacttcac cgtgcacgtc aagccgctca tcgcggaggt gcccggctgg ctgacggacg
    16621 aagaagggga ggcgctctac gatctcgcgc gcgcgtgtac cggaaagggc gtgatcgtcg
    16681 agatcggctc gtggaagggc aagtcgacga tctgtctcgg gcgcggctcg ctggccggag
    16741 tgtccgtgcc catctatgcg atcgacccac atgccgacta tcgcttcgga gacttcaagg
    16801 cgaacgtcga ccgcgcggga atcacggagc tcgtcaggcc ggtcacgtcg ctctcacaac
    16861 ccgcggcgga cgagttccac gaaccaatcg agctgctgtt cgtcgacggc tcgcacgaat
    16921 atgacctcgt gctcgaggat ttcgagaagt gggtaccgaa ggtggtcgag ggaggctggg
    16981 tcgcctttca cgacacgacg tggacgtccg ggccccgcaa ggtcgtgggc caggcgatct
    17041 accgttcgaa ccgcttcaag gatgcgaggt tcgtggtcgg ctccaccacc gtcgcacgca
    17101 aggtcgatca gaactcgttc gccgaccgtg cgcgcaaccg ctacgtcctt ggcgtcaaga
    17161 cagcgttctg gctcggctcg accgttgtga agaagcaacg caggctcttg ccgaagcccg
    17221 tcgagcgcat cgggcgccgg ttcctcaagg cgatccagta gccgttccga gcttgtggct
    17281 cagagccaca agccaacagt gcgcgtcgcc ggcaacgctt tccagcgcct ggcctctcga
    17341 cttctgactt agtggctcag agccactaag tcagttcgtg ttacttgccg cctccgatga
    17401 cgcggaactt cggtttcgcg acgacgatct tggccgggtc ggaccaggac atctgctggg
    17461 cacccgtggg gagtgtgtag ttgaacccgc gcacgcggta gtaccaggtg cccggcttca
    17521 acgggaggac gtaagaggtg ccgagcgtga gcgtgccgag tgcgctctgg ttctggggat
    17581 tcggcagcgg cttgaaggga tagcgcttct tgctccactg gacctcgtac acggtggcgc
    17641 cgagtgccgg ggtccaggca acgagcgggg agccgtagaa cgcctggctc gaagttcccg
    17701 ccgtcaggcg acccttgggg gagaggccgg aggcgaagag ctcgcccgcc gcggtgagcg
    17761 acggctcgct ctgcttgcca aagcgtgcaa cccggttgta cggcggtgcg caaacgtcct
    17821 ggggcagctc cagatctcgg tactgcagac tgccaccgga gcgaacgagt ggctcgccga
    17881 cgccgtgccc gaacttcagg ggcgccgcca gtgtcagcgt ggtgccgctc acagcgatga
    17941 ccgtgaccgg ttcctggttc gccttgttcc cgagcagcac cgtgtcgccg gtcgagaagc
    18001 cgagcgtgct ggcgacggtc acgctggaca cgccgagcgc ggcggtcgcc gcggtactgg
    18061 tcgagagtgc gccgggggag agggcggcaa cggggatgac ggtccagtag tagccgctgc
    18121 tcggccaatc ggtgtcccac agatcgaccg gcgcaccgag cactcccgca ccgacttcaa
    18181 tctgctcagg cccggcaggc ggagaggggc ccgagccgcc ggggtttgta tcggaatcgc
    18241 tcggcacggc cgtcgtgggc ttcgcgggtg ccgccgcctc ggtcgtcttg acctgcaggc
    18301 cgtcgacggt gtagccggtc gcttcggagc cgtccgggag atagctcgcg cgggcagctg
    18361 ccatcgcggc ggtgacggaa ggaagcgcga gtgggccgaa gggacgcggc gcatatgcgg
    18421 gtcccccgat cactgcgctg gtgtacacgc gattgaggca ctgcctatcc gtgaagacgt
    18481 agatgcggaa gagctcggcg ctcgtgccat cgagagtctg atcgcccgtg aaggtgaacg
    18541 ccggcatcag ccgatgcgaa tcggaccctg catcgccggt tgaaacgatg tccgacactg
    18601 tgctgccgag ggtgatcggc ccgccctgat atgccgggtt ggtggagttg tagacgccac
    18661 tccagggccc gtagccaacc gcgggcagtc cgttctgcct cgtcttcttc tcgttgtcag
    18721 tccgatcggt gcggagcgcg cgaatcctcc agcgaaccga gctcgtccaa ttcgaggtgc
    18781 ggtggaaggt gtacagctcg cgctcatcga gcacgttggt gaagaccgtc tctatcttcg
    18841 gcatgtccac gaaccagacc tcgtagccgt ccgctccctc gatcggcgtc caccgaagca
    18901 ggcccgggta gctgggcagc ggcttgggcg cggcgcccgg ttccatgtcg aagccgaacg
    18961 aggcgctcca cggggtcgtg gaggaagccg tgattccacg gacccgtgcg aacagcgcgt
    19021 gcgggttgcc cgtgatccac gggagcatca ccgtaggtgc ggctacggga gtggtcagcc
    19081 cggtggtcga atacacgatt ccgctctcgc ggaaactgga gctcgttgcg agttggaact
    19141 ggtagcgaag cgcgcccggc gccgggttcc aggcgaagga gggcgtgcgc gggaaggagt
    19201 ttcggaccgg ctcgtcggcg cgcagcagga agccgtgcag cgcagtcggc ggggcaccgc
    19261 gaacagcagt cgccgcctcg cccgcgggcg cgagcaagag caggccgaga cagacggcgg
    19321 caagaagagg ggtccgcatg gttctctctg gttgtcggca ggcccggccg cacggctgtc
    19381 cctcgatcga gggacgccag ccggcgactc tggagttact tgcggcgctt cggtttcgtc
    19441 gtctggacag cgacgcgacg cgtgatggtc tcacccctgg cgccgccggg cgagtagctc
    19501 gtcaacgaca ggatgttctc cgagctggtc ttccgcaagc gcttgaggag ctgcgtcgga
    19561 agcacgaagc ggatgtcgtt gttgcccgcg tgcagggtgg cggaaccgtt ccagctcgag
    19621 ccgaggacgg cgcggagcgc tcccgttccg ctcgagaaga cgacgaagcg caagacgtgc
    19681 gtcttcgccg agacgcggag gagctcgggc gcgtagacgc tcaggtccgg cgccgggtcg
    19741 gtcgtcggca cctggatgac gggcccgatc accttgacgc ccggaggcgg cgacgcggcg
    19801 ctcgctggtt tcgacttgtc gacggtgaga aagccagtga cgacgaactc acggccgtcc
    19861 ttgggtgggc cgacctgatc ggcgtcgttc caatcgttcg ggagggacag ataggcgtgc
    19921 gggctcccgc tgcacgtgtc ccagggaacg tgatcgatcg acgctgtcga ccccgagacg
    19981 gtgagcgcct gcggcacaga cccagcgacg ttcgcgtagt agtagggctt cgagacgacg
    20041 ttggccggaa tgcggacctc gagcttcaac gaagcgacgt agcacggtcc cgtatcggtg
    20101 tcgtcaccgt gggccagctc gaggtagcgg accgcgaggt ggttcacggc gatgggggtg
    20161 accgagaaca atccgctcgc gttgccgagc gagagcgtcg ccagcgactg tggcaagacg
    20221 ccggccaagg ccggggacga aaagtctccg ttgagcctcg ccgtcgtgta gtcgttgaag
    20281 aagtcctgca gctttacggg cgcgggcaag accgatgcca gcgcctgggt tccggtcatc
    20341 ggcgctgccg agaggatgtc cttgatcttc gcgtcgccat agtgttcggt caggtactcg
    20401 aaaagcatcc agcccgggta gccgttcttg tcgaactcgg cgtccccgca ctcggcgccg
    20461 acgcagtcga gcgttcggtc cggattgact tcgagcccgc cggcagcgcc gtcggccttg
    20521 acggccgccc attcggctgt gccctccgcg acccaggggt ccgcgctggt tgtgatcttg
    20581 ccttgaaaga gatggaagac ttcgtgtgcg atcacgtggt acgtcaggcc cttcaccgtg
    20641 tcgagatgaa tctcgccggc gccgagcggc aggacggggt tgatcagcgc gtcccagcgt
    20701 cccagcgtcc cgtcgacctc gcggggaatg ctcaggtcga tcgcgccgtg ggcgatacag
    20761 ccgttgggcg ggagactgaa ctggtcggct gagatgtcca ggtagccgtc accatcgctg
    20821 atgtcgagga tcggggtcgc gtatccccag ctcgcgtaga gcgcataagc ccgctcggcc
    20881 atccccagaa cgtcccctgc ctgctcctgc gtgatgaaca tggtgggaca gacggtgctg
    20941 tggtcctcgc ggttgt
//
LOCUS       DASVEU010000107        12622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1236181,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000107 DASVEU010000000
VERSION     DASVEU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236181"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF225_04100"
     CDS             complement(<1..443)
                     /locus_tag="VF225_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144469.1"
                     /translation="MSGRTALVKGTTDGIAVSRRHGAAFWAVAFAFLVVMAFATLPSP
                     LYGLYRTRDHLSTLTVTVVYAVFACGAIAALLCEHVVVARIGRRGAMRGGVATVMVAA
                     AVIAVWKALPGLVVGRVLTGVSVGLAAGTATTYLIELRLRDDPNAS"
     gene            650..1165
                     /locus_tag="VF225_04105"
     CDS             650..1165
                     /locus_tag="VF225_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144470.1"
                     /translation="MLGRTSLLILGALSLAVVALAGCGGSASHALPTTSDGRPAIVGV
                     ASTGLGDVLVDRQGRTLYLFARDSGTVSACTGACAVNWPPLLAQGAPLVGTGATPSGV
                     GRTTRPDGLSQLTYNGHPLYTFVNDKKPGDTNGEGISAFGGSWFAVSPAGAKVAPRSQ
                     PQGGGGYGGGY"
     gene            1086..1511
                     /locus_tag="VF225_04110"
     CDS             1086..1511
                     /locus_tag="VF225_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144471.1"
                     /translation="MRSPLPAPRSPLAHSRKAGAVTAAATKPIRCLIVDDHQPFLAAA
                     RLLLEREGVVVVGVAATSAEALQLEEELRPDVVLVDIRLGGESGFDLARRLRSPVILI
                     STYARREYAEEIAASPATGFISKTELSASAVMQLVGATD"
     gene            complement(1517..2173)
                     /locus_tag="VF225_04115"
     CDS             complement(1517..2173)
                     /locus_tag="VF225_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144472.1"
                     /translation="MSINARVVLADDEVLMREGLAGLLERSGFEVVGQSGNASELLDL
                     VRELRPDLVIVDIRMPPTHTTEGLEAAKAIREEFPDIAVVALSAHVQVERATALLASG
                     ERSGYLLKSRVTDVDDFVDTLERILKGASVVDPALVQELIAARGVRDPLDGLSPRERE
                     VLALMAEGRSNAGIAHTLWITESTVEKHVRAILRKLRLPAGEDDQRRVLAVVTYLNAR
                     "
     gene            2202..4373
                     /locus_tag="VF225_04120"
     CDS             2202..4373
                     /locus_tag="VF225_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7144473.1"
                     /translation="MSTSAPPADPRNEQRLVEEQAALRRVATLVASGAPSAEVFSAVA
                     QEVAQVMHLPMVGVYRYDSDGLMTVIATWSDRPHVLHPGTRWPLDGQSMLAQVQRTGR
                     PARVEDYTDLPGALAAGARESGLNASAGAPIIVNGSVWGAVGAASPDAPLPEEVEDRL
                     AEFTELVATAIANSQAQDERTRLAEEQAALRRVATLVAEGATPQRVFDAVRREVARKF
                     NTPVSMLMRYDDANGTATLVTTAEGFLGPVGRSWPVEGDSSATAQVCRTGLPARADYT
                     RPTQGPIAAAARAAGGRSAVGVPVVVQGALWGVVTVGTRGAEPLPADVEGQLAKFTEL
                     LATAIARSEAHEQLALLADEQAALRRVATLVAEGASPHRVFDAVRGEVARMFNAPLSG
                     LLRYDGNGTVTQLAASDDYLGPIERSWTVEGDSAVAQVCRTGLPARADYTRPTQGSIA
                     ATARREGALSAVGVPVVVDGALWGVMAVGTREAEPLPADLEDRLAKFTQLLATAIANA
                     EGRAELDASRARIVATADATRRRIERDLHDGAQQQLVWLALALRAAQATVPEELEEHR
                     SELGRVVDGITAALDDLREVALGIHPAGLSEDGLTPALNRLVARSPLRVNLDCRADDR
                     FPEPVEVTAYYVVSESLTNAAKYADTPAVDVAVAADDGALRVEVRDEGRGGADPSKGS
                     GLLGLRDRVEAIGGTMRLTSPAGAGTSLRVELPLADQAARR"
     gene            complement(4663..5265)
                     /locus_tag="VF225_04125"
     CDS             complement(4663..5265)
                     /locus_tag="VF225_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7144474.1"
                     /translation="MNDVLVLPTGPLADASLALVRSTESRPIADHSIRSFIFARLLAE
                     HEGALNDAAYDEDLLFAAAVMHDLGLGEHAQGQARFEVEGADLAAEVLRAHGVPEADV
                     DRVWEAIALHASQGLAERRGLLTYLTHKGVFIDAGRIADALADELRREVQNAYPKPTD
                     GPYIADAIIAHGQRSPAAAPPGSLAGYLILQQRQLEHRHR"
     gene            5490..6410
                     /locus_tag="VF225_04130"
     CDS             5490..6410
                     /locus_tag="VF225_04130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7144475.1"
                     /translation="MRVALLQELGGIDSVELQERPDPTPAAGQAVVKVGGAGVGPWDV
                     AAISGAFGRPALPYIPGFEVAGVVEAVGDGVDVRPGERVWASLGIPGGGFAEYALANV
                     DSLGRMPDRLSFEEAAGLVVGGGTAWDGLLVRGQLQAGETVLITAAAGGVGSAAVQVA
                     AAVGARPLGVASPRNRDYLRGLGASEVFDYHAGDWVQQVLAVVPGGVDVLFDAAGGQT
                     RDQAVGAVRDGGRAIFLVGAPDQLGRGVAGEALDAEINRQRLDAIGRLVDEGKLRPQI
                     EAVLPFEQVREALERVGGRHTRGKVVLQIG"
     gene            6521..6667
                     /locus_tag="VF225_04135"
     CDS             6521..6667
                     /locus_tag="VF225_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144476.1"
                     /translation="MSYPLIVLLAIAMGIQKAAVRQLAVPVSLLTYRASRTSPEWARS
                     ATQS"
     gene            complement(6742..6972)
                     /locus_tag="VF225_04140"
     CDS             complement(6742..6972)
                     /locus_tag="VF225_04140"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEX7144477.1"
                     /translation="MDAWLRSARDAVAAASGVPVGELELTPGDARILLDLARAAAHDS
                     GQRTNAPLLCYLVGLAAAKSAAGLDEIALAVG"
     gene            6992..7774
                     /gene="fdhD"
                     /locus_tag="VF225_04145"
     CDS             6992..7774
                     /gene="fdhD"
                     /locus_tag="VF225_04145"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7144478.1"
                     /translation="MSEAPPYSASPVEVVRLPGGRTESDAVAVEEPLEIRINGEPVAV
                     TMRTPGHDEELALGFCLSEGLAPRDAALPRDLSANTVEVTASDFVPTEVKRNFFTSSS
                     CGVCGKGALEAVAVEAARVEIEMRVPASVISELPGRLREAQAAFSVTGGLHATGLFAA
                     SGELLCVREDVGRHNAMDKVVGWAFREGHLPLGNALLCVSGRLSFELVQKAAVAGCPL
                     LVAVGAPSSLAVELAADRGVTLCGFVRDGKINVYSEPWRVSD"
     gene            7756..8292
                     /locus_tag="VF225_04150"
     CDS             7756..8292
                     /locus_tag="VF225_04150"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX7144479.1"
                     /translation="MARERLTGVLLVGGASRRFGSPKALARFEGETLAERAWRVLGDV
                     CDERLAVGKTVDELPLPFPVLDDASDVRAPLVGVVAGLREAKNELCVILPVDCPLISA
                     GSLLQLADTGAAAAVPPSGPLPGAYRRTLLPLLERKLSGGDYSLRDVEATVVGLDPRE
                     LENVNTRLELAALQAARL"
     gene            8423..9757
                     /locus_tag="VF225_04155"
     CDS             8423..9757
                     /locus_tag="VF225_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7144480.1"
                     /translation="MNHLAAIVAALGPGEQKYLSQARQMQALSFAVHIPLVAFGIAFP
                     AMILFVESRYLRTGDELYRTLARRWSRILAALFAAGVVTGTILSFELGLLWPNFTGTF
                     GSVFGLGFAIEGFSFFMEAIFIGIYIYGWNRLSPRMHFASGIPIAIAGFTGSLTVISV
                     NGWMNHPIGFRYSHGRVTDVHPLTALFGNSYFWHELTHMYLAGYMVAGFVLASAYASG
                     RLGGRWGRYERTALAVPLAVATLASVAQIFIGDWNGREVATNQPTKLAAFEGLGKTTK
                     GAPVHILGWYTDGHVEYGIPLPRLLSLLAFHSTDATVKGLDAVPSADRPPVNVVRYAF
                     QTMVGIGTGLALLSVVYLATLARRKRLPESTWFYRALVVAGPLSVVALIAGWVTTEVG
                     RQPWVVYEVMRTSEAVTGADVIPVGYATLALVYLALAAGVAWVLLRLSRRST"
     gene            9757..10983
                     /locus_tag="VF225_04160"
     CDS             9757..10983
                     /locus_tag="VF225_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7144481.1"
                     /translation="MHLYELPLLFALVGLALYTVLGGADFGAGFWQLVAGRGERADEI
                     RDEAHHAMAPVWEANHVWLIFVLTVVWTAYPAGFGSIASTLSIPLFIAAIGIVMRGGA
                     YALRAGTRTAGEQRRVDTVFAVSSVLTPFALGTVIGAIASERVHVGNPGGSLVTSWLN
                     PTSIAIGVLAVASGAYTSAVFLCGDAIRRGEHALAERFRNRALGAGLAAGGAAIAGLI
                     VLHFDAERIFRRLTQGVGLPALIVSVLAGLATLALVWRRRYEPARYTAAIAVAATIAG
                     WALAQNPVFLHGLTIREAAAGHDTLVAVVVAILVGALLLFPSLALLFRLTLGGELRDS
                     GQPSGGAPVRSRRRALVSIRLDARLSVAFLVAGIGFLTVANAQWAHAIGVVCFFGFIL
                     SGYRAALPADVVDAGE"
     gene            11118..>12622
                     /locus_tag="VF225_04165"
     CDS             11118..>12622
                     /locus_tag="VF225_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7144482.1"
                     /translation="MAPPPDERQDAEQRMLGAVAGVPALGRGPAPDDDYSRHEYHHPA
                     AGWGAARSVGKVLERAGEPLEGFRALVVMNQENGGFDCPGCAWPDDPNGLHLDICENG
                     VKHVTWDLAPPKADSEFFAAHTVSELAAWSDYDLEAVGRLAEPMSYDPASDKYAPISW
                     EDAFALVGNTLRGLKSPNQAAFYTSGRLSNEATFLYQLWVREFGTNNLPDCSNMCHEA
                     SGRALAAAIGSGKGTVDLHDWEAADAIWLMADNAATNAPRMLTWLAEADRRGAQLVHI
                     NPLIEAASRRTIVPHEFVDMATFHATEIGTMNVQVRIGGDLALLRGVAKAVFEEAEKD
                     PNVLDREFIEQYTHGIDEYRALVEATPWGDLVRDAGVDELEIRKLAGSYLASKRVIIA
                     WCLGLTQHEHGVDTVREIVNLLLLRGNIGREGAGPCPIRGHSNVQGNRTCGIDHRPGK
                     AFLDRLGDVCDFEPPREHGLGTVATIEGMKSGDVKVFVALGGNFALASPDLP"
BASE COUNT         1958 a         4264 c         4367 g         2033 t
ORIGIN      
        1 gacgcgttcg gatcgtctcg aagtcgtagc tcgatgaggt atgtggtggc ggttccggcg
       61 gcgagcccga cggacacccc tgtgagtacg cgccccacga ccaaacccgg tagggccttc
      121 cagacggcga tcacggctgc cgccaccatc accgtcgcca ccccgccgcg catggctcca
      181 cggcggccga tgcgcgcgac gacaacgtgc tcacagagca aggcggcgat cgcgccgcac
      241 gcgaataccg cgtagaccac cgtgacggtc agagtcgaaa ggtggtcacg cgttcggtac
      301 agcccataga gcgggctcgg caaggtcgca aatgccatga cgaccagaaa ggcgaacgcc
      361 acggcccaga acgcggcacc gtgccgtcgg ctgactgcga tcccgtcggt ggtgcccttg
      421 accagtgcgg tccgtccact catgttgcac tcctcttctg gattggcgtc cacgctcacg
      481 ctgctcgtcc caggacgacg aactcgccgt cgagtcgcca agacagcgtg gcggggaggc
      541 gcagcgcccc gcatcgagac gaaccgcaag tcgttactgc ggttatccgc taacggcgtg
      601 tccggctcgc agcgatgcgt tccgagcgtg cggtggcgac catgagcaga tgcttggccg
      661 cacgtcactc ctcatcctcg gcgcgttgtc gctggcagtc gttgcgctgg ccggctgcgg
      721 cggcagcgcg tcacacgcgc tcccgacaac gagcgacggt cggcccgcca ttgtcggagt
      781 agccagcacc gggctcggtg acgtcctcgt cgatcggcag ggacggacgc tgtacctctt
      841 cgcgcgagac tcgggcacgg tgagcgcatg cacaggtgcg tgtgcggtca actggccgcc
      901 gctgcttgcc cagggcgctc cgctggtcgg caccggcgcc acgccgtcgg gagtcggaag
      961 gacgactcgc cctgatgggc tcagccagct cacctacaac gggcacccgc tatacacatt
     1021 cgtgaacgac aagaagccgg gcgacacgaa cggcgagggc atcagcgcgt tcggcggctc
     1081 ctggtttgcg gtctcccctg ccggcgccaa ggtcgcccct cgctcacagc cgcaaggcgg
     1141 gggcggttac ggcggcggct actaagccca ttcgttgcct gatcgtcgat gaccatcaac
     1201 cgttcctcgc tgcagcgcgg ctgctgctcg agcgtgaagg agttgtggtt gtcggcgttg
     1261 cggcgacgag cgccgaggcg ctgcagctcg aggaggagct tcggccggat gtcgtgctgg
     1321 ttgacatccg cctcggcggc gaaagcggct tcgatctcgc acggcggctg cgcagccccg
     1381 tgatcctgat ctcgacctac gcgcgacgcg agtacgccga ggagatcgcg gcgagccctg
     1441 cgacggggtt catctcgaag actgagttgt cggcgtccgc ggtcatgcag ctcgtcggcg
     1501 cgacggattg agggcgctaa cgcgcgttga ggtacgtgac cacggcgagc acgcggcgtt
     1561 gatcgtcctc cccggcaggc aagcgcagct tcctgaggat ggcacggacg tgcttctcga
     1621 cggtcgattc ggtgatccac agcgtgtgcg cgatacccgc gttcgagcgg ccctcagcca
     1681 tcagggcgag cacctcccgt tcgcgcggcg acaggccgtc gagcgggtcg cggacgccgc
     1741 gcgcggcaat caactcctgg acgagcgcgg gatcgacgac cgaggcgccc ttaaggatgc
     1801 gctcgagcgt gtcgacgaaa tcgtcgacgt ctgtgacacg actcttcagc aggtagccgc
     1861 tccgctcgcc actcgcgagc aacgcggtcg cgcgctccac ctgcacatgc gcggagagag
     1921 ccacgaccgc gatatccgga aactcctcac ggatcgcctt cgcggcctcg aggccctcgg
     1981 tcgtgtgcgt aggcggcata cggatgtcga cgatcaccag gtcgggccga agctcgcgga
     2041 ccaagtcgag cagctcggac gcgttcccgg actgacccac cacctcgaaa ccggaacgct
     2101 cgagcaggcc cgcgagaccc tcgcgcatca ggacctcgtc gtccgccagg acgactcgcg
     2161 cattgatgct catcgcaacg attatgggct accgtaacgt gatgagcaca tccgccccac
     2221 cggcggatcc gcgcaacgag cagcgactcg tggaggagca ggccgcgtta cgccgcgtcg
     2281 cgacgctcgt ggcgtcgggc gcgccgtccg cggaggtgtt ctcggccgtt gcgcaggagg
     2341 tcgctcaggt gatgcacctg ccgatggtcg gggtctatcg ctacgacagc gacggcctga
     2401 tgacggtcat cgcgacgtgg agcgaccgtc cgcacgtgct ccacccggga acccgctggc
     2461 cgctcgacgg ccagtcgatg ctcgcgcagg tacaacggac cggccgcccg gctcgggtcg
     2521 aggactacac agaccttccc ggcgcgctcg ccgccggagc tcgtgaatcc gggctcaacg
     2581 cgagtgcggg cgccccgatc atcgtcaacg gctctgtctg gggagccgtc ggggcggcgt
     2641 cgccggacgc accgctcccc gaggaggtcg aggatcggct cgcggagttc accgaactgg
     2701 tggcaaccgc gatcgcaaac agccaggccc aggatgagcg cacacggctc gcggaggagc
     2761 aggcggcgct ccgacgagta gccacgctcg tcgccgaggg ggcgacgccg cagcgtgtgt
     2821 tcgacgccgt ccgacgcgaa gtggcacgga agttcaacac cccagtcagc atgctgatgc
     2881 gctacgacga cgccaacggc accgcgaccc tggttacaac cgccgaaggc ttcctcggcc
     2941 ccgtcgggag aagctggccc gtcgagggtg acagcagcgc caccgcgcag gtctgccgaa
     3001 ccggcctccc agcgcgcgcc gactacaccc ggcccacgca ggggccgatc gcggccgctg
     3061 ctcgggccgc aggcgggcgg tccgcggttg gcgtgcccgt cgtcgtccaa ggcgctctct
     3121 ggggcgtcgt gaccgtcggc acgcgcggag ccgagcccct accggccgac gtcgaaggcc
     3181 agctagcgaa attcacggag cttctcgcga cagcgatagc aaggagcgag gcacacgagc
     3241 agctcgcgct gctcgccgac gaacaggcgg cgctgcgacg cgtggccacg ctcgtggcgg
     3301 agggggcgag cccgcaccgt gtgttcgacg ccgtccgagg cgaagtggca cggatgttca
     3361 acgctcccct gagcgggcta ttgcgctacg acggcaacgg caccgtgacc cagctcgcag
     3421 cttctgacga ctacctcggc cccatcgaga gaagctggac ggtcgagggt gacagcgcgg
     3481 tcgcgcaggt ctgccgaacc ggcctccctg cacgcgccga ctacacccgg cctacgcagg
     3541 gttcgatcgc agccacggcg cgaagggaag gcgcgctgtc cgcggtcggc gtgccggtcg
     3601 tcgtggacgg agctctctgg ggcgtcatgg ccgtaggcac acgcgaagcc gaacccctgc
     3661 cggctgacct ggaagaccgg ctggcgaagt tcacccagct cctcgctacg gcaatcgcca
     3721 acgccgaggg ccgcgccgag ctggacgcct cacgcgcacg aatcgtggcc accgccgacg
     3781 cgacccggcg ccggatcgaa cgcgacctgc acgacggcgc acagcaacaa ctcgtctggc
     3841 tcgcgctcgc cttgcgcgca gcacaggcga cggtgccgga ggagctcgaa gaacaccgaa
     3901 gcgagttggg ccgcgtcgtc gacggcatca cggccgcgct cgacgacctc cgcgaggtcg
     3961 ccctcgggat ccatcccgcc gggctctcgg aggacggcct gacgccggcg ctcaaccggc
     4021 tcgttgcccg ctcgccgctc cgcgtcaacc tcgactgtcg tgccgatgat cgcttcccgg
     4081 agccggtgga ggtgacggcc tactacgtcg tctcggagtc gttgacgaac gcagcgaagt
     4141 acgcggatac cccggctgtg gacgtcgcgg tagccgccga cgacggtgcg cttcgtgtgg
     4201 aggttcgcga cgaagggcgt ggcggcgcgg acccgagcaa gggttcaggg ctgctcggcc
     4261 tgagagaccg cgtcgaggcg atcggaggga cgatgcgtct aacgagccca gcgggcgcag
     4321 gcacctcgtt gcgcgtcgag ttgccgctcg ccgatcaggc cgctcgtcgc tagctgccgt
     4381 cgtcgtaatc gtcgccctcg tgccacccgg gacggctcgc gctcagcgaa cgatctcgag
     4441 ggcgattggg caacaatcag gcaacatcag tccgtgccaa caagggtttc tctgtccctg
     4501 tgcgtcccta atgtattccg acatctccag ggattcttcg gtctgggagg ggcacgtagg
     4561 gacagcctga gacatcccta acttgaatgg taaggagggg gtctccggtt cgagtccgga
     4621 agagggcttc ccgtcgaagg aggggtcagc agtccgagcc gctcaacggt gacggtgttc
     4681 gagctgacgt tgctgaagga tcaggtaacc ggcgagcgaa ccgggaggag ccgctgccgg
     4741 ggaccgttgg ccgtgcgcga tgatcgcgtc cgcgatgtac gggccgtccg tgggcttggg
     4801 gtaagcgttc tgcacttccc tgcgcagttc gtcagccagg gcatctgcga ttcggcctgc
     4861 gtcgatgaag acacccttgt gcgtcaggta ggtcagtaga ccgcggcgct cggcgagacc
     4921 ctgggaagcg tgcagcgcga tcgcctccca aacccggtcg acgtcggctt ctgggacgcc
     4981 gtgggccctc agcacctcgg cggccaggtc ggcgccctcg acttcgaagc gagcctggcc
     5041 ttgggcatgc tccccgagcc ccaggtcgtg catgacggcg gcggcgaaga gcaagtcctc
     5101 gtcataagcc gcatcgttca aggcgccctc atgttcggca agcaagcgcg cgaagatgaa
     5161 gcttcggatg ctgtggtcag cgatcggccg actctccgtc gagcggacga gcgcgaggct
     5221 cgcgtcagcc agcggccccg tcggaagcac aagaacgtcg ttcatggcag gagttgcacg
     5281 tgttccgacg agtctcatca ggcaaggggc caccgcgcat cacacgatcc tgcgaactga
     5341 aagtcacaac acgctcgaaa accgttatcc gtcgttaggc ggatcgaggg ttcaaatccc
     5401 tccccctccg cctcctcctc agccgaaggc ggcatggggc ccgtacgctg cctttgggca
     5461 ttgggagcac gagtcgagga gacacagata tgcgagtagc gctgctgcag gagctgggcg
     5521 ggatcgacag cgtggaactg caagaacggc ccgacccaac ccccgccgcg ggacaggcgg
     5581 tggtcaaggt gggaggcgcg ggcgtcgggc cgtgggacgt cgcggcgatc agcggcgcct
     5641 tcggacggcc tgcgctgccc tacatcccgg ggtttgaagt cgcaggcgtg gtggaggcgg
     5701 tcggcgacgg ggtcgatgtc cggccgggcg agcgggtgtg ggcgagtctg gggatccccg
     5761 gcggcgggtt tgccgagtac gcgctggcaa acgtggacag cctggggcgc atgcccgacc
     5821 ggctgagctt cgaggaggcg gccgggctgg tggtcggcgg agggaccgcc tgggacggac
     5881 tgctcgtccg ggggcagttg caggccggtg agaccgtgct catcaccgcc gcggccgggg
     5941 gcgtgggcag cgccgccgtg caggtggccg cggccgtggg cgcgcgcccg ctgggcgtgg
     6001 ccagcccgcg caatcgcgac tacctgcggg gcctgggtgc cagcgaagtg ttcgactacc
     6061 acgccggcga ctgggtccag caggtgctcg ccgtggttcc cggcggcgtc gacgtcctct
     6121 tcgacgctgc tggcggccag acccgcgatc aagccgtggg cgcggtgcgc gacggtggcc
     6181 gggcgatctt cctggtcggt gcgcccgacc agctgggacg gggcgtcgcc ggcgaagcgc
     6241 tcgacgccga aatcaaccgg cagcggctgg acgcgatcgg tcggttggtc gacgagggga
     6301 agctgcgccc tcagatcgaa gcggtactgc cattcgaaca ggtgcgcgaa gcactggaac
     6361 gagtcggagg tcgacacacg cgcggcaagg tcgtactcca gattgggtag gtaggcggtc
     6421 tcgacgccgt caggcggcga ggctggtctg gatcgtcgct cctacttcgg tcaaaagttg
     6481 aagtgagact tcccggactt gctggtcgag attggagtcg ttgagttatc cgctgatcgt
     6541 gctcctggca atcgccatgg gcatccagaa ggcggccgtc cgacagcttg cggtccctgt
     6601 ctccctcctg acctaccgtg cgtcccgcac atcgcccgag tgggcgcgat ccgccacgca
     6661 gtcctgatcg ttcaaccaag tggggtcgac taccgcttcc cgtcgcgccg ccgacaggta
     6721 cgcgaagtcc gttgtggcga tctagccgac cgcgagcgcg atctcgtcta gccccgcagc
     6781 gctcttggca gccgcgagac cgacgaggta gcaaagcaac ggcgcatttg ttcgttgccc
     6841 gctgtcgtgc gcggcggctc gcgccaggtc gaggaggatc cgcgcatcgc cgggtgtgag
     6901 ctcgagctcg cccacgggaa cccccgaagc ggcagcaacg gcatcgcgcg cagagcgcaa
     6961 ccaggcatcc ataatctggg acgctatcgg gatgagcgag gcgccgccgt actccgcatc
     7021 accggtcgag gtcgttcgac ttccaggcgg tcgtacagaa agcgatgcgg tcgcggtgga
     7081 ggagccgctc gagatccgca tcaacggcga gccggttgcg gtgacgatgc ggacgccggg
     7141 gcacgacgag gagctcgcgc tcggcttctg cctctcggaa ggcctcgcgc cgcgcgacgc
     7201 cgcgctgccg cgtgacctct ccgcgaatac cgtggaggtc acggcgagcg acttcgtacc
     7261 cacagaagtg aagcgcaact tcttcacgag ctcgtcgtgc ggcgtttgcg gcaagggcgc
     7321 gctcgaagcc gtcgcggtcg aagcggcgcg agtggagatc gagatgcgcg tgccggcgag
     7381 cgtcatctcc gagctgccag gacgcctgcg ggaagcgcag gctgcgttca gcgtcaccgg
     7441 cgggctccac gcgaccggtc tcttcgcggc ctcgggcgag ctgctgtgcg ttcgagaaga
     7501 tgtcggtcgg cacaacgcga tggacaaggt cgtgggctgg gcgtttcgcg agggtcacct
     7561 tccgcttggg aatgcgctcc tctgcgtcag cggccgcttg tcgttcgagc tcgtgcagaa
     7621 ggcggctgtg gccggttgcc cgctgctcgt cgcggtcggc gccccgtcgt cgctcgccgt
     7681 cgagctcgcg gcggatcgcg gggtcacgct ctgcggcttc gttcgcgacg ggaagatcaa
     7741 cgtctactcg gaaccatggc gcgtgagcga ctaacaggcg ttctgcttgt cggcggcgcg
     7801 agccggcgct tcggctcgcc gaaggcattg gcccggttcg agggcgagac actggccgag
     7861 cgcgcgtggc gcgtgctcgg ggacgtgtgt gacgaacggc tcgccgtggg gaagacggtg
     7921 gacgagttgc cgctgccgtt tccggtgctc gacgacgcgt cggatgttcg tgcgccgctc
     7981 gtcggggtcg tcgcgggctt gcgggaggcg aaaaacgagc tctgcgtcat tcttcccgtc
     8041 gattgcccgc tgatctccgc cgggagtctg ttgcagctcg ccgacacggg ggccgccgcg
     8101 gcggtgccgc cctccggccc gctcccaggc gcctatcgcc ggacgctcct gcccctgctc
     8161 gagcggaagc tgtcgggcgg cgactactcg ctgcgcgacg tcgaggccac cgtcgtcggg
     8221 ctcgatccac gcgagctcga gaacgtcaac acgcgcctgg agctcgcggc gcttcaagcc
     8281 gcgcggctct agccgcgcgg ctctgacagg ctagcctcgc cgtagccgca cagcggccgg
     8341 tgcggtcgcc caacctccag gcggcggcgc tcggctcgtt tgttgtcggc tacctggttc
     8401 tcgctctatg gtccccgagc agatgaatca cctcgcggcc atcgtcgccg cgctcgggcc
     8461 cggcgagcag aaatacctgt cgcaggcccg gcagatgcag gcgctctctt tcgccgtgca
     8521 catccctctc gtcgccttcg ggatcgcctt cccggcgatg atcctcttcg tcgagtcgcg
     8581 gtacctgcgg accggcgacg agctctaccg cacactggcg cgacgctggt cgcgaatcct
     8641 cgcagcactg ttcgcggccg gcgtcgtcac cggcaccatc ctcagcttcg aactcggact
     8701 gctctggccg aacttcaccg gcactttcgg cagcgtgttc gggctcggct tcgcgatcga
     8761 aggcttctcg ttcttcatgg aggcgatctt catcggcatc tacatctacg gctggaaccg
     8821 cctctcgccg cgcatgcatt tcgcgagcgg aatcccgatc gcgatcgccg gcttcaccgg
     8881 ctcgctgacg gtcatctccg tcaacgggtg gatgaaccac ccgatcggct tccgctatag
     8941 ccacggccgc gtcaccgacg tacatccgtt gacagcgctg ttcggcaaca gctacttctg
     9001 gcacgagctc acgcacatgt acctggccgg ctacatggtt gccggcttcg tcctggcgag
     9061 cgcctacgcc tctggtcggc tcggaggccg atggggccgg tacgagcgga cggcgctcgc
     9121 ggtcccactc gcggtcgcga cacttgcttc ggtcgcgcag atcttcatcg gcgactggaa
     9181 cgggcgcgag gtcgcgacca accagccgac caagctcgct gccttcgagg gcttggggaa
     9241 gaccaccaag ggtgcgcctg tccacatcct cggctggtac acggacggcc acgtcgagta
     9301 cgggatcccg ctcccccgtc tgctctcact actcgccttc catagcaccg acgccacagt
     9361 gaaagggctc gatgccgtcc cgtccgcaga ccgcccgccg gtgaacgtcg tgcgctacgc
     9421 cttccagacg atggtcggga tcggcaccgg gctcgcgctg ctttcggtcg tttatttggc
     9481 caccctcgcc cggcggaagc ggttgccgga gtcgacctgg ttctaccggg cactggtggt
     9541 ggcgggacct ctctcggtgg tggcgttgat cgcaggctgg gtgacgaccg aagtcggccg
     9601 ccagccgtgg gtcgtctacg aggtcatgcg cacctcggag gcggtgaccg gggcggacgt
     9661 gattcccgtt ggctacgcca ccctcgcgct cgtctacctc gccctcgccg ccggagtcgc
     9721 ctgggtcctg cttcgcctct cgaggaggtc gacctgatgc atctctacga gctgccactc
     9781 ttgttcgcgc tcgtggggct cgcgctctac acggtgctcg gcggtgctga cttcggcgcg
     9841 ggcttctggc agctcgtggc cggcaggggc gagcgggccg acgagatccg cgacgaggcc
     9901 catcacgcga tggcgccggt ctgggaagcc aaccacgtct ggctgatctt cgtcctcacc
     9961 gtcgtctgga ccgcctaccc ggctggcttc ggctcgatcg cctcgacgct ctcgatcccg
    10021 ctcttcatcg ccgccatcgg catcgtcatg cgcggcggcg cctatgcgct gcgtgcgggg
    10081 acgaggactg ccggtgagca acgccgggtc gacaccgtct tcgccgtctc gtcggtcttg
    10141 acgccgttcg cgctcggcac cgtcatcggc gcaatcgcct ctgagcgcgt gcatgtcggc
    10201 aacccgggcg gaagcctcgt cacgagctgg ctcaacccga cctcgatcgc gatcggcgta
    10261 ctcgccgtcg cgagcggcgc ctacacctca gcggtctttc tctgcggcga tgccattcgt
    10321 cgcggtgagc acgcgctcgc cgagcgtttc cgtaacagag ccctcggtgc ggggctcgcc
    10381 gccggcggcg ctgcgatcgc agggctcatc gtcctgcact tcgacgcaga acgcatcttc
    10441 aggcgactca cccagggcgt ggggctgccg gcgctgatcg tctccgtgct cgccggtctg
    10501 gccacgctgg cactcgtctg gcgacggcgc tacgagcccg cgcggtacac ggctgcgatt
    10561 gctgtcgccg cgacgatcgc cggctgggcg cttgcacaga acccggtttt cctacacggg
    10621 ctcacgatcc gcgaggcagc ggccgggcac gacacgctgg tcgccgtggt ggttgcgatc
    10681 ctcgtcggcg ccctgttgct cttcccctcg cttgcgctcc tcttccggct gacgctcggc
    10741 ggcgagctca gagacagtgg gcaaccctcg ggcggggccc cggtcagatc tcgtcgccga
    10801 gcgctcgtga gtatccggtt ggacgcacgt ctctcggttg cgttcctcgt cgcaggcatc
    10861 ggctttctaa cggttgcgaa cgctcagtgg gctcacgcaa tcggcgttgt ctgtttcttc
    10921 gggttcatcc tctcgggcta tcgcgctgcc ctgccagcgg acgttgtcga cgccggcgag
    10981 tgacggcaac atgacgctcg agagcttgcg cgaatcccac tcgcgacgag caggaagacg
    11041 attttggctg gcgggggcac ggatgccgcc cgactgtgtc atccttgccc cgccggcgca
    11101 cctcggaagg agggacgatg gcaccgccac ccgatgagag gcaggacgcg gagcagcgga
    11161 tgctcggcgc ggtggcgggt gtccccgcgc ttgggcgcgg ccctgccccg gacgacgact
    11221 actctcgcca cgagtaccac catcccgccg cgggatgggg cgcggcgcgc agcgtcggca
    11281 aggtgctcga gcgtgccggg gagccgctgg agggctttcg agcgctggtc gtgatgaatc
    11341 aggagaacgg cgggttcgac tgtccgggct gcgcgtggcc ggacgacccg aacggactcc
    11401 acctcgacat ctgcgagaac ggcgtcaagc acgtcacctg ggatctcgcg cccccgaagg
    11461 ccgacagcga gttcttcgcc gcccacaccg tcagcgagct ggccgcgtgg agcgattacg
    11521 acctcgaggc ggtgggacgg cttgccgagc cgatgagcta cgacccggcg agcgacaagt
    11581 acgcgccgat ctcctgggag gacgccttcg cgctggtcgg caacacgctg cgcggattga
    11641 agagtccgaa ccaggcggcg ttctacacct cggggcggct cagcaacgag gcgacgttcc
    11701 tctaccagct ctgggtacgc gagttcggga cgaacaacct gccggactgc tcgaacatgt
    11761 gtcacgaggc aagcggccgg gcgctggcgg cggcgatcgg gagcggcaaa ggcacggtcg
    11821 atctgcacga ctgggaggcc gcggacgcga tctggctgat ggccgacaac gccgcgacga
    11881 acgcgccgcg aatgctcacg tggctggccg aggccgaccg ccgcggcgcc cagctcgtcc
    11941 acatcaaccc gctgatcgaa gccgcctcga ggcggacgat cgtcccccac gagttcgtcg
    12001 acatggcgac gttccacgcg accgagatcg gcacgatgaa cgtgcaggtt cggatcgggg
    12061 gcgatctggc actgctgcgc ggcgtcgcaa aggccgtctt cgaggaggcg gagaaagatc
    12121 cgaacgttct cgaccgggag ttcatcgagc agtacacgca cggcatcgac gaataccgcg
    12181 cactggtcga ggccaccccg tggggcgacc tcgtacgcga tgccggcgtg gacgagctcg
    12241 aaatccgcaa gctcgccggc tcgtacctgg cgtcgaagcg cgtgatcatc gcctggtgcc
    12301 tcggcctcac gcagcacgaa cacggcgtcg acaccgtgcg cgagatcgtg aacctgctgc
    12361 tcctgcgtgg caacatcggc cgggaaggcg cgggaccgtg tccgatccgg gggcacagca
    12421 acgtgcaggg caaccgcacc tgcgggatcg accaccgtcc gggcaaggcg tttctcgatc
    12481 ggctcggcga tgtctgcgac ttcgagccgc cacgcgaaca cgggttgggc actgtggcga
    12541 ccatcgaagg gatgaaaagc ggcgacgtga aggtgttcgt cgccctgggc ggcaacttcg
    12601 cactcgcgtc ccccgacctc cc
//
LOCUS       DASVEU010000108         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1431402,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000108 DASVEU010000000
VERSION     DASVEU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431402"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..711
                     /locus_tag="VF225_04170"
     CDS             391..711
                     /locus_tag="VF225_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX7144483.1"
                     /translation="MPKFLIEASYTLDGAKGVQSAGGTSRRDAVAQLAESVGGSLESF
                     HFAFGDSDAYVIVDLPDNESAAAVALTVNTSGAVTTKTVVLMTPEEVDAAAKRSVDYR
                     PPGA"
     gene            complement(763..837)
                     /locus_tag="VF225_04175"
     tRNA            complement(763..837)
                     /locus_tag="VF225_04175"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:His,seq:gtg)
     gene            complement(1087..1773)
                     /locus_tag="VF225_04180"
     CDS             complement(1087..1773)
                     /locus_tag="VF225_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144484.1"
                     /translation="MEPGRDETIGVLVVDDHEVVRRGLLAFLDSEPDIDVVGEAGGGA
                     QALDLLASMELEGRRPDVIVMDLQMAPIDGIESTRRVRALYNDIEVVALTSFAEEERV
                     HAALQAGASGYVLKDSDADDVAAAVRAAHRGELQIDPVVARRLMSSLGEARDDDPISE
                     LTSRELDVLRLVAAGKANKQIAAELAISERTARTHVSRILRKLHLSSRTQAALWAVRE
                     GLVEMEREPH"
     gene            complement(1775..>2010)
                     /locus_tag="VF225_04185"
     CDS             complement(1775..>2010)
                     /locus_tag="VF225_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7144485.1"
                     /translation="AGVSAAHVRVEVKQGQVVLEVRDNGRGFHPAAGHPGHFGLDSMR
                     SRAAEIGGRLTITSAPGSGTLVRVCVPAETDGA"
BASE COUNT          344 a          696 c          661 g          309 t
ORIGIN      
        1 cgctgcagac tgaaggggcg gcggaggcaa tgaagcacga cggcgtccgc cccgagacga
       61 tccttagtct cgtcgaggcg tagccggacg gttgcgcgtc actgaaggcg ggagcaccca
      121 caggctcccg ccttcaaagg cactcgacgg cgcgacgacg atcggcgtga cgccggcgta
      181 tgtcgcttgc gcgtgggtcc ctcgtacgcc taagaccaga actgctcgca ggactccacg
      241 atcgcgacgt ccgatgccgc attccagact gcaccagcac ctgaggttcg acaactagcg
      301 ctggcggcca actccgcgtg cctcggtgtg cttgctccgg actgcgcggg gtgagagggt
      361 gtgaccacgc cagcggcaca ggaggtttcc atgcccaagt tcttgatcga ggcctcatac
      421 acattggatg gcgccaaggg tgttcaaagt gcgggcggaa ccagccggcg cgacgcagtg
      481 gctcaactcg ctgagagcgt cggcgggagc ctcgagagct tccacttcgc ctttggcgac
      541 agtgatgcct acgtgatcgt cgacctcccc gacaacgaaa gcgcggcggc ggtcgcgctg
      601 accgtaaaca ccagtggagc ggtgacgaca aagacggtcg tgctgatgac gccagaggag
      661 gttgacgcgg ccgcgaagcg ctcggtcgac taccggccgc ccggagcctg acgagctcgc
      721 gcgccggagg gggtctccgc gcaaagcagc caagcgtgaa ggtgagcgac gggactcgaa
      781 cccgcgaccg cctggaccac aaccaggcga ccacctcacc attctcggag ctcgacgcac
      841 gcgcgcacgc gcgcgcggac acgcgcgacc agggaatcgc ggccgcgggc ggcgcactag
      901 ccgatatccg gctgattctc gggggaccgc cgggggaccg aaacctctga aaccagggct
      961 tccgctggct tgcgtagagc ggccgacgac ctccggcaca ccgcgatccc gctcgtagcg
     1021 cgggattgcg tctcctacag cggaggggga ggattcgaac aggatccgga ccttctcggc
     1081 ctccaactag tgcggctcgc gctccatttc cacaagccct tccctaaccg cccagagggc
     1141 agcctgggtg cgcgagctca gatggagctt gcggaggatc ctggatacgt gtgtcctggc
     1201 cgtgcgctcg ctgatggcga gctcggcggc gatctgcttg ttggccttgc ccgccgccac
     1261 gagacggagc acgtccagct ctcgggatgt gagctcggag atcgggtcgt cgtctcgcgc
     1321 ctcgccaagt gacgacatca gccttctcgc gacgacagga tcgatttgca gctcgcctcg
     1381 gtgcgcagcc cggacggcgg ctgccacgtc gtcggcatcg gagtccttga gcacgtagcc
     1441 cgatgccccg gcctggagcg cggcgtgcac gcgctcctcc tcggcgaacg acgtgagcgc
     1501 caccacttcg atgtcgttgt agagagcgcg aacccgacgg gtcgactcga ttccgtcgat
     1561 cggcgccatc tgcagatcca tgacgatcac gtcgggccgg cgcccctcca actccatcga
     1621 cgcgagcagg tcgagtgcct gggcgccccc tcctgcctca cccacgacgt cgatatcggg
     1681 ctcgctgtcc aggaacgcaa gcaacccgcg gcgcacgacc tcgtggtcgt cgacaaccag
     1741 gacaccgatg gtttcatccc gccccggctc catctcaggc gccatccgtt tccgcgggga
     1801 cgcagactcg tacgagtgtc ccagaccccg gcgcgctcgt gatcgtgagc cgcccgccga
     1861 tctcggccgc acggctgcgc atcgagtcga ggccgaagtg accgggatgt cccgcggccg
     1921 ggtggaagcc ccgcccgttg tcgcgaacct cgaggacgac ctggccttgc ttcacctcga
     1981 cccgaacgtg cgccgcgctc accccggcgt
//
LOCUS       DASVEU010000109         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1627152,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000109 DASVEU010000000
VERSION     DASVEU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627152"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..628
                     /locus_tag="VF225_04190"
     CDS             440..628
                     /locus_tag="VF225_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144486.1"
                     /translation="MTESESPSVASSTTVPVVAAPPVLPIPVVAVPVVAVPPVTVPLP
                     ELPVTVPELPATQPLPLP"
     gene            644..1201
                     /locus_tag="VF225_04195"
     CDS             644..1201
                     /locus_tag="VF225_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144487.1"
                     /translation="MIAAPDPPLTDGVVTLRQWEERDVATLVECIDGDEEMTRWMDAI
                     PQPYLDAEARTWIEQASSFWRTGTSAPFAVTDTETGDVLGGVGFAWIGDEGVGEVGYW
                     LRSEARGRGLTRRAVRLVSRWSFEELGCERLQLRADADNVASQRVAEKAGFTREGVLR
                     SVHFNPRQQRRVDFVMFSLLPSEVD"
     gene            complement(1198..2391)
                     /locus_tag="VF225_04200"
     CDS             complement(1198..2391)
                     /locus_tag="VF225_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7144488.1"
                     /translation="MASRVERLADLIVNFGANVQKGQILDVLSGLGKEELTRAITASA
                     YRRGAKFVDVHYWDPHLKRERVLYAPDDVLDFVPPWYGERALQLGHDRGATISLSGPI
                     EPHLFDDLDPERLGRDVFPRVKEWTTVINERTVNWCVAPGPSEKWAQLVFPDLEPQVA
                     LDKLWEQAVHVCRLDEDDPEAAWRDRNSILKGVAQRLSDRHFDSIHLEGPGTDLTVGL
                     LPTSRWLGGGDTTVDGIPHMANLPTEEVFSSPDPERTEGVVRATKPLTTQGRIIDGLT
                     VRFEGGRAVEIEAESAAETLRALVAQDEGGTRLGELALVDREGRIGPLDTVYYDTLLD
                     ENAASHIALGSAYDAAVGEDDVERLNRSSVHIDFMIGSPEVDVTGITRDGERVPVLRD
                     SSWQI"
     gene            2429..3265
                     /locus_tag="VF225_04205"
     CDS             2429..3265
                     /locus_tag="VF225_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7144489.1"
                     /translation="MKTTPAIERFLNSPALSEGTRRSYRHDVEEFGRWLERRNLGLDG
                     VDVRVLTEYVAELGRARPRKLAPATIARKVAAIRSFLRFTLGPTRVPDASLAPRRPKR
                     LPDTPKTADVEAALDALVGDGPLPLRNRALVELVYSAGLRSAEAVGLDLADVDFDREQ
                     LHVRGKGGKERIVPLGEEAGHLVAQYLHEARPQLAAGAENALFLSARGRRLDTSTLRR
                     VFAHPHSLRHAFATHLLEGGADLRTIQDLLGHSSLSTTQLYSHVDGRRLRRVYDRAHP
                     RS"
     gene            complement(3271..>3619)
                     /locus_tag="VF225_04210"
     CDS             complement(3271..>3619)
                     /locus_tag="VF225_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144490.1"
                     /translation="LTNSGTTATTCLLNDSPDWARPTSAGTYTGSLWVRADTAGKPLK
                     LRFREYSISSGALLGTATAQVTLTTSWQQVTVSYTITAPGSTLDFNAYLSSADAPPGT
                     CFYADDVAILANP"
BASE COUNT          534 a         1210 c         1252 g          623 t
ORIGIN      
        1 gtctctccgc cgaaatcgtc gctgctgtca gctaagcgct gtcccgaggt aacggttccc
       61 tcgcacgggg gttataggcc gtctggacta ccccgataag ggggaaatgg gactcgcaat
      121 gcgtaaaggc gagttgaggc ttggcctctc cgcgaccgtc gcgtctgcgg tcctgtacag
      181 cgcgatcgtg ctgttcggtg tcggctcagc ctccccctcg gctcccgatg ccggccgcga
      241 tccgaatgcg tcggccgtgc tgattcaagg gcatgccgac accgtctccg gctccgtgcc
      301 gaggctgccg caggcttcgc cggagccgag acggcaccgc gcgcacacaa gagcgcgtcg
      361 accccgcact gggatcgcgc cggcgcagac gccttccgcg ccctctccgg ttgccgcccc
      421 ggcgccggag tctccgcgcg tgactgaatc cgagtcgccg agcgtcgcaa gctccacgac
      481 ggtcccggtg gtggctgcgc cacccgtgct gcccataccg gtcgtcgccg taccggtcgt
      541 cgccgtgccg ccagtgaccg tccctcttcc cgaactaccg gtcaccgtgc ccgaactacc
      601 cgcgacccag ccgctgccgc ttccctgagc tagcctcggc ggcgtgatcg ctgcgcccga
      661 tccgcccctg accgatggag tcgtaaccct gcggcaatgg gaggagcgcg acgtggccac
      721 gctcgtcgag tgcatcgacg gcgacgagga gatgactcgc tggatggacg cgatcccgca
      781 gccatatctg gatgccgaag cacgtacgtg gatcgagcag gcctcctcct tctggcgcac
      841 ggggacctcc gcgcccttcg cggtcacgga caccgaaacg ggcgacgtgc tcggtggcgt
      901 cggtttcgcc tggatcggcg acgagggcgt cggcgaggtc ggctactggt tgcgctccga
      961 agcgcgaggc cgcgggctga cgcgtcgcgc ggttcgtctc gtctcgcgct ggtccttcga
     1021 ggagctcggc tgcgagcggc tgcagttgcg cgcggacgca gacaacgtcg cgtctcaacg
     1081 tgtcgccgag aaagcaggct tcacgcgcga gggcgtgctc aggtcagtgc acttcaaccc
     1141 gcggcaacag cgccgcgtgg atttcgtgat gttctcgctg ctgcccagcg aggttgatta
     1201 gatctgccag ctcgagtccc gcagcaccgg aacgcgttcg ccgtcgcgcg tgattccggt
     1261 cacgtcgacc tcgggtgagc cgatcatgaa gtcgatgtgg acgctggacc ggttcagccg
     1321 ctcgacgtcg tcctcgccga ccgccgcgtc gtaggccgag ccgagggcga tgtgactcgc
     1381 ggcgttctcg tcgaggagtg tgtcgtagta gacggtgtcg agaggaccga tgcgtccctc
     1441 ccggtcgacg agagcgagct cgccgagtcg cgtgcctcct tcgtcctggg caaccaacgc
     1501 acgcagggtt tcggcagcgg attccgcctc gatttccacc gcacggccac cttcgaaacg
     1561 caccgtcagc ccgtcgatga tccggccctg tgtcgtcagc ggcttcgtcg cacggacgac
     1621 cccttcggtc cgctccggat caggcgagct gaagacctcc tcggtcggaa ggttcgccat
     1681 gtggggaatc ccgtcgaccg tcgtgtctcc cccgccgagc cagcgggagg tcggcaggag
     1741 tccaaccgtc aggtcggttc ccggcccctc gaggtggatt gagtcgaaat gccggtcgct
     1801 cagccgctgc gcgaccccct tcaggatgct gtttcggtcg cgccaggcgg cctcgggatc
     1861 gtcttcgtcc agccggcaga cgtgaacggc ctgctcccac agcttgtcga gcgcgacctg
     1921 cggctcgaga tcgggaaaga cgagttgcgc ccatttctcg gaaggccccg gcgctacgca
     1981 ccaattgacc gtgcgctcgt tgatgactgt cgtccattcc ttgacccgtg ggaagacatc
     2041 ccggccgagc cgctccgggt ccagatcgtc gaagagatga ggctcgatcg gaccgctgag
     2101 agagatcgtc gcgccgcggt cgtgaccgag ctgcaaggcc cgttcgccgt accagggcgg
     2161 gacgaagtcc agcacgtcgt ctggcgcgta gagcacgcgc tcccgcttga gatggggatc
     2221 ccagtagtgg acatcgacga atttcgcgcc gcgcctgtaa gcgctggccg taatggcgcg
     2281 agtgagctct tctttcccga gcccagagag gacgtcgagg atctggccct tctgcacgtt
     2341 cgcgccgaag ttgacgatca ggtcggcgag gcgttcgact cgggaggcca tgggcaggcg
     2401 atgctacttg ggccgcgaat ggctttgcat gaagactaca ccggccatcg agcgtttcct
     2461 caactcgccg gcgctcagcg aaggaaccag gcgttcctat cggcacgacg tcgaggagtt
     2521 cggacgctgg ctggaacgac ggaacctcgg cctcgacggc gtggacgtga gggtgctcac
     2581 cgagtacgtg gccgagctcg ggcgcgcgcg tccgcggaag cttgctccgg ccacgatcgc
     2641 ccggaaggtc gcggcgatcc gcagcttcct ccggttcacg ctggggccta cgagggtgcc
     2701 ggatgcctcg ctggcgccgc ggcgtccgaa gcggctgccc gatacgccga agacggccga
     2761 cgtcgaagct gcgctcgacg cgctggtggg cgacggcccg ctgccgctgc gcaatcgcgc
     2821 cctcgtcgag ctcgtctatt cggcggggct gcgcagcgcg gaggcggtcg ggcttgatct
     2881 cgccgacgtg gacttcgacc gcgagcagtt gcacgtgcgc ggcaagggcg gcaaggagcg
     2941 gatcgtcccg ctcggggaag aggccggcca cctggtggcg cagtacctgc acgaagcgcg
     3001 gcctcagttg gcagcgggtg cggagaacgc gctgttcctg tcggcgcgcg ggcgtcgcct
     3061 cgacacgtcg acgttgcgcc gggtgttcgc ccatccgcac agcctgcgcc atgcgttcgc
     3121 aacgcacctg ctggagggcg gcgccgacct gcgcacgatc caggacctcc tcggccacag
     3181 ttcgctctcg actactcagt tgtacagcca tgtagacggc cgccgcctgc gccgcgtcta
     3241 cgacagggcg catccgcgtt cttaaccgag ctacggattc gcgaggatgg cgacgtcgtc
     3301 tgcgtagaag caggtgcctg ggggtgcgtc ggcgctggag aggtaggcgt tgaagtcgag
     3361 cgtcgagccg ggggcggtga tcgtgtagga gacggtcacc tgctgccagc tggtggtgag
     3421 cgtcacctgc gccgtggcgg tgccgagcag ggcgccgctg ctgatcgagt actcgcggaa
     3481 cctgagcttg agcggcttgc cggccgtgtc ggcgcgcacc cagaggctgc ctgtgtaggt
     3541 gccggccgag gtcggcctcg cccagtccgg agagtcgttc agcaggcagg tggtggcagt
     3601 cgtcccgctg ttcgtcagc
//
LOCUS       DASVEU010000110         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_848363,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000110 DASVEU010000000
VERSION     DASVEU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848363"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..803
                     /locus_tag="VF225_04215"
     CDS             405..803
                     /locus_tag="VF225_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7144491.1"
                     /translation="MEQARVDKWLWAARFFKTRSAATQAVLGGRAHVNGVRVKPSHEV
                     RPGDTIEVTMRSEKRTVDIVGIADRRGPAATAAALYVERPESISAREQAAIQRRLAAP
                     PGMGFGGRPTKLDRRRLDALRRAERQKRSS"
     gene            complement(818..1228)
                     /locus_tag="VF225_04220"
     CDS             complement(818..1228)
                     /locus_tag="VF225_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7144492.1"
                     /translation="MATMADLDELALAMPQATKELSDDGRPAYHVHGKLFCFHRGQRR
                     DAIDPDTGERLDDVLMFRVADLGVKELLLADERGIFFTTPHFDGYSAVLMHIPDLARL
                     DRDELEDLVVEAWLTRAQKRVAKAWLDEHRVTGD"
     gene            1298..>2960
                     /locus_tag="VF225_04225"
     CDS             1298..>2960
                     /locus_tag="VF225_04225"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7144493.1"
                     /translation="MSLNAPSPVGVASVRACGPWRLGAVVFAAGAGTLSTEIAASRLL
                     APYFGNSTVVWANIIGLILVYLSLGYWLGGKIADRAPRPELLGGIVLAAAAFIAATPF
                     AARPILDAALRGFDSISVGVVIGSFLAALALFAIPVTLLGAVSPFAIRLSLTDLERSG
                     AVAGRLYALSTVGSIIGTFVSALVAIQAFGTQRTMIGTAALLAGCSVLLIGRRAVPAT
                     IAALGLLLVPAGAVKASSGLLFEKESPYQYVSVVRWDDGTRVLQLNEGVAYHSMWRHG
                     SVLTGQYWDLFGMLPPLLGHPARNMLVIGNAGGTIDRIYGLYYPGTAIDGVELDPAVT
                     EAGRRFLGLDDNPRVHVYTADGRPFLERTRKRYDLIVVDAYRQPYVPFYLATREFFEL
                     ARRHLRRGGILALNVAKVPGDDRLTRAVDTTLLAAFPQVWRWPVLRFNDLVMALDRPV
                     PRSVLVRRAANVRGRLVLLLPAFRHGLVAAHAAGRVLTDDRAPVEWLTDRMLLEQIAR
                     GGGACLYARASASGRAATATGRASGWPRRRGRRSRAGGRAPAPVRS"
BASE COUNT          430 a          997 c          990 g          543 t
ORIGIN      
        1 gagggtgaca tcgtcgaccg cggcgaggcg gcagaagacg ccgagagccg ctagctgcga
       61 gtgagaccga ttttcgtgcg gcgcagtaca ggtttttgaa cgagggccga tctcggctat
      121 tctgccgccg cctaactccg ccgccgagac ctcgcggctc gtttgaaagg ggtccgaatg
      181 ggtgttgagg agttcgacgt gggaagagcg acgggcgacc aaggaccgct gtttcgcttt
      241 cgctgcaccg ggtgttccta cggcgcgagc cggggctcgg cgccggaacg ctgcccgatg
      301 tgtggcggat cgacctggga ctacgacagc tggcgtccct tctcgagcct cgtcggcgac
      361 ctttcgccac ggtcggagcg gtctccgaca gtcaggcagt agcgatggaa caggcgagag
      421 tcgacaagtg gttgtgggcg gcgcgcttct tcaagactcg tagtgcggcg acgcaagccg
      481 tcctcggcgg ccgcgcccat gtgaacggtg tgcgtgtgaa gccgtcgcat gaggttcgcc
      541 cgggagacac catcgaggtg acgatgcgct ccgagaagcg caccgtggac atcgtcggaa
      601 tcgcagacag gcgcggaccg gcggcgaccg ctgcggctct ctacgtcgaa aggccggaat
      661 cgatcagcgc gcgggaacaa gctgcgatcc agcgtcgcct cgcggctccg cccggtatgg
      721 ggttcggcgg tcgtccgacg aagctcgacc ggcgccggct cgatgcactg cgtcgagcag
      781 agcgccagaa gcgctcgtcg tagccgcgcc tttgctccta gtcgcctgtc accctgtgct
      841 cgtcgagcca ggccttcgcg acgcgcttct gcgcgcgggt cagccaggcc tcgaccacca
      901 agtcttccag ctcgtctcga tctagccgcg caaggtccgg gatgtgcatg agcacggcgg
      961 aatagccgtc gaagtgcggc gtcgtgaaga agattccgcg ttcgtcggcg agcagcagct
     1021 ccttcactcc gagatcggcg acgcggaaca tcagcacgtc gtcgagccgc tcgccggtgt
     1081 ccggatcgat cgcatctcga cgttgtccac gatggaaaca gaagagcttg ccgtgcacgt
     1141 ggtacgccgg cctcccgtcg tccgacagct ccttcgtcgc ctggggcatc gccagggcga
     1201 gctcgtctag atctgccatc gtcgccatcg cgccagtctc ccacgccgga gtcggcagcc
     1261 gtctcgactc aagccgggca ggcgtacgct ccaagtcgtg tctttgaacg cgccgagtcc
     1321 ggttggagtg gcaagcgtgc gggcatgcgg tccgtggcga ctcggcgcag tcgtcttcgc
     1381 agccggcgcc ggaacgctct cgacggagat cgccgcctct cgtctgctcg ctccctactt
     1441 cggcaactcg acggtcgtgt gggcgaacat catcgggttg atcctcgtct acctctcgct
     1501 cggctactgg ctcggaggga agatcgccga ccgggctccg agaccggagc tgctcggagg
     1561 gatcgttctc gcggcggccg cgttcatcgc ggcgacgccg ttcgcggcgc gcccgattct
     1621 cgacgctgct ctccgcgggt tcgactcgat ctccgtcggc gtggtcattg ggtcgtttct
     1681 cgccgctctg gcgctgttcg cgattcccgt cacgctgctc ggcgccgtct cgcccttcgc
     1741 gattcggctc tccctcaccg atctcgagcg gtccggcgcc gtcgcaggcc gcctgtacgc
     1801 gctctcgacg gtcggcagca tcatcgggac gttcgtctcc gccctggtgg ccatccaggc
     1861 tttcggcaca caacgaacca tgatcgggac cgcggcgctc cttgccggct gctctgtgtt
     1921 gttgatcggg cgccgggcgg ttcctgcgac catcgccgct cttgggctcc tgctcgtgcc
     1981 agcaggggcg gtcaaagcaa gctccggact gctgttcgaa aaggagtccc catatcaata
     2041 cgtctcggta gtgcggtggg acgacggcac ccgcgtcctg cagctgaacg aaggtgttgc
     2101 ctaccactcc atgtggcgcc atggctccgt actgacgggg cagtactggg acctctttgg
     2161 gatgctgccg ccgctgctag gtcatcccgc gcgcaacatg ctggtgatcg gaaacgccgg
     2221 cggcacgatc gaccgcatct acggcctgta ctacccgggc acagcgatcg acggcgtcga
     2281 actcgaccct gcggtgaccg aggcaggcag gcgtttcctc ggccttgacg acaaccctcg
     2341 tgtccacgtc tacacggccg acggccggcc gttcctcgag cgcactcgca agcggtacga
     2401 cctgatcgtc gtcgatgcct accggcagcc ctacgtgccg ttctatctgg ctacgcgcga
     2461 gttcttcgag cttgctcgcc gccatttgcg gcgcggcgga atcctcgcgc tcaacgtcgc
     2521 caaggtcccc ggggacgacc gcctgacgcg cgccgtcgac acgacgctgc tcgccgcctt
     2581 tcctcaggtc tggcgctggc ccgtattgcg cttcaacgat ctggtgatgg cgctcgaccg
     2641 cccggtcccg cgaagcgtcc tcgtccgtcg cgcagcgaac gtgcgagggc ggctagtgct
     2701 gcttctgccg gcctttaggc atggcctggt ggccgcgcac gcggcgggtc gagtgctcac
     2761 cgacgatcgg gcacccgtcg agtggttgac cgatcggatg ctgctcgagc agatcgcgcg
     2821 aggcggcggg gcttgccttt atgctcgagc atcagcgtct ggccgagcgg cgacggcgac
     2881 tggtcgagca agcggatggc ctcgccgtcg cggccgacgg tcacgagccg gtggccgcgc
     2941 gcccgcgccc gttcgatcac
//
LOCUS       DASVEU010000111        21229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_268303,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000111 DASVEU010000000
VERSION     DASVEU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 21229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21229
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268303"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /gene="nadE"
                     /locus_tag="VF225_04230"
     CDS             complement(<1..1277)
                     /gene="nadE"
                     /locus_tag="VF225_04230"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX7144494.1"
                     /translation="MRLALAQINPVVGDLEGNRALILERLTEAKADSADLVVFPELAV
                     TGYPPEDLLLRPGFIRAAADSVEKIAREARGATVLVGAPYFDRDLFNACYVCSGGEVK
                     AVYRKRFLPNYGVFDEDRYFAPGRDLILLEHGKTLVGLTVCEDMWQPGPPATDLALAG
                     AELLVNISASPFYVGRPDEREEMFVTRARDNSSFVAFCNMVGGQDEPIFDGHSCVLDD
                     EGTVLARAPGFEEALLVVDIEPQEVIGRRLSDVRRRALAREREQIPPVEIIHVGSPGP
                     SGNGSKAVGEVVPFFEEIEQMRRALELGLGDYVGKNSFQEVVVGVSGGIDSAVTAALA
                     AETLGPERVHCVSMPSRYSSEGTRTDARRLAESLGSPFIEIPIGKIVDAFGEALEEAF
                     AGTEPDLAEENIQARARGVLLMALSNKFGWMLVA"
     gene            complement(1329..1874)
                     /locus_tag="VF225_04235"
     CDS             complement(1329..1874)
                     /locus_tag="VF225_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7144495.1"
                     /translation="MGIAVTITAIVFGLAAPSGANSQVSDSSALAAVASSCAGSTTAD
                     GDQHSRLEAMYCGINVVRRAYGLGFVRGSVPLNRSSLLKANAVRRCGFSHTPCGMAFS
                     RTFKQAGYLPARAFGENLAWEQGELGSPWYTLQLWLDSPPHRENLMARRWRDLGIAFE
                     RGHMFGYDGVALWVMQFGQRR"
     gene            complement(2001..3935)
                     /locus_tag="VF225_04240"
     CDS             complement(2001..3935)
                     /locus_tag="VF225_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7144496.1"
                     /translation="MSEFIWSPTAEDVESANVTRLARSLGVERYADLHRISVEEPERF
                     WPAVIEDLGLEFSEPWERVVDTSRGNEWATWFTGGKLNLAWNCVHRWAAGELAEQEAA
                     IWQGEDGTRLSLTWRELSEEVFRLAEGLASIGIGEGDAVGIFLPMSPQVAIASHACAH
                     LGAVQVPIFSGFAPPAIAARLADAKAKALITADGSLRRGQLVPMKEIADEALRSAPTV
                     THTVVWRRLGLDDVPMTLGRDRFWDELVEHASGGLAAKQVDSEHPYLLAYTSGTTGKP
                     KGALHVHAGFLISIAREVAYQTNVKPGDRIHFATDMGWIMGPWTVVGGGACGATIVYA
                     EGAPDFPADRIWRLIESERVTMLGVSPTLVRALIPKGDPQADMSTLKAICTTGEPWNR
                     DPYHWLFEKVGGGNTPIVNCSGGTEVGACFLTTCLTEPIKPVALGFPALGQDMDVFGP
                     DGTPLRGEVGELVCKRPWPGMTRGVWGDDERYLDAYWRRFPGVWTHGDWASIDEDGYW
                     FLHGRSDDTLNIAGKRIGPAELESAAVAHGAVAEAAAIGIPHDVKGEVAWIFCVLKPG
                     SEATPALALEISSNVSDELGKAFKPERIVFVSALPKTRSAKIVRRAVRARVLGKEPGD
                     LSSLENPESLDEIAEAATRA"
     gene            3996..5447
                     /locus_tag="VF225_04245"
     CDS             3996..5447
                     /locus_tag="VF225_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144497.1"
                     /translation="MRARLFTPENRKWWTLVAVAVGLFMIMLDNTVVNVALPSIQQDL
                     GIGISELEWVVNAYALTFGVLLLTGGKLADLLGRRAIFIAGLVVFTGASLWCGLAGGA
                     GSLIAARTVQGIGAALMNPATLSIITATFPPRQRGTAIGIWAGVSALALAIGPIVGGL
                     LTEKVNWSWIFFINIPVGVLGVIAARLFIDETKDTSKEQRLDLPGLVTSGAGLFALTY
                     GLIETNDHAWTSTRVLTLFAIAVVALTAFVLLELRQRLPMLDLSLFRNPTFAGANAAM
                     LLVGLAMFGIFFYNSLLLQRVLGYSAIETGATFLPMTVLIILVAPIAGRFSDRIGARW
                     LMGAGMTLLAVSLLLFGTLDANSSFWNILPGLLVGGLGMAVTMAPTTAAAMGSVPVAK
                     AGVGSAVINSMRQVGGSLGIAIMGALVATSVSVLPTSPLWPDQFVSGYHRAVHLGAAL
                     VLVGAIISVLTVRQLPRREGAAESVVGALANEP"
     gene            5781..7133
                     /locus_tag="VF225_04250"
     CDS             5781..7133
                     /locus_tag="VF225_04250"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7144498.1"
                     /translation="MSDSTAIRQQQRASAAQAGLRLGERLRQLRVAGGMTQTDLAGDR
                     FSKEYVSQIERGKTRPTRETIDWLAQRLEVDSGFLANGVSADERGRVDAALTRAGALL
                     EARRNDEALEEFESIRSAVLATGMPELEARALSGEATVRMRAGDVREAIALLERARAL
                     SEGTSFSDVERADVLFRLGVARYKLNSIQTSIGLFDEALKVAERSEIPSDQLRSNILA
                     WRSRCYRRRRDLEAAREDVERALELAEGLNDKRTAADIYFQASIIADREGHWVLARSY
                     AERAKAAYEELSDRGNLGRLLNNLGGINFLLGHPEEAVDFLKDAVGIALEVGNDAEAA
                     HAVNGIAQVHLRTGDVKRAEEQARYALELLGDRVDEIAEIGNAHLVLGRALLEQDRLD
                     EADEALQAGERAYDQLSSGSHRASAWVAQGDLAARRGDDRAASRLYRQAADALQDVRF
                     "
     gene            7482..7688
                     /locus_tag="VF225_04255"
     CDS             7482..7688
                     /locus_tag="VF225_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144499.1"
                     /translation="MSATGEEPGLVSVREVADQLGVHPETIRRLIHDGRLDAVRVGRV
                     LRVHREAVEHFLARQRVKPTRFQA"
     gene            complement(7700..8398)
                     /locus_tag="VF225_04260"
     CDS             complement(7700..8398)
                     /locus_tag="VF225_04260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7144500.1"
                     /translation="MEKAALVTGGSSGIGLAIAKMLRENGFALTLAARTPEKVEAAAE
                     TLGAHAVTADVADAEDCRRLVEEHRTQHGRLDVLVNSAGVGIAGPIDELATKHIDLQL
                     NVNLRGTLLVTAAALPMLRESHGLIVNLASIAGTSPSSHLPVYGAAKAAVINFTSALN
                     RGEEKHGVRATALSPGFVDTPMTSWAPVPPEQMIRPEDCAEVVQALLRLSPHARLPHV
                     VIERVSTEESVFGA"
     gene            8647..9684
                     /gene="selD"
                     /locus_tag="VF225_04265"
     CDS             8647..9684
                     /gene="selD"
                     /locus_tag="VF225_04265"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX7144501.1"
                     /translation="MDTDTGRVKLTDLASCGGCAAKYSAARLEQLLAGFVPAEAENLL
                     VGLAPADDAAVYRLDDERALIFTLDFFPPVVDDPGDYGAIAATNALNDVFAMGGTPLL
                     ALSIAAFPEELPIEMLAEIFAAADAQVRAAGGLLAGGHTIRDEEPKYGLAVVGTVRPD
                     GIWPKNGAKPGDALFLTKPLGTGLIMTGYKKGLAGDMQLERAIRWMRTLNKDAAEVLR
                     RLEPNAVTDVTGFGLFGHAHEVADRSGVGIWLESERFPAIDGALDVARKGVRTSGDPR
                     NRDFAAAHVTTDGLSETLGALGYDPQTAGGLLVSLPADQGAALEAEFAERRLFVRRIG
                     RVEEGAGVVVE"
     gene            9693..10199
                     /locus_tag="VF225_04270"
     CDS             9693..10199
                     /locus_tag="VF225_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX7144502.1"
                     /translation="MIHGRFQPFHNGHLEYLRGAAAQSDEVFVGITNPDPQRIKEEPS
                     DPLRHLPESNPFTYVERLLMIEAVAEDERIRAHVIPFPVNEPELWSAYVPAGVTQYLR
                     LFSEWGGTKLERMRDAGYQVVVLDEGVDKEISGADVRAALRSGADWEAFVPPGVARVI
                     RSLERVPV"
     gene            10200..11444
                     /locus_tag="VF225_04275"
     CDS             10200..11444
                     /locus_tag="VF225_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7144503.1"
                     /translation="MHPIVVFLLDGLADRAHESLGGRSGLEAATTPNLDRLCSAGSCG
                     VLYAIGPGRAPSSEVAHWSMFGYRPEEFPGRAVFEALGRWQEVSPEHVFAYAALRPAE
                     RREDGWWLTGRPDPDQDAEEAERLVEACNGITVDGLTFSLAHVWRGEAVLRLSGGADE
                     RVTDSDAFFRDRHPVLRPQALVPEAERTARAAEEWSREVMRRLEGERFNVITLKWWGR
                     PRDVPTFEQRHGLRGTFIASSAFLRGLARAVGLAAREEAETDDAAADLRGRVALARER
                     LEAGDTFVFCHVKSADAAGHTKDPREKRAMIETLDAALVDLPTNRAIVCLTGDHATPA
                     SPDVIHSGDPVPFVVAGPGVRADRVGEFGESTCAEGILGHLRGPDMMPVLLNAADRPL
                     FLGSRPTPFDGADGYPAILDPL"
     gene            11459..12394
                     /locus_tag="VF225_04280"
     CDS             11459..12394
                     /locus_tag="VF225_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7144504.1"
                     /translation="MHAQPTALARLRALALTPAQIYPLVLVQLGALWLIIGTGAAVRL
                     TDSGLGCRHWPGCEAGHPLPAKNAHAFVEFGNRLVGGVTIAVTLLAWLAVRRVPGLPR
                     FLRRLALAVFLGTLAQAPIGLLAIATDLRWPIVMVHLLLSIALLGGATVLVLEVRSAT
                     VGRIEPLVPPELQRLGLAFVCAAFVLIVSGSLATASGPHSGGGAADKIGRVGRLAPFV
                     YAHATTVGIFLLTFIFSLGYLAARRDRSPRLFKLGAAVFVLLLVQMGLGELQWRTHLP
                     WGLVLAHVFLAATVWVGTVALATVFVRPNVDFSDR"
     gene            12441..13283
                     /locus_tag="VF225_04285"
     CDS             12441..13283
                     /locus_tag="VF225_04285"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7144505.1"
                     /translation="MAAFRGWNDGGQGASLAGGYLAKIWEADRFADIEPENFFDFQAT
                     RPHVSLVDGQTRHIDWPENAFYHAPIPDLDRDAVLLLGIEPNLRWQTFTGMIVDLARE
                     LGVQMMITLGSLLADVPHTRPSPVTGGGTDPELVERLGLQRSRYEGPTGIVGVLHDAC
                     NHASIPSVSLWAAVPHYVSLAPSPRAALALCQRLGDILGTQIDTAELQEASERYSEQV
                     TEAVASDEETQAYVEELEQRAELLEDEEDLPSGESLAAELTRFLREREHGNGGPERGP
                     SEQP"
     gene            complement(13289..14956)
                     /locus_tag="VF225_04290"
     CDS             complement(13289..14956)
                     /locus_tag="VF225_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEX7144506.1"
                     /translation="MLILVPIAFAAGVITAFTPCILPVLPIVLAGGAGGGTKRRPYAI
                     AAGLVTTFTAFLLAGAWLWGLLGISAKHQIQIGAALLLLLALTLIVPKVGEQVERPFA
                     LLTRRRAGDLGGGFFLGASLGLVFVPCGGPVLASLTANIATNRVGGWIVAIAIAYAVG
                     AALPLLAIAHGSRGLAARFRRHAQAVRIGGGVLMAAAAVVIYQGWAETLQMKVPGYAQ
                     RVQNAIEGTATAKERLDRLDGKAKPAFVAHGQRLASQLGKVPLKDYGAAPDVHDVSAW
                     INSRPLSLPKLRGKVVLVDFWTYSCINCLRTLPYLKAWDARYRSKGLVILGVHTPEFA
                     FEHDLGNVRAAVKRLGVDYPVALDNSYGTWKAYSNQYWPADYLVDQAGRVRDVHFGEG
                     DYAQTERNIRLLLAAGKAGRLPQAGRDTDRTPLELRTPESYLGYLRIGNYTGSPLRTD
                     RVATYRFPASLTQDSYAYAGRWKVEGERIVAEDNARLRVHFQARTVHLVLTGRGFVAV
                     KVNGKTQPTVRVDGDRLYTLVSQKSAADGALELSFTRGLAAYAFTFG"
     gene            complement(14972..17554)
                     /locus_tag="VF225_04295"
     CDS             complement(14972..17554)
                     /locus_tag="VF225_04295"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7144507.1"
                     /translation="MQEIKEQPTTASEATDGLVADALRAEAWAGEPFIREGEDPAKVL
                     APGTSRRRALDDALAEIEGGQRTPSSGWKVEYGFMLGLERVLAEEKPHLASGTELRRH
                     QIDALAGMLTELIASHEKEAGPNGNGNGNGVAEVVEAEVEVEEDDDEPAEESAEDVLD
                     EEVEEDEHYTGPDPGAVRRYRFRHPTASGKTIAAAGFVEAARSLGVLILTHRRLLVSQ
                     FQRDLTDEGYGDRFAPAVERGKESLQNGNPLTIQTYAWFARHQETISREAYQLVICDE
                     AHTALGEKTSAAIRAFPEPIYIGMTATEQLIAKQVSDVFPASVDDLPLQDAARRGLIA
                     PLRDLRVPPVAAINQVPIVGGDFDQEILAKTLDHQALNQAAASLYRERFDNTPGIVYA
                     AGVDHAYNLAKEFRAVGLKAEAVSGRTPPVRLAETLAAYERGEINVLINAMLLAEGWN
                     SPRATICMHLAPTASRRVYQQRIGRIMRTHPRKEAGIVVDFVPKAATHNERVISLHSL
                     LDADFYREGARVTPAPRRRVQRRARRKLTPAPWLVPVTPDVRRRLAVITREWQRVDPK
                     YLDEDEQRYWATIAGRQIRFDERAAFVEKLTARGASKACLEQFLATCAAENPNRRLRM
                     IALSDRVSMTVERADFDDLVTLVTQAPTWEKDRMGGVRVLLRAIAEGKADAPDQILAR
                     WTWRLARASRKVQDRRASAEFPEAKRLLGALANSRGHRHEENAAKLVNVALELPMPVG
                     AALLASAEGYTPRATQLIDAARERLGTVHEVAGALAENLPQPKLSSGRSRRRKRRKRK
                     KSGTQQPQTQGQQQQQQQQQQAKAPEGQEQQVAAAEAQKPKPRPRKKPAPKPEPASAE
                     TPAA"
     gene            complement(17676..18263)
                     /locus_tag="VF225_04300"
     CDS             complement(17676..18263)
                     /locus_tag="VF225_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144508.1"
                     /translation="MAHDDWRIRIELPDEADAQDLLGRLGLRQSGAEELADDLREHRL
                     AVSQDGDTVFVYAASGMQAEQAARIIESELEDQGLTPSRFVTERWLQDEERLNDDPEQ
                     PDIEEELLQRGYAPWEVRVEADSLREAHDLAEQLRSEGYDVSRTFTYVIAGARSREEA
                     VELARRIHGEVEPGGELVYEVQPQNPFAVFGGLAG"
     gene            complement(18421..20394)
                     /locus_tag="VF225_04305"
     CDS             complement(18421..20394)
                     /locus_tag="VF225_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7144509.1"
                     /translation="MAPVTEFKLTRAGDVALVTIDNGEDWTKPTFFGQEALESLDRLL
                     GRLEESDFRAAVITGKPFVFAAGADITEFPDITRERAIAGSRAGHELFGRLRALPFPT
                     VAAINGACLGGGVELALHCSARTISTAVRHFAFPEVFLGLFPAWGGTQLLPRLVGPET
                     AIKVIVSNPLRQNRMLTGPQVAELGIADRLLEPVEFVDESLAFARELADQLLERPEPD
                     WSDAETIFRRARTAVDDTVHGAAPAPYAALDLIAGAQEWTIDEGYAREREAIGALLPG
                     PQAQASLYAFQLVELRAKRHPARPTVPPLKVNRVGIVGAGLMARQIATLLLRRLEVPI
                     VIRDVKQEIVDAALADIRAGIDSQVAKGRYDEGKGRFLASLVSGSTDYEGFADCDLVL
                     EAVFEELEIKQQVFAELEQVVRDECVLATNTSALSITDMAADLRRPERVAGMHFFNPV
                     ALMPLLEVVRAAETDEVTLATVWDAGEKLRKRPILVNDAPGFVVNRVLTRMTRVIMDA
                     VEHGTPVEEVDEAVMSLGMPMAPSVLLQMVGPRVANHVLERMHDAFPERFPLSPALAA
                     IADGNEPVVLEDAPRTREQIVEDVLEAVADEIHLILEEGVVTEAADVDTGLLLGAGWP
                     FFLGGITKHLDQIGISERLFGHKFAELTAVARA"
     gene            complement(20378..>21229)
                     /locus_tag="VF225_04310"
     CDS             complement(20378..>21229)
                     /locus_tag="VF225_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7144510.1"
                     /translation="CDIVLAGGVEHMGHHPMGEDVDFNPRFVSERLVDPSAVTMGATA
                     ENLHDRFPQITKHDADAFALQSQQRAAAAWSNGVMRETVVPMSVFTDDGWKLADSDEF
                     LRSDTTLEGLATLRTPFRAGGRVTAGNSAGLTDGATACFLAAEETAAELGLEPKMRLV
                     AFGFAGVEPELMGIGPIPATKRVLEQAGLALDDVGLFELNEPFAVQVLTWCEGMGIDP
                     KDPRLNPYGGAIACGHPLAATGVRLMAQLAYGFRERPDVRYGLTALCVGMGMGAALLW
                     ENLGAGH"
BASE COUNT         3267 a         7202 c         7158 g         3602 t
ORIGIN      
        1 gcgacgagca tccagccgaa cttgttcgac agtgccatca gcaggacgcc gcgagcccgc
       61 gcctggatgt tctcctccgc gaggtcgggc tcggtcccgg cgaacgcttc ctcgagtgcc
      121 tctccgaagg cgtcgacgat cttgccgatc gggatctcga tgaacggaga gccgaggctc
      181 tcggcaagcc ggcgggcgtc cgtgcgcgtg ccctccgagg agtagcgcga cggcatcgag
      241 acgcagtgca cgcgctccgg cccgagtgtt tccgccgcga gggcggcggt caccgcggag
      301 tcgattcctc ccgacacgcc gaccaccacc tcctggaaac tgttcttgcc gacgtaatct
      361 cccagcccga gctcgagtgc acgccgcatc tgctcgatct cctcgaagaa cgggacgacc
      421 tcgccgacgg ccttgctgcc gttgccggac ggccccggcg agccgacgtg gatgatctcg
      481 accggcggga tctgctcacg ctcgcgtgca agggcgcgac gacgcacgtc gctcagacgc
      541 cggccgatca cctcctgcgg ctcgatgtcg acgacgagga gcgcttcctc gaaaccgggt
      601 gcgcgtgcga gcacggttcc ttcgtcgtcg agcacgcacg agtggccgtc gaagatcggc
      661 tcgtcctgtc cgccgaccat gttgcagaac gcgacgaagg acgagttgtc ccgcgctcgc
      721 gtgacgaaca tctcctctcg ctcgtcggga cgtccgacgt agaaaggcga tgccgagatg
      781 ttcaccagca gctcggcccc ggccagtgcg agatccgtcg cgggcgggcc cggctgccac
      841 atgtcctcgc agaccgtcag gcccaccagg gtcttgccgt gctccagcag gatcaggtca
      901 cggccgggcg cgaagtagcg gtcttcgtcg aagacaccgt agttcggcag gaagcgcttg
      961 cggtagaccg ccttgacctc gccgcccgag cagacgtaac aggcgttgaa gaggtcacgg
     1021 tcgaagtaag gggcgccgac gagaacggtg gcgccccgcg cctctctggc gatcttctcg
     1081 accgagtccg cggcggcacg gatgaaaccg gggcggagca gcaggtcctc gggcggataa
     1141 cccgtgaccg ccaattcagg gaagacgacg aggtccgcgg agtcggcctt cgcctccgtg
     1201 agccgctcga ggatcagcgc gcggttcccc tcgaggtcgc cgacaaccgg gttgatctgg
     1261 gccaatgcga gccgcacagc cccagtctag ctgtttgcgc ctctcaggcg aaaactaaca
     1321 acgtggcctc agcgacgctg accgaactgc atcacccaga gtgcaacgcc gtcgtagccg
     1381 aacatgtgcc cgcgctcgaa cgcgatcccg aggtctcgcc agcggcgtgc catcaggttc
     1441 tcgcgatggg gcggggaatc gagccagagt tggagcgtgt accacgggga gccgagttct
     1501 ccctgttccc acgcgagatt ctcgccgaac gcgcgggccg gaaggtagcc ggcttgcttg
     1561 aacgtccgag agaacgccat cccgcagggc gtgtgcgaga agccgcaccg ccgcactgcg
     1621 ttggctttca gcagcgacga gcggttcagc gggacgctgc ccctgacgaa accgagcccg
     1681 tacgcgcggc gcacgacatt gatgccgcag tacatcgcct cgaggcgcga gtgctgatcg
     1741 ccgtcggcgg tcgttgaacc ggcgcacgac gacgcaacgg ctgccagcgc ggaggaatcg
     1801 gagacttgag aatttgctcc cgatggcgcg gccaagccaa acacgatcgc ggtgatggtg
     1861 acggcaatcc ccatgcgcgg tcgtcagcct gttacccctt cgggccggat cctgcccctt
     1921 tttgcagcga aatttacatt caggttcgtt tctcgcacgc atatgcgtcg agaaacgtat
     1981 gagctgagaa acggaaacgt tcaggcgcgg gtcgcagctt cggcgatctc gtcgagggac
     2041 tccgggttct ccagcgaaga cagatcgccc ggttccttgc ccaggacgcg cgcccgcacg
     2101 gcccgccgga cgatctttgc gctgcgggtc tttggcagcg ccgagacgaa aacgatccgt
     2161 tccggcttga aggctttccc gagctcgtcg ctgacattgc tcgaaatctc gagcgcgagg
     2221 gccggcgtcg cctcgctgcc gggcttgagc acgcagaaga tccacgcgac ctcgcccttc
     2281 acgtcgtggg gaatcccgat cgcagcggcc tcggccaccg cgccgtgcgc gaccgccgcc
     2341 gactcgagct cggccgggcc gattcgcttg cccgcgatgt tgagcgtgtc gtccgagcgt
     2401 ccgtgcagga accagtagcc gtcttcgtcg atcgatgccc agtcgccgtg tgtccagact
     2461 cccggaaagc gtcgccagta cgcgtcgagg taacgctcgt catcgcccca gacgccgcgc
     2521 gtcattccgg gccacggccg cttgcagacg agttcgccga cctcgccgcg caacggcgta
     2581 ccgtccggtc cgaacacgtc catgtcctga ccgagcgccg ggaagccgag cgcgaccggc
     2641 ttgatgggct cggtcaagca ggtcgtgagg aaacacgcgc cgacctcggt gccgccggaa
     2701 cagttgacga tgggcgtatt gccgccgccg actttctcga agagccagtg atacggatcg
     2761 cggttccacg gttcgcccgt cgtgcagatc gccttcaagg tggacatgtc tgcctgcggg
     2821 tctcctttgg ggatcaaggc ccgaaccagg gtcggggaga cgccgagcat cgtcacgcgc
     2881 tccgactcga tcagcctcca gatccggtca gccggaaagt ccggtgcgcc ttcggcgtac
     2941 acgatcgtcg caccgcacgc cccgccgccg accacagtcc acggacccat gatccaaccc
     3001 atgtccgtcg cgaagtggat gcggtcgccg ggcttgacgt tcgtttgata ggcgacctcg
     3061 cgcgcgatcg agatgaggaa gcccgcgtgg acgtgcaacg cccctttcgg ctttccggtc
     3121 gtaccggagg tgtatgcgag caggtaggga tgttccgaat cgacctgttt cgccgccagg
     3181 ccgccggacg cgtgctcgac gagctcgtcc cagaagcgat cgcgcccgag ggtcatcggc
     3241 acgtcgtcga ggccgaggcg ccgccagacg accgtgtgcg tgaccgtcgg cgcggaccgg
     3301 agcgcctcgt cggcgatctc cttcatcggc accagctgcc cgcgacggag ggacccgtcg
     3361 gccgtgatca gcgccttggc tttcgcgtcc gcgagccgtg cggcgattgc cggcggcgcg
     3421 aagccggaga agatcgggac ctgaacggcg ccgagatgcg cacaggcatg tgacgcgatg
     3481 gccacctgag gcgacatggg caggaagatc ccgaccgcgt cgccctcgcc aatgccgatc
     3541 gaggccaggc cttcggcgag ccggaagacc tcctccgaca gctcgcgcca ggtcagggag
     3601 aggcgggttc cgtcctcgcc ctgccagatc gcggcctcct gctccgccag ctcgccggcg
     3661 gcccacctgt gaacgcagtt ccaggcgaga ttcagcttgc cgccggtgaa ccacgtggcc
     3721 cactcgtttc ctcgagacgt gtcaaccacc cgttcccagg gctcggagaa ctcgagcccg
     3781 agatcctcga tcacggcagg ccagaagcgc tcgggctcct cgaccgaaat gcggtggaga
     3841 tctgcgtaac gctccacccc gaggctccgc gcgagcctcg tcacattcgc gctctcgacg
     3901 tcctcagcag tcgggctcca gatgaactcg gacacgcgcg aagcgtacgg gtagccggac
     3961 cacgtagggc gggcctgttg ctctaacctt ttgggatgcg tgctcggctg ttcacgccgg
     4021 agaaccgcaa gtggtggacg ctcgttgcgg tcgcggtcgg cctgttcatg atcatgctcg
     4081 acaacacggt cgtgaacgtg gcgctgccgt ccattcagca ggacctcggg atcggcatct
     4141 cggagctcga atgggtcgtg aacgcctacg cgctcacgtt cggcgtgctc cttctcaccg
     4201 gcggcaagct tgccgacctg ctcggccgcc gggcgatctt catcgcgggc ctcgtggtct
     4261 tcaccggtgc gtcactgtgg tgcggcctcg ccggcggcgc ggggtcgctc atcgccgcgc
     4321 gcacggtgca gggcataggc gcggcgttga tgaatcccgc gacgctgtcg atcatcaccg
     4381 cgacgtttcc gccacgccag cggggcaccg cgatcggcat ctgggccggc gtctctgcgc
     4441 tcgcgcttgc gatcggcccg atcgtcggcg gcctgctgac ggagaaggtc aactggagct
     4501 ggatcttctt catcaacatt ccggtcggcg tgctcggcgt gatcgcggca agactcttca
     4561 tcgacgagac gaaggacacg tcgaaggaac agcggctcga cttacccgga ctggtcacct
     4621 cgggggccgg cctcttcgcc ctcacctacg gactgatcga gaccaacgat cacgcgtgga
     4681 cgtcgacacg tgtgctgaca ctgttcgcaa tcgcagtggt ggccctgacc gccttcgtgc
     4741 tgctcgagct gcgccagcgt ctgccgatgc tcgacctctc gctgttccgg aatccgacgt
     4801 tcgccggcgc caacgccgcg atgctgctcg tcgggctcgc gatgttcggc atcttcttct
     4861 acaactctct tttgctacag cgcgtcctcg gctacagcgc gatcgagacc ggcgcgacgt
     4921 tcctcccgat gaccgtactg atcattctgg tcgctccgat cgcaggcagg ttctccgacc
     4981 ggatcggcgc gcgttggctg atgggggcgg ggatgaccct gctcgcggtc tcgctgcttc
     5041 tcttcgggac gctcgacgcc aactcgtctt tctggaacat cctgccggga ctgctcgtgg
     5101 gcggcctcgg gatggcggtc acgatggcac ccacgacggc tgcggcgatg ggatccgttc
     5161 ccgtggcgaa ggcgggcgtg ggctccgcgg tgatcaacag catgcgccag gtcggcggct
     5221 cgctcgggat cgcgatcatg ggcgcgctcg tggcaacgag cgtcagcgtc ctgccgacga
     5281 gtccgctctg gccagaccag ttcgtgagcg gctaccaccg agcagtccac ctcggtgccg
     5341 ccctcgtcct cgtcggcgcg atcatttctg tgctgacggt tcgccagctg ccccgccgcg
     5401 agggcgcggc ggaatcggtc gtcggcgccc tggcaaacga gccctgaaag gaggaagacc
     5461 cccggctgcg gctccggggg tcgattcctg ttaggcgact ccgccctgtg tggcagtctc
     5521 cccgggactt ggctctgcgg cccagccacg cccctttcgg atcccacctg gtgaagtgcc
     5581 tcctgcagcc gaccctggcg tctggcccgg atgcaagatg gcctcactgt aggaggcttc
     5641 tccgggcatg tcaagtagct cggtcaagtg atttgtatta ggcttacttg actggtttct
     5701 cgcccagaca tgaccttttc gggggatgtt gaaaaggaca ggttgaagtt gggtgaagca
     5761 tgtcgtctac tacccaatac gtgtctgact cgaccgcaat ccgtcagcaa cagcgggcct
     5821 ccgcagctca agccgggctg aggctcggcg agcgcctgcg ccagctccgc gtggccggcg
     5881 gaatgaccca gacggatttg gcaggcgacc ggttctcgaa ggagtacgtc agtcagatcg
     5941 agcgcggcaa gacgcggccc acgcgcgaga cgatcgactg gctcgcgcag cgcctcgagg
     6001 tggactcggg ctttctcgcc aacggtgtct ccgcggacga gcgcggacgt gtcgatgcag
     6061 ctctgacgcg cgccggagca ctcctcgagg cgcgccggaa cgacgaggcg ctcgaggagt
     6121 tcgagagcat tcgctccgcc gtgctggcca ccggcatgcc tgagctcgaa gcgcgcgcgc
     6181 tctccggtga agcgacggta cggatgcgcg caggcgacgt ccgcgaggcg atcgcactgc
     6241 tcgagcgggc ccgcgccctc agcgagggaa catccttctc cgacgtcgag cgcgccgacg
     6301 tgctcttccg gctcggcgtc gcccgctaca agctcaacag catccagacg tcgatcgggt
     6361 tgttcgacga ggcgctcaag gtcgccgaac gctcggagat tccgtccgat cagctgcgct
     6421 ccaacatcct tgcgtggcgc tcgcgctgtt atcgccggcg tcgcgacctg gaagccgcgc
     6481 gcgaggacgt cgaacgcgca ctcgaactgg ccgagggcct gaacgacaag cgcacggccg
     6541 ccgacatcta cttccaggct tcgatcatcg cggatcgcga aggtcactgg gttctcgcgc
     6601 gttcctatgc cgagcgcgcc aaggccgcct acgaggagct gtcggaccgc ggcaatctcg
     6661 gccgcttgct caacaacctc ggcgggatca acttcctcct cggccatccc gaggaggccg
     6721 tggacttcct caaggacgcg gtcggcatcg cgctcgaggt cggcaacgac gcggaggccg
     6781 cacacgcagt caacggcatc gcccaggtcc atctgcgcac cggcgacgtc aagcgggccg
     6841 aggagcaggc gcgctacgcg ctcgagctgc tcggcgaccg cgtcgacgag atcgccgaga
     6901 tcggcaacgc ccatctcgtc ctgggccggg cgttgctcga gcaggaccgg ctcgacgagg
     6961 ccgacgaggc gctccaggcc ggcgagcgtg catacgacca gctttcttcc ggaagtcatc
     7021 gcgcatcggc ctgggtcgcc cagggcgatc tcgccgcccg gcgcggcgat gaccgggctg
     7081 cctcccggct ctatcgccag gctgccgacg ccctgcaaga cgtccgcttc taagccagct
     7141 ctcgagagga ggtgaccacc gtgtccaaag ctcgcctgat ctattttgcg gtcttcgcga
     7201 tcatcatcgt gacagccctg ctgccggcgc tgagtctgtg gccagaaggg ccgcacgacg
     7261 gcgcagagat ctaaagtgaa cgtgcgcgcg ctttgctcgt ggccctcgcg ctgatggctt
     7321 tggcggcagg ctgtggctct acgacgagtc gttcggagcg aagcgcgccc agccccgggt
     7381 cgctggaggc acaccttgtg cagtcgcccg ccaatcctcg acgcctggtt cggagggctg
     7441 atgcgcgagg ccgaagaacg tgcaaggctt ggaccacgac cgtgtctgca acaggcgaag
     7501 aacccggact cgtcagtgtt cgcgaggttg cggatcagct cggcgtgcac ccggaaacga
     7561 tccggcggct gatccacgac gggaggctgg atgcggtgcg tgtcggccgc gtcctccgcg
     7621 ttcacaggga agcggtcgag cactttcttg cgcggcaacg cgtcaaaccg acgcgtttcc
     7681 aagcctagac ggcgggacgc taagcgccga agacagactc ttcggtgctg acgcgctcga
     7741 tcacgacgtg tggcagccgc gcatgggggc tgagccgcag cagcgcctgc acgacctcgg
     7801 cgcagtcttc cggacggatc atctgttccg gagggaccgg ggcccaggag gtcatcggcg
     7861 tgtcgacgaa gccggggctc agcgcagtcg cgcgaacgcc gtgtttttcc tcgcctctgt
     7921 tgagggcgct cgtgaagttg atcacggccg ccttggccgc gccgtagacg ggcaggtgcg
     7981 aggaagggct cgtccccgcg atcgacgcga ggttcacgat caagccgtgg ctctcgcgca
     8041 acatcggcag tgccgcggca gtgaccaaga gcgttccgcg gaggttgacg ttcaattgca
     8101 ggtcgatgtg cttcgtcgcg agctcgtcga tgggaccggc gatccccacg ccggcggagt
     8161 tcacgagtac gtcgagccgc ccgtgttggg tgcgatgctc ctcgacgagg cgacggcagt
     8221 cctctgcgtc cgcaacgtcg gctgtgacgg catgggcgcc gagggtctcg gccgccgcct
     8281 cgaccttctc aggggtcctg gcggcgagcg tgagggcgaa gccgttctcc cgcagcatct
     8341 tcgcgatggc aaggccgata cctgaagagc ctcccgtgac gagcgccgcc ttttccacca
     8401 aaccatcttt ccaagtcgga aggtgcagcg ccggccgtgc cgaactgggc ggcgggggaa
     8461 ccgaaaggct cctcctctcc cctgaaagcc acgggggaac cacatggttc ctccgtggcc
     8521 ttttctcttc cggcaggcgg gaatagaacc gggcaccggg tcgtaacaag gtgaaccgac
     8581 ggcagtctgt agacgaaagg ctcacagacc cctacatttc gcggttcaac ccccgggagg
     8641 gcttctttgg acaccgacac agggcgcgtc aagctgacag atctcgcctc gtgcggcggt
     8701 tgcgctgcga agtattcggc ggctcgtctc gagcagctgc tcgcgggctt cgtcccggcg
     8761 gaggcggaga acctgctcgt tggactggct cccgccgacg acgcggcggt gtatcggttg
     8821 gacgacgagc gggcgctcat cttcacgctc gacttcttcc cgccggtggt ggacgatccc
     8881 ggcgactacg gcgcgatcgc ggcgacgaat gcgctgaacg acgtctttgc aatgggaggc
     8941 acgccgttgc ttgctctctc catcgctgcg ttccccgagg agctgccgat cgagatgctc
     9001 gcggagatct tcgccgctgc ggacgcgcag gtgcgcgccg ccggcgggct gctggcaggg
     9061 ggtcacacga ttcgggatga ggagcccaag tacgggctcg cggttgtcgg cactgttcgt
     9121 cccgacggga tctggccgaa gaacggcgcg aagcccggcg atgcgctctt cttgacgaag
     9181 ccgctcggaa ccgggctgat catgaccggt tacaagaagg gcctggccgg cgacatgcag
     9241 ctcgagcgtg ccatccgctg gatgcgaacg ctgaacaagg acgctgccga ggtcctgcgg
     9301 cgcctcgagc ccaacgccgt cacggatgtc acgggcttcg gtctgttcgg gcacgcacac
     9361 gaggtcgcgg atcgcagcgg cgtgggtatc tggctcgagt cggagcggtt cccggccatc
     9421 gacggagctc tcgacgtcgc gcgcaaaggc gttcgaacga gcggcgatcc tcgcaaccgt
     9481 gacttcgcgg cggcgcacgt gacgaccgac ggtctctcgg agacgctcgg cgcgctcggc
     9541 tacgacccgc agacagcggg aggcctgctc gtctcccttc cggcggatca gggcgccgcg
     9601 ctcgaggccg agttcgccga acgcaggctc ttcgttcgca ggatcggtcg cgtggaggag
     9661 ggcgccggcg tcgtcgtcga gtgaacgccg gaatgatcca cgggcgcttt cagcccttcc
     9721 ataacggtca cctcgaatat ctgcggggag cggccgcgca gtccgacgag gtgttcgtcg
     9781 ggatcacgaa tccggatccg cagcgcatca aggaagagcc gagcgatccc ctgcgccacc
     9841 tgcccgaatc gaatccgttc acgtacgtcg agcgcctgct gatgatcgag gccgtcgcgg
     9901 aggacgagcg cattcgcgcg cacgtgatcc ccttcccggt gaacgagccg gagctgtggt
     9961 cggcgtacgt gcctgcgggc gtgacgcaat acctgcggct gttctcggag tggggcggca
    10021 cgaagctcga gcggatgcgt gacgccgggt accaggtggt ggtgctcgac gaaggggtgg
    10081 acaaggagat ttccggggcc gatgtgcggg ccgcactgcg ttcaggcgcg gactgggagg
    10141 cattcgttcc tccgggtgtc gcacgcgtga tccggtcgct cgagcgcgtc ccggtctgaa
    10201 tgcatccgat cgtcgtcttt ctgctggacg ggcttgccga ccgcgctcac gagtcgcttg
    10261 gcggccgcag tgggctcgag gctgcgacga ccccgaatct cgaccgcctc tgctccgcgg
    10321 gctcctgtgg cgtcctctac gcgatcgggc cgggacgggc gccctcgagc gaggtcgcgc
    10381 actggtcgat gttcggttac cggccggagg agtttcccgg gcgtgccgtc ttcgaggctc
    10441 tcggccgctg gcaggaggta agcccggagc acgtcttcgc gtacgcggcg ctgcggccgg
    10501 ccgagcgccg tgaggacggc tggtggctca ccgggcggcc cgatcccgac caggacgcgg
    10561 aggaagccga gcgacttgtc gaggcgtgca acggaatcac ggtcgacggc ctgacgttct
    10621 cgcttgcaca cgtctggcgg ggtgaggcag tccttcgcct ctccggaggt gcggacgagc
    10681 gcgtgaccga cagcgacgcg ttcttccgcg atcgccatcc cgtgctgcgt cctcaggcgt
    10741 tggtccccga agcggagcga accgcgcgtg cggcggaaga atggagccgt gaggtgatgc
    10801 gacggctcga gggcgagcgc ttcaacgtga tcacgctgaa gtggtggggc aggccgcgtg
    10861 acgtcccgac gttcgagcag cggcacgggt tgcgcgggac gttcatcgca agctctgcgt
    10921 tcctgcgggg gctggcgcgc gctgtcggtc tcgccgcacg cgaagaggcc gagacggacg
    10981 atgcagcggc cgacctgcgc ggcagggtcg cgctcgcacg ggagcgactc gaggcaggcg
    11041 acacgttcgt gttctgccac gtcaagagcg cagatgctgc agggcacacg aaagaccctc
    11101 gagagaagcg ggcgatgatc gagacactcg acgcagcact cgtggatttg ccgaccaacc
    11161 gcgcgatcgt gtgcttgacc ggcgatcacg cgacgcccgc gtccccggac gtgatccact
    11221 ccggcgatcc tgtgcccttc gtcgtcgccg gtcccggagt tcgtgcggat cgcgtcggcg
    11281 agttcggcga gtccacgtgc gcggagggga ttctcggcca cctgcgcggg ccagacatga
    11341 tgccggtgct gctgaacgcc gccgaccgcc cgctcttcct cggctcgcgt cccaccccgt
    11401 tcgacggcgc ggacggctac cccgcaattc tcgatcccct ctgaatagac tcgaagccgt
    11461 gcacgcccag cccacggcgc tcgctcggct tcgggcgctc gctctcactc ctgcacagat
    11521 ctacccgctc gtgctcgtgc aactcggcgc gctgtggctc atcatcggca ccggagccgc
    11581 ggtgcggctg accgactccg gtctcggctg ccggcactgg cccggctgcg aagcgggaca
    11641 tccgctgccg gccaagaacg cgcacgcgtt cgtcgagttc ggcaaccggc tcgtcggcgg
    11701 cgtgacgatt gcggtgacgc tgctcgcgtg gctcgctgtg cgccgtgtgc cggggctgcc
    11761 gcgcttcttg cggcggctcg cccttgcggt cttcctcggg actctggcgc aggcgccgat
    11821 cggcctgctc gcgatcgcga ccgaccttcg ctggccgatc gtgatggtcc atctgctcct
    11881 gtcgatcgct ttgctcgggg gcgcgaccgt tctcgtcctc gaagttcgct cggcgaccgt
    11941 gggtcgcatc gaaccgctcg ttccaccgga gttgcaacgg ctggggctcg cgttcgtctg
    12001 cgcggccttt gtgctcatcg tgagcggatc gcttgcaacc gcctccgggc cgcactccgg
    12061 cggcggcgcc gcggacaaga tcggccgggt gggacggttg gcgccgtttg tctatgccca
    12121 cgcgacgacc gtcgggatct tcctgctgac cttcatcttc agcctcggct acctcgccgc
    12181 tcgccgtgac cgttcgccgc gtctcttcaa gctcggcgca gcggttttcg tgttgctgct
    12241 cgtccagatg gggttgggag agctgcagtg gcggacgcat ctgccttggg gtctcgtgct
    12301 ggcgcacgtc ttcctggccg cgaccgtctg ggtcggcacc gtcgcccttg cgacggtgtt
    12361 cgtccggccg aacgtagact tctccgatcg ctgaagaact ccgcattgtc gaccgcccga
    12421 gcctgcggga gccggttctg atcgcagcat ttcgcggctg gaacgacggt ggccagggcg
    12481 cctcgcttgc aggcggttac ctcgccaaga tctgggaggc ggaccggttc gccgacatcg
    12541 agccggagaa cttcttcgac ttccaggcga cccgcccgca cgtctcgctc gtcgatggtc
    12601 agacgcggca catcgactgg cccgagaatg ccttctacca cgcgccgatc ccggatctcg
    12661 atcgcgacgc ggtgctgctg ctcgggatcg agccgaacct gcgctggcag accttcaccg
    12721 gcatgatcgt cgatctggca cgggagcttg gtgtccagat gatgatcacc ctcgggtcgt
    12781 tgctcgcgga cgtgccgcac acgagaccgt caccggtgac cgggggaggg acagatccgg
    12841 agctggtcga gcggctaggc ctgcagcgct cccgctacga aggaccgaca ggcatcgtgg
    12901 gcgtgctcca cgacgcctgc aaccacgcaa gcattccttc cgtcagcctc tgggccgccg
    12961 tccctcacta tgtctcgctc gcgccgagcc ctcgcgccgc gctcgcgctc tgtcagcggc
    13021 tcggcgacat tctcgggacc cagatcgaca cggccgagct tcaggaggcc tcggagcgct
    13081 acagcgagca ggtgactgag gcggtcgcat ccgacgagga gactcaggcc tacgtcgagg
    13141 agctcgagca gcgtgccgag ttgctggaag acgaggagga cttgccttca ggcgagtcgc
    13201 tcgccgccga gctcacgcgc ttcctgcgcg agcgcgagca cggcaacggt ggaccagagc
    13261 gcggcccgtc cgaacagcct tagtgccgct atccgaaggt aaatgcgtaa gccgcaaggc
    13321 cccgcgtaaa gctgagctcg agcgcgccgt cggcggcgct cttctgcgag acgagcgtgt
    13381 agagccggtc cccatcgacg cgtacggtgg gctgcgtctt cccgttcacc ttgaccgcga
    13441 cgaagccccg gccggtgagc acgaggtgca cggtgcgcgc ctggaagtgc acgcgcagac
    13501 gcgcgttgtc ctcggcgacg atgcgctcgc cctcgacctt ccacctgccc gcgtacgcgt
    13561 acgagtcttg cgtcagcgac gccgggaagc ggtacgtggc gacccggtcg gttcgcagcg
    13621 gcgagcccgt gtagttgccg atgcgtagat agccgaggta cgactccggc gtccgcagtt
    13681 cgagcggtgt ccggtccgtg tcacgtccgg cttgcgggag ccggcctgcc ttgccggccg
    13741 cgagcaagag gcggatgttg cgctcggttt gcgcgtagtc cccttcgccg aagtggacgt
    13801 cgcgcacccg tcccgcctgg tcgaccaggt agtcggcggg ccagtactgg ttcgagtacg
    13861 ccttccacgt cccgtagctg ttgtcgagtg cgacggggta gtcgacgccg aggcgcttga
    13921 cggctgcgcg gacgttgccg agatcgtgct cgaatgcgaa ctccggcgtg tgcacgccga
    13981 ggatgacgag gcccttcgag cggtagcgtg cgtcccacgc tttcaggtac gggagcgtgc
    14041 gcaggcaatt gatacacgag tacgtccaga agtcgacgag cacgaccttg ccgcggagct
    14101 tggggaggct gagtgggcgc gagttgatcc acgccgagac gtcgtggacg tcgggcgcgg
    14161 cgccgtagtc cttcagcggc accttgccga gttgcgaggc cagccgctgc ccgtgcgcga
    14221 cgaaggccgg cttggccttt ccgtcgaggc ggtcgagcct ctctttcgcc gttgctgtcc
    14281 cctcgatcgc gttctggact cgctgtgcat aacctggcac cttcatctgc agggtctccg
    14341 cccagccctg gtagatgaca accgcagctg cggccatcag cacgccgcca ccgatacgaa
    14401 cagcctgagc gtgccggcgg aaccgcgctg cgaggcctcg gctgccgtgc gcaatcgcga
    14461 gcagcggcaa tgcggcgccg acggcgtacg caatcgcgat cgcgacgatc caaccaccga
    14521 cgcgattcgt cgcgatgttt gccgtcagcg atgccagcac cggcccgccg cacggaacga
    14581 acacaaggcc gaggctggcg ccgaggaaga agcctccgcc gaggtcaccg gctcgccgcc
    14641 gtgtgagcag agcgaatggc cgctccacct gctcgcccac cttcggcaca atcagcgtca
    14701 gcgccagcag gagcagcaga gccgctccga tctggatctg gtgcttcgcg ctgatgccga
    14761 gcagtcccca caaccacgcg ccggcgagca ggaacgccgt gaaggtcgtg acaaggccgg
    14821 cggcgatcgc gtagggccgc cgcttcgtcc cgccgccggc gccaccggcg agcacgatcg
    14881 gcagcaccgg gaggatgcag ggcgtgaacg cggtgatgac gcccgccgcg aacgcgatcg
    14941 ggacgaggat gagcaccctg gttagtccag tttaggcggc cggcgtttca gcggacgccg
    15001 gctcgggctt gggggcgggc ttcttcctgg gccgcggttt cggcttttgc gcctccgcgg
    15061 ccgcgacctg ctgctcctgc ccctctggtg ccttggcctg ctgctgctgc tgctgctgct
    15121 gctgctgtcc ctgcgtttgc ggctgctgcg tgccgctctt cttgcgctta cgccgtttgc
    15181 gtctgcggga gcgaccggag ctcagcttcg gctgcggcag attctccgcc agcgcgcccg
    15241 cgacctcgtg aacggtgccg agccgctcac gcgcggcgtc gatgagttga gttgcgcgtg
    15301 gtgtgtatcc ctcggcggat gcgagcaacg ccgcaccgac cggcatgggc aactcgagcg
    15361 ccacgttgac cagcttcgct gcattctcct cgtgtcggtg accgcgggag ttcgccagtg
    15421 cgccgagcag ccgcttcgcc tccgggaact ccgcgctcgc gcgccggtcc tgcaccttcc
    15481 gcgaggcgcg cgcgaggcgc caggtccagc gggccaggat ctggtccggc gcgtccgcct
    15541 ttccttcggc aatcgctcgc agcaggacgc ggacaccgcc catccggtcc ttctcccagg
    15601 tcggcgcctg ggtcacgagc gtgacgagat cgtcgaagtc ggcccgctcg accgtcatcg
    15661 acacgcggtc ggagagggcg atcatgcgca ggcgccggtt cgggttctcc gcggcgcagg
    15721 tcgcgaggaa ctgctcgagg cacgccttcg atgcgccgcg tgccgtcagt ttctctacga
    15781 acgccgcacg ctcgtcaaag cggatctggc gtcccgcgat cgtcgcccag tagcgttgct
    15841 cgtcctcgtc gagatacttc ggatcgacgc gctgccactc acgggtgatg acggcgaggc
    15901 ggcggcgaac atcgggggtg accggaacga gccatggggc cggtgtgagc ttgcgacggg
    15961 cgcgccgctg gactctgcgg cgcggggccg gcgtgacgcg ggcaccctcg cgatagaagt
    16021 cggcatcgag gagcgagtgc agcgagatca cgcgctcgtt gtgcgtcgct gccttcggga
    16081 cgaagtcgac gacgatcccg gcctccttcc gcggatgcgt gcgcatgatc cggccgatcc
    16141 gctgttgata gacccgccgc gacgcggtcg gcgcgagatg catgcagatg gtcgcgcgcg
    16201 gagagttcca gccttcggcc agcagcatcg cgttgatgag gacgttgatc tcgccgcgct
    16261 cgtaggcggc cagcgtctcg gcaagccgca cgggcggtgt tcgcccggac acggcctcgg
    16321 ccttcagtcc cacggcgcga aactctttcg cgaggttgta cgcgtgatcg acgccggcgg
    16381 catagacgat gcccggcgtg ttgtcgaacc gctctcggta cagcgatgcg gcagcctggt
    16441 tcagcgcctg gtggtcgagt gtcttcgcca ggatctcctg gtcgaagtcg ccgccgacga
    16501 tcggcacctg gttgatcgcg gcgaccggcg ggacacgcag gtcgcgcaac ggcgcgatca
    16561 gcccgcgtcg cgctgcatcc tgcaacggca ggtcgtcgac cgatgcgggg aacacgtcgg
    16621 acacctgctt tgcgatcaac tgctcggtcg ctgtcatgcc gatgtagatc ggctccggga
    16681 atgcccggat cgccgcgctc gtcttctcgc cgagcgcggt gtgagcctcg tcgcagatca
    16741 cgagctggta tgcctcgcgg gagatcgtct cctggtgacg cgcgaaccag gcgtaggtct
    16801 ggatggtcag cgggttgccg ttctgtagcg attccttacc gcgttcgact gccggtgcga
    16861 acctgtcgcc gtatccctcg tcggtgagat cgcgctggaa ctgcgagacg agcaggcgac
    16921 ggtgcgtgag gatcaagact ccgagggagc gtgcggcttc cacgaatccc gctgccgcaa
    16981 tcgtcttgcc cgatgccgtg ggatgacgga accggtagcg gcgaaccgca ccggggtccg
    17041 gtccggtgta gtgctcgtcc tcctccacct cttcatcgag gacgtcctcc gcggactcct
    17101 cagccggctc gtcgtcgtct tcttccactt caacctcggc ctcgacgacc tcggcgacac
    17161 cgttgccgtt gccattgccg ttcggccccg cttccttctc gtgggacgcg atcagctcgg
    17221 tcagcatgcc ggcgagtgcg tcgatctggt ggcgccgcaa ctcggttccc gacgcgaggt
    17281 ggggcttctc ctccgccagc acccgctcga gtccgagcat gaagccgtac tcgaccttcc
    17341 acccggacga gggcgtgcgc tgacctccct cgatctcggc gagggcgtcg tctaatgcgc
    17401 gccggcggga agtgccgggc gccaggacct tggcgggatc ctcgccctcc cgaatgaagg
    17461 gctcgcccgc ccaggcttcg gctcggaggg cgtcggccac caggccgtcc gtcgcctccg
    17521 acgctgtggt cggctgctct ttgatttcct gcatggacgc agcgtctaaa agcgctgcac
    17581 tgtgtaagaa aaggcgaccc cgcgaatccc caaggcgggc gtcgtgcaac cagtatatcg
    17641 gttgggcggc taggtgccga gacccccgaa ggaggctagc ccgccagccc gccgaagacg
    17701 gcgaaggggt tctggggctg tacctcgtag accagctcgc ctcccggctc gacttcgccg
    17761 tggatcctgc gcgcgagctc gacggcctcc tcgcggctcc tggcgcctgc gatcacgtag
    17821 gtgaacgtcc gtgagacgtc gtagccctcg gaacggagct gctcggccag gtcgtgggcc
    17881 tcccggaggc tgtctgcctc gacccgcacc tcccagggcg cgtagccgcg ctggagcagc
    17941 tcctcctcga tgtccggctg ctcggggtcg tcgttcaagc gttcctcgtc ctggagccag
    18001 cgctcggtca cgaaccggga cggggtcagg ccctggtcct ccagctcgct ctcgatgatg
    18061 cgcgccgcct gctcggcctg catcccggat gcggcataga cgaacacggt gtcgccgtcc
    18121 tgggacacgg ccagccggtg ctcgcgcagg tcgtcggcga gctcctccgc gccgctctgt
    18181 cgaagcccga gccggcccag cagatcctgc gcgtcggcct cgtcgggcaa ttcgatccgg
    18241 atccgccaat cgtcatgcgc catgccggga gcctactttc gcgcggctcc agtcacacgt
    18301 tcgtaccggt gcctggcacc ggtacgacct ccgcgagccc gtacgccctg cggcgttgcc
    18361 ggcttctgcg acgaactcgt cacgcttgcg tacccgtgcc tggcaccggt acgtgtccag
    18421 ttaggcgcgg gccacggcgg tcagctccgc gaacttgtgg ccgaagagcc gctcggagat
    18481 gccgatctga tccaagtgct tcgtgatccc gccgaggaag aacggccatc cggccccgag
    18541 gaggaggcca gtgtcgacgt ccgcggcttc ggtgacgacg ccctcttcga ggatcagatg
    18601 aatctcgtcg gccacggctt cgaggacgtc ctcgacgatc tgctcgcggg ttcgcggcgc
    18661 gtcttcgagc acgaccggct cattgccgtc ggcgatcgcc gccagtgcgg gtgacaaggg
    18721 aaaacgctcc gggaacgcgt cgtgcatccg ttcgaggaca tggttcgcca cccgcgggcc
    18781 gaccatctgc agcaggacgg acggcgccat cggcattccg agtgacatga ccgcctcgtc
    18841 cacctcctcg accggggtcc cgtgctcgac ggcgtccatg atcacgcgcg tcattcgcgt
    18901 cagcacgcgg ttgacgacga agccgggcgc gtcgttgacg aggatcgggc gcttgcgcag
    18961 cttctcgccc gcgtcccaca ccgtcgcaag cgtcacctca tccgtctcgg cagcgcgcac
    19021 gacctcgagc agcggcatca gcgcgacggg attgaagaaa tgcatcccgg cgacccgttc
    19081 gggccggcgc aagtcggcgg ccatgtcagt gatcgacagc gccgaggtgt tggtcgccag
    19141 cacgcactcg tcccgcacaa cttgttccag ctccgcgaac acctgctgct tgatctcgag
    19201 ctcctcgaac accgcctcga gcacgaggtc gcagtcggcg aacccctcgt aatccgtgga
    19261 gcccgagacg agggacgcga ggaaccgtcc cttcccctcg tcgtagcgac ccttggccac
    19321 ctggctgtcg atcccggcgc ggatgtcggc gagcgccgca tcgacgatct cctgcttgac
    19381 gtcgcggatc acgatcggca cttccagccg gcgcaggagc agcgtcgcga tctgccgcgc
    19441 catcaggccg gcaccgacga tgccgactct gttcaccttc aacggcggca cggttggccg
    19501 agcagggtgc ctcttcgcgc gcagctcgac gagctggaag gcgtagagcg aggcctgtgc
    19561 ctgcgggccg ggcaggagtg cgccgatcgc ttcccgctcg cgggcgtagc cctcgtcgat
    19621 cgtccactcc tgggcgccgg cgatcaggtc gagcgcggcg tacggtgccg gcgctgcgcc
    19681 gtgcaccgtg tcgtccacgg cagtgcgggc tcggcggaag atcgtctctg catcggacca
    19741 gtcgggctcg ggccgctcga gcagctggtc ggcgagctcc cgtgcgaatg cgagcgactc
    19801 gtcgacgaac tcaaccggct cgagcagccg gtcggcgatg ccgagctcgg cgacctgagg
    19861 gcccgtcagc atccggttct gccgcagcgg attcgacacg atcaccttga tcgcggtttc
    19921 cggcccgaca aggcggggca agagctgcgt tccgccccac gcggggaaga ggccgaggaa
    19981 gacctccgga aacgcaaagt gccgaaccgc ggtggagatt gttcgcgcgc tgcagtgcaa
    20041 cgccagctcg acaccgccgc cgagacaggc accgttgatc gcggccacgg tcgggaacgg
    20101 caacgcacgg agtcgtccga acagctcgtg tccggcgcgc gagcctgcga tcgcgcgctc
    20161 gcgcgtgatg tcggggaact ccgtgatgtc cgcacccgcg gcgaagacga aaggcttgcc
    20221 cgtgatgacg gcggcgcgaa agtcgctctc ctcgagtcgg ccgagcagac gatcgagcga
    20281 ctcgagagcc tcctgcccga aaaaggtggg cttcgtccag tcctcgccgt tgtcgatcgt
    20341 cacgagcgcg acgtcgcccg ctcgcgtgag cttgaactca gtgaccggcg ccaaggttct
    20401 cccacagcaa agccgcaccc atgcccatgc cgacgcacaa cgccgtcaag ccgtaacgaa
    20461 cgtcgggtcg ctcgcgaaat ccgtacgcga gctgcgccat caggcgcacg ccggtcgcag
    20521 cgagcggatg tccgcacgcg attgcgccgc cgtacggatt gaggcgcgga tccttcggat
    20581 cgattcccat tccctcgcac caggtcagca cctgcacggc gaacggctcg ttcagctcga
    20641 acaggcccac gtcgtcgagc gccagtcctg cctgctcgag cacgcgcttc gtcgccggga
    20701 tcgggccgat gcccatcagc tccggctcga cgcccgcgaa cccgaacgca acgagccgca
    20761 tcttgggctc gagcccgagc tcggccgccg tttcctccgc ggcgagaaag caggcggttg
    20821 cgccgtccgt caaaccggcc gagttgcccg cggtaacgcg gccgcctgcg cgaaacggcg
    20881 tgcgcagcgt cgcgaggccc tcgagggtcg tatcagagcg aagaaactcg tcgctgtccg
    20941 cgagcttcca gccgtcgtcc gtgaacacgc tcatcggcac gacggtctcg cgcatgacac
    21001 cgttcgacca cgcagccgcg gcacgctgct gggattgcag ggcgaatgca tccgcgtcgt
    21061 gcttcgtgat ctgcgggaag cggtcgtgca ggttctctgc ggtcgcgccc atggtcaccg
    21121 cggagggatc gacgagccgc tccgaaacga agcgcggatt gaagtcgacg tcctcgccca
    21181 tcggatggtg tcccatgtgc tcgacgccgc cggcgagcac gatgtcaca
//
LOCUS       DASVEU010000112        20828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1629047,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000112 DASVEU010000000
VERSION     DASVEU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629047"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF225_04315"
     CDS             <1..884
                     /locus_tag="VF225_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144511.1"
                     /translation="DQRTEMTAREWPARVGAIIGNVEDAKAHAATAVALAQKLRERHE
                     LANACMYSGWLYALEGDWQTARELSDRALGLEPREPRNLATRALLELQVGALDDGERH
                     LEQLLDPRRVTAWSLLEKFAMAAFLPMAARVTGDDQRLDLARETAEAALAATKMPPFM
                     HLYGRIGLAFVAVQRTDRLLAAEQYAAFESQRGTAIMMAGIAVDRVLGLLAATLGQEE
                     KARRHLEDAIAFTRRANYRPEYAWAASDYADVLLEQGLPGDSERAHELHDEALATARS
                     LAMRPLAERIVAHSAAS"
     gene            complement(1126..2508)
                     /locus_tag="VF225_04320"
     CDS             complement(1126..2508)
                     /locus_tag="VF225_04320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7144512.1"
                     /translation="MTAVADEIQIDDAPFRGDVVRPGDPDYDVHRKIWNGSFDKHPAL
                     IIRCAGVSDVIAAVRFGRESGLPVAVRSGGHSFPGLSVADDALMLDLQPMKGVRVDPE
                     KRTARVQAGVLLGELDKETQAFGLAVPSGIVTHTGVAGLTLGGGIGWIQRKYGLSVDQ
                     LVSMDVVTADGEFVKASADENPDLFWGLRGGGGNFGIVTEFEFNCVPLGTQVLAGPIF
                     WPMEQSGEVLRFYRDWVADAPDDLMTIVVHRKAPPLPFVPAELHGKPVVMVIVCWAGD
                     VEEGETFIKPLREFGNPVADVCMPKPYLTHQAMLDPSFVPQRWYYFKSCDVAELSDEI
                     IDITVERALQIKSPLTSFPIWQMGGAVSRVGDDETPFGGRQAAFTYNIGCCTETADGF
                     DEEREWVRSFWSALEPWHQTVYVNFLEDEGEERVRTAYGAKKYDRLRNLKQKYDPDNF
                     FRINQNIPPA"
     gene            2644..2952
                     /locus_tag="VF225_04325"
     CDS             2644..2952
                     /locus_tag="VF225_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144513.1"
                     /translation="MGGIDFTFALAVGAFLLASWVDARVGDSRPEAPAKRIGLCLLAV
                     LALQGSVGGLYLVQAAGASQAGIMAAVLGLFLPALIFALLTGLWLLRMLADIVGVARR
                     "
     gene            2993..4084
                     /locus_tag="VF225_04330"
     CDS             2993..4084
                     /locus_tag="VF225_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144514.1"
                     /translation="MPEERDELEQAWQALTERERLLQQTMARRSGEIDARARRFDEIA
                     ADLDARRQLIEDAEEELVERERRLSLAEQEIQDRRAEIERAESEVERLRLQALELDRQ
                     ASELRVLASELEERGDWIATASAALEAQERRATVVAEQEAELEGRWTELKDEERVHDS
                     ASRQLDAKAAELEARAAELVAGEKRLALARSDEEPRLEAAKELQRDLEQREQRLVALE
                     ARHAEQEKQLGRRAAKLAVIETQLANKLRDVREADERNQMLEERARHLDARARDHERV
                     AKELDERGTWLTEAVARLEAREAAAAEEVERATAEVRRVERREQEFEDWSARLDDRDR
                     RLTEAYEALTERRRRVREDEIGLQPSEPG"
     gene            complement(4088..4582)
                     /locus_tag="VF225_04335"
     CDS             complement(4088..4582)
                     /locus_tag="VF225_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144515.1"
                     /translation="MIVRKAEASDAEGLTELGTAVGSEPEGWLVTTNGWRNVSDERRY
                     LRAIRRYPNAAVFVAEDEGLIVGRLSIARDQHPASRHVADIGLMVAASHRRRGIGTAL
                     LDAAVDWARRAGVHKLELHVFPWNEAAIRLYERFGFVQEGYRRKHYMRGDQYVDAILM
                     AYEL"
     gene            complement(4579..6063)
                     /locus_tag="VF225_04340"
     CDS             complement(4579..6063)
                     /locus_tag="VF225_04340"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7144516.1"
                     /translation="MRGAVAAGHPLTAQAGADVLAAGGNAVDAAVGAALVSWVAESPL
                     TGPGSGGFMLVHRASDRSTRVFDFFVSVPGLGLEQRDLAEMDRVDVDFSGGSTQVFHI
                     GAASCAVPGAPVGLEAAHRSFGKLPWRDLFQPAIELARDGVVLNNGQAYLHAILDLIL
                     RHTPESREVYERDGERLVAEDTLIQTDLAATLELFADEGGKALYEGELADKISEHLLA
                     HGGSITREDLGSYRVIRRRPVRASFCGEEYLSNPPPSAGGILIAYGLPLLEELGPGEP
                     GKAEAIDALARVMREQANARIAAGSIHGLARALETRAAVVSQGTTHISVVDGKGNAVA
                     LTASTGAGSGVVVPGTGIHINNMIGEFDLAKPPRPGTRLSSMMSPSLVLRAGRPHLVV
                     GSAGSLRLRGSVLQNVVNVVAHNLPVEDAIDRPRVHLEGEDLHCEGGHDPAELDKLEA
                     MGWNVTRWRRQNLYFGGASAVTFEDGKLAAAGDPRRGGAGVVVE"
     gene            6188..7303
                     /locus_tag="VF225_04345"
     CDS             6188..7303
                     /locus_tag="VF225_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7144517.1"
                     /translation="MPEERDLLERRLSREDLLRLAAVTGGAGLVAGRTGLARAASAHL
                     AKETGRLQVLDWAGYGNDGGQSMFAQYVKSHPQNKPRFTYMGNEADALAKIHAGLKPD
                     LFRPYVGWVKYFATSGLVQPWDPALISNFKHLNPYMVKAGQYQGKQYGIPDDWGFDAI
                     LYRSDKVKPKAKSWSLLFDERYKGKIAWYDDITMIQAAGLYLGFKNTWNQTDDQLKRS
                     QKLLISKKHLVRKIWSSETDLWQLFGSGDVWIAYAWPNDWVQMKKKGLKVVYMRPKEK
                     PIAWVGMFMLLQGTSRPQLAHAYVDAWSSTKSAKWLEDNYGYGHANTVARPSSSDLLR
                     ALQLTNLRAVTEPNAYMDRDIPRRALYAKTWEQVKAS"
     gene            7325..7480
                     /locus_tag="VF225_04350"
     CDS             7325..7480
                     /locus_tag="VF225_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144518.1"
                     /translation="MAVRRRRLRPDLTTPTLLGLPVAWLVVFFLVPIGIVAAYSFDVY
                     SLFPGKH"
     gene            7487..8206
                     /locus_tag="VF225_04355"
     CDS             7487..8206
                     /locus_tag="VF225_04355"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144519.1"
                     /translation="MAAWREFLHSSVYLKLFWKSVQMSLIVSAIIVVLAYPLAYYLAL
                     SGTKRKYVLLLVLIAPFLTSYLLRVLAWKVILGDQGVINSFLFWTGLRSADHPISQLL
                     YSRFAVMLVLGYVWLPFVALPIFVSLESLDRRLLEAASDLGASRLQAFRRVTLPLSLP
                     GIMAAFLFVFIPTLGEFVTPSLVGGPSGYMYGNQIVDLFGTGFPDWETGSVLAIFLFG
                     VVTVLTVVFARFLQPRQVTAG"
     gene            8206..9027
                     /locus_tag="VF225_04360"
     CDS             8206..9027
                     /locus_tag="VF225_04360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144520.1"
                     /translation="MDVALSRNGRIALRALFVLVVLFLYLPIAILLIFSFNNAEVPSF
                     PLSGFTLHWYHQFLTNGDLRGALQTSAIIAAISSVGAVVLGVLASVALTRRRVRGRAA
                     VSALLLSPLVIPYVVFGISLLLLFHTVGIPRSILTVVIGHIVISLPYTILVLVPRLEQ
                     IDVSLEEAAYDLGASRLRTFRSITLPLILPAVVSAFLIAFTTSFDEYAVASFVVGTRT
                     TFPIYLYSALRFPNQLPQVIAVAVVVISLSLIVVVAAEVGRRVAERRLGALDSAG"
     gene            complement(9024..10139)
                     /locus_tag="VF225_04365"
     CDS             complement(9024..10139)
                     /locus_tag="VF225_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144521.1"
                     /translation="MAADGSVAIALEGVSKGFGKTRAVQDVTVAIGEGEFFSLLGPSG
                     CGKTTTLRMIAGFEVPDEGRIVLQGKDVTSVSANHRKVNMVFQQYALFPHMSIYDNVA
                     FGLKVKRVPRSEHSERVHEMLRVVELEGLERRRTRQLSGGQQQRVALARALVNKPAAL
                     LLDEPLGALDVKLRKQMQFELKRIQAELGTTFVYVTHDQEEALAMSDRIAVMNGGRVE
                     QIGSPREIYEHPETTFVADFIGSLNALDLMIDELLGTYAVSRFGEDQRVVVPLTSGYR
                     TGDAVRVAVRPERVQIGSIDNPVRDGGSRLEGTIAEIVYLGMYVQFHVDTRAGRVVSH
                     RLADESLAPIEPGSRVALSWEPEQASVLADSAPLPEP"
     gene            10315..10737
                     /locus_tag="VF225_04370"
     CDS             10315..10737
                     /locus_tag="VF225_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7144522.1"
                     /translation="MLGILATLALSAAVTFPHGTAVIRTSGSTVRVSVEIAVTPAQQA
                     QGLSGRRALAPGSGMAFLWTSEIRGRFWMKDTSIPLSIAFWSKSGRILRILDMAPCRR
                     DPCKVYDPKVAFRGALEVNRGAFARWGVHPGARVTIRR"
     gene            complement(10857..12221)
                     /gene="ftsZ"
                     /locus_tag="VF225_04375"
     CDS             complement(10857..12221)
                     /gene="ftsZ"
                     /locus_tag="VF225_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7144523.1"
                     /translation="MARKRASMREGPLAELFKATEAAQRQQEQGAPAPAAEEPHESTV
                     EHVPTWEDEVETPAPPRPDPVPEPPPPEPVPLPEPPPPAAYIPEPPVTRYIEPMPEPA
                     PRLHHARAGQLGSYLAKIQVVGVGGAGLNAVNRMIDAGINQVEFVAVNTDVQQLQISD
                     AETKIHIGRELTQGLGSGSEATVGKAAAEESYDQIKHALRGADMVFVTAGEGGGTGTG
                     AAPIVAKIAKGLGALTVGIVTTPFKFEGTKRRGQAETGVDALRRECDTTIVIPNDRLL
                     EVLDKSTSMLDAFKIADDVLRQGVQGICDLITLPGLINLDFADVRTVMEGSGSALMGI
                     GFSSGTENRAREAAERALRSPLIDTELHGARGILLSIAGGDDLTLLEVNEAAEVIKRT
                     ATDDTQIIFGATIDDRLTGQVWVTVIATGLGGTGRSSSLTPSLVNALTAADDELEPPS
                     FLRN"
     gene            complement(12193..12963)
                     /locus_tag="VF225_04380"
     CDS             complement(12193..12963)
                     /locus_tag="VF225_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7144524.1"
                     /translation="MGRVISFANQKGGVAKTTTTLNLGVALKEKGHEVLLVDLDPQGN
                     LTMSQGWNPDEIERSMFDVLVHKLPIEEIVRQAEVDVAVSSIDLAGAELALSSMIGRE
                     RALEKALVSVKPKYDYILIDTPPSLGLLTINALVASNGVIVPVQCEYLSLRGLVQLEN
                     TLSMIRENLNPEVSIQGILPTMYDKRLLHSKEAVDILKENFGDLVFKTKIRKTIRYAE
                     APVKGQSVLGYEPSGDAAQLYRDLAKEVLNGAQAREHA"
     gene            complement(13120..14094)
                     /locus_tag="VF225_04385"
     CDS             complement(13120..14094)
                     /locus_tag="VF225_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144525.1"
                     /translation="MTLVFRGRAVVAALLAALALAGATGANAAIVTSHDDQGRTITFD
                     VRATAVDTEWYASVLRAAAHGNEISNVTITIVPEPQIPAVCGGQEAAACYSKTGTPTI
                     FVPAGKSRLLETILLHEYGHHLDTFWHVSGVPELNGTPTWWAARGMASLLASNQVAFD
                     YSLGWSHSIAEVFAEDYAYIHTHSRYGITWLSPPDAALQSAMFAELGTPTEPLPAAPE
                     VPLVISRRGTLSPRDRFSVPFGLLGPGRRVTMAATISKANRKGVRARAQLVCDGTVVA
                     TRTFRKGQAKRSLDIPDLGPATCDVRLLSSVSTSLAYTLRISLAVESV"
     gene            complement(14138..15106)
                     /locus_tag="VF225_04390"
     CDS             complement(14138..15106)
                     /locus_tag="VF225_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX7144526.1"
                     /translation="MGRARRLAGSRRFRIGINAVFVSGALAAAALTILHFVHSGWPLA
                     NADPLLVVAAGILFLFAYAFKAWGWQRLFPEGQRPEALTLAAAGGAASVGGVALPGRF
                     DEAIRVAVVRRGPGKKTAGLGAICLTLIIVGLLDSAALAPMASIAAAASGHGGVQAGF
                     ALVAAAGVAAAVLVLFLPKIAQMAFVSRWKVGRWLKDHTACKRSASEAWFLISISWAL
                     RGLAVFVLLNALSVGASFPLALAFLCASAASAALPIAPAGAATQAGAGAAILIIGGVP
                     KSEAFAFAVAAQGMVIIVGAALVGAAAIWMLFTRFRPSARTTALPA"
     gene            complement(15284..16213)
                     /locus_tag="VF225_04395"
     CDS             complement(15284..16213)
                     /locus_tag="VF225_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7144527.1"
                     /translation="MGITHWDDVEKFHRERGQMSATWTPLGGAAGSWKAGATRIELEP
                     GEQPTPPHIHGDAEEIFYVLGGSGWTLLDDDAYEIRAGDCIVYKSSHEAHTIRAGDDG
                     LDVIAFGAREYLPTGELPRGGTMWSITGFIELQEGHPWDREPHLFWPDPLPERPQTIV
                     NTQDVEPDIRGGRRRRDLARAAGSRWTGLKHIELDPGALSGPPHVHSAEEEILVVLDG
                     DGTLELTPSMPPATEPEQHPVRKGSVVARPPGTGIAHAFRAGDSGLTLIAWGTREPND
                     ICWYPRSKKIFWRGVGVIGRIEQLDYWEGEDLE"
     gene            complement(16223..16423)
                     /locus_tag="VF225_04400"
     CDS             complement(16223..16423)
                     /locus_tag="VF225_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144528.1"
                     /translation="MDEARAVLARLDRIEELEREGAPPGVLLEELRGLVQAAEAWAKL
                     EGDERARSAVERCDLSLAQPVA"
     gene            complement(16434..17675)
                     /locus_tag="VF225_04405"
     CDS             complement(16434..17675)
                     /locus_tag="VF225_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144529.1"
                     /translation="MIVRAPQLFDCLRAFCLGSFRALLQELDRGADLPFAFEEHTSYD
                     RPALYEYRPLVKPFIEARAHTLRQRPDAELAVDELRREPAAAIFARAHAGPRADDHEA
                     LFGSVLLPLLMSTAEMCGGFDWDDDAYVRAYAELEAALFGEGHQYGAVAPLIGITTGG
                     ATIELGGNIRIRLAATGELAKLWPEANGLLPTDFGREADRLCILELERPLEPGATEPP
                     DAPGEFADAITAIRLATAAPIAAGPVLFERLDWRPYGVRPVLPIAATQPAGEPTRLDS
                     FRAGLAADLLPRLARADDDPELGEALDRWELSLFQDGPFQAEQLRESLAALLGGSDGL
                     WAAAVRGAILLGDTPRARAELQDRLSGDPEADVVRRALVDTLLHGDRTELVASLDETI
                     LGLRPRPTRHTSATDTRAAAG"
     gene            complement(17684..18439)
                     /locus_tag="VF225_04410"
     CDS             complement(17684..18439)
                     /locus_tag="VF225_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7144530.1"
                     /translation="MPEGDALHRAARRLQVLVGQRLEVETPHPRAAVKQLAPRLDGRR
                     LENVEAIGKNLLLRFEGGLVLRSHLRMKGRWRVFRRDTPIFGQPWLVLRGDDYVGAMF
                     NGAVLELDRGVARRLGPDILAEQPDYDAMLARFRRADQQRQIGDALIDQGLVAGIGNL
                     WKAESLWLARVSPWRRLVQVSDEELRATLAEAHRLMNGRLEGVPGHARVYRRKGRPCP
                     RCGAEIRSWPQGDDARMAYWCPECQKGEEPRTA"
     gene            18451..18825
                     /locus_tag="VF225_04415"
     CDS             18451..18825
                     /locus_tag="VF225_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144531.1"
                     /translation="MSPRARAAAAGAVAALVWGLQEPLDQRVFGCDYSDVLFLGRGRR
                     SLGFAAHLANGALFGVAFDAVRRRVDVDGRRLALALALGEHVASWPFISLVDRRLVTS
                     PRAFAQATYRHALFGLVLGRLA"
     gene            complement(18822..>20828)
                     /locus_tag="VF225_04420"
     CDS             complement(18822..>20828)
                     /locus_tag="VF225_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144532.1"
                     /translation="QVARRYGSFPIILETYRECLQDVFDLPALKRLLQGLRTRELDLV
                     DVETASASPYSASLLFDYIATYMYEDDTPPAERRAQALSLDRDLLRELLGQEELRDLL
                     DAGAVEEVEAQLRGNPRNADQLHDQLRLRGDLREGEYDEGFAQTLLRERRALLVRFAG
                     EERLLAAEDAGRYRDALGVMPPGGLPEVFLEGGQESLAQLVSRYARGRGPFTTAQANE
                     RFGRDVEPLLQQLERQEKLLRGELRPGGTEREWCDPDVLRRLRRASLAALRKEVEPAE
                     QAAFGRFLPHWHGIDRRATLREALVPLQGLSLPVSLWESEVLPRRVPGFNPGQLDQLC
                     ASGEVVWVGAGLDRVAVFFREDAPALGRPAAAPRPEGDAHDRIRELLGRSAEFWYDLV
                     AATELEPEVALPGLWDLVWAGEVTNDAWTPLRAGRRYQTPRAQRGRPRRFSRQRSAGI
                     TATQGRWSLTDKLFPGRPDSRALAELVLERQGIITRDGVRGEGIPGGYGAVYGELRAL
                     ETLGVCRRGYFVEGLGGAQFALPGAVERLRELRPREDEEPEALVLPAADPAQPYGAAL
                     PWPKRADGRAARVAGAQVVLLGGEPALFVERGGRSLVPLRDPEEDWLRVALAALVEHV
                     KKGGSKKRLAVERFDSEPVGETEIMPLLLEAGFLAGPRRAVLRP"
BASE COUNT         3317 a         7045 c         7124 g         3342 t
ORIGIN      
        1 acgatcagcg gaccgagatg accgcacgcg aatggccggc acgcgtgggc gccatcatcg
       61 ggaatgtcga ggatgccaag gcccacgcgg cgaccgccgt ggccctcgcc cagaagctcc
      121 gtgagcgaca cgagctcgcc aatgcctgca tgtacagcgg ctggctttac gcactcgagg
      181 gcgactggca gacggcccga gagctgagtg accgcgcgct cggcttggag ccgcgcgaac
      241 cgcgaaacct cgccacgcgc gcgctgctcg agctccaagt cggagcgctc gacgatggtg
      301 agcggcacct agaacagctt ctggatccgc gtcgtgtgac agcctggtcg ctcctcgaga
      361 agtttgcgat ggctgctttt ttgccgatgg cggctcgggt caccggagac gaccagcgtt
      421 tggatctcgc tcgcgagacc gcggaggcag cgctcgcggc gaccaagatg ccaccgttca
      481 tgcatctcta tgggcgaatc ggcctcgcct ttgtcgctgt tcaacgcact gaccgtctgc
      541 tcgcagccga acagtacgcg gcgtttgagt ctcagcgagg cactgcgatc atgatggccg
      601 gaatcgcagt cgatcgcgta ctggggctgc tcgcggcgac gctcgggcaa gaggagaagg
      661 cccgccgcca cctcgaagat gcaatcgcgt tcacgcggcg cgccaactat cgacccgagt
      721 acgcctgggc ggcgtcggat tacgccgacg tcctactcga gcaggggctt cccggcgact
      781 cggaaagggc tcatgagctg cacgatgaag ccctcgcgac cgcccgtagc ctcgcaatgc
      841 gaccgctcgc agagcgcatc gtcgctcaca gcgccgcatc gtagtccggc gagcgcgagc
      901 acgagccacg tcctgcagag acgtggctga accgcctcaa tgagacatgc tgtttggcac
      961 cgaagtgtga ctttgtttcc acagccggct ggcttggaca tggtggggac aggccccgac
     1021 atgtctgtaa ccgtcggcta agagcgacgc gcggcggaag agagcgcaca gccgctcgag
     1081 caacagctca ggagatcggc ttgacgcagc gactcttgtc gaggtctaag ccggcgggat
     1141 gttctggttg atccggaaga agttgtccgg gtcgtacttc tgcttcaggt tccgcagccg
     1201 gtcgtatttc ttcgcgccgt aggcggtgcg aactcgctcc tcgccctcgt cttcgaggaa
     1261 gttgacgtac acggtctgat gccacggctc gagtgccgac cagaaagagc gcacccattc
     1321 ccgttcctcg tcgaagccgt ctgccgtttc ggtgcagcag ccgatgttgt acgtgaacgc
     1381 ggcctgcctg cctccgaacg gcgtttcgtc gtcgcccacc cggctgaccg cgccccccat
     1441 ctgccagatt gggaacgacg tcaggggaga cttgatctgt aacgcccgct ctacggtgat
     1501 gtcgatgatc tcgtcgctca gctccgcgac gtcacaactc ttgaagtagt accaacgctg
     1561 cggcacgaag ctcgggtcga gcatcgcctg gtgtgtcaga tacggcttcg gcatgcacac
     1621 gtccgcgacc gggtttccga actcgcgcag cggcttgatg aacgtctccc cctcctcgac
     1681 atcgccggcc cagcaaacga tcaccatcac gaccggcttg ccgtgcaact ccgccggcac
     1741 gaacggcaaa ggcggcgcct tcctgtgcac cacgatcgtc atcagatcgt ccggcgcgtc
     1801 cgcgacccag tcgcgataga agcgcaacac ctcgcccgac tgctccatcg gccagaagat
     1861 cggcccggcc aggacttggg tgccgagcgg aacgcagttg aactcgaact ccgtgacgat
     1921 cccgaagttg ccgccgccgc cgcggaggcc ccagaacaga tccgggttct cgtccgcact
     1981 cgccttgacg aactcgccgt cggcggtgac cacgtccatc gagacgagct ggtcgaccga
     2041 caggccatac ttgcgctgaa tccagccgat cccgccgccg agggtcagtc ccgcgacgcc
     2101 ggtgtgcgtg acgatgcccg acggcaccgc caagccgaac gcctgcgttt ccttgtcgag
     2161 ctcgccgagg agcacaccgg cctgcacgcg cgccgtgcgc ttctccgggt cgacgcgcac
     2221 gcccttcata ggctgcaggt cgagcatcaa cgcgtcgtcc gcgaccgaca ggccaggaaa
     2281 ggagtggccg ccgctccgca ccgcgaccgg caggccgctc tcccgcccga acctcaccgc
     2341 agcgattacg tccgacacgc ccgcgcagcg aatgatcagc gccggatgct tgtcgaacga
     2401 gccgttccag atcttgcggt ggacgtcgta gtccggatca cccgggcgaa cgacgtcccc
     2461 gcggaacgga gcgtcgtcga tctggatctc gtcggcgacc gccgtcatgg ccgctcagtt
     2521 tggcacgaac cggggaaacc gccaagcgtc tgccggaccg acgaaccgtt atccgtaggt
     2581 ctggggcccg caggacgttc ggggcgcgtg taggcggacg aattccccag gatgcgacta
     2641 gggatggggg gaatcgactt tactttcgcg ctcgcagtgg gtgcgttcct gctcgccagc
     2701 tgggtcgacg ccagggtcgg ggactcccgc ccggaggcgc ccgccaagcg gatcgggctc
     2761 tgtctcctcg cggtgctcgc cctgcagggc tcggtcggcg ggctctacct cgtccaggcg
     2821 gccggcgcgt cacaggccgg gatcatggct gccgtactcg gcctcttctt gccggctctg
     2881 atcttcgcgc tcctcaccgg cctgtggctg ctgcggatgc tcgccgacat cgtcggcgtc
     2941 gcacgccgct gatttacgcc cgcatctgat tcgcagggca tgatttgcag gaatgcccga
     3001 agagcgcgac gagctcgagc aagcctggca ggcgctcacc gaacgggagc gcctcctgca
     3061 gcagaccatg gcccgccgat cgggcgagat tgacgcacgg gcccgtcgct tcgacgagat
     3121 cgccgcggat ctcgacgctc gccggcagct gatcgaagac gccgaagagg aactcgttga
     3181 acgggagcgg cggttgtcgc tcgctgagca ggagatccag gaccgtcgcg ccgagatcga
     3241 gcgcgccgag agcgaggtcg agcggctacg cctgcaggcc ctcgagctcg acaggcaagc
     3301 aagcgagctg cgcgtgctcg cgtccgagtt ggaggagcgg ggcgactgga tcgcgactgc
     3361 gtcggccgcg ctcgaggcgc aggagcgtcg cgcgaccgtc gtcgccgagc aggaagccga
     3421 gctcgaaggt cggtggaccg agctcaagga cgaagagcgc gtacacgact ctgcatcgcg
     3481 gcaactcgac gcgaaggccg cggagctcga ggcgcgcgca gccgagctcg ttgcgggaga
     3541 aaagaggctc gcgctggcgc ggagcgatga ggagccgcgc ctcgaagccg cgaaagaact
     3601 ccagcgggac ctcgagcaac gagagcagcg gctggtcgcg ctcgaagcgc gccacgccga
     3661 gcaggagaag cagctcggtc ggcgcgccgc gaagctggcg gtcatcgaga cgcaacttgc
     3721 gaacaagctg cgcgacgttc gcgaggcgga cgagcggaat caaatgctag aggaacgcgc
     3781 gcggcacctt gacgcgcgcg cgcgggatca cgagcgcgtt gcgaaggagc tggacgagcg
     3841 gggaacctgg ctgacggagg cggtggcgcg tctcgaagcg cgcgaggctg ccgcggccga
     3901 agaggtcgaa cgcgcaacag cggaggtgcg gcgggtggag cggcgcgagc aagagttcga
     3961 ggactggtcg gcgcggctcg acgatcgcga ccgccggctg accgaggcct acgaagcact
     4021 caccgagcga cggcgtcgcg tgcgcgagga cgagatcggg ctccagccca gcgagcccgg
     4081 ctaggcgcta gagctcatag gccatcagga tcgcgtcgac gtactggtcg ccgcgcatgt
     4141 agtgcttgcg gcggtagccc tcctggacga agccgaagcg ctcgtagagc ctgatcgctg
     4201 cctcgttcca cggaaagacg tgcaactcga gcttgtgcac gcccgcgcgg cgcgcccagt
     4261 ccaccgcggc gtccagcagc gccgtgccga tgccgcgccg gcgatgcgac gcggcaacca
     4321 tcaggccgat gtccgcaacg tgacgacttg cgggatgctg gtcgcgtgcg atcgagaggc
     4381 ggccgacgat gaggccctcg tcctcggcca cgaacacggc tgcgttcggg tagcgccgga
     4441 tcgcgcgcag gtaacgccgc tcgtcggaga cattgcgcca gccgttcgtc gtcacgagcc
     4501 agccctccgg ctcggagccg accgcggtgc cgagctcggt cagcccttcc gcatcggacg
     4561 cctcggcctt gcggacgatc attcgaccac cacccccgcg ccgccgcgtc gggggtcgcc
     4621 cgccgcggcg agcttgccgt cctcgaaggt cacagcggac gcgccgccga agtagaggtt
     4681 ctggcggcgc cagcgggtca cgttccaacc catcgcctcc agcttgtcga gctcagccgg
     4741 atcgtgcccg ccctcgcagt gcaggtcctc gccctcgagg tggacgcgcg ggcggtcgat
     4801 cgcgtcctcg acggggaggt tgtgcgcgac gacgttgacg acgttctgca gcaccgagcc
     4861 gcgcaggcgg agcgagccgg cgctgccgac gacgaggtgc gggcgacctg ctctcagaac
     4921 gagcgacggg ctcatcatcg aggagaggcg cgttcctggc cgcggcggct tcgcgagatc
     4981 gaattccccg atcatgttgt tgatgtggat gccggtgccg ggaacgacga cgcccgagcc
     5041 tgctcctgtg gatgcggtca atgcgacggc gttgcccttg ccgtcgacga ccgagatgtg
     5101 agtcgtgccc tgcgagacga ccgcggcgcg tgtctcgagt gcgcgcgcga gcccgtggat
     5161 cgagccggcc gcgatgcgcg cgttcgcctg ttcgcgcatg acgcgtgcga gtgcgtcgat
     5221 cgcctcggcc ttgccgggct cgcccggccc gagctcttcg agcagcggga ggccgtaggc
     5281 gatcaggatc cctcccgccg acggcggtgg attcgagagg tactcctcgc cgcagaagct
     5341 cgcgcgcacc ggtcggcgtc ggatcacgcg gtagctcccg aggtcctcgc gcgtgatgct
     5401 gccgccgtgc gcaaggaggt gctcagagat cttgtcggcg agctcgcctt cgtagagcgc
     5461 cttgccgcct tcgtcggcga acagctcgag ggtcgcggcc aggtcggtct ggatcagcgt
     5521 gtcctccgcg acgaggcgct cgccgtcccg ctcgtacacc tcccgcgact ccggcgtgtg
     5581 gcggaggatc aggtcgagga tcgcgtgcag gtacgcctgg ccgttgttca gtacgacgcc
     5641 gtcgcgtgcg agctcgatcg ccggctggaa gaggtcgcgc caagggagct tgccgaagga
     5701 gcggtgcgct gcctccagcc cgaccggcgc accggggacc gcgcaggacg cggcaccgat
     5761 gtggaagacc tgagtcgagc cgccgctgaa gtcgacgtcg acgcggtcca tctcggcgag
     5821 gtcgcgctgc tccaggccga gccccgggac ggagacgaag aagtcgaaga cgcgtgtcga
     5881 gcggtcgctc gcgcggtgca cgagcatgaa gccgcccgag ccggggccgg tgagcgggct
     5941 ctcggcgacc caggagacga gcgcggcgcc gactgccgca tccaccgcgt ttcccccggc
     6001 ggcgagcacg tcggcgcccg cctgcgcggt cagcgggtgc ccggctgcga ctgcccccct
     6061 catgcgtgaa gggtagcccg gtcgggtgaa cacgttctcg ccacgaagtt ggcaccagct
     6121 tgacggcctc tggacggtcg agttcaatcg ccagacgggg gcctgcgagg gaaggagcac
     6181 gagcgcgatg cccgaggaga gagacctact ggagcggcgg ctcagccgtg aggacctact
     6241 taggctggca gctgtcaccg gcggcgcggg actggtcgcc ggacgaaccg ggctcgctcg
     6301 ggccgcgagc gcgcacctcg cgaaggagac cggacggctc caggtgctgg actgggcggg
     6361 ctacggaaac gacggcggcc aatccatgtt cgcgcagtac gtgaagagcc atccgcagaa
     6421 caagccgcgg ttcacctaca tgggcaacga ggcggacgct ctcgcgaaga tccacgccgg
     6481 tctcaagccg gatctcttcc gcccctacgt cggctgggtc aagtacttcg cgaccagcgg
     6541 cctcgtccag ccctgggatc cggcgctgat ctcgaacttc aagcacctga acccctacat
     6601 ggtcaaggcc ggccagtacc agggcaagca gtacggaatc ccggacgact ggggcttcga
     6661 cgcgatcctc taccgcagcg acaaggtgaa gccgaaggcg aagtcctgga gtctcctgtt
     6721 cgacgagcgc tacaagggca agatcgcctg gtacgacgac atcacgatga tccaggccgc
     6781 cggcctgtac ctcggattca agaacacgtg gaaccagacg gacgaccagc tcaagcggtc
     6841 acagaagctg ctcatctcca agaagcacct cgtccggaag atctggtcgt ccgagaccga
     6901 tctgtggcag ctcttcgggt ccggcgacgt ctggatcgcc tacgcgtggc cgaacgactg
     6961 ggtgcagatg aagaagaaag ggctgaaggt cgtctacatg cgcccgaagg agaagccgat
     7021 cgcctgggtc gggatgttca tgctgctcca ggggacatcg cgccctcagc tcgcccatgc
     7081 ctacgtcgat gcctggagct cgaccaagtc ggcgaaatgg ctggaggaca attacggcta
     7141 cgggcatgcg aacacggtcg cgcggccatc gtcgagcgac ctgctgcgcg cgctccagct
     7201 gacaaacctg cgggccgtta cggagccgaa cgcgtatatg gaccgcgaca tcccgcgccg
     7261 ggcgctctac gccaagacgt gggaacaggt gaaggcctcc tgaccggact gctctagaga
     7321 cgacatggca gtgcgccggc gacggctgcg gcccgacttg acgacgccca cgctgctggg
     7381 tctcccagtg gcgtggctcg tcgtcttctt cctcgtgccg atcggaatcg tggcggcgta
     7441 cagcttcgac gtctactcgc tcttcccagg caagcactga ttcacgttgg ccgcctggcg
     7501 cgagttccta cacagctcgg tgtacctcaa gcttttctgg aagtccgtgc agatgtcgct
     7561 gatcgtctcg gcgatcatcg tcgtcctcgc gtaccccctg gcgtactacc tggcgctctc
     7621 cggtaccaag cgcaagtacg tcctcctgct cgtgctgatt gctccgttcc tgacgagcta
     7681 cctgttgcgg gtgctcgcct ggaaggtgat cctgggtgac cagggcgtga tcaactcatt
     7741 cctgttctgg acgggactgc ggtcggcgga tcacccgatc tcccagctgc tctacagcag
     7801 gttcgccgtg atgctcgtcc tcggctacgt ctggctcccc tttgttgcac tgccgatctt
     7861 cgtctcgctc gagagcctcg accggcggct cctcgaggct gcgagcgacc tcggcgccag
     7921 ccgcctgcag gcgttccggc gggtgacgct gcccctgtcc ctgcccggga tcatggccgc
     7981 gttcctcttc gtcttcattc cgacactcgg cgagttcgtc acgccgtcgc tcgtcggggg
     8041 accgagcggc tacatgtacg gcaaccagat cgtcgatctc ttcggaaccg ggttccccga
     8101 ctgggagacc ggctcggtgc tcgcgatctt cctcttcggc gtcgtcaccg tgctgaccgt
     8161 cgtcttcgcg cgcttcctgc agccacggca ggtaacggca ggctgatgga cgtcgcgctc
     8221 tcgcggaacg gaaggatcgc gttgcgggcg ctgtttgtgc tcgtcgtcct cttcctctat
     8281 ctcccgatcg cgatcctgct gatcttttcg ttcaacaacg ccgaggtgcc ttcgttcccg
     8341 ctgagcggat tcacgctcca ctggtaccac cagttcctca ccaacggcga cctgagggga
     8401 gcgctgcaga cgagcgcgat catcgcggcg atctccagcg ttggcgccgt cgtgctcggc
     8461 gtgctcgcct ctgtcgcgct gacccgtcgt cgcgtcaggg gaagggcggc cgtctcggca
     8521 ctgcttctga gccccctcgt gatcccttac gtggtcttcg gaatctcgtt gctcctcctc
     8581 ttccatacgg tcgggatccc gcgctcgatt ctgacggtcg tcatcggaca catcgtgatc
     8641 agcctcccgt acacgatcct tgttctcgtg ccgaggctcg agcagatcga cgtctcgctc
     8701 gaggaggctg cgtacgacct cggcgcgagc cgcctgcgga cgtttcgctc gatcacgttg
     8761 ccgttgatcc tgccggcggt cgtgtctgca tttctgatcg cattcacgac gtcgttcgac
     8821 gagtacgcgg ttgcctcgtt cgtcgtcggg acgcggacga cgtttccgat ctacctgtac
     8881 tcggcgcttc gcttcccgaa ccagctaccg caggtcattg ctgtggcggt ggtcgtgatc
     8941 agcctgtccc tgatcgtcgt cgtggccgcc gaagttgggc gacgggttgc cgagcggcgg
     9001 ctcggcgccc tcgacagcgc aggctaaggc tccggcagcg gggccgaatc ggccagcacg
     9061 gaggcctgct ccggctccca ggacagggcg acgcgcgagc cgggctcgat cggcgcaagc
     9121 gactcgtcgg cgaggcggtg gctgacgacg cggccggcgc gggtgtcgac gtggaactgg
     9181 acgtacatgc ccagatacac gatctcggcg atcgtcccct cgagccgcga ccctccgtcg
     9241 cggacgggat tgtcaatcga cccgatctgg acgcgttcgg gccgcaccgc gacgcggacg
     9301 gcatcaccag tccggtagcc agaagtgagc gggactacga cccgctggtc ctcgccgaac
     9361 cgactcactg cgtatgtgcc gaggagctcg tcgatcatca aatcgagcgc gttgagcgaa
     9421 ccgatgaagt ccgcgacgaa agttgtctcg ggatgctcgt agatctcgcg ggggctgcca
     9481 atctgctcca ctcggccgcc gttcatgacg gcgatccgat cggacatcgc gagcgcctct
     9541 tcctgatcgt gcgtaacgta gacgaaggtc gttcccagct cggcctggat ccgcttgagc
     9601 tcgaattgca tctgcttgcg gagtttcacg tcgagcgctc cgagcggctc gtcgagcagc
     9661 agtgccgcgg gcttgttgac gagagccctc gccagcgcga cacgctgctg ctgacctccc
     9721 gagagctgac gggtgcgacg gcgctccaga ccctcgagct cgacgacgcg cagcatctcg
     9781 tgcacgcgct cggagtgctc gcttcgcggg acgcgcttca ccttgagccc gaaggcgacg
     9841 ttgtcgtaaa tcgacatatg ggggaagagc gcgtactgct gaaagaccat gttcactttg
     9901 cggtgattcg ccgaaaccga ggtcacgtct ttgccctgca agacgatgcg gccttcgtcc
     9961 ggcacctcga agccggcgat catgcggagg gtcgtcgtct tgccgcaccc gctcgggccg
    10021 agcagcgaga agaactcgcc ctcgccgatc gcgaccgtga cgtcttgcac ggctcgggtt
    10081 ttgccgaagc ctttgctgac gccctctagc gcgatcgcga cgctgccgtc cgccgccatc
    10141 gtgcgagtct aatccggtgt gaggggagag cccgcgccgc cgctggcgcg ggctcttgcg
    10201 aggggccgca gttctgggac tgacctgtgc caagtagatg cacgcggccg cgtccgctta
    10261 cggcgtgcct gcgaagttca ctcgaacgag ttacggctcc ctcacaatgg gtcggtgctc
    10321 ggcatcctcg cgacgcttgc gctctctgcc gcggttacgt tcccccacgg gacggccgtg
    10381 atccgcacgt ccggctcgac ggtgcgcgtt tcagtcgaga tcgccgtgac accggcacag
    10441 caagcgcagg gcctgagcgg ccggcgcgcg ctcgcaccgg gctcggggat ggcgtttctc
    10501 tggacaagcg agatccgggg ccggttctgg atgaaggaca cgtcgatccc gctctcgatc
    10561 gcgttctgga gcaagagcgg ccggatcctg cggatcctcg acatggcgcc gtgccggcgc
    10621 gacccctgca aggtctacga cccgaaggtc gcgtttcgcg gcgcgctcga ggtgaatcgc
    10681 ggcgcgttcg cgcgctgggg cgtccacccc ggcgcccgcg tcaccatccg gcgctaacga
    10741 gacgtaccgg tgccaggcac gggtacgaaa ctgtgacgcc ctgcctgaaa ggcgctgctc
    10801 ctcgcgggaa attacccgtg ccaggcccgg gtacgtcccc gtaacgagag cgtgtcctag
    10861 ttccgcagga aggagggcgg ctcgagctcg tcgtcggctg cggtcagcgc gttgaccagg
    10921 ctcggggtga gcgagctgct gcggcccgtg ccgcctaggc ccgttgcgat caccgtcacc
    10981 cacacctggc ccgtgaggcg gtcgtcgatc gtcgcgccga agatgatctg cgtgtcgtcg
    11041 gtcgcggtcc gcttgatgac ctccgccgcc tcgttcacct cgagcagggt caggtcgtcg
    11101 ccgcccgcga tcgagaggag gatgccgcgc gcaccatgga gctcggtgtc gatcaggggc
    11161 gagcgcagcg cacgctcggc cgcctcacgc gcgcggttct ccgtgcccga cgagaacccg
    11221 attcccatca gcgcgctgcc ggagccttcc atgaccgttc gcacatccgc gaagtcgagg
    11281 ttgatcaggc cggggagcgt gatcaggtcg cagatgccct gtacgccctg gcggagcacg
    11341 tcgtcggcga tcttgaacgc gtccagcatc gaggtcgact tgtcgagcac ctcgagcagc
    11401 cggtcgttcg gaatcacgat cgtcgtgtcg cattcgcgcc gcaacgcatc gactccggtc
    11461 tcggcctgcc ctcgccgctt cgtgccctcg aacttgaacg gcgtcgtcac gatgccgacg
    11521 gtgagcgcgc cgaggccctt cgcgatcttc gcgacgatcg gtgcggcgcc ggtaccggta
    11581 ccgccacctt cgcccgcagt gacgaacacc atgtccgctc ctcggagcgc gtgcttgatc
    11641 tggtcgtagc tctcctcggc cgcggccttc ccgaccgtcg cctccgaccc cgagccgaga
    11701 ccctgcgtca actcgcggcc gatgtggatc ttcgtctccg cgtccgagat ctgcagctgc
    11761 tgcacgtcgg tgttgaccgc gacgaactcg acctggttga tgccggcgtc gatcatccgg
    11821 ttcaccgcgt tcagaccggc gccgccgacg ccgacgacct ggatcttcgc gaggtacgaa
    11881 cccagctgtc ccgcgcgcgc gtgatgcaag cgcggcgccg gctcgggcat cggctcgatg
    11941 tagcgcgtta ccggaggctc gggaatgtac gccgccggcg gaggtggctc gggtagcggt
    12001 acgggctcgg gcggcggcgg ctccggcacg ggatcgggac gcggcggcgc cggcgtctcg
    12061 acctcgtcct cccacgtcgg tacgtgctcg accgtcgact cgtgcggctc ctcggcggcc
    12121 ggggcgggtg ctccctgttc ctgctggcgc tgcgccgcct cggttgcctt gaagagctct
    12181 gcgagagggc cctcacgcat gctcgcgcgc ttgcgcgcca ttgagcacct ccttagcgag
    12241 gtcgcggtac agctgcgcgg catcgccgct cggctcgtag cccagtaccg actggccctt
    12301 cacgggagcc tctgcgtacc ggatcgtctt gcgaatcttc gtcttgaaga cgagatcacc
    12361 gaagttctcc ttgaggatgt cgaccgcttc cttcgagtgc agcaggcgct tgtcatacat
    12421 cgtcgggagg attccctgga tcgagacctc cgggttcagg ttctcgcgga tcatggacag
    12481 agtgttctcc agttgcacca gcccgcgcaa ggagaggtac tcgcactgca ccggaacaat
    12541 cacgccgttc gacgcgacga gcgcgttgat cgtcagcagc ccgagggacg gcggcgtgtc
    12601 gatgaggatg tagtcgtact tcggcttgac gctcacgagc gccttctcga gcgcgcgctc
    12661 acgaccgatc atcgacgaga gcgcgagctc ggcgccggca aggtcgatcg atgacacggc
    12721 gacatcgacc tcggcctgcc ggacgatctc ctcgatcggc agcttgtgga cgagcacgtc
    12781 gaacatcgac cgctcgatct cgtccggatt ccagccctgg ctcatcgtca ggttgccctg
    12841 cggatccagg tcgacgagca ggacctcgtg acccttctcc ttgagggcga ctccgaggtt
    12901 cagcgtggtc gtcgttttcg ctacgccacc cttctggttg gcgaacgaga tgaccctgcc
    12961 caaaggtgat cccttcctag tggacgaagc gcgtgtgttg ggacgcggaa gtaaacgtga
    13021 gagccccacg gggcgctctt tcgggccagg atagcgagtt cctgccgata taccgcatga
    13081 tcacgggcct ctcggacgcc gccctcgcgg ccggggaggc taaacgctct cgacggccag
    13141 gctgatgcgc agggtgtagg ccagcgacgt cgagacgctg ctgagcaggc ggacgtcgca
    13201 ggtggccggg cccaggtccg ggatgtcgag cgatcgcttg gcctgaccct tgcggaacgt
    13261 gcgtgtcgcg accaccgttc cgtcgcagac gagctgagcg cgagcccgga ctcccttgcg
    13321 gttcgccttc gagatcgtgg cggccatggt cacccggcga cccgggccga gaagaccgaa
    13381 gggaacgctg aagcggtcac gcggggaaag ggttccgcgc ctgctgatga ccagcgggac
    13441 ttcgggggca gcgggaagag gttcggtggg agtgccgagc tcggcgaaca tggccgactg
    13501 aagggcggcg tccggcgggc tcagccacgt gatgccgtac cggctgtgcg tgtggatgta
    13561 ggcgtagtcc tcggcgaaga cctcggcgat gctgtgactc cagccgagcg agtagtcgaa
    13621 cgcgacctgg ttgctcgcga gcagcgacgc catcccccgc gcagcccacc acgtcggcgt
    13681 cccgttcagc tccggaacgc cggagacgtg ccagaacgta tcgaggtgat gaccgtactc
    13741 gtgcagcaag atcgtctcga gcagcctgct cttcccggcg ggaacgaaaa tcgtgggcgt
    13801 gccggtcttg ctgtagcacg ccgctgcctc ctggccgccg cagaccgcgg ggatctgcgg
    13861 ctcgggaacg atggtgatcg tcacattgct gatctcgttg ccgtgcgcgg ccgcgcgcag
    13921 aacgctcgcg taccactcgg tgtcgacggc ggttgctcgc acgtcgaagg tgatcgtccg
    13981 gccctggtcg tcgtgcgacg tgacgatcgc agcgttcgcg ccggtggcac ccgcgagggc
    14041 cagggctgcg aggagcgcag cgacgacggc gcgaccgcgg aacacgagcg tcatcgaatc
    14101 acgcccgcct aagagccgcg taaggagtcg gtaaaagcta ggcggggagc gcggtcgtcc
    14161 gggcagacgg gcggaatcgt gtgaagagca tccagatcgc tgcggcgccg acgagcgcgg
    14221 ccccgacgat gatgaccatt ccctgcgccg ccacggcgaa cgcgaaggcc tcggacttgg
    14281 gcacgccgcc gatgatcagg atcgcagcgc cggcgccggc ctgcgtcgcc gcgcccgccg
    14341 gggcgatcgg caacgccgcg gacgcggcgg aagcgcagag gaaggcaagc gcgagcggga
    14401 acgaagctcc caccgagaga gcgttcagca ggacgaagac agcaagccca cggagcgccc
    14461 acgaaatgga gatcaggaac caggcctccg acgcggagcg cttgcaggcg gtgtgatcct
    14521 tcagccagcg accgaccttc cagcggctga cgaaagccat ctgcgcgatc ttcgggagga
    14581 agaggacgag caccgctgct gcaactcctg ccgcagcaac gagcgcgaag ccggcctgga
    14641 caccgccatg gcccgaagcg gcggcggcga tcgacgccat cggcgccagc gctgcgctgt
    14701 cgagcaagcc gacgatgatc agggtcaggc aaatggcgcc aagacctgcc gtcttcttgc
    14761 cggggccgcg acgaacgacg gcgacgcgga tcgcctcgtc gaagcgtccc gggagggcca
    14821 cgccaccgac agacgcggcg ccgcctgcag ccgccagcgt cagggcctca gggcgctggc
    14881 cttcaggaaa gaggcgctgc cagccccacg ccttgaaggc gtaggcgaag agaaagagga
    14941 tcccggcggc gaccacgagg agcgggtcgg cgttggcgag cggccagccc gagtgcacga
    15001 aatggagaat cgtcagggcg gcagcggcaa gcgccccgga gacgaacacg gcgttgatgc
    15061 cgatacggaa tctccgagag cctgcgagac ggcgcgctct ccccatacga ggagatatcg
    15121 ggaactccgc taggaggcta atgacgcgaa tgagggacta ctttcgtggg tgaggccggg
    15181 agcgagacgc gagagtgaac tgagttctgc gagaggggcg tcactcctcg ggctcgtcct
    15241 catcgtagcc caatagctcc agacttgtga tcccggcgaa cgatcactcg aggtcttcgc
    15301 cttcccagta gtcgagttgc tcgatgcgcc cgatcacgcc gacgccgcgc cagaagatct
    15361 tcttcgaacg cgggtaccag caaatgtcat tcggctcgcg cgttccccag gcgatcagcg
    15421 tcagcccgga gtcgccggcg cggaaagcat gcgcgattcc ggtgccgggt gggcgggcga
    15481 cgaccgagcc cttgcgcacc ggatgctgtt cgggctccgt ggccggtggc atcgaaggcg
    15541 tgagctcgag cgtgccgtcg ccgtcgagca cgaccaggat ctcctcctcg gcggaatgca
    15601 cgtgcggcgg accggacagc gcgcccgggt ccagctcgat gtgcttgagg cccgtccagc
    15661 gcgagccggc ggcgcgcgcc agatcccgac gtcgtcgccc accgcggatg tcgggctcga
    15721 cgtcttgcgt gttgacgatc gtctgcgggc gctcgggcaa cggatccggc cagaacagat
    15781 gcggttcgcg gtcccaagga tgtccttcct gcagctcgat gaagccggtg atcgaccaca
    15841 tcgtgccgcc gcgcggcagc tcgccggtcg gcagatactc gcgcgcaccg aaagcgatca
    15901 cgtcgaggcc gtcgtcgccc gcgcgaatcg tgtgcgcctc gtgcgagctc ttgtagacga
    15961 tgcaatcgcc cgcgcgaatc tcgtatgcgt cgtcatcgag cagcgtccat cccgagccgc
    16021 cgagcacgta gaagatctcc tcggcgtcac cgtggatgtg aggcggcgtc ggctgctcgc
    16081 ccggctccag ctcgatccgg gtcgcgcctg ccttccagct gccggccgca ccgccgagcg
    16141 gtgtccacgt cgcggacatc tggccgcgtt cccggtgaaa cttctcgacg tcgtcccagt
    16201 gcgtgatgcc cacgccacga ctttatgcga cgggttgcgc gagagagaga tcgcagcgtt
    16261 ctaccgcgct gcgtgcacgt tcgtctcctt ccagtttcgc ccaggcctcg gccgcctgga
    16321 cgagcccgcg cagctcctcg agcaggacgc cggggggtgc gccctcacgt tcgagctctt
    16381 cgatccggtc gagccgggcc aacactgcgc gggcctcgtc catgcggacg aagctagcca
    16441 gctgcagcgc gtgtgtctgt ggcggaagtg tgacgagttg ggcgcggccg caaaccgagg
    16501 atcgtttcgt cgagcgacgc gacgagctcc gttcggtcgc cgtgtaagag cgtgtcgacg
    16561 agcgcacggc ggaccacgtc cgcctcggga tcgcccgaca ggcgatcctg caattcggcg
    16621 cgtgcccgcg gcgtgtcgcc gaggagaatg gcgccgcgaa cggcggcggc ccacagtccg
    16681 tcgctgccgc cgagcagcgc ggcaagcgac tcgcgcagct gttccgcctg gaacgggccg
    16741 tcctggaaga gcgagagctc ccagcggtcg agcgcctcgc cgagctccgg gtcgtcgtcc
    16801 gcgcgcgcga ggcgcggcaa gagatcggcg gcgaggcccg cacggaagga gtcgaggcgc
    16861 gtcggttctc cggcgggctg cgtcgcggca atcggcaaga cgggacgaac tccatagggc
    16921 cgccaatcga gccgctcgaa caagacaggg ccggcggcga tgggcgccgc ggtggcgaga
    16981 cggatcgcgg tgatcgcgtc cgcgaactca ccgggcgcgt cgggcggctc ggtcgcgccg
    17041 ggctcgagtg ggcgctcgag ctcgaggatg cagagacggt cggcctcccg gccgaagtcc
    17101 gttgggagca agccgttggc ctccggccag agcttggcaa gctcgcctgt tgcagcgaga
    17161 cggatgcgga tgttgccgcc gagctcgatc gtggcgccgc cggtcgtgat cccgatcagc
    17221 ggtgcgacgg cgccgtactg gtggccctcg ccgaagagcg cggcctcgag ctccgcatac
    17281 gcgcgcacgt acgcatcgtc gtcccagtcg aagccgccgc acatctcggc ggtgctcatc
    17341 agcagcggga ggagcacgct gccgaagagt gcttcgtgat cgtcggcccg cgggcctgcg
    17401 tgcgcgcgcg cgaagatcgc cgctgccggc tcgcggcgaa gctcgtccac cgcaagctcg
    17461 gcgtcgggcc gctgtcgaag cgtgtgtgcg cgcgcttcga tgaacggttt cacgagcggc
    17521 cggtactcgt agagcgcggg tcggtcgtaa gaggtgtgct cctcgaaggc gaacgggagg
    17581 tcggcgccac ggtccagctc ctggaggagg gcgcggaacg agccgaggca aaaagcccgg
    17641 agacagtcga agagctgcgg agcgcgcacg atcacaactg cgcttacgcc gtcctcggtt
    17701 cctctccttt ctggcactcg ggacaccagt aggccatgcg cgcgtcgtcg ccctgcggcc
    17761 acgagcggat ttcggcgccg cagcggggac aaggccgacc cttgcggcga tacacccggg
    17821 cgtgaccggg gaccccttcg agccggccgt tcatgaggcg atgcgcctcc gcgagcgtcg
    17881 cgcgcagctc ctcgtccgag acctgcacga gccgtcgcca cggggagaca cgggccagcc
    17941 agagcgactc cgccttccag aggttgccga ttccggccac gagtccctgg tcgatcagag
    18001 cgtctccgat ctggcgttgc tggtctgcgc gcctgaatcg cgcgagcatg gcgtcatagt
    18061 cgggctgttc cgcgaggatg tcgggaccga ggcggcgcgc gacgccgcgg tcgagctcca
    18121 gcaccgcgcc gttgaacatc gcgccgacgt agtcgtcccc gcggagcacg agccatggtt
    18181 ggccgaagat cggcgtgtcg cggcggaaga cccgccagcg tcccttcatg cgcaggtggc
    18241 tgcggagcac gagcccgccc tcgaagcgca gcaggaggtt cttgccgatc gcctcgacgt
    18301 tctccaggcg gcggccgtcc agccgaggcg cgagctgttt caccgcggcg cgcgggtgcg
    18361 gcgtctcgac ctcgagcctt tgtcccacga ggacctggag gcgtcgcgcg gcacggtgaa
    18421 gagcgtcccc ttcgggcatg ctgcaagggt atgtcgcctc gtgcgcgtgc cgcagccgcc
    18481 ggtgccgttg cggcgctcgt ctggggattg caggagccgc tcgaccagcg ggtgttcggc
    18541 tgcgactact cggacgtgtt gtttctcggc cgcgggcggc gctcgctcgg cttcgcggcg
    18601 cacctggcga acggcgcgct cttcggtgtc gcattcgacg cagtgcggcg gcgcgtcgac
    18661 gtcgacgggc gacgactcgc gctcgcgctg gcgctcggag agcacgttgc gtcgtggccg
    18721 ttcatcagtc tcgtcgatcg ccgcctcgtc acaagcccgc gcgcgttcgc gcaggcgacg
    18781 tatcggcacg cgctcttcgg gttggtgctc ggccggctcg cctagggtcg gaggaccgcg
    18841 cggcgggggc cggcgaggaa gccggcctct aagaggagcg gcatgatctc cgtctcgccg
    18901 acaggctcgc tgtcgaagcg ctccaccgcc agtcgcttct tcgagccgcc cttcttgacg
    18961 tgctcgacca gcgcggccag tgcgacgcgc agccagtcct cctccggatc tctgagcggc
    19021 accagcgacc gtcctccacg ctcgacgaac agcgccggct cgccgccgag caggacgacc
    19081 tgcgcgcctg cgacgcgtgc tgcacggccg tccgcgcgct tcggccacgg cagcgccgcg
    19141 ccgtagggct gcgccggatc cgcggccggc aacacgagag cctcgggctc ctcgtcctcg
    19201 cgaggacgca gctcgcgcaa tcgttccacc gcaccgggca gcgcgaactg tgcaccaccg
    19261 aggccttcga cgaagtagcc gcgccggcag accccgagcg tctcgagtgc gcgcagctcg
    19321 ccgtacaccg cgccgtaccc gccgggaatc ccctcgccgc ggacgccgtc gcgcgtgatg
    19381 atgccctgcc gctcgagaac caactctgca agtgcgcgcg aatccggccg tcccgggaag
    19441 agcttgtcgg tcagcgacca ccgtccctgc gtcgcggtga ttcccgccga gcgctggcgt
    19501 gagaagcggc gcgggcgtcc gcgctgggcg cgcggcgtct ggtagcggcg accggcgcgc
    19561 aatggcgtcc aggcgtcgtt cgtcacctcg ccggcccaga cgagatccca cagaccgggc
    19621 agcgcgacct cgggctcgag ctccgtcgcc gcgacgaggt cgtaccagaa ctccgcactg
    19681 cgtccgagca gctcgcggat cctgtcgtgc gcgtcgccct cgggccgagg cgcggcggcc
    19741 ggacggccca gtgccggtgc gtcctcgcgg aagaagacgg ccacgcggtc gaggcccgcc
    19801 ccgacccaga cgacctcgcc ggatgcgcac agctgatcca gctgaccggg attgaagccg
    19861 gggacgcggc gcggcagcac ctcggactcc cacagcgata ccggcaacga caatccctgc
    19921 agcggcacga gtgcttcgcg caacgtcgca cgccggtcga tcccgtgcca gtgcggcagg
    19981 aagcggccga acgccgcctg ctcggcgggc tcgacctcct tgcgcaacgc cgcaagcgac
    20041 gcgcggcgca ggcgccgcag gacgtcaggg tcacaccact cccgctcggt tccaccgggc
    20101 cgcagctcgc cgcgcaggag cttttcctgc cgctcgagct gctggagcaa cggctcgacg
    20161 tcgcggccga accgctcgtt cgcctgcgcg gtcgtgaatg gcccgcgccc gcgcgcataa
    20221 cgcgacacga gttgcgcgag agactcctgg ccgccctcga ggaagacttc gggcagaccg
    20281 cccggcggca tgacgccgag ggcatcgcgg tagcggcccg catcctcagc ggcaagcagc
    20341 cgctcttcgc cggcgaagcg cacgagcagc gcgcggcgct cgcgtagaag cgtttgcgca
    20401 aagccctcgt cgtattcacc ttcgcgcaga tctccgcgca gacggagctg gtcgtgaagc
    20461 tggtcggcat tgcgcgggtt accacggagc tgcgcctcga cctcctcgac cgctcctgcg
    20521 tcgagcaggt cgcgtaactc ctcctggccg agcagttcgc gcaggaggtc gcggtcgagc
    20581 gacagtgcct gtgcccgccg ctcggcggga ggcgtgtcgt cctcgtacat gtacgtcgcg
    20641 atgtagtcga agaggagcga cgcggaatac ggcgacgccg acgccgtctc gacatccacg
    20701 agatcgagct cgcgcgtgcg cagtccttgc agcagccgct tcagcgcagg caggtcgaag
    20761 acgtcctgca gacactcgcg gtaggtctcg agaatgatcg ggaacgagcc gtagcgcctc
    20821 gcgacttg
//
LOCUS       DASVEU010000113         6187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_851059,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000113 DASVEU010000000
VERSION     DASVEU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851059"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..566
                     /locus_tag="VF225_04425"
     CDS             177..566
                     /locus_tag="VF225_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144533.1"
                     /translation="MEAAANEPDRIRVLIADDHRLFGQALEAILATDDRLEVVGHAGD
                     GSEAVQLALSVKPEVILMDISMPVMDGFQATKQIRKQRPHARILMLTGSNSRVDVDRA
                     REAGAAGYVTKNRIAAELIDAILEVAG"
     gene            591..812
                     /gene="secG"
                     /locus_tag="VF225_04430"
     CDS             591..812
                     /gene="secG"
                     /locus_tag="VF225_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7144534.1"
                     /translation="MVQALAVIQVLLSMGLVALILMHSGRDAGLGGMGFTPASQGGTH
                     IVERNLTRLTVVIAVLFVTNSILLFHALK"
     gene            911..2134
                     /locus_tag="VF225_04435"
     CDS             911..2134
                     /locus_tag="VF225_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144535.1"
                     /translation="MRVALTNFRWPLDPALVEGRDETTLARVLYSTPLRTDPATGAVV
                     PGLCTAWKASPDFRRWTFSCRSAPSIAAAVKRVVRLRRAPARWLFAGARQIVAPSASS
                     LVIQLDHPWRRLPYALTAVAAAPRFVPGPFRLVSGSSLRVVVRRPGLTVVFRRLAALT
                     ALDEFRRGRLDEAPVPLGDIVALQRDPVLGASLRARRLLAQDLVVFRGGPSQSLIHAY
                     WDTADRSDYEQLVPELPGSAAFGLVDPGEQRDPARFRDTLKRIPSLPRVQVRIGVPSD
                     PVLRFGARVLYAEWRNVGLGPVLVADSARNVTTDFTRLVAAYPQAEALLAEAVHLSDF
                     GRRDLLAAVLASTEQGPALERLDAALRALGRVVPVAWVVDARLVSSRLEGWREDVLGN
                     VDYAEVRSRASSQRP"
     gene            complement(2103..3575)
                     /locus_tag="VF225_04440"
     CDS             complement(2103..3575)
                     /locus_tag="VF225_04440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7144536.1"
                     /translation="MATLRDDQLRQRSARSERARLARRCSGAFSQRAGRGRVLNALEV
                     AKAALDTVEGEAEAVAHVEHSGLARFAGSEVHQPTLIENALVTLRVVHDNRVGVATTN
                     KLDAAGLAELARRAADAAESAPPDETFPGLAPPADPPAVEGYDEETAALSPADQARLA
                     AAAIDAGGDVPVYGFFTSAVSELAATSTTGLSVHQRMTDATALVVAADEHGSGYAEQT
                     AWRVGVIDPSAVAGAAADKARRTRGAAEIDPGVYRVVLEPYAFADLLDYFSHDTFGAL
                     GLLDKRSYFADRLGQKVFDEKISITDDALDPRGLPKAFDFEGTPKRPVQLVEQGVARG
                     VVWDRATAAQAGEGAESTGHAAPAELRDWGPLPSALSVLPGDAESVAELAELVGDGLY
                     ITRLHYLGVVHPREGIITGMTRDGTFRIRDGKIAEPLVNLRFTIAVPDFLRDVYGLTN
                     KAALVNSQNFYGERYPYGVMAPAIASERFPVTGVGSKPGI"
     gene            complement(3458..4891)
                     /locus_tag="VF225_04445"
     CDS             complement(3458..4891)
                     /locus_tag="VF225_04445"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7144537.1"
                     /translation="MRDLCATAVDAATGAGAEYADARVVRKRNQFVATKSGRVERLSD
                     SESEGIGVRVLLNGAWGFAADRRLSAAGARDAALRACTFARAAPGKHSRQLAPVAPAS
                     GTHRTPVERDPFEVPLAEKVAQCLEADEAMSGPGILVRQTMVRAQREHKLLLTSEGTE
                     VEQELIECGAGIECAAARDGLFQMRSYPSAHVGSSCQNGWEYVEGLDLAGQAPRVAEQ
                     ATALVRADECPSRVTTVVLDADQVALQVHESVGHPTELDRVYGTEASYAGTSFLKPSD
                     LGSLRYGSELMNITADPTTHGGLGSFAFDDEGVPAERTPVVEAGLLTGFLTSRETAAR
                     IGAGNGGSMRADGWNRMPLVRMTNLHLEPGEGTLEDLLADVDDGVYMETNRSWSIDDK
                     RLNFQFGTQIAWEIKRGKLGRMLRDATYTGITPQFWGSLDAVAGRDSWRLYGMTNCGK
                     GQPGQSAHVSHGAAPARFRNVQVGVGS"
     gene            4943..5028
                     /locus_tag="VF225_04450"
     tRNA            4943..5028
                     /locus_tag="VF225_04450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4977..4979,aa:Leu,seq:gag)
     gene            5226..5837
                     /gene="lexA"
                     /locus_tag="VF225_04455"
     CDS             5226..5837
                     /gene="lexA"
                     /locus_tag="VF225_04455"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7144538.1"
                     /translation="MLTGRQQEIWDFLVDYVDTHGYPPTVREIGEAVGLASPSTVHAH
                     LANLERAGLLKRDPTKPRALELVGRERHEAPAATLEKLPLVGQIAAGGPLLAEENVED
                     HLAVPETLKGDFLLRVKGESMINAGILDGDLVVVQRAQDARNGEIVVALAGDDESADE
                     ATVKRFFRENGKVRLQPENDALEPIYASHVQILGRVTGVFRSL"
     gene            5834..6037
                     /locus_tag="VF225_04460"
     CDS             5834..6037
                     /locus_tag="VF225_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144539.1"
                     /translation="MSSVAPLHRTLEQELQALLRGASMECLVCGEFVLHAGGMVTCPE
                     CNSSLRAELGVEETQLQFDTQAG"
BASE COUNT          916 a         2135 c         2099 g         1037 t
ORIGIN      
        1 gccggcctcg cgggccgcct cgagcgcctg ctcgtagctc tgccgcgcct tgacggggtc
       61 gacgccctgc ttgagccggt tgtctccggt gacgaggagc ttctgaatcc gctcgcgtgc
      121 ctcgtccccc ttcacaaatc gaccgtaaca gttcacattg ggggtggaac cgcgtcatgg
      181 aggccgccgc caacgagcca gaccggatcc gcgttctgat cgcggacgac caccgtttgt
      241 tcggacaggc cctcgaggcc atcctcgcga ccgacgatcg gctcgaggtg gtgggacacg
      301 ccggggatgg gtcggaagcc gtccagctcg cgctttccgt gaagcccgag gtgatcctca
      361 tggatatctc gatgccggtc atggacggct ttcaggcgac gaaacagatc cgcaagcagc
      421 ggccacacgc gcgcatcctc atgctcaccg gctcgaactc gcgagtcgac gtcgaccgtg
      481 cgcgggaggc gggagcggcc ggatacgtga ccaagaatcg aattgcggcc gagttgatcg
      541 acgcgatcct cgaagtcgcc ggctaaccac actgctaccc tgccgtcgcg atggttcagg
      601 cgctcgccgt catccaggta ctgctttcga tgggccttgt cgccctgatc ctcatgcact
      661 ccggccggga cgcaggcctc ggcggaatgg gcttcacgcc cgcgtcacag ggtggcacgc
      721 acatcgtcga gcgcaacctc acgaggctga ccgtcgtcat cgccgtcctc ttcgtcacga
      781 actccatcct gctgtttcac gcgctcaagt agcagcgctc tgcgccgtcg tcgcgttgat
      841 cgcggcggga tgtggcgctg gtcaagcccc gcgcgcgctt cctcgcgaag cgaccggtcc
      901 gccgaacgtt gtgcgcgttg ctctgacgaa ttttcgctgg ccgctggatc ccgcgctcgt
      961 cgaaggccgt gacgagacaa cgctcgcgcg cgttctctat tcgactccct tgcggacgga
     1021 tcccgccacg ggagcggtcg ttccggggct gtgcactgcg tggaaggcgt cgccggactt
     1081 caggcgctgg accttcagct gccgatccgc tccctcgatc gcggccgcgg tgaaacgcgt
     1141 cgtgcggctg cgtcgcgccc cggcgcgctg gctcttcgcc ggggcacgcc aaatcgtggc
     1201 gccatcagcc tcgtcgctgg tgatccagct cgaccatcct tggcggcgtc tgccttacgc
     1261 gctgaccgca gttgcggcgg cgccgcgttt cgttcccggg ccgttccggc tggtgagcgg
     1321 ctcgtcgctc cgcgtcgtcg tgcgccggcc cgggttgacg gtcgtcttcc gccgactcgc
     1381 cgcgctcact gcgctggacg agtttcgccg cggccggctc gacgaagcgc cggtgccgct
     1441 gggcgacatc gtcgcgttgc agcgggaccc cgtcctcggc gcgagtctca gggcacgaag
     1501 gctgctcgca caggacctgg tcgtcttcag aggcggcccc tcccagtcgt tgatccacgc
     1561 gtactgggac acggcggacc gctcggatta cgagcagctc gtgcccgagc ttccaggttc
     1621 cgccgcgttc ggcctcgtgg atcccggcga gcaacgggac cccgcgcgct ttcgcgacac
     1681 gctcaaacgc atcccgtcac tcccgcgcgt gcaagtgcgg atcggtgtcc cgtccgaccc
     1741 cgtgctccgt ttcggcgcga gagtgctgta cgcggagtgg cgcaacgtcg gcctcggccc
     1801 ggtgctcgtc gcagattccg ctcggaatgt gacgaccgat ttcacccgtc tggtcgcggc
     1861 gtatcctcaa gccgaggctc tgctcgcaga ggctgtgcac ctctccgact tcggcagacg
     1921 tgacctgctc gccgccgtgc ttgcatccac cgagcaaggc ccggccctcg agcgtctcga
     1981 cgctgccctg cgggcgctcg gccgggtcgt cccggtcgcg tgggtggtcg acgcgcgcct
     2041 cgtctcgtcc cggctcgaag gctggcgcga agacgtgctc ggcaacgtcg actacgcaga
     2101 ggtcagatcc cgggcttcga gccaacgccc gtgaccggga agcgctcgga cgcgatcgcg
     2161 ggcgccatga ccccgtacgg gtaacgctcg ccgtagaagt tctgcgagtt cacgagcgcg
     2221 gccttgttcg tgagcccgta gacgtctcgc aggaaatcgg gcacggcaat cgtgaaacgc
     2281 aggttcacga gcggttcggc gatcttgccg tcgcggatgc ggaaggtccc gtcgcgcgtc
     2341 attccggtga tgatcccttc gcgcggatgc acgacgccga gatagtgcag gcgcgtgatg
     2401 tagaggccgt ccccgaccag ctcggcgagc tcagcgaccg attcggcatc gcccgggagc
     2461 acggagagcg cagaagggag agggccccag tcgcgcaatt cggcgggcgc tgcgtgaccg
     2521 gtcgactcgg cgccttcgcc ggcttgcgcg gccgttgccc gatcccagac gacaccgcgg
     2581 gcgacgcctt gctccacgag ctgcacgggc cgtttgggcg tgccttcgaa gtcgaacgcc
     2641 ttcggcagcc cgcgcggatc gagcgcgtcg tcggtgatgg agatcttctc gtcgaagacc
     2701 ttctggccga gccggtctgc gaagtagctg cgcttgtcga ggagcccgag cgccccgaac
     2761 gtgtcgtggg agaagtagtc gagcaggtcc gcgaacgcgt acggctcgag caccacgcgg
     2821 tacacacctg gatcgatctc ggctgcgcct ctcgttcgcc gcgccttgtc tgcggccgca
     2881 cccgcgacgg cgctcggatc gatcacgccc acgcgccagg cggtctgttc ggcgtagccg
     2941 gatccgtgct cgtctgcagc gacgacgagc gccgtcgcgt ccgtcatccg ctgatggacc
     3001 gaaagtcccg tcgtggacgt ggccgcaagc tcggagacgg cgctggtgaa gaagccgtag
     3061 acggggacgt cgccgcccgc gtcgatcgct gcggctgcca ggcgagcctg gtcggccgga
     3121 ctgagcgcag cggtctcttc gtcgtagccc tccaccgcgg gtgggtcggc gggaggtgcc
     3181 aaccctggaa aggtctcgtc gggcggcgca ctctcggcgg cgtcggccgc ccggcgtgcg
     3241 agctccgcca gcccggccgc gtcgagcttg ttcgtcgtcg cgacgccgac gcggttgtcg
     3301 tgcacgactc gcagcgtgac gagcgcgttc tcgatcagcg tcggctggtg cacctcggag
     3361 ccggcgaagc gggctagccc tgagtgctcg acgtgcgcga cggcttccgc ctcgccctcg
     3421 accgtgtcga gtgccgcttt cgcgacttcg agtgcgttca ggacccgacc ccgacctgca
     3481 cgttgcgaaa acgcgccgga gcagcgccgt gcgagacgtg cgcgctctga ccgggctgac
     3541 ctttgccgca gttggtcatc ccgtagagtc gccacgagtc gcgtcccgca acggcgtcga
     3601 gggagcccca gaactgtggc gtgattccgg tgtacgtcgc gtcgcgcaac atgcgtccga
     3661 gcttgccgcg cttgatctcc caggcaatct gcgtgccgaa ctgaaagttg aggcgcttgt
     3721 cgtcgatcga ccagctgcgg ttcgtctcca tgtacacgcc gtcgtcgacg tcggcgagca
     3781 gatcctcgag tgttccctcg ccaggttcca ggtggaggtt cgtcatgcgc acgagtggca
     3841 tccggttcca gccgtccgcc cgcatcgatc cgccgttgcc tgccccaatg cgcgccgcgg
     3901 tctcgcgtga agtgaggaag cccgtgagca gaccggcctc cacgaccggc gtgcgctcgg
     3961 ccgggacgcc ttcgtcgtcg aacgcgaagc tgcccagtcc tccgtgggtc gtcgggtcgg
     4021 cggtgatgtt catcagctcc gagccatagc ggagggagcc gaggtcgctc ggcttcagga
     4081 agctcgtgcc ggcgtatgag gcctcggtgc cataaacccg gtcgagctcg gtgggatgac
     4141 cgaccgactc gtggacctgc agcgcaacct ggtccgcgtc gagcacgacg gtcgtcacgc
     4201 ggctcgggca ctcgtccgcg cgcacgagtg cggttgcctg ctcggcgacg cgcggcgcct
     4261 ggccggcgag gtcgagccct tcgacgtact cccagccgtt ctggcagctc gagccgacgt
     4321 gcgcgctcgg ataactccgc atctggaaga ggccgtcgcg ggcggctgcg cactcgatcc
     4381 ccgcaccgca ctcgatcaac tcctgttcga cctccgttcc ttcggaggtg agcaggagct
     4441 tgtgctcccg ctgcgcgcgc accatcgtct ggcgaaccag gatccccggg ccggacatgg
     4501 cttcgtccgc ttcgaggcac tgcgcgacct tctccgcgag gggaacctcg aacgggtccc
     4561 gctcgaccgg cgtgcggtgc gtcccgctcg cgggagcgac gggggccagt tgccgggagt
     4621 gcttgcccgg cgctgctcgt gcaaacgtgc aggctcgcag cgcggcatct cgcgccccgg
     4681 ccgcagagag cctgcggtcg gccgcaaagc cccaggcgcc gttcagcagc acccgcacgc
     4741 cgatgccctc gctctcggaa tccgacagac gctcgacacg gccgctcttc gtcgcgacga
     4801 actggttcct cttcctcacg acgcgcgcgt ccgcgtactc ggcccccgcg ccggtcgccg
     4861 catccacggc ggttgcacac aggtctcgca tccgcgcggc actctaatcg cgagaccacg
     4921 gctacaatgc gccggccttc aagcgctggt ggcggaattg gtagacgcgc taggttgagg
     4981 gcctagtggc cttaaaaggc cgtggaggtt caagtcctct ccagcgcact ctcaccaccg
     5041 aagtccggcc ccgctccgcg gggccatggt gacttcggtg gtatcggcgt cgatccgacg
     5101 ccgtagcggc ggacgcgggc caagcccgca tccgcggcga gtcgggtttg acaacgtccg
     5161 ggaggatctc tagcctggat ggcgaacaca cgttcgatcg accatccaac ccttggaggc
     5221 ggagcatgct gacgggacga caacaggaga tctgggactt cctcgtcgac tacgtcgaca
     5281 cacacggcta cccgcccacc gtgcgggaga tcggcgaggc agttgggctg gcctcgcctt
     5341 cgacggtgca cgcccacctc gccaacctcg aacgagccgg attgctgaag cgcgacccga
     5401 cgaagcctcg agcgctggag ctcgtcggcc gtgagcgtca cgaggcgcct gcggcgactc
     5461 tcgagaagct gccgctcgtc ggccagatcg ctgccggcgg accgctcctc gccgaggaga
     5521 acgtcgagga tcatctggcc gtgcccgaga cgctcaaggg tgacttcctc ctgcgggtga
     5581 agggcgaatc gatgatcaac gcgggcatcc tcgacggcga cctcgtcgtc gtgcagcggg
     5641 cgcaggacgc gcgcaacggg gagatcgtcg tggccctcgc cggcgatgac gagtcggccg
     5701 acgaggccac cgtcaagcgc ttcttccgcg agaacgggaa ggtccggcta cagcccgaga
     5761 atgacgccct cgagccgatc tacgcgtcac acgtccagat cctcgggcga gtcacggggg
     5821 tgttccgctc gctgtgagct cggtcgcgcc tctccaccgg actctcgagc aggagctgca
     5881 ggcgctgctc cgtggcgcca gcatggagtg cctcgtctgc ggcgagttcg tgctgcacgc
     5941 gggtggcatg gtcacctgcc cggagtgcaa ttcgagcctg cgcgcggagc tcggagtgga
     6001 agagacgcag ctacaattcg acacgcaggc cgggtgaccg cgggccacgg ctcgaggaaa
     6061 gtccggacac cgcagggcag gacgctgggg cgacccaggc ggcgaaagcc gacggaaagt
     6121 ggcacagaaa ggagaccgcc tgcagcgatt ctccggagac gctgaaggta agggtgaaaa
     6181 ggtggag
//
LOCUS       DASVEU010000114        14543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1630078,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000114 DASVEU010000000
VERSION     DASVEU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14543
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630078"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF225_04465"
     CDS             complement(<1..521)
                     /locus_tag="VF225_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144540.1"
                     /translation="MSLQQPDFGVLRKAQRGDERAFSLIVRAYQVPVFNYVLRLVGDR
                     SLAEDLTQEVFLRVFQGLPRFSLRSKFTTWLFQVTKNRVLDELRANERRPRAVVALED
                     VPSLEVVDAPMERLEAIDAVWRAVEGLNVDLKMALLLRDVVGLSYTEIADSLEITLAT
                     VKWRIYKAREDVQL"
     gene            complement(545..2005)
                     /gene="gpmI"
                     /locus_tag="VF225_04470"
     CDS             complement(545..2005)
                     /gene="gpmI"
                     /locus_tag="VF225_04470"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX7144541.1"
                     /translation="MPSGREHPLVALVILDGWGLAPAGPGNAVELADTPNFDRLWKTF
                     PHTTLDASGEAVGLPPGQMGNSEVGHLTIGSGRVLFQDLMRVNKSIEDGSFFQNPALV
                     SAFERGEQVHLLGLVSYGGVHSHIDHLRALLRFAPEKTWIHAFTDGRDVSPHAAVDDL
                     AELPTERISTVSGRYYAMDRDQRWERTQRAFDAIVEGKGEQATDPIEAVRRSYERGIT
                     DEFIEPTTVGGRPRLQPGTDTAIFFNFRPDRARQLSMKLVEAGADITTMTRYRADLEV
                     PVAFPEQEVRETLAEVLAEHGVRQLHAAETEKYAHVTYFFNGGREAEWEGETRILVPS
                     PRDVASYDQKPEMSAREVTDRFVAELDRNGYRFAVINLANPDMVGHTGSIPAAVKAVE
                     TADECLGRIVEAVARKGGVSLITADHGNAEQMLEADGASPHTAHTTNPVPLIVTDPEV
                     TLDGTGELSDLVPTLLGFLGFTQPLQMTGKPLTTPD"
     gene            complement(1975..2682)
                     /gene="tpiA"
                     /locus_tag="VF225_04475"
     CDS             complement(1975..2682)
                     /gene="tpiA"
                     /locus_tag="VF225_04475"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7144542.1"
                     /translation="MSLIAGNWKMYKGAAETAEFCLGLREEELEGVDVVVCPPFVSLA
                     VAVQLLAGTEIAVAAQNVHWEEEGPYTGEISAGMLLELGVYGAIVGHSERRQYFGETD
                     ETVGDRVRAALEAGMFVIACVGETEGERERGQTESVLQRQLSAFDADDNLVLAYEPVW
                     AIGTGKTATPEIAQEAHAFIKSILGVPVLYGGSVKPDNAAVLLSQPDVDGALVGGASL
                     DLESFISICRAGASIPS"
     gene            complement(2693..3736)
                     /locus_tag="VF225_04480"
     CDS             complement(2693..3736)
                     /locus_tag="VF225_04480"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7144543.1"
                     /translation="MHRPRSVRDADIAGKRVLVRSDLNVPLAGGRIADETRIQGSLPT
                     LQLLLERDAGEVTVCSHLGRPKGEDPAFSIRPVAERLQELLPDDRLRVLENTRFNPGE
                     TKNDPGFAQELADDNDLFVQDAFGSVHRAHASTVGVAQLLPTYTGLLLERELTELGKL
                     LGDVERPFVLISGGAKVDDKLGVLKNLGGKADAVLVGGKMAEQIRTDNPLPFDVVLPV
                     DIVAASEFAADAEAKVVPYDGLPEGWLGLDIGPETRQLFAQHISQARTIFWNGPMGVF
                     EWPRFAQGTKAIAEAVASSGGYSVVGGADSARALSELGLADKVSWLSTGGGAALELLE
                     GKDLPGVSVIPAS"
     gene            complement(3737..4768)
                     /gene="gap"
                     /locus_tag="VF225_04485"
     CDS             complement(3737..4768)
                     /gene="gap"
                     /locus_tag="VF225_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7144544.1"
                     /translation="MANMTRVGINGFGRIGRNFFRAQLERGGDFEIVAINDLGDAKTM
                     GHLLKFDSVLGQLSEDVEVGDGVIRAGKSELKVLSERDPAALPWGDLEVDLVIESTGF
                     FTDREGAQKHLDAGAKKVVISAPATDPDVTIVLGVNDDTYDPEQHHIISNASCTTNCV
                     APLAKVLHDNFTVEQGFMTTIHAYTNDQQILDLPHKDLRRARAAAINLIPTSTGAARA
                     IGLVLPDLKGKVDGISVRAPVPTGSITDLVVRLGREVTVDDVNSAYRAAADTGSFEGI
                     LRYADDPLVSTDIVHSPYSCIVDSELTMANGTMAKVFGWYDNEWGYSCRLVDVVAKIA
                     ADIPATVSA"
     gene            complement(5084..6004)
                     /gene="whiA"
                     /locus_tag="VF225_04490"
     CDS             complement(5084..6004)
                     /gene="whiA"
                     /locus_tag="VF225_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX7144545.1"
                     /translation="MSLSEDLRHELAAITPRSECDRLAELSGLFHSAGSVHLKGRGEV
                     SVHLDVGDPAVTRRAFSLLRAFGVVSEIRTYPRHAFDGGTRYQLHVAGDDHALQVLHE
                     AGVLSSRLTPLGRPPRRVVARSCCRAAYLRGALLGGGSLSGPRSPHLEIRSAEIDGAE
                     FLARTAGEEGVRLGVLDRGRHAVAYAKGIETIADALALAGASRTVLLFEERAVMAATR
                     SRANRLANADHANLVRTSRAAHDQLRAVERLRRSGELAKLPARLQEIAELRERHPTLS
                     LRELALKCRPPATKASAHRRLRKLQEFAST"
     gene            complement(5991..6356)
                     /locus_tag="VF225_04495"
     CDS             complement(5991..6356)
                     /locus_tag="VF225_04495"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7144546.1"
                     /translation="MGNPSAPKAEKLIDRFRTYFAGERASFVDVRLEFEGWTTFQLDV
                     VRALRRVPYGEVVSYGELARLAGHPRAHRAAGTFCARNRFGIVVPCHRVVASDGLGSY
                     GSLGTDYKQRLLALEGVTL"
     gene            6584..8239
                     /gene="pruA"
                     /locus_tag="VF225_04500"
     CDS             6584..8239
                     /gene="pruA"
                     /locus_tag="VF225_04500"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX7144547.1"
                     /translation="MSAAPGLFRPPMPENEPVKSYAPGTPEREELRLRLQQMQSEQIE
                     APLVIGGEEVRTNETFETVMPHDRDHVLATVHKGDARHVEQAIKAAAEAWEDWHRAPW
                     EDRAAVFLRAAELLAGPWRSTLNAATMLGQSKTAHQAEIDAACELTDFFRFNVQFMLR
                     IYGEQPISSPGTWNRMEYRPLEGFVFAVTPFNFTAIGGNLPGSAALMGNTVVWKPAST
                     AAYSAHFLMKLFEAAGLPPGVINLVYGSGAEIGDPALASPDLAGVHFTGSTPVFQSMW
                     KTIGNNITNYRNYPRIVGETGGKDFIVAHPSADAAAVATAIVRGSFEYQGQKCSAASR
                     IFAPSNLWPEIRERVVEEVRSIKMGDVSDFENFMGAVIDAGSFRTQREAIEEAKAADK
                     VDVLVGGGYDDSEGYFVEPTVLETTDPNFRTMREELFGPVVTTYVYPEGKYGETLDLI
                     DKGAPYGLTGAVFARERDAVDLADEKLRYAAGNFYVNDKPTGAVVGQQPFGGARASGT
                     NDKAGSMWNLIRWVSPRTIKETFVPPTDYRYPFMAPEDGREPG"
     gene            8244..9548
                     /locus_tag="VF225_04505"
     CDS             8244..9548
                     /locus_tag="VF225_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144548.1"
                     /translation="MKPLLLIASAFAAALLVVPAAEAGPIIDRAVAALHSDPVYVDPA
                     AELAPSAADQDRLRTKIDRAGAGPVYIAILPNAAELEAGGSPDGVLQAIHTGLRLKGT
                     YAAVIGTHFRAGSDGVLPKGVAGLLATEALAAHRGAGVTATLLDFIDRVAAERRGGAG
                     GGGGQPSGAGGVVIALLIAAVVAFLLFQAVRRRRRSQDEVAEVKAAAHDDLVSLADDV
                     QKLEQPVESNPQAKQEYTQALDSYDKASGSYDRATEPRQLEAVATALEEGRWHMSAAQ
                     ALLDGHAPPERRPPCFFDPRHGPSTRDVDWAPPGGTPRKVPACEADALAVEAGAQPAS
                     REVLTGGRKVPYWSAPPYFGPWAGGYFMPFGGTGFLSGLFVGELLGGAWGGWGYGSWG
                     GAGTAGGFGNWNDAGGGKGDFGGGMSFGGGDFGGGGGGGGDF"
     gene            9573..10523
                     /locus_tag="VF225_04510"
     CDS             9573..10523
                     /locus_tag="VF225_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7144549.1"
                     /translation="MIHSVLHAIGVFFHHLAAVEWKWLAIGILCHLCKLLAVSRAWRN
                     IVHAAYPDRPVRWRQMFGAYVAGTGVNAVIPARGGDVVRLFLAKRRIDGSTYTTLVST
                     SLLQTLFDMLVASCFIVWAVTQHVLPGLDALRSPSLPSLDYGWAFRHPLAGLILVGLL
                     LIFGTALVAWVAERVDEFQAKVAQGFAAFRDRSYYLRRVVLWQLVDWSLRLATVFFFL
                     RAFGIPATLRNALLVQVSQSLATIFPVSPAGIGTEQALLLYIFRNVTSKSLSLSFSVG
                     MRVTLIIVNAIVGFTAILLMTGTLRVRRTAQADRAGGEPA"
     gene            complement(10729..12063)
                     /locus_tag="VF225_04515"
     CDS             complement(10729..12063)
                     /locus_tag="VF225_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7144550.1"
                     /translation="MEGRILTPPRAPQRTTSGLGTRLRQLRVAAGLTQSELAGERFSK
                     EYMSQIERGKTRPTKETLSWIGERLGVDVSYLETGQSWDEYAEVEAAITRAEAAVEGQ
                     QYDEALEALAGLEFSPEARELEFRALLAEAWARMAGAELRPALELLVRARELAEQAVF
                     SDVERAEVLYRMGVCRYKLTSINTAQTLLSEALILADRSGDPCDRLRSHILEWRSRCS
                     RRQRDFEAAREDIERALELAERLDDTLTVAHITFQASIVAERDGHWLRARSLAERAKD
                     LYEQHGDRQNLGRMLNNLGGLNYLLGKPDDAISYLKRAFSVALEIGSDADAAQAVSSL
                     AQVHLGAGDWSLAEEQSRHALELLAGRPDFLDEIGNAQIVLGRALLEQQRLDEAEHAF
                     AEAELSLSQLSSASHRAVAWTAQGDLASRRGDDSAAAALYRRAAEALQDVRF"
     gene            complement(12146..13996)
                     /gene="uvrC"
                     /locus_tag="VF225_04520"
     CDS             complement(12146..13996)
                     /gene="uvrC"
                     /locus_tag="VF225_04520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7144551.1"
                     /translation="MASRLENQLKGLPAKPGVYLFRDRAGEVLYVGKAKSLRPRVRSY
                     FQQSHDTRTAIQQLPERVEDIEVIVTQSEVEALHLEQNLVKRHRPPFNVRLRDDKSFP
                     YIAVTVADEYPRVMFTRERHRRGVVYFGPYANAKKVRETLDVLNRVFPYRPCEGPKPG
                     RHSGIPCLDYHIERCLAPCVGYVSKEDYRAIIDQVIEFLSGDTEPIQRELERKMQAAA
                     ADERFEEAARYRNRLRSVQHLAERQAADLRSVGTIDVIGLALEADRAVVQVFPLRDGK
                     MIDRYSFHLENVGGQDLGTVLESFFLEYYGSAPSVPPQIVVPRESGDTSALAEFLSER
                     RGARVEVRAAERGEKRRLQELATENARHALAAEAEQTELKRLRRVEALEELREVLNLE
                     ALPIRIECFDISTVMGQDNVGSMVVFQDGVAKKAHYRKFGIKGQVGMDDFAAIAEVVS
                     RRFSRLADVTSEEYDQSFAATPNLVVIDGGKGQLSAALAAMQAFDLPRVAVVALAKRV
                     EEVFVPGSPDPIVLAAHSPGLQLLQRVRDEAHRFAVGFHRQRRDARSFASIFDELEGV
                     GPARRRALLQHFGSAERFLAASQEELEGVPGVPAKTARSIYAQLHKAGRA"
     gene            14310..>14543
                     /locus_tag="VF225_04525"
     CDS             14310..>14543
                     /locus_tag="VF225_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144552.1"
                     /translation="MLKPRSLIALLCAFAALAAPTHALGSERMYMGAAEDEGRNADPA
                     VAMAKMELAKAAGFDTIRITAVWEPGQSAVPAGQ"
BASE COUNT         2392 a         4871 c         4824 g         2456 t
ORIGIN      
        1 agctggacgt cctcgcgtgc cttgtagatc cgccacttga ccgtggcgag tgtgatctcg
       61 agggagtcgg cgatctcggt gtacgagaga ccgacgacgt cgcggaggag cagcgccatc
      121 ttcagatcga cgttcaatcc ctcgacggca cgccacaccg cgtcgatcgc ctcgagccgc
      181 tccatcggag cgtcgacgac ctcgagcgac ggaacgtctt cgagcgcaac gaccgcccgc
      241 gggcgccgct cgttcgcacg cagctcgtca agcacgcggt tcttcgtcac ctggaaaagc
      301 cacgtggtga acttcgagcg aagcgagaaa cgcggcaggc cctgaaagac ccgcaggaag
      361 acttcctgag tcaggtcctc tgccagcgac cgatcaccga caagtcgcag gacatagttg
      421 aagacaggca cctgataggc acggacgatg agcgagaaag cccgctcatc gcctctctgt
      481 gccttccgca ggacgccaaa atcaggttgc tgaagtgaca aaaggttccg gagtatagcc
      541 cggatcagtc cggggtcgtt aagggtttac cggtcatctg taagggttgc gtgaaaccta
      601 aaaaccccag caaagtgggc actaaatcag acagctcgcc ggtgccgtca agggtcactt
      661 cggggtccgt gacgatgagc gggacggggt tcgtcgtgtg cgcggtatgg gggcttgcac
      721 cgtctgcctc gagcatctgt tcggcgtttc cgtggtctgc ggtgatcagc gagacgccac
      781 cctttcgggc gaccgcctcg acgattcgac cgaggcactc gtcggctgtt tccaccgctt
      841 tcaccgccgc ggggatcgat cctgtgtggc cgaccatgtc ggggttcgcc aagttgatga
      901 cggcaaaccg gtagccgttt cggtcgagct ctgcgacgaa tcggtcggtc acttcccgcg
      961 cggacatctc cggtttctgg tcataactcg caacatcccg cggcgacggc acgagaatcc
     1021 gggtctcccc ctcccactcc gcctcgcggc cgccgttgaa gaagtaggtc acgtgtgcgt
     1081 acttctcggt ctcggcggcg tgcagctggc gtaccccgtg ctcggcaaga acctcggcca
     1141 gcgtctcccg cacctcctgt tccgggaaag cgaccggtac ttcgagatcc gcgcggtatc
     1201 tcgtcatcgt cgtgatatcg gcacctgcct cgacgagctt catgctcaat tggcgggctc
     1261 tgtcaggacg gaagttgaag aagatggctg tgtccgtccc cggctgaagc cgcggccggc
     1321 caccaactgt ggttggttcg atgaactcgt cggtgatccc tcgctcatag ctacggcgga
     1381 cggcctcgat cggatcggtt gcctgctcgc ctttcccctc gacaattgca tcgaaggcgc
     1441 gctgtgtccg ctcccagcgc tggtcgcgat ccatcgcgta gtaccggccg ctgacggtgg
     1501 agatgcgctc cgtggggagt tcggcgaggt cgtcgacggc ggcgtgcgga gaaacatccc
     1561 gaccgtctgt gaacgcgtgg atccaggtct tctcgggagc gaagcgcaac agcgcgcgga
     1621 gatggtcgat gtgcgagtgg acaccgccat atgagacgag gccgagcaga tgcacctgct
     1681 cgcctcgttc gaaggcgctc accagggccg ggttctggaa gaacgacccg tcttcgatcg
     1741 acttgttgac gcgcatgagg tcctggaaca ggacgcgtcc ggagccgatc gtgaggtgtc
     1801 cgacctcgct gttgcccatc tgtccgggcg gcaggccgac ggcctcgccc gacgcgtcga
     1861 gcgtcgtgtg ggggaacgtc ttccacagcc ggtcgaagtt cggcgtgtcg gcgagctcca
     1921 cggcattgcc cggtccggcc ggcgcgaggc cccagccgtc gaggatgacg agcgctacga
     1981 ggggatgctc gcgcccgctc ggcaaatcga gatgaaggac tcgaggtcga gcgacgctcc
     2041 gccgacgagc gcgccgtcga cgtcgggctg gctcaggagg accgcggcgt tgtccggctt
     2101 gaccgacccg ccgtagagca cgggcacacc gagtatcgac ttgatgaacg cgtgcgcttc
     2161 ctgcgcgatc tccggcgtgg ccgtctttcc cgttccgatt gcccagacgg gctcgtacgc
     2221 gaggacaaga ttgtcgtcgg cgtcaaatgc ggagagctgc cgctgcaaga cgctctcggt
     2281 ctggccgcgc tcccgctccc cttccgtctc gccgacgcac gcgatgacga acatacctgc
     2341 ctccagtgcg gctcgtaccc tgtcccccac ggtctcgtcc gtctcgccga agtactgccg
     2401 gcgttccgaa tgcccgacga tcgcgccgta gaccccgagc tcgagaagca tcccggccga
     2461 gatctctccc gtgtaggggc cctcctcttc ccagtgcacg ttctgcgccg caactgcaat
     2521 ctccgtgccg gcaagcaact ggactgcgac cgcgagcgag acgaatggcg ggcagacgac
     2581 cacatcgacc ccctcgagct cttcctcgcg gagccccaga cagaactccg ccgtctcggc
     2641 cgcgcccttg tacatcttcc aattccctgc gatcagtgac aagtgtcaga tcttacgacg
     2701 caggaatcac gctgacgccg ggcagatcct tcccttctag aagctctaga gcggcgccgc
     2761 cgccggtcga gagccaggaa accttgtcgg ccagcccgag ctcgctcagc gcgcgtgccg
     2821 aatcggcgcc gccgacgacc gagtagccgc cggacgaggc gaccgcctcc gcgatcgctt
     2881 tcgttccctg ggcgaaccgc ggccactcga acacgcccat cggtccgttc cagaagatgg
     2941 tgcgtgcctg cgagatgtgc tgcgcgaaca gctgcctggt ttcgggtccg atgtcgagcc
     3001 ccagccagcc ttccgggaga ccgtcgtagg gcacgacttt cgcctcggcg tcggcggcga
     3061 actcgctcgc ggcaacgatg tcgaccggca ggacgacgtc gaacggaagc ggattgtccg
     3121 tcctgatctg ctcggccatc ttgccgccga cgagcaccgc gtcggccttt ccgcccaggt
     3181 tcttcagcac gccgagcttg tcgtcgacct tcgcacctcc cgagatgaga acgaagggcc
     3241 gctcgacgtc gccgaggagc ttcccaagct ccgtcagctc gcgttcgagc aagagcccgg
     3301 tatacgtggg aagcagctgc gcgacgccga ccgtcgacgc gtgcgcgcgg tgcacggatc
     3361 cgaacgcgtc ctgcacgaag aggtcgttgt cgtcggcgag ctcttgcgca aacccgggat
     3421 cgttcttcgt ctcacccggg ttgaagcgcg tgttctcgag cacgcgcagg cgatcgtcgg
     3481 gcaggagctc ttgcagccgt tcggcgaccg gccggatcga gaacgccggg tcttcccctt
     3541 tcggacgtcc gagatgggag cagaccgtca cttccccggc gtcgcgctcg aggagcagtt
     3601 gcagggtcgg cagggatccc tgaatgcgcg tctcgtccgc gatccgccca cctgccagcg
     3661 ggacgttcaa atcagaccgg acgagcaccc gcttgccggc gatgtcggca tcgcgtaccg
     3721 accgcggcct gtgcacctat gcggagacgg tcgccgggat gtcggcggcg atcttcgcca
     3781 cgacgtcgac gagacggcag ctgtagcccc actcgttgtc gtaccagccg aacaccttgg
     3841 ccatggtccc gttcgccatc gtcaattcgc tgtcgacgat gcacgagtag ggcgagtgca
     3901 cgatgtcggt cgagacgagt ggatcgtccg cataccgaag aatgccctcg aaggagcccg
     3961 tgtcggcggc agcgcggtag gccgagttca cgtcgtcgac ggtcacctcg cggccgagac
     4021 ggacgaccag gtccgtgatc gagccggtcg ggaccggtgc ccggacggag atcccgtcga
     4081 ccttgccctt caagtcgggg aggaccaatc cgatcgcacg ggcggcgccc gtcgaagtcg
     4141 ggatgagatt gatcgcggct gcgcgtgcac ggcgcaggtc cttgtgcggg aggtcgagga
     4201 tctgctgatc gttggtgtac gcgtgaatgg tggtcatgaa tccctgctcg accgtgaagt
     4261 tgtcgtgcag cactttggcg agcggcgcaa cgcagttcgt ggtgcacgac gcgttcgaga
     4321 tgatgtgatg ctgctcgggg tcgtaggtgt cgtcgttcac gccgagcacg atggtcacgt
     4381 cagggtcggt cgccggtgcg gagatgacga ccttcttggc cccggcgtcg agatgcttct
     4441 gcgcgccctc gcggtcggtg aagaagccgg tcgactcgat cacgaggtcg acctcgaggt
     4501 cgccccacgg cagcgcagcc gggtcgcgct cggaaagcac cttcaactcg gacttgccgg
     4561 ccctgatgac gccgtcccca acctcgacgt cttcgctcag ctgtccgagg acggagtcga
     4621 acttgagaag atgccccatc gtcttggcgt cgccgaggtc gttgatagcg acgatctcga
     4681 aatccccacc ccgctcgagc tgcgcccgaa agaagttcct gccgatccgc ccgaatccgt
     4741 tgatccccac gcgtgtcatg ttggccatgc gcgcagtcta aatgagccac tgccgatgcc
     4801 ggcttcgccg gcgtcggccg ggaacagctc gcgcccttgg gaacgaaacc gtcggaaatc
     4861 atcatggcgc gcgaagcgcg ccggatttcc gacggtgagc gccggatttt tgacgatgag
     4921 gcgggccgga ggagaagctc cgaggacgac ctcctcctcc ggttgaacca cgctcaggac
     4981 cttggcgtcg gacggggtct gaatccggcg ccggacgagc gcggacccgc caccagtggg
     5041 agtatgacga aagcttcgac atgacgacaa ccaggcgaga aagttaggtt gacgcgaact
     5101 cttgtaactt acgtaaacgc ctgtgcgccg acgctttcgt ggccggaggt cgacacttca
     5161 gggccagctc gcggagcgag agtgtcgggt gtcgctctcg gagctcggcg atctcctgca
     5221 gtcgagccgg gagcttggcc aactcgccgc ttcggcgaag tcgttctact gctcggagct
     5281 ggtcgtgtgc ggcacggctc gtccggacga ggttcgcgtg gtcggcgttc gccaggcgat
     5341 tcgcccgtga gcgcgtggcc gccatgaccg cgcgctcttc gaagagcaag acggtgcggc
     5401 tcgcaccggc aagcgcgagt gcgtcggcga tcgtttcgat tcccttcgcg taggcgacgg
     5461 cgtggcgtcc gcggtcgagc acgccgaggc gcactccttc ctcaccggcg gtgcgcgcga
     5521 ggaattcggc gccgtcgatc tctgcgctgc gaatctcgag atgcggcgag cggggcccgc
     5581 tgagcgaacc accgccgagc agcgctccgc ggaggtacgc agcacggcag cacgagcgcg
     5641 ccactacccg ccgtggcggg cgcccgagcg gcgtgaggcg ggagctgagc acaccggcct
     5701 cgtggagcac ctggagcgcg tgatcgtctc cggcaacgtg tagctggtac ctcgtgcccc
     5761 cgtcgaaggc gtgtcgcgga tacgtccgga tctcggacac gacaccgaac gcgcgcagga
     5821 gactgaacgc tcgacgcgtg accgccgggt cgccgacgtc gaggtgcaca gagacctcgc
     5881 cgcgcccctt gagatggacg ctacccgcag agtggaagag ccctgagagc tcggccagcc
     5941 gatcgcactc gctacgcggc gtgatcgccg cgagctcgtg ccgaagatcc tcagagagtg
     6001 acaccttcga gcgcgagcag ccgctgtttg tagtccgtac cgagagatcc atacgacccc
     6061 aggccgtcgg aagcgaccac gcggtgacag ggaacgacga tcccgaagcg gttgcgcgca
     6121 cagaacgtgc ccgccgctcg gtgagcccgt gggtgaccgg cgagccgggc gagttcgccg
     6181 taggaaacca cctccccgta cggcacgcgg cgcagggcgc gaacgacatc gagctggaac
     6241 gtggtccagc cttcaaactc gagccggaca tcgacgaagg atgcgcgctc gccggcgaag
     6301 taagtcctga acctgtcgat caacttctcc gctttcggcg cactcgggtt ccccatgcgt
     6361 gcgcgttttg gagttagcgt cgacctcgag ggcggtgggc gatcagcccc tccctgacgg
     6421 gtgggggttg tacgcgcacg cgcgcgtcta cctagagacg gctgcggaag ctcatgccag
     6481 acaaggcacc cgtcctcgag ccagacctcc cctacgcccc agccttcggc ctcgtagggg
     6541 atgcgcgtca cagacaccgt gcccaagcct acaatcgccc ccgatgtccg ccgcacccgg
     6601 cctgttccgc ccgccgatgc ccgaaaacga gccggtgaag tcgtacgcgc cgggcacgcc
     6661 cgagcgcgag gagctacgtc tccgcctcca gcagatgcaa tcggagcaga tcgaggcacc
     6721 gctcgtcatc ggcggcgagg aagtacggac gaacgagacg ttcgagacgg tcatgccgca
     6781 cgacagagac cacgtgctgg ccacggtgca caagggcgac gcgcgtcacg tcgagcaggc
     6841 gatcaaagct gcggcggaag cctgggagga ctggcaccgc gcgccgtggg aggaccgcgc
     6901 cgccgtcttc ctccgggccg cggagctgct ggccggtccc tggcgctcga cgctcaacgc
     6961 ggccacgatg ctcggccagt ccaagaccgc gcaccaggcg gagatcgacg ccgcttgtga
     7021 gttgaccgac ttcttccggt tcaacgtgca gttcatgttg cggatctacg gcgagcagcc
     7081 gatctcctcg ccgggcactt ggaacagaat ggagtaccgc ccgctcgaag gattcgtgtt
     7141 tgcggtcacg ccgttcaact tcacggcgat cggcgggaac cttcccggca gcgcggccct
     7201 gatggggaac acggtcgtgt ggaagcccgc gtcaaccgcc gcctactcgg cgcacttcct
     7261 gatgaagctt ttcgaggccg caggcctccc gcccggagtc atcaacctcg tctacgggtc
     7321 gggcgcggag atcggcgacc ccgccctcgc gagccccgac ctggccggcg tccacttcac
     7381 cggctcgaca cctgtcttcc aatcgatgtg gaagacgatc gggaacaaca tcacgaacta
     7441 ccggaactat ccgcgaatcg tcggcgagac gggcggcaag gacttcatcg tcgcgcaccc
     7501 ctccgccgac gccgcagctg tcgcgaccgc gatcgtccgc ggcagcttcg agtaccaggg
     7561 ccagaagtgc tccgcagcct cgcgcatctt cgctccgtcc aacctctggc cggaaatccg
     7621 ggagcgggtc gtcgaggagg tccgctcgat caagatgggg gacgtctccg atttcgagaa
     7681 cttcatgggg gcggtgatcg atgccggctc gttcaggacg caacgggaag cgatcgagga
     7741 ggcaaaggca gccgacaagg tcgatgtcct cgtcggtggc ggctatgacg attcggaggg
     7801 ctacttcgtt gagccgacgg tgctcgagac caccgacccg aacttccgca ccatgcggga
     7861 ggagctcttc gggcccgtcg tcacgaccta cgtctatccc gaagggaagt acggcgagac
     7921 gctcgacctg atcgacaagg gcgcaccgta cggactgacg ggcgcggtct tcgcgcgcga
     7981 gcgtgacgct gtcgatctcg cggacgagaa gcttcgctac gcggccggga atttctacgt
     8041 caacgacaag ccgaccggcg ctgtcgtcgg ccagcagccg ttcggcggcg cgcgcgcttc
     8101 cggcacgaac gacaaggcgg gctcgatgtg gaacctgatc cgttgggtga gccctcggac
     8161 gatcaaggag acgttcgtgc ctccgactga ctaccgctac ccgttcatgg cgcccgaaga
     8221 cggccgcgag cccggctagc tctgtgaaac cgttgctgct gatcgcgtcc gccttcgccg
     8281 cggcgctcct cgtcgtaccc gccgccgagg cagggccgat catcgaccgc gcggtcgccg
     8341 ccttgcactc cgatcccgtg tacgtcgacc cggcggcaga gcttgcgccc tcggccgccg
     8401 accaggaccg gctgcggacg aaaatcgacc gcgccggcgc cgggcccgtc tacatcgcga
     8461 tccttccgaa cgcggccgag ctggaagccg gaggcagtcc ggacggcgtc ctccaggcga
     8521 tccatacagg ccttcggcta aagggcacct acgccgccgt gatcggaacg cacttccgcg
     8581 ccggaagcga cggcgtcttg cccaagggcg ttgccggcct cctggcgacc gaggcgctcg
     8641 ccgcccaccg cggcgcgggc gtgaccgcca cgctcctcga cttcatcgac cgtgtcgcag
     8701 ccgagcgacg cggtggcgcc ggcgggggcg gcggccagcc gagcggcgcc ggcggtgtgg
     8761 tcatcgccct gctgatcgcc gcagtcgtcg cgtttctgct cttccaggca gttcgcaggc
     8821 ggcgccgctc gcaggacgag gtcgccgagg tcaaggccgc ggctcacgac gacctcgtct
     8881 ccctcgccga cgatgtccag aaactcgagc agcctgtgga atcgaatccg caggcgaaac
     8941 aggagtacac acaggcgctc gactcgtatg acaaggcgag cggctcgtac gaccgggcga
     9001 cggagccgcg tcagctcgaa gcagtggcga cggcgctcga ggaaggccgc tggcacatga
     9061 gcgctgcgca ggcgctcctc gacgggcacg cgccgccgga gcggcgtcca ccgtgcttct
     9121 ttgatcctcg ccacggcccg tcgacgcgcg acgtcgactg ggcgcctccc ggcggaacac
     9181 cgagaaaggt cccggcctgc gaggccgacg cactggccgt tgaagccggt gcgcagcccg
     9241 cctcacgcga ggtgctgacc ggcggccgga aggtcccgta ctggagtgct ccgccctact
     9301 tcgggccgtg ggccggcggc tacttcatgc cgtttggcgg aacgggattc ctgtccggcc
     9361 tcttcgtcgg cgagctgctg ggcggcgcgt ggggcggctg gggctatggc agctggggcg
     9421 gtgccggaac ggcgggcggc ttcggcaatt ggaacgatgc aggtggcggc aagggcgact
     9481 tcggcggcgg gatgagcttc ggcggcggtg atttcggggg cggtggaggc ggcggcggcg
     9541 acttctagcg gggcgtcggc aacactgcgt ccgtgatcca ctccgttctc cacgcgatcg
     9601 gagtcttctt ccaccacctt gcagcggtcg agtggaagtg gctcgcgatc gggatcctct
     9661 gccacctctg caagctcctt gcggtctcgc gggcttggcg gaacatcgtc cacgccgcgt
     9721 accccgaccg gcccgtgcgc tggcgccaga tgttcggcgc gtacgtcgca ggcaccggag
     9781 tgaatgcggt cattccggct cgtgggggcg acgtcgtgcg gctcttcctc gcgaagcggc
     9841 gcatcgacgg ctcgacctac acgacgcttg tctcgacctc gttgctgcag acgctcttcg
     9901 acatgctcgt ggcgagctgc ttcatcgtgt gggccgtcac gcagcacgtg ctgcccggcc
     9961 tcgacgcact tcgtagcccg agcctccctt cgctcgacta cggctgggcg ttccgccatc
    10021 ccctggccgg actcattctg gtcgggctcc tgctcatctt cggaacggcg ctcgtggcct
    10081 gggtcgccga gcgcgtcgac gagttccagg cgaaggtcgc gcagggcttc gcagcgtttc
    10141 gagaccgctc ctactatctg cggcgcgtcg tcctctggca gctggtcgac tggtcgctac
    10201 gccttgcgac cgtcttcttc ttcttgcgcg ccttcgggat accggccacg ctccgcaacg
    10261 cgctcctggt gcaggtgagc cagagcctcg ccacgatctt tccggtcagc ccggccggaa
    10321 tcggcacgga gcaggctctg ctcctctaca tctttcgcaa cgtcacgtcc aagagcctgt
    10381 cgctcagttt cagcgtcggc atgcgggtga cgctgatcat cgtcaacgca atcgtcggct
    10441 tcaccgcgat cctgctcatg accggaacct tgcgggtccg gcgaacggct caagccgatc
    10501 gcgccggcgg agagccggcg taggcgacaa gctcagacgg ccgctactga aagcgaccgt
    10561 ctgagaaacc ttctgagttc cagcctccgt tgctgttggt cacagccggc gagtggggac
    10621 tcttgggctg tgcccatgcg ttgccgacgc cgaacgtcac ggcgaccacg acaagagctc
    10681 ccaaggagaa catcttccgc agcatcctga tcacctcctc tcgttgcatt agaaccgcac
    10741 gtcctgcaag gcttctgccg cacgccggta gagcgccgcg gcggcactgt cgtcgccgcg
    10801 ccgtgatgca agatcgccct gggccgtcca ggcgaccgca cgatggcttg cggaggaaag
    10861 ctgtgacagg ctgagctcgg cctcggcgaa ggcgtgctcg gcttcgtcga gccgctgctg
    10921 ttccagcagg gcgcgaccga ggacgatctg ggcattgccg atctcgtcca ggaagtcagg
    10981 gcgcccagcg agcagctcga gggcatgccg ggattgctct tcggccaagc tccagtcgcc
    11041 ggcgccgagg tggacctgcg cgagcgagga gaccgcctgg gccgcgtcgg catcgctccc
    11101 gatttcgagg gcgacagaga atgcgcgctt gaggtacgag attgcgtcgt ccggcttgcc
    11161 gaggagatag ttcaggccgc cgaggttgtt gagcatgcgg ccgaggttct gcctgtcgcc
    11221 gtgctgctcg tacaggtcct ttgcccgctc ggcgaggctg cgcgcacgaa gccagtgccc
    11281 gtcgcgttcg gcaacgatcg acgcctggaa cgtgatgtgc gcgacggtga gggtgtcatc
    11341 gagtcgctcg gcgagttcga gcgcgcgctc gatgtcctcc cgggccgcct cgaaatcgcg
    11401 ctgacggcgc gagcaacggg aacgccactc gagaatatga gagcgcagcc ggtcgcaggg
    11461 atcacctgag cggtcggcga ggatgagcgc ttcgctcagc agcgtctgcg ctgtgttgat
    11521 cgacgtgagc ttgtagcggc agacacccat ccgatacagc acctcggcgc gctcgacatc
    11581 cgagaagacc gcctgctcgg ctagttcacg tgcgcgaacg aggagctcga gcgccgggcg
    11641 aagctctgcg cctgccattc gagcccaggc ttccgcgagc aaggcgcgga actcgagctc
    11701 acgagcctcc ggcgaaaact ccagtccggc cagcgcttcc agtgcttcgt cgtactgctg
    11761 tccctcgacg gcagcctcgg cacgcgtgat cgcggcctcg acctccgcat actcgtccca
    11821 ggactgaccg gtctccagat aggagacgtc gacgccgagg cgctcgccga tccaggaaag
    11881 tgtctctttc gtcggccgcg tcttcccgcg ctcgatctgg ctcatgtact ccttcgagaa
    11941 gcgctcgccg gccagctcgg actgcgtcag tcccgcggca acgcgaagct ggcgtaggcg
    12001 ggtgcctagg cccgaagtcg tgcgctgcgg cgcgcgaggg ggagtgagga tgcggccttc
    12061 catgttgcgg ctcttcacgt atcggcccgc tgccgcccca tgaggatcgc ttgcgagagt
    12121 gccccccatc gagggactag gggctttagg cgcggccggc tttgtgcagc tgcgcgtaga
    12181 tcgaacgagc cgtcttcgcc ggcacgcccg ggacgccttc gagctcctcc tgggacgccg
    12241 ccaggaagcg ctcagcggag ccgaagtgct ggagcagcgc ccgcctgcgc gccggtccga
    12301 cgccctccag ctcgtcgaag atcgaagcga aggagcgcgc gtctcgccgc tgccggtgga
    12361 agccgaccgc gaaacgatga gcctcgtcgc ggacccgctg caacaactgg agccccggcg
    12421 agtgcgcggc gagcacgatc ggatctgggc tgccgggaac gaagacctcc tcgacccgct
    12481 tggccaacgc gacgaccgcg acgcgcggga gatcgaacgc ctgcattgcc gccagcgcgg
    12541 ccgagagctg ccccttcccg ccgtcgatga cgaccagatt cggggtggct gcgaaagact
    12601 ggtcgtactc ctcggaggtg acatcggcga gccgcgagaa tctcctcgaa acgacctccg
    12661 cgatcgccgc gaagtcgtcc atcccgacct ggcccttgat tccgaacttc cgatagtgcg
    12721 ccttcttggc caccccgtcc tggaagacga ccatcgagcc gacgttgtcc tgtcccatta
    12781 ccgtcgagat gtcgaaacat tcgatccgga tcggcaacgc ctcgaggttg agcacctcgc
    12841 gcagctcctc gagggcctcc acccgccgca gccgcttcaa ctccgtctgc tcagcctccg
    12901 ctgcgagcgc atgacgggcg ttttccgtgg caagctcctg cagccgccgc ttctcaccgc
    12961 gttcggctgc gcgcacctcg acgcgggccc cccgtcgttc ggagagaaac tcggcgagcg
    13021 ccgatgtgtc cccggactcc cgcgggacga cgatctgcgg cgggacgctc ggcgcggagc
    13081 cgtagtactc caggaagaac gactcgagca cggttcccag gtcctgcccg ccgacgttct
    13141 cgaggtggaa cgagtagcgg tcgatcatct tcccgtccct cagcgggaag acctgcacca
    13201 cggcccggtc ggcctccagc gcaagcccga tcacgtcgat cgttccgacg gagcggagat
    13261 ccgccgcctg ccgttcggcg agatgctgga cgctccggag gcggttgcga tagcgcgcgg
    13321 cttcctcgaa acgctcgtct gccgcagccg cctgcatctt tcgttccagc tcacgctgga
    13381 tcggctcggt gtctccggac aggaactcga tcacctggtc gatgatcgcg cggtaatcct
    13441 ccttcgacac gtagccgacg catggcgcga gacaccgctc gatgtggtag tcgaggcagg
    13501 gaatgcccga gtggcgtccc ggcttcgggc cttcgcaggg ccggtacgga aagacgcggt
    13561 tcagcacgtc cagggtctcg cggaccttct tcgcgttcgc gtacggaccg aagtagacga
    13621 cgccgcggcg atggcgctcg cgcgtgaaca tgacccgggg gtattcgtcc gccaccgtga
    13681 cggcgatgta cgggaacgac ttgtcgtcac ggagccgaac gttgaagggc ggccggtgcc
    13741 gtttcacgag gttctgctcg aggtggagcg cctcgacctc gctctgcgtg acgatcacct
    13801 cgatgtcctc gacgcgctcg ggcagctgct ggatagcggt gcgcgtgtcg tggctctgct
    13861 gaaagtacga gcgaacgcga ggccgtaagg acttcgcctt gccgacgtag agaacctctc
    13921 cggcccggtc gcgaaagagg tagacgcccg gcttggccgg gagtcctttg agctggtttt
    13981 cgagccgcga cgccacgacg tccagcctag ccccggcaca aatgggcaag ccgcggccgg
    14041 caccggcaga tgaaagaagc ggcccgggtg gagagaccgg gccgcctgag aaaggattgg
    14101 tgaaggaagg tggcgagctt cccggaggcc ccctgcggcg agagccttgg caacacaatc
    14161 ctgactgttg tcaatatcgt tgggactcgc ggtgtccccg tcccccggac gagggatctt
    14221 cgctcctggc ggctccgggt tgacgccttc cggcgccatt cctacactgg cccgacccgg
    14281 cagcgtccta ccgcaaccgg ttttttccct tgctgaagcc ccgatccctc atcgccctgc
    14341 tctgtgcctt tgcggccctg gccgcgccga cccacgcttt gggcagtgag cggatgtaca
    14401 tgggcgcggc cgaggacgag ggccggaacg cagatccggc ggtggcgatg gcgaagatgg
    14461 agctcgccaa ggcggccggt ttcgacacga tccggatcac ggcggtctgg gagcccggtc
    14521 agagcgcggt tccggccggt cag
//
LOCUS       DASVEU010000115         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1435296,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000115 DASVEU010000000
VERSION     DASVEU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435296"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF225_04530"
     CDS             complement(<1..234)
                     /locus_tag="VF225_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7144553.1"
                     /translation="MAATSSGTATVTLPTDEQILITREFDAPRHLVYKAWTTPELVTR
                     WWSGDRGEMTIAEIDLRVGGVWRYVMVTDGGFEV"
     gene            complement(218..571)
                     /locus_tag="VF225_04535"
     CDS             complement(218..571)
                     /locus_tag="VF225_04535"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7144554.1"
                     /translation="MARAATTADAFNAVAEPRRRQILDALIGGERPVNDLVRLVGLAQ
                     PQVSKHLRVLREVGAVDVRHEGRQRLYRLNGRALKPIHDWVESYERSWSERFDQLDVV
                     LEELKLKEDEHGSDQ"
     gene            710..2992
                     /gene="lon"
                     /locus_tag="VF225_04540"
     CDS             710..2992
                     /gene="lon"
                     /locus_tag="VF225_04540"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7144555.1"
                     /translation="MSTLLLVPLDDNVVFPNMNVTISADVGDEDRVLLVPRHDGEYAK
                     VGTVAEVGDRLRLPGGIAAVSLVGLHRGVAGAAHTDAQGLLRVEVQERPDEEPPGVTT
                     RELEREYRAVVEEILELRGDDGRISSFVRSIREVGTLADTAAYAPEITFEQRIELLEA
                     VDVVERLELALRLQRERLAELQIRHRIREDVEEGAQRQQREYILRRQMESIRKELGED
                     DASVSEDYRGKIAEADLPDEVREQAEREVGRLERMGDQSGESSMIRTYLDWLLAVPWG
                     KRSEERLDPVNAREVLDNDHAGLDDVKERIVEYLAVRKLRQERGIAEDKRSGAILTLI
                     GPPGTGKTSVGESIARALNREFVRMSLGGVRDEAEIRGHRRTYIGALPGRLVRALRDA
                     GTMNPVILLDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHYLDVELDLSEVVFIAT
                     ANVAETIPGPLLDRMEVIRFDGYTVDEKVAIARGYLWPRQRERAGLREEEVSIDDITL
                     ELIVSQYTREAGVRQLERELGKLLRKTATTVASGTVEPPISIGEPTVRDALGKQRFYQ
                     ESAERTAVPGVATGLAVTGTGGDVLFVEATKMPGKDGLVLTGQLGDVMKESAQIALSY
                     VRSHAEKLGLDEHVFEDTSFHVHVPAGAIPKDGPSAGITMTTALASLLTGRPVKHTVG
                     MTGEVTLQGRVLPIGGLKQKALAAHAAGLTDVIIPERNRADLDDIPEDVRNEMSFHPV
                     MSLDEVLELSLESRSAHALS"
     gene            2979..3308
                     /locus_tag="VF225_04545"
     CDS             2979..3308
                     /locus_tag="VF225_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144556.1"
                     /translation="MRCRSCSPLPEEVRRAADLLERRWTVSILYVSHEGAVRFNEFLQ
                     ALGTIPPATLAQRLADLEQAGVLRREVIDARPPHVEYRLTQRGRKLRSLVNALARFAE
                     TGAGTAR"
     gene            complement(3316..3873)
                     /locus_tag="VF225_04550"
     CDS             complement(3316..3873)
                     /locus_tag="VF225_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7144557.1"
                     /translation="MSLTQYYTATTLDGFIADPDNSLDWLFTRKREEHGLLNYGDFIA
                     GVGAMAMGSTTYEWLLDHEFADKDRAEWKWPYDIPCWVFTHRQLPVVPGSGVEFASAD
                     IATVHKEMVAAAGNRNVWIVGGGDLAGQFADVGLLDEVIVSIAPVTLGQGAPLLPRRI
                     ELRLDELGRNGDFASAKFSVVRQTG"
     gene            3901..>4583
                     /locus_tag="VF225_04555"
     CDS             3901..>4583
                     /locus_tag="VF225_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX7144558.1"
                     /translation="MTKRTVGTREEWLAARNALLEREKELTRRNDDLARERRELPWLR
                     VEKEYSFETDDGPRTFGELFDGRSQLLVYNFMFGPDYTAGCPVCSSGADTYSGAVAHL
                     RARDVTFLCISRAPLEKLQAYRRRMGWNFPWVSSNGSDFNFDFGASHTKEELTPRLQG
                     NLGPIPGLAAECGTDPAGYLSEGPVFSAFALEDGVVYLTYSTTARGLEFMLGYYGFLD
                     RAPKGRDEG"
BASE COUNT          790 a         1525 c         1527 g          741 t
ORIGIN      
        1 gacttcgaat ccgccgtccg tcaccataac gtatcgccat acgccgccga cccgcaaatc
       61 gatctcggcg atcgtcatct cgccgcgatc tccgctccac caacgcgtga ccagctcggg
      121 cgtggtccac gccttgtaga caaggtgcct cggcgcgtcg aactcgcgcg tgatcaggat
      181 ctgctcgtcg gtggggagcg tcaccgtcgc cgtgccgcta ctggtcgctg ccatgttcgt
      241 cctccttcag tttgagttcc tccaacacga catcgagctg gtcgaaccgc tctgaccacg
      301 agcgctcgta gctctccacc cagtcgtgga tgggcttgag cgcgcggccg ttcagccggt
      361 acagccgctg ccgtccttcg tgccgcacgt caaccgcccc cacttctcgg aggacgcgga
      421 ggtgcttgga cacctgtggc tgggccagcc cgaccaggcg caccaggtcg ttcacggggc
      481 gctccccgcc gatgagggcg tccaggatct gccgtcgccg aggctcggcc accgcgttga
      541 acgcatccgc tgtcgtcgcc gctcgtgcca ttggctagac tatatgctca tataggaata
      601 tgtcaactgc gccctgcccg gaaggtgccc acccccgaac tccgctcggt aacttcatct
      661 gctgaagcta ctattttgga tgtagctacg acagaagggt gatgaagctt tgagcacttt
      721 gctgcttgtc ccactggacg acaacgtcgt cttcccgaac atgaacgtca cgatctccgc
      781 cgacgtgggc gacgaggacc gcgtgttgct cgtgccacga cacgacgggg aatacgcgaa
      841 ggtcggcacg gtcgcagagg tcggcgaccg cttgcgccta ccaggcggca tcgcagccgt
      901 cagtctcgtc ggtttgcacc gcggagtcgc cggcgccgcc cacaccgacg cccaggggct
      961 gctccgggtc gaggtgcagg agcgaccgga cgaggagccg cccggtgtaa cgacccgcga
     1021 gctcgagcgc gagtaccgcg cggtcgtcga ggaaattttg gagctgcgcg gtgacgacgg
     1081 tcgcatctcc tcgttcgtcc gctcgattcg cgaggtcggc acactcgcgg acacggcagc
     1141 ctacgccccc gagatcacct tcgagcagcg gatcgagctg ctcgaagcgg tcgacgtggt
     1201 cgagcggctc gagctcgcct tgcggctcca gcgggagcgg ctcgccgagc tgcagatccg
     1261 tcatcggatc cgcgaggacg tcgaggaggg cgctcagcgc cagcagcgcg agtacatcct
     1321 gcgccgccag atggagtcga tccgtaagga gctgggcgag gacgacgcct cggtttcgga
     1381 ggactaccgc ggaaagatcg ccgaggccga tctgcccgac gaggtccggg aacaagccga
     1441 gcgggaagtc ggtcggctgg agcggatggg cgaccagagc ggcgagtcct cgatgattcg
     1501 cacctacctg gactggctgc tcgccgtgcc ctgggggaag cgctccgagg agcggctcga
     1561 ccccgtcaac gcgcgcgagg tcctcgacaa cgaccatgcc ggactcgacg acgtcaaaga
     1621 gcggatcgtc gagtacctgg ccgtgcgcaa gctgcgacag gaacgcggca ttgcagagga
     1681 caagcgctcc ggcgcgatcc tgacgctgat cgggccgcca gggaccggca agacgtcggt
     1741 cggcgagtcg atcgcccgcg cgctcaatcg cgagttcgtg cgcatgtcgc tcggcggtgt
     1801 gcgcgacgaa gccgagatcc gcggtcaccg ccgcacctac atcggcgcat tgccgggacg
     1861 gctcgtgcga gcactgcgcg acgccggaac gatgaacccg gtgatcctgc tggacgaggt
     1921 cgacaaggtc ggcgccgatt ggcgcgggga tccctctgcc gcgctgctcg aggtgctgga
     1981 cccggcccag aaccactcgt tccgcgacca ttacctcgac gtcgaactcg atctttcgga
     2041 ggtcgtcttc atcgccaccg ccaacgtggc cgagacgatt cccggcccgc tgctcgaccg
     2101 tatggaagtg atccgtttcg acggttacac agtcgacgag aaggtcgcga tcgcacgcgg
     2161 ctatctctgg ccgcggcagc gtgagcgggc gggcctgcgc gaggaggagg tgtcgatcga
     2221 cgacatcacg ctcgagctga tcgtcagcca gtacacgcgg gaggccgggg ttcgccagct
     2281 cgagcgcgag ctgggcaagc tgttgcgcaa gacggcgaca accgtggcct ccggcaccgt
     2341 agagccgcca atttcaatcg gcgagccgac ggtgcgcgat gcgctcggca aacagcgctt
     2401 ctaccaggag tcggccgagc gaacggcggt gcccggcgta gcgaccggcc tggcggtcac
     2461 cggcaccggc ggcgacgtgc tgttcgtcga agccaccaag atgccgggca aggacgggct
     2521 cgtcctgacc ggccagctcg gcgacgtgat gaaggagtcg gcgcagatcg cgctctccta
     2581 cgtgcgctcg cacgcggaga agctcggcct cgacgaacac gtcttcgagg acaccagctt
     2641 ccacgtgcac gtccccgctg gagcgattcc aaaggacggg ccgagcgccg gaatcacgat
     2701 gacgaccgca ctcgcgtcgc tgctcacggg ccggccggtg aagcacacgg tcggcatgac
     2761 cggcgaggtc acgttgcagg gccgcgtgtt gccgatcggc ggcctgaagc agaaggccct
     2821 cgcggcccac gcagccggcc tgaccgacgt gatcattccg gagcgaaacc gggccgatct
     2881 cgacgacatc cccgaggacg ttcgcaacga gatgagcttc catccggtga tgagcctgga
     2941 cgaggtgctc gagctgtcgc tcgagtcgcg ctcggcgcat gcgctgtcgt agctgctcgc
     3001 ccctccccga ggaggtccgc cgcgcggcgg atctcctcga gaggcggtgg acggtctcga
     3061 tcctctacgt ctcgcacgaa ggtgccgtcc gcttcaacga gttcctgcag gcgctcggca
     3121 caattccgcc ggcgacgctc gcacaacggc tcgccgacct cgagcaggcc ggtgtgctcc
     3181 ggagagaggt gatcgacgct cgcccgcccc acgtcgaata ccgcctcacg cagcgtggtc
     3241 gtaagctgcg gtcactcgtg aacgcacttg cgcgcttcgc cgagacaggt gcaggcaccg
     3301 cccggtgacg ggtagtcagc cggtttggcg aacgacggag aacttggcgg aggcgaagtc
     3361 gccgttccgc cccagttcgt ccagtcgcaa ctcgatccgc cgtggcagca gaggcgcgcc
     3421 ctgaccgagc gtgacgggcg cgatcgacac gatgacctcg tcgagcagcc ccacgtcggc
     3481 gaactgaccg gctaggtctc ccccgccgac gatccagacg ttcctgttgc cggccgcggc
     3541 gaccatctcc ttgtgcacgg ttgcgatgtc cgcgctcgcg aactcgactc ccgagccggg
     3601 gacgaccggc aactgccggt gcgtgaagac ccagcacggg atgtcgtacg gccacttcca
     3661 ctccgcgcga tccttgtccg cgaactcgtg gtcgaggagc cattcgtacg tggtcgagcc
     3721 catcgccatc gcaccgacgc cggcgatgaa gtcgccgtag ttcaagagcc cgtgctcctc
     3781 gcgcttgcgg gtgaacagcc agtccagcga gttgtcgggg tcggcgatga agccgtctag
     3841 ggtggtcgcc gtgtagtact gagtgaggct cacgatgcac taccttaagg aggcgacgag
     3901 gtgacgaagc gcacggtcgg aacacgcgag gagtggctcg ctgcacgcaa cgcactgctc
     3961 gagcgagaga aagagctgac gcggcgcaac gacgacctcg cccgcgaacg ccgcgagctg
     4021 ccatggctgc gggtggagaa ggagtacagc ttcgagaccg atgacggtcc gaggacgttc
     4081 ggggagctct tcgacggacg ctcgcagcta cttgtctata acttcatgtt cggcccggat
     4141 tacacggccg gctgcccggt gtgctcatct ggggcggaca cctacagcgg cgctgtagcg
     4201 catctgcggg ctcgcgacgt gacgttcctg tgcatctcgc gggcaccgct cgagaagttg
     4261 caggcctacc ggcgccgcat gggttggaac ttcccctggg tttcgtcaaa cggaagcgat
     4321 ttcaacttcg acttcggagc ctcacacacc aaagaggagc tgacgccgag actgcaagga
     4381 aatcttggcc ccattccggg actggcggcc gagtgcggca ccgacccggc cgggtacctg
     4441 tcggaggggc cggtgttcag cgccttcgcg ctcgaggacg gggttgtcta cctcacgtac
     4501 tcgaccacgg cccggggtct cgaattcatg ctcggctact acggcttcct cgaccgggcg
     4561 cccaagggac gcgacgaggg cga
//
LOCUS       DASVEU010000116         7618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_852361,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000116 DASVEU010000000
VERSION     DASVEU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852361"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF225_04560"
     CDS             complement(<1..623)
                     /locus_tag="VF225_04560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7144559.1"
                     /translation="MARIVVAGSGSVGACIAYHLALLGARDVVLAERAEIASGSTGKA
                     MGGVRQQFSSAAEVRLARESIAFFEELGALWFQQVGYLFLATTADGLAELEERRKLQT
                     ELGVPVEQVDPRFVPGLAADDVLGAVFCATDGVADPPALTREVVRRAAELGVEVLERT
                     DARELERDVLVIAAGAYSGEFGVELPIRPLVRQLLETEPLEGLPDDLP"
     gene            complement(587..1444)
                     /locus_tag="VF225_04565"
     CDS             complement(587..1444)
                     /locus_tag="VF225_04565"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7144560.1"
                     /translation="MLRGALAAALTPLRDGGEALDEDAIAPYVEFLAAGGVDGLLALG
                     TTGEGFLLPLEQRLRAAQLFVEAASGRLLVAVHCGAQSTWDTVQLAAHAADIGADGVA
                     VMAPPYFVLDEAELLSHFDAAARACAPTPFYVYEFAARSGYPVPVAVLQRLREAAPNF
                     RGLKVSDKPWDRFAPYLIEGLDVFVGPETLLPQGLEAGAIGAVSALASTFPELVVTAV
                     RDRGGADLGPVRAALERFPFQAAAKVVVARRGVPIRPDVRRPLRVLTDEEWRELERWL
                     ESSSPVPVP"
     gene            1467..2249
                     /locus_tag="VF225_04570"
     CDS             1467..2249
                     /locus_tag="VF225_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7144561.1"
                     /translation="MAGLRIERDGHVLRVTLARPERRNAFDAALIGELTEAFADVGDA
                     RAVVLSGEGQSFCAGADVEWQRSAVDLSLAENVEDALRLYRMCETIDHCPAPVVARVH
                     GYALGGGSGLVACADVAIAAPGAVFGFSEVKLGIVPAVISPFVLPKIGAHARRYFLTG
                     ERFDAEAALRIGLIEEIADDLDAAIDRALSELLTAGPEALREAKRLIRERPAGEETAQ
                     IAARLRAGEEGQEGLRAFLEGRNARLPDGSEFGVRRPPRNSG"
     gene            complement(2260..3378)
                     /locus_tag="VF225_04575"
     CDS             complement(2260..3378)
                     /locus_tag="VF225_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7144562.1"
                     /translation="MAEIGVGMLGYAFMGKAHSNAFRKVEYMAGPPPLVPRLVAIAGR
                     NEDAVAEAARRYGYERWTTDWQDIVTDERIGLFDNGGPNSLHAEPTIAAAEAGKHVLC
                     EKPLARTADESHEVWRRVAATGVKHLCGFNYRFVPAVRLARELIDEGALGEIRHFRGR
                     YLQDWGDDPTLDTWRFDPDQAGSGALGDLGTHVIDLARYLVGEFSSVAGIVKTFVSGR
                     RVDDAIEAAVEFENGAIGTIESTRLALGRRNAFQWEINGSRGSLSFDMERMNELRVFL
                     EGEDRTRGFRTVLVTEANHPFMDFWWPPGHIIGWGDTFVHEVHHLLRSIADGSDVAPH
                     GATFEDGYRASEIGEAIVRSSAGGRREQLAFRGLTEGE"
     gene            3399..3647
                     /locus_tag="VF225_04580"
     CDS             3399..3647
                     /locus_tag="VF225_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144563.1"
                     /translation="MKTRYDDLIARLRAAAEPERTAPAELSGYLEKVRVHAYKVTDRD
                     VDELKAAGLSEDEIFEQTVSVAVAAGLERLDAGLGTLS"
     gene            3644..3865
                     /locus_tag="VF225_04585"
     CDS             3644..3865
                     /locus_tag="VF225_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144564.1"
                     /translation="MRLGVIDAGAGEVRARIGREPSGVLLTLYYRPDLFGRPFSDALD
                     LAMRGPSDWSAGERELFAAFVSSLNQCPF"
     gene            4013..4222
                     /locus_tag="VF225_04590"
     CDS             4013..4222
                     /locus_tag="VF225_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144565.1"
                     /translation="MTHADAEAVRAQGVSDEALIDAVHVAALFNMIVRLADSLGWDVP
                     PFDEFGARADAMLANGYALPPEDHE"
     gene            4219..4674
                     /locus_tag="VF225_04595"
     CDS             4219..4674
                     /locus_tag="VF225_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144566.1"
                     /translation="MTEAEARERAAAMLAQAGIELTPHEREQIEIADFGLGRLDEIGL
                     QIVVYVNTDRVCAKELVLFPHQHCPEHRHPPFEGEPGKEETFRVRRGAISLHVEGAGD
                     IVLGPGDQYTIPPNTLHSFEAGDEGAIVSEFSTTSRDALDVFTDPRIVR"
     gene            4691..6388
                     /locus_tag="VF225_04600"
     CDS             4691..6388
                     /locus_tag="VF225_04600"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7144567.1"
                     /translation="MSETPVDALRRRARTGLLESAERAPARAHFRAMGIDPARLGGPI
                     IGIASTWTGTMPCNLNQRELADRVSEGVSAAGGLALGFNTIAVSDNQSQGTPGMRASL
                     ISREVIADSIELMVHAHDFDGLVCVVGCDKTVPAALMALARVDKPAVLVYGGPMRAGH
                     WREREVTIQDVWEAVAAEERGLIPRGDLDELERFACPGAGTCAGHFTANTMAVAIDCL
                     GIACAGDALVPADEQELKGEAAARAGALAVQLVEQGMTARRFLDRRALLNAMAGIAAT
                     GGSSNGVLHLLAIAHEARVPLTLEELTGVSSRTPVIASLAPSGKWMAEDFHRVGGSRA
                     VIRELVRGGHIDGEAPTVDGGTLAAATADAPQPDGEVVYTLEKPFRPTGALHALRGNL
                     APEGSLVKVSGTTRRSHRGPARVFESEEACTDGVRAGLVADGDVLVVRYEGPAGGPGM
                     REMLSVTASVIGAGIGESVALVTDGRFSGATRGLMVGHVAPEAARGGPLAAVRDGDVI
                     AIDVDEGTLVLELDDTELAERLAGWEPPPPRYDVGVFARYRALVSSASDGAVLVPPA"
     gene            6394..7431
                     /locus_tag="VF225_04605"
     CDS             6394..7431
                     /locus_tag="VF225_04605"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7144568.1"
                     /translation="MQALVTQPGKTHTTHIEDVPAVKAREGEVLVRTLEVGVCGTDRE
                     ISEGLFGVAPENESLLVLGHEALAVVERDGYGFTRGQLVTATVRRSCGHCIACAEGSP
                     DSCLSGDYRERGITCLDGYDRELVAEDPAQLVEIPKSLGRLGVLAEPTSICERALRHS
                     RTIGSRQPWQLERALVIGAGAIGLLTTYLLRLADVAVWTASLEPENELVEQSGARYVS
                     THDTELSDLGKFDLVVEAAGNAQLMAQTLGLLGRSGVACLLGIDPRKQTVEIDGPVLG
                     VDTILENRVLFGSVNARREDWLAAVADLDRARERWPEALERFVTLRVPLDRFAEAFEH
                     RAGKATLVLSD"
     gene            complement(7433..>7618)
                     /locus_tag="VF225_04610"
     CDS             complement(7433..>7618)
                     /locus_tag="VF225_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7144569.1"
                     /translation="EKIREDAVERESFDPFFTFFLGGLAAFEGKTRVGAVGVSGLPGE
                     VDEALAQQALEAAGLSY"
BASE COUNT         1223 a         2606 c         2625 g         1164 t
ORIGIN      
        1 ggcaggtcgt cgggcaagcc ttcgagtggc tcggtctcca gcagctggcg tacgaggggc
       61 cggatcggca gctcgacacc gaactcgccc gagtaggcgc ccgcggcaat cacgagcacg
      121 tctcgctcga gctcgcgcgc atccgttcgc tcgagcacct ccactcccag ctcggccgca
      181 cggcgaacga cctctcgcgt gagtgccggt ggatcggcga cgccgtcggt ggcgcagaac
      241 accgcgccga gaacgtcgtc cgcagcgagt ccgggcacga agcgcggatc gacctgctcg
      301 accggcacac cgagctccgt ctgcagcttg cgtcgctctt cgagctcggc aagcccgtct
      361 gccgtcgtgg cgaggaacag atagccgacc tgctggaacc acaaggcacc aagctcctcg
      421 aagaaggcga tcgactcccg ggcgagccgc acctcggccg cagaggagaa ctgctgtcgc
      481 actccgccca tcgccttgcc ggtcgaaccg gacgcgatct ctgcgcgctc tgcgagcacc
      541 acgtcacgcg cgccgagcag cgcaaggtgg tacgcgatgc aggctcctac ggaaccggaa
      601 ccggcgacga cgattcgagc catcgctcga gctccctcca ctcctcgtct gtgagcacgc
      661 gcagcgggcg gcgcacatcg ggccggatcg gtacgccgcg tcttgccacc acgaccttcg
      721 cggccgcctg gaatgggaaa cgttcgaggg ccgcgcggac aggcccaagg tcggcgccgc
      781 cccgatcccg cacggctgtc acgacgagct ccggaaatgt cgacgcgagc gccgacaccg
      841 cgccgattgc gcctgcctca agtccctgcg gcagcagcgt ctccgggccg acgaagacgt
      901 cgaggccctc gatcaggtag ggagcaaagc gatcccacgg tttgtcggac accttcagcc
      961 cgcggaagtt cggcgccgcc tcgcgcaacc gttgcaacac ggcgacggga accggatagc
     1021 cactcctggc ggcgaactcg tagacgtaga acggcgtcgg cgcgcaagcc cgcgctgcgg
     1081 cgtcgaagtg tgaaagcagc tctgcctcgt cgagcacgaa gtacggcggc gccatcacgg
     1141 cgacgccgtc ggccccaatg tctgcggcgt gagcagcgag ctggacggtg tcccaggtcg
     1201 attgcgcacc gcagtgcacc gccacgagca gtcgtccgct cgcggcctcc acgaagagct
     1261 gcgccgcgcg tagacgctgc tccaacggca ggagaaagcc ctcgccggtc gtcccaaggg
     1321 cgagcaggcc gtccacccca cccgcggcga ggaattccac atacggcgcg atcgcgtcct
     1381 cgtcgagggc ctcgccgccg tccctgagcg gcgtcagcgc cgccgccagt gctcctcgca
     1441 gcatggacca actctaagct gaagccgtgg ccggactgag gatcgaacgt gacggacacg
     1501 tgttgcgggt cacgctcgcg cgtcccgagc gccgaaacgc cttcgatgcg gcgctgatcg
     1561 gggagctcac agaagcgttt gcggacgtcg gcgacgcgcg cgccgtcgtc ctctccggcg
     1621 agggccagag cttctgcgcc ggcgcggacg tcgagtggca gcgttcggcg gtcgatctct
     1681 cgctcgccga gaacgtcgag gacgcgctga ggctctaccg gatgtgcgag acgatcgacc
     1741 actgtccggc accggtcgtc gcccgcgtcc acggctacgc gctcggcggc ggttccggcc
     1801 tggtcgcgtg tgcggacgtt gcgatcgccg cgcccggtgc ggtgttcggg ttctcggagg
     1861 tcaagctcgg aatcgtcccc gccgtgatct cgccgttcgt gctcccgaag atcggagcac
     1921 acgcgagacg ctactttctc accggcgaac gcttcgacgc ggaggccgcc ctacgaatcg
     1981 gcctgatcga ggagatcgcc gacgatctcg atgccgccat cgaccgcgcg ctctccgagc
     2041 tgctgacagc agggcctgag gcgctccggg aagcgaagcg tctgattcgc gagcggccgg
     2101 cgggcgagga gacggcccag atcgccgcac gactgcgtgc gggcgaggaa ggacaggaag
     2161 gattgcgagc gtttctcgaa ggacggaacg cacgactgcc tgacggcagc gagttcgggg
     2221 ttcgtcgccc gcctcgtaac tctggctagt ggcgcctcgt cattcgcctt cggtcagacc
     2281 ccggaacgcc agctgctcgc gccgaccgcc ggcgctcgat cgcacgatcg cctcgccgat
     2341 ctccgacgcg cgatagccgt cctcgaaggt cgcgccgtgc ggtgcgacgt cgctgccgtc
     2401 ggcgatcgaa cggaggaggt gatgcacctc gtgcacgaag gtatctcccc agccgatgat
     2461 gtgcccaggc ggccaccaga aatccatgaa cggatggttt gcctctgtca cgagcaccgt
     2521 cctgaagccg cgtgtacgat cctcgccttc gagaaagacc cgcaactcgt tcatgcgctc
     2581 catgtcgaag gagagcgagc cgcgggagcc gttgatctcc cactgaaacg cgttgcgccg
     2641 tccgagcgcg aggcgtgtcg actcgatcgt cccgatcgcg ccgttctcga actcgacagc
     2701 cgcctcgatc gcgtcgtcca ccctgcgacc gctcacgaat gtcttcacga tgccggcgac
     2761 cgaactgaac tcgccgacga ggtagcgcgc gaggtcgatc acatgcgtac cgagatcgcc
     2821 gagcgcgccc gatcctgcct ggtccgggtc gaagcgccac gtgtcgagcg tcgggtcgtc
     2881 gccccagtcc tgcaagtaac ggccgcggaa gtggcggatc tctccgagtg cgccctcgtc
     2941 gatcaactcg cgcgcgagcc gcaccgcggg gacgaagcgg tagttgaacc cgcacaggtg
     3001 cttgacgccg gtcgctgcga cgcggcgcca cacctcgtgg ctctcgtcgg ccgtgcgcgc
     3061 gagcggcttc tcgcagagga cgtgcttgcc cgcttcggcg gcggcgatcg tcggctcggc
     3121 atgcagcgag ttcgggccgc cgttgtcgaa gagcccgatc cgctcgtccg tgacgatgtc
     3181 ctgccagtct gtcgtccagc gctcatagcc gtaccggcgc gccgcctcgg ccaccgcatc
     3241 ctcgtttcgc ccggcgatcg cgacgagccg tggcacgagc gggggcggcc ccgccatgta
     3301 ctcgaccttg cggaaggcgt tcgagtgcgc cttccccatg aacgcgtagc ccaacatgcc
     3361 gacgccgatc tccgccattg cgcgatagta cgcagatcgt gaaaacgcgg tacgacgacc
     3421 tgattgcgcg gctccgcgct gcggccgagc cggagcgaac cgctccggcg gagctgagcg
     3481 gttacctcga gaaggtgcgc gtgcacgcgt acaaggtcac cgatcgcgac gtcgacgagc
     3541 tgaaggctgc aggcctcagc gaggacgaga tcttcgagca gaccgtctcc gtggctgtgg
     3601 ctgccggcct cgaacgactc gacgcgggac tggggacgct ctcgtgaggc tgggcgtcat
     3661 cgatgcgggc gcgggcgagg tgcgcgcacg catcggccgc gagccaagcg gggtcttgct
     3721 gacgctctac taccggcccg acctcttcgg gcgccccttc tccgacgccc tcgatctcgc
     3781 gatgcgcggc ccgtcggact ggtccgccgg cgagcgcgag ctcttcgctg cgttcgtctc
     3841 ttccctcaac cagtgtccct tctgaacgaa cgcccacggc gcggtcgcgt cgtatgcaat
     3901 cggcagggag gtctgggagg cggtcgagcg tgactggcgc accgctccga tcgctgaagg
     3961 cctccgcgcc acactcgggt tcatcgagaa gctgacgcta cggccgcagg agttgacgca
     4021 tgcggatgcc gaagcggtcc gcgcgcaggg tgtcagcgac gaagcgctga tcgacgctgt
     4081 gcatgtcgcc gccctgttca acatgatcgt gcgtcttgcc gactcactgg gctgggacgt
     4141 cccgccgttc gacgagttcg gcgcccgtgc agacgcgatg ctcgccaacg gctacgcgct
     4201 tcctccggaa gaccacgagt gaccgaggcc gaggcgcgcg aacgggcggc ggcaatgctt
     4261 gcgcaggcag gaatagaact gacaccccat gagcgcgagc aaatcgagat cgcggacttc
     4321 ggtctgggcc ggctcgatga gatcggactg cagatcgtcg tctatgtgaa caccgatcgc
     4381 gtgtgcgcga aggagctcgt gctgttcccg caccagcatt gccccgagca ccggcacccg
     4441 ccgttcgagg gcgagccggg aaaggaggag acgttccgcg tccgccgcgg tgccatcagc
     4501 ctgcacgtcg agggtgcagg cgacatcgtg ctcgggccgg gcgaccaata cacgatcccg
     4561 ccgaacacgc tgcactcgtt cgaggcgggc gacgagggcg ccatcgtctc cgagttttcg
     4621 accacgagcc gcgacgccct cgacgtcttc acggaccccc ggatcgtgcg ttagcagtac
     4681 agtcggcgac gtgagtgaaa cgccggtcga cgccctccga cgacgggccc gcaccggctt
     4741 gctcgagagc gccgaacgtg ccccggcgcg cgcgcacttc agggcgatgg gcatcgaccc
     4801 ggcgcgattg ggcggaccga tcatcggcat cgcgtcgacg tggacgggaa cgatgccgtg
     4861 caacctcaac cagcgcgagc tcgccgatcg agtgagcgag ggcgtgtccg cggcgggcgg
     4921 ccttgcgctc ggcttcaaca cgatcgccgt ctccgacaac cagtcgcagg gaacgcccgg
     4981 catgcgggcg tcgctgattt cgcgcgaggt gatcgccgat tcgatcgagc tgatggttca
     5041 cgcgcacgat ttcgacgggc tcgtgtgtgt agtcggttgc gacaagacgg tgccggccgc
     5101 actgatggcg ttagcgcgcg tggacaagcc cgccgtgctc gtctacgggg gtccgatgcg
     5161 tgcgggccac tggcgcgaac gcgaggtgac gatccaggac gtatgggaag cagtggcggc
     5221 tgaggaacgc gggctcatac cgcgcggaga cctcgacgag ctcgagcgct tcgcttgccc
     5281 gggcgcgggc acctgtgcag gacacttcac cgcgaacacg atggccgtcg cgatcgactg
     5341 cctgggaatc gcctgcgccg gcgatgcgct cgtgccggcg gacgagcagg agttgaaggg
     5401 cgaggccgct gcgcgagccg gcgcgctcgc cgtgcagctc gtcgagcaag gcatgacggc
     5461 acgccgcttc ctcgaccggc gcgcgctact gaacgcgatg gccggtatcg ccgcaacggg
     5521 cggctcgtcg aacggcgtgc ttcacctgct cgcgattgcg cacgaagcga gggtgccgct
     5581 gacgctcgag gagctgacag gcgtctcgag tcgcacgccc gtgatcgcca gcctcgcgcc
     5641 ctcaggaaag tggatggcgg aggacttcca ccgcgtgggt ggttcgcgcg ccgtgatccg
     5701 cgagctggta cgcggcgggc acatcgacgg cgaggcgccg accgtcgacg gagggacact
     5761 cgcggccgcg accgcagacg cgccacagcc ggatggcgaa gtcgtctaca cgctggagaa
     5821 gccgttcagg ccgacgggcg cattgcatgc gctccgtggc aacctcgcgc ccgagggtag
     5881 cctcgtcaaa gtttccggaa cgacgcgccg ctcgcatcgc ggccctgcgc gggtgttcga
     5941 aagcgaggag gcgtgcaccg acggcgtgcg cgcgggcctc gtcgcggacg gcgacgtgct
     6001 cgtcgtgcgt tacgaaggcc ccgcgggcgg tccaggtatg cgcgagatgc tgagcgtcac
     6061 ggcgtccgtg atcggtgccg ggatcggcga gtcagtcgca ctcgtcaccg acggccgctt
     6121 ttccggcgcg acccgaggct tgatggtggg gcatgtcgca cccgaggccg cacgcggcgg
     6181 accgcttgca gctgtgcgag acggcgatgt gatcgccatc gacgtggatg agggcacgct
     6241 cgtcctggag ctcgacgaca ccgagctggc agagcgcctc gccggctggg agccccctcc
     6301 gccgcgctat gacgtcggtg tgttcgcgcg ctaccgggca ctcgtgtcct ctgcgtctga
     6361 cggcgccgta ctcgtcccac ccgcgtaacg acggtgcagg cgctcgtcac gcaaccgggt
     6421 aagacgcaca caacgcacat cgaggacgtt ccagctgtga aggcgcgcga gggcgaagtg
     6481 ctcgtccgca cgctcgaggt gggcgtgtgc ggtaccgacc gcgagatctc ggaaggcctg
     6541 ttcggggtag cacctgagaa cgaatcgctg ctcgtgctcg gccacgaggc gctggcggtc
     6601 gtcgagcgag acggctacgg cttcacgcgc ggccagcttg tcaccgcgac cgtccgccgc
     6661 tcatgcggcc attgcatcgc gtgcgcggag ggctcaccgg attcgtgtct cagtggcgac
     6721 taccgcgagc gcgggatcac gtgcctggac ggatacgacc gcgagctcgt cgcggaggat
     6781 ccggcgcaat tggtcgagat tccgaagtca ctcggtcgcc tcggcgtgct cgcggagccg
     6841 acctccatct gcgagcgcgc gctccgacat tccaggacga tcggtagccg gcagccctgg
     6901 cagctcgagc gcgcgctggt catcggtgca ggtgcgatcg gcctgctgac gacgtatctc
     6961 ctgcgtcttg cggacgtggc agtgtggacg gcatcgctcg agcccgagaa cgagcttgtc
     7021 gagcagtccg gcgcgcgcta cgtctccacg cacgacacgg agctgtcgga cctcgggaag
     7081 ttcgacctcg tggtcgaagc ggccggcaat gcacagctga tggcgcagac gctgggcctg
     7141 cttggccgga gcggagtcgc gtgcctgctc gggatcgacc cgcgcaagca gaccgtcgag
     7201 atcgacggcc cggtgctggg cgtggacacg atcctcgaga accgcgtgct gttcgggagt
     7261 gtcaacgcgc gccgcgagga ttggctggcc gcagtcgccg atctcgaccg agcgcgcgag
     7321 cgctggccgg aagcgctgga gcgatttgtc acgctccgcg tcccgctcga ccgcttcgct
     7381 gaggcgttcg agcatcgcgc cggcaaggcg acgctcgttc tatctgacta gatcagtagg
     7441 aaagacctgc ggcctcgagc gcctgttgcg cgagcgcctc gtcgacctcg cccggcaagc
     7501 cgctcacgcc gacggcaccc acccgtgtct tgccctcgaa cgcggcgagc ccgccgagga
     7561 agaacgtgaa gaacggatcg aagctctcgc gctcgacagc gtcttcgcgg atcttctc
//
LOCUS       DASVEU010000117        28806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_660046,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000117 DASVEU010000000
VERSION     DASVEU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 28806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28806
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660046"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF225_04615"
     CDS             complement(<1..659)
                     /locus_tag="VF225_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX7144570.1"
                     /translation="MGPVGRWVVIAILAAFVLNYWLASRSLSEPPRVQIPYSPLFLNE
                     VRQGNVDRIISKGTSVQGLFKSPVRYPPTGSGSRSSERFSTLIPSFADTAALSKLLEE
                     NNVVINAEPLESSAAWWETLLYGFGPTLLLIGLLVLLMRRGGRSALGSFGRSRAKRYE
                     ASAERVTFDDVAGIEEAKDELTEIVDFLKNPDRYGRLGGRIPRGVLLSGPPGTGKTLL
                     AR"
     gene            779..1411
                     /locus_tag="VF225_04620"
     CDS             779..1411
                     /locus_tag="VF225_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX7144571.1"
                     /translation="MGVSVKLFAGLRERAGWSERELDGVDRVADVWPALDLGDEPEGL
                     LYAVNKEYADRDRPLADGDEVALIPPVSGGAFRLTAEPLSVDAVVDEVRTDQAGAIAT
                     FIGTTRLQSRGRTVLHLDYEAYEEMAERVMAQIAAELKERYDLCQIAIHHRTGRLEIG
                     EASVVIAVSAPHRQDALAACKDAIDTLKEQVPLWKKEFYEGGEEWIGRGS"
     gene            1514..1744
                     /locus_tag="VF225_04625"
     CDS             1514..1744
                     /locus_tag="VF225_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144572.1"
                     /translation="MEDIDQSRFALMTALGEAHKARPDEYPNPGTLVAMSDEGVRAYA
                     VGLLRLLEAAPGGDGLETELQKELRLILGDTP"
     gene            1846..2646
                     /locus_tag="VF225_04630"
     CDS             1846..2646
                     /locus_tag="VF225_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7144573.1"
                     /translation="MEQYPQEWRQYEPIQPKGFDWRGLFRRIWAPIAAIGAFLAKFGV
                     VLLKFKFLFSMFVSAAVYVWIGGWWFGIGLVVLLFVHEMGHVLEAKRQGLPVSAPLFI
                     PFMGALITLKEMPHNAWREAKLAIGGPLLGSAGALAIYIAGVAYDSNSLKALAFLGFF
                     INLFNLLPVVPLDGGRITAALHPALWLVGFLGLLGLVIWKPNPILIIILLFSASELWR
                     RWKMRHHPEMQEYYRVKPHQRLIIGVLYFGLAIALVLGMHATHVPRNF"
     gene            2646..3350
                     /locus_tag="VF225_04635"
     CDS             2646..3350
                     /locus_tag="VF225_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7144574.1"
                     /translation="MEDRRILSAEEKTEDLEQDIALIAHEFREGFEAVDMIVQPAVTI
                     FGSARITEDNPVYAEARTAGRRFAELGWAVVTGGGPGVMEAANRGAKEGGGLSVGFNI
                     ELAHEQAPNPYLDISLTFRHFYARKTMFVKAAEGFVVFPGGFGTVDELFEALTLIQTG
                     KVFHFPVVLYGSDYWGELIDWIKGELLADGMISPEDLDLLFVTDDLDEVVERVRAGYE
                     SRSSATPAAPVKADAQ"
     gene            3382..4656
                     /gene="selA"
                     /locus_tag="VF225_04640"
     CDS             3382..4656
                     /gene="selA"
                     /locus_tag="VF225_04640"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7144575.1"
                     /translation="MRLRDLPSVDELARQSADPLAVEAARKVVARAREQIQRGDDPGD
                     LAALLQNELDAAHTSHLRRVINATGVIVHTNLGRAPLAEAALDRVREIARGYSNLEYE
                     LAAGARGSRQEHVADSLRRLTGAEAALVVNNNAAAVMLALAALAEGREVLVSRGELIE
                     IGDGFRIPDVLERSGARLREVGTTNRTRASDYEAAIGTETAVLLRVHQSNFRVVGFTA
                     QPSLGELADIARRHDLPLVDDLGSGALIDIGDEPTARASLAAGADLVCFSGDKLLGGP
                     QAGIVLGRAELVEKLRRHPLQRALRADKLTLAALEGTLALAVDAPDEIPVLRMLREPV
                     EAVRVRAARLAELVHGEVEETVARAGGGSLPLTELPSFACAVEEELAVKLRAGDPPVI
                     AVVRDGRTLLDCRTLTDAEADEVAVAVEAARA"
     gene            4653..5615
                     /locus_tag="VF225_04645"
     CDS             4653..5615
                     /locus_tag="VF225_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7144576.1"
                     /translation="MNDVPPEARLWNLIRGALGTKALGIAADLGVADALATGPRSVAE
                     LAEENGADTDTLHRILRALASDGVFAEDEPGAFRNTEASELLRSDNDDRWREFAHLFG
                     EVCYPAIGALDVRLTDVPFARAYGTDIWSWFATQPEERSNFDRAMEGGQAERADKLAA
                     LEWREGEAVVDVGGGTGRLLRDLLERRPELRGIVFDLPEADREETAVGDRITFVSGSF
                     FEQVPEGDAYVLSKILHDWDDERAAAVLRTVRAAAPDEARLLVLDSVVPPGNDQAGVK
                     WLDLLMLVLQRGRERTESDWRALLDGAGFRVEQIEDGLIQARCR"
     gene            5606..7213
                     /gene="selB"
                     /locus_tag="VF225_04650"
     CDS             5606..7213
                     /gene="selB"
                     /locus_tag="VF225_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX7144577.1"
                     /translation="MPLTVGTAGHIDHGKTWLVRALTGKDTDRLPEEQARGISIDLGY
                     APLELPDGRRLSLVDVPGHERFVRTMVAGATGIDLFLLVIDAGEGARPQTREHLAILR
                     LLSVDRGVVAVTKADAVEPDALELALDEARELVPGSEVVAVSAKTGSGLDELRAALAR
                     ASVDTERADAPTRLYVDRVFSLHGIGTVATGTLWSGRIGPGAILRAEPGAREVRVRSV
                     QVHDREVDVAEAGQRVAVALPGVERSDLGRGDALVERDAYDVSFRLDVEVEELAPIPP
                     RVLVHHGTATVLARVVRVDRFAQLRLDAPIVAARGDRVVLRAGTTVGGGLVIDAAPPR
                     HADATRFERSARGEVLLHAPVLDADGGWSFSDEWLEELRVELERRLDAADPLDPGVPA
                     PTQAWAPAVLPRLGLERRGAKLYRPGRAGSLGEREREAAELEARLGLEPVKVEDAGLA
                     RFLEQQGRLVRTGDGYAVSAAAYERARDVLFEELGASGRITLARFRDLLGVSRKTAQL
                     LLERFDADRVTRRDGDARVLRRSAPRP"
     gene            complement(7370..8269)
                     /locus_tag="VF225_04655"
     CDS             complement(7370..8269)
                     /locus_tag="VF225_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7144578.1"
                     /translation="MDGVVKVACVQAEPVAFDRAATIDKVAGLAGEVAGAGARLALFP
                     ETFIPVYPSNRWVRYLAGWGGPEAGAAREVFARLMQQSVTVPGADSDRLAEIARANDL
                     WLAVGVNELDGGTIYNSLLVYSPDGGLALHHRKLMPTNHERLVWGLGDGRGLETIPTD
                     LGKVGGLICWENLMPLARFALYQGGVEVYLAPTADDSEDWHDSMKHIARESRAFVLSC
                     CVFQRASAYPDDVPLADGDELVGAGGSAILAPDGTYLAGPLWNEEGILYADLDPQRLY
                     EARQRFDPAGHYNRPDVLRLQIR"
     gene            complement(8279..9289)
                     /locus_tag="VF225_04660"
     CDS             complement(8279..9289)
                     /locus_tag="VF225_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7144579.1"
                     /translation="MVGRAFILGGTGNVGTAIAHSLAADGWQVTVAGRTERAAPVGIE
                     QVALDRDEQGALSVADGFDVVVDVIPFEAAHAEQLLALETGALVAISSASVYADEQGR
                     TLDEAQGVDDFPEFPLPIPETQPTVAPGDDTYSTKKVKLERILLENGHVPAAIVRPCA
                     IYGPGDRMGREWHFVKRALDRRPFVVLTNRGAGQFHTTASENIGELVRLIAARPRTGV
                     FNCGDPDPPTVLEIARAIGEAAGHRFEEVLLEEPAARGEVGQTPWSAAKPMLVDMAKA
                     EQELGYHAATTWAEALPRQVRWLIGATRDRDWREVLTRGADYLKFDYEDEDELVADLP
                     KA"
     gene            complement(9296..9745)
                     /locus_tag="VF225_04665"
     CDS             complement(9296..9745)
                     /locus_tag="VF225_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7144580.1"
                     /translation="MSGHVEAIFVASERGELPAPVERVRAYAGRGLQGNRYFWEDGDA
                     PPGRGVTLIAAEAMDAVALEGNVSIEPAATRRNVLTRGIDVNELVGKRFRIGDVECEG
                     VELCEPCAHLESMTQPGLIKALVHRGGLNADILSDGEISIGDPVVAV"
     gene            complement(9756..11225)
                     /locus_tag="VF225_04670"
     CDS             complement(9756..11225)
                     /locus_tag="VF225_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7144581.1"
                     /translation="MTIELQIIGSLLRAVAEEMGAVLVRSAFSANIKERRDCSTALFD
                     ERGRMVAQAEHIPVHLGAMPDAVAAVMRQDPAFGEVWALNDPYAGGTHLPDITLVSRT
                     ETGFAVTRAHHADVGGREPGSMPADSRTLDEEGVVIPPTRLDDDVIAGLVARMRNPEE
                     RQGDLRAQLAAHRLAEQRVAELCERRGRDTVIAAMDELFAYSERRMRAGIAALPDGRF
                     AAHDVLEAIAGDLEVHATVTVEGETVAIDFTGTSPQHAGNLNCPLSVARSACFYVVRL
                     LVDPALPGSGGAFAPVNVTAPAGCLVNARPPAAVAAGNVETSSRIVDVVMRAFGEAIE
                     VPAQGQGTMNNVTLGNERFTYYETIGGGQGACPDADGPSGVHVAMSNTLSTPVEALEL
                     QYPLRVERYGLRLGSGGAGRHRGGDGVLRELRVLEACRLSIISERRVHRPQGERGGDP
                     GAAGRNSLNGEPLPAKVTRDLTAGDVVTIETPGGGGHGG"
     gene            complement(11222..12958)
                     /locus_tag="VF225_04675"
     CDS             complement(11222..12958)
                     /locus_tag="VF225_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7144582.1"
                     /translation="MAVLGVDVGGTFTDAVVLDGGRVTTAKVLTAPHQEESVLAAARA
                     AGADRVERFAHGTTVATNALLERKGARTAFVTTAGFEHLLHLRRQTRAQLYRPCAAWP
                     EPLVPLDRSYGIRGRMGPEGELEPLELGTLPDIDADAVAVCLLHAYRDPSHERVVVEE
                     LRRRLPDAHVVASHEVAPEFREYERASTTAADAYLGPVVARYLHALADAAAAAGLPEP
                     LVMRSSGGVATPDEAADHPATILVSGPAAGVVGAARLAALDGIENAIAFDMGGTSTDV
                     CLIAGGRAQRASERTVAGLPIRLSTVDLHTVGAGGGSLVWTDAGGALHVGPQSAGADP
                     GPACYGRGGTQGTVTDANLLLGRLPHQLAGGVELDRAAAERALAGIDPAAVVDVVNAE
                     MLRALRVVSVERGHDPADFALVAFGGAGPLHACELADELGIRTALVPEAAGVLSALGL
                     VASEERRDAVRSYLVPLRDAGELPREGEADLRYAGQSFELTVPLQEDLADAFHRAHEE
                     QYGFAERDREVQLVAVRTADIRRGPELELPRGKETTIYGPAVVELAGSTCWIPPGWVG
                     ARDGRMLRLTRQ"
     gene            12976..13647
                     /locus_tag="VF225_04680"
     CDS             12976..13647
                     /locus_tag="VF225_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7144583.1"
                     /translation="MAIRGLLFDFDGLLVDTETPSRLVWEELYREHGHELPQDRWATL
                     IGTIGAPFDPLGHLEELVGRRLDEQALTTRRRAREYELTDLEELRPGVEEYFAEAERL
                     GLRTAVVSSSDDDWIARHLGRLGHLEGLDTIVAANGDTARAKPRPDLYLEALDRLELE
                     PHEAIAFEDSPNGVTAAKAAGLVCVAVPNPMTATLALDDADLVLESLADVPLRGLLER
                     FRSAR"
     gene            13644..13988
                     /locus_tag="VF225_04685"
     CDS             13644..13988
                     /locus_tag="VF225_04685"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7144584.1"
                     /translation="MKALTQMKCVACRKDAPTLADAEIAELHPQIPDWEIVELDGIKR
                     LRRVFAVDDFAQALELTNEVAELAEQEQHHPALLTEWGRTTVTWWTHKIKGLHRNDFI
                     MAAKTDQLYLRY"
     gene            complement(13985..14890)
                     /locus_tag="VF225_04690"
     CDS             complement(13985..14890)
                     /locus_tag="VF225_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7144585.1"
                     /translation="MILCLVDGDPVSLEALTFARELGDVEALIFEETDVDGYTARARR
                     VVHPRLGDYAPEAWAQAIVELEPEVVVAPGTDRGHEVLAHVAARLGVPLAANVVAAAT
                     DGDALQLTRQRWGGSLLEEARLTGTPKLLTVAAHAVVAQPAGVTTTTEEFTPSLADEH
                     FVVRVSGREQPTAGTVRLDTARVVVGGGRGVGSKEGFELLEELANLLGGAVGGSRVVT
                     NLGWRPHSDQVGQTGARIAPDLYIACGISGAIQHIVGCKAAKRILVINKDQSAPIVAR
                     ADWAVIGDLHEIVPAIVAELRKNGV"
     gene            complement(14887..15648)
                     /locus_tag="VF225_04695"
     CDS             complement(14887..15648)
                     /locus_tag="VF225_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7144586.1"
                     /translation="MNVIVCVKRVPETGARITLTGDAQAIDTSLLGFTISPHEECAVE
                     EALRIVGKHGGSTTVLTLGPPDAAEQLQNAMAMGVDNAVLLETDGSDWNAEATAAALA
                     DAISAQPELFDVLLFGNEAADTGDYQVGVRVAHALGLPCVTGIKGIAIEDRKLTARRE
                     AGGGWEIFELPLPAVVTVKEGINLPRYASLPGRLRAKKTPIARIEPQPRASALETIRL
                     VVPPEQETHAEILAGAAAAPRVVEVLRELGLVVTS"
     gene            complement(15645..18128)
                     /locus_tag="VF225_04700"
     CDS             complement(15645..18128)
                     /locus_tag="VF225_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7144587.1"
                     /translation="MVGAGIVGNSIAYHLSRLGWTDLVLLEQGPLPNPGGSTGHASNF
                     IFPVDHSKEMTTLTKESARQYIELGLATISGGVEVARTKERMEELNRRMSSALSWGEE
                     GCELLTPAQVLKKVPFLNTDVILGGFYTPGVSVVDPLRAGTLMREYAQERGASVFAST
                     EVTGIDVENNRVRRVHTDKGSIDAEYVFVACGIWSPRIARMAGATIPLTPAVHQMISV
                     GPIPLFEETPGEISFPIVRDVDTNMYERQNGSDMEVGSYAHRAILLEPDQIPSIEESA
                     LSPTELPFTSEDFDPQLEQALELFPEILTVEGAGIRHAINGLLSLTPDGMPLLGETEV
                     KNLWSVAAIWIKEAPGIAKAVAEWAVEGAPETDLHTADVARFYPHQRTKRHVDARSSE
                     GYNRTYGIVHPMEQWLSNREVRLSPFYARERELSAVFYETAGWERPWWYASNEPLLEE
                     YGDRVMPREAEWESRWWSPIINAEHLAMRDRAAMIDISAFAVFDIVGSGALEHLQRLA
                     LAEMDVAVGRVVYTSILDQHGGFKADLTIMRLGDNFFRVVTGGLDGPRDRKWFEDQLP
                     ADGSAALDDVTSAWCTLGLWGPRARDILEATTSADVSHEGFPFLRCRWIDIGPVRTLA
                     SRISYVGELGWELYAPMEQGAALWDTIREAGQPFGVVPSGIGVYGTTGRIEKGYRLYG
                     AELETEYNVVEADMQRPKVKDADFIGKDAHVRHRNEEPAAILSTLTVDDNTSPSNGKK
                     RYMLGREPILTADGARIADSKGRGSYVTSAGSGPSVGKHVLLAYLPPEHAEEGNKLLV
                     QYMGDRYPVSVAVKGSRPLFDPENTRVRS"
     gene            complement(18164..18850)
                     /locus_tag="VF225_04705"
     CDS             complement(18164..18850)
                     /locus_tag="VF225_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7144588.1"
                     /translation="MAEAKGQVSVLQASSGATASLADRAYHAIREMIVSVELRPGAVI
                     DERGLMDRLGIGRTPTREALRRLSQERLVEVYPRRGMFVTSVEIRDLASLAEVRSALE
                     SHVARLAAERATDEDRNALETLLGELGGQSELDARELMALDERIHRQVYSCAHNPFLE
                     ATLEEYYVLALRIWYLALDRAPELEEAVLGHRELLEAIRDGDADRAEATMRRHVLDFE
                     EVMRRVLTSA"
     gene            18915..19664
                     /locus_tag="VF225_04710"
     CDS             18915..19664
                     /locus_tag="VF225_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144589.1"
                     /translation="MTGSSEIEDDVVAEGVRVLSAARDQALPLRLLGGVAVRLRSRNE
                     LPAALQRTYGDLDFVTARRASGPAQKFFRDLGYSPQVSFNALNSRERLLFFDEDHGRQ
                     VDVFVGSFRMCHEIPLEGRMELDETSIPLAELLLTKLQVIQLNEKDVRDAIAVLHDRE
                     LGDEDGDTVNAGRIAQLCCEDWGLWRTITRNLEACRKQAGAYGEDLADVATRFSELEE
                     RIEAEPKSRAWRLRAKIGERKRWYDLPEERA"
     gene            19661..20602
                     /locus_tag="VF225_04715"
     CDS             19661..20602
                     /locus_tag="VF225_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144590.1"
                     /translation="MRLFFATDIHGSETCWRKFLNSGLHYEADVIVLGGDMTGKALVP
                     VVDEGDGRWHATLLENREELVGEDEVVAFEEAVIRRGYYPFRTTSEEVRELQDDEARW
                     HGLFEDEMLKTVERWMQVADEKLAGKGIRCFVCPGNDDQLNVDDVIRGAKSVELAEGE
                     VVEIGGYQMVSSGWANRTPWDTYREEDEEDLEIRIRKITDQVTAPPERTIFSLHCPPY
                     KSGLDDAPQLTKDMHLKEAGRSTVPVGSTAVRAAIEEHQPCLGLHGHIHEAKGTTRIG
                     RTLCINPGSSYEQGQLLGAVVDLDGKKKVKRFILTSG"
     gene            20614..22230
                     /locus_tag="VF225_04720"
     CDS             20614..22230
                     /locus_tag="VF225_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7144591.1"
                     /translation="MATMSGPAQAGEQALFVRKATGLVKGWSVRDAFIYSAISINFVT
                     LGLYAFSFANFIPRGSLFWAAILSGAYLIFQAITYASLIAAMPRAGGDYVWISRTLGG
                     GIGFVLAACGWWFILWHWVPIYANILNIMVLGPLSAIVGWDRGVTFWSSHNGVFTSSM
                     IVALMASVLVAAGVRAYARFQKICFYGGCVGLLIMFILLVVHSKSDFISAFNAQAHDV
                     YHTSGDAYAATAKAGSAGTYASLGSFHFGQIVLLIPILFFFNLWSNWGATLYGEVRGA
                     SDFRKNIYAMGGALLATTAVAAIFFGLFAHTFGWQWYHASNAAYWAGTGPLGVFPYPG
                     TLVAFFTGSAALQFIIVGILSLWFFGWVGTVFLSSTRVVFAAAFDRVLPEAAAKVSAK
                     GVPYGALALMLIPSIPISYFYAYNTKFSSWTFDATLVIAITFLGSAVSAAIFPWRRPD
                     IYNASPIAKYKVAGIPLITAAATAFTAILVFALYHWFKDSVYGVNFRNSEYYMAVLYG
                     VAIAVYVVSWLYRRSQGLSLKMVYGEIPAE"
     gene            22354..23526
                     /locus_tag="VF225_04725"
     CDS             22354..23526
                     /locus_tag="VF225_04725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7144592.1"
                     /translation="MSVEVETVRTVEVPTRIVHGAGAIARVGELVAELGVSRPLLVTD
                     KGVVAAGLADATLAHLENAVLFDEVRANPDIELVGRARTAYVEAGCDGLIALGGGSSM
                     DTAKSVGVEVVHGGSIVEYEYGGTPITTRIPPLVAVPTTAGTGSEATLWAVITDPERK
                     IKFNVGGTPLIGAHVALVDPELMLGLPPAITAATGMDALSHAIECYTCDYHQPFNDAV
                     ALLATELVARSLRVAYEDGGDLEARTDMAHAATLGGLAYGTESAGAAHAMSQSAGGVH
                     ECPHGALTARVLGPVCEYNAPADPERYARIAQGLGVDVHGLSPLEAALAGVDEVYRLT
                     DDVGIPSMAELGFSEEEIPMLARIAYEDPQTIGNPREVDATAYEEIYRNSFARGKR"
     gene            23550..24980
                     /locus_tag="VF225_04730"
     CDS             23550..24980
                     /locus_tag="VF225_04730"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7144593.1"
                     /translation="MYVGGSWVESESGERMEATSPATGETIGSVPEATRDDARRAIAA
                     ANAAAREWAARSAFERAAAMERVAEIIEERRDDLARTLTLDQGKPLRAESYGEVDELI
                     EYWRMAAGDARRLEGLMPPSVDASKRVLAYRVPRGVVGVITPWNWPYTMAAEVVAPAL
                     ASGNTVVWGPATSTSVCSVKLAECVVDAELPAGVFNLVTGPGSVVGDEIATNAGTHAI
                     GFVGSVATGYRVAERAAGKALLLEMGGNGPLVVLEDADLDGAVEATLAAAFLCAGQSC
                     TAGERFLVHEDVHDEYLDKLTAAVAKTIRLGDPFADETTMGPLNNERTAAKMDNHVGD
                     AVERGAVLVAGGARAREFPTDLYYEATVLDRVNDEMDVAKDETFGPVVPISTIRSEDE
                     ALAIIDSSPYGLLAAVFTNDLRRGLRFAEAARTGWVNINESTNYWESHLPFGGRAGSQ
                     SGIGRVGGRFSMETFTELKTVVVNLG"
     gene            25000..26211
                     /locus_tag="VF225_04735"
     CDS             25000..26211
                     /locus_tag="VF225_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX7144594.1"
                     /translation="MTKIVEHPEVLLYTRVRKSPYFYASRRHGVHMYSFYNHMYHPRH
                     YGDPVAEYWELLNGVTLWDVGVERQVEITGPDAFTLANMLVPRDLNKCAVGQCKYVFI
                     TAPDGGIINDPILLRLEENHFWISVADSDVLLWAMGVAYNSGLDVKIGEPDVGPMQIQ
                     GPKSRDVLVDLLGDSVLEIPYYFLKDYELEGMHLTVSRTGYTSEIGFEIYCRDAANNG
                     IKLWDLVLEAGKPHGLAVIGPCHIRRIEGGILAYGADMWLDTNPYEVDLGYEWMVDLE
                     QEADFVGKDALRKIKEKGISRKLVGVEIGGAKLGSYNDGSMMDFFPVSADGKNIGKVT
                     SACFSPRLEKNIGYAMVPIEYAELGTELEVERPEEKTSAVVVRRPFIDPEKEIPKQDL
                     AAAGARDESSS"
     gene            26270..27058
                     /locus_tag="VF225_04740"
     CDS             26270..27058
                     /locus_tag="VF225_04740"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX7144595.1"
                     /translation="MFPVEGIADDVVTHVPTEIKVAVTSSPARGIEATLRLCEELTSA
                     QFQVAPHLAARLVRDEEHLQDILQRLFAIGVRDVFAIAGDADLPAGKFDGATALLVAM
                     AELGHGLEQVGISGYPESHPFISDETTIQVMFDKEPFATYIVSQLCLDAGVIAWWIGA
                     VRARGVRLPIHIGIPGPIASTKLLRIATRIGVGESTRFLRRHGGLLARLFLRGRYGPG
                     SLIEQLAPQLADPASAIGGFHVYTFNELATTERWRREALRRLES"
     gene            complement(26997..28556)
                     /locus_tag="VF225_04745"
     CDS             complement(26997..28556)
                     /locus_tag="VF225_04745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX7144596.1"
                     /translation="MDLVSREEARERLGQRTFDLLVIGGGIVGAGIAAEAARAGLAVA
                     LVDRRDFGAGTSSASSKLIHGGLRYLRLGDIRLVREAHHERRLLMQVVAPHLVRRLPF
                     LLPLYRNGPYGRLTIQTGLVLYSGLARARLNRLVPPERAREHIPSLRLDGLRSCGLYA
                     DAATHDGRLCLANVRAAAEAGATVLNYAEVRGLRNVRGRVAGAEVLVDGTEVSVSARV
                     VVNATGPWVDEVRRLEDPAATPSVRLSKGAHVLLPLDEPWAAALTIPHDKVRVSFAVP
                     WQGMLLLGTTDTLFDRGPEEVQATEADVAQILGEAAVGIEADLLRPEAICSTFAGLRV
                     LPMGDGATTSARRETVFLTGPTGMLSVAGGKLTTYRRIAHAALTTLRAEFGLHRLDRG
                     PFPLPGAIDPVEGVRRLARRLPDLDVAVCTHLVHLYGSLADEVLAPAVADPDLLQPIH
                     PKGPDIGAQALYAREFEWAYGPEDVLRRRTTLALRGLAGENVVRRVDELFRTRGGEAP
                     RAASVPSSPVH"
     gene            complement(28547..>28806)
                     /locus_tag="VF225_04750"
     CDS             complement(28547..>28806)
                     /locus_tag="VF225_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144597.1"
                     /translation="LHLEKGSVEVLCSPCQRVTLCTSPRRGSSVSAGRFGYRYAGEIL
                     SEADDEEITCARLEWPAGSLYGRLELVDSYGRKAWTNPLWT"
BASE COUNT         4600 a         9682 c         9859 g         4665 t
ORIGIN      
        1 cgcgcgagca gggtcttgcc ggttccggga ggcccgctga ggagcacgcc ccgcgggatc
       61 cgcccaccga gtcgtccgta gcggtcgggg ttcttcagaa agtcgacgat ctccgtcagc
      121 tcgtccttcg cttcttcgat ccctgcgacg tcgtcgaagg tcacgcgctc cgcagaggcc
      181 tcgtagcgtt tcgctcgcga acggccgaaa gagccgagcg ccgagcgacc gccacgcctc
      241 atcagaagca ccaggaggcc gatgagcagg agggtcgggc cgaagccgta cagcaaggtc
      301 tcccaccagg cggctgagct ttccagcggc tcggcgttga tgacgacatt gttctcttcg
      361 aggagtttgg aaagtgcggc tgtgtccgcg aacgagggaa tcagcgtcga gaatcgttcg
      421 gagctccgag agccgctgcc ggttggcgga tagcgcacgg gacttttgaa cagcccctga
      481 actgatgtcc ccttggagat gatgcggtcg acgttgccct ggcgaacctc gttcaggaag
      541 agcgggctgt acgggatctg aacgcgtggc ggctccgaga gcgaacggga cgcaagccaa
      601 tagttcagga cgaacgcggc cagaatcgct atgacaaccc acctgccgac aggccccaac
      661 ggtcgtcgtg ggagtctcca gctcggcttg ggaacttcgg gagcgaccgg cagaggagcc
      721 tttctgccgg cactgctact cgggggggcc tcgcgcatcc actactaggc tgctcatcat
      781 gggcgtctcc gtcaagcttt tcgcgggtct cagggagcgc gccggctgga gcgaacgcga
      841 gctcgacggc gtcgaccgag tcgccgatgt ctggcccgcg ctcgatctcg gcgacgagcc
      901 ggaaggcctg ctctacgcgg tgaacaagga gtacgccgac cgagatcgcc cgctcgccga
      961 cggcgacgag gtcgcgctga tcccacccgt ttcgggcggt gctttccgcc tcaccgcgga
     1021 accgctgtca gtggatgcag tcgtcgacga ggtgcgcacg gaccaggcgg gggcgatcgc
     1081 gaccttcatc gggaccaccc gtctgcagtc acgcgggcgc actgtccttc acctcgacta
     1141 cgaggcttac gaagaaatgg ccgaacgggt gatggcccag atcgcggcag aactgaaaga
     1201 acgctatgac ctctgtcaga tcgcgatcca ccatcgcacg gggcgactcg agatcggcga
     1261 ggcgagcgtg gtcatcgccg tgtcggcgcc gcaccgacag gatgcgcttg ctgcgtgcaa
     1321 ggacgcgatc gacaccctca aagagcaggt gccgctctgg aagaaggagt tctacgaggg
     1381 cggcgaggaa tggatcggcc gcggttcgta gcttccgttg ggacagatca gcgccctgtg
     1441 ggccgcccgg cctattccga accggggcaa acactgcgag gtttaggtcg cctctcgccg
     1501 tcgggaggcg ggcatggaag acatcgacca gagccgattc gcgctgatga cagcgctagg
     1561 cgaggcccac aaggcgcgcc ccgacgagta ccccaacccg ggcaccctgg tcgccatgag
     1621 cgatgagggc gtccgcgcgt acgcagtcgg gctgctccgg ctgctcgagg cggcgccggg
     1681 tggcgacggt ctcgagactg agctgcagaa ggagctgcga ctgatcctgg gcgacactcc
     1741 ctagcccagc gttccgtctc gctgccctga gccggcgagc agtggatcgg ccgggggtca
     1801 tgcgctgccg cggtgcgacc gataccggtt ggcacaatgg cagggatgga gcaataccca
     1861 caggagtggc gccagtacga gccgatccag ccgaaaggct tcgactggcg cggccttttc
     1921 cgcaggatct gggcgccgat cgccgctatc ggcgcatttc tggccaagtt cggcgtcgtc
     1981 ctgctcaagt tcaagttcct cttctcgatg ttcgtctcgg ccgctgtata cgtctggatc
     2041 ggcggctggt ggttcgggat cgggctcgtc gtcttgctgt tcgtgcacga gatgggacac
     2101 gtcctcgagg cgaagcggca ggggctcccg gtgtcggcgc cgctcttcat cccgttcatg
     2161 ggcgcgctga tcacgctcaa ggagatgccg cacaacgcct ggcgcgaggc aaagcttgcg
     2221 atcggaggcc cgctgctggg ctcggccggg gcgcttgcca tctatatcgc cggcgtcgcc
     2281 tatgactcga actccctcaa ggctctcgcc tttctcggct tcttcatcaa cctcttcaac
     2341 ctgcttccgg tcgtcccgct cgacggcggg cggatcacgg ccgccttgca cccggcgctc
     2401 tggttggtcg gcttcctagg actgctgggc ctcgtgatct ggaagcccaa cccgatcctg
     2461 atcatcatcc tgctcttctc ggcgtctgag ctctggcgcc gctggaagat gcgccatcac
     2521 cccgagatgc aggagtacta ccgcgtcaaa ccgcaccaac ggctgatcat cggtgtgctg
     2581 tacttcggcc tcgcgatcgc actggtgctc ggtatgcacg cgacgcacgt gccgcgaaac
     2641 ttctgatgga agacagacgg atcctctccg ccgaggagaa gaccgaagat ctcgaacagg
     2701 acatcgccct catcgcgcac gagttccgcg agggcttcga ggcggtcgac atgatcgtcc
     2761 agccggcggt aaccatcttc ggttccgcgc gcatcaccga agacaacccg gtctacgcgg
     2821 aggcgcgcac ggcaggccgc cgcttcgccg agctgggctg ggcggtcgtg accggcggcg
     2881 ggcctggcgt gatggaggcc gccaatcgcg gcgcgaagga aggaggcggg ctctccgtcg
     2941 gcttcaacat cgaactggcg cacgagcagg cgccgaaccc ctacctcgac atctcgctga
     3001 cgttccggca cttctacgca cgcaagacga tgtttgtgaa ggccgccgaa ggcttcgtcg
     3061 tcttcccagg tggattcggg acggtggacg agctgttcga ggcgctgacg ctgatccaga
     3121 cgggaaaggt gttccacttc ccggtcgtcc tgtacggcag cgactactgg ggcgagctga
     3181 tcgactggat caagggcgaa ctgctcgccg acgggatgat ctcgccggag gatctcgacc
     3241 tcctgttcgt caccgatgat ctcgacgagg tggtcgaacg agtgcgggcg ggctacgagt
     3301 cgcgctcctc ggcgacacct gctgccccgg tcaaggccga cgcgcagtga tcgcgcctta
     3361 gttacgaagt agcctctcgc catgaggttg cgtgaccttc cgtccgttga cgagctggcg
     3421 cggcagagcg ccgatccgct cgctgtcgag gctgcgcgca aagtcgtcgc gcgcgcgcgt
     3481 gaacagatcc agcggggtga cgatccaggc gatctggcgg cgctgctgca gaacgaactc
     3541 gacgctgcgc atacgtcgca cctgcggcgg gtgatcaacg ccacgggcgt gatcgtgcac
     3601 acgaacctcg gccgtgctcc gctcgccgag gccgcgctcg accgtgtgcg cgagatcgcg
     3661 cgaggctact cgaacctcga gtacgagctg gcggccggcg cgcgcggctc ccggcaggaa
     3721 cacgtcgccg acagcctccg ccgcctgacc ggggccgagg ccgcgctcgt ggtcaacaac
     3781 aacgcggcgg cggtaatgct cgcgctcgcc gcgctcgccg aaggtcgtga ggtgttggtg
     3841 tcgcgcggag agttgatcga gatcggcgac gggtttcgca ttcccgacgt actcgaacga
     3901 tcgggtgcgc gcctgcgcga ggtcggaacg acgaaccgca cgcgcgcatc cgactacgaa
     3961 gccgcgatcg gcacggagac agcggtgctg ttgcgcgtgc accagtcgaa cttccgtgtc
     4021 gtcggcttta ctgcccagcc ttcgctgggc gaactggcgg acatcgctcg ccgtcacgat
     4081 ctgccgctcg tcgacgatct cggatcaggt gcgctcatcg acatcggtga cgagccgacg
     4141 gcccgcgcaa gcctggctgc cggcgcggac ctcgtgtgct tctccggcga caagctgttg
     4201 ggtggccccc aggccgggat cgtcctgggg cgcgccgagc tcgtggagaa actgcgccgc
     4261 catccgctgc aacgagcatt gcgcgcagac aagctgactc ttgctgcgct cgaaggaact
     4321 cttgcgctcg ccgtcgacgc gccggatgag atcccggtgt tgcgcatgtt gcgcgagccg
     4381 gtggaagccg tgagagtgcg cgccgcgcgt ctggcggagc tcgttcacgg tgaggtcgaa
     4441 gagaccgttg cacgggccgg cggtggatcg ctgccgttga cggagctccc aagttttgcc
     4501 tgcgccgtag aggaagagct ggccgtgaag ctgcgggcgg gcgatccacc cgtgatcgcg
     4561 gtcgtgcgcg acggccgaac gctcctggat tgccggacgc tcacggatgc ggaggcggac
     4621 gaggttgcgg tggctgtcga ggccgcgcgc gcatgaacga cgtgccgccg gaagcacggc
     4681 tttggaacct catccgcggt gcgttgggca ccaaggccct cggcatcgcc gcggacctcg
     4741 gcgtggccga tgcgctcgcc accggtccgc gctcggttgc cgagcttgca gaggaaaacg
     4801 gtgcggatac cgacaccctc catcgcatcc tgcgggcgct ggcgagcgac ggagtcttcg
     4861 cagaggacga gcccggtgct ttccgcaaca ccgaggcctc ggagctgctc cgcagtgaca
     4921 acgacgaccg ctggcgcgag ttcgcgcacc tcttcggtga ggtctgctac cccgcaatcg
     4981 gtgcactcga cgtgcgcctc accgacgtgc cctttgcgcg cgcgtacggg acggacatct
     5041 ggtcctggtt tgccacccag ccggaggagc gctcgaattt cgaccgggcc atggagggcg
     5101 gccaggccga acgtgccgac aagctcgctg cgctcgagtg gcgcgagggc gaggccgtcg
     5161 tggacgtcgg cggcggcacc ggcaggctcc ttcgtgacct cctcgagcgc cggccggagc
     5221 tgcgcgggat cgtcttcgac ctgcccgagg ctgatcgaga ggagaccgcc gtcggtgatc
     5281 gcatcacctt cgtttccggg agcttcttcg agcaggtgcc cgaaggcgat gcctacgtgc
     5341 tgtccaagat cctccatgac tgggacgacg agcgtgcggc agccgtcttg cgtaccgtcc
     5401 gcgcagcggc accggatgag gcgcggttgc tcgtcctcga ttcggtcgtc ccgccgggca
     5461 acgaccaggc cggcgtgaag tggctggacc tcttgatgct cgtcctccag cgcgggcgag
     5521 agcgaaccga gtctgattgg cgtgcgctcc tcgacggcgc aggcttccgc gtggagcaga
     5581 tcgaggacgg tctgattcaa gccagatgcc gctgactgtc gggacagcag gacacatcga
     5641 ccacggcaag acatggctcg tgcgcgcgct gaccggcaag gacaccgatc gacttcccga
     5701 ggaacaggcc cgcgggatct cgatcgatct gggctacgcg ccgctcgagt tgccggacgg
     5761 ccgccggctc tcgctcgtgg acgtgccggg acacgagcgc ttcgtccgca cgatggtcgc
     5821 gggagcgacc gggatcgacc tgttcctgct cgtcatcgac gctggcgagg gcgcgcgtcc
     5881 gcagacgcgc gagcacctgg cgatcctcag gttgctcagc gtcgatcgag gtgtggtcgc
     5941 agtcacaaag gcggatgccg tcgagcccga tgcgctcgag ctcgcactcg acgaagcacg
     6001 cgagctcgtg ccgggctcgg aggtcgtcgc cgtcagcgcg aagacaggca gcggcctgga
     6061 cgagctgcgt gctgctctgg ctcgcgcctc cgtcgacacc gaacgcgcgg acgcaccgac
     6121 gaggctctat gtcgatcgcg tcttcagcct gcacggcatc ggaacggtcg cgaccgggac
     6181 tctctggtcg ggaaggatcg gcccgggagc catcctccgt gcagagccgg gcgcgcgcga
     6241 ggtacgagtt cgcagcgtgc aggtccacga ccgggaagtc gacgtcgccg aggctggaca
     6301 gcgagtcgcg gttgctcttc ccggcgtgga gcggagcgac ctcggccgtg gcgacgctct
     6361 agtcgaacgt gacgcgtacg acgtcagctt tcggctcgat gtcgaggtcg aggagctcgc
     6421 gccgatcccg ccgcgtgtac tcgttcacca cgggacggca accgtgctcg cgcgcgtggt
     6481 gcgcgtcgac cgcttcgccc agcttcggct cgacgcgccg atcgtggctg cgcggggtga
     6541 ccgtgtcgtg ctgcgcgcgg gaacgacggt cggaggcggg ctcgtcatcg acgccgcccc
     6601 gccgcgtcat gctgacgcga cgcgattcga gcgcagcgcg cgcggcgagg tgctgctcca
     6661 cgcgccggtt ctcgatgccg acggcggctg gtctttctcc gatgaatggc tcgaagagct
     6721 tcgcgttgag ctcgagcggc gcctcgacgc ggctgacccg ctcgatccgg gcgtgccggc
     6781 tccgacgcag gcctgggcgc cggccgtcct gccgcggctc ggactggagc gacgcggcgc
     6841 gaagctctac cgcccgggca gggcgggctc actcggcgag cgcgaacgag aggcggccga
     6901 actggaagca cggctcgggc tcgagcccgt caaggtcgaa gacgcgggcc tcgcgcgctt
     6961 cctcgagcaa cagggtcgac tggtgcgcac cggcgatgga tacgccgtct ccgccgctgc
     7021 ttacgagcgc gcccgggatg tcctattcga agaactcggg gcgtccggac ggatcacgct
     7081 cgcccgcttt cgggacctgc tcggagtgtc gcgcaagacc gcacagctgc tcctggagcg
     7141 tttcgatgca gacagagtca cgcgccgcga cggcgatgcg cgcgtcttga ggcgcagcgc
     7201 gccgcgcccg taggacccgt cggacgaggg ataaggccgt ccggattgac ggccgatgct
     7261 gatcgtggcg taagtcctca actgcggaaa ggcggcatcc gatgctcgaa ctgctgaaga
     7321 gcaagcttcg tggttttccg accggaaacc tcggcctgcg gatcggctcc tagcggatct
     7381 gaaggcgcaa cacgtccggc cggttgtaat gcccggccgg gtcgaaacgc tgacgtgcct
     7441 cgtagagccg ttgtggatcg agatcggcgt agagaatgcc ttcctcgttc cacagcggcc
     7501 cggcgaggta ggtcccgtcc ggcgcaagaa tggctgagcc cccggcgccc accagctcgt
     7561 caccatcggc aagcggcacg tcgtccgggt acgcggaagc gcgctggaag acacagcagc
     7621 tgaggacgaa tgcgcgcgac tctcgcgcga tgtgcttcat cgagtcgtgc cagtcctccg
     7681 aatcgtcggc cgtcggggcc agatacacct cgactccacc ctggtagagg gcgaagcgcg
     7741 cgagcggcat caggttctcc cagcagatca ggccgccgac cttgccgagg tcggtcggga
     7801 tcgtctccag cccacgccca tcgcccagtc cccagaccag gcgctcgtga ttcgtgggca
     7861 tcagcttgcg gtggtggagc gccagccctc cgtcaggtga gtagacgagc agcgagttgt
     7921 agatcgtccc gccgtcgagc tcgttcacgc cgaccgcgag ccagaggtcg ttcgcacgcg
     7981 cgatctcggc gaggcgatcc gagtcggcgc cgggaaccgt caccgactgc tgcatcagtc
     8041 gcgcgaagac ttcgcgtgct gctcccgcct ccggaccgcc ccagcccgcg aggtagcgaa
     8101 cccagcggtt ggacgggtag acggggatga aggtctccgg aaagagggcg agtcgcgcac
     8161 ccgctcctgc gacttcgcct gcgagccctg cgaccttgtc gatcgtggcc gcgcgatcga
     8221 acgcgaccgg ctcggcctgg acgcatgcga ccttgacgac gccgtccacg ggctgagcct
     8281 atgccttcgg gagatctgcg acgagctcgt cctcgtcctc gtagtcgaac ttgaggtaat
     8341 ccgcgccgcg cgtgagcacc tcgcgccagt ctcgatcccg cgtggccccg atcaaccagc
     8401 ggacctgccg gggaagcgcc tccgcccagg tggtcgcagc gtggtaaccg agctcctgct
     8461 cggccttcgc catgtcaacg agcataggct tggcagccga ccagggcgtc tgaccgacct
     8521 cgccgcgagc ggccggctct tcgagcagaa cctcctcgaa gcgatggcct gcggcttcgc
     8581 cgatcgcgcg ggcaatctcc agcacggtcg gcggatcggg atcgccgcag ttgaagacac
     8641 ccgtccgcgg ccgggccgcg atcaaccgca cgagctcgcc gatgttctcc gacgcggtgg
     8701 tgtggaactg tccggcaccg cggttcgtca gcacgacgaa gggccggcga tcgagcgcgc
     8761 gcttgacgaa gtgccactcg cgccccatcc ggtcgccagg cccgtagatc gcacacggcc
     8821 gcacgatcgc ggccggcacg tggccgttct cgagcaggat ccgttcgagc ttcaccttct
     8881 tcgtcgagta cgtgtcgtcg ccgggcgcga ctgttggctg cgtctctggg atcgggagcg
     8941 ggaactcggg gaagtcgtcg acgccctgcg cctcgtcgag cgtgcggcct tgctcgtcgg
     9001 cgtagacgga cgcggaggag atcgcgacga gcgcgccggt ctcgagcgcg agcagctgtt
     9061 cggcgtgcgc cgcctcgaac gggatgacgt cgacgacgac gtcgaagccg tccgcgacgg
     9121 agagtgcgcc ttgctcgtct cggtcgagtg cgacctgttc gatacccacg ggcgcggccc
     9181 gctcggttct gcccgcaacg gtgacctgcc agccgtcagc tgcgagcgaa tgcgcgatcg
     9241 ccgtgccgac gttgcccgtg ccgccgagga tgaaggcgcg gccgactatg ggtgtctaga
     9301 ccgcgacgac cggatcgccg atgctgatct cgccgtcgct gagaatgtcg gcgttgagac
     9361 cgccgcgatg cacgagcgcc ttgatcaggc ccggctgcgt catcgactcg agatgcgcgc
     9421 agggctcaca gagctcgacg ccttcgcatt cgacgtcgcc gatgcggaag cgcttgccga
     9481 cgagctcgtt gacgtcgatc ccgcgcgtaa gtacgttgcg ccgcgttgcg gcgggctcga
     9541 tcgagacgtt gccctcgagc gcgaccgcat ccatcgcctc ggccgcgatc agcgtcacgc
     9601 ctcgccccgg gggcgcgtcg ccgtcttccc agaagtaacg gttgccctgg agaccgcgtc
     9661 ctgcgtaagc acgaacgcgc tcgacgggag ccgggagctc gccccgctcg gacgcgacga
     9721 agatcgcttc gacatggccg ctcacaggct agaccctacc caccatgccc gccgccgccc
     9781 ggggtctcga tcgtgacgac gtcccctgcg gtgagatcgc gcgtcacctt cgccggtagg
     9841 ggctctccgt tgagcgagtt tcgcccggcg gctcccgggt cgcccccgcg ctcgccctgc
     9901 ggccggtgca cgcggcgctc gctgatgatc gagagcctgc aggcctcgag gacacgcagc
     9961 tcgcgcagga cgccgtcgcc gcctcggtgc cgtcccgctc cgccggagcc gagccggagc
    10021 ccatagcgct cgacgcgcag cgggtactgc agctcaaggg cttcgaccgg cgtcgagagc
    10081 gtgttcgaca tcgcgacatg cacgccgctc ggcccatctg catccggaca tgcgccctga
    10141 ccgccgccga tcgtctcgta gtacgtgaag cgttcgttgc cgagcgtgac gttgttcatc
    10201 gttccctgtc cctgcgccgg aacctcgatc gcctcgccga aggcgcgcat cacgacgtcg
    10261 acgattcggg aggaggtctc cacgttgccc gcggccacgg ccgccggcgg gcgcgcgttg
    10321 acgaggcagc ctgcgggagc ggtgacgttg acgggggcga acgcgccgcc cgaccccggg
    10381 agagcgggat cgacgagaag gcggacgacg tagaagcacg cggagcgcgc aaccgacagc
    10441 gggcagttga gattcccggc atgctgcggg gaggttccgg tgaagtcgat tgcgaccgtt
    10501 tcgccctcga ccgtcacggt cgcgtgcacc tcgaggtcgc ctgcgatcgc ctccagcacg
    10561 tcgtgcgctg caaaccgacc gtccgggaga gcggcaatgc ctgcgcgcat cctgcgctcg
    10621 gagtaggcga agagctcgtc catcgccgcg atgaccgtgt cgcgtccgcg ccgctcacag
    10681 agctccgcga cccgctgctc cgctaggcgg tgcgccgcga gctgcgcgcg cagatctccc
    10741 tgccgctcct cggggttgcg catgcgcgcg accagacccg cgatcacgtc gtcgtcgagc
    10801 cgcgtcggag gaatcacgac gccttcttcg tccagggtgc gcgaatcggc gggcatcgag
    10861 cccggctccc ggccgcccac gtctgcgtgg tgcgcgcgcg tgaccgcgaa gcccgtttcg
    10921 gttcgcgaca cgagcgtgat gtccggaagg tgcgtgcccc ctgcgtaggg atcgttgagc
    10981 gcccacacct cgccgaaggc cgggtcctgc cgcatcaccg cggcgaccgc atcgggcatc
    11041 gcgccgagat gcacggggat gtgctcggcc tgcgcgacca tccggccgcg ctcgtcgaac
    11101 agcgccgtgg agcaatcccg gcgctccttg atgttcgccg agaacgccga acggacgagc
    11161 acggcgccca tctcttccgc caccgcgcgc aggaggctgc cgatgatctg gagctcgatc
    11221 gtcactgtcg cgtgagcctc agcatgcgac catccctagc ccccacccac ccagggggaa
    11281 tccagcacgt ggagccggcg agctcgacga cggcggggcc gtagatcgtc gtttccttgc
    11341 cgcgcggcag ctcgagctcg ggtccccgac ggatgtcggc ggtccgcaca gccaccagct
    11401 ggacttcgcg gtcccgctcc gcgaacccgt actgctcttc gtgtgcgcgg tgaaatgcgt
    11461 cggccaaatc ttcctgcagc ggcacggtca gttcgaagga ctgtcccgcg tagcggagat
    11521 cggcttcgcc ttcccgcggc agctcgccgg cgtcccgaag ggggacgaga tacgagcgca
    11581 ccgcgtctcg ccgctcctcc gacgcgacca gtccgagtgc cgacagcacg cccgcggcct
    11641 ccgggacgag cgcggtgcga atgccaagct cgtccgccag ctcgcacgcg tgcagtgggc
    11701 cggctccgcc gaacgccacg agcgcgaagt ccgccgggtc gtgcccgcgc tccacggaga
    11761 cgacgcgcag cgcacgcagc atctccgcgt tgacgacatc gacgaccgcc gccggatcga
    11821 ttcccgcgag tgcgcgctcg gccgccgcgc ggtcgagctc gacgccgccg gcgagctggt
    11881 gaggcagtcg gccgagcagg aggttcgcat cggtgaccgt gccctgcgtt ccgccgcgtc
    11941 cgtaacacgc cgggcccgga tctgcgccgg cgctctgcgg accgacatgc agtgcaccgc
    12001 cggcgtccgt ccagacgagc gagcccccgc ccgcaccgac ggtgtgcagg tcgaccgtcg
    12061 agaggcggat cggcaggcct gcgacagttc gttcgcttgc gcgctgggcg cgaccgcccg
    12121 cgatcaggca cacgtccgtc gacgtgccac ccatgtcgaa cgcgatcgcg ttctcgatgc
    12181 cgtccagtgc cgcgagtcgt gcagcgccga ccacaccggc ggcggggccg gagacgagaa
    12241 tcgtcgccgg atgatcggcc gcttcgtcgg gggtcgcaac ccctcccgag gaacgcatga
    12301 cgagcggctc cggtaagcct gctgccgcgg cagcgtctgc cagcgcgtgc aggtagcgcg
    12361 ccacgaccgg cccgaggtag gcatcggccg cggtggtcga cgcccgctcg tactcccgga
    12421 actccggcgc cacctcgtgc gaggcgacga cgtgcgcatc cggcagcctg cgccggagct
    12481 cctcgacaac gacgcgctcg tgcgacggat cgcggtacgc gtgtaagagg cacaccgcga
    12541 ccgcgtcggc gtcgatgtcc ggcagcgtgc cgagctcgag cggctcgagc tctccctcgg
    12601 gacccatgcg cccgcgaatg ccgtatgagc ggtcgagcgg aacgagcggc tcgggccacg
    12661 ccgcacacgg gcggtagagc tgcgcgcgtg tctgacggcg cagatgcagg agatgctcga
    12721 agccggcggt ggtgacgaac gctgtccgcg ctcccttgcg ctcgagcagc gcgttggtcg
    12781 caaccgtcgt cccgtgcgcg aaacgctcga cgcggtcggc gcccgccgct cgcgctgccg
    12841 cgaggaccga ctcctcctgg tgaggggcgg tgagcacctt ggcggtggtc acgcgaccgc
    12901 cgtccaggac gaccgcatcg gtgaaggtcc cgcccacgtc gacgccgagc acagccattc
    12961 aggaagactg acagcgtggc aatccgtggg ctcctcttcg acttcgacgg cctgctggtc
    13021 gacaccgaga ctccctcccg gctcgtctgg gaggagctct accgcgagca cggtcacgag
    13081 ttgccgcagg atcggtgggc caccctcatc ggaacgatcg gcgcgccttt cgatccgctc
    13141 ggacatctcg aggagctcgt cggcaggcgc ctcgacgagc aggcgctgac cacacgtcgc
    13201 cgcgcccgcg agtacgagct cacggatctc gaagagctca gacctggcgt ggaagagtat
    13261 ttcgccgaag ccgagcggct cggcctgcgc accgcggtgg tgagcagcag cgacgacgac
    13321 tggatcgcgc gtcatctcgg ccggctcggt cacctcgaag gcctcgacac gatcgtggcc
    13381 gcaaacggcg acacggcgcg ggcgaaacct cgacccgatc tctacctcga ggcgctcgac
    13441 cgtctcgagc tcgagccgca cgaggctatc gccttcgagg actcaccgaa tggcgtcacc
    13501 gccgcgaaag cagccgggct cgtctgcgtg gctgtgccga acccgatgac ggcgacgctc
    13561 gcgctcgacg acgccgacct cgtgctcgaa tcgcttgcag acgtccccct gcgcgggttg
    13621 ctcgaacggt tccgatcggc gcgatgaagg cattgacgca gatgaagtgc gtcgcctgtc
    13681 gcaaggacgc cccgaccttg gccgatgccg agatcgcaga actgcatccc cagattcctg
    13741 actgggaaat cgtcgagctc gacgggatca aacgtctgag acgcgtcttt gcggtcgatg
    13801 attttgcgca ggcactggaa ctcacgaacg aagtcgccga gcttgcggag caggagcaac
    13861 atcacccggc gctgctgacc gagtggggca ggacgacggt cacgtggtgg acgcacaaga
    13921 tcaaaggcct gcatcgcaac gacttcatca tggcggcgaa gacggaccag ctgtacctgc
    13981 gctactagac cccgttcttg cggagctccg cgacgatcgc gggcacgatc tcgtggaggt
    14041 cgccgatcac ggcccagtct gcgcgcgcga cgatcggtgc gctctggtcc ttgttgatca
    14101 cgaggatccg cttggcggcc ttgcacccga cgatgtgctg gattgcgccg ctgatgccgc
    14161 aggcgatgta gaggtcgggc gcgatccgcg cacccgtctg tccgacctga tcggagtgcg
    14221 gtctccagcc gaggttggtg acgacgcgcg agccgccgac ggcacccccg aggaggttcg
    14281 cgagctcctc gagcagttcg aacccctctt tgctgccgac gccgcggccg ccgccgacaa
    14341 cgacgcgcgc ggtgtcgagc cgaacggtgc cggcggtcgg ttgctcgcgg cctgacaccc
    14401 gcacgacgaa gtgttcgtcg gcgagggacg gggtgaactc ctccgtcgtc gtagtgacgc
    14461 cggcgggctg cgccacgacg gcatgcgcgg cgaccgtgag cagcttcggc gttccggtga
    14521 gccgggcctc ttccagcagg ctgccgcccc agcgctggcg cgtgagctgc aaggcgtcgc
    14581 cgtcggttgc agccgcgacg acattcgcgg ccagtgggac gcccagccgc gccgcgacgt
    14641 gggccagcac ctcgtgcccg cggtcggtgc ccggggcgac cacgacctcc ggctcgagct
    14701 cgacgatcgc ctgcgcccac gcctcgggcg cgtagtcccc aagtcgcggg tgcacaacgc
    14761 ggcgcgcgcg tgcggtgtag ccgtcgacgt ccgtctcctc gaagatcagc gcctcgacgt
    14821 caccgagttc gcgtgcgaat gtcagcgctt cgagcgagac cgggtcgccg tcgacgaggc
    14881 agaggatcat gacgtcacca ctagcccgag ctcgcgcagc acctccacga cgcgcggcgc
    14941 cgcggcggcg ccggcgagga tctcggcgtg cgtctcctgc tccggcggga cgacgagcct
    15001 gatcgtctcc agcgcgctcg cgcgcggctg tggctcgatc cgcgcgatcg gcgtcttctt
    15061 cgcacgcagc cgcccgggca gcgacgcgta gcgcggcaga ttgattcctt ccttcaccgt
    15121 gacgaccgcc ggcagcggca gctcgaagat ctcccagccg ccgcctgcct cgcgccgcgc
    15181 tgtcagcttc ctgtcctcga tcgcgatgcc cttgatgccg gtgacgcacg ggagccccag
    15241 cgcatgagcg acgcgaacgc ccacctggta gtcgccggtg tccgccgcct cgttgccgaa
    15301 cagcagcacg tcgaacagtt cgggttgcgc gctaatcgcg tctgcgagtg cagcggccgt
    15361 cgcctcagcg ttccagtcac tcccgtcggt ctcgagcagc acggcgttgt ccacgcccat
    15421 cgccatggcg ttctgtagct gttcggcggc gtcgggcggt cccagggtca gcaccgtcgt
    15481 cgaccccccg tgcttcccga cgatgcgcag cgcttcctcg acggcgcact cctcgtgcgg
    15541 cgagatcgtg aagccgagca ggctcgtgtc gatcgcctgc gcgtcgcccg tgagcgtgat
    15601 gcgtgccccc gtctcgggaa cgcgcttcac gcacacgatg acgttcaact ccgaacccga
    15661 gtgttctctg gatcgaagag cgggcggctg cccttgaccg cgacgctgac gggatagcgg
    15721 tcgcccatgt attggaccag gagcttgttg ccctcctccg catgctcggg cggcaggtat
    15781 gcgagcagca cgtgcttgcc cacggagggc cccgagcccg cgctggtcac gtaagagcct
    15841 cgtcctttgc tgtcggcgat ccgcgcgccg tccgccgtga ggatcggctc gcgcccgagc
    15901 atgtagcgct tctttccgtt cgagggcgag gtgttgtcgt cgacggtgag cgtcgagaga
    15961 atcgccgcgg gctcctcgtt ccggtggcgg acgtgcgcgt ccttgccgat gaagtcggca
    16021 tccttcacct tcggccgctg catgtcggcc tcgacgacgt tgtattccgt ctccagttcc
    16081 gcgccgtaca accggtagcc cttttcgatc cggccggtcg tgccgtagac gccgatccct
    16141 gacgggacga cgccgaacgg ctgtcccgcc tctcggatcg tgtcccacag cgcggcgcct
    16201 tgctccatcg gggcgtacag ctcccagccg agctcgccga cgtacgagat gcgcgacgcg
    16261 agggtacgta ctggcccgat gtcgatccag cggcagcgca agaacggaaa gccctcgtgt
    16321 gagacgtcgg cgctcgtggt tgcttccagg atgtcgcgcg cgcgcggccc ccagaggccg
    16381 agcgtgcacc atgcggaggt cacgtcgtcg agcgcggcgg agccgtccgc gggcagctgg
    16441 tcctcgaacc acttgcggtc gcgcggaccg tccaggccgc cggtgacgac ccggaagaag
    16501 ttgtcgccga gacgcatgat cgtgaggtcg gccttgaagc caccgtgctg gtcgagaatc
    16561 gacgtgtaga cgactcggcc gaccgcgacg tccatctccg caagcgccag gcgctgcaag
    16621 tgttcaagcg ctccggagcc gacgatgtcg aagacggcga aggccgagat gtcgatcatc
    16681 gccgcacggt cgcgcatcgc aagatgctcg gcgttgatga tcggcgacca ccagcgcgat
    16741 tcccactccg cctcgcgagg catcacgcgg tcgccgtact cctcgagcag cggctcgttc
    16801 gacgcgtacc accacggacg ctcccagccg gcggtctcgt agaacaccgc gctcagctcg
    16861 cgctcgcgcg cgtagaacgg gctcaaccgc acctcgcggt tcgacagcca ctgctccatc
    16921 ggatgaacga tcccgtaggt gcggttgtag ccctccgagg agcgtgcgtc gacgtggcgc
    16981 ttcgtgcgct gatgcgggta gaagcgcgcc acgtccgcgg tatgcagatc tgtctccggc
    17041 gcgccttcca cggcccattc ggcgacggcc ttcgcgatcc cgggtgcctc cttgatccag
    17101 atcgcggcga cggaccacag gttcttgacc tccgtctctc ccagcagggg catgccgtcg
    17161 ggagtgagcg acagcagccc gttgatcgcg tgccggatgc ccgctccctc gacggtgagg
    17221 atctccggga agagctcgag tgcctgctcg agctgcggat cgaagtcctc ggacgtgaac
    17281 ggcagctcgg tcggcgacag cgcggactcc tcgatcgatg ggatctggtc gggctccagc
    17341 agaatcgcgc ggtgcgcgta cgagccgacc tccatgtcgc ttccgttctg gcgctcgtac
    17401 atgttcgtgt ccacgtcgcg cacgatcggg aaggagatct cgccgggcgt ctcttcgaag
    17461 agcgggatcg gtcccacgct gatcatctgg tgcacagccg gcgtcagcgg gatcgtcgcg
    17521 cccgccatgc gcgcgatgcg cgggctccag atcccgcacg cgacgaacac gtactcggcg
    17581 tcgatcgagc ctttgtcggt gtggacgcgc cgcacacggt tgttctcgac gtcgataccg
    17641 gtgacctcgg tgctggcgaa cacgctcgca ccgcgttcct gcgcgtactc gcgcatcagc
    17701 gtgccggcgc gcaacgggtc gacgacggaa acgcccggcg tgtagaagcc gccgagaatg
    17761 acgtccgtgt tgaggaacgg caccttcttg agcacctgcg ccggcgtcag cagctcgcac
    17821 ccctcctcgc cccaggagag cgccgacgac atccgccggt tcagctcctc catccgctcc
    17881 ttcgtccggg cgacctcgac gccgccgctg atcgtggcga gcccgagctc gatgtactgg
    17941 cgggcgctct ctttcgtgag cgtcgtcatc tccttcgagt ggtcgacagg gaagatgaag
    18001 ttcgacgcgt gacccgtcga gccgcctggg ttgggcaggg gaccctgctc gagcaagacc
    18061 agatccgtcc agccgagccg cgagaggtgg tacgcgatgc tgttgccgac gatgccggcg
    18121 ccgaccacta cggcctgcgc ggatgacggt agagggatag tgctcacgcg ctcgtcagca
    18181 ctcgccgcat gacctcttcg aagtcgagca cgtgccggcg catcgtcgcc tcggcgcggt
    18241 cggcgtctcc gtcacgaatt gcctccaaga gctcgcgatg gcccaacacc gcctcctcca
    18301 gctccggggc gcgatcgagc gcgaggtacc agatccgcag cgccaggacg tagtactcct
    18361 cgagtgtggc ctcgaggaag gggttgtggg cacagctgta cacctgacgg tggatccgct
    18421 cgtccagggc catcagctcg cgcgcgtcca gctcgctctg gcctcccaac tcgccgagga
    18481 gggtctccaa cgcgttgcgg tcttcgtcgg tcgcccgctc ggcggcgagg cgcgctacgt
    18541 ggctttccag agccgatcgc acctcagcga gactggccag atcgcggatc tccacgctcg
    18601 tgacgaacat gccccggcgg gggtagacct cgaccaatcg ctcctgcgag aggcgacgta
    18661 gggcctcgcg ggtcggcgtg cgcccgatcc cgagtcggtc catcaacccg cgctcgtcga
    18721 tcaccgcccc gggccgcagc tcgaccgaaa cgatcatctc ccggatcgcg tggtacgccc
    18781 ggtcggcgag cgaggccgtc gccccgctcg aggcctggag gacgctgacc tgccccttcg
    18841 cctctgccac ggagatacat tactcatatc tcagttcgtt cccatccgca ccagaacggg
    18901 acgtagagga cgccgtgacg ggctcttctg aaatcgagga cgacgtcgta gccgagggcg
    18961 tccgggtcct ctcggccgcg cgcgaccagg ctctgccgct tcgtctgctt ggcggcgtcg
    19021 ctgtccgcct caggagtcgg aacgaattgc ctgcggcgct gcaacggacg tacggcgacc
    19081 tcgacttcgt taccgcccgg cgtgcctcgg gtcctgcgca gaagttcttc cgcgacctcg
    19141 gttactcgcc gcaggtttca ttcaacgcac tcaactcgcg ggaacgcctg ctcttcttcg
    19201 acgaggatca cggccgccag gtcgacgtct tcgtgggctc gttccgcatg tgccacgaga
    19261 ttccgctcga aggacgcatg gagctggacg agacgtcgat cccactcgcc gagctcctgc
    19321 tgacgaagct gcaggtaatt cagctgaacg agaaggacgt gcgcgatgcg atcgcggtcc
    19381 tgcatgaccg tgagctcgga gacgaggacg gcgacacggt caacgcggga cggattgcgc
    19441 aactctgctg tgaggactgg ggcctctggc ggacaatcac ccgcaacctc gaagcgtgcc
    19501 gtaagcaggc gggtgcctac ggcgaggatc tcgccgacgt tgccacccgc ttctcggagc
    19561 tcgaggagcg gatcgaggcc gagccgaagt cacgcgcctg gcgcctgcgc gcgaagatcg
    19621 gcgaacgcaa gcgctggtac gaccttccgg aggaacgcgc atgaggctct tcttcgccac
    19681 tgacatccac gggtccgaga cgtgctggcg gaagttcctg aacagcgggc tgcattacga
    19741 ggcggacgta atcgtcctcg gaggcgacat gaccgggaag gcgctcgttc ccgtcgtcga
    19801 cgaaggcgac ggccgctggc acgccaccct gctcgaaaac cgcgaagagc tcgtcggcga
    19861 agacgaggtc gtggccttcg aggaagccgt gattcggcgc ggttactacc cgttcaggac
    19921 cacatccgaa gaggtgcgcg agctgcaaga cgacgaggcg cgttggcacg ggctgttcga
    19981 ggacgagatg ctgaagaccg tggagcggtg gatgcaggtg gcggatgaga agcttgccgg
    20041 caaaggcatc cgctgcttcg tctgtcccgg caacgatgat cagctcaacg tcgacgatgt
    20101 catccgcggc gcgaagagcg tcgagctcgc cgagggagag gtggtcgaga tcggcgggta
    20161 ccagatggtc tcgagcggct gggcgaaccg aacaccgtgg gatacctatc gagaggagga
    20221 tgaggaggac ctcgagatcc ggatccggaa gatcacggat caggtgacgg cccctcccga
    20281 gcggacgatc ttcagtttgc actgtcctcc gtacaagtcc gggctcgacg acgctccaca
    20341 actgacgaag gacatgcatc tgaaggaggc gggacgctcc actgtcccgg tcggctcgac
    20401 cgcggtgcgc gcagcgatcg aagagcatca gccctgcctc ggcctccacg gtcacatcca
    20461 cgaggcgaag ggcaccacgc gcatcggccg aactctctgc atcaacccgg ggagctcgta
    20521 cgagcaaggc cagctgctcg gtgccgtcgt cgatctcgac ggcaagaaga aggtcaagcg
    20581 gttcatcctc acgagcggat aggaggaaca cgaatggcaa cgatgtccgg gccagctcaa
    20641 gcaggagagc aagccctttt cgtccgcaag gcgaccggcc tcgtcaaagg ctggtcggtc
    20701 cgcgacgcgt tcatctactc ggcgatctcg atcaacttcg tcacgctggg cctgtacgcg
    20761 ttctccttcg cgaacttcat tcccaggggc agcctgttct gggcggcgat cctctcgggt
    20821 gcgtacctga tcttccaggc gatcacctac gcctcgctga ttgcggcgat gccgcgcgcc
    20881 ggcggcgact acgtctggat cagccggacg ctcggcggcg ggatcgggtt cgtgctggcc
    20941 gcttgcggat ggtggttcat cctctggcac tgggtcccga tctacgcgaa catcctcaac
    21001 atcatggtgc tgggcccgct ctcggccatc gtcggctggg atcgcggagt cacgttctgg
    21061 tcgagccaca acggcgtttt cacctcgtcg atgatcgtcg cgctgatggc gtcggtgctg
    21121 gttgcggccg gggtccgggc gtacgcacgc ttccagaaga tctgcttcta cggcggctgc
    21181 gtgggcctcc tgatcatgtt catcctcctt gtcgtacatt cgaagagcga cttcatctcc
    21241 gcgttcaacg cgcaggcgca cgacgtctac cacacctccg gggacgcgta cgcggcgacg
    21301 gcgaaggcgg gcagcgcggg gacctacgca tcactcgggt cattccactt cggccagatc
    21361 gtgctgttga ttccgatcct cttcttcttc aatctctggt cgaactgggg tgcgactctg
    21421 tacggcgagg tccggggagc gtcggacttc cgcaagaaca tctacgcgat gggcggggct
    21481 ctgctcgcga cgaccgcggt cgcggcgatc ttcttcggcc tgtttgcaca cacgttcggc
    21541 tggcagtggt atcacgcgtc gaacgccgcc tactgggccg gtaccgggcc gctcggcgtc
    21601 tttccctacc caggaacgct ggttgccttc ttcaccggga gcgctgcgct ccagttcatc
    21661 atcgtcggca tcctgagcct ctggttcttc ggctgggtcg ggacggtctt cctgtcgtcg
    21721 acccgcgtcg tcttcgctgc ggcgttcgac cgcgtcctgc ccgaggccgc ggcgaaggtc
    21781 agtgccaagg gcgtcccgta cggtgcgctg gcgttgatgc tgatcccgtc gattccgatc
    21841 agctacttct acgcgtacaa cacgaagttc agcagttgga cgttcgacgc aacgttggtg
    21901 atcgcgatca cgttcctcgg ctcggcggtc tcggcggcga tcttcccgtg gcgccggccg
    21961 gacatctaca acgcctcgcc gatcgcgaag tacaaggtgg ccggcattcc gctgatcacc
    22021 gcggcggcga cggccttcac ggcgatcctc gtgttcgccc tctaccactg gttcaaggac
    22081 tccgtctacg gcgtcaactt ccggaactcg gagtactaca tggcggtgct ctatggcgtt
    22141 gcgatcgcgg tctacgtcgt gtcgtggctc taccgacgct cgcaagggtt gagtctgaag
    22201 atggtctatg gagagatacc ggctgagtag ccggcgaaac ctggggagcg cctcgggcga
    22261 acagctgcga ggcgcttcct cgtctgtcag agctcgaggc cgaacgacga cgagcgatgg
    22321 cgccccacga ccgtgcgata ggctcgccgc cccgtgagcg tagaagtcga gacagtccga
    22381 acggtcgaag tgccgactcg gatcgtccat ggtgccggcg cgatcgcgcg cgtgggcgag
    22441 ctcgtcgcgg agctgggagt cagtcggccg cttctggtca cggacaaagg agtcgtcgcg
    22501 gccggtctcg ccgatgcgac gcttgcgcac ctcgagaacg cggtcctctt cgacgaggtc
    22561 cgcgcaaacc cggacatcga gctcgtcggg cgcgcccgga ccgcgtacgt cgaagccggc
    22621 tgcgacggcc tcatcgccct cggcggcggg agctcgatgg acacggcgaa gtcggttggc
    22681 gtcgaggtcg tccacggcgg ctcgattgtc gagtacgagt acggcgggac gccgatcacg
    22741 acgcgcatcc cgccgcttgt cgcggtgccc acgacagcgg gaaccgggag cgaggccacg
    22801 ctctgggccg tgatcacgga tccggagcga aagatcaagt tcaacgtcgg cggcacgccg
    22861 ttgatcggcg cgcacgtcgc gctcgtcgac cccgagctga tgctcggcct tccgccggcg
    22921 atcaccgcgg cgaccgggat ggatgcactc tcgcacgcga tcgagtgcta cacgtgtgac
    22981 taccaccaac ccttcaacga cgccgttgcg ctcctggcga ccgagctcgt cgcacgctcg
    23041 ctgcgcgtcg cctacgaaga cggcggcgat ctcgaggcgc gcacggacat ggcgcacgcc
    23101 gcgacactcg gcggacttgc ctacgggacc gagagcgcgg gcgcagccca tgccatgagc
    23161 cagtcggcag gcggcgtgca cgagtgtccg cacggcgcgc tcacggcacg agtgctcgga
    23221 cctgtgtgcg agtacaacgc gcccgcggac ccggagcgct acgcccgcat cgcgcagggt
    23281 ctcggggtcg acgtccacgg actgtctcca ctcgaagccg cgcttgccgg ggtcgatgag
    23341 gtctaccggc tcactgacga cgtcggcatt ccgagcatgg cagagctcgg cttctcggag
    23401 gaagagattc cgatgcttgc cagaatcgcc tacgaggatc cgcagacgat cggaaaccca
    23461 cgcgaggtcg acgccaccgc ctacgaagag atctatcgca actccttcgc gagagggaaa
    23521 cgctgagcac gcgcacgagc gactactcga tgtacgtcgg cggctcgtgg gtcgaaagcg
    23581 agtccggtga acggatggag gcgaccagtc cggcaacagg tgagacgatc gggtccgtcc
    23641 ccgaggcgac gcgcgacgac gctcggcggg cgatcgcggc ggccaacgcg gccgcgcgcg
    23701 agtgggcggc ccgctcggct ttcgagcgcg cggcggcaat ggagcgagta gccgagatca
    23761 tcgaggagcg ccgtgatgac cttgcgcgca cgctcacgct cgaccagggc aagcccctgc
    23821 gcgccgagtc ctacggcgag gtggatgagc tcatcgagta ctggcgaatg gccgcaggcg
    23881 atgcgcgacg gctcgaaggt ctcatgccgc cgtcggtgga cgcgtcgaag cgggtgctcg
    23941 cctaccgcgt tccgcgcggg gtcgtcggcg tgatcacgcc gtggaactgg ccgtacacga
    24001 tggctgcgga ggtagtcgct ccggcgctcg cctcgggaaa caccgtcgtc tgggggccgg
    24061 cgacttcgac gtcggtgtgc tcggtcaagc tggccgagtg cgtcgtggac gccgagcttc
    24121 ccgccggggt cttcaacctg gtgaccggcc ccggctcggt cgtcggagac gagattgcaa
    24181 caaacgctgg cacgcacgca atcggcttcg tcggctcggt cgccacgggg taccgcgtcg
    24241 ccgagcgcgc cgcgggcaag gcgcttctgc tcgagatggg agggaacggt cccctggtcg
    24301 tcctcgagga cgcggacctg gacggcgccg tcgaggcgac gctcgcggct gcgttcctgt
    24361 gcgccggaca gagctgcacc gccggcgagc gcttcctcgt gcacgaggac gtccacgacg
    24421 agtacctcga caagctgacg gcagcggtcg cgaaaacgat ccgactgggc gacccctttg
    24481 cggacgagac gacgatgggc ccgctcaaca acgagcgcac cgccgcgaag atggacaacc
    24541 acgtcggcga cgcggtcgag cgcggcgcgg tgctcgtcgc cggcggcgcg cgcgcgcgcg
    24601 agtttcccac ggacctgtac tacgaggcga ccgtcctcga ccgtgtgaac gacgagatgg
    24661 atgtcgccaa agacgagacg ttcgggccgg tcgttcccat ttcgacgatc cggagcgagg
    24721 acgaggcact cgcgatcatc gactcgtccc cgtacgggct gctggccgcc gtcttcacga
    24781 acgaccttcg ccgcggcctg cggttcgccg aggctgcgcg gaccggctgg gtgaacatca
    24841 acgagtcgac gaactactgg gagagccatc tcccgttcgg agggcgggcc gggtcgcaaa
    24901 gcgggatcgg ccgggtcggc ggacgcttct cgatggagac gttcacggag ctcaagaccg
    24961 tagtcgtgaa tctgggctaa ggaagaggag gcagctgcag tgacgaagat cgtcgagcac
    25021 cccgaggtct tgctgtacac gcgggtccgg aagtcgccgt acttttacgc gtcgcgccgt
    25081 cacggcgtgc acatgtacag cttctacaac cacatgtacc accctcgtca ctacggcgac
    25141 ccggtggcgg agtactggga acttctgaac ggtgtcacgc tctgggacgt cggggtcgag
    25201 cggcaggtgg agatcacggg gcccgacgcg ttcacgctgg cgaacatgct cgtcccgcgc
    25261 gacctgaaca agtgtgcggt cgggcagtgc aagtacgttt tcatcactgc tcccgacggc
    25321 ggcatcatca acgacccgat cctgctgcgg ctggaagaga accacttctg gatctccgtc
    25381 gcggacagcg atgtgctcct gtgggcgatg ggggtcgcct acaattcggg gctcgacgtc
    25441 aagatcggcg agccggatgt cggcccgatg cagatccagg gcccgaagtc gagggacgtg
    25501 ctcgtcgacc tccttggcga cagcgtgctc gagattccgt actacttcct gaaggattac
    25561 gagctcgagg gcatgcactt gacggtctcg cgcacgggct acacctccga gatcgggttc
    25621 gagatctact gccgcgatgc ggccaacaac ggcatcaagc tctgggacct ggtgctcgag
    25681 gcgggcaagc cgcacggcct cgccgttatc gggccctgcc acatccgccg catcgagggc
    25741 ggcatcctgg cgtacggcgc ggacatgtgg ctggatacga acccctacga ggtcgacctg
    25801 ggctacgagt ggatggtcga cctcgagcag gaggcggact tcgtggggaa ggacgcgctg
    25861 cggaagatca aggagaaagg aatctcgcga aagctcgtgg gcgttgagat cggcggcgcc
    25921 aagctcggct cctacaacga cggctcgatg atggacttct tccctgtgtc cgccgatggg
    25981 aagaacatcg gcaaggtgac gtcggcatgc ttctcgccgc gcctcgagaa gaacatcggc
    26041 tacgcgatgg ttccgatcga gtacgccgag ctggggacgg agctcgaggt cgagaggccg
    26101 gaagagaaga cctcggccgt tgtcgtccgc aggccgttca tcgatcctga gaaggagatt
    26161 ccgaagcagg acctcgcggc cgcgggggcc agggacgaat cgagcagcta acggaaccaa
    26221 tgccggtcgg gacgcgctcg ccgcgctact gaggcgtcct cgctacgagg tgttccccgt
    26281 cgaggggatc gccgacgacg tggtcacgca cgtcccgacc gagatcaagg tggcggtcac
    26341 ctcgtcgccg gcacggggga tcgaagcgac gctgcgactg tgcgaggagc tcacgtcggc
    26401 ccagttccag gttgcgccgc acctcgcagc tcgcctcgtc agggacgagg agcatctgca
    26461 agacatcctt cagcggctct tcgcgatcgg cgtccgcgac gtcttcgcga tcgccggcga
    26521 cgcggacctg cctgcaggga agttcgacgg cgcgacggcg ctgctcgtgg cgatggccga
    26581 gctcgggcac ggcctggaac aggttgggat ctccggctac ccggagagcc atcccttcat
    26641 cagcgacgag accacgattc aggtgatgtt cgacaaggag cccttcgcga cgtacatcgt
    26701 cagccagctc tgcctcgacg ccggtgtgat cgcgtggtgg atcggagccg tcagagcccg
    26761 cggcgtccgg ctccccatcc acatcgggat tcccgggccg attgcgagca cgaagctcct
    26821 tcgcatcgcg acgaggatcg gagtcggtga gtcgacgcga tttctgcgcc ggcacggcgg
    26881 gctgctcgca cgcctgttcc tgcgcggccg ctacggcccc ggctcgctga tcgagcagtt
    26941 ggcgccgcag ctcgccgacc cagcgtccgc aatcggcggc ttccatgtct acacgttcaa
    27001 tgaactggcg acgacggaac gctggcggcg cgaggcgctt cgccgcctcg agtcctgaag
    27061 agctcatcga cacgccggac gacgttttcg ccggcgagac cgcgaagggc aagcgtcgtc
    27121 cggcgccgga gcacgtcctc cggcccgtac gcccactcga actcgcgcgc gtacaaggcc
    27181 tgcgcgccga tgtcgggccc ttttgggtga atcggttgga gcaggtcagg atccgcgacg
    27241 gccggagcaa gtacctcgtc ggccaggctc ccgtacagat ggacgaggtg cgtgcagacc
    27301 gccacgtcga ggtcaggcaa gcgacgggca agccggcgca cgccctcgac cgggtcgatc
    27361 gcgcccggta gggggaaagg cccccgatcg agacggtgca agccgaactc ggcgcgaagc
    27421 gtcgtgagcg ctgcgtgcgc gatccggcgg taggtcgtga gcttgccgcc cgccacgctc
    27481 agcatcccgg tcgggccggt gaggaagacg gtctcgcggc gggcgctcgt cgtggcgccg
    27541 tcgcccatcg ggaggacgcg caggccggcg aaggtggagc agatcgcttc cggccggagc
    27601 aggtccgcct cgatcccgac ggccgcctcg ccgagtatct gcgcgacatc ggcttcggtc
    27661 gcctggactt cctcgggccc tcggtcgaag agcgtgtccg tcgtcccgag caggagcatg
    27721 ccctgccagg ggacggcgaa ggagacgcgc accttgtcgt gggggatcgt gagcgcggcc
    27781 gcccacggct cgtcgagggg cagcagcacg tgcgcgccct tgctgagccg gaccgatggg
    27841 gtcgccgcgg ggtcctcgag ccggcgtacc tcgtcaaccc acgggccggt cgcgttgacg
    27901 accacgcgtg cggaaaccga gacttcggtt ccgtcgacga gtacctcggc cccggcgacg
    27961 cgaccgcgaa cgtttcgcag cccgcgaacc tcggcgtagt tgagcaccgt cgcacctgcc
    28021 tcagccgcag cccggacgtt tgcgaggcag aggcgcccat cgtgcgtggc tgcgtcggcg
    28081 tagaggccgc acgagcgcag cccgtcgagg cgcaggctcg ggatgtgctc ccgtgcgcgc
    28141 tcggggggca cgagccggtt caatcgtgcg cgggcgagcc cgctgtagag gacgagaccg
    28201 gtttgaatcg tcaggcgccc gtaagggcca ttgcggtaca gggggagcag gaagggcaga
    28261 cggcggacga gatgcggcgc gacgacctgc atcagtagcc gccgctcgtg gtgggcctcg
    28321 cgaacgaggc ggatgtcgcc gagccggagg tagcggaggc cgccgtggat gagtttcgag
    28381 gaagcgctcg aggtgcccgc gccaaagtcg cggcggtcga cgagcgcgac ggcgaggcct
    28441 gcgcgcgcgg cctcagccgc gattccagcc ccgacgatcc cgcccccgat cacaagcagg
    28501 tcgaacgtcc gttgcccgag ccgctcgcgc gcttcctcgc gggaaactag gtccacaacg
    28561 ggttcgtcca ggccttccgg ccgtaggagt cgacaagctc gagccgtccg tagaggctgc
    28621 cggccggcca ttcgaggcgt gcgcacgtga tctcttcgtc gtctgcctcg gacaggatct
    28681 cgccggcgta ccggtagccg aaacggccgg cgctgacgga ggagccccgg cgggggctgg
    28741 tgcacagcgt gactctctga cagggactgc acagcacctc gacgctcccc ttctcgaggt
    28801 ggagct
//
LOCUS       DASVEU010000118        27000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_660764,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000118 DASVEU010000000
VERSION     DASVEU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 27000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27000
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660764"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..604
                     /locus_tag="VF225_04755"
     CDS             194..604
                     /locus_tag="VF225_04755"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7144598.1"
                     /translation="MTVVAKEFRYTLELDRTGRVTADGGSPLDLDTAWTPEHLVLAGL
                     VRCTLQSLRFHAAPLGIDFLASASASAMVTRRPGDGRYAFVEIDVELELELEPTPPGE
                     GLAALLAKAERDCFVGASLNPSPRYHWRVNGIAL"
     gene            complement(631..939)
                     /locus_tag="VF225_04760"
     CDS             complement(631..939)
                     /locus_tag="VF225_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144599.1"
                     /translation="MVEIAPRYDRRLLEAVQALDDRAESMAEISRRVGVAAAQFGLPK
                     PSYVHLRRYIVAHREEEDTERARRDEIRQILYDAHYDFMRSRVVDAYDIAERIREAGR
                     "
     gene            complement(1031..1318)
                     /locus_tag="VF225_04765"
     CDS             complement(1031..1318)
                     /locus_tag="VF225_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX7144600.1"
                     /translation="MAPATKERIDRGTGTGLGAPWNVIVLNDNHNTFQGVASALASTL
                     PEVSYDQGLRFADRIHSSGRAIVWSGHKEVAEHYWQQLHGHGLTMSPLERS"
     gene            1346..2509
                     /locus_tag="VF225_04770"
     CDS             1346..2509
                     /locus_tag="VF225_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX7144601.1"
                     /translation="MSTVTYDVEAVRARYSALRQPLAFFDGPGGTQVPDEVIDAVSRY
                     YQESNANVSGPYETSRRTEALVDQARVTAGRFLGCTSEEAIFGANMSTLAFALTRTAG
                     RDWREGDEILVTKLDHDANVSPWLELAHDKELVVRFADINDDTTLDLDDLERQLSDRT
                     RVVAFPVASNAVGTLTDVQRIVELAHDAGALAWADAVHYGPHGPIDVTDWGVDVLICS
                     PYKFFGPHLGLAFGRAELLQTWRPYKVRPAPDLPLGRRFETGTLAHELMAGFVAAVEY
                     IESIGWESIKAHERTLGERFLTGLPENATLYGLPTMEGRVATFAFNVGGVSTREAATR
                     LGDAGYAVWEGDYYAVEVMKRLGLQQSGAVRAGIVHYNTADEVDGLLGEISTL"
     gene            complement(2506..3372)
                     /locus_tag="VF225_04775"
     CDS             complement(2506..3372)
                     /locus_tag="VF225_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7144602.1"
                     /translation="MLNALTIILLFAAAWAVSRLSAIVARKVMAWSDRRHRTPSGDLS
                     AKIVELKRRETSVAVIRTGIAYIAFATALVLTAAQLTGGFGRLTAVAGASFILILVGF
                     SAQRMLMDIIAGFNMFVEHWYSVGDTIAIPMLEVQGIVEDLSLRRTKLRSLDGEVINI
                     HNSQIAAVRVLPGGVKEFDIEVVASSREAAELLVEHVGAILPEGPTTFIRRPVIHQID
                     DLAPGLVRLRMRAAVAPGREWLVHGFYTDLLKERADKHLIVHGPVVLTVDDNVERSFA
                     RASAATRWAGAR"
     gene            3462..4754
                     /locus_tag="VF225_04780"
     CDS             3462..4754
                     /locus_tag="VF225_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7144603.1"
                     /translation="MTPVELLQQLIRFDTTNPPGNEAECVGLLRSLLEEAGCETNTYA
                     KDPARPNLVARLQGSGDSAPLLLQGHVDVVTTAGQNWERRPFGGDLVDGEVWGRGAID
                     MKGAVAMFVSAFVRAARGELQLPGDVILVVLSDEENGGDFGAKFLVEEHPELFEGVRH
                     ALGEAGGISQVIAGKRFYPIQLGEKQICWLKATVRGPGGHGAMIHRDGTMARLGRMLD
                     DLNRKRLPIHVTPIVCDMIETIAAHLPRPKRDLMLALLKPAVADRALPRLGPRLRWLE
                     TLLRNTVNATIVHGGDKVNVIPAQIDLELDGRLLPGFTPDDLIGELHGIVGDDIEVEL
                     VRHDPGLAEPDLALFEMLGGVLRELDPEGIPMPLLQGGVTDGRFFAQLGIQTYGFMPM
                     RLPADFPVLKIVHAADERIPVAALEFGTEAVTRALQRF"
     gene            4754..5074
                     /locus_tag="VF225_04785"
     CDS             4754..5074
                     /locus_tag="VF225_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144604.1"
                     /translation="MSDKDYSGTPLGKKLGARPGVEVLVLFTTNRAELERRFATLKKT
                     LAPADGLWIAWPKKASKIETDLDFPTVQETGLAAGLVDNKSAAIDGEWQAVRFVYRKK
                     DRAR"
     gene            5088..6356
                     /locus_tag="VF225_04790"
     CDS             5088..6356
                     /locus_tag="VF225_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7144605.1"
                     /translation="MSAFSLERLDNGLRILTAPMDSAQSVTCAIMLAAGSRYETPETN
                     GIAHFAEHMFFKGTERRPTARDISMEIDGIGGEFNAFTGKEYTGYYVKCAAESRDIAL
                     DVLVDMLRHSKFDPEEIEREKGVIIEEMNMYYDTPRDFIGGVYEELLYDDQPLGWEII
                     GRKETIRNATREMFGGYVDHWYQPSRMVVGVGGKIGDDLNERVQELLGDVAKAETGRP
                     EPIEVDPHDHPHVKVHTKASDQAHICLGVHSYPLEHPDRYVLQVLATVLGGGMSSRLF
                     TEVRERRGLAYYVYGLNHSYTDAGSLFTQAGVDINRIDDAVSTVAAELRKIAEEPVPA
                     AELEKAKSFAKGRFVLQLETSQGLIMFGLRREVLESRTPDPEEIIGELDKVTAEDVAR
                     VAGDVIEKQGFNLAVIGPFDDPDRFEKLLA"
     gene            complement(6719..8671)
                     /gene="acs"
                     /locus_tag="VF225_04795"
     CDS             complement(6719..8671)
                     /gene="acs"
                     /locus_tag="VF225_04795"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7144606.1"
                     /translation="MTNTIDTMLLEERRYAPDPEFARQANAQPEIYERDWEEFWETEG
                     RDRVTWFEPFSKLYEWEAPYAKWYLGGRLNVCFNCVDRHVEAGNGEKVAYFWEGEPED
                     ERRTITFADLQREVVRFANALKKLGVAKGTPVGIYMGMVPELPIAMLACARLGAPHTV
                     VFGGFSSESLSDRMNDMECALLITQDEGWRAGKKVPLKQNADEAVAQAPTVKNVVVLK
                     RTGDQVPFDASRDAWWHELVEGEDDDPASAPPEPMDAEDLLYLLYTSGTTAKPKGIVH
                     TTAGYLVGVAATHHYIFDVKPDSVYWCAADVGWVTGHSYIVYGPLANRTTGVMYEGVP
                     NYPNPERWWQIIERYKVDILYTAPTAIRSHMKWGPEHAQKHDLSSLRLLGSVGEPINP
                     EAWIWYREHIGGDRTPIVDTWWQTETGMILITPLPGVTTTKPGSATKPFPGVDAGVYS
                     ESGDEVGPGGGGYLVLKRPWPAMLRGIYKDDQRFRDTYWSKYDDIYFAGDGARIDEDG
                     DFWLLGRVDDVMNVSGHRISTIEVESALVDHKKVAEAAVCGRKDDRTGQAIVAYVTLK
                     GGEEGSIEMLEELRNHVAKKIGPIAKPANIVFTPELPKTRSGKIMRRLLRDVAEQRTL
                     GDTTTLADPTVVEEIKERAATDAGED"
     gene            complement(8668..9804)
                     /locus_tag="VF225_04800"
     CDS             complement(8668..9804)
                     /locus_tag="VF225_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7144607.1"
                     /translation="MPDVLIYGDTIRSPELRHEVPVPIPDPFLYAEKGNRRIVVLHSL
                     EIPRVREDAPDLDIVPLEDLGSDELFTQGLQGWKVVLELAVRACRELGIERATVPESF
                     PVGHADHLRTNGVELVVDRDLFDNRRRSKNETELRGIRNAQRACEAALDASRELLRRA
                     QPNGAGLEVDGEPLTCERLKRIIEDVFADHEVEGSDMIVSHGSQTAVGHNMGSGQIGP
                     DEPIVFDLFPRDKATGCYADMTRTYVVGEPSDEVKEWHRLVKEALDTSTAGVKPGVNG
                     RKLYEEVCDIFQSAGYKTQLNKEPGEVLEDGFFHGLGHGVGLEVHELPSMSRIGHDLV
                     PGDVVTIEPGLYRSGYGGLRLEDLVLVTNDGHEVLTDYPYDLAP"
     gene            9869..11023
                     /locus_tag="VF225_04805"
     CDS             9869..11023
                     /locus_tag="VF225_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7144608.1"
                     /translation="MDDAPQRAQKLMAQWDDELRKELPAGADIFDAHTHLGTDIDGMV
                     GIYEDLERGMDKYGISRCFMFCLDEPDRHPGFRAGNDRTLEFGGRSRGRMIPFVRLDL
                     AEDPIGEATRMLDLGAKGIKLHPRAQKFLLNDERLSPIFELAAERKVPILIHGGRGLP
                     PIADDLARVVDRYPDAQLIVAHAGIADLAALADRLGGKAGVFFDTSVWSPLDLLSLYR
                     LVGPEQVVYASDYPYGQQPASLLISLRTARLAQLTDEQVRGVLAGNANRMADGGAPVE
                     PSAPKGLETFSQPMSLARIHQYLSMATPLLWTRQQDRIGVLGLALNACDDRANGHREE
                     LDQIRELLTVAQDMWLTLPEDGDERDRMAHTRATFRLIHLADIVAVTTPA"
     gene            11031..11453
                     /locus_tag="VF225_04810"
     CDS             11031..11453
                     /locus_tag="VF225_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7144609.1"
                     /translation="MNGETHSFDAPVEQSLAQVLREDLGLTGTKVACGEGHCGACTVQ
                     LDGVPTLSCITLVHTVGNREVTTIEGLRDHPLIHAFVRTDAVQCGFCTPGQVVSAAAL
                     VEGNPNPSTDEIRHAMSGNLCRCGTYPKIEEAIRTWQD"
     gene            11441..13705
                     /locus_tag="VF225_04815"
     CDS             11441..13705
                     /locus_tag="VF225_04815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7144610.1"
                     /translation="MARLIRTEKEVEGRYEEVWIVVDEDALDQWPAGPLTTVGRRVPR
                     VDGLARARGQAQFTADLQLPGMLHTAVLRSPYARARVTSLDLEPARSAPGVRAAIGPA
                     DEDQLTAEPSYVGHAVAAVAADTFSQAQAAVERIVVDWEVLEPLLDPDEAVRQESFVS
                     DVARYERGDFERGLGEADVVVEAEYRTQVVVHNSLETHQAVCHWEDEGITIYISTQYI
                     WGIRAAMAAHLGLPPDKVRVICQAMGGGFGAKNSPGDYTPIAAELAKRTGRPVRCALT
                     RREENVAAGNRNATIQRLTAGATSDGTLVALGGDFVNAVGFSGWSATTEGPMKMLYSC
                     DNVRTTQYPAKLNLPPMAAFRAPGFVEGTFALECLLDELAVKLGVDPLELRRRNYADS
                     DLTDDRPFSSKNLAECYRRAEPHWAKRDEVRARSQGPWKHGIGLASQIWYGGGGPPSY
                     AWIRVGSDGRAQVVTAMQDIGTGTRTAMAQIAAEELGVPLGHVQVSLGDSARGPFASI
                     SAGSSTVPSMGPAVRSAAADAKRQIIEIAAQRFHLEERVLDLREGRIVSADGGSWPLT
                     EVTGLLESGQILGKGARGPNPAGMQVLTFGVQVAEVAVDVETGEVRVDKIVAIHDVGR
                     VINALGAESQVEGGIIQGIGHTLSEERIVDPATGSVLTRTLDAYRMPTIADVPEIVTE
                     LVDIPDEHLTNLGSKGLGEPPIIPVAAAIANAIRDATGAEIRSLPITREEMFRALREA
                     DAREKERRGAAAAV"
     gene            13683..14531
                     /locus_tag="VF225_04820"
     CDS             13683..14531
                     /locus_tag="VF225_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7144611.1"
                     /translation="MELLRPSSAEEATGALGNGSRALAGGTELVPLMRDGLLKAETLV
                     DVRGVVPRGIAGNRIGAGTTLAELELDAQIPQALREACAQAASPQLRSMGTIGGNLLQ
                     ATRCWYWRLGFDCWLHGGEKCFAKDGAQREHAIFGNEQCASAHPSDVAAALLALDATL
                     RTTRREFAVAELYRLPTEENRNVTTLAPDELVLEIELTTPDASTYLKAMDRRKWAFPI
                     VGVAAARFGSETRFALAGVAPIPWLLDSAAALDDATPLPGTAYKIEIARALVRRATHA
                     VKSASR"
     gene            14542..15201
                     /locus_tag="VF225_04825"
     CDS             14542..15201
                     /locus_tag="VF225_04825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7144612.1"
                     /translation="MTTFRREISERGWGPRRAKPTLAAVFGLALFALAGCGGGTKQQA
                     ATTCPTTGKQVTFPLLDKSKASTVGVLTNFGRFDIRLDVRDSPCTTSSFAALVRKGYF
                     DGTRFHRIVTGFVIQGGDPTATGQGGPGYTVQDVPPANATYTRGVVAMAKTNAEPPGT
                     SGSQFFVVTGADVGLPPQYAILGTVTKGLKVIDRIGKLGNPANERPTRKVVVQRMTVT
                     P"
     gene            15212..15712
                     /locus_tag="VF225_04830"
     CDS             15212..15712
                     /locus_tag="VF225_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7144613.1"
                     /translation="MIAAVVLAAGASTRFGSPKQALLLEPVLERVRSSATIGNTIVVL
                     GAHDVETNARVVRCPEWHRGPGASLRCGLDALGEEIEAAVVVLADGPSLDPRAIDRVV
                     AAWRESGAERVAATYGGIRLHPLLLAREAWREVPDQGLRGLAAELVPCDDLDPPGDVD
                     FADDLS"
     gene            15752..16435
                     /locus_tag="VF225_04835"
     CDS             15752..16435
                     /locus_tag="VF225_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX7144614.1"
                     /translation="MELVHPLEPTGEESPLARARLHRQLTVEETARRAGITAEEVRWL
                     EEGRVYRFPKPDDALIATALYATALGLDNREARELAGLPVPPQPIKRNPLPRLLVVGG
                     VVIALVVFATLLLIPRSGGKPKQGSPSAILPPPWRIPVNVLNGSGDINYTRQVASRIG
                     AFGYTIKKVGRADNFTYPQTAVYFPPKCEGVAFRLAKQLGVPTKPLPGGTGVCQLYVI
                     VGPARGPGE"
     gene            complement(16442..16678)
                     /locus_tag="VF225_04840"
     CDS             complement(16442..16678)
                     /locus_tag="VF225_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7144615.1"
                     /translation="MTAYDPCAHCEEMMQPYMDRTITDAERAEAEQHLDGCTYCRKRY
                     RFEEKLRQFVRQAVQQEPMPVELKTKLAGLRTPL"
     gene            complement(16675..17268)
                     /locus_tag="VF225_04845"
     CDS             complement(16675..17268)
                     /locus_tag="VF225_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144616.1"
                     /translation="MSETTREPDARQLATEARDRVRFEEDALALSDQVYRVARHLVGS
                     REEAEDLMQETYARAFRSWRSFTPGTNLRAWLLRILTNLNIDRGRKQQRTPDTQPLEE
                     GDYFLYNKLEASGDGASDEDRVVERLSQNDVVGALSEVPHDFRDVVVLVDIGDFTYAD
                     AAQILDIPIGTVMSRLHRGRRILKQAMAESAVGGEEA"
     gene            complement(17337..17549)
                     /locus_tag="VF225_04850"
     CDS             complement(17337..17549)
                     /locus_tag="VF225_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX7144617.1"
                     /translation="MAVVTETIQMSGIRCERCVMRLAKVLEGHEGLEEANANLMGQVT
                     LAWDDERTKRDSLLEAMARGGFREVP"
     gene            complement(17554..18609)
                     /locus_tag="VF225_04855"
     CDS             complement(17554..18609)
                     /locus_tag="VF225_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX7144618.1"
                     /translation="MIEQQFGPSLTVGVEEELWIVDAETLQLVPAVETLVAGAEGRNL
                     PGTLKTELHASVVELTTDVSDTAEQAQERLVELRRAARDIAAQNGYVVAAGGSYPTSI
                     PSEQKIAPVERYRDFVEYAGPSARRQGVSGLHIHIGMPDAETCFRVMETILPWLPLVL
                     ALSANSPYFEGRDTGMLSIRAEVLGFLPRHGAPPAFRNYAEWERFIERMAATGLANDY
                     TSFWWDVRPHPRFGTLEVRAPDQATSVEHTAALVGLVHALCAWALEAPPRPFEPAERG
                     VYEQNRWAASRFGPHGKLVHPDRDEALTVAALARELPVRLHGLDPETCEADRQLEIGR
                     AEGLSAVCADLVERSVA"
     gene            complement(18606..18968)
                     /locus_tag="VF225_04860"
     CDS             complement(18606..18968)
                     /locus_tag="VF225_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144619.1"
                     /translation="MEEGRMEPGHGVLADAELVWEEELWRDDEAGDRSGWFCVRTNPF
                     PCPAAGCTFVAGFMTAAHLILVWERRDDPNLLHHAQRAKEVGRNPRIQAYEPSYGPSA
                     SYYAWEAAGGPVHGVRAR"
     gene            complement(19098..19349)
                     /locus_tag="VF225_04865"
     CDS             complement(19098..19349)
                     /locus_tag="VF225_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7144620.1"
                     /translation="MSIGLAHLNCVELVELVTAYLEGDLSSGERKRFDAHLSACDGCT
                     TYVEQMRRTIELTGTLRVDDVSQDAEEALRHAFRDWRAH"
     gene            complement(19346..19966)
                     /locus_tag="VF225_04870"
     CDS             complement(19346..19966)
                     /locus_tag="VF225_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144621.1"
                     /translation="MGQFLSPQDERLVERLRTGDEDAFMELIRDLNPSLLRVARMFVP
                     TAAVAEDVVQETWLAVLKGIERFEGRSSLKTWIFRILTNTAKTRGERERRSVPFSALD
                     TEEGGFEPAVERSRFTGTGHWAVLPRAWPEDRLLAKETLSVIERAIERLPPSQQLVIT
                     LRDVEGWTADEVRNALELSETNQRVLLHRGRSKVRRALEQYLSEEQ"
     gene            complement(19997..20257)
                     /locus_tag="VF225_04875"
     CDS             complement(19997..20257)
                     /locus_tag="VF225_04875"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX7144622.1"
                     /translation="MEALRLDGNAAAGLLSEVFAGEVTTAIGTCDSCGSAEAVGAIHV
                     YMAAGTVLRCPHCEAVVMKVVTDGERIWVDVRGLRTLALAPI"
     gene            complement(20259..21005)
                     /locus_tag="VF225_04880"
     CDS             complement(20259..21005)
                     /locus_tag="VF225_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX7144623.1"
                     /translation="MEGTAVLGRLNWHVAEVADAWPETDRVKTLALDVQDWTGHRAGQ
                     HVDVRLTADDGYQAQRSYSIASPAGGPRVELTVERIDDGEVSPYLTQDAHAGDRFELR
                     GPVGGYFVWDPDPGGPVLLVGGGSGIVPLMAMVRQRATNHDAVPTRLLYSARSWEDVI
                     YRDELERYRKREDGLEIVFTLTRDQPPDWTGYSRRVDRQLLSEVAPADLALTFVCGPT
                     PFVEAVAEALVGLGHEPLRIKTERFGPTGG"
     gene            complement(20975..21580)
                     /locus_tag="VF225_04885"
     CDS             complement(20975..21580)
                     /locus_tag="VF225_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX7144624.1"
                     /translation="MPIISRGFKGRRRDDAPRDRVPPGQYVTDDFPVLSAGPTPHTPL
                     DQWDFTIGGEVDGQRRWTWDEFRALPSETITVDISCVTKWTKLDTVWEGVPVDALLEG
                     VETSAEYVTAFCDGGYTTNLPLADVTDGKAWVAFAYDGEPLEPEHGGPARLLVPHLYF
                     WKSAKWVRGLRLTETDEPGFWESNGYHIYGDPWKEQRYWGD"
     gene            21702..23081
                     /locus_tag="VF225_04890"
     CDS             21702..23081
                     /locus_tag="VF225_04890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7144625.1"
                     /translation="MNAERYDLIVLGSGSAARDGAGKAAREHGARVALIEHRLWGGSC
                     PNVACRPTKAYVVAAELMHDVRHHAAERGIDLPEPAVRLAQTRKWKDALRRDQRSWLE
                     TLKDAGYGVVPGTASFVDARTVRVGDQQLSGERILIATGSRTAVPPIPGIEDIVWIDH
                     VSALELDEVPESLLIVGAGPVGLEFAQIFARFGSRVAIVNSGPQIAARADTEAAGELQ
                     AALEEEGIEVVLSSGVDSLARSGTGVAAAVGGRTIHVSHVLLASGRLPNVEELDPARI
                     GLEIGRGGIAVDDRQRTNVGGVWAAGDVAAGPMLTPIAQYQARIAVEDMFGDGSRRAD
                     YSALPTAIFTDPELGSVGLTEAEAGEHGHDVGVVKHPLPSVTRAQYTGAKHGLYKIVF
                     DRETRRVLGVHVVSRAASDIVGSITPALKLGVTVDDLAFMHHVYPSYSEGLKAAAEKA
                     LVSTSEAKS"
     gene            complement(23253..24437)
                     /locus_tag="VF225_04895"
     CDS             complement(23253..24437)
                     /locus_tag="VF225_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7144626.1"
                     /translation="MHHFLHLRSRAAGLFAVALLGGGVALGGAAALGKLGEHTTVIRQ
                     EVVPSSSAPAAFAQGKRMSINEIYRASAPAVVHIETTSRAQPPSDFFGNPFGASQTQR
                     ALGSGFVIDKAGHIVTNYHVVRGANTIQVSFSDNERFKAKVVGVDPSTDSAVLQVHVK
                     SRALKSLPLGNSDAVRVGDQVIAIGNPFGLDRSVTAGIVSAVQRRISAPNQLSISHVI
                     QTDAALNHGNSGGPLLNAQGEVIGVNAQIETGGQSQGNVGIGFAIPINTVKDVVAELI
                     KHGKAEHAFLGIEGKTLQPNIGRLFHLPVTSGVLVASVRPRTGAAKAGLKPATNQVTV
                     EGESWPAGGDVIVKADGQPIPSIERLVDLIATKKPGDNIDLEVVRGTSRIHVNVKLGR
                     QP"
     gene            24671..25261
                     /locus_tag="VF225_04900"
     CDS             24671..25261
                     /locus_tag="VF225_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144627.1"
                     /translation="MRLLKRFTPSPAMVIACLALLLVLGGTGYAASKALPRNSVTSVQ
                     VKDHSLLARDFKAGQIPRGAAGPAGPAGPAGPAGPAGPAGTTGSASVKWALVRPDGGI
                     AAQSGGITLAAHPSTGTYILNIGSAITGKPILASGGYAGDAGDQRGETTAGPCGGGSE
                     GRTCPSGFDTTSHLFAQTRNNDGVVTDHAFYVVVFG"
     gene            25313..25771
                     /locus_tag="VF225_04905"
     CDS             25313..25771
                     /locus_tag="VF225_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7144628.1"
                     /translation="MRVSAKADYALRAVIELAVIGGDGPVKGERIAQAQEIPLKFLEN
                     ILGDLRHAGLVRSQRGVDGGYWLARPADEITVAEVIRGVEGPIANVRGVGPEQVEYAG
                     SAERLREVWIAVRASLRAVLEQVTIADLASGELPLSVEELASNPDAWQPH"
     gene            complement(25781..26734)
                     /locus_tag="VF225_04910"
     CDS             complement(25781..26734)
                     /locus_tag="VF225_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX7144629.1"
                     /translation="MIEARGIVKRFGAFTALDGVSVDVPTGSLTALLGPSGSGKSTLL
                     RVIAGLEQPDAGEVFLDGEDVTRRQPQARGVGFVFQHYAAFKHMTVWDNIAFGLTIRR
                     RPRTQIKERVSELLDLVQLSGLAKRYPAQLSGGQRQRMGLARALAVDPKVLLLDEPFG
                     ALDARVRKELRLWLRRLHDETHTTTVIVTHDQEEAMDVADRVVVMNAGRIEQTAPPRE
                     LYDNPANEFVMSFVGPVNRLGDTFIRPHDVELLLEPNGSTQEAMVQRLVHLGFEVRVE
                     LVRDDGETMSAQLTREQVEALELQHGQIVYVRPTRQTVFTD"
     gene            complement(26731..>27000)
                     /locus_tag="VF225_04915"
     CDS             complement(26731..>27000)
                     /locus_tag="VF225_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144630.1"
                     /translation="FRRVTWPAIRYATAYGVVLTTARALGEFGAVSVVSGRLVGQTES
                     LTLYVQDRYQAFDTTGAYAAAVLLALLAVATLLLMTRLKPKEGIQ"
BASE COUNT         4495 a         9120 c         9012 g         4373 t
ORIGIN      
        1 cgcagggcct cgccctcgac cgccagctcc gggctcgcgc gacgcggctc cagactcacg
       61 atccgaaccg agtcttccca gccgccgtaa cacctggcat agcactcagc ctcgctgagc
      121 ggccgcagac gtcgcatgac caaacattac gccgcgcccc ggacggttcc tgccacgctg
      181 gcaggatagg agcatgacgg tcgtggcgaa ggaattccgc tacacgctcg agctcgaccg
      241 cacgggccga gtgactgcgg acggtggctc tccgctcgac ctcgacaccg cctggactcc
      301 ggagcatctc gtcctcgccg gcctggttcg ctgcacgttg cagagcctgc gcttccacgc
      361 tgcaccgctg gggatcgact tcctcgcgag cgcttcggcg tcggcaatgg tgacccgtcg
      421 ccccggggac gggcgttacg ccttcgtcga gatcgacgtg gagctcgagc tggagctcga
      481 gccgacgccg cccggcgagg ggcttgcagc actgctggcc aaggcggagc gggactgctt
      541 cgtcggcgcg tcgctcaacc cgtctccccg gtaccactgg cgcgtgaacg gcatcgctct
      601 ctagcttgtg gctcagagcc acaagctgat ttatcgcccc gcctctcgga tgcgctccgc
      661 gatgtcgtac gcgtcgacga cccgactccg catgaagtcg tagtgggcgt cgtagaggat
      721 ctggcggatc tcgtcgcgcc gcgcgcgctc cgtgtcctct tcctcgcgat gggccacgat
      781 gtaccggcgc agatgcacgt agcttggctt cggcaagcca aattgtgccg ctgcgacgcc
      841 aacgcggcgc gagatctcag ccatggactc cgctcgatcg tcaagggcct ggacggcctc
      901 gagaaggcga cggtcatagc gcggagcgat ctcgaccacg cgagcagcat gcgctcgagc
      961 gcgtcgcgac tctgtggcga ttctgttccg aaagacggtt agtggctcag agccacaagc
     1021 tctgagttgg ctatgaccgt tcgagaggcg acatcgtcag gccatggccg tgcagctgct
     1081 gccagtagtg ctcggcgact tccttgtgac ctgaccagac gatcgcgcgg ccgctcgagt
     1141 ggatccggtc cgcgaagcgc aatccctgat cgtaggaaac ctcgggcaag gtcgaggcga
     1201 gcgcggacgc aacgccctgg aaggtgttgt ggttgtcgtt cagcacgatg acgttccacg
     1261 gtgcgccgag acccgttcca gtgccgcggt cgatccgctc tttggtcgcc ggggccacgc
     1321 accaatcgta gtaacgtcct cgccggtgtc caccgtcacg tacgacgtcg aggccgttcg
     1381 cgcgcgctat tcggccctac gccagccctt ggcgttcttc gacgggcccg gcggcactca
     1441 ggtgcccgac gaggtcatcg acgcggtctc ccgctactac caggagtcga acgcgaacgt
     1501 cagtggccct tacgagacga gccgccgaac ggaggcgctc gtcgaccagg cacgcgtgac
     1561 ggccggacgg ttcctcggct gcacatccga ggaggcgatc ttcggcgcca acatgtcgac
     1621 gctcgcgttc gcgctgacgc ggacggccgg acgtgactgg cgtgagggtg acgagatcct
     1681 cgtcacgaag ctcgaccacg atgccaacgt gtctccgtgg ctcgagctcg cccacgacaa
     1741 agagctcgtg gtgcggttcg cagacatcaa cgacgacacg acgctcgacc tcgacgacct
     1801 cgagcggcaa ctctcggacc gcacgcgcgt cgtcgctttc ccggtcgcct ccaacgcagt
     1861 cggcaccctc acggacgtgc agcgcatcgt cgagctcgca cacgacgccg gcgcgctcgc
     1921 ctgggccgac gccgtccact acggcccgca cgggccaatc gacgtgaccg actggggggt
     1981 ggacgtcctc atctgctcgc cctacaagtt cttcggccca catctcggtc ttgcctttgg
     2041 ccgtgcggag ctgctccaga cgtggcggcc ctacaaggtg cggcctgcgc ccgacctgcc
     2101 actcgggcgg cgcttcgaga ccggcacgct cgcccacgag ctcatggccg gcttcgtagc
     2161 cgccgtggaa tacatcgagt cgatcggctg ggagtcgatc aaggcgcacg agcgcacgct
     2221 cggcgagcgc ttcctcaccg gcctgccgga gaacgccact ttgtacggcc ttccgacaat
     2281 ggaaggccgc gtcgcgacct tcgcgttcaa cgtcgggggc gtgtccacac gggaagcggc
     2341 gacacgcctc ggcgacgcgg gctatgcggt ctgggagggc gactactacg cagtcgaggt
     2401 gatgaagcgg ctcggcctgc agcagagcgg tgccgtccgc gccggcatcg tccactacaa
     2461 caccgccgac gaggtcgacg gcctgctggg agagatctcg accctttagc gggcgccggc
     2521 ccagcgcgtc gccgcgctgg cgcgggcgaa gctgcgctcc acgttgtcgt cgaccgtcag
     2581 gacgaccggc ccgtggacga tcagatgctt gtcggcccgc tccttgagca ggtcggtgta
     2641 gaagccgtgc acgagccact cccgtcccgg cgctaccgct gcgcgcatgc ggaggcgcac
     2701 gagccccggg gcgaggtcgt cgatctggtg gatgaccggg cgccggatga acgtcgtcgg
     2761 cccttccggc aggatcgcgc cgacgtgctc gacgagcaac tcggcggcct cccggctact
     2821 ggcgacgacc tcgatgtcga attccttcac cccacctggc aggacgcgaa cagccgcgat
     2881 ctgcgagttg tggatgttga tcacctcgcc gtcgagcgaa cgaagcttcg tccgtcgcaa
     2941 cgacaggtcc tcgacgattc cctgaacctc caacatcggg atcgcgatcg tgtcgccgac
     3001 ggagtaccag tgctcgacga acatgttgaa gccggcgatg atgtccatga gcatccgctg
     3061 cgcggagaag ccgacaagga tgaggatgaa agaggcgccg gccacggcgg tgaggcggcc
     3121 gaacccgccg gtcagctggg cggccgtcag aacgagggcc gtcgcgaacg cgatgtaggc
     3181 gatgccggtc cggatcacgg cgacgctcgt ctcgcgccgc ttgagctcga cgatcttggc
     3241 gctcaggtct ccgctcgggg tgcggtgcct gcggtcggac caggccatca ccttccgggc
     3301 gacgatggcg ctcaggcggg acacggccca ggcggccgcg aaaaggagga tgatggtgag
     3361 ggcgttgagc atctgttaca aagtttcctt ccgttgaagg tatcgacagg gatctctctg
     3421 tcacaatccc ccagccaaat ctttaccgcg cctttacctc attgactcca gtcgagcttc
     3481 tccagcagct catccgcttc gacacgacga accctcccgg aaacgaggcg gagtgcgtcg
     3541 gactcctccg gagcctgctg gaagaggcgg gctgcgagac gaacacctac gcaaaggatc
     3601 ccgcccggcc gaacctcgtc gcacggttgc agggatccgg ggacagcgcg ccgctgctgt
     3661 tacagggcca cgtggacgtc gtgacgaccg ccggccagaa ctgggagagg cggcccttcg
     3721 gcggagacct cgtcgacggc gaggtctggg gtcggggtgc gatcgacatg aagggcgcgg
     3781 tcgcgatgtt cgtctccgcc ttcgtgcgcg cggcgcgagg agagctgcag cttcccggcg
     3841 acgtgattct cgtggtactc agcgacgagg agaacggggg cgatttcggg gcgaaatttc
     3901 tcgtcgagga gcaccccgag ctgttcgagg gcgtccgcca cgccctcggc gaggccggcg
     3961 ggatctcgca ggtcatcgca gggaagcgct tctatccgat ccagctgggc gagaagcaga
     4021 tctgctggct gaaggcgacc gtccgggggc cgggcggcca cggcgccatg atccaccgcg
     4081 acgggacaat ggccaggctc gggcggatgc tggacgacct gaaccgaaag aggctgccga
     4141 tccacgtcac gccgatcgtc tgcgacatga tcgagacgat cgccgcccat ctgccccggc
     4201 cgaagcgtga tctgatgctc gcgctgctca aaccggccgt cgccgaccgc gcgctcccga
     4261 ggctcgggcc gaggctgcgc tggctcgaga cgctgctgcg caacaccgtc aacgcgacga
     4321 tcgttcacgg cggcgacaag gtgaacgtca tcccggcgca gatcgacctc gagctcgacg
     4381 gtcgcctcct gccgggcttc acgccagacg acctcatcgg cgagctgcac ggaatcgtcg
     4441 gcgacgacat cgaggtcgag ctggtgcggc acgatcccgg cctcgcggag cccgacctcg
     4501 ctctcttcga gatgctcggc ggcgtgctgc gcgagctcga tcccgaaggc atcccgatgc
     4561 cgctgctgca aggcggtgtc acagacggcc gatttttcgc gcagctcggg atccagacct
     4621 acggcttcat gcccatgcgc ctgccggccg actttccggt cctgaagatc gtccacgccg
     4681 cggacgagcg catccctgtg gctgccctcg agttcgggac ggaagccgtg actcgggcac
     4741 tgcagcgttt ctgatgtccg acaaggacta ctcggggacg ccgctgggca agaagctggg
     4801 cgcgaggccg ggcgtggagg tgctggtgct cttcacgacg aatcgcgcag agctcgagcg
     4861 ccgctttgcg acgttgaaga agacgctggc gcccgcggac gggctgtgga tcgcctggcc
     4921 caagaaggcc tcgaagatcg agaccgacct cgacttcccg accgtgcagg agaccgggct
     4981 cgctgccggg ctggtcgaca acaagagcgc tgcgatcgac ggggagtggc aggccgtccg
     5041 cttcgtctac cgcaagaagg atcgcgctcg gtagcatcgc caggatcgtg agtgccttca
     5101 gtctcgagcg gctcgacaac gggctgcgga tcctgaccgc gccgatggac tcggctcaat
     5161 cggtgacctg cgcgatcatg ctcgcggccg gctcgcgcta cgagacgccc gagaccaacg
     5221 ggatcgcgca tttcgcggag cacatgttct tcaaggggac ggagcgcagg ccgaccgccc
     5281 gggacatctc catggagatc gacggcatcg gcggcgaatt caatgcattc accggcaagg
     5341 agtacaccgg ctactacgtc aagtgcgccg cagagtcccg cgacatcgcc ctcgacgtcc
     5401 tcgtcgacat gctgcgccac tcgaagttcg atccggagga gatcgagcgc gagaaaggcg
     5461 tgatcatcga ggagatgaac atgtactacg acacgccgcg cgacttcatc ggcggcgtgt
     5521 acgaagagct gctctacgac gatcagccgc tcggctggga gatcatcggc cgcaaagaga
     5581 cgattcgcaa cgcgacgcgt gagatgttcg gcggttacgt cgatcactgg taccagcctt
     5641 ccaggatggt ggtcggagtc ggcgggaaga tcggcgacga cctgaacgag agggtccagg
     5701 agctgctcgg cgacgtggcg aaggcggaga ccggacgtcc tgagccgatc gaggtggatc
     5761 cgcacgacca cccgcacgtc aaggtgcaca cgaaggcttc cgatcaggcg catatctgcc
     5821 tcggcgtgca cagctatccg ctcgagcatc ccgatcgcta cgtgctgcag gtgctcgcga
     5881 cggtactcgg cggcgggatg tcgtcacgac tcttcacgga ggtccgtgag cgacgcggcc
     5941 ttgcctacta cgtctacggg ctgaaccaca gttacacgga tgccggctcg ctcttcacgc
     6001 aagcgggcgt cgacatcaac aggatcgacg acgccgtcag cacggtcgcg gccgagctcc
     6061 gcaagatcgc cgaggaaccc gtgccggccg ccgagctcga gaaggccaaa agcttcgcga
     6121 aggggcgatt cgtcctccag ctcgagacct cgcagggatt gatcatgttc ggtctgcggc
     6181 gcgaggtgct ggagagcagg acgccggatc cggaagagat catcggcgag ctcgacaagg
     6241 tgactgccga ggacgtggcc cgcgtcgccg gcgacgtgat cgagaagcag ggattcaacc
     6301 tcgccgtgat cggcccgttc gacgacccgg accggttcga gaagctgctc gcctgacggt
     6361 gcgctatctc atgggcgcat gctgtcttgc gctcgtggcg gccggctgcg gcagcagcgg
     6421 gaagtcggcg gcgacgacga agccgacgac gaccgcgaac acgacgacga cgaccacggt
     6481 caccgtcggc ggaacgtacc gctactcgca gaaggtgatt cgcagcttca tgcgaagttg
     6541 cacgcagggg aacagcgcca aagaggatta ctgcgcctgt gtgatcgaca agctctcgaa
     6601 cagcgtgtcg actcgcgact tcgcccgcat agcacgcacc ggaagagcgg ttccgcgagt
     6661 cgcgagagcg atcaggcggg cggcagccgc ctgcgcggga actggttgat ctagagggtc
     6721 agtcctcgcc cgcgtcggtc gctgcgcgtt ctttgatctc ttcgacgacc gtcggatccg
     6781 ccagcgtggt cgtatcgccg agcgtgcgct gctccgcgac gtcgcgaagc aggcgccgca
     6841 tgatcttgcc gctgcgtgtc ttgggaagct ccggcgtgaa cacgatgttc gccggcttcg
     6901 cgatgggacc gatcttcttg gcgacgtggt tccgcagctc ttcaagcatc tcgatactgc
     6961 cttcctcacc gcctttgagc gtcacgtagg cgacgatcgc ctggccggtc cggtcgtcct
     7021 tccggccgca gacggccgcc tccgccacct tcttgtggtc gaccagcgcg ctctcgacct
     7081 cgatcgtcga gatgcgatgg cccgacacgt tcatcacgtc gtccacacga ccgagcagcc
     7141 agaagtcgcc atcctcgtcg atccgcgcgc cgtctccggc gaagtagatg tcgtcgtact
     7201 tcgaccagta cgtgtcgcgg aagcgctgat cgtccttgta gatgccccga agcatcgcgg
     7261 gccacggccg cttcagcacg aggtatcccc cgccgcccgg accgacctcg tcgccgctct
     7321 cgctgtacac gcccgcgtcg acgccgggaa acggcttcgt cgccgagccc ggtttcgtcg
     7381 tcgtcactcc cggcagcggc gtgatcagga tcatgccggt ctccgtctgc caccaggtgt
     7441 cgacgatcgg cgtccggtcg ccaccgatgt gctcccggta ccagatccac gcctccggat
     7501 tgatgggctc gccgacgctg ccgagtaggc gcagcgagga gagatcgtgc ttctgggcgt
     7561 gctccggtcc ccacttcatg tgcgagcgga tcgccgtcgg cgccgtgtag aggatgtcga
     7621 ccttgtagcg ctcgatgatc tgccaccagc gttcgggatt cggatagttc gggacgccct
     7681 cgtacatcac gccggtagtt cggttcgcga gtgggccgta gacgatgtag ctgtgcccgg
     7741 tcacccagcc cacgtcggcc gcgcaccaat agaccgagtc cggcttgaca tcgaagatgt
     7801 agtggtgcgt cgcggccacg ccgacgagat agccggccgt cgtgtgcacg atccccttgg
     7861 gctttgcggt ggtcccggac gtatagagga gatagagcag gtcttcggcg tccatcggct
     7921 cgggaggcgc agaggccgga tcgtcatcct ccccttcgac gagctcgtgc caccaggcgt
     7981 cccgagacgc gtcgaagggg acctgatcgc ccgttcgttt cagcacgacg acgttcttca
     8041 ccgtcggcgc ctgcgccacg gcttcgtcgg cgttctgctt gagcggcacc ttcttgccgg
     8101 cgcgccaacc ttcgtcctgc gtgatcagga gcgcgcactc catgtcgttc atacgatccg
     8161 acagggattc gctcgagaag cctccgaaga cgacggtgtg cggcgctccg aggcgcgcgc
     8221 acgcgagcat cgcgattggc agctccggaa ccatgcccat gtagatgccg accggagtcc
     8281 ccttcgcgac gccgagtttc ttgagcgcgt tcgcgaagcg cacgacctcg cgctgcagat
     8341 cggcaaacgt gatcgtgcgc cgctcgtctt ccggctcgcc ctcccagaag tacgcgacct
     8401 tttcgccgtt gccggcctcc acgtggcggt cgacgcagtt gaagcagaca ttgagcctgc
     8461 cgccgaggta ccacttcgca tacggcgcct cccactcgta gagcttggag aaaggctcga
     8521 accacgtgac gcggtcgcgg ccctcggtct cccagaactc ctcccagtcg cgctcgtaga
     8581 tctcgggctg agcgttggcc tgccgcgcga actcagggtc gggggcgtaa cgacgctcct
     8641 ccaggagcat cgtgtcgatg gtgttggtca aggcgccagg tcgtacgggt aatccgtgag
     8701 gacttcgtgg ccgtcgttcg tgacgaggac gaggtcctcg aggcggaggc cgccataccc
     8761 ggaccgatag agcccgggct cgatcgtgac gacgtcgccc ggcacgaggt cgtgcccgat
     8821 ccggctcatc gaaggcaact cgtgcacctc gagcccaacg ccgtgaccca ggccatggaa
     8881 gaagccgtcc tccagcacct cgcccggctc cttgttcagc tgcgtcttgt agccggcgct
     8941 ctggaagatg tcgcagacct cttcgtagag cttgcggccg ttcacaccgg gcttgactcc
     9001 ggcggtggag gtgtcgagcg cctccttgac gagcctgtgc cactccttca cctcgtccga
     9061 gggctccccg acgacatagg tgcgcgtcat gtccgcgtag cagccggtcg ctttgtccct
     9121 ggggaagaga tcgaagacga tcggctcgtc cgggccgatc tgaccggagc ccatgttgtg
     9181 gccgactgcg gtctgcgacc cgtgcgacac gatcatgtcg ctcccctcga cctcgtggtc
     9241 ggcgaagacg tcctcgatga tgcgcttcag ccgctcgcag gtgagcggct cgccgtctac
     9301 ttcgagaccg gcgccgttcg gctgcgcccg ccggagcaat tcgcgcgaag cgtcgagagc
     9361 ggcctcgcac gcccgttgcg cgttgcgaat cccgcgcagc tcggtctcgt tcttcgagcg
     9421 ccggcgattg tcgaagaggt cgcggtcgac gacgagctcg actccgttcg tgcggagatg
     9481 gtcagcgtgc ccgacgggaa agctctcggg gacggtggcg cgctcgatcc caagctcgcg
     9541 gcaggctcgg acggcgagct ccagcactac cttccagccc tgcagaccct gcgtgaagag
     9601 ctcgtcggag ccgaggtcct cgagcgggac gatatccagg tcgggcgcgt cctcccgcac
     9661 gcgtgggatc tcaagggagt ggaggaccac gatccgccgg ttgcccttct ccgcgtagag
     9721 gaaggggtcg gggatcggca ccggcacctc gtggcgcagc tcgggcgagc gaatcgtgtc
     9781 gccatagata agtacgtcgg gcacgggcgg atcctaaccc ggcgctacgc ccaacggaga
     9841 aaacgggtca ctgcatagac tccaaaccgt ggacgacgcg ccgcagcgtg cccagaagtt
     9901 gatggctcag tgggacgacg agctgcgcaa ggaactgccc gcaggcgccg acatcttcga
     9961 tgcgcacacg catctcggca ccgacatcga cggcatggtc gggatctacg aggacctcga
    10021 gcgcgggatg gacaagtacg gcatctcgcg ctgcttcatg ttctgcctgg acgagcccga
    10081 ccggcatccc gggttccggg cgggcaacga caggacgctc gagttcggcg ggcgctcgcg
    10141 cgggaggatg atccctttcg tccgcctcga tctcgcggag gacccgatcg gcgaagcgac
    10201 ccggatgctg gacctgggcg ccaagggcat caagctccat ccgcgggcgc agaagttcct
    10261 gctgaacgac gagcgcctct cgccgatctt cgagctcgcg gccgagcgca aggtcccgat
    10321 cctgatccac ggcggtcgcg gcctgccgcc gatcgcggac gacctggcac gcgtcgtcga
    10381 ccgttacccg gacgcgcagt tgatcgtcgc gcacgcggga atcgccgacc tcgcagcgct
    10441 cgccgaccgg ctcggcggga aagcgggcgt cttcttcgac acgtccgtct ggagcccgct
    10501 cgacctgctc agcctctacc ggctcgtcgg tccggaacag gtcgtgtacg catctgacta
    10561 tccctatgga caacagccgg cgtcactgtt gatctcattg cggaccgccc gcctcgcaca
    10621 gctgaccgac gaacaggtcc gcggagtgct cgcgggcaac gccaaccgca tggcagacgg
    10681 cggagcgccg gtcgagccct cggccccgaa gggcctcgag accttctcgc agccgatgtc
    10741 gctggcgcgg atccaccagt acctgtcgat ggcgactccg ctcctgtgga cgagacagca
    10801 ggatcgaatc ggcgtgctcg gcctggcgct gaacgcctgc gacgaccggg cgaacgggca
    10861 ccgcgaagag ctcgatcaga tcagggagct cctcaccgtc gcgcaggaca tgtggttgac
    10921 actgccggag gatggggacg aaagggaccg catggcacac acgcgcgcga ctttccgcct
    10981 catccaccta gccgacatcg tggcggtgac gacgcctgcc tgagctgcgc atcaacggcg
    11041 agacgcattc gttcgacgcg ccggtcgaac agtcgctcgc gcaagtcctg cgggaggacc
    11101 tcggcctcac cgggacgaag gtggcgtgtg gcgagggaca ctgcggtgcc tgcaccgtgc
    11161 aactcgacgg cgtgccgacc ctttcctgca tcacgctcgt gcacaccgtc ggcaaccgcg
    11221 aggtgacgac catcgaaggt cttcgcgacc atccgttgat tcacgccttc gtacggacgg
    11281 acgccgtcca atgcgggttc tgcacgccgg gtcaggtcgt ctccgcggcc gcactcgtcg
    11341 agggaaatcc gaacccgtct acggacgaga ttcgtcatgc catgtccggc aatctctgcc
    11401 gctgcggtac gtatccgaag atcgaggagg ccatcagaac ttggcaagac tgatccgcac
    11461 cgaaaaggaa gtcgaaggac gctacgagga agtctggatc gtcgtcgacg aggacgcgct
    11521 cgaccagtgg ccggcgggac cgctcacgac tgtggggcgg agagtgccgc gggtcgacgg
    11581 actggcgcgg gcccgcggtc aggcgcagtt cacggccgat ctccaactgc ctgggatgct
    11641 gcacacggcg gttctccgta gtccctacgc gcgggcgcgc gtgacctcgc tcgacctgga
    11701 accggcgcgc agcgcgcccg gagtgcgtgc tgcgatcggc ccggcggacg aggatcagct
    11761 caccgccgag ccgagctacg tcggccacgc ggtggcggcc gttgcggccg acacgttcag
    11821 ccaagcacag gcggccgtcg aacgcatcgt cgtcgactgg gaagtactcg aaccgttgct
    11881 cgacccggac gaggcggtgc gacaggagtc gttcgtctcg gacgtggcca ggtacgagcg
    11941 cggggacttc gagcgcgggc tcggagaggc cgacgtggtc gtcgaggccg agtatcgaac
    12001 gcaggtcgtg gtccacaact cgctcgagac acatcaggcc gtctgtcact gggaggacga
    12061 aggcatcacg atctacatct cgacccagta catctggggc atccgcgcgg ccatggccgc
    12121 gcacctcggt ctgccacccg acaaggtacg tgtcatctgc caggcgatgg gcggcggctt
    12181 cggagcgaag aacagccccg gcgactacac gccgatcgcg gccgagctcg cgaagaggac
    12241 ggggcggccg gtcaggtgcg cgctcactcg gcgtgaggag aacgtcgccg ccggcaaccg
    12301 caacgcaacc atccagaggc tgaccgcagg cgcgacgtcg gacggcacgc tcgttgccct
    12361 cggcggtgac ttcgtcaacg ccgtcggctt ctcgggctgg tcggccacga cggaagggcc
    12421 gatgaagatg ctctattcgt gcgacaacgt gcgcacgacg cagtatccgg cgaagctcaa
    12481 cctgccgccg atggccgcgt ttcgagctcc gggcttcgtc gagggcacgt tcgcgctcga
    12541 atgcctgctc gacgagctcg cagtgaaact cggcgtcgac ccgctcgagc tgcggcgccg
    12601 caactacgcc gactccgacc tcaccgacga ccgcccgttt tcgtcgaaga acctcgccga
    12661 gtgctaccga cgcgcagagc cgcactgggc gaagcgcgac gaggtacgcg cgcgcagcca
    12721 gggcccgtgg aagcacggca taggtctcgc gtcgcagatc tggtacggag gcggcggtcc
    12781 gccctcgtac gcgtggatcc gcgtcggttc ggacggccgt gcacaggtcg tcacggcgat
    12841 gcaggacatc ggcaccggca cgcgcacggc catggcccag atcgcagcgg aggagctcgg
    12901 cgtgccgctc gggcacgtgc aggtctccct tggcgactcg gctcgcggac cttttgcgtc
    12961 gatttccgcc ggctcgtcga ccgtgccgtc gatgggaccg gctgtgcgct cggccgcagc
    13021 ggacgccaag cggcagatca tcgagatcgc ggcgcagcgg ttccacctcg aagagcgcgt
    13081 gctcgatctg cgtgagggcc ggatcgtgtc cgccgacggc ggttcgtggc cgctcaccga
    13141 agtcaccggc ctgctcgaga gcggtcagat tctcggcaag ggagcgcgcg ggccgaaccc
    13201 ggccggcatg caagtgctga cgttcggcgt ccaggtcgcc gaggtcgccg tcgacgtcga
    13261 gacaggagag gtgcgcgtcg acaagatcgt cgcgatccac gacgtcggcc gcgtcatcaa
    13321 cgcgctcggg gcggagagcc aggtcgaggg cgggatcatc cagggcatcg ggcacacgct
    13381 gtcggaagag cgcatcgtcg atccagcgac gggctcggtt ctcacgcgca cgctcgacgc
    13441 gtatcggatg ccgacgatcg cggacgtgcc ggaaatcgtc accgagctcg tcgacatacc
    13501 ggacgaacat ctgacgaacc tcggctccaa aggcctcggc gagccgccga tcattcccgt
    13561 cgccgccgcg atcgcgaacg ccatccgcga tgcgaccgga gccgagattc gctcgctgcc
    13621 gatcactcgc gaagagatgt tccgcgcctt gcgcgaagcc gacgcgcgtg agaaggagcg
    13681 gcgtggagct gctgcggccg tctagcgccg aggaagcgac cggggcgctc ggcaacggct
    13741 cgcgcgccct cgccggcggc accgagctcg ttccgctcat gcgcgacggc ctgctcaagg
    13801 ccgagacgct cgtggacgta cgcggcgttg tgccgcgggg gatcgccggg aaccgcatcg
    13861 gcgcgggaac gacattggca gagctcgagc tcgatgcgca gatcccgcaa gcgctgcggg
    13921 aggcgtgcgc acaggcggcc tccccgcagc tgcgctcgat ggggacgatt ggtggcaatc
    13981 tcctgcaggc gacgcgctgc tggtactggc ggcttggctt cgactgctgg ctgcacggcg
    14041 gcgagaaatg cttcgcgaag gacggggcgc agcgcgagca cgcgatcttc ggcaacgagc
    14101 agtgcgcgtc cgcccatccg tcagacgtcg ccgcggcact gctcgcactc gatgcgacac
    14161 tgcggacgac gcgacgtgaa tttgcagtcg cggagctcta ccgtttgccg accgaagaga
    14221 accgcaacgt cacgacgctc gcacccgacg agttggtcct cgagatcgag ctcacgacgc
    14281 ccgatgcgtc gacgtacctc aaggcgatgg accgccggaa gtgggcattc ccgatcgtcg
    14341 gcgtcgccgc ggcacgtttc ggtagcgaaa ctcgattcgc tctcgccggg gtggctccga
    14401 tcccgtggct gctcgactcg gccgcagctc tcgacgacgc cacccctctt cccggcacgg
    14461 cctacaaaat cgagatcgcg cgcgccctcg tccgacgcgc aactcacgcc gtaaagtccg
    14521 cctcgcgctg acgggcgagc catgacgact ttccggcgag agatctccga gcgcggttgg
    14581 gggccacggc gggcaaagcc caccctggcg gcggtctttg gtctcgcctt gttcgccctg
    14641 gctggatgcg gtggagggac gaagcagcag gctgcgacga cctgtccgac gacgggcaag
    14701 caggtgacgt tcccgctgct ggacaagtcg aaggcgtcta cggtcggcgt cctcaccaac
    14761 ttcggccgct tcgacattcg cctggacgtg cgggactcgc cgtgcacgac ctcgtcgttc
    14821 gccgcgctgg tgcggaaggg ctacttcgac ggcacgcgct tccaccggat cgtgaccggc
    14881 ttcgtcatcc agggtggcga cccgaccgcg acgggtcagg gtggtccggg ctacacggtc
    14941 caagacgttc cgcctgcgaa cgcgacctac accagaggcg tcgtcgcgat ggcgaagacg
    15001 aatgccgaac cgcccggtac ctcagggagc cagttcttcg tcgtgaccgg cgcagatgta
    15061 gggctgccgc ctcagtacgc catcctgggc accgtcacga agggcctgaa ggtcatcgac
    15121 cgaatcggca agctcggtaa cccggcaaac gaacgcccca ctcgcaaggt cgtcgtgcag
    15181 cgaatgaccg tcacgccgta acgtaagcga gatgatcgcg gccgtcgtgc tcgccgcagg
    15241 ggcgtcgacc cgcttcggct ccccgaagca agccctgctg ctcgagccgg tcctcgaacg
    15301 cgtgcgatcg agcgccacga tcgggaacac catcgtcgtg ctcggcgcac acgacgtcga
    15361 gaccaacgcg cgtgttgtgc gctgtcccga gtggcaccgc ggcccggggg cgtcgcttcg
    15421 ctgtggtctc gacgcactcg gcgaggagat cgaggccgcc gtggtcgtcc tggccgacgg
    15481 accgtccctc gatccgcgtg ccatcgaccg ggtggtcgca gcgtggcgcg agagcggtgc
    15541 ggagcgagtt gccgccacct acggaggcat ccgccttcac cccctcctgc tggcacgcga
    15601 agcctggagg gaggtgccgg accaagggct gcgcggcctg gcggccgagc tcgtgccctg
    15661 cgacgatctc gatcctccag gagacgtcga ttttgccgat gacttgagct gaaggaggat
    15721 ggccgccccc gaaaccagaa tgtggtcgag catggagctc gtccaccccc tcgagccgac
    15781 cggcgaggaa tcgccgctgg cccgtgcccg tctgcaccgg cagctgactg tcgaagagac
    15841 cgcccggcgc gccggcatca cggccgaaga ggttcgctgg ctcgaggagg gccgggtcta
    15901 tcggttcccg aaacccgacg acgccttgat cgcaaccgcc ctgtacgcga cggcgctggg
    15961 cctcgacaac cgtgaggccc gcgagctcgc gggtttgccc gttccacctc agccgatcaa
    16021 gcggaacccg ctgccacgat tgctggtcgt gggcggagtc gtgatcgccc tcgtagtgtt
    16081 cgcgaccctg ctgctgatcc cacgttcagg cggcaagccg aagcagggca gcccgagcgc
    16141 aattctcccg ccgccctggc ggatccccgt caatgtcctg aacggcagcg gcgacatcaa
    16201 ctacacgcgg caagtggcaa gccgaatcgg cgcgttcggc tacaccatca agaaggtcgg
    16261 ccgcgccgac aatttcacgt atccgcaaac ggcggtgtac ttcccgccga aatgcgaggg
    16321 cgtggctttc cggctggcga agcaactggg tgtgccgacg aaaccgcttc cgggcggaac
    16381 cggcgtatgc cagctctacg tcatcgtcgg accggcgcgc ggtcctggcg aatagctcct
    16441 actaaagagg agtacgcagg cctgcgagct tggtcttgag ctcgactggc atgggttcct
    16501 gctgcactgc ctggcgcacg aactggcgca gcttctcctc gaagcgataa cgcttgcggc
    16561 agtaggtgca cccgtcgaga tgctgctccg cttccgcacg ctccgcgtcg gtgatcgtgc
    16621 ggtccatgta cggctgcatc atctcctcgc agtgcgcgca gggatcgtat gcagtcatgc
    16681 ttcctcgcct cccaccgcag actcggccat ggcttgcttc aggatacgcc gtccgcggtg
    16741 caggcgcgac atcacagtgc cgatcggaat gtcgaggatc tgcgcggcgt ccgcataggt
    16801 gaagtcaccg atgtccacca ggactacgac gtcgcgaaaa tcatgcggca cttccgagag
    16861 cgcgcccacg acgtcgttct gcgacagccg ctcgacgacg cggtcttcgt cgctcgcccc
    16921 gtcgcccgaa gcctcgagct tgttgtagag gaagtagtcg ccttcctcga gcggctgcgt
    16981 gtccggcgtg cgttgctgct tgcggccgcg gtcgatgttc aggttcgtga ggatccgcag
    17041 cagccaggcc cgcaggttcg tgccgggtgt gaacgaccgc cagctgcgaa acgcgcgcgc
    17101 atacgtctcc tgcatcagat cctcggcctc ctcgcgggag ccgacgagat ggcgggccac
    17161 tcgatacacc tgatccgaca aagcaagcgc gtcctcctcg aagcgcacgc gatcgcgcgc
    17221 ttcagtcgcc agttgccttg cgtcgggctc ccgtgtggtc tcgctcacct cgggccaccc
    17281 taccaaggcc ttcttggcgt aaacgtacgg ctgtccacac acattcctga gcggacctaa
    17341 ggcacttcgc ggaagccgcc gcgggccatt gcctcgagca gtgaatcgcg cttcgtgcgc
    17401 tcgtcatccc aggcaagcgt gacctggccc atcaggttgg cgttggcttc ctcgaggcct
    17461 tcgtgccctt cgagcacctt cgcgagccgc atgacgcagc gctcgcaacg gatcccgctc
    17521 atctggatcg tctcggtgac gacggccatc ctcttacgct accgaccgct cgacgagatc
    17581 tgcgcagaca gcagacaggc cttcggcacg gccgatctcg agttggcggt cggcctcgca
    17641 cgtctccggg tcgagtccgt gcaggcgaac aggcagctcg cgcgccagcg ccgcgacggt
    17701 gagcgcttcg tcgcggtccg gatgaacgag cttcccgtgc ggcccgaagc gcgaggcggc
    17761 ccagcggttc tgttcgtaga cgccgcgctc ggcgggctcg aacggtcgcg ggggcgcctc
    17821 gagcgcccag gcgcagagcg cgtgcaccag cccgaccagg gcagccgtgt gctcgaccga
    17881 ggtcgcctgg tccggcgccc gaacctcgag cgttccaaag cggggatgtg gccggacgtc
    17941 ccaccagaag ctcgtgtagt cgttcgccaa tccagtcgcc gccatccgct cgatgaaacg
    18001 ctcccactcc gcgtaatttc ggaaggcggg cggtgcgccg tgtcgcggca ggaagccgag
    18061 cacctcggcg cgaatcgaca gcatcccggt gtcgcgcccc tcgaagtacg gggagttcgc
    18121 agaaagtgcg agcacgagcg gcagccaggg caggatcgtc tccatgacgc ggaaacacgt
    18181 ttcggcatcg ggcatgccga tgtggatgtg caagccgctg acgccttgac gccgcgccga
    18241 tgggcccgcg tactcgacga aatcgcggta ccgctcgacc ggcgcgatct tctgctccga
    18301 cgggatgctc gtcggatacg agccccccgc ggcgacgacg tacccgtttt gcgcggcgat
    18361 gtcgcgcgcc gcccggcgta actcgaccag acgctcctgc gcctgctcgg cagtgtcgct
    18421 gacgtcggtc gtcagctcga cgaccgacgc gtgcagctcg gtcttcagag tgcccggaag
    18481 gttgcgtccc tcggcgccgg ccacgagcgt ctcgacggcg ggcacgagct gcagcgtctc
    18541 cgcgtcgacg atccagagct cctcctcgac tccgactgtc aaggaaggcc cgaactgctg
    18601 ctcgatcacc tcgccctgac gccgtggacc gggccgccgg ctgcttccca cgcgtagtac
    18661 gacgcgctcg ggccgtacga cggctcgtac gcctggatgc gtggattgcg cccgacctcc
    18721 ttggcgcgtt gtgcgtgatg cagcaaattc gggtcgtcgc gccgttccca gacgaggatc
    18781 agatgcgctg cggtcatgaa gccggccacg aaggtacagc cggccgccgg acaggggaag
    18841 ggattcgtgc gcacgcagaa ccacccgcta cggtcgccgg cttcgtcgtc cctccagagc
    18901 tcttcctccc agacgagctc cgcgtccgcc aggacgccgt gtcccggctc catccgcccc
    18961 tcctccaccg acaaaaggct acccgtcagg gacggccgtc ttcgggcact ctaggaccgg
    19021 ggtccgggca gcctggccag cagccgcagt ctggctaggc gccgggcgcg cctcgttgtg
    19081 cgcccacccg ggccccttta gtgcgccctc cagtcccgga acgcatgtcg cagcgcctcc
    19141 tcggcgtcct gcgacacgtc gtccacccgc agcgtgccgg tgagctcgat cgtgcgtctc
    19201 atctgctcga cgtacgtcgt gcagccgtcg caggccgaaa ggtgcgcgtc gaagcgcttg
    19261 cgctcgccgg aggacagatc gccctcgagg tacgccgtca cgagctcgac gagctccacg
    19321 cagttcagat gggccagacc gatgctcatt gttcctccga caggtactgc tcgagcgcgc
    19381 gcctcacctt ggaacggccg cggtgaagca agacacgttg attggtctcc gagagctcga
    19441 gagcgttacg gacctcgtcg gcggtccatc cctcgacatc gcgcaaggtg atgaccagct
    19501 gctggctcgg cgggagccgt tcgatggctc gttcgattac cgacaaggtc tccttcgcca
    19561 ggaggcggtc ttcgggccac gcacgcggca ggactgccca gtgcccggtg ccggtgaagc
    19621 gcgaacgctc gacagccggc tcgaagcccc cctcctccgt gtcgagagcc gagaatggaa
    19681 ctgaccgacg ctctcgctcg cctcgggtct tcgccgtgtt cgtgaggatg cggaagatcc
    19741 acgtcttcag cgacgagcgc ccctcgaagc gctcgatccc cttcagaacg gccagccagg
    19801 tctcctggac gacgtcttcc gccacggccg cagtaggcac gaacattcgc gcaacgcgca
    19861 ggagcgaagg gttcaggtcg cggatcaatt ccatgaaggc gtcctcgtcg cccgtccgca
    19921 gccgctcgac gagcctctca tcctgcgggg agaggaactg ccccatcgat tgactgtacc
    19981 gccgcctcgc gcggatctaa atcggggcga gcgctagcgt gcgcaggccg cgcacgtcga
    20041 cccagatgcg ttcgccgtcc gtcacgacct tcatcacgac cgcctcgcag tgcgggcagc
    20101 gcagaacggt gccggcggcc atgtagacgt ggatcgctcc gaccgcctcg gccgagccgc
    20161 agctgtcgca cgtgccgatg gccgtcgtca cctcgcccgc gaacacctcg ctgagaagcc
    20221 cagccgccgc attcccgtcg agcctcagtg cttccatctc atcctcctgt cgggccgaaa
    20281 cgctcggtct tgatgcgcaa cggctcgtgt ccaagcccga cgagcgcttc ggcgaccgcc
    20341 tcgacgaacg gggtcggccc gcacacgaac gtcaacgcaa gatcggcggg agcaacctcc
    20401 gacagcagct gccggtcgac tcggcggctg tagccggtcc agtcgggcgg ctggtcgcgc
    20461 gtgagcgtga acacgatctc caggccgtct tctcgtttcc ggtaccgctc gagctcatcg
    20521 cgatagatga cgtcctccca cgatctcgcc gagtagagca gacgtgtcgg caccgcatcg
    20581 tggttcgtcg cgcgctgccg caccatggcc atgaggggga cgatgccgga gccgccgccg
    20641 acgagaagca ccggcccgcc cggatccgga tcccagacga agtagccgcc gaccggcccg
    20701 cgcaactcga accggtctcc cgcgtgagcg tcctgggtca gatacggcga gacctccccg
    20761 tcgtcgatcc gctcgacggt caattcgacc cgaggaccgc cggcgggaga cgcgatcgaa
    20821 tagctcctct gggcctgata tccgtcgtca gcggtcagac ggacatcgac gtgctgaccc
    20881 gcccggtgac cggtccagtc ctggacgtcg agggcgaggg tcttgacccg atctgtctcg
    20941 ggccacgcgt ccgcaacctc ggcgacgtgc cagttcagtc gccccagtac cgctgttcct
    21001 tccaagggtc tccgtagatg tggtagccgt tcgactccca gaagccgggc tcgtcggtct
    21061 cggtgaggcg aagcccccgc acccacttgg cgctcttcca gaagtacaga tggggcacga
    21121 gcagccgcgc cggaccgccg tgctccggct cgagcggctc accgtcgtaa gcaaaggcga
    21181 cccacgcctt gccgtcggtg acatcggcca ggggcaggtt ggtcgtgtag ccgccgtcgc
    21241 agaaagcggt cacgtactcg gccgaggttt cgaccccttc gagcagcgca tccaccggga
    21301 cgccctccca gaccgtgtcg agcttcgtcc atttcgtcac gcagctgatg tcgaccgtga
    21361 tcgtctcgct cggcaacgcg cgaaactcgt cccaggtcca gcggcgctgc ccgtcaacct
    21421 cgccgccgat cgtgaagtcc cactggtcga gcggcgtgtg cggtgtcggg ccggccgaga
    21481 gcaccgggaa gtcgtccgtg acgtactggc cgggcggaac gcggtcacgc ggcgcgtcgt
    21541 cacgccttcg acccttgaac cctcgcgaga tgatcggcat ggcttctccc ttccggttcg
    21601 gtcagctgca cgcaggcagg agcgccgaca agctccatca tgctcttgta acagaccgag
    21661 aagcccctac gactgttacg ccagtgaaaa gaggaggtcc gatgaacgcc gagcgctacg
    21721 acctgatcgt cctgggctcc ggctcagcgg cacgagacgg cgcaggaaag gcagcgcgag
    21781 aacacggcgc ccgcgtggcg ttgatcgagc accggctctg gggcggatcc tgtcccaacg
    21841 tggcctgccg ccccacgaag gcctatgtcg tcgctgccga actgatgcac gacgtgcgac
    21901 accacgccgc ggagcgcggc atcgatcttc ccgagccggc ggtccggctc gcgcagacgc
    21961 ggaagtggaa ggacgctctt cgtcgcgacc agcggagctg gctcgagacc ttgaaggacg
    22021 ctgggtacgg cgtggtgccc ggaactgcgt cgttcgtgga cgcacggacg gtgcgggtcg
    22081 gcgaccagca gttgagcggc gagaggatcc tgatcgccac ggggagccgg acggcggtgc
    22141 ccccgattcc aggcatcgag gacatcgtgt ggatcgacca cgtcagcgca ctcgagctcg
    22201 acgaggtgcc cgaatcgttg ctcatcgtcg gtgcagggcc cgtcgggctc gagttcgcgc
    22261 agatcttcgc ccgcttcggc tcgcgcgtgg cgatcgtgaa cagcggaccg cagatcgcgg
    22321 cgcgcgcaga cacggaggcg gcgggcgagc tgcaggcggc gctggaggaa gaggggatcg
    22381 aagtcgtgtt gagcagcggc gtcgattccc ttgcacgcag cgggaccggc gtcgccgctg
    22441 ccgtcggcgg ccgcacgatc cacgtgtcgc acgtcctgct tgcctcgggc cggctgccga
    22501 acgtcgagga gctcgacccc gcccggatcg ggctcgagat cggtcgcggc ggcatcgccg
    22561 tcgacgaccg tcagcgcacg aacgtcggcg gggtctgggc cgcgggcgac gtcgccgccg
    22621 gcccgatgct cactccgatc gcgcagtatc aggcccgcat cgcggtcgag gacatgttcg
    22681 gcgacggctc gcggcgcgcc gactactcgg cactcccgac cgcgatcttc acggatcccg
    22741 aattgggcag cgtcggtctc accgaggcgg aggccgggga gcacggtcat gacgtcggcg
    22801 tggtcaaaca tccgttgccg tccgtcacgc gcgcgcagta cacgggggcg aagcacggtc
    22861 tctacaagat cgtcttcgat cgcgaaacgc gccgtgtgct cggcgtccac gtcgtctcgc
    22921 gcgcggcgag cgacatcgtc ggcagcatca cgcctgccct gaagctcggc gtcaccgtgg
    22981 acgacctcgc gttcatgcac cacgtgtatc cgagctacag cgaaggcttg aaagccgcag
    23041 ccgagaaggc cctagtttcg acgagcgaag cgaagtccta accattaacc ccgcggaaag
    23101 tgcgaagcac tttccgcgga gaacggagaa agagcaagag gccggggcag gcgttccggc
    23161 ctctcgctct gtgcttccgc gcgccgtgct ggcctacggc tccggcggaa gccggccccc
    23221 aggggtacgg ggccagtcgg ggcggcgttg agtcatggtt gccgcccaag cttgacgttg
    23281 acgtggatcc ggctcgttcc gcggacgacc tcgagatcga tgttgtcgcc cggcttcttc
    23341 gtcgcgatga gatcgacgag gcgctcgatc gacggaatcg gctggccgtc cgccttgacg
    23401 atcacgtcgc cgccggccgg ccagctctcc ccctccacgg tgacctgatt cgtcgcgggc
    23461 ttcagccctg ccttcgccgc gcccgtgcgg ggccgcaccg aggcgacgag cacgccgctc
    23521 gtgaccggaa ggtggaacaa gcgcccgatg ttcggttgaa gcgtcttccc ttcgatgcca
    23581 aggaacgcat gctcggcctt gccgtgcttg atcagctccg cgacgacgtc cttgacggtg
    23641 ttgatcggga tcgcaaagcc gatgccgacg ttgccctggc tctgaccgcc ggtctcgatc
    23701 tgcgcgttga cgccgatcac ctcgccctgg gcgttcagca gcgggccgcc ggagttgccg
    23761 tggttgagcg ccgcgtcggt ctgaatgacg tgcgagatcg agagctggtt gggcgcgctg
    23821 atccggcgct gcacagcgct cacgatcccg gcggtgacag agcgatcgag cccgaagggg
    23881 ttgccgatcg cgattacctg gtcacccacg cgcaccgcgt cggaattgcc gagcggcaat
    23941 gacttgaggg cacgcgactt gacgtgcacc tgcagcaccg cgctgtcggt cgacgggtcg
    24001 acgcccacta ccttggcctt gaagcgctcg ttgtccgaga agctcacctg gatcgtgttc
    24061 gcaccccgga cgacgtggta gttcgtgacg atgtgaccgg ccttgtcgat cacgaagcct
    24121 gagccgagtg cgcgctgagt ctgacttgct ccgaacgggt tcccgaagaa gtcggagggc
    24181 ggctgcgcgc gcgacgtcgt ctcgatgtgc accaccgcag gcgccgacgc acgatagatc
    24241 tcgttgatcg acatccgctt gccctgcgcg aaggcggcgg gcgccgagga gctcggcaca
    24301 acctcctggc ggatcacggt cgtgtgctca ccaagcttcc ccagcgcggc cgcgccgcca
    24361 agcgcaacgc cgccgcccag gagggcaacc gcgaagagcc cggcggcgcg gctccggagg
    24421 tggaggaagt gatgcacgct ctcgtttcta ccgagggcgg gcttaacgca cggttgcgct
    24481 ccgttaagtt cccattaagg cctgtagagg agagcgagct ggttggctga gcggcccggc
    24541 ggaatggaga ggcggatggg cttcgcaaag agcgagcttt taccgcgcct ttgagcggtc
    24601 tttacagggc actaaaaccc gcttcctagg gtaaggagct tcaatcgccc acgacaaggg
    24661 ggaattcgac atgcgtttgc tgaagagatt cacgccgtcg cccgcgatgg tgatcgcttg
    24721 cctggcattg ctgctcgtgc tcggcggcac cggttacgcg gcgagcaaag cgctcccgcg
    24781 caacagcgtc acatccgtgc aggtgaagga ccactcgctt ctggcacgcg acttcaaggc
    24841 cggccagatc ccgcgtggcg cagccggccc tgccggacct gccggccctg cgggccctgc
    24901 aggtcccgcc ggacccgccg ggacgaccgg ttcggcgtcg gtcaagtggg cgctcgtacg
    24961 ccctgacggc ggcatcgcgg cgcagtccgg cggcattacg ctggccgcac acccgagcac
    25021 ggggacttac atcctcaaca tcggtagcgc cattaccggc aagccgatcc tcgcgagcgg
    25081 tggctacgcc ggagatgcag gcgaccagcg cggagagacc accgccggtc cctgcggcgg
    25141 cggcagcgaa ggcaggacct gcccgagcgg cttcgacacg acgagccatc tcttcgccca
    25201 gacacggaac aacgacggcg ttgtgactga tcacgcgttc tacgtcgtcg tcttcggcta
    25261 accgaaacgc atctgagggc gcggccaccg gctgcgccct cagaatgcag gaatgagagt
    25321 ttcggccaaa gcggactacg cgctccgagc agtgatcgag ctcgccgtca tcggcggtga
    25381 cgggcccgta aaaggagagc gcatcgcaca ggcccaggag atcccgctga agttcctcga
    25441 gaacatcctc ggcgacctgc gacacgcggg cctcgtgcga agccagcgtg gtgtcgacgg
    25501 cggctactgg ctcgcgcggc cggccgacga gatcaccgtg gccgaagtga ttcgcggcgt
    25561 cgagggcccc atcgccaacg tgcggggagt cggccccgag caggttgagt acgccggatc
    25621 agcagagcgt ttgcgcgagg tgtggatcgc ggtccgcgcg agccttcgcg ccgtgttgga
    25681 gcaggtgacg atcgcggatc tggccagcgg cgagctgccg ctctcggtgg aagagctggc
    25741 gtcgaatccc gacgcctggc aaccgcacta gggaggcggg ctaatcggtg aagaccgtct
    25801 gccgcgtcgg gcggacgtac acgatctgac catgctgaag ctccagggcc tccacttgct
    25861 cgcgggtgag ttgcgccgac atcgtctcgc cgtcgtcgcg aacgagctcg acgcgaacct
    25921 cgaagcccag atgcactaga cgctgcacca tcgcctcctg cgtcgagccg ttgggctcga
    25981 gcagcagctc gacgtcgtgc gggcggatga acgtgtcgcc gagacgattg accgggccga
    26041 cgaacgacat cacgaactcg ttggcagggt tgtcgtagag ctcgcgtggg ggagccgtct
    26101 gctcaatccg gccggcgttc atcaccacga cgcggtcggc gacgtccatc gcctcctcct
    26161 ggtcgtgcgt gacgatcacc gtcgtggtgt gagtctcgtc gtggagacgc cgcagccaga
    26221 gacgcagctc cttgcgcacg cgcgcatcga gcgcaccgaa cggctcgtcg agcaacagga
    26281 ccttcgggtc gaccgcgagg gcgcgtgcaa gccccatgcg ctggcgctgg ccgccggaga
    26341 gctgggccgg atagcgtttc gcgaggccgc tgagttggac gaggtcgagc aactccgaca
    26401 cgcgctcctt gatctgggtg cgcgggcgtc gccggatcgt caaaccgaag gcgatgttgt
    26461 cccacaccgt catgtgcttg aaggcggcgt agtgctggaa gacgaagccg acgccgcgag
    26521 cctgcggctg ccgccgggtg acgtcctcgc cgtcgaggaa gacctcgccg gcgtccggct
    26581 gctccagccc tgcgatcacc cggagcaggg tcgacttccc acttccgctc gggccgagga
    26641 gcgccgtcag ggagccggtc ggcacgtcga cggagacgcc gtcgagggcg gtgaaggcgc
    26701 cgaagcgctt gacgattccg cgcgcctcga tcattggatt ccctcttttg gcttcagccg
    26761 ggtcatgagt aggagagtcg ccactgccag gagtgcgagc agcaccgcag cagcataggc
    26821 accggtggtg tcgaacgcct ggtaccgatc ctggacgtag agggtgagcg actccgtctg
    26881 cccgaccaga cgaccggaga cgacgctcac cgcgccgaac tcgccgaggg cgcgggccgt
    26941 ggtcagcacg accccgtagg cggtcgcata gcggattgcc ggccacgtca ctcggcggaa
//
LOCUS       DASVEU010000119         9691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_855386,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000119 DASVEU010000000
VERSION     DASVEU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855386"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1482
                     /gene="gatB"
                     /locus_tag="VF225_04920"
     CDS             64..1482
                     /gene="gatB"
                     /locus_tag="VF225_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7144631.1"
                     /translation="MPEWEPVIGLEIHVQLKTDTKMFCGCESGFGGEPNTRTCPVCLA
                     FPGALPVPNATAVEWTIKLGHALNCRIAERAVFHRKNYFYPDNPKGYQISQYDEPLCI
                     AGSFVVPGPEGDTEVGIVRAHLEEDAAKTIHVGGETGRIVGSAHSNVDFNRGGTPLVE
                     IVTQPDLRSADDAKRFLQLLRQTIVELGISDAEMEKGTLRADANVSVREVGETGYRTR
                     TELKNMNSFNHIARGIEAELRRQIETWESGGEVVQETLDYEVRSDTVTPRRRKEEADD
                     YRYFPEPDLVPIEPEAEVVHRLRAEVPELPGVRIRRFEQQYDLSYYDAEVLNGSAALA
                     ALYEQVALDGIDAKAASNVLMNDFVATGVDPAAVNGAELAKLIEARATIPRETFTRAL
                     TESADPEFRAETYLGDALIADRAELEPLVERILATNPGQVAAYKGGKAGLLGFFVGQV
                     MKETEGKADPKVVNDLLRQKLA"
     gene            1554..2165
                     /locus_tag="VF225_04925"
     CDS             1554..2165
                     /locus_tag="VF225_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144632.1"
                     /translation="MDTWVWIVIGLVVGIVILGILISALRTRRSRSLRDRFGREYDRT
                     VDKTGGRREAEQELRKREQRHDELELRPLSADARERYLRQWQVTQGRFVDDPTGAVSE
                     ADNLVQQVMRDRGYPVDDFEQRAADISVEHPEVVEKYRTANGIARASERGEASTEDLR
                     HSVRHYRALFVELLEVDDEAEDASTRDRQVEEVRAHDNVERLR"
     gene            complement(2177..2737)
                     /locus_tag="VF225_04930"
     CDS             complement(2177..2737)
                     /locus_tag="VF225_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144633.1"
                     /translation="MHSKLTLAFACACALGVALACAATIRADSTPEPPPESTTAEPQW
                     TQRDIQRQIDRYRHETWRWQRTMGRKLTRKLDDPPADVQTKIAVWQRVALKTRERAQN
                     PPHRRQWLCIHRFEGAWTDPNPPYYGGLQMDLSFQRAYGGGLLSRKGTANHWTPLEQM
                     WAAERAHRSGRGFYAWPNTARYCGLI"
     gene            3022..4176
                     /locus_tag="VF225_04935"
     CDS             3022..4176
                     /locus_tag="VF225_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX7144634.1"
                     /translation="MAEKRYLLTPGPTPVPPEVLAELAKPIIHHRERDFREIYERCLV
                     RLQEVYRTRNDVLMFTTSGTGAFESAVANLTSPGDRQLVLSAGNFGERWAGMARAFGA
                     DLVHARLEWGETPEPEDLRSALADAGDVKVVYLTHSETSTGVVCDVQALAAVAKDAGA
                     LVVVDAVSSLGAVPLESDLWGVDVVVSGSQKALMCPPGLAFASVSAAALAAATGAGSP
                     RYVMDWERTRKGQAKLDAPFTPAVSLVRALDVALGLLLDEGLEAAFDRHARLGRACRE
                     GAKAMGLELFSPDEERSAVVTAIRAPDGVDSTEVVKALRDRFGITIANGQADLKGKIF
                     RIGHIGWFDIFDITTALAAVELVLADAGAEIERGVAVTRALEAWAEHTPV"
     gene            4173..5744
                     /gene="serA"
                     /locus_tag="VF225_04940"
     CDS             4173..5744
                     /gene="serA"
                     /locus_tag="VF225_04940"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7144635.1"
                     /translation="MSKVLVREEIAEAGVRLLRDRGFEVDVDGDSDLAETIGRYDAII
                     VRSATRVTADLIARADNLKVIGRAGVGIDNVDVEAATRRGIVVANAPESTVISAAEHT
                     IGLLVALTRNIPQAHAALKQGRWERKTYGGVELADKTLGVLGFGRIGQQVARRAAGLG
                     MRVVAYDPFVSRDRFRELGVERVENQDDVYAAADFLTLHLPLTDETRESLNAHAFGKM
                     RDGVRIVNAARGALVDEADLLDALKSGKVGGAALDVFSTEPYSGPLLELDNVVVTPHL
                     AASTEEAQDRAGVIIAEQVAAALQGGLVSNAVNIPVIGAEDLEVLGAYIPLAAKLGRL
                     VMELAEGRVEELRLTYFGALAQYDTRLLTVAALNGAFQGRSDQPVNYVNAPMIAAERG
                     VDVREERSRSARDYTNLVRVEAVFGGEPLRVAGTTMGNDNRLWLVSALGFELDMELAP
                     LLVVFRYDDVPGVIGKVGTLFGAAGVNIANMTVSRTRQGGQALMALSIDSPAPPELVQ
                     QIHSEFDDARFISLE"
     gene            complement(5746..6087)
                     /locus_tag="VF225_04945"
     CDS             complement(5746..6087)
                     /locus_tag="VF225_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144636.1"
                     /translation="MAEREERIAHTEALFRDVNERIAESADRFDAVDAEFVCECADPE
                     CAERVPATLDQYEEVRSDGTHFLLHAGHEVPEVERVVKSPHKRFVIVEKFNSVVARTV
                     RRLDPRAGDAL"
     gene            6143..6553
                     /locus_tag="VF225_04950"
     CDS             6143..6553
                     /locus_tag="VF225_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144637.1"
                     /translation="MRHVWPEAVERIAAFLRVSGVEGRLEELLPGAGPPPGQKLSAKG
                     FDGNGAAMVAIVPVGRSVDERKLAAAAGCDEVQPAAATVFPFAEDRVFMDQSILSFDA
                     VWLEAGSSRHILGLTPVQLARVTNAETADLVREG"
     gene            6626..7561
                     /locus_tag="VF225_04955"
     CDS             6626..7561
                     /locus_tag="VF225_04955"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7144638.1"
                     /translation="MQELEKIWMNGELVDWADAKIHVGSHGLHYGSGVFEGIRAYETP
                     KGSAVFRLTEHLERLQSSAQVLNMELPYTVAELRSATMELIGANGVAECYIRPIAFFG
                     FGELGVASTGNPVDVVIMSWPWGTYLGPEALKNGIRAKISSWTRVGPNVIPHVAKATG
                     IYLNSMLAVMEANRAGYDEAILLTDDGYIADGSGENVFIVKDGTIYTPDLSASILRGI
                     TRDTIIQIAQDLGHSVVEKQLIRTDLYLADEVFMTGTAAEVTPLREVDDQTIGPPGPV
                     TLEIQKAYLDTVRGGSERWSHWLEYASTEPARAEA"
     gene            7558..8694
                     /locus_tag="VF225_04960"
     CDS             7558..8694
                     /locus_tag="VF225_04960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7144639.1"
                     /translation="MTEAVREIPLSRPWLDEREEELVLEVLRSGRLSLGPWIDRFEEQ
                     VAERVGAPYAAALSSGTAGLHLLCKIAALGPGDEVITSPLSFVSSANCFILEGATPVF
                     ADVDPVTLNLDPGAVEAAITERTRAIVAVDMFGLPCELDELRAIADRHGLTLIEDACE
                     SLGAEYRGRPLGGHGPSAVFAFYPNKQMATGEGGVVTTHSEEEWRLLRSLRNQGRSYD
                     GGGGWFHHVRLGLNYRWTDVQAAIGLAQLEKLDRIIELREAAAVRYQELLEDVDGVES
                     PSGGDAEHKRSWFVYVVKLEAGLNRGDVIDRLRREGVATAEYVPCIHLQPYMRERYGF
                     APGLCPVAENVSSRTLALPFFTRIDASDQNRVVEVLRTAVADER"
     gene            complement(8710..9111)
                     /locus_tag="VF225_04965"
     CDS             complement(8710..9111)
                     /locus_tag="VF225_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7144640.1"
                     /translation="MNGKVSKSQGDDVTTLLIADDDEDILELVHRGLSASGYEILLAR
                     DGATALATARERNPALVLLDVAMPTPDGYEVTSALKNDPATKDIVVILFTARSEASDV
                     EKGYAAGADDYIMKPFTLKTLQSRVRAALEK"
     gene            complement(9108..>9691)
                     /locus_tag="VF225_04970"
     CDS             complement(9108..>9691)
                     /locus_tag="VF225_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7144641.1"
                     /translation="VVQRNAHRLLGLVSDLLFAAQVQAGRVTLERDLVSVRELLDEAV
                     AAALPTAANREIDVTVSADDVAHVIGDKQRLAQVIDNLLSNALKFTPPNGSVAVSMVA
                     RGATVLIEVADTGLGISPADQKKLFTRFFRTEAAMRKAIKGTGLGLSIVKAIVEGHGG
                     EITVESAEGKGATFRIVLPLAASVPLQPVKEAA"
BASE COUNT         1637 a         3107 c         3303 g         1644 t
ORIGIN      
        1 ggggcccgtg ggggaaacat ggtttccccc acgagacgaa gccgacggct gaggagtttt
       61 tgcatgcctg agtgggaacc cgtcatcggc ctggagatcc acgtccagct gaagacggac
      121 acgaagatgt tctgcggttg cgagtcgggc ttcggaggcg aaccgaacac acgcacctgt
      181 ccggtctgcc ttgctttccc gggagcactg cccgtgccga acgcgactgc ggtggaatgg
      241 acgatcaagc tcggccacgc cctcaactgc cggatcgccg agcgcgccgt cttccaccgc
      301 aagaactact tctatcccga caatcccaag ggctaccaga tctcgcaata cgacgagccg
      361 ctctgcatcg ccggttcctt cgtcgtccca ggccccgaag gggacaccga ggtcggcatc
      421 gtgcgcgctc acctcgaaga ggacgcggcg aagacgattc acgtcggcgg cgagaccggt
      481 cgcatcgtcg ggtcggcgca ctccaacgtg gacttcaacc ggggtggcac gccgcttgtc
      541 gagatcgtga cccagccgga cctccgctcg gcagacgacg cgaagcgctt tctgcagctg
      601 ctgcgccaga ccatcgtcga gctcggcatc tcggacgcgg agatggagaa aggcacgctt
      661 cgcgcagacg cgaatgtctc cgtgcgcgaa gtgggagaga cgggttaccg aacgcgcacc
      721 gaactcaaga acatgaactc gttcaaccac atcgcccgcg ggatcgaagc ggagctccgg
      781 cgccagatcg agacctggga gtccggcggc gaggtcgtcc aggaaacgct cgactacgag
      841 gtgaggtcgg acacggtcac gccgcggaga cggaaggaag aggccgacga ctaccgctac
      901 ttcccggagc ctgatctcgt gcccatcgag ccggaggccg aggtggtcca ccgactacgt
      961 gccgaggtgc ccgagttgcc gggcgtgcgc attcgtcgct tcgagcagca gtacgacttg
     1021 tcgtactacg acgcagaggt gctgaacggc tcggccgccc tcgccgcgct ctacgagcag
     1081 gttgccctcg acggcatcga tgcgaaggcg gcgtcgaacg tcctgatgaa cgacttcgtc
     1141 gcgaccggag tcgatcccgc cgccgtcaac ggcgcggagc tcgcaaagtt gatcgaggcg
     1201 agagcgacga ttcctcgcga gaccttcacg cgtgcgctga ccgagagcgc cgatcctgaa
     1261 ttccgcgcgg agacttatct cggcgacgcg ctcatcgccg acagggcgga gctcgagccg
     1321 cttgtcgagc gcatcctcgc gaccaaccct ggacaggtgg ccgcgtacaa aggcggcaag
     1381 gcgggtctac tcggtttctt cgtcggccag gtgatgaagg agactgaagg caaggccgat
     1441 ccgaaggtcg tcaacgacct tctgcgccaa aaactggcct agtttcgccg acccgcccat
     1501 gggtacccca cggccaactc tctggtcttt cctagcgacc gaaaggagtc gagatggaca
     1561 cgtgggtctg gatcgtgatc gggctggtgg tcgggatcgt cattctcggg atcttgatca
     1621 gcgccctgcg cacgcgacgg tcgcgttcgt tgcgggacag gttcggccga gagtacgacc
     1681 gcacggtcga caagaccggc ggtcgtcgag aggctgagca ggaactcagg aagcgtgagc
     1741 aacgccacga cgagctcgag ctccggccgc tgtcggcgga tgcacgcgag cggtatctcc
     1801 ggcagtggca ggtaacgcaa ggccgcttcg tcgacgaccc gacaggcgcc gtctcagaag
     1861 cggacaacct cgttcaacag gtgatgcgag accgcggcta cccggtggac gacttcgagc
     1921 agcgtgcggc ggacatctcc gtcgagcatc ccgaagtcgt cgagaagtac cggactgcca
     1981 acggcattgc ccgcgcgagt gagcgcggcg aggcttcgac ggaggacctc cggcactcgg
     2041 tacgccatta ccgcgcgctc ttcgtcgagt tgctcgaggt cgacgacgaa gccgaggacg
     2101 cctcaacccg tgacaggcag gtggaggaag tccgtgctca cgacaacgtc gagcgtctgc
     2161 ggtagtggtc tgaccactag atcaagccgc aataccgcgc cgtgttcggc caggcataga
     2221 acccgcggcc ggagcggtgc gcacgctcgg ctgcccacat ctgctcgagc ggcgtccagt
     2281 ggttcgccgt ccccttgcgg gagagcagcc cgccaccgta cgcacgctgg aagctgaggt
     2341 ccatctgaag gccgccgtag tacggcggat tcggatccgt ccaggcgcct tcgaaacggt
     2401 gaatgcagag ccactggcgc ctgtgcggcg gattctgggc tcgctcgcgc gtcttgagcg
     2461 caacccgctg ccagaccgcg atcttcgtct ggacatccgc cggcgggtcg tcgagcttgc
     2521 gggtgagctt gcgccccatc gtgcgttgcc accgccacgt ctcatggcgg tatctgtcga
     2581 tctgacgctg aatgtcacgc tgagtccact gcggttcagc ggtcgtgctt tcaggaggcg
     2641 gctccggggt cgagtccgcg cggatcgtcg cagcgcaggc tagcgccaca ccaagggcgc
     2701 aggcgcacgc gaatgcgagt gtgagtttgg agtgcatctg agtgatcccc ccggatcaac
     2761 gccgcctcgt gactgtgccg cagccgaggt cggctggttg tgtcgaagtc ttaagactgc
     2821 cccgacgggg cagaagttag acatcgctcg tgtcgattgc ctcgaagcgg ccgggtagtg
     2881 agggcgtttg cgaccgaaga gcgcgtcgtt gcggagctcg agctggtgcg tgctgccggg
     2941 tacgcaaggg ggccggcagc tgtacgcaga cgtgcgctcc tacgggttcg acagccacag
     3001 gccgcgctag tctcaaacca gatggccgag aagcgctacc tgctcactcc cgggcctact
     3061 ccggtgcccc cggaggttct tgcagagctc gcgaaaccga tcatccacca ccgcgagcgc
     3121 gacttccgcg agatctacga gcgctgcctc gtgcggctcc aggaggtgta ccggactcgg
     3181 aacgacgtcc tgatgttcac gacctcaggg acgggcgcat tcgagtctgc ggtcgccaac
     3241 ctgacctcgc cgggcgatcg gcagctcgtc ctctcggccg gaaacttcgg cgagcgctgg
     3301 gctggaatgg ctagggcgtt tggcgccgac ctcgtgcacg cgcggctgga gtggggggag
     3361 acacccgagc ccgaggacct gcgctcggcg ctcgccgatg ccggcgacgt gaaggtcgtg
     3421 tacctcacgc attcggagac ctcgaccggc gtggtctgcg acgtgcaggc ccttgcggcc
     3481 gtcgcgaagg acgccggcgc gctcgtggtc gtcgacgcgg tatcgagcct tggagctgtg
     3541 ccgctcgagt ccgatctctg gggagtcgat gtcgtcgtgt ccggctccca gaaggcgctc
     3601 atgtgcccac ccggtctggc tttcgcctct gtctctgccg ctgcgctcgc agcagccacg
     3661 ggcgccggct ctccccgtta cgtgatggac tgggaacgca ctcgcaaagg gcaggcgaag
     3721 ctcgatgcgc cgttcacccc tgccgtttcc cttgttcggg cgctcgacgt cgcgctcggg
     3781 ctgctgctgg acgaagggct cgaggcggcg ttcgatcggc acgcgcggct ggggcgagcg
     3841 tgccgggaag gagcgaaggc aatggggctg gagctgtttt ccccggacga agagcgatcc
     3901 gcggttgtga ccgcgattcg cgccccggac ggcgtcgact cgaccgaggt cgtcaaagca
     3961 ttgcgtgacc gcttcgggat cacgatcgcg aacggccagg ccgatctgaa gggaaagatc
     4021 ttccgcatcg ggcacatcgg ctggttcgac atcttcgaca tcaccaccgc gctggcggcg
     4081 gtcgagctcg tcctggcgga cgcgggcgcg gagatcgaac gtggcgtcgc agtcacccgc
     4141 gcgctcgaag cctgggccga gcacactccc gtttgagcaa ggttctcgtc cgcgaggaga
     4201 tcgcggaggc cggcgttcgc ctcctgcgcg accgtggctt cgaggtcgac gtcgacggtg
     4261 actcggatct cgcggagacg atcgggcgct atgacgcgat catcgtccgc tcggcgacga
     4321 gagtgacagc ggacctgatc gcgcgcgcgg acaacttgaa ggtgatcgga cgcgccggag
     4381 tcgggatcga caacgtcgac gtcgaagcgg cgacccggcg gggcatcgtc gtggcgaacg
     4441 caccggagtc gacggtcatc tctgcggcgg agcacacgat cggcctgctc gtcgcactga
     4501 cgagaaacat cccgcaggcc cacgcagcgc tcaagcaggg ccggtgggag cgcaagacgt
     4561 acggcggcgt ggagctggcc gacaagacgc tcggcgtcct cggcttcggc cggatcggcc
     4621 agcaggtcgc ccgccgtgcc gcggggctcg gcatgcgcgt cgtggcgtac gacccgttcg
     4681 tctcgcgtga ccggtttcgc gagctcggag tagagcgagt cgagaatcag gacgacgtct
     4741 acgcggccgc ggatttcctc acgctccatc tgccgctaac cgacgagacc cgcgaatcgc
     4801 tcaacgcgca cgcgttcggg aagatgcgag acggggtcag gatcgtcaac gctgctcgag
     4861 gcgctctcgt cgacgaggcc gacctgctcg atgcgctgaa gtcaggcaag gtcggcggcg
     4921 ccgcgctcga cgttttttcg accgagccgt attcggggcc gctgctggag ttagacaacg
     4981 tcgtggtcac gccccatctc gccgcctcga ccgaggaggc gcaggatcga gccggtgtga
     5041 tcatcgccga gcaggtggcg gccgccctcc aagggggtct cgtgtcgaat gccgtcaaca
     5101 tccctgtgat cggcgcggag gacctcgaag tgctcggggc gtacatccca ctcgccgcca
     5161 agctcgggcg tctcgtcatg gagctggcag aagggcgcgt cgaggaactg cggctgacct
     5221 acttcggtgc gctggcccag tacgacacca ggctgctgac ggtcgccgct ttgaacggag
     5281 cgttccaggg acgctccgac cagccggtga actacgtcaa cgcgccgatg atcgccgcgg
     5341 agcgcggcgt ggacgtccgc gaggagcgaa gccggtcggc gcgcgactac accaatctcg
     5401 tgcgcgtcga ggccgtcttc ggtggagagc ccctccgcgt ggccggcacg acgatgggca
     5461 acgacaaccg gctctggctc gtcagcgcac tcggcttcga actcgacatg gagctcgcac
     5521 cgctgctcgt ggtcttccgc tacgacgatg ttccgggcgt gatcggcaag gtcgggacgc
     5581 tcttcggcgc ggcgggagtc aacatcgcga acatgacggt gtcgcgaacg cgccagggtg
     5641 ggcaggcact gatggcgctg tcgatcgatt cacccgcgcc gccggaactg gttcagcaga
     5701 tccactcgga gttcgacgat gcccgcttca tctcgctgga gtgagttaca gcgcgtcgcc
     5761 ggcgcgtgga tcgaggcggc gaacggttcg cgccacgacc gagttgaact tctccacgat
     5821 gacgaagcgc ttgtgcgggc ttttcacgac ccgttcgacc tcgggtacct cgtgaccggc
     5881 gtgcagcagg aagtgcgtcc cgtcggaacg gacctcctcg tactgatcga gtgtcgcggg
     5941 aactctttcg gcgcactcgg gatcagcgca ttcgcagacg aactcggcgt cgacggcgtc
     6001 gaaccggtcg gcgctttcgg cgattctctc gttgacgtct ctgaacagcg cctcggtgtg
     6061 cgcgatccgc tcttcgcgct cggccatcgt cgtctcccgt cgattgactg tcagttgaag
     6121 aacggcgaag gcgtcagatc ggttgcgcca cgtgtggcct gaggcggtag agcgcattgc
     6181 ggcgttcctg cgagtctcgg gcgtcgaggg gaggctcgag gagctgctgc ccggggctgg
     6241 gccgccgccg ggccagaagc tgtcggcaaa agggttcgat ggaaacggcg ctgcgatggt
     6301 cgcgatcgtt cccgtgggcc gcagcgtcga cgagcggaag ctcgccgcgg cggcgggctg
     6361 cgacgaggta cagcccgctg ccgcgaccgt ctttcccttc gcggaagacc gcgtcttcat
     6421 ggatcagtcg atcctgtcct tcgacgccgt ctggttggag gcgggctcgt cgcgtcacat
     6481 cctcggattg actccggtcc agctcgcccg tgtgacgaac gcggaaaccg cagatctcgt
     6541 tcgggaaggc tagaaggggg aggtcgacga cgtgcggcgc gggtagaatt cgcctccggt
     6601 cgcccccaaa aggagaaacc tcttcatgca ggaactcgag aagatttgga tgaacggcga
     6661 gctcgtggac tgggccgacg cgaagatcca cgtcgggtcg cacggcctcc actacggctc
     6721 cggcgtgttc gagggaatcc gcgcgtacga gacgccgaaa ggcagtgccg tcttccgact
     6781 gaccgagcac ctggagcgcc tccagagctc cgcgcaggtc ctcaacatgg agctcccata
     6841 cacggtggcg gagcttcgat ccgccacgat ggagctcatc ggcgcgaacg gtgtagccga
     6901 gtgctacatc aggccgattg ccttcttcgg gttcggggag ctaggcgtcg cttcgaccgg
     6961 caatcctgtc gacgtcgtga tcatgagctg gccctggggc acgtacctcg ggcccgaggc
     7021 attgaagaac ggcatccgcg cgaagatctc gtcctggact cgggtcgggc cgaatgtcat
     7081 cccgcacgtc gcgaaggcaa cgggcatcta cctgaactcg atgctcgcgg tgatggaggc
     7141 gaaccgtgct ggttacgacg aggcgatcct gctgaccgac gacggctaca tcgccgacgg
     7201 ttccggggag aatgtcttca tcgtcaagga cgggacgatc tatacgcctg atctctcggc
     7261 gtcgatcctg cgcggcatca cgcgcgacac gatcatccag atcgctcagg acctcggtca
     7321 ctccgtcgtg gagaagcagc tgatccgtac cgatctctat ctggccgacg aggtcttcat
     7381 gacggggact gccgccgaag tgactccgct gcgcgaggtc gacgatcaga cgatcgggcc
     7441 gcctgggccc gtgactctgg aaattcagaa ggcgtacctc gacacggtgc gcggtggcag
     7501 cgagcgctgg tcgcactggc tcgaatacgc ctcgaccgaa ccggcgcgag cggaggcatg
     7561 accgaagcag tccgggagat cccactctcc cggccgtggc tcgacgagcg tgaggaggag
     7621 ctcgtcctcg aggtgctgcg gtccgggcgc ctgtcgctcg gaccctggat cgaccgcttc
     7681 gaggagcagg ttgccgagcg ggtcggcgcg ccctacgcgg cggcgctctc cagcggcacg
     7741 gccggactgc acctgctctg caagatcgca gcgctcggac ccggcgacga ggtgatcacg
     7801 tcaccgctgt cgttcgtctc ctcggcgaac tgcttcatcc tcgagggtgc gacacccgtc
     7861 ttcgccgacg tcgacccggt gacgctcaat ctcgatccgg gtgcggtcga agcggcgatc
     7921 acggaacgga cccgcgccat cgtggcggtc gacatgttcg ggctgccgtg cgagctcgac
     7981 gagctgcggg cgatcgccga ccgccacggc ctgaccctga tcgaggacgc gtgcgagtcg
     8041 ctgggcgcgg agtatcgcgg tcgtccgctc ggcgggcatg ggccttcggc ggtcttcgcg
     8101 ttctatccga acaagcagat ggcaaccggc gaaggcgggg tcgtcacgac tcattcggag
     8161 gaggagtggc ggttgctccg cagcctcagg aaccaggggc gttcctacga cggaggcggc
     8221 gggtggtttc atcacgtccg gctcggcctg aactatcgct ggacggatgt gcaggctgcg
     8281 atcggcctgg cccagctgga gaagctcgac cggatcatcg agctccgtga ggcagcggcc
     8341 gtccggtacc aggagctgct cgaggacgtg gacggtgtcg agagcccatc cggcggggat
     8401 gccgagcaca agcgttcgtg gttcgtctat gtggtcaagc tcgaagccgg gctgaatcgc
     8461 ggcgacgtga tcgacaggct caggcgggag ggagtcgcga cggccgagta cgtgccatgc
     8521 atccacctgc agccgtacat gcgcgagcgg tatgggtttg cgccgggcct ttgccccgtc
     8581 gccgagaacg tttcgagccg aacgctcgcg ctgccgttct tcacccggat cgacgcctcc
     8641 gaccagaacc gagtcgtcga ggtgctccgc accgcagtcg cagacgaacg ctagtacggc
     8701 gcgggtcgcc tatttctcga gcgcggctcg gacgcggctc tgcagcgtct tgagcgtgaa
     8761 cggtttcatg atgtagtcgt cggcgcccgc tgcgtagcct ttctcaacgt ccgacgcttc
     8821 ggagcgggcg gtgaagagga tcacgacgat gtccttcgtc gcgggatcgt tcttcagcgc
     8881 agacgtcacc tcgtacccgt ccggggtcgg catggcgacg tcgagcaaga ccaacgccgg
     8941 gttccgctca cgcgccgtgg caagcgcagt cgcgccgtcg cgggcgagga ggatctcgta
     9001 gccgctcgca gagagcccgc ggtgcacgag ctccagaatg tcctcgtcgt cgtccgcaat
     9061 caggagcgtc gtcacgtcgt ctccctgaga cttgctcacc ttgccgttca tgctgcctcc
     9121 ttgaccggtt gaagaggaac gctcgcggcg agtggcagaa cgatcctgaa cgtcgcgccc
     9181 ttgccttccg cactctcgac cgtgatctcg ccgccgtgac cctccacgat cgccttgacg
     9241 atggagagac cgaggccggt ccccttgatt gccttccgca tcgctgcttc ggtgcggaag
     9301 aagcgggtga acagcttctt ctgatccgca ggcgagatgc cgagccccgt gtctgccacc
     9361 tcgatcagga cagtggcccc ccgagcgacc atggagactg cgaccgagcc gttcggaggc
     9421 gtgaacttga gcgcattcga aaggaggttg tcgatcacct gtgccagccg ctgcttgtcc
     9481 ccgatcacgt gggcgacgtc gtccgcgctt acagtcacgt cgatctcacg gttagccgcc
     9541 gttgggagcg cagccgcgac ggcctcgtcg aggagctcgc gcacgctcac gagatccctt
     9601 tcgagcgtta cgcggcccgc ctgcacctgc gccgcgaaga gcaagtccga gacgaggccc
     9661 aggagccgat gcgcgttgcg ttggacaacc t
//
LOCUS       DASVEU010000120         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1050956,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000120 DASVEU010000000
VERSION     DASVEU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050956"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..1471)
                     /locus_tag="VF225_04975"
     CDS             complement(443..1471)
                     /locus_tag="VF225_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7144642.1"
                     /translation="MSTLGRLLSPFRLASAGFIVLVATVAILVTRGSDQYIEIPDDAH
                     PLANLVQVPGAKPDDDGGGIYYVDVLLKPASLLESYVKFVRPEGSDLIPRAQIVQPGI
                     SDQERFELDLATMKASQSIASVVALRELGIRVPIRPDGVRVVAVTSDSHAAGVLRPGD
                     VIVAADGNPVLTRTDLAATLARLKPGAQVSITIRRGGKKLTFPIRTTADTQNPGRTII
                     GVLPIQALGVRLPFPIKFNLQKVGGPSAGLAFALELLEKKGRDVDHGYKVAATGEMQL
                     NGSVTRIGGIKQKTIGARKSHVDVFLVPVDGDNARDAKRYAGGLRIIPVKSFQQALQA
                     LATLPPKS"
     gene            complement(1475..2218)
                     /locus_tag="VF225_04980"
     CDS             complement(1475..2218)
                     /locus_tag="VF225_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7144643.1"
                     /translation="MDLGLRGRTAIVSGASAGLGLATAEALAGEGANVTMLARRRDVL
                     QREADRIGALAVRGDVTNPRDLEAVVRQTVAAFGGIDILVWNSGGPPPGPAVGMSAES
                     LEEAVELLFFPALRLVDLCLPYLVQSAGGRILVFTSVAAKEPTDHLALSNAVRPGVHG
                     WAKTLARELGPRGVTVNCLAPGRIETARLAQLYPEGPTEADLKAIPVGRWGTPQEFGD
                     VACFLASDRARYVNGATVVVDGGLSRGLL"
     gene            complement(2221..3144)
                     /gene="mdh"
                     /locus_tag="VF225_04985"
     CDS             complement(2221..3144)
                     /gene="mdh"
                     /locus_tag="VF225_04985"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7144644.1"
                     /translation="MRRKVTVVGAGNVGATCAQVLAARDYANVVLVDIKEGLPQGKAL
                     DINQMGAVLGYEPRVTGSNSYGETAGSDVVVITAGLPRSPGMSRDDLVATNEKIVKDV
                     TENAIAQSPDAILIVVSNPLDAMCHVAKSVSGWRKERVFGMAGILDTARFSTFIAWET
                     QASVKDVTAMVLGGHGDQMVPAVSATTVGGIPLAELVGAADIDAMVQRTAKGGGELVE
                     LLGTSAWYAPGAAAAQMVESIMLDEKRVLPCTAYLEGEYGIDGLYMGVPVKLGSGGIE
                     AIYELDLNEDEQAMLNESAGAVREVVGLLTS"
     gene            complement(3141..>3754)
                     /locus_tag="VF225_04990"
     CDS             complement(3141..>3754)
                     /locus_tag="VF225_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7144645.1"
                     /translation="KPISITGTRRIVEFAFDYARRNGRHKVTAVHKANIMKFSDGLFL
                     RVAQEVAAENDDIEFNDRIVDNMCMQLVQRPEEYDVLVLPNLYGDVLSDLCAGMIGGL
                     GLAPGGNFGESIAIFEPTHGSAPKYAGQNKVNPMAQILSGMLMLRHLDERAAADNLER
                     AIAEVIREGKSVTYDMKPTRSDPSAVGTSQVADAIIEKLGVRV"
BASE COUNT          596 a         1240 c         1257 g          661 t
ORIGIN      
        1 tcggaggtgc gccgaacacg gcgcggctcg cattcagctt cgtctctccg gacgagatca
       61 gggaaggcgt gcgccggctc gcagccctcg tccgggaacc cgcgccggtt tagcgcctag
      121 cccgctgccg ctacttgcgc gaagtcgccg agcggcctcg gtacgaggcg gggctggcgg
      181 ggtggaacga gtgacccccg gctattgagc cggaaggtgg ggcagggcgc gtccgtcgga
      241 agggcacaga gcccggcgcg acggaagtag cagtcgtcgc acgtcagttg gttccgctta
      301 ctcaattttc tcccctgcct agagattagg gccgcagtga caagcggtga tactacgaac
      361 acggctggcg tagacaaggg ttgccgcaca ggaaaactaa gcattttccg gcagattcga
      421 tcttgccgaa aactgctgtg ttttagctct tcggcggcag cgttgccagt gcctgcaacg
      481 cctgttgaaa actcttcaca gggatgatcc tcaggccacc cgcgtagcgc ttcgcgtcgc
      541 gtgcgttgtc cccatcgacc ggcaccagga agacgtcgac atgcgacttt cgagccccga
      601 tcgtcttttg cttgataccg ccgatcctcg tcaccgatcc gttcagctgc atctcgcctg
      661 tagcggcgac cttgtagccg tggtcgacgt cgcgtccctt cttctccaac agctcgagtg
      721 cgaacgcgag accggcagac ggcccgccga ccttctggag gttgaacttg atcgggaacg
      781 gcaggcggac gcccagtgcc tggatcggca ggacgccgat gatcgtccgc cctggattct
      841 gggtgtcggc ggtcgttcgg atggggaacg tgagtttttt cccgccacgc cggatggtga
      901 tgcttacctg ggccccgggc ttgagccgag cgagcgtcgc ggcgagatcg gttcgcgtga
      961 gcaccgggtt gccgtcggcc gcaacgatga cgtcgccggg ccgcaacacg ccggcggcgt
     1021 gagagtccga ggtcacggct acgacgcgaa cgccgtcggg gcggatggga acgcgtatgc
     1081 cgagctcgcg cagcgcgacg accgaggcga tcgactggga cgccttcatg gtggcaaggt
     1141 cgagctcgaa gcgttcctga tctgagattc caggctgcac gatttgcgct cgcgggatca
     1201 ggtcggagcc ctcggggcgg acgaacttga cgtaggactc gagcaacgac gccggcttga
     1261 gcaggacgtc gacgtagtag atcccgcctc cgtcatcgtc cggttttgcg cccggcacct
     1321 gcacaaggtt ggccagcgga tgagcgtcgt cagggatctc gatgtattgg tcgctcccgc
     1381 gcgtcaccag gatggcgact gtcgcgacga gcacgatgaa cccagccgag gcgagccgga
     1441 acggcgacag caaccggcct agcgtggaca cttactagag caggccccgt gagaggccgc
     1501 cgtcgacgac caccgtcgct ccgttcacgt aacgggcgcg gtcggaggcg aggaagcagg
     1561 cgacgtcgcc gaactcctgc ggcgtgcccc agcgtcccac cggaatcgct ttcaggtcgg
     1621 cttcggtcgg tccttccggg tacagctgcg cgaggcgcgc ggtctcgatc cggcctggcg
     1681 cgaggcaatt cacggtgacc ccgcgagggc cgagctcgcg ggcaagcgtc tttgcccagc
     1741 cgtggacacc ggggcgcacg gcgttgctga gcgcgagatg gtcggtcggc tctttcgccg
     1801 ccaccgaggt gaagacgagg atgcgaccgc ccgcgctttg caccagatag ggcaggcaga
     1861 ggtcgacgag gcgcagggcc gggaagaaga gcagctcgac agcttcctcg agtgattcgg
     1921 cgctcatccc gacggccggt cccggcggcg ggccgccgct gttccacacg aggatgtcga
     1981 tgccgccgaa cgcggcaacg gtttgccgca cgactgcctc gagatcgcgc gggttcgtga
     2041 cgtcaccccg cacggcgagc gcaccgatcc ggtcggcttc gcgctgcagc acgtcacgcc
     2101 gccgcgcgag catggtcacg ttcgcgcctt cgcccgccag agcctcggcc gtcgcgaggc
     2161 cgagaccggc cgatgcgccg ctcacgatcg ccgtgcggcc tctgagaccg agatccatgg
     2221 ttacgaggtg agcaagccga cgacttcgcg cacggcgccg gccgactcgt tcagcattgc
     2281 ctgctcgtcc tcattcaggt cgagctcgta gatcgcttcg atcccgccgg atccgagctt
     2341 gaccggcacg cccatgtaga ggccgtcgat gccgtactcg ccctcgaggt aggccgtgca
     2401 cggaagcacg cgcttctcgt ccagcatgat cgactcgacc atctgcgcgg cggcggcgcc
     2461 gggcgcgtac caggccgacg tccccagcag ctcgacgagc tctccgccgc cctttgcagt
     2521 gcgttgcacc atcgcgtcga tgtcggctgc gccgacgagc tctgcaagtg ggatcccgcc
     2581 taccgtggtc gcgctgacgg ccggaaccat ctggtcaccg tgccccccca ggaccatcgc
     2641 ggtgacgtcc ttgacggacg cctgcgtttc ccaggcgatg aacgtcgaga agcgtgcggt
     2701 gtcgaggatc ccggccattc cgaatacccg ctccttccgc catccggaaa cgctcttcgc
     2761 cacgtgacac atggcgtcga ggggattgga gacgacgatc aggatcgcgt ccgggctctg
     2821 cgcgatcgcg ttctcggtca cgtccttgac gatcttctcg ttcgtcgcga cgaggtcgtc
     2881 gcggctcatt ccgggcgagc gcggcaaacc tgccgtgatg acgacgacgt cggagccggc
     2941 ggtctcgccg tagctgttcg agcctgtcac ccggggctcg tacccgagca ccgcgcccat
     3001 ctggttgatg tccagagcct tcccctgcgg gaggccttcc ttgatgtcga cgagaacgac
     3061 gttcgcgtag tcgcgcgccg ccaggacctg ggcgcaggtg gcgccgacgt tccctgcgcc
     3121 gaccacggtc accttgcgcc tcatacgcgt actccgagct tctcgatgat cgcgtcggcg
     3181 acctggctgg tgccgacggc gctcgggtcg gagcgcgtcg gcttcatgtc gtacgtgacg
     3241 ctcttgccct cccggatgac ctcggcgatc gcccgctcga ggttgtccgc ggccgcacgc
     3301 tcgtcgaggt gacggagcat caacattcca gagaggatct gcgccatcgg gttgaccttg
     3361 ttctggcccg cgtacttcgg cgcggagccg tgcgtcggct cgaagatcgc gatgctctcg
     3421 ccgaagttcc cgccgggggc gagcccgagc ccgccgatca ttccggcgca gagatcggag
     3481 agcacgtcgc cgtagaggtt cggaagcacg agcacgtcgt actcctccgg gcgctggacg
     3541 agctgcatgc acatgttgtc cacgatgcgg tcgttgaact cgatgtcgtc gttctcggcg
     3601 gcgacctctt gtgcgactcg gaggaacagg ccgtcagaga acttcatgat gttcgccttg
     3661 tgcaccgccg tgaccttgtg gcgaccgttg cgccgcgcgt agtcgaaggc gaactccacg
     3721 atccgccgcg tgcccgtgat agagatcggc ttga
//
LOCUS       DASVEU010000121         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_470675,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000121 DASVEU010000000
VERSION     DASVEU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470675"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..>2302)
                     /locus_tag="VF225_04995"
     CDS             complement(40..>2302)
                     /locus_tag="VF225_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7144646.1"
                     /translation="VAVNHILLAVMLRLARGFTLRETGLFEIEYVATDLVLAALGFAV
                     YAFWQSNPWLIPFALTPLFLIHRSLSVPALQAEARVDPKTGLFNARHFAAALAEEIGR
                     ARRFERPMSLIMADLDLLRDINNTYGHLAGDAVLKGIAEAFRAQLRHYDVPARFGGEE
                     FSILLPETPPEQALEIAERIRRAVAERTFDVETSSEPIRATVSIGVAGFPKDGADANE
                     LIHQADLAVYRAKLQGRNRVLGASSEPLLVPADRSTRLIAVPEDGDHHAPLAAATQVQ
                     PLEERREVRHPRPHAVHGPRFLSLDRRLSLIVGLVSAVGVGAGIIGLAFSWTSKDLVG
                     LLTVVGLVGVGQALALEADEGSAVLSVSAVGCLAAASLFGFRAALPLALASVAVEWSA
                     RRAPLHNVLFNVGALTLASLAAASVFKIAAFFPNGTAHELAASGLGLLAGTAYFVVNM
                     GLVSLALALQGHERWWSVFRERFAWLLPHYLVYGFIGGVMALAYEAVQLLALAVFAVP
                     LLLMRKTQEAYLKHTQRSAQKLRQAAETIQSQNVSLELANKLLKERSTAAMESLSATV
                     DARDSYTAGHSRRVQQLALAIGRELGLSHAELDLLGHAALFHDIGKLAIPDAILLKPA
                     SLTPDEWSLMQRHAEEGARIIDRLGFLNDAVPAIRHHHERFDGTGYPDRLQGEEIPLG
                     ARIIHVADALDSMLTTRIYRAARPAAEALQELRRAAGTQFCPRCVSALERILPLETIQ
                     SDADRARLSLVAS"
BASE COUNT          385 a          733 c          841 g          343 t
ORIGIN      
        1 gttctggaaa gaacgcaagt taaggcttct tgatccctcc taggaagcta cgagggagag
       61 gcgagccctg tccgcgtcgc tctggatcgt ctcgagcggc aggatccgct ccagggcgct
      121 gacgcagcgc gggcagaact gggtgccggc tgcacggcgg agctcctgaa gggcctctgc
      181 ggccggccgg gcggcgcggt aaatgcgggt cgtcagcatc gagtcgagcg cgtcggcaac
      241 gtggatgatc cgcgccccga gcgggatctc ctctccctgt agccggtccg ggtaccccgt
      301 gccgtcgaag cgctcgtggt ggtggcggat ggcgggcacg gcgtcgttga ggaagccgag
      361 gcggtcgatg atgcgcgccc cctcctccgc gtgccgctgc atcaacgacc actcgtcagg
      421 cgtgaggctc gcgggcttca gcaggatggc gtcggggatc gcgagcttgc cgatgtcgtg
      481 gaagagcgcc gcatgcccga gcaggtcgag ctccgcgtgc gagagcccga gctcccggcc
      541 gattgcgagg gcaagctgct ggactcgacg cgagtggcct gctgtgtagg agtcccgcgc
      601 gtccacggtc gccgagagtg attccatcgc cgccgtcgag cgctccttga gcagcttgtt
      661 ggccagctcg agcgagacgt tctgcgactg gatcgtctct gcggcttggc ggagcttctg
      721 ggcactgcgt tgcgtgtgct tgaggtacgc ctcctgcgtc ttgcgcatca gcaggagcgg
      781 caccgcgaac accgcgagcg cgagcagctg gaccgcctcg taggcgagcg ccatcacgcc
      841 gccgatgaag ccgtacacga ggtagtgcgg cagcagccag gcgaagcgct ccctgaagac
      901 cgaccaccaa cgctcgtgtc cctggagtgc cagtgcgagc gagacgagac ccatgttgac
      961 gacgaagtag gcggtaccgg cgagcagtcc gaggccggag gcggcgagct cgtgcgcggt
     1021 gccgttcggg aagaacgcag cgatcttgaa cacgctcgcc gcggcgagcg atgccagcgt
     1081 cagcgcgccg acgttgaaga gcacgttgtg cagcggcgct cgccgcgcgc tccactcgac
     1141 tgcgacgctg gcgagcgcaa gaggcaaggc ggcgcggaag ccgaagagcg aggcggctgc
     1201 gagacagccg acggcgctga ccgagagcac agcagatcct tcgtctgcct cgagcgcgag
     1261 agcctgcccc actccgacca gcccgacgac ggtcaggagg ccaaccaggt ccttcgaggt
     1321 ccagctgaag gcgaggccga tgatcccggc ccccacgccg actgcgctga cgaggccgac
     1381 gatcaggctc agccggcggt cgagcgagag gaagcgcgga ccgtggaccg cgtgtgggcg
     1441 cggatggcgc acctcgcggc gttcctcgag gggctggacc tgggtcgcgg ccgcaagcgg
     1501 tgcgtgatgg tccccgtcct ctggcacggc gatcaagcgc gtcgagcgat ccgcgggcac
     1561 gagcagcggt tcggagctcg ctccgaggac gcggttacgt ccctgcagct tcgcgcggta
     1621 gacggcgagg tcagcctggt ggatgagctc gttcgcgtcg gcgccgtcct tcgggaagcc
     1681 ggcgactccg atcgagacgg tcgcacggat cggctcgctc gaggtctcga cgtcgaacgt
     1741 gcgctcggcg acggcgcggc ggatccgttc cgcgatctcc agtgcctgct cgggaggtgt
     1801 ctccggcagg agaatcgaga attcctcccc gccgaaacgt gccggcacgt cgtaatggcg
     1861 gagctgggca cggaaggctt cggcgatccc cttcagcacg gcgtcgcccg caaggtgacc
     1921 gtaagtgttg ttgatgtccc gcaagaggtc gaggtcggcc atgatcagcg acatcgggcg
     1981 ctcgaagcgg cgggcgcggc cgatttcctc cgccagcgcg gcggcgaaat ggcgcgcgtt
     2041 gaagagcccc gtcttcgggt cgactcgcgc ctccgcctgg agtgccggaa cgctcagcga
     2101 gcggtggatc aggaacagcg gtgtgagcgc gaagggaatg agccacgggt tcgactgcca
     2161 gaaggcatag acggcgaacc cgagcgctgc gagcacgaga tccgtggcga cgtactcgat
     2221 ctcgaagagt cccgtctcac gcagtgtgaa cccacgagcc agcctcagca tcaccgccag
     2281 gagaatgtga ttgacggcga cg
//
LOCUS       DASVEU010000122         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1636556,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000122 DASVEU010000000
VERSION     DASVEU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636556"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1685
                     /locus_tag="VF225_05000"
     CDS             48..1685
                     /locus_tag="VF225_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7144647.1"
                     /translation="MQTTDDLERGREAYERSAWSEAYELLGRAERAAPLSGSDLVSLS
                     IAAHMLGRVDEWIPLLERAHLAYAKEGEALPAVRCAFWIGMNLALRGEMAPAAGWLGR
                     AQRLLEREEGESVEQGYMLLPVAFQHEVAGDFDGAAATAAAAAEIGERFGDVDLFALA
                     VHVQGTVLAKGGRVAEGLSLLDEAMVAVTAGEVSPVVSGIVYCGVILACEEVYELRRA
                     HEWTAALTRWCEQQPDLVSFRGRCLVHRAQLLRLHGDWPAALEEARLAGERFAEAMNP
                     GAIAKGWYLQGEVQRLSGRFEEAEQAYREASRLGLEPQPGLALLRLAQGKDEAAAAAI
                     RRAEGETTDRAARAGLLPTYVEIMLAAGDVDAARVACAELREIAAQYGTDMLQASLAQ
                     ACGAFELAAGDAGSALISLRRAVQAWQELEAPYEVALTRVLVGRACRALGDEDGFALE
                     LDAARRLFEQLGAAPSVASVDSLTRADERDGTHGLTSRELEVLRLVATGKTNREIASV
                     LVISEHTVARHVQNIFIKLGVASRTAAGAFAFEHELV"
     gene            1809..>2002
                     /locus_tag="VF225_05005"
     CDS             1809..>2002
                     /locus_tag="VF225_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7144648.1"
                     /translation="MATQEAALDSNSEAVASTETFDTIVIGGGQAGLAVGYYLMKHGG
                     SFVILDANEQIGGSWRTRTW"
BASE COUNT          322 a          621 c          732 g          327 t
ORIGIN      
        1 cgcaacttaa ctttacgatc ggcttggttt ggaggacatt gggtctcttg cagaccaccg
       61 acgacctgga acgtggccgc gaggcatacg agagatccgc ctggagcgag gcttacgagc
      121 tgctgggacg cgccgagcgc gcggctcccc tctcgggctc ggacctcgtg tcgctgtcca
      181 tagcggcgca catgctcggc cgcgtcgacg aatggatccc gttgctggag cgcgcgcacc
      241 ttgcgtacgc aaaagagggc gaggcgctac ctgcggttcg ctgtgcgttc tggatcggga
      301 tgaaccttgc gctacgggga gagatggcgc cggccgccgg ttggctcggc cgcgctcaac
      361 ggttgctcga gcgcgaagag ggcgagtccg tcgagcaggg ttacatgctc ctgccagtcg
      421 cctttcagca cgaggtggct ggtgacttcg atggtgcggc cgctaccgcc gccgcagcgg
      481 ccgagatcgg cgagcgcttc ggcgacgtcg acctcttcgc gctcgccgtg cacgttcaag
      541 ggaccgtgct cgcgaaaggc ggccgcgtag ccgagggcct cagtctgctc gacgaggcga
      601 tggtggcagt gactgcggga gaggtgtctc ccgtcgtcag cggcatcgtg tattgcggcg
      661 tcattctcgc gtgcgaagag gtctacgagc tgcgccgcgc ccacgagtgg acggcagcac
      721 tgacacgctg gtgcgaacag cagccggacc tggtctcgtt ccgagggcgc tgcctcgtgc
      781 atcgcgccca actcctgcgg ttgcacgggg actggcccgc agcgctggag gaggcgcgcc
      841 tggcgggcga gcgcttcgca gaggcgatga atccgggagc gattgcgaaa gggtggtacc
      901 tgcaaggcga ggtccagcga ctgagcggcc ggttcgagga ggctgagcag gcgtaccggg
      961 aagcgagccg tctcggtctg gaaccgcaac ctggacttgc gctcctccgg cttgcccagg
     1021 gcaaggatga ggcggcggcg gcggcgattc ggcgggcgga gggcgagacg actgatcgcg
     1081 cggctcgcgc gggtctgttg ccgacctacg tcgagatcat gctcgcggcc ggcgacgtcg
     1141 acgcggcgcg cgtagcctgc gctgagctgc gcgagatcgc ggctcaatac gggacagaca
     1201 tgctccaggc ctcccttgcg caggcttgcg gcgcattcga gctcgccgcc ggcgatgcgg
     1261 ggagcgccct gatcagtctg cgtcgggcgg tccaggcctg gcaggagctg gaggctccct
     1321 acgaggtagc gctcacgcgt gtcctcgtgg ggcgagcgtg ccgtgcgctc ggtgacgaag
     1381 atggattcgc cctcgagctg gacgcggcgc ggaggctctt cgagcagctc ggcgcagcgc
     1441 cctccgtcgc gtcggtggac tcgctcacga gagccgacga gcgcgacgga acgcatggat
     1501 tgacttcgcg tgagctggaa gtgctgcgac tggtggcgac cgggaagacc aaccgcgaga
     1561 tcgcgtcggt gttggtgatc agcgagcaca ccgtcgcacg gcacgtgcag aacatcttca
     1621 tcaagctcgg cgtcgcgtcg cggacggcgg cgggcgcgtt cgcattcgag cacgagctcg
     1681 tctgacgcgt cgcatggtca gaaatgacca tgcggccgcc gtggcaagtt ggtggatccg
     1741 cgcgatgcgc gctcttcgcc tccgccctaa cgtcgtcgtc aaggtcattc gacagtggag
     1801 gaacgatcat ggcgacacag gaagcagctc tcgacagcaa cagcgaagca gtcgcgagca
     1861 ccgagacatt cgacacgatc gtcatcggcg gcggccaggc cggcctcgcg gtcggctact
     1921 acctgatgaa gcacggcggc tcattcgtga tcctcgacgc gaacgagcag atcggcggtt
     1981 cctggcggac gcgcacctgg ga
//
LOCUS       DASVEU010000123         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1442458,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000123 DASVEU010000000
VERSION     DASVEU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442458"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VF225_05010"
     CDS             complement(<1..286)
                     /locus_tag="VF225_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144649.1"
                     /translation="MASEVVDLFTELAALPSPPGDERAVADVVIGYLRDLGLSVDEDD
                     AGAKVGSNIGNVYCRLEATDGGSPIFLCAHLDTVPPEGPIEPVIEEGVVRN"
     gene            complement(309..1961)
                     /locus_tag="VF225_05015"
     CDS             complement(309..1961)
                     /locus_tag="VF225_05015"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7144650.1"
                     /translation="MSSPASPTKYVFVTGGVVSALGKGIVAASLGRLLKARGLRVQAQ
                     KFDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDIGHYERFIDENLTRAASHTAGAIWD
                     SVLRKERKGEFLGATVQVIPHITNEIKHRIRASAVSTPTDVVISEIGGTVGDIESLPF
                     LEAIRQFRREVGAENVLYLHVTLVPFLEAAGELKTKPTQHSVNELRRIGIHPDIVVCR
                     SHEELSADIREKIALFADVEPEAVIASPDVPDVYLVPSRLKAEGLDRLVGEKLGLPAT
                     EADLGDWLDLNERMQQSEEIVEIALVGKYVKLQDAYLSVHEALKHAGAHHGAKVRVSW
                     VDAEGMSLEEATGLLEQVDGVLVPGGFGSRGWEGKILACRVAREREIPYLGICLGMHV
                     AVSEFSRHVLGLDGANSTEMDPETPHPVIDLLPEQKEIEDLGGTMRLGAQAVEVVEGT
                     RAYDAYGEPVVHERHRHRYEVNNHYRPALVDGGLLVSGTFQAGRLVEIVELPDHPWFV
                     ASQFHPEFKSRPTRPAPLFRDFVGAALVRARERSVVGAASTV"
     gene            complement(1958..3166)
                     /locus_tag="VF225_05020"
     CDS             complement(1958..3166)
                     /locus_tag="VF225_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7144651.1"
                     /translation="MDEPLTILVPARDEEAAIGSTVSRLRKAFPEGEVIVADDGSRDR
                     TAEVAEEAGALVLRLPRRGKGQALSAAERAAPPGALLLCDADLSGELEPLLRGDGDLN
                     IAAFAEQVGGGFGLAKRVARGLIEVLGGLDVREPLSGQRALSAAARAACFPVAAGFGC
                     EVRMTIDAARAGLRVFEVELPLGHRSTGRDLSGFVHRARQLRDTVYACGPLGINFRGL
                     RLPLVGWKVGATGGPAAAAVGALGLADDLWSGPERGFGAHLRAGRTTGVLKLVGIPAV
                     GLLATRRVSGALLVGLAANVLNQLDTRPGRALKAYLAAALPLDAPVGVAVLLLPYDLR
                     EMAMLGDAGSNALGALLGLNSVKRFTGRGQWVAISALASLTILGERKSIGAWIERAPV
                     LSWIDRLGRV"
     gene            complement(3159..4049)
                     /locus_tag="VF225_05025"
     CDS             complement(3159..4049)
                     /locus_tag="VF225_05025"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX7144652.1"
                     /translation="MFDFRYHVASLAAVFFALVIGILVGVALASHGLGNTERKRLEED
                     LRRAESQADVLKTQVATLMNEGAGNRAFVEGTYKSVMTNRLKGKKIAVLFIGSSEKAR
                     RTEITQALADAGAGTPLRMRFVQVPIDPAALARKLATKPLLASYSAPDQLQNLGHDLG
                     QEFVAGTDTPLWIALQSLIVEEKAGPSKPPADGVVIVRTATSQTGPTALFLKGLYAGL
                     ADGGVPVVGVERTNDPGSAIRAFQRAGFSTVDDIETRIGKLALVALLADPTSTGAFGT
                     KKTAQAQLPPISPLTATAGG"
     gene            complement(4068..4490)
                     /locus_tag="VF225_05030"
     CDS             complement(4068..4490)
                     /locus_tag="VF225_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7144653.1"
                     /translation="MARAAASRELLAAREDVWSFVAEPHHFADWWPGIGGVQPDRRGF
                     AEGARWGISGTDRPTLLRRATSSGMLLVTGIETPKRFAWRLTGDHIDAELRLDERGAD
                     RTIATLNIEAPWLYGFSRALPRRALTRLHALCQTAAEL"
     gene            complement(4506..5639)
                     /gene="steA"
                     /locus_tag="VF225_05035"
     CDS             complement(4506..5639)
                     /gene="steA"
                     /locus_tag="VF225_05035"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX7144654.1"
                     /translation="MSFVELTGHAKLDRRTKNLVKRLSADDIAIIDHTDLDRVSAEAL
                     LESGVRVVVNVAPSQSGRFPNPGPLALVRGGIRLIDVTADGLFEHVNEGELLTVRGAG
                     LYRNGTCLATGQVLEERDLERKLAEQRGRVTEALEAFAENTMRYLREEGKLLAEGIEF
                     PPLKTGFRDRHALVVARGPGYKRDLRMVKPYIRAFKPVLIGVDGGSDALLEAGYKPDV
                     IVGDMDSVSDKALRSGAEVLVHGYLDGVAPGEERAAQLGVKYEVVRATGVSEDVALLL
                     AQEKGSELIVAVGTHFNLIEFLERNRAGMSSTFVTRLKVGEILIDAKGVSRLASRQVG
                     IWPLVLFTLAGLGAIAVAILVSPALRHLLEYLAQRLRDLLGIG"
     gene            complement(5636..>6053)
                     /locus_tag="VF225_05040"
     CDS             complement(5636..>6053)
                     /locus_tag="VF225_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX7144655.1"
                     /translation="DPGPTGVDEAVFLIRPNAGLPFGPVAETASGGELSRVALAIAAV
                     GGGETLVFDEIDAGIGGETAHAVAEALRALAGRAQVVTITHLPQIASVADRHFRVEKI
                     AGDPTHTRINLLDDAERRDELQRMLGGREFLSSVQK"
BASE COUNT         1004 a         2085 c         1985 g          979 t
ORIGIN      
        1 cgttgcgcac gacaccctcc tcgatcaccg gctcgatcgg gccttcgggc ggaaccgtgt
       61 cgaggtgtgc gcagaggaag atcgggctgc cgccgtccgt cgcctcgagc cgacagtaga
      121 cattgccgat gttcgagccg accttcgcgc ccgcgtcgtc ctcgtccacc gacaagccga
      181 gatcgcgcag atagccgatc acgacgtcgg cgactgcgcg ttcatcgccc ggcgggctcg
      241 gcagcgccgc cagctcggtg aaaaggtcga cgacctcgga ggccatcgct cgatcttatt
      301 tgtgggcgct agacggtaga ggctgcgccg actaccgacc gctctctcgc gcgcacgagt
      361 gcggcaccga cgaagtcgcg gaacagcggc gccggacgcg tcggccgcga cttgaactcc
      421 ggatgaaact ggctcgccac gaaccagggg tggtcgggca gctcgacgat ctcgactagt
      481 cggcccgcct ggaacgtgcc cgagacgagc aatccgccgt cgacgagcgc agggcggtag
      541 tggttgttga cctcgtaccg atgccggtga cgctcgtgca cgacgggctc gccgtaggcg
      601 tcgtacgcac gggttccctc caccacctcg accgcctgcg cgccgagcct cattgtgccg
      661 cccagatcct cgatctcctt ctgctcgggg agcaggtcga tcaccggatg cggcgtttcg
      721 ggatccatct cggtggagtt ggcgccgtcg aggccgagca catgccgcga gaattcggag
      781 acggcgacgt gcatgcccag gcagatcccg agatacggga tctcgcgctc gcgcgcgacg
      841 cggcaggcga ggatctttcc ttcccagccc cgggagccga aaccgccagg cacgagcact
      901 ccgtcgacct gttcgagcag cccggttgct tcctccagcg acatcccttc cgcatcgacc
      961 cagctcacac gcaccttcgc cccgtggtgc gcgcctgcgt gcttgagcgc ctcgtgcacg
     1021 gacagatagg cgtcctgcag cttcacgtac ttgccgacga gcgcgatctc gacgatctcc
     1081 tcgctctgct gcatgcgctc gttcaggtcg agccagtcgc ccaggtcggc ctcggtggcc
     1141 ggcagaccca gcttctcgcc gaccagacgg tcgagccctt cggccttcag ccgcgaagga
     1201 acgagataga catcggggac gtcggggctc gcgatcaccg cttccggctc gacgtcggcg
     1261 aacagcgcga tcttctcgcg gatgtccgcg gacagctcct cgtgcgaacg gcagacgacg
     1321 atgtcggggt ggattccgat gcggcgcagc tcgttgacgg agtgctgcgt cggcttcgtc
     1381 ttcagctcgc cggccgcttc caggaacggc acaagcgtca cgtgcaggta gaggacgttc
     1441 tccgcgccga cctcccggcg gaactggcgg atcgcctcga ggaacgggag cgactcgatg
     1501 tcgccgaccg tgccgccgat ctcgctgatc accacgtccg tcggagtcga gacggcgctg
     1561 gctcgaatcc gatgcttgat ctcgttcgtg atgtgcggga tcacctgcac ggtcgcgccc
     1621 agaaactcgc ccttgcgttc cttcctcagg accgagtccc agatcgcacc ggccgtgtgg
     1681 ctggccgcgc gcgtcaggtt ctcgtcgatg aagcgctcgt agtgaccgat gtcgaggtcg
     1741 gtctccgccc cgtcctcggt gacgaacacc tcaccgtgtt gaaacgggct catcgtgccg
     1801 ggatcgacgt tgagataggg atcgaacttt tgcgcctgca cacgcaaccc gcgtgccttc
     1861 aacagccggc cgagcgaagc cgcgacgatc cccttcccga gcgccgagac gacgccgccc
     1921 gtgacgaaga cgtacttcgt tggggaggct ggtgagctca tactctgccc agccgatcga
     1981 tccacgaaag aacgggggcc cgttcgatcc aggcaccgat ggactttcgc tctccgagga
     2041 tggtgaggct tgctagggcg ctgatcgcca cccactggcc tcttcccgtg aatcgcttca
     2101 cggaattcaa gcctagcagg gcgcccagtg cgttcgaccc cgcgtccccg agcatcgcca
     2161 tctcccgaag atcgtatgga agcagcagga cggcgacgcc gaccggagca tccagaggca
     2221 aggcggcagc aaggtacgcc ttgagtgcgc ggccggggcg cgtgtcgagc tggttgagta
     2281 cgttggcggc gagaccgacg agcagcgccc cggagacccg ccgggtcgca agcagcccga
     2341 ccgcagggat gccgaccagc ttgaggacgc cggtcgtccg gccggctcgc aggtgggcgc
     2401 cgaagcctcg ttcgggtccg ctccacaggt cgtcggcgag gccgagggcg cccaccgcgg
     2461 cggcggcggg accgcccgtt gcaccgacct tccagccgac gaggggcagg cgcaggcctc
     2521 ggaagttgat cccgagcggc ccgcaggcat agaccgtgtc gcgcagctgc cgcgcgcggt
     2581 gcacgaaccc ggagaggtcg cggcccgtgg atctgtggcc cagcggaagc tccacctcga
     2641 agacgcgcaa gcctgcgcgc gctgcatcga tcgtcatccg cacctcgcag ccgaacccgg
     2701 cggcaacggg gaagcacgcg gcccgcgcgg cggcagagag cgcgcgctgg cccgagaggg
     2761 gctcgcgcac gtcgagccct ccgagcacct cgatcagtcc acgggccacg cgcttcgcga
     2821 gaccgaagcc gcctccgact tgttccgcga acgcggcgat gttcagatcg ccgtcccccc
     2881 ggagaagggg ctcgagctcg cccgagagat cggcgtcgca cagcagcagc gctcccggcg
     2941 gcgcggcgcg ctcggcagcc gacaaggcct ggcccttgcc gcgacggggc aagcgcagga
     3001 ccagggcgcc cgcttcttcg gcgacttcgg cggtgcggtc gcgggagccg tcgtccgcca
     3061 cgatcacctc cccctccgga aacgctttgc gcaaccgcga gacggtcgac ccgatcgccg
     3121 cctcctcgtc tcgagccggg acgaggatgg tcagcggctc atccacccgc cgtggccgtc
     3181 agcggggaaa tgggcggcaa ctgggcctgg gcggtcttct tcgtaccgaa agcgcctgtt
     3241 gaagtcggat cggcgagcaa ggcgacgagc gcgagcttcc cgatccgcgt ctcgatgtcg
     3301 tcgaccgtcg aaaaaccggc ccgctggaac gctctgattg cgcttcccgg atcgttcgta
     3361 cgctcaacgc cgactacagg gacgccaccg tcggcaagcc cggcgtagag acctttgagg
     3421 aagagtgcgg ttggaccggt ctgagacgtc gcagtgcgaa cgatcacaac gccgtccgcc
     3481 ggaggtttcg acgggcccgc cttctcctcg acgatcaggc tctgcagcgc gatccacagc
     3541 ggcgtatccg tccccgcaac gaactcctgc cccaggtcgt gaccgaggtt ctgtagctga
     3601 tccggggcgg agtagcttgc gagcaacggc ttcgtcgcga gcttcctcgc caacgcagcc
     3661 gggtcgatcg ggacctggac gaacctcatt cgcaggggtg tgcctgcgcc tgcgtcggcg
     3721 agcgcctgcg ttatctcagt ccggcgagcc ttctcggagg agccgatgaa gaggacggcg
     3781 attttcttgc ccttgagcct gttcgtcata acgctcttgt acgtcccttc gacgaaggca
     3841 cgattgccgg cgccctcgtt catgagcgtc gcgacctgcg ttttgagtac gtcggcctgg
     3901 ctctccgcgc ggcgcaggtc ctcctcgagc cgcttccgct ccgtattgcc gagcccgtgt
     3961 gaggcgagcg caacgcccac cagaatgccg atgacgagcg caaagaagac cgcagcaagg
     4021 gacgcgacgt ggtagcggaa atcgaacata agggggcgag gagctcgtta gagctcagcg
     4081 gcagtctggc acaaggcgtg cagacgagtc agcgccctac gaggcagggc gcggctgaag
     4141 ccgtagagcc acggcgcctc gatgttgagc gtcgcgatcg tgcggtccgc cccacgctcg
     4201 tcgagtcgaa gttcggcgtc gatatggtcg ccggtcaacc tccaggcaaa tcgtttcggc
     4261 gtttctatgc ccgtcacgag cagcatgccg ctggacgtcg cacgtcgtag cagcgtgggc
     4321 cggtccgtgc cgctgatgcc ccagcgcgct ccttcggcga acccgcgccg gtcgggttgc
     4381 acgccgccga ttccgggcca ccagtccgca aaatgatgcg gctccgcgac gaacgaccag
     4441 acgtcctccc gcgctgcgag tagctcacgc gaagccgcgg ctcgagccat gcctgcagcc
     4501 tagtttcaac cgatcccgag caggtcgcgc agtcgctgtg cgaggtactc gagcagatgg
     4561 cgtagtgccg gagagaccag gatcgcgacg gcaatcgcgc ccagtcccgc gagcgtgaag
     4621 agcaccaatg gccagatgcc gacctgtctg ctcgccaggc gggagacgcc tttggcgtcg
     4681 atcaggatct cgcccacctt caagcgcgtc acgaacgtcg acgacatccc ggcgcggttg
     4741 cgctcgagga actcgatcag attgaaatgg gtgcctacgg caacgatcag ctccgagcct
     4801 ttctcctgtg cgagtagcag tgcgacgtcc tcgctgaccc cggtcgctcg cacgacctcg
     4861 tacttcacgc cgagctgagc tgcgcgttct tcccccggcg ctacgccatc gaggtagccg
     4921 tggacgagaa cctccgcgcc gctccgcaat gccttgtccg acacggagtc catgtccccg
     4981 acgatcacgt cgggcttgta gccggcctcg agtagggcgt cggagccgcc gtccacgccg
     5041 atcagcacgg gcttgaaagc gcggatgtag ggcttcacca tgcgcaggtc gcgcttgtag
     5101 ccggggccac gcgcgacaac aagcgcatgg cggtcacgaa agcccgtctt cagtggcgga
     5161 aactcgatcc cctccgccag cagctttcct tcctcccgca ggtagcgcat cgtgttctct
     5221 gcaaacgcct cgagcgcttc ggtgaccctg ccgcgttgtt cggcgagctt ccgttcgagg
     5281 tcacgctcct cgagcacctg tcccgtcgca aggcacgttc cattgcggta gagaccggcg
     5341 ccgcgcacgg tcagcaactc cccctcgttc acgtgctcga agagaccgtc agccgtcaca
     5401 tcgatcaggc ggatgcctcc gcgcacgagt gcgagcggtc ccggatttgg aaagcggccg
     5461 gattgcgacg gagcgacgtt caccacgacg cgcacgccgg actcgaggag tgcttccgcc
     5521 gagacgcgat cgagatccgt gtggtcgatg atcgcaatgt cgtcggcaga gagcctcttg
     5581 acgagattct tcgtgcggcg gtccaacttc gcatgcccgg tgagctcgac gaaactcatt
     5641 tttgtacgct cgagaggaac tctcgcccgc cgagcatgcg ctgcaactcg tcgcgccgct
     5701 cggcatcgtc gaggaggttg atgcgcgtgt gagtcgggtc acccgcgatc ttctcgaccc
     5761 ggaaatgccg atcggcgacg ctcgcgatct gcggcagatg cgtgatggtc acgacctgag
     5821 cgcggccggc gagggcgcgc aacgcctcgg cgaccgcgtg agcggtctcg ccgccgatcc
     5881 cggcgtcgat ctcgtcgaag accagcgtct cgccaccgcc gaccgcggcg atcgcgagcg
     5941 cgacgcgaga cagctccccg ccggacgcgg tctcggcgac cggaccgaag ggaaggccgg
     6001 cattcggccg gatcaggaag acggcctcat ccactcccgt cggcccggga tcg
//
LOCUS       DASVEU010000124         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1442726,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000124 DASVEU010000000
VERSION     DASVEU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442726"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VF225_05045"
     CDS             complement(<1..1364)
                     /locus_tag="VF225_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144656.1"
                     /translation="MGLLAVAAALASAGPADASLVHIDRTFGDLTVPRFRTGTLTIPR
                     NQASGRIRVIVGLQLAPLAAAYGRNLAGLGSTHRLDVSSMSSRLYLARIVTAQRVAAA
                     RLLRAIPAARIGRRYQVVLDGFVVTLPVTKLPRLARLGFVRKVYPSVRYHLATDTSPS
                     VIGATELQTLTGVKGDGIKIGIVDDGVDSTNPFLNPAGYSYPAGFPKGGRKWTTPKVI
                     VARAFPGPNSGRLGRLPIDREASFHATHVAGIAAGDAGTCSPGGADHPPTCGLSGVAP
                     RAWIGNYRVFNVPTPIGHIANTPEIAAAFEFAVRDGMDVINFSGGGAETEPANDAMID
                     VIRNVAAAGVVPVISAGNDRDQFGMGTVGSPGSAPEAISVAAVSNTHVFAPTLSARTA
                     GAPDNLRSIAIVGAGGGRFPDTFRLSGHRLVDVGALTGTDGKSVDRRLCGPDDDTNNP
                     DRSQL"
     gene            1416..2195
                     /locus_tag="VF225_05050"
     CDS             1416..2195
                     /locus_tag="VF225_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX7144657.1"
                     /translation="MSVTYEGKRGLVLGCANKRSIAWAIAKRLADAGAELAFTYQGAR
                     IEKGVRELAESISSPLVTECDVRSDEDVARVFREVGDMFDGGLDLLVHSVAFAAAEDL
                     EGRFTDTPRDRFWMALDVSAYSLVACARAAEPLLEARGGGSILTMTYLGGERAVPHYN
                     VMGVAKATLDASVRYLAWDLGQKNIRVNAISAGPVRTLSARSIAGFPTMEAIVEERSP
                     LHRHVNADDVAAAAAYLLSDDARNVTGTTLYVDSGYHAMGM"
     gene            complement(2182..>3297)
                     /locus_tag="VF225_05055"
     CDS             complement(2182..>3297)
                     /locus_tag="VF225_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144658.1"
                     /translation="FYSAHVGDFVSRHVDHARSEQFVFLGWATPLVALAGLVLLIRAR
                     RYALSAILGFGALVPIVLALGTRTPVYSALWHALPPFRFPRVPERLLPIACLSIAALF
                     AYAVAQSRRRIVVVLAIALLFVDLHARIYGKSAPGDPAGAVPSAAGRLLELPVFDPGV
                     HYGSAYLWYDTSAQRQRPGGYSTTAPKPAKTVADRLQRLNCGDWSGDMVGELERLGVG
                     SIALHRGLYVHNPAVPSTGWFASRGLLAHGWQVQRTAGPVWLFERAGSGLAPKRIEPA
                     RSGPVFCQGWFAETAGVGRYMSETHAPFWIYGSGRLKLDFAPSSLRPRVTIDSRSGSE
                     LRSEGWHLVTVDVDRLLKVAGQQHRVGLRLVRVTTSP"
BASE COUNT          556 a         1070 c         1184 g          487 t
ORIGIN      
        1 agttgcgatc tatcgggatt gttcgtgtcg tcgtcaggac cgcagagacg gcggtcgaca
       61 ctcttcccgt ccgttcctgt gagcgcgccg acgtcgacca gtcggtgacc gctgaggcgg
      121 aacgtgtcgg gaaagcggcc gccgccggcg ccgacgatcg cgatgctccg caggttgtcg
      181 ggcgctcccg ccgtgcgcgc cgagagcgtg ggggcaaaga cgtgcgtgtt cgacaccgcc
      241 gcgacgctga tcgcctcggg ggccgatccc ggcgagccga ccgtgcccat gccgaactgg
      301 tcgcgatcgt tacccgccga gatgaccggc acgacgccgg cagccgccac gttccgaatg
      361 acgtcgatca tcgcgtcgtt cgcgggctcg gtctccgcgc cgccgccgga aaagttgatc
      421 acgtccatac cgtcgcgcac ggcgaactcg aatgccgcgg cgatctcggg cgtgttggcg
      481 atgtgtccga tcggcgtcgg gacgttgaag acccggtagt tgccgatcca cgcgcgcggg
      541 gcgacgccgg agagcccgca cgtcggcggg tggtcagcgc ctcccggaga gcacgttccc
      601 gcgtcaccag cggcaatgcc cgcgacatgg gtggcgtgaa acgaagcttc gcggtcgatc
      661 ggaaggcggc ccagccggcc cgaattcggt cccggaaacg ctcgcgccac gatgaccttc
      721 ggcgtcgtcc acttgcgccc gcccttgggg aagcctgccg gataggagta accggctgga
      781 ttcaggaacg gattcgtgct gtcgacgccg tcgtcgacga tgccgatctt gatcccgtcg
      841 cccttcacac cggtgagcgt ctgcagttcg gtggctccga tcacggacgg gctcgtgtcg
      901 gtggcgaggt ggtagcggac actggggtag accttccgca cgaacccgag gcgggcgagg
      961 cggggaagct tcgtcaccgg gagcgtgacg acgaatccgt cgagcaccac ctgatagcgg
     1021 cggccgattc gcgccgccgg gatcgcccgc agaagccggg ccgcagcaac gcgctgcgcc
     1081 gtgacgattc gggcgagata gaggcgcgag ctcatgctcg agacgtcgag cctgtgcgtc
     1141 gaaccgagcc cggcgaggtt gcggccgtat gcggccgcga gcggggccag ctgcagcccc
     1201 acgatcaccc gaatgcggcc ggaggcctgg tttcggggga tcgtgagcgt tcctgtgcgg
     1261 aagcgcggga ccgtcaggtc cccgaaggtg cgatcgatgt gaacgaggga ggcgtcggcc
     1321 gggcctgccg acgcgagcgc cgccgcaact gcaaggagac ccactaaacg gcgcaagggg
     1381 accgcaatgt agccgaatgg atttatgctc gcgcaatgag cgtgacctac gaaggcaagc
     1441 gtggtttggt tcttgggtgt gcgaacaaaa ggtcgatcgc ctgggcgatt gccaagcggc
     1501 tcgcggacgc cggggccgag ctcgcgttca cgtaccaggg agcacgcatc gagaagggcg
     1561 tccgcgagtt ggcggagtcg atctcgagcc cactcgtgac cgaatgcgac gtgcgctccg
     1621 acgaagacgt cgcgcgtgtc tttcgcgaag tcggcgacat gttcgacggc gggctcgacc
     1681 tgctcgtgca ctcggttgcc ttcgcggcgg cggaggatct cgaaggccgc ttcaccgaca
     1741 cgccgcgtga tcgcttctgg atggcgctcg atgtgagcgc ttactcgctg gtcgcgtgtg
     1801 cgagggctgc ggagccgttg ctggaggcac gtggcggcgg ctcgatcctc acgatgacgt
     1861 atctcggagg cgagcgcgcc gttccgcact acaacgtcat gggcgttgcg aaggcgacgc
     1921 tcgacgcatc ggtgcgctat ctcgcctggg accttggaca gaagaacatc cgcgtcaacg
     1981 cgatttccgc cgggcccgtg cgcacgctct ccgcgcgctc gatcgccggc ttcccgacga
     2041 tggaggcgat cgtggaggag cgttcgccgc tacaccggca cgtcaacgcc gacgacgtcg
     2101 ccgccgccgc cgcgtatctg ctttccgacg acgcgaggaa cgtgaccgga acgacgctct
     2161 acgtggactc cgggtaccac gctatgggga tgtagtaacg cggaccaacc tcagcccgac
     2221 gcggtgctgc tggcccgcca ccttgagcaa ccggtcgacg tcgacggtga cgaggtgcca
     2281 cccttcagac cgcagctcgg aaccggagcg actgtcgatc gtcacgcgtg gcctgagcga
     2341 agaaggcgcg aagtcgagct tcagcctgcc gctgccgtag atccagaacg gcgcgtgggt
     2401 ctcgctcatg taccgcccca ccccggcggt ctcggcgaac cagccctggc agaagacggg
     2461 gccggagcgc gcaggctcga tgcgcttagg tgcaaggccg ctgccggcgc gttcgaagag
     2521 ccacacaggc ccggcggtgc gctgcacctg ccaaccgtgc gccagcaggc cgcgcgaggc
     2581 gaaccagccc gtgctcggta ctgccgggtt gtgcacgtag agcccgcggt gcagcgcgat
     2641 cgagccgacg ccgaggcgct cgagctcacc caccatgtcg ccgctccagt cgccgcagtt
     2701 gagccgctgg agacggtcgg cgaccgtctt cgccggcttc ggggccgtcg tcgagtagcc
     2761 gccgggccgt tgacgttgcg ctgaggtgtc gtaccagagg taggcgctgc cgtaatggac
     2821 gccgggatcg aagacgggca gctcgagcag acgtccggcc gcagacggca ccgctccagc
     2881 tggatcgccc ggcgcggact tgccgtagat gcgcgcgtgc aggtcgacga agagcaaagc
     2941 gatagcaagc acgacgacaa tcctgcgcct gctctgcgcc acggcgtacg cgaacaacgc
     3001 ggcgatggac aggcaggcga tcggcaggag tcgttccggg acgcgcggga agcggaacgg
     3061 cggcagcgcg tgccagagcg ctgagtagac cggcgtgcgc gttccgagcg cgagcacgat
     3121 cggtacgagc gcaccgaagc cgagaatggc tgagagcgcg tagcggcggg cgcggatgag
     3181 cagcaccagg ccggccagag cgacgagcgg cgtcgcccac ccgaggaaga cgaactgctc
     3241 gctgcgcgcg tgatcgacat gtcgcgagac gaagtcgccg acgtgcgccg aatagaa
//
LOCUS       DASVEU010000125         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_84127,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000125 DASVEU010000000
VERSION     DASVEU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84127"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF225_05060"
     CDS             complement(<1..523)
                     /locus_tag="VF225_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX7144659.1"
                     /translation="MSEHKIGTREEWQAARGELAKLEAQQAERNEEITSKRRDLPWVP
                     VEKEYEFDTEDGKKTLAELFDGRSQLLAYNIMFGPDYEIGACPGCSNLGDELDATRVH
                     LNHRDVTLICFSRAPIDRLTAYKERMGWQFPYVSTYATEFPFDFGLALTPEQAQEIPE
                     VKQMIDSPPGWLAD"
     gene            complement(609..1814)
                     /locus_tag="VF225_05065"
     CDS             complement(609..1814)
                     /locus_tag="VF225_05065"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX7144660.1"
                     /translation="MKVTAEAARRFLVARHFLAPARSLAGGLDGVLEVFRKFGSIQFD
                     PIAVAGRNHDLVLHARVAGYEPAWCDVLYERREIFESTNKALSFVPTSEFPWFRHVMG
                     RKGPRFHAAALAENTAVAERVLERIRAEGPLSSLDFERETGPTKDWFGMPENAVRAVL
                     EAYTVTGAIGLARRDGNRRYYDLLERLLPAPLLGQEVPEREQLLHKLLSRYRAHGLLG
                     PSGAGGTFARIAEPNARNELRKELVELGALVSVEVEGVRGKRLVLAEELALLQAPPEP
                     TRSVAFIAPFDSLLWDTALLSSLFAFDYVWEGFFPSAKRRWGYYVLPIVFGDRFVGRI
                     EPRIDRDRARVEVLNVWWEDGFAPRRADGFVDAMRDALRAYLRFAGAASLDWAPHLTT
                     EKRLFLTRP"
BASE COUNT          355 a          697 c          711 g          328 t
ORIGIN      
        1 agtcggcgag ccacccgggc gggctgtcga tcatctgctt gacctcgggg atctcctgcg
       61 cctgctccgg cgtgagggcg agcccgaagt cgaacggaaa ctccgtcgca taggtcgaga
      121 cgtatgggaa ctgccaaccc atccgctcct tgtaggcggt cagccggtcg atcggcgcgc
      181 gcgagaaaca gatcagcgtc acgtcccggt ggttcagatg gacgcgggtg gcgtccagct
      241 catcgcccag gttcgagcac cccgggcagg cgccgatctc gtaatccggc ccgaacatga
      301 tgttgtaggc gagcagctgc gagcgcccgt cgaagagctc ggcgagggtc ttcttgccgt
      361 cctcggtgtc gaactcatac tccttctcga ccgggaccca ggggagatcg cgacgcttgc
      421 tcgtgatctc ctcgtttcgc tccgcctgct gcgcctcaag cttggcgagc tcgccccggg
      481 ccgcctgcca ttcctcgcgt gttccaatct tgtgttcaga catctcggct ccttccgtcg
      541 agcgtggatc ggacgttaca gggatcgtta aatgagactc gcggcgagcc ggaaactcat
      601 cgcaaccgtc acgggcgggt gaggaagagc cgcttctccg ttgtgagatg gggtgcccag
      661 tcgagcgagg cagcgccggc gaagcgcagg taggcgcgga gcgcgtcgcg catcgcgtcg
      721 acgaatccgt cggcgcggcg cggcgcgaag ccgtcctccc accagacgtt gagcacctcc
      781 acgcgggctc ggtcccggtc gatccgcggt tcgatgcggc cgacgaagcg gtccccgaag
      841 acgatcggga gcacgtaata gccccaacgg cgcttggccg acgggaaaaa gccctcccac
      901 acgtagtcga aggcgaacag gctcgagagg agggcggtgt cccacagcag cgagtcgaac
      961 ggggcgatga acgcgacgga gcgggtcggc tccgggggcg cctgcagcag cgcgagctcc
     1021 tcggcgagga ccaagcgctt gccacgcacg ccttcgacct cgacggacac gagcgcgccg
     1081 agctcgacca gctccttgcg gagttcgttg cgcgcattcg gctcggcgat acgggcgaat
     1141 gtgccgccgg cgccgctggg gccaagcagg ccgtgggcgc ggtaccggga caggagcttg
     1201 tgcagcagct gctctcgctc gggcacttcc tgcccgagca acggggccgg gagcagccgt
     1261 tcgaggaggt cgtagtaccg gcgattgccg tcgcgccgtg cgaggccgat cgcgcccgtg
     1321 acggtgtatg cctcgagtac ggcgcgcacg gcgttctccg gcatcccgaa ccagtccttc
     1381 gtcggaccag tctctcgctc gaagtcgagt gaggacagtg gtccttccgc gcggatccgt
     1441 tcgagcacgc gctcggctac cgcggtgttc tcggcgaggg ccgcggcgtg gaaccggggc
     1501 cccttgcggc ccatgacatg acggaaccag gggaactcgc tagtcgggac aaacgacagc
     1561 gctttgttgg tcgactcgaa gatctcacgg cgttcgtaaa gcacgtcgca ccaggcgggc
     1621 tcgtagccgg cgacgcgcgc gtgcagcacg aggtcgtggt tccggccggc gaccgcgatc
     1681 gggtcgaact ggatcgaccc gaacttccgg aagacctcga gcaccccgtc gaggcctccc
     1741 gcgagagacc gcgcaggcgc gaggaagtgc cgcgcaacga gaaagcgacg cgccgcctca
     1801 gcggtgacct tcacccgcag gacactaacc gcgtgagcgt tgaccgactc gcgacgtatg
     1861 cgccgccaca ggcgaccatc acccacagac cttcgcgtag ttgcgaccgg ggacgaagcg
     1921 ccccggggga cattcccgaa cactcgccca cacaactctt tctaagagac cgcggttttg
     1981 tcgttctcgc gcacgcgaga gcggctgact ctcaggcttc gcgctcgatg ccgtccggca
     2041 gctgcaccca gggatgccgg cgcgtctcgt agatcgaccg aatcggcacg g
//
LOCUS       DASVEU010000126         5310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_475250,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000126 DASVEU010000000
VERSION     DASVEU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475250"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VF225_05070"
     CDS             complement(<1..1912)
                     /locus_tag="VF225_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7144661.1"
                     /translation="MTLTRRPWLYFGPAALVAGGIAVALVLTSDHEEHPAVATALGLF
                     VSWSFIFAGLIGWTRRPKNRTGMLMVAVGFGVFVGSLSEANYSVPYTLGALFGSLFIA
                     IFIHLLLAYPSGELISRKARTLVSAGYVTAFVAPLLDSMFPERHTCKPHACPDNLVFV
                     SHNSAAHWAQTALWTAASVALFVAAFALLVGRWRRGTPALRRILRPVYLAGGLSVVLL
                     AIGFIVTPFSGFGGTLVSVALIVTFTAVPFLFLAGLLGTTMARGAGLGTIFSSVPERA
                     SPGEVQEGLRQALRDNTAQVAYWYGEGGHYVDVDGNRFELPPDTRSRVVTRLDYADDP
                     VAAIVHDAALLEEPELLEGIANAARIALERDKLLVEVRARAQRYFALLQAMPDLMFRI
                     SKDGAYLSYNAPNPHDLIVDEVVGKTLWDRLPRDLADRVLTAGRAALAGEPSVIEYAL
                     DFDDETRHYEGRFAASGDEEFLMIVREITQRKQQQQELEASRQRIVAAGDDARRKLER
                     NLHDGAQQRLVSISLSLRLAQGQLEKNPGAAQEVLEASREELARALEELRELARGIHP
                     AVLTDRGLEAALESLAARAPLPVEIEGPLVSLPAAVEAAAYYVVSEALANVTKYAQAS
                     SVKVTVEQVNGCA"
     gene            complement(1909..2259)
                     /locus_tag="VF225_05075"
     CDS             complement(1909..2259)
                     /locus_tag="VF225_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144662.1"
                     /translation="MTVLIVDDHPSFRASARTLLEAEGYEIVGEAENGAAALQAVKDL
                     HPDLVLLDVQLPDMDGFQVAERLCGLANPPAVVLTSSRDSADYGSCIKICGASGFVPK
                     ADLSGAAIAALLIA"
     gene            complement(2314..4722)
                     /locus_tag="VF225_05080"
     CDS             complement(2314..4722)
                     /locus_tag="VF225_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7144663.1"
                     /translation="MTNVLLVDDRPENLLALEAILEPLEQKLLYAHSGEEALRQLLQH
                     DVAVILLDVQMPVLDGFETATLIKQRERTKHIPIIFVTAISKDEEHVFRGYSAGAVDY
                     VFKPFSPTVLRSKVAVFIELHEKTEQLRQQADQLKEQELAELRRESEERYRFLAEAQP
                     DQIWTAMPNGELDYVNQRVLDYFDSSFSERVAAGWTDVTHPDDLSRTLQLWQEALDTG
                     TPYDNELRLRRAADDSYRWHLTRAVPMRNRRGEIVKWFGSNTDIDDRKSAEEAQRFLV
                     EAGETLGSSLDYRTTLAALAKMAVPRIADWARVDIVEDGKLRTLAVEHVDERKVELAL
                     ELSRRYPEDPDAAQGPPLILRTGESELIAEISEERLSELAVDDLHLGLVRELGFQSYM
                     GVPLVVRGRGSGVISFVAAESGRRYGPADLALAEELARRASTAIENAQLYREAEERAQ
                     AARVLETVADGVFLVDVEGIVRLWNRAAEAITKLTRENVVGRRAGEVLPAWDDLAARV
                     PVAGAPGPATTTTFPLELEGEERWLSISGVGFDEGTVYAFRDLTEERALEQIRQDLVS
                     TVSHELRTPLAAIYGSALTLAREDLELEEAMHRKLLEVIVEESTRLADIVNDLLLASQ
                     LDAQRLEVHVEPCDAVALAASVLDAARTHLPAGTRVSLDSGEEDVPRVAADEGQLRQV
                     MANLLDNAVKYSPAGGDVHVRLEAAGKYVRFTVADEGLGIPAAEQSRIFEKFYRLDPD
                     MTGGIGGTGLGLYISRELVRRVHGRIWVEPNGAQGSVFHVEIPAASEPEAGQNRRKKA
                     GAGV"
     gene            complement(4719..>5310)
                     /locus_tag="VF225_05085"
     CDS             complement(4719..>5310)
                     /locus_tag="VF225_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7144664.1"
                     /translation="YDLICIGASWGGLQAVGRVLSDLPPELELPIAIAQHRHPDAQEA
                     TLAELLSLRTDRPVLDVEDKMPIQPQHVYIAPQNYHLLVERNSFALSVDERIRFARPS
                     IDVLFETAAHAYGAGVIGIILTGANDDGAHGLAKIKQAGGVAVIQDPLGAARRTMPDA
                     AIAATVADAVLPLEEIGKFIYGLWVQPAVRERAETT"
BASE COUNT          839 a         1773 c         1821 g          877 t
ORIGIN      
        1 gcgcacaacc gttcacctgc tcgaccgtca ccttgaccga ggaagcctgg gcgtacttcg
       61 tcacgtttgc aagcgcctcc gagacgacgt agtaggccgc agcctcgacg gcagccggca
      121 ggctcaccag cggcccctcg atctccaccg ggaggggagc gcgagccgcg agcgactcga
      181 gcgccgcctc gagcccacgg tcggtgagca ccgccgggtg gattccccgg gccagctcgc
      241 gcagttcctc cagggcgcgt gccagctctt ctcgtgaagc ctcgagcacc tcttgcgcag
      301 cccccggatt cttctcgagc tggccctgcg ccagccgcag ggacaacgag atcgagacga
      361 gtcgttgttg cgcaccatcg tggaggttgc gctccagctt ccggcgcgca tcgtcgccgg
      421 ccgcgacgat gcgctggcgt gacgcttcca gctcttgctg ctgctgcttt cgctgcgtga
      481 tctcgcggac gatcatcagg aactcctcgt ccccgctggc ggcgaagcgt ccttcgtaat
      541 ggcgggtctc gtcgtcgaag tcgagggcat actcgatcac gctcggctct ccggccagcg
      601 cggcccgtcc cgccgtcagc acgcggtcgg cgaggtcgcg tggcaggcgg tcccagagcg
      661 tcttgccgac gacctcgtcc acgatgagat cgtgtggatt gggcgcgttg taggagagat
      721 acgccccgtc cttcgagatg cggaacatca ggtcgggcat cgcctgcagg agggcgaagt
      781 agcgctgggc gcgggcgcgg acctcaacca gcagcttgtc ccgctcgagc gcgatgcgcg
      841 cggcgttcgc gattccttcc agtagctcgg gctcttcgag cagcgctgcg tcgtgcacga
      901 tggcagcgac cgggtcgtcg gcgtagtcga gccgggtgac gacgcgactg cgtgtgtcgg
      961 gcggcaactc gaagcgattg ccgtcgacgt cgacgtagtg cccgccttcg ccgtaccagt
     1021 acgcaacctg cgctgtgttg tcgcgcagcg cctgtcgcag gccttcctgc acctcgcccg
     1081 ggctggcgcg ctcgggtacc gagctgaaga tcgtgcccag cccggcgcct cgcgccattg
     1141 tcgtaccgag cagacccgcg aggaacagga acggcaccgc ggtgaaggtc acgatcagcg
     1201 cgacgctcac gagggtgccg ccgaagccgg agaacggcgt cacgatgaag ccgattgcga
     1261 gcagtacgac gctcagcccg ccggccagat agacagggcg aaggatgcgc cgcagtgccg
     1321 gcgtgccgcg tcgccagcgg ccgacgagca gcgcaaaagc tgccacgaag agggccaccg
     1381 aggccgcggt ccacagggcc gtctgagccc agtgggcagc gctgttgtgc gagacgaaga
     1441 cgagattgtc cggacaggcg tgcggcttgc acgtgtgccg ttccgggaac atggagtcca
     1501 gcagcggtgc gacgaacgcc gtcacgtacc ccgcgctcac gagcgtccgt gccttccggc
     1561 tgatcagctc gccgctcgga tatgcgagca ggaggtggat gaagatggcg atgaacaggc
     1621 ttccaaagag cgcgccgagc gtgtacggaa ccgagtagtt cgcctcgctg agactgccga
     1681 cgaatacgcc gaagccgacg gcgaccatca gcatcccggt gcggttcttc ggacgccgcg
     1741 tccagccgat caggccggcg aagatgaacg accagctgac gaacagtccg agcgcggtcg
     1801 ccactgcggg gtgctcctcg tggtcactcg tgagaacgag tgcaactgcg atcccacccg
     1861 cgacgagcgc agccggcccg aagtagagcc agggccgccg agtgagggtc acgcgataag
     1921 aagggccgcg atcgcggctc cggacagatc agccttcggg acgaagccgc tggcgccgca
     1981 aatcttgatg cagctgccgt agtcggcact gtcacggctc gaggtcagca cgaccgccgg
     2041 cgggttcgcc agtccgcaca gtcgttcggc gacctggaag ccgtccatgt ccggaagttg
     2101 cacgtcgagc agaacgaggt cgggatgaag gtctttcacg gcctggaggg ccgcggcccc
     2161 gttctcggct tcgccgacga tctcgtagcc ctctgcttcg aggagcgtcc gggcgctggc
     2221 ccggaaactg gggtggtcgt cgacgatcag aactgtcatg cccacagtgt cgtccccgag
     2281 tccaccttgc tgaatacggt tacccacact cttttagacg cccgccccag cctttttgcg
     2341 ccggttttgc cccgcttcgg gctcggaagc ggccggaatc tccacgtgga agacggagcc
     2401 ctgcgcgccg ttcggctcga cccagatcct gccatgcacg cgccggacga gctcgcgaga
     2461 gatgtagagg cccaggccgg tgccgccgat ccccccggtc atgtcagggt cgagccggta
     2521 gaacttctcg aagatccggc tctgctccgc ggctgggatg ccgagcccct cgtcggcgac
     2581 ggtgaagcgc acgtacttgc cggctgcctc caggcggacg tggacgtccc cgcccgcagg
     2641 tgagtacttc acggcattgt cgaggaggtt ggccatgacc tggcgcagct ggccctcgtc
     2701 cgcggcgact cgcggaacgt cttcctcgcc ggaatccaga ctgacccggg ttcccgccgg
     2761 taggtgcgtg cgcgcggcgt cgaggacgct ggccgccagg gcgaccgcat cgcaaggctc
     2821 gacgtgcacc tcgagtcgtt gggcgtccag ctggctggcg agcagaaggt cgttcacgat
     2881 gtcggccagc cgcgtggact cttcgacgat gacctcgagg agcttgcggt gcatggcctc
     2941 ctcgagctcg agatcctcgc gcgcaagcgt cagggcggag ccgtagatcg cggccagcgg
     3001 cgtgcgcaac tcgtgcgaga cggtcgagac gagatcctgt cgaatctgct cgagcgcccg
     3061 ctcctcggtg aggtcgcgga acgcatacac cgttccttcg tcgaagccga ctcccgagat
     3121 cgacagccaa cgctcttcac cctcgagctc cagcgggaag gtcgtggtcg tcgccggccc
     3181 gggcgctccc gcaacgggga cgcgcgccgc gaggtcgtcc caggcgggga gcacctcgcc
     3241 ggcccgccgg cccaccacgt tttcccgcgt cagcttggtg atcgcctccg ccgcgcggtt
     3301 ccagaggcgt acgattccct cgacgtcgac gaggaacacg ccgtccgcga ccgtttcgag
     3361 cacacgcgcc gcctgcgcgc gctcttcggc ctcgcgatag agctgggcgt tctcgatcgc
     3421 ggtggatgcg cggcgagcga gctcttcggc cagagctagg tcggcgggcc cgtaacgccg
     3481 gccggattcc gcggcgacga acgagatcac gcccgacccg cgcccgcgca ccacgagcgg
     3541 cacgcccatg tagctctgga agcccagctc ccggaccagg ccgagatgga ggtcgtccac
     3601 cgcgagctcg gacagccttt cctccgagat ctctgcaatg agctcgctct cgccggtgcg
     3661 caggatgagt ggagggccct gcgcggcgtc gggatcctcg ggatagcgcc ggctgagctc
     3721 gagcgcgagc tcgaccttgc gttcgtcgac gtgttcgacg gcaagcgtgc gcagctttcc
     3781 gtcctcgacg atgtccacgc gggcccagtc ggcgatgcgt ggcacggcca tcttcgcgag
     3841 agcggccagg gtcgtgcggt agtcgaggga gctgccgaga gtctcgcccg cctcgacgag
     3901 gaatcgctgc gcttcctccg cgctcttgcg gtcgtcgatg tccgtgttcg agccgaacca
     3961 cttgacgatc tcaccccggc ggttgcgcat cgggaccgcg cgcgtcaggt gccagcggta
     4021 ggagtcgtcc gccgctcggc ggaggcggag ctcgttgtcg tacggagtcc cggtgtcgag
     4081 cgcctcctgc caaagctgga gcgtgcggga aagatcgtcg ggatgcgtga cgtcggtcca
     4141 gcccgcggcg acgcgttcgg agaacgagct gtcgaagtaa tcgagcacgc gctggttcac
     4201 atagtcgagc tcaccgttcg gcatcgccgt ccagatctgg tcgggttgcg cttccgccag
     4261 gaagcggtag cgctcctcgc tctcgcggcg gagctccgcg agctcctgct ccttcaactg
     4321 gtcggcctgc tgccggagct gttccgtctt ctcgtgcaac tcgatgaaga cggccacctt
     4381 ggagcggagc actgtcgggc tgaacggctt gaagacgtag tcgaccgcgc ctgccgagta
     4441 gccgcggaag acgtgctcct cgtccttgga gatcgcggtg acgaagatga tcgggatgtg
     4501 tttcgtgcgc tcgcgctgct tgatcaacgt cgccgtttcg aaaccgtcga gcaccggcat
     4561 ctggacgtcc agcaagatca ccgcgacatc gtgctgaagc aactggcgga gagcctcctc
     4621 gccggagtgg gcgtagagga gcttctgctc gagcggctcg aggatcgcct cgagcgcaag
     4681 caggttctcg ggccggtcgt cgacgagcag gacgttggtc atgtggtctc ggcccgttct
     4741 cggaccgcag gctgaaccca aagcccgtag atgaacttcc cgatctcttc cagaggcagg
     4801 accgcgtcgg cgacggtcgc cgcgatggcc gcgtcgggca tcgtccggcg ggccgcgccg
     4861 agcggatcct ggatcaccgc cacgccgccg gcttgcttga tcttcgccag gccgtgagct
     4921 ccgtcgtcgt tggcgccggt gaggatgatc ccgatcacgc ccgcgccgta ggcgtgggcg
     4981 gcggtctcga agagcacgtc gatcgaaggc cgcgcgaacc ggatgcgctc gtcgacggac
     5041 agcgcgaacg agttgcgctc gaccagcaag tggtagttct gcggcgcgat gtagacgtgc
     5101 tgcggctgga tcggcatctt gtcctcgacg tcgagcaccg gacggtctgt ccgcaagctc
     5161 agcagctccg cgagcgttgc ttcctgcgcg tcgggatggc ggtgttgcgc gatcgcaatc
     5221 gggagttcga gctcgggggg caagtcggag agcacgcgcc cgacggcttg caatccgccc
     5281 caggacgcgc cgatgcagat caggtcgtac
//
LOCUS       DASVEU010000127         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_281501,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000127 DASVEU010000000
VERSION     DASVEU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281501"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VF225_05090"
     CDS             <1..767
                     /locus_tag="VF225_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7144665.1"
                     /translation="VRVHRVSQGSGVFLQTDDELDSMARVAADARVEISLFARPNAAW
                     DTSAMARSPAGGAVAPASRGQEQVVQGIEDVKRAAAHGIRSVLIADLGLLSVFGAMRA
                     AGELPGDMQAKISVMLPAANPATARVLAELGASTLNLPTDLTLAQIAAIRTAVDVPLD
                     LYVEAPDNIGGFVRLHEIPELIRIAAPVYIKFGLRNAPDVYPAGTHLEATTVALSRER
                     VRRARLGLELLARSGYEPTISELGAAGLAVPVLAAA"
     gene            767..1771
                     /locus_tag="VF225_05095"
     CDS             767..1771
                     /locus_tag="VF225_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7144666.1"
                     /translation="MARPVTLFTGQWADLPVEELAAKAREWGFDGLELACWGDHFEVD
                     KALADPGYVDSRRDVLDRNGLQAYAIGAHLVGQCVCDPIDQRHRAILPPEVWGDGEPE
                     GVRRRAAERLKDTARAATLLGVEQVNGFTGSSTWHMLYSFPPNDFAEIERGYQDFAER
                     WGPIMDVFAAEGVRFGLEVHPTEIAYDFVTTRKTLDAIGRRPEFGINFDPSHFAHQFL
                     DSAAFVEEFADRIYHVHVKDSRKTLDGRKGILGSHLNFGEAERGWDFVSPGHGDVDFE
                     SLFRALNRIGYAGPLSIEWEDSGMDRDWGAPDALAFVRRTDFAPSAIAFDAAMQRSAQ
                     "
     gene            1792..2994
                     /locus_tag="VF225_05100"
     CDS             1792..2994
                     /locus_tag="VF225_05100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7144667.1"
                     /translation="MSQPANAPPLAGVRVVDLSRVLAGPYATMTLADLGADILKIEHP
                     AGGDETRTWGPPFAGGESAYFLSVNRGKRSVALDLKDPEGLELALELCARADVVIENF
                     RPGGAARLGLDYEAVRRRRPSVVYCTISGFGAREPRDRAGYDFTVQAESGLMAITGEP
                     DGEPMKVGVAIVDVLAGLNAATAILAALHRRDLTGEGDFVEVSLLDSAFAALVNVGAN
                     ALLTGEEPLRYGNAHPSIVPYQPFRGTDGWIAVAAANDGLYARLCTAIERPDLAADER
                     YATNELRVLNRETLIAELEAVFAGRSTDDWERLLLAAGVPAGKIRGVGEALRTGQART
                     RRMDHPTAGTVELVGPPFELESATLGAATPPPLLGQHTAEVLRELGLDDERLALLEER
                     GVIARTPA"
     gene            3007..>3745
                     /locus_tag="VF225_05105"
     CDS             3007..>3745
                     /locus_tag="VF225_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7144668.1"
                     /translation="MAQIVREHTLLLDRGYIRGEWVEADSGATFPVLNPATREELAQV
                     PRMGADETRRAIEAAHDAYPAWRATLAKERARILRRWADLMLERRDDLALLLTTEQGK
                     PLAESRVEIEYAASFLEWFGEEAKRVYGDTIPTYMQDRRVVVTKEPVGVTAGITPWNF
                     PAAMVTRKAAPAIAAGCTMVLKPAEQTPLSALAVAKLAEEAGLPAGVFNVVTGDAGDA
                     PTIGGEMTSNPIVRKLGFTGSTEVGKLL"
BASE COUNT          587 a         1255 c         1307 g          596 t
ORIGIN      
        1 acgtccgtgt gcaccgggtc tcgcagggaa gcggcgtctt cctgcagacg gacgacgagc
       61 tggactcgat ggcacgcgtg gcagccgacg cgcgcgtcga gatcagcctc ttcgctcgtc
      121 cgaatgccgc gtgggacacg tcggcgatgg cgcgctcgcc tgcaggtgga gccgtcgcac
      181 cggcctcgcg tggccaggag caggtcgtgc agggcatcga agacgtgaag cgtgctgccg
      241 cgcatgggat tcgcagcgtg ctgatcgcgg acctcgggct gctctcggtg ttcggcgcga
      301 tgcgcgccgc aggtgagctc cctggcgaca tgcaggccaa gatctcggtg atgctgccgg
      361 ccgcgaaccc agcgaccgca cgcgtgctcg ccgaactggg cgcaagcacg ctcaacctgc
      421 ccaccgacct gacactggcc cagatcgccg cgattcgtac cgccgtcgac gtgccgctcg
      481 atctctacgt cgaggcgccc gacaacatcg gcggtttcgt gcgtctgcac gagatcccag
      541 agctcatccg tatcgcggcg cccgtgtaca tcaagtttgg tcttcgcaat gcgcccgatg
      601 tctatcctgc cgggacccac ctcgaagcga cgacggtggc cctgtcacgc gagcgcgtcc
      661 ggcgcgcacg gctcggtctc gagctgctcg cccgctccgg ctacgagccg acgatctcgg
      721 agctcggcgc cgccggcctt gccgttccgg tgttggcggc ggcctgatgg cgcgaccggt
      781 cacgctcttc accggccagt gggccgatct cccggttgag gagctcgctg cgaaggcgcg
      841 ggagtggggc ttcgacggcc tcgagctcgc gtgctggggc gaccatttcg aagtcgacaa
      901 ggcgctcgcc gatccgggct acgtcgattc gcggcgtgac gtgctcgacc ggaacgggtt
      961 gcaggcctac gcgattggtg cgcatctcgt aggccaatgt gtctgcgatc cgatcgatca
     1021 gcggcatcgg gcgattctgc ccccggaagt gtggggcgac ggcgagcccg aaggcgtgcg
     1081 ccggcgtgca gcggagcggc tgaaggacac ggctcgtgcg gccacgctgc tcggcgtcga
     1141 gcaggtcaac ggcttcacag gctcaagcac ctggcacatg ctgtactcgt tcccaccgaa
     1201 cgacttcgcc gagatcgagc ggggctatca ggatttcgcc gaacgttggg ggccgatcat
     1261 ggacgtcttc gcagccgagg gcgtccggtt cgggctggaa gttcatccga ccgagatcgc
     1321 gtacgacttc gtcacgacac ggaagaccct cgacgcgatc ggacgcaggc cggagttcgg
     1381 gatcaacttc gacccgagcc atttcgctca ccagttcctc gattccgcgg ccttcgtcga
     1441 ggaattcgcc gaccggatct accacgtgca cgtcaaggac tcccgcaaga cgctggacgg
     1501 acgcaagggc atccttggta gccatctcaa cttcggtgag gcggagcgcg gctgggactt
     1561 cgtctctccc ggtcacggcg acgtcgactt cgagtcgctc ttccgggcgc tgaacaggat
     1621 cggctatgcc gggccgctct cgatcgagtg ggaggactcg ggcatggacc gcgactgggg
     1681 agcaccggac gcgctggcgt tcgtccgtcg cacggacttc gccccgtcgg cgatcgcctt
     1741 cgacgcggcg atgcagcgct cggcccaatg agcgcggcct agactcgaac cgtgtcgcag
     1801 ccggcaaatg cgccgccgct tgcaggcgtg cgggtcgtcg atctctcgcg tgtgctcgcc
     1861 ggcccgtacg cgacgatgac gcttgccgac ctgggagccg acattctcaa gatcgagcac
     1921 ccggccggtg gagacgagac tcgcacgtgg ggccctccgt tcgcgggcgg cgagtccgcg
     1981 tacttcctct ctgtcaaccg gggcaagcgc agcgtcgccc ttgatctgaa ggatccggag
     2041 ggtcttgagc tcgcgctcga gctgtgcgca cgagcggacg tcgtgatcga gaattttcgc
     2101 ccaggcggcg cggcgcggct ggggctcgac tacgaggctg tgcggaggcg tcggccgagc
     2161 gtcgtctact gcacgatcag cggcttcggt gcgcgcgagc cgcgcgaccg tgccggttac
     2221 gacttcaccg tgcaggccga gagcgggctg atggcgatca ccggtgagcc cgacggtgag
     2281 ccgatgaagg tcggcgtcgc gatcgtcgac gtgctggccg ggctcaacgc ggccacggcg
     2341 attctcgccg cgctccatcg gcgtgatctc accggggaag gtgatttcgt ggaggtctcg
     2401 ctgctcgaca gcgccttcgc cgcgctcgtc aacgtcggcg cgaacgccct gctcaccggc
     2461 gaagagccgc tgcgctacgg gaacgcgcac ccgagcatcg ttccctacca gccgttccgt
     2521 gggaccgacg ggtggatcgc ggtcgccgcg gccaacgacg gcctctacgc gagactctgt
     2581 accgcgatcg agcggcccga cctcgccgcg gacgagcgtt acgccacgaa cgagctgcgc
     2641 gttctgaatc gggagacgct gatcgccgag ctcgaagcag tgttcgcggg ccgctccacc
     2701 gacgactggg agcgtctgct gctcgccgcc ggcgtgcctg cgggaaagat ccgaggcgtc
     2761 ggcgaggcgt tgcgcaccgg gcaggccagg actcggcgta tggatcatcc gacggccggc
     2821 accgtcgaac tggtcgggcc tcccttcgag ctcgagagcg caaccctcgg agcagcgacg
     2881 ccgccgcccc tgctcgggca gcacacggcg gaggtgctca gagagctcgg cctcgatgac
     2941 gagcggctcg ccttgctcga ggagcgcggc gtgatcgcga gaacacctgc gtagaatcgc
     3001 gccggaatgg cgcagatcgt gagagagcac accctcttgc tcgaccgcgg ctacatccgg
     3061 ggagagtggg tcgaggccga ctcgggtgcg accttcccgg tcctcaaccc ggccacgcgc
     3121 gaagagctcg cgcaggtccc gcgcatgggc gcagacgaga cgcgacgggc gatcgaggcc
     3181 gcacacgatg cgtatccagc gtggcgcgcg accctcgcga aggagcgcgc gcggatcctg
     3241 cgccgctggg ccgacctcat gctcgagcgc cgtgacgacc tggcgctgct cttgaccacc
     3301 gaacagggca agccgctggc cgaatcgcgc gtcgagatcg agtacgccgc ctcgtttctc
     3361 gagtggttcg gcgaagaggc caagcgtgtc tatggggaca cgattcccac gtacatgcag
     3421 gaccgacgcg tcgtcgtgac caaggaaccg gtcggcgtca cggccgggat cacgccgtgg
     3481 aacttccccg ccgcgatggt cactcgcaag gcggccccgg caatcgcggc cggttgcacc
     3541 atggtgctca agcccgccga gcagacgccg ctgtccgcgc tggcggtcgc gaagctggcc
     3601 gaggaagccg gcttgcctgc aggcgtgttc aacgtcgtca cgggcgacgc gggcgatgcg
     3661 ccgacgatcg gaggcgagat gacatcgaat ccgatcgtgc gcaagctggg cttcacgggc
     3721 tcgaccgagg tcggaaagct cttga
//
LOCUS       DASVEU010000128        13938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1445685,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000128 DASVEU010000000
VERSION     DASVEU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445685"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VF225_05110"
     CDS             complement(<1..1109)
                     /locus_tag="VF225_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7144669.1"
                     /translation="MPGRAKIEIPRWIQLAGFPVLLLFLWVMAGAVRHVVFLFLVALL
                     IALLLSPVVRLVQRVRIPRGFAVALVYLVFAVTLIAAIGALGTVVVSETKTAAKRVDS
                     YFTNVNGRTGVVDADRDVNRLQHWLDTHRLGGINVENRGHKFVRDIRKHDIGKYTNDV
                     VNFLEGAAISIGKLLFSVIVVLVVSIYMLLDFPKLERRINRRFPPESGSEPLLLRMER
                     SLTSYVKGQSLVSLIIGTSAGVGLWLFGVLGWLPHGQKYALLFGAWVAITELIPYLGP
                     WLGSIPPFLYALVVHPVSAVWVALLFLAIHQVEGHVVVPNVMGSALRLHPLLVIFGLL
                     AGGEIYGLPGALIALPLLAAARAMWEFFHERLGLEP"
     gene            1371..2084
                     /locus_tag="VF225_05115"
     CDS             1371..2084
                     /locus_tag="VF225_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144670.1"
                     /translation="MPHRARRLIVLTVVCGVLLGTLLITAFGQGATREPSQALPGPAS
                     RLLPVGPPTPLTVALHSGLRIQLPISQSRVTAIGYHSSGDGALPLSPLGRQGNESALS
                     RLAHKIFGGGGAGFTYYQLSGGETAALDIGALPGTDVYAPVDGTIVGISEYILNGRPY
                     GSLIEIQPSAAPSLVISLTHLSADPALTVGSMIAASSTKVGRVLDLSGVEKQALSKYT
                     QDAGNHVAIELRAAATLLP"
     gene            2164..2943
                     /locus_tag="VF225_05120"
     CDS             2164..2943
                     /locus_tag="VF225_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7144671.1"
                     /translation="MRILFIADAVGVPGRRAIEERLPSLREELGADLCILNGENLADG
                     VGITPKLADKMLASGVDAITLGNHVWRRGEIVPYLKESDRLIRPANLSKRAPGKGMTF
                     VTAADGTEVAVINLIGALFLGPPVGPFEVIDELVDEAQRRTPVIVVDMHAEATSEKVA
                     LAKWLDGKVTAVLGTHTHIQTNDARVQPGGTAAITDAGMTGPHDSVIGVQAELAIRAM
                     RTGMPVRFETASGDVRIEGVLFECDADGRATSCESVRVPVQ"
     gene            2940..4268
                     /locus_tag="VF225_05125"
     CDS             2940..4268
                     /locus_tag="VF225_05125"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7144672.1"
                     /translation="MTSAPIRYAENGDIHIAYQVVGEGPIDLVCVMGSITNLNVLWED
                     PEYRRLCERLASFSRVILFDKRGMGLSDRVRAGTLEERMDDVRAILDAVGSESAALLG
                     VSEGGPMSILFAATYPERTRALVLCGAEVKEETTDDWPWGEATRESFEEAVDIDRVVE
                     RWGKGGWIDHFAPSRAGDERLRQLAGRMQVESASPHDAVAFMRMAFEIDIRHVAPSVK
                     APTLILHRTGDKICHVENARWLARNVEGSRFVELPGADHLPWIDGDDILDEIQEFLTG
                     VREPAAPERMLATVLFTDIVGSTDRARELGDRRWRDLLERHNEIVRRDLERFRGVEID
                     TAGDGFFATFDGPARAIRCARSIVEDVRGVGLDVRSGLHTGECELSAGSARGIAVHTG
                     ARVASIAAPGEVLVSSTVKDLVAGSGIEFTDRGVHELKGIPGEWRLYAAT"
     gene            complement(4225..5475)
                     /locus_tag="VF225_05130"
     CDS             complement(4225..5475)
                     /locus_tag="VF225_05130"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX7144673.1"
                     /translation="MSSNTQASERSVVAAAFAAIALFVISWALLHVGFYKHKQLIDTP
                     VYQRYGNAIADGKVPYRDFELEYPPGALPIFALPGLADPGKDQYVTTGFRRTFETIMW
                     LCGAAALVAMAVVLRGLRRSTLNVWAALCFAAITPLLLGSVVLSRFDLWPAAIVAAVL
                     AALVSGRLRLGHALLGLGVTAKLYPAVLLPLGVAFVWKRAGRREALACLGLALAVIVA
                     VFAPFVILSPGGVWQSLHAQLSRPLQVESLGSALLLAGHHVFGLGVTGETSYGSQNLG
                     GGAADALAIASTVVQAALLLWIWASFARGPGSREALVRSSAATLCAFVAFGKVLSPQF
                     PIWLIPVVPLVRGRRGLLASALLAVALVLTQTWFPFRYFRLALNFEAGLSWLLLARDL
                     TLVALVAVLVVTLRRTTATRPESP"
     gene            complement(5499..7265)
                     /locus_tag="VF225_05135"
     CDS             complement(5499..7265)
                     /locus_tag="VF225_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144674.1"
                     /translation="MALLAAATFVPAASAAADPSLTKERATALFLENDKVADWLDRYP
                     RAGRVSDATYETDSSKCSAGTQGGCWSVTVTWKKNDKVDAGVIATGKVDDRTTRVTEA
                     WTGPQVAWKMARGYEGAFGGRKINSLRYWLPFCLVFLLGLADFRRPLSLRNLDLVALL
                     SFSVSLWFFNHGNIFTSVPLVYPPLVYLLARCVWIGVRGCPVRAARPLWKPAVLLAAA
                     VFIIGFRIGLNLADSNVIDVGYAGVIGAQRISSGVMPYDNFPKEDELKACGPADSAGE
                     IRDRIQSNGRCESANPLGDTYGPVAYEAYLPGYWIRGWSGKWDKLPAVHLTSIIFDVL
                     CALGLGLAGLRFGGRWLGAALVFAWAAFPFTQYASMSNTNDMIVPVFLIFGFWLASSP
                     WSRGVSVALSGLTKFAPLIVAPLWLTYPGPVRRPRPALAFAAGFVVATVVAFSILLLD
                     GHPIQAAHTFYERTLKTQITRESPFSLWDWAQYHARGIPDLHVLQRVLEGLLVLGAVA
                     VAFVPRTKTPLQLAALTGALLIGFELVLTHWSWLYIPWFFPFVVFALLSNGRGDSIEG
                     SAEGVSEDGRAGEAHTASIGVA"
     gene            complement(7286..7735)
                     /locus_tag="VF225_05140"
     CDS             complement(7286..7735)
                     /locus_tag="VF225_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7144675.1"
                     /translation="MDERDAAVAVGLRARVNQALRKRRNWEELAKFCVVGASGYVVNL
                     IVYVALLDGADLHYRLAASGSFLVAVTNNYLWNRLWTFRHDRGHFGHQGLRFFVVSVI
                     VYIGNLAILTLLVKLGVGKIVAQAIAIVTVTPANFIGNKLWSFRRLH"
     gene            complement(7756..8184)
                     /locus_tag="VF225_05145"
     CDS             complement(7756..8184)
                     /locus_tag="VF225_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144676.1"
                     /translation="MRERTGDELLALPVRLHGIQLGRPVDLLLDRDAQRIVGLDVLCG
                     DEVHRFLPLPTAAVGRGEIRILSPLVLLEQRELDFYRSRTLALSRLRDGVLERDGRQL
                     GRLQDLVVAEDGRVVAAIVDHKRIAIDAGLRFAPKRRSAA"
     gene            complement(8225..8869)
                     /locus_tag="VF225_05150"
     CDS             complement(8225..8869)
                     /locus_tag="VF225_05150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX7144677.1"
                     /translation="MPPDSAAEARSRMVDAQLRGRDVVDERVLDAMERVPRELFVPEK
                     LRDRAYDDAALPIGDGQTISQPYMVARICEALSLTGVERVLDVGTGSGYQAAVLAELA
                     AEVDTIERIPRLAERARANLEAAGYGRVSVHVGDGSRGLPDRAPFDAIAVAAAAPELP
                     RTLYEQLEPRGRLVVPVGRRGMQRLEVIVRSPEGPAVLRSVPCRFVPLLGAEGF"
     gene            complement(8859..10220)
                     /locus_tag="VF225_05155"
     CDS             complement(8859..10220)
                     /locus_tag="VF225_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX7144678.1"
                     /translation="MRSSGPLSLILIVIDGLTPSMFEAAVGDDRTPALSFLAEQGAYS
                     RAISTFPSLTPVCLSSIATGAHPDVHRIPHLVWYDRERARLVEYGSSFGAVRAAGMTR
                     SLLDTTINMNRLHLGTEAVTVYEALEDAGLTAAAINITCYRGRTPHLPTLPGLTFPAY
                     GPKRFFFYNLFESDPTGAPLAVFGRSHGSIDGYAAAVGRWLVTRDGFDFLVYYLPDYD
                     FASHAHGPDEAFEALTRTDAAIGALLEAAGGPDEFLERYAVVLCSDHGQTHVDRHVRL
                     EDSFGDLDDVLVTASNRAGMIYRLGGAAPDARTLAERLDEQESAETVLFLEGADAIAR
                     REGEELRFASGVGGWITTGDEGLLGYPGGLERAWAALNNSNAGEVLVSAAPGFEFADL
                     AGRHHSGGGSHGSLRRGDSEVPMLTIGIDAQPQSIVEIAPTVLRHFGVAPPAYARTLT
                     RAA"
     gene            complement(10363..10782)
                     /locus_tag="VF225_05160"
     CDS             complement(10363..10782)
                     /locus_tag="VF225_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144679.1"
                     /translation="MGSWYWIGVCAGLGVAVGVLLAGFLIGTRAALTAALVLAAGAGV
                     AVGFALGQWDEAVGGGVGGALGSIGAAQLVSGALRRGGSRFGTAIFIALAAVVLAAVA
                     WIPVAGYLEATLLPALALRLRGRAGERYAGLRSLAKD"
     gene            10916..11476
                     /locus_tag="VF225_05165"
     CDS             10916..11476
                     /locus_tag="VF225_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7144680.1"
                     /translation="MRHRGVIATLAVGVAALGIAAFALAGGSGARFNHLKATMSGYQE
                     VPSVSSTGTADFTADVSQDGSSIAWQLSYSGLEGAVQQSHIHFGQRSVNGGISVFLCT
                     NLGNGPAGTQACPQSGTISGTIDAADVSPAIPATAAARTQGIDTGEYDELVRAIGAGK
                     AYANLHTDKWPGGEIRAQLNEDDPHN"
     gene            complement(11517..12566)
                     /locus_tag="VF225_05170"
     CDS             complement(11517..12566)
                     /locus_tag="VF225_05170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7144681.1"
                     /translation="MAYEFKLPDLGEGLTEGEVARWLVSEGQEIGEDDPLVEIQTDKT
                     TVEIPSPAAGTVTSILVDEGKVVPVGTVLVVIGGKPDGEGPRAATKTTASERPTKGKA
                     TPLVRKIAQELGVDLDTVAGTGPQGRITEDDVRATAGPSEGRREPLRGVRRVIAEHMA
                     RAHREVPAVTWVEECDFSGLDLKQLVPTVLKACAETLQEFPELNARLERDEIVYLDRY
                     DLGVAVQTDEGLVVPVVRDCATRSIEDLGADVARLAESARAGSLKPEELRGSTFTVTS
                     AGKLGGLLQTPIVNYPEVAILSIGRIGPRPVVRDGEVVAAQIGYVSVTFDHRVVDGAR
                     AAEFGLAVIRRLEQR"
     gene            complement(12569..13483)
                     /locus_tag="VF225_05175"
     CDS             complement(12569..13483)
                     /locus_tag="VF225_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX7144682.1"
                     /translation="MTHEIPLERRTLHGHFSRDLAPVATIESGDTVAFSCLDSAWHVA
                     PGEKFEPRDEELDAGHALVGPIEVRGAKAGQTLAVRIDEVRVGSFGATLAGGWSTPLN
                     DRLDMSQGEKLTLHWSLDSEAGTGRDPQGREVELRPFPGVLGMPPPEPGVHPTAPPRR
                     WGGNIDCKELVAGTTLFLPIPVDGALFSAGDGHARQGDGEVSQTAIECPLDRLELTLT
                     VEDRTELKTPIAWTPDAWLTFGFDEDLDEAAAFAVDAMLDLMQRERDLERVDALALAS
                     VVVDLRVTQLVNGVKGVHAVLAHDAIRM"
     gene            complement(13480..13902)
                     /locus_tag="VF225_05180"
     CDS             complement(13480..13902)
                     /locus_tag="VF225_05180"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX7144683.1"
                     /translation="MSERQTNERLRLWLERSGAGFRLRDAATGELVRGEDPRIRVIKV
                     AGVSYRIEALQDEAFAPGRRLALVAEPDNEHDPHAIGVWDSARRVQAGYVPAEIAREL
                     EADEWQAVTLWEFFEEGSRGGLRVLLAPRDAWIGVPRT"
BASE COUNT         2454 a         4736 c         4727 g         2021 t
ORIGIN      
        1 ggctcgagcc ccaggcgttc gtggaagaac tcccacatcg cgcgggcggc agcaagcagc
       61 ggcagcgcga tcagcgcgcc cggcaagccg tagatctcgc cgccggcgag caggccgaag
      121 atgacgagca acggatgcag ccgcaacgcg ctccccatca cgttcggaac gaccacgtga
      181 ccttccacct gatggatcgc gaggaacaga agcgcgaccc agaccgcaga gacgggatgg
      241 acgacgagtg cgtagaggaa cggcgggatc gagccgagcc atggtccgag gtacggaatg
      301 agctcggtga ttgcgaccca ggctccgaag agcagcgcgt acttctgccc gtgcggtagc
      361 cagccgagca cgccgaagag ccacagtccc acgcctgcgc tcgtgccgat gatcagcgag
      421 acgagtgatt gtcccttcac gtagctcgtc agcgaccgct ccatgcggag cagcagcggc
      481 tccgagcccg actcgggtgg aaaccgcctg ttgatccgcc gttcgagctt ggggaagtcg
      541 agcagcatgt agatcgacac gaccaggacg acgatcacgc tgaagagcaa cttcccgatc
      601 gagatcgccg cgccctcgag aaaattgacc acatcgttcg tgtacttgcc gatgtcgtgc
      661 ttgcggatgt cgcgcacgaa cttgtggccg cgattctcga cgttgatgcc gcccaggcga
      721 tgcgtgtcga gccagtgctg cagacggttc acgtcgcggt cggcgtcgac gacgccggtt
      781 cgtccgttca cgttcgtaaa gtaggaatcc acccgtttcg cggccgtctt cgtctcactg
      841 acgaccacgg tcccgagagc cccaatggcg gcgatcaacg taaccgcgaa gacgaggtac
      901 accaacgcga ctgcgaagcc gcgagggatt cggacccttt gcaccagccg gacgaccggg
      961 ctcagcagca gcgcgatcag cagcgccacg aggaagagaa agacgacgtg acggaccgcg
     1021 cccgccatca cccagagaaa gagcaacagg accgggaacc cggccagctg gatccagcgc
     1081 gggatctcga tcttggccct gccgggcatg cgggtgatcg tacagccgac gtcctacggt
     1141 tccccctgca gcgaccccct gcttgggact gggactgggt cgaggagggc aacctcgccc
     1201 tcgaccgtca cgaaatgtgg gcagaaacgc aggtggatcc gaatgttcga ctgtcacaaa
     1261 gtcggccccc tcatcggttg aaggaggggt ccgggtccgt tacggtgccg cggatgccgg
     1321 ccccccctcg ccgagcctcc gcggcgcgtc agcgtccgcg gggacagccc atgccccacc
     1381 gggcgcgacg cctcatcgtc ctgaccgtcg tctgcggcgt cctgctgggg acgcttttga
     1441 tcacggcgtt cgggcaagga gcgacgcgcg agccctctca ggcgctgccg ggcccggcca
     1501 gccggctgct gccggtcgga ccgccgacgc cgctcaccgt ggctctacac agcggactgc
     1561 gcatccagtt gccgatctcc cagagccgcg tcacagcgat cggctatcac tcctccggcg
     1621 acggcgcgct gccgctcagc ccgctggggc ggcagggcaa cgaaagcgca ctctcgcggc
     1681 tcgcgcacaa gatcttcggc ggcgggggcg cgggcttcac ctattaccag ttgagcggag
     1741 gcgagaccgc ggcgctcgac atcggcgcgt tgcccggcac ggatgtctac gcgccggtcg
     1801 acggcacgat cgtcgggatc tcggagtaca ttctaaacgg ccgaccctac ggctcgctga
     1861 tcgagatcca accgtcggcc gcaccgtcac tcgtgatctc gctcacgcac ctgagcgctg
     1921 acccggcgct caccgtcggt tcgatgatcg ccgcgtcgag caccaaggtc ggccgcgtgc
     1981 tcgatctgtc cggggtcgag aagcaggcgc tctcgaagta cacgcaagac gcgggcaacc
     2041 acgtggcgat cgagctgcgc gccgccgcga ctctcctgcc ctaacccgcg caaagtgctt
     2101 cgcactttcc gcggtcatga gttagacctt cgcttcgctc ggtaaacccg cgagaatcct
     2161 tccgtgagaa ttctttttat tgccgatgcg gtcggcgtgc ccggccgtcg cgccatcgag
     2221 gagcgtctcc cttcattgcg cgaagagctg ggcgcggatc tctgcatcct gaacggcgag
     2281 aaccttgcag acggcgtcgg catcacgccc aagctggccg acaagatgct cgcctcgggc
     2341 gtcgacgcga tcacgctcgg caatcacgtt tggcggcgcg gcgagatcgt gccgtacctg
     2401 aaggagagcg accgcctgat cagacccgcg aacctgtcga agcgcgctcc gggcaaggga
     2461 atgacgttcg ttacggcggc cgacgggacc gaggtcgctg tgatcaacct gatcggcgcg
     2521 ctctttctcg ggcccccggt cggtccattc gaggtgatcg acgagctcgt cgacgaggcg
     2581 cagaggcgca caccggtgat cgtcgtcgac atgcacgccg aggcgacgag cgagaaggtg
     2641 gctctggcca agtggctcga cgggaaggtg accgcggtcc tgggaacgca tacgcacatc
     2701 cagacgaacg acgcccgcgt gcaaccgggc ggcacggcgg cgatcacgga cgcggggatg
     2761 accggcccgc acgattccgt gatcggcgtc caggccgagc tcgccatcag ggcgatgcgc
     2821 acgggcatgc ccgtgcgctt cgaaacggcc tccggcgacg tgcggatcga gggagtcctc
     2881 ttcgagtgcg acgccgacgg tcgcgccacg agttgcgagt ccgtgcgcgt accggtgcaa
     2941 tgacctcggc gcccatccgt tacgccgaaa acggcgacat ccacatcgcc taccaggtgg
     3001 tcggggaagg tccaatcgat ctcgtctgcg tgatgggctc gatcacgaat ctcaacgtgc
     3061 tctgggagga cccggagtac cggcggctct gcgaacggct cgcctcgttc tcccgggtca
     3121 tcctcttcga caagcgcggc atggggctgt ccgaccgggt gcgcgcgggc acgctcgagg
     3181 agcgaatgga cgatgtccga gccatcctcg acgcggttgg atccgagagc gcggcactgc
     3241 tgggcgtctc ggagggcggg ccgatgtcga tcctcttcgc cgcgacctat cccgaacgaa
     3301 cgcgggccct cgtgctctgc ggggcagagg tgaaggagga gacgacggac gattggccct
     3361 ggggcgaagc cacgcgcgag agcttcgagg aggctgtgga catcgaccgc gtcgtcgagc
     3421 gctggggcaa aggaggctgg atagatcact tcgcacccag ccgcgccggg gacgagcgcc
     3481 tccgccagct tgccggacgc atgcaggtcg agtcagcgag cccgcatgac gcggtggcgt
     3541 tcatgcgcat ggccttcgag atcgacatcc gtcacgtcgc gccttcagtc aaggcgccga
     3601 ccctgatctt gcaccggacg ggcgacaaga tctgccacgt cgagaacgca cgctggctgg
     3661 cacggaacgt cgagggctct cgcttcgtcg agctgccggg agccgaccat ctcccgtgga
     3721 tcgacggcga cgacatcctc gacgagatcc aggagttcct gaccggcgtg cgcgaacccg
     3781 cggcgccgga gcgaatgctg gcgacggtcc tgttcacgga catcgtcggc tcgaccgacc
     3841 gggcgcgcga actaggtgac cggcgctggc gcgatctgct cgagcgtcac aacgaaatcg
     3901 tccgtcgcga cctcgagcgc tttcgcggag tggagatcga cacggccggc gacgggttct
     3961 tcgccacctt cgacgggccg gcacgcgcga tccgttgcgc gcgctcgatc gtcgaggacg
     4021 tgcgcggcgt tggtctcgac gtacgctcgg gcctgcacac cggcgagtgc gagctgagcg
     4081 ccggctcagc ccgggggatc gccgtccaca cgggcgcacg cgtcgcctcg attgctgctc
     4141 ccggcgaggt gctcgtgtcg agcaccgtga aggacctcgt cgcgggatcc ggcatcgagt
     4201 tcaccgaccg cggcgtgcac gagctcaagg ggattccggg cgagtggcgg ttgtacgccg
     4261 caacgtgacc acgagcaccg cgacgagcgc gaccagcgtg aggtcgcgcg cgaggagcag
     4321 ccaggaaagc cctgcttcga agttgagcgc aagcctgaag tagcggaacg ggaaccacgt
     4381 ctgcgtgagg accagcgcaa cggcgaggag cgcgcttgcg aggagccctc gacggccacg
     4441 cacgagcggc acgaccggaa tcagccagat cgggaactgc ggcgagagga ccttgccgaa
     4501 cgcgacgaac gcgcagagcg tggccgcact cgaacgaacg agtgcctccc ggctccccgg
     4561 cccgcgcgcg aacgatgccc agatccaaag cagcagggcc gcctgcacga cggtcgacgc
     4621 gatcgccagc gcgtcggcgg cgccgccgcc gagattctgg gagccgtagc tcgtctctcc
     4681 cgtcacacca agcccgaaca cgtggtgacc ggccaggagt aacgccgagc ccaggctctc
     4741 cacctgcagc ggccggctca actgcgcgtg gaggctctgc cagacgccgc cgggcgacag
     4801 gatgacgaaa ggcgcgaaga ccgccacgat gacggccagc gcgagcccca gacaggcgag
     4861 cgcctcccgc cggccggcgc gcttccacac gaacgcgaca ccgagcggca acagcacggc
     4921 cggatagagc ttcgcggtga cgccgagccc gagcaacgcg tggccgaggc ggagtcgtcc
     4981 cgagacgagc gcggcgagga cggcggcgac aatcgccgcc ggccagaggt cgaagcggct
     5041 cagaacgacc gagcccaaca gcagcggcgt gatcgccgca aagcagagcg cggcccagac
     5101 gttcagtgtg ctgcgccgca gtccacgcag gacgaccgcc atcgcgacga gcgccgctgc
     5161 gccgcacaac cacatgatcg tctcgaaggt gcgacgaaaa ccggtcgtga cgtactggtc
     5221 tttgccgggg tcggcaagcc cgggcagggc gaagatgggc aacgcaccgg gcggatactc
     5281 gagctcgaag tcgcggtagg ggacctttcc gtccgcaatc gcattgccat agcgctggta
     5341 gaccggtgtg tcgatcagct gcttgtgctt gtagaagccg acgtgcagca gcgcccacga
     5401 aatgacgaag agcgcgatcg ccgcaaacgc cgcggcgacc accgagcgtt cagacgcttg
     5461 cgtgttgctg ctcacggcca ttgaagccca ggcgtcgact aggcgacgcc gatagaggcc
     5521 gtgtgggctt cgcccgcgcg gccgtcttca ctaacgccct cggctgatcc ttcaatcgaa
     5581 tcaccgcgcc cattcgacag cagagcgaac acgacgaagg ggaagaacca cgggatgtac
     5641 agccaggacc agtgggtgag cacgagctcg aagccgatca ggagcgcccc cgtcaacgca
     5701 gccagctgca gcggtgtctt ggtgcggggc acgaacgcca ccgccacggc tccgagcacc
     5761 agcagcccct cgagcacccg ctgcaggacg tggagatccg gaattccgcg cgcgtggtac
     5821 tgggcccagt cccagagcga gaacggcgac tcgcgcgtga tctgcgtctt gagcgtgcgt
     5881 tcgtagaacg tgtgcgctgc ctgaatcggg tgaccgtcga gcaacaggat ggagaacgcg
     5941 acaaccgtgg cgacgacgaa tcccgcggcg aacgcaagcg ccgggcgagg tcgccggacc
     6001 gggcccggat acgtcagcca gagcggggcg acgatgagcg gcgcaaactt cgtcaagccg
     6061 gagagagcga cggagacccc gcgcgaccag ggagagctcg ccagccagaa cccgaagatg
     6121 agaaagaccg gcacgatcat gtcgttggtg ttcgacatcg acgcgtattg cgtgaacgga
     6181 aaggcggccc acgcaaacac gagcgcggcg ccgagccagc gtccgccgaa gcgtaagccg
     6241 gcgaggccga gcccgagcgc gcagagcacg tcgaagatga tcgaagtgag gtgcacggcc
     6301 ggaagcttgt cccacttccc ggaccagccg cggatccagt agccgggcag atacgcctcg
     6361 taggcaaccg gaccgtacgt gtcccccagc gggttcgcag attcgcagcg gccgttggac
     6421 tggatgcgat cgcggatctc tcccgccgag tcggcagggc cgcatgcctt cagttcgtct
     6481 tccttcggaa aattgtcgta cggcatcaca ccgctcgaga tgcgctgcgc gccgatcacg
     6541 ccggcgtagc cgacgtcgat cacgttggag tccgcaaggt tgaggccgat ccggaagccg
     6601 atgatgaaga ccgcggccgc gagcagcacc gccggcttcc agagcgggcg cgccgcgcga
     6661 actggacacc cgcgcacgcc gatccagacg caacgtgcga gcaggtacac gagcggcgga
     6721 tagacgagcg gcacgctcgt gaagatgttc ccgtggttga agaaccagag ggagacggag
     6781 aaggaaagca gcgcgaccag gtcgaggttc cggagtgaca gcggccggcg aaagtccgcg
     6841 agcccgagca ggaacacgag gcagaaggga agccagtagc ggaggctgtt gatcttcctg
     6901 ccgccgaatg cgccctcgta gccgcgcgcc atcttccatg cgacctgcgg gccggtccag
     6961 gcttccgtga cgcgagtcgt gcggtcgtcg accttgccgg tcgcgatcac gccggcgtcg
     7021 accttgtcgt tcttcttcca cgtgacggtc acgctccagc acccgccttg cgttccggcg
     7081 gagcacttcg aggagtcggt ctcgtaggtc gcgtcgctga cacgccccgc tcgcggatag
     7141 cggtcgagcc agtccgcaac cttgtcgttc tcgaggaaga gcgccgtggc gcgttccttc
     7201 gtcaggctcg ggtcggcagc cgccgaggca gccgggacaa aggtcgctgc cgcgagcaag
     7261 gccacggccg cgaacaggat cagcctcagt gcagtctccg gaacgaccag agcttgttgc
     7321 cgatgaagtt cgccggcgtg acggtgacga tcgcgatcgc ctgcgccacg atctttccga
     7381 cgcccagctt gaccagcagc gtgaggatgg cgaggttgcc gatgtagacg atcaccgaca
     7441 ccacgaagaa ccgcagaccc tggtgtccaa aatggccgcg atcgtgacga aacgtccaga
     7501 gccggttcca aagatagttg ttcgtcaccg cgacgaggaa cgaaccgctc gccgcgaggc
     7561 ggtaatgcag atcggcgccg tcgagcagag caacgtagac gatcaggttg acgacgtaac
     7621 ccgatgcccc cacgacgcag aatttcgcga gctcctccca gttgcgtcgc ttgcgcaggg
     7681 cttgattgac gcgggcacgc aaacccacgg cgacggctgc gtcccgctcg tccatgcggc
     7741 tggagaaact agctgttaag ccgcggagcg gcgtttggga gcgaaccgca gcccggcgtc
     7801 gatggctatc cgcttgtggt cgacgatcgc cgcgaccacc cgcccgtcct ccgccacgac
     7861 gaggtcctgg agccgcccta gctgtcgccc gtcccgctcg agcacaccgt cacgcagccg
     7921 gctcagcgcc agagtccgcg agcggtagaa gtccagctcg cgctgctcga ggaggacgag
     7981 gggcgacagg atccggatct ccccgcggcc gacggctgcc gtcggtaggg gcaggaagcg
     8041 gtgcacctcg tcgccgcaaa ggacgtcgag ccccacgatc cgctgggcat cgcgatcgag
     8101 aagcagatcg acagggcgcc ccagttggat cccgtgcagt cggacgggca gggccagcag
     8161 ctcgtctccc gttctctccc tcatccagcc gccatcgtgc ccggaccgac cggacgcaaa
     8221 cgcttcagaa cccctcggcg cccaggagag ggacgaagcg gcagggcacc gagcggagga
     8281 cggcggggcc ttccgggctg cggacgatca cctcgaggcg ttgcatcccg cgccgcccaa
     8341 ccggcaccac cagacgtcca cgcggctcga gctgctcgta gagcgtccgt ggcagctccg
     8401 gcgccgctgc tgccaccgcg atcgcgtcga agggcgcacg gtcgggaagg ccgcgcgagc
     8461 cgtcgccgac gtgaacgctg acccggccgt agcccgctgc ttcgaggttc gcgcgcgcac
     8521 gctcggcgag cctcgggatg cgctcgatcg tgtcgacctc cgcggcgagc tcggccagca
     8581 ccgctgcctg gtagcccgag cccgtgccga cgtcgagaac gcgctccact cccgtcagcg
     8641 agagcgcctc gcagatccgc gcgaccatgt acggctggga aatcgtctga ccgtcaccga
     8701 tcggcaaggc agcgtcgtcg taggcgcgat cgcgcagctt ctcgggcacg aagagctcac
     8761 gcgggactcg ctccatcgcg tcaagcacgc gctcgtcgac aacgtccctg ccgcgcagct
     8821 gcgcgtcgac catccggctc ctggcctcgg cggcggaatc aggcggcacg cgtgagcgta
     8881 cgggcgtagg cgggaggcgc cacgccgaaa tgccgaagaa ccgtcggcgc gatctccacg
     8941 atggactgcg gttgcgcgtc gatcccgatc gtcagcatcg gcacctccga gtcgccgcgg
     9001 cgcagggaac cgtgactgcc cccgccggag tgatggcgtc ccgcgagatc ggcgaactcg
     9061 aaccccgggg cagcagagac gagcacctcg ccggcgttcg aattgttcag cgcggcccac
     9121 gctcgctcca ggccgcctgg atacccgagc aggccctcgt cccccgtcgt gatccatcca
     9181 cccacgcccg aggcgaagcg cagctcctct ccttcgcgcc gcgcgatcgc gtccgctccc
     9241 tcgaggaaca agaccgtctc tgcggactcc tgctcgtcga gtcgctccgc aagcgtccgt
     9301 gcatccggcg ccgccccgcc gagcctgtag atcatcccgg cccggttcga cgcggtgacg
     9361 agcacgtcgt cgaggtctcc gaacgagtcc tccaggcgaa catgccggtc gacgtgagtc
     9421 tgcccgtggt cggagcagag aaccaccgcg tagcgctcga ggaactcgtc cggtccaccg
     9481 gccgcttcga ggagggcgcc gatcgcggcg tcggtgcgcg tgagggcttc gaacgcctcg
     9541 tcgggcccgt gcgcatgaga ggcgaagtcg tagtccggca ggtagtagac gaggaagtcg
     9601 aagccgtcac gcgtgacgag ccagcggccc accgcggccg cgtagccgtc gattgagccg
     9661 tgtgaccgtc cgaagacggc gagcggcgcg cccgtgggat ccgactcgaa caggttgtag
     9721 aagaagaagc gcttcggccc gtaagccggg aaggtcagcc ccggaagcgt gggcaggtgg
     9781 ggcgtgcgtc cgcggtagca ggtgatgttg atcgccgccg cggtcagccc ggcgtcttcg
     9841 agcgcttcgt agacggtgac tgcctccgtg ccgagatgaa gccggttcat gttgatggtc
     9901 gtgtcgagga gcgagcgcgt cattcccgcg gcccgcaccg ccccgaacga ggagccgtat
     9961 tcgacgagcc gtgctcgctc gcggtcgtac cagacgaggt gcgggatgcg atggacgtcg
    10021 gggtgcgcac cggtcgcgat cgacgagagg cacactggag tcaatgaggg gaacgtcgag
    10081 atcgcgcgag agtaggctcc ctgctccgcg agaaaagaga gggccggcgt ccggtcgtcg
    10141 ccgaccgccg cctcgaacat cgacggcgtc agtccgtcga tgacgatcag gatgagcgat
    10201 agtggcccgg acgatctcat cggtggatgt gcgggtcgcg agcaccaagc cagaggccgc
    10261 actcaaccgc agcaaggctg tccagccttg ctaagggcga gggtgggctc tggcgccgcg
    10321 tgatcgcggg ccgcacgccg ccgaatgggg tcgttcgggc cactaatctt tggcaagcga
    10381 acgtaacccg gcgtagcgct cgccggctcg gccgcgcagg cgcagggcca atgcaggcag
    10441 gagcgtcgcc tccaagtacc ccgcaaccgg aatccaggcg acggccgcga gcaccactgc
    10501 ggcgagggcg atgaagatcg cggtgccgaa ccgagagccg ccacggcgga gcgcccccga
    10561 gaccagctgc gccgccccga tggagccgag cgcgccgccc acaccgccgc cgacggcttc
    10621 gtcccactgg ccgagggcaa agccgacggc gacaccggcc cctgcggcga ggacgagcgc
    10681 agcggtcagc gcggcgcgtg tgccgatcag gaatcccgcc agcagcacgc cgacggcgac
    10741 gccgagtccc gcgcagacac cgatccagta ccagcttccc atccgtaacc gagtgtagtc
    10801 gcggccctgt tctgcaaacc ggtttccggc tgtctccgta ggtcccgtga cgcccgggtg
    10861 ccactgcccc ggtgcccggg cgccctcgtt cggggcaggc aaggagggca aggggatgag
    10921 acacagggga gtaatcgcga cgctcgcagt cggcgtcgca gcactcggga tcgccgcgtt
    10981 cgcgctggcc ggcggatcgg gtgcaaggtt caaccatctc aaggcgacga tgagcggcta
    11041 ccaggaagtg ccttccgtgt cgtcgaccgg cacggctgac ttcacggccg acgtcagcca
    11101 ggacggaagc tcgattgcct ggcagctcag ctacagcggt ctcgaaggag ccgtccagca
    11161 gtcgcacatt cacttcggac agcggagcgt gaacggcggc atttccgtgt tcctgtgcac
    11221 gaatctcggg aacgggccgg ccggaacgca ggcttgccca caatccggaa cgatcagcgg
    11281 caccatcgac gccgccgacg tgtcaccggc gattcccgcg actgcggcag cccggacgca
    11341 gggcatcgac accggcgagt acgacgagct ggtacgcgcg atcggcgccg gcaaggcgta
    11401 cgcaaacctg cacacggata agtggccagg cggagagatc cgcgcacagt tgaacgagga
    11461 cgacccgcac aactgaggtc gagacgggcg cccggctctc cggccgggcg cccgcgtcag
    11521 cgctgctcga gcctccggat gacggcgagc ccgaactcgg ccgcgcgggc gccgtcgacg
    11581 acgcgatggt cgaacgtgac cgacacgtag ccgatctgcg cggcaacgac ctcgccgtct
    11641 cgcacgaccg gcctcggccc gatgcgtccg atgctgagaa tcgcgacctc ggggtagttg
    11701 acgatgggcg tctgcaagag gccgccgagc ttgcccgcgc tcgtcacggt gaacgttgag
    11761 ccgcgcagct cttccggctt gagcgatccg gcccgcgcgc tttcggcgag gcgggcgaca
    11821 tcggcaccga ggtcttcgat cgaacgcgtg gcgcagtccc ggacgaccgg gacgaccagc
    11881 ccttcgtcgg tctgcacggc gacgccgagg tcgtagcgat ccaggtagac gatctcatcc
    11941 cgctccagac gcgcgttcaa ttccgggaat tcctgcagcg tttcggcgca agccttcaac
    12001 acggtcggga ccagctgctt cagatccagg ccggagaaat cgcactcctc gacccacgtc
    12061 accgcaggca cctctcggtg cgcgcgcgcc atgtgctcgg cgatcacgcg tctcacgccg
    12121 cgcaacggct cgcgcctgcc ctcgctcggc ccagccgtcg cgcgcacgtc gtcctcggtg
    12181 atgcgtccct gtggaccggt gccggcaacg gtgtcgaggt cgacgccgag ctcctgcgcg
    12241 atcttgcgca cgagtggcgt ggcttttcct ttcgtgggac gttcggatgc ggtcgtcttg
    12301 gtcgctgcgc gcggcccttc gccgtcgggc ttgccgccga tgacgacgag caccgtccca
    12361 accgggacga cctttccttc gtcgaccagg atgctggtca cggtccctgc ggcgggtgac
    12421 ggaatctcga ccgtcgtctt gtcggtctgg atctcgacga gcggatcgtc ctccccgatc
    12481 tcctgcccct cggagacgag ccagcgcgcg acctcgcctt ccgtcaatcc ttcgccgaga
    12541 tcgggaagct tgaactcgta ggccacggtc acattcttat cgcgtcgtga gcgaggacgg
    12601 catgcacacc tttgacgccg ttcacgagtt gcgtgacgcg gaggtcgacg acgacgctcg
    12661 caagcgcgag tgcgtcgaca cgttccaggt cccgctcgcg ctgcatcagg tcgagcatcg
    12721 cgtcgacggc gaaggccgcc gcctcgtcga ggtcttcgtc gaaaccgaag gtgagccagg
    12781 cgtcgggcgt ccaggcgatc ggggtcttca actcggtgcg gtcctcgacc gtgagcgtga
    12841 gctcgagccg gtcgagcgga cactcgatcg cggtctgaga gacctccccg tctccctgac
    12901 gcgcgtgtcc atcgcccgcg gagaagagtg cgccgtcgac cggaatcggc aggaagaggg
    12961 tggtgcctgc cacgagctcc ttgcagtcga tgttgccgcc ccaccggcgc ggcggcgcgg
    13021 tcggatgtac tcccggctcg ggtggaggca tgccgaggac gcccgggaag ggtcgcaact
    13081 cgacctcgcg cccctgtgga tcgcggccgg taccggcctc cgagtcgagg ctccagtgga
    13141 gggtgagctt ctcgccttgg ctcatgtcga gccggtcatt gaggggcgtc gaccagccgc
    13201 cggcgagcgt cgcgccgaac gacccgacgc ggacttcgtc gatgcggacc gcgagcgtct
    13261 gtcccgcctt ggccccccgc acctcgatcg ggccgacgag tgcgtgccca gcgtccagct
    13321 cttcgtcgcg aggctcgaat ttctctcccg gcgcgacgtg ccaagccgag tcgagacacg
    13381 agaaggcgac ggtgtcgccg ctctcgatcg tcgcgacggg tgcgaggtcg cgcgagaaat
    13441 gtccgtgcaa cgtgcgccgc tcgagcggaa tctcgtgcgt cacgttcgcg gtactccgat
    13501 ccaggcgtct cgaggcgcaa gcaggacgcg caatccgccg cggctgcctt cttcgaagaa
    13561 ctcccacaac gtgacggcct gccattcgtc cgcctctagc tcgcgggcga tctctgcggg
    13621 gacgtaaccg gcctgcaccc ggcgcgcgct gtcccagacg ccgatcgcgt gtggatcgtg
    13681 ctcgttgtcc ggctcggcca cgagtgcaag ccgccggcct ggagcgaatg cctcgtcctg
    13741 cagcgcttcg atccgatacg acacgccggc gaccttgatc acgcggatgc gcggatcctc
    13801 gccgcgcacg agctcaccgg tcgcggcatc gcgcaggcga aacccagcgc cgctccgctc
    13861 gagccacagc cgcaaacgct cattcgtctg cctttccgac atgccacctg gaccctagtc
    13921 ccggctggcc tcgtcggg
//
LOCUS       DASVEU010000129        13101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_862676,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000129 DASVEU010000000
VERSION     DASVEU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862676"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..530)
                     /locus_tag="VF225_05185"
     tRNA            complement(447..530)
                     /locus_tag="VF225_05185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(494..496),aa:Leu,seq:cag)
     gene            797..3139
                     /locus_tag="VF225_05190"
     CDS             797..3139
                     /locus_tag="VF225_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144684.1"
                     /translation="MTLKAPPLAEVFARQGTLAFSSFARPNRLLLRAPASRDYLLRLD
                     SAQRLLQARIHTRIPAARVRWRFGVVLNGFAVVVPRSQLTTLSRMAGAKVWPSVRYHT
                     LLDRTPQLIGAPTVWGPTLATAGQGMKIAIVDDGIDQTHPFFAPTGYTYPPGFPKGQA
                     SYTTPKVIVARAFAPATPAYANAKLPFDPDLSDHGMHVAGIAAGNNNTLTRSGFRLSG
                     IAPRAYLGNYKALSIPSEFGANGNSPELAAAIEMAVRDGMDVINLSIGEAEIEPTRDV
                     VARALNAAADAGVVSAIAAGNDFGEFGFGSITSPANAAKAISVAASSGGHGSSTVDDV
                     EDFSSAGPTPYSLLLKPDVTAPGDSVLSAAPGGGFVELSGTSMSAPHVAGAAAVLGQR
                     HPTWTPAQIKSALVLTGDPVHNGGSAEVNPLREGGGRIDLVRADQPLVFSSPATLSFG
                     LLKPRAIARRTLSLADAGGGTGVWNVTSSVPGALLSVPAQVTVPGLLPVRVVVPRNAR
                     EGESTGFVILSREGNRRRVPFWFRVERPRLRLDRRIALVRPGEYAGDTRRGAARVSSY
                     RYPEVVPGHAAFPIRLTGREVVYRVHIHGRIANFGVAVIARNRGVGVEPRVVRAGDEN
                     RLAGYTGLPFDQNPYRGSYGRHRLVVGVVLPAPGAYDVVFDTPRGARSGAFRFRFWQG
                     DVQPPSVRVLGARNQYLELAVSDRGSGVDPLSLQVHIDGNVVSASYTSGHARVSLGDI
                     ARGRHALTFTVSDYQETKNMENVARILPNTRTLRTTFVRP"
     gene            complement(3165..3395)
                     /locus_tag="VF225_05195"
     CDS             complement(3165..3395)
                     /locus_tag="VF225_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144685.1"
                     /translation="MGWLMIALLLGTVAAVVGAEWPRLSGRFGNEARLRRERAHRKSQ
                     LKLLRTETEEFAASVERDLNELPTIEETDRKR"
     gene            3439..4716
                     /locus_tag="VF225_05200"
     CDS             3439..4716
                     /locus_tag="VF225_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144686.1"
                     /translation="MRLGLRLVFLATLAALVLPGRAAAATETLVLETSPITVTAFEVA
                     RGVRLAPSPQVDGYVVGMTAEVVDVLGNPVPMQDVMLHHVVFAKVGEPDYTCSGIEDY
                     SGNASSLRAQRFYAEGEEHFALSLPEGYGYPNRASDTWGLLYMLMNHHPHTMVVQIRY
                     TVRYVTREPLTAVKPVWLDVENCRADPVFSVAGTGKPGATVSRTTDFQLPESGRFVAA
                     GGHLHGGGVSLDVSDMSCGSLFTSYPTWGLVFPRPVLHEPGPLHMTGFADPVGRPVRA
                     GDTLRLTATYDNSRPHVRVMGIMILYLAPSPSSSCAAYTSTVPAPSTAERVKVELLKN
                     PTGPLRRNLRGTWVGDYAFGAQRVSLRRGATFTWHFRGGVDHDVTLASGPAGFASPSM
                     RSGTFAHRFTRPGTYRLFCSLHPARMTQLVIVR"
     gene            4750..4911
                     /locus_tag="VF225_05205"
     CDS             4750..4911
                     /locus_tag="VF225_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144687.1"
                     /translation="MSGQLLLPYLALWASLMRALLVRAQIAPPLCARCGLKLERSELG
                     ETICRCGQH"
     gene            complement(4945..6033)
                     /locus_tag="VF225_05210"
     CDS             complement(4945..6033)
                     /locus_tag="VF225_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7144688.1"
                     /translation="MSREEIDRRYGLVRAAMAEQHLDALLVCGSEYTGFEGAVTYMSG
                     FQIVHRYAYVLLPHEGDPTIIFPAEARYVGEHSASWIEDQVFAERPGNYIGERLAAQR
                     VGVYGLDYVMAVRDFEGTHLRVELVPFDVEFDLARAVKSAAEIESVRDSVRINTEGFW
                     AFLEAYRPGRSAAEVLAPAEQLFVEEGCGRLTMNMVLVGAEFALARKDTLLGDFCVPS
                     LEIAGPGGHWVEVSRAIGEPNAEVSRMLEAYEEYFEAAKSALCPGATAHDVHRAVSQG
                     FTDRGYQLGHVTGHSIGMTMIEHPKIGEGIETEVRENMVFSMHPHVIADNGVDCLYMQ
                     DTWLVTTDGGEPLAGLPMRIFRSGEARS"
     gene            complement(6030..6944)
                     /locus_tag="VF225_05215"
     CDS             complement(6030..6944)
                     /locus_tag="VF225_05215"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7144689.1"
                     /translation="MRVCVVGCGAVGSLFAANLAGLDDVDVWAYDLSREHVDAINRDG
                     LRLSGAGEVLGRLTATSDPAALPACDFGIVATKAMHTGAAIEATAAAFVGGCVATVQN
                     GLGNEEAIAKHAERVIRGTTFPAGKLLEPGHVQWDVKGDTTLGPFEPRAAPFEEVERL
                     ADACTRAGMPTTAVRDARGPQWRKVIFNAATNPVGALTRLTHGRVCEDPELRRLVTGL
                     VDEGKAVAAAQGIELDADPEDLIDHAARPEVAYDHKASMLQDVEAHRSTEIDYLNGGI
                     VRFGTEQGVATPLNRAIWSLVKGLEQSW"
     gene            complement(6941..7855)
                     /locus_tag="VF225_05220"
     CDS             complement(6941..7855)
                     /locus_tag="VF225_05220"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7144690.1"
                     /translation="MTVCVIGAGAIGSLFAAHLAKVVDVLVLTRRKEHAQELNDRGLQ
                     VSGRHDFTARVTAAADPDELPEFDLGIIATKANGVEDAASSLAGRFPAATIMTVQNGL
                     GAEEIVHRHGAWPLISAVTFMSGTKHSDTHVEYILDTPTWLGPYGATPFDRVEELARL
                     LVESRLEAEALPDLRPAQWSKLIFNATVNGVAALTGLPHDAHFAAEGAESDLGHLVHA
                     LADEGKRVAVAEGVVLHDDPWEMNVLATRRGSAHYPSMLEDVEARRPTEVEFINGALV
                     REAGRHGVDAPLQNAIYRLVAAKEASYS"
     gene            complement(7852..9336)
                     /locus_tag="VF225_05225"
     CDS             complement(7852..9336)
                     /locus_tag="VF225_05225"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX7144691.1"
                     /translation="MTVGTESPQLERLKAVLAEVADLSHAQQILEWDARVSMPRAGAA
                     ARADVAATVTQLAHGRFVSDDVGKLLADLDAAGHDPDSVEGALIRITRREWERASRIP
                     SELVGEMVQASGVGVAAWDDAKGASDFESFRPHLERQLELKRRYIACFPKTDDPYDVL
                     LDEYEEGLTTAQVEEIFARLKGELVQLVDRHRGDSVEDVQGPFPVERQREAGQLVLAA
                     FGYDSESWRLDETPHPFAAKPGAGDIRLTTHTDERDLTSLFSTMHEFGHGVYEFDIDP
                     SFARTPLGRGTSSAIHESQSRTWENLVGRSPGFWQWFYPQLRGLFPDALGGVDEVSFV
                     RSVSAVRPGLIRGDADEVTYGLHIIMRFELERELIAGTVGVRDLPEVWDARMEESLGV
                     DVPDDAHGVLQDMHWSTGLFGYFPTYQLGNVISAQIWEVARAALGELEEQFSRGEFGP
                     LRRWLSEQIYHHGSRYPPPDLLRRVTGSGIDPEPYLKYLHAKFA"
     gene            complement(9333..10832)
                     /locus_tag="VF225_05230"
     CDS             complement(9333..10832)
                     /locus_tag="VF225_05230"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX7144692.1"
                     /translation="MTNPKFEQLKERLAETQDLTNAATLLSWDQRVMMPPGGAAARAE
                     ATATVSRLAQERFVTDEIGRLLEDLRDLEESSDYDSFEASLIRVTRRDYEKATRVPPE
                     LVGEMSRTSTLALAAWGPAREESNFEALRPHIEKNLELRHRYVECFDAPDETYDVLLD
                     NFEPNMKTAEVREIFDELKEELVPLIQEIGDAGEIDDSFLHGEFDQDAQRVFSFDVLR
                     RFGYSDDEWRLDQTLHPFMTTPGHRDIRLTTNFRADDLSSLFATMHEFGHGVYEWGVD
                     ESLARTPLHSGVSLGLHESQSRTWENLVGRSRSFWRYFYPRLQETFQAQLGSVDEEAF
                     YRGVNRVQPSLIRIDADEVTYNLHIILRFELEQDLIEGRVAVADLPAAWNAKMHEYLG
                     VEVPDDARGVLQDMHWAAGGFGYFPTYALGNVISVQIWERAKEDLGDLDERFEQGEFG
                     ELRDWLREHLYRLGRKFTPQETIQRVTGGPIDAKPYVRYLREKLAPQAV"
     gene            complement(10829..11860)
                     /locus_tag="VF225_05235"
     CDS             complement(10829..11860)
                     /locus_tag="VF225_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7144693.1"
                     /translation="MRRVDSVLQLLAPIALIGVAALVGTIVSTSTETYVINALVNVAI
                     VVALYVFIGNSGVLSFGHISFVAVGAWAAGVLSMPVEEKPAIMPHLASLLRDHTVGNV
                     GSLALATAVGAAYALVVGLPLMRLSGLGAGIATFGVLEITHNVLRYWEKIGPGLNTFS
                     AVPETTGLQQASLGAIVAVVVAFAYRRSRFGRLLSATREDAAAARAVGVSVYRQRLIA
                     FVLSGAVAGFAGGLYVHLLPVNTESVYLDLTFLTLAMLVIGGATSLWGAVVGALAVSA
                     VDSFLAEAESGIHVFGQSLDLPAGTRIVVVGALMALVLILRPAGLTGGRELAFRQRRP
                     LRVLTKFAK"
     gene            complement(11857..12768)
                     /locus_tag="VF225_05240"
     CDS             complement(11857..12768)
                     /locus_tag="VF225_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7144694.1"
                     /translation="MSVAVLGIDWQTLADAIGLGAIYALMAVGIGLVFGVLRLVNFAY
                     GQLVMAGAYALAFASERGWPVSVGIVLCFAVVLALSVLMDQVVFRPLRGQSPAVMLVA
                     TFAVAFLLQSIALLWFGPLGKISASLATLNQPVTISGVNIRKIAIVATVLAAACLLVL
                     VVLLGRTRVGLHMRAAAMDFRTAELLGVRAGRVIGAAVLISGLLAAVVAVMLTVQTPL
                     VTPDFALRDTILVLAGVVLGGMNRLVPATLGGFAIGFASGLLGGALPTDQSQYLPTFL
                     FAAVILVLLVRPGGLFTREGQQAIERV"
BASE COUNT         2163 a         4562 c         4217 g         2159 t
ORIGIN      
        1 cgggcccaac attgaagggg caaccgcgct tctccataat tcgtcccgcg cgtcggcacc
       61 tcagaacggg agccgggacg cggggacctg cggccaggcc cgcgagagcc cggcccctcg
      121 tccacgctaa cgcgccctcg acagactcaa agcactcttt tgacgcacgt tgcgtctttt
      181 ctgaatcgct tgcgcctagc ggcccgaccg ggaccattgc gcttggggcg tcaagagagg
      241 agaaattatg gcgggagagc ggctcacagt tccaggcgag ggagagatcc ccgtaaagcg
      301 gcaagccgac gtgctgtgag tcgttcttct tgtggagccg aacagacgag caggtgccga
      361 cgttccccca aacgagtacg acgcgccggc tacgactgcg tgtggagatc gggtcggcgt
      421 cttccacgga tccgcctgat tggctatgcg gatgagagga cttgaacctc cacggggttg
      481 cccccacacg gacctgaacc gtgcgcgtct accaattccg ccacatccgc gcggacgcac
      541 agtctagcca cgctcccgat tcgctttacg gccgcggcga aggcgggctt cgcccgccgc
      601 agctaacgac cacgtcctgg aggtccgaat cgagtcatgg ccgaaggccg actcgattcg
      661 gctgcgactc gattcggctg cgactcgatt cggtaaaagc catacgcttt ccgacctgag
      721 acggggtctc ctgatctgcg gactgctcgc gctctcgctc gctccggcgg ctcatgccgg
      781 gaacgtcgag gtgctcgtga cgctgaaagc gccgccgctc gccgaggtgt tcgcgcggca
      841 ggggacgctt gccttctcct ctttcgcgcg accgaatcgc ctgctgttgc gtgcacctgc
      901 gagccgcgac tacctgctgc gactcgactc cgcgcagcgc ctcctgcagg ctcgaatcca
      961 cacgaggatt cccgcagcca gggtccgctg gcgtttcggc gtcgtcctca acggctttgc
     1021 cgtcgtcgtc ccacgctcgc agctcaccac gctctcgagg atggcgggcg caaaggtctg
     1081 gccgagcgtc cgctaccaca cgctgctcga ccggacgccg cagctcatcg gcgcgccgac
     1141 ggtctggggc ccgacgcttg cgaccgcagg acaggggatg aagatcgcga tcgtcgacga
     1201 cggcatcgac caaacgcacc cgttcttcgc cccgaccggt tacacgtatc cgccgggctt
     1261 tccaaagggt caggcctcgt acacgacacc caaggtgatc gtcgccagag cgttcgctcc
     1321 tgcaaccccg gcctacgcga acgcaaagct cccgttcgac cccgatctct ccgaccacgg
     1381 catgcacgtt gccggtatag cggcgggaaa caacaacacg ctcacccgga gcgggttccg
     1441 gctctccggg attgccccgc gcgcctacct gggcaactac aaggccctca gcatcccgag
     1501 cgagttcggt gccaacggca actcacccga gctcgccgcc gcgatcgaga tggcggtgcg
     1561 cgacggtatg gacgtcatca atctctcaat cggcgaggcc gagatcgagc cgactcgcga
     1621 cgtggtcgcg agggcgttga acgccgccgc cgacgcgggc gtcgtctcgg caatcgcggc
     1681 cggcaacgat ttcggtgaat tcggcttcgg gtcgatcacc tcaccggcga acgctgcgaa
     1741 ggcgatctcc gtcgctgcat cgagcggtgg ccacggcagt tcgacggtag acgacgttga
     1801 ggacttctcg tctgccgggc caacgccgta ctcgctcctc ctcaagccgg acgtcaccgc
     1861 accgggcgac agcgtgctct cggccgcacc cggcggcggc ttcgtcgagc tcagcgggac
     1921 cagcatgtcc gcgccacacg ttgccggtgc ggcggccgtg ctgggccagc gtcacccgac
     1981 ctggacgccc gcgcagatca agtcagcgct cgtactcacc ggtgaccccg ttcacaacgg
     2041 cggctcggcc gaggtcaacc ccttgcgtga gggtggcggc cggatcgatc tcgtccgagc
     2101 cgatcagccg ctcgtgttct cgagcccggc caccctctcg ttcgggttgc tcaagccacg
     2161 tgcgattgcg cggcgcacgc tcagcctcgc ggacgcaggc ggaggtactg gagtctggaa
     2221 cgtcaccagc agcgttcccg gtgcgctgct ctcggttccg gctcaggtga ccgtgcccgg
     2281 tctccttccc gttcgcgtgg tcgttcctcg gaacgcccgc gaaggtgagt cgaccgggtt
     2341 cgtgatcctc tcgcgcgagg gcaaccggcg cagggttccc ttctggttcc gagtcgagcg
     2401 gccgaggctt cggctcgatc gacgcatcgc gctcgtacgc ccgggcgaat acgcggggga
     2461 cacgaggcgc ggcgccgccc gcgtgtcgtc gtaccggtat ccggaggtcg tgcccggcca
     2521 cgctgccttc ccgatccgac tcaccggacg cgaagtcgtg tatcgcgtcc acattcacgg
     2581 tcggatcgcg aatttcggcg tagcggtgat cgcccgcaac cgcggcgtcg gagtcgagcc
     2641 gcgcgtcgtt cgcgccggcg acgagaaccg gctcgcgggc tacacgggtt tgccgttcga
     2701 ccagaatccg taccgcggca gctacggtcg tcacaggctc gtcgtcggcg tggtcctccc
     2761 cgctcccggt gcgtatgacg tggtcttcga caccccacgt ggcgcgcgct ccggcgcgtt
     2821 tcgcttccgg ttctggcaag gcgacgtcca gcctccttct gtgcgagtgc tcggcgctcg
     2881 caaccagtac ctcgaactgg ccgtcagcga ccgcggctca ggggtcgatc cgctgtcgct
     2941 tcaggttcac atcgacggca acgtcgtctc ggcctcgtac acatccggtc acgcgcgcgt
     3001 gtccctgggc gacattgcac gcgggcggca cgcattgacc ttcaccgtct ccgactatca
     3061 ggagacgaag aacatggaga acgtcgcccg catcctgccg aacacccgga cgctgcggac
     3121 gactttcgtt cggccctagg gtcggacccc gagggccgga ccgcctagcg cttccggtcg
     3181 gtctcttcga tcgtcggcag ctcgttgagg tcgcgctcga cgcttgccgc gaactcctcg
     3241 gtctccgtcc ggagcagctt gagctggctc ttacggtgcg cgcgctcacg gcgaagccgc
     3301 gcctcgtttc cgaatcggcc ggaaagccgt ggccattccg cgccgacgac ggccgcaacg
     3361 gtgccgagta gcagggcgat catcagccag cccacgcggc cattctaccc ttcgcgtacc
     3421 ggtggtagat tcactcggat gcgccttggc ctgcgcctgg ttttcctggc gactctggct
     3481 gcgctcgtcc tccccggccg tgcggcggcc gccaccgaaa ccctcgtgct ggagacatca
     3541 ccgatcaccg tgaccgcctt cgaggtggcg cgaggcgtgc gccttgcccc ctcaccgcag
     3601 gtcgacggat acgtcgtcgg catgaccgcc gaggtcgtcg acgtcctcgg gaatccggtc
     3661 cctatgcagg acgtgatgct ccatcacgtg gtcttcgcga aagtgggcga gccggactac
     3721 acgtgctcgg gcatcgagga ctactcgggc aacgcctcga gcctccgggc ccagcggttc
     3781 tatgccgagg gcgaggagca cttcgccctg tcgctgcccg agggctacgg ctatcccaac
     3841 cgcgcttcgg acacctgggg acttctgtac atgctgatga accatcatcc gcatacgatg
     3901 gtggtgcaga tccgttacac cgtccgctac gtcacgcgcg agccactcac cgccgtcaaa
     3961 cccgtctggc tcgacgtcga gaactgccgc gccgatcccg tcttcagcgt ggcgggaacc
     4021 ggcaagcccg gcgcgaccgt ctcccgcacc accgatttcc agctgccgga aagcggccgt
     4081 ttcgtcgccg ccggtggcca cctgcacggc ggcggtgtct cgctggatgt cagtgacatg
     4141 agctgcgggt cgctgttcac ttcgtatccg acgtggggac tcgtcttccc gcggcccgtt
     4201 ctgcacgagc cgggaccgct ccacatgacg ggcttcgccg atcctgtggg caggcccgtg
     4261 cgggcgggtg acaccttgcg gctcactgcg acgtacgaca actcccgccc tcacgtgcgc
     4321 gtgatgggga tcatgatcct ttacctcgcg cccagcccct cttcgtcctg cgctgcgtac
     4381 acctcgaccg tgcccgcgcc ttccaccgcc gaacgcgtca aggttgagct cctcaagaat
     4441 cccacgggac cgctccgccg caacctccgc ggcacctggg tcggcgacta cgccttcggc
     4501 gcacagcgcg tctcgctgcg gcgcggcgcg accttcacct ggcatttccg cggcggagtc
     4561 gaccacgacg tcaccctcgc aagcggcccc gccgggttcg cgtctccgag catgcggagt
     4621 ggcaccttcg cgcaccgctt cacccgcccc gggacgtacc gcctcttctg ctcgctccac
     4681 ccggcccgca tgactcagct cgtgatcgtc cgctaaaccc gagcggcctc tccgccgata
     4741 acgaacgaca tgagcggcca gcttctcctt ccttacctgg cgctttgggc cagcctgatg
     4801 cgggcgcttc tcgtgcgggc ccagatcgct ccgccactct gcgcgcgctg cgggctcaag
     4861 ctcgagcgct cggagctcgg cgagacgatc tgtcgctgcg gccagcatta gcctcctact
     4921 tctgttccga gccgcccggg tcggttagct ccgggcttcg cccgaccgaa agatccgcat
     4981 cggcaatccg gcgagcggct cgccgccgtc cgtggtgacg agccacgtgt cctgcatata
     5041 gagacagtcc acaccgttgt ccgcgatgac atgcggatgc atcgagaaga ccatgttctc
     5101 tcgaacctcg gtctcgattc cctccccgat cttcggatgc tcgatcatcg tcatgccgat
     5161 cgagtgcccc gtcacgtgac ccagttgata gccgcggtcg gtgaagccct gcgagaccgc
     5221 gcggtgcacg tcgtgagccg tcgccccggg acagagagcc gactttgccg cctcgaagta
     5281 ttcctcgtag gcctccagca tccggctcac ctcggcgtta ggctcgccga tcgcgcgcga
     5341 gacctcgacc cagtggccgc cgggaccggc gatctcgagc gacgggacgc agaagtcacc
     5401 gaggagggtg tccttgcgag cgagcgcaaa ctccgccccg acgaggacca tgttcatggt
     5461 cagccggcca cagccctcct ccacgaagag ctgctccgcg ggagcgagca cctccgccgc
     5521 cgacctgccg ggccggtacg cctccaggaa cgcccagaag ccctcggtgt tgatgcgaac
     5581 cgagtcgcgc acggattcga tctccgctgc gctcttcacc gcgcgtgcca ggtcgaactc
     5641 gacgtcgaac ggcacgagct cgacgcggag gtgcgtgccc tcgaagtccc ggacagccat
     5701 cacgtagtcg aggccgtaca cgcccacgcg ctgagcggcc aggcgctcgc cgatgtagtt
     5761 gccgggccgc tccgcgaaga cctgatcctc gatccacgaa gcgctgtgct ccccgacgta
     5821 gcgcgcctcg gccggaaaga tgatcgtcgg gtcgccctcg tgcggcagca agacgtacgc
     5881 gtagcgatgg acgatctgga agccggacat gtaggtgacc gcgccctcga agccggtgta
     5941 ttcggagccg cagacgagca acgcgtccag gtgttgctcc gccatcgccg cgcggactag
     6001 gccgtagcgg cggtcgattt cctctcggct caccatgact gctccaaccc tttcacgagc
     6061 gaccagatcg cacggttcaa cggtgtcgcg acgccctgct ccgtaccgaa acgcactatg
     6121 ccgccgttca agtagtcgat ctccgtcgaa cggtgcgcct cgacgtcctg gagcatcgac
     6181 gccttgtggt cgtaggcgac ctccggcctg gccgcgtggt cgatcaggtc ctccgggtcg
     6241 gcgtcgagct cgatcccctg cgccgcggcg accgccttgc cctcgtccac caggccggtg
     6301 acgaggcgcc gaagctcagg gtcctcgcac acgcgcccgt gcgtcagccg cgtgagcgcg
     6361 ccgaccggat tcgtcgccgc gttgaagatc accttccgcc actgtggacc gcgcgcgtcg
     6421 cgcacggccg tcgtcggcat cccggctcgg gtacaagcgt cggcgagtcg ctctacctcc
     6481 tcgaacggag ccgcgcgcgg ctcgaacggc ccgagtgtcg tgtcgccctt gacgtcccac
     6541 tggacgtggc ccggctcgag cagcttgccg gcaggaaagg tcgtgccacg aatcacccgc
     6601 tcggcgtgtt ttgcaatcgc ttcctcgttt cccaggccgt tctggaccgt cgcgacacaa
     6661 ccgcccacga acgcggcagc cgtcgcctcg atcgcagccc cggtgtgcat cgccttcgtc
     6721 gcgacgatgc cgaagtcgca tgccgggagc gcggcggggt ccgaggtcgc cgtcagtcgg
     6781 cccagtacct cgcccgcccc gctgaggcgc agtccatcac gattgatcgc gtcgacgtgc
     6841 tctctcgaga ggtcgtaggc ccagacgtcg acgtcgtcga gccctgcgag gttcgcggcg
     6901 aagagcgagc cgaccgcccc acacccgacg acgcagaccc tcacgagtag gacgcctctt
     6961 tggccgcgac gagtcggtag atcgcgttct gcagaggagc gtccacccca tgacgccctg
     7021 cctcccggac gagcgcgccg ttgatgaact caacctcggt cggtcgccgc gcctcgacgt
     7081 cctcgagcat cgacggatag tgcgcgctcc cgcgcctggt ggcaaggacg ttcatctccc
     7141 acggatcgtc gtgcagcacg acaccctctg ctacggcgac acgtttgccc tcgtccgcga
     7201 gcgcgtggac gaggtggccg agatcggact cggcaccctc ggcggcgaag tgcgcatcgt
     7261 gcggcagccc ggtcagggcg gcgaccccgt tgacggtcgc attgaagatc agcttcgacc
     7321 actgggcggg acgcaagtcg ggcagagcct ccgcctcgag cctcgactcg accagaagcc
     7381 gggcgagctc ctcgacgcga tcgaacggcg tcgccccgta cggcccgagc cacgtcggcg
     7441 tgtcgaggat gtattccacg tgcgtgtcgg agtgcttggt cccgctcatg aacgtcactg
     7501 ccgagatcag tggccacgcg ccgtggcgat ggacgatctc ctccgcacca agaccattct
     7561 ggacggtcat gatcgtcgcg gcagggaagc ggccggccag cgacgacgcc gcgtcttcga
     7621 cgccatttgc cttcgtcgca atgatcccca aatcgaactc ggggagttcg tcagggtcgg
     7681 ccgccgctgt cactcgcgcg gtgaagtcgt gccggccgct gacctggagt ccccggtcgt
     7741 tcagctcctg cgcgtgttct ttgcgacgcg tgagcacgag cacgtccaca acctttgcga
     7801 ggtgcgcagc gaagaggctg ccaatggctc ctgcaccgat gacgcagacc gtcacgcgaa
     7861 cttcgcgtgg aggtacttca gatacggctc gggatcgatg ccggatccgg tcacacgacg
     7921 cagcaggtca ggcggtgggt accggctgcc gtggtggtag atctgctcgc tcaaccaccg
     7981 gcgcagcggc ccgaattcac cgcgggaaaa ctgctcctcg agctcgccga gcgcggcgcg
     8041 cgcgacctcc cagatctgcg ccgagatgac gttgccgagc tgataggtcg ggaagtaccc
     8101 gaacaggccg gtcgaccagt gcatgtcctg gagcacgccg tgcgcgtcgt ccggaacgtc
     8161 gacaccgagt gattcctcca tccgagcgtc ccagacctcc ggcaaatcac gaacgccaac
     8221 ggttccggcg atcagctccc gttcgagctc gaaccgcatg atgatgtgga gcccgtaggt
     8281 cacctcgtcg gcatcgccgc gaatgagtcc gggccgcacc gcgctgaccg agcggacgaa
     8341 cgagacctcg tcgacaccgc cgagcgcgtc ggggaacagc cctcgcagct gcggatagaa
     8401 ccactgccag aaacccgggc ttcgtccgac gaggttctcc cacgtccggc tctgcgattc
     8461 atggatcgcg ctggacgtcc cccggccgag cggcgtcctt gcgaacgacg ggtcgatgtc
     8521 gaactcgtag accccgtgcc cgaactcgtg catcgtcgaa aagagtgacg tgagatctct
     8581 ctcgtccgtg tgagtcgtca gtcggatgtc cccggcgccc ggcttggccg cgaacgggtg
     8641 cggcgtctcg tcgagccgcc aggattccga gtcgtagccg aacgccgcca gcaccagttg
     8701 ccctgcctcg cgctgccgtt cgacgggaaa cgggccttgg acatcctcga ccgagtcgcc
     8761 gcgatggcgg tcgacgagct gcacgagctc gcctttgagc cgcgcgaaga tctcctcgac
     8821 ctgtgcggtc gtgaggccct cttcgtactc gtcgaggaga acgtcgtagg gatcgtccgt
     8881 cttgggaaag catgcgatgt agcggcgctt cagttcgagt tgacgctcga gatgaggcct
     8941 gaaggattcg aagtccgacg cgcctttcgc gtcgtcccac gcggctacac ccacgccgct
     9001 cgcctggacc atctcgccga cgagctcgga cgggatcctg ctcgctcgct cccactcgcg
     9061 ccgggtgatc cggatcagtg ctccctcgac cgagtccgga tcgtgaccgg cggcgtccag
     9121 gtcggccaac agcttcccca cgtcgtccga gacgaagcgc ccgtgcgcga gttgcgtcac
     9181 tgtcgctgcc acgtctgcac gagccgcggc gcccgcgcga ggcatcgaca cgcgggcgtc
     9241 ccattcgagg atctgttgcg cgtgagagag atccgcgacc tctgccagga cggccttcag
     9301 ccgttcgagc tgcggcgatt cggtcccgac cgtcaaacgg cctgaggggc gagcttttcg
     9361 cggaggtacc gcacgtacgg tttcgcgtcg atcggtcctc cggtgacgcg ctggatcgtc
     9421 tcttgcggcg tgaacttgcg ccccagccgg tagaggtgct cccgcagcca gtcgcgcagc
     9481 tcgccgaatt caccctgctc gaagcgctcg tcgaggtcgc cgaggtcttc cttcgcccgc
     9541 tcccagatct gcaccgagat cacgttcccg agcgcgtagg tggggaagta tccgaacccg
     9601 cccgccgccc agtgcatgtc ctggagcacg ccgcgcgcgt cgtcgggaac ctcgaccccg
     9661 aggtactcgt gcatcttcgc gttccaggcc gccggcaggt cagcgacggc gacgcggcct
     9721 tcgatcaggt cctgctcgag ctcgaagcgg aggatgatgt gcaggttgta ggtcacctcg
     9781 tccgcgtcga tgcggatgag ggacggctgc accctgttca cgccccggta gaacgcctct
     9841 tcgtccacgg agcccaactg cgcctgaaag gtctcctgca gccgcggata gaagtaccgc
     9901 cagaacgagc ggctgcgccc gaccagattt tcccaggtcc tgctctgaga ctcgtgcagg
     9961 ccgagcgaca ctcccgagtg aagcggtgtt ctcgcaagtg actcgtcgac tccccactcg
    10021 tacaccccgt gaccgaactc gtgcatggtg gcgaaaagcg agctgagatc gtcggcacgg
    10081 aagttcgtgg tgagccggat gtcgcggtgg cccggagtcg tcatgaatgg atgcagggtc
    10141 tggtcgagcc gccactcgtc gtccgagtag ccgaaacggc gcagcacgtc gaagctgaag
    10201 acccgctgtg cgtcctggtc gaactccccg tgcaggaacg agtcgtcgat ctcccctgcg
    10261 tcgccgatct cctggatcag cggcacgagc tcctccttga gctcgtcgaa gatctcacgc
    10321 acctccgccg tcttcatgtt cggctcgaag ttgtcgagga ggacgtcata cgtctcgtcg
    10381 ggcgcatcga agcactcgac atagcggtgg cgcagctcca gatttttctc gatgtgcggt
    10441 cgcagagcct cgaagttcga ctcttccctt gcgggccccc aggccgcgag agcgagcgtc
    10501 gacgtccgac tcatctcgcc tacgagctcc ggcgggacgc gggtggcctt ctcgtagtcg
    10561 cgccgcgtga cgcggatcag actcgcttcg aaggagtcgt agtccgagct ctcctcgagg
    10621 tcgcgcaggt cttcgagcag gcgcccgatc tcgtccgtga cgaatcgctc ctgcgcgaga
    10681 cggctgaccg tcgccgtcgc ttccgcgcgg gccgcggcgc cgcccggggg catcatcacg
    10741 cgctggtccc aggacagcag ggtggcggcg ttcgtcaggt cctgcgtctc ggcgagccgc
    10801 tccttcagtt gctcgaactt cggattggtc atttggcgaa ctttgtcaga acgcgcaggg
    10861 gccggcgctg acggaatgcg agctcccggc cgccggtgag ccccgccggg cggaggatca
    10921 ggacgagggc catgagggcc ccgacgacca cgatccgcgt accggccggg aggtcgagcg
    10981 actgtccgaa gacgtggatg ccgctctcgg cctcggcgag gaacgagtcg acagcactga
    11041 cggccagcgc tcccaccacg gcgccccaga ggctggtcgc gccgccgatc accagcatcg
    11101 ccagcgtgag gaaggtgagg tcgagataga cggactcggt atttacgggg agcaggtgga
    11161 cgtagagacc gcccgcgaac cctgccaccg cgcccgagag cacgaacgcg atcagccgct
    11221 ggcggtacac cgacacgccg acggcgcgcg ccgcggccgc gtcttcgcgc gtcgcgctca
    11281 ggagcctgcc gaaccggctg cgtctgtacg cgaaggcaac gaccacggcc actatggctc
    11341 ccagcgaggc ttgctggagc ccggttgtct cggggaccgc ggagaaggta ttcagtcccg
    11401 gcccgatctt ctcccagtag cgcaggacat tgtgcgtgat ctcgaggacg ccgaacgtcg
    11461 cgattcctgc tcccaggccg gacagacgca tcagcggcag gccgacgacg agcgcgtacg
    11521 ccgcaccgac ggcggtggcg agcgcgagtg acccgacgtt gccgaccgtg tgatcgcgaa
    11581 gcaggctcgc gaggtgcggc atgatcgcgg gcttttcctc cacgggcatc gagagcacac
    11641 cggcggccca cgcgccgacc gcgacgaagc tgatgtgtcc gaacgagaga acgccggagt
    11701 tgccgatgaa gacgtagagc gcaacgacga tggcgacgtt cacgagcgcg ttgatcacgt
    11761 aggtctccgt cgacgtcgag acgatcgttc cgacgagcgc ggcgacgccg ataagcgcga
    11821 tcggcgcgag caactggaga acagagtcga ctcgcctcat acccgctcga tcgcctgttg
    11881 gccttcacgc gtgaagaggc caccgggtct gaccagcaga accaggatga cggccgcgaa
    11941 caggaacgtg ggcaggtact gactctggtc tgtcgggagc gcacctccga ggagcccgga
    12001 cgcgaaaccg attgcgaacc ccccgagggt cgcagggaca agccggttca tgccgcccag
    12061 cacgacgccc gccaggacca gaatcgtgtc gcgcagggcg aaatccggcg tgacgagtgg
    12121 cgtctgcacg gtgagcatca ccgctaccac cgctgcaagc aagcccgaga tcagcacggc
    12181 agcgccgatc acgcgcccgg cccgcacgcc gaggagctcg gcggtgcgaa agtccattgc
    12241 ggcggcacgc atgtgcagcc cgacgcgggt gcggccgagc agtacgacga gcaccagcag
    12301 gcaggcggcc gccagcaccg tcgcgacgat cgcgatcttg cgaatgttca cgcccgaaat
    12361 cgtgaccggc tgattcaacg tcgccagaga ggccgaaatc tttcccagcg gtccgaacca
    12421 caacagggcg atgctctgca agagaaaggc gaccgcgaac gtggcgacga gcattacggc
    12481 cggcgactgc ccgcgcagcg ggcgaaagac gacctgatcc atgaggaccg agagggcgag
    12541 cacgaccgca aaacagagga cgatgccaac ggagacgggc cagccgcgct cggaggcgaa
    12601 tgcaagcgcg tacgcgccgg ccatcaccag ctggccgtag gcgaagttga cgagacgcag
    12661 gacgccgaag acgaggccga tgccaacggc catgagcgcg tagatcgcgc cgagaccgat
    12721 cgcgtcggcg agcgtctgcc agtcgatgcc gaggaccgcg acgctcatcg gctgcctttc
    12781 ggccgatgag cggtggaagg ggcgtaaggg gaaaccggga ggtttccccc cgccttctcc
    12841 tcagcccacg cggcgatctc agcggcgcgg gggctgaggc gatccctaaa gaagggagct
    12901 cgtgagggaa acatggtttc cctcacggga gcgagccgaa ggcgagcgac gctcatgagt
    12961 acctgatccg cgggttgatg atcgcgtacg agatgtccac gatcaggttg acgaacacga
    13021 agacgagcgc aaggaagagg atcccgccct gcaagaccgg gtagtcacgg ttgaagatgg
    13081 cgtcgcgcag ccacgatccc a
//
LOCUS       DASVEU010000130         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_282231,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000130 DASVEU010000000
VERSION     DASVEU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282231"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1132
                     /locus_tag="VF225_05245"
     CDS             56..1132
                     /locus_tag="VF225_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7144695.1"
                     /translation="MSVAGAALLEVEHLEVRFPVRSGVLVQRTVASVHAVDDVSFSLG
                     EGETLGIVGESGCGKTTLVRALMRLADSTGGAIRFRGRDITRAGRRELAAVRRQMQMV
                     FQDPQSSLNPRKRVSQIVGTPLRLSGAADGEDVDARTRALLATVGLNPEHLNRFPHEF
                     SGGQRQRIGIARALAVQPKLIMLDEPVSALDVSIQAQVVNLLGELQDALGLSYVFVAH
                     DLSVVRHVSDRIAVMYLGKIVELSPAERLYDKPIHPYTVALLGAIPIPDPRQNRARPR
                     TIVEGEPPSPLAPPPGCRFHTRCAHATDRCREVEPPLMEYAGGHLAACHHPRNVSAAE
                     TADALRSPASPLSAGAMDGHVETR"
     gene            complement(1114..1755)
                     /locus_tag="VF225_05250"
     CDS             complement(1114..1755)
                     /locus_tag="VF225_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7144696.1"
                     /translation="MARVRFQISMSLDGFIAGPNQSEENPLGEGGMRLHDWAFGLAAW
                     RAPHGQEGGEVNASSEVVERSLENIGATVMGRNMFGGRGAWGDEPWDGWWGDEPPFHM
                     PVFIVTHHYREPVVKQAGTTFTFVTDGIESALEQARQAAGAKDVALAGGANVAQQFLA
                     AGLIDEMTIHLVPVLLGDGARLFDDLDGVDVELQCIRAIEGPAVTHLTYRVST"
     gene            1949..2722
                     /locus_tag="VF225_05255"
     CDS             1949..2722
                     /locus_tag="VF225_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7144697.1"
                     /translation="MDAVFKALSDPTRRSLLDELFEQDGQTLSALEQRLPMTRFGVMK
                     HLRVLEEAGLVVARRRGREKLHFLNPVPIRLVHDRWVSKYAEPWAAGLSELKTRLEQT
                     MEKVFEIYIRTTPQRLWEAITDPAMRSRYTFGVHVDSDWTPGSSYVGTHPAAPAALLE
                     GENLEVDPPKRLVQSYMAMWSEDVQRAGTSRVTWEIEAVGDSCRLMVTHDQLPEDAND
                     QIYGGWPQILSGLKTLLETGETLTTPGSLRYSQASAPPA"
     gene            complement(2792..2998)
                     /locus_tag="VF225_05260"
     CDS             complement(2792..2998)
                     /locus_tag="VF225_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144698.1"
                     /translation="MASATRIGTAGLQGWRRKVGDVVAEPLAQRTPLASEQIRAAVGA
                     LFFVLSVMYVVKTLSAATRELRES"
     gene            3239..3463
                     /locus_tag="VF225_05265"
     CDS             3239..3463
                     /locus_tag="VF225_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7144699.1"
                     /translation="MQVNMHDAKTRLSELVAAAESGHEVIIARDGTPAVRLVAVGVRY
                     PPVRLGALAGQIEIGEDFDEPLPEFQPYTA"
     gene            3460..3834
                     /locus_tag="VF225_05270"
     CDS             3460..3834
                     /locus_tag="VF225_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7144700.1"
                     /translation="MSGVLVDTHIALWALGNPTHLSDTERALLVDPSVDRRLSPVSIW
                     EAAIKREAGRLRAPRNLAAVLAAQFRMLDVTASLLEAAAELPRLHADPFDRVLIAHAL
                     LEDLAVLTRDRAFAAYGARLAA"
     gene            4296..5291
                     /locus_tag="VF225_05275"
     CDS             4296..5291
                     /locus_tag="VF225_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7144701.1"
                     /translation="MARSDLLKQMFASYQQGDDAGFREAASEVITNEREKRHGLLADE
                     LQRILDIAPAGAERPRQISSLRPLPTSRGDAPLIEAFSPQRQLDAQTLRPDVRATLDQ
                     IVEEQTRADVLRAHGLSPSRKLLFVGPPGTGKTTTAEAVAGEIGLPFARVQLPAVVSS
                     LLGETARNVSAVFDFCRREHWVLLFDEFDALGKERADEAEHGELKRVVTVFLQLLDEF
                     AGPGLVIAATNHPAMLDEAVWRRFDEVVGFALPNQRELEKLVRRLFRRIRLSISGTDV
                     ARRLKGMSHSDAESTIRTAMKLSVLRDGDAVTAADIDAALRRQETRRATIHSSRV"
     gene            5519..5884
                     /locus_tag="VF225_05280"
     CDS             5519..5884
                     /locus_tag="VF225_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144702.1"
                     /translation="MASETWRDADLLELDAATAGAAVAFSSDPQLAAFLQRLAKHRAA
                     KETKSGHLFAADLFDAVESIRRYGPNDRLSLRLVEYLASTTENILASTWNSGAQGTTS
                     RWRTNGASMSNERSQRPTG"
     gene            5794..6279
                     /locus_tag="VF225_05285"
     CDS             5794..6279
                     /locus_tag="VF225_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7144703.1"
                     /translation="MELWRPGDDVALADKWRVDVERAIAEADGLTVDRYVDHPRGIVL
                     LRVRARPKIIGEIAGIDEIATIDLVPQAPPVLMSALEASLDEIPEADAPSEDAPLVAV
                     VDSGINASHPLLVKSVYEAITLLHELSDGTDDHGHGTAVAGVLVNGPSEDCWLSGGDR
                     R"
BASE COUNT         1038 a         2192 c         2193 g         1000 t
ORIGIN      
        1 cgcggcggga ggtcggccag gagatcggcc tagcgttcgg agagacggtg atctcgtgag
       61 cgtggccggc gccgcgctgc tcgaggtcga gcacctcgag gtgcgcttcc cggtgcgatc
      121 aggagtcctg gtgcaacgca cggtggcctc ggtgcacgcg gtcgacgacg tctcgttctc
      181 cctcggcgag ggcgagacgc tcggcatcgt gggcgagtcg ggctgcggca agacgacgct
      241 cgtccgcgcg ctgatgcggc tcgccgacag cacgggtggc gcgatccgct tccgcgggcg
      301 cgacatcacg cgcgcgggac ggcgcgagct ggcggccgtg cggcgccaga tgcagatggt
      361 cttccaggac ccgcagtcgt cgctgaaccc gcgcaagcgc gtgagccaga tcgtcgggac
      421 gcccctgcgc ctgagcggcg cggcggacgg cgaggacgtc gacgcacgca cgcgggcgct
      481 gctcgcgacc gtcggcctga accccgagca cctcaaccgc ttcccgcacg agttctccgg
      541 cggccagcgc cagcggatcg ggatcgcccg ggcgctcgcc gtgcagccga agctcatcat
      601 gctcgacgag ccggtgtccg cgctcgacgt gtcgatccag gcgcaggtcg tcaacctcct
      661 cggcgagctg caggacgcgc tcggcctgag ctacgtcttc gtggcccatg acctgtcggt
      721 cgtgcgacac gtcagcgacc ggatcgcggt catgtacctg ggcaagatcg tcgagctgtc
      781 gcccgccgag cggctctacg acaagccgat ccacccgtac accgtcgcgc tgctcggtgc
      841 gatcccgatc cccgacccgc gccagaaccg cgctcgcccg cgcacgatcg tcgaggggga
      901 gccgccgagc cccctggcgc cgccgcccgg atgccgcttt cacacgcgct gcgcccacgc
      961 gaccgaccgc tgtcgcgagg tcgagccgcc gctcatggag tacgccggcg ggcatctggc
     1021 ggcctgccat cacccgcgca acgtgagcgc cgcggagacg gcggacgcgc tgcgctcacc
     1081 ggcgagcccg ttgagcgcgg gcgcgatgga tggtcacgtg gagacgcggt aggtgaggtg
     1141 ggtgacggcg ggcccttcga tcgcgcggat gcactgaagc tcgacgtcga cgccgtcgag
     1201 atcgtcgaac agccgggcac catcccccaa gaggaccggc acgaggtgga tcgtcatctc
     1261 gtcgatcagt cccgccgcga ggaactgctg cgcgacgttc gcgccgccgg cgagcgcgac
     1321 gtccttggcg ccggccgctt gccgagcctg ctcgagcgcc gactcgatgc cgtccgtcac
     1381 gaacgtgaag gtcgtgcccg cctgcttgac gaccggctcg cggtagtggt gggtgacgat
     1441 gaagaccggc atgtgaaacg ggggctcgtc gccccaccag ccatcccacg gctcgtctcc
     1501 ccacgcgccg cggccgccga acatgttgcg gcccatcacc gtcgctccga tgttctcgag
     1561 cgatcgctcg acgacctcgc tcgaggcgtt gacctcgccg ccctcctggc cgtggggcgc
     1621 gcgccaggcg gccagcccga aggcccagtc gtgcagccgc atgccgccct cgcccagcgg
     1681 gttctcctcg ctctggttgg ggcctgcgat gaagccgtcc agcgacatcg agatctgaaa
     1741 cctcactctg gccatggtgc ctcccttagc ttggtggtcc gacgatgaga ccgctcgggg
     1801 ggcggcgaac tcatcgccag gccgagcgcg gcgccctgag cgagcgctgg gcggcccgcg
     1861 cagagggtgt gcccaaaggc ctcggacatc gcgcccgtcc aagccccgat gattgacagg
     1921 tgacccaacg gtcacgtatt ctgcgcacat ggacgcggtg ttcaaggcgc tctccgaccc
     1981 gacgcgacgg agcctgctcg acgagctctt cgagcaggac gggcagacgc tcagcgcgct
     2041 cgagcagcgg ctgccgatga cgcgcttcgg cgtcatgaag cacctgcggg tgctcgagga
     2101 ggcagggctc gtggtcgcgc gcaggcgagg gcgcgagaag ctccacttcc tgaacccggt
     2161 cccgatccgg ctcgtccacg accgctgggt gagcaagtac gcagagccct gggccgcggg
     2221 gctcagcgag ctcaagacca gattggagca gaccatggag aaggtgttcg agatctacat
     2281 tcgcacgacg ccgcagcgcc tctgggaggc gatcaccgac cccgcgatgc gcagcaggta
     2341 cacgttcggg gtccacgtcg actcggactg gacccccggc tccagctacg tgggcaccca
     2401 tccggcagcg cccgcagcgc tcctcgaggg cgagaacctc gaggtcgacc cgccgaagcg
     2461 gctcgtgcag agctacatgg cgatgtggag cgaggacgtc cagcgcgcgg ggacgtcgag
     2521 ggtcacctgg gagatcgagg cggtcggcga ttcatgccgg ctcatggtca cccacgatca
     2581 gctgcccgag gacgccaacg accagatcta cggcggctgg ccgcaaatcc tctcaggcct
     2641 gaagacgctg ctggagaccg gcgagacgct cacgaccccg ggctcgctgc gctactcgca
     2701 ggcgtccgca cccccggctt gaaaacgccg gcggacctgg tcgaggcgtc gcggcggcgt
     2761 ctcggccggg cccgccgagc gccggagcgg atcagctctc gcgcagctcg cgcgtcgcgg
     2821 cgctcagcgt cttgacgacg tacatcacgg agagcacgaa gaacagcgcc cccaccgccg
     2881 ccctgatctg ctcgctggcc agcggggtgc gctgcgcgag tggctcggcc acgacgtcgc
     2941 cgaccttgcg ccgccaaccc tgcagtcctg cggtcccgat cctggtcgct gatgccatgc
     3001 tcttcctcct gcgtcgctgt cgagggatgg ttccccaaca agcgccattt tgatcgcgca
     3061 ccgtcgctcg gcgtgcggcc cgcgctcgcg gcggcacgtg cgacgagcat cccggtcgct
     3121 tgcgtatcgc tcgatctccg agcgcccgcg cgcgtaggag gcggtgctca cgagcagggc
     3181 cccggtctct cgcgcaggtc ttgcgtctcc gagcctgcct agctaccatc gctagctaat
     3241 gcaggtcaac atgcacgacg ccaagacccg gctcagcgag ctggtcgccg cggccgagag
     3301 cggccacgag gtgatcatcg cgcgggacgg aacgccggcg gtacgacttg tcgccgtcgg
     3361 cgtgaggtat ccgccggtgc gcctgggtgc gctggccggc cagatcgaga tcggcgagga
     3421 cttcgacgag ccgctgcctg agttccagcc gtacaccgca tgagcggcgt tctcgtcgac
     3481 acgcacatcg cgttgtgggc gctgggcaac ccgacgcatc tgagcgacac agagcgagcg
     3541 ctgctcgtcg acccgtccgt cgaccgccgt ctgagccctg tttcgatctg ggaggccgcg
     3601 atcaagcgtg aggccggacg gttgcgcgcg ccacgcaacc tggccgccgt tctcgccgcc
     3661 caattccgca tgctcgacgt cacggcctcg ctgctggagg cggctgccga gctgccccgc
     3721 ctgcacgccg atccgttcga ccgcgtgctg atcgcgcacg cgctactcga agacctcgcc
     3781 gtgctgacac gtgaccgcgc cttcgccgcc tacggcgcga gacttgcagc ctgagagcca
     3841 tcgggacggt gtcgcccggc ggcgctgatg cttcgcgcga gaagcgaaac gctgcccaga
     3901 cgtcggatgc ttcgacgagg gactcggcaa gcgacggaac agcacgggag cccgccggag
     3961 acacggccca cgctctccgg cgacggccca tcggggagac gcgacatcga cccaccgaac
     4021 cgactcctgt tcgggcaaca cccgcgccgc ctcacgcggt cccgcccatg ccccggtccg
     4081 gacgtcctgc gcctcccgtc acccgtggat gcggagcgcg tcacggaagt cgcgtatctt
     4141 catacgcgtc ccggcgctgt ggagtgcggt ccggcgcgat ccagagcccg cggctccggc
     4201 agacgagcaa acgttccaag cgacaaggaa gcgacagctt ccgccgtcta ccggtcgtcc
     4261 gccccgttgt ctccgcagac acgtaggctt cgcccgtggc ccgaagcgac ctcctcaagc
     4321 agatgttcgc gagctaccag cagggtgacg acgctggctt ccgcgaggcc gcgtccgagg
     4381 tcatcaccaa cgagcgcgag aagcgccacg gtctattggc cgacgagctt cagcgcatcc
     4441 ttgacatcgc accagccggc gctgaacggc cgcgccagat ctcgagtctg cggcccctcc
     4501 cgacctcgcg tggtgacgca ccgttgatcg aggccttctc tccccagcgg caattggacg
     4561 cgcagaccct gcgtcccgac gtccgcgcga cgctcgatca gatcgtggag gagcagaccc
     4621 gcgcggatgt gttgagggcg catggattgt cgccgagccg gaagctgttg ttcgttggac
     4681 caccggggac cggcaagacg acgaccgccg aggcagtcgc gggcgagatt ggcctgccgt
     4741 ttgctcgcgt gcagctgccc gcggtggtct cgtcgctgct gggcgagacg gcgcgcaacg
     4801 tgtcggcggt gttcgacttc tgccggcggg agcactgggt cctcctcttt gacgagttcg
     4861 atgcgcttgg gaaggaacgt gctgacgagg ccgaacacgg cgaattgaaa cgggtcgtca
     4921 ctgtgttctt gcagctactt gatgagttcg ccggtccagg cctggtcatc gcggcgacaa
     4981 accatccggc gatgctcgat gaggcggtct ggcgccgatt cgacgaggtc gtcgggttcg
     5041 ccctgccgaa tcagagggaa ttagagaagc tagtgcggcg actgttccga cggatccggc
     5101 tatcgatctc ggggaccgac gtcgcccgac gactcaaggg aatgtcgcat tccgacgccg
     5161 agtcaaccat ccgaacggcg atgaagctgt ccgtcctgcg agacggcgat gctgtgaccg
     5221 cggccgacat tgatgcggcc cttcgccgac aggagacaag acgagcgacc atccacagct
     5281 cccgggtgta attcccgagc cgccgaggct tgaaaatctc gcgctgattg aacttcgccg
     5341 cgggccgcgg gttgttccac gcggtggcgg cggagggaag cgaaagcaca aagatcatca
     5401 ggcccacgcc gacgtcctca gggcgcaggc tgaaaatgtt cgcgccgaac aactcaagcg
     5461 cccagctacg aggggaatcg atccgaagct gatcttggta ctggcactga atcgacacgt
     5521 ggcgtccgag acctggcgtg acgcggacct cctcgagttg gacgctgcca cggcgggcgc
     5581 ggcggtggcg ttctcctcag acccgcagct cgccgcgttc ctccagcggc ttgcaaagca
     5641 ccgcgcggcg aaggagacca agagcggcca cctttttgca gccgatctgt ttgatgcagt
     5701 cgagagcatc aggcgctacg gccccaatga ccgcctctcc cttcgtctcg tcgagtacct
     5761 cgcttcgact accgagaata tactcgcatc gacgtggaac tctggcgccc aggggacgac
     5821 gtcgcgttgg cggacaaatg gcgcgtcgat gtcgaacgag cgatcgcaga ggccgacggg
     5881 ctaaccgtcg atcgctatgt ggatcatccc cgagggatcg ttcttctgag agtgcgagcg
     5941 cggccaaaga tcatcggtga gatcgcggga atcgatgaaa tcgccacgat cgacctggtt
     6001 ccacaggcgc cgccggtact catgtcggcg ctggaggcga gtctcgatga gatcccggag
     6061 gctgacgcac cgagcgagga cgcgcccttg gtagccgttg tggactcggg catcaacgcg
     6121 agccatccac tgctggtcaa gtccgtctac gaagcgatca cgctacttca cgagctttca
     6181 gacggaactg acgatcacgg tcacggcacc gctgtcgcag gcgtactggt gaacgggcca
     6241 tccgaggatt gctggctctc cgggggtgat cgtaggtgag tgatgtggtc tgttcgtgcg
     6301 ggagagtgag tgtggtcggg ccgcaggtca ggtggatcag ggcgagcgcg gcgttggcgg
     6361 agtgaaagcc gtaggcgcgg cgggtgatca gcttggcctt gttgttgagc gcttcggcgc
     6421 ggg
//
LOCUS       DASVEU010000131         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_863114,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000131 DASVEU010000000
VERSION     DASVEU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863114"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..676
                     /locus_tag="VF225_05290"
     CDS             128..676
                     /locus_tag="VF225_05290"
                     /inference="COORDINATES: protein motif:HMM:NF040425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HEX7144704.1"
                     /translation="MIAAQAARIDELVAQNQTLAARVAELERLAGRSSRNSSLPPSKD
                     SPQARRERPRKQTQRKHGGQQGHPGKHRPMVADPDDVVEHWPQACAGCGEQIADGDRV
                     GAGDPVAHQVSEIIMRVEVTEHRRMRVRCRCGKCTLAELPAGVAAGALGPAVAAAAAT
                     LTAARVSRRETARLLADPCGLN"
     gene            complement(766..1320)
                     /locus_tag="VF225_05295"
     CDS             complement(766..1320)
                     /locus_tag="VF225_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144705.1"
                     /translation="MSERAFGCVVGGRDAGQRRERPQRGFVVEQAGAEALGLGVAAAA
                     AVGQQVVDALARGGQVGRERGQVAAVAVGLVERGEDAAQFAGELAAQAPGRAAALAQR
                     DEVPEDVCEAQLALGVVDEQLDGVAIGHDHAVGVLAEQGLCAVAVAALGDLKQRGLLG
                     GRDRQPACLSRALPAGLVSAPNGA"
     gene            complement(1673..2347)
                     /locus_tag="VF225_05300"
     CDS             complement(1673..2347)
                     /locus_tag="VF225_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144706.1"
                     /translation="MEKRLLSRLIDHRVLQHPDALSDERLMDVLLQRRFISMIFTPVY
                     DMGIDALSTQAALDVAREIVREEYPAGKPSHREDLIRDLVALGATKHQILACRPTPST
                     TSMLVETLELMGEAAAGGDEVRVLGMLRFWGEVVVAVEYGQFWKALAPHFSTEERPSR
                     FYQTHHRHDGCEPLATASAKTHSGRLGVCLRELLITPNTADAVSQLEARIVESRLRFY
                     DQFLDS"
     gene            complement(2386..2745)
                     /locus_tag="VF225_05305"
     CDS             complement(2386..2745)
                     /locus_tag="VF225_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144707.1"
                     /translation="MVEGLDALYEENRIRLGVRHNLAILATLAHALDDDARQRLRVAL
                     YDSLPSLAIYRTDDRALVSVFLHGQLAVDAPQFEISGRDTLLGDKIFGEFGTLWSIAQ
                     EIKDLRNWDDNDGGDAA"
BASE COUNT          514 a         1018 c          989 g          473 t
ORIGIN      
        1 gacgtaaccg ttcagggggt cgtcgccgcg gtttgaagga ttcctttgtt ttgcagggaa
       61 tcttgcggtt atgcgggatg aggacgtcga tcttgtctat cgcggagggc gtgacgcggt
      121 cgctgcggtg atcgcggcgc aggcggcacg gatcgacgag ctggtcgcgc agaaccagac
      181 gctcgcggcg cgcgtggccg agcttgagcg tctcgccggc cgcagctcac gcaacagctc
      241 gctgccgcca tccaaggact cgccgcaggc gcgcagggaa cgcccgcgca aacagacgca
      301 gcgcaagcac ggtggccagc agggtcatcc gggcaagcac cgtccgatgg tcgccgaccc
      361 cgacgatgtc gtcgagcact ggccgcaggc gtgcgcgggc tgcggcgagc agatcgccga
      421 cggcgaccgc gtcggcgcgg gcgatccggt cgctcatcag gtcagcgaga tcatcatgcg
      481 cgtcgaggtc accgagcacc gccggatgcg ggtgcgctgc cgctgcggca agtgcaccct
      541 ggccgagctg cccgccggcg tcgcggccgg cgcgttgggg ccggcggtcg ccgctgccgc
      601 ggcgacgctg accgccgccc gcgtctcgcg gcgtgagacg gccaggctgc tggcagaccc
      661 gtgcggacta aactaaagct cagcgcggcg tcggtcgagg cgctcgtcaa gcaggccggc
      721 gacgcactgg aggacaccta tcttgagatt ctcggcgccg tcgactcaag ccccgttcgg
      781 ggcgctgaca agaccagctg gcagcgcgcg cgacagacat gctggctgtc ggtcgcgacc
      841 gccgagcagg ccgcgctgtt tcagatcgcc gagcgccgcg accgcgacag cgcaaaggcc
      901 ctgctcggcg agtaccccga cggcgtgatc gtgtccgatc gctacgccgt ctagctgttc
      961 atcgacgacg ccaagcgcca gctgtgcctc gcacacgtcc tcagggactt catcgcgctg
     1021 ggcgagcgcg gcggcgcgcc cgggcgcctg ggccgcaagc tcacccgcga actgcgcagc
     1081 gtcttcgcca cgctcaacca gcccgaccgc gaccgcggcg acctgaccgc gctcacggcc
     1141 gacctgaccg ccccgcgcga gcgcatccac gacctgctga cccacggcag ccgcagccgc
     1201 gaccccaagg ccaagcgctt ctgcgccggc ctgctcgacc acgaaaccgc gctgtggacg
     1261 ttcacgacgc tgcccggcgt cccggccacc aacaacgcat ccgaacgcgc gctcagacac
     1321 gccgtcctgc ggagaaagac cagctatggc acccagaccg accacgacga ccgcctcgtc
     1381 gaacggctcc tcaccctccg cgaaacctgc cgcctacaac accggcgcgt ccacgaatac
     1441 ctgaccgccg cgatcaccgc cgacctccac cgccagccga tccccgcgct tctcaccccg
     1501 acccgaccgg ccaaccacct aaacggctac tgttcgacat cccggccgac cgatcgtgga
     1561 gcgtaggtac gtgaggtgtg gcgtatgggc gcgcacctgt gtacaggtcg ggcttggaac
     1621 cgggcgggtt gacgcgtgcc caggacgtga gcccccgcct agtctcgggt aatcagctat
     1681 cgaggaactg gtcgtagaag cgtaggcgcg attcgacgat ccgtgcttcg agctgcgaga
     1741 ccgcgtcggc agtgttcggg gtaatcaaca gctctcgcag gcacacgcct agcctgccgg
     1801 agtgagtctt cgctgatgcg gtcgcgagag gctcgcatcc gtcgtgcctg tgatgcgtct
     1861 ggtagaagcg cgacggccgt tcctcagtcg agaagtgagg tgcgagtgct ttccagaact
     1921 gcccgtactc gactgccacg accacctcac cccagaaccg gagcatgccg agaacgcgca
     1981 cctcgtcgcc gccggccgca gcctcgccca tgagctcaag cgtctcgaca agcattgacg
     2041 tggttgacgg agttggtctg cacgcgagta tctgatgctt ggtggcgcca agggcgacca
     2101 agtctctgat gagatcctcg cggtgcgacg gcttaccggc agggtactct tcccgcacga
     2161 tctcacgggc gacgtcgagg gcggcttgcg tcgagagcgc gtcgatcccc atgtcataca
     2221 cgggcgtaaa gatcatcgag atgaaccgtc gctgcagaag cacatccatg agccgctcgt
     2281 ccgatagggc atcggggtgt tgcaggaccc gatgatcgat caggcgcgac agcagccgct
     2341 tctccatgcg gtcagcctcg agaatagggg ctagcgcagc tgtcgtcatg ctgcgtcgcc
     2401 cccatcgttg tcgtcccaat tgcggagatc cttgatctcc tgcgcaatgc tccacagcgt
     2461 gccgaactcg ccgaagatct tgtcgcccag cagcgtgtca cggccgctga tctcgaactg
     2521 cggtgcgtcg acggcgagct gtccatgcaa gaacacgctg accagcgcgc ggtcgtcagt
     2581 tcggtagatc gcaagtgacg gcagcgagtc gtatagcgcg acgcggagac gctggcgcgc
     2641 gtcgtcatcg agcgcatggg cgagcgtcgc gaggatcgcg aggttgtgac ggactccgag
     2701 cctgatgcga ttctcctcgt agagcgcgtc tagtccttcc accatggacg cgcttctcaa
     2761 ggccgcggcg ctcgagtcgt aatgcagcag caagatatcg acgcgagccc cattcatcag
     2821 cgcctgccgc agtgcgcgct cgtagacgct cagctcggga atataggtgt tgaggatcgc
     2881 gatagacccg gcgttccgga tggcatcagt acgcgttcag gttccccgtg agtcggagtg
     2941 ttacgcggtg agcggcgtgg ggatcggcgc tccggtgagg cgggcgttgt ggac
//
LOCUS       DASVEU010000132         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1446561,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000132 DASVEU010000000
VERSION     DASVEU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446561"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VF225_05310"
     CDS             <1..1031
                     /locus_tag="VF225_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144708.1"
                     /translation="IDDLVRALQRIPESQREALVLRELEGRSYKEIAPILGVSQSALE
                     TLLFRARRSLAEELENLVTCDRAELALSQQTDGRLGRKERKRLVAHLDECPSCARVAA
                     VGLKRRRAFKGLAILPLPLSLTLFKGAPSASAAVGLSAIGGGAAVGGGAVGGGVAAKI
                     AVAVVAVTVAGGAGYEGVQQTREPATPAKAVVAKVAPANTSHAKAVAAKVARVKQLPG
                     KVAAKKADHAQAKVAKAAAKADTATKRARTPLPTGRPASSRSETAKLPGNNGRDESAP
                     GLKKALGKVKRPLEGEAEPGKSGIAKLRPQPTAKPLREQPVRAKVAKPKPKPRRLVPL
                     DRGRAPKN"
     gene            1102..1746
                     /locus_tag="VF225_05315"
     CDS             1102..1746
                     /locus_tag="VF225_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144709.1"
                     /translation="MRQEPERWALYTGVVAVVLWIVGIFVVESGGIPGENATDADYLA
                     YYRENDSSILIGSWIFMVGCLAFLVFAVVLWGRLAAAEGGSRLLSNVALIGAAATGVL
                     AMLSAGPDVAAAIQEDDLSAAAAGSMQVLGDAFFVGAELSAILLMLGAGMVALRTGIF
                     PKAWAWFSFFLAVVLVIGPIGWAALIFGLPIWVLGTTFFLSRRSAIAEPTAPPA"
     gene            2159..2332
                     /locus_tag="VF225_05320"
     CDS             2159..2332
                     /locus_tag="VF225_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144710.1"
                     /translation="MLAHEPEVARLLHREHTERLTRAARRAATAAPAPAAHEQAPRKR
                     RVGLAHLHLRARV"
     gene            complement(2400..>2697)
                     /locus_tag="VF225_05325"
     CDS             complement(2400..>2697)
                     /locus_tag="VF225_05325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7144711.1"
                     /translation="TCADVDRAVLGYFEANDLLPLWRQHTGHAIGLRNHEAPFLDVGD
                     HTLIEPGMVFTVEPGLYSDTVGGFRHSDTVVVTPDGIDVLTDYPKDIESLTIPV"
BASE COUNT          421 a          908 c          950 g          418 t
ORIGIN      
        1 cgatcgatga tctcgtccgc gcgctccagc ggatacccga gtcgcagcgc gaggcgctcg
       61 tcctccggga gctggagggc cgctcgtaca aggagatcgc gccgatcctc ggcgtctccc
      121 agagcgcgct cgagacgctg ctcttccgcg cccgccgctc gctcgccgag gagctcgaga
      181 atctcgtcac gtgcgaccgc gccgagctcg cgctgtcgca gcagaccgac ggccggctgg
      241 gccggaagga gcgcaagcgg ctcgttgcgc atctggacga gtgcccgagc tgtgcgcgcg
      301 tcgccgctgt ggggctgaag cgccgtcgcg cgttcaaggg gctcgcgatc ctgccgttgc
      361 cgctctcgct gacgctcttc aagggcgcgc cgagcgcgtc cgcggcggtg gggctctccg
      421 cgatcggcgg cggcgcggcg gtcggcggtg gcgcggtcgg gggtggcgtc gcggcgaaga
      481 tcgccgtcgc ggtcgtcgcg gtgacggtcg ccggcggcgc gggctacgag ggcgtccaac
      541 agaccaggga gccggcgacc ccggcgaagg ccgttgttgc gaaggtcgct cccgccaaca
      601 cttcgcatgc gaaagccgtc gcggcgaagg tagctcgggt caagcagctt ccggggaagg
      661 tcgccgcgaa gaaggcggat cacgcccagg cgaaggtcgc gaaagccgcg gcgaaggcgg
      721 acacggcgac gaagcgcgcg cgcactcctc tgcctaccgg tcgtcccgcg agctcgcgct
      781 cggagaccgc gaagctgccc gggaacaacg gccgcgacga gtccgctccg ggactgaaga
      841 aggcactcgg caaggtgaag cgacccctcg aaggcgaggc ggaacccggc aagagcggaa
      901 tcgcgaagtt gcgtccgcag cccaccgcga agccgctccg cgagcagcca gttcgagcga
      961 aggtcgcgaa gccgaagccg aaaccgcgcc ggctcgtccc gctcgatcgc ggaagagcgc
     1021 ccaagaacta gcgcttccgt cccacttgat cccgtccggc acgcgcgcga ggatgcgccg
     1081 caactcgagc aaggaggggt catgcggcaa gagccggagc gctgggcgct ctacacaggc
     1141 gtggttgcgg tcgttctctg gatcgtcggg atcttcgtgg tcgagtccgg gggcattccg
     1201 ggcgagaacg cgaccgacgc ggactacctc gcctactacc gggagaacga cagtagcatc
     1261 ctgatcggaa gctggatctt catggtcggc tgcctcgcgt tcctcgtgtt cgcggtcgtt
     1321 ctgtggggac gcctcgctgc ggcggagggc ggctcgcggc tgctctcgaa cgtggcgctg
     1381 atcggagctg ccgcgacggg agtgttggcg atgctgagcg ccggcccgga tgtcgccgcg
     1441 gcgatccagg aggacgacct gagcgccgca gcggctgggt cgatgcaggt gctcggtgac
     1501 gccttcttcg tcggcgcaga gctgagcgcg atcctgctga tgctcggggc gggcatggtc
     1561 gcgctccgca ccgggatctt tccgaaggcc tgggcctggt tcagcttctt cctcgcagtc
     1621 gtcctcgtga tcggcccgat cggctgggct gcgctgatct tcggcctccc gatctgggtg
     1681 ctcggaacga cgttcttcct ctcgcgccga agcgcgatcg cggagcccac ggcgccaccc
     1741 gcgtagttgg gatcgagaga gcgggaggcg acgaccgcct cccgctcgcc tctcgaagcg
     1801 atgtcttgac aacgcgggag cgagtcggca tcatggttga cttctaccgg ccgaattaac
     1861 ggcctacccg atcgaaagga ggtcagattg tcccgaaaca tcttctgcgt ctggcctccg
     1921 ctcacggcct agacagcgcc gtgatcgggg ccggacgtcg gctccctcga tagccgtagc
     1981 tcatcgctca gcctgcctga agcgcttgat catgcgaatg ccttgcggta gttgcgacgg
     2041 cagaacacgc gctcaggcgc ccattcaccg atccgtgaag gaaggagaac gcgcaccgtg
     2101 attttcctgc tcgataacga agccaatgcc acatcgaagc catccgaaag gaggaaacat
     2161 gctcgctcac gaacccgaag tcgcccgtct gctgcaccgt gagcacacgg agcgtctcac
     2221 ccgtgccgcc cgccgggctg cgacggctgc acctgccccc gcagcccacg agcaggcgcc
     2281 gcgcaagcgt cgcgtcggcc tcgcccacct gcacctgcga gcccgtgtct agccgccgcg
     2341 gctcaccgcc cgcccggggc tgacggcctt catcgccgct cagctccggg cgtgctcgtt
     2401 cagaccggga tggtcaggct ctcgatgtcc ttcgggtagt cggtgagcac atcgattccg
     2461 tccggagtga cgacgacggt gtcggagtgg cggaagccgc cgacggtgtc cgagtagagg
     2521 cccggctcga ccgtgaacac catgcccggc tcgatcagcg tgtggtcgcc cacgtcgagg
     2581 aacggcgcct cgtggttgcg cagcccgatc gcgtggccgg tgtgctgcct ccagagcggg
     2641 agcagatcgt tggcctcgaa gtaaccgagg acggccctgt cgacgtcggc gcaggtg
//
LOCUS       DASVEU010000133         8861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1253263,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000133 DASVEU010000000
VERSION     DASVEU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253263"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF225_05330"
     CDS             complement(<1..190)
                     /locus_tag="VF225_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7144712.1"
                     /translation="MSEAALNGRRRVVVTGVGMVSPLGNDPETTWQNLVSGESGAGPI
                     THFDTSDYSIRFACELKEF"
     gene            complement(193..924)
                     /gene="fabG"
                     /locus_tag="VF225_05335"
     CDS             complement(193..924)
                     /gene="fabG"
                     /locus_tag="VF225_05335"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7144713.1"
                     /translation="MPETFASLKGKTALVTGGSRGIGRAIALELARAGADVTVSYRSG
                     KDEAEEVAREAGGRAVEADVADPEQAHALIGAVDDLDLLVNNAGVTRDGLIARMSDED
                     WRVVIETNLSGVFHTCRAAARGMMKRRSGSIVNISSIVGLHGNPGQTNYSASKAGIIG
                     FTKALARELGSRNVRANVVVPGYVKTRLTDVLPEEATGAMLENTPLGRLGDPEDVAGA
                     VRFLCSDDASFITGAVLLVDGGLGM"
     gene            complement(917..1849)
                     /gene="fabD"
                     /locus_tag="VF225_05340"
     CDS             complement(917..1849)
                     /gene="fabD"
                     /locus_tag="VF225_05340"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7144714.1"
                     /translation="MKEGLVAFCFPGQGSIEVGMGREIAAAVPEALDVFERSSAASGL
                     DLKHLCFEGPEEKLVQTEIQQPALVATSLAVCAAVRARGIKPDFVVGHSVGEFAALAA
                     AGALKVEEAVALVRERGLAMAETARKNPGSMAAILGLDDEVVERICRRILNVWPANYN
                     CPGQIVVSGENPAVDEACAAAEEAGARRAVKLRVSGAFHSPLVARAADRLRPALEMAK
                     FVEPTSPFMSTVTARIESAKRMAPLLVEQLTAPVRFTHAGQALIKEGVQTFVEVGPGN
                     VLSGLIKRIDRSVKTISVSTPEEVDRLEETLLSA"
     gene            complement(1846..2835)
                     /locus_tag="VF225_05345"
     CDS             complement(1846..2835)
                     /locus_tag="VF225_05345"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7144715.1"
                     /translation="MIGKAQGPPISITGLGAYVPDRVLTNAELSTLVDTSDEWIMERT
                     GIRERRIAAPEQALTDLALPAAQAALAQAGVEGKDIDLLLCATVTPDMMFPTSSALLA
                     DTLGARDAAAYDLLAGCTGFMYALAQAYGMLAAGLAERALVVGGDVLSKILDWTDRST
                     LVLFGDGAGAVVLEKVEKPGFLGFELGADGGGGIHLSLPGSGSRSMDEATGNGYVHMN
                     GREVFKFATRVLVSSAEAVLERCEVSVEDVDVYIPHQANVRILDHAVQKLGIPRERMI
                     VNVDRYGNTSSGSIPLALAEAQADGRLKEGDLVLMTGMGAGLTWGSALMEWTR"
     gene            complement(2832..3272)
                     /gene="fabZ"
                     /locus_tag="VF225_05350"
     CDS             complement(2832..3272)
                     /gene="fabZ"
                     /locus_tag="VF225_05350"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7144716.1"
                     /translation="MTVSTPFGREAIEQILPHREPFLLIDEVLELRPGEGAVARKTVR
                     QDEWYLSGHFPDRPVMPGVLIVEALAQTGAVAVLSEEENRGRLALFAGIDDCRFKRIV
                     EPGDVLELRCDLERVRGPIGRGKAEARVDGELACRATLTFAVER"
     gene            complement(3301..3816)
                     /locus_tag="VF225_05355"
     CDS             complement(3301..3816)
                     /locus_tag="VF225_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144717.1"
                     /translation="MKRAKDGDAKALEALCERHVPRVEKLAGHLLRDPEDARDAAQEA
                     LAKLCVKIRQFRGESQFATWLHRLTINACRDVANRQRARACEPLLEDPRAATDADPAW
                     EADRAELRGELNRCLAEIAPEQARVVVLKDALGFSFEEISAVADMPVGTAKCYAHRGR
                     SRLRDKLSDVA"
     gene            3932..4525
                     /locus_tag="VF225_05360"
     CDS             3932..4525
                     /locus_tag="VF225_05360"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7144718.1"
                     /translation="MADSLAPDGVKPLLRGRFGHVYRYAEVVPSTQRMLANDDGEGAV
                     AVAEEQTEGRGRLGRSWEAPEGTSVLVSVLLLPAVQAARLPELSLVAGGAVAEAIAEV
                     TGIEPTIKFPNDVLIGGRKVAGILAESSEGRVVLGIGLNANQTREQLPADAQTEPTSL
                     RLELGEPVNRVQLLAAILLQLERAYDAWLARGTSASG"
     gene            complement(4500..6563)
                     /locus_tag="VF225_05365"
     CDS             complement(4500..6563)
                     /locus_tag="VF225_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEX7144719.1"
                     /translation="MKPLLVATLLFFASPAAAFGGASLTMREVPLHGERTLAASTTHF
                     DMVGLHWRGSGTVQFRTRSVDGRWSGWQRADPEGEDLPNVGTAEARAALGWRVGNPYW
                     AGASDRIQYRLRGHIERLRAYFVRSPDVRIPLRRVSMAGSPPLIDREAWGANEAIRRV
                     PPSYATALQFALVHHTAGTNSYTASQSAAIVRGIEVYHVKGNGWNDIGYNFLVDKYGQ
                     VFEGRYGGVDKPVIGAHAEGFNTGSVGVAVLGTYGSSAPPAVARTALAKLLAWRLDIA
                     HVDPKSTLTWVSGGNARFASGVPVFIRAVSGHRDTGFTTCPGAALYSQLDAIARQAAS
                     DGLPKLYAPSARGTLGGQVRFRARLSEALPWTVTVADATGAVVANGTGTSQDVDWTWD
                     ASTAVPGSYSWTIAAGDNVLPASGTIGAKPVVLAITTPTALPRTITPNGDGQTDFSQI
                     SYTLTAPATVTATLRGPDGQDLSVLFSQARRPGKQSFRFTAAGVADGRYEIVLSASDG
                     RSIVTSVLPILVDRTVRGFAASPLAVSPNGDGVADELTFDFELAQPASVKLEIAQARK
                     RLTSVYSAELAPGVQTVSWNPTGLKDGKYAGVLTAANDVGTVTHTVLFRIDTVAPNLR
                     VLSFRSLRFRVSEPATIRLTLNGKLVSRTVRGGVFSFHAARVRSVRIVAQDVAGNVSR
                     TLKYP"
     gene            6649..7926
                     /locus_tag="VF225_05370"
     CDS             6649..7926
                     /locus_tag="VF225_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX7144720.1"
                     /translation="MKRACLRGFLLVGAFLAFASSASAATPRVLAIHFDADVNPVTQN
                     YLCDNLSRAAKDGYSAAVIVLNTPGGLLQSKDKIVLCELNAKLPVLVWVGPNGSTAGS
                     AGVWIAEAADFLGMAPQTNIGSSTPIDSGGSNIGSDLRRKLINHEAATLRALAQSHGR
                     NVDWADRAVRVATNATAPEALRMNVIDAVAPTLPSLLDQAEGVKTKPRGYTLRLAGAQ
                     IHNVHLGFFTRILNTLIDPNILPLLFLAGLAGIGFEIFHPGVVLPGALGAVALVTALF
                     GFSVLPINWGGLVLMLLGVALLVTDVLVTSHGALTVAGLISLAVGSIMLFSNASGFHT
                     SKPLVISIAVALGAVWAFAISKAVEARHRPVEVGAQLIAGSVGEVRDNGLVFVNGELW
                     QARTAGGEVLRPGERVRVESLDGLTLTVRPEST"
     gene            7945..8709
                     /locus_tag="VF225_05375"
     CDS             7945..8709
                     /locus_tag="VF225_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7144721.1"
                     /translation="MNAALVIVGVLVFLIVLFLFSAIKVAREYERGIIFRLGRLLPEP
                     KGPGLFLLIPVVDRMVKVDLRTITLTVPPQEVITKDNVPVRVNAVAYFRIVAPKDAIV
                     QIENFMVATSQIAQTTLRSVLGQHVLDELLSEREKINAILQGIIDESTAPWGIKVSIV
                     EVKDVEIPASMQRAMARQAEAERERRAKIINAEGEFQAAERLKDAAAVIGEQPVALQL
                     RYLQTLLELGSSQATTIVFPVDLIRPFLEKKAGEPT"
BASE COUNT         1451 a         3054 c         2901 g         1455 t
ORIGIN      
        1 cgaactcctt cagctcgcag gcgaaccgga tcgagtagtc gctcgtgtcg aaatgcgtga
       61 tcgggccggc tccggactcg ccggagacga ggttctgcca cgtcgtctca gggtcgttcc
      121 ccagcggaga caccatcccg accccggtga ccacgacgcg acggcgtccg ttcaaggctg
      181 cttcgctcat cgtcacattc cgagaccgcc gtccaccagg agcacagcgc ccgtgatgaa
      241 cgacgcgtcg tcggagcaga gaaaccgtac cgctccggcg acgtcctccg ggtcgccgag
      301 gcggccgaga ggcgtgttct cgagcatcgc gccggtcgcc tcctcgggca ggacgtccgt
      361 gagccgcgtc ttcacgtagc ccggcacgac cacgttcgcc cgcacgttgc ggctcccgag
      421 ctcgcgcgcg agcgccttcg tgaagccgat gatcccggcc tttgaggccg agtagtttgt
      481 ctggccgggg ttcccgtgca agccgacgat gctggagatg ttcacgatcg acccggagcg
      541 ccgcttcatc atcccgcgcg ccgccgcacg gcaggtgtgg aagaccccac tgaggttcgt
      601 ctcgatgacg acgcgccagt cctcgtccga catccgtgcg atcagcccgt cgcgggtcac
      661 acccgcgttg ttgacgagca ggtccaaatc gtcgacggcg ccgatcaggg cgtgagcctg
      721 ttcggggtcg gcgacgtccg cctcgaccgc gcgtcctccc gcctcccgcg ccacctcttc
      781 cgcctcgtcc ttgcccgagc ggtagctgac cgtgacgtcc gcaccggcac gtgcgagctc
      841 gagcgcgatc gcccgcccga taccccgcga accgcccgtg acgagggcgg tcttgccctt
      901 cagcgacgca aacgtctcag gcagagagga gtgtctcctc gagcctgtcg acctcttccg
      961 gggtcgagac cgagatcgtc ttcacgctgc ggtcgatgcg cttgatgagc cccgagagca
     1021 cgttgccggg cccgacctcg acgaaggtct gcacgccctc cttgatcagg gcctgcccgg
     1081 cgtgtgtgaa gcgcacggga gccgtgagtt gctccacgag cagcggggcc atcctcttcg
     1141 ccgactcgat gcgtgcggtg actgtggaca tgaagggcga ggtcggttcg acgaacttcg
     1201 ccatttcgag ggccgggcga agacgatctg ccgcgcgggc gaccagtggg ctgtggaacg
     1261 cgcccgagac gcgcagcttg acggcacggc gtgcgcccgc ctcttcggca gccgcgcagg
     1321 cctcgtcgac ggccggattc tcaccggaga cgacgatctg tccggggcag ttgtagttcg
     1381 caggccacac gttgaggatg cgccggcaga tgcgttccac gacctcgtcg tcgagaccaa
     1441 ggatcgccgc cattgaaccg gggttcttgc gagcggtctc tgccatcgct agcccgcgtt
     1501 cgcgcacgag cgcaacggcc tcttcgacct tgagcgcgcc ggccgccgcc agcgcggcga
     1561 attcaccgac ggaatggccc acgacgaagt ccggcttgat gccgcgcgcc cgcacggccg
     1621 cgcacacggc gaggctcgtc gcaacgagag ccggctgctg gatctccgtc tggacgagct
     1681 tctcttccgg cccctcgaag cagagatgct tcaggtcgag cccggaggcg gcgctgctcc
     1741 gctcgaagac gtcgagcgct tcgggaaccg ccgccgcgat ctcgcggccc atcccgacct
     1801 cgatcgatcc ctgtccggga aagcagaatg cgactagacc ctccttcatc gagtccactc
     1861 catcagcgcc gagccccagg tgagccctgc tcccatgccg gtcatcaaca cgaggtctcc
     1921 ttccttcagc cgaccatccg cctgcgcctc cgccagtgcc agcgggatcg agccggaaga
     1981 cgtattgccg tagcggtcaa cgttaacgat catgcgctcc cgggggatac cgagcttctg
     2041 gactgcgtga tccaggatcc gcacgtttgc ctggtgcgga atgtaaacgt cgacgtcctc
     2101 gaccgacact tcgcaccgtt ccaggaccgc ttcggcggac gagacgagca cccgcgttgc
     2161 aaacttgaag acttcgcggc cattcatgtg gacatacccg tttccggtcg cttcatccat
     2221 gctgcgtgag ccgctgccgg gcagagagag gtggatccca ccgccgccgt ccgcccccag
     2281 ctcgaagccg aggaagcccg gcttctcgac cttctcgagc accacagcgc cggcgccgtc
     2341 gccgaagagc acgagcgtcg acctgtcggt ccaatcgagg atcttcgaga ggacgtcgcc
     2401 gccgacgacg agcgcgcgct cggccagccc cgcggcgagc atcccgtagg cctgcgcaag
     2461 cgcatacatg aagcccgtgc agcccgcgag caggtcgtac gccgcagcgt cacgcgcgcc
     2521 gagtgtgtcc gcgaggagcg cactcgaagt cgggaacatc atgtcgggag taacggtggc
     2581 gcagagcagg aggtcgatgt cctttccctc gaccccggcc tgcgccagag cggcctgggc
     2641 ggccggaagc gcgagatcgg tgagcgcctg ctccggcgcg gcgatccgcc gctcgcgaat
     2701 accggtccgc tccatgatcc actcgtccga ggtgtcgacg agcgtcgaga gctcagcgtt
     2761 cgtcagcacc cggtcgggca cgtaagcgcc gagccccgtg atcgagatcg gcgggccttg
     2821 tgctttacca atcacctttc caccgcgaac gtgagagtcg ccctgcacgc aagctcgccg
     2881 tccacccgcg cctccgcctt accgcgccca atcggaccgc ggacacgctc tagatcacag
     2941 cgcagctcga gcacgtcacc cggttcgacg attcgcttga agcggcagtc gtcgatccct
     3001 gcgaagagcg ccagcctgcc tcggttctcc tcttcgctga gaacggcgac cgcacccgtt
     3061 tgcgcgagcg cttcgacgat cagcacgccg ggcatcaccg gccggtccgg aaaatgaccg
     3121 ctgagatacc actcgtcttg acgcaccgtt tttcgcgcga ccgctccctc gcctggccga
     3181 agctcgagca cctcgtcgat gagaaggaag ggctcgcggt gcggcaggat ctgctcgatc
     3241 gcttcgcggc cgaacggcgt ggagacggtc acggaccacc ctctggccgg cgagcgacgg
     3301 tcacgccacg tccgaaagct tgtcgcgcaa ccggctgcgg ccgcggtgcg catagcactt
     3361 cgccgttcca accggcatgt cggcgacggc cgagatctcc tcgaacgaaa atccgagcgc
     3421 gtccttcaga acgacgacac gggcctgctc cggcgcgatc tccgcaaggc agcggttcag
     3481 ctcgccgcgc agctcggcgc gatcggcttc ccaggccgga tccgcgtcgg tcgcggcccg
     3541 cgggtcctcg agcagcggct cgcaggctcg agcccgctgc cggttcgcca cgtctcggca
     3601 cgcgttgatc gtcaaccggt gcaaccaggt tgcgaactgc gactcgcccc gaaactgcct
     3661 gatcttgacg cacagctttg cgagcgcttc ctgcgccgcg tcacgcgcgt cctcgggatc
     3721 gcgaaggagg tggcccgcga gcttctccac gcggggcacg tgcctctcgc agagcgcctc
     3781 gagggccttc gcgtcaccgt ctttcgctct tttcaccagg attggatcgc tcagccgcgc
     3841 atagacaaga agcccttccg gacgggagga ctgcgagatt tgacgccggc ggctgcgtgg
     3901 cagttgcacc tctgcgctct agtgtaggag cgtggcggat tcgctcgccc cggacggcgt
     3961 gaaacccctc ctccgtggcc gcttcggtca tgtctaccgc tatgccgagg tcgtgccttc
     4021 gacccagcgg atgctggcga acgacgacgg cgagggagcc gtcgcggtgg cggaggaaca
     4081 aaccgagggg cggggccggc tcgggcgcag ctgggaggcc cccgagggca cgagcgtgct
     4141 cgtctccgtc ctcctgctcc ccgccgtgca ggcggcgagg ctcccggagc tgtcgctcgt
     4201 cgccggcggc gcggtggcgg aggcgatcgc agaggtgacc gggatcgagc cgacgatcaa
     4261 gttcccgaac gacgtcctga tcggcggtcg caaggtcgcc ggcatcctgg ccgagtcgag
     4321 cgagggccgc gtcgtgctcg gcatcggcct caatgccaac cagacacggg agcaactccc
     4381 tgcggacgcg cagaccgagc cgacgtcact acgcctcgaa ctgggcgaac cggtgaaccg
     4441 cgtccaactt ctcgctgcga ttctgcttca gctcgagcgc gcgtacgacg cgtggttggc
     4501 taggggtact tcagcgtccg gctgacgttc ccggcgacat cctgcgcgac gatgcgcaca
     4561 ctgcgcacgc gtgccgcgtg gaaggagaac acgccgccgc gtacggtgcg actcacgagc
     4621 ttgccgttga gcgtcaacct aatggtcgcc ggctcgctca cacggaagcg gaggctacgg
     4681 aatgagagga cgcggaggtt gggggcaacc gtgtcgatcc ggaacaacac ggtgtgcgtg
     4741 acggtgccga cgtcgttcgc ggccgtgagc acgcctgcgt acttgccgtc tttcagtccg
     4801 gttggattcc acgagacggt ctgaacgccg ggcgcgagtt ccgcggagta gaccgaggtc
     4861 agcctcttcc tcgcctgcgc gatctcgagc ttcacggacg caggctgcgc gagctcgaag
     4921 tcgaacgtca gctcgtccgc aacgccgtcg ccgttcgggg agacagccag cggcgaggct
     4981 gcgaaccccc gaacggttcg atcgaccagg atcggcagca cggaggtcac gatcgaacgg
     5041 ccgtcgcttg cgctcaacac gatctcgtag cgaccgtcgg ccacgcccgc ggcggtgaaa
     5101 cggaacgact gctttcccgg tcgccgcgct tgcgaaaaga ggaccgagag atcttggccg
     5161 tcaggcccgc gcaaggtggc ggtcacggtc gccggggcgg taagggtgta ggagatctgg
     5221 ctgaaatcgg tttgcccgtc gccgttcggc gtgatcgtgc ggggcagcgc ggtcggcgtc
     5281 gtgatcgcga gcacgaccgg cttcgcgccg atcgtcccgc tggcggggag cacgttgtcc
     5341 ccggccgcaa tcgtccagct gtacgacccg gggactgcag ttgaagcgtc ccaggtccag
     5401 tcgacatcct gactcgttcc ggtgccgttc gcaacgaccg cgcccgtcgc atccgcgacg
     5461 gtcacggtcc agggaagcgc ctcgctgagc cgcgcccgga agcgcacctg accgccgagc
     5521 gtgccccggg ccgacggtgc gtacagcttc ggcagaccgt cactcgccgc ctgccgcgcg
     5581 atggcgtcga gctgggagta gagcgcagcg cctggacagg tcgtgaaccc cgtgtcgcga
     5641 tgtccggaca cagcgcggat gaagaccggg acacctgacg cgaagcgcgc gtttccaccc
     5701 gagacccagg tcagggtcga cttgggatcg acgtgcgcga tgtcgagacg ccaggccagc
     5761 agcttggcga gcgcggtgcg cgcaactgcc ggaggagcgc tcgaaccgta cgtcccgagc
     5821 acggcaactc cgaccgagcc ggtgttgaac ccctcggcat gcgcaccgat gaccggcttg
     5881 tcgacaccac cgtaacgacc ctcgaaaacc tgaccgtact tgtcgacgag gaagttgtag
     5941 ccgatgtcgt tccacccatt gcccttcacg tggtagacct cgatgccgcg cacaatcgcg
     6001 gcggactgcg aagcggtgta ggagtttgtg cccgcggtgt ggtggacgag cgcaaactga
     6061 agtgccgtcg cgtaactcgg cgggacgcgg cggatcgcct cgttcgcacc ccacgcctcg
     6121 cggtcgatga ggggcggcga gccggccatc gagacccggc gcagcgggat ccgtacatcg
     6181 gggctgcgga cgaaataggc acgaagtcgc tcgatgtgtc cacgtaaccg atactgaatt
     6241 cgatcggaag cgccggccca gtagggatta ccgacacgcc agccgagcgc ggcgcgtgcc
     6301 tctgccgtac cgacgttggg aaggtcctcg ccctccgggt ccgcgcgctg ccagccgctc
     6361 caccggccgt cgactgaacg cgtacggaac tgcaccgtgc cggagccacg ccagtgcagc
     6421 ccgaccatgt cgaagtgcgt agtcgacgct gcgagggtgc gctcgccgtg cagcggtact
     6481 tcgcgcatgg tcagcgacgc gcccccgaaa gcggctgcag gggaggcaaa gaacagaagg
     6541 gtggcgacga gaaggggttt cattgcgacg gcggccctct gcccagggtg cctgtacacg
     6601 gcttcggcct caagtgggca cgcccttccc tgatagaatc cttacgagtt gaagagggcc
     6661 tgtttgcggg gatttttgct cgtcggggcg ttcctcgcct ttgcttcctc ggcttcggca
     6721 gccacgccac gcgttctggc aatccacttc gacgccgacg tgaaccctgt cacgcagaac
     6781 tacctctgcg acaacctcag ccgggcagcg aaggacggct actccgcagc cgtgatcgtc
     6841 ctgaacacgc ccggcgggct gttgcagtcg aaagacaaga tcgtgctctg cgagctgaat
     6901 gcaaagctgc ctgtgctcgt gtgggtcggt ccgaacggct ccacggccgg ctccgcgggc
     6961 gtctggatcg cggaggcggc cgacttcctg ggcatggcgc cacagacgaa catcgggtcg
     7021 tcgactccga tcgactcagg cggaagcaac atcggctcgg atctccgccg caagctcatc
     7081 aaccacgagg ccgccactct ccgcgcgctc gcgcagagtc atgggcgcaa tgtcgactgg
     7141 gccgaccgtg ccgtgcgcgt ggccacgaac gcaaccgctc ccgaggcgtt acggatgaac
     7201 gtcatcgacg ccgtcgcacc gaccctgcct tccctgctcg accaggcgga aggcgtcaag
     7261 acaaagccgc gcggctacac gcttcgcctc gccggcgcgc agattcacaa cgtgcacctc
     7321 ggcttcttca cacggatcct caacacgctg atcgatccga acatccttcc cctgctcttc
     7381 ctcgccggcc tggccgggat cggcttcgag atctttcatc ccggtgtcgt cctgcccgga
     7441 gcgctcggcg cggtcgcact cgtcaccgcg ctcttcggct tctcggtcct gcccatcaac
     7501 tggggtggcc tggtgttgat gctcctcggc gtcgcgctac tcgtcaccga cgttctcgtc
     7561 acgtcgcacg gcgcgctcac cgtggccggg ctgatcagcc tcgcggtcgg gtcgatcatg
     7621 ctcttttcga atgcgtccgg ctttcatacc tcgaagccgc tggtgatcag catcgctgtc
     7681 gcgctggggg cggtgtgggc cttcgcgatc agcaaggccg tggaggcccg acacagaccc
     7741 gtggaggttg gcgcgcagtt gatcgccggc tccgtgggag aagtgcgcga caacggcctc
     7801 gtcttcgtga acggcgagct gtggcaggca cggactgcgg gtggggaggt acttcgcccg
     7861 ggtgaacgcg tccgcgtcga gtcgctcgac ggcctgactc tcacggtccg gccagagagc
     7921 acctaaaccc cgtaggatca ggtcatgaac gcagcactgg tgatcgtcgg cgtactggtc
     7981 ttcctgatcg tccttttcct cttctctgcg atcaaggtgg cccgcgagta cgagcgcggc
     8041 atcatcttcc gcctcgggcg cctgctcccg gagccgaagg gcccaggtct cttcctgctc
     8101 atcccggtgg tcgaccggat ggtcaaggtc gacctgcgca cgatcaccct gacggtcccg
     8161 ccacaggaag tcatcaccaa ggacaacgtt cccgttcgtg tgaacgccgt cgcgtacttc
     8221 cgcatcgtcg ctccgaaaga cgcgatcgtg cagatcgaga acttcatggt cgcgacctcg
     8281 cagatcgcac agacgacctt gcggtcggtg ctcggacagc acgtgctcga cgagctgctc
     8341 tcggagcgcg agaagatcaa cgcgatcctg caggggatca tcgacgagtc gaccgccccg
     8401 tggggcatca aggtgtccat cgtcgaggtg aaggacgtcg agattccggc ctcgatgcaa
     8461 cgcgcgatgg cacgacaggc cgaggccgag cgcgagcggc gcgccaagat catcaacgcc
     8521 gagggcgagt tccaggcagc cgaacggctg aaggacgcgg cggcggtgat cggcgagcag
     8581 cctgtcgcct tgcagctgcg ctatctgcag acgctcctcg agctcggctc ttctcaggcg
     8641 acgacgatcg tcttcccggt cgatctgatc cggcccttcc tggaaaagaa ggccggcgag
     8701 ccgacctgag cggcgacctc cgccgcgatc cggattcggg acgcgtcgca gtgatcgctc
     8761 ccggcagggc cagccgccca ggcgcagcgc ggccgctact ggaccctccg agcgccggcg
     8821 agctggcgtc gtgcccgttc tgcgccgggc acgaggacat g
//
LOCUS       DASVEU010000134         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_88500,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000134 DASVEU010000000
VERSION     DASVEU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88500"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF225_05380"
     CDS             complement(<1..336)
                     /locus_tag="VF225_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7144722.1"
                     /translation="MATTEDPRARSEESPTAEWKRELYDATPEREGELFSTISGLENE
                     PLYAPDGVEIDYDRDLGYPGVYPFTRGVYPSMYRGKLWTMRQFAGFGTAEETNARFRY
                     LLEHGQTGLS"
     gene            404..1522
                     /locus_tag="VF225_05385"
     CDS             404..1522
                     /locus_tag="VF225_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144723.1"
                     /translation="MEKIVAVVICSLVALTAAGAPPAATNRDLLRSASHLSGLPVRRV
                     VAERTLAGAQYDSAVLRAAQREYPRSLRSVDAALYARLGLMPRSLQAQLASASQASRA
                     WYDPAARRLLLRRTPMPQRARVVNELVRALVDQSFNLRRIAKLRVRDRDRALAAKAIV
                     DGTAALASGVTATPVRGAPLERFLQLESGVGAGKALAKELRYLGGSRALASALRSFPQ
                     TTEQLLHIDKLLEREPAVAVALRPRIGDWKLSASETFGELDVRSLLRTFGVPNPVAAA
                     DGWGGGRMALYLSPTGETTAVLALRWDTIDDAAEWRDAVPRYVEAAFPGATPRDCPPL
                     DHCWSSASTVASGVLGTTSVFASGPAADTVAAALYTEK"
     gene            1851..>3178
                     /locus_tag="VF225_05390"
     CDS             1851..>3178
                     /locus_tag="VF225_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144724.1"
                     /translation="MVSTALLFTAFVAVTELATLRGKKAHDASAFLTRELGPPLDSAP
                     LVRTPAPDVDVSVSPQGFHVKRGPHSLGLALVGGKPQHVTSFAHGISTKTDFGRAVVA
                     VSPKKTEQYLVVDKHHGTRTWSWQLDTAGLTPRVGGDGSIGFIAGHRLTDNFVIAAPK
                     ILDLRGHDISPHGLHWDIGRSEGKWSLELAFDDSKLATPYVIDPASYNVGAGTTGPTA
                     AAASIALALPASIKLGDLLIAHLTWKGGSNITTTQAVPGTWTQLDATRNQSTNIGAQV
                     WYKNATAADTTGGSYTWNFAPNAIATGGIAAYTGIDKSVAPAVSQLVTAATNDNVVYY
                     PAITPAANGAQVISVLGWNRVSNGANLTPALSVNGAAGAPGINTDRWETVNSGAITSE
                     LSDFTQNVAAAVNTNGANTAGGSGKNIAHVAFRISLKADVTAPTSSVALSS"
BASE COUNT          532 a         1101 c         1031 g          514 t
ORIGIN      
        1 cgacaggccg gtctggccgt gctctaggag ataccggaag cgggcgttcg tctcctcggc
       61 agtgccgaag ccggcgaact gtcgcatcgt ccagagcttc ccgcggtaca tggacggata
      121 gacaccgcgc gtgaagggat aaacgcccgg atagccgagg tcacggtcgt aatcgatctc
      181 gacaccgtcg ggcgcgtaga gcggctcgtt ctcgaggccg gagatcgtcg agaagagctc
      241 gccctcacgc tcgggcgtcg cgtcgtagag ctcacgcttc cactcggcgg tcgggctttc
      301 ttcggaccgc gcgcgcggat cctctgttgt cgccatcctt ctttcgattc tacgggcgcg
      361 attccggctc ctgccgtccg gcgctgctgc cgatattcgt cgcgtggaaa agatcgttgc
      421 agttgtcatt tgtagcctgg tggcactcac ggcggcgggt gcgccgccgg ctgcgacgaa
      481 ccgcgacctg ttgcgaagcg cgtcacacct atcggggctg ccggtccgcc gggtcgtggc
      541 ggaacgcacc ctcgccggtg cgcagtacga ctccgcagtc ttgcgtgccg cgcagcggga
      601 gtacccgcgc tcgctgcgca gcgtcgacgc cgctctgtac gcgcggctcg ggctgatgcc
      661 gcgcagcctg caggcacaac tcgcatcggc gagccaggcg tcacgtgcct ggtacgaccc
      721 ggcagcgcgc aggctcttgc tacggcgcac gccgatgcca cagagggcac gggtcgtgaa
      781 cgagttggtg cgggccctcg tcgatcagag cttcaacctg cggcggattg ccaaactgcg
      841 cgtgcgcgac cgtgaccgtg cgctcgccgc gaaggccatc gtggacggga ccgctgcact
      901 ggcgtcgggt gtcactgcaa cgccggtgcg cggcgcgccg ctcgagcgtt tcctgcaact
      961 cgagtccggt gtcggcgccg gcaaagcgct ggcgaaagag ttgcgctacc tcggcggctc
     1021 gcgcgcgctt gcgagcgcgc tgcgatcgtt tccgcagacg accgagcagc tacttcacat
     1081 cgacaagttg ctcgaacgcg agcctgcagt ggcggtagcg cttcgacccc ggatcggcga
     1141 ctggaagctc agcgcgtccg agacgttcgg agagctcgac gttcggagtc tgctgcgcac
     1201 cttcggggtc ccgaacccgg tcgccgcggc cgacggctgg ggcggcggcc gcatggcgct
     1261 ctatctctcg ccaacgggag agacgaccgc tgtgctcgct ctgcgctggg acacgatcga
     1321 cgatgcggcg gagtggcgcg atgcagtgcc gcgctacgtc gaagccgcgt ttcccggcgc
     1381 gacgccgcgc gattgcccgc ccctcgacca ctgctggtcg agcgcgtcga cggtcgcctc
     1441 cggggtgctg ggaacgacga gcgtcttcgc gagcgggccc gccgccgaca ccgtcgcggc
     1501 ggccctctac accgagaagt aaccctcgac tcgagcctta gggtcggcac tacctcggat
     1561 ctgcccagat ccctgacttc gcggaggacg tggcgcggtt actgcgccga ggaagaaatg
     1621 tgcagatttc cgtaccccag aggggggatt tgcaccgaaa cgatacccgt tagaaccgca
     1681 aaacgcttca ggaagccagg cccgagcgcc gattcctcca aacggagcca cgagtcagca
     1741 atggcgcgtg gatggagaat atggcgagct tcccggacct gcggcctcat cggcgctcgg
     1801 cgaaagccga gtcctcggtc gcgtccaaga ccaggactcg tcttctcctc gtggtctcca
     1861 ccgcgctcct gttcaccgcc tttgtcgccg tcaccgagct cgccactctc cgcggcaaga
     1921 aggcacacga cgcatcggca ttcctgacga gggagctggg tccgccgctg gactccgcgc
     1981 ccctcgtcag gacgccggcg cctgacgtcg acgtctcggt ttcgcctcag gggttccacg
     2041 tcaagcgggg cccacactcg ctcgggctgg cgctcgtcgg cgggaagccg cagcacgtca
     2101 cctcgttcgc ccacgggatc tcgacgaaga cggacttcgg ccgtgccgtt gtcgccgtct
     2161 ccccgaagaa gaccgagcag tacctcgtcg tcgacaagca ccatggaacg aggacgtgga
     2221 gctggcagct cgacaccgcc ggtctcactc cacgagtcgg cggcgacggt tccatcggct
     2281 tcatcgccgg ccatcgtctg acggacaact tcgtgatcgc agctccgaag atcctcgacc
     2341 ttcgcggtca cgacatctcg ccgcacggtc tgcactggga catcggccgt agtgagggca
     2401 agtggtcgct cgagctcgcc ttcgacgaca gcaagctcgc gacgccgtat gtcatcgacc
     2461 cggcgtcgta caacgtcggc gctggcacga ccgggcctac ggctgcggcc gcgagcatcg
     2521 cgctcgccct tcccgccagc atcaagctcg gcgacctgct gatcgcacac ctgacctgga
     2581 agggtgggtc gaacatcacg acgacacaag cggttccggg aacgtggacg cagctcgacg
     2641 cgacgcgcaa tcagtcgact aacatcggcg cgcaggtctg gtacaagaac gcgacggccg
     2701 ccgacaccac cggcggctcc tacacctgga acttcgcccc gaatgcgatc gccaccggcg
     2761 gcatcgccgc ctacaccgga atcgacaagt cggttgctcc cgcggtctcg cagctcgtga
     2821 ccgctgcgac caacgacaac gtcgtctatt accccgcgat cacgcccgct gcgaacggcg
     2881 ctcaggtcat ctcggtcctc ggctggaaca gggtctcgaa cggagccaac ctcacgccgg
     2941 ccctctcggt gaacggcgcc gccggcgcac cgggcatcaa caccgaccgc tgggagaccg
     3001 tcaacagcgg ggcgatcacg tcggagctct ccgacttcac gcagaacgtg gctgcggccg
     3061 tcaacacgaa cggggcgaac actgcaggag gatcggggaa gaacatcgcc catgtggcct
     3121 tccggatttc attgaaggca gatgtcaccg caccaacgag cagcgtcgcg ctcagctc
//
LOCUS       DASVEU010000135         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_672025,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000135 DASVEU010000000
VERSION     DASVEU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672025"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /gene="pheT"
                     /locus_tag="VF225_05395"
     CDS             <1..2055
                     /gene="pheT"
                     /locus_tag="VF225_05395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7144725.1"
                     /translation="DARQIVCGAWNFGAGATVGVALPGAVLPNGLRLERRKVRGEVSD
                     GMILAEDEVALGTDHTGIMVLENGIEPGTPLADVLPLTETVLDLGPEHNRPDLLAVYG
                     IAREVAALFDLELAAAPGTDPPPDANETLDIAIEDFEACPRYVGRLFRGATISPSPVW
                     LKARLLAAGMRPISNVVDITNYVMLALGNPLHAFDYTKLAGGRIVVRRAEPGEKLRTL
                     DGVERELEPYDLMIADGERSVALAGIMGGEETEIGANTTEILLEAANFEPYTIFRTSE
                     RLRLRTEGSNRWEKGVDPYLAEQAAKLATELIVEHAGARWVGHADVHAELPAPPVVRF
                     RPERADAVVGIETPPDEQQAILRRLEFGVDGENVSVPTWRARDVTREIDVVEEIARFR
                     LADVPGRLPTRQAMFGRLSKLQRIRRRIEDVLVGFGYSEAYTWSLLPEESATGGVPLQ
                     EPLSSEQAVLRTSLIEGLLASAQRNVDAGNEDIALFEQAHVYLPTGDQLPEERWRVGG
                     IAQGGFAFAKGTAEGLYAALGVEPSFRPADDLPAAGRGARTDEGWVASVRKPDLPGEW
                     GAFELDVDALVDRVPDLIVYEDVITYPPVRQDLAFVVPDEVTAGELVASAHEAAGAEL
                     REMRAFDVYRGGQVEPGKKSIAFSVAFQSPERTLTDEDAGELRNRIVAALEQRFDAQL
                     RA"
     gene            2161..3126
                     /locus_tag="VF225_05400"
     CDS             2161..3126
                     /locus_tag="VF225_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144726.1"
                     /translation="MTKRTAALTSMILLALGIAAFTGSALAGNGNGNGQADTQSVSAD
                     ESASAVTPGNSADEHGNSASAPGQIKQDSVSTQASVSTQASGGGSADQNAGAEAGAKP
                     TSATAKGNKPTMCTTGGGTGSSATCTSSGATAATAQTAAKPDSSKRYGNSETAAQIAN
                     SRGAPAGTEVYGPGNSQPHKVLDCKRNHWVDVHGVKSYSTTSCSQSGTTGGTTTGTTT
                     GTTGSTTVGASASGVTNSTSVTTTPSSNPGATTPGNSTAGGGVLGVTASGGKGTPEGG
                     VLGAIASVGSGVLPFTGFSLWIVALAAAALMAFGLVLRRAGRVIV"
     gene            3273..3908
                     /locus_tag="VF225_05405"
     CDS             3273..3908
                     /locus_tag="VF225_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144727.1"
                     /translation="MATRIVLAALLAALFLPSALARADNPRLIARVGQGDSFTISLRD
                     ASGNPVTHLDPGTYDVEVHDFSEEHNFLLHGPGVDKSTAVGDVEDVVWTVTLADGKYV
                     FECVAHPTTMNGWFTVGQVAPTTISAAVGPKRSISLKPKTAVVGPATITVSDRSRSDN
                     FHLTGLGVNRKTGVAFRGRASWSVTLLPGVYTYRSDKHKTLRGSLVVTLPF"
     gene            complement(3892..4830)
                     /locus_tag="VF225_05410"
     CDS             complement(3892..4830)
                     /locus_tag="VF225_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7144728.1"
                     /translation="MSTATDRDATAEHVPSSPEKLSPAEWLAAFKRSFAAFMKDDCMG
                     LSQQIAYSSLLAFFPAVAFLVGALGLFHLFDDVKTLLDPIAPNGVIKFITSLQKDSQG
                     GTSAAAFLIGFFGAVWAASGAITSVIKAVNRAYERQETRPFWKVRGIAIVLVVTSGIT
                     TAGIFLLIVVGGALGDAIAKKAGLGGAFQWIWGIARWPVAFCAILLFFGLVYYLAPNR
                     EQRSWKWITPGSVVGGLLWLLLSGLFALYVTYAGNYTKTYGTIASGVILLLWLNYSAF
                     ALLFGAELNAELDRQADVRAAGGERAGLVKLARREA"
     gene            complement(4814..>5005)
                     /locus_tag="VF225_05415"
     CDS             complement(4814..>5005)
                     /locus_tag="VF225_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144729.1"
                     /translation="EIGEATNIGAKVRSNLPAVAVGALGVGFLLAGGVGATARLIFRR
                     PREGETKAKLGRFRFVDRD"
BASE COUNT          859 a         1683 c         1683 g          780 t
ORIGIN      
        1 gacgcgcgcc agatcgtctg cggcgcgtgg aacttcggcg ccggcgcgac ggtcggcgtc
       61 gcgctgcccg gcgccgttct gccgaatggc ctgcggctcg aacggcgcaa ggtccgaggt
      121 gaggtcagcg acggcatgat cctggccgag gacgaggtcg cgctaggaac ggaccacacg
      181 ggaatcatgg tcctcgagaa cggcatcgag cccggcacgc cgctcgccga tgtccttcct
      241 ctgacggaga ccgtgctcga cctcgggcca gagcacaacc gccctgacct gctcgccgtc
      301 tatggcatcg ctcgcgaggt cgcagcgctt ttcgacctcg agctcgcagc ggcgcccgga
      361 acggatccac cgcctgacgc gaacgagacg ctcgacatcg cgatcgagga cttcgaggca
      421 tgcccgcgtt acgtgggcag gctcttccgc ggcgcgacga tcagcccttc gccggtctgg
      481 ctgaaggctc ggctgctagc cgctggcatg cgcccgattt caaacgtcgt cgacatcacg
      541 aactatgtga tgcttgcgct cggcaacccg ctgcacgcat tcgactacac gaagcttgcg
      601 ggcggcagga ttgtcgtgag acgcgccgag cctggagaaa agctgcgcac gctcgacggc
      661 gtcgaacgcg agctcgagcc ttacgacctg atgatcgccg atggtgagcg ctcggtcgcg
      721 ctcgcgggga tcatgggcgg agaggagacg gagatcggcg cgaacaccac cgagatcttg
      781 ctcgaggccg cgaacttcga gccctacacg atcttccgca cctcggagcg tctgcgactc
      841 agaaccgagg gctcgaaccg ctgggagaag ggcgtcgacc cgtatctcgc cgaacaagcg
      901 gcgaagcttg cgacagagct catcgtcgag catgcaggag cgcgctgggt tggccacgcc
      961 gatgtccatg ccgagctgcc ggcgccgccg gtcgtgcgct tccgcccgga gcgagccgac
     1021 gccgtggtcg gcatcgaaac ccctcccgac gagcagcaag cgatcttgcg gcggctcgag
     1081 ttcggggtcg acggcgagaa cgtgtcggtt cccacctggc gtgctcgtga cgtcactcgc
     1141 gagatcgacg tcgtcgagga gatcgcgcgc ttccgcctcg ccgatgtgcc cggccgcctg
     1201 ccgacgcgtc aggcgatgtt cggccggttg tccaagctcc agcggatccg ccggcggatc
     1261 gaggacgtcc tagtcggatt cggctactcg gaggcataca cgtggagcct cttgccggag
     1321 gagtcggcga ctggcggtgt cccgctgcag gagcctctct cgagcgagca ggctgttctc
     1381 cgaacgtcgc tcatcgaagg cctcctcgcg tcggcgcagc gcaatgtcga tgccggcaac
     1441 gaggacattg cgctcttcga acaggcgcac gtgtacctcc cgaccggcga ccagctgccc
     1501 gaggagcgct ggcgcgtggg ggggatcgcg caaggcgggt tcgctttcgc caaggggacg
     1561 gccgaaggcc tctatgcggc cctgggggtc gagccgagct tccgtccggc ggacgacctg
     1621 cctgcagccg gccgcggtgc ccgcaccgac gagggctggg tcgcctcagt tcggaagccc
     1681 gacctccccg gcgagtgggg cgcgttcgag ctcgacgtcg atgcactcgt tgaccgcgtc
     1741 cccgatctga tcgtctacga ggacgtcatc acgtatccgc ccgtccggca ggatctcgcc
     1801 ttcgtcgttc cggacgaggt gaccgcgggg gagctcgtgg cgtccgccca cgaagcggcg
     1861 ggggccgagc tccgcgagat gcgtgccttc gacgtctatc gcggtggcca ggtggagccg
     1921 ggaaagaagt cgatcgcctt ctcggtcgcg ttccagtcac ctgaacggac gctcaccgac
     1981 gaggacgccg gagagctccg gaaccgcatc gtcgcggccc tggagcagag gttcgacgcc
     2041 cagcttcggg cgtagctcca gggaaatatt cgttccaacc cgtatccata tgggttgctc
     2101 gctcgttccc ttgccgtaag cctttgtagc tgtgtcattc gaaaacgggg agagaacgag
     2161 atgaccaagc gtactgcggc gctgacaagc atgatcttgc tcgcgctggg catcgccgcc
     2221 ttcacaggct cggctcttgc ggggaacggc aacgggaatg gccaggccga cacccagagc
     2281 gtttccgcgg acgagtccgc ttccgcggtg acgccgggga actcggcgga tgaacacggc
     2341 aactcggcaa gcgcgccggg ccaaatcaag caggactctg tctcgacgca ggcctcggtc
     2401 tcgacgcagg cttcgggcgg cggcagcgcc gaccagaacg ccggcgctga ggccggcgcg
     2461 aagccgacga gcgcaaccgc gaaaggcaac aagcccacga tgtgcacgac gggcggcggc
     2521 accggttcga gcgcgacctg tacctcgagc ggggccactg ccgctaccgc ccagacggcg
     2581 gccaagcccg actcgtccaa gcggtacggc aacagcgaaa ccgcagcgca gatcgcgaac
     2641 tcgcgtggcg cgcctgccgg caccgaggtc tacggtcccg gcaacagcca gccgcacaag
     2701 gtcttggact gcaagcggaa ccactgggtc gacgtccatg gcgtgaagag ctactcgacg
     2761 acctcctgct cgcaaagtgg gacgacgggt ggcactacga ccggcacgac gaccggcacg
     2821 accgggagca cgacagttgg ggcgagcgct tccggcgtca ccaacagcac atccgtcacg
     2881 accacaccgt cgtcgaatcc gggcgcgacc acgcccggca actcgacggc gggcggcggt
     2941 gttctcggcg tgacggcgtc aggcgggaag ggtacgcctg agggcggcgt cctcggcgcg
     3001 atcgcgtcag tgggttcggg cgtactgccc ttcacgggct tctcgctctg gatcgtcgcg
     3061 ctcgccgcgg cggcgctgat ggccttcggc ctggtgcttc gtcgcgccgg ccgggtgatc
     3121 gtctagccga agtaccacca gttcagcagg aaagggcggc cgcgaggccg tcctttctgc
     3181 tttgcagatg cgtggacaag tctccccaga cgacgccgca acgcgcccgc ccacgtaaat
     3241 ccgaaaggcg cgcggcgccg tagagaccgg gcatggccac acgaatcgtg ctcgccgccc
     3301 tactcgccgc gctctttctc ccctcggcgc tcgctcgggc cgacaacccg aggctcatag
     3361 ccagggtggg ccagggcgac tccttcacca tctcgctgcg cgacgcgtcc ggaaatcccg
     3421 tcacgcacct cgaccccggg acgtacgacg tcgaggttca tgatttttcg gaggagcaca
     3481 acttcctact acacggaccg ggagtggaca agtcgacggc cgtcggcgac gtcgaagacg
     3541 tcgtgtggac cgtcacgctg gcggacggca agtacgtttt cgagtgcgtc gctcatccga
     3601 ccaccatgaa cgggtggttc acggtcggcc aggttgctcc gacgaccatc agcgcggcag
     3661 tgggtcctaa gcggagcatc tcgctgaagc cgaagaccgc cgtggtcggg cccgcgacga
     3721 tcacggtgag cgaccgtagc cgtagcgaca acttccacct cacgggcctg ggcgtgaacc
     3781 gcaagaccgg cgtggccttt cgtgggcgtg cgagttggag cgtcacgctg ctgcccgggg
     3841 tctatacgta ccgctcggac aagcacaaga ctctgcgcgg ctcgctggtc gtcacgcttc
     3901 ccttctagcg agcttcacga gaccggcgcg ctcaccgccg gccgcgcgca catcggcctg
     3961 ccggtcaagc tcggcgttga gctccgcacc gaagagaagc gcgaacgccg agtagttgag
     4021 ccacagcaac aggatgacgc cgctcgcgat cgtcccgtac gtcttggtgt agttcccggc
     4081 gtacgtgacg tagagggcga agaggcccga gaggaggagc cagagcaggc cgccgacgac
     4141 cgagcccggc gtgatccact tccagcttcg ctgctccctg ttcggtgcga ggtagtacac
     4201 gagcccgaag aagagcagga tcgcgcagaa cgcaacgggc cagcgtgcga tcccccagat
     4261 ccactggaag gcgccgccca gtcctgcctt cttcgcgatc gcatcgccga gtgctccgcc
     4321 gacgacgatg agcaggaaga tgccggccgt cgtgatcccg ctcgtgacga cgaggacgat
     4381 tgcgattccg cgcaccttcc agaagggacg tgtctcctgc cgctcgtagg ccctgttcac
     4441 ggccttgatc accgaggtga tcgccccgct tgcggcccag acagccccga agaagccgat
     4501 caggaatgcc gctgccgacg ttcctccctg tgagtccttc tgcaggctcg tgatgaattt
     4561 gatcacgccg ttcggcgcaa tcgggtcgag cagcgtcttc acgtcgtcga acaggtgaaa
     4621 gagaccgagc gccccgacga ggaaggctac ggccgggaag aaggcgagga gcgagctgta
     4681 ggcgatctgc tgggagagtc ccatgcagtc gtccttcatg aaagcggcga agctgcgctt
     4741 gaacgcggcc agccactcgg ccgggctcag cttctccggc gagctcggga cgtgctcggc
     4801 ggtcgcgtct cgatcagtcg cggtcgacaa agcggaagcg gccgagcttc gctttcgtct
     4861 cgccctcccg cgggcgccgg aagatcagcc gtgctgtggc cccgacgccc cctgcgagca
     4921 ggaagcccac tccgagcgcc ccgaccgcta ccgccggaag gttggagcgc accttggcgc
     4981 cgatgttggt cgcctcgccg atctc
//
LOCUS       DASVEU010000136         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_672190,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000136 DASVEU010000000
VERSION     DASVEU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672190"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF225_05420"
     CDS             <1..471
                     /locus_tag="VF225_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7144730.1"
                     /translation="ADRGRAVIAGYSWGGYVTLLELGKHPELWICGVAGVPVGDYEAG
                     YEDLSPLLQAYDRALIGGAPSDLPELMRDRNPINFADAVRAPVFFMIGENDSRCPYRQ
                     AMLYVEKLAARDHPHDLYVFPTGHSPFDVDEKVRQIDKILDFLGRHVPGVRTAA"
     gene            complement(562..2517)
                     /locus_tag="VF225_05425"
     CDS             complement(562..2517)
                     /locus_tag="VF225_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX7144731.1"
                     /translation="MKIVVATDRSETAQRAVAWAAQLAQSFDGELILLNVRPAEEDGA
                     EDLLREDAEAFSNARSIFRVDHDVPGGIVRAAEEENADVLVVGNVGMGGRREFLLGNV
                     PNRISHTARCTVVIVNTSDGVVPEPPTAPVVEGRLLGRAAQIARIISRLGLDVRGTIT
                     SAERAQRVRAALEELGPTFAKLGQVLSTRPDLLSEEFVAELEKLQDNVKPLTEAEVVR
                     VMEQELRVPWEDVFASIDSEPLAAGTIGQVHRATLDDGSRVVIKVQRPRARAEILRDL
                     GLFELFAEKALKREQLRETVDIPALVEHLSDSLRRELDFREEAANVERMREALAPYDR
                     LDVPRLYPELSTSRLLVLEFIDGIPILDAPESAERRDAAQQLLEAFSRQILVDGFFHA
                     DPHPGNLLWSDEKIYLLDLGMVGELGPEVREILILLLLAFARDDPKFLAEAVLMLAGE
                     ERRAELDLGSMERDFADFIERFRIGSLRDIKIGPMLDGILQIASHHGIRLPASLALSG
                     KAFGQMQLAVTKLDPTLDPFRVIGRFLLRNVGQRLRRQADPQQLYYEAEKIKLRILRF
                     VEAVERATGARPGQKLQVDFLGAASIEEAIRGVGRRLALAAGAASGLVGAAATAAAGT
                     AGWIPITFAGVAGIFGAWLALDLARRR"
     gene            2760..>3607
                     /locus_tag="VF225_05430"
     CDS             2760..>3607
                     /locus_tag="VF225_05430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7144732.1"
                     /translation="MAKPLEMTAHTRIGTEVAGFRIESVLGRGGMSVVYVAEQIRLGR
                     KVALKLLTTELAWDEQFRERFVRESHIAAATDHPNIIPIYDAGEADGLLYIAMRFVEG
                     PDLKEILKRGSLGVGRTIFLIEQLASALDAAHARSLVHRDVKPGNILVEESTDHAYLT
                     DFGVAKQTTARGLTSTGHFLGTVEYAAPEQIEGGPVDARTDVYALGCVLYECLTGSPP
                     FAQGTEHAVLHAHLVDPPPSVSRARPELPQAFDGVVATAMAKAAADRFPSCGELAHAA
                     RNAASGT"
BASE COUNT          608 a         1197 c         1182 g          620 t
ORIGIN      
        1 gccgatcggg gacgggccgt tatcgcaggc tactcgtggg gcgggtacgt gacgctcctc
       61 gagctcggca agcatcccga gctgtggatc tgcggtgtcg cgggcgttcc cgttggcgac
      121 tacgaggccg gctacgaaga cctctctccg ctcctccagg catacgaccg cgcgctcatc
      181 ggtggcgcgc cgagcgacct gcccgaactg atgcgtgacc gcaacccgat caacttcgcc
      241 gacgcagtcc gcgcgccagt gttcttcatg atcggcgaga acgacagccg ctgtccctat
      301 cgccaggcga tgctctacgt cgagaagctc gctgcccgcg atcatccgca cgatctgtac
      361 gtgttcccaa cagggcactc gccgttcgat gtggacgaga aggtccggca gatcgacaag
      421 attctcgact tcctcggccg gcacgtgccc ggagttcgca cggcggcctg acgtgagggt
      481 gcgcatcgag cgcctgctcc tcggcggctt gatgtcggcg gtcgcctttc tactcgaccg
      541 acgcttacgc aagctccagc gttagcgtcg ccgtgcgaga tcgagcgcga gccatgcgcc
      601 gaagattccg gccacgccgg cgaacgtgat cgggatccag cccgcggtgc cggcagccgc
      661 ggtcgccgcc gcgccgacaa ggccggatgc agcgcccgcc gccaacgcca aacgacggcc
      721 gacgccgcga atcgcctcct cgatcgaggc ggcgccaaga aagtccactt gcagcttttg
      781 gcccggtcgc gcacccgtag cacgctcgac cgcctcgaca aatctgagga tgcggagctt
      841 gatcttctcg gcttcgtagt agagctgctg tgggtcggcc tgtcttcgga gccgctggcc
      901 gacgttgcga aggagaaatc gtccaatgac acggaatggg tcgagcgtcg ggtcaagctt
      961 cgtcactgcc agctgcatct gcccgaacgc cttgccgctg agcgcgagcg atgccggtag
     1021 gcgaatgccg tggtgactgg cgatctgcag aatcccgtcg agcatgggtc cgatcttgat
     1081 gtcgcgcagc gaaccgattc ggaaccgctc gatgaaatct gcgaaatcgc gttccatcga
     1141 tcccagatcg agctcggccc ggcgctcctc gcccgcgagc atgaggacgg cctcggcgag
     1201 aaacttcggg tcgtcgcgcg cgaaggcgag caaaagcaga atcaggatct cccgcacctc
     1261 gggcccgagc tcgcccacca tgcccaagtc gaggagatag atcttctcgt cggaccagag
     1321 gaggttcccc gggtgcggat ccgcgtggaa gaacccgtcg acgaggatct gccgggagaa
     1381 cgcctcgagt agttgctgcg cagcatcgcg gcgttcggcg ctctccggtg cgtccaggat
     1441 cggaatgccg tcgatgaact cgagcacgag caggcgcgac gtcgacaact cggggtagag
     1501 ccgcggcacg tccagtcggt catagggcgc gagcgcctcc cgcatccgtt cgacgttcgc
     1561 cgcctcctcg cggaaatcga gctctcgccg cagcgaatcc gataggtgct cgacgagagc
     1621 cgggatgtcg accgtttcgc ggagctgttc acgcttgagc gccttttccg cgaagagctc
     1681 gaacaagccg aggtcacgca gaatctccgc cctcgctcgc ggccgctgca ccttgatgac
     1741 gacgcgcgac ccgtcatcga gcgttgcgcg atgcacttgc ccgatcgttc ctgcggcgag
     1801 aggctccgaa tcgatcgagg cgaagacgtc ctcccagggg acgcgcagct cttgctccat
     1861 gacgcgcacg acctccgcct ccgtgagggg cttgacgttg tcctgcagct tctccagctc
     1921 ggcgacgaac tcctcagaca gcagatcggg ccgggtcgaa aggacctggc cgagcttcgc
     1981 aaatgtcggc ccgagctctt ccagcgctgc gcgcacgcgt tgtgcacgtt cggccgacgt
     2041 gattgtgccc cggacgtcga gcccgagccg gctgatgatc cgcgcgatct gcgcggcgcg
     2101 tccgaggagc cgcccttcga ccacgggcgc ggtcgggggc tctggtacta cgccgtcgga
     2161 cgtgttgacg atgacgacgg tgcaccgtgc agtgtgcgag attcggttag gcacgtttcc
     2221 gagcagaaac tcgcggcggc cacccatgcc gacgttcccg acgacgagca cgtcggcgtt
     2281 ctcctcctcg gcggcgcgca cgatgccgcc cgggacgtcg tggtcgacgc ggaagatcga
     2341 gcgcgcgttc gagaatgcct cggcgtcctc ccgcagcaga tcttcagctc cgtcctcctc
     2401 tgcgggacgc acgttgagga ggatgagttc gccgtcgaaa ctctgggcga gctgcgccgc
     2461 ccaggcaact gcgcgctggg ccgtttcgga ccggtcggtg gcgacgacga tcttcacgtg
     2521 aggcaaggcc tatttcaaca gacggcgagc cgcagggagg cctgggctgc gcgggcggat
     2581 ctcgccttac tcgggtcatg ctgatcgggg ggatttgcaa gtacttccgc ctctcgacgg
     2641 gctgcgcctc gaggcggcat agtggaagac gtcccgcctg gtctgcgtcc tgcgggcatg
     2701 gaggctcggc cggaagtcct gttgcttggc catgggtttt cctggcgctg gggcagataa
     2761 tggccaaacc cctcgagatg acggcccata cacggattgg aaccgaggtc gccgggttcc
     2821 ggatcgaatc ggtcctcggc cgcggcggca tgagcgtcgt ctacgtcgcc gagcagattc
     2881 gccttggtcg aaaggtcgcg ctgaagcttc tcacgacgga gctggcttgg gatgaacagt
     2941 tccgcgagcg attcgtgcgc gaatcgcaca tcgctgccgc cacggaccat ccgaacatca
     3001 tccctatcta tgacgcaggc gaagccgacg ggctgctcta catcgccatg cgtttcgtcg
     3061 aaggccccga tctcaaggaa atcctcaagc gcggcagcct cggcgtcggt cgcacgatct
     3121 tcctcatcga acagctggcg agcgcgctcg atgctgcgca cgctcgctcg ctcgtgcacc
     3181 gagatgtgaa gccggggaac atactcgtcg aggaaagcac cgaccacgct tacctgacgg
     3241 acttcggcgt agcgaaacag acgactgcgc gcggcctcac gtcgaccggg cacttcctgg
     3301 gcacagtcga gtacgcggcg cccgagcaga tcgaaggtgg gcccgtcgac gcccgcaccg
     3361 acgtctacgc gttgggttgc gtgttgtacg agtgtctgac cggatcgccg cccttcgcgc
     3421 aggggacgga acacgcagtg ctgcatgcgc atctcgtcga tcctccgcct tcggtgagtc
     3481 gcgcccgccc ggagttgcct caggcattcg acggcgtcgt agccaccgcg atggcgaaag
     3541 cggccgcgga ccgcttcccg tcgtgcggag agctcgccca cgctgcgcgc aacgctgcga
     3601 gcggaac
//
LOCUS       DASVEU010000137         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1644593,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000137 DASVEU010000000
VERSION     DASVEU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644593"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF225_05435"
     CDS             <1..192
                     /locus_tag="VF225_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144733.1"
                     /translation="EEAYHRMHAQMWHDRLQDEPRYVAALEELRPLLDERREHTSELP
                     ELWEEMTMVRRSQPAGTKW"
     gene            186..671
                     /gene="paaD"
                     /locus_tag="VF225_05440"
     CDS             186..671
                     /gene="paaD"
                     /locus_tag="VF225_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX7144734.1"
                     /translation="MVTEAQVWDALAEIPDPEIPVISLVDLGVVRAVNVEDGQVRVDF
                     TPTFLGCPALEVMRDAMADQIRALGAEPQINVCLDDSWSTDRITPAGREKLRASGFAP
                     PAPREAAAPTLVQLQSNAHRCPYCGSTETRLENIFGPTPCRSLRYCATCRQPFEQFKT
                     I"
     gene            complement(668..1540)
                     /locus_tag="VF225_05445"
     CDS             complement(668..1540)
                     /locus_tag="VF225_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7144735.1"
                     /translation="MSSTASDRPQAETIVLIHGMWMTPLSWEHWVNHYTDRGHRVLAP
                     AWPGLDKEPEELRRDPSPLRGIDITEVVDHYDKIIRGLDSPPIIIGHSFGGLFTQLLL
                     DRGLGAAGVALGTGAPKGVLRLPYSTLRAAWPALRSPTNLKKETPLTPKQFHWCFTNA
                     LSSEDSDSVYRRYYIPGSARPFFQAGLANFNPNAKTKVDYKNPARPPLLLATGTLDRI
                     CPPSVNESNFKQQEKAPSVTEHKEYPGRSHWPGQDGWEEVADYFLNWTTERARSRAAQ
                     PAAAAREEASISQD"
     gene            1737..1883
                     /locus_tag="VF225_05450"
     CDS             1737..1883
                     /locus_tag="VF225_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HEX7144736.1"
                     /translation="MKRGTAHDAEAVSGQKSQFDVVADGKLIFSKQKEGRFPEHEEII
                     RALP"
     gene            complement(1864..2448)
                     /locus_tag="VF225_05455"
     CDS             complement(1864..2448)
                     /locus_tag="VF225_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX7144737.1"
                     /translation="MVDGRVRIEAGESLRRRRAVVLFRAPLVVPHYLVLAAWTLLVVP
                     VLVIAWLALLIEGRLPSFLLRFLGAFLRYQGQVTAWFGLLSARYPDPLHTLEHPFRIE
                     LPERPPQPRSVTLFRLPLALPALVLASVFNVVLSLASVAAWFVALALGRTTAGLQELG
                     TFCLRYQLETQAYVTLLTSAYPRLAPPFVTAAPE"
     gene            2468..2914
                     /locus_tag="VF225_05460"
     CDS             2468..2914
                     /locus_tag="VF225_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144738.1"
                     /translation="MRRAALIALALVFVLSGCGGGGSGRALTKEEYASKADAICGTFN
                     QQTKSLATPKNLSDLAKIADETLPILDRAINDLADLRPPASEKALSDQWLAQVRNLRG
                     DLQEIRDKAKSGDVAAVQAVVPKATDHNSQSNQLATQLGMSVCNRD"
     gene            complement(2911..3681)
                     /locus_tag="VF225_05465"
     CDS             complement(2911..3681)
                     /locus_tag="VF225_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7144739.1"
                     /translation="MAEVETSREGAVLTITLNRPDVLNAFNAALHEGLAAALKEARGP
                     DVRAVVVTGAGRGFCVGQDLTEFREAPGDIGSRLRSTYHPNVLAVRALEKPVIAAVNG
                     VAAGAGMSFACACDLRIAADSASFVPAFINIGLIPDSGGSYFVTRILGPARAFEWLVS
                     GRKLTAAEAHAWGLVSEVVETDALAARAAEVAAQLAALPTRGIGMTKRLIDHALHATL
                     EQQLEREAELQTAATQTEDFKEGVAAFLEKRPPEFRGH"
     gene            3737..>4080
                     /locus_tag="VF225_05470"
     CDS             3737..>4080
                     /locus_tag="VF225_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7144740.1"
                     /translation="MELTFISSAASEVSEEEALDAYSLAVTSVAERLSPSVGNLRVSR
                     RVRGGRILDGGGSGVVITPDGYTLTSAHVVARTEGKGRASFVDGRELQFEVVGSDPLS
                     DLAVLRVDARDLV"
BASE COUNT          714 a         1330 c         1399 g          637 t
ORIGIN      
        1 gaagaggcgt atcaccgcat gcacgcgcag atgtggcacg accgtttaca ggacgagccc
       61 cgctatgtcg ctgcgctgga ggagcttcgg ccgctgctgg acgagcgccg cgagcacacg
      121 tccgagctgc cagagctgtg ggaagagatg acgatggtgc ggcgctccca accggcgggc
      181 acgaaatggt gacggaagct caagtctggg acgcgctcgc cgagattccg gatccggaga
      241 tcccggtgat ctcgctcgtc gacctcggtg tcgtgcgagc agtgaacgtg gaggacgggc
      301 aggttcgcgt cgacttcacg ccgaccttcc tcggctgtcc cgcgctcgag gtcatgcgcg
      361 atgcgatggc ggaccagatt cgcgcgctcg gcgcggagcc gcagatcaac gtctgcctgg
      421 acgactcgtg gtcgaccgac cgcatcacgc ccgcagggcg cgagaagctg cgggcctccg
      481 gcttcgcgcc gcccgcgccg cgtgaggcag ccgcgccaac gctcgtgcag ctgcagagca
      541 atgcgcaccg ctgtccgtac tgcggctcga cggagacacg actcgagaac atcttcggtc
      601 cgaccccctg caggtcgctg cgttactgcg cgacctgcag gcagccgttc gaacagttca
      661 agacgatcta gtcctgcgag attgacgcct cttcccgcgc cgccgcggca ggctgtgccg
      721 ctcgtgatcg cgcccgctcg gtcgtccagt tgaggaagta gtccgcgact tcctcccacc
      781 cgtcctggcc cggccagtgc gaccggccgg gatattcctt gtgctcggtg acagagggag
      841 ctttctcctg ttgcttgaag ttcgactcgt tcaccgaagg cggacagatg cgatccaacg
      901 tcccggtggc caacagcagc ggcggccgcg ccgggttctt gtagtcgacc ttggtcttgg
      961 cattcgggtt gaagttggcc agcccggcct ggaagaaggg ccgcgcgctt cccgggatgt
     1021 agtaacgccg gtaaacggaa tccgagtcct cactgctgag cgcattcgtg aagcaccagt
     1081 ggaactgctt cggcgtcagg ggcgtctcct tcttgagatt cgtcgggctg cgcaatgctg
     1141 gccaggccgc cctgagcgtg gagtacggca gcctcagcac gcccttggga gctcccgtcc
     1201 caagagcaac accggcggcg ccgagcccgc ggtcgagcaa gagctgcgtg aagagacccc
     1261 caaaggaatg gccgatgatg atcggtgggc tgtcgagccc gcggatgatc ttgtcgtaat
     1321 ggtcgacgac ctcggtgatg tcgatcccgc ggagcggcga gggatcgcga cgcagctcct
     1381 ccggttcctt gtcgaggccg ggccacgcgg gcgcgagcac gcggtggcca cgatcggtgt
     1441 agtggttgac ccaatgctcc cagctgagcg gcgtcatcca catgccgtgg atcaggacga
     1501 tcgtctcagc ctgcggtcga tctgaagcgg tagagctcat ggctcctccc cggttgtcgc
     1561 tcgcggaccc agaacccgca ctgcccttcg tcgagttatc gcacagtcag ggaagccagg
     1621 gccagtttcc ggtggcctcc cgttgctccg gtaggctcgt gcaggtgaag gtcgagatct
     1681 tctactgccc cgtctgagac ggctatgacc gccgggccgc gagtctcgcg gccgagttga
     1741 agcgaggcac cgctcacgac gccgaggccg tgtcgggaca gaagagccag ttcgacgtcg
     1801 tcgccgacgg gaagctgatc ttctcgaaac agaaggaagg ccgcttcccg gagcacgagg
     1861 agatcattcg ggcgctgccg tgacgaacgg cggcgcgagc cgaggatagg cggaggtgag
     1921 caacgtcacg tatgcctgcg tctcgagctg gtagcgcaga cagaaagtcc cgagctcctg
     1981 caggcccgct gtagtccggc cgagcgccag ggcaacgaac caggcagcga ccgaggcgag
     2041 cgagagcacg acgttgaaaa cgctcgcgag aacgagcgcg ggcagtgcga gcggcagacg
     2101 aaacagcgtc acagaccgcg gttgcggcgg acgctcgggc aattcgatcc gaaacggatg
     2161 ctcgagcgtg tgcaacggat ccggataccg ggccgagagc aggccgaacc acgcggtgac
     2221 ctggccctgg tagcgcagga acgcaccgag aaagcgaagg aggaaggacg gcagccggcc
     2281 ctcgatcaga agagcgagcc aggcgatcac tagcaccggt acgacgagca gtgtccaggc
     2341 cgcgagcacg aggtaatgcg ggacgacgag cggggctcga aacagtacga ccgcccggcg
     2401 cctgcgcagt gactcaccgg cttcgatccg gacgcgaccg tcaaccacgc tcgataccgt
     2461 agtgccgatg cggcgggcag ccctgatcgc gcttgcgctc gttttcgtgc tctccggctg
     2521 cgggggcggc ggcagcggca gggcgctgac gaaagaggag tacgcgtcga aggccgacgc
     2581 gatctgcggc acgttcaacc agcagacgaa atcgctcgcg accccgaaga acctctccga
     2641 tctggccaag atcgccgacg agacgctgcc gatcctcgat cgcgccatca acgacctcgc
     2701 cgacctgcgt ccgcccgcaa gcgaaaaggc gctctccgat caatggctgg cgcaggtgag
     2761 gaatctcagg ggcgacctgc aggagatccg cgacaaggcg aagtcgggcg acgtggcggc
     2821 cgtgcaggcg gtcgtcccca aggcgacgga tcacaactca cagagcaatc agctggcgac
     2881 ccagctgggc atgtccgtct gtaataggga ttagtgcccg cggaactcgg gcggccgctt
     2941 ctcgaggaac gcggcgacgc cttccttgaa gtcttcggtc tgcgttgcgg ccgtctggag
     3001 ctccgcctcc cgttcgagct gctgctccag ggtcgcgtgt aaggcgtgat cgatcagtcg
     3061 cttcgtcatc ccgatgccgc gcgtcggcag cgcggcgagc tgtgctgcga cctcggcggc
     3121 gcgggccgcc agcgcgtcgg tctcgaccac ctcggagacg aggccccacg cgtgcgcttc
     3181 cgccgccgtg agcttgcggc ctgacacaag ccattcgaac gcccgcgcgg gcccgaggat
     3241 gcgcgtcacg aagtaggagc cccccgaatc cgggatgagc ccgatgttga tgaaggccgg
     3301 cacgaagctc gccgaatctg cggcgattcg caggtcacac gcacatgcga acgacatgcc
     3361 tgcacccgcg gctaccccgt tcacggccgc gatcacaggc ttctcgagtg cacggacagc
     3421 aagcacgttc gggtgatagg tcgagcgcag gcgcgagccg atgtcgcccg gggcctcgcg
     3481 gaactcggtc aggtcctggc caacgcagaa tccgcgcccg gcaccggtga cgacgacggc
     3541 gcggacgtca gggccgcgag cctccttgag cgcggccgca agaccctcgt gcagtgcggc
     3601 attgaacgcg ttgaggacgt ccgggcggtt gagcgtgatc gtgaggacgg cgccctcgcg
     3661 actcgtctcg acctcggcca tcggcgggac tttcgcagat cgacggagct atgggcggcg
     3721 ttgctagctt ggccgaatgg agctcacgtt catctcgtcg gcggccagtg aggtctccga
     3781 ggaggaagcg ctcgacgcct actcgctggc cgtcacgagt gtcgccgagc ggctctcccc
     3841 ctcggtcggc aatctccgcg tgtcgcggcg cgttcgcggc ggtcggatcc tcgacggagg
     3901 cggcagcggc gtcgtgatca cgcccgacgg ctacacgctc acatccgcgc acgtcgtcgc
     3961 gcgcaccgag ggcaaagggc gcgcctcttt cgtcgacggg cgcgagctcc agttcgaggt
     4021 cgtcggctcc gatccgctct cggacctcgc ggtactgcga gtcgacgcac gcgacctcgt
//
LOCUS       DASVEU010000138         6746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_90538,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000138 DASVEU010000000
VERSION     DASVEU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90538"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF225_05475"
     CDS             complement(<1..180)
                     /locus_tag="VF225_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144741.1"
                     /translation="MPRWLARLLAGEAGVTLRTEIRGASNAKAKRELGWTPHYPSWRE
                     GFFTGLGGSAAASVGS"
     gene            322..1137
                     /locus_tag="VF225_05480"
     CDS             322..1137
                     /locus_tag="VF225_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX7144742.1"
                     /translation="MGSIRSASTLAAAAAAIAVLLVTPAGAVIGGTDDTANRFANVGV
                     LQLQIEGDWFDFCSGTLVRTDVVLTAAHCTDFLVEEGPDGFGPDDLRISFDPEGDAPF
                     ATVDHIVVHPDWFTAPPCIGNSKQRCLAPPAEDIALVFLDDPVAGVAPAPIADADYLD
                     ALNLKSETFTVVGYGTDEFITGSVVAPKAIVVVDGVRSYRDVTAIPARDAFPDRFLKI
                     TQSTCFGDSGGPLFHEGTVVGINTWTFSLRCSGPNFAYRVDSTAALTFLEANL"
     gene            complement(1192..2604)
                     /locus_tag="VF225_05485"
     CDS             complement(1192..2604)
                     /locus_tag="VF225_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144743.1"
                     /translation="MAHWLRDRGFEAYAVRGGVAALEGKPPPLSPEDVATAEAAGRAL
                     GALRDPRFRLYSTGVLISLTAKSVEGAAYGYVVLLVGGSAATLGVIGFLSTIPNLVLA
                     LPGGALADRYDQRKLLLAFQGTSMLVPISLAILSATDSLTVPVMGTLALVGGVLGALS
                     FPAFQGMLASMVPKKDLESAVAINSMSLHAARFVGPAVAGLLLATGGPTWIFVVNAAG
                     FPFVLTALLLLPASQTRGGEGVTKLGGAISDGVRYVLGQRSIASLMVLTVFAGVFATP
                     AVAFMLPAIVRFQLDGGGGTLGALTAAMGLGSLLGSLALLIFSRRPNKGGPILVGYLA
                     TALSIAAVGASSSVPLSLALAFVGGFAAVLFIGLSTVVVQLMSSDDMRARAMAIWAAA
                     FVGVLPIGGLVTAALAAWIGPGNAVLVDGLVTLLGGLIVVALRPEIRWLGCAALPEAC
                     IAATNPMAIAMKADVETART"
     gene            complement(2629..2865)
                     /locus_tag="VF225_05490"
     CDS             complement(2629..2865)
                     /locus_tag="VF225_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144744.1"
                     /translation="MAEPTRRYHVWSALRSSSLRLEVYAARELTAGGALLIDVRRQED
                     ASTRIEGARRIPPDEIPSVVAELPRGTPIVLACT"
     gene            3787..4095
                     /locus_tag="VF225_05495"
     CDS             3787..4095
                     /locus_tag="VF225_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144745.1"
                     /translation="MADNFKGQEVDMPVAMLVDNPEGSQEIYEKVRAQLGLETPAGGI
                     FHAAGPSPNGGWRVLEVWESEEDARRFAQERLMPAFDAAGVPRPPQPPQVWQLHNYMK
                     "
     gene            4239..5747
                     /locus_tag="VF225_05500"
     CDS             4239..5747
                     /locus_tag="VF225_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7144746.1"
                     /translation="MGLMFYPRGGSAQVARYLSRALVESGWEVVLAAGSLGSLGEQTH
                     AATFFEGLQVEVADYTPALERFGRGEDQLAGPVPFHGSFEDRERAPDPVFAALGPEQA
                     QAQVSAWEGVFERAGFGHVDLIHLHHLTPMHEAIARLWPERALVTHLHGTDLKMLDRV
                     ERLDAVAVSLDTGLHRLADAVESGEEPRDDHLPETERELLRRTNLSRYRYGRDWASRL
                     EASARRSRRIICISPHEASEAVRLLGVPDDLIEVIPNGVDTDLFDRSALTRDERLKLL
                     RHWLVDDPQGWDESGLPGSIRYPLAALDAFTNHGGEPLPLLLFVGRYLGFKRVPLLVR
                     AYARARPRFKTPVPLLIWGGAPGEWEGEHPYTVARELAVDGVFFSGWRGHDELPQALS
                     CADVFVGPSVDEPFGQVFLEAMSCGLPVITTSTGGPLSFVNTVAGTPNGWLVEPDDVD
                     ALADALVEAVNDDAARKERADHARQQIRGAFSWRALARRFDALYTDVLDRAS"
     gene            complement(5809..6015)
                     /locus_tag="VF225_05505"
     CDS             complement(5809..6015)
                     /locus_tag="VF225_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144747.1"
                     /translation="MGVHLQAAAEVGTGDSHDDLTIQWRALQFARLGFENADEMARSN
                     VDWHEAAAHIAAGATPEHIARILL"
BASE COUNT         1120 a         2216 c         2319 g         1091 t
ORIGIN      
        1 gctgccgacg ctcgccgcgg cggaaccacc cagccccgtg aagaacccct cgcgccacga
       61 cgggtagtgg ggcgtccagc cgagctcgcg cttcgccttc gcgttcgagg cgcccctgat
      121 ctcggtcctg agcgtgacgc cggcctcacc cgccaggagc cgagcgagcc agcgcggcac
      181 ccggcggggc ggcttcgcgc cgatcggcgc cgccagcgcg cttgttctcg accacgcggt
      241 cgggagggcg tccgagaccg gatcttgtat tcgcgttcga cgcgtcgtaa cgttggccac
      301 aaactcgagg aggaggaagc aatggggtcg atacgcagcg cctcgacact tgctgcggca
      361 gccgcagcga ttgccgtctt gctcgtcaca ccggccgggg cggtgatcgg cgggacagac
      421 gacacggcca accggttcgc gaacgtcggc gtgctccagc tgcagataga aggcgattgg
      481 ttcgacttct gctcggggac gctcgtccgc acggacgtcg tcctcaccgc cgcccactgc
      541 accgacttcc tcgtcgagga agggccggac gggttcggcc ccgacgatct gcggatctcg
      601 ttcgatcctg agggagacgc gccgttcgcg acggtggatc acatcgtcgt gcatcccgac
      661 tggttcaccg cgcctccctg tatcggcaac tccaagcagc gttgccttgc gcctccggcc
      721 gaggacatcg cgctcgtgtt cctcgacgac ccggtcgctg gcgtggcgcc cgcgccgatc
      781 gcagacgccg actacctcga cgcgctgaac ctgaagagcg agacgttcac cgtcgtcggc
      841 tacgggaccg acgagttcat tacgggcagc gtcgtcgcac ccaaggccat cgtcgtggtc
      901 gacggcgtgc ggagctatcg ggacgtgaca gccatcccgg cgcgggatgc cttccctgac
      961 cgcttcctga agatcacgca gagcacgtgc ttcggggact cgggcggccc cctgttccac
     1021 gagggcaccg tcgtggggat caacacctgg acgttcagcc tgcgttgttc gggcccgaac
     1081 ttcgcgtacc gcgtcgactc cacggcggcg ctgacgttcc tcgaggcgaa tctctagacc
     1141 tcggagtgcg ccggctggtg gggctccgct acgccagccg gcgcacgatc gtcacgtccg
     1201 tgccgtttcg acatccgcct tcattgcgat cgccatggga ttggtcgcgg ctatgcacgc
     1261 ttccgggaga gcggcgcatc cgagccacct gatctccggc cggagggcga cgactatcaa
     1321 gccgccgagg agcgtcacga ggccgtccac gagaacggcg ttgcccgggc cgatccaggc
     1381 ggccagggcg gccgtcacga ggccgccgat gggaaggacg ccgacgaagg cggcggccca
     1441 gatggccatg gcgcgggcgc gcatgtcgtc ggagctcatc aactggacga ccacggtgga
     1501 gagaccgatg aagaggacgg cggcgaagcc gcccacgaag gcgagggcga gggagagcgg
     1561 gacggaggac gaggcgccga ccgccgcgat cgagagggcc gtggcgaggt agccgacgag
     1621 aatgggcccg cccttgttcg gccgtcgcga gaagatgagg agggcaagcg agccgagcag
     1681 ggatccgagg cccatggcgg ctgtcaaggc gcccagggtg ccgccgccgc cgtcgagctg
     1741 gaagcggacg atggcaggga gcatgaacgc cacggcgggc gtggcgaaca cgccggcgaa
     1801 cactgtcagc accatcaggg acgcgatgct ccgctggccg aggacgtagc gcactccgtc
     1861 cgagatcgca ccgccgagct tcgtcacgcc ctcgcctccg cgagtttgcg aagccggcag
     1921 gaggaggagc gcggtcaata cgaaggggaa cccggcggcg ttcacgacga agatccacgt
     1981 cggcccgccg gtcgccagga ggagaccggc aaccgccggg ccgacgaacc gggcggcgtg
     2041 cagggacatg ctgttgatgg caacggcgct ctcgaggtcc ttcttgggaa ccatggatgc
     2101 gagcatgccc tggaaggctg ggaagctgag tgcgccgagg acgcctccaa ccaacgcgag
     2161 ggtgcccatc accggcaccg tgagcgagtc ggtcgccgag aggatggcga gcgatatcgg
     2221 gacgagcatg ctggtgccct ggaaggcgag gaggagcttg cgctggtcgt accggtcggc
     2281 gagcgctccc ccgggcaggg cgaggacgag gttcgggatc gtgctcagga atccgatcac
     2341 cccgagcgtc gccgccgacc caccgacgag caagacgacg tagccgtagg cagctccctc
     2401 gaccgacttg gcggtaagcg agatcaggac gccggtcgaa tagaggcgaa accgcgggtc
     2461 gcgaagcgcc ccgagggcgc gacccgcagc ttcagccgtc gctacgtctt cgggcgagag
     2521 cggcggaggc ttaccctcga gcgcggcgac accgccgcgg acggcgtatg cctcgaaccc
     2581 tcggtccctc agccagtgcg ccacacggac gctcgtcgcc tcacggagtc aggtacaggc
     2641 gagaacgatc ggcgtaccgc ggggaagctc ggcgacgacg gacgggatct cgtccggtgg
     2701 gatacgacgc gctccctcga tgcgagtgct cgcgtcctcc tggcggcgca catcgatcag
     2761 aagcgcgccc ccggccgtca gctcgcgggc ggcgtagacc tccagccgca gcgacgaaga
     2821 gcggagagcg gaccaaacgt gatagcggcg ggtcggttca gccatcggag ggacctattc
     2881 gatatcacgc cgggccaagg tccgagatgc gcctgggagg attcgaacct ccgacccgcg
     2941 gattagaagc gggtcccgct gggttcgcga tccctgggga ggcctggtga acctgcatgg
     3001 ttaagcggaa tcgcttcgag tcttctcgga cgacctggcg tccccctgtt gacccgccgt
     3061 tgacccagcg cgacgcgggt ggagaggcgg cggattaggg tcacgctcgc cgtccgccgc
     3121 agccttaacc atcgcgatgg cgtgcagaga cggccgatgc ggctcaacgg cgcggttcgt
     3181 ccagaaaaga ggtcgtgcca cttctcgtgt tggggttcgt gataacagga agggtggttc
     3241 ggagcgcggc gccaaccccg gctttgcata ccgaggccag cgatccttcg ggctaagaaa
     3301 gaagatttgc caggaccaag ggagctcatg cggcggtctt cgagaccgca caaaacgtgc
     3361 tgtttgcagg tcttttccct taactcccca atgaattcgc cagtgccaag ctcgagccgt
     3421 gctctagccc tgcccctcag cgagaagacc gtcgacccct tccttaccat ggaggtccag
     3481 gagcgtcacg cgcgtacacg cgcgatcact cacgacgcag tttcccctgc aaatcggact
     3541 gcctgagcgg tagacgaggc gtcgcgaggc gtcgcgcgtg tcgtttctga tgtgtccgtt
     3601 ttgttccgcc gcctggtgcc cagaggtccg gcggactcgg atagggagga gctggcccga
     3661 gagcagccag ggcctgttgc cgctcatcgc caccccgcgc gccaaggctc cagtccagaa
     3721 aggctctatc cgggtcgcgg cgtgcgaggg ggccaaacct gcttgaggga gggctatagg
     3781 ctccccttgg ctgacaactt caagggtcag gaggtcgaca tgccggtcgc aatgctggtg
     3841 gacaaccccg agggttcgca ggagatctac gagaaggttc gggcgcaact cggcctggag
     3901 acacccgcgg gtggcatctt ccacgcagcc gggccgagtc cgaacggcgg ttggcgtgtg
     3961 ctggaagtgt gggagtcaga agaggacgca aggcggtttg cccaggagcg cctcatgccc
     4021 gcattcgacg cagccggggt acctcgcccg ccgcagccgc cgcaggtttg gcaactgcac
     4081 aactacatga agtagctcgc tccgccgtac ggcgcacgca gtacccgccg gcgtcgggga
     4141 tccttccctg ctctgcgagc tttgcgacta cctgttgcgg cagggctggg cgctagcagg
     4201 aggttccgcg gctgaacggg caacgcagac gactgggaat gggtctgatg ttctacccgc
     4261 gcggcggttc ggcccaggtt gcccggtatc tctctcgcgc gctcgtggag tccggctggg
     4321 aggtcgtgct tgccgcaggg tcgctcggct cgctcggcga gcagacgcac gctgccacct
     4381 tcttcgaggg gctccaggtc gaggtagcgg actacacgcc cgctctcgag cgcttcgggc
     4441 gcggcgagga tcagctcgcg gggccggttc ccttccacgg ctccttcgaa gatcgggagc
     4501 gagcgccgga tcctgtcttt gccgctctgg gcccggagca ggcgcaggct caggtgtcgg
     4561 cgtgggaagg cgtgttcgag cgcgcaggct tcggccacgt cgacctcatt cacctccatc
     4621 acctgacacc gatgcacgag gccatcgctc ggctttggcc cgagcgcgcg ctcgtcaccc
     4681 atctgcacgg caccgacctg aagatgctcg atcgagtcga gcgcctcgac gcggtggctg
     4741 tctcgctcga cacgggcctc caccggcttg cggacgcagt cgagtcaggt gaggagccgc
     4801 gcgacgacca cctgcctgaa acggaacgcg agctgctgcg gcggacgaac ctgagccgat
     4861 accgctacgg aagagactgg gcctcgcgcc tcgaagcgtc tgcccgccgc agccggcgca
     4921 tcatctgcat ctcgccgcac gaggcgtccg aggccgtgcg tctcctgggc gtcccggacg
     4981 acctgatcga ggtgatccca aacggcgtcg atacggatct cttcgaccgc tcggcgctca
     5041 cgcgcgacga gcgtctcaaa ctcctgcgcc actggctcgt cgacgacccc cagggctggg
     5101 acgagtcggg gctccccggg agcatccgct acccgttggc ggcgctggat gcgttcacga
     5161 atcacggcgg ggagcccctt ccgctgctcc tcttcgtcgg gcgctacctc ggcttcaagc
     5221 gcgtcccgct cctcgtgcga gcctacgctc gggcgcggcc acggttcaaa acccctgtgc
     5281 cgctcttgat ctggggcgga gcgccggggg agtgggaggg cgagcacccg tacacggtcg
     5341 cgagggagct ggccgtggat ggtgtcttct tcagcggttg gcgcgggcac gacgagctcc
     5401 cgcaggcgct ttcctgtgcc gacgtcttcg tcggcccttc ggtcgacgag cccttcgggc
     5461 aggtgttcct cgaggcgatg tcgtgcggtc tcccggtgat cacgaccagc accggtgggc
     5521 ccctctcctt cgtcaacacc gtcgctggga cgccgaacgg ctggctcgtc gagccagacg
     5581 acgtcgatgc ccttgccgac gcgctcgtcg aagcggtcaa cgatgacgcc gccaggaagg
     5641 aacgcgccga ccatgccagg cagcagattc gcggcgcgtt ttcctggcga gcgctggcaa
     5701 ggcgcttcga tgccctctac accgacgtgc tcgaccgcgc ttcctagccc gatccaaaac
     5761 tgcgccccgc gagccagagc cgcaatcgcc ttctctgctc gtggcgatct acagcaggat
     5821 cctggcgatg tgctcgggcg tagctccggc ggcgatatgg gccgcggcct cgtgccaatc
     5881 cacgttggat cgcgccatct catccgcgtt ctcgaagccc aggcgcgcaa actgaagcgc
     5941 gcgccactga atcgtcaggt catcgtggct gtcaccggta cctacctcgg cagccgcctg
     6001 aagatggacg cccataggaa gcagtctacg accgtgtggg gtgcacgaac tttggttctc
     6061 atgcttggcc gccgcctacc gcacaggcga gctggagttg gagttacccc gtttccgttt
     6121 gtcccggccc cctctattcg gtctcctcgc aacgtcgtac ccatccgctc gtgagagatc
     6181 gagtaaggcg agcgatgtcc gcgaagcgct acagcgtgga gcagatcgtc gccaagctga
     6241 gggaggctga gaagctcctg gcccagggag cgacgagccc aagtgtctct tcgatggaca
     6301 gtcgcagcag gacgtagccg ccgcccggcg acttcccaag ttgaccctgt gttggacccg
     6361 cagacctatc gagtgaggag cgattcatgc cgattccagg gcatttcctc atgcgcctgg
     6421 gaggattcga acctccgtcc ttcggattag aagtccggct ttggcccttc ggttagcggc
     6481 ggatccgcgc ggttgcttcg gattgggcac tcgtgggtgg aagcgagccg gcggctcggt
     6541 gaatcggctt gacaagctct cgatcgtggt gggaagctgc gcatacgaat acgagtgcag
     6601 cgcgaccccg tttgcgaggg tgtcgcatag gggctgactg tggccgccgc ctcgcgtttc
     6661 gagaggaggt atgcctgcac ctcggactca ctaccaggat tcaaatcggg tggggtcgac
     6721 gttagagcgt cggcggcgtg gcacgg
//
LOCUS       DASVEU010000139        15337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1258208,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000139 DASVEU010000000
VERSION     DASVEU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258208"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VF225_05510"
     CDS             complement(<1..685)
                     /locus_tag="VF225_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144748.1"
                     /translation="MAHAKAALETDDARLLNRELSWLDYNARVLARAADRALPLLERT
                     RNCAYFSSNLDEFFQVRVAGLMGQEEAGLLVRSPDGRTPQQTLAEIRERTVELTSDQA
                     KLWKRELRPALAEAGILVGDVEDCNRKELDELDQRFDRDIFPILTPLAVGPGQPFPYI
                     SPLSLSLGIFVRDEETGEERFARVKVPELLPRFLAIGERGLLLPLERVIRHFLPRLFP
                     GMEIVECCTF"
     gene            1281..2066
                     /locus_tag="VF225_05515"
     CDS             1281..2066
                     /locus_tag="VF225_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7144749.1"
                     /translation="MDPAVFLTQFEESNTAAVPRVGGMARANGVVIVSERYWAFAGTD
                     GSLREGRMPVAPRSLRAIPLARGLVRLSGSLSPLFKREGVARRRERGFLIAAILAPLV
                     LAFAPNWLGLTAGFVTTFALLGWLLRGRTLYLHGAEHRAIAALEARRLRETWKGLARP
                     SRFSLRCGTNFAALLLPVAIVGGRFWPLPATAVTPLVVSVLALALTMELWQLVQSLPR
                     LARIVLLPGLGLQRLTTREPRLDETRVALTALAAVLRRELAHG"
     gene            2077..3087
                     /locus_tag="VF225_05520"
     CDS             2077..3087
                     /locus_tag="VF225_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7144750.1"
                     /translation="MDAPRENSIEAHGLVREFKKGPRAVDGIDLRVDPGEIYGFLGPN
                     GAGKSTTVHMLTTLLPPTAGTATVAGYDVVKEGPQVRKAIGAALQEAALDPFLTGREH
                     LRLQTTMHGITGEERRERSDELLTRVGLTEAADRKVKGYSGGMKRRLDLALALVHRPR
                     ILFLDEPTTGLDVQSRTALWVEVGRLAKEEGVTVFLTTQYLEEADVLADRVGIIDHGR
                     IVAEGTPEKLKADIACSTVEAVPGDPDQRGAVVSVLERFGEQTTGYGPGSIAVQLTSS
                     NGNLAEIVRALDAEGIVLSQLQIHEPTLDDVFLAKTGRHLEGAGDEEEQESEPELEPA
                     RA"
     gene            3084..3863
                     /locus_tag="VF225_05525"
     CDS             3084..3863
                     /locus_tag="VF225_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144751.1"
                     /translation="MTATALVQTAALSKRAVVRTARQPANVVFPLIFPMLLFAVNAGG
                     LKAETQLPGFPTSSFVAFALAVPFVQGALLATINANVEFARDIQTGFLNRLALTPVRG
                     IVLLFGQLGGLVTIAVVQAVFYLAVGLAFGVRLQSGVAGAAMLLLLETCIALAFASLG
                     SWAALRTGSTEAVQGLFPVFFVFLFLSSMNIPRNLIEADWFRWVATVNPVSYLIEAVR
                     ALVITGWDWETIGLGFAVAAGLTIAGFALATHALWNRLERT"
     gene            3860..4630
                     /locus_tag="VF225_05530"
     CDS             3860..4630
                     /locus_tag="VF225_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144752.1"
                     /translation="MTDAISVARGVSWRVLHTFFTNKAFLLPSVMFPLFFFTAFVGGL
                     SRISQVPGFDFPTGYTAFQFVFVLLQSAAFAGVFTGFGIARDFETGFMRRLMLAAPNR
                     SGILLGYVFSALVRWATVAVVVTIVALLAQMKIGGNGVDLFGLYVLALLLNLAGALWA
                     AGVAMRLRSQQAAPVMQMPVFLLLFFAPVYVPLNLLEGWIHGVATVNPITALLEAGRG
                     FISGRPELTLAAFAIAAGLILGFAAWAFRGLRRAEAAG"
     gene            4723..5688
                     /locus_tag="VF225_05535"
     CDS             4723..5688
                     /locus_tag="VF225_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144753.1"
                     /translation="MEPEAWPTPQTPPREPLPRVEDLPVAEQGYEQESVKAAFESFYR
                     HAAQLDAALRTLEAVDSFHRQAASLRTDLRALRAAGWTQQSWTQPPSYGYGIRGPREG
                     VPPALWRIGGEVGFLVAVAVALGVAKLSWWVIVLAMAGAFAIVCLIEWLAAREQSSFV
                     RPAVPAPGHPVVEAQRAGDTDEETDEQNSFGWSAFEEAQEPSDAMTIIGAPLKEEEAV
                     AEAEPDPDPEPESEEPELPPAAEADVEAKETHSAARGRWRRGQEDRDTSSQPEPPRHV
                     RVLPPADEPVHLDPWEQGFDGSEAADASDDSALAKDDPPRRFRRR"
     gene            5733..6323
                     /locus_tag="VF225_05540"
     CDS             5733..6323
                     /locus_tag="VF225_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144754.1"
                     /translation="MRKRPIGQILVEKNEISEAELAAALAEQERTGLRLGQILIETGR
                     ISWLALARAIAEQVLDIQEVEPAATPTPPPVAAPPVAAQPQIPTPVAAPAPAVFAPPP
                     TFAVPAPVPVAPAPMPVAPVPAPVAPAPAPVAPQLIHDPEIKLHSVEALLKERQRAFI
                     ELVTTTETLRMKVAHLEEIIEERDRELTKLRVSRAS"
     gene            complement(6327..7811)
                     /locus_tag="VF225_05545"
     CDS             complement(6327..7811)
                     /locus_tag="VF225_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX7144755.1"
                     /translation="MKLAAFAIVLLTLPLAGCDDSKKTSAVQAATTTQAATPPPARTY
                     HSRPDLKPPLVRIRTAAHDTAPGYVFIAPKMVVAQAGPMIMDNRGEVVWFKPLNTRGV
                     TDFRVQHYRGKPVLTWWRGHVSNVGVGDGWYVIYDTSYHPVAEVRPGNGLVGDVHEFL
                     ITKNDTALFTVYHRLAVDLSSVGGPKKGSIWDGIVQEVDIATGRVLFEWHSYPQVGIT
                     ESYAPPPKASAGAKAAPYDYFHVNSIDMEPSGNLLVSARNTHAVYEIEHKTKKVIWRL
                     GGKKSDFAMGPGTRFAWQHDARRQADGTITIFDNGAAPPVEKFSRVLVLRANATTKKT
                     TLVRSYHHPQRLLAPFEGNAQFLPNGDIVVGWGAQPYVSEFARSGALLFDVYFGQGKP
                     PGKDADTYRAYRFPWHGQPRDRPVVVVAGDTAYSSWNGATDVTRWQVLAGPAAGQLKP
                     LQTVAKNGFETPIRLTKSAAFYAVKALGRDGKVLGTSEAVAPRP"
     gene            complement(7854..9086)
                     /locus_tag="VF225_05550"
     CDS             complement(7854..9086)
                     /locus_tag="VF225_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7144756.1"
                     /translation="MSGSPPSLPIRRNTVILSATMAVNSAVLQLTAAVSSLTFVLVTG
                     VRGLLGLGPAIFLTSSGLTAVPAGRLMDRVGRMPVITAGFALGACGCALTALATRTES
                     TIAVIAGFALVGASSGISLLIRTAAGDMYPPTRRARGISYVLFGSVFGAVLGPTVFGP
                     LFAGKDVKADALTVPWFAAGGLALVALALVQFVRPDPKRIAELIGTTDDMPMEPAAPL
                     RELVGRPGVRPAMLAALASFGVMVSVMNLTGYVVVEHHHHSQHSVFPIIGAHVFGMYA
                     LVLVIGALIDRVGRAPALQIGLVVMAISTIGLLWVASVWATAVPLLGLGLGWNFSFVA
                     ATAQLADHTSPGERGKLLGFNDLLGSLFGASLALLGGYALDALGVGALAIGATAIVVA
                     PVLLISRTAASPPAREPA"
     gene            9131..9703
                     /locus_tag="VF225_05555"
     CDS             9131..9703
                     /locus_tag="VF225_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144757.1"
                     /translation="MMLVPDLRPSVAAGCLAVALCAAGPASSPAAAATCNGFGVTPAQ
                     IGGTNFKSSPVRHSVVVAGTKGTRITLSGYVLTSACRAIRGARVDFWQADANGAYDDT
                     GVRLRSHQFTDARGHYWLQTILPGPYANRTRHLHVTVHARGEHTVTTMLYFPGVPLNA
                     HDPFLDRRLLVRLRIVKHTFQARFDFVLSR"
     gene            9700..10533
                     /locus_tag="VF225_05560"
     CDS             9700..10533
                     /locus_tag="VF225_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144758.1"
                     /translation="MKRTAALAGLIFLLVGCGSSGTSQLTVGAARTYALAGFAPTRFS
                     AGKPTRLSFTIDQPSGKPLTSYKTGPGPHTGVHLIIVRSDLGALIHDHPPVGADGRIS
                     ETITLPTPGRYRVVVDAYPNLSGPLRNFQLFGVITAPGTAPDEGLGPFRRTAVVSGYR
                     FTLTNRPRLKAISPAFLTLDVARPDGAPATFTPWFGALAHAIFFRSGSLDYFHTHVCS
                     AGMTGCTSILGSTRITGRATKPGQLRVGVLVPVPGTWRLFLQCKVDGRVLTAPFTLVV
                     R"
     gene            10530..11159
                     /locus_tag="VF225_05565"
     CDS             10530..11159
                     /locus_tag="VF225_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144759.1"
                     /translation="MSVIRLLSRLVVAALVVLMARSIGYALAPSPAARVFEHRAGGPA
                     LPVLTLVVLAVGCSLAVAICWLAALGVRERELLERRRLAATPPRLRPVRTLALALALS
                     VVTSAVGGLLEAYLHRRAGLGWHGMHCVFGPVHRNLLPIATSLSFVAAALLAAAEHVV
                     GWMRRTFALLRALPPRLVRAELPSWVLVDLGRASRRFGAGTARAPPALP"
     gene            11192..11788
                     /locus_tag="VF225_05570"
     CDS             11192..11788
                     /locus_tag="VF225_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEX7144760.1"
                     /translation="MKQLRARTRWVVLPVAAGGALMLAGSAFAHAEVSPTTVVSKKTE
                     FFTLAVPTEKENAATTQVEFTPPSGFSIDSFAPTPGWKREAQTTGTGENTVVQKVTWT
                     GGKVPTEEDAVFQFLASTDAAKTYAFKVRQTYSDGTVVDWTGPESSDTPAPTIEAKSS
                     LGGGGSNTIGIVALALAVLALVAAVAGLVGKSGRRTLA"
     gene            11785..13515
                     /locus_tag="VF225_05575"
     CDS             11785..13515
                     /locus_tag="VF225_05575"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX7144761.1"
                     /translation="MTRRARIVLLLVVVAGALALPAAAWAHAVLVRTSPLPSSVVNRP
                     PPLVLLTYSEAVEPRFAVVSVTNAAGHQQIAGPPRRSATDPTTLVVPLHRLSQGWYLV
                     YWRVISVDGHPVRGAFTFAVGPNPGPIPQFPVPSISETAATPSLVTARAIVFLSVMVA
                     IGLFILRIATARPVVRRVSETRLRAVSVAFGIAAAIALIATPVYVVMATAQFALRSVF
                     DLGNVVPLVRDSAFGRGYLDLELTFALFVLAAALALWVDRPERPQRSIAELLSNAGAL
                     LAGGATLLVPGLSGHAAQTSPRGLSVALDWLHLVAGSVWIGGLIGLLVLWWSLPVARR
                     VAGLMVCVPRFSNTAFVSVLALVGSGVGASIIHLPTFSSLWQTSYGQALLVKIGLLCA
                     ALLIAAVNLLRTKPRLAASHDRPEFGTGTAALLRRLVSGEVLLVSAAVVAAAVLSSLP
                     PPAKALATAGSAVARVGPGPVAEVVNKNGYRLALHIAPNRAAAPNTFAISVTKGGRPL
                     RHATVIAGFAMLDMEMGTQTHVLPERAPGLYELSTPALVMVGHWGLSFEVTPPGQQPF
                     TVVFVDRTTG"
     gene            13609..14226
                     /locus_tag="VF225_05580"
     CDS             13609..14226
                     /locus_tag="VF225_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144762.1"
                     /translation="MARRLVALIGVAVCLSAPSLARANGDPASDYLLAQNLFLPFTTK
                     IDQNSVKRLNAVLAEAKAQRFPIRVALILSPSDLGTAYSLFGKPEKYAQFLGLELAFV
                     YRDRLLVVMPSGYGYAVKGDPDPKASAVLKKLSAPGRSATKEADAAIVAVQRLAAREG
                     HRLAAPKVGGSAGRDRLTIAAAATAGIALIAAIVLYRRREKTAGA"
     gene            14259..>15337
                     /locus_tag="VF225_05585"
     CDS             14259..>15337
                     /locus_tag="VF225_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7144763.1"
                     /translation="MTICPSCGQENPPIAKFCLACAQPLSEESPGLGEERKVVTVLFA
                     DLVGFTARAERLDPEDVRAILTPYFARIRSELEKFGGTVEKFIGDAVMAIFGAPVAHG
                     DDPERAVRAALAMCAAVDELNRNDPELELRIRVAVNTGEALVSLGASAAQGEGVVAGD
                     VVNTASRLQEAAPVNGILVGEETYRATRAVIRYEDAEPVVAKGKQEPVRVWRAVAASA
                     GPGERAAGRVPMLGRVSELAALQRIWDNVLAEQRPQLVTLFGPAGIGKTRLASEFAEL
                     AAAGGARVIKGRSLPYGEVIPYAAFTAQVKQVAKIFDTDEAGTACEKLDRAVVELLGD
                     EAQETSAHIAMLIGIGKGGDVGNRQT"
BASE COUNT         2283 a         5138 c         5162 g         2754 t
ORIGIN      
        1 ggaacgtgca gcattcgacg atctccatgc ccgggaacag acgcggcagg aagtgtctga
       61 tcacccgctc gagcggcagc aggagcccgc gctcgccgat cgcgaggaac cgcggcagca
      121 gctcgggcac cttcacacgt gcgaagcgct cttcgccggt ctcctcgtcc cggacgaaga
      181 ttccgaggct gagggagaga ggcgagatgt acgggaacgg ctggcccggc ccgaccgcga
      241 gcggggtcag gatgggaaag atgtcgcggt cgaaacgctg gtcgagctcg tcgagctcct
      301 tgcggttgca gtcctcgacg tcgcccacga ggattccggc ttccgcgagt gccgggcgga
      361 gctcccgttt ccagagcttc gcctggtcgg acgtcagctc gaccgtgcgt tctcgaatct
      421 ctgcgagcgt ttgttgcggg gtccgcccgt cgggggagcg gacgagcagg ccggcttcct
      481 cctggcccat caggccggcg acccgcacct ggaagaactc gtcgagattc gacgagaagt
      541 acgcacaatt acgcgtccgc tcgagtagcg ggagcgccct gtccgccgca cgggcgagaa
      601 cccgagcgtt gtagtcgagc cacgacagct cccggttgag cagtcgcgcg tcgtccgtct
      661 cgagagccgc tttggcgtgg gccacgagtt atcttgcctg aatctggggc gcctatgggt
      721 tacagacccg tgacagttcc cctcccggga cgcgatcggc agcccgacga acgaggaaga
      781 cccccggtgc ggccggaggt cttcgtcgtc cgggtggaaa aggcggtgcg ttacttctcg
      841 gggaacttgc cgggtggcag gtgcatggcc actggccgtt caccgcgatg ttgtcgagca
      901 tctgccggcc ttcggttccg tccagcagtg cgaggcgagt ttcccggcgg gcaggcggtt
      961 gatgggctga cggctggtga accttcggtg aggaacctga ggccggcgct acccgggtga
     1021 cgcttccggt cacgtaaccg ggccgggtgt tttctcgttg atcccaggcg gaatcactct
     1081 gggcaggtcg acaatcgaca ccagaaaggg aggcacgaaa tcgcatgagc atcctctgga
     1141 tcatcctcat cgtgatcctc gtcctcgcgc tgctcggctt cttctcgcgc gggcgctggt
     1201 aagcacctag gaccaacaaa ggttcgaaat aggccgcccg gcacttgccg ggcggccctt
     1261 cctgtggcga tcatggctgg gtggatcccg ccgtcttcct cacgcagttc gaagagagca
     1321 acaccgccgc cgtgcctcgt gtgggcggaa tggcgcgtgc caacggcgtc gtgatcgtca
     1381 gcgagcgcta ctgggccttc gcggggacag acggaagctt gagggaaggg cggatgcccg
     1441 tagcgccacg ctcgcttcgc gcgattccac tcgcgcgcgg tctcgtgcgg ctctcggggt
     1501 cgctgtcgcc cctcttcaag cgtgaggggg tcgcgcgccg tcgcgaacgg ggttttctga
     1561 tcgcggcgat tctcgctccg ctcgtacttg cctttgcacc gaattggctc ggtctcaccg
     1621 cgggattcgt cacgacgttc gcgctgctcg ggtggctctt gcggggccgc acgctctacc
     1681 tgcacggcgc cgaacaccgc gcaatcgcag cgctcgaggc acggcgtctt cgggagactt
     1741 ggaagggcct tgcgcggccc tcgcgcttct cgcttcgctg cggcaccaac tttgcggcgc
     1801 tgttgctccc cgtggcgatc gtcggcggcc gcttctggcc gttgcccgca accgcggtca
     1861 ctccgctcgt cgtgtccgtc ctcgcgttgg cgctcacgat ggagctctgg cagctcgttc
     1921 agagcctgcc tcgactggcc cgcatcgtgc tcctgccggg cctgggacta cagcgtctca
     1981 cgacgcgtga gccgaggctc gacgagactc gcgtcgcgtt gaccgcgctt gctgcagttc
     2041 tccgtcgaga actcgcgcac gggtagcctc cggggcgtgg acgccccgcg cgaaaacagc
     2101 atcgaggccc atgggctcgt ccgcgagttc aagaagggcc cgcgcgccgt cgacggcatc
     2161 gatctccgcg tcgaccccgg tgagatctac gggtttctcg gcccgaacgg cgccgggaaa
     2221 tccacgaccg tccacatgct gacgacgttg ctgccgccga ccgcgggcac cgcgacggtc
     2281 gccggttatg acgtcgtcaa ggagggcccg caggtccgga aagcgatcgg ggccgcgctc
     2341 caggaggccg cgctcgatcc gttcctgacg ggacgagagc acctcaggct gcagaccacc
     2401 atgcacggga tcaccggcga ggagcgacgc gaacggagcg acgagctact cacccgggtc
     2461 gggctgaccg aggcggcgga ccgcaaggtg aagggctatt ccggtggaat gaagcgtcgt
     2521 ctcgaccttg cgctggcgct ggtgcaccgg ccgcgcatcc tctttctcga cgagccgaca
     2581 acggggctcg atgtccagag ccgcaccgcc ctctgggtcg aggttggccg gctcgccaag
     2641 gaagaggggg taaccgtgtt cctgacgacg cagtacctcg aagaggcgga cgtgcttgcg
     2701 gaccgcgtcg ggatcatcga ccacggacgc atcgtggcgg aggggactcc cgagaagctg
     2761 aaggcggaca tcgcctgctc cacggtcgaa gccgtcccgg gcgaccccga ccagcggggc
     2821 gcggtggtat cggtgctcga gcgcttcggc gagcagacga ccggttatgg gcctggcagc
     2881 atcgccgtcc agttgacgtc gtcgaacggc aatctcgccg agatcgtccg cgcgctcgac
     2941 gcggaaggca tcgttctctc tcagctgcag atccacgagc cgacgctcga cgacgtcttc
     3001 ctcgcgaaga ctgggcgcca tctcgagggc gccggggacg aggaggaaca ggagtccgaa
     3061 ccagaactcg agcccgcccg cgcgtgaccg ccacagctct cgtccagacc gcggcgctga
     3121 gcaagcgcgc ggttgtccga accgcgcggc agcccgcgaa cgtcgtcttc ccgctgatct
     3181 tcccgatgct tctgttcgcc gtgaacgcgg gtggtctgaa ggccgagacg caacttcccg
     3241 gctttcccac cagctcgttc gtcgcgttcg cgctcgccgt gccgttcgtc cagggagcac
     3301 tgctcgcgac gatcaacgcg aacgtcgagt tcgcgcggga catccagacc ggtttcctca
     3361 acaggctggc gctgaccccg gtgcgaggaa tcgtcctgct cttcggacag ctgggcggat
     3421 tggtgacaat cgctgtcgtc caggcggtct tctacctcgc cgtcgggctc gcattcgggg
     3481 tgcgtttaca gagcggagtc gccggggcag cgatgttgct actgctggaa acctgtatcg
     3541 cgctcgcgtt cgcgtcgctc ggcagctggg ccgcgctgcg gacaggctcg actgaggcgg
     3601 tgcagggcct ctttcccgtc ttcttcgtct ttctgttcct gtcgtcgatg aacatcccgc
     3661 gcaacctgat cgaggcggac tggtttcggt gggtggcgac ggtcaacccg gtctcgtacc
     3721 tgatcgaggc ggtccgcgca ctcgtgatca cggggtggga ctgggagacc attggcctcg
     3781 gcttcgctgt cgccgccgga ctgaccatcg ccggcttcgc ccttgcgacc cacgcactct
     3841 ggaaccgttt ggagcgcaca tgacagacgc gatttcagtt gcgcgcggcg tgtcgtggcg
     3901 cgtcctccat acgttcttca cgaacaaagc gttcctgctg ccttcggtga tgtttccgct
     3961 tttcttcttc accgcgttcg tcggtggctt gtcgcgtatc agccaggtgc cggggttcga
     4021 ctttccgacc ggctatacgg cgtttcagtt cgtgtttgtg ctgcttcagt ccgcggcctt
     4081 cgcgggcgtg ttcaccggat tcggaatcgc acgtgacttc gagaccggct tcatgcgacg
     4141 gctgatgctt gcggccccga accggagtgg catcttgctc ggatacgtgt tctcggcgct
     4201 cgtacgctgg gcgaccgtcg ccgtggtcgt gacgatcgtg gccctgctcg cgcagatgaa
     4261 gatcggcggc aacggcgtcg acctgttcgg tctctatgtg ctcgcgctgc tcctgaatct
     4321 cgccggggct ctctgggccg ccggcgtcgc catgcgcctg cgatcgcagc aagctgcacc
     4381 cgtgatgcag atgccggttt ttctcctgct cttcttcgct ccggtgtacg tcccgctcaa
     4441 tctgctcgag ggctggatcc acggcgtcgc taccgtcaac ccgatcacgg ccttgctgga
     4501 agccggtcgg ggcttcatct ccggcaggcc ggagctgaca ctggcggctt ttgccatcgc
     4561 ggccgggctg atccttggat tcgccgcctg ggcctttcgt ggcctcagac gtgcggaagc
     4621 cgccggctga gcgccgagcg aagagttccg gggctgttct cccacgcggc ctccatgtga
     4681 agcgcctagt cgtcgcctgc ttgaacgcat aatgtcctgc taatggagcc cgaagcctgg
     4741 ccgacgccgc agacgcctcc gcgcgagccg ttgccgcgcg tcgaggatct acccgtcgcg
     4801 gagcagggct acgagcagga aagtgtcaag gctgcgttcg agtcgttcta ccgtcatgcg
     4861 gctcaactcg atgcggcctt gcgaacactc gaggccgtgg attcatttca ccggcaggcc
     4921 gcgtccttgc gcaccgacct gcgtgctctc cgtgcagcgg ggtggacgca gcagtcgtgg
     4981 acacagccgc cgtcctacgg ctacgggatc cgtgggccac gcgagggagt tccgccggcc
     5041 ctctggcgca tcggcggcga ggttggattc cttgtcgccg tggcggtggc gctgggcgtg
     5101 gcgaagcttt cctggtgggt gatcgtcctc gccatggccg gtgcgttcgc gatcgtctgc
     5161 ctgatcgagt ggctcgccgc gcgcgagcag tcgtcgttcg tgcgacccgc agttccggct
     5221 cccgggcacc cggtcgtcga ggcccagcgg gcgggagaca cggacgagga aaccgacgaa
     5281 cagaactcat tcggctggtc cgccttcgag gaggcgcaag agccctcgga cgcgatgacg
     5341 atcatcggcg ctcccctgaa ggaggaggaa gcggtcgccg aggcggagcc cgatccggat
     5401 ccggagcccg agtcggagga gccggagttg ccacccgcgg cagaggcgga tgtagaggcc
     5461 aaggagactc actccgcagc tcgtggccgg tggcgccggg gtcaggagga ccgcgacaca
     5521 agctcccagc cggagccgcc tcggcatgtc cgcgtgctcc cgccggctga cgagccggtg
     5581 cacctggacc cgtgggagca gggcttcgac ggctcggagg ccgccgacgc gagcgacgat
     5641 tcagccctgg ccaaggacga ccctccgcgc cgcttcagac gtcgctagac gaggcccttt
     5701 cgtccggcgg tgttcctgcc gataggagtc caatgcggaa gcgtccgatc ggccagattc
     5761 tggtcgagaa gaacgagatc tctgaggccg agctcgcggc tgcgctcgcc gaacaggagc
     5821 ggaccgggct gcgcctgggc cagatcctga tcgagaccgg gcggatctcc tggctcgcgc
     5881 tcgctcgagc aatcgccgag caggtgctcg acatccagga agtcgagcct gcggctacac
     5941 ctacaccccc accggtcgca gcgccgccgg tcgcagctca gccgcagatc ccgacccccg
     6001 tggccgcccc tgcacctgcg gtcttcgcgc ccccgccgac cttcgcggtg cccgccccgg
     6061 tccccgtcgc acccgccccg atgcccgtcg cccccgtgcc tgcgcctgtc gcccccgctc
     6121 ctgcacccgt ggccccgcag ttgatccatg acccggagat caagctgcac agcgtcgagg
     6181 ccctcctgaa ggaacgccag cgcgcattca tcgagcttgt caccaccacg gagacgcttc
     6241 gcatgaaggt cgcccacctc gaagagatca tcgaggagcg cgaccgcgag ttgacgaagt
     6301 tgcgcgtctc ccgcgcgagt tagtacctag ggccgcggcg cgaccgcttc ggacgtcccg
     6361 agcacctttc cgtctcggcc gagagccttc accgcgtaga aggccgccga tttcgtgagc
     6421 cggatgggag tctcgaagcc gttcttcgcg acggtctgca acggcttcag ctgaccggcc
     6481 gccgggccgg cgagcacctg ccaccgcgtc acgtccgtgg ccccgttcca actcgagtag
     6541 gcggtgtcac cggcgaccac gaccacaggt cggtctctcg gctgcccgtg ccaggggaac
     6601 cggtacgcgc ggtaggtgtc ggcgtccttg cccggcggct tgccctggcc gaagtagacg
     6661 tcgaagagaa gagccccgct gcgcgcgaac tcgctcacgt acggctgcgc tccccacccg
     6721 acgacgatgt cgccgttcgg caggaactgc gcgttgccct cgaagggagc cagtagccgc
     6781 tgcggatggt ggtagctgcg cacgagtgtg gtcttcttcg tggtcgcgtt cgctcgcagg
     6841 accagcactc gcgagaactt ctccaccggt ggagctgcac cgttgtcgaa gatcgtgatc
     6901 gttccgtcgg cctggcgtcg tgcgtcgtgc tgccacgcga atctcgtgcc cgggcccatc
     6961 gcgaagtcgc tcttcttgcc acccagccgc cagatgacct tcttcgtctt gtgctcgatc
     7021 tcgtagacgg catgcgtgtt tcgtgcggag acgagcaggt tgccgctcgg ctccatgtcg
     7081 atggaattga cgtggaagta gtcgtaaggc gccgccttcg cgcccgcgct cgccttcggc
     7141 ggtggcgcat aggactccgt gatcccaacc tgggggtagc tgtgccattc gaagagaact
     7201 cgccccgtcg cgatgtcgac ttcttgcacg attccgtccc agatgctgcc cttcttcggc
     7261 ccgccgaccg aggagaggtc gaccgctagg cggtggtaca cggtgaagag ggcagtgtcg
     7321 ttcttcgtga tcaggaactc gtgcacgtcg ccgacgagcc cgttgccggg cctgacctct
     7381 gcgacagggt gatacgacgt gtcgtagatc acgtaccagc cgtcaccgac gccgacattc
     7441 gagacgtgtc cccgccacca ggtcaacaca ggcttgccgc ggtagtgctg gacgcggaag
     7501 tccgtgaccc cgcgagtgtt cagcggcttg aaccagacga cctcgccacg gttgtccatg
     7561 atcatcgggc cggcctgcgc cacgaccatc ttcggcgcga tgaagacata gccgggcgcg
     7621 gtgtcgtggg ccgcggtccg gatccggacc agcggtggct tcaggtccgg acgcgagtgg
     7681 taagtgcgcg cgggcggcgg cgttgccgct tgcgtcgtcg tggccgcctg cacggcgctc
     7741 gtcttcttcg agtcatcgca gccggcgagc ggcaacgtca ggaggacgat tgcgaaagcg
     7801 gccagcttca cgcgcaagag catagacagg tcgcggcggt gttagccctc gcttcaggcc
     7861 ggctcgcgcg caggcggact ggctgcggtg cgcgagatca ggagaacggg cgcgaccact
     7921 atggccgttg cgccgatcgc cagcgctccc acgccgagcg cgtcgagcgc atacccgccg
     7981 agtagggcga ggcttgcacc gaagagcgag ccgagcaggt cgttgaaacc gagcagcttg
     8041 ccgcgctcgc ccgggctcgt gtgatctgca agctgtgcgg tcgccgccac gaaggagaag
     8101 ttccacccaa gcccgagccc gagcagcggg acggcggtcg cccacacgct cgcgacccag
     8161 agcagtccga tcgtcgaaat cgccatcacg acgagaccga tctggagtgc gggcgcacgc
     8221 ccgactcggt cgatcagtgc cccgatcacg aggacgaggg cgtacatccc gaagacgtgt
     8281 gcgccgatga ttggaaacac cgagtgctgg ctgtgatggt gatgctcgac gacgacgtag
     8341 ccggtgaggt tcatcaccga gaccatcacg ccaaagcttg cgagtgcggc gagcattgcg
     8401 ggtcgaacgc cgggccggcc cacgagctcg cgaagtgggg cggcaggctc catcggcatg
     8461 tcgtccgtcg ttccgatgag ctcggcgatt cgcttcgggt cgggccggac gaattgcacg
     8521 agcgcgagtg cgaccagcgc gagcccaccg gctgcgaacc agggaacggt caaggcgtcg
     8581 gccttcacgt ccttgccggc gaagagcgga ccgaaaacgg tcggtccgag aacggcgccg
     8641 aagaccgacc cgaacaagac gtacgagatc ccgcgcgcgc gccgtgtggg cggatacatg
     8701 tcacctgcgg ctgtgcggat gaggagcgag ataccactcg acgcgccgac gagcgcgaag
     8761 cctgcgatga ccgcgatcgt cgactcggtg cgtgtcgcga gcgctgtgag tgcgcacccg
     8821 cacgcaccga gagcgaagcc ggccgttatc accggcatgc ggccgacccg gtccatcagg
     8881 cggccggccg gcacggcggt caatcccgag gaggtgagga agatggccgg gccgaggccg
     8941 agcagccctc tcacgccggt gacgagcacg aacgtcagcg acgagacggc ggcggtcaac
     9001 tggaggacgg cggagttgac cgccatcgtg gccgagagga tcaccgtatt gcgccgaatc
     9061 ggcaggctcg gcggggatcc gctcacgacc ggttcattca atcaggcacc gtcctggtgc
     9121 gattgcttca atgatgctcg tgccggacct gcgcccgagc gtcgccgccg gatgtttggc
     9181 ggtagccctg tgcgccgcag gccccgcttc gtcaccggcc gccgccgcta cgtgcaatgg
     9241 cttcggcgtc actcctgcgc agatcggcgg aacgaacttc aagtcgtcgc ccgtgcgcca
     9301 ctcggtcgtt gtcgccggta ccaagggaac gcgcattacg ctcagcgggt acgtgctgac
     9361 gagtgcgtgt cgtgcaatcc gcggcgcgcg cgtcgatttc tggcaagcgg acgccaacgg
     9421 agcgtacgac gacaccggcg tccgtttgcg gagtcaccag ttcaccgatg cccgagggca
     9481 ctactggctt cagacgatcc tgcccgggcc gtatgcgaat cgcacgcgcc acctgcacgt
     9541 gaccgtgcac gcgcgtggtg agcacacggt cacgacgatg ctctacttcc ccggcgtgcc
     9601 attgaacgcg cacgacccgt tcctcgatcg ccgactcctc gtgaggcttc ggatcgtcaa
     9661 acacaccttc caggcgcggt tcgacttcgt cttgtcccga tgaaacgcac ggcggcgctc
     9721 gccggtctca tctttctgct cgtaggctgc ggctcgtcgg gtacatccca actgaccgtc
     9781 ggcgccgccc ggacgtacgc gctcgcaggc ttcgctccca cgaggttcag cgcggggaag
     9841 ccgacccggc tctcgttcac gatcgaccag ccgtcaggga aaccgctcac gagctacaag
     9901 acgggaccgg gaccgcatac gggagttcac ctcatcatcg tgcgcagcga cttgggagct
     9961 ctgatccacg accacccacc ggttggcgct gacggacgga tcagcgagac catcacgctg
    10021 ccgacgcccg ggaggtatcg cgtggttgtc gacgcgtatc cgaacctttc aggcccgctc
    10081 cgaaactttc agctgttcgg ggtgatcact gcgccgggaa ccgccccgga cgaaggcctc
    10141 ggccccttcc gccgcacggc cgttgtctcc ggctaccgct tcaccttgac gaatcggcca
    10201 cggctcaaag cgatcagccc ggcgttcctg acgctcgacg tcgcgcggcc ggacggcgcc
    10261 ccggcaactt tcacgccgtg gttcggagcg ctcgcacacg cgatcttctt ccgctcgggg
    10321 tcgctcgact acttccatac gcatgtctgt tccgcaggaa tgaccggctg cacgagcatt
    10381 ctgggctcga cgcgcatcac gggtagagca accaagcccg gtcagctccg cgtcggcgtg
    10441 ctggttcccg ttccgggtac gtggcggctc ttcctgcagt gcaaggtgga cgggcgtgtg
    10501 ctcaccgctc cgtttactct ggtggtgcga tgagcgtcat tcgcttgctt tcccggctcg
    10561 tcgttgcggc actcgtcgtc ctcatggccc gcagcattgg ctacgcgctc gcgccgagcc
    10621 ccgccgcacg cgtgttcgag caccgggcgg gcggtccagc gctgccggtc ctcacgctcg
    10681 tagtgctcgc agtcggctgt tcgctcgcgg tcgcgatctg ttggctggct gcgttaggtg
    10741 tccgcgagcg tgagctcctg gagcggcgca ggctcgccgc gacgccgcct cggcttcgac
    10801 ccgtgcgcac gctcgcgctt gcgcttgccc tctcggtcgt gacctcggcc gtcggcgggt
    10861 tgcttgaggc ctacctgcac cggcgtgccg ggctgggctg gcacggcatg cactgcgtct
    10921 tcggtccggt tcaccgcaat ctcttgccga tcgcgacctc gctttcgttc gtcgcagccg
    10981 cgctgctcgc agctgcagaa cacgttgtcg ggtggatgcg gcgcaccttc gcgctgctgc
    11041 gcgctctgcc gccgcggctc gttcgcgcag agctcccttc gtgggtcctc gtggacctgg
    11101 ggagagcctc ccgccggttc ggcgccggga ctgcgcgcgc gccgcccgcg ctcccctgac
    11161 gttcacttcc gaaccgaaag gagaagcatc gatgaagcag ttgcgcgcga ggacgcgctg
    11221 ggtcgttctg cccgtggcgg cgggtggtgc gctgatgctc gccggctcgg cctttgctca
    11281 cgcagaagtc agccctacga ctgtggtttc caagaaaacg gagttcttca cgctcgccgt
    11341 gccgacggag aaggagaatg cggccacgac gcaggtcgag ttcacgcctc cgagcggttt
    11401 ctcgatcgac tcgttcgccc cgactccggg gtggaagcga gaggcacaga cgaccggcac
    11461 cggcgagaac actgtcgtcc aaaaggtgac ctggaccggt ggcaaagttc cgaccgagga
    11521 agacgccgtt ttccagttcc tcgcctcgac ggacgcagcg aagacgtatg cgttcaaggt
    11581 gcggcagacg tactcggacg gaacggtcgt cgactggacc ggccctgagt cctcggacac
    11641 gccggcgcct acgatcgagg cgaagtcctc cctcggcggt ggcggtagca acacgatcgg
    11701 gatcgttgcg cttgcgctag ccgtgctcgc gctggtcgca gcggtcgccg gtctcgtcgg
    11761 gaagtctggg agacgcacgc tggcgtgacc aggcgcgcgc gcatcgtcct gttgctcgtg
    11821 gtggtcgcgg gggcgcttgc gcttcccgcg gccgcctggg cgcacgcggt gctcgtgcgc
    11881 acttcgccgc tcccgagctc ggttgtgaat cggccgccgc ccctcgtcct cttgacgtac
    11941 tcggaggcgg tcgagccccg ctttgcggtc gtctccgtca cgaacgcggc ggggcaccag
    12001 cagatcgccg gaccgccgcg gcggtccgca acggatccca caacgcttgt cgtgccgctc
    12061 caccgcctgt cgcaggggtg gtacctcgtc tactggcgtg tgatctccgt cgacggccat
    12121 ccggtccgcg gcgcgttcac attcgcggtc ggccccaatc cggggcccat accgcagttt
    12181 cccgtgcctt cgatctccga gactgccgcg accccgagtc ttgtcaccgc acgcgcgatc
    12241 gtctttctgt ccgtcatggt ggcgatcggc ctcttcattc tgcgcatcgc gaccgcgcgt
    12301 ccggtcgtgc ggcgcgtctc ggagacgcga ctccgcgccg tctcggttgc gtttgggatt
    12361 gcggcagcga ttgcgttgat cgcgactccc gtctacgtcg tgatggcaac ggcccagttt
    12421 gcgctgcgct cggtcttcga tctggggaat gtcgtgccac tcgtgcgcga ctcggcgttc
    12481 ggtcgtggtt atctcgacct cgagctcacc tttgcgctct tcgtgctcgc ggctgcgctc
    12541 gcgctctggg tcgaccggcc cgaacgcccg cagcgctcca ttgcggagtt gctctcgaat
    12601 gccggtgcgt tgctcgccgg cggggcgacc ctgctcgtgc cggggctctc cggccacgct
    12661 gcgcagacct ctccccgggg tttgtcggtg gcactcgact ggctccacct ggtggcaggc
    12721 tcggtctgga tcggcggcct gattgggctg ctcgtgctct ggtggagtct tccggtcgct
    12781 cggcgcgtcg cgggcctcat ggtctgcgtg ccgcggttct cgaataccgc cttcgtctcg
    12841 gtgcttgcgc tggtcggctc cggtgtcggc gcctcgatca tccacttgcc gacgttctcg
    12901 tccctttggc agacctccta cgggcaagcc ctgctcgtca agatcggtct actgtgcgcc
    12961 gccttgctga tcgcggccgt caacttgctc cgcacgaagc cccggctggc tgcgagccac
    13021 gatcgtccgg agttcgggac cggaacggcg gcgctgttac gcaggctcgt gtcgggagaa
    13081 gtcctcctgg tttcggcggc ggttgtcgcg gctgccgttc tctccagcct gccgcctccc
    13141 gccaaggcgc tggccaccgc tggtagtgcc gtcgcccgag tcggacccgg tccggtagcg
    13201 gaagtcgtga acaagaacgg ttaccggctc gcactccaca ttgccccgaa tcgcgccgca
    13261 gcgcccaaca cgttcgcgat cagcgtcacg aagggtggcc ggccgctgcg acatgcgacc
    13321 gtgatcgccg gctttgcgat gctcgacatg gagatgggca ctcagaccca tgttttgcca
    13381 gaacgcgcgc ctgggcttta cgagctctcc accccggcgc tcgtgatggt gggacattgg
    13441 gggctgtcgt tcgaggtcac gccgcccggc cagcaaccgt ttaccgtcgt cttcgtcgac
    13501 cgcacaaccg gatgaaaagc gttcccctgc tgctgctggc ctccctcgtg gcgctcgccg
    13561 cgggagtagg ggcgtgcgtc gtcgtcatcc ttctggcggt cgacgccctt ggctaggcgg
    13621 ctcgtcgctc tgatcggtgt ggctgtgtgt ctcagcgcac cgtcgctcgc ccgggcgaac
    13681 ggcgatccgg cgagcgacta cctgctcgcg cagaacctct tcctgccgtt cacgacgaag
    13741 atcgaccaga acagcgtcaa gcggttgaac gctgttctgg ccgaagcgaa ggcgcaacgt
    13801 ttcccgatcc gggtggcgct gatcctgagc ccgtccgacc tcggcactgc gtattcgctc
    13861 ttcggcaagc cggagaagta cgcccagttc ctcgggctcg agcttgcctt tgtctatcgc
    13921 gaccgcctgc tggtcgtgat gccgagcggc tacggctacg ccgtcaaagg cgatcccgat
    13981 ccgaaggcga gtgcggtgct gaagaagctc tcggccccgg ggcggagcgc aacgaaggag
    14041 gccgacgcgg caatcgtcgc tgtgcagagg ctcgccgcca gggaaggcca ccgtctggcg
    14101 gcgccgaagg tgggtggctc ggcaggccgg gatcgactca cgattgcggc cgcagcgacg
    14161 gccggaatcg cgctgatcgc cgcgatcgtc ctttatcgcc gtcgcgaaaa gaccgccggc
    14221 gcctagaccg cctccggatt cgcccgtacg ctttgaccat gacaatctgt ccgagctgcg
    14281 gtcaagagaa cccgccgatc gcgaagttct gccttgcttg tgcccagcct ctctccgagg
    14341 agtcgccagg cctcggtgag gagcgcaagg ttgtcaccgt gttgttcgcg gatctggtcg
    14401 gcttcactgc tcgggcagag cggctcgatc ccgaggacgt tcgggcgatc ctgacgccgt
    14461 acttcgcgcg tatccgctct gagctcgaga agttcggagg caccgtcgag aagttcatcg
    14521 gcgatgccgt gatggcgatc ttcggagcgc ccgtggcgca cggggacgat ccggagcgcg
    14581 cggtcagagc tgccctcgcg atgtgcgccg ccgtcgatga gctgaatcgc aacgatcccg
    14641 agctcgagtt gaggatccgc gtcgccgtca acacgggcga ggctctcgtg tcgctcggtg
    14701 cgagcgctgc gcagggggag ggcgtcgttg ccggcgacgt cgtcaacacg gcctcgcgtc
    14761 tgcaggaagc tgctccggtg aacgggatcc tcgtaggtga ggagacttat cgagcgaccc
    14821 gtgcggtcat tcgctacgag gacgccgagc cggttgtcgc caaaggcaag caggagcctg
    14881 tgcgtgtttg gcgcgccgtt gcagcttcgg ccggcccggg agagcgtgcc gccggtcgcg
    14941 tcccgatgtt gggacgagtt tcggagctcg ccgcgctgca gcggatctgg gacaacgtcc
    15001 tcgctgagca gcggccgcaa ctcgtcacgt tgttcgggcc cgcgggcatc ggcaagacgc
    15061 ggctggcaag cgagttcgcg gagctcgccg ccgccggagg tgcgcgcgtg atcaaaggga
    15121 gatctcttcc ctacggggag gtgatcccgt acgcggcctt caccgcccag gtgaagcagg
    15181 tggcgaagat cttcgacacg gacgaggcgg ggactgcctg cgagaagctc gaccgggctg
    15241 tggtagagct gctgggcgac gaggcgcaag agacgagcgc tcacatcgcg atgctgatcg
    15301 gcatcgggaa gggaggcgac gtcggcaacc gtcagac
//
LOCUS       DASVEU010000140         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_677399,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000140 DASVEU010000000
VERSION     DASVEU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677399"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1584)
                     /locus_tag="VF225_05590"
     CDS             complement(124..1584)
                     /locus_tag="VF225_05590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7144764.1"
                     /translation="MAPAYGATRHLRPARLGVPANMELRQDTLLAEPTLPAVQLALPP
                     PLAPPWKLGRFRFYLRVRTRFAFTVIAGLVWAGLSTWIALGWIHELAQVVTLPVAVAV
                     IAGIAILPGYLNVQLVSSLLFDRPRPIDFAQDYPDVTLLVAAYNEEAGIRETLEYALR
                     QDYPGRLEVVVADDGSTDATTEIAAAIAADDDRVRVVTTMHAGKSEALNAALLTVRTS
                     VIATIDADTLLMPYALKRAVGRMLSSPPDTVAVAGSVLVRNSRANLLARVQEWDYFLA
                     IASVKRQQALFQGTLVAQGAFSVYRTEAVRRVGGWPDKIGEDIVLTWALIRDGGRTIF
                     EPTAIAFTDAPVRLRHFVRQRRRWARGMIEGLREHGLSLLRGRRLHVHSVAANCLFPY
                     LDTVYTIALPVGLVLALGGNFLIVGPMTAAVLPISLTIALTMYLRQRAVFRSLDLRVR
                     RNLAGFLLYVLAYQLLMSPISVSGYATELMRSRRVW"
     gene            1667..2032
                     /locus_tag="VF225_05595"
     CDS             1667..2032
                     /locus_tag="VF225_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144765.1"
                     /translation="MAVFRYLVGDVDAAIAFYTGNLGFTLEQQMGPAFAIVSRDDLSL
                     WLSGPQSSAARPMPDGRQPQPGGWNRFVVEVEDLESKVAELKEAGLSFRNEIVSGPGG
                     KQIVLEDGDGNPVELFEAR"
     gene            complement(2188..2607)
                     /locus_tag="VF225_05600"
     CDS             complement(2188..2607)
                     /locus_tag="VF225_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144766.1"
                     /translation="MAGKSDFTEQEWESLHKGVVGAGLLVSLSDRSFFDTFKEAGALS
                     KHVVAAKQSSSSELVRELADVRGAGFGLTSSPDKVEHETLEALRSAKTTLESKAPDEL
                     EPYRQFVVDVAQSVADAAGGGEPAESGAIEKVRSAIG"
     gene            complement(2666..3754)
                     /gene="nagA"
                     /locus_tag="VF225_05605"
     CDS             complement(2666..3754)
                     /gene="nagA"
                     /locus_tag="VF225_05605"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7144767.1"
                     /translation="MRLGVGAALVEGKLVPGDVDIADGQVASVGLSSANGTGIAAPGF
                     VDLQVNGFAGVDFFAADTEGYRKAGQALLECGVTAYQPTFITSPEDHLTAALREVPQN
                     GSDPHIIGAHLEGPFIAPERLGTHPAESRRDPDCKLLGRLLDAGPVSHVTLAPELAGA
                     YELMDLLHERRVTVSCGHSNATAAEAREAFARGAKTVTHIFNAMRPFAAREPGLAGAA
                     LFSPDVVVQVILDGVHLADDTARLIWQVASGRVALVTDAIAAAGAGDGSYTLAGVDFD
                     VENGVARRADRVLAGSTVPMIEAVRNLVELGAPVDAALAAATEVPARIAGHAGLGTLQ
                     PGARADVVVLDDSLEIVRVLVGGVDAVG"
     gene            complement(3757..>4483)
                     /locus_tag="VF225_05610"
     CDS             complement(3757..>4483)
                     /locus_tag="VF225_05610"
                     /inference="COORDINATES: protein motif:HMM:NF036518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7144768.1"
                     /translation="DAKLRVQMRAEIARIQQALKVTTVYVTHDQVEAMTMGHRVAVMR
                     DGELQQVDTPQRLYDAPTNLFVASFVGSPPMNLFEAVVERGNGRLVCKVGDTELELPA
                     DVAAERPSLANYTGRRVAVGIRPEDVREASGWDGARLHGRILLVESLGSEQLVHIEIA
                     ATPLERSDLVDTAAQPPGPSLGVDHLERAVTLLGRFDRHLLLAPDEAVEVAIDPRLLH
                     FFDLETGVAIPTVDVPVAAEVAR"
BASE COUNT          765 a         1509 c         1512 g          697 t
ORIGIN      
        1 tagatcgccg ctgcaaggtc ggcggcgctc ttcgatttgt tcttcgtgcc tccgtctcgc
       61 tcggacagga cgttgaaggc ggcggtaagg gaggccagcc ctgacgtagg agctacgggc
      121 gagttaccag actcggcgcg agcgcatgag ctcggtcgcg taccccgaga cggagatcgg
      181 cgacatcagc aactggtacg cgagcacata caggagaaag ccggcgagat ttcgccgcac
      241 gcgcaggtcg agcgaccgga acactgcgcg ctggcggagg tacatcgtca aggcaatcgt
      301 caagctgatc gggagcacgg ccgcggtcat cggaccgacg atgaggaagt tgccgccgag
      361 cgcgaggacg aggccgaccg gcagcgcaat cgtgtagacg gtgtccaggt aagggaagag
      421 gcagttcgcg gccacggagt ggacgtgcag ccggcgccct cggagcaggc tcagcccgtg
      481 ctcacggaga ccctcgatca tcccgcgtgc ccagcggcgc cgctggcgga cgaaatgccg
      541 gagccggacc ggtgcgtccg tgaacgcgat cgcggtcggc tcgaagatcg tgcggccgcc
      601 gtctctgatc agcgcccagg tgaggacgat gtcttcaccg atcttgtcgg gccagccgcc
      661 cactcgccgc acggcctctg tgcggtagac gctgaatgcg ccctgggcaa cgagcgtgcc
      721 ctggaagagg gcctgctgtc gcttgaccga cgcgatcgcg agaaagtaat cccactcctg
      781 cacacgcgcg agcagattcg cccgggagtt gcggacgagc accgaacccg cgacggcgac
      841 cgtgtcggga ggcgaggaaa gcattcgccc gacggcacgc ttcagggcgt acggcatcag
      901 cagggtgtcc gcgtcgatcg tcgctatgac gctcgtacga accgtgagga gcgccgcatt
      961 caacgcttcc gacttgccgg cgtgcatggt tgtcacgacc cgaactcggt cgtcgtcggc
     1021 ggcgatcgca gcggctatct cggtcgtagc gtcggtcgac ccgtcgtcgg cgaccactac
     1081 ctcgagccga ccagggtagt cctgccggag cgcatactcc agcgtctcgc gaatgccggc
     1141 ttcttcgttg tacgcggcga cgagcagcgt gacgtcgggg tagtcctgcg cgaagtcgat
     1201 tgggcgaggg cggtcgaaga ggagcgacga gacgagctgc acgttgaggt aacccgggag
     1261 gatcgcgatt cctgcgatca ccgccacggc cactgggagc gtgaccacct gggcgagttc
     1321 gtggatccag ccgagcgcga tccaggtcga aagaccggcc cagacgagcc cggcgataac
     1381 cgtgaaggcg aagcgggtgc ggacccggag gtagaagcga aagcggccga gtttccaggg
     1441 tggcgcaagc ggcggcggca gcgcaagctg gaccgctgga agggtcggtt ccgcaaggag
     1501 tgtgtcttgc cgcagttcca tgttcgctgg cacgccgagg cgcgcaggcc gcagatgcct
     1561 ggtcgcaccg taggcggggg ccaaggcggc gcctcccccg cccgagggtt tcgcgcggtc
     1621 ctcgatgcgc caccaggcgc acggcaaatg tagattcggt gcactgatgg ccgtctttcg
     1681 ctacctggtc ggcgatgtcg acgccgcgat cgccttctac accggaaacc tcgggttcac
     1741 gctcgaacag cagatgggcc cggccttcgc gatcgtctct cgggacgacc tctcgctctg
     1801 gctcagcggt ccgcagagct cggctgcacg gccgatgccc gatggacgcc aaccacagcc
     1861 cggcggctgg aatcgcttcg tcgtcgaggt cgaggacctc gagagcaagg tcgctgagct
     1921 gaaggaggcc ggcctgagct tccgcaacga gatcgtgagc ggcccaggcg ggaagcagat
     1981 cgtcctcgag gacggtgacg gcaaccccgt cgagctcttc gaagctcgct gatcagcggc
     2041 ctcaggccgg gccacagccg aagaggccga gcgcatgcgg caccgcaggg gtcgaacgaa
     2101 caggaatacc tccccctgac gcttccccgc cgcagctgct cggccctacc tcttgatata
     2161 ggcgagattc gccgacagcg ccactcgcta cccgatcgcg gacctgacct tttcgatcgc
     2221 gccgctctcc gcgggctcgc cgcccccggc ggcgtctgcc acggactgag ccacgtcgac
     2281 gacgaactgc cggtagggct ccagctcgtc cggtgccttc gattcgaggg tggtcttcgc
     2341 cgaccgcaac gcctcgaggg tctcgtgctc gaccttgtcc ggcgatgagg tgaggccgaa
     2401 acccgcgcct cgtacgtccg cgagttcccg tacgagctca ctcgagctgc tttgcttcgc
     2461 cgcgacgacg tgcttgctca acgcgccggc ctccttgaac gtgtcgaaga agctcctgtc
     2521 gctcaaagac acgagcagcc cggcgccgac gacgcccttg tgcagtgact cccattcctg
     2581 ctcggtgaag tccgatttcc cggccatgac tcctccttgt cgtgggttgg gactctggtt
     2641 tcccgacctt agacgctgtg cgccgtcagc cgacggcgtc tacgccaccg acgagcacgc
     2701 gcacgatctc gaggctgtcg tccagcacga cgacatctgc cctcgcgccc ggctgcagcg
     2761 ttccgagccc agcgtggccg gcaatgcgcg ccggaacctc ggtcgctgca gcgagagcgg
     2821 cgtcgaccgg cgctccgagc tcgaccagat ttcgcaccgc ctcgatcatc ggcacggtgc
     2881 tgccggccag aacgcggtcc gcccggcgcg cgacaccgtt ctccacgtcg aagtcgacgc
     2941 cggccagcgt gtaactgccg tctcccgcgc ccgccgccgc aattgcatcg gtcacgagcg
     3001 caacacgtcc tgaggcgacc tgccagatca gccgggcggt gtcgtccgcc agatgcactc
     3061 cgtcgaggat cacctgcacg acgacatcgg gggaaaacaa cgcggcgccg gcgaggccgg
     3121 gctcgcgcgc ggcgaacgga cgcattgcgt tgaagatgtg ggtcaccgtc ttcgctcccc
     3181 gtgcgaaggc ctcgcgagct tctgcggcgg tggcattcga atgaccgcac gagacggtca
     3241 cgcgtcgctc atgcaacaaa tccatcagct cgtacgcacc cgcaagctcg ggcgcgagcg
     3301 tcacgtggct cacagggccg gcgtcgagga gacgtccgag cagcttgcaa tccgggtcgc
     3361 gcctcgactc cgccggatgc gtcccgagcc gctccggggc aatgaacgga ccttcgagat
     3421 gcgcgccgat gatgtgcggg tcggagccat tctgcggaac ctcgcgcagt gccgccgtca
     3481 ggtgatcctc cggagaggtg atgaacgtcg gctgatacgc ggtcacgccg cactcgagca
     3541 acgcttgtcc cgccttccga taaccctccg tgtcggctgc gaagaaatcg acgcccgcga
     3601 acccgttgac ctgcaagtcg acgaaacctg gagcggcaat cccggttccg ttcgccgagc
     3661 tcagcccgac gctcgcgacc tgaccgtcgg caatatcgac gtcgccggga acgagcttgc
     3721 cctcgacgag cgctgccccg acacccaggc gcatgactac ctcgcgacct cggcggcgac
     3781 aggcacgtcc actgtcggga tcgcgacccc ggtctccagg tcgaagaagt ggaggagtcg
     3841 cgggtcgatc gccacctcga ccgcttcgtc cggcgcgagg aggaggtgcc ggtcgaagcg
     3901 gccaaggagc gtcaccgcac gctcgaggtg gtcgacaccc agtgaggggc ctgggggctg
     3961 ggcggcggtg tcgacgagat cggagcgctc gagcggcgtc gctgcgatct cgatgtggac
     4021 gagctgttcc gagccgaggg actcgacgag caggatgcga ccgtggaggc gggcaccgtc
     4081 ccaaccggac gcctcgcgca catcctctgg acggatgccg accgcaacac gtcggcccgt
     4141 gtaattcgca agcgacggcc gctcggcggc gacgtcggcc gggagctcca gctcggtgtc
     4201 gccgactttg cagaccagcc tgccgttgcc gcgctcgacg accgcctcga agagattcat
     4261 cggcggcgat ccgacgaagc tcgcgacgaa cagattcgtc ggggcgtcgt acaggcgctg
     4321 cggcgtgtcg acctgctgca gctcgccgtc gcgcatcacg gcgacgcgat ggcccatcgt
     4381 catcgcctcg acctgatcgt gcgtgacata gacggtcgtc accttgagcg cctgctggat
     4441 gcgggctatc tcggcacgca tctgaacgcg cagcttcgcg tcg
//
LOCUS       DASVEU010000141         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1065516,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000141 DASVEU010000000
VERSION     DASVEU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065516"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..615)
                     /locus_tag="VF225_05615"
     CDS             complement(115..615)
                     /locus_tag="VF225_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144769.1"
                     /translation="MLRKIFVSLLAALATLVVVPSVLAALNSPSVFGASFTHGTVVTG
                     GWASPQEIDARRLRIGVPVGQSITIANEGSLPATYRLNARTAGDHGLVAQLSVIATRS
                     ADGATIFSGPVTRLRSVDLGRFRAGEQETLQLRVLLTSTGTDAQDNGLQRRAATVAFT
                     WTATQA"
     gene            complement(725..2566)
                     /gene="nhaA"
                     /locus_tag="VF225_05620"
     CDS             complement(725..2566)
                     /gene="nhaA"
                     /locus_tag="VF225_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX7144770.1"
                     /translation="MQTPLREFLRTETGGAAVLLAAAAAALVWVNVDASSYDSLWRTK
                     LSIDIGDAGVALDLRHWVNSGLMTFFFFVVGLEARREFDLGELRERRRFALPLVAGIG
                     GMALAIAIYLAFNAGRSSAHGWGIAMSTDTAFALGLLALVGPRFPDRLRAFLLTVLVV
                     DDVVALVVIATVYTKTLHVVPLLAAVGFLGVVLVVRSAGVRTGLAYAALGAAAWVSLL
                     KSGIEPVVIGLAMGLLAYAYPAPRSSLERASERFREFREQPTAELARVAGVEIRAATS
                     SNERLQQLFHPWTSYVIVPLFALANSGIVVSGSFLAQAFRSPITLGILVGYVVGKPVG
                     ILGGSWLVTRLSHRRLQPPVGWAAVAGGGTIAGIGFTIALLIATLAFDSRQLEEAKVG
                     VLSAALSAALITWLLFRATALLPRRLRIRALLGSAQPLVDLYRDVDPERDHIRGPIDA
                     PVTVVEYGDFECPYCGQAEPVVRELRRDFTDVRYVWRHVPLNDVHPHAQLAAEAAEAA
                     GDQGAFWEMHDLLLDNQDALQPDDLVSYAELLGLDVDRFTNSLREHTGAGRVAEDIDS
                     ADLSGVTGTPTFFINSRRQHGAYDIASLSAAVRAAGARATLTSTSAG"
     gene            complement(2801..3073)
                     /locus_tag="VF225_05625"
     CDS             complement(2801..3073)
                     /locus_tag="VF225_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144771.1"
                     /translation="MESIICPVCGFIAETTDASRLRTVWADHLLGGCGDDVSAITTAM
                     ESCIEQRQELWRSGMTDEVPGLTVTIDLLWEALRYARLAEPPAAAA"
     gene            3168..>4256
                     /locus_tag="VF225_05630"
     CDS             3168..>4256
                     /locus_tag="VF225_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144772.1"
                     /translation="MFYLRYLRSELVGRRTRTVLTLVGLALGVALVITISGLARGLDR
                     AQKTALNPLSAIGTDLTVTLSPQQDTGGFGRGGGGEVVQANQSAVTDLSKLGKPGTQF
                     VHDFFLPGTQLTFPQQQTAKIASLPGVASISNGLVLSAVHQEGVVPKIVAKIKAGGQQ
                     LDIRRQIKPPTAAEAAAIQACFQKLGVGGGPQSGTQPGGQQGFGGRGAGGGGFFSSPA
                     ARKCLPARMREFRARITTPQQTLKQVVSPPQTNIKSSSYTIGGIDPAQQSIGLVTPSQ
                     LSRGRFFSKSGGSEALVSAAYAKRKSLKLGSTLDLNGTRFKIIGLVSPPLGGQTADVY
                     IPLAQLQKLASQKGLANVVLVRATSSSDV"
BASE COUNT          733 a         1418 c         1443 g          662 t
ORIGIN      
        1 tctgtctgcg tctatcccgg gtgagagggc tggctcatgc ggcaaagcgg gcccaggcgc
       61 gaggtcaagg cgcggcgaaa acctatcaga tttgttacgt gtcgcacaaa gcggtcaggc
      121 ctgagttgcg gtccaggtga acgcgacggt tgcggcgcgt cgctgcaagc cgttgtcctg
      181 cgcgtcggtg ccggtcgacg tcaacagcac gcgcagctga agcgtctctt gctctccggc
      241 tcggaaccgc ccgaggtcga cggagcgcag gcgtgtcacg gggccgctga agatcgttgc
      301 tccatcggcg ctgcgggtgg caatgacaga gagttgcgct acgaggccgt ggtcgccggc
      361 agtgcgtgcg ttcagccggt acgtcgcggg cagggagcct tcgttcgcaa tcgtgattga
      421 ctggccgacc gggacgccga ttcgtagccg tcgagcgtcg atctcttggg gggatgccca
      481 gccgccggtg accacggtcc catgcgtgaa cgacgcgccg aagacgctcg ggctgttgag
      541 cgccgccagc accgagggca cgacaacgag cgtcgcgagc gcggcgagaa gcgagacgaa
      601 gatcttccgg agcatgctct catcgtcgtc tcgctcggcc gcgccgtccc tccccgcggg
      661 ggagtcggtg gtgtccccga ttcgggttgg ccgggccact catcaggtgg ccgcggagga
      721 gtgctcaccc ggcgctcgtg ctcgtcaggg tcgctcgcgc tccggccgcc cgtaccgctg
      781 ccgatagcga ggcgatgtcg tacgcaccgt gctggcggcg actgttgatg aagaaggtgg
      841 gcgtgccggt gacgccgctg aggtctgcgc tgtcgatgtc ttcagcgacg cggcccgcgc
      901 cggtgtgctc gcgcaggctg ttcgtgaagc ggtcgacgtc gaggcccagc agttcggcgt
      961 aggagaccag gtcgtcgggc tggagcgcgt cctggttgtc gagcaggaga tcgtgcatct
     1021 cccagaaggc gccctggtcg cccgctgcct cggcggcttc cgcggccagt tgggcgtgag
     1081 ggtggacgtc gttcaggggc acgtgccgcc agacgtaacg gacatcggtg aagtcgcggc
     1141 ggagctcgcg gacgaccggt tccgcttggc cgcaataggg gcactcgaaa tctccgtact
     1201 cgacaaccgt gaccggcgca tcgatcggcc cgcgaatgtg atcgcgttcc ggatcgacgt
     1261 cccggtagag gtcgacgagc ggttgagcag acccgagcag cgcgcggatc cgcaggcgcc
     1321 gaggcaggag tgccgtcgcg cggaacagca gccaggtgat gagcgccgcg ctcagagcgg
     1381 cgctgaggac gccgaccttc gcctcttcga gctggcgact gtcgaaggcg agtgtcgcga
     1441 tcagcagcgc gattgtgaag ccgatccccg cgatcgtgcc gccgccggcg accgccgccc
     1501 agcccaccgg aggctgcagc cgtctgtggc tcagacgtgt caccagccac gagccgccga
     1561 gaatgcctac cggcttgccg acgacgtagc cgaccaggat cccaagcgtg atcggcgacc
     1621 tgaacgcttg tgccaaaaag ctgccgctga ccacgatccc agagttcgcg agtgcgaaca
     1681 gcggcacgat cacgtaactc gtccaggggt gaaacagttg ctgcagccgt tcgttggacg
     1741 acgtcgccgc cctgatctcc actccggcca cgcgcgccag ttcggcggtg ggctgctcgc
     1801 ggaactcgcg gaaccgttcc gaagcgcgct ccaaactgga tcgcggagcc gggtaggcgt
     1861 aggcgaggag ccccatcgcg agcccgatca cgacgggctc gatccccgac ttgagcaggc
     1921 tcacccacgc ggcagcaccg agcgctgcat aggcgagacc ggtgcggact ccggcgctac
     1981 gcacgacgag cacgactccg agaaagccga ctgctgcaag tagtggcacg acgtggagcg
     2041 tctttgtgta gacggtcgcg atgacgacga gcgcgacgac gtcgtcgacg accaagaccg
     2101 tcaggaggaa cgcccgcaga cggtcgggaa agcgcggccc gacgagagcg agcaggccga
     2161 gcgcgaatgc cgtgtcggtc gacatggcta ttccccagcc gtgcgcagac gagcggccgg
     2221 cgttgaaggc gaggtaaatc gcaatcgcca gcgccatgcc cccgatcccg gcgaccaggg
     2281 gcagggcgaa acgccgccgc tcgcgaagct ctcccagatc gaactctcgt cgcgcctcga
     2341 gcccgacgac gaagaagaag aacgtcatca gcccgctgtt cacccaatgg cgaagatcca
     2401 gcgcgacgcc tgcgtcgccg atgtcgatcg agagcttcgt cctccagagc gagtcgtacg
     2461 acgatgcgtc gacgttcacc cagacgagcg ccgcggccgc cgcggccagc aagaccgcgg
     2521 caccgccggt ctcggtacgc aggaactccc gcagcggcgt ctgcacgctg cgcgcccagg
     2581 ccgttcgtcc cgagaacgac ggtgacggcc gaccgcccgg atcgccgtca gtgctcgcgg
     2641 tgtcccgccc agacgacgtc ggcagagctc ttgatcgctg ccatctcaac cttctccctt
     2701 ttggaagcgt gcagcggccg cggccgcttc cgaacgattt ttgccgatat ccctcctcga
     2761 ccggaaacgc ggagcgtctg cggaggcttc ccgagcggcg ctaggccgct gccgcgggag
     2821 gctcggccaa gcgcgcatac cggagcgctt cccagagcag gtcgatcgtc accgtcaggc
     2881 cgggaacctc atccgtcatt cccgagcgcc agagctcctg cctctgttcg atgcaggact
     2941 ccatcgccgt cgtgatggcg gaaacgtcat cgccacagcc accgagcaga tgatcggccc
     3001 acacggtgcg gagccgagac gcatccgtgg tctcggcgat gaatccgcac accgggcaga
     3061 tgatgctctc catggtttgc atggtagacg cctagccagc cagctctggg agaaagtctt
     3121 actcaagact tagccgtgtc ttaggtcact gctgttagct gcgatcggtg ttctatctcc
     3181 gctacttgcg tagcgagctc gtcggccgca ggacgcgaac ggtcctcacg ctcgtcgggc
     3241 ttgctctcgg cgtcgcgctg gtgatcacga tcagcggcct cgcccgtggg ctcgaccgag
     3301 cccagaaaac cgcgctcaac ccgctctcgg cgatcggaac cgacctcacc gtgacgctgt
     3361 cgccgcaaca ggacaccggc ggctttggcc gcggaggcgg cggcgaggtt gttcaggcga
     3421 accagtctgc cgtcaccgac ctctcgaagc tcggcaagcc gggcacgcag ttcgtgcacg
     3481 atttcttcct gccggggacg caactgacat tcccgcagca gcagacggcg aagatcgcgt
     3541 cgcttccggg ggtcgcatcc atctcgaacg gcctcgtgct ctccgccgtc caccaggagg
     3601 gcgtcgtgcc gaagatcgtc gcgaagatca aggccggcgg acagcagctg gacattcggc
     3661 ggcagatcaa gccgccgacc gcagctgaag cggcagctat tcaagcgtgc ttccagaagc
     3721 tcggagtcgg cggcggcccg cagtccggca cgcaaccggg cggccagcag ggattcggcg
     3781 gcaggggcgc gggcggcggc ggtttcttca gctcgccggc cgcacgcaag tgcctgcctg
     3841 cgcggatgcg cgagttccgc gcccgaatca ccacgccgca gcagacgctg aagcaggtcg
     3901 tcagcccgcc gcagacgaac atcaagagct cctcgtacac gatcggcggc atcgatccgg
     3961 cacagcagtc gatcgggctc gtcacgccgt cgcagttgtc acggggccgc ttcttctcga
     4021 agtccggtgg aagtgaggcg ctcgtctcgg ccgcgtacgc gaagcgcaag tccctgaagc
     4081 tcggctcgac gctcgacctg aacggaacga ggttcaagat catcggactc gtaagtccgc
     4141 ccctcggcgg tcagactgcg gacgtctaca tcccgctcgc gcagttgcag aagctggcga
     4201 gccagaaggg gctcgcgaac gtcgtcctcg ttcgcgcgac gagcagctca gacgtc
//
LOCUS       DASVEU010000142        19873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1067693,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000142 DASVEU010000000
VERSION     DASVEU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 19873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19873
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067693"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="VF225_05635"
     CDS             <1..1610
                     /locus_tag="VF225_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7144773.1"
                     /translation="LERPEGEEVPSTRDVRSVAHMKMLQSLGGKLNRLNDVRRIGNVI
                     ATELRSLIDYHNCRVSVVEGNDVIPIAFVGQFTSETQEPLEILACKVGQGITGRVAET
                     GESLLIGDAANCEFARVLPGTDAIDESQVVVPLTYGTRVVGIIVISKLGLNQFDEDDV
                     RLMEVLAGHAAVALENAGLYEAARREAERATALLEFSRQLSTAEGMDEVVDRIVERSA
                     RTLGSPRASVWFQETTGGELRVHATHGYSELDHGRLARMHFDHESTEAFFSGKEPFVV
                     TDEQRRAIKGADQVGTGTPFAIAPLTLDGGRLGCIAVAAPDDGGFSERHLRLLAGVAH
                     QAKLALTNAGNFQSLEKTFLETVEALANALEANDEYTSSHARWITDLALKVGEGLGLD
                     TRSLKRLELGALFHDIGKIGIPEAILSKPGPLTAEERELVEKHPELGERIIAPIDRLE
                     EVRPIVRHCHERYDGTGYPDGKGGEEIPIESRIILVCDAYHAMTTDRPYRKRLPQEEA
                     LRRLDEGAGTQFDPAVVDVCKRVLAES"
     gene            complement(1623..2729)
                     /locus_tag="VF225_05640"
     CDS             complement(1623..2729)
                     /locus_tag="VF225_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144774.1"
                     /translation="MESPGDSWESVGWPEEDADRDQVLRLARQLAEARERQNVEALLQ
                     VEDLKRALRERAADVARRELEVERRAREMEELDTRGHEGRRLRLRRSERSQEADDGYA
                     EELVRRREQEAQQRLDALVPREREAAKLETALRARELEVEKAESAVASREQEVTVSKA
                     SAERQRLELESSYARLRETEAAFAERRAVLEEQERLLQASRMEISRMTDEHVARVASL
                     EAAEQELEAERSRLAAASGHERGADEREQRFAARARELEERAKAVADRELRLAARKRE
                     APVVEERSTAEGERKLAQRESQLAAREAALLAREAELLRVQSGLANQQEGIRRRERAL
                     EDAERVRERETALPAAPYVSFTEGLDAFSGGRQR"
     gene            complement(2829..5357)
                     /locus_tag="VF225_05645"
     CDS             complement(2829..5357)
                     /locus_tag="VF225_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144775.1"
                     /translation="MGRRGLPVTLAALAVSAVLGALGPSAASASWDQVRVKVVTFHYK
                     THDGHRRAAYVVLPDWYGPGNNPPLPLIISPHGRGVSAEENVDRWGNLPTLGPFAVVN
                     PQGQGRRFQRFSWGYPGQIEDLARMPQLVRRALPWLQIAPHRVYAFGTSMGGQETLLL
                     VARHPKLLAGAAAFDPVTDMARRYRDFARLPCNGGCLRKWGDPLGFGLRKLAREEIGG
                     TPLSHPRAYARRSPITYARAIASSGVPLQLWWSRTDRVVRSADQSVPLARRLRRLNPV
                     APIQTVAGSWPHSADMRPYFMLIPALQKFALVSTRNPSSLKTAQSAPVHPLLPWHGAV
                     RDAQGRLLPWYRPQVGLGYDHVLRIGWKFIEQGVPRDPRTGAKVYLHYAVFNGRTLHG
                     AYWQHNPAFLNATFVDSLVSWYPYSGDRRAVQAVREMLDYQLARGTSPTGWAWPRVPF
                     ATSCAGERRYGRCLAGLPRRFYGGIETDKVGLLGLGYVLFYELTGGRRYLNAAVAAGN
                     ALARHVRPGDATHTPWPFRVNARTGAVLDGAQFGGLVVGPVRLLDELIELGAGTTGAY
                     TRARDLAWSWLLKHQLNPDSPAWNRWSGFYEDVPYNPRSLNQAAPTLTAQYLLSQNSP
                     EALDPLWDEHVKTVLRWVRTSFGRGPFAGAWGIDEQRAPGRPGCCSRVGLGSTTSRWA
                     AANALLYARTGDETAREQAVRSLSYATYFATRDGRISCCGQRPGNTFWFSDGYGDYLR
                     SFNWAMAAMPELAPKRQNHLLGSGSVVQSVAYSRSSVAYRTFDAASTEVLRLRFRPRR
                     VTAGTTLLEREDLGAEGYVIQPLGGGDYTIRIRHDRSRRVRIDG"
     gene            complement(5419..6759)
                     /locus_tag="VF225_05650"
     CDS             complement(5419..6759)
                     /locus_tag="VF225_05650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7144776.1"
                     /translation="MESRFRSRYGRLAVTRAALVVFLLFATAIAAVVFTLDLSARHSN
                     RQEAGTELASTARVAASTFSTMRANLRTRVSELAASSALQQAVLGKNTAQLKSLAASH
                     DARIELRGRGFGKLPQKPRVMATATLARRSHAVARVTMAVPMDRSLLAVLQSATPMPK
                     HAALVLVQNGRVIAGGPAGTHVDVAARRIELGGVPFFSTTTKLVAGNASLAAVEPVSA
                     VAARVNAYRRRLLFAAAITLALAAGLATRLARPVARVFADLARLTRQAQTDSLTNLAN
                     RRALDERLDDEVDHARRLGTNVAFVIADIDNFKSINDTYGHQTGDEVLRRVARTFADA
                     VRELDLPGRYGGEEIALVLPGTNLTGARALSEKIRKGLEELAHATPEGIPIQVTASFG
                     AACFPAQSNAEDLVAAADAALYEAKRTGKNRVVTATAKRKPRGQATAADAPAPA"
     gene            complement(6760..7695)
                     /locus_tag="VF225_05655"
     CDS             complement(6760..7695)
                     /locus_tag="VF225_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144777.1"
                     /translation="MASRLLSTFESAGLAATATFAGVLWVGLALPGLPLLPGVGGGQG
                     SNVAISLNSAVLGVQDSRTFASSDGIAGRQARLLSLLLPTPDPLAEELATKRRGAAPT
                     SGVPIVVALPAVHTAAPRSDPRVVESSQLASTAYTPAPAPAPAPPAPPPPPRPPAPSP
                     PAPPRTPDPDPETPAPPTGPSAPPLPDADLAAMAEDTPPSDPSSPGNAPGSGPGSGNG
                     NAGGNNPNAGGANAGGNGNGNAGGNNPNAGGNNPNAGGANAGGNGNGNAGGNNPNAGG
                     NNPNAGGGNPNAGGANAGGNGNGNGAPKGSPKKGP"
     gene            7890..10289
                     /locus_tag="VF225_05660"
     CDS             7890..10289
                     /locus_tag="VF225_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7144778.1"
                     /translation="MKSAARAVVIGGGVGGCSILYWLTRLGWGDVVLCERAGLTSGST
                     FHSAGLVGQLRGSLSLTRMMMSSVELYRTLGDEVELETGWHEVGSLRLASSQERMEEL
                     ARQAGWAKTFGLPLQLVSAADAQGLFPPMSTEGVLGAAYLPSDGYIDPSQLTFALAEG
                     ARRRGAEIYTNTRVTAIAVDRNRVTGVVTDQGEIQAEIVVDAGGIFAGEIGRLAGVNV
                     PVVPMAHEYLITKPHDVPLDVPTMRDPSLLVYFRGESGGLVMGGYERHPAPWSLDGIP
                     ADFNGKLLAEDWPRFEELMEGALVRVPELGDAEVIKLINGPEAFTPDGEFILGPSDVR
                     GFWIAAGFCAHGLAGAGGIGQLLAEWIVEGLPSLDVWEMDSRRFGRHYESREYTLART
                     TEVYSTYYDVKYPGHERSAGRPLRVSPTYARAQELGAVFGEKSGWERVNWFEPNAALG
                     DESLRPRGWAGRVWSPAIGAEHVACRTTAALFDETSFAKIEVVGEEAADFLERLSDNK
                     VARDVGAITYTQMLNQRGGIECDFTVTRLADDRFRIVTGTAFGRHDLAWIRLHAPDGV
                     QVEDVTSRYACLGLWGPAARAILQPLTTEELAFPYMRARELAVGSVPCLALRVTYVGE
                     LGWELYCPVEYGLRLWDTISEGGREHGLVAGGYKAIDSLRLEKGYRVWGSDISPEDTP
                     FEAGLGFAVKLEKGNFIGRDALLTANEPTRRLACLVLDDPRSVALGSEPVRVGGEIVG
                     RVTSGGYGYTVGRSIAYAYLPSSSAAPGTRVEVEIFGEWIQGTAAEEPLFDAKGERIR
                     A"
     gene            10312..11157
                     /locus_tag="VF225_05665"
     CDS             10312..11157
                     /locus_tag="VF225_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEX7144779.1"
                     /translation="MEPEQLAERVWPGKDVAIEPLGGGITNRNFKVETEGRRFVLRIG
                     GKDTDLLGIDRAAEHAASRIAADLGLGPEVVAFVEPEGYLVTGYVDGEVGKVDVARVG
                     AALRLLHDGPAFPGRFDSFRVVEAYRATAAEHGVGVPPAFDRAQELANQIEQRRSHAP
                     VRPCHNDLLNANFIDDGVRLVLVDWEYAGMGDPFFDLGNFAVNHELTEESEQALLLAY
                     GSDDADALVLMRFMSDFREAMWGIVQLAISELDFDFAAYAEEHFERLARTAADPRFLS
                     ALQAP"
     gene            11233..12828
                     /locus_tag="VF225_05670"
     CDS             11233..12828
                     /locus_tag="VF225_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7144780.1"
                     /translation="MSKSAATPTLEGTRMVDIHDETPGEAARDEADLARFGYKQELRR
                     TLGVFSSFAVAFSYISPSTGIFALFFLGLTTIGGVFFWSWPIVALGQFIIALTFAELS
                     SHYPVAGSVFQWTKYLSGKTYSWFAGWIYLFAGILTVSSVCVTLPLALIPAFNGMGWD
                     VSSSLHNQRIVAIVTLAGITILNIFGVRLVALINNTGVIFEILGMVVFAFILAIFHNH
                     QGVGVIFHTGGQSLTVSTFLVAMFMSLYVIYGFDTASTLAEETRDPRREAPKAVLASV
                     VGAFVVGAVFLWATLMAVPDMGAAIKGAFGPAQIIDANFSNAFATVYLLVVAAAIFVC
                     CLAIQASTIRLCFGMSRDEQLPGSSLLRVVHPRLHTPIGACLAVGIFAFIPMLQYTGA
                     AIVAIAATGMIYLSYLICNLAVIRARLRGWPRTAAPFSLGKWGIPITAVGLLWGGGML
                     VNFAWPRVATNPTPNQTGKLLNFHWHWLNDQPILWTVAIVIALVGAVYFILVQRTKPA
                     HLQAPEGEVFAEEMPRPAAAPSA"
     gene            12960..13181
                     /locus_tag="VF225_05675"
     CDS             12960..13181
                     /locus_tag="VF225_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144781.1"
                     /translation="MEQVVDLATRKTGSIGVALVWDRNDKTLRVFAYDGLTGEEIVVP
                     VSGAEAAEVYRHPFAYAARSIKPKLPAGD"
     gene            complement(13404..14348)
                     /locus_tag="VF225_05680"
     CDS             complement(13404..14348)
                     /locus_tag="VF225_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX7144782.1"
                     /translation="MTTTQYLNQVRARFIRVLAALAVLICTSFGATSTQSAAAASGRT
                     VTNNAIELENLRHAIASSSNWAGYAVTPLDATTSTIPPTPPAPPAPTTPTSFTSVSGS
                     WVQPKVSCTKSKATYAAFWVGLGGFSPTSQALEQIGTQVTCTTAGKAKYSMWYELVPA
                     ASVPIRFKVFPGNAFTASVKVDGTQVTLEIRNLTRRTNFTKTLFMAEPDVSSAEWIAE
                     APTGCNASRCVQLPLAKFSKLTFTKASATTSDGHTGTISDVSWSPTVISLENSASDPF
                     ATDTSGALPSPLSTNGASFAVVWQQAISTAARSRASRN"
     gene            complement(14361..15482)
                     /locus_tag="VF225_05685"
     CDS             complement(14361..15482)
                     /locus_tag="VF225_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7144783.1"
                     /translation="MQTRTLSSAILTTAIDPLEVPMLNPILAWGRYRLLLGPLVAAVV
                     AGGIVVAAALAMGSSSSSSSTNTVDSGTVTPGLALQGAIVKVVQSVSPSVVQIQDQVG
                     LGSGIVLDAAGYIVTNNHVVTGAKSLTVTTSNGSQYPATLVGTFPSDDLAVIKISGAD
                     LKAATFGDSSKLNVGDLAIAIGNPLGLRSSVTDGIVSAFRQDVSEGRNGVTLPSVIQT
                     SAAINPGNSGGALVDIQGRVIGIPTLAATDPELGGSAPGIGFAIPGNLAKDIARQIVT
                     NGKVVNYHRAYLGLKLGETSGKGLYVGSVSAGGPAAKAGIDAGDVIVSVDGHATSTIN
                     VLSSVLAGLKPGQEVSVVVKNQDGRKATLQVTLGTYPGS"
     gene            complement(15637..15837)
                     /locus_tag="VF225_05690"
     CDS             complement(15637..15837)
                     /locus_tag="VF225_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144784.1"
                     /translation="MEPMSMPTAEQDDEELLVHEWRAEQLRRLGVRRTFAEAFAGIVD
                     WHEIADLVARGCSPELALEIVR"
     gene            16006..16377
                     /locus_tag="VF225_05695"
     CDS             16006..16377
                     /locus_tag="VF225_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144785.1"
                     /translation="MAISVLIVDDNGSFLDAARVLLEREGLSVVGVASTGAEALRRAE
                     ELSPDVLLVDITLGRESGFELTRRLVEDGRGKRSAVILISTHSEADFADLIAESQAIG
                     FVPKSELSAEAIRRILDRGSR"
     gene            16395..17909
                     /locus_tag="VF225_05700"
     CDS             16395..17909
                     /locus_tag="VF225_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX7144786.1"
                     /translation="MSALRRLVGASLRQRSPARARRLLRLPRRHRAAARVLAVVGPVV
                     VAVGLLPFRSSLGLAGVLFCTLLAVVAVALIGGVGAALTAVVVGFLAGALLFAHPYGS
                     LRPDSPVDVVALIAFVVVGGVIGILVDDLTRLGEEQAALRRVATLVARTAPADEVFAA
                     VTEEAGQLLPVDRTSLSRYESDGTVTVVASWSRDGGHVPVGTRTMLGGKNLSTFVSET
                     SRPARMDSYADISGALGAAIRETGPRSGVGTPVIVAGRLWGVLIAASTSERRLPSDLE
                     ARLADFTDIAATAVANAENRADLAASRARIVATADETRRRIERDLHDGAQQRLVSLAL
                     ELRAVQAMLPPEFGELDGELSRVAEGLTSVLDELRETARGIHPAILAEGGLGPALKTL
                     ARRSAVPVELDVRVDARLPERIEVATYYIVSETLTNAAKHAHASIVRVGVEAVDGVVR
                     VSVRDDGAGGADSARGTGLIGLRDRVEALGGAIAVESPLGAGTAVRIELPTASS"
     gene            17998..18207
                     /locus_tag="VF225_05705"
     CDS             17998..18207
                     /locus_tag="VF225_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144787.1"
                     /translation="MTLSHAQALFREAVHDLAGEPTPANVQRYLAASQILDRAEPAEA
                     ATNSRSNGAATGEDQPAGALALERS"
     gene            18210..19121
                     /locus_tag="VF225_05710"
     CDS             18210..19121
                     /locus_tag="VF225_05710"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7144788.1"
                     /translation="MRGEPAMATRIGFIGLGRMGGNMAARLLAAGYPIYGEEQTRADA
                     QGLIQRGLRWRDTPRGVAEAADVVFTSLPDDEVLEVVASGPDGILAGLAAGKIWVDMS
                     TVSPRASRELAARVRELGASMLDAPVSGSVPQVQAGTLTIMVGGDEQAYAQVEPILRE
                     LGTPTHIGENGQGLVLKLAINISLAVQMLAFAEGLVLADRAGIDRELAVGVMTQSAIG
                     SPMLKARAELVLDLPEDAWFDVSLMQKDVVLALDAARELHIPLPSTAAADEVLTVARA
                     LGYEHRDLAAMFEVLGQLAGGPALSQA"
     gene            19118..19762
                     /locus_tag="VF225_05715"
     CDS             19118..19762
                     /locus_tag="VF225_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144789.1"
                     /translation="MTDQIRRAGPPGNERLTTSVGLVLLVLLIAETLTTLSARSLLPV
                     HLFLGLLLLPPVALKLASTGWRFLRYYAGDRLYRQEGPPRLLLRLLAPVLVASTLVLF
                     GSGIALAVVGHGGVWFAVHIVSFGVWGLLMIVHVLVYLTRTLRDGTADWHRRVERVVV
                     GARRRRAALGGAVLAGVILAVATYPAQQGWVSHRGDRHRDGLGSAPTFALRVND"
BASE COUNT         3054 a         6565 c         6856 g         3398 t
ORIGIN      
        1 agctcgagcg tcccgagggc gaagaggtcc cgtctacgcg cgacgtgcgc tccgttgcgc
       61 acatgaagat gctgcagagc ctcggcggca agctgaaccg tctcaacgac gtgcgccgaa
      121 tcggcaacgt gatcgcgacc gagctgcgct cgctcatcga ctaccacaac tgccgagttt
      181 cggtggtcga gggcaacgac gtgattccga ttgcgttcgt cggccaattc acgtccgaga
      241 ctcaggagcc gctcgagatc ctcgcgtgca aggtcggcca gggcatcacc ggccgcgtcg
      301 ccgagacggg cgagtcgctg ctgatcggcg acgccgcgaa ctgcgagttc gcgcgcgtgc
      361 ttcccggcac cgacgccatc gacgagtccc aggtcgtggt cccactgacg tacgggacgc
      421 gcgtcgtcgg catcatcgtg atctcgaagc tcggcctgaa ccagttcgac gaggacgacg
      481 tccggctgat ggaggtgctc gccggccacg cggcggtggc actcgagaac gccgggctct
      541 acgaggctgc gcgccgggag gcggaacgcg ccacggcatt gctggagttc agccggcagc
      601 tttcgaccgc ggagggcatg gacgaggtcg tcgaccggat cgtcgagcgg tcggcgcgaa
      661 cgctcgggtc tcctcgtgcg tccgtgtggt tccaggaaac gaccggcgga gagctcaggg
      721 tccacgcgac ccacggctac tcggagctcg accacggacg cctcgcccgg atgcacttcg
      781 accacgagtc cacggaggcc ttcttctccg gaaaggagcc gttcgtcgtc accgatgagc
      841 agcgtcgcgc gatcaagggc gcggaccagg tcggaacggg gacgccgttc gccatcgcac
      901 cgctcacgct cgacgggggc cggctgggct gcatcgcggt cgcagcaccc gacgacggcg
      961 ggttcagcga acggcacctg cgcctgctcg caggcgttgc ccaccaggcc aagctggcgc
     1021 tcacgaacgc gggcaacttc caaagcctcg agaagacctt cctcgagacc gtcgaggctc
     1081 tcgcgaacgc gctcgaggcg aacgacgagt acacgtcctc acacgcgcgc tggatcaccg
     1141 atctggcgct gaaggtcggc gaagggctcg gcctcgacac tcgctccctc aagcggctgg
     1201 agctcggcgc gctcttccac gacatcggga agatcggcat cccggaggcg atcctctcga
     1261 agccgggccc gctgacggca gaagagcgcg agctggtcga gaagcatccc gagctcggcg
     1321 agcgaatcat cgcgccgatc gaccggctcg aggaggtccg gccgatcgtg cgccactgtc
     1381 acgagcgcta cgacggcacc ggctatcccg acggcaaggg cggcgaggag attccgatcg
     1441 agtcgcggat catcctggtc tgcgacgcct accacgcgat gaccacggac cggccttacc
     1501 gcaagcggct cccgcaggaa gaggcgctgc ggcggctcga cgaaggcgcc ggcacgcagt
     1561 tcgatcccgc agtagtcgat gtctgcaagc gcgtgctcgc ggagagctag ggccccgagg
     1621 ccttagcgct gccggccgcc gctgaaggcg tccagtccct cggtgaaact gacatacggg
     1681 gccgcgggca gcgcggtctc acgctcgcgc acacgctctg cgtcttcgag cgctcgctcg
     1741 cgccttcgaa tgccttcctg ctgattcgcc aggccggact gaacgcgcag gagctctgcc
     1801 tcgcgtgcga gtaatgccgc ctcgcgggcc gcgagctgcg actcgcgctg cgcgagcttg
     1861 cgctcgcctt ccgccgtcga tcgctcctcg acgacagggg cttctcgttt gcgggcggcg
     1921 agccgcagct cgcggtcggc gaccgccttc gcgcgctcct cgagctctct cgcccgagcg
     1981 gcaaaccgct gctctcgctc gtcggcgccg cgctcatgtc cggaggcggc tgcaagccgg
     2041 ctgcgttctg cctccaattc ttgctcggcg gcttcaaggg acgcgacgcg cgcgacatgc
     2101 tcgtctgtca ttcgcgagat ctccatgcgc gacgcttgga gcagtcgttc ctgctcctcg
     2161 agcacggccc gtcgttcggc gaacgcggcc tcggtctcgc gcaaccgcgc gtacgagctc
     2221 tcgagctcga gcctctgtcg ctcggccgag gccttcgaca ctgtgacttc ctgctcgcga
     2281 ctcgccaccg cgctttcggc cttttcgacc tcgagctcgc gcgcgcgcag cgccgtttcg
     2341 agcttggcag cctcgcgctc ccgcggaacc agggcgtcaa gccgctgctg agcctcctgc
     2401 tcgcgccgcc tgaccagctc ttccgcatag ccgtcgtccg cttcctgcga acgctcggag
     2461 cggcgcaggc gtagacgacg accctcgtgg ccgcgtgtgt cgagctcctc catctctcgg
     2521 gcgcgcctct ccacttcgag ctctcggcgc gcgacgtcgg ccgcccgctc gcggagtgcg
     2581 cgcttcagat cctcgacctg gagcagggcc tcgacgttct ggcgctctcg agcctcggcc
     2641 agttgccgcg caaggcgcag gacctgatcg cggtcggcgt cctcctcagg ccagccgacc
     2701 gattcccacg agtcccccgg cgactccatg ctctagagcg tagcgcgctt ccaggcgagt
     2761 ttgttcgctc cgagcaggga aaaccacgcg gagtgaaacg atgcccgtca gactgacgag
     2821 cgagcgagct aaccgtcgat ccggactcgc ctcgaacggt cgtgacggat ccggatcgtg
     2881 tagtcgccgc cgccgagtgg ctggatcacg taaccctccg cgccgagatc ctcgcgctcg
     2941 agcagtgtcg ttccggccgt cacccggcga ggccggaacc gcaggcgaag gacctcggtg
     3001 ctggccgcgt cgaatgtccg atacgcgacg ctgctgcgcg agtacgccac agactggacc
     3061 accgagccgg agccgagcag atggttctgc cgcttgggcg ccagctccgg catcgccgcc
     3121 atcgcccagt tgaagctgcg tagatagtcc ccgtagccgt cgctgaacca gaacgtgttg
     3181 ccgggccgct gtccacagca ggatatgcgt ccgtcgcgtg tggcgaagta cgtcgcgtag
     3241 ctcagcgagc gcaccgcttg ttcccgtgca gtctcgtcgc cggtgcgcgc gtacaacagt
     3301 gcgttcgccg ctgcccagcg agacgtcgtg ctgccgagcc cgacccgcga gcagcagccg
     3361 ggtcttcccg gcgcgcgttg ctcgtcgatc ccccacgcgc cggcgaacgg gcctcgcccg
     3421 aacgaggtgc gcacccagcg caggactgtc ttcacgtgtt cgtcccacag cgggtcgagg
     3481 gcctcgggcg agttctggct gaggagatac tgcgcggtca gggtcggcgc tgcctggttg
     3541 agactgcgcg ggttgtaggg aacgtcctcg tagaagccgc tccaacggtt ccaagcggga
     3601 ctgtcggggt tcaactggtg cttcaacaac caggaccagg cgaggtcgcg tgcgcgtgtg
     3661 tacgcaccag tggtcccggc tccgagctcg atcagttcgt cgagcaaccg tacgggcccg
     3721 acgacgagcc caccgaactg tgcgccgtcc agcacggcgc ctgtccgcgc attgacgcgg
     3781 aaaggccacg gcgtgtgcgt ggcgtcgccg gggcgaacgt ggcgcgcaag cgcattgcct
     3841 gcggcaacag ccgcattcag ataacggcgc ccgccggtga gctcgtaaaa gagaacgtaa
     3901 ccgagaccga gcagcccgac cttgtcggtc tcgatgccgc cgtagaagcg ccgggggaga
     3961 ccggcgaggc agcgtccgta gcggcgttcg cccgcgcagc tcgtcgcgaa cggaacccgc
     4021 ggccacgccc aacctgtcgg cgatgtgccc cgtgcgagtt ggtagtccag catttctcgg
     4081 accgcctgca ctgcgcggcg gtcaccggag tacgggtacc aggacacgag cgagtcgacg
     4141 aacgtcgcgt tgaggaacgc cgggttgtgc tgccagtacg caccgtgcag cgtccggccg
     4201 ttgaagaccg catagtggag atagaccttc gcgccggtgc gcggatcccg cgggacgccc
     4261 tgctcgatga acttccaccc gatgcgcagg acgtggtcgt agcctagccc gacctgcggc
     4321 cggtaccagg gcagtagcct tccctgcgca tcgcgcacag cgccatgcca gggcagtagt
     4381 gggtgaacgg gggctgactg cgcggtcttt agcgaggaag ggttgcgcgt gctcaccaga
     4441 gcgaacttct gcagcgcggg aatgagcatg aagtacgggc gcatgtccgc ggagtgcggc
     4501 cacgagcctg cgacggtctg gatgggcgcc acaggattca gccgtcgcaa cctgcgcgcg
     4561 aggggcaccg actggtccgc cgatctgacg acgcggtcgg ttcggctcca ccagagctgg
     4621 agtggcactc ccgagctcgc gattgcgcgc gcgtaggtga tcgggctgcg gcgtgcgtag
     4681 gcgcgcgggt ggctgagcgg ggtgccgccg atctcctccc gggcgagctt gcgcagcccg
     4741 aaaccgagcg ggtctcccca tttcctgagg cagccaccgt tgcacgggag gcgagcgaag
     4801 tcgcggtagc gtcgggccat gtccgtcact gggtcgaacg cggcggcgcc ggcgagaagc
     4861 ttgggatggc gtgcgacgag cagcaacgtc tcctgtccgc ccatgctcgt gccgaacgca
     4921 tagacgcggt gaggtgcgat ctgtaaccac ggaagcgcgc ggcggacgag ttgcggcatg
     4981 cgtgcgaggt cctcgatctg tccgggatag ccccaggaga agcgttggaa cctccggccc
     5041 tgcccctgcg ggttgacgac ggcgaacggg cctagtgtcg gcagattgcc ccagcggtcg
     5101 acgttctcct cggccgagac gccgcgtccg tgcggcgaga tgatgagcgg aagcggcgga
     5161 ttgttgccgg gcccatacca gtccggcagt acgacgtagg ccgcgcggcg gtgaccgtcg
     5221 tgcgtcttgt aatggaacgt cacgaccttc acccggacct gatcccagga tgcgctggct
     5281 gctgacgggc cgagcgctcc gagcacggcg gagaccgcga gggcggcgag agtgacaggc
     5341 agaccccggc gccccatcgg gagaatgatt ccacagcctc cggcggccgc gggttttgtc
     5401 gcgtcgcttt agacgaacct aggctggtgc cggtgcatcg gccgcagtcg cctggccgcg
     5461 gggcttcctc ttcgccgtcg ccgtgacgac gcggttcttg cccgttcgct ttgcctcgta
     5521 gagtgcggca tcggctgcgg cgactagatc ctcggcgttc gactgggccg ggaagcaggc
     5581 cgcgccgaag ctcgcagtga cctggatagg aatgccctcc ggcgtcgcgt gggccagttc
     5641 ctcgaggccc ttgcggatct tctccgagag cgctcgcgct cccgtcagat tggttccggg
     5701 aaggacgagg gcgatctctt cgccgccgta gcggccgggg agatccagct cgcggacggc
     5761 gtcggcgaac gttcgcgcaa cgcgccgcaa aacctcgtcc ccggtctggt ggccgtaggt
     5821 gtcgttgatc gacttgaagt tgtcgatgtc cgcgatcacg aaagccacgt tggtcccgag
     5881 tcgccgggca tggtcgacct cgtcgtccag acgctcgtcc agcgcgcgcc gattggcgag
     5941 attcgtgagg ctgtcggtct gtgcttgccg cgtcagccgc gcgagatcgg cgaacacgcg
     6001 ggcgacgggg cgcgcgaggc gcgttgcgag cccggctgca agcgcgaggg tgatcgccgc
     6061 cgcgaaaagc agacgtctcc gataggcatt cactcgagca gcgacggctg agaccggctc
     6121 gaccgcggcg agcgacgcat tcccggccac gagcttggtg gtcgtcgaga agaacggaac
     6181 acctccgagc tcgatgcgcc gagcggccac gtcgacgtgg gtgccggcgg ggcctccggc
     6241 gatcacgcgg ccgttctgaa ccagaacgag cgctgcgtgc ttcggcatcg gcgtcgcgct
     6301 ctgcaagacg gcgagcaggg aacgatccat cgggacggcc atggtcaccc gtgccacggc
     6361 gtgactacgc cgtgccagag ttgccgtcgc catgactcgc ggcttctgcg gaagctttcc
     6421 gaagccgcga ccccgcagtt cgatgcgcgc atcgtgtgag gcggcgagcg acttcagttg
     6481 agcggtgttc ttccccagca cggcctgctg aagtgccgag gacgccgcca gctcgctgac
     6541 ccgggtgcgg aggttggccc gcatggtcga gaaggtcgaa gcggcgactc gcgccgtgct
     6601 cgcaagctcc gtcccggctt cctggcggtt cgaatgtctt gcgctcaggt cgagcgtgaa
     6661 gacgaccgcg gcaatggcag tcgcgaagag aaggaagacg acgagcgcag cccgggtgac
     6721 ggccagccgg ccatagcgag aacgaaagcg gctttccact tacggtccct tcttcggcga
     6781 ccctttcggg gctccgtttc cgttgccgtt gccgcctgcg ttggcgcctc cggcgttcgg
     6841 gttgcccccg ccggcgttgg gattgtttcc gccggcgttg ggattgtttc cgccggcgtt
     6901 gccgttgccg ttgccacctg cgttggcgcc gccggcgttg ggattgtttc cgcccgcgtt
     6961 cgggttgttt ccgcccgcgt tgccgttgcc gttgccacct gcgttggcgc caccggcgtt
     7021 gggattgttt ccgcccgcgt tcccgttccc gctccccggg ccactacccg gagcgttacc
     7081 cggcgagctt gggtcgctcg gcggcgtgtc ctcggccatg gcggcaagat cggcgtccgg
     7141 aagcggagga gcagacgggc ccgtcggggg cgcaggtgtc tcggggtctg gatcgggcgt
     7201 gcgagggggc gccggcggcg atggggcagg aggccgcggg ggcggagggg gtgcaggcgg
     7261 tgccggcgcc ggcgccggtg ccggcgtgta tgccgtcgag gcaagctgtg agctctcgac
     7321 gacgcgagga tcgctgcgag gagctgcagt gtggacagcg ggaagcgcga ccacgatcgg
     7381 cacacccgaa gtcggcgctg cgcctcgtcg cttggtcgcg agctcctcgg cgagcgggtc
     7441 gggtgtggga agcaacaggg agagaaggcg cgcctgacgg ccggcgatcc catcggagga
     7501 tgcgaacgtg cggctgtcct gtaccccgag gacagcgctg ttgagagaga tcgcaacatt
     7561 gctgccctgg ccaccgccca cacctggcag caggggcaga cccggcaagg cgagcccgac
     7621 ccagagcacg cccgcgaacg tcgccgtcgc ggcgaggccc gccgactcga aggtcgacag
     7681 gagcctcgaa gccatcgagg gccgtgtggg tgttcgccgc ttgttctcca tcatcgccac
     7741 agcaagtact agtccgcagg tactagtcct gaacatcgac catttgccgt tcggagggcg
     7801 tcccccatcc gagtgagtgg ggctaagtag aaccgctgag gaccggcgag cgccacggcg
     7861 gatggacggg accgggatag ggtctgcccg tgaagagcgc cgcgcgtgcg gtcgtgatcg
     7921 gcggcggtgt cggcggctgc tcgatcctct attggctcac gcgacttggg tggggagacg
     7981 tcgtgctgtg cgagcgcgcc gggctcacga gcggctcgac gttccattcg gccgggctgg
     8041 tgggccagct ccgtggctcg ctcagcctga cgaggatgat gatgagctcg gtcgagctct
     8101 accgcacgct cggagacgag gtcgagctcg agaccggctg gcacgaagtg ggttcactgc
     8161 gccttgcgtc gtcgcaggag aggatggagg agctcgcccg ccaggccggt tgggcaaaaa
     8221 cctttggcct gccacttcaa ctcgtttcgg ccgcagacgc gcagggactc tttccgccga
     8281 tgtcgacaga gggcgtcctc ggcgcggcct atctgccgag tgacggttac atcgacccga
     8341 gccagctgac gttcgcgctc gccgaaggtg cgcgccggcg cggcgccgag atctacacga
     8401 acacgcgtgt cacggcgatc gccgtcgacc gcaaccgggt cacgggcgtc gtcaccgatc
     8461 agggcgagat ccaggcagag atcgtggtcg acgccggtgg aatcttcgcg ggtgagatag
     8521 gacgccttgc cggtgtgaac gtccctgtgg tcccgatggc gcacgagtac ctgatcacga
     8581 aaccgcatga cgtcccgctg gacgtgccga cgatgcgcga tccgtcgctg ctcgtgtact
     8641 ttcgcggcga gtcgggcggc ctcgtcatgg gcgggtacga gcgccacccg gcgccgtggt
     8701 ccctggacgg catcccggct gacttcaacg gcaagctgct cgccgaggac tggccgcgct
     8761 tcgaggagtt gatggaagga gcactcgtgc gcgtgcccga gctaggggac gcggaggtga
     8821 tcaagctgat caacggtccc gaggcgttca cgcccgacgg cgagttcatc ctcggcccgt
     8881 ccgacgtgcg tggcttctgg attgcggccg gcttctgcgc ccacgggctc gcgggcgccg
     8941 gcggcatagg gcagctgctc gccgagtgga tcgtcgaggg cctgccgagc ctcgacgtct
     9001 gggagatgga ctcgcgccgc ttcggccgcc attacgagag ccgcgagtac acgctcgcgc
     9061 gcacgaccga ggtctactcg acctactacg acgtcaagta tccggggcac gaacgcagcg
     9121 cgggacgacc tctgcgcgtc tcgccgacct acgcacgcgc gcaggagctc ggagccgtct
     9181 tcggagagaa gtcgggctgg gaacgcgtga actggttcga gccgaacgcg gctctcggag
     9241 acgagtcgct gagaccacgc ggctgggccg gtcgggtctg gtcaccggcg atcggagccg
     9301 agcacgtcgc ttgccgtacg actgccgctc tcttcgacga gacctcgttc gcgaagatcg
     9361 aggtcgtggg ggaggaagca gctgacttcc tcgagcggct gtctgacaac aaagtcgcgc
     9421 gcgacgtcgg ggcgatcacc tacacgcaga tgctcaacca gcgcggggga atcgagtgcg
     9481 atttcaccgt cacgagactg gccgacgacc gcttccgcat cgtcaccggg acagcattcg
     9541 gccggcacga tctcgcctgg atccgcctgc acgcgcccga cggcgtgcag gtcgaggacg
     9601 tgacctcgag gtacgcgtgc cttgggctct gggggcccgc agcccgggcg atcctccagc
     9661 cgctgacgac ggaggagctt gcgttccctt acatgcgggc acgcgagttg gccgttggtt
     9721 cggtgccgtg cctcgcgctg cgggtcacct acgtgggcga gctcggctgg gagctctatt
     9781 gcccggtcga atatggcctc cgcctctggg acacgatctc ggaaggcggt cgcgagcatg
     9841 gtctcgtcgc gggcgggtac aaggcgatcg actcgctccg gctcgagaag ggctaccgcg
     9901 tgtggggctc cgacatcagc cccgaggaca cgcccttcga ggcgggcctc ggtttcgcgg
     9961 tcaagcttga aaagggcaac ttcatcgggc gggacgcttt gctcactgcg aacgagccca
    10021 cccgccggct cgcctgcctg gtgctcgacg atccacgctc ggtcgcgctc gggtccgagc
    10081 cggttcgcgt gggcggcgag atcgtgggcc gcgtcacgag cggcggctac ggctacacgg
    10141 tcggacgctc gattgcctac gcctacctgc cgtcgtcgtc ggcggccccc ggcacgagag
    10201 tcgaggtgga gatcttcggc gagtggatcc agggcacggc cgccgaagag ccgctcttcg
    10261 acgcgaaggg cgagcgcatc cgcgcttaga gtcgcggtct aggatcgggc gatggagccc
    10321 gagcagctgg ccgaacgggt ttggccgggg aaggatgtgg cgatcgagcc gctcggcggc
    10381 gggatcacga accgcaactt caaggtcgag accgaaggac ggaggttcgt cctgcggatc
    10441 ggcggtaagg acaccgacct cctcgggatc gaccgtgcgg ccgagcacgc cgcctcgcgc
    10501 attgcggccg acctcgggct cgggcccgag gtcgtcgcgt tcgtcgagcc cgagggctac
    10561 ctcgtcacag ggtatgtcga cggtgaggtc ggcaaggtcg acgtcgctcg ggtcggcgca
    10621 gcgctccgtc tgctccatga cggccccgcc tttccggggc gcttcgacag ctttcgtgtc
    10681 gtcgaggcct accgcgccac cgccgccgag cacggcgtcg gggtgccgcc ggcgttcgac
    10741 cgtgcgcaag agctcgcgaa ccagatcgag caacgccgct cccacgcgcc cgtgcggccc
    10801 tgccacaacg acctgctgaa cgcgaacttc atcgacgacg gtgtccgcct cgtgctcgtc
    10861 gactgggaat acgccggcat gggcgacccg ttcttcgacc tcggaaactt cgccgtcaac
    10921 cacgagctca cggaggagtc cgagcaagcg ttgctcttgg cttacggcag cgacgatgcg
    10981 gatgcgctcg tcctgatgag gttcatgtcc gactttcgcg aggcgatgtg gggcatcgtg
    11041 cagctcgcga tctcggagct ggacttcgac tttgccgcct acgccgagga gcacttcgag
    11101 cggctcgccc ggaccgccgc cgacccgcgt ttcctcagtg cgcttcaggc gccgtaaacg
    11161 acctcgcgcg cctcggtgac acaagtgcgc aagctcgagt caccgacctc gattgacacg
    11221 atgcctggcg ccgtgtctaa atcggccgcg acccctaccc tggagggcac ccgcatggtg
    11281 gacatccacg acgagacgcc cggcgaggcg gctcgcgacg aggccgatct cgcccgcttc
    11341 ggctacaagc aggagctccg ccgcacgctg ggcgttttca gctcgttcgc cgttgcattc
    11401 agctacatct cgccgtcgac cggcatcttc gcgctctttt tcctggggct gacgaccatc
    11461 ggcggcgtct tcttctggag ctggccgatc gtggcgctgg gccagttcat cattgctctg
    11521 accttcgcgg agctgtcgag ccactatccg gtcgcgggat cggtcttcca gtggaccaag
    11581 tacctgtccg ggaagacgta ctcctggttc gccggctgga tctacctgtt cgccggcatc
    11641 ctgaccgtct cgtccgtgtg cgtgacgctc cccctggcgc tcatccccgc gttcaacggc
    11701 atgggctggg acgtctcgag cagccttcac aaccaacgga tcgtcgcgat cgtcacgctc
    11761 gccgggatca cgatcttgaa catcttcggc gtgaggctcg tggcgctgat caacaacacc
    11821 ggcgtcatct tcgagatcct cgggatggtc gtcttcgcgt tcatcctcgc gatcttccac
    11881 aaccatcagg gcgtcggggt catcttccac accggcggcc agtcgctcac cgtcagcacg
    11941 ttcctcgtgg cgatgttcat gagcctctac gtgatctacg gcttcgacac cgcgtccacg
    12001 ctcgccgagg agacgagaga tccgcgacgc gaagcgccca aagcggttct tgcctcggtc
    12061 gtcggtgctt tcgtggtcgg agcggtcttc ctctgggcca cgttgatggc cgtccccgac
    12121 atgggcgccg cgatcaaggg cgcgttcggc cctgcgcaga tcatcgatgc gaacttctcg
    12181 aacgcgtttg cgaccgtgta cctgctcgtg gtcgcagcgg cgatcttcgt ctgctgtctc
    12241 gcgatccagg cgtcgacgat ccggctctgc ttcggcatgt cgcgtgacga gcaactccct
    12301 ggatcgagct tgcttcgggt ggtgcacccg cgcttgcaca ctccgatcgg ggcgtgtctc
    12361 gccgtcggta tcttcgcgtt catcccgatg ctgcagtaca ccggcgcggc gatcgtcgcg
    12421 atcgcggcga ccgggatgat ctatctcagc tacctcatct gcaatctcgc cgtcattcgt
    12481 gcgaggctgc gtggctggcc gcgaacggcg gcaccgttct cgctcggcaa gtgggggatc
    12541 ccgatcacgg ctgtcgggct cctgtggggc ggcggaatgc tcgtcaactt cgcgtggcca
    12601 cgcgtggcga cgaacccgac tccgaaccag acgggcaagc tgctcaactt ccactggcac
    12661 tggctgaacg accagccgat cctctggacg gttgcgatcg tcatcgcgct ggtcggcgcg
    12721 gtgtacttca tcctcgtcca gcggacgaag cctgcgcacc tccaggctcc ggagggagag
    12781 gtgttcgcag aggagatgcc gaggcccgct gcggcgccgt cggcttagct tcttgggtgc
    12841 ggcgccggcg gtgcgaaagc cgccggcgcc ccgatgctcg acgtcccacc agccgcgagt
    12901 ctaggcctgt cgcgcccttc ggatagcgcg tccagcttgg cgaggatcat gctgagttca
    12961 tggagcaggt ggtcgacctg gcgacgcgca agaccggctc gatcggggtc gccctcgtgt
    13021 gggacagaaa cgacaagacg ctacgtgtct tcgcctacga cggactgacc ggcgaggaga
    13081 tcgtcgtccc ggtcagcggc gccgaggcgg cagaggtgta tcggcatccg ttcgcctacg
    13141 ccgcccgctc gatcaagccg aaactcccgg cgggcgactg acgtggcgag ttgggactcg
    13201 gggccagatg ccgacgcgaa gagaaccggc ctgcgctgtc tggcctgcgg tcgacgtctg
    13261 gacgaggcgc tcgctgtcgt tggctccctc cggtgcctcg actgcaggga gtcgaacgcc
    13321 actctgagga gtcagttgat cgcgccggca gtagcgtctg agcagagcga aggccggaat
    13381 cacctccggc tctcgccacc ctcctagttc ctactcgcgc gagaccgcgc cgccgtcgag
    13441 atggcctgtt gccagacgac cgcgaaagat gcaccgttgg tcgatagcgg cgaaggaagc
    13501 gccccgctcg tgtcggttgc gaagggatcg cttgcgctgt tctccagact gatcacggtc
    13561 ggagaccagg agacatcgct gatcgtgccc gtgtgaccgt ccgaggtcgt cgcagaagcc
    13621 ttcgtgaacg tcagcttgct gaacttcgcc agggggagct gcacacatcg tgaggcgttg
    13681 cagccggtcg gcgcttctgc gatccattcc gcggacgaaa cgtccggctc ggccatgaac
    13741 aacgtcttcg tgaagttcgt cctccgcgtc aggttcctga tctccagagt cacttgcgtg
    13801 ccgtcgacct tgacggaggc cgtgaaggcg ttgcccggga agaccttgaa gcggatgggc
    13861 acactggccg ctgggaccag ttcgtaccac atcgagtact tggccttgcc cgcggtggtg
    13921 caggtcacct gggttccgat ctgctcgagc gcctgcgagg tcggggagaa gccgccgagg
    13981 ccgacccaga atgccgcata cgtcgccttc gacttcgtgc agctcacctt cggctgaacc
    14041 cagctacccg aaacgctcgt aaagctcgtg ggggtcgtcg gagccggagg agccggagga
    14101 gtcggaggaa tcgtcgacgt ggtcgcgtcg agcggcgtga ctgcgtaccc cgcccagttc
    14161 gagctgctcg caatcgcatg tcgcagattc tccaactcga tcgcgttgtt cgtcacggta
    14221 cgaccgctcg ccgctgctgc gctctgcgta gatgtcgccc caaaggacgt gcagatgagc
    14281 acagcgagcg ccgcgagcac gcgaatgaat cgcgctctga cttggttgag atactgagtg
    14341 gtcgtcatag atttctctcc tcagcttccg ggatatgttc cgagcgttac ctgcaaggtc
    14401 gccttgcgac cgtcctggtt cttgaccacg actgagactt cctggcccgg cttcagcccg
    14461 gccagtacag acgacaggac gttgatggtc gacgtcgcgt ggccgtcgac ggagacgatc
    14521 acgtcgccgg cgtctatccc tgccttcgcg gccgggccac ctgcggagac cgagccgaca
    14581 tagagccctt tcccgctggt ctcaccgagc ttgaggccga gataggcgcg gtggtagttg
    14641 acgaccttgc cgttcgtgac gatctggcgg gcgatgtcct tggcaaggtt gcccgggatt
    14701 gcgaacccga tgcccggagc gctgccgccg agctcgggat cggtcgccgc cagggtggga
    14761 atcccgatca cgcgaccctg gatgtcgacc agcgcgccgc cggaattgcc cgggttgatc
    14821 gcggcgctcg tctgaatgac ggacggcaac gtgacgccgt tgcggccttc agacacatcc
    14881 tgccggaagg cactgacgat cccgtccgtc acgctggaac gtagtccgag cgggttgccg
    14941 atggcgatcg cgaggtcgcc cacgttgagc ttcgacgagt cgccgaacgt tgccgccttg
    15001 agatcggcac ctgagatctt gatcacggcg aggtcgtcgg acggaaatgt ccccacgagc
    15061 gtggccgggt attggctgcc gttcgaggta gtcaccgtca gcgatttcgc gccggtgacg
    15121 acgtggttgt tcgtgacgat gtagcccgcc gcgtcgagca cgattcccga gccgagcccg
    15181 acctgatctt gaatttgcac gaccgagggt gataccgact gcacgacctt cacgatcgcg
    15241 ccctgcagcg cgaggcccgg cgtgaccgtt ccactgtcga ccgtgttcgt tgacgaagac
    15301 gacgacgacg agcccatcgc gagcgccgca gccacgacga tgccgccggc gacgaccgcg
    15361 gcgaccagtg gtccgagcag gagcctgtag cgcccccaag caaggatggg attaagcatc
    15421 ggaacctcca acgggtcgat ggcggtggtg agaatcgccg acgagagcgt ccgcgtctgc
    15481 acggccacct tccgtttgct gcacccgcga gccggaaaac gccaataccc gctgccactg
    15541 ggctcagcga agcgctgccg cgatcgaacc gacgtcggtc atgacgtcgt ggcgtcattt
    15601 caacctgtcg ccccgagcgc cggggaggtg acgtcgtcat cggacgatct ccaaggccag
    15661 ctccggcgag cagccgcgag cgacgagatc tgcgatctcg tgccagtcga cgatgcccgc
    15721 gaaggcctcg gcaaacgttc gccgcacacc gagccgccgc aactgctcgg cgcgccactc
    15781 gtgaacgagg agctcctcgt cgtcttgttc ggcggtgggc atcgacatgg gttccatcgc
    15841 tctgtcctcc aggatctggg gtagccgcgg ttgctgtcga agccttgcgc gcgccggggt
    15901 cgttgtcatc ggaggcagca ccgatcgggc cgcatggcta gctgaactct ccagattcct
    15961 gctagctgca atgcggaacg ccgtgtggct tgccacgctg attgggtggc tatcagcgtc
    16021 ctcatcgtcg atgacaacgg gtccttcctc gatgcggcgc gagttctcct ggagcgagag
    16081 ggtctgagcg tcgtcggtgt cgcctcgacc ggggcggagg cgctgcggcg agccgaggag
    16141 ctcagccccg acgtcctcct cgtcgacatc acgctcgggc gggaaagcgg cttcgagttg
    16201 acacgccgtc tggtcgagga cggccgcggc aagcgatcgg cggtaatcct gatctcaacc
    16261 cattcggagg ccgacttcgc ggatctgatc gccgagagcc aggcaatcgg gtttgtgccg
    16321 aagtcggagc tgtcggccga agcgattcgc cggatcctcg accgcggttc ccgctgagcg
    16381 ctaccgtcac tggcatgagc gcgctgaggc gtctggtcgg cgcctcgttg aggcagcggt
    16441 cgcctgcccg tgcgcgtcgc ctcctgcggc tgcctcgccg acaccgcgcg gctgcgagag
    16501 tcctggcggt cgtcggcccg gtggtggtcg ccgttggctt gctgcccttc cgttcgtccc
    16561 tcgggctggc cggcgtcctc ttttgcacgc ttctcgccgt cgtcgcggtc gcgttgatcg
    16621 gtggcgtcgg ggcggcgctc accgccgtcg tggtcggatt tctggcggga gcgctcttgt
    16681 ttgcgcatcc ctatggcagt ctgcgtcccg acagcccggt cgacgttgtc gctctgatcg
    16741 cgttcgtcgt cgttggaggt gtcatcggga tcctcgtcga cgacctcacc cggctcggcg
    16801 aggagcaggc ggccttgcgg cgggtcgcga cgctcgtggc gcgcacagcg ccggctgacg
    16861 aggtgttcgc agcggtcacc gaggaagccg gtcagctgct tcccgtcgat cgaacgagct
    16921 tgagtcgcta cgagtcggac ggcacggtga ccgtcgtcgc cagttggagc agagacggcg
    16981 gccatgtccc cgtcggcacg cggacaatgc tcgggggcaa gaacctcagc accttcgttt
    17041 cggagaccag ccgtccggcc cggatggata gctacgcgga catctccggc gccctcggcg
    17101 cggccatccg cgagacgggt ccacgctcag gggtcgggac accggtcatc gtcgccggtc
    17161 gtctgtgggg tgtcctgatc gccgcctcta cgtcggagcg acggctgcca agcgacctgg
    17221 aggcgcgcct tgccgacttc accgacatcg cggcaacagc cgtcgcgaat gccgagaacc
    17281 gcgctgatct cgcggcctcc cgcgcgcgga tcgtcgcgac tgccgacgag acccggcggc
    17341 ggatcgagcg cgatctgcac gacggagcgc agcagcggct cgtctcactg gcgctcgagt
    17401 tgcgagccgt gcaggcgatg ctcccaccgg agttcggcga gctcgacggc gagttgtccc
    17461 gcgtggccga gggcctcacg agtgtgctgg acgagttgag ggagaccgct cgcgggatcc
    17521 acccggcgat cctggccgag ggcggcctcg gtccggcgct gaagacactg gcccgccggt
    17581 ccgcggtccc ggtcgagctg gacgtccgag tcgacgcgag gctcccggag cggatcgagg
    17641 tggcgacgta ttacatcgtc tcggagacgc tgacgaatgc cgccaagcac gcccacgcct
    17701 cgatcgtccg cgtcggcgtg gaggcggtcg acggtgtagt tcgcgtctcc gtccgggatg
    17761 acggtgcggg gggtgcggat tcggcaaggg gcaccggcct gatcgggctc agagaccgtg
    17821 tggaggcgct gggcggagcg attgcggtcg aaagcccgct cggcgcgggg acagctgtgc
    17881 ggatcgagct tccgaccgct tccagctagc cgtaagcaga acttcccggc aggccggtac
    17941 tgatgtttgc cgctcgccct gacgacacgg cgagaaccgt ccgccatcgt gccctgcatg
    18001 accctttccc atgctcaggc gctgttccga gaggccgtcc acgacctcgc cggggaacca
    18061 accccggcga acgtgcagcg ctatctcgcg gcaagtcaga tcctcgaccg cgccgagccg
    18121 gcggaggccg cgacgaattc gagatcgaac ggtgcggcga cgggcgaaga ccagccggca
    18181 ggtgcgctgg cactggagag gagttgagca tgcgaggcga accggcgatg gcaacgagga
    18241 tcggcttcat cggcctcgga cgcatgggcg gcaacatggc ggcgcgcctt ctcgccgccg
    18301 gctatccgat ctacggcgag gagcaaactc gagcagacgc tcaggggttg atccagaggg
    18361 gtttgcgctg gcgggacacg ccgcgtgggg tcgccgaggc tgccgacgta gtctttacat
    18421 ccctgcccga cgacgaggtg ctcgaggtcg ttgcgtcggg acccgacggc atcttggccg
    18481 gcctagccgc cgggaagatc tgggtcgaca tgagcaccgt cagcccgcgt gcgagtcgcg
    18541 agctcgcggc gagggtgcgg gagctcggag cgtcgatgct cgatgcgccg gtgtcgggaa
    18601 gcgttccgca ggtgcaggcg ggaaccctca cgatcatggt cggcggcgac gagcaggcgt
    18661 acgcgcaggt cgagccgatc ctgcgcgagc tcggcacgcc gacgcacatc ggcgagaacg
    18721 gacaagggct ggtcctgaag ctcgcgatca acatcagtct cgccgtgcaa atgctcgcct
    18781 tcgccgaggg actggtcctg gccgatcgcg ccggaatcga ccgggagctc gccgtcggcg
    18841 tcatgacgca gagcgcgatc ggctccccga tgctgaaggc tcgtgccgag ctcgtccttg
    18901 accttcccga agacgcctgg ttcgacgtca gcctgatgca gaaagacgtg gtgctcgcgc
    18961 tcgacgcagc acgagagctg catatccccc tgccgtcgac ggccgcggcc gacgaggtgc
    19021 tcaccgtcgc gcgcgcactg ggatacgagc atcgcgatct ggccgccatg ttcgaggtgc
    19081 ttgggcagct ggcaggtggc cctgcacttt ctcaggcgtg actgaccaga ttcggcgagc
    19141 aggtccgccg ggcaacgagc gcctgacgac gtcggtcggg ctggtcctgc tcgtcctgct
    19201 catcgcggag acgctgacga ctctgtccgc ccggtcgttg ctgcccgtgc acctctttct
    19261 cgggctcctg ttgctaccgc ccgtcgcgct caagctcgcc agcaccggct ggcgattcct
    19321 gcgctactac gcgggcgaca ggttgtacag acaggagggg ccgccaaggc ttctgctgcg
    19381 gttgctcgct ccggttctgg tcgcttcgac gctcgtgctc ttcggcagcg gaatcgcact
    19441 ggccgtcgtc gggcacggcg gagtctggtt cgcagtgcac atcgtcagct tcggcgtctg
    19501 ggggctgttg atgatcgtgc acgtcctggt ttacctcaca cgtacgctgc gggacggcac
    19561 cgcagactgg caccgccgcg tggagcgggt cgttgtcgga gcgcgccgcc ggcgggcagc
    19621 gctgggtggc gccgtgctgg cgggtgtgat tctcgccgtc gccacctatc cggcccagca
    19681 aggctgggtg agccaccgag gggatcgaca ccgtgacggt ctcgggtccg ctccgacttt
    19741 cgcgctgcga gtgaacgact gaggaacagt cggagagctc caagcgttta tataggtgag
    19801 ggcgtttgtg ccccagtacc ttttgaagga gaactcatga ctagccatcc agacctgcat
    19861 ctcatcgatg tcc
//
LOCUS       DASVEU010000143         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1456733,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000143 DASVEU010000000
VERSION     DASVEU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456733"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1566)
                     /locus_tag="VF225_05720"
     CDS             complement(265..1566)
                     /locus_tag="VF225_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7144790.1"
                     /translation="MNSLQARLRLAIGAAVLGAVSLSLLVGAYLVRRSLEQSAFDGLR
                     RQVALLANEHVHPPAGSFGRFLATQDERLSVLPRAQARLLVPDRPTGRITINGHNYLY
                     ASKSTDSDVVVLLRTASSVRAENQPFWIALAAAGFLGCILAAGVAAVLARSISRPVLR
                     VARASSRLADGEDPEPLPLGGSSELHGLALSFNTMAMQLARARSAERSFLLSVSHELK
                     TPLTAIRGYSEALDEGVLTSERAGKVIRTEAARLERVIMDLINLARLDQRRFDIFLEP
                     VDLAGIARESAMRHAAGARELGVRLDVQQGDEPAPALADRDRLLQALSNLLENALRCT
                     PSGGTVTLAAAAGKLTVKDTGPGIGPDEVPRAFERFFLYRRYNGHRPVGTGLGLAIVR
                     ELALAMGGDVAVASSSSGTEFTLRLPLPERANEHRALHVLT"
     gene            complement(1563..2240)
                     /locus_tag="VF225_05725"
     CDS             complement(1563..2240)
                     /locus_tag="VF225_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144791.1"
                     /translation="MSGLAVVIEDDQAISALVATYLEQAGFEVVCECTGEGGFKAVDR
                     RQPRFVVLDLGLPDVDGFDLCRRLRRSADVPILILTARDEEADRVIGLELGADDYLTK
                     PFSPRELVARVRAVLRRADPGPPESRVIELGELRVDLRTRCATFAGSRLALRTLEFEL
                     LAELARSAGNVVTRDRLLDRVWGLSFAGGTRTVDVHVAQLRKKLGRPDLIHTVRGVGY
                     RLLDSQT"
     gene            complement(2237..2521)
                     /locus_tag="VF225_05730"
     CDS             complement(2237..2521)
                     /locus_tag="VF225_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144792.1"
                     /translation="MRRLWLVLLCAALLPATLARAAGASEDRGIVVRVQPPRFAIREL
                     DGSRVGFRINRATVVTLNGRRVRLRRLQPGDVLAVDHIGRLATAIRAIRP"
     gene            2750..3061
                     /locus_tag="VF225_05735"
     CDS             2750..3061
                     /locus_tag="VF225_05735"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7144793.1"
                     /translation="MVQYPTTVDLAFVALADPTRRAVLERLGSGRATISELAEPFGMS
                     LTGMKKHIRLLEEAQLVTTEKVGRVRRCMLAPYAFEGISTWLQRLDRFAQVVERTKGD
                     R"
     gene            3058..>3374
                     /locus_tag="VF225_05740"
     CDS             3058..>3374
                     /locus_tag="VF225_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7144794.1"
                     /translation="MSTTTKKGKQGSGKSTNKKEGFTAEEKAAVRARARELKDKTDGE
                     TAVRAAIAAMSPKDRAIAKRLHELVKAAAPDLSPKTWYGMPAYAKDGKVVCFFRNAGK
                     FKE"
BASE COUNT          603 a         1101 c         1167 g          503 t
ORIGIN      
        1 cgcccggatg ctgtcgttgc cgccccttgc gtagatgatg tccggcccgg acgtgccgtt
       61 gagactgtcg gggccgtccg tgccgcggat gacatcgagc ctcgccgcgg acgccgcagc
      121 cgcgagcacg agccctgcga ctaccgctag aaggatcagc ttcgctttca aaatgaacct
      181 ccgcatcttg aaggccggtt gacccgtccg ttatcccagc tgcgtatgtg aacggtgtga
      241 tccgcacgta agctcgcgtc acgcctacgt aagcacgtgt aaagcgcggt gctcgttcgc
      301 gcgctcgggc agcgggagtc gcagcgtgaa ctcggttccg ctcgaggagc tggcgacggc
      361 gacgtcaccg cccatcgcga gggcgagctc gcggacgatc gcgaggccga gaccagtgcc
      421 caccggacgg tggccgttgt aacgccggta caggaagaag cgctcgaatg cgcggggcac
      481 ctcgtcaggg ccgatgcctg gacccgtgtc cttcaccgtg agcttgcctg cagccgcggc
      541 cagcgtgacc gtgccaccgg atggcgtaca gcgcaaggca ttctcgagga ggttcgacag
      601 cgcctgcaac aggcggtcgc gatcggccag cgccggagcg ggctcgtcgc cctgttgaac
      661 gtccaagcgg acgccgagct cgcgcgcgcc tgcagcgtgg cgcatcgcgc tctcgcgcgc
      721 aatccccgcc agatcgacgg gctcgagaaa aatgtcgaag cgtcgctggt cgagtcgcgc
      781 gaggttgatg agatccatga tcacgcgttc gagtcgtgca gcttcggtcc ggatcacctt
      841 tccggcgcgc tcggacgtca agacgccttc gtcgagcgct tctgagtagc cgcggatcgc
      901 cgtcaaggga gtcttcagct cgtggctcac cgacagtagg aacgaacgct ctgcgcttcg
      961 cgcacgcgcc agctgcatcg ccatcgtgtt gaatgacagc gcaaggccgt ggagctcact
     1021 cgaaccaccc agcgggagtg gctctgggtc ctcgccgtcc gcaaggcggc tgctcgcgcg
     1081 cgcgacgcgt agaactgggc gcgagatgct gcgggcgagg accgccgcaa cacccgcggc
     1141 gagaatgcag ccgaggaaac cggcagctgc cagcgcgatc cagaagggtt ggttctccgc
     1201 gcgcaccgag ctggcagtgc gcaggagcac gacgacatcg ctgtcggtgc tcttgcttgc
     1261 gtagaggtag ttgtgcccgt tgatggtgat gcgccccgtc ggacggtcgg ggacgaggag
     1321 gcgtgcttgc gcccggggca ggacggagag gcgctcgtcc tgggtggcga ggaatcgccc
     1381 gaaggagcct gccggaggat gcacgtgctc gttcgccagc agtgcgacct gtcgccggag
     1441 accgtcgaag gcgctctgct cgagcgaccg gcggacgagg tacgcgccga cgagcaacga
     1501 gagcgagacg gcgccgagca cagcagcgcc gatcgcgagt cggagccgtg cctgcagcga
     1561 attcacgtct ggctgtcgag gaggcggtag ccgacgccgc ggaccgtgtg gatgaggtcc
     1621 ggacgcccga gcttcttgcg cagctgggcg acgtggacgt cgacggtgcg cgttccacct
     1681 gcgaacgaga gtccccagac gcgatcgagg agccggtcgc gcgtcacgac gttgccggcg
     1741 ctgcgcgcga gctccgcgag cagctcgaac tcgagtgtcc gcaatgcgag ccgcgagcca
     1801 gcgaatgtcg cgcaacgcgt tcggaggtca actctcagct cgcccaactc gatgacacgc
     1861 gactccggcg gcccggggtc ggcccttcgc aggaccgcgc ggacccgcgc cacgagctcg
     1921 cgcggcgaga acggcttggt gaggtagtcg tccgcgccga gctcgaggcc gatgacccgg
     1981 tccgcttcct cgtcgcgcgc ggtgaggatc aggatcggga cgtccgcgct gcggcgcaat
     2041 cgtcgacaga ggtcgaaccc gtcgacgtca ggaagcccga ggtcgagcac gacgaaacgc
     2101 ggttgccgtc gatcgaccgc tttgaatccg ccctcgccgg tgcattcaca caccacctcg
     2161 aagcccgcct gctccaggta cgtggcgacg agcgcgctga tcgcttggtc gtcttcgatg
     2221 acgaccgcga ggccgctcac ggccgaatcg cccggattgc ggtcgcgagt cgcccgatgt
     2281 ggtcgacggc cagcacgtcg ccgggttgaa ggcggcgaag gcgcacgcgc cgcccgttca
     2341 gcgtgacgac ggtcgcccgg ttgatcctga atcccacgcg actcccgtcg agctcgcgga
     2401 tggcgaagcg cggcggttgg acgcgcacga cgatgccgcg gtcttcgctc gcgccggcag
     2461 ctcgagcgag ggtcgccggc aggagggcgg cgcagagcag caccagccag agccgtcgca
     2521 tggccaggat cctggtcgac ccggatcagc ctggagtgtg gtctatgtaa agcggacgta
     2581 aaacgcccgg ttcgtcgata cgaaaggtga tgctggtggc gtggccccgg gcggactgtg
     2641 tcctgcgcca cgccatctgc ccggatcgat cgccgcatga gccatcagcc cgcacttgcc
     2701 gacgcggcgg tattgcgtcg cagcgccgaa atccactaca gtgaacctaa tggttcagta
     2761 tccaacgacc gtcgacctcg ctttcgtagc cctcgccgac ccgacccgcc gcgccgtcct
     2821 cgaacggctc ggttccggta gagcgacgat cagcgagctc gccgagcctt ttggcatgtc
     2881 gctgaccggg atgaagaagc acatccggct gctcgaggag gcgcaactcg tgacgacgga
     2941 gaaggtcggc cgtgtccgca ggtgcatgct cgccccctac gctttcgagg gcatcagcac
     3001 gtggctgcag cggctcgacc gcttcgcgca ggtcgtcgaa cgcacgaaag gagaccgatg
     3061 agcaccacta cgaagaaagg caagcaaggg tccggcaaga gcaccaacaa gaaagaagga
     3121 ttcacagccg aagaaaaagc cgcggtgaga gcgcgagccc gagagctgaa ggacaagacg
     3181 gacggagaaa ccgccgtgcg cgccgcgatc gccgcgatgt cgccgaaaga tcgcgccatc
     3241 gccaagcggc tccatgagct cgtcaaagcg gccgccccag acctctcgcc gaagacctgg
     3301 tacgggatgc cggcgtatgc caaagacggc aaggtcgtct gcttcttccg caacgcgggg
     3361 aagttcaagg agag
//
LOCUS       DASVEU010000144         6630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_488604,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000144 DASVEU010000000
VERSION     DASVEU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488604"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VF225_05745"
     CDS             complement(<1..989)
                     /locus_tag="VF225_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7144795.1"
                     /translation="METVSFARGIPAPECLPVEELADCARAALERDGATVLSYGSSAG
                     YGPLRDWIATRHGIDPTRVLVTNGSLQGMVFFAERFAGERVLVEAPTYDRPLKILAAR
                     GVETTPVAMDDEGLDLDALTHALDSGRKPAFLYTIPTFQNPSGRTLSEERRHRLAELA
                     RAHQLLVLEDDPYGLVRFDGEAPPTLFELEGGKHVAYSSSFSKTIAPGLRVGYFVLPE
                     VLEQELEALATSTYITPVLLGQATVFEFLRRGNFEPNLERVATLLGARRDAMLEALER
                     ELPDARWSRPQGGYFIWLELPEGTDAGALLDRAAAAGVTYVPGADFGGGPNTAR"
     gene            complement(1000..1878)
                     /gene="sucD"
                     /locus_tag="VF225_05750"
     CDS             complement(1000..1878)
                     /gene="sucD"
                     /locus_tag="VF225_05750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7144796.1"
                     /translation="MAIIVDHGTRLVVQGLTGSEGRFHGLRNRRYGTQVVAGVTPGKA
                     GQDVEGIPVYNTVADAVHETEANTTLIFVPARFATDAIYEAVDAGIGTVICITEHIPA
                     HDMLRVYTYIRDRGVTMIGPNCPGVLSPGKANVGIIPAEIFSEGAIGLVSRSGTLTYQ
                     IGHELTQLGLGNSTIVGIGGDPVVGSSFIDVLQRFETDHETELIVLVGEIGGDEEEKA
                     ARFVQDHITKPVIAYIAGFSAPPGKTMGHAGAIISGSSGTAQAKKEALEAAEIQVGTT
                     PTAVAQLVAERFGARA"
     gene            complement(1878..2528)
                     /locus_tag="VF225_05755"
     CDS             complement(1878..2528)
                     /locus_tag="VF225_05755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144797.1"
                     /translation="MTDVWSQRADAFRESPTHREGPDLDLLVEWCDPGHDVKVLDVAT
                     GGGHVARRLREAGCTVVTVDPAPGMEPDVVSRAEELPFEDGSFDVVTCRIAAHHFQDI
                     RKAVAEMARVTQAVVVIEDNVFVDERVEEAERLRDPTHVRCYSEDEWKEMLTEAGLEV
                     EQLERFERHPVVEGWLARVDTPPEDAARVRELLADHVQDGALSLRSIVVKARISQT"
     gene            complement(2525..3667)
                     /gene="sucC"
                     /locus_tag="VF225_05760"
     CDS             complement(2525..3667)
                     /gene="sucC"
                     /locus_tag="VF225_05760"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7144798.1"
                     /translation="MDLYEYQGKQLFARYGIPVSEGRLATTPEEARAAAEEIGGTVVV
                     KAQVLTGGRGKAGGVKLADDPADSEAKARDILGLDINGHVVRKVWIEQASEIAKEYYL
                     SITFDRGAKQPLYMLTTEGGVEIEQVAEQNPEALARLHVDPLEGFQPYQARRLIYGAG
                     IDDPSEQKQILGIIEKLYDCFVESDAMLTEINPLIVTPGGDVRALDAKFTVDDSALFR
                     HPDIAEMRDVEAADPQERLAREKGVTYVKLDGDVGILGNGAGLSMSTVDVVVVAGGRP
                     ANFCDLGGGGDAQGVVDALEVITSDKQVRSIFFNIFGGITRGDEVARGILTALDQMRI
                     DLPIVVRLDGTNAEEGRRLLAEAAPPNLHVEPTMLKAAQRAVELAA"
     gene            complement(3864..5630)
                     /locus_tag="VF225_05765"
     CDS             complement(3864..5630)
                     /locus_tag="VF225_05765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7144799.1"
                     /translation="MAVFSKVLVANRGEIAIRVFRTLRELGIGTVAVYSDVDRNSAHV
                     SYADEAHLIGAGPPAESYLNQERILDAAQRAGAEAIHPGYGFLAENADFARDCGERGL
                     VWIGPPPDAIEAMGSKIEARVRMRAAGVPIVPGVTQPVESAEEVRTLGDELGWPVAIK
                     ASAGGGGKGLKVVRDPGEVGRAFESARREGEAYFSDPAVYVERYLEDPRHVEVQVLAD
                     AHGNVIHLGERDCTIQRRHQKLVEETPSPAVDSGLRERIGAIAVDAARAVGYQSAGTI
                     EGLLSRDGEYFFLEMNTRIQVEHTVTEMVTGLDLVREQVLIASGRPLSVRQQDVHLHG
                     HALECRINAEDVSNGFLPAPGTITRYHEPSGPGVRVDSGVIAGSEISPLYDPMIAKLV
                     VHDVDRERARRRMLRALDEFVIDGVRTLIGFHKALLTHPCFVEGETCHGLVESELLAA
                     RAGEFENGRRVGPPELGTAALREAVRSVEVDGRRFEVRVLRPEPAFAELGRRRRERAR
                     GSGAGGAGRDAVLSPMQGTVLAVEVAEGDEVQAGQVICIVEAMKMENEVHAHRAGTVS
                     ELSVVPGQPVTTGQVICVVTAE"
     gene            5741..6283
                     /gene="pgsA"
                     /locus_tag="VF225_05770"
     CDS             5741..6283
                     /gene="pgsA"
                     /locus_tag="VF225_05770"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7144800.1"
                     /translation="MSLPDGLTVARAASVPFVILLYALDFHGHNYWATAVFAAAMATD
                     WFDGRIARSRGHSSPLGSLLDPAADKILVLAVLIMLVDQRVAPGWMVAAIVVREFLVT
                     ALRLAALERGVVLQARDLGKLKTWSQAVAAAVGGFAAAGAWRSDIAWWTLLVAVVLTW
                     ISGLDYARVAPRLWRGGATA"
     gene            complement(6435..>6630)
                     /locus_tag="VF225_05775"
     CDS             complement(6435..>6630)
                     /locus_tag="VF225_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144801.1"
                     /translation="EHDERADVRKAAAAHTCPIGGQPYLFNVGLVWTLRKVCGAEKLV
                     KTVAGAKPARPRCGAAESAP"
BASE COUNT          982 a         2364 c         2147 g         1137 t
ORIGIN      
        1 cgcgcagtgt tcggcccacc tccgaaatcc gcaccgggca cgtacgtcac gccggccgcg
       61 gctgcacggt cgagcaacgc accggcatcg gtgccttcgg gtagttcgag ccagatgaag
      121 tagccgccct gaggccggct ccaacgtgca tccggaagct cacgttcgag tgcttcgagc
      181 atcgcgtcac gacgggcgcc gaggagtgtc gcgactcgtt cgaggttcgg ctcgaagttg
      241 ccgcggcgca gaaactcgaa caccgtcgcc tgccccagta ggaccggcgt gatgtaggtg
      301 gaggtcgcca gcgcctcgag ctcttgctcc aaaacctcgg gcaggacgaa gtagccgacg
      361 cgcaaccccg gcgcgatcgt cttcgagaag gaggacgagt aggcgacatg cttgccgcct
      421 tccagctcga acagcgtcgg cggcgcctcg ccgtcgaagc gaacgagccc gtacggatcg
      481 tcctcgagca ccagcagctg gtgcgcgcgt gcgagctcgg caagtcgatg acgacgttcc
      541 tcgctgagcg ttctcccgct cgggttctgg aaagtgggaa tcgtgtagag gaacgccggc
      601 ttcctgccgc tgtcgagcgc atgtgtgagt gcgtccaggt cgaggccctc gtcgtccatc
      661 gccaccggcg tcgtctcgac gccccgcgca gcgaggatct tcagcgggcg atcgtaggtc
      721 ggcgcctcca cgaggacccg ctccccggcg aaccgttcgg cgaagaagac cattccctgc
      781 agcgagccgt tggtgaccag gacccgcgtg ggatcgatac cgtgacgcgt cgcgatccag
      841 tcgcggagcg gcccgtaccc ggccgacgaa ccgtacgaga gcacggtggc tccgtcgcgc
      901 tcgagcgcgg cccgcgcgca gtctgcgagt tcctcgaccg gaagacattc gggggcgggg
      961 atgccccgcg cgaaggagac cgtctccatc agcgagagtt taggcgcgcg ccccgaatcg
     1021 ctcggccacg agttgcgcga ccgcagtcgg cgtcgtcccg acctgaatct ccgctgcttc
     1081 cagtgcttcc ttctttgctt gggccgttcc cgacgacccc gagatgatcg cacccgcgtg
     1141 acccatcgtc ttgccggggg gtgccgagaa cccggcgatg taggcgatca ccggcttcgt
     1201 gatgtgatcc tgcacgaagc gggcggcctt ctcctcctcg tcgccgccga tctcgcccac
     1261 gagcacgatc agctccgtct cgtggtccgt ctcgaaccgc tggaggacgt cgatgaagga
     1321 cgagccgacg accggatcgc cgccgatgcc gacgatcgtc gagttcccga gccccagctg
     1381 agtgagctcg tggccgatct ggtacgtgag cgtgccagag cgtgagacga ggccgatcgc
     1441 accctcggag aagatctcag cggggatgat cccgacgttc gccttgccgg gggacagcac
     1501 gccgggacag ttcggcccga tcatcgtcac gcccctgtcg cggatgtacg tgtagacccg
     1561 cagcatgtcg tgagccggaa tgtgctcggt gatgcagatc acggtgccga tgccggcgtc
     1621 cacagcttcg tagatcgcgt cggtcgcgaa gcgcgccggc acgaagatga gcgtcgtgtt
     1681 cgcttctgtc tcgtgcaccg cgtcggcgac cgtgttgtag acgggaatgc cctcgacgtc
     1741 ttgccctgcc ttgcccgggg tgacgccggc gaccacctgc gttccgtacc tgcggttgcg
     1801 cagaccgtgg aagcggcctt cgctgcccgt caggccctgc acgacgaggc gcgtgccgtg
     1861 gtcgacgatg atcgccatta cgtctggctt atccgggcct tgacgacgat cgacctcagg
     1921 ctgagcgcac cgtcttgcac gtgatccgcg aggagctcgc ggacgcgcgc ggcgtcctcg
     1981 ggcggcgtgt cgactcgcgc gagccagccc tcgaccacgg ggtgacgttc gaagcgttcg
     2041 agctgctcga cctcgaggcc ggcctccgtg agcatctcct tccactcgtc ctccgagtaa
     2101 cagcgcacgt gcgtcgggtc gcgcaggcgc tcggcttcct cgactctctc gtcgacgaag
     2161 acgttgtcct cgatcacgac gacggcctgc gtgacgcgcg ccatttcggc gaccgccttg
     2221 cgaatgtcct ggaagtgatg ggcggcgatc ctgcaggtga cgacatcgaa gctcccgtcc
     2281 tcgaacggca gctcttcggc gcgagagacg acgtccggct ccatgcccgg cgccgggtcg
     2341 acggtgacga ccgtgcagcc tgcctcgcgc agtcgcctcg cgacgtggcc tcccccggtc
     2401 gcgacgtcga gcaccttcac gtcgtggccg gggtcacacc actcgacgag cagatcgaga
     2461 tccggtcctt cacgatgggt cgggctctca cggaaagcgt ccgcacgctg cgaccagacg
     2521 tcggtcacgc cgccaactcg accgcgcgct gcgcagcttt cagcatcgtg ggctcgacgt
     2581 ggaggttggg aggcgctgcc tcggcgagca gcctgcgccc ttcctcggcg ttcgtcccgt
     2641 cgagcctgac gacgatcggg aggtcgattc tcatctggtc gagcgcggtc aggattccgc
     2701 gcgccacctc gtcgccgcgg gtgattcccc cgaagatgtt gaagaagatc gagcggacct
     2761 gcttgtcgct cgtgatcacc tcgagcgcgt cgaccacccc ctgcgcatcg ccaccgccgc
     2821 ccagatcgca gaagttcgcc ggcctgccgc ccgcgacgac gaccacatcc acggtcgaca
     2881 tcgacaggcc cgccccgttc ccgaggattc cgacgtcgcc gtcgagcttg acgtaggtga
     2941 cgcctttctc ccgcgcgagc cgctcctgcg ggtcagccgc ctcgacgtcg cgcatctcgg
     3001 cgatgtcggg gtggcggaag agcgcgctgt cgtcgacggt gaactttgca tcgagcgcgc
     3061 gcacgtctcc gccgggagtg acgatcagcg ggttgatctc agtgagcatc gcgtccgact
     3121 cgacgaagca gtcgtacagc ttctcgatga tgccgaggat ctgcttctgc tcgctcggat
     3181 catcgatgcc tgcgccgtag atcagtcggc gggcctggta cggctgaaag ccttcgagcg
     3241 gatcgacgtg caggcgggcg agcgcctctg gattctgctc ggcgacctgc tcgatctcga
     3301 ccccgccctc ggttgtgagc atgtagagcg gttgcttcgc gccgcggtcg aaggtgatcg
     3361 agaggtagta ctccttcgcg atctccgagg cctgctcgat ccagaccttg cgtaccacgt
     3421 ggccgttgat gtcgagcccg aggatgtcgc gcgccttcgc ttccgagtcg gcgggatcgt
     3481 cggcgagctt cacgcctccg gccttgccgc gcccgccggt cagcacctgg gccttgacga
     3541 cgaccgtgcc cccgatctcc tcagccgccg cgcgcgcctc ctcgggcgtc gtcgcgaggc
     3601 ggccttcgga cacggggatg ccgtagcgcg cgaaaagctg cttgccctgg tactcgtaga
     3661 gatccaccgg cgcggcactc tacccgcgcc ccaccgcgcg tcgccgcgcc tcgagatcgc
     3721 cgcttgtctc gagatcgccg catgtctaga ggtacacgcg tgcgcgcggc cccaccctgg
     3781 gtgggggtca tcacccacca ccctctgcga gcgacgatac ccgcgagagg cctacgtgtc
     3841 tgcgccggtt tagcgccgtc atctcactcg gccgtcacga cgcagatcac ctggcccgtc
     3901 gtgaccggct gcccggggac gacggacagc tcgctgacgg taccggcgcg gtgcgcgtgc
     3961 acctcgtttt ccatcttcat ggcctcgacg atgcagatga cctggcccgc ctgaacctca
     4021 tcgccttctg ccacctcgac ggcaagaacc gttccctgca tcgggctcag caccgcgtcg
     4081 cggcctgcgc ccccggcccc gctcccacgc gcacgctcac gacggcgcct ccccagctcg
     4141 gcgaaggccg gctccggcct gaggacacgc acctcgaacc gacgtccatc gacctcgacc
     4201 gagcggaccg cctcacgtag ggcagccgtg ccgagttccg gggggccaac ccgacggccg
     4261 ttctcgaact cccccgcgcg cgccgccagc agctctgact cgaccaggcc gtgacacgtc
     4321 tcgccctcga cgaagcaggg gtgcgtcagc agagccttgt ggaagccgat caacgtcctc
     4381 acgccgtcga tcacgaactc gtcgagggcc cgcagcatcc gccgccgcgc acgctcgcgg
     4441 tcgacgtcat ggacgacgag cttcgcgatc atcgggtcgt agaggggaga gatctcgctt
     4501 ccggcgatga caccggagtc gacgcgcaca cccggcccgc tcggctcgtg gtagcgcgtg
     4561 atcgtcccgg gtgcgggaag gaatccgttg gaaacgtcct ccgcgttgat ccggcactcc
     4621 agcgcgtggc cgtgcagatg cacgtcttgc tgtcgcacag agagcggtcg ccccgacgcg
     4681 atcaacacct gctcgcgcac aagatcgaga ccggtcacca tctccgtgac cgtgtgctcg
     4741 acctggatgc gcgtgttcat ctccaggaag aagtactcgc cgtcgcgcga gaggaggccc
     4801 tcgatcgtgc cggcgctctg gtagccgacc gcacgcgccg catcgacggc gatcgcaccg
     4861 atccgctccc gcaacccgct gtcgacagcg ggcgaaggcg tctcctcgac gagcttctga
     4921 tggcggcgct gaatcgtgca gtcgcgctcg cccaggtgaa tgacgttgcc gtgcgcatcc
     4981 gcgaggacct gcacctcgac gtggcgcgga tcctcgagat agcgctctac gtacacggct
     5041 ggatccgaga agtaggcctc gccctcgcgt cgggccgact cgaaggcacg ccccacctcc
     5101 cctggatcgc ggacgacctt caaccctttg ccaccaccgc ccgcggacgc cttgatcgcg
     5161 accggccagc cgagctcgtc gccgagcgtg cgcacctcct cggccgattc gacgggctgg
     5221 gtgacgcccg ggacgatcgg gacgcctgcc gcccgcatcc gcacacgcgc ttcgatcttg
     5281 gatcccatcg cctcgatcgc gtcaggcggc gggccgatcc acacgagacc ccgctcgccg
     5341 cagtcgcggg cgaaatctgc gttctccgcg aggaagccgt agcccggatg gatcgcttcc
     5401 gccccggccc gctgcgctgc atcgaggatc cgttcctggt tcagatagct ctcggcgggc
     5461 ggcccggcgc cgatcagatg cgcctcatcc gcgtagctca cgtgtgccga attccggtcg
     5521 acgtccgaat agacggcgac cgtgccgatg ccgagctcgc gcagcgtccg gaagacccgg
     5581 atcgcgatct cgccccgatt ggcgacgagc actttgctga aaaccgccac tgcccctcta
     5641 ttcttgcgat cgtggtcgct cgcctctggc tcgctcccct ggggaaaacc atgttccccc
     5701 cacgtgccgc cccttccact catcggccgg aaagcggcct atgagtcttc cagacggtct
     5761 gacggttgcg cgcgccgcct ccgtcccttt cgtcatcttg ctctacgcgt tggacttcca
     5821 cggtcacaac tactgggcga cggcggtctt cgcggccgcg atggccacgg attggttcga
     5881 cgggcgcatc gcacgcagcc gcggccactc ctcgccgctc ggctcgctgc tcgatcccgc
     5941 ggcggacaag atcctcgtcc tcgcggtctt gatcatgctc gtcgaccagc gggtggcgcc
     6001 cggctggatg gtcgctgcga tcgtcgtccg ggagttcctc gtcaccgcgc tgcgcctggc
     6061 cgcactcgaa cgaggcgtgg tgctgcaggc acgcgatctg ggcaagctga agacgtggtc
     6121 gcaggcggtg gccgcagccg tcggcggctt cgcggccgcc ggtgcctggc gaagcgacat
     6181 tgcctggtgg acgctgctcg tggcagtcgt gctgacgtgg atctccgggc tggactacgc
     6241 ccgggtcgct ccgcgcctct ggcgcggcgg cgcgacggcc tgatcgttct cctcctcggg
     6301 cccggggcct agcccggcgc ggcgccaggg cgagggcgcc gcggcggttc gaccgtcctc
     6361 ttcaagcgct tcgaggatcg ctctgcgttc ttcctgggtc ggttccgggg tgatctggac
     6421 gtccatattt gcgactaagg cgccgactcg gcggcgccgc agcgaggtcg cgcgggcttc
     6481 gccccggcga ccgttttgac gagcttctcg gccccacaaa ccttcctcag cgtccacacc
     6541 agcccgacat taaagagata cggctggccg ccgataggac aggtatgcgc cgccgccgcc
     6601 ttgcgtacgt ctgcgcgctc gtcatgctct
//
LOCUS       DASVEU010000145         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1263666,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000145 DASVEU010000000
VERSION     DASVEU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263666"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF225_05780"
     CDS             <1..534
                     /locus_tag="VF225_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEX7144802.1"
                     /translation="RRDACCAQRGWPVAAVLTEAFPGWVWQCSHVGGDRFAANVVILP
                     QGLYYGRLTRERAAGVVERHLDGRVTVDLLRGRSCFVPAVQAAQHFAREALGIDALDA
                     LGPLEVDRRGDGAVAVRLAGGDVDVRVVLAEGTSAPIERLTCGATQATSVRTWAKLEL
                     TTATRRPAADGSEVSRS"
     gene            709..>2012
                     /locus_tag="VF225_05785"
     CDS             709..>2012
                     /locus_tag="VF225_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7144803.1"
                     /translation="MKHRSMRFVGSVAGALALVASLAAAAVGTAGNETAAAPAPVKPP
                     NVPNAKALKAKYGGTSLTFLGDGPVGKSHKRDQLLVAKFSADTGIKVKLVPHPADSSA
                     SYSQLARTFSAKSSAFDVVMLDVVWPGAFAQYLVDLKSALARDARAHSAGIIRNNTIN
                     GKLVAMPWFGDFGILYYRTDLLKKYGYSSPPKTWAQLGTMAQKIQEGEQASNPNFYGF
                     VYQGNAYEGLTCDGLEWLASSGGGNFIENGKVSINNPRAVAILNLQRSWVGKITPRGV
                     TTYQEGEADSAFGAGNAAFMRNWPYAYSKSQTGPVKGKFDVTVLPRSGNNASVGTVGG
                     WQLGVSAFSKNKPAAIELVRYLTSAPVEKFNAIYNSNVPTIASVARDPAVRKVNPWLK
                     PEIANVARVTRPTVLGTKYAQGSQIIYQGFNQILNGQDAKNVL"
BASE COUNT          335 a          698 c          683 g          296 t
ORIGIN      
        1 aggcgcgacg cctgctgtgc ccagcgcggc tggccggtgg ccgcggtcct cacggaagca
       61 ttcccgggat gggtctggca atgctcccac gtgggtggcg accggttcgc ggcgaacgtc
      121 gtgatcctcc cgcaggggct ctactacggc aggctgactc gcgagcgcgc ggccggggtc
      181 gtcgagcggc acctcgacgg ccgggtcacg gtcgacctgc tccggggccg ctcgtgcttc
      241 gtcccggcgg tgcaggcagc ccagcacttc gcccgcgaag cgctcggcat cgacgcgctc
      301 gacgcgctcg gcccgctcga ggtcgaccgg cggggcgacg gagccgtcgc cgtgcgactc
      361 gccggcggcg acgtggacgt gcgcgtcgtc ctcgccgagg gcacctccgc gccgatcgag
      421 cggctcacct gcggcgcgac ccaggcgacg agcgtccgca cctgggcgaa gctcgagctg
      481 acgaccgcga cgcgacggcc ggccgcagat ggctcagaag tgtctcgttc ttgacaccgg
      541 cttgcgagaa tctctaccgt ggctgtgctg catgacgtct gacactcgtc acgtcgcccg
      601 acgaccgcta gcgggctccg cgcgagcgtc cctgggtacc gaccttctcg cgccgcgccg
      661 acccccagcg gctcactctg cgacgtccgt ctaggaaagg tgggagaagt gaagcatcgc
      721 tcgatgcgct tcgtcggcag cgtggccggg gcactggccc tcgtcgcgtc gctggcagcc
      781 gccgcggtag gcaccgcggg caacgagacg gcggccgcgc cggcgcctgt caagcccccg
      841 aacgtcccca acgcaaaggc cctgaaggcc aagtacggcg gaacgtctct gacgttcctc
      901 ggcgacggtc ccgtgggcaa gagccacaag cgcgatcagc tgctcgtggc caagttctcg
      961 gcggacacgg gcatcaaggt caagctcgtg ccgcatccgg cggactcgtc ggcctcgtac
     1021 tcgcagctgg cacggacgtt ctccgccaag tcgtcggcgt tcgacgtcgt catgctcgac
     1081 gtcgtctggc ccggcgcgtt cgcgcagtat ctggtcgacc tgaagtcggc cctcgcgcgg
     1141 gacgcgaggg cgcactcggc gggaatcatc aggaacaaca cgatcaacgg gaagctggtc
     1201 gcgatgccct ggttcggcga cttcggcatc ctgtactacc gcaccgacct gttgaagaag
     1261 tacggctact cgtcgccgcc gaagacatgg gcccagctgg gcaccatggc gcagaagatc
     1321 caggagggtg agcaggcgag caacccgaac ttctacgggt tcgtctacca ggggaacgcc
     1381 tacgagggcc tgacctgcga cgggctcgag tggcttgcct ccagtggagg cgggaacttc
     1441 atcgaaaacg gcaaggtctc gatcaacaac ccccgagcgg tcgcgatcct caacctccag
     1501 cgcagctggg tcgggaagat cacgccccgc ggcgtgacga cgtatcagga gggtgaggcg
     1561 gactccgcct tcggggcggg caacgccgcc ttcatgcgga actggcccta cgcgtactcg
     1621 aagagccaga ccggtcccgt caagggcaag ttcgacgtga ccgtcctgcc gcggtcgggg
     1681 aacaacgcgt ccgtgggcac ggtgggcggc tggcagctcg gcgtctcggc gttctcgaag
     1741 aacaagcccg cggcgatcga gctcgtccgg tacttgacga gcgcacccgt ggagaagttc
     1801 aacgccatct acaacagcaa cgtcccgacg atcgcctccg tcgcccgcga cccggccgtg
     1861 cgcaaggtga acccctggct caagccggag atcgcgaacg tcgcgcgcgt gacacgcccg
     1921 accgtcctgg gcacgaagta cgcgcagggc tcgcagatca tctaccaggg cttcaaccag
     1981 atcctcaacg gccaggatgc caagaacgtc ct
//
LOCUS       DASVEU010000146         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1070041,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000146 DASVEU010000000
VERSION     DASVEU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070041"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF225_05790"
     CDS             complement(<1..373)
                     /locus_tag="VF225_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX7144804.1"
                     /translation="MSEERRLTIDGVEVHDGGACYVVAEIGNNHQGSLEQAKQLVDAA
                     KECGVDAVKLQKRANRALYTREFFDQPYDNEFSFGRTYGEHREALELSPSDLAELQAH
                     AREVGITFFATAFDFESADLLA"
     gene            complement(370..1350)
                     /locus_tag="VF225_05795"
     CDS             complement(370..1350)
                     /locus_tag="VF225_05795"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7144805.1"
                     /translation="MKRALITGIGGQDGSYLAELLLERGYEVAGMVRPGAVEYPNLEA
                     VRERIELLEADLLDQRSLATALEAARPGEVYNLAAPSFVPASWERPVETAEFAAVGAT
                     SLLEAIRTVDPAIRVYQASSSEIFGEPRQSPQTEETPLEPVTPYGVAKAYAHFIVSSY
                     RRRYGLFACSGILYNHESPRRPLEFLPRKVAHGAAAISLGLQEELVLGDLDARRDWGY
                     AGDYVRAMWLMLQEDEPGDYIVASGTSHSVRELVQCAFAHVGLDWEEHVRVDPALQRG
                     AAELHRLVGDASRAHGRLGWRRELDFEQLVHLLVDADLERLRMPATSMER"
     gene            complement(1347..2360)
                     /locus_tag="VF225_05800"
     CDS             complement(1347..2360)
                     /locus_tag="VF225_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7144806.1"
                     /translation="MKVAVLTTSYPSDRDHGAGRFVADAVERVRARGVAVTVIGPEQF
                     GHFGIAYGHGIVGNLRRRPWRAVLVPALLTNFLRAARRADADLLHAHWLAAGWVAART
                     GKPYVVQVWGTDVELARRAPWLARGVLGRARLVIAASRALADAARSLGAREVRVIPSG
                     VDLPPEVGEEAEPAEVLYAGRLSPEKGVLELLDAAQGLNLVVAGDGPLRDRFPLARGF
                     VQHEELQHLYARAAVVVCPSQREGFGVACLEAMAHGRPVVATHVGGLLDLVVDGETGI
                     VVPPRDPVALRSALDRLLADKELRRRLGLAGRERARTLFSWGDVTDATLAAYAEALGT
                     MER"
     gene            complement(2371..2727)
                     /locus_tag="VF225_05805"
     CDS             complement(2371..2727)
                     /locus_tag="VF225_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144807.1"
                     /translation="MRSRILPWAILLIAAVAYPLGALAHGRPSFPSRADCIRPAKHDG
                     NLEAVFGRFATSARADAVLRRALEVGFKGTQIESDGCGLLKVTLHGIPTLQVGRDFIA
                     EARTAGFNPRLEQSKP"
BASE COUNT          487 a          987 c          959 g          419 t
ORIGIN      
        1 ccgcgaggag atcggcactc tcgaagtcga acgcggtcgc aaagaaggtg atgccgacct
       61 cgcgcgcatg ggcctgcagc tcggcaaggt cgctcggcga cagctcgagt gcctcgcgat
      121 gctcgccgta agttcgaccg aagctgaact cgttgtcgta cggctgatcg aagaactcgc
      181 gcgtgtagag cgcgcgattc gcgcgcttct gcagtttgac ggcgtcgacg ccgcactcct
      241 tggcagcgtc gaccagctgc ttcgcctgct cgagactgcc ctgatggttg ttgccgatct
      301 ccgcgacgac gtagcacgca ccgccgtcgt ggacttcgac cccgtcgatc gtcagccggc
      361 gctcctcgct catcgttcca tgctagttgc gggcatgcgc aggcgttcta ggtcggcgtc
      421 gacaagcagg tgcacaagct gctcgaaatc gagctcccgc cgccagccga gcctgccgtg
      481 cgcacgcgat gcatcgccga ccagccggtg cagctctgcc gccccgcgct gcagcgcagg
      541 atcgacacgg acgtgctctt cccagtcgag cccgacgtgc gcgaaggcgc actgcacgag
      601 ctcccggaca gaatggctcg tgccggacgc gacgatgtaa tccccgggct cgtcctcctg
      661 caacatcagc cacatcgcac gcacgtagtc gcccgcatag ccccagtcgc ggcgagcgtc
      721 gagatccccg aggacgagct cctcctgcag accgagagag atggccgcgg cgccatgcgc
      781 gaccttgcgc ggcaggaact ccagcggccg ccggggcgac tcgtggttgt agaggatccc
      841 cgagcatgcg aacaggccgt accggcggcg atacgagctg acgatgaagt gcgcgtatgc
      901 ctttgcgacg ccgtagggcg tcaccggctc gagaggcgtt tcctccgtct gggggctctg
      961 gcgtggctcg ccgaagatct cgctcgacga cgcctgatag acgcgaatcg cgggatcgac
     1021 tgtcctgatc gcctcgagca gcgacgttgc gccgaccgcg gcgaactccg cggtctcgac
     1081 cggccgttcc caggacgcag ggacgaatga gggagccgcg aggttgtaga cctcgccagg
     1141 gcgtgcagcc tccaatgccg tggccaggga gcgctgatcg aggaggtcag cctcgagcaa
     1201 ctcgatccgt tcgcggaccg cttcgagatt cggatattcg accgcgccgg gccgcaccat
     1261 ccccgccacc tcgtagccgc gctcgagcag gagctcggcg agatacgagc cgtcctgccc
     1321 cccgattccc gtgatgagag ctcgcttcac cgctccattg tcccaagagc ctcggcgtag
     1381 gcggcgagag tggcgtcggt gacgtctccc caggagaaga gcgtgcgtgc acgctcgcgg
     1441 cctgccagcc cgagtcgccg ccgcagctcc ttatccgcga ggagccggtc gagcgcggat
     1501 cgcagggcga ccgggtcgcg gggcggcacg acgatcccgg tctcgccgtc cacgaccaga
     1561 tccagcagcc cgccgacatg cgtcgcgacc acgggacggc cgtgcgccat cgcctcgagg
     1621 caggcgacgc caaagccttc gcgttgcgag ggacagacga ccacggcggc gcgcgcgtag
     1681 aggtgctgca gctcctcgtg ttggacgaaa cccctggcaa gtgggaaacg gtcgcgcaga
     1741 gggccgtcgc cggcgaccac caggttcagt ccctgcgcgg cgtcgagcag ctcgagcact
     1801 cccttctccg gcgagaggcg gcctgcataa agcacctcgg ccggctcggc ctcctcgccg
     1861 acttcgggag gaaggtcgac gccactcggg atcacgcgca cctcgcgcgc accgagcgat
     1921 cgagccgcgt ccgccagcgc cctcgaggcg gcgatgacga gccgagccct gcccagcact
     1981 ccccgtgcaa gccagggcgc gcggcgcgcg agctcgacgt ccgtccccca tacctgcacg
     2041 acatacggct tgccggtccg cgcggcgacc cagccggcgg cgagccagtg cgcgtgcagg
     2101 aggtctgcgt cggcgcgacg ggcggcgcga aggaagttcg tcaaaagagc cggcacgaga
     2161 accgcacgcc aggggcggcg gcgcagattg ccgacgatcc catgtccgta ggcaatcccg
     2221 aaatgaccga actgctcggg gccgatcacc gtgaccgcga ctccccgggc ccgcacacgc
     2281 tcgaccgcat cggcgacgaa tcgtcctgcg ccgtgatccc gatcgctcgg gtacgaggtc
     2341 gtgaggacgg cgaccttcac cgggttgtcc ctatggcttt gattgctcga gccgagggtt
     2401 gaacccggcg gtgcgggctt ctgcgatgaa atcgcggcca acttgcaatg tcggaatgcc
     2461 gtgcagagtc actttcagga gcccacaacc gtcggattcg atctgcgtgc ctttgaagcc
     2521 gacctccaac gcacgccgca gtaccgcgtc ggcgcgagca ctagtcgcga accggccgaa
     2581 gaccgcctcg aggttgccgt catgcttcgc cggcctgatg cagtccgcgc gactggggaa
     2641 gctcggacga ccatgcgcca aggctccgag ggggtaggcg accgcggcaa tcagcaggat
     2701 cgcccacgga aggattcggc ttctcacgac gcaccatccg aacgcaagcc acgccacgcg
     2761 gcggagagga gcagcgggac gagcgcggtc gaggcaaggg ccagcgaacc ggtgagccgg
     2821 atgatcgggt tcacgttggg cgccttggtg at
//
LOCUS       DASVEU010000147         9406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1070672,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000147 DASVEU010000000
VERSION     DASVEU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070672"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF225_05810"
     CDS             complement(<1..360)
                     /locus_tag="VF225_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7144808.1"
                     /translation="MTDAKLPGLEERFAEVKGVRMRYFLGGDGPPLILVHGLGGAAAN
                     WAELVPLLVRRHTLLVPDLPGHGGSTALPAVAGLEPFADRVALVAEREGMLPAPVVGH
                     SLGGMVVLRMALRQPDDV"
     gene            504..674
                     /locus_tag="VF225_05815"
     CDS             504..674
                     /locus_tag="VF225_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144809.1"
                     /translation="MANHLTPEELSKELGIDRQEVIRVCVQEGVPIYQGKIDKTLFQA
                     QLEALGAMPAHH"
     gene            complement(685..1566)
                     /locus_tag="VF225_05820"
     CDS             complement(685..1566)
                     /locus_tag="VF225_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7144810.1"
                     /translation="MRTIWNGSISFGLVSIPVGLALATTPKAKQSDVSFRTLHRECKT
                     PIRQKRWCPTHEREVGPDEIVKGWEVAKGEFVIVEDADLEAIAQHDESRSIEITRFVP
                     LDEVDPVYLDRTYYLAPAQAPAQRRPYVLLLEAMKQAGMGGIGRFVLRGAEYFALIRP
                     KGDALVLETLFLAEDVRSQAEIDEAVAGTEVKKAELDLAQQVMQSLVGEFDPSELHSQ
                     YRSDLRQMLEAKLDGQEIAKPEPVPDAPVLDLMEALKRSVAEAKDRKTTKSAASKTKA
                     APKSGSGGRRTPARKSA"
     gene            1767..3476
                     /locus_tag="VF225_05825"
     CDS             1767..3476
                     /locus_tag="VF225_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7144811.1"
                     /translation="MATATTTTGTRTIARLWQDAVARGLESPAYLVQEGEEWRPVSWT
                     EAAQAVDEIAHGLLALGVRKGDAFAILASTRLEWVLFDFALGLIGAVGAPIYMNNSPR
                     DAVYVAEHSEAVGVLCEDDEQRAKLAGLDLEHVLTFSDLPALRERGRAHAEEHPRAVE
                     EAASAIDENDLFTFIYTSGTTGPPKACMILHRNYYAMVDEVRQVQDFTVTDDVMLLYL
                     PLAHNFGRCLTLLGAHIGYTIAFCPDPYAVGDALPAVRPTVFPSVPRVYEKVHTAVTA
                     KFDEATGAKRRLIDWALDVGGKVSKLREAGRSVPPLLALQHKVAGRLVYAKVKSRLGG
                     KLRIGVSGGAPLAKEIIEFFAALDVIILEGYGLTECTTGATINRPTRYRFGSVGPALP
                     GVELRIADDGEVLIKTDTVFAGYFKDEEATREVLSEDGWLRSGDVGQLDEEGFLTITD
                     RLKDILVTAGGKNVAPQNLENALKTHAVISQALVVGDRRPYVAALITLSEDVDPAGAA
                     PAVQRAVDAVNRELSRYEQIKRFTILPRDFTLEAGEVTPTLKLKRRVCLEHFAAEIEA
                     LYS"
     gene            3554..4210
                     /locus_tag="VF225_05830"
     CDS             3554..4210
                     /locus_tag="VF225_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144812.1"
                     /translation="MGLTRGWRSTLVGAGAATLALAAIVAALGPALTVIPIEVLRLVV
                     GGLLLVFGLQWLRKAILRASGWRAVRDEAAVFARERAEAANAPRVERAGLDWYAFTIS
                     FKGVLLEGLEVAFIVLTFGSTQGSIPLAAVGAGAAVVLVVLAGIVVREPLSRVPENTL
                     AFAVGVMLTTFGIFWGAEGAGVDWPGGDASLPGVLGFVVLISLGFVTALRRRRKLAIA
                     "
     gene            4207..4392
                     /locus_tag="VF225_05835"
     CDS             4207..4392
                     /locus_tag="VF225_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144813.1"
                     /translation="MKRFGKFWWDFIVGDDWRVAVGILLAFGLTALLATTSVPAWWVL
                     PLAVAAVLWLSLRRVAR"
     gene            complement(4389..5534)
                     /locus_tag="VF225_05840"
     CDS             complement(4389..5534)
                     /locus_tag="VF225_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7144814.1"
                     /translation="MTYRPPPVRPSTRIFRVLVPSGRKRQAQAFILCIATLCAAASAN
                     ATPGTVASKQAQAQQVLAQIQTIDDSLGVAVESYNLANVRLQKIESDQRENRLQLKVT
                     RANLRIAQDSLAARLVSAYTSTQDNSTLSVLLGATSLDDLLNRIEAVNSTSQQDASIV
                     QQVTSFKAAIQRHRIELQQAHSEQRTIVAQKAAQKQRIESQLASRRQLLSSIKGQIER
                     IRAAEAAQQRQLAAAARSRLSGGTPISLPDGVGISASTPEGSTVAPPNVHGGVVGIAM
                     HYLGVRYVWGGSTPSGFDCSGLVSYVFAQIGVSLPHSSYAQFGMGVSVSISELQPGDL
                     VFFTGASHVGIYIGSGQFIHAPHTGDVVKISSLSGYYSSNFAGGRRI"
     gene            5654..6670
                     /locus_tag="VF225_05845"
     CDS             5654..6670
                     /locus_tag="VF225_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX7144815.1"
                     /translation="MRWPRESRFALGLALGAALLLGVFLPGAEGADSKKGAQSSPRQQ
                     RTGLAGRSHTALLSLYALDSRLLQARSELAGLRSRADGLRLERDRVQQEIAVVAGNLK
                     ASQRLLGDRLRALYEEGEPDAIAVLLGAKSLDDAVTRLDELERGARQGAQAATDTRDG
                     RSKLQGLALELAARIRAVQALEAQTVQTAATLRSARAKRVSYLAALSRQRRLNARQIR
                     ALDHRARQVVAKAETVQAQTAPTSSAGAPWVVVGARTLTVTATGYSLPGRTSTGLPVG
                     FGVIAVDPGVIPLGTRISIPGYGEGVAADTGSAVQGMTIDLWFPTLAQALGWGRRTVT
                     ITLH"
     gene            6707..7387
                     /locus_tag="VF225_05850"
     CDS             6707..7387
                     /locus_tag="VF225_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144816.1"
                     /translation="MSQLVQADDQALGQVRVLIVDDHDLFRSGLRNLLEDEGVHVVGE
                     AAAGQEALTIVREVAPDVVVMDLNMPGMGGVEATRHISTIAPLTRVVMLTISDQDNDV
                     IDAILAGACGYLLKDSSIQELIAGIKAASMGESLISPTIASKVLQRVRASSTQPEIET
                     TIRAELSEREIEVLKLIANGKDNAMIAGELHISPKTVKNHISNILMKLQIDNRIQAAV
                     YAVRSGIV"
     gene            complement(7384..7713)
                     /locus_tag="VF225_05855"
     CDS             complement(7384..7713)
                     /locus_tag="VF225_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144817.1"
                     /translation="MIHDEIRSLLDAPPQGDEAPTIDAIEHTLTAGYARALALEAERW
                     RLERRIAEVAATLGGKSPDDEHSELTQLGRRLSAADGDLTNLRVLLSSLRSLAEEVRQ
                     PSGQSSN"
     gene            7855..9069
                     /gene="dacB"
                     /locus_tag="VF225_05860"
     CDS             7855..9069
                     /gene="dacB"
                     /locus_tag="VF225_05860"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX7144818.1"
                     /translation="MNRRLVAALIALIALAAPAATAAGRAQDSQLQRRLARALHVPHV
                     ATARSAAVALDLASGTVLFEQNPGRSLAPASNEKLPLTYAALMQLGPTYRIETDVLGQ
                     GQQDGTQWTGTLVLKGNGDPTLSRADLRALAAQVKALGIRSVTGGVSGDESAYDTRRI
                     VAGWKPSFFIDESPPLSALVVDRARVGRIVTRSPALAAATAFRAALRQAGIAVAGPVR
                     TGTADAWSEPLADVSSPTLWTMLRFMDRESDNFTAEMLLKQLGLTELGRGTSAAGAAV
                     VTQALAEAGVPMTGVRIVDGSGLSQLDRLTANALGSLLKIAWTDPTVGPALLASLPVA
                     GVNGTLQHRLSKPPARGRVLAKTGTTDTASSLSGYVSDRYAFAVVQNGHPLSYWWARR
                     AQDRFAQILAGS"
     gene            complement(9202..>9406)
                     /locus_tag="VF225_05865"
     CDS             complement(9202..>9406)
                     /locus_tag="VF225_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX7144819.1"
                     /translation="GTLEGFTRDEAAAALEAKGAKVVNSVSGKTTGLVVGEEPGNSKL
                     TKARKVEVPILSEADLVELLQRA"
BASE COUNT         1472 a         3167 c         3201 g         1566 t
ORIGIN      
        1 aacgtcgtcc ggttgacgga gcgccatgcg tagcacgacc attcctccga gcgaatgacc
       61 gaccaccggc gcgggcagca tgccttcgcg ctcggccacg agcgccaccc gatcggcgaa
      121 cggctcgagc ccggcaaccg ccggcagggc ggtcgagccg ccgtgcccgg gcaggtcggg
      181 gaccagcagc gtgtgccgcc tcaccaggag cggaacgagc tcggcccagt tcgccgcggc
      241 gcccccgaga ccatggacga gaatgagcgg aggcccgtcc ccgcccagga aataccgcat
      301 tcggacgccc ttgacctcgg cgaagcgctc ctccaaacca ggcagttttg cgtctgtcat
      361 gccggccgat tatcctttgg accaatgact gctttgggag tgccggcccg ttgaatccgg
      421 caacgacata tggaggctcg acttagcccg ctgagcgcat ttgcgcaggc gggctttttc
      481 gtacgacaga gagaggagcg tcgatggcga accaccttac gcccgaggag ttgtccaagg
      541 agctcgggat cgaccgccaa gaggtcatcc gcgtctgcgt ccaggaaggc gtgcccatct
      601 atcagggcaa gatcgacaag acgctgttcc aggcgcagct agaagccttg ggcgcgatgc
      661 ccgcgcatca ctagggaacg aacgtcaggc agatttgcgc gccggcgtac gccggccgcc
      721 ggagccgctc ttcggggcgg ctttggtctt cgaggcggcg gatttcgtcg tcttgcggtc
      781 cttggcctcg gcaacactgc gcttcagcgc ttccatcagg tccaggaccg gcgcgtccgg
      841 cacgggctcg ggcttcgcaa tttcctggcc gtccagcttc gcctcgagca tctggcgtag
      901 gtcgctccgg tactggctgt ggagctcgga cgggtcgaac tccccgacga ggctctgcat
      961 cacctgctgg gcgagatcga gctcggcttt cttcacctcg gtgccggcga ccgcctcgtc
     1021 gatctcagcc tgcgagcgca cgtcctccgc gaggaagagc gtctcgagca ccagggcgtc
     1081 gcccttcggg cggatgagcg cgaagtactc tgcgccccgg agaacgaagc ggccgatccc
     1141 tcccatgccc gcctgcttca tcgcctcgag cagcagcacg taaggccggc gctgcgcggg
     1201 cgcctgagca ggggcgaggt agtacgtgcg gtccagatag accggatcga cctcgtcgag
     1261 cgggacgaac cgcgtgatct cgatggagcg ggactcgtcg tgctgcgcga tcgcctcgag
     1321 gtcggcgtcc tcgacgatga cgaactcgcc cttggctacc tcccagccct tgacgatctc
     1381 gtcaggcccg acctcacgct cgtgggtagg gcaccagcgc ttctggcgga tgggggtctt
     1441 gcactcccgg tggagcgtcc gaaagctcac gtccgactgc ttcgccttcg gcgtggtcgc
     1501 gagggccagg ccaacgggga tgctgacgag gccgaaagag atggatccgt tccagatagt
     1561 gcgcatggcc gccttgtaat tgtagaggga ccgggctgat tctccgtcgg tctggacttc
     1621 cctgcaaggc gccgactcgg cggcgccgcc ctagaggcca tacgggctcc gcccgtgtgg
     1681 ccgtctccaa ggcgccgact cggcggcgcc gccctagagg ccagacgggc tccgcccgtg
     1741 tggccgtctg aaattacgat ggaggcgtgg caaccgcgac aacgactacc gggacacgga
     1801 cgatcgcccg cctctggcag gacgccgtgg cccggggcct cgagagccct gcctatctcg
     1861 tccaggaggg agaggaatgg cgaccggtct cctggacgga ggcggcgcag gcggtcgacg
     1921 agatcgccca cggcctcctc gcccttgggg tccggaaggg tgatgccttc gcgatcctgg
     1981 cctcgacccg cctcgagtgg gtgctcttcg atttcgcgct cgggctgatc ggcgccgtgg
     2041 gtgcaccgat ctacatgaac aactcgccga gagacgcggt gtacgtggcc gagcactcgg
     2101 aggccgtcgg ggtgttgtgc gaggacgacg agcagcgcgc gaagctcgcc ggactggacc
     2161 tcgagcacgt gctgacgttc tccgatctcc ccgcgctccg agaacgggga cgcgcccacg
     2221 cggaagagca tcccagagcg gtcgaagagg cagcctctgc gatcgacgaa aacgatctgt
     2281 tcaccttcat ctacacgtcg ggcaccaccg gcccgccgaa ggcctgcatg atcctgcacc
     2341 gcaactacta cgcgatggtc gacgaggtgc ggcaggtgca ggacttcacc gtcaccgacg
     2401 acgtgatgct gctgtacctc ccgctcgcac acaacttcgg gcgttgcctc acgctgctcg
     2461 gcgcgcacat cggctacacg atcgcgttct gccccgatcc ttatgcggtc ggggacgcgc
     2521 tccccgctgt tcgtccgacc gtcttcccaa gcgtgcctcg ggtgtacgag aaggtgcaca
     2581 cggcagtcac ggccaagttc gacgaggcca ccggcgcgaa gcggcgactg atcgactggg
     2641 ccctagacgt cgggggcaag gtcagcaagc tgcgcgaggc ggggcgctcc gtgccacccc
     2701 tgctcgcgct gcagcacaag gtcgccgggc ggctcgtcta cgccaaggtg aagtcccgtc
     2761 tcggcggcaa gctccgcatc ggcgtgtccg gcggcgcgcc gcttgcgaag gagatcatcg
     2821 agttcttcgc agcgctcgac gtcatcatcc tcgagggcta tggcctgacc gagtgcacga
     2881 ccggtgcgac gatcaaccgc ccgactcgtt atcgcttcgg gagcgtcggg ccggcgctgc
     2941 ccggcgtgga gctccgtatc gcggacgacg gcgaggtcct gatcaagacc gacacggtct
     3001 tcgccggtta cttcaaggac gaggaggcga cgcgcgaggt cctctcggag gacggctggc
     3061 tgcgctccgg cgacgtgggc cagctcgatg aggaaggttt cctgacgatc accgaccggt
     3121 tgaaggacat cctcgtcacc gcgggcggga agaatgtggc gccgcaaaat ctggagaatg
     3181 cgctcaagac gcacgcggtc atctcgcagg cgctcgtggt cggcgaccgg cggccctacg
     3241 tcgcggcgct gatcacgctc tcggaggacg tcgatcccgc gggcgcggcg cccgccgtcc
     3301 agcgtgctgt cgacgcggtc aacagggagc tttcgcggta cgagcagatc aagcggttca
     3361 cgatcctgcc tcgcgacttc acgctcgaag ccggcgaggt gacgccgact ctgaagctga
     3421 agcgtcgtgt gtgcctcgag catttcgctg ccgagataga ggcgctctac tcctgacaga
     3481 tgtctttctc gttctgtccg cgttcctcgc gagcgcggtc gaaacggtcg aggctctcac
     3541 gatcgtgctt gccatgggcc tcacccgcgg ctggcgatcc acgctcgtgg gggcgggagc
     3601 tgcgacgcta gcgctcgcgg caatcgtcgc ggcgctcggg cctgcgctca ccgtgatccc
     3661 gatcgaggtg ctgcggctcg tggtcggagg gctgctgctc gttttcggcc tgcaatggct
     3721 gcgcaaggcg attctgcggg caagcggctg gcgggcggtg cgcgacgagg ccgcggtctt
     3781 tgcacgtgag cgcgccgaag ccgcgaacgc accgcgcgtg gaacgtgccg ggctcgactg
     3841 gtacgcgttc acgatctcct tcaagggcgt actcctcgaa ggtctggagg tcgcgttcat
     3901 cgtgctcacc ttcggaagca cgcagggaag cattccgctc gccgccgtgg gcgccggcgc
     3961 agctgtggtg ctcgtggtcc tcgcagggat cgtcgtgcgc gagccgctca gccgcgttcc
     4021 cgagaacaca ctcgctttcg cggtcggcgt gatgctgacg accttcggga tcttctgggg
     4081 cgcggaggga gccggcgtcg actggccggg cggcgacgcg tcgctgccgg gagtgctcgg
     4141 cttcgtggtg ctgatctccc tcggcttcgt caccgcgctc cgtcgccgcc ggaagctcgc
     4201 gatcgcatga aacggttcgg gaagttctgg tgggacttca tcgtcggcga cgactggcgc
     4261 gtcgccgtcg gcatcctgct cgcgttcggg ctcacggcgc tgctggcgac aacatccgtg
     4321 cctgcgtggt gggtgctgcc gcttgcagtc gccgcagtgt tgtggctgtc tcttcgccgg
     4381 gtggcgcgct agattcgccg gccgccggcg aagttcgacg agtagtagcc gctgagcgag
     4441 gagatcttca ccacgtcacc tgtgtgcggc gcatggatga actgcccgct gccgatgtag
     4501 atccctacat ggctcgcgcc tgtgaagaac acgaggtcgc cgggctggag ctcgctgatc
     4561 gagacggaga cgcccatccc aaactgcgcg tacgagctgt gcggcagcga gacgccgatc
     4621 tgtgcgaaaa cgtacgagac gagcccggag cagtcgaagc cgctcggcgt cgagccgccc
     4681 cagacgtaac gcacgccgag gtagtgcatc gcgatcccga caacgccgcc gtggacgttc
     4741 gggggcgcaa cagtcgaacc ctcgggcgtc gaggccgaga tgccgacgcc gtcgggcagc
     4801 gaaatcgggg ttccgcctga gaggcgagac cgggccgccg ccgcgagctg ccgctgctgg
     4861 gcggcctctg ctgcgcggat gcgctcgatc tggcccttga tcgacgagag gagctgccgg
     4921 cgcgaagcca gctgcgattc gatccgctgc ttctgcgcgg ccttctgcgc cacgatcgtc
     4981 cgctgctccg aatgggcctg ctgcaactcg atccgatggc gctggattgc cgccttgaag
     5041 gacgtcacct gctggacgat gctcgcgtcc tgctgggagg tcgagttgac cgcctcgatg
     5101 cgattgagga gatcgtcgag gctcgtcgca cccaggagca ccgagagagt cgagttgtcc
     5161 tgggtcgagg tgtaggcgct cacgagccgg gccgccagcg agtcctgcgc gatcctgagg
     5221 ttcgcccgcg tgaccttcaa ctgcagccgg ttctcgcgct ggtcgctctc gatcttctgg
     5281 aggcggacgt tggcgaggtt gtacgactcg acggccacgc cgagactgtc gtcgatcgtc
     5341 tggatctggg ccaacacctg ctgtgcctgc gcctgcttgg aggcgaccgt cccgggcgtg
     5401 gcgttggccg atgccgccgc acacagcgtt gctatgcaaa ggatgaacgc ctgcgcctgt
     5461 cgtttcctgc cgctgggcac cagcacgcgg aagatccggg tcgacgggcg caccggaggg
     5521 ggccggtacg tcacaagtgg cgcaccctag cagcgtcttc ggatgtctgc aaccgctttc
     5581 cgagcctaaa agtgatcatt cctcccatgt gtgagccccg cacaaagtcc tgagcttgct
     5641 agggtcacag gccttgcgtt ggcctaggga gagccgcttc gcgctcgggc tggcgctcgg
     5701 agccgcgctg ctcttgggag tctttctccc tggggctgag ggcgcagact cgaagaaggg
     5761 agcccagtct tctccgcgcc agcagcggac gggactggcc ggccgctccc atacggccct
     5821 tctgagcctc tacgccctgg attcgcgcct tctccaggcc cggagtgagc tggcaggcct
     5881 gcggagccgc gcggacggcc tcaggctcga gcgggaccgg gtccagcaag agatcgcggt
     5941 cgtcgcgggc aacctcaagg catcccagcg cctcctgggc gaccgtctga gggccctcta
     6001 cgaggaagga gagcccgatg cgattgccgt cctgctcggc gccaagtcgc ttgacgacgc
     6061 cgtgacgcgc ctcgacgagc tcgagcgggg cgcccgccag ggcgcccagg ccgcgacgga
     6121 caccagggac ggccgctcga agctgcaggg cctcgctctg gagctcgccg cccgcatccg
     6181 ggcggttcag gccctcgagg cccagacagt gcagaccgcc gcaacactcc ggagcgcgcg
     6241 ggccaagcgg gtctcgtatc tggctgcgct ctcgcggcag cggcgcctga acgcccggca
     6301 gatccgggcg ctcgatcatc gggcccggca ggtcgtggcc aaagcggaga cggtgcaggc
     6361 gcagaccgcg ccgacatcct cggccggcgc cccctgggtc gtcgtcggcg cccgaacgtt
     6421 gacggtcacc gcgaccggct actccttgcc gggccggacg tcgacggggc tccctgtcgg
     6481 cttcggggtc atcgcagtcg atccaggcgt catcccgctc ggcacccgga tctccatccc
     6541 cggctacggc gaaggggtag ccgccgacac gggcagcgca gtccagggca tgacgatcga
     6601 cctgtggttc ccgactctgg cccaggccct gggttggggc cgccgcacgg ttacgatcac
     6661 cctccactag acttcgtccg cccgcggcca gggaaggacg cccagcatga gtcaactcgt
     6721 acaagcggac gatcaggcgc tgggccaggt ccgcgtcctg atcgtcgatg accacgatct
     6781 cttcaggagt gggctacgca acctcctgga agacgagggc gtgcacgtgg tcggggaggc
     6841 tgcggccgga caggaggcgc tgaccattgt ccgcgaggtg gcgcccgacg tcgtcgtgat
     6901 ggatctgaac atgcccggca tgggcggcgt cgaagcgaca cgccacatct cgacgatcgc
     6961 accgctcact cgggtcgtga tgctgacgat ctcagatcag gacaacgacg tgatcgacgc
     7021 gatcctcgcc ggcgcgtgcg gctatctgct caaggactcg tcgattcagg agctgatcgc
     7081 cggaatcaag gcagcgtcga tgggcgagtc gttgatctcg ccgacgattg cctcgaaggt
     7141 tttgcagcgt gttcgtgcgt ccagcactca gccggagata gagaccacaa tccgggccga
     7201 gctttctgag cgcgagatcg aagtgctgaa gctgatcgcc aacggcaagg acaacgcgat
     7261 gatcgccggc gagctccaca tcagtccgaa gaccgtgaag aaccacatct cgaacatcct
     7321 catgaagctg cagatcgaca accgcatcca ggcggccgtc tacgcggtga gaagcgggat
     7381 cgtctagtta gacgattgac cagacggctg gcggacctct tccgcgaggc tgcgcagcga
     7441 ggacagtagg acgcgaaggt tggtgagatc gccgtcggcg gcggacagcc gtcgcccgag
     7501 ctgggtgagc tcggaatgct cgtcgtcagg cgacttgcca ccgagcgtcg cagcgacctc
     7561 cgcgatccgg cgctcgagcc gccagcgctc ggcctccaac gcgagcgctc tggcataccc
     7621 ggccgtgagg gtgtgctcga tggcgtcgat ggtcggcgcc tcgtcgcctt gcggcggggc
     7681 gtcgagaagc gagcggatct cgtcgtggat catgcggagt tcgacctcga tttccgggat
     7741 ccggaccagt atcgagcctg agcccacaac gcacatgggc cgcccggact atcttctgcc
     7801 ctgcaccggc ccatcttctt gactccatct gctggcccga aaaatggatt ccgcgtgaac
     7861 cgccgtcttg tcgcagccct catcgcgctg attgccctgg ccgcacctgc cgcgactgcg
     7921 gcagggcggg cgcaggactc gcagctgcaa cgccggctcg cccgcgcgct ccacgtcccc
     7981 cacgtcgcca cggcgcgatc ggcggccgtc gctctcgatc tcgcttccgg caccgtcctc
     8041 ttcgagcaga accccggccg gtcgctggct ccggcgtcga acgagaagct gcccctcacc
     8101 tacgcagcgc tgatgcagct cggtcccacc tatcggatcg agaccgacgt gctcggtcag
     8161 ggccagcagg acggaacgca gtggacgggc accctcgtcc tgaaagggaa cggagatccg
     8221 acgctgtcgc gcgccgactt gcgcgcgctc gctgcacagg tgaaggcctt gggaatccgc
     8281 agcgtgacgg gcggcgtgag cggcgacgaa agcgcctacg acacgcggcg catcgttgcc
     8341 gggtggaagc cgagcttctt catcgacgag tcgccgccgc tttcggcgct cgtcgtcgac
     8401 cgcgcccgcg tcggccgcat cgtcacgcgc tcgccggcgc tcgcggccgc gacggcgttc
     8461 cgtgccgcgc tgcggcaagc cgggatcgcc gtcgccgggc ccgtgcggac cggaacggcc
     8521 gacgcgtggt cggaaccgct tgccgacgtg tcgtcgccga cgctctggac gatgttgcgc
     8581 ttcatggacc gcgagagcga caacttcacc gccgagatgc tgttgaagca actggggctc
     8641 acggagctcg gccgggggac gagcgcggcg ggagcggccg tggtcacgca ggcgctggcc
     8701 gaagcgggcg tgccgatgac gggtgtgcgc atcgtcgacg ggtctggact gtcgcaactc
     8761 gaccgcctga cggcgaatgc gctcggcagc ctcttgaaga tcgcctggac cgatccgacc
     8821 gtcggcccgg ccctactcgc gtcgctcccg gtggcgggcg tgaacggaac gctgcagcac
     8881 cgtctaagca agccgcccgc ccgagggcgc gtcctggcga agaccggcac gacggacacc
     8941 gcgtcgtcgc tgtccggcta cgtcagcgac cgctacgcgt tcgcagtggt gcagaacggg
     9001 catcccctgt cgtactggtg ggcccgccgc gcccaggacc gcttcgccca aatcctggcc
     9061 ggttcgtagt cacaaagccg acacgctttc gtgacatcga cgtctgcttt tcgggtccga
     9121 ccccacacgt gtcgcaccgg gacatgtcgg accggtccga cgcggtcggt ttccgcgtca
     9181 gacggcgcaa tttcgtttcg cttacgcgcg ctgcaggagc tcgaccaggt cggcttcgga
     9241 caggatcggg acttcgacct tgcgggcctt cgtcagcttc gagttgcccg gctcctcgcc
     9301 tacgaccagt cccgtcgtct tgccggaaac cgagttcacg accttcgcgc ctttcgcctc
     9361 cagcgccgcg gccgcctcgt cgcgcgtgaa gccctccaac gtgccg
//
LOCUS       DASVEU010000148         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_684617,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000148 DASVEU010000000
VERSION     DASVEU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684617"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="VF225_05870"
     CDS             <1..2358
                     /locus_tag="VF225_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144820.1"
                     /translation="GRCLSYGTGTAYWALGEVLKEHLGLLESDPPELALDRLGGRAIL
                     GLTLGLDVARELHPLAARDRFQDAWAEFLSEVVAERPAVVLVEDIHWAEPQLLDLLEY
                     VLVSVQGPLLLMATARPELLQRRPGWGARAGGELLELEPLSAQESVQMLDEMLTGGLP
                     QELSELVVERAEGNPFYVEELLGVLIDGGLLSRRNGGWALRELPSDFAIPDSVHAVLA
                     ARIDLLSTAEKSALQAAAVIGRTFWAGPVYELVEGKPDLRVLEDRDFVRRRAGSSMAG
                     EREYVIKHALTREVAYESLPRALRARMHAAFAAWLERRGQGRDELASLLAHHYAEAAR
                     PEDADLAWPGEEAELERLRAKALLWLETAGEFATRRYEIEDALALLRRALSLRPDEEA
                     QARLWRSMGKANALKFDGEAFWTAMQNSLKVCSNKATCADTYGELAFQTAIRASMWPK
                     RPDRELVGGWIEQALELSEEESVSRARALIARSFWERNTSEAAREASELAERFGDLEL
                     RSYAWGARAAVAFAQADFESALSWSQRRLSVLDEISDPDHVADIYEIAIPSCCANARF
                     PEARRLAAEHDAIVAPLSAHHRVHGIAVLLEVEEVFGGWSRILELAERTTQAVEANLA
                     TPCVRNARSLLVTALAAAHAGDDETARRFERRADEVATEGYDFALAAPRIWLALSRGR
                     LGEADGLEPVDLARGPIWYALPAAAARLDALAARQERSPVERDAQALLRPGTYLEPFA
                     LRALGAVRQEEVLLKQAVDRFDAMGLTWHAEQTRELLARTSRNPV"
     gene            complement(2717..2789)
                     /locus_tag="VF225_05875"
     tRNA            complement(2717..2789)
                     /locus_tag="VF225_05875"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2754..2756),aa:Trp,seq:cca)
     gene            2821..2985
                     /locus_tag="VF225_05880"
     CDS             2821..2985
                     /locus_tag="VF225_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144821.1"
                     /translation="MRRRPLGALFLVLAAGFAAVAVYAALSGGAAWVVAVAAAALAVW
                     MGESALRALR"
     gene            3028..>3510
                     /locus_tag="VF225_05885"
     CDS             3028..>3510
                     /locus_tag="VF225_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144822.1"
                     /translation="MVERADDTQALWQQFKRTGDRALRDRLILTYAPLVKYVAGRLGS
                     GLPAHVEEGDLVSYGLLGLIGAIERFDPDRDIKFETYAIARIKGSIIDELRSMDWVPR
                     SVRARARDIERTIATLEAKHARAPTDEEIAHSLGITEEEFQDSLLEISRTSIAALDEL
                     W"
BASE COUNT          533 a         1159 c         1245 g          573 t
ORIGIN      
        1 ggccgctgcc tctcctacgg aaccgggacc gcgtattggg cgctggggga ggtgctgaag
       61 gagcacttgg gcctcctcga gagcgaccca ccagagctcg cgctcgaccg gctcggcggc
      121 cgggcgatcc tgggcctcac gctcggtctc gacgtcgccc gcgaactgca cccgctcgcc
      181 gcgcgcgatc gcttccagga tgcctgggcc gagttcctga gcgaggtcgt cgccgagcgc
      241 cccgcggtcg tgctcgtcga ggacattcat tgggctgagc cccaactcct cgacctgctc
      301 gagtacgtcc tcgtcagcgt gcaaggcccc ctgctcctga tggcgactgc gaggccggag
      361 ctcctccaac gtcgacccgg ctggggagcg cgcgccggcg gtgagctgct cgagttggaa
      421 cccctctcgg cccaagagtc tgtccagatg ctggacgaga tgctcacggg cggcctgccg
      481 caggagctta gcgagctcgt cgtcgagcgg gccgagggca acccgtttta cgtcgaggag
      541 ttgctcggcg tgttgatcga cggtggcctg ctctcccgcc ggaacggcgg ctgggcgctc
      601 cgtgagcttc cgagcgattt cgcgattccc gattcggtcc acgccgttct cgcggctcgc
      661 atcgacctgc tcagcacggc ggagaagtcg gcgctccagg ccgcggcagt catcgggcga
      721 accttttggg ccgggcccgt ctacgagctt gtcgagggaa agcccgatct gcgcgtgctc
      781 gaggatcgcg acttcgtccg ccgtcgggcc ggctcgagca tggccggtga gcgcgagtac
      841 gtgatcaagc acgcgctcac tcgcgaggtt gcgtacgaaa gccttccgag agctcttcgc
      901 gcacggatgc acgcggcgtt cgctgcctgg ctcgagcgcc gcgggcaagg ccgcgatgag
      961 cttgcgtcgc tgctcgcgca ccactacgcg gaggcggcgc gacccgagga cgcggatctc
     1021 gcctggccgg gagaagaggc agagctcgag cgtttgcgcg cgaaagcgct tctttggctc
     1081 gagaccgcgg gcgagttcgc gacgcggcgc tacgagatcg aggacgcgct ggcgctgcta
     1141 cgccgggcgc tctcgctgcg acccgacgag gaggcgcagg cgcggctctg gcgctccatg
     1201 ggaaaggcaa acgcgctcaa gttcgatggc gaggccttct ggaccgcgat gcagaactcg
     1261 ctgaaggtct gctcgaataa ggcgacgtgc gcggacacgt atggcgagct cgccttccag
     1321 accgcgatcc gtgcctcgat gtggccgaag cgacccgacc gggagctcgt cggcggttgg
     1381 atcgagcaag cgctagagct ctccgaggag gagagtgtgt cgcgcgcgag agcactcatt
     1441 gcgcgcagct tctgggaacg gaacacctcc gaggcggcac gcgaggcgag cgaactcgcg
     1501 gagcgtttcg gtgatctcga actgcggtcg tatgcgtggg gagcccgagc cgccgttgcc
     1561 ttcgcacaag cggacttcga gtcggctctg agctggtcgc aacggcggct gagcgttcta
     1621 gatgagatca gcgacccaga ccacgtcgcc gacatctacg agatcgcgat cccgtcatgc
     1681 tgcgccaatg cccgttttcc cgaggcgagg cgtctcgccg ccgagcacga cgcgatcgtc
     1741 gcgccgctct cggcacacca tcgtgtccac ggaatcgccg tcctgctcga ggtcgaggag
     1801 gtgtttggcg gctggagccg gatcctcgaa cttgcggaac ggacgacgca ggcggttgaa
     1861 gccaacctgg caacgccctg tgtgcgcaac gcgcggtcct tactcgtgac cgcgctcgcc
     1921 gcggcacacg cgggagacga cgagacggcg cggcgctttg agcgacgcgc cgacgaggta
     1981 gcgaccgaag gctacgactt cgcgcttgcg gcgcctcgaa tctggctggc actgtcgcgc
     2041 ggccggcttg gcgaggccga tggtctcgaa ccggttgacc tagcgcgcgg cccaatctgg
     2101 tacgccctgc ccgcggcggc agctcgcctc gacgcgctcg ccgctcgcca ggaacggtcg
     2161 ccggtcgaac gagacgccca ggcgctcttg cggccgggca cctatcttga acccttcgcg
     2221 ctccgcgcgc tcggcgccgt ccgccaggag gaggtgctcc tcaagcaggc ggtcgatcgt
     2281 ttcgatgcaa tgggactgac gtggcacgct gagcagaccc gggaactcct cgcacggacg
     2341 agcaggaatc cggtgtgatg agttcttggc gcggttctgc cgcatagcgc ggcgggcggc
     2401 cgaacttcta tgtgtcgtag gcgtcggccg caaagtcccg tcgttcaact cctgggtgcc
     2461 gcggcagacg ggcattcccc gaggcccatg gcggttatga gggcggccat gtctgcccgg
     2521 gcacttcttt gaccaccgaa ggtaccgctg cgggtgtggc tgcaggggca tgtaggagcc
     2581 taatcgtgcg ggccgattgc gggccactga gggccagtcc tgagcggatt ccaccggagc
     2641 caaccggaga gagtcggggc atccgtgaga cgaccttaga gcgcatggcc gctgagctgc
     2701 cggcgttcat gcggtacagg gccggcagga ctcgaacctg caacccccgg ttttggagac
     2761 cggtgctctg ccagttgagc tacggcccta cgcgtctcgg cgaggaggca ttgtatcggc
     2821 gtgcgtcgga ggcccctcgg agcgctcttc ctcgtcctcg cggccgggtt cgccgccgtc
     2881 gcggtctacg ccgcgctctc ggggggcgct gcgtgggtgg tcgcggtggc ggcggctgcc
     2941 ctcgcggtct ggatggggga gtcggcgctg cgggcgctcc ggtaggaaaa gcagcgattt
     3001 ctgacgaaag ccttatcctc cgtggcgatg gtcgagcggg ctgacgacac ccaagcgctc
     3061 tggcagcagt tcaagcgcac gggagaccga gcgcttcgcg accggctcat cctcacctac
     3121 gcgccgctcg tgaagtacgt ggcgggccgc ctcggcagcg gcctcccggc gcacgtcgag
     3181 gagggcgacc tcgtctcgta cgggctcctg ggcctgatcg gcgcaatcga gcgcttcgac
     3241 cccgaccgcg acatcaagtt cgagacgtac gcgatcgcgc gcatcaaggg ctcgatcatc
     3301 gacgagctcc gctcgatgga ctgggtgccg cgctccgtgc gcgcccgcgc gcgggacatc
     3361 gagcggacga tcgccacgct cgaggcgaag catgcgcgcg cgccgaccga cgaggagatc
     3421 gcacactcgc ttgggatcac ggaggaggag ttccaggact cgctgctcga gatctcgcgg
     3481 acgtcgatcg ccgcgctcga cgagctctgg
//
LOCUS       DASVEU010000149         7577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1072785,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000149 DASVEU010000000
VERSION     DASVEU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072785"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF225_05890"
     CDS             complement(<1..291)
                     /locus_tag="VF225_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144823.1"
                     /translation="MALSETVGFDPADPGFLADPYPVLNRLRERAPLFYDAGRERWFV
                     TRHEDVRSCLRDKRLGRNFRHLLSPEEIGVPPLDARWQAFWDSERWSLLWLEP"
     gene            413..1414
                     /locus_tag="VF225_05895"
     CDS             413..1414
                     /locus_tag="VF225_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7144824.1"
                     /translation="MLPLGLAALTAVILTAVGFVASSAAAPQATFKAGLVSDVGRFND
                     KGFNQSQLVGMKRAAKVLKIQYRAIESRAAAEYLPNMASLARSGYNIVISAGFLLADA
                     TEDVADQFPSTKFAITDQSVAALKRKHTNIEGLTYATQENSYLVGCLAAKAAAKRGTK
                     IISATAGLKIPPVDTFLAGYKAGANKCVPGTQVLIGYSGTFIDQAACKTIATNQIDAG
                     SQVVFAVAGPCGLGALDAAKERSRWGVGVDVDQSYLGSHILTSAVKRVDVGIFLAVKN
                     AKQGKYKGGKDVVYNLKNNGVALGKISKSVPKAWITQVNKLKAQIISGKIKPPKIVP"
     gene            1485..3008
                     /locus_tag="VF225_05900"
     CDS             1485..3008
                     /locus_tag="VF225_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144825.1"
                     /translation="MDEPPVLELRGITKRFPGVLANDHVDFDLRRGEVHALLGENGAG
                     KSTLMSILYGLYSADEGEILMNDEPVEISSPKAAIERGIGMVHQHFMLIPVMTVTENI
                     VLANEPVHAGVLFDEEAAEKRVAEVARTFNFAVDPHARVQNITVGQQQRVEIMKALYR
                     NADILILDEPTAVLTPQEAAELFEILRTLTREGISVIFITHKLNEVLEIADRITVLRR
                     GKRVETIPRAGATEAGLARSMVGREVLLRVEKKAATPGEPLLQVKDLVVRDDRGLDAV
                     RGVTFDVRAGEIVGIAGVDGNGQSELIDALTGLRHAASGSIQVAGEDVTSATARGTLD
                     AGIGHIPEDRQRRGLVLEFNLAENLVLHDYGKEPFSRFGLLNPRSLFGWARKLLQEFD
                     VRGGGPETRGASLSGGNQQKVVLAREINRDPRLLIAGQPTRGLDVGAIEFVHQRLVEQ
                     RDEGKAVLLVSLELEEILSLSDRILVLYEGRIVGEFTPSVSEEQLGIAMTGGRVEAA"
     gene            3028..4341
                     /locus_tag="VF225_05905"
     CDS             3028..4341
                     /locus_tag="VF225_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144826.1"
                     /translation="MSDSEREPPNGEGGAPAPLDVQPEGYAPSVAARLARYQRAGGII
                     TPFLTALVAFLMGGIVVLSTGHNPITTYKAIFEGSGLNWFFHFGSHQITIPGTDHQVW
                     FWWDTNTNHVAAYNLTQTLITTTPLILTGLAVAFAYRCGLFNIGGQGQYTVGAIVGVF
                     VGSRFVELAHFPHVLLVIVLASLAGAAWAAIAGFLKATVGAHEVITTIMLNWVAYWIG
                     SYLFGRGGPLQNHRDAAVPVSDDIVDKARLPVFWGNSHLQGLHVGLFIAIGMLFVFWF
                     ILNRTTTGYEVRAVGFNPEAARYGGISVSRNFVLAMAISGLFAGLAGAIDIMGWQFRL
                     GVLDVQVSNIGFIGIAVALLGRNTAVGVGFSALLFGALFTGTSTRSLDPSVFPPELAG
                     NLALMIQALILLFVGADVLILYLWQARRKVGLGRRAASSAAVEEA"
     gene            4338..5567
                     /locus_tag="VF225_05910"
     CDS             4338..5567
                     /locus_tag="VF225_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7144827.1"
                     /translation="MITAPTEGRALPLLTPRWIAIGGIALGVFAFWLSIPPISTRAPW
                     WPVLIGLCAIAAGVWAISRGQRRAGWGAVAAGLIGIALGLLATRASTGHLDQVVVWSA
                     LLTGMLVWATPLTFAAIGGMFCERSGVVNIGLEGMMLSGAFFGVLGADKFGSWGWGLV
                     TAMIAGGGFALVHAFFAIQLRADQIVGGTAINFLALGVTGYFFIKIYGANGTPGNLSR
                     IPDLDRHTAVVGPIFRGIDGIPFVGDVLGQLNLMIWLSFLILVVAHIVLFKTPIGLRL
                     RAVGEHPRAADTVGVSVYGVRYAATVLSGMLAALGGAYLAIGYVGSFSENMTAGRGFI
                     ALAAVIFGNWRPFGAFGAALLFGGSTALSFRLADAWGNSAVLFQALPYVLTLIAVAGV
                     IGRSVPPAASGRPYIKQ"
     gene            5617..5892
                     /locus_tag="VF225_05915"
     CDS             5617..5892
                     /locus_tag="VF225_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144828.1"
                     /translation="MPAAIEVAARSKRIGLLDAAYAVPVAFLLGLVALIMARRARRNL
                     RWLQLREGGTSVASAAVLVAAVTVCLALAAALSVGFYGLVVLYQHSR"
     gene            5941..6663
                     /locus_tag="VF225_05920"
     CDS             5941..6663
                     /locus_tag="VF225_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7144829.1"
                     /translation="MFEIGNSLREARLRQSLDFPEIEQGTKIRGKYLRALEEEHFDVL
                     PAQTYVKGFLRSYAEYLGLDGQLYVDEYNSRFVVGEEEHPQSRPRRSAPPSRGVQVQS
                     RVVLLTLLGITAVTALVIVAWTRGEPPSSTPVGLGTSKPPTHKTAAPPVTRALQVHLL
                     VRASRGPCWLQVHKTSATGPILFQGTLDQGQKQLFTARKLWITLDRPENLVTVLNGHT
                     RRLPVGGVKTLIVTPRAIRPAV"
     gene            6660..>7577
                     /locus_tag="VF225_05925"
     CDS             6660..>7577
                     /locus_tag="VF225_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7144830.1"
                     /translation="MTTRPRGFIVVTGSELVRGERRDLNGPFLATELLRHGVEPARIS
                     IVGDREEELSDALAHALGAADLCVVSGGLGPTHDDRTVELVARAAGVGLRLDEELYEQ
                     IGGVSRRFAARLGRPYADFEAGVRKQATIPEGAHSLGLAGTAPGLVLENGNTVVVVLP
                     GPPAELQRLWETALESEPVRRVLARGRPPELRQLRFFGASESTVAKALADAGGDGDGV
                     EATICARDFEIHVDFVVEPGAEARADELTRELVAPLERYLFSRDEHPVEELVLDLCRS
                     RGLTLGAAESCTGGMVAERLTSVPGSSDVF"
BASE COUNT         1206 a         2527 c         2546 g         1298 t
ORIGIN      
        1 tggctcgagc caaaggaggc tccaacgctc cgagtcccag aacgcctgcc agcgcgcgtc
       61 caacggcggc actccgatct cttccggcga caggaggtga cggaagttgc gtccgagccg
      121 cttgtcgcgc aagcagctgc gaacatcctc gtgccgggtg acgaaccagc gctcccggcc
      181 ggcgtcgtag aagagcgggg cccgttcgcg caggcggttc agcaccggat acgggtccgc
      241 cagaaacccg ggatcagctg ggtcgaaccc gaccgtctcg ctcagcgcca cggcctaacc
      301 ataggcgccg tccgccgtct tacagatgaa accagggacg gcaggcagta gcatcgcccc
      361 tgatgaatag gtgggtctcg acgaggagga gagtcttttg aagaagcatc gaatgctgcc
      421 gcttggcctg gcggcgctca ctgccgtgat cctcacggcc gtggggtttg tggccagcag
      481 tgctgcggca ccacaggcga cgttcaaggc cgggctggtc agtgacgtcg gccgcttcaa
      541 cgacaagggc ttcaatcagt cccagctggt gggaatgaag cgggcggcga aagttctgaa
      601 aatccagtac cgcgcaatcg aatctcgcgc ggcggcggag tacctgccga acatggcttc
      661 gctggcgcga agcggctaca acatcgtcat ctcggccggc ttcctactgg cggatgcgac
      721 cgaggatgtg gccgatcagt tcccgagcac gaaattcgcg atcaccgatc agtcggtcgc
      781 ggctctgaag agaaagcaca cgaacatcga gggattgacg tacgcgacgc aggagaacag
      841 ctacctcgtc ggctgcctcg cggcgaaggc ggccgcgaag cgcggtacga agatcatcag
      901 tgcgacggcg ggtttgaaga tcccgccggt ggataccttc ctcgccggct acaaggcggg
      961 cgcgaacaag tgcgtcccag ggacacaggt gctcatcggc tattccggca cgttcatcga
     1021 ccaggccgcg tgcaagacga tcgcgaccaa ccagatcgat gccggctcac aggtcgtctt
     1081 cgccgtcgca ggcccgtgcg gcctcggcgc gctggacgcg gcgaaggagc gcagtcgctg
     1141 gggcgtcgga gtcgacgtcg accagtcgta cctcggcagt catatcctga cgagcgcggt
     1201 gaagagggtg gacgtgggca tcttcctcgc ggtcaaaaac gcgaagcagg gcaagtacaa
     1261 gggcggcaag gacgtcgtct acaacctcaa gaacaacgga gtggccctcg gcaagatcag
     1321 caagtcggtg ccgaaggcct ggatcacgca ggtgaacaag ctgaaggcgc agatcatctc
     1381 gggcaagatc aagccgccga agatcgttcc gtaggacgca aagtgtgccg tgagagcggg
     1441 cctccgggcc cgctctctct ttctcggctt agaatcgcct ccggatggac gagccccccg
     1501 tcctcgagct acgcgggatc acgaagcgct tccctggagt gctcgcgaac gaccatgtcg
     1561 acttcgacct gcggcgcggc gaggtgcacg cgctcctcgg cgaaaacggc gccgggaagt
     1621 cgacgctcat gagcatcctc tacggcctct actcggccga cgagggcgag atcctgatga
     1681 acgacgagcc cgtcgagatc agctcgccca aggccgcgat cgagcgtggg atcggaatgg
     1741 tgcaccagca cttcatgctg atccccgtca tgacggtcac cgagaacatc gtcctggcga
     1801 acgagccggt gcacgcggga gtcctgttcg acgaagaggc agcggagaaa cgcgttgccg
     1861 aggtcgcgcg gaccttcaac ttcgcggtcg atcctcatgc ccgggttcag aacatcacgg
     1921 tcggccagca gcagcgcgtg gagatcatga aggcgctgta ccgcaacgcc gacatcctca
     1981 tcctcgacga gccgaccgcc gtgctcacgc cacaggaggc cgctgagctc ttcgagattc
     2041 tcaggacgct cacgcgggag gggatctcgg tcatcttcat cacgcacaaa ctcaacgagg
     2101 tgcttgagat cgccgaccgc atcacggtgc tccggcgcgg caaacgtgtc gagacgatcc
     2161 cgcgcgctgg tgcgacggaa gccggtctcg cccgctcgat ggtcgggcgc gaggtccttc
     2221 tccgtgtgga gaagaaggca gcgacgcccg gcgagccact gctccaggtc aaggacctgg
     2281 tcgtacgcga cgaccgaggc ctcgatgcgg tgcgcggcgt cacattcgac gtgcgcgccg
     2341 gtgagatcgt cgggatcgcc ggcgtcgacg gcaacggtca gtcggagctc atcgatgcgc
     2401 tgaccggcct acggcatgca gcatcgggct cgatccaggt agcaggagag gacgtgacga
     2461 gcgcgaccgc ccgcggtacg ctcgacgccg ggatcggcca cattcccgag gaccgccagc
     2521 gccgggggct ggtgctcgag ttcaacctgg cggagaatct cgtcctccac gactatggca
     2581 aggagccgtt ctcacgcttc gggttgctca atccccggag cctcttcggc tgggctcgca
     2641 agctgctgca ggagttcgac gtgcgcggcg gcggaccgga gacacgcggc gcctcgctgt
     2701 cgggcggcaa ccagcagaag gtcgtgctgg cgcgcgagat caaccgcgac ccgcgtctgc
     2761 tgatcgccgg acagccgacg cgcgggctcg acgtcggtgc gatcgaattc gtccaccagc
     2821 gcctcgtcga gcagcgagac gaaggtaaag ccgtcctgct cgtctcgctc gaactggagg
     2881 agattctgtc gttgtccgat cgcatcctcg tcctgtacga gggtcggatc gtcggcgagt
     2941 tcacgccgag cgtcagcgag gagcaactcg gcatcgccat gacgggcgga cgagtggaag
     3001 cagcatgagc gtcgcagagg ctgcggagtg agcgactccg agagggagcc gccgaacggg
     3061 gaaggtggtg caccggcgcc gctcgacgtc cagccggagg gctatgcgcc cagtgtcgcc
     3121 gcacggcttg cccggtatca gcgtgccggc gggatcatca caccctttct gacggccctc
     3181 gtcgcgttcc tgatgggcgg catcgtcgtg ctgtcgacgg gacacaaccc gatcacgacg
     3241 tacaaggcga ttttcgaagg gtccggcctc aactggttct tccatttcgg cagccatcag
     3301 atcacgatcc cgggcacaga ccaccaggtc tggttctggt gggacacgaa cacgaatcac
     3361 gtagccgcgt acaacctcac gcagacgttg atcacgacga cgccgttgat cctgacgggt
     3421 ctggccgtcg ccttcgcata tcgctgcgga ctgttcaaca tcggcggaca gggccagtac
     3481 accgtcggtg cgatcgtggg cgtcttcgtg ggctcgcggt tcgtcgagct ggcccacttc
     3541 ccgcacgttc tcctggtcat cgtgctcgcg tcgctcgcag gggcggcctg ggcggcgatc
     3601 gcgggctttc tgaaggcgac cgtcggcgca cacgaggtga tcacgacgat catgctcaac
     3661 tgggtcgcct actggatcgg gagctatctc ttcggccgcg gcggcccact gcagaaccac
     3721 cgtgatgcag ccgtgccggt ctcggacgac atcgtcgaca aggcgaggct gccggtgttc
     3781 tggggcaact cccacctgca ggggctgcac gtaggactct tcatcgcgat cgggatgctg
     3841 ttcgtgttct ggttcatcct gaaccgcacg acgaccggct acgaggtgcg cgccgtcggc
     3901 ttcaacccgg aagctgcgcg ctacggcggg atcagcgtct cgcggaattt cgtcctcgcg
     3961 atggcgatct ccggattgtt cgccgggctc gcgggagcga tcgacatcat ggggtggcag
     4021 ttccggctcg gtgtcctcga cgttcaggtg tcgaacatcg gcttcatcgg cattgccgtt
     4081 gcgctcctcg ggcggaacac ggcggtcggc gtcggcttct cggcgttgct cttcggcgcg
     4141 ctcttcacgg gcacctcgac tcgcagcctc gatccatcgg tctttccgcc tgaactcgcc
     4201 ggcaacctcg cgctgatgat ccaggcgctc atcctgctgt tcgtcggggc cgacgtcctg
     4261 atcctctatc tctggcaggc gaggcgaaag gtcggcctcg gtcgacgtgc cgcttcctcg
     4321 gctgctgtgg aggaagcatg atcacggcac cgaccgaagg ccgcgccctc ccgcttctga
     4381 caccgcggtg gatcgcgatc ggcggcatcg cgctcggcgt gttcgccttc tggctctcga
     4441 ttccgccgat cagcacgcgg gctccctggt ggccggtcct gatcgggttg tgcgcgatcg
     4501 ctgccggcgt ctgggccatt tcgcgcgggc agaggcgcgc aggctggggc gccgttgcgg
     4561 cgggcctgat cggaatcgcc ctgggcctcc tggcgacacg ggctagcacg ggtcacctcg
     4621 accaggtcgt cgtctggtcg gccttgctga cgggcatgct cgtctgggcg acgcccctca
     4681 cctttgcggc gatcggtggc atgttctgcg agcgaagcgg cgtcgtgaac atcggcctgg
     4741 aggggatgat gctctcaggc gccttcttcg gggtcctggg cgcggacaag ttcggcagtt
     4801 ggggctgggg gcttgtgacc gcgatgatcg ccggaggcgg gttcgcgctg gtgcatgcgt
     4861 ttttcgccat ccagctgcgc gccgatcaga tcgtcggcgg gacggcgatc aacttcctgg
     4921 cgttgggggt caccggctac ttcttcatca agatctacgg cgcgaacggc acccccggca
     4981 acctctcacg catccccgat ctcgaccggc ataccgcggt cgtcgggccg atctttcgcg
     5041 gaatcgacgg gattccattc gtcggcgacg tcctcggcca gctcaacctg atgatctggc
     5101 tgtcgttcct gatcctggtc gtcgcacaca tagtcctgtt caagaccccg atcggcctcc
     5161 ggctccgcgc ggtcggcgag catccgcgcg ccgcggacac cgtcggcgtg tccgtctacg
     5221 gggtgcgcta tgccgccacc gtcctgtcgg gcatgcttgc cgcgctcggc ggcgcgtacc
     5281 tcgcgatcgg ctacgtcggc tcgttcagcg agaacatgac cgccggtcgc ggtttcatcg
     5341 cgcttgcggc ggtcatcttc gggaactggc ggcccttcgg cgcgttcggg gccgccctac
     5401 tcttcggcgg ctcgaccgcg ctctcgttcc gcctggccga tgcgtggggg aactctgccg
     5461 tcctcttcca ggcacttcca tacgtcttga ccctgatcgc cgtcgccggc gtgatcggcc
     5521 ggtcggtccc gccggcagcg agtggcaggc cctacatcaa gcagtagagc ccgggcccgc
     5581 gttgcgctcc tgctcgggct gctgggactg ctgacgatgc cggcggcgat cgaagtcgcg
     5641 gcacgctcca agcggatcgg gctgcttgac gccgcctacg ccgttccggt cgccttcctg
     5701 ctcggcctcg tcgcgctcat catggcccgc cgggcccgtc gcaacctgcg ctggctccag
     5761 ctgcgcgagg gcggcaccag cgtcgcctcg gcggctgtcc tcgtcgcggc agtgaccgtc
     5821 tgcctcgcgc tggcggccgc gttatccgtt ggtttctacg gtctggtcgt gctctaccaa
     5881 cattctcggt gaagcccact ttcggcgcag ctcgcaagtc cctaagatgg ggcggccgcc
     5941 ttgttcgaga tcggtaacag cctccgtgag gcgcgcctgc gccagagcct cgactttccc
     6001 gagatcgaac agggaacgaa gattcgaggc aagtacctgc gcgccctgga agaggagcac
     6061 ttcgacgtcc tcccggccca gacgtacgtc aagggcttct tgcgctcgta tgccgaatac
     6121 ctcggcctcg acggccagct ctacgtcgac gagtacaact cccgtttcgt cgtcggagag
     6181 gaggagcatc cgcagtcccg ccctagacgc tccgccccgc cgtcgcgggg cgtgcaggtt
     6241 caatcgcgcg tcgtcctgct gacgctcctt ggcattacgg cggtgaccgc gctcgtgatc
     6301 gtggcgtgga ctcgcggaga gccgccgagc tcgacaccgg tcggcctcgg aacgtcgaag
     6361 cctccgacac acaagaccgc ggcgcctccg gtcacgcggg cgcttcaggt ccatctgctc
     6421 gtgagggcgt cgcgcggccc ctgctggctg caggtgcaca agacgtccgc gaccgggccg
     6481 atcctcttcc aggggacact cgatcaggga cagaagcagc tcttcaccgc gcggaagctc
     6541 tggatcacgc tcgatcgtcc ggagaacctc gtgaccgtat tgaacgggca cactcgccgc
     6601 ctccccgtcg gtggcgtgaa gaccctgatc gtgacgccgc gcgcaatccg cccggccgtg
     6661 tgaccacgcg gccccgcggg ttcatcgtcg tcaccggcag cgagctcgtc cgtggcgaac
     6721 ggcgcgacct gaatggaccg ttcctggcga ccgagctcct ccggcacggc gtcgagccgg
     6781 cccggatctc aatcgtcggc gaccgcgagg aagagctctc cgacgcactg gcccacgcac
     6841 tcggcgcggc cgacctctgc gtcgtgtccg gggggctagg tccgacccac gacgaccgga
     6901 cggtcgagct cgtcgcgcgc gccgccggag tcgggcttcg actcgacgaa gagctgtacg
     6961 agcagatcgg cggcgtctcc cgccgcttcg cggcccggct cgggcggccc tatgcggact
     7021 tcgaggccgg tgtccgaaaa caagccacga tccccgaagg cgcgcactcg ctcgggctcg
     7081 ccgggactgc gcccgggctc gtcctcgaga acgggaacac cgtcgtggtc gtgctgccgg
     7141 gccctccggc cgagctgcag cgcctgtggg agacggcgct cgagagcgag ccggtgcggc
     7201 gcgtcctcgc ccgcggacgg ccgccggagc tgcggcagct gcgcttcttc ggcgcgagtg
     7261 agtccaccgt cgcgaaggcg ctcgcggacg cgggtggcga cggggacggg gtggaggcca
     7321 cgatctgcgc ccgcgacttc gagatccacg tcgacttcgt ggtcgagccc ggcgcagaag
     7381 cgcgcgccga cgagctcacg cgggagctcg tcgcgccgct cgagcgctat ctcttcagtc
     7441 gcgacgagca tccggtcgag gagctggtgc tcgacctctg ccggagccgc gggctgacgc
     7501 tgggcgcggc ggagtcgtgc accggcggca tggtggccga gcgactcacc tcggtccccg
     7561 gctccagcga cgtcttc
//
LOCUS       DASVEU010000150         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_879578,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000150 DASVEU010000000
VERSION     DASVEU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879578"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VF225_05930"
     CDS             <1..909
                     /locus_tag="VF225_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144831.1"
                     /translation="EKGIVLTDDCARPDAIGSTYVNGDLAVATTDALQLFLNEAGRYK
                     LLTASEEVELAKRFEHGDKEAKDLMINSNLRLVVSIAKKYQGHGISLLDLIQEGIIGL
                     IRAVEKFDWRRGYKFSTYATWWIRQAVQRGVANKSRTIRIPVHIVEREQKISRAERTL
                     MAKLERDPTDEEIAETSKLSLKHVQEVRAAARAVTSLDKPVGAEGDTSFGDLIAGEDA
                     EPSEEVHVSLAETAVRSAVETLPEREREVVKLRYGMNGDPDPKSLEEIGRQMGLTRER
                     VRQIEARALQRLAERREVAALGETAA"
     gene            complement(950..1022)
                     /locus_tag="VF225_05935"
     tRNA            complement(950..1022)
                     /locus_tag="VF225_05935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(987..989),aa:Ala,seq:ggc)
     gene            1535..2002
                     /locus_tag="VF225_05940"
     CDS             1535..2002
                     /locus_tag="VF225_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144832.1"
                     /translation="MLARTTLVAIALLLLAIAIVSPHGSSSKGPYHADIVRTPGVLNP
                     DVTQANINSTVCVHGWTKTIRPPTSYTNALKRKQMREYGVGGSLSDYQEDHLISLELG
                     GHPTDPRNLWPEPYPRASDVDSIENDLNAQVCSGDLSLESAQLKESELKHRDG"
     gene            2045..4186
                     /locus_tag="VF225_05945"
     CDS             2045..4186
                     /locus_tag="VF225_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7144833.1"
                     /translation="MATIGRWSTRHPWRAIVAWLAFVVVAVAAMVATGTETLQNGAVG
                     ESARGYELLDANNLWPPPREIGYLHSTTLTVDDPAFRAAVSDVVKRLGSQEVQITTPL
                     QDARLVSRDRHSALVVATLTGAYADPVRNTILAAAPAHPDVTIEETGDITASDARDRS
                     INDDLQRAEILSVPVTLFVLLFAFGAAVAASVPVLLALTAVAAAFGLLGPISQAIPID
                     DSVRTVLVLIGMAVGVDYALFYVMRSREERRRGRPPHEALERTIRTSGRTVILSGTTV
                     IIAMAAMFLIGAKVFNSLAAATISVVACAVAGSVTVLPGVLELLGNRIDRGRIPFLPH
                     LRTDQTHSRFWTAVIDRVLKRPVLSCVLSAGLLIALALPALGLKVAKPGDNTLSPQSI
                     PELRALADIRREFPGGAETAKIVATVPTRGAAALRRQGRRLEQLALAHGIGHGPVLAT
                     SNDDGTAAALFLPLTGAGNNKASRRSILTLRNELIPRTIGTIPGARTAVTGDVAEDVD
                     FTRQLKHGLPYVLAFVLLLAFVLLLVAFRSIVVPLKAIILNLLSVGASYGVLVLVFQH
                     HWAEPLLGFTSNGAIISWLPLFLFVVLFGLSMDYHVFILSRVREGVDSGMKSDDAVRY
                     GITATAGVVTSAALVMFAVFSIFGSLSSLDVKQAGVGLAAAVLIDATIVRAVLLPASM
                     KLLGEWNWYLPRALERLPRAETEASPARDPV"
     gene            4215..5768
                     /locus_tag="VF225_05950"
     CDS             4215..5768
                     /locus_tag="VF225_05950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7144834.1"
                     /translation="MSTAAIERHALRTVAFAPALALAGLTAFLFARLLPDIAGKPFHE
                     DEAVAGLISARPLGDLLHTVVLDRGGAPLHFILAHFVLAIDTTPEALRWLSVVFALAT
                     VPLCYDLARRMAGRFAGLMAAALAATSQLLLIYGTFGRMYSLFAFASALAADLFVRAL
                     AQPKRGTALAAAAAALLPLAVHPFGAFLFAAEAAVALWLWRGLQLRPALPVLVVAVLA
                     LPLVLADLRLSERYAPEAGQNLESGTSPGPAMLHALGGAAGGRGAALVLFIALAAAGM
                     LVLARRRAAVAAFAALTLVVPPPALAIANAAGLGGDRLAPRHLIFMLPVWIALVATGV
                     TRLGALLPAKARAAAFAAILGVAALAPSAVSEPRTMRTGAERAVAAPATWLAKELAPR
                     DVLYPYSPVFLAALPVAKEARAYSREPVALARAVKRTGPAHAVFVSVPLREPANEDAL
                     RRAGVRFRAFPSWLILESRGPFTNGSDALESTVELLRTVVPLVSEPVARAYLEQIRGA
                     ACVALGRPC"
     gene            complement(5765..6139)
                     /gene="rsfS"
                     /locus_tag="VF225_05955"
     CDS             complement(5765..6139)
                     /gene="rsfS"
                     /locus_tag="VF225_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7144835.1"
                     /translation="MPNGAEATLTSLDQARRIAGLAQEKLAQDVVILDMRPACSYTDY
                     FVVCTGQNPRQTAAIYDEVHSGMKHDVRLLPSSVDGQREASWIVADYLDVVVHIFTPE
                     ARAYYRLEELWGDAPPVEVAAG"
     gene            complement(6145..>6680)
                     /locus_tag="VF225_05960"
     CDS             complement(6145..>6680)
                     /locus_tag="VF225_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEX7144836.1"
                     /translation="NGHLALARAAQERFDLDGLAVLVAERPGHKQVQLDAATRLKLAR
                     AAFPDYQVEPDPYERTVDLLRARSWSEPLFLIGADQFADFLTWKDPEGVLALARIGVA
                     TRPGFPEERLDAVLQQLSRPDRVELFEIEPVPISSREIRDRVGRGESIDGLVPPAVAD
                     VIESLGLYQDGQDGGYT"
BASE COUNT         1035 a         2343 c         2172 g         1130 t
ORIGIN      
        1 gagaaaggca tcgtgctcac cgacgactgc gcccgcccgg acgcgatcgg gagcacgtac
       61 gtgaacggcg acctcgccgt cgccaccacc gatgcgctcc agctctttct caacgaggcc
      121 ggccgctaca agctgctgac cgcctccgag gaggtggagc tcgccaaacg cttcgagcac
      181 ggcgacaagg aggcgaagga tctcatgatc aactcgaacc tccgcctcgt cgtctcgatc
      241 gcgaagaagt accagggcca cggcatctcg ctgctcgacc tgatccagga gggcatcatc
      301 gggctgatcc gcgcggtgga gaaattcgat tggcgccgcg gctacaagtt ttcgacctac
      361 gcgacctggt ggatccggca ggccgtgcag cgcggcgtgg cgaacaaatc gcgcacgatc
      421 cgcattccgg tccacatcgt cgagcgcgag cagaagatct cacgcgcgga acggacgctg
      481 atggccaagc tcgagcggga tccgacggac gaggagatcg cggaaacgtc gaagctctcg
      541 ctcaagcacg tgcaggaggt tcgcgccgct gcccgcgcgg tgacgagcct cgacaagccg
      601 gtcggcgccg aaggcgacac gagcttcggc gacctgatcg ccggcgagga cgccgagccg
      661 tcggaggaag tgcacgtgag cctcgccgag accgcagtcc gcagtgccgt cgagacgctg
      721 cccgagcgcg agcgcgaggt cgtcaagctg cgctacggga tgaacggcga tcccgacccc
      781 aagtcgctcg aggagatcgg gcgtcagatg ggactgacac gcgaacgggt gcgacagatc
      841 gaggcgcgcg ccttgcagcg gctggcagag cgacgcgagg tcgcggcgct cggcgagacc
      901 gccgcttagg gcggcgagcc cggccacagt cagccgcagg ctaattcagt ggagccagcc
      961 gggatcgaac cggcgacctc tgccatgcca tggcagcgct ctcccagctg agctatggcc
     1021 ccgcgaaggc caagtgtagc ggcgaactcg aagtctttcg cccagttgac gccggccttc
     1081 tgattgttcc gcgccggggc aagacggaac tggatagcgc tccgctggcc gaacgtttcg
     1141 agaggcagaa agtagcagcg atccaagtcg gcgcagtagg cggcgaacgc atcgatctcg
     1201 tcaggtgaat agagcttccg cataagcccg tcgcaatttc ttcgagtgga ataacaccgc
     1261 acgacgatga catcgccatg tcgtggagcc catttgcact gcacgcgtac gagactcgcg
     1321 cccagctcga agatcatgtc gtagcgtcct ccttccgcca ccggcgtgta cacgctgatg
     1381 ccgagcttga cggcggcgtg gacgatcgct gtctctgcga ctgctccttt ctgatcagtt
     1441 gtgagcatgt tgtccagact agcaacgcca tcggttgggc cgtgtcccgt ttggacacct
     1501 tgcgctgcaa cgacatcgtc agcacgctcg aacgatgttg gctcgcacca cgctcgtcgc
     1561 gatcgcacta ttgctactcg caatcgcgat cgtctcgccg cacggctctt cgtcgaaggg
     1621 cccgtatcac gccgacatcg tgcgcacgcc cggcgtgctg aacccggatg tcacgcaggc
     1681 gaacatcaac tccaccgtct gcgtgcacgg gtggacgaag acgatccggc cgccgacctc
     1741 gtacacgaac gcgctgaaac ggaaacagat gcgtgaatac ggcgtcggcg gttcgctgtc
     1801 cgactaccag gaggaccacc tgatcagcct cgagctcggc gggcatccga ccgatccgcg
     1861 aaatctctgg cccgagccgt atccacgcgc ttccgatgtg gactcgatcg agaacgacct
     1921 caacgcgcag gtctgctcgg gcgatctgtc actcgaatcg gctcagctga aggagtccga
     1981 gctcaagcat cgcgacggct aggcaaagcc gggggaccgt cctacactcg atggcctgag
     2041 atccgtggcc acgatcgggc gttggagcac gcgtcacccg tggcgcgcga ttgtggcctg
     2101 gctcgcgttc gtcgtggtcg ccgtcgcggc gatggtcgca accgggacgg agacgctcca
     2161 gaacggtgcg gtcggcgagt ctgcgcgcgg ttacgagctg ctggacgcga acaacctgtg
     2221 gccgccgccg cgcgagatcg gctacctgca cagcacgacc ctgaccgtcg acgatccggc
     2281 gttccgcgcc gccgtcagcg acgtcgtcaa acgcctcggc tcgcaggagg tccagatcac
     2341 gactccgttg caagacgcgc ggctcgtctc gcgcgaccgg cactcggccc tggtcgtcgc
     2401 gacgctcacc ggggcttacg ccgacccggt gcgcaacacg atcctcgccg ccgcgcccgc
     2461 ccaccctgac gtgacgatcg aggagaccgg cgacatcacc gccagcgatg ctcgcgaccg
     2521 ctcgatcaac gacgacctgc agcgcgcaga gatcctctcc gtccccgtca cgctattcgt
     2581 gctcctcttc gcattcggcg cagcggtcgc tgcaagcgtg ccggtgctgc tcgcactgac
     2641 agcggtcgcc gcggcgttcg ggctgctcgg cccgatcagc caggcgattc cgatcgacga
     2701 cagcgtgcgc acggtgctcg tgctgatcgg gatggcggtc ggcgtcgact acgcgctctt
     2761 ctatgtcatg cgctcgcgag aggaacgacg gcgtggacgg ccgccgcacg aggcgctcga
     2821 gcgaacgatc cgaacctccg gccgcacggt gatcctctcg ggcacgacgg tgatcatcgc
     2881 gatggcggcg atgttcctca tcggcgcgaa agtgttcaac agccttgccg cggcaaccat
     2941 ctccgtggtc gcgtgcgcgg tcgccggatc ggtcaccgtg ctgcccggcg tgctcgagct
     3001 gctcgggaac cgcatcgatc gcgggcggat tccgttcctg cctcacctcc ggaccgacca
     3061 gacccactcg cgcttctgga cagccgtgat cgaccgggtc ttgaagcgcc ccgtgctctc
     3121 gtgcgtgctc tcggcaggcc tgctgatagc gcttgcgctg cctgcgctcg ggttgaaggt
     3181 cgcgaagccg ggcgacaaca cactctcgcc gcagagcatt cccgagctgc gcgcgctcgc
     3241 cgacattcgc cgtgagtttc ccggcggagc agaaacggcg aagatcgtcg cgacagtgcc
     3301 cacgcgtggc gcggcggcgc tccgtcggca agggcggcgc ctcgagcagc ttgcgctcgc
     3361 gcacggcatc ggccacggcc cggtgctcgc aacctcgaat gacgacggca ccgcggcggc
     3421 tctcttcctg ccgctgacgg gcgccggcaa caacaaggcg agccgccgct cgatcctgac
     3481 tctccgtaac gagctgattc cgcggacgat cggcacgatc cccggcgcga ggacggcggt
     3541 gaccggcgat gtcgcggagg acgtcgactt tacccgccag ctcaaacacg ggctcccgta
     3601 cgtcctcgcg ttcgttctgc tgctcgcctt cgtgctccta ctggtcgcct tccggtcgat
     3661 cgtcgtgccg ctcaaggcaa tcatccttaa cctgctctcc gtcggcgcgt cgtacggcgt
     3721 gctcgtactc gtcttccagc atcactgggc cgagccgctc ctcgggttca cgtcgaacgg
     3781 cgcgatcatc tcgtggctgc cgctgttcct cttcgtcgtt ctgttcggct tgtcgatgga
     3841 ttaccacgtc ttcatcctca gccgcgtacg ggagggtgtg gactccggca tgaaaagcga
     3901 cgacgcggtg cgctacggga tcactgctac cgcgggcgtc gtgacgagcg ccgcgcttgt
     3961 gatgttcgcc gtcttctcga tcttcgggtc gctctcctca ctcgacgtga agcaggccgg
     4021 ggtcgggctc gcagcggcag tcctgatcga cgcgacaatc gtccgtgccg tgctcctgcc
     4081 tgcctcgatg aagctcctcg gtgaatggaa ctggtatcta ccgcgcgccc tggagcggct
     4141 gccgcgggcc gaaaccgagg cttcgccggc gcgcgatccg gtctagctac cgtccacccg
     4201 gggactcccg gaccatgagc accgccgcga tcgaacgaca cgcgctgcgc actgtcgctt
     4261 ttgcgcctgc gctggcactc gccggattga cggcgtttct ctttgcacgg ctcctgcctg
     4321 acatagcggg gaagcccttc cacgaggacg aggccgtcgc cggcttgatc tccgcccgac
     4381 cgctgggaga cctgctgcat acggtcgtcc tcgatcgcgg cggggcgccg ctgcacttca
     4441 tcctggccca ctttgtcctc gcgatcgaca cgacgcccga agccttgcgc tggctctcgg
     4501 tcgtgttcgc gctcgcaacg gtcccactct gttacgacct cgcgcgacgc atggccggtc
     4561 ggtttgccgg actgatggcg gctgcgctcg cagccacttc acagctgctg ctgatctacg
     4621 ggacgttcgg gcggatgtac tcgctcttcg cgttcgcgag cgcgctcgcg gccgacctct
     4681 tcgtgcgcgc gctcgcgcag ccgaagcgtg gtacggccct cgccgccgcg gccgccgcat
     4741 tgctgccgct ggccgtgcat ccgttcggcg cgttcctgtt cgcggcggag gcggccgtcg
     4801 cgctctggct ctggcgcggc ctgcagctcc ggccggcctt gccggtcctc gtggtcgctg
     4861 tgctcgcgct cccgctcgta ctcgccgacc tgcggctctc ggagcggtac gcccccgagg
     4921 caggccagaa cctcgagagc ggcacctccc cgggaccagc catgctgcac gcactcggcg
     4981 gcgcggccgg aggccgcggc gcagcgctcg tcctcttcat cgctctcgcg gcggccggga
     5041 tgctcgtgct cgcccgccgc cgggcggcgg tcgcggcctt tgcagcgctg accctcgtag
     5101 tgcccccacc cgccctcgcg atcgcgaatg cagcagggct cggcggcgac cgcctggctc
     5161 cccgccatct catcttcatg ctgccggtct ggatcgcgct cgtcgcaacc ggggtaaccc
     5221 gtctgggagc actgctgccg gcgaaggcgc gtgcggctgc gttcgccgcg atcctcggcg
     5281 ttgcagcact cgctccgagc gccgtctccg agccgcgcac gatgcggacg ggagcggagc
     5341 gagccgtcgc cgcgcccgca acctggcttg cgaaggagct cgctccacgc gacgtgctgt
     5401 atccctactc gccggtcttc cttgcggcac tccccgtcgc gaaggaagcc cgcgcgtact
     5461 cgcgcgaacc ggtcgcgctc gcccgcgccg tgaaacgcac gggcccggca cacgcggtct
     5521 tcgtgtcggt tcctctccgt gagccggcca acgaggacgc cttacggcgc gcaggcgttc
     5581 gcttccgtgc gtttccctcg tggctcatcc tcgagtcacg aggcccgttc acgaacgggt
     5641 cagacgcgct cgaatcgacg gtcgagctcc tgcggaccgt ggttccgctc gtcagcgagc
     5701 cggttgcgcg tgcctacctc gagcagatcc gcggcgcggc gtgcgtcgcg ctcgggcgac
     5761 cgtgctagcc ggccgcgacc tcgaccggtg gggcgtcgcc ccaaagctct tctagccggt
     5821 aatacgcacg cgcttccggc gtgaagatgt ggaccaccac gtcgaggtag tcggcgacaa
     5881 tccaggaggc ttcgcgctga ccgtccaccg agctcgggag gaggcgcaca tcgtgcttca
     5941 tcccgctgtg cacctcgtcg tagatggctg ccgtctgacg cgggttctgt cccgtgcaga
     6001 cgacgaagta atccgtgtag gagcaggcgg gccgcatgtc gaggatgacc acgtcctgtg
     6061 ccagcttttc ctgggccagg ccggcgatgc ggcgggcttg atcgagagac gtcagtgtgg
     6121 cttcagctcc gttcgggata ccgcctaagt gtagcccccg tcttgaccat cctggtagag
     6181 cccgagtgac tcgatcacgt cggccacagc aggaggcacg agcccgtcga tcgactcgcc
     6241 gcggccgacg cgatcccgga tctcccgcga agagatcggg accggctcga tctcgaagag
     6301 ctcgacacgg tccggtcgcg acagttgctg caagacggcg tccagacgct cctccggaaa
     6361 ccccggcctc gtcgccactc cgatccgcgc gagggcaagc accccctccg gatccttcca
     6421 cgtgaggaaa tccgcgaact ggtccgcgcc gatcaggaac aacggctcgc tccaactccg
     6481 agcgcgaagc aggtcgaccg tccgctcgta cggatcgggc tcgacctggt agtcgggaaa
     6541 ggcggcccgc gcgagcttca accgcgtggc ggcgtcgagc tggacctgct tgtggcccgg
     6601 ccgttccgcc acgagcactg cgaggccgtc gaggtcgaaa cgctcctggg ccgcgcgcgc
     6661 gagcgcgagg tgtccgttgt
//
LOCUS       DASVEU010000151         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1269383,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000151 DASVEU010000000
VERSION     DASVEU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269383"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VF225_05965"
     CDS             <1..1287
                     /locus_tag="VF225_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7144837.1"
                     /translation="RTLFQEHLEGALHRARDTGAAVAVFFVDLDNFKLINDSFGHASG
                     DELLCAVAARLKHCTRTSDTVARQGGDEFLILVPDVGAARSVPKGTSAQDSADEIAWK
                     IRKVLQEPFVVSGIEIYVTASVGISLFPLDAADPETLLKHADVAMYEAKDEGRDRHHL
                     YAGADGDALRQLSMAGRLRRAVEAGEGLVLHYQPLVNMEDKSMVGVEALIRWCDGERG
                     LVQPNDFIPLAERTGLIGPMSDWVVEQACRQAAAWRADGLDLFVSVNLPASFWQPTAM
                     RHVLGTIESFGLNADRLMIEITESAVMEERGGAMGPVLAEIHERGLKLAIDDFGTGHS
                     SLGRLSQMRVTTLKIDRSFVRGLPDDPNAAVLVTSMIQLAGSLGLYPLAEGIETEEQR
                     RFVVERGCKLGQGFLFSRPVPAEEIPALYPVLAPPA"
     gene            complement(1291..2049)
                     /locus_tag="VF225_05970"
     CDS             complement(1291..2049)
                     /locus_tag="VF225_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7144838.1"
                     /translation="MGLGTETLAAVVRTGFARTPEGARFRRRVLAVGAFGLLLLGFLA
                     AIGFARLVLLGAGTVLVLAVGAVLVLVGGAVVLPRLRRARPQIPRPRLAHIAGRSGSA
                     FGELIRVRPRPRRSVAGRRRQAVRLNARGASERRSGNIRAAVDHHHKALEIVRELGDG
                     RGEALTLNSLALAVARGGDDDAALAHFGRSLALLRELDDAQTEGQVVANIGLVHGRRG
                     RRQQSAECLRAALEKLSPETPDYRRVEEHLRRAS"
BASE COUNT          375 a          828 c          776 g          361 t
ORIGIN      
        1 cgaacgctct tccaggagca cctcgagggc gcgcttcacc gcgcgcgcga cactggggct
       61 gcagtcgcgg tgttcttcgt cgatctcgac aacttcaagc tgatcaacga cagcttcggc
      121 cacgcctcgg gcgacgagct gctctgcgcc gtcgcggcgc gcctcaagca ctgcacgcgc
      181 acctccgaca ccgtcgcgcg tcagggcgga gacgagttcc tcatcctcgt ccccgatgtc
      241 ggtgccgccc gaagcgtccc gaaagggacg agcgcgcagg actcggcaga cgagatcgcc
      301 tggaagatcc gcaaggtcct ccaggagccg ttcgtcgtct cggggatcga gatctatgtc
      361 acggccagcg tcgggatcag cctttttccg ctcgacgccg cggaccccga gacgcttctc
      421 aagcacgctg atgtcgccat gtacgaggcg aaggacgaag ggcgagaccg ccatcacctc
      481 tacgcgggag cagacggcga cgcgctccgg caactctcga tggccggccg cttgcggcgg
      541 gccgtcgagg cgggtgaagg gctcgtcctc cactaccagc cgctcgtgaa catggaagac
      601 aagagcatgg tgggcgtcga agcgctcatt cggtggtgcg acggcgagcg cggcctcgtc
      661 cagccaaacg acttcatccc cctggccgag cggacggggc tgatcggacc gatgagcgac
      721 tgggtcgtcg agcaggcttg ccggcaagcc gccgcctggc gggcggacgg gctcgatctg
      781 ttcgtctccg tcaacctgcc ggcatcgttc tggcaaccga cggccatgcg gcacgtcctc
      841 gggacgatcg agtcgttcgg gctgaacgcc gaccggctca tgatcgagat caccgaatcg
      901 gccgtcatgg aggaacgcgg tggcgccatg gggccggtgc tggccgagat ccacgagcgg
      961 ggcctcaagc tggcgatcga cgatttcggc accggccact cctcgctcgg acggctcagc
     1021 cagatgcgcg tcaccacgct gaagatcgat cgctccttcg tgaggggcct tcccgacgat
     1081 ccgaacgcag ccgtgctcgt gacgtcgatg attcagctcg ccggcagcct gggcctctac
     1141 cccctcgccg aaggaatcga gacggaggag cagcgccgct tcgtggtcga gcgtggctgc
     1201 aagctcggcc agggcttcct cttcagccga cccgtgcccg ccgaggagat ccccgctctc
     1261 taccctgttc tcgccccgcc cgcctagcgg tcagctggcc cgtcgcaagt gctcttcgac
     1321 ccggcggtag tccggcgtct cggggctgag cttctcgagc gccgcgcgga ggcattcagc
     1381 cgattgctga cggcgaccgc ggcgaccgtg aacgaggccg atgttggcga cgacctggcc
     1441 ctcggtctgc gcgtcgtcga gctcccgaag gagcgccagc gagcgtccga agtgtgcgag
     1501 cgccgcgtcg tcgtccccgc cgcgggccac ggcgagcgca aggctgttga gcgtcagcgc
     1561 ctctcctctc ccgtcgccga gctcgcggac gatctcgagc gccttgtggt ggtgatcgac
     1621 ggccgcgcga atatttccgc tccgacgctc ggatgcccct cgggcgttca gccgaacagc
     1681 ctgcctccgg cgcccggcga cgcttcggcg aggccgaggc cgaacccgaa tgagctcccc
     1741 gaaagcggag ccggaccggc ccgcgatatg ggcaaggcgg ggtcgcggga tctgtggccg
     1801 tgctcgccgg agacgcggca gcacgacggc cccaccgaca agcactagga cggcgccgac
     1861 agcgaggacg aggacggtgc cggcgcccaa gagaacgagc ctggcaaagc cgatcgcggc
     1921 caggaagccc agtaggagca aaccgaacgc gccaaccgcg agtacacggc ggcggaagcg
     1981 cgccccttcc ggcgtccgcg cgaacccagt gcgcacgact gcagcaagtg tctcagttcc
     2041 gagtcccatg ttcttcccct tacctggtcg aaccgcgctc attcgccgtg ggttgtcgcc
     2101 gtcctccctc ggtcggtctc acgctcgacc cgcgagacca gagtaacgaa tgccctccct
     2161 gtgcccgcca gagcgccaag gggtggcccc gacctcacac agccttgctc aggcacgttt
     2221 caccagtggc cgtgagcgaa ccgcttttcc tcccggccac gtcggctcga agcgacgacc
     2281 gtcgggcgct cgctgccgcg ctcgtcacgg tgacgctctg ggcttcggca ttcgtcggta
//
LOCUS       DASVEU010000152        11888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_300791,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000152 DASVEU010000000
VERSION     DASVEU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300791"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF225_05975"
     CDS             complement(<1..432)
                     /locus_tag="VF225_05975"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7144839.1"
                     /translation="MVPRLLTIAGSDSGGGAGIQADLKAFAAAGCYGTSAVVALTAQN
                     TQGVTAVHELPVDFVRAQLEAVYDDIGVDAAKTGMLFSRALIEAVADFLDGRGLPLVV
                     DPVMIASSGATLLQSEAVETLVSRLFPLATVVTPNLHEAEAL"
     regulatory      complement(565..656)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            671..2920
                     /locus_tag="VF225_05980"
     CDS             671..2920
                     /locus_tag="VF225_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7144840.1"
                     /translation="MAVSAALWDELLEGEQVAHVETIPSADAQTEPLPDGLAPRLREA
                     LPFDALYTHQREAFDAAARGEHVILATGTASGKSLGFNLPVLDTIAHEPKSRALYLYP
                     TKALAQDQARALAELHAPNVRAAIYDGDTPGERRGQIRKWANVILTNPDMLHVGVLPH
                     HDRWADVLHNLRYIIVDEAHVYRGVFGSHVGNVLRRLRRLAQVYGAEPQFLMASATIA
                     NPGGLARDLAGVKAAVIDDDAAPRAERTILLWNPELLDAELGLRASALGDASRLMADL
                     VSRGLRTICFAKSRKAAELIHKFTVERVDAEAAKRLAPYRAGYTPAQRRDIERRLVEG
                     ELLGVAATDALELGIDIGLLDCAISVGFPGTVASLRQQWGRAGRRGHGLAVLVASEDA
                     LDQYFVREPETLLQRRVEAAILDHANPRVLDGHVRSAAFEAPLDDDDRATLGDEALER
                     AALLPELKLTKSGYVWAGRDYPAGQFGLRSTTPDTFAVVEAQSGTVLGIVERERAYST
                     VHEGAVYLHLGESYLVRELDLQARTALVTPYKGDYYTQAKKDTNTAIEETLRTERRCG
                     LDLRFGRISVTERVVAYQKKSIRDQSTLETVELDLPETSFETEAIWYVPEPDQLAGLE
                     KMPRLLGTLHAAEHSMIALLPLWAMCDRWDIGGLSTNIHFQTDAPTVFIYDGHSGGVG
                     ITERGFNAFEGWVEDTARMLGGCPCSDGCPSCVQSPKCGNLNEMLDKDGALTLLRRMV
                     GAATSAKRS"
     gene            2983..4428
                     /locus_tag="VF225_05985"
     CDS             2983..4428
                     /locus_tag="VF225_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7144841.1"
                     /translation="MRPIPRDRVVQDLALLGAGAILPGVALTVFGHHSHDLGTSVHFI
                     GVGVSAVMASVAALALTVVGVRRQDGRVVLVGSAFTVMAALLAIHGLATPGVVIGDNG
                     VIALTGAATLPVGGAVLALSALPGLRRPQGIRSLLVLQVVALVGVIGLGVVGMAVPSL
                     VPAVPEPASPSAVAALIVGAFFYSLLILRALKTYLLTQRITDLAVVVGVAWLTAALPP
                     ALLLNYTDLGWWLGHGFELAGIVIVGTAVAIDLSQAVQSRPLVGDLRAAELVAKEEAF
                     LGARVHALMQRLAEKDESTEEHTRRVAMRAVQVGEELGLPPGRLRALAIGGLLHDIGK
                     LSVPTRILQKPAGLEEDEFAVIKRHPEWGIKLLRELGGFNSSAQELVHSHHERLDGKG
                     YPRGLNASSIGLDTRILTVCDVYDALVSPRVYRAAWSHEAALALLRDESGSAFDERCV
                     AALERVLGREQMQPVAPAFKIAAAGFASSAV"
     gene            complement(4485..6824)
                     /gene="ligD"
                     /locus_tag="VF225_05990"
     CDS             complement(4485..6824)
                     /gene="ligD"
                     /locus_tag="VF225_05990"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX7144842.1"
                     /translation="MATQKLSEYKRKRDPKATPEPFGSRKGKTKEPIFVVQRHNARRL
                     HYDFRLQKDGTLLSWAVPKGVPLEPGEQHLAVHVEDHPLEYATFEGEIPKGQYGAGTV
                     EIWDSGSYELLEAKKNGGLTVRLHGTKLQGTWALVPAHLSGDEKNWLIVRKRDETGAA
                     PRRSGRTYAPMLATLADEVPRGAGWEFEVKWDGYRAIATVAGSDVTLTSRNGNDLTSR
                     FQNVAKETTKAVKTPDCVLDGEVCALDESGRSSFSAMQQGKAGTPIVYYVFDVLEIEG
                     EPVIDLPLVERRKLLEGLLDRRNKTVRLSETFEDGQALLEAAKQRQLEGIMAKRLDSR
                     YLPGKRTRDWLKIKTHSEQEFVVAGYTKGTGRRASSFGALVLGYYVGGELVYAGNVGT
                     GFNSKEIEKLLDKLRPLRRDTPPFREVPKMPKVRKSDVIWVEPKLVAEVEFAEWTHDG
                     RLRAPSYQGLREDKPAEDVRREEPITDVVRKGSRELKLSNLDKVFFPVERITKGDLLE
                     YYRTVAPVLLPHLKERPFTMVRWPDGIEGKKFFQKDAPSHMPEWIPRFRTQVSTRESP
                     RTKKWVNFPVVNDELALLWMVNMGCIDMNTWYSRIDRPERPDWVLFDLDPSPDVGFKE
                     TVQVALLVKEALDAFGLVGFPKTSSAEGMHILVPVERRYTYADTRQFAEIVAGAIART
                     HRGLATTEWTKSKRRGVLIDANQNGEGKTIASAYSVRPRPGAPVSTPLRWSEVNEDLD
                     PLSFTMPVVLERVRKLGDLYAGVLTTRQRLTDALKALGG"
     gene            complement(6825..7628)
                     /locus_tag="VF225_05995"
     CDS             complement(6825..7628)
                     /locus_tag="VF225_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7144843.1"
                     /translation="MRPIWSGAISFGLVSVPVRMFPATDSKELRFHFLDKKDLTPIAY
                     EKVRRDDRERIDADDIVRGFEIEKGRYVPLDDEDLDRLDIELTHSIDILDFVDLDEID
                     PVYFRKAYYLLPAEGAEKPYRLLVRALEETGKVGIAKVVIRNKQHLAALRPYEGLLLL
                     ETMYYADEVRSPQALDGDLGTAELQNAEVEMAKSLVENLSEPFKPEKYDDTYRKELLD
                     LIQAKAEGQPLPEPAKEQDGEVVDLMAALRESVERTQKQRRAPSKREAS"
     gene            complement(7891..8823)
                     /locus_tag="VF225_06000"
     CDS             complement(7891..8823)
                     /locus_tag="VF225_06000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7144844.1"
                     /translation="MATDAELRLRDRLTRLRNLSLLGGTRLSGELEKLQRQLERIQAG
                     PTDEEIWRSVELARHQDRPYTLDYVKRLFDDWVELHGDRGRADDHALVIGLGRFGGRT
                     VALVGNQKGRDIKERTDRNFGMAYPEGYRKAMRLMELAELHRFPLLSLVDIPGAYPGV
                     AAEQHGQGGMIARSQALMARLGVPIVTCVIGEGGSGGAIATALADRVLMQENAIYTVI
                     SPEGCAAILWRDAGEAKKAAAAFKPDAVHCLELGVIDGIVPEPEGGAHVNHDEAARLL
                     GESLQEALEDLESVAPDDLKRRRRAKFRSLGVYA"
     gene            complement(8813..9658)
                     /locus_tag="VF225_06005"
     CDS             complement(8813..9658)
                     /locus_tag="VF225_06005"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7144845.1"
                     /translation="MSSRIDVSIEHRNVPEPPGKRHPNTCPQCSSHYRDDELEAALWV
                     CPHCGHHFTMRARARIASLADEGSFVEEASDLRSEDPLDFFDLRPYTERLAEAEMKTG
                     LGEAMVIGQGAIDGNACELAVMDFAFMGGSMGSVVGEKFSRACDGAAQRGVPLVSVTA
                     SGGARMQEGILSLMQLPKTVCSVEDLHDSGQPLISVMTHPTTAGVLASFASLGDVIVA
                     EPGALLAFTGPRVVQQTTREKLPEDFGLAEQNLRFGHIDAIVPRPELKAYVARLLRLF
                     SSNGD"
     gene            complement(9697..10473)
                     /locus_tag="VF225_06010"
     CDS             complement(9697..10473)
                     /locus_tag="VF225_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX7144846.1"
                     /translation="MSRLRAKCPDCKTFTAVALGPEYECHSCGRVYLAGMVRVPQAWG
                     EGGEAMAEAAWLDLPYPETAVVEAETLEEQTLALAADLPERPLVLGGCCCAHIGAVEG
                     LAARQGPLSVIWFDAHGDLNTLETSPSGNLWATPLRKLIDSGAIRARDVALVGARNLD
                     PPEEEFIRSQGVQVGEEGIAGALDETACAYVAFDADVLDPAELAVFMPEPDGLRLGEA
                     ERILGEIARSTQVLGAGLTAASFEPGNVLPLSRLTRALGL"
     gene            complement(10470..11381)
                     /locus_tag="VF225_06015"
     CDS             complement(10470..11381)
                     /locus_tag="VF225_06015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144847.1"
                     /translation="MSLSPPEGFRVRPATVEDAPAINELVIAADTAVQGWSDSTAVEL
                     VEWWRLTDLAANSWVVEDDSLAAYGVVMSHGESAETDGFVQPAKTGLGLGSWLLRRGE
                     ERVRELGYSAALTWCLAPDTDARGLFERSGYREVRRFFRMLVEHEAEPAEPEWPEGFR
                     VTTFEPGDERAFHAALDEAFAEEWNFVPQSFEVWAERRINVPDFDPTTWFIVREGDEI
                     AAVLRGDPERGGAAWVGALGVRPQWRKRGLGLALLRHAFAEFYRRGQPRVGLGVDAQN
                     PSGATRLYERAGMHITYEAVAFKKELT"
     gene            complement(11362..>11888)
                     /locus_tag="VF225_06020"
     CDS             complement(11362..>11888)
                     /locus_tag="VF225_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7144848.1"
                     /translation="YHEIEPSLQAGGERSYREVMTAAMRELGAPESEVSGLAEALPSW
                     EPFPEVRPALENARARGWKLAILSNTDRDFIEASLERIGVPFELAIVASEIGSYKPGH
                     AHWSRFFAETDAPRETHVHVAQSYFHDIAPANELGLRTIWINRYHERHAPPPTRELDD
                     LSNLPQALDELVAA"
BASE COUNT         1929 a         4101 c         3830 g         2028 t
ORIGIN      
        1 cagcgcctcg gcctcgtgga ggttcggcgt cacaaccgtg gcgagtggaa agaggcgcga
       61 cacgagcgtc tcgacggctt cggactgcag cagtgtcgcg ccggagctcg cgatcatgac
      121 cggatcgacg acgagtggaa ggccccgccc gtcgagaaag tccgcgacag cttcgatgag
      181 ggcacgggag aagagcatcc cggtctttgc agcgtccacg cctatgtcgt cgtagaccgc
      241 ctcgagctgg gcgcggacga aatccacggg cagctcgtgc actgccgtga caccttgcgt
      301 gttctgcgcg gtgagcgcca cgaccgcgct cgtcccgtag caccccgcgg ccgcaaaggc
      361 cttcaggtcg gcctggatcc cggctccgcc gccggagtcc gagccagcaa tcgtcaacaa
      421 acgcggaacc atccaactcc cttcgccggt caacacgggg gaacccgtgg ttcccccgtg
      481 ggccccctcc ttctagcccg tgacccgagc tgcgcagcga gcctccgccg ggcaaagccc
      541 gcctccggcg tcaacgcgtg gcgatgatcg agcgttcttc ctccctccgc cggtgctaac
      601 cggatcaggt tctacgggtg tgatctcagc ccgaacgggc accccgggga ccccaggcta
      661 ccatcgccaa atggctgtca gcgcggcgct ctgggacgaa ctgctcgagg gcgaacaagt
      721 cgctcacgtc gagacgatcc catccgccga cgcgcaaacc gagccccttc cggacggcct
      781 cgcgccgcgc ttgcgcgagg cgctcccctt cgatgccctc tacacgcacc agcgtgaagc
      841 cttcgacgca gctgcgcgcg gcgagcacgt gatccttgcg acaggaaccg cgtccgggaa
      901 gtcgctcggc ttcaaccttc ccgttctcga cacgatcgca cacgagccga agagccgtgc
      961 gctgtacctc tacccgacga aagcgcttgc acaggatcaa gcccgcgcgc tggcagagtt
     1021 gcacgcgccg aacgtccgcg ctgcgatcta cgacggcgac acgccaggcg agcgacgcgg
     1081 gcagattcgc aaatgggcga acgtcattct caccaaccca gacatgctcc acgtcggggt
     1141 gttgccgcat cacgaccgct gggccgacgt cctgcacaac ctgcgttaca tcatcgtcga
     1201 cgaggcgcac gtctaccgcg gtgtcttcgg ctcgcatgtc ggcaacgtcc tgcggcggct
     1261 gcggcggctc gcgcaggtgt acggcgccga accgcagttc ctcatggcct cggcgacgat
     1321 cgccaacccc ggcgggctcg ctcgtgacct tgcaggcgtc aaggcggccg tcatcgacga
     1381 cgatgcggct ccgcgcgccg agcggacgat cctgctctgg aacccggagt tgctcgacgc
     1441 cgagctcggt ctacgcgcaa gcgccctcgg tgacgcgtcg aggctgatgg cagaccttgt
     1501 ctcccgcggg ttgcgcacga tctgctttgc gaagagccgg aaggccgcgg aattgatcca
     1561 caagttcacc gtggaacgag tcgacgccga ggctgcaaag cggctcgctc cgtatcgcgc
     1621 cggctacacg cccgcgcagc ggcgggacat cgaacgtcgg ctcgtggagg gggaactact
     1681 gggcgtcgcc gcgacggacg cgctggagct cggcatcgac atcggcctgc tcgactgcgc
     1741 catttcggtc ggcttcccgg gaaccgtcgc gtcgctccgc caacagtggg gacgtgccgg
     1801 gcgtcgcggc cacggcctcg ccgtgctcgt cgcgagcgag gacgcgctcg accagtactt
     1861 cgtccgcgag ccggagaccc tgctgcagcg ccgcgtcgaa gcggcaatcc tcgaccacgc
     1921 caaccctcgc gttctcgacg gacacgtcag gtcagctgcc ttcgaggcgc cactggacga
     1981 cgacgaccgg gccacgctcg gagacgaggc gctcgagcgt gccgcgctgt tgccggagct
     2041 gaagctgacc aagagcggct acgtgtgggc cggacgcgac tacccggcgg ggcagttcgg
     2101 ccttcgtagc acgacgcccg acacgttcgc cgtcgtggaa gcccagagcg gaaccgttct
     2161 cggaatcgtg gagcgagagc gcgcgtactc gaccgtccac gaaggcgccg tctacctcca
     2221 cctcggcgag agctacctcg tgcgcgagct cgacctccag gcgcgcaccg cgctcgtgac
     2281 gccgtacaag ggcgactact acacgcaggc gaagaaggac acgaacaccg ccatcgagga
     2341 gacgctccgt acggagcggc gttgcgggct cgatctgcgg ttcggtcgaa tctccgtgac
     2401 cgagcgagtg gttgcgtacc agaagaagtc catccgcgac cagtcgacgc tggagaccgt
     2461 cgagctcgac ctgccggaga cgagcttcga gacggaggcg atctggtacg tgcccgagcc
     2521 cgaccagctc gccgggctgg agaagatgcc caggctgctg ggaacccttc acgccgcgga
     2581 gcactcgatg attgccttgc tcccgctgtg ggcgatgtgc gaccgctggg acatcggcgg
     2641 gctctcgacg aacatccact tccagacgga cgcaccgacg gtgttcatct acgacggaca
     2701 ttccggcggc gtgggcatca ccgaacgcgg cttcaacgcg ttcgaaggtt gggtcgagga
     2761 cacggcgcgg atgctcgggg gctgcccctg ctcggacggc tgcccctcgt gcgttcagag
     2821 ccccaagtgc ggcaatctga acgaaatgct cgacaaggac ggagcgctga ccctgctgcg
     2881 caggatggtc ggggcagcga cgtctgctaa acgcagctaa ctagccccaa agtcccccat
     2941 tcgagcgtct aagtggcgat ggtcacagcc gatctggctc ccatgcggcc aatccctcgc
     3001 gatcgcgtgg tccaggatct cgcactcctc ggggctggag cgatcctccc tggggtagct
     3061 cttaccgtct tcgggcacca ctcgcacgac ctcggaacga gcgtgcactt catcggcgtc
     3121 ggcgtcagcg ccgtgatggc gtctgtcgcc gccctcgcgc tcacggtggt cggcgtccgg
     3181 cgacaggatg ggcgagtcgt gctggttgga tcggctttca ccgtcatggc agcactcctc
     3241 gccatccacg ggctggccac acccggcgtc gtgatcggcg acaacggcgt gatcgctctg
     3301 accggagctg caacgttgcc cgtcggcggc gcggtcctcg cgctctccgc gctaccgggc
     3361 ctgcgtcgac cacaaggcat tcgctcgctt ctcgttctcc aggtcgtggc cctcgtcggc
     3421 gtcatcgggc tcggcgtcgt cggcatggcc gtgccaagcc tggtgccggc agtccccgag
     3481 cccgcaagtc cttcagccgt cgccgctctc atcgtcggag cgttcttcta ctccctgctc
     3541 atcctccggg ccctcaagac ctacctactg acgcagcgga tcaccgacct ggcggtcgtg
     3601 gtcggcgttg cctggctcac ggcagcgctg cctcctgcgc tcctcctgaa ctacacggat
     3661 ctcggctggt ggctcggcca cggcttcgag ctcgccggga tcgtgatcgt cggaacagcc
     3721 gtcgcgatcg acctcagcca ggccgtccag tcacgaccgc tcgtgggcga ccttcgggcc
     3781 gcggagctgg ttgcgaagga ggaagcattc ctcggcgcgc gcgtgcacgc gttgatgcaa
     3841 cgtctcgcgg agaaggacga gtcgacggaa gagcacacgc gacgtgtcgc catgcgcgcg
     3901 gtacaggtcg gcgaggagct cgggctccca ccggggcgat tgcgcgcgct tgcgatcggc
     3961 gggcttctac acgacatcgg gaagctttcc gtgccgaccc gcatcctgca aaagccggct
     4021 gggctggagg aagacgagtt cgccgtcatc aagcggcatc cggagtgggg catcaagctc
     4081 ttgcgagaac tcgggggttt caactcttca gcgcaggagc tcgtccacag ccatcacgag
     4141 cgcctcgacg gcaagggcta cccccgaggt ctgaacgcaa gctcgatcgg cctcgatacc
     4201 cgcatcctca ccgtttgcga cgtctacgac gcgctcgttt cacctcgggt ctatcgagcc
     4261 gcctggagcc acgaggctgc gctcgccctg cttcgagacg agagcggctc ggccttcgac
     4321 gaacgctgcg tcgctgcgct cgagcgcgtt ctcgggcggg aacagatgca gccggtcgcc
     4381 ccggcgttca agatcgcagc cgcgggcttt gcgagctcag ccgtctaggc gccggctcga
     4441 cggcgccgca taagaggccg tgcgggctat gcccgctcgg ccgtctaacc cccgagggct
     4501 ttcagcgcgt ccgtcaaccg ctgcctcgtc gtgagcacgc cggcgtagag gtcgccgagc
     4561 ttgcgaactc gctcgagcac gaccggcatc gtgaacgaga gcgggtcgag atcctcgttc
     4621 acctccgacc agcgcagagg cgtcgacacg ggcgccccgg gccgtggccg cactgaatac
     4681 gcggacgcga tcgtcttgcc ttcgccgttc tgattcgcgt cgatgagcac gccgcgccgt
     4741 ttcgacttcg tccactccgt cgttgccagc ccacgatgcg tgcgtgcgat cgcgcccgca
     4801 acgatctcgg cgaactggcg cgtgtcagcg taggtgtagc gccgctcgac cggcacgagg
     4861 atgtgcattc cctccgcgct cgacgtcttc gggaagccga cgagtccgaa ggcgtcgagt
     4921 gcttctttga ccagcagcgc gacctgcacg gtctccttga agccgacgtc aggggacgga
     4981 tcgaggtcga agagcaccca gtccggacgc tcgggcctgt cgatccgtga gtaccacgtg
     5041 ttcatgtcga tgcaacccat gttcaccatc cacagcagag cgagctcgtc gttcacgacc
     5101 gggaagttca cccacttctt cgtcctcggc gactcccgcg tcgacacctg ggtgcgaaac
     5161 ctcggaatcc actccggcat gtgagagggc gcgtccttct gaaagaactt cttgccctcg
     5221 atcccgtcgg gccagcgcac catcgtgaac gggcgctcct tcaggtgcgg caagagcacc
     5281 ggagcgacgg tgcggtagta ctcgagcagg tcgcctttgg tgatgcgttc gaccgggaag
     5341 aagaccttgt cgagattcga gagcttcagc tcccgtgacc ccttcctgac gacgtcggtg
     5401 atcggctcct cgcgtcgcac atcctccgca ggcttgtcct cgcgcaggcc ctggtacgac
     5461 ggcgcacgga gtcgcccgtc gtgtgtccac tccgcgaact cgacctcagc gacgagtttc
     5521 ggctcgaccc agatgacgtc gctcttccgc accttgggca tcttcgggac ctcgcggaac
     5581 ggcggcgtgt cccgccgcag cggcctcagc ttgtcgagca gcttctcgat ctctttgctg
     5641 ttgaagccgg tgcccacgtt gccggcgtac acgagctcgc ctccgacgta atagccgagg
     5701 acgagcgcgc cgaagctcga ggctcgcctg cccgtgccct tcgtgtaacc ggcgacgacg
     5761 aactcctgct cggagtgcgt cttgatcttc agccagtcac gcgtgcgctt gcccggcagg
     5821 tagcgggagt cgagccgctt ggccatgatt ccctcgagct gccgctgctt cgcagcctcg
     5881 agcaatgcct gtccgtcttc gaaggtctcc gagagccgaa cggtcttgtt gcgccggtcg
     5941 aggagccctt cgagcagctt gcgccgttcg acgagcggca ggtcgatcac cggctcgccc
     6001 tcgatctcga gcacgtcgaa gacgtagtag acgatgggcg ttcccgcctt gccctgctgc
     6061 atcgcggaga agctcgagcg gccggactcg tccaatgcgc agacttcgcc gtcgaggacg
     6121 cagtccggcg tcttcacggc cttcgtggtc tccttcgcaa cattctggaa gcgcgacgtc
     6181 aggtcgttcc cgttgcggct cgtgagcgtt acgtcgctgc cggcgaccgt cgcaatcgct
     6241 cggtagccgt cccacttgac ctcgaactcc cagcccgcac cacgcgggac ctcgtccgcc
     6301 agggtcgcga gcatcggcgc gtacgtgcgg ccgctccggc gaggggcagc cccagtctcg
     6361 tcgcgcttgc gaacgatcag ccagttcttc tcgtcaccgc tcaagtgggc cggcacgagc
     6421 gcccacgtcc cctggagctt cgtcccgtgc agacgtacgg tcagcccgcc gttcttcttc
     6481 gcctcgagca gctcatagct gccgctatcc cagatctcga ctgtgcccgc gccgtactga
     6541 cccttcggga tctccccctc gaacgtcgcg tactcgagcg gatggtcctc cacgtgtacg
     6601 gcgaggtgct gctcgcccgg ctcgagcggc acgcccttcg gcaccgccca gctgagcagc
     6661 gtcccgtcct tctgcagccg gaagtcgtag tggagccgcc gcgcgttgtg gcgctggacg
     6721 acgaagatcg gctccttcgt cttgcccttc ctcgagccga agggctcagg ggtcgccttt
     6781 ggatcccgct tgcgcttgta ctcggagagt ttctgggtgg ccacctacga agcttcgcgc
     6841 ttcgagggtg ccctacgctg cttctgcgtc cgctcgaccg attcccgcag cgccgccatc
     6901 aggtcgacga cctctccgtc ctgctccttt gccggctccg ggagcggctg accctcggcc
     6961 ttcgcctgga tcaggtcgag cagctccttg cgatacgtgt cgtcgtactt ctccggcttg
     7021 aacggctcgc tgaggttctc gacgagcgac ttcgccatct ccacctcggc gttctggagt
     7081 tcggctgtcc cgaggtcgcc gtcgagcgcc tgagggcttc gcacctcgtc ggcgtagtac
     7141 atcgtctcga ggaggagcag tccctcgtac gggcgtaacg cggcgagatg ctgcttgttg
     7201 cggatcacca ccttggcgat cccgacctta ccggtctcct cgagcgcccg gacgagcagg
     7261 cgatagggct tctccgctcc ctccgccggc aagaggtagt acgccttgcg gaagtagacc
     7321 gggtcgatct cgtcgaggtc cacgaaatcc aggatgtcga tggaatgcgt gagctcgatg
     7381 tcgagacggt cgaggtcctc gtcgtcgagc ggaacgtaac gccccttctc gatctcgaag
     7441 ccgcggacga tgtcgtcggc gtctatccgc tcacggtcgt cacgccggac cttctcgtag
     7501 gcaatcggcg tcaggtcctt cttgtcgagg aagtgaaagc gaagctcctt cgaatccgtc
     7561 gccgggaaca tccgcacggg aacgctgacg agaccgaacg agatcgcgcc gctccagatc
     7621 ggccgcatag cgagcggtta cccgaatggc ctcgtgtttc aaccaggggc ggcccggcaa
     7681 aggtattact tcaaaggcgt gttcttgttc ttccaaaggc ttctccaccc agcggggggt
     7741 cgactcttcg ggccggtttt ccggcccgaa gtcgttctcg agtcagggga taagcctgtg
     7801 gatgttgtgg agaggtggca cccgaccatg ccgcgaacct accgaaacct cagcgaggct
     7861 tctctctttc cacaggacaa acctgttgac tcaggcgtaa acgccgagcg agcggaattt
     7921 ggctcggcgc ctgcgcttca ggtcgtccgg agcaacggat tccaggtcct cgagcgcttc
     7981 ttgcagactt tcccccagga ggcgggcggc ttcgtcgtga ttcacgtggg cgccgccctc
     8041 gggctcgggg acgatcccgt cgatgacgcc gagctcgagg cagtggacgg cgtctggctt
     8101 gaaagccgca gccgccttct tcgcctcgcc ggcgtcccgc cagaggatgg ccgcgcaacc
     8161 ttcgggggaa atgacggtgt agatcgcgtt ctcctgcatc agcacgcggt cggcaagcgc
     8221 agtcgcaatc gcaccgccgg acccgccctc cccgatcacg caggtcacga tcggaactcc
     8281 cagtcgggcc atcagcgcct gtgaccgcgc gatcattccg ccctgcccgt gctgctcggc
     8341 agcaacgccc ggatatgcgc ccgggatgtc gacgagactg agcaatggaa accggtgcag
     8401 ctccgcgagc tccatcaggc gcatcgcctt gcgatagccc tccgggtaag ccatgccgaa
     8461 gttgcggtcg gttcgttcct tgatgtcgcg gcccttctgg ttgccgacga gcgcgacagt
     8521 gcggcccccg aatcggccta gcccgatgac cagcgcgtga tcgtccgcgc ggccgcggtc
     8581 gccgtgcaac tcgacccaat cgtcgaagag cctcttgacg tagtcgagcg tgtaggggcg
     8641 gtcttgatgc cgcgcgagct cgacggagcg ccagatctcc tcgtccgtcg gtccggcctg
     8701 gatgcgctcg agctggcgct gcagcttctc gagctcgccc gagagacgcg tgccgccgag
     8761 caggctgaga ttgcgcaggc gcgtgaggcg atcgcgcagt ctcagctcgg cgtcagtcgc
     8821 cattcgagga gaagagccgc aagagccgcg cgacgtacgc cttcaactcc ggccgcggaa
     8881 cgatcgcgtc gatgtggccg aagcggaggt tctgttcggc gagcccgaag tcctcgggca
     8941 gcttttcgcg cgtggtctgc tgcacgacgc ggggaccagt gaaggcgagc agggcacccg
     9001 gctcggcaac gatcacgtcg ccgagactgg cgaagctggc aagcacgccg gccgtggtgg
     9061 gatgggtcat cacgctgatc agcggctggc cggagtcgtg gagatcctcg actgagcaca
     9121 ccgtcttcgg aagctgcatc agcgacagga ttccttcctg catgcgggcg ccacccgacg
     9181 cagtgaccga gacgagcggc acgcctcgtt gcgcggcccc gtcacatgca cgcgaaaact
     9241 tctcgccgac gacgctgccc atcgagccgc ccatgaaggc gaagtccatc accgcaagct
     9301 cgcacgcatt cccatcgatc gctccctgac cgatcaccat cgcttctccg aggcccgtct
     9361 tcatctccgc ctcggcaagg cgttcggtgt acggccgcag gtcgaagaaa tcgagcgggt
     9421 cctccgagcg aaggtccgag gcttcctcga cgaagctgcc ttcgtccgcc agcgacgcaa
     9481 tgcgggcgcg ggcgcgcatg gtgaagtgat gcccgcagtg agggcacacc cagagcgccg
     9541 cctcgagctc gtcgtcccgg tagtgcgagg agcactgcgg gcacgtgttc gggtgccgct
     9601 tccccggcgg ctctgggacg ttccgatgct cgatggagac gtcgatccgg gaggacatgg
     9661 aactgacgag tttagactga cccggcgggg cgccagttac agccctagag cccgggtcag
     9721 cctggagagc ggcagcacgt tgcccggctc gaagctggcg gccgtcaaac cggctccgag
     9781 gacctgtgtg ctcctggcga tctcgccgag gattcgctcg gcctcgccga gcctgaggcc
     9841 gtccggctcg ggcatgaaga cggccagttc ggcgggatcc aggacgtccg cgtcgaaggc
     9901 gacatacgcg cacgcggtct cgtcgagtgc gccggcgatg ccttcctcgc ccacctggac
     9961 gccctgcgag cgaatgaact cctcctcggg cggatcgagg ttgcgcgcgc cgacgagcgc
    10021 cacgtcccgc gctcggatcg caccagagtc gatcagcttt ctcaacggcg tcgcccagag
    10081 gttcccgctc ggggaggtct cgagggtgtt gagatcgccg tgggcatcga accagatcac
    10141 cgaaagcggg ccctggcggg cagccagacc ttcgaccgca ccgatgtgcg cgcagcaaca
    10201 gccgccgagg acgagcgggc gttccggaag atccgccgcg agcgcgagcg tctgctcttc
    10261 aagcgtctcg gcctccacca cggcagtttc cgggtagggc aggtcgagcc acgcggcctc
    10321 ggccatcgcc tccccgccct cgccccacgc ctgcggcacc cgaaccattc ccgcgaggta
    10381 gacacgtcca cacgagtgac actcgtactc gggcccgagc gcgaccgccg tgaacgtctt
    10441 gcagtcgggg cacttggcgc gcaggcgact catgtgagct ccttcttgaa cgcaacggct
    10501 tcatacgtga tgtgcattcc tgcgcgctcg tagagacgcg ttgccccgct ggggttctgc
    10561 gcatcgactc cgagccccac tcgaggctgg ccccgccgat agaactcggc aaaggcgtgg
    10621 cgcaggagcg cgagaccgag accgcgctta cgccactgcg gacgtacgcc gagcgcaccg
    10681 acccaagcgg cgccaccgcg ctcgggatcg ccccttagca cggcggcaat ttcgtcgccc
    10741 tcccggacga tgaaccacgt ggtcggatcg aagtcgggga cgttgatgcg cctctcggcc
    10801 cacacctcga acgactgagg aacgaagttc cattcctctg cgaacgcctc gtccaatgca
    10861 gcgtgaaacg cgcgctcgtc gccaggctcg aacgtcgtca cgcgaaaacc ttccggccac
    10921 tccggctcgg ccggctcggc ctcgtgctcg acgagcatgc ggaagaagcg gcgtacttcg
    10981 cgatacccgg agcgctcgaa gagcccgcga gcgtcagtgt cgggcgcgag acaccacgtg
    11041 agcgcggcgg aataaccaag ctcgcgcacg cgctcttctc cccggcgcag tagccagcta
    11101 ccgagtccga ggccggtctt ggccggttgg acgaagccgt cggtttccgc gctctcgcca
    11161 tgagacatga caacgccgta cgcggcgagc gagtcgtcct cgacgaccca cgagttcgcg
    11221 gccaggtcgg tcagacgcca ccactccacc agttcgaccg ccgtcgagtc ggaccagccc
    11281 tggacggcgg tgtcggccgc gatcacgagc tcgttgatcg caggcgcgtc ttcgaccgtc
    11341 gccggccgga cgcgaaagcc ttcaggcggc gacaagctca tcgagagcct gggggaggtt
    11401 ggaaagatcg tcgagctcgc gcgtgggcgg cggcgcgtgc cgttcgtggt agcggttgat
    11461 ccagatcgtg cgcagtccga gctcgttcgc cggcgcgatg tcgtggaagt aactctgggc
    11521 gacgtgcacg tgcgtttcac gcggtgcgtc ggtctccgcg aagaaacgcg accaatgcgc
    11581 atggccgggc ttgtaggaac cgatctccga cgcgacgatc gcgagctcga acgggacgcc
    11641 gattcgctcc agcgacgcct cgatgaaatc acgatcggtg ttggagagga tcgcgagctt
    11701 ccagccgcgg gcgcgcgcat tctcgagcgc cggcctcacc tccgggaaag gctcccacga
    11761 gggaagcgcc tccgccaacc cggatacctc agactccgga gcgccgagct cgcgcattgc
    11821 ggcagtcatg acctctcgat agctgcgctc tcccccggcc tgaagcgacg gctcgatctc
    11881 gtggtagc
//
LOCUS       DASVEU010000153        11625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_881726,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000153 DASVEU010000000
VERSION     DASVEU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881726"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF225_06025"
     CDS             <1..241
                     /locus_tag="VF225_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144849.1"
                     /translation="AIRLAADLQGRLERLGVYEPERRPWLPHMTVVRFRERPGLRPPL
                     PALGEVSPSDAAAYHSVLRSGGAQYVVLESFALGG"
     gene            241..1338
                     /gene="recA"
                     /locus_tag="VF225_06030"
     CDS             241..1338
                     /gene="recA"
                     /locus_tag="VF225_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7144850.1"
                     /translation="MEVDREQALDAALGHIERQFGKGAVMKMNDHAHVSIGAVSTGSL
                     ALDLALGIGGLPRGRIVEIFGPESSGKTTLVYHVIAEAQRRGGICAFIDAEHSMDPTY
                     AKHIGVNIDDLLVSQPDTGEQGLEIAELLIRSGALDVVAVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLAGTLNRTDTICLFTNQLREKIGVMFGNPETTPGGRALKFYS
                     SVRLDIRRIETLKEGVEAIGNRVRVKVVKNKVAPPFKQAEFDIIYGSGISWEGTVLDA
                     GLERKAVTKSGSYFSFGDERLGQGRQNATAFLREHPDVCQAILARIQADLGPDQIASA
                     RLLPVALDAAEQNGQVKGDQKARIEKAASRK"
     gene            1338..1925
                     /locus_tag="VF225_06035"
     CDS             1338..1925
                     /locus_tag="VF225_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX7144851.1"
                     /translation="MPKVTRLAVERRGRVAVELDGAPWRTLPVDVVARAGLAEGRILD
                     RPALRLLRHELRRAEALEVAGRALRRQDLSARGITERLGRASVAPAAVEESLAVLSSA
                     GLVDDARFARTRAGNLAERGYGDAAIRYDLTRQGVDSELIQEALESLEGEGERARRLV
                     ERRGPGAKTARYLASKGFGEGAQEAAAGGDFACDP"
     gene            2186..3835
                     /gene="rny"
                     /locus_tag="VF225_06040"
     CDS             2186..3835
                     /gene="rny"
                     /locus_tag="VF225_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7144852.1"
                     /translation="MQVAIGILIGLVAGGLGGAVTVGALGKSRLGAAQQQRKQLIDDA
                     QREAETLRREAQITAREEAVKLRAEIDEEVAEHRGRIVKVEERVLAKEEEIDGKLTEM
                     GRREQGLADRETHLRQLQEEHKDAREQQFAELERISGMTVNEAKLSLLERSEDLVRHE
                     LARRVRQLEEEARTEAKRRARNLVADALQRVAASHAAETTVSVVELPSDDMKGRIIGR
                     EGRNIRSLEHLTGVDFIIDDTPQAVVLSSFDGIRREVARLTLTKLIEDGRIHPARIED
                     TYYASKAEIEDAIVQAGEQGVFEANCGEFHEELVKILGRLRYRTSYGQNVLKHTLEVV
                     HLAGIMAAEVGASVKVTKRAALLHDLGKAMTHEVEGSHASISAQLARRYGESQHVVHA
                     IEAHHYEIEPQTVEAVLLISADAISASRPGARGESLEHYIKRLESLEELAAQKPGVEK
                     VYALQAGREIRVIVKPGEVDDDAAVLLSHEIAREIEDQLEYPGQVKVTVIRESRAIDV
                     ARNVSNGGGQHESLAASVPEPASQVQPEPAPEPQVQPDQAA"
     gene            complement(3838..4461)
                     /locus_tag="VF225_06045"
     CDS             complement(3838..4461)
                     /locus_tag="VF225_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7144853.1"
                     /translation="MVFPDSTSLWLFGLAALALLAIPGPAVLYIVVQSADQGRRVGLA
                     SVAGVHLGTLVHVAAASAGLSAVIVASSVAFSAVKYAGAAYLIYLGVRKLLDRDAQTR
                     VERTREPLRRAFARGTIVNALNPKTALFFLAFLPQFVAPDRGGVWSQALVLGFVFVGL
                     GLVTDSLYALAAGTVAGLLRRKRDAVRYGSGIVFIALGATAALAKRT"
     gene            4479..5804
                     /gene="miaB"
                     /locus_tag="VF225_06050"
     CDS             4479..5804
                     /gene="miaB"
                     /locus_tag="VF225_06050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7144854.1"
                     /translation="MRRYHVTTFGCQMNAHDSERIKGMLEELGLGEAATQEEADVLVF
                     NTCTIREKPDQKFAAHLAQAAAVKRRDPGKVIAVGGCYAEAQRERLFQLYPYVDVAFG
                     PGSIPHLGEWLGAGGLGVARGSFGLDDRSFAGELPMHRERSFQAWVQISMGCNSKCAY
                     CIVPAVRGREVSRRPGDIVAEVTRLAAEGVRELTLLGQNVNSWGRDLAPAITTEFGEL
                     LRACDAVDGIERIRFTSPHPKDFRRPVIDAMAECEAVCEHAHLPLQSGSTRILKAMRR
                     TYNRERYLRLVAELREAIPDLALTTDLIVGFPGETEDDLLETISAVEEVGFDSAYTFV
                     YSPRAGTEAAAMTEQIPEEVKHDRIERLIEVVQHVAASRNARRVGRVEEVLVESPSRT
                     DPSLLRGRTRRNTTVNFSGAAVAGDLVDVRIDEATSTTLRGVQAVPVAA"
     gene            5804..6847
                     /locus_tag="VF225_06055"
     CDS             5804..6847
                     /locus_tag="VF225_06055"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7144855.1"
                     /translation="MRILVTGSSGQIGTNLGQRLQREGHDVFGIDKRLNTWTDDFRYL
                     LQDLSGHYPSFPGGIGGVEYPDVDLVVHLAAHAKVHQLVRQPHRALENAVMTFNVLEF
                     CRQQNLPIVFSSSREVYGDVHRFAAGEASEASADFAYTESTYSASKIAGEAFVYSYAR
                     CYGLRYLVFRFSNVYGRYDNDLARMVRVLPLFIHTMLRDESVTVFGQEKTLDFTYVDD
                     CIDGITQGIEALAEGRVVNETINLAYGNGNSLVHAAELIAAELGVEPKIALAPSLLGE
                     VTHYVADLTKARELLGYEPAVPLEEGIPRAVAWFKEHRVAHPEEDGPPPADALFPQDA
                     EIGWKSGSAVGSS"
     gene            6801..7745
                     /gene="miaA"
                     /locus_tag="VF225_06060"
     CDS             6801..7745
                     /gene="miaA"
                     /locus_tag="VF225_06060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7144856.1"
                     /translation="MPRSAGSPVLQSALRSVVAIFGPTASGKTAVAEAVAERLGGEVV
                     SADSMQAYQGLPILTAQPERPTRLVGIWSLSHEGSVAEYAQLAHAAIDEILAAGRTPV
                     VAGGTGLYLRAALAQLELPPAPSLEQRAWWEQLYDRLGPERAYDVLAQRDPGAATRLH
                     PNDRRRVVRALELSELGSSLRPTAERLWTSEVRQPTSIFGLDVPREVLTDRIEQRARS
                     MFHAGVVDEARAALAGPISSTAAQALGLRDVAELPHEEALETLVVRTRRYAAYQRKWM
                     RRIPALVSVNADRPVDETAHEIVEVASARQRLPAGRAG"
     gene            7687..8454
                     /gene="dapF"
                     /locus_tag="VF225_06065"
     CDS             7687..8454
                     /gene="dapF"
                     /locus_tag="VF225_06065"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7144857.1"
                     /translation="MKLSKWQALGNDYLLVEQAELTQPLTPDRVRQLCDYHYGVGSDG
                     LLEITSVDGTRAEVRIWNPDGSTAELSGNGARIVAAWLARRSGAERVALSVGSREVGA
                     VIEPDGNIALDVGRVDVGGKEMLDLGDERVEFTPVSVGNPHAVVRREPERGELLRLGP
                     RIERHSRFPERTNVQLVRVDGPHDLTAGVWERGAGETLSSGTSSVAAAAAAIANGWCE
                     SPVHVHLPGGDLLVELDTSGKARLTGPAEEICRVELD"
     gene            complement(8451..9197)
                     /locus_tag="VF225_06070"
     CDS             complement(8451..9197)
                     /locus_tag="VF225_06070"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX7144858.1"
                     /translation="MNAERVEEMIAALDRGELRVAEPDGDDWRVNPEAQEAILEYFRS
                     REMEPQEVGPFEYHDKIPLKRDYARLGVRVVPPAVARYGAFLSPGVVMMPSYVNIGAW
                     VGPRTMVDTWATVGSGAQIGADVHLAGGVGIGGVLEPPGSRPVIVEDGAFIGSRTILT
                     EGVRIGAGAVLGPNVSLTSSVPIIDVTGEHPVEHRGFVPPRAIVVPGTRPKNFPAGTY
                     HLACALIIGWRSESTDLKVSLNDALREHGL"
     gene            complement(9194..10351)
                     /locus_tag="VF225_06075"
     CDS             complement(9194..10351)
                     /locus_tag="VF225_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7144859.1"
                     /translation="MTPLSLSPALKATGTYPFVKLEEAKRRLAAEGVELIDFGKGDPR
                     EPTDPMIRQALADSLAEISTYPLAEGLPELRGAVAGWCGRRFRVELDPDTEIIPTYGS
                     KEAIFLLAQILIDRDGGRRLVVTTEPGYPVPDRGAVFAGADVMQLPLLEQNGFLPDLD
                     AVDSAAWQRAAIVWINYPNNPTGAVAPLDFLERLADLSAEHGFLLASDEAYTELWFDE
                     PPHSALEVRDRGNVAVFNTLSKRSSMTGYRSGFVAADTDLIAALKQFRPSVGTAPQEF
                     VQRASVVAWNDEEHVERTRAAYRRKREALLPTLARKGIRVAGSDATMYLWLEVPAGEA
                     SETFAGRLLEHGLIVSPGVFFGPAGEGYWRLALVPGEADCRRAAGILEQVL"
     gene            10385..>11625
                     /gene="hflX"
                     /locus_tag="VF225_06080"
     CDS             10385..>11625
                     /gene="hflX"
                     /locus_tag="VF225_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7144860.1"
                     /translation="MTQRQPDPALERGFIVAVLAQGVDSEGELAELRELADTALVEPI
                     GELVQHRPRPERRTYVGKGKLEELKRSYKESGAEVLLVDDELDPAQQRTLEDSLQARV
                     VDRTQLILDIFAQHARSAEGKLQVELAQLEYNLPRMRGMWQHLERLGGGMGSLGAGVG
                     TRGPGESQLETDRRIARRRITLLRRRLKDLSKQRDVRRKERQRSEAPTVALAGYTNVG
                     KSTLLNALTEADVSVNDRLFETLDPTTRSFDHDGRRYLVTDTVGFIRRLPTQLVQGFA
                     ATLEETLVADLILLVVDASAPDDRLDEQEAAVRAVLHDIGADDLPLEMVVNKIDAVDA
                     LRRRRLANRFPDAVQISALTGRGLDKLRARIAERFADRFEAVHLFIPYEDGGKLAELY
                     ALGAPIDEREDQEAGVHVRARL"
BASE COUNT         1985 a         3895 c         3995 g         1750 t
ORIGIN      
        1 agcgatccgg ctggcggccg acctccaggg ccgcctggag cgtctgggcg tctacgagcc
       61 ggagcgccgc ccctggctcc cgcacatgac ggtcgtccgc ttccgggagc gcccagggct
      121 gcggccgccg ctgcctgccc tcggggaggt cagtccgtcc gatgccgctg cctaccattc
      181 cgtgctgcgg tccggtggag cgcagtatgt cgtcctcgaa tcgtttgccc taggaggttg
      241 atggaagtgg atcgcgagca ggctctggac gccgccctcg ggcacatcga gcgtcagttc
      301 ggcaagggcg ccgtcatgaa gatgaacgac cacgcgcacg tctcgatcgg cgcggtgtcg
      361 accggctcgc tcgcgctcga cctcgcgctc gggatcggcg ggctcccgcg cggccgcatc
      421 gtcgagatct tcgggccgga gtcttccgga aagacgacgc tcgtctacca cgtgatcgcg
      481 gaggcacagc gccgcggcgg catctgtgca ttcatcgacg ccgagcattc gatggacccg
      541 acgtacgcga agcacatcgg cgtcaacatc gacgacctcc tcgtttcgca gcccgacacc
      601 ggtgagcagg gactcgagat cgcggagctg ctcatccgct cgggcgcact cgacgtcgtc
      661 gcggtcgact ctgtcgcggc cctgaccccg aaggccgaga tcgagggcga gatgggcgac
      721 acgcacgtcg gcctgcaggc gcgcctgatg tcgcaagcgc tgcgcaagct cgccggcacg
      781 ctgaaccgca cggacacgat ctgcctcttc acgaaccagc tgcgcgagaa gatcggcgtc
      841 atgttcggca acccggagac aacgccggga ggtcgcgcgt tgaagttcta ctcctcggtc
      901 cgcctcgaca tccgtcgcat cgagacgctg aaggaaggcg tcgaggcgat cggcaaccgc
      961 gtgcgcgtca aggtggtcaa gaacaaggtc gcaccgccgt tcaagcaagc cgagttcgac
     1021 atcatctacg gctcgggcat ctcgtgggag ggaacggtgc tcgacgccgg gctcgagcgg
     1081 aaggccgtga cgaaatcggg gtcgtacttc agcttcggcg acgagcggct cggccaggga
     1141 cggcagaatg cgaccgcgtt cctgcgcgag catccggacg tctgccaggc aatcctcgcc
     1201 cggatccagg ccgacctcgg tcccgaccag atcgcctcgg cgcgcctgct cccggtggct
     1261 ctagacgcag ccgaacagaa cggccaggtc aagggagacc agaaagcccg gatcgagaaa
     1321 gccgcttctc gcaagtagtg cccaaggtca cgcggctcgc ggtagagcgg cgcggccgcg
     1381 tagcggtcga attggacggt gcaccctggc gcacgctgcc cgtggatgtc gtggcgcgcg
     1441 ccgggctcgc ggaagggcgc attctcgatc gccctgcgct ccgcctcttg cggcacgaat
     1501 tgcgacgcgc agaggctctc gaagtcgccg gtcgagcgct gcgccggcag gatctctcgg
     1561 cgcgtggcat cacggaacgg ctcggccgcg cctcggtggc tccggctgca gtcgaagagt
     1621 cgctcgccgt tctctcgagc gccgggctcg tcgacgacgc tcgcttcgcc cggacccgcg
     1681 ccggcaacct tgccgagcgg ggctacggcg atgccgccat caggtacgac ctcacgcggc
     1741 agggggtcga ctcggagctc atccaggagg cgctcgagag cctggagggc gagggggagc
     1801 gggcccgccg actcgtggaa cgccgcgggc cgggcgcaaa gacggcgcgc tacctggctt
     1861 cgaaaggctt tggtgagggg gcgcaggaag ccgcggccgg cggggacttt gcatgcgacc
     1921 cctgaacagc gctaggatgc aggtgcatca tccgacgttt tgcctgcata ggaacgtttt
     1981 ccgaatcgtt actgcacttc cgaagatcat gtcaatctcc caccagtcag acaccgaaag
     2041 gctcttgaca gcggcgatct aggcgaccgc tctccgagcc gcggtgtcgg ctcgtttcac
     2101 cgattcggga tctcccgtgc cggccggact gccgggtggg cgccgcggta gcgaccgcgg
     2161 acaaccgacc caggagctga gccaaatgca ggtcgccatc gggattctga tcggcctcgt
     2221 cgccggggga ttgggagggg cagtcacggt gggcgcgctc ggcaagtcgc gcctcggtgc
     2281 agcccagcag caacgtaagc agctgatcga cgacgctcag cgcgaagcgg agacgcttcg
     2341 gcgcgaggcg cagatcaccg cccgcgaaga ggcggtcaag ttgcgcgcgg agatcgacga
     2401 ggaagtcgcg gagcaccgcg gccggatcgt caaggtcgag gagcgagtgc tcgcgaagga
     2461 ggaggagatc gacggaaagc tgacggagat gggccgccgc gagcaggggc tcgccgatcg
     2521 cgagacacac ctgcgccagc tccaggaaga gcacaaggac gcgcgcgagc agcagttcgc
     2581 cgagctcgaa cggatctcgg ggatgaccgt caacgaagcg aagctcagcc tcctggaacg
     2641 ctcggaggat ctcgtccggc acgagctcgc tcgacgcgtc cggcagctcg aggaagaggc
     2701 ccgcaccgag gcgaagcgtc gcgcgcgcaa tctcgttgcg gacgcgctcc agcgggtcgc
     2761 cgcgagccac gccgccgaga cgaccgtgtc cgtcgtcgag ctcccgtccg acgatatgaa
     2821 gggtcgcatc atcggccgag agggacgcaa catccgctcc ctcgagcatc tcaccggcgt
     2881 cgacttcatc atcgacgaca cgccgcaggc ggtcgtgctg tcctcgttcg acggcatccg
     2941 gcgcgaggtc gcgagactca cgctcacgaa gctgatcgaa gacggacgga ttcacccggc
     3001 gcgcatcgag gacacgtact acgcgtcgaa ggcagagatc gaggacgcga tcgtgcaggc
     3061 cggggaacag ggagtcttcg aggccaactg cggggagttc cacgaggagc tcgtcaagat
     3121 cctcggacgg ctgcgctatc gcacgagcta cggccagaac gtcctcaagc acacgctcga
     3181 ggtcgtccac ctcgcgggca tcatggcggc ggaggtcgga gccagcgtga aggtgacgaa
     3241 acgtgcagcg ctgctgcacg acctcggcaa ggcgatgacg cacgaggtcg aaggctcgca
     3301 tgcttcgatc tccgcgcagc tggcgcgcag gtacggcgag tcgcagcatg tggtgcacgc
     3361 gatcgaggcg caccactacg agatcgagcc acagacggtc gaggcggtgc tgctgatctc
     3421 agccgacgcg atctcggctt cgaggcccgg cgcgcgcggt gagagtctcg agcactacat
     3481 caagcggctc gagtcgctcg aggagctggc ggcgcagaag ccgggagtgg agaaggtcta
     3541 cgcgctgcag gccgggcgcg agatcagggt gatcgtcaag cccggcgagg tcgacgacga
     3601 cgcggcggtg ctcctctccc acgagatcgc gcgcgagatc gaagatcagc tcgagtatcc
     3661 cgggcaggtg aaggtgacgg tcatccgcga gagccgcgcc atcgacgtcg cccgcaacgt
     3721 ttcgaacggc ggcggccagc acgagtcact cgcagcgtcg gtgccggagc cagcgtcaca
     3781 ggtccagccg gagccggcgc cggagccgca ggtccagccg gatcaggcgg cgtagtccta
     3841 cgtccgtttg gccagagcag cggtcgcgcc gagcgcgatg aaaacgatgc ccgagccgta
     3901 gcggacggca tcgcgcttac ggcgcagcaa gcctgcgacc gttccggccg ccagcgcgta
     3961 gagcgaatcg gtgacgaggc cgagcccgac gaagacgaag ccgagcacga gtgcctgcga
     4021 ccagacaccg ccgcggtcgg gagccacgaa ctgtggcagg aacgcaagga agaagagcgc
     4081 cgtcttgggg ttgagagcgt tcacgatcgt gccgcgggcg aacgcccggc ggagcggctc
     4141 gcgcgtccgc tccacacgtg tctgcgcatc tcggtcgagc agcttgcgga cgccgagata
     4201 gatcagatag gcggcgccgg cgtacttgac ggcgctgaat gcgaccgagg aggcgacgat
     4261 cacagccgac agcccggccg aggctgcggc gacgtggacg agcgtgccga gatgcacgcc
     4321 ggcgaccgag gccagcccga cgcgacggcc ctggtctgcg ctctggacga cgatgtagag
     4381 gacggccggc ccgggaatcg caagcagggc gagggccgcg aggccgaaga gccacaggga
     4441 ggtcgagtcc ggaaagacca cgggtagcat tctggcccgt gcgccgatac cacgtcacga
     4501 ccttcggctg ccagatgaac gctcatgaca gcgagcgaat caaaggcatg ctcgaggagc
     4561 tcgggctggg tgaggcggcc acgcaggagg aggctgacgt cctcgtcttc aacacttgca
     4621 cgattcgcga gaagccggac cagaagttcg cggcgcacct cgcacaggct gccgccgtca
     4681 agcggcgcga tccgggcaag gtgatcgccg tcggcggctg ctacgccgaa gcgcagcgcg
     4741 agcgcctctt ccagctctac ccgtacgtcg acgtcgcgtt cggcccggga tcgattcctc
     4801 atctcggcga atggctcggc gcgggcgggc tcggtgtcgc gcgcggaagc ttcggcctcg
     4861 acgaccgcag tttcgccggc gagttgccga tgcaccgtga acgcagcttc caggcctggg
     4921 tgcagatctc gatgggctgc aactcgaagt gcgcctactg catcgtgccc gccgttcgcg
     4981 gccgtgaggt gagccggcgc cccggggaca tcgtcgcgga ggtgacacgc ctcgcggcag
     5041 aaggagtgcg cgagctcact ctgctgggcc agaacgtcaa ctcctggggc cgcgacctcg
     5101 ctccggcgat caccaccgag ttcggcgagc tgctgcgcgc ctgcgacgcg gtcgacggca
     5161 tcgagcgcat ccgcttcacc agcccgcatc ccaaagactt ccgccgcccc gtgatcgacg
     5221 caatggccga gtgcgaggcc gtctgcgagc acgcgcacct gcctctgcag tccggctcga
     5281 cccgcatcct gaaggccatg cggcgcacgt acaaccgtga gcgctaccta cggctcgtcg
     5341 cagagctgcg cgaggcgatt ccggatctcg cgctgacgac cgacctgatc gtcggttttc
     5401 ccggcgagac cgaagacgac ctcctggaga cgatctcggc cgtcgaggag gtcggcttcg
     5461 acagtgcgta cacgttcgtg tactcgcctc gcgcgggcac cgaagccgcg gcgatgacgg
     5521 agcagattcc cgaagaggtg aaacacgatc gcatcgagcg gctgatcgag gtcgtccagc
     5581 acgtggccgc ctcacgcaac gcgaggcgtg ttgggcgagt ggaggaggtc ctcgtcgaaa
     5641 gcccgagccg caccgatccg agcctgctgc gcggccgtac gcgtcgcaac accaccgtca
     5701 acttctcggg cgccgcggtg gcaggtgacc tcgtcgacgt gcgaatcgac gaggcaacct
     5761 cgacgacctt gcgcggcgtg caggccgtgc cggttgccgc ctgatgcgga tcctcgtgac
     5821 cggctcgagc ggccagatcg ggacgaacct cggccagcgc ctgcagcgcg agggacacga
     5881 tgtcttcggg atcgacaagc ggctgaacac ctggacagac gacttccgtt atctcctgca
     5941 ggatctctcc ggtcactacc cgtccttccc aggaggcatc ggcggagtgg agtacccgga
     6001 cgtcgatctc gtcgtgcatc tcgccgcgca cgcgaaggtg caccagctcg tgcgccaacc
     6061 gcaccgtgcc ctcgaaaacg cggtcatgac cttcaacgtc ctcgagttct gccgccagca
     6121 gaatcttccg atcgtctttt cgtcgagtcg ggaggtctac ggcgacgtgc accggtttgc
     6181 tgcgggggag gcgagcgagg cgagcgcaga cttcgcgtac acggagtcca cgtattccgc
     6241 gtcaaagatc gccggggagg ctttcgtcta ctcgtatgcg cgttgctacg gcctgcgcta
     6301 tctcgtcttc cgcttctcga acgtctacgg gcgctacgac aacgatctcg cccgcatggt
     6361 gcgcgtgctg ccgcttttca tccacacgat gctgcgcgac gagtcggtga cggtgttcgg
     6421 acaggagaag acactcgact tcacctacgt cgacgattgc atcgacggga tcactcaggg
     6481 catcgaggcg ctcgccgagg ggcgcgtcgt gaacgagacg atcaacctcg cctacggcaa
     6541 cggcaactcg ctcgtccatg ccgccgagct gatcgccgcc gagctaggcg ttgagccgaa
     6601 gatcgcgctt gcgccgtcgc tgctcggcga ggtcacgcac tacgtcgccg acctgacgaa
     6661 ggcgcgcgag ctgctcgggt acgagcccgc cgtgccgctc gaggaaggga tcccccgtgc
     6721 ggtggcctgg ttcaaggagc accgtgtggc gcaccccgag gaagatgggc cgcccccggc
     6781 cgacgcgctc ttcccgcagg atgccgagat cggctggaag tccggttctg cagtcggctc
     6841 ttcgtagcgt cgttgccatc ttcggcccga ccgcgtcggg caagaccgcg gttgccgagg
     6901 cggtggccga gcgtctcggc ggcgaggttg tctcggcgga ctcgatgcag gcctaccagg
     6961 gcctgcccat cctcacggct cagccagagc gtccgacgag gctggtcgga atctggtctc
     7021 tgtcacatga gggatcggtg gccgagtacg cgcagctcgc gcatgctgcg atcgacgaga
     7081 tcttggcggc agggcgcacg cccgtcgtcg ccggcgggac cggcctgtat ctccgcgctg
     7141 cgctcgcgca gctcgagctg cctcctgcac cgagcctgga gcagcgcgcc tggtgggaac
     7201 aactctatga ccgtctagga ccggagcgag cgtacgacgt cctggctcag cgggatccgg
     7261 gcgcagcgac gcggcttcat cccaatgaca gacggcgcgt cgtccgagcg ctcgagctca
     7321 gcgagctcgg ctcctcgttg cgaccgactg ccgagcggct ctggacctcg gaggtccgcc
     7381 agcccacatc gatctttggc ctcgacgtcc cccgcgaggt gttgacggac cggatcgaac
     7441 agcgtgcccg gtcgatgttc cacgcaggcg tcgtcgacga agcccgagcc gcgcttgccg
     7501 ggccgatctc gtccaccgcc gcccaggcgc tcgggctccg cgacgtcgcg gagcttccgc
     7561 acgaggaggc gctggagaca ctcgttgttc gcactcggcg ctatgccgcc tatcaacgga
     7621 agtggatgcg acgcatcccg gcccttgtta gcgtcaacgc cgatcgtccc gtggacgaga
     7681 ccgcacatga aattgtcgaa gtggcaagcg ctcggcaacg actacctgct ggtcgagcag
     7741 gctgagctaa cgcaaccgct gacgccggac cgcgtccgtc agctctgcga ctaccactac
     7801 ggcgtcggct cggacggcct cctggagatc acctcggtcg acggaacgcg agccgaggtc
     7861 cggatctgga accccgacgg ctcgaccgcg gagctctcgg gcaacggcgc gcgcatcgtt
     7921 gccgcgtggc tcgcacgcag gtcgggagcg gagcgggtgg cactctcggt cggatcccgc
     7981 gaggtcggag cggtgatcga acccgacggc aacatcgcgc tcgatgtcgg cagggtcgac
     8041 gtggggggga aggagatgct cgatcttggt gacgagcgag tcgagttcac ccccgtgtcg
     8101 gtcggcaacc ctcatgcggt cgtccgccgc gagcccgagc gcggggagtt gcttcgacta
     8161 ggcccacgca tcgagcgtca ctcgcgtttc ccggaacgca ccaacgtgca gctggtgcgt
     8221 gtcgatgggc cgcatgacct caccgccggc gtctgggaac gcggggcggg agaaacgctg
     8281 tcgtctggaa cgagctccgt cgctgccgca gcggccgcga ttgcgaacgg ctggtgcgag
     8341 agccccgtgc acgttcatct tccgggcggg gacctcctcg tcgagctcga tacgagtggc
     8401 aaggcgcgcc tcacgggacc ggccgaagag atctgccgcg tcgagctcga ctagaggccg
     8461 tgctcccgca gggcgtcgtt caagctgacc ttcaggtcgg tcgattcgct gcgccagccg
     8521 atgatcaggg cgcacgcgag gtgatacgtc ccggcaggaa agttcttcgg ccgcgttccc
     8581 ggcacgacga tcgcgcgggg cggcacgaag ccgcggtgct cgaccggatg ctcaccggtc
     8641 acgtcgatga tcgggactga tgaggtcaac gacacattcg gcccgagcac ggcgcccgcg
     8701 ccgatgcgga caccttccgt caggatcgtg cgtgacccga tgaacgcgcc gtcctcgacg
     8761 atgacgggac gcgagccggg cggttccaac actccgccga tgccgacgcc tcctgcaaga
     8821 tgcacgtcgg cgccgatctg cgctccggac ccgaccgtcg cccacgtgtc gaccatcgtc
     8881 cgcgggccga cccaggcgcc gatgttcacg tagctcggca tcatcaccac tcccggagag
     8941 aggaaggcgc cgtagcgggc gacggcggga ggcacaaccc ggacgccgag ccgagcgtag
     9001 tcccgcttga gcggaatctt gtcgtggtat tcaaacgggc ccacctcctg cggctccatc
     9061 tcccgcgagc ggaagtactc gaggatcgcc tcctgggcct cggggttcac ccgccagtcg
     9121 tcgccgtccg gctcggcgac ccgcagctca ccgcggtcga gcgcggcgat catctcctcg
     9181 acacgctcgg cgttcacagg acctgctcga ggattccggc cgcgcggcgg cagtccgcct
     9241 cgccgggcac gagcgccagt cgccagtagc cctcgccggc gggaccgaag aacactcccg
     9301 gcgagacgat cagcccgtgt tcgagcaggc ggccggcgaa cgtctccgac gcctcgccgg
     9361 ccggcacttc gagccacagg tacatcgtcg cgtcgcttcc cgccacgcgt attcctttgc
     9421 gtgcgagtgt cggaagaagc gcctcacgct tgcggcggta ggccgcacgc gtgcgttcga
     9481 cgtgctcctc gtcgttccag gcgacgaccg aggcacgttg cacgaactcc tgcggcgccg
     9541 tgccgaccga cggcctgaac tgcttgagcg ctgcgatcag atccgtatcc gcagcgacga
     9601 agcccgagcg atagcccgtc atggacgacc gcttgctcag cgtgttgaag accgcgacgt
     9661 tcccgcggtc gcgcacttcg agcgccgagt gaggcggctc gtcgaaccag agctctgtgt
     9721 acgcctcgtc cgaggcgagc aagaatccgt gctctgccga gaggtcggcg agccgttcga
     9781 ggaagtcgag tggggcgacg gctccggtcg gattgttcgg atagttgatc cacacgattg
     9841 ccgcccgctg ccacgccgcc gagtcgaccg cgtcgagatc ggggaggaag ccgttctgct
     9901 cgagcagggg cagctgcatc acgtcggctc ccgcgaagac ggcgccgcgg tcgggcacgg
     9961 gatagccggg ctccgtcgtc acaaccaggc gcctgccgcc gtcgcgatcg atcaggatct
    10021 gcgcgagcag gaagatcgct tccttcgaac cgtacgtcgg gatgatctcg gtgtcggggt
    10081 cgagctcgac ccggaagcgt cgcccgcacc agccggcaac tgctcctcgc agctcaggca
    10141 gtccctcggc gagtgggtag gtcgagatct cggcgaggct gtccgcgaga gcctgcctga
    10201 tcatcggatc ggtcggctcg cggggatcgc ccttcccgaa gtcgatcagc tcgacgccct
    10261 ctgccgcaag ccgccgcttc gcctcttcga gcttcacgaa gggatacgtc ccggtcgcct
    10321 tgagggcggg ggagagggaa agcggcgtca tggtgggatt ctgccgagtt gccataatca
    10381 agccgtgacc caacgccagc cggaccccgc gctcgagcgc gggttcatcg tcgcggtgct
    10441 ggcccaggga gtcgattccg agggggagct ggccgagcta cgtgagctag cagacacggc
    10501 cctcgtcgag ccgatcgggg agctcgtcca gcaccgtccg cgtccggagc ggcgtaccta
    10561 cgtcggcaag gggaaactcg aggagctcaa gcgctcctac aaggaatccg gcgccgaggt
    10621 gctcctggtg gacgacgagc tcgacccggc ccagcagcgc acgctcgagg actccctcca
    10681 ggcacgcgtc gtcgaccgaa cgcagctgat cctcgacatc ttcgcccagc atgcgagaag
    10741 cgccgaggga aagctccagg tcgagctggc gcaactcgag tacaacctgc cgcgtatgag
    10801 agggatgtgg cagcacctcg agcgactcgg cggcggcatg ggctctctcg gagccggcgt
    10861 cgggacgcga gggcccggcg aatcgcagct cgagaccgac cgtcgcatcg cccgccgccg
    10921 gatcacgctc ctgcgccggc gcctgaagga cctgagcaag cagcgcgacg ttcgccgtaa
    10981 ggaacgtcag cgctcggagg cgccgaccgt cgccctggcc gggtacacca acgtcggcaa
    11041 gtcgacgctg ctgaatgctt tgaccgaggc ggacgtctcc gtcaacgacc ggctgttcga
    11101 gacgctggac ccgacgacac gctcgttcga ccacgacggg cggcgctatc tcgtaacgga
    11161 cacggtcggc ttcattcgtc gcctgccgac gcagctcgtg cagggattcg cggcgacgct
    11221 ggaggaaaca ctcgtcgcgg acctcatcct cctcgtggtc gacgcttccg ctccggacga
    11281 ccgactcgac gagcaggaag cggccgtcag ggccgtgctg cacgacatcg gcgccgacga
    11341 cctgccactc gagatggtcg tgaacaagat cgacgccgtc gacgctctgc gccgccgcag
    11401 gctcgcgaat cgcttccccg acgcggtgca gatctccgcc ttgaccggca gaggcctcga
    11461 caagttgcgt gcgcggatcg cagaacgctt cgcggatcgt ttcgaagctg tccatctctt
    11521 cattccgtac gaggacggtg gcaagctcgc cgagctgtac gcattgggtg cgccgatcga
    11581 cgagcgtgag gaccaggagg ccggcgtcca cgtccgtgcg cgcct
//
LOCUS       DASVEU010000154        11805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_882907,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000154 DASVEU010000000
VERSION     DASVEU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882907"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1659
                     /locus_tag="VF225_06085"
     CDS             217..1659
                     /locus_tag="VF225_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7144861.1"
                     /translation="MVTSDRRKWLALALLSVVQFMVVLDIAIVNVALPSIQVDLGFSQ
                     QNLQWVISAYALVFGGFLLLGGRAADLLGRRRIFLAGLIVFTLASLFAGLAWSEASLI
                     TARALQGLGAAVITPAALSILSTTFVEGRERNIALGVWGAVGGFGAAAGVLLGGILTD
                     ALSWAWIFFVNVPVGVTAFILAPILLKESRDATVKSFDALGAVLVTGGLSSLVYAITQ
                     AGQDGWLAGKTVSFFAASLVMLVGFLAWEQRHPEPLMRLGILRIKTVSGANVAGFIMG
                     TATFSLFLMLTLYMQQVLGYSPMKTGVGYLAVAGTAIFTSAIAAQLVTRIGVKPVLAI
                     GMTSLTGGLVYFTQVSVGGSYLADLLPGFLLIAVGLGFSFVPISIAALAGIQPAEAGL
                     ASGLINTSQQIGGALGIAALSTIATSRTSDALAGGATRAQALVDGFHGAFLAGVILAA
                     IGIVAALTLIRRDELEQPVEEAEPAFDLAA"
     gene            complement(1706..2374)
                     /locus_tag="VF225_06090"
     CDS             complement(1706..2374)
                     /locus_tag="VF225_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144862.1"
                     /translation="MEAAVPVGRPRSVARWAALGGLVYVVLFVIGTILLFSGAPSGDS
                     APAKVIQWYSDSGHRDRIHFGWILISLSIIFLLWFIGALRRAVSAVDGEGILTGVVSL
                     GGGVYAALALASVALSDGIRTMSDDTYQHRVFPELIHAADDATWVMHATGAAALGVMI
                     IATSLAFMWGAVWPTWAGWLGVVVGILALASIAFFPQFLFLLWILVVSIWMFRRPHSP
                     SAIA"
     gene            complement(2693..3067)
                     /locus_tag="VF225_06095"
     CDS             complement(2693..3067)
                     /locus_tag="VF225_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7144863.1"
                     /translation="MTEPALVLPTITANELLNKIENRDPFVLVDALAPMVYAHSHLPG
                     AINLPPADIEPARIARRIPDPQSEIVVYCSSPDCDDSVATARRLLGLGYTNVRHYAGG
                     KNEWRDAGLPLERAGKPFVPKP"
     gene            complement(3129..3503)
                     /locus_tag="VF225_06100"
     CDS             complement(3129..3503)
                     /locus_tag="VF225_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144864.1"
                     /translation="MGQPVVHFEVIGKDGPKLQRYYSELFGWEINGDNPMNYGMVDGK
                     KNPAPDGMSIGGGIAAGPNGYDGHVTFYVAVEDIEEALVTAERLGGTRVMGPEKVMDM
                     VELGQFKDPEGHVIGVVKPLDM"
     gene            3679..4056
                     /locus_tag="VF225_06105"
     CDS             3679..4056
                     /locus_tag="VF225_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144865.1"
                     /translation="MPKASRTTASDSMQMEGYEGHYENFDGGYTVAFETYTADADPAE
                     VFKGLPDDRCQCEHWGYVIKGKVGFKTADGEETFETGDAYYMPAGHTPVLYAGTEIIE
                     FSPTKDLQQTMDVVAKNMEGAAV"
     gene            4183..4323
                     /locus_tag="VF225_06110"
     CDS             4183..4323
                     /locus_tag="VF225_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144866.1"
                     /translation="MVARWTSDNDLITIKPTRAWRGITAVRVTTFESPSWVAWKEIRI
                     LR"
     gene            4417..4605
                     /locus_tag="VF225_06115"
     CDS             4417..4605
                     /locus_tag="VF225_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144867.1"
                     /translation="MGVLGAIVGAVAGFAIGILFVEVIFANNQSWPDVVPFVLAILGA
                     LAGSRAVRGIRSRVSNPN"
     gene            complement(4648..5061)
                     /locus_tag="VF225_06120"
     CDS             complement(4648..5061)
                     /locus_tag="VF225_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144868.1"
                     /translation="MLKNLTNVNVWVHDQDEALAFYTEKLGMELRDDVTVPELGNFRW
                     LTVGLPGQDVALALMAVPGPPVFDQETHDQITALMAKGAAGGLFFSTDDVQGTYEELK
                     KSGVEFQQEPTKQPYGIDAGFRDPSGNSARMMQSS"
     gene            complement(5098..5535)
                     /locus_tag="VF225_06125"
     CDS             complement(5098..5535)
                     /locus_tag="VF225_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7144869.1"
                     /translation="MITPVPPARHLLRAKDLIDARYREQLDVPALARAARLSTAHFSR
                     EFRRSFGETPHQYLLTRRLERAAALLRNTDRTVAEICLTVGLRSVGSFTTSFGRAFGM
                     SPTAYRASYPPASARVRIPTCVLQAYARPPYSSFREDNGDRPG"
     gene            complement(5577..6635)
                     /locus_tag="VF225_06130"
     CDS             complement(5577..6635)
                     /locus_tag="VF225_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7144870.1"
                     /translation="MAQRRFQRGRELAIDLGTANTLVFVRGGGIVVFEPSVVAIDEVT
                     GRVHAVGEEARRMIGRTPATIRAIRPLRHGVIADFDVTAEMLRYFIRRAGGGGGLSYS
                     HVVLCVPSGTTDVERNAVEEATLAAGARRVSLIEEPMAAAIGAGLAVAEAEGTMIVDI
                     GGGTTEVAVLALGGIIVWESIRVGGYELDDALVRHVDASARLLIGPETAETAKIACGA
                     AWPLPETLETQVAGRDRITGLLRRTTIDSNEVRAAIAQPIETIVDAVKRTLESTPPEL
                     AADISSRGITLAGGGALLRGFERLLSSETGLPVVVADSPLECVVLGAGSSLEESELLG
                     HTAKKRPPFGRRRRRGRR"
     gene            6770..8329
                     /locus_tag="VF225_06135"
     CDS             6770..8329
                     /locus_tag="VF225_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7144871.1"
                     /translation="MRKLKTAMAAVAVACVCAGAMAGEASAAPGKIAWSKCYAQMGPF
                     ECGTVQVPLDYDSPNGATISIAVVRLPATDPAHRIGSLFLNPGGPGGSGVDYTVFAGP
                     FLYTDEVRAKFDLVGFDPRGTNRSTALRCFGTDKQWGPYFTPFAFPSTPAEEQAWMNA
                     DLYLDSSCAQRGGRIADHMATADVARDLDVLREAVGDAKLTYAGVSYGTFLGQTYANM
                     FPNNVRALIIDGVLDPIAWTTGTGDGSTVPFSTRLRSDAGAQATLNEFFRLCDAGGAN
                     CAFGPDSATRFAALGAALKSHPLPITFPDGSTGEVNYSILIAMTLGAMYDSSSWESFA
                     EGLAGLEEQAGAATLGVRFQQFWRPTNAYIPKRGFPHYNNFLESFPAVACADSDNPHS
                     YDAWSTNGAAADAAFGYFGRLWTWASSICAAWPNSDADRYMGPFTHTTANPVLVIGNL
                     FDPATRYEGAVIASNLLPNSRLLTVHGWGHTSLFLSSCADAVQTRYLVDLTLPSVGTV
                     CEQDHVPFTGP"
     gene            8680..8856
                     /locus_tag="VF225_06140"
     CDS             8680..8856
                     /locus_tag="VF225_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144872.1"
                     /translation="MSFKDLVHYLRARFAVEEGQTMAEYGVVLAVIAVAVVVALGLLS
                     TSISAAFDAVRSKL"
     gene            complement(8952..9773)
                     /locus_tag="VF225_06145"
     CDS             complement(8952..9773)
                     /locus_tag="VF225_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144873.1"
                     /translation="MTSTTAAGTLAGGMALGGPRAILAALVLAALPIGAAGCAFEGHI
                     AVQLRDGAPVVVQQSSTVELTPNRVQGTTTRAGQVIRIDCSVTGVFDVREATGSAVLI
                     QLYVVHLRTRPLPRGTPYQLDCSGPVIVQLPADAADVQATATDGSGSSAELAVQAPLN
                     AIPLTFGKRLSAERGTQLAVVSWPAVSAGSYEMELSFSLAKTRVFREKVLYAALIACG
                     RSKYFQPIRPLTARMAQVAPLTIQPTPNQTNVSVPHLFGGIRSYGEATRTLSCAR"
     gene            9785..10237
                     /locus_tag="VF225_06150"
     CDS             9785..10237
                     /locus_tag="VF225_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144874.1"
                     /translation="MTVEFFVSPHFVVPRRLETAQFVLEPLGPQHNDADYAAWSTSIE
                     HIHNTPGWEESTWPRSMTLDENRADLERHASDFENRTGFTYTVLSPEGDVIGCVYIYP
                     SKKEAVDAEVQSWVRASDADLDTLLWRAVRTWLDSDWPFESTDYAPRD"
     gene            10230..11249
                     /locus_tag="VF225_06155"
     CDS             10230..11249
                     /locus_tag="VF225_06155"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEX7144875.1"
                     /translation="MTEQRGLDSFARAPLLFGPSPVHRLEGLSEHLGGEVEIWAKRED
                     CNSGLAYGGNKTRKLEYLVAEALAQGCDTLVSIGGVQSNHTRQVAAVAAKLGLGCVLV
                     QEHWVEWPDAVYDKVGNILLSRIMGADVRLSAAGFDIGIRPSWEEALASVEERGGKPY
                     AIPAGASDHPLGGLGFARWAQEVADQERELGVFFDTVITCSVTGSTQAGMIAGFALQD
                     AERKVLGIDGSAKVEQTWNQIARIARQTAEAIGLSRELRDDEIVLLDEWHAGTYGVPD
                     AKTIDAIRLCARLEGVLTDPVYEGKSMAALIDLVSSGRIPPGSRVLYAHLGGQPALNA
                     YSAAF"
     gene            complement(11467..>11805)
                     /locus_tag="VF225_06160"
     CDS             complement(11467..>11805)
                     /locus_tag="VF225_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144876.1"
                     /translation="RDPQASKRFYTTIAPHAGLRLAHDEPGRVQLSGPDYSFSLVRDE
                     RPLTEHVHLAFPAREDATVRAFHAAALAAGYEDQGAPGERPVYHPGYYGAFVLDPDGH
                     NVEVVNHNRG"
BASE COUNT         1914 a         3810 c         4018 g         2063 t
ORIGIN      
        1 tgccaccgct cgtcgaagcc gcgctcgcgc tctatcagcg gaaaggggcg cgtgtcgagg
       61 ccgaggcgtc acgtcgccgt cttgcggagg caagggacgg atagtccagt gtgcggttaa
      121 ccgcaccgcg attgaccggc ccagtccctt ggccagcctg ttgcgccaca ggcaacctga
      181 actagaccct ttactcctca caaaggaatg gacgacatgg ttacttctga tcgacgtaaa
      241 tggctggcac tggcgcttct gagcgtcgtc cagttcatgg tcgtcctgga catcgcaatc
      301 gtgaacgtgg cgttgccttc gattcaggtc gatctcggct tctctcagca gaacctgcag
      361 tgggtgatca gcgcgtacgc gctcgtgttc ggcgggttcc tgctcctcgg gggccgcgcg
      421 gccgacctcc tcgggcgtag acggatcttc ctcgccgggc tgatcgtgtt cacgctcgcc
      481 tcgctcttcg cgggcctggc gtggtccgag gcgtcgctga tcactgcgcg tgccctccag
      541 ggcctcggcg cggccgtcat cacgcccgcg gcgctgtcca tcctctcgac gacgttcgtc
      601 gagggacgcg agcgcaacat cgcgctcggc gtctggggcg cggtcggcgg cttcggtgcc
      661 gcggccggcg tgttgctcgg cggaatcctc acggacgcgc tcagctgggc ctggatcttc
      721 ttcgtcaacg tccccgtcgg cgtgacggcg ttcatcctcg ccccgatcct gctcaaggag
      781 agccgcgatg cgaccgtcaa gtccttcgac gcgctcggcg cggtgctggt gacgggcggg
      841 ctctcgtcgc tcgtctacgc gatcacgcag gccggacagg acggctggct cgccgggaag
      901 acggtctcgt tcttcgcagc gtcactggtc atgctggtcg gctttctcgc gtgggagcaa
      961 cgacatcccg agccgctgat gcgcctcggc atcctgcgga tcaagacggt cagcggcgcg
     1021 aacgtcgccg gcttcatcat gggcacagcg acgttctcgc tgttcctgat gttgacgctg
     1081 tacatgcagc aggtgctcgg ctactcgccg atgaagaccg gcgtcggtta cctggcggtc
     1141 gcgggcacgg cgatcttcac gtcggcgatc gcggcacagc tcgtgacccg gatcggcgtg
     1201 aagccggtgc tggcgatcgg catgacgtcg ctcaccggcg gtctcgtcta cttcacgcag
     1261 gtgtcggtgg gcggctcgta cctcgccgac ctgctgccgg gcttcctgct gatcgccgtc
     1321 ggcctcggct tctcgttcgt gccgatctcg atcgcggcgc ttgccggcat ccagcccgcc
     1381 gaggcggggc tcgcctcggg gctgatcaac acgtcacagc agatcggcgg cgcacttggg
     1441 atcgccgcgc tgtcgacgat cgccacctcg cggacgagcg atgctctcgc cggcggcgcg
     1501 acccgagcgc aggcgctggt cgacggcttc cacggcgcgt tcttggccgg agtgatcctc
     1561 gcggcaatcg ggatcgtggc cgcgctgacg ttgatccgcc gcgacgagct cgagcagccg
     1621 gtggaagagg ccgagcccgc gttcgacctg gcggcctgag aaaacgtgtg gggggagcta
     1681 cggctccccc cactgggctt ttttcttacg cgatagcgct aggcgaatgt ggacggcgga
     1741 acatccagat agagacgacc aggatccaca gcaggaacag gaactgcggg aagaacgcga
     1801 tcgaggcgag cgcgaggatc ccgacgacaa cgccgagcca gccggcccaa gtcggccaga
     1861 ccgcgcccca catgaacgca agtgaagtcg cgatgatcat cacgccgagc gccgcagcgc
     1921 ccgtggcgtg catgacccac gtcgcgtcgt ccgccgcgtg gatcaattcg gggaacacgc
     1981 ggtgctggta cgtgtcgtcg ctcatcgtcc tgatgccgtc gctcagcgcg accgaagcaa
     2041 gcgcaagcgc tgcgtagaca cctcccccga ggctcaccac cccggtgagg attccctcgc
     2101 cgtcgacggc gctcaccgcc cggcgcagcg ccccgatgaa ccagagcagg aagatgatgc
     2161 tgaggctgat caggatccag ccgaagtgga tgcggtctcg gtggccgctg tctgagtacc
     2221 actggatgac ctttgccggc gccgaatcgc cggacggcgc accgctgaag agcaggatcg
     2281 tcccgatgac gaacagcacg acgtagacaa gtcctccgag agcggcccac cgtgccacgc
     2341 tgcgcggtcg gccgacaggg actgcagctt ccattgaacc tccctctgag cgcgggcgcc
     2401 gatgcccggc ggttgggcct tcggttctct ccgtgttcgg ggagtcctgc gccggcaatc
     2461 tcggctaggg gacgagatgc ccagggcgcg acgtctacag ctcacgccgc agctcgggct
     2521 gccaggggag aagcgcaccc cgagggtaac cctgggcaag gcgctctttc gacctcaggc
     2581 gcgcaggtcc tggctagatc cgttccgatt tcctcagaac ggcgtcacgg agcctccgaa
     2641 gaccttgccg agcgggcggc ggccgctcac gttcgagttc ggccgctaga ccttacggct
     2701 tgggcacgaa aggtttcccg gcgcgctcga gcggcagtcc ggcgtcgcgc cattcgttct
     2761 tgccgccggc gtaatgtcgg acgttcgtat aaccgaggcc tagcaggcgc cgagccgtcg
     2821 caacggagtc gtcgcaatct ggactggagc agtagacgac gatctcgctc tgcggatccg
     2881 gaatgcgcct cgcgattcgc gctggctcga tgtcggctgg cggcaagttg attgcgccgg
     2941 gcagatggga gtgtgcgtac accatcggcg caagtgcgtc gaccaggacg aacgggtcgc
     3001 ggttctcgat cttgttcaga agctcgttgg ccgtgatcgt cgggagaacg agtgcgggtt
     3061 ctgtcacacg tgaaggatcg caggttgcgc ggaggctcca gcagcgccgg agcctccgca
     3121 cgccgtccct acatgtcgag cggcttgacg actccgatga cgtggccctc cggatccttg
     3181 aactggccga gctcgaccat gtccatgacc ttctccgggc ccatgacccg agtgccgccg
     3241 agacgttcgg ccgtgacgag ggcctcttcg atgtcttcga ccgcgacgta gaacgtgacg
     3301 tgtccgtcgt agccgttcgg gccggctgcg atgccgcctc cgatgctcat cccgtcgggc
     3361 gccgggttct tcttcccgtc caccatcccg tagttcatcg gattgtcgcc gttgatctcc
     3421 cagccgaaga gctcggagta gtagcgctgc agcttcgggc cgtccttccc gatcacctcg
     3481 aagtgcacga ccggctgtcc catgggtgct cccttccgcg tcgatgacga atacccagag
     3541 ttggactgca cggccgggca aaactcatcg gtgtgtggcg aggccggctc tgcggtgcgg
     3601 tgatggacct cagacggctc aacttgtctc tcccgggacg gaggcatagg ctcggcgcac
     3661 acgggaagga ggtgcgagat gccaaaggca tccaggacga cagcctcgga ctccatgcag
     3721 atggagggct acgagggcca ctacgagaac ttcgacggcg gctacacggt cgcgttcgag
     3781 acatacaccg cggacgcgga tccggccgag gttttcaaag gtctgcccga cgatcgctgc
     3841 cagtgcgagc actgggggta cgtgatcaaa ggcaaggtgg gcttcaagac cgcggacggt
     3901 gaggagacgt tcgagacggg cgacgcctac tacatgccgg ccggtcacac gccggtgctt
     3961 tatgcaggga cagagatcat cgagttcagt ccgacgaagg acctccagca gacgatggac
     4021 gtcgtcgcga agaacatgga gggggcggcc gtttgacgca agagagaact gacacaccat
     4081 ttacgtcgca ctcactactt cttaaagccg aagggagatt ctgagcgcag cctgaacgac
     4141 ctacgcggcc gcgatgcgac aggccggctt cggctgctca gggtggtcgc gcgctggacc
     4201 agcgacaacg atctcatcac catcaagccg acgcgcgcct ggcgcggcat caccgccgtg
     4261 cgggtcacga cgttcgaaag cccgtcgtgg gtcgcgtgga aagagatcag gatccttcgt
     4321 tagccgtcgg ctgctggtgg gatcgattcc cctcgcacga gctgttgccg cctggtgctc
     4381 ttgaggttca atggcctagg tgcttagctc gtccccatgg gggttctcgg ggctattgtg
     4441 ggagcggtcg ccggcttcgc catcggcatt ctcttcgtcg aggtgatctt cgcgaacaat
     4501 cagagttggc cggacgtcgt gccgttcgtg ctggccatcc tcggcgcgct cgccggaagc
     4561 cgagccgttc gcggtatccg cagtagggtc tcgaacccaa attgagctga cgaccgcaga
     4621 cgagtcgcgg caagcgcttg ttggggccta actcgactgc atcatccggg cggagttgcc
     4681 ggacgggtcg cggaagccgg cgtcgatgcc gtacggctgc tttgtcggct cctgctggaa
     4741 ctcgacgccg ctcttcttga gctcttcgta ggtgccctgg acgtcgtcgg tcgagaagaa
     4801 gagcccgccg gcggcgccct tggccatcag cgccgtgatc tggtcgtgcg tctcctggtc
     4861 gaacacgggc gggccgggga cggccatcag ggcaagggcg acgtcctgac cgggcaagcc
     4921 gacggtcagc cagcgaaagt tgcccaactc gggcaccgtg acgtcgtcgc ggagctccat
     4981 tccgagcttc tccgtgtaga aggcaagggc ttcgtcctgg tcgtggaccc agacgttgac
     5041 gttggtgagg ttcttcagca tgtcgtcctc cgatgtcgtg gaacagtcgg gacgacgtta
     5101 gccggggcga tctccgttgt cttctcgaaa actgctgtat ggcggacgcg cgtatgcctg
     5161 cagcacgcag gtcggtatcc gaacacgagc ggacgccggc ggatagctcg cccggtaggc
     5221 ggtgggcgac atgccgaacg cgcgaccgaa gctcgtcgtg aacgatccga cgctgcgcag
     5281 tccgacagtg aggcagattt ccgcgaccgt ccggtcggtg ttgcgaagca gcgcggcggc
     5341 gcgctcgagt cggcgcgtca ggaggtactg gtgtggcgtt tcgccgaagg agcgccgaaa
     5401 ctcgcggctg aagtgcgcgg tcgagagccg agcggcgcgc gcgagcgccg gcacgtccag
     5461 ctgctcccgg taacgggcgt cgatgagatc cttggcgcgg aggaggtggc gggccggtgg
     5521 gacaggcgtg atcatcggcc cagtattgct gcagtggtca ggctgtatgg gaggtctcag
     5581 cgtcgcccac gtcggcggcg acggccgaag ggcggccgct tcttcgcggt atgccccagc
     5641 agctcgctct cttcgaggct gctgccggcg ccgagcacga cgcactcgag cggcgagtcc
     5701 gcgaccacga cgggcagccc ggtttccgag ctcagtagcc gctcgaatcc acgtaacaat
     5761 gcgccgccgc cggcgagcgt gatgccgcgg ctgctgatgt ccgcggcgag ctcgggaggc
     5821 gtcgattcaa gcgtccgttt cactgcgtcg acgatcgtct cgattggctg cgcgattgcg
     5881 gcacgcacct cgttcgaatc gatcgtggtc cgtcgcagta gtccggtgat gcgatcgcgg
     5941 ccggcgactt gagtctcgag ggtttcgggc agcggccacg cggcgccgca ggcgatcttc
     6001 gccgtctcgg cggtctcggg accgatgagc aggcgcgcgc tcgcgtcgac gtgccgaacg
     6061 agcgcatcgt cgagctcgta gccgccgacg cggatcgact cccacacgat gatgccgccg
     6121 agcgcgagaa ccgccacttc ggtggtgccg ccgccgatgt ccacgatcat cgttccctct
     6181 gcctcggcga cggctagtcc ggcaccgatt gccgccgcca tcggctcctc gatcaacgac
     6241 accctgcgtg cgccggcggc gagcgttgct tcctcgactg cgttccgctc gacgtcggtt
     6301 gtcccactcg gcacgcagag aacgacgtgc gaataagaga ggccgccgcc gccgccggcg
     6361 cgccggatga agtagcgcaa catctcggct gtcacgtcga agtccgcgat cacgccgtgc
     6421 cgcaatggcc ggatcgcgcg aatggtcgcc ggcgtgcgtc cgatcatgcg gcgggcctcc
     6481 tcgccgaccg cgtggactcg gccggttacc tcgtcgatcg cgacgacgga aggctcgaag
     6541 acgacaattc cgccgccgcg aacgaagacg agagtgttcg ccgtgccgag atcgatcgcg
     6601 agctcgcgac cccgctgaaa acgccgttgt gccacagacc ctcgcccaac ggtaccggca
     6661 cttcaggatg ggccaggcga tgggccattg acttccatcc tctgaaggag cagagtcgtg
     6721 ccttgccggt gggcggcgga ggacaccggg ctctcgaggg gagggacgta tgaggaagct
     6781 aaagacagct atggcggctg tcgcagtcgc gtgcgtctgc gcgggtgcta tggcgggcga
     6841 ggcatcagcc gcgccgggaa agatcgcctg gagcaagtgc tacgcgcaga tggggccttt
     6901 cgagtgcggg actgttcagg taccgcttga ttacgacagt ccgaacggag ccacgatctc
     6961 catcgcggtc gtgcgcctac cggccaccga tccggcgcac cggatcggct ccctcttcct
     7021 gaatccgggc ggtcccggtg gctccggggt cgactacacg gtgttcgcag gcccgttcct
     7081 ctatacggac gaggttcgtg cgaagttcga tctggtcggc ttcgacccgc gtgggacgaa
     7141 caggagcacc gcgttgcgct gtttcgggac cgacaagcag tgggggccgt atttcacgcc
     7201 gtttgccttc ccgagcacac ctgcggagga gcaggcctgg atgaacgcgg atctttacct
     7261 cgattccagc tgtgcccagc gtggaggccg catcgccgac cacatggcga ctgccgacgt
     7321 cgctcgcgat ctcgatgtac tccgggaggc ggtcggcgac gccaagctca cgtatgccgg
     7381 cgtgtcctac ggaacgttcc tcgggcagac gtacgcgaac atgttcccga acaatgttcg
     7441 cgcgctgatc atcgacggcg ttctcgatcc aatcgcgtgg acgacgggca cgggcgacgg
     7501 ctcgacggtc ccgttctcaa cgcgcctacg tagcgatgcg ggtgcgcagg cgacgctgaa
     7561 cgagttcttc cgcctgtgcg acgccggcgg tgcgaactgc gccttcgggc ccgacagtgc
     7621 gactcgattc gcggcactcg gcgcggcgct gaagagtcat ccgctgccga tcacgtttcc
     7681 tgacggctcg acgggagagg tcaattattc gatcctgatc gccatgacgc tcggggcgat
     7741 gtacgactcg tcgtcgtggg agtcgtttgc ggaaggtctc gctggtctgg aggagcaggc
     7801 cggcgctgcg actctgggtg tccgctttca gcagttctgg cgtccgacaa acgcctacat
     7861 ccccaagcgc gggttcccgc actacaacaa cttcctggag agcttccctg ccgtcgcctg
     7921 cgcggactcg gacaacccgc actcctacga cgcctggtcg acgaacggtg ctgccgccga
     7981 cgccgcgttc ggctacttcg gccgactctg gacgtgggcg tcctcaatct gcgccgcctg
     8041 gccgaacagc gatgccgatc gctacatggg gccgttcacg cacaccacgg cgaaccccgt
     8101 gcttgtgatc gggaacctct tcgatccggc cacgcgctac gagggggcgg tgatcgcctc
     8161 gaacctgttg ccgaactcgc ggcttctgac ggtgcacggg tggggccaca cgtcgctctt
     8221 cctctcgtcc tgcgccgacg cggtgcagac gcgctacctc gtcgacctca cgcttccgag
     8281 cgtcggaacg gtatgtgagc aggatcacgt gccgttcact gggccctgac gaggcatctt
     8341 gcggagggtc gggcgctgta gcccgacgtt ccgagccctc ccgaattacg cgcggtacgg
     8401 gcacttggtt gcggcggagc gggcgcgcga caggttcgct ggcgacctgt aatcagccgc
     8461 gggcgggcgg ttcaccctcg tgggggatac aggtcccgcc gtccgtgacg acgatgacct
     8521 cgaaagggcc gcaggaggca tagcggcgcg gtccagagag aaaggttcat cagcttgtcg
     8581 tgcggcgttg aacgacttcg agtcgagttc acatctagca agagctctcg gtgcattgcg
     8641 cgtcatggcg cgccccaacc ggcgaaaggg ggtgaatcca tgtcgttcaa ggatctggtc
     8701 cattacctcc gcgcacgctt cgccgtcgag gagggtcaga cgatggccga gtacggcgtc
     8761 gtcctggcgg tgatcgcggt cgcagttgtc gtggcgctcg gcttgctctc cacgtccatc
     8821 tccgccgcat tcgatgcggt tcgcagcaag ctgtagttag cggtctctga gccatagccc
     8881 aagcaagcca aacgaacgag tgaatcgttc gaggcgccag ggctatggct cggcggcgtg
     8941 tcgctaggcg gtcagcgagc gcatgagagc gttcgcgttg cctctccgta gctgcgaatt
     9001 ccgccgaaca gatgcgggac cgacacgttt gtctggttcg gagttggttg gatcgtgagt
     9061 ggcgcgacct gcgccatgcg ggcggtcaac ggccggatcg gttggaagta cttcgaacgt
     9121 ccacaggcga tcaaggcggc atagagcacc ttctcccgga acacgcgggt ctttgcgagg
     9181 ctgaacgaga gctccatctc atagctgccg gcggaaaccg ccggccaact gacgacggcc
     9241 agctgcgtcc cacgctccgc cgacagccgc ttgccgaacg taagcgggat cgcattaagc
     9301 ggagcctgta cggccagctc tgccgacgag ccggacccgt ccgtcgcggt ggcctgaaca
     9361 tccgcggcat cggccggcag ctgcacgatg accgggcccg agcagtcgag ttggtagggc
     9421 gttccacgcg gcaacggccg cgtgcgcagg tggacgacgt agagctgaat cagcacagcc
     9481 gatccggtgg cctccctgac atcgaagacg cccgtgacgg agcagtcgat tcgaatgacc
     9541 tgcccggcgc gtgtggtcgt tccctgcacg cgattcggag tcagctccac ggtggatgac
     9601 tgctgcacca cgactggtgc cccatcccgg agctgaaccg cgatgtggcc ctcgaaggcg
     9661 cacccggccg cgccaatggg tagggctgcg agcacgagcg cggccaggat cgctcgaggt
     9721 ccgcctagag ccatgccgcc ggccagcgta ccggcggcgg tcgtgctcgt cataggatcg
     9781 gcgcgtgact gtcgagttct tcgtgtcgcc tcacttcgtc gtgccgcgtc gactcgagac
     9841 ggcgcagttc gtgctggagc cgctcggccc gcaacacaat gatgccgact atgcggcttg
     9901 gtcgacctcg atcgagcaca tacacaacac accgggctgg gaagagagca cctggccgcg
     9961 ttcgatgacc ctggacgaga atcgcgcgga cctcgagaga cacgcctcgg atttcgaaaa
    10021 tcggaccggc ttcacgtaca cggtgctgtc gcctgagggc gacgtgatcg gctgtgtcta
    10081 catctatccg tccaagaagg aggccgtcga cgcggaggtg caatcgtggg tacgagctag
    10141 cgacgccgat ctcgacacgc tcctctggcg agccgtccgc acatggctcg attcggactg
    10201 gcctttcgag agcacggact acgcgccccg tgactgagca gcgaggtctg gacagcttcg
    10261 cgcgcgcgcc gctgctgttc ggcccgtcgc cggtgcatcg gctcgagggg ttgagcgagc
    10321 acctcggcgg cgaggtcgag atctgggcga agcgcgagga ctgcaactcc ggtctcgcgt
    10381 acggcggcaa caagacgcgg aagctcgagt acctcgtcgc ggaagcgctc gcgcagggct
    10441 gcgacacgct tgtctcgatc ggcggcgtgc agtcgaacca cacgaggcaa gtcgccgcgg
    10501 tcgccgcgaa gctcggcctc ggctgcgtcc tggtgcagga acactgggtc gagtggccgg
    10561 atgccgttta cgacaaggtc ggcaacatcc tgctctcgcg gatcatgggc gccgacgtgc
    10621 ggctcagcgc ggccggattc gacatcggca tccggccgag ctgggaagag gccctcgcgt
    10681 cggtcgagga gcgcggcggg aagccgtacg caatccccgc cggcgcgtcc gatcacccac
    10741 tgggcgggct cggcttcgca cgctgggcac aggaggtcgc agaccaggag cgggagctcg
    10801 gcgtcttctt cgacacggtc atcacgtgct cggtgaccgg ctcaacgcag gcgggcatga
    10861 tcgcggggtt cgccttgcag gatgccgagc gcaaggtgct cggcatcgac ggctccgcga
    10921 aggtcgagca gacgtggaat cagatcgccc gcatcgcgcg gcagaccgcc gaagcgatcg
    10981 gcctgagccg cgagttgcgc gacgacgaga tcgtcctgct cgacgagtgg cacgccggca
    11041 catacggagt tccggatgcg aagacgatcg acgccatccg cctgtgtgct cggctcgagg
    11101 gagtgctcac cgatcccgtg tacgaaggaa agtcgatggc ggcactgatc gacctcgtgt
    11161 cttccggacg catcccaccc ggttcacgcg tgttgtacgc gcatctcggt ggccagcctg
    11221 cgctgaacgc ctattcagcg gccttctgac acttctcacc ggcgacatgc cgaacgcgcg
    11281 gcagaagctc gtcgtgaacg acccgccgct gcgcagtctg agggcgggaa tcgaacccgc
    11341 gagacattcc cgccggtgcc ttggctactt ccgtcgccga gcttcggtca ttgacgacgc
    11401 gctgacgact gatcgcgccg cgccccattg tcgccggatg tggtgggcgg actcgtcgcc
    11461 cacggctcag ccccggttgt ggttgacgac ctcgacgttg tgcccgtccg ggtcgagcac
    11521 gaacgcgccg tagtagcccg ggtggtagac ggggcgctcg ccgggcgcgc cctggtcctc
    11581 gtagcccgcc gcgagcgcgg cggcgtggaa cgcgcgcact gtcgcgtcct cgcgcgcggg
    11641 gaacgcgagg tggacgtgct ccgtgagggg ccgctcgtcc cggacgagcg agaagctgta
    11701 gtccgggccg gagagctgca cgcggccggg ctcgtcgtgc gccaggcgga gcccggcgtg
    11761 cggcgcgatc gtcgtgtaga agcgcttgga cgcctgcgga tcgcg
//
LOCUS       DASVEU010000155        12188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1272174,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000155 DASVEU010000000
VERSION     DASVEU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272174"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VF225_06165"
     CDS             <1..1028
                     /locus_tag="VF225_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7144877.1"
                     /translation="PEDSVPGLMHVDLGGYHISDIEFTAAFDVVKGKVGVDLADAMWA
                     HPNDTIKFAEVPKTDITVQRGMTHDGLGKYISEVVTKAPGETADVVGILRDTKTDVVV
                     NYLPVGSDAATKWYAEQVLEAGCAMVNCMPVFIAREAYWQRRFEEKGLPIIGDDIKSQ
                     VGATITHRVLTSLFRERGVRLDKTMQLNVGGNSDFLNMLERDRLESKKISKTNAVTSM
                     LDYDLGAKNVHVGPSDYVPWLSDRKWAYIRMEGSAFGDVPLNLELKLEVWDSPNSAGI
                     VIDAVRLAKLALNNGIAGALEGPSSYLMKSPPKQVPDDEAYDAAEKFIRDNARTSSRV
                     EEGATTP"
     gene            1105..2652
                     /locus_tag="VF225_06170"
     CDS             1105..2652
                     /locus_tag="VF225_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144878.1"
                     /translation="MKRPLVVLLALLVVAVAATTAGAGDNDSGFKTSQPAMLTPLAPG
                     ATVTPIITVGDTVRGYRFEAIPDGISLDPNGEGTVDVYVNHETSTVPFPYTAGAGAAN
                     QNDFDNGQLSKLRLNQHSAGVLKGELTITSAENFQRFCSNFLATSKEGFERSILFTNE
                     ETSDFVNRTGQAFPPVAGAEQAGVVIANDIRSGQHKVIYGMGRLNHENDVAVAGYGHP
                     VVLSGDDTFVSNPAGSQLYSYMADSANALWNDQGDLYGFVSDNSAVDDYYDFPVGSSM
                     SVSGHFVKIDKSAASGDQNALESASDSAHVFQFVRLEDIQYDKRPGMQNVVYIADTGR
                     GSNSASGNAFASTNGRIWKLVLDPGDPTKVLSLSVYIEGDDQPVKTIGEIHQPDNVET
                     TQNGLYITEDPGSSQQFPVGSTDPNATPARLWRHDFTTGVDAPVAVVNQSADEGPTDV
                     DSSTTLGAWGSWESTGVIDASSVFGPGAFLINVQAHTLWVEQSAAPDWTNKREGGQLM
                     LLRIPGA"
     gene            2711..4444
                     /locus_tag="VF225_06175"
     CDS             2711..4444
                     /locus_tag="VF225_06175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7144879.1"
                     /translation="MGLRICLVTPFAWSQPHDVNEHVAGVARELRTLGHTVTVLASSN
                     RARDLAAGRRALLDGLESEVIALGPAVPISRRSRIGVPVGARANLALALALGRFDVVH
                     GFEPGLPSLSYLALRDAQAMTVATFLNAERLGYPPGRAQRDRLLGRIDALVATSEETA
                     EAAATRFPGHYRVVSEGLESHLFESRHKRDLIVLEWRPNERALLRGVFRALEELPDWE
                     LVLLRTKPLAGRPTIPRVLRDRVHVRTARDGAARAPILAETSIFVPALAGLQRVRLEA
                     SASGCAIASPPGVRDQLELAGAEVARLAEDPEFRARKAERARADVVGQSFADVARELD
                     ELYRSLSGRRHSAPRSDDPLADRPWVLCDLHMHTSWSPDCAVDPADLVMHGAAIGLGA
                     IAVTDHNVFGGALETKELARDHELVVIPGEEVKTDGQGEVIGLFLREEIPRGLSFADT
                     VAAIKEQDGLVYLPHPFDRMHSIPDPATLQHHLADIDIFEVYNARLLFEAYNDEALRF
                     ARKYNLTMGAGSDAHVLQGVGTGAVRMRAFEGPEEFLLSLRSAQVLRRPRSLVYLQSL
                     KWMAQAKERVR"
     gene            4464..5045
                     /locus_tag="VF225_06180"
     CDS             4464..5045
                     /locus_tag="VF225_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX7144880.1"
                     /translation="MATTDEIYEKYLQKAIVEINDLAHEVAQVGGGTRVPVVGSGHPL
                     ADVMLLKFRPQPAEVQEGVAFYGRAGQAILKSLQRLHVDPMSVYGTNCLKFADGEEAE
                     SRPFLTRELHIVQPKLIVAMGESVVEFLNAIDFPLADTVEYKPGELERFTATVQVLVV
                     PDIDASLDEAPAKTQFWNAFKPLGQWWAELPPY"
     gene            5018..5767
                     /locus_tag="VF225_06185"
     CDS             5018..5767
                     /locus_tag="VF225_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144881.1"
                     /translation="MVGRAAALLTLGTALALYYAFHDRLWNASTWWDIAFIAFVLIPA
                     CFALVWLVLPLRNAPGLLAVGLAFGVFALLFHLAGWNTPGNFCKLFGVTAVGFWFLRY
                     FETLNWVVLVALIIPWVDAYSVWRGPTKVIVNEHRNVFTNFSFAFAIPGETSAANLGL
                     PDLLFFALFLAASVQWELRPRLTWLLLTLSFGATLALAVWGELGGLPALPLLALAFLL
                     ANGDLIWTRLRRERAARSAETGTRSSGRSEP"
     gene            complement(5700..7223)
                     /locus_tag="VF225_06190"
     CDS             complement(5700..7223)
                     /locus_tag="VF225_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7144882.1"
                     /translation="MSDTWSEGFDRRELLVRGGRLAVGAGAVAALPGWITVGARSTVD
                     ARLVELAQLVQGTVVTPADAAYDSARRLYSPRYDVVRPGAVVFCETAQDVWRTVRWSR
                     RHSIRVVARSGGHSYAGYSTCKGVVVDVTRMSGVTVNHAAGTATIGAGARLIDVDQAL
                     FQQGVMIPAGSCPTVGIAGLTLGGGHGFSGRKYGLTCDNVRGLTIVTASGQALIASQT
                     QHPDLYWACRGGGAGQFGIVTSFVFDTHAASDVVTYRATWSWSDAAAVVAAWQAWAPT
                     APDELFSMCDLIATDGPGGPIVASNGQYFGSEAALPSLLQPLLNAATPQTMTIRTRSF
                     INAALDWANCSDVARCHIGVPSAEVGRSDFLAKSDYVATPLPAAGIQAMIQKIEQRQN
                     DPALGGGSILMDAYGGAINRVPKNATAFVHRDQLFSLQYVAASSTPADADWLRGFRAA
                     LAPYVSGQAYQNYIDPELEHPNISYYATNLPRLRIVKARYDQKNVFRFPRSVLPAPA"
     gene            complement(7327..8697)
                     /gene="rlmD"
                     /locus_tag="VF225_06195"
     CDS             complement(7327..8697)
                     /gene="rlmD"
                     /locus_tag="VF225_06195"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX7144883.1"
                     /translation="MAAPVTKDQELELTIDRLAFGGNGVARLNGFVVFVRRGLPGDTV
                     RARVTKVQRRHAEALVTEVLQPSPLRVEAPCKHYPSCGGCRFQDLAYEAQVEAKHAQV
                     GDALRRIGGFAEPPLEEILPAEEIFHYRNKLEYSFTQLEDGPTLGLHRAGRWDEVLQI
                     DDCWLTTDVGNAIRNAVREWARGDKLVAYDQAEHTGYLRHLVVREGRNTGQVLVQLVT
                     SPGEKFDTGGFVDTLRQFPEVRSIHWSINDTPAEVTTNLPTQLLWGEEAIEEQLCGMR
                     FRVRPNAFLQTNTLMAEKLYETAREFAALTGDETVYDLYCGIGTIGLLLARDALTVWG
                     IDVSEESIACALENAELNGIGNAAYFAGNVGQALEELHERAGDPDVVVVDPPRAGLAG
                     KALKRLGEIGAPRIVYVSCNPTTLAGDVKRLRAEYGYELKRVKPVDMFPHTPHVEAVA
                     LLERHT"
     gene            8793..9182
                     /locus_tag="VF225_06200"
     CDS             8793..9182
                     /locus_tag="VF225_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7144884.1"
                     /translation="MDAEAAARAWVDAWDRAWRAKDESALEPVYAEDAVHRSHPDREP
                     HSPIEYARSAFADEGDDLELSWGEPLVAGNRAAVEWWAALTESGELVTLAGTSWLAFG
                     NNGRVVEQHDYWTVTPGRSAPWKGWGS"
     gene            complement(9179..9580)
                     /locus_tag="VF225_06205"
     CDS             complement(9179..9580)
                     /locus_tag="VF225_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7144885.1"
                     /translation="MEEIRLQTERRTQLVDITEQVQEALGSINGAAAALVYVPHTTAG
                     VTINEGADPAVAQDLEAALEKVVDDAWDWKHVEDPDGPNAPSHIRASLMSPQILIPLD
                     GGKLALGKWQGIFFCEFDGPRERKVYVTPLS"
     gene            9651..11147
                     /locus_tag="VF225_06210"
     CDS             9651..11147
                     /locus_tag="VF225_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144886.1"
                     /translation="MLPAPPSDRELDAYRQQADRFIAELDEEFYLHYAGLKETFDLAP
                     IYERHKNLTELDQVKSIGLAVNGGARVRELWRFACEGYFGELTRDHAEKLAELESELA
                     VSVDGEEIPYRMIRPALANEPDREKRRVLDERANELLDEHMNPLHLDAAQTVQRGTLN
                     LEFANYLELYREALPASALDDLAAQCRTFLDSTERLYEEWADKLFRTRVGVSLSEAER
                     WDVPRLFRAPEWDPMFPADKMVPALEGTLEDLGIDLSSQENVTLDLEERPNKSPRAFC
                     APIEVPDRVVLVIQPIGGPDDWRALFHEGGHTEHFAHTSRDLSMEEKRLGDNAVTEGW
                     AMLLQHLTDEPEWLTRRLDFPRPNEYAVEGATGLLYFVRRYAAKILYELEFHAAEDVT
                     TMRGRYAELLSDALKIEVTPTNYLADIDSGFYVSSYLRSWAFEAQLRAYLKEKFGSKW
                     FATREAGSLLRELWGEGQRMRAEEMLKDVTGSTLEMEAVAERVKEVLR"
     gene            11179..>12188
                     /locus_tag="VF225_06215"
     CDS             11179..>12188
                     /locus_tag="VF225_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144887.1"
                     /translation="MARTRVLIAGAAGRDFHNFNLVYRDSPAHDVVAFTATQIPNIDG
                     RRYPAELAGSLYPEGIPILPESALEQLVREHEIDVVVFAYSDVTHEHVMHIGSRALAA
                     GASFELIAPRETMLASSKPTVAICAVRTGSGKSQTTRRVAELLREAGKRVAVLRHPMP
                     YGDLTKQAVQRFERYEDLEAADATIEEREEYEPHLAEGNLVFAGVDYERILRAAEQEA
                     DVILWDGGNNDTPFIKPDLHIVVVDPHRPGHELRYHPGETNLRMADVCIVNKMDSASQ
                     EGIDAVLSSIHENNADARIVLAASPFHVEGDAEEIRGKRVLAVEDGPTLTHGEMTYGA
                     AVL"
BASE COUNT         2083 a         4094 c         4004 g         2007 t
ORIGIN      
        1 agccggagga ctccgtcccc ggcctcatgc acgtcgacct cggcggctac cacatctcgg
       61 acatcgagtt caccgccgcg ttcgacgtcg tcaagggcaa ggtcggcgtc gacctcgccg
      121 acgcgatgtg ggcgcacccg aacgacacga tcaaattcgc agaggtgccc aagacagaca
      181 tcaccgtcca acgcgggatg acccacgacg ggctcggcaa gtacatctcc gaggtcgtca
      241 ccaaggcgcc cggtgagacc gccgacgtcg tcgggatcct gcgcgacacg aagactgacg
      301 tcgtcgtcaa ctacctgccc gtcggctccg acgccgccac caagtggtat gcggagcagg
      361 tgctcgaggc tggctgcgcg atggtcaact gcatgccggt cttcatcgcc cgcgaggcgt
      421 actggcagcg ccgcttcgag gagaagggcc tgccgatcat cggcgacgac atcaagtcgc
      481 aggtcggcgc gacgatcacg caccgcgtgc tcacatcgct gttccgcgag cgcggcgtcc
      541 ggctcgacaa gacgatgcag ctgaatgtcg gcggcaactc cgacttcctc aacatgctgg
      601 aacgcgaccg gctcgagtcg aagaagatct cgaagacgaa cgccgtcacg tcgatgctcg
      661 actacgacct cggcgcgaag aacgtccacg tcggcccgtc cgactacgtg ccgtggctga
      721 gcgaccgcaa gtgggcctac atccgcatgg agggctcggc cttcggcgac gtgccgctga
      781 acctggagct gaagctcgag gtctgggact cgccgaactc ggccggcatc gtcatcgacg
      841 ccgtccggct cgcgaagctg gcgctgaaca acggtatcgc cggcgccctc gaaggcccga
      901 gctcgtacct gatgaagtcg ccgccgaagc aggtccccga cgacgaggcg tacgacgcgg
      961 ccgagaagtt catccgcgac aacgcccgca cctcctcacg cgtcgaggaa ggcgcaacca
     1021 caccgtagat cggcaaccct ttggagcgcg gcggacgtgt gtcacaatcc gccgcgcaat
     1081 ccaaggggag gagatgtgca ggctatgaag cgaccacttg tggtcctgct ggcgttgctc
     1141 gtcgtggcgg tggcggctac gactgccgga gccggcgaca acgactcagg gttcaagacg
     1201 agccaaccgg cgatgctgac tccgctggcg ccgggcgcga ccgtcacgcc gatcatcact
     1261 gtcggcgaca cggtcagggg ctaccgcttc gaggcgatcc cggacgggat ctcgctcgac
     1321 ccgaacggag aggggacggt cgacgtctac gtcaaccacg agacctcgac agtcccgttc
     1381 ccgtacacag cgggcgcggg ggccgcgaat caaaacgact tcgacaacgg ccagctcagc
     1441 aagctgcgcc tgaaccagca cagcgcggga gtgctcaagg gcgaactgac gatcacgagc
     1501 gcagagaact tccagcggtt ctgctcgaac ttcctcgcga cctcgaagga gggtttcgaa
     1561 cgttcgatcc tgttcacgaa cgaggagacg agcgacttcg tcaaccgcac gggacaagcg
     1621 tttccgcccg ttgccggtgc cgagcaggcc ggagtcgtca ttgcaaacga catccgctcc
     1681 gggcagcaca aggtgatcta cggaatgggc aggctgaacc acgagaacga cgtcgcggtt
     1741 gcgggatacg gccatccggt cgtcctttcc ggcgacgaca cgttcgtgag caaccctgcc
     1801 ggctcgcagc tgtactcgta catggcggac agcgccaacg ccctctggaa tgaccaaggc
     1861 gatctgtacg ggttcgtctc ggacaattcc gcagtggacg actactacga cttcccggtc
     1921 ggatcgtcga tgtccgtgag tgggcatttc gtgaagatcg acaagagtgc ggcgagcggc
     1981 gatcagaatg cgttggagtc ggcttccgac tcggcccacg tcttccagtt cgtgcgactc
     2041 gaggacatcc agtacgacaa gcgaccgggg atgcagaacg tcgtctacat cgccgacacc
     2101 ggcagaggct cgaacagcgc cagtggtaac gcgttcgcct cgacgaacgg ccgcatctgg
     2161 aagctcgtcc tcgaccccgg cgatccgacg aaggtgctct cgctctcggt ctatatcgag
     2221 ggcgacgacc agccggtgaa gacgatcggc gagatccacc agccggacaa cgtcgagacg
     2281 acgcagaacg ggctctacat caccgaggat ccgggctcga gccagcagtt tccggtcggc
     2341 tccaccgatc cgaacgcgac tcccgcacgt ctctggcggc acgacttcac gaccggggtg
     2401 gacgctccgg tggccgtggt gaaccagtcg gcggacgagg gccccaccga tgtcgactcg
     2461 tcgaccacgc tgggtgcctg gggctcctgg gagtcgacgg gtgtcatcga cgcgtcgtcg
     2521 gtcttcgggc cgggcgcgtt cctgatcaat gtccaggcgc acacgctctg ggtagagcag
     2581 tcggccgcgc ctgactggac gaacaagcgc gagggcggac agctcatgct cctgcgcatt
     2641 ccgggcgcgt aagccagtcg ggagggcctg gccatgacgg ccgggccctc ctcactacac
     2701 tcggtgcagg atgggtctca ggatctgcct cgtcacgccc tttgcctggt cgcagccgca
     2761 cgacgtcaac gagcacgtgg cgggcgtcgc aagggagctg cgcacacttg gtcacaccgt
     2821 gacggtcctc gcctcgtcga atcgggcccg agacctggct gcaggccgtc gcgccctgct
     2881 cgatgggctc gagtcggaag tgatcgcgct cggcccggca gtccccatct cgcgccggag
     2941 tcggatcggc gtgccggtcg gggcgcgtgc gaacctcgcg cttgcgctgg cactcggtcg
     3001 gttcgacgtc gtccacggct tcgagccggg gctcccttca ctctcgtatc tggcgctgcg
     3061 ggacgctcag gcgatgaccg tggcgacatt cttgaacgcc gaacggctcg gctatccgcc
     3121 cggacgagca cagcgcgacc gcctgcttgg gcgcatcgac gcgcttgtcg cgaccagcga
     3181 ggagacggcc gaggcggccg ccacccgctt tcccggccac taccgcgtcg tcagcgaggg
     3241 actcgagtct catctctttg agtcccggca caagcgcgac ctgatcgtcc tcgagtggcg
     3301 gccgaacgag cgcgctctcc tgcgcggagt gtttcgcgcg ctggaggagc tgccggactg
     3361 ggagctcgtc ctgctgcgca cgaaacccct ggccggccgg ccgacgatcc cgcgggtcct
     3421 gcgtgaccgc gtgcacgtgc gaacggcgcg ggatggtgcc gcacgagcgc cgatactcgc
     3481 ggaaacgagc atcttcgtcc cggcgctcgc ggggctccag cgcgtacggc tcgaagcatc
     3541 ggcgtccggg tgcgcgatcg cctccccacc cggcgtgcgc gaccagctcg agctcgccgg
     3601 cgccgaagtt gcacggcttg ccgaggaccc agagttccgg gcccgcaagg cggagcgagc
     3661 acgcgccgat gtcgtgggcc agagcttcgc ggacgtggcg cgcgagctcg acgagctcta
     3721 ccgctcgctg agcgggcgcc gtcattcggc tccgcgctcg gacgatccgc tcgccgatcg
     3781 gccgtgggtg ctctgcgatc tgcacatgca cacgagctgg tcgcccgact gtgcagtcga
     3841 cccggccgac ctggtcatgc atggggcggc gatcgggctc ggcgccatcg ccgtcacgga
     3901 tcacaacgtg ttcgggggcg cgctcgagac gaaggagctg gcacgcgacc acgagctcgt
     3961 cgtcatcccc ggcgaggagg tgaagacaga cgggcaggga gaggtgatcg gactgttcct
     4021 gcgtgaggag atcccccgcg gcctgtcctt cgccgacacg gtggcggcga tcaaggagca
     4081 ggacggcctc gtctatctcc cgcacccctt tgaccgcatg cattcgattc ccgatccggc
     4141 gacgctccag caccacctcg cggacatcga catcttcgag gtctacaacg cccgtctcct
     4201 cttcgaggcc tacaacgacg aggcgctgcg ctttgcgcgg aagtacaacc tgacgatggg
     4261 ggcgggctcg gacgcgcacg tgctccaggg ggtgggcacc ggcgccgtcc gcatgcgtgc
     4321 gttcgagggc ccggaggaat tcctcctcag cctccgctcg gcccaggttt tgcgccgccc
     4381 caggtcgctc gtgtacctcc agtccctcaa atggatggct caggccaagg aacgggttcg
     4441 gtagtcccga acctggtctg agcgtggcta cgacagacga gatctacgag aagtacctcc
     4501 agaaggcgat cgtcgagatc aacgacctcg cccacgaggt ggcccaggtg ggcggcggca
     4561 cgcgggtgcc ggtcgtcggg tccggccatc cgcttgccga cgtcatgctg ctcaaattca
     4621 ggccgcagcc ggccgaggta caggagggcg tcgccttcta cggccgcgcc ggccaggcaa
     4681 tcctgaaatc gctgcagcgg ctccatgtcg atcccatgtc cgtctacggg acgaattgcc
     4741 tcaagttcgc ggacggggaa gaggcggagt cgcggccgtt cctcacgcgc gagctgcaca
     4801 tcgtgcagcc gaagctgatc gtggcgatgg gagagagcgt cgtcgagttc ctcaacgcaa
     4861 tcgacttccc gcttgccgat accgtggagt acaagccggg cgagctcgag cgcttcaccg
     4921 caacggtcca ggtattggtt gtccccgaca tcgacgcgtc gctcgacgaa gcccccgcga
     4981 agactcagtt ttggaacgct ttcaagccac tcggccaatg gtgggccgag ctgccgccct
     5041 attaacgctc gggacggcgc tcgccctcta ctacgcgttc cacgacaggc tgtggaatgc
     5101 gtcgacgtgg tgggacatcg cgttcatcgc gttcgtcctc atccccgcct gcttcgcact
     5161 ggtgtggctc gtgctgcccc tacgcaatgc gcccggcttg ctcgcggtcg gcctcgcctt
     5221 cggcgtgttc gcgcttctgt ttcaccttgc gggttggaac acgccgggaa acttctgcaa
     5281 gctgttcggc gtcaccgccg tcggcttctg gttcctgcgc tacttcgaga cgctgaactg
     5341 ggtggtgctc gtcgccctga tcatcccctg ggtggatgcc tactccgtgt ggcgtgggcc
     5401 gacaaaggtg atcgtcaacg agcatcggaa cgtcttcacg aacttctcgt tcgccttcgc
     5461 gattcccggc gagacatcgg cagcgaacct cggcctgccg gatctcctct tcttcgcgct
     5521 cttcctcgcg gcctcggtcc agtgggagtt gcgtccacgg ttgacgtggc tgctgctgac
     5581 gctgtcgttc ggcgcgaccc tcgcactcgc ggtctggggc gagctcgggg gactccccgc
     5641 cttgccgctg ctcgcgttgg cgtttctgct cgcaaacggc gacctgatct ggacgcggct
     5701 caggcgggag cgggcagcac gctccgcgga aaccggaaca cgttcttctg gtcgtagcga
     5761 gccttgacga ttcgcaggcg cggcaggttg gtcgcgtagt aagagatgtt cggatgctcc
     5821 agctcggggt cgatgtagtt ctggtacgcc tgacctgaca cgtacggtgc cagcgcggcg
     5881 cggaacccac gcagccaatc ggcgtcagcc ggagtcgagc tcgccgcgac gtattgcaag
     5941 gagaagagct ggtcgcgatg cacgaatgcc gttgcgttct tcggaacgcg gttgatcgca
     6001 ccgccgtagg cgtccatcag gatgctgccg ccgccgagcg cgggatcgtt ctggcgctgc
     6061 tcgatcttct ggatcatcgc ttggattccg gcggctggga gcggcgtcgc aacataatcc
     6121 gacttggcca ggaagtcgga acggccgacc tccgcgcttg gcaccccgat atgacagcgg
     6181 gcgacatcgg aacagttcgc ccagtcgagg gcggcgttga tgaaggagcg cgtgcgaatc
     6241 gtcatcgtct gcggagttgc tgcgttcaac agcggttgca agagcgacgg cagcgcagcc
     6301 tcactcccga agtactggcc gttggacgcg acgattgggc cgccggggcc atcggtcgcg
     6361 atcaggtcgc acatcgagaa cagctcgtcc ggcgcagtcg gcgcccacgc ctgccaggcg
     6421 gcgacgacgg ctgcagcatc gctccagctc caggtcgcgc gataggtgac gacgtcgctc
     6481 gccgcatgcg tgtcgaagac gaagctcgtc acgatgccga actgccccgc gccgccgcct
     6541 cggcacgccc agtaaagatc cgggtgttgt gtctggctcg cgatcagcgc ctggccgctc
     6601 gcggtcacga tcgtgagccc gcggacattg tcgcaggtca gtccgtactt gcggccggaa
     6661 aatccgtggc cgcctccgag tgtcaggccg gcgatgccga cggtcgggca tgaaccggcg
     6721 ggaatcatca ctccctgctg gaacaacgcc tggtcgacgt cgatcagcct cgcccccgcc
     6781 ccgatcgtcg ccgttcctgc cgcgtggttc acggtgactc cgctcatccg cgtcacgtcg
     6841 acgacgacgc ccttgcaggt cgaataaccc gcgtaactgt ggccacccga tcgcgcgacg
     6901 acacggatcg agtgccgcct cgaccagcgg accgtgcgcc agacgtcctg ggccgtctcg
     6961 cagaagacga ccgcgccggg acggacgacg tcgtagcgcg ggctgtagag acgccgcgcg
     7021 gagtcatagg cggcgtccgc gggcgtcaca accgttccct ggacgagctg cgccagttcg
     7081 acgagtcgcg cgtcaacggt cgatcgcgcg ccgaccgtga tccaccctgg gagtgcggcc
     7141 accgcaccgg caccaacggc gagccgcccc ccgcggacga gcaactcgcg ccggtcgaac
     7201 ccctcagacc aggtgtcaga cactgtgccg gaagtgtgcc gtgcaattca cgaccccgcg
     7261 ataccccgct cagactcgga aactcatgcc aacgagttcc acaggcctgg tgtcagacac
     7321 cgtgccttag gtgtgacgtt ccagcagggc cacggcctcg acgtgcgggg tgtgcgggaa
     7381 catgtccacc ggcttcacgc gcttgagctc gtagccgtac tccgcgcgca ggcgcttgac
     7441 atcgcccgcc agggtcgtgg gattgcagct cacgtaaacg atgcgcggcg cgccgatctc
     7501 ccccaggcgc ttcagcgcct tgccagcgag ccctgcgcgc ggcgggtcga ccaccacgac
     7561 gtccggatcc ccggcgcgct cgtgcagctc ctccagcgcc tggccgacgt tgcctgcgaa
     7621 gtacgcggcg ttgccgatgc cgttcagctc cgcgttctcg agcgcgcacg cgatcgactc
     7681 ctcggagacg tcgatccccc agaccgtgag cgcatctcgc gcgagcagga gcccgatagt
     7741 gccgatcccg cagtagaggt cgtacaccgt ctcgtcgccg gtgagcgccg caaattctcg
     7801 cgccgtctca tagagcttct ctgccatcag cgtgttggtc tgcaggaacg cattcgggcg
     7861 aacgcggaaa cgcatcccgc acagctgctc ttcgatcgcc tcctctcccc agagcaactg
     7921 ggtggggaga ttggtcgtca cttccgccgg cgtgtcgttg atcgaccagt ggatcgaacg
     7981 cacctccggg aattgccgca gcgtgtcgac gaatccaccg gtgtcgaact tctcgccggg
     8041 gctcgtgacg agctgaacca ggacctggcc ggtattgcga ccctcgcgca cgacgaggtg
     8101 gcgcaggtaa cccgtgtgct cggcctggtc gtacgcgacg agcttgtcgc cgcgcgccca
     8161 ctcgcgcacg gcgttgcgga tcgcgttgcc gacgtcagtc gtcagccagc aatcgtcgat
     8221 ctgcagcacc tcgtcccagc gacccgccct gtgcaggccg agcgtcggcc cgtcttcgag
     8281 ttgtgtgaac gagtactcga gcttgttgcg gtaatggaag atttcctcgg ccggaaggat
     8341 ctcctcgagc ggcggctccg cgaacccgcc gatgcgccgg agcgcgtccc cgacctgagc
     8401 gtgcttcgct tcgacctgcg cctcgtaggc gagatcctgg aaccggcagc cgccgcaaga
     8461 cgggtagtgc ttgcagggag cttcgacgcg cagcggactg ggctgcagca cttcggtgac
     8521 gagcgcctcg gcgtgccgcc gctgcacttt ggtcacgcgt gctcgaacgg tgtcgcccgg
     8581 aaggccgcgt cgcacaaaga cgacgaagcc gttcaggcga gcaacaccgt tcccgccgaa
     8641 tgcgaggcgg tcgatggtga gttccagctc ctgatctttg gtaacgggcg cggccactgc
     8701 cgaccagggt atgcgctagg cggctttgcg gagccgtccc cggcaggcga ccggatggtg
     8761 tgtcgaggtc tggaaagccg tacgatcgct ctgtggatgc agaagcagcg gcgcgtgcat
     8821 gggtcgacgc ctgggaccgc gcgtggcggg caaaggacga gagcgcgctc gagccggtct
     8881 acgccgagga cgccgttcac cgctcgcatc cggaccgcga gccacactca ccaatcgagt
     8941 acgcgcggtc agcgttcgcc gacgagggcg acgacctcga gctctcgtgg ggcgaaccgc
     9001 tcgtcgccgg caaccgcgcc gccgtcgaat ggtgggcggc gctgacggag agcggcgagc
     9061 tcgtcacgct cgcgggcacc tcctggctcg cgttcggcaa caacggtcgc gtcgtcgagc
     9121 aacacgatta ttggacagtc acgcccgggc gctcagctcc ctggaaaggc tggggctcct
     9181 aactaagcgg ggtgacgtag accttccgct cgcgcggccc atcgaactcg cagaagaaga
     9241 tcccctgcca cttgccgagc gcgagcttgc caccgtcgag cggaatgagg atctgcgggc
     9301 tcatcagcga cgcacggatg tgcgacggcg cattcggccc gtccggatcc tcgacgtgct
     9361 tccagtccca ggcgtcgtcc accaccttct ccaacgccgc ctcgaggtcc tgcgcgacag
     9421 ccggatcagc cccttcgttg atcgtgacgc ccgcagtggt gtgcgggacg tagacgagcg
     9481 cggccgccgc gccgttgatc gagccgagcg cttcctgcac ctgctcggtg atgtccacaa
     9541 gctgggtgcg gcgctcggtc tggaggcgga tctcttccac ggcttgcgta tcctaggcgc
     9601 atgcacgtga acacgaggaa tggggcgacc caagatgctg gttgaggtgc atgcttcccg
     9661 caccgccctc tgaccgggag ctggacgcct atcgccaaca ggcggaccgg ttcatcgccg
     9721 agctcgacga ggagttctac ctccattacg ccggtttgaa ggagaccttc gacctggcgc
     9781 cgatctacga gcggcacaag aatctgaccg agctcgacca ggtcaagtcc atcggcctgg
     9841 ccgtcaacgg aggggcacgt gtgcgggaac tgtggcggtt cgcctgcgag gggtatttcg
     9901 gcgagctcac ccgcgatcac gccgagaagc tcgccgagct ggagtcggag ctcgcggtct
     9961 cggtcgacgg cgaggagatt ccgtatcgga tgatccgccc ggccctggcg aacgagccgg
    10021 accgggagaa gcgccgcgtg ctggacgaac gcgcgaacga gttgctcgac gagcacatga
    10081 acccgctcca cctcgacgcc gctcagactg tgcaacgggg gacgctcaac ctcgagttcg
    10141 cgaactatct ggagctctac cgcgaggctc tgccggcctc tgcgctcgac gacctcgctg
    10201 cgcaatgccg gacgttcctc gactcgacgg agcggctcta cgaggaatgg gccgacaaac
    10261 tcttccgcac gcgcgttggt gtctcgctct cggaggcgga gcgctgggac gtgccgcgct
    10321 tgttccgcgc gcccgagtgg gatccgatgt tccccgccga caagatggtc cccgcgctcg
    10381 agggcacgct cgaggacctg ggcatcgacc tgagctccca ggaaaacgtg accctcgacc
    10441 tcgaagagcg cccgaacaag agcccgcgcg ccttctgcgc acccatcgag gtccccgatc
    10501 gcgtcgtgct cgtcatccag ccgatcggcg gtcccgacga ctggcgcgcg ctcttccacg
    10561 aaggcgggca caccgaacac ttcgcccata cgtcgcggga cctctcgatg gaggagaagc
    10621 ggctcggcga caacgccgtc acggaaggct gggcgatgct gctgcagcat ctgaccgacg
    10681 agcccgagtg gctgacgcgc cgactggatt ttccgcgtcc caacgaatac gccgtcgaag
    10741 gcgccacggg cctgctgtac ttcgtgcgcc gctacgccgc gaagatcctg tacgagctcg
    10801 agttccatgc cgcagaggac gtgacgacga tgcgcggccg ctacgcggag ctgctctccg
    10861 acgccctgaa gatcgaagtc acccccacga attacctcgc cgacatcgac tccggcttct
    10921 atgtgtcgtc gtacctgcgc tcgtgggcgt tcgaggctca gctgcgcgcg tacctgaagg
    10981 agaagttcgg ctcgaagtgg ttcgccacgc gcgaggccgg atcgctgctg cgcgagctct
    11041 ggggcgaagg ccagaggatg cgcgccgagg agatgctgaa ggatgtcacc ggctcgactc
    11101 tggagatgga ggcagtcgcc gagcgggtga aagaggttct tcggtagtcc atcctggctc
    11161 catgcataag ctttatgcat ggcgagaacc cgagtcctga tcgccggcgc cgccggccgc
    11221 gacttccaca acttcaacct cgtctaccgc gacagtccgg cacacgatgt ggtcgcgttc
    11281 accgcgactc agattccgaa tatcgacggt cgccgctacc ccgccgaact ggcggggtcg
    11341 ctgtacccgg aaggcattcc gatcctgccc gaatccgcgc ttgagcaact cgtgcgtgag
    11401 cacgagatcg acgtggtcgt cttcgcgtac tcggatgtca cgcacgagca cgtcatgcac
    11461 atcggctcgc gcgcgctcgc cgcgggcgct tcgttcgagc tgatcgcgcc acgggagacg
    11521 atgctcgcct cgtcgaagcc gaccgtggcg atttgcgcgg tgcgcacggg ctcgggcaag
    11581 agccagacga ccaggcgtgt ggcagaactg ctccgcgaag caggcaagcg cgtcgccgtc
    11641 ctgcgccacc caatgccgta cggggatctg acgaagcagg ccgtgcaacg gttcgagcgc
    11701 tacgaggatc tcgaggcagc ggacgcgacg atcgaggagc gcgaggagta cgagccgcat
    11761 ctcgccgagg gcaacctcgt cttcgccggc gtcgattacg agcgcatcct gcgagctgcg
    11821 gagcaagagg cggacgtcat cctctgggac gggggcaaca acgacacgcc gttcatcaag
    11881 cccgacctgc acatagtcgt cgtcgaccca caccgcccgg gacacgagct ccgctaccac
    11941 cccggtgaga cgaacctccg catggcggac gtctgcatcg tcaacaagat ggacagtgcg
    12001 tcacaggagg gcatcgacgc ggttctcagc tcgattcacg agaacaacgc ggacgcacgc
    12061 atcgtcctgg cggcgtcgcc cttccatgtc gaaggcgacg ccgaggagat caggggaaaa
    12121 cgcgtgctcg cggtggaaga cggaccgacg ctgacacacg gggagatgac ctacggcgcg
    12181 gcagtcct
//
LOCUS       DASVEU010000156         6616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_691459,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000156 DASVEU010000000
VERSION     DASVEU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691459"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF225_06220"
     CDS             complement(<1..420)
                     /locus_tag="VF225_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7144888.1"
                     /translation="MAHKELKFNEDARRALERGVNILADAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIEVEDVFENQGAQLVREVATSTNDVAGDGTTTATVLAQAIVKD
                     GLRNVAAGANPMAIKRGIERSVDAVVENLKSQSKEISG"
     gene            complement(446..763)
                     /gene="groES"
                     /locus_tag="VF225_06225"
     CDS             complement(446..763)
                     /gene="groES"
                     /locus_tag="VF225_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7144889.1"
                     /translation="MNLQPLGDRLIVEVLEEEEQTISGIVLPDTAKEKPQRGRVLAVG
                     PGARDEDGKHIPMDVAEDDEIIFSKYGGTEIKVGADEVLILRESDVLAKVVGDRELAG
                     AKA"
     gene            920..1324
                     /locus_tag="VF225_06230"
     CDS             920..1324
                     /locus_tag="VF225_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7144890.1"
                     /translation="MVVRNLRGRPVVAVIRPGGKPEGTWALPKGLIGPGERAEVTALR
                     EVREETGVEATPVEKLGDVRYVYTWAGERVFKVVSFFLFRYRAGRLGDIPPEHAHEVA
                     ETRWLPLEEAPRLLAYKGEREMAAKALERLSR"
     gene            1335..1634
                     /locus_tag="VF225_06235"
     CDS             1335..1634
                     /locus_tag="VF225_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX7144891.1"
                     /translation="MSEPEKEAPVLEAESRMRRGIRYSHRTGLYASLVVAIATIVVLI
                     LLIAQNTRRVKVDYVFGTSQTRLIWLVIVSAITGWVLGIVTAFLIRRRTRWRGPT"
     gene            1631..1990
                     /locus_tag="VF225_06240"
     CDS             1631..1990
                     /locus_tag="VF225_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX7144892.1"
                     /translation="MTEAMYALNFYSPIVAEQLRSGRKTATIRLGDKSAKYRKGMIVQ
                     VLAGARFSPRDRVFAAVIDKVEVKMLSELSPREIEHDNPEIRRTEDMASFLSQLYNRE
                     VTPDDTVTVIRFSQIKT"
     gene            complement(2135..2407)
                     /locus_tag="VF225_06245"
     CDS             complement(2135..2407)
                     /locus_tag="VF225_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144893.1"
                     /translation="MGKASDWLREERRKVLGDWVAFCLGCGAARRWFDDFEAEVPEEC
                     PQCGGRMLRRCPSCSAPFSSTFAVDCESCGARLREPELFGTKIRRR"
     gene            complement(2407..3012)
                     /locus_tag="VF225_06250"
     CDS             complement(2407..3012)
                     /locus_tag="VF225_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144894.1"
                     /translation="MQNERFSHNRFLVDQLIRPMINLYRVTPLAAGETPTGGPIAFVR
                     QKRMAIKEDIRFFADEQETEELFRIKARSVFDIGGARYDVTEPDGTPIGVLEHAFGKS
                     LLRSTWKVTGADGSLVTAQERSVPMAIARRVVDFVPYGEWLPIPYNFDLLMDGRLIGH
                     MNRKFQLRDRYILDLSADHELKLDRRLGIALAIGLDTLQNR"
     gene            3052..4098
                     /locus_tag="VF225_06255"
     CDS             3052..4098
                     /locus_tag="VF225_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7144895.1"
                     /translation="MAIAREAEPVGSLREVREAGLLREDLLGVYRNMVMSRGIEERGH
                     ILYRQGKIPGSFYTGRGNEGAAVGVATAMGPDDVGTPLHRDMGVHVVRGVEPWRIFAQ
                     FMGRADGPTKGRDGNVHMADSKLGLIARVSHLPAMLPVAVGCALAFRIREEKRVAVGW
                     FGEGASARGDTHESMTLAGVRKLPMVFICDNNQWAYSTPTHLEFATQHVADRADAYGF
                     EGIVVDGTDVLAVYREAKRAIEKAREGGGPTLIECVTLRMEGHAVHDDAFYVPKELFE
                     KWAENDPIERFRTWLRTNADMSDEEEDEITGEVKKTLNDALKRAEESPMPDPATLLEG
                     VYATPEDLDTPHHK"
     gene            4098..5072
                     /locus_tag="VF225_06260"
     CDS             4098..5072
                     /locus_tag="VF225_06260"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7144896.1"
                     /translation="MAELTYLQAISDGLRTEMRRDKRVFVIGEDVGVYGGAFKVTLGF
                     QEEFGHWRVLDAPLAETAIVGACTGAAIMGMRPVAEMQFADFISCAWDQLVTVAAKQR
                     YRAGTPVPITVRLPSGGGFSGGPFHSQNPESSFAHIPGLKCVCPATPEDAKGLLISAI
                     EDPNPVLYFEHKHLYRRIKGEVPEERYTVPLGKARTHRDGKDISVITWGAMVYTAEEA
                     AQQLDTDGVSVEIIDLRTVMPWDKAAVLESARKTSKVLVLHEDTRTGGFGAEIAATVA
                     EEAFEDLDAPVKRIAAPDAPVPFSPPLEKAFIPQVEDVTAGLRELAEY"
     gene            5082..6482
                     /gene="sucB"
                     /locus_tag="VF225_06265"
     CDS             5082..6482
                     /gene="sucB"
                     /locus_tag="VF225_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX7144897.1"
                     /translation="MATTEAIDVVMPQMGVSVSEGTVTKWLKQEGEQVEADESLLEIS
                     TDKVDTEVPSPASGTLTQILVQEGETVDVGTKLAVIGGDGAADAPKEEVQVPEPATAQ
                     AAAESAAVSDAPATPPTADTVEEATPAPAAASETTDAPSTNGKTFVSPVVAKIASEHG
                     VDPSQVHGTGRGGRVTKKDILDFIESGAQAAPPATPAQPEAPKAPVPAPAPEAPAAAP
                     APPVAAPAVSQPGESFEPITAMRRGIAEHMRRSLDTSAHVTSAIEVDMSKVSAIRAKL
                     KQEYQQNYGVNPTYLIFVARAATDTLREYPWINGELRGEQIVTRSYVNLGFAVELQDG
                     KGLIVPVVKNAETLNLLGMAKAVTDIAQRARDKKLLPDEVQGGTFTITNPGGYGTFHG
                     TPVISQPQAGILGTYAVVKRPWVIQDDLGEDVIAIRPIMNLTLTYDHRLVDGALAGRF
                     LRDLRHKLETWDESAY"
BASE COUNT         1140 a         2217 c         2207 g         1052 t
ORIGIN      
        1 gcctgagatc tccttggact gtgacttcag gttctcgacg acagcgtcga cggaacgctc
       61 gatgccgcgc ttgatcgcca tcgggttggc cccggcggcg acgttgcgga gaccgtcctt
      121 gacgatcgcc tgagccagca cggtcgcagt cgtggtgccg tcgccggcga cgtcgttcgt
      181 cgacgtagcg acctcacgga cgagctgtgc gccctggttc tcgaagacgt cctcgacctc
      241 gatctcgcgt gcgatcgtga cgccgtcatt cgtaatcgtg ggcgcgccga acttcttgtc
      301 gagcacgacg tagcgcccct tcgggccaag ggtgaccttg accgcgtcgg cgaggatgtt
      361 cacgccgcgc tccagtgcac gacgggcgtc ctcgttgaac ttcagctcct tgtgagccat
      421 atgccttcga tacctccgtt attccttagg ccttcgcgcc cgcgagttcg cggtcgccga
      481 caacttttgc gagaacgtcc gactcccgga ggatcagcac ctcgtcggcg ccgaccttga
      541 tctccgttcc gccgtacttg ctgaagatga tctcgtcatc ctcggcgacg tccatgggaa
      601 tgtgcttccc gtcttcgtcc cgagcaccgg gacctacggc gaggacgcgg ccgcgttgcg
      661 gcttctcctt tgccgtgtcg ggcagcacga tgccgctgat ggtctgctcc tcttcctcga
      721 ggacctcgac gatcaggcgg tcaccaagcg gctgcaagtt catccactct cctcctgttc
      781 agacgacttt gtgacatcta ctggcactct cggggtgaga gtgccgaggg attgtaccgt
      841 gccttggccc gggtctgcaa ggcaacacgt accactgtgt tacaaagtct tggtgcgccg
      901 cgagttctcc gctgggggtg tggtcgtccg gaaccttcgc gggcgtcctg tggtggccgt
      961 catccggccc ggggggaagc cggaggggac gtgggccctg ccaaaggggc tgatcgggcc
     1021 tggtgagcgg gccgaggtga ccgctctgcg ggaggtgcgc gaggagaccg gggtggaggc
     1081 aacgcccgtc gagaagctcg gggacgtccg ttacgtctac acctgggccg gcgagcgggt
     1141 cttcaaggtg gtcagcttct tcctgttccg ctaccgcgcc ggccggctcg gcgacattcc
     1201 gcccgagcac gcccacgagg tggcggagac gcggtggctc cccctcgagg aggcgcccag
     1261 gctgctcgcc tacaagggcg agcgggagat ggcggcgaag gcgctcgagc gcctctcgcg
     1321 ttaggctccc gcgcgtgagc gagccggaga aggaggcgcc tgtcctcgag gcggagtccc
     1381 gcatgcggcg ggggatccgc tactcgcacc ggaccgggct ctacgcctcg ctcgtcgtgg
     1441 cgatcgcgac catcgtcgtc ctgatcctcc tgatcgccca gaacacgagg cgcgtaaagg
     1501 tcgactacgt cttcggcacc agccagacgc ggctgatctg gctcgtcatc gtcagcgcga
     1561 tcacgggctg ggtgctcggg atcgtgacgg ccttcctcat ccgccgccgc acgcgttggc
     1621 gcgggccgac gtgaccgagg ccatgtacgc gctcaacttc tactccccga tcgtcgcgga
     1681 acaactgcgg agcggacgca agacggcgac gatccggctc ggggacaagt cggcgaagta
     1741 ccgcaagggg atgatcgtcc aggtgctggc cggcgcgcgc ttcagtccgc gggatcgggt
     1801 gttcgcagcg gtgatcgaca aggtcgaggt gaagatgctc tcggagctgt ccccgcggga
     1861 gatcgagcac gacaaccccg agatccgccg caccgaggac atggcctcct tcctcagcca
     1921 gctctacaac cgcgaggtca cgcctgacga cacggtgacc gtgatccgct tctcgcagat
     1981 caagacctga cgcactcgtg tcacacgggc gtacccgtgc ctggcaccgg tacgtaacct
     2041 cagacgccgc gcccctgcgg cagcgctgcg aaacccgaga cagcctcgtt ccagtttcgt
     2101 acccgtgcct ggcaccggta cgtgcccgaa ggagctagcg gcggcggatc ttcgttccga
     2161 agagctccgg ctcccgcagc cgggcgccgc acgactcgca gtccacggcg aatgtcgagc
     2221 tgaagggggc cgaacacgac gggcagcgac ggagcatcct cccgccgcac tgcggacact
     2281 cctccgggac ctcggcttcg aagtcgtcga accagcgccg cgcggcccca cacccgaggc
     2341 agaacgccac ccagtcgccg agcaccttgc ggcgttcctc acggagccag tcggaggctt
     2401 tgcccatcag cgattctgga gcgtgtccag tccgatcgcc aacgcgatcc cgagccgccg
     2461 atcgagcttc agctcgtgat ccgcgctcag gtcgaggatg tagcggtcgc gcagctggaa
     2521 cttcctgttc atgtgaccga tcagccggcc atccatgagc aggtcgaagt tgtacgggat
     2581 cggcagccat tccccgtacg ggacgaagtc gaccacgcgg cgcgcgatcg ccatcgggac
     2641 gctgcgctcc tgcgcggtca cgaggctgcc gtccgcaccg gtcaccttcc aggtcgagcg
     2701 gagcagcgac ttaccgaagg cgtgctcgag aacaccgatc ggcgtgccgt ccggctcggt
     2761 cacatcgtag cgggcgccgc cgatgtcgaa caccgagcgc gccttgatgc ggaagagctc
     2821 ctccgtctcc tgctcgtcgg caaagaagcg gatgtcctcc ttgatcgcca ttcgcttctg
     2881 ccgcacgaac gcgatcggcc caccggtcgg cgtctccccc gcggccagcg gcgtgacccg
     2941 atagaggttg atcatcggcc ggatcagctg gtcgacgagg aagcggttat gggaaaaacg
     3001 ctcgttctgc acggaggcga tggtaacgac agtcgttaga gtgcctgcgc catggcgatc
     3061 gcccgggaag ccgagccggt tggttctctg agagaggttc gcgaggcggg cctcctccgc
     3121 gaggacctcc tcggcgtcta cagaaacatg gtgatgagcc gcggcatcga ggagcgcggc
     3181 cacattctct accgccaggg caagatcccc ggctcgttct acaccggtcg cggaaacgaa
     3241 ggcgccgccg taggggtggc caccgcaatg ggccccgacg atgtcggcac gccgctgcac
     3301 cgcgacatgg gcgtccacgt cgtgcgcggc gtcgagccct ggcggatctt cgcgcagttc
     3361 atgggccgcg cggacggccc gacgaagggc agggacggca acgtccacat ggcggactcc
     3421 aagctaggcc tgatcgcgag ggtcagccat ctgcccgcga tgctcccggt cgcggtcggc
     3481 tgcgcgctcg ccttccgcat tcgcgaggag aaacgcgtgg ccgtgggctg gttcggcgaa
     3541 ggcgcttctg cacgaggcga cacccacgag tcgatgaccc tggccggcgt gcgcaagctg
     3601 ccaatggtct tcatctgcga caacaaccaa tgggcgtatt cgacgcccac gcatctcgag
     3661 ttcgcgacgc agcacgtcgc cgaccgcgca gacgcctacg gcttcgaggg aatcgtcgtc
     3721 gacggcaccg acgtgctcgc cgtctaccgc gaagcgaagc gcgcgatcga gaaggcgcgc
     3781 gagggaggcg gaccgactct gatcgaatgc gtcacgctcc gcatggaagg ccacgccgtg
     3841 cacgacgacg ccttctacgt tccgaaggag ctgttcgaga agtgggcgga gaacgacccg
     3901 atcgagcgct tccggacgtg gctgcgcacg aacgcggaca tgtcggacga ggaggaggac
     3961 gaaatcacgg gcgaggtcaa gaagaccttg aacgacgccc tcaagcgggc cgaggaatcg
     4021 cccatgccgg atccggcgac actgctggaa ggcgtctacg caacgccgga agatctcgac
     4081 acgccacacc acaaataatg gccgagctca cgtacctgca ggcgatctcc gacgggctcc
     4141 gcaccgagat gcggcgcgac aagcgcgtgt tcgtgatcgg cgaggacgtc ggcgtctacg
     4201 gcggcgcctt caaggtcacg ctcggcttcc aggaggagtt cgggcactgg cgcgtcctgg
     4261 atgcgccgct ggcggagacg gcaatcgtcg gcgcctgcac aggcgcggcg atcatgggca
     4321 tgcgccccgt tgcggagatg cagttcgcgg acttcatctc atgcgcttgg gaccagctcg
     4381 tcacggtcgc tgcgaagcag cgctatcgcg ccgggacgcc ggtgccgatc accgtgcgcc
     4441 tcccctcggg cggcggcttc tccggcggcc cgttccattc gcagaacccg gagtcttcgt
     4501 tcgcgcacat cccggggctc aagtgcgtct gtccggcaac tccggaagat gcgaaaggat
     4561 tgctgatcag tgcgatcgag gatccgaacc ctgttctcta tttcgagcac aaacacctct
     4621 accgtcgcat caagggcgag gtgcccgagg agcgttacac ggtaccgctc ggcaaggcgc
     4681 gcacgcaccg cgacggcaag gacatctcgg tgatcacgtg gggcgcgatg gtgtacacgg
     4741 ctgaagaggc ggctcagcag ctcgacacgg acggcgtgtc ggtcgagatc atcgacctgc
     4801 gcacggtcat gccctgggac aaggccgccg tgctcgaatc cgcgcgtaag acgtccaagg
     4861 tgctcgtgct gcacgaggac actcgcaccg gcggtttcgg cgccgagatc gcggccacag
     4921 tggcggagga ggcgttcgag gatctcgacg caccggtcaa gcgcatcgcc gcgcccgacg
     4981 ctccggtccc gttctcgccg ccgctcgaga aggcattcat cccacaggtt gaggacgtca
     5041 ccgcgggcct gcgcgagttg gcggagtact aaaggcgaga gatggcaacc accgaggcaa
     5101 tcgacgtcgt catgccgcag atgggcgtgt ccgtttccga gggcaccgtc accaagtggc
     5161 tgaagcagga gggtgaacag gtcgaggcgg acgagtcgtt gctcgagatc tcgaccgaca
     5221 aggtcgacac cgaggtgccg agcccggcct cggggacgct gacgcagatc ctcgttcagg
     5281 aaggcgagac cgtcgacgtc ggcacgaagc tcgcagtcat cggcggcgac ggggccgctg
     5341 acgcgccgaa ggaggaggtg caggtgcccg agcccgcgac cgctcaggcc gcagccgaat
     5401 cggctgccgt ctccgacgcg ccggccacac ctccgaccgc cgacacggtg gaagaggcca
     5461 cgccggcacc cgccgcggcg agcgagacca cggacgcgcc gtccaccaac ggcaagacct
     5521 tcgtctcgcc cgtcgtcgcc aagattgcgt ccgagcacgg cgtcgatccg agccaggtgc
     5581 acggaaccgg gcgaggcggc cgcgtcacca agaaggacat cctcgacttc atcgagtccg
     5641 gcgcgcaggc tgccccgccg gccacgcccg cgcagcccga ggcgccgaag gcaccggtcc
     5701 cagccccggc ccccgaggcc ccagcggccg ctccggctcc gccggttgcg gcgcctgccg
     5761 tgtcacagcc tggcgagagc ttcgagccga ttaccgcgat gcgcagaggc attgccgagc
     5821 acatgcgccg ctcgctcgac acctcggcgc acgtcaccag cgcgatcgag gtcgacatgt
     5881 cgaaggtatc ggcgatccgg gcgaagctga agcaggagta ccagcagaac tacggcgtca
     5941 acccgaccta cctcatcttc gtcgcacgcg cagccaccga cacgctacgg gagtacccct
     6001 ggatcaacgg cgagcttcgc ggcgagcaga tcgtgacgcg aagttacgtc aacctcggct
     6061 tcgcggtgga gctgcaggac ggcaagggcc tgatcgttcc ggtcgtgaag aacgccgaga
     6121 cgctgaacct gctcgggatg gcgaaggccg tgaccgacat cgcgcagcgc gcgcgcgaca
     6181 agaagctgct gcccgacgag gtgcagggcg gcacgttcac gatcacgaat ccgggcggct
     6241 acggcacgtt ccacggcacg cccgtgatca gccagccgca ggctgggatc ctcggcacat
     6301 acgcggtcgt caaacgtccg tgggtgatcc aggacgatct cggtgaggac gtgatcgcaa
     6361 tccgcccgat catgaacctg accttgacgt acgaccaccg cctggtggac ggcgccttgg
     6421 ccggacgctt cttgcgcgac ctgcgccata agctcgagac ctgggacgag tcagcctatt
     6481 gaccgagcga aagctgcgct ttcgctcggg aattgggaac ggcgcttcgc gctgaacgtg
     6541 cgcgggccga gcccgcgcac tcctttcgtg ttagtgctcg gcgattgttc ccttccgtcc
     6601 gggcccgggc gctgaa
//
LOCUS       DASVEU010000157         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_305654,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000157 DASVEU010000000
VERSION     DASVEU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305654"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..131
                     /locus_tag="VF225_06270"
     tRNA            58..131
                     /locus_tag="VF225_06270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Met,seq:cat)
     gene            complement(248..712)
                     /locus_tag="VF225_06275"
     CDS             complement(248..712)
                     /locus_tag="VF225_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144898.1"
                     /translation="MRKLWLPALVGVAAAALTAGASADPGTHSKLTAITGQGAGIVEV
                     APTAHDVVGPDTFDVQGTVNVHHAMPNTTFTVLRRVDLNPDGVCTGAMWLTLPPPNEQ
                     TMTTSNGGAGALHFRISRGAPFVDGVRFDVEWQLVGSDGSVLQSDCFTVTVK"
     gene            806..2401
                     /locus_tag="VF225_06280"
     CDS             806..2401
                     /locus_tag="VF225_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7144899.1"
                     /translation="MLCLLGPPAIAKAKGLQPLALRPKALALLARVALSDEKQERRVL
                     AELLFHEAVDPRDSLRWHLSYLRVRVPDALVVERNAVSVRVPTDVVAFRSGARRIFGD
                     PASPDAQETLALYRGDLCAGLQVTASADFHNWLYVQEDELRRTFRRAAVAYARAALAD
                     GRAATAIPSLRRLTDIDPYLEEGHVLLVRASEAAGQWGDARHAYDRYQRIVRTELHAE
                     PRRELALRYEPERPAGRGLPLDELVPLEEITMHVVDWPGEEPAVVAIHGSAGHGYALT
                     ALGERLAPDVRFIALDLRGHGFSDKPPSGYGVEEHVEDVVQLIDALGLERPILLGHSI
                     GGAIATFAVEADRDAFGGLILFDAVVGDRRFVESASIVVDEWGPSLEQRFAAFDEYHG
                     RWGAEPEDSTWKRWLERSDRMELAPLPDGTLRRRGLRQALATEWASVARADALGALSR
                     VAVPVLVVYAHAAWDGAPYLDEATVDAQLAAARDSRLYVASGQDHADIVRRPSDGLVS
                     ALKDFVKYVRARTPATQAESERR"
BASE COUNT          424 a          935 c          952 g          464 t
ORIGIN      
        1 agctgccccg tcggcctcgt ccgttcggcg taagaggcgc tacaatcccg ccagcgacgc
       61 ggggtggagc agtcaggtag ctcgccgggc tcataacccg gaggtcgcag gttcaaatcc
      121 tgcccccgct attgagcccc cgcgtttgcg ggggtttttc tttggaggac gctatgaacc
      181 tgcgaccagc ggggtgagca tcggccgctc gcccaccgcg acaggcgggc gagcggccgg
      241 tgcacggcta cttcaccgtg acggtgaagc agtcgctctg gaggacggag ccgtcgctac
      301 cgacgagttg ccactctacg tcgaagcgga cgccgtccac gaacggcgcg ccgcgcgaga
      361 tccggaagtg aagcgcgccg gcgccgccgt tcgacgtcgt catcgtctgc tcgttcggcg
      421 gcgggagcgt cagccacatc gcgccggtgc agacgccgtc cgggttgagg tcgacgcgac
      481 gcaggacggt gaatgtcgtg ttcggcatcg cgtgatggac gttgaccgtg ccctggacgt
      541 cgaacgtatc cgggccgacc acgtcgtgtg cggtcggtgc gacctcgacg atcccggccc
      601 cttggccggt gatcgcggtc agcttgctgt gcgtgcccgg atccgcggat gcgcctgcag
      661 ttaacgcggc ggccgcgact ccgactagag cgggcagcca aagcttccgc atgagtagct
      721 cctttcagcc gtgtaccgga ggcacgaggc taggccggcc ggcgggcgca cacgttcgcg
      781 cacacgatcg ttcgataatc ggtccgtgct gtgtctgctc ggcccgcctg cgatcgcgaa
      841 ggcgaagggc cttcaaccgc tcgctctgcg gccgaaggcg ctcgctctgc tggcgcgagt
      901 ggcgctcagc gacgagaagc aagagcgtcg ggtcctggcc gagctcctgt tccacgaggc
      961 cgtcgatccg cgcgacagcc tccgctggca cctgagttac ctgcgcgtcc gcgtgcctga
     1021 cgcactggtt gtggagcgca acgccgtttc ggttcgcgtg ccgaccgatg ttgtggcgtt
     1081 ccggtcggga gcgcggcgga tcttcggcga ccccgcttcg cctgacgcac aggagacgct
     1141 tgcgctgtac cgaggtgacc tttgcgccgg cctgcaggtc accgcatcag ccgacttcca
     1201 caactggctc tacgtccagg aggacgagct gcgccgcacg tttcgacgtg cggcggtcgc
     1261 gtatgcgcgc gctgcgcttg ccgacggccg tgcagctacc gcgatcccct cgctccgacg
     1321 gctgacggac atcgacccgt atctcgagga gggtcacgta ctgctcgtta gggcgagcga
     1381 agcggcggga cagtgggggg acgcgcggca cgcctacgac cgctatcagc gcatcgtccg
     1441 cacagagctc catgccgagc cacggcgaga gcttgcgctc cggtacgagc ccgagcggcc
     1501 ggccgggcgc gggctgccgc tcgacgagct cgtcccgctg gaggagatca cgatgcacgt
     1561 cgtcgactgg ccgggcgagg agcccgcagt cgtcgcgatt cacggctccg ccggacacgg
     1621 ctacgccctg acggccctcg gcgagcgtct cgccccggac gtgcgcttca tcgctctcga
     1681 cctacgagga cacggctttt ccgacaagcc gcctagcggt tacggagtcg aggagcacgt
     1741 cgaagacgtg gtgcaactga tcgacgccct cgggcttgag cggccgattc tgcttggcca
     1801 ttcgatcggc ggcgcgatcg caacgttcgc ggtcgaagca gatcgggacg cgttcggcgg
     1861 cctgatcctg ttcgacgccg tcgtcggcga ccgccggttc gtcgagagcg cctcgatcgt
     1921 tgtcgacgag tgggggccgt cgctcgagca gcgattcgca gccttcgacg agtaccacgg
     1981 tcgctggggc gcggaacccg aggactccac ctggaagcgc tggctcgagc gcagcgaccg
     2041 gatggagctc gctcccctgc cggatggaac gctgcgccgg cgcggcttgc gccaagcgct
     2101 cgccacggag tgggcatccg ttgcacgagc ggacgcgctg ggcgcgctct cgcgggtagc
     2161 cgttccggtg ctcgtcgtct atgcacatgc agcttgggac ggagccccgt acctggacga
     2221 ggccacggtc gacgcacaac tcgcagccgc tcgcgattcg cggctctatg tcgcgtccgg
     2281 gcaggaccat gcggacatcg ttcgcaggcc gagcgacggg ctcgtgagcg ctctcaagga
     2341 tttcgtgaag tacgtccggg ccaggacgcc cgcgactcaa gcagaatcag agcgtcgcta
     2401 atggatccat atcgcgcccc cgctactgga aaggccccgg aaacggggcc ttttgcattt
     2461 gagccgcccg ccgcaggcgc aaacttttgc ccaacttttg cccgccgggt tcccgcatgc
     2521 gaatctgagc gccgcgagcg cgccgatttc gggccctacc cttcgcactg gtccggctcg
     2581 acggggactg tgagcacgcg attgccaagg gtgctccgag tggtactgcc cgcgacgacg
     2641 catgtccccc gtgctgtctt cggcacggaa tacgactggc aggcgttcca ctacttcacg
     2701 gaggtcaagg acgctcgacc gatcagggtg gcttcgcgcc gctacgagca agaccaagaa
     2761 gcgatcccgg cgctt
//
LOCUS       DASVEU010000158         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1275239,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000158 DASVEU010000000
VERSION     DASVEU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275239"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1138)
                     /locus_tag="VF225_06285"
     CDS             complement(236..1138)
                     /locus_tag="VF225_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7144900.1"
                     /translation="MAGGTRETDACSDDEPLCERPVEAVLRRLARAVRFRDEETAEHV
                     ERMSGSCALIAKELGWDQSQCAALRAASALHDIGKVGVPDSVLRKRGRLNARERTLVE
                     RHAEIGYEILSGSGDDVLELGARVALSHHERIDGIGYPRRLSGTDIPIEGRIAAVADV
                     FDSLIHDRVYRPAFAAEEALDMMRRGRGSQFDTEVFEAFERVLDEVLEISRRYPDGVT
                     LNVSSAGEGVVGSRVVGKLTPREREVVSLLALGLTGEEIATRLFISPETVRTHIRNAM
                     QRIGATTRAHLVMLAIARQEIEHP"
     gene            complement(1239..1826)
                     /locus_tag="VF225_06290"
     CDS             complement(1239..1826)
                     /locus_tag="VF225_06290"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7144901.1"
                     /translation="MVAARGQGRRLKSVFARSPVPMVMVDDERRYVEVNQPARFAFRL
                     SLAEMRKRRIDSLTPRESWPLMEAAWSRLIDAGCVAGPYEVQSPDGSRWEALYYGLAN
                     ALPGLHVIAFAPATALDIDGDEQELPEHVASLTGREVELLQLAAEGLTGPRIAEELAL
                     SPATVKKHFEHIYAKLAVGDRAAAVATAMRHGLIS"
     gene            1945..>2279
                     /locus_tag="VF225_06295"
     CDS             1945..>2279
                     /locus_tag="VF225_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7144902.1"
                     /translation="MRKAAEKNGIGARVRSARTRLGWTREALAFHSGLSWSAIAQVES
                     GRRTNLRPDTLFALGTALGVTIDYLVHGGVSSPVMFRHRALLYGTDRGFADAVVPCDR
                     VQGARRRGQP"
BASE COUNT          349 a          776 c          771 g          383 t
ORIGIN      
        1 gacgcgaaat ccgttcccat gaacgttccc acgcgtctcg gtcgcgggct agttcagcat
       61 ctctacgcgg gatcgcgggc ggatgcggag ggactcgaaa accgttgggg gccgttaggt
      121 ccctcgtggg ttcgaatccc accccctccg ctctacgagc ccaattccag cgccgatgtg
      181 gatcgccgtc ggtttctagc gcggctgccg aaagccactc gccagggctc agtcgtcagg
      241 ggtgttcgat ctcttgccgc gcgatcgcga gcatcacgag gtgagcgcgt gtcgtcgcgc
      301 cgatccgttg catcgcgtta cggatgtgcg tgcgcacagt ctcgggggag ataaacagcc
      361 gcgttgctat ctcctcgccc gtcaggccga gcgcgagcag gctgacgacc tcgcgctcgc
      421 gcggagtgag tttgccgacg acgcgggagc caacgacgcc ctcgcccgct gaactcacgt
      481 tcaaagtcac tccgtccgga tagcggcgcg agatctcgag cacctcgtcg agtacccgct
      541 cgaatgcctc gaagacctcg gtgtcgaact ggctcccgcg gcctcgccgc atcatgtcga
      601 gcgcctcctc cgcggcgaat gcgggccggt acacacggtc gtgtatcagc gaatcgaaga
      661 cgtccgcgac cgccgcgatt cggccctcga tcggaatatc ggtgccgctc aaccggcgcg
      721 ggtagccgat gccgtcgatg cgctcgtggt gactgagcgc gacgcgcgcg cccagctcca
      781 acacgtcgtc gccggagccc gagaggattt cgtagccgat ctcggcgtgg cgttcgacca
      841 gggtgcgctc ccgcgcgttg agccgacccc gcttccgcag gacgctgtcc ggcacgccga
      901 ccttgccgat gtcgtgcagc gcgctggcgg cgcgcagcgc ggcgcattgg ctctgatccc
      961 agcccagctc cttggcgatc agcgcgcatg agccgctcat gcgctcgacg tgctcggcgg
     1021 tctcctcgtc gcggaaccgc accgcccgcg cgagtcggcg caggacggcc tcaaccggtc
     1081 gttcgcacaa cggttcgtcg tcgctgcacg cgtcggtctc ccgcgttcca cctgccatgc
     1141 cgacaatttc gggccgggcg acgactccgg caatgggccg aacgcctgat ttcgtcgtga
     1201 gggatacgcc gatcggcgta ttcgcaacgc ggtaacggct agctgatgag gccgtggcgc
     1261 atcgcggtcg ccaccgcggc ggcgcggtcg cccaccgcca gcttcgcgta gatgtgctcg
     1321 aagtgcttct tcacggtcgc agggctcaag gcgagctcct cggcgatccg cggaccggtg
     1381 aggccctcgg ccgcgagctg cagtagctcg acctcgcggc cggtcagcga tgcgacgtgt
     1441 tctggaagct cttgctcatc gccgtcgatg tcgagagcgg tcgccggcgc gaaggcgatc
     1501 acatggaggc ccggcagggc gttcgcaagg ccgtagtaca gcgcctccca gcggctgccg
     1561 tcgggcgact gcacctcgta gggcccggcg acacaacccg cgtcgatcag ccgcgaccac
     1621 gccgcctcca tgagcggcca gctctcgcgt ggggtgagac tgtcgattcg tcgcttgcgc
     1681 atctcggcga ggctcagcct gaaggcgaag cgtgccggct ggttgacctc gacgtagcgc
     1741 cgctcgtcat cgaccatgac catcggcaca ggactgcgcg cgaacacgct cttcagccgc
     1801 cgtccctgtc cacgggcggc gaccaccgcc tcggcagctg cgccaattgg gtaggggacg
     1861 taccggggcc ggacggtcag cgcacctcgc cgtatatgtc agttgactct ttcaaatgcg
     1921 gcaaagcagg gtacatgccg catagtgcgc aaggctgccg agaaaaacgg cattggggct
     1981 cgcgtgcgca gcgcgaggac gcggctgggc tggacgaggg aagcgctcgc gttccactcc
     2041 ggactgagct ggtcggcgat cgcacaggtc gagtcgggcc gccggacgaa cctgcgcccc
     2101 gacacgctct ttgccctcgg aaccgcgctc ggcgtgacga tcgattacct cgttcacggc
     2161 ggtgtgtcga gtcctgtcat gttcaggcac agggcgctgc tctacgggac ggacaggggg
     2221 ttcgcggacg cggtcgtgcc gtgtgaccga gtgcagggcg cgcggcggag gggtcagcc
//
LOCUS       DASVEU010000159        11053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_501393,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000159 DASVEU010000000
VERSION     DASVEU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501393"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VF225_06300"
     CDS             complement(<1..1144)
                     /locus_tag="VF225_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7144903.1"
                     /translation="MVAQANEKRLLVGGEWIETGEWLDVRSPYSGDIVGRVAKAGAGE
                     TRSAIDAAEGAMREPLPAHKRAEILVRVAGALGRRHEEAARLISDEAGKPLKAARVEA
                     ARAMSTYTFAAVEARKLAGEMVPMDAAQAGEGKLAFTLRRPIGIVGAISPFNFPLNLV
                     AHKIAPALAAGCAVVLKPASATPLSALFLAELEEEAGLPPGWLNVLVGPAAEIGDVIV
                     EDERVKLITFTGSSGVGWKLKERAAKKRVNLELGNATPLIVAADADLSTAATKVAQNA
                     FAFAGQSCISVQRVYVERSAYGEFVQQLVPLVEALTVGDPGDEETDVGPVIDEGARER
                     ILEWIKASSGQVLTGGELEGELIRPTVIANPGRDDEVSCEEVFGPVC"
     gene            1164..2309
                     /locus_tag="VF225_06305"
     CDS             1164..2309
                     /locus_tag="VF225_06305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7144904.1"
                     /translation="MLDALIIDAVRSPIGKKNGSLASIRGDELAAQVLNGVVARHDLD
                     PAEIEDVQLGCVTQIGEQGWNIGRMAPLVAGWPESVCGTTVDRQCGSSMQTNFSAATA
                     ISCGQLDLVVSAGVEMMSRVPMGSNGGDLSDKLLDRWQIVPQGISAEVIADEWGFSRE
                     ELDAFSLESHRRATAAADEGRFEGEIVPIDLTNPHTGVVFGVDETPRRDTSAEALAAL
                     KPAFLPEGKVTAGNSSQICDGAAAVLVASAGAAGRLGLEPRARFVSFGLAGVDPHRML
                     HGNPQACERALARAGLAWDDMSVIEVNEAFACVVLQFARDAGLEERWDDINPNGGGIS
                     LGHPLGATGARITATLLNELERRNARYGIATMCIGFGQALACVVERV"
     gene            2335..2982
                     /locus_tag="VF225_06310"
     CDS             2335..2982
                     /locus_tag="VF225_06310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7144905.1"
                     /translation="MAGRAVAREPKEIVARGYDAIALRYAEWAGKVDSPVMEWVRDLA
                     GRLDEGADVLELGCGRGVPATRELARRQRVTGVDISAVQVELARHHVPEASFVHGDAM
                     ELEVAPASFDAVVALFVFGHVPIEEQAGLIARIGLWLRDGGFFLGTFAAGDAGEGIEP
                     DWLGAPMFFASLGGEAYTPLLRDAGLEPVRDEVVVQHEPGHGDVSFRWTLARVAA"
     gene            2982..3830
                     /locus_tag="VF225_06315"
     CDS             2982..3830
                     /locus_tag="VF225_06315"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7144906.1"
                     /translation="MAIRKVGVVGLGTMGAGIAQISVQSGFDTVGREVSDELAERGRG
                     TIERYLARGVEKGRLSEADRDAAVGRLTLTTDLADRADCDLVIEAVLEELELKRELFA
                     ELDRIARPDAILATNTSALSVSEIAEATERPQRVVGMHFFNPAPVLPLVEVVRGRESS
                     DEAVDAAYGWAVQAGKQPVRCNDTPGFIVNRILIPLLNDCVRVLDEAAVTPEEMDKAM
                     TNGAGWPMGPCALIDLIGIDVHVHASEALHGKLDEERMAPPERLVRMQQEGALGRKAG
                     RGFYEY"
     gene            complement(3835..4092)
                     /locus_tag="VF225_06320"
     CDS             complement(3835..4092)
                     /locus_tag="VF225_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144907.1"
                     /translation="MTELTRSERTSRLLVARLDALASVASQISHVEAERLVELASIAT
                     MHAVALETLKAEKAEAIWREAHARHPQLPEVVVELPERLAA"
     gene            4242..4721
                     /locus_tag="VF225_06325"
     CDS             4242..4721
                     /locus_tag="VF225_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144908.1"
                     /translation="MRRLLTFGLLVALALPAASAARTSGATDGTLSVQDARGTITISA
                     RGGVIGSFARGSVTISDPIEGDGTGPIVTGDDFPPVEKNDTTTTWRGTKVRFRIIGGS
                     FRIVVRGRGINLSLVGKGNVTLDGAGTVDDGSYSVNGAEYLPMPEFSLPFPLSSTSP"
     gene            4732..5436
                     /locus_tag="VF225_06330"
     CDS             4732..5436
                     /locus_tag="VF225_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7144909.1"
                     /translation="MSASTQSVLVVEDEASIASFVSLYLKNAGYEVRTASNGTDALAS
                     AQSEQPSLIVLDLMLPDIDGIEICRRIRQKSDVPILMLTARDEDVDKIIGLEVGADDY
                     MTKPFNPRELVARVKSILRRAAPERKEPESAVLQHGDLVIDAGRREVKVGPEEIQLAP
                     KEFDLLWELLDHRGLVLTRDQLLERVWGYTFAGDTRTVDVHVRQLRRKLGEASPIVTV
                     WGVGYKVSPARDAVKA"
     gene            5466..6845
                     /locus_tag="VF225_06335"
     CDS             5466..6845
                     /locus_tag="VF225_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7144910.1"
                     /translation="MFSSLRFRLPALFLLGIVVSGLIASVVALRLFQGYVLNRSKADL
                     RREAVGLTEVFAQQAITANDTGALPRIARQLERATGDRLFYVGVSPVPGKNVGLELLP
                     QSTVDWRSGRTITFEFTPPRANKTYVAVARPLKLEKKGPAFGDLIVAKPKTELTQQLV
                     PLLERLALASLGGILVAGLLGWYLSRRITRPVLALSRATDEIARGSYDVDLPEVRGSD
                     EIGHLADSFRQMATRLSDAERQERNFLMSVSHELRTPLTAIRGHVDALREGVAEDPEA
                     RAASLEVIAREGARLERLVGDVLDLAKLEAHRFTVHTEEVDMERLCDQAYNAFGEEAR
                     RRSIDYAKRFEAQPTILSDGDRVLQIISNLLSNAFRWTPDGGRVQLELSASNGRVSVA
                     IDDNGPGVAFEERERIFRPFWSRDDSGTGLGLAIAHELAAALGGSIALDSRPGEGSRF
                     ELVLPSGPE"
     gene            6858..7766
                     /locus_tag="VF225_06340"
     CDS             6858..7766
                     /locus_tag="VF225_06340"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX7144911.1"
                     /translation="MGLPKTTTVYELPRRRSPLRAALVSLRPRQWTKNLLLFAGIIFA
                     AKLGDLSRWVEAIAAFAAYCAASSASYLVNDVRDAPHDRVHPTKRLRPIAHGELSPRL
                     AEALAAVLVVGAVLLVAPLGLASILFLGTFFALQAAYTLSLKHVVLLDVMTIGGLFVV
                     RAAAGAAAVHVRISPWLLLCTALLALFLALAKRRGELVLVGAEATPGRPVLEGYSLAL
                     VDQLVTVVAASTVISYCLYTFTARDSKAMMITIPFVLFGVFRYLLLMHRRDLGEEPEE
                     ILLRDAPILLCIAGWAVTAAVILSVT"
     gene            complement(7780..8235)
                     /locus_tag="VF225_06345"
     CDS             complement(7780..8235)
                     /locus_tag="VF225_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144912.1"
                     /translation="MDDSVVGRAQERVEQRSEGAALDSLLIRAREQVDSLAAAATALE
                     ETLPACVGEAVRNEAQPVGRNLAEIRGLMNQVLRRLERIEADQLGERNARVDDLALLV
                     DLVSSGWKGVDTRLARMESVISRLEQGMQENRGAVVYRMEDRRPDAASS"
     gene            8263..9195
                     /gene="queA"
                     /locus_tag="VF225_06350"
     CDS             8263..9195
                     /gene="queA"
                     /locus_tag="VF225_06350"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7144913.1"
                     /translation="MKTRELEYDLPPELVAQHPVARRDESRLLVYPRASQRVHERRFA
                     ELPDVIGDALVVVNDTRVVPARIPIEGPKGEVLLLERLDEDGLWEGLARPTRRLRPGR
                     RYGPVELIEHLGEGRWRLRLHGEPAGETPLPPYITEPLADPGRYQTVFARDAGSAAAP
                     TAGLHFTEELLARLDVERVTLHVGLDTFRPVVAEDLGEHQLHGERYAVTPDAWQRISA
                     ASRVLAVGTTTVRVLETIARGGPLSGRTDLFVTPGFDFLRTDALLTNFHLPRSTLLAL
                     VMAFAGVEETRRLYRTAVEQRYRFYSFGDAMLVL"
     gene            9192..9674
                     /locus_tag="VF225_06355"
     CDS             9192..9674
                     /locus_tag="VF225_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7144914.1"
                     /translation="MNVRRAHQEDAVTVAALLDEATEWVGDLGFSQWPLPFPRDQLAA
                     AIDRGEVYVVESEEGEAVATVSMLLDDPIYWGERPPDAFYVHKLAVRRDRAGRGIGAA
                     IVEWANAEAAEAGREFLRVDCLGDNPGIRDYYEDLGFEHRGDLVLDGRNMSLYERSVR
                     "
     gene            9671..10786
                     /gene="tgt"
                     /locus_tag="VF225_06360"
     CDS             9671..10786
                     /gene="tgt"
                     /locus_tag="VF225_06360"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7144915.1"
                     /translation="MSDTYTVTATDGAARAGLLRTTHGEVPTPAFMPVGTKGTVKTVD
                     PEELRALGTPILLGNTYHLHFRPGEDVIAELGGLHRFMGWDGPILTDSGGFQVFSLRD
                     TIVAVDDDGVTFRSVYDGAAARFTPESVAGIQAALGSDIAMCLDICPPADIPRAELAE
                     AVRRTTLWARRQHQAPRAEGQLLFGIAQGASDSELRRRSIAEIAELGFDGHALGGLAI
                     GEERGLMFETTEWAASELPANKPRYFMGIGDPEGVLEVIERGVDMFDCVLPTRTARTG
                     SALTWEGRLNLRNARFARDPRPLDESCRCPACTRFSRAYLRHLINQEELLGLRLLSLH
                     NLRFVLDLTANARAAIEEGRLAAYKSEALGRLASAAA"
     gene            10796..>11053
                     /gene="yajC"
                     /locus_tag="VF225_06365"
     CDS             10796..>11053
                     /gene="yajC"
                     /locus_tag="VF225_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7144916.1"
                     /translation="MSAYIFVLVLLALMWFLIIRPQRRRQQEAQRLLTQIAVGKEIVT
                     AGGLYGTVTAVEGDEVRVEVAEGVEVRIAKRAVAGVVSEDEE"
BASE COUNT         1655 a         3702 c         3819 g         1877 t
ORIGIN      
        1 tgcagaccgg tccgaagacc tcctcgcagg agacctcgtc gtcacgccca gggtttgcga
       61 tcaccgtcgg ccggatcaac tcgccctcca gctcgccgcc ggtcagcacc tgtccgctgc
      121 tcgccttgat ccactcgaga atgcgctcgc gcgcgccctc gtcgatgacg gggccgacgt
      181 ccgtctcctc gtcgcccgga tcgccgaccg tcagcgcttc gacgaggggg acgagttgct
      241 gcacgaactc gccgtacgca ctccgttcca cgtagacccg ctgcacggag atgcagctct
      301 ggcccgcgaa ggcgaacgcg ttctgggcga ccttggtcgc cgcggtcgac agatccgcgt
      361 cggccgcgac gatcagcggc gttgcattgc cgagctcgag gttcacgcgc ttcttcgccg
      421 cgcgctcctt cagcttccag ccgacgccgc tcgagcccgt gaacgtgatc agcttgacgc
      481 gctcgtcctc gacgatcacg tccccgatct cggccgccgg gccgacgagc acgttgagcc
      541 aacccggagg gaggccggcc tcctcttcca gctccgcgag aaacaacgcc gataggggcg
      601 tcgcgcttgc cggcttgagc acgacggcgc agccagcggc cagggccggt gcgatcttgt
      661 gcgccacgag gttgagcggg aagttgaacg ggctgatcgc gccgacgatg ccgatcgggc
      721 ggcgcagcgt gaaggcgagc ttgccttcgc cggcctgcgc ggcgtccatc gggaccatct
      781 ctccggcgag cttgcgagcc tcgactgcgg cgaacgtgta ggtcgacatt gcccgtgccg
      841 cttccacgcg cgcggctttc aggggcttgc ctgcttcgtc cgagatcagc cgcgcggctt
      901 cctcgtggcg gcggccgagc gcaccggcga cgcgcacgag gatctcggcg cgcttgtgcg
      961 ccggcagggg ttcgcgcatc gcgccctcgg cggcgtcgat cgcgctgcgc gtttcgccgg
     1021 cgccggcctt ggccacccgg ccgacgatgt cgccggaata gggtgagcgg acgtcgagcc
     1081 actcgcctgt ctcgatccac tcgccgccga cgagcaaccg cttttcgttt gcctgcgcga
     1141 ccacagagct agtctagacc gggatgcttg acgccctgat catcgacgca gttcgttcgc
     1201 cgatcggcaa gaagaacggt tcgcttgcgt cgatccgtgg cgacgagctc gctgcccagg
     1261 tgctgaacgg cgtggtggcg cggcacgacc tcgatcctgc ggagatcgag gacgttcagc
     1321 ttggttgcgt gacgcagatc ggcgagcagg gctggaacat cggccggatg gcgccgctcg
     1381 tggccggctg gcccgaatcg gtgtgcggaa cgacggtcga ccgccagtgc ggctcgtcca
     1441 tgcagacgaa cttcagtgcc gcgaccgcga tctcatgcgg acagctagac cttgtcgtct
     1501 ccgctggagt ggagatgatg tcgcgcgtcc cgatggggtc gaacgggggc gacctttcgg
     1561 acaagctgct cgaccgctgg cagatcgtgc cgcaaggaat ctccgcggaa gtgatcgccg
     1621 acgaatgggg tttctcgcgc gaggagctcg atgcattctc gctcgagtcg catcgacgag
     1681 ccacggcggc ggccgacgag gggcgcttcg agggcgagat cgttcccatc gatctgacca
     1741 acccgcacac gggtgtcgtc ttcggcgtcg acgagacgcc gcggcgtgac acgtccgccg
     1801 aagcactcgc agcgttgaag cctgcattcc tgccggaggg caaggtgacg gccgggaact
     1861 cgtcccagat ctgcgacgga gccgcagcgg tgctcgtggc cagcgcgggg gccgccggcc
     1921 gcctcgggct ggagccccgg gcgcgcttcg tctccttcgg cctcgcaggc gtcgatccgc
     1981 accggatgct gcacgggaat ccacaggcgt gcgaaagggc gctggcccgc gcaggtctgg
     2041 cctgggacga catgtccgtc atcgaggtga acgaggcctt tgcctgcgtc gtcctgcaat
     2101 tcgcgcgcga cgcgggcctc gaggagcgct gggacgacat caacccgaac ggcggcggca
     2161 tctcgctcgg acacccgctg ggcgcgacag gtgcgcggat cacggcgacc ctgctgaacg
     2221 agctcgaacg ccggaatgcg cgctatggga ttgcgacgat gtgcatcggt ttcgggcagg
     2281 cgctcgcctg cgtcgtcgaa cgcgtctgat cgttactctc cgcgagtggg ggagatggca
     2341 ggacgcgcgg ttgcgcggga acccaaagag atcgtcgctc gcggctatga cgcgatcgcc
     2401 ttgcgctacg ccgaatgggc ggggaaggtc gactcccccg tcatggaatg ggtgcgcgat
     2461 ctcgccgggc ggctcgacga aggcgccgac gtgctcgagc tcggttgcgg ccgcggggtt
     2521 cccgccacac gcgagctcgc tcgtcgccaa cgcgtcacgg gagtggacat ctccgcagtg
     2581 caggtcgagc tcgcgcgcca tcacgtcccc gaggcgagct tcgtccacgg agacgcgatg
     2641 gagctcgagg tcgcgcccgc ctcgttcgac gcggttgtcg cgctcttcgt cttcgggcac
     2701 gttccgatcg aggagcaagc cggcctgatc gcgcgcatcg gcctgtggct tcgcgacggc
     2761 ggcttcttcc tcggcacgtt cgccgcggga gacgccggcg aggggatcga gcccgactgg
     2821 ttgggcgcac cgatgttctt cgcttctctc ggcggcgagg cgtatacgcc ccttctgcgc
     2881 gacgccggtc tggagcctgt gcgtgacgag gtcgtcgtgc agcacgagcc cggccacggt
     2941 gacgtctcct ttcgctggac gctggctcga gtggccgcct agtggcgatc cgaaaagtcg
     3001 gcgtcgtcgg cctcggcacg atgggggcgg ggatcgcgca gatcagcgtc cagtcggggt
     3061 tcgacacggt cgggcgggaa gtgagcgacg agctggccga gcgcggccgc ggcaccatcg
     3121 agcggtatct ggcccggggc gtcgagaaag gccggctttc cgaagcagac cgcgacgctg
     3181 ctgtcggccg cctgacgctg acaacggatc tcgccgaccg tgcggactgc gatctcgtca
     3241 tcgaagcggt gctcgaggag ctcgagctca aacgtgagct gttcgcggag ctcgatcgca
     3301 tcgcccggcc cgatgcgatc ctcgcgacga acacctccgc gctgtccgtt tcggagatcg
     3361 cggaggcgac ggagcggccg cagcgcgtcg tcggcatgca tttcttcaat cccgcccctg
     3421 ttctgccgct ggtcgaggtc gtccgcgggc gcgagagctc ggacgaagcc gtcgacgccg
     3481 cctacggatg ggcagtgcag gccggcaagc agccggtgag gtgtaacgac accccaggat
     3541 tcatcgtcaa ccgcatcctc attccgttgt tgaacgactg cgtgcgtgtg ctcgacgaag
     3601 ctgcggtcac gccggaggag atggacaagg ccatgaccaa cggtgccggc tggccgatgg
     3661 gcccgtgcgc cctcatcgat ttgatcggca tcgacgtgca cgtgcacgcg tcagaggctc
     3721 tgcacggaaa gctcgacgag gaacgcatgg cgccaccgga acgcctggtg cgaatgcagc
     3781 aggagggcgc actcggccgc aaggcggggc gcggcttcta cgagtactag ttggctaggc
     3841 ggcgagtcgc tcgggcagtt caaccacgac ctccggcagc tgcgggtggc gcgcatgcgc
     3901 ctctcgccag atggcctcgg ccttctccgc cttgagcgtc tcgagcgcga cggcgtgcat
     3961 cgtggcgatg gaagctagct ccacgagacg ctcggcttcc acgtggctga tttgcgaagc
     4021 cacgctcgcg agcgcatcga gccgcgccac gagcagtcgc gacgtccgtt cggaacgagt
     4081 cagttcggtc acagctttca tcttcgtggc cgcatcggac ggtcctgctc gcccggttcg
     4141 ggcttaccgt tgtcagctgc cggccgttgc ggtgcccgcc ttacaatctc cttacgttcc
     4201 aatgatgcag ggcacaattg gagcgtcgat actgattcgt gatgaggcgg ctcctcacct
     4261 ttggcctgct cgtagcgctg gctttgcccg cggcctcggc tgcgcgtacc tctggcgcca
     4321 ccgacggcac gctctccgtg caggacgcgc gcggcacgat cacgatctct gcccgaggtg
     4381 gagtcatcgg cagcttcgca cgcggatcgg tcacgatcag cgacccgatc gaaggcgacg
     4441 gcaccggccc gatcgtcacc ggcgacgact ttccgccggt ggagaaaaac gacacgacca
     4501 cgacgtggcg agggacgaag gtgcgcttcc ggatcatcgg tggttcgttc cgaatcgttg
     4561 tcaggggacg cggcatcaac ctctcgctcg tgggcaaggg gaacgtgacg ctggacgggg
     4621 caggcaccgt ggacgacggg tcgtattccg tgaacggtgc ggagtacctc ccgatgccgg
     4681 agttttcgct cccattccct ctctcgtcta ccagtcccta ggggaatccc tttgagcgcc
     4741 agtacgcagt cagtgctcgt cgtcgaggac gaagcgtcga tcgcgtcctt cgtctcgctc
     4801 tacctgaaga acgcgggtta cgaggtccgc accgcgtcga acggcaccga cgcgctcgca
     4861 tccgcgcaga gtgagcagcc gtcgctgatc gtcctcgatc tgatgctgcc ggacatcgac
     4921 ggcatcgaga tctgccggcg catccgccag aagtccgatg tgccgattct gatgctgacc
     4981 gcacgcgacg aggacgtcga caagatcatc ggcctcgagg tcggcgccga cgactacatg
     5041 acgaagccgt tcaatccacg cgagctcgtg gcgcgcgtga agtcgatcct gcgccgcgcc
     5101 gcgcctgagc ggaaggagcc cgagagcgcg gtcctgcaac acggcgacct cgtgatcgac
     5161 gccggccggc gggaggtgaa ggtcggcccc gaggagatac agctcgcacc gaaggagttc
     5221 gacctcctct gggagctgct cgaccatcgc ggccttgtgc tcacgcgtga ccagctgctc
     5281 gagcgagtct ggggctacac ctttgccgga gacacgcgga cggtggacgt tcacgtgcgc
     5341 cagttgcggc gaaagctcgg cgaggcctcg ccgatcgtga ccgtgtgggg ggtcggctac
     5401 aaggtctcgc ccgcgcggga cgcggtcaag gcctagcagg gccgagcagt accttgtagg
     5461 tcctgatgtt cagctcgctg cgcttccggc tgccggcgct cttcctgctc ggcatcgtcg
     5521 tgagcgggct catagcgtcg gtggtggcgc tacggctctt ccagggctac gtcctcaacc
     5581 gctccaaggc cgatcttcgc cgcgaggcgg tcggtctcac ggaggtcttc gcccagcagg
     5641 cgatcacggc caacgacacc ggcgcactgc cgcgcatcgc gagacagctc gaacgcgcaa
     5701 ccggcgatcg cctcttctac gtaggcgtgt cgccggtccc cggcaagaac gtcggactgg
     5761 aactcctacc gcagagcacc gtcgattggc ggtcgggtag gacgattacg ttcgagttca
     5821 caccaccgcg tgcgaacaag acctatgtcg cagttgcgcg tccgctgaag ctggagaaga
     5881 aggggcctgc gttcggggac ctgatcgtcg cgaagccgaa gacggagttg actcagcagc
     5941 tggtaccgct gctcgaacgg ctggcgctcg cctcgctcgg cggcatcctc gtagcggggt
     6001 tgctcggctg gtacctgtcg cgccgaatca ctcggcccgt cctggcgctg tcgcgcgcga
     6061 cggacgagat cgcgcgcgga agctacgacg tcgaccttcc ggaggttcgc ggcagcgatg
     6121 agatcgggca cctagcggac agcttccggc agatggctac gcgcctctcc gatgccgagc
     6181 gtcaggaacg caacttcctg atgagcgtct cgcacgagct gcggacgcct ttgactgcga
     6241 tccgcggtca tgtcgacgct ctccgggagg gagtcgcgga ggatcccgag gcgcgcgcag
     6301 cctccctcga ggtgatcgcg cgggagggcg cgcggctgga gcggctcgtc ggcgacgtcc
     6361 tcgatcttgc gaagctcgaa gcacatcgct tcaccgtgca cacggaggaa gtggacatgg
     6421 agcggctttg cgatcaggcg tacaacgcct tcggcgagga ggcccgccgc cgcagcatcg
     6481 actacgcgaa gcggttcgag gcgcagccca ccatcctctc cgacggcgac cgcgtgttgc
     6541 agatcatctc caacctgctc tcgaacgcgt ttcgctggac cccggacggc gggcgggtgc
     6601 agctcgagct gagcgcgtcg aacggccgcg tgtcggttgc gatcgacgac aacggcccgg
     6661 gcgtcgcgtt cgaggagcgg gagaggatct tccggccgtt ctggtcccgg gacgattcgg
     6721 gcaccgggct cgggctggcg atcgcccacg agctcgctgc cgcgctgggt ggaagcatcg
     6781 cgctcgactc ccgtcccggc gagggcagtc gcttcgagct cgtcctcccg tccgggccgg
     6841 aatagtctcc ggtccgcatg ggccttccga agacgacaac cgtctacgag ctgccacgcc
     6901 ggcgctcgcc gcttcgcgcg gcgctcgtct cgttgcggcc ccggcagtgg acgaagaacc
     6961 ttctcctctt cgcgggaatc atcttcgccg caaagctcgg cgatctcagt cggtgggtcg
     7021 aggcgattgc cgccttcgcc gcgtactgcg cggcctcgag tgcgagctat ctcgtgaacg
     7081 acgtccgcga cgcgccccac gaccgcgtgc atcccaccaa gcgtttgcgg ccgatcgcac
     7141 acggcgagct gtcgccgcgt ctggccgaag cgctcgcggc cgtgttggtc gttggggcag
     7201 tgctgctcgt ggcgccgctg ggcctggcct cgatcctgtt cctcggcacg ttcttcgcgc
     7261 tgcaggcggc gtacacgctc tcgctcaagc acgtggtctt gctggacgtg atgacgatcg
     7321 gcgggctgtt cgtcgtacgt gcagcggcgg gcgcagcggc ggtacacgtg aggatctcgc
     7381 cctggctcct gctctgcacg gcgctgctcg cgctcttcct cgcgctcgcc aaacgccggg
     7441 gcgagctcgt gctcgtgggc gccgaggcca ctcccgggcg gcccgtgttg gagggctatt
     7501 cgcttgcgct cgtcgaccag ctcgtgaccg tcgtggccgc gtcaacggtg atctcgtact
     7561 gcctgtacac gttcacggcg cgggactcga aggcgatgat gatcacgatc ccgttcgtcc
     7621 tcttcggggt gttccgctat ctactcctca tgcaccggcg cgacctcggc gaggaaccgg
     7681 aagagatcct gttgcgcgac gcgccgatcc tgctctgcat cgcaggctgg gccgtcaccg
     7741 cggcggtcat cctctcggtg acctagaggg attgggacgc taggagctgg ctgcgtccgg
     7801 acggcggtct tccatccggt agacgaccgc gccgcggttt tcctgcatgc cttgctccag
     7861 cctgctgatg acactctcca tgcgcgcaag cctcgtgtcg acgcccttcc agccggatga
     7921 gacgaggtcg acgagcaggg cgaggtcgtc gacgcgcgca ttgcgctcgc cgagctggtc
     7981 ggcctcgatg cgttcgagtc gccggaggac ctggttcatc agcccgcgga tctcggcgag
     8041 gttgcgtccg accggctgcg cctcgttgcg cactgcctcg ccgacgcacg ccggcagtgt
     8101 ctcctcgagc gccgtcgctg cggctgcgag cgagtcgacc tgctcgcgtg ctcggatgag
     8161 gaggctgtcg agggcggcgc cttccgaccg ctgctcgacc cgttcctgtg cgcgcccgac
     8221 gacgctgtcg tccatgtccg gcaagggtac cgtgggcacc ggatgaaaac gagggagctc
     8281 gaatatgacc tcccgccgga gcttgttgct cagcatcccg tggcgcgccg cgacgagtcg
     8341 cgactgctgg tctacccacg cgcttcccag agggtgcacg agcgcaggtt cgcagagcta
     8401 ccggacgtga tcggcgacgc gctcgtcgtc gtgaacgaca ctcgggtcgt cccggctcgg
     8461 attccgatcg aagggccgaa gggcgaggtc ctgctgctcg agcgacttga tgaggacggg
     8521 ctctgggaag gcctggcccg cccgacgcgg cgcctgcggc ccggtaggcg ctacggaccg
     8581 gtcgagctga tcgaacacct cggtgaggga cgctggcgtc tccgcctgca cggcgagcct
     8641 gcgggcgaga cgccgctgcc cccatacatc acggagccgc tcgccgaccc ggggcgctac
     8701 cagacggtct ttgcgcgcga cgcgggctcg gccgccgcgc cgacggccgg cttgcacttc
     8761 acggaggagc tgctcgcgcg gctcgacgtc gagcgtgtca cgttgcatgt cgggctcgac
     8821 accttccgtc cggtcgtcgc cgaagacctc ggcgagcacc agctgcacgg cgagcggtac
     8881 gccgtgacgc ccgacgcgtg gcagcgcatc agcgctgcgt ccagagtgtt ggccgtgggg
     8941 acgaccaccg ttcgtgttct cgagacgatc gcgcgcggcg ggcccctctc cggccgcacc
     9001 gatctcttcg tcacgccggg cttcgatttc ctgcgaacgg atgcactgct gacgaacttc
     9061 cacctcccgc gttcgacgct gctcgcgctg gtgatggcct tcgcaggggt ggaggagacg
     9121 cggcggcttt accggacggc ggtcgagcag cggtaccgct tctattcgtt cggcgatgcg
     9181 atgctggtgc tgtgaacgtc cgccgcgcgc accaggaaga cgccgtgacc gtcgccgcgt
     9241 tgctcgacga ggcaacggag tgggtcggtg atcttggctt cagccagtgg ccgctgccgt
     9301 ttccccgcga ccagctcgcc gcagcgatcg accgcggtga ggtctacgtg gtcgagtcgg
     9361 aggaaggtga ggcggtggcg accgtctcga tgctgctcga cgacccgatc tactggggcg
     9421 aacggccgcc ggacgctttc tacgtgcaca agctcgccgt gcggcgcgac cgggccgggc
     9481 gcggcatcgg tgcggcgatc gtcgagtggg ccaacgccga agcggccgag gcggggcgtg
     9541 agttcctgcg ggtcgactgt ctcggcgaca atccgggaat ccgcgattac tacgaggacc
     9601 tcggcttcga gcatcgcggc gacctcgtcc tcgacggccg caacatgagc ctctacgagc
     9661 ggtccgttcg atgagcgaca cgtacaccgt gaccgcgacg gacggcgcgg cgcgggcggg
     9721 actcctccgc accacgcacg gtgaggtccc gacgccggct ttcatgcccg tcgggacgaa
     9781 ggggacggtc aagacggtgg atcccgaaga gctgcgcgcg ctcggcacgc cgatcctcct
     9841 cggcaacacg taccacctgc acttccgtcc gggagaagac gtgatcgccg agctcggagg
     9901 gctgcatcgc ttcatgggct gggacggacc catcctcacc gactccggcg gctttcaggt
     9961 cttctcccta cgcgacacga tcgtcgctgt cgacgatgac ggcgtcacct tccgctccgt
    10021 ctacgacggt gctgccgcgc gcttcactcc cgagtcggtc gcggggattc aggccgcgct
    10081 cggctcggac atcgcaatgt gcctcgacat ctgtccgccc gccgacattc cgcgcgcgga
    10141 gctcgcagag gcagtgcggc ggacgacgct gtgggctcgc cgccagcacc aggccccgcg
    10201 cgccgagggc cagctcttgt tcgggatcgc gcagggcgcg tccgactccg agttgcgccg
    10261 ccgctcgatc gcggagatcg ccgagctcgg cttcgacggt cacgcgctcg gcggcctcgc
    10321 aatcggcgag gagcgaggac tgatgttcga aacgacggag tgggccgcga gcgagcttcc
    10381 ggcgaacaag ccgcggtatt tcatgggcat cggcgatccg gaaggcgtgc tcgaggtgat
    10441 cgagcgcggc gtcgacatgt tcgactgcgt gctaccgacg cggactgccc gcacgggtag
    10501 cgcgctcaca tgggagggtc ggctgaatct ccgcaatgcc cgctttgccc gcgaccctcg
    10561 tccgctggac gagagctgcc gatgtccagc ctgcacccgg ttcagccgcg cctatctgcg
    10621 ccacctgatc aaccaggaag agctgctggg gctacgcctc ctgagcctcc acaatctacg
    10681 attcgtcctt gacctgaccg cgaacgcgcg tgcggcgatc gaggaaggcc ggctggcggc
    10741 gtacaaatcc gaagcgctcg gccggctcgc atcggccgcc gcctgaggag gcctggtgtc
    10801 tgcctacatt ttcgttctcg ttctgctggc cctgatgtgg ttcctgatca tccggccgca
    10861 gcgccgccgt cagcaggagg cgcagaggct gctcacgcag atcgccgtcg ggaaggagat
    10921 cgtcaccgcg ggcggcctct atgggacggt caccgcggtc gagggcgacg aggtgcgggt
    10981 cgaggtcgca gaaggagtcg aggtgaggat cgccaagcgt gcggtggccg gagtagtgag
    11041 cgaggacgag gag
//
LOCUS       DASVEU010000160        12468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_111194,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000160 DASVEU010000000
VERSION     DASVEU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111194"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VF225_06370"
     CDS             complement(<1..1210)
                     /locus_tag="VF225_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7144917.1"
                     /translation="MGMFFHSSDAPPERPADPEVRRANLNRIGRLFAAYRMRLGGVLL
                     LIVVSAGLGVVPAFLLKNVLEAIGRNDTTSLSYNAGGIIAIAIATGVLGVIQTLLSNQ
                     IGQRVMHDLRAAVFRHLQRLSLAFFTRTRTGEVQSRISNDIGGVQNVVTNTATSIASN
                     VTTVAATMVGMLVLNWKLALFAFALIPFFVLLTRRVGNERRRIAKTTQETLADISSLV
                     QESLSVSGILLGKTMGRGDELADRFEADSLRLASLEVRQRMAGRWVMASIQTSFAIMP
                     AAVYWFGGLALAHGSSAISIPMLVAFTTLQTRLFFPVGSLLGVGLDVQTSLALFDRIF
                     EYLDQPIDIEEKADALAVGQAGDVVFDQVWFRYGDDWTLQDVSFTVAAGTTTALVGET
                     GSGKTTLGYLT"
     gene            1259..1579
                     /locus_tag="VF225_06375"
     CDS             1259..1579
                     /locus_tag="VF225_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144918.1"
                     /translation="MDPERARELLARERERIERSLVKRAHTDTGEPAEDNPANLGSDL
                     YQDELDEGLADDLREDLAALERAEARLVAGTYGLSIESGKPIPDERLEALPTAERTAQ
                     EEAH"
     gene            complement(1596..3590)
                     /gene="ftsH"
                     /locus_tag="VF225_06380"
     CDS             complement(1596..3590)
                     /gene="ftsH"
                     /locus_tag="VF225_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7144919.1"
                     /translation="MSPPRSTPTRDQSKNAAAPPSPRGSSPTPPQPPRFVFSRRWIIF
                     ALGLLLLNFYLGSRATQPTSRVRVPYSPFFLEQVTAGHVKEITSKGTAIQGTFTKKEQ
                     YAGSKPTTKFQTEIPAFANNDALSALLQQKRVVVNAEPLDTGAPWWQNLLLGFGPTLL
                     FIFLLFWLMRRAGNVQNVLGSFGRSRARRYQPPGDRVTFEDVAGIDEAKAELTEVVDF
                     LRHPEKYRKLGGRIPHGVLLSGPPGTGKTLLARAVAGEADVPFFSIAASEFVEAIVGV
                     GASRVRDLFVQAKQDAPAIVFIDELDAIGRSRTSGVAGFSGGNDEREQTLNQILTEMD
                     GFDSSTNVIVIGATNRPDVLDQALLRPGRFDRRVAVQPPDRAGREAILKVHTRGVPLG
                     PDVDLGRIASTTPGMVGADLANLVNEAALLAARRSHEVVDESDFTDALERIILGAERQ
                     VMMSLEDRRRTAYHEGGHAIVGMLTEGADPVRKVSIIPRGLALGVTFAAPESDRFNYR
                     EPEIQAKINVALGGRAAEEIVYGETSTGAESDIQQLTEIARQMVGRWGMSSAIGPIAV
                     IPRDGQGAFLPGAAEVSPDTQKLVDTEVRRIVEESHQAVIALLEENRSKLDSLANALL
                     EHETLDEEDAYAAAGVPRTSSTSVADSLAAAAAARSRLAR"
     gene            complement(3618..4502)
                     /locus_tag="VF225_06385"
     CDS             complement(3618..4502)
                     /locus_tag="VF225_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX7144920.1"
                     /translation="MNAPQGGIFALGTASHAYLEFDVSNGRKGRDLVAAISSLREPRT
                     TMGGVNLVSGFRPELWREAAPDGAPAELEGFNEDLIGIEGFVMPATQHDAVLWLSGSA
                     YDVVFDVARNAIAELNGLGSIVEETSSWPYRHDRDLTGFIDGTENPTLVEAPELVLVA
                     EGNPGAGGTILLLQKWAHDAAAWESLSVIEQERVIGRMKLDSVELEDKAPDSHVASTD
                     QDSFGKIFRRNMPYGTVTDHGTMFVGFSSEQRPLSTMLESMAGLTTGVRDALTLYTRA
                     VTGAYYFVPSTDWLGRFA"
     gene            4697..5230
                     /locus_tag="VF225_06390"
     CDS             4697..5230
                     /locus_tag="VF225_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144921.1"
                     /translation="MNDRSHAARATVPGSWMVLTAAALGIFALLTFLFNPMASNAAPA
                     KATVVSTTSTSLGRVLVNSHGHTLYMFGKDKNGKSSCTGQCATFWPPLIASGKLQATA
                     GAKASLLGTTKRTDGRLQVTYKRHPLYTFAKDTAKGQTNGEGINAFGGVWDAVSPAGA
                     KVVKSATGTGTGGGYGP"
     gene            complement(5432..6349)
                     /locus_tag="VF225_06395"
     CDS             complement(5432..6349)
                     /locus_tag="VF225_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7144922.1"
                     /translation="MNLRLRRAEVTEDATGGLLSYTDWARRDIKWLFGSAQALLLVLL
                     VLIGLGPLVWLAKGAVTPTIDTLRNPLALFPHGVAWSNLTDAWSKVNVGRYFWNTIVL
                     AIGSWFCQIVVATTGAFVLSVLRPAWGRVVNALLLGTLFVPVVVLIVPLYIEVVHPPL
                     IHHSFAESYWAIWLPAGASAFNVVLMKRFFDNLPQELFEAARIDGAGPIRLFWSIVLP
                     LSKPILGVVSIFAILAEWKNFLWPFLVLTNSPDRQPLSVRLPAIQAQTQLGVLLASML
                     IAALVPILGFLIFQRSFLRGSGFGGALKG"
     gene            complement(6349..7296)
                     /locus_tag="VF225_06400"
     CDS             complement(6349..7296)
                     /locus_tag="VF225_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7144923.1"
                     /translation="MDNFDVISGAEARRRDRRSPLTWVRGGGLAALAFALPMLVIFAA
                     FSWYPITRLVVLSLQHTNLVDPPTWVGLQNFRDVINDPLFATAVRNTAYFAALALVFG
                     YPIPLIAAVLISESRRFRGIYSALAYLPVVIPPVVGVMLWKYVFYEPSSVGLFNTILG
                     WVHLGPYGWLQSPGTAMPALVVESTWANAGATMIIYLAALTAVDTDLYDAASVDGAGL
                     WHKIWHITLPQLRGVLLITLILQIIGTAQVFLEPYLFTSGGPANATLTVLLLVYQYAF
                     GNSVGVGFGDAAALSLMLAAFLAVFSLVFIRATRSWSTR"
     gene            complement(7416..8801)
                     /locus_tag="VF225_06405"
     CDS             complement(7416..8801)
                     /locus_tag="VF225_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7144924.1"
                     /translation="MKFTRRATTVSVAVAGLLAVCAGLVSSAAAKSGSAGATVTINVA
                     ALRPGSSSDAQKQFDDNVALFEKQHPNIDVKAVEYAWNGATFAAQLAAGRLPTVFTVP
                     FTDARTLGEHRQVADITAGVKKWPYYKKFQKTIIAEATTSKGRIIGVPYAAYAQALHY
                     NRALFSKAGLNPNKPPTTWAQLRKYAKQISDRTGAAGYAQMGLNDNTAGWIATTVTYT
                     LGGRMEQGRGTTATATLNNPYTVQGLNLLRQMRWTDKSMGSTFDYTWPTINQAFAAGK
                     IGMFINGSDIYTFLVQAANLDPKIYGIAPLPLAKNKNAGVLGGGSIAVVRPDTKGAKL
                     DAALKWIDFFYMEKLIKKPAALRDANILVANKQPVGVPAFPIFNKKQYDVANSWIKPY
                     VNVPINQMAPFLKGIFKQNLYPEPAASTQSVYHALDPVVQAVFADRNANIADLLAQAN
                     QVAQKAIREGN"
     gene            complement(8858..10606)
                     /locus_tag="VF225_06410"
     CDS             complement(8858..10606)
                     /locus_tag="VF225_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7144925.1"
                     /translation="MTHPGSTATTPQTPEPDDRAALDGARADARWWRDAVVYQVYVRS
                     FADANGDGVGDLAGVRDRLPYLRDLGVDALWFNPWYPSPLADWGYDIADYRSIDPAFG
                     TLEEAEQLIAEARALGIRTIVDVVPNHVSDQHPWFRAALDSPPGSPERERFWFRPGAG
                     SDGELPPNGWQSIFGGPAWTRTNDAGVPGEWYLHLFAPEQPDLNWTHPDVWAEHEDIL
                     RFWFDRGAAGVRIDSAALLVKDPNLAEETPQHEAGEHPFTDRDALHDIYRRWRSIADG
                     YPEPRVLVGEVWLPDPERLARYLRPDELHTAFNFDFLSCPWEPGPMRASIESALAAHA
                     PVDAPTTWVLSNHDVTRPVTRYGRTDTSFSFESKRDGTPTDLERGTRAARAAALLAMA
                     LPGSMYIYQGEELGLPEVEDIPAERRQDPMWLRSGGVDPGRDGCRIPLPWGGDRPPYE
                     FSPEGAERPWLDQPDDWGRLTVAAQSEAMTSMLNLYRTGLRFRRAAPWSGDGAFRWIP
                     SSDSVLAFARGERFACIVNFGPDPVALPVGAHVLIASNELEGGELPQDTTVWLRQAQD
                     QAPSGDTLPGREHSIG"
     gene            complement(10705..11727)
                     /locus_tag="VF225_06415"
     CDS             complement(10705..11727)
                     /locus_tag="VF225_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7144926.1"
                     /translation="MARKLADVAKQAGVSEATVSRVLNGKPGISDATRAAVLTALDVM
                     GYERPTHLRVRRARMVGLVVPELQNPIFPVLAEVVAGALAQRGFNAVLCTRTGSMSEA
                     EYVEMLLERQVSGMVFAGGQYAEADAPHDHYARLLKLRLPVVLVNAAAEHLDFPRVST
                     DDAVAMEQAYGHLAYLGHERIGLILGPADHVPSRRKLAAFEAIAARATGQALAGELVE
                     RTRFSLEGGHAAATRLIGHGVTGIVCASDPLALGAIRATRRAGLSVPDDISVVGYDDS
                     AFMSCTDPPLTTVRQPIEAIGRAAVEMLVGQIEGAAVSTDELFFEPEIVARGSTAAAR
                     VALAPA"
     gene            complement(11806..>12468)
                     /locus_tag="VF225_06420"
     CDS             complement(11806..>12468)
                     /locus_tag="VF225_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7144927.1"
                     /translation="IATVSHELRTPLTSIHGYLDLLVGDEAGELTDEQRHFLSIAERN
                     TDRLRRLVDDLLLVSELDAGKLRLELGNVDLRALAHESLESARPRAEEGGITLEFLAA
                     SPVRLTGDKLRLGQLLDNVISNALKFTPQGGRVSVRTSRSNGSAVLEVEDTGMGIPAG
                     EQEHLFDRFFRTQAAEQNAIQGSGLGLAISQAIAQAHGGLIEVTSAENAGATFRIVFP
                     AN"
BASE COUNT         2167 a         4065 c         4196 g         2040 t
ORIGIN      
        1 ccgtgaggta gccgagcgtc gtcttgcccg agccggtctc tcccacgagc gcggtcgtcg
       61 tgcctgcggc caccgtgaac gagacgtctt gaagcgtcca gtcgtcgccg tagcggaacc
      121 agacctgatc gaagacgacg tcgccggcct gccccacggc gagcgcgtcc gccttctcct
      181 cgatgtcgat cggctggtcg aggtattcga agatgcggtc gaacaacgca agggaggtct
      241 gcacgtcgag gccgacgccg agcagggacc cgaccggaaa gaagagccgc gtctgcagcg
      301 tcgtgaaggc gacgagcatc ggaatcgaga tcgcggacga tccgtgcgcg agcgcgagcc
      361 cgccgaacca gtagaccgcg gcgggcatga tcgcgaagct cgtctggatc gacgccatca
      421 cccagcggcc tgccatccgc tgacgcacct cgagtgaagc gaggcgcagc gagtcggctt
      481 cgaagcggtc ggcgagctcg tctccgcgcc ccatcgtctt cccgagcagg atccccgaga
      541 ccgagagcga ctcctgcacg aggctcgaga tgtcggcgag cgtctcctgc gtcgtcttgg
      601 caatccgacg cctttcgttg ccgacgcggc gcgtgagcaa cacgaagaag gggatcagcg
      661 cgaacgcaaa cagcgccagt ttccagttca gcaccagcat gcccaccatc gtcgcggcga
      721 cggtcgtcac gttcgacgcg atcgacgtcg ccgtgttcgt gacgacgttc tgcacaccac
      781 cgatgtcgtt cgagattctc gactgcacct cgcccgtacg cgtgcgggtg aagaacgcaa
      841 gcgagaggcg ctgcagatgg cggaagaccg cggcgcgcag gtcgtgcatc acgcgctggc
      901 caatctggtt ggagaggagc gtctggatga ccccgagcac gccggtcgcg atcgcaattg
      961 cgatgatccc gcccgcgttg tacgagagcg acgtcgtgtc gttccgtccg atcgcctcga
     1021 gcacgttttt gagcaggaag gccgggacga cgccgagccc ggccgagacg acgatcagca
     1081 gcaggactcc gccgagccgc atcctgtagg cggcaaagag ccggccgatg cggttcaggt
     1141 tcgcgcgccg cacctccggg tcggcggggc gctccggcgg cgcatcagaa gagtggaaga
     1201 acatgcccat cgagggtcag ggtacgtcgc ggcgcaacat cgaggtaacg tggcacccgt
     1261 ggatcctgag cgggcacgcg agctcctggc ccgagagcgc gagcgaatcg agcggtcgct
     1321 cgtaaagcgc gcgcacacgg acaccggcga gccggccgag gacaatcccg ccaacctcgg
     1381 ctcggacctg tatcaggacg agctcgacga gggtctggcc gacgacctac gcgaggatct
     1441 cgctgcgctc gagcgggccg aggcccgtct cgtcgccggg acctacggcc tctcgatcga
     1501 gagtggcaag ccgattccag acgagcgcct cgaggcactt ccgacagccg aacgcacggc
     1561 ccaagaggaa gctcactgac ggaacgcgtc gggcgttagc gcgcgagtcg cgatcgagcc
     1621 gccgctgctg ccgccaacga gtccgcgacg ctcgtcgagc tcgttcgcgg gacgccggca
     1681 gccgcgtacg catcttcctc gtcgagggtc tcgtgctcga gcagtgcatt cgcaagggaa
     1741 tccagcttgc tgcgattctc ctcgagcaga gcaatgaccg cctggtggga ttcctcgacg
     1801 atgcggcgca cttctgtgtc gacgagcttc tgcgtatccg gcgagacctc ggcggcaccc
     1861 ggcaggaacg cgccctgacc gtcacgaggt atcaccgcaa tcgggccgat cgctgagctc
     1921 atgccccagc gtccgaccat ctggcgggcg atctccgtga gctgctggat gtccgactcg
     1981 gcgccggtgc tcgtctcgcc gtacacgatt tcttctgcgg cgcggccgcc gagagcgacg
     2041 ttgatcttcg cctggatctc tggctcccga taattgaagc ggtcgctctc cggcgccgcg
     2101 aacgtcacgc cgagcgcaag gccgcgtggg atgatcgaga ccttccgcac cgggtcggcg
     2161 ccctcggtga gcatgccgac gatcgcgtga ccaccctcgt gataggcggt gcggcggcga
     2221 tcctcgaggc tcatcatcac ctgccgctcg gctcccaaga tgatccgctc gagcgcgtcg
     2281 gtgaagtctg actcgtcgac gacttcgtgg ctccgtcttg ccgcgagcag cgcagcttcg
     2341 ttgacgagat tcgcgagatc tgccccgacc atgcccggcg tcgtcgacgc gatcctcccg
     2401 aggtcgacgt cgggcccgag aggaacgccg cgcgtgtgca ctttgaggat cgcctctcgg
     2461 ccggcgcgat ccggaggctg caccgcgacc ctgcggtcga atcgaccggg tcggagtagt
     2521 gcctggtcga gcacgtccgg acggttggtc gcaccgatga cgatcacgtt ggtcgacgag
     2581 tcgaagccgt ccatctcggt caggatctga ttcaaggtct gctcgcgttc gtcgttgccg
     2641 ccgctgaagc ccgcgactcc ggacgtgcgc gaccggccga tcgcgtcgag ctcgtcgatg
     2701 aagacgattg cgggcgcgtc ctgcttcgcc tgtacgaaca ggtcacgcac gcgcgacgcg
     2761 cccacgccga cgatggcttc gacgaactcg gacgcggcga tcgagaaaaa cggaacgtcc
     2821 gcctcaccgg cgaccgcccg cgcgagcagc gtcttgccgg tgccgggcgg gcccgagagg
     2881 agcacgccgt gcggtatccg gcctccgagc tttcggtact tctccggatg acgcaggaaa
     2941 tcgaccacct ctgtcagctc cgccttcgct tcgtcgatgc ccgccacgtc ctcgaacgtc
     3001 acccggtcgc cggggggttg gtagcgacgc gcgcgcgacc ggccgaacga tcccagcacg
     3061 ttctgaacgt ttcccgccct tcgcatcagc cagaacagca ggaagatgaa cagaagcgtc
     3121 ggcccgaaac cgagcaggag gttttgccac cacggtgctc cggtgtcgag cggttcggcg
     3181 ttcacgacta cgcgtttctg ctggagcagc gccgacaggg cgtcgttgtt ggcgaacgcg
     3241 gggatctccg tttggaactt cgtggtcggc ttgcttccgg cgtactgctc cttcttcgtg
     3301 aacgtgcctt gaattgcggt gcccttggac gtgatctcct tcacgtggcc tgctgtcacc
     3361 tgctccagga agaacgggct gtacggaacg cggacgcgtg acgtcggctg cgtcgcccgc
     3421 gagcccaggt agaagttcag gagcaggagc ccgagcgcga agatgatcca gcgccggctg
     3481 aagacaaagc ggggcggctg cggcggcgtc ggcgaagatc ctcgcggcga cggcggtgcg
     3541 gcggcgttct tgctctgatc gcgggttggg gtcgatctgg gcggactcac acgtccatct
     3601 tgctcacatc ggccgggcta cgcaaagcgg cccagccagt cggtcgacgg gacgaagtag
     3661 tacgccccgg tgactgcccg tgtgtagagc gtgagcgcgt cgcggacgcc ggtggtcaat
     3721 cccgccatgc tttccagcat cgtggaaagc ggccgctgtt ccgagctgaa gcctacgaac
     3781 atggtgccgt ggtcggtgac cgtgccataa ggcatgttgc gccgaaagat cttgccgaag
     3841 ctgtcctggt cggtgctcgc cacatgggag tccggtgcct tgtcctcgag ctcgacgctg
     3901 tccagcttca tccggccgat cacgcgctcc tgttctatta ctgagagcga ttcccacgcg
     3961 gcggcgtcgt gagcccactt ctgcagcagc aggatcgtcc ctcccgctcc agggtttccc
     4021 tcggccacga ggaccagctc gggggcctcg accagagtgg ggttctcggt accgtcgatg
     4081 aagccggtca gatcgcggtc gtgtcgatag ggccagctcg aggtctcttc gacgatcgag
     4141 cccagcccgt tgagctcggc aatagcgttt cgtgccacgt cgaagaccac gtcgtacgcg
     4201 cttcccgaga gccacagaac ggcgtcgtgc tgcgtggccg gcatcacgaa accctcgatg
     4261 ccgatcaggt cttcgttgaa cccctcgagc tcggcgggcg cgccatcggg ggcagcctct
     4321 cgccagagct ccggcctgaa ccctgagacc aggttgacgc cacccatcgt cgtcctcggc
     4381 tcacgcagcg aagagatcgc cgccaccagg tcgcgtccct tccggccgtt cgagacatcg
     4441 aactccagat aagcgtggga ggcggtgccg agggcgaaga tcccgccttg gggagcgttc
     4501 atgaggcaat cgtgccgcgg tatgcgagcc atgacaacct gggccgagat cacggtcgct
     4561 cccgcagacc cgaacgcgga gttgcctgct agcaggagtc acgttttcgt gttggcaccg
     4621 atgcccggcc agtgccgcca ccgacatctt gtctgggatg gacggcaacc cactagacaa
     4681 ggagtcggat atgcgaatga acgaccgttc ccatgctgcc cgcgcgacgg tgccgggctc
     4741 gtggatggtc ttgacggccg cagcgctcgg aatcttcgcg ttgctcacgt tcctcttcaa
     4801 cccgatggcg agcaacgccg cacccgcaaa ggcgaccgtc gtctcaacga cgtcgacaag
     4861 cctcgggcgg gtcctcgtca actcgcatgg acacacgctc tacatgttcg ggaaggacaa
     4921 gaacggcaag agctcctgca ccggccagtg cgcgacgttc tggccgccgt tgatcgcgtc
     4981 cgggaagcta caagcgacag caggcgccaa ggcgtccctg ctcggaacga cgaagcggac
     5041 ggacggacgg ctgcaggtga cctacaagcg gcacccgctc tacaccttcg cgaaggacac
     5101 ggcgaagggc cagacgaacg gcgagggcat caacgccttc ggcggtgtgt gggatgcggt
     5161 ttcgccggcc ggagcgaagg tcgtgaagag cgcgaccggc acgggaacgg gcggcggcta
     5221 cggcccctag cccactgacg gagggagagg gccgtggcaa gcggccctct catgcgtctg
     5281 ggaagtcact cgccgaggct gctagctcga ccgcggctcc gaaccggagc cgccgtctac
     5341 ctcgatcaga gccgtcggcc cgccgatgtg cgtgatgcgc acgccggtca tgtccctcgc
     5401 caccgcggga tcaccgtccg agccgtgcaa gtcagccctt gagggccccg ccgaatccag
     5461 aaccacgcag gaaggaccgc tggaagatca gaaagcccag gatcggaacg agcgcggcga
     5521 tcagcatcga tgcgaggagg acgcccagtt gcgtctgcgc ctggatggcc ggcagccgca
     5581 ctgacagcgg ctggcgatcc gggctgttcg tgaggaccag gaacggccag aggaagttct
     5641 tccactccgc gaggatcgcg aagatcgaga cgactccgag gatcggcttc gaaagcggca
     5701 acacgatcga ccagaagaga cggatcgggc cggcgccgtc gatgcgcgcg gcctcgaaga
     5761 gctcctgcgg caggttgtcg aagaagcgct tcatcagcac gacgttgaat gcgctcgcgc
     5821 ccgccgggag ccagatcgcc cagtagctct ccgcgaacga atggtggatg agcggtggat
     5881 ggacgacctc gatgtagagg ggaacgatca agacgacgac cggcacgaag agcgtcccga
     5941 gcaggagcgc gttgacgact cgcccccagg cgggccggag cacggagagc acgaatgcgc
     6001 ccgtcgtcgc gacaacgatc tgacagaacc aggacccgat cgcgaggacg atcgtgttcc
     6061 agaaatagcg gccgacgttc accttcgacc acgcatcggt gaggttgctc caggccaccc
     6121 cgtgcgggaa gagggcaagt ggattcctga gcgtgtcgat ggtcggcgtg acggctccct
     6181 tcgcgagcca gaccaacggc cctagcccga tcaggacgag caggacgagc aggagcgcct
     6241 gcgcggagcc gaacagccac ttgatgtcac ggcgcgccca gtccgtgtac gacaggaggc
     6301 cgccggttgc gtcttcggtc acctcggccc gacggagacg aagattcatc acctcgtact
     6361 ccaggaccgg gtcgcccgga tgaacacgag agagaaaacg gccaggaagg ccgcaagcat
     6421 cagactcagc gcagccgcgt caccaaaacc gacgccgacg gagttcccga atgcgtactg
     6481 gtagacgagc agcaagacgg tgagcgtcgc gttcgctggc ccacccgagg tgaagaggta
     6541 gggctcgagg aagacctggg ccgttccgat gatctggaga atcagcgtga tcagcaggac
     6601 gccccgcagc tgaggcagcg tgatgtgcca gatcttgtgc cagaggcccg caccgtccac
     6661 ggacgccgcg tcgtacagat cggtgtccac cgccgtcagt gcggcgaggt agatgatcat
     6721 cgtcgcgccc gcgttcgccc acgtggactc gacgacgagc gccggcatcg cggtgccggg
     6781 ggattgcagc cagccgtacg gtcccagatg gacccagccc agaatcgtgt tgaagaggcc
     6841 gacgctgctc ggctcgtaga agacgtactt ccagagcatc acgccgacga ccggcgggat
     6901 gacgacgggt aggtaggcaa gcgcgctgta gatcccgcgg aagcggcgcg actcgctgat
     6961 cagcactgcg gcgatgagcg ggatcggata accgaagacc agggcgaggg ccgcgaagta
     7021 ggcggtgttc ctgacagccg tcgcgaaaag cgggtcgttg atcacgtccc ggaagttctg
     7081 cagacccacc cacgtcggcg gatcgacgag gttcgtgtgc tgtagcgaga ggaccacgag
     7141 cctcgtgatc ggataccacg agaacgccgc gaagatcacg agcatgggca gggcgaaagc
     7201 aagagcggct aggccgccgc cgcgtaccca cgtgagaggt gagcggcggt cgcggcgacg
     7261 cgcctcggca cctgagatca cgtcaaagtt gtccacggca cggtcccgag ctctcaccct
     7321 cgccgcgctc accttacgcc gcccgggcag ccgcgtcgcc ggaccagccg acggggagcc
     7381 acgagggctc cccgtcgagc cagcccgtca agaagctagt tcccttccct gatggccttc
     7441 tgggcgactt ggttcgcctg cgcaagcagg tccgcgatgt tggcgttcct gtccgcaaac
     7501 accgcctgaa cgaccggatc gagcgcgtgg tacacgctct gcgtcgaggc agcaggctcc
     7561 gggtagaggt tctgcttgaa gatgcccttc aggaacggcg ccatctggtt gatcggaacg
     7621 ttgacgtagg gcttgatcca ggagttggcg acgtcgtact gcttcttgtt gaagatcggg
     7681 aacgccggaa cgccaacagg ctgcttgttc gcgacgagaa tattcgcgtc tcggagcgcc
     7741 gccggtttct tgatcagctt ctccatgtag aagaagtcga tccacttcag agcggcatcc
     7801 agcttcgcac ccttcgtgtc aggccgtacg accgcgatcg atccgccgcc cagcacgccg
     7861 gcgttcttgt tcttggccag cggcagcggc gctattccgt agatcttcgg atcgaggttc
     7921 gcggcctgga cgaggaacgt gtagatgtcc gagccgttga tgaacatgcc gatcttgccc
     7981 gccgcgaatg cctgattgat cgtcggccag gtgtaatcga acgtcgagcc catcgatttg
     8041 tcggtccagc gcatctgccg gagcaggttc aggccctgga cggtgtacgg gttgttgagc
     8101 gttgcggtcg cggtcgtccc gcggccctgt tccatccggc cgccgagcgt gtaggtcaca
     8161 gtggtcgcga tccagccggc ggtgttgtcg ttgagaccca tctgcgcgta cccggccgcc
     8221 ccggtccggt ccgagatctg tttcgcatat ttccggagct gcgcccacgt cgtcggcggc
     8281 ttgtttggat tgaggcccgc cttgctgaag agcgcacggt tgtagtggag cgcctgcgca
     8341 tacgcggcgt agggaacgcc gatgatgcgg cccttggacg tcgtcgcctc cgcgatgatc
     8401 gtcttctgga acttcttgta gtagggccac ttcttcacac cggcggtgat gtcggcgacc
     8461 tgacggtgtt caccgagcgt gcgcgcgtct gtgaacggga cggtgaaaac cgtcggcaga
     8521 cgccctgcgg ccagctgcgc cgcgaaggtg gctccgttcc acgcgtactc gaccgctttg
     8581 acatcgatgt tcggatgttg cttctcaaac aacgccacgt tgtcgtcgaa ctgtttctgt
     8641 gcgtcgctcg aactaccggg acgcagcgct gcgacgttga tcgtgaccgt cgcaccggcg
     8701 gacccgctct tcgcggccgc gctcgagacc aagccggcgc acacagccag caggcccgcc
     8761 acggcgacgg aaacggtcgt ggctctacgg gtgaacttca tggtcctact cctttcttgc
     8821 gcaccatgtc gctgagccgg tgcgggtccg tgttgaccta gccaatgctg tgttctcgtc
     8881 cgggcaatgt gtccccggac ggagcttgat cctgggcctg gcggagccag accgtggtgt
     8941 cctgcgggag ctcacctcct tcgagctcgt tgctggcgat gaggacgtgg gcgccaaccg
     9001 gcagcgcgac cgggtcgggc ccgaagttga cgatgcaggc gaagcgctcg ccacgtgcga
     9061 aggcgaggac cgagtcgctc gaaggaatcc accggaaggc cccgtcacca gaccacggtg
     9121 cggcgcggcg gaaacgcagc ccggtgcggt agaggttgag catcgaagtc atggcctctg
     9181 actgcgctgc gaccgtgagt cgcccccagt cgtcgggttg gtcgagccag ggccgctcgg
     9241 ctccctccgg actgaactcg tacgggggcc ggtcgccacc ccagggcaga gggatgcggc
     9301 agccgtcgcg acccgggtcg acgccgcccg aacggagcca catcgggtcc tgacgacgct
     9361 cggccgggat gtcttcaacc tccggcaagc cgagctcctc gccctggtag atgtacatcg
     9421 agccgggcaa cgccatcgcg agcagcgctg ctgctcgcgc cgcccgagtg ccgcgctcca
     9481 ggtccgtcgg ggtcccgtct cgcttggact cgaacgagaa ggacgtgtcg gtccggccgt
     9541 agcgggtgac gggcctcgtc acatcgtggt tcgacagcac ccaggtcgtc ggcgcgtcga
     9601 caggagcgtg cgccgcgagt gcggactcga tcgacgcgcg cattggtccc ggctcccacg
     9661 ggcacgagag gaagtcgaag ttgaaggcgg tgtgcagctc gtccgggcgg aggtagcggg
     9721 cgagccgctc cgggtcgggt aaccagacct cacccacgag gacgcgcggc tcggggtagc
     9781 cgtcggcgat cgagcgccag cgccggtaga tgtcgtggag cgcgtcacgg tccgtgaacg
     9841 ggtgctcgcc ggcttcgtgc tgcggtgtct cctcggcaag gttcgggtcc ttgacgagca
     9901 acgccgccga gtcgatccgc acgcccgccg cgccgcgatc gaaccagaac cggaggatgt
     9961 cctcgtgctc ggcccagaca tcggggtgtg tccagttgag gtccggctgc tcgggcgcga
    10021 acaggtggag gtaccactcg ccgggcacgc cggcgtcgtt ggtgcgggtc cacgctgggc
    10081 cgccgaagat cgactgccaa ccgttcggcg ggagctctcc gtcggagccg gcgccgggtc
    10141 ggaaccagaa acgctcccgc tcgggcgagc cgggaggtga gtccagggca gcgcgaaacc
    10201 atgggtgctg gtcggagacg tggttcggga cgacgtcgac gatcgtgcga atcccgagcg
    10261 cccgggcttc ggcgatcagc tgctcggcct cctccagtgt cccgaaggcc ggatcgatcg
    10321 agcgatagtc cgcgatgtcg tagccccaat ccgccagcgg agacgggtac caggggttga
    10381 accagagggc gtcgaccccg aggtcgcgca ggtagggcag ccgatcccgc acgcccgcga
    10441 ggtctcccac tccgtcgccg ttcgcgtcgg cgaagctgcg gacgtacacc tggtagacga
    10501 cggcgtcacg ccaccaccgc gcatcagcgc gagccccgtc cagtgcggcc cggtcgtcgg
    10561 gctcgggtgt ctgcggcgtc gttgccgtcg atcccggatg tgtcattcgc gtcgaggatc
    10621 gcacattcga caatcgaatg caagagtcgg acaagaactt gaatttttcc tactttgcgg
    10681 tccaccaccg caatttcttg cggctcaggc gggggcgagc gcgacgcgtg ctgcggcggt
    10741 cgagccgcgg gcgacgatct ccggctcgaa gaacaactcg tccgtggaga ccgccgcacc
    10801 ctcgatctgt cccaccagca tctcaaccgc ggcgcgcccg atcgcctcga tcggctggcg
    10861 aaccgttgtc agcggaggat ccgtgcagct catgaacgcg gagtcgtcgt accccacgac
    10921 cgagatgtcg tccggaaccg acagcccggc gcgccgtgtc gcgcggatgg caccgagcgc
    10981 cagcggatcg ctggcgcaca cgattccggt cactccgtgt ccgatgagcc gggttgcggc
    11041 ggcgtgcccg ccctccaggg agaatcgcgt gcgctcgacg agctcgccgg cgagcgcctg
    11101 tccggttgcg cgagccgcaa ttgcctcgaa cgcggccagc ttgcgacgcg acggaacatg
    11161 gtcggccggc ccgaggatga gcccgatccg ttcatggccc aggtaggcga gatgaccgta
    11221 ggcctgctcc atcgcgacgg cgtcgtcggt cgacacccgc gggaagtcga ggtgctccgc
    11281 cgcggcgttg acgaggacga ccggcagccg cagcttcagc aggcgcgcat agtgatcgtg
    11341 cggcgcatcg gcctcggcgt actgcccgcc ggcgaacacc atcccggaca cctgtcgctc
    11401 caggagcatc tcgacgtact cggcttcaga catgctcccg gtccgcgtgc acaagacggc
    11461 gttgaaccca cgctgagcaa gcgcgccggc aaccacttcc gcaagaaccg gaaagatcgg
    11521 gttctgcagc tcgggcacga cgagcccgac catgcgcgca cgccgcaccc gcagatgcgt
    11581 cgggcgctca tagcccatca catcgagagc ggtgagaacc gcggcacgag tggcatcgga
    11641 gatcccaggc ttgccgttga gaacccggct caccgtcgcc tcactgacgc cggcctgctt
    11701 cgccacgtct gcgagcttgc gcgccacgct gcaactatac gacaaatcag tgcaaatcct
    11761 gcctccgccc gacgtcgagc ctcactttcg tgagagcctg ccgaattaat tcgccggaaa
    11821 gacgatccgg aacgtggcgc cggcgttctc cgcgctggtg acttcgatca gcccgccgtg
    11881 agcctgtgcg atggcctgcg agatcgcgag gccgaggccg cttccttgaa tcgcgttctg
    11941 ttccgcggct tgcgtgcgga agaaacggtc gaacaggtgc tcctgctcgc cggcggggat
    12001 gcccatccct gtgtcttcca cctcgagcac ggctgagccg ttcgaccggc tcgtgcgcac
    12061 ggacaccctt cctccttgcg gagtgaactt gagcgcgttc gaaatcacgt tgtccagtag
    12121 ttggccgagc cgcagcttgt cgccggtcag tcgaacaggg ctcgcagcca ggaactcgag
    12181 tgtgatgcca ccctcctctg cgcgcggacg tgcggattcg aggctctcgt gggcgagagc
    12241 gcgcaggtcg acgttgccta gctcgagcct gagcttgccg gcgtcgagct cggacacgag
    12301 gagcaggtca tcgacgaggc gtcgcagacg gtcggtgttg cgctcggcaa tcgagaggaa
    12361 atgacgctgt tcgtcggtga gctctccggc ttcgtccccg acgaggagat cgaggtagcc
    12421 gtggatcgat gtcaggggag tgcggagctc gtgcgagacg gttgcgat
//
LOCUS       DASVEU010000161         7733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1470904,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000161 DASVEU010000000
VERSION     DASVEU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470904"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2461
                     /locus_tag="VF225_06425"
     CDS             <1..2461
                     /locus_tag="VF225_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7144928.1"
                     /translation="LSLGRPLVVSDLGWFSELPDDVALKVPVDEDEIPSLATALELLA
                     SSEATQLAMSDAARAYVRREHELGRVAENYAAALEEAAGGTTVADAVVAEIAQAAAEI
                     GIEPGTPFAAELAGRLDDVGLARNGRPEPAPPPQRSPLARVPVWAWLAGLVVFSAVFR
                     YGLSRRVVAPWIMVDELIYSELAKSFAQTGHFLIRDMHHGAYGVVYPVLIAPAWRVFG
                     SVQDAYAAAKTIGSVLMSLTAIPVYFLARRVMTSIPSLLAALLAVAVPSLMYTGTLMT
                     ETVFYPLFACVALALVLALERPTIQRQLVLLGLCALAFLTRSQAIVLLPAVATAPLLL
                     AWLDRRRLRMLADFRVLYAALAAAVLAVLVVQLARGHSPYDVLGSYSVTGHTTYRPDQ
                     VLKWIIFHVAELDLYVGIAPFAALLLLTFVARSLDRPLRVFLAAALPLTAWLLLEVGA
                     FASALSPRIQERNLFYVAPLFLIALLAWIERGMPRPPRAAATAAVLAAALPGAIPYHR
                     LIDTSAESDTLALLPLWWLQQTIVGLDTIPVVVVAAAAAIGLLFFSVSARYALVLPAV
                     VLLWFASATERIERFDHGFPKASVGALYQGITVPQRDWIDAAVGRDADVAFVYSGKDP
                     THHPDTLWENEFFNRSVGPVYDLRQPSMGGLPETQVSQRTDGVLLADGKPVRHAYVLS
                     EESVPLAGTVVARDEGRGMALRRTDGLVRIGYRVRGLYPNDTWSGKHVTYTRLRCAGG
                     AVTAQLSRDPNLVSRPQTVRAEGRSVTFGSNDVAMLTVPLQPRDGVCVATFTVSPTAV
                     PGPADPRVLGVHFLEFHYAAP"
     gene            2458..3483
                     /locus_tag="VF225_06430"
     CDS             2458..3483
                     /locus_tag="VF225_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7144929.1"
                     /translation="MRVAYDVTPLSHPRTGVGNYILGALRGMVEAADGSQELVVFGPV
                     SIRGRKLLDDALADLPVERQIVTVPFGHAVRTAWSRLGRPRAERFLGHTDVLHFTDWM
                     VPPGRSRVRATMIHDLGPLLYPDRLHPRTVRMHTATARAAASCDVVFTNAEYTANDVV
                     ERLRIPRERIRIAYPGVDPRYRPDGARYESVGPYVFTTATEDWRKNRGVVEAALRLRG
                     DDLALLSLGDGGRGYVADEQLPALYRGAEVFAYPSRFEGFGIPIIEAMACGVPCVVSS
                     HPSLDEACGDAAVRADPESPEAFADAIGRALAKREELVSRGLAHARRFDWLETGRIHL
                     RGYADAL"
     gene            3483..4463
                     /locus_tag="VF225_06435"
     CDS             3483..4463
                     /locus_tag="VF225_06435"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7144930.1"
                     /translation="MRVAIDVSPLAQTRAGTARYLKGLMRELERRDDVAVSTRSFGGK
                     SRSSTLARDGIWYPFLLGRERAADVLHCPTYRGPLRSSVPLVVTVHDLAVLRHPEAFN
                     RWTRAYSPRVVPGVLRAAQRVIAVSEFTRRELVELLGVPDEKIRVVPNAVGDEFTREG
                     PAADGDYVLAVGTLEPRKNLARLVEAVRRTDRELRVVGARGWGGVEVRGNGVRWLGEV
                     DDAELARLYRGATCVAYPSLYEGFGIPILEAMACGAPVVTTRGTAMEEVADGAAVLVD
                     ARDPAEIAAGIERAAAEHDALVARGLERARAFRWDAVAAATVEIYREAAQ"
     gene            4460..5470
                     /locus_tag="VF225_06440"
     CDS             4460..5470
                     /locus_tag="VF225_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7144931.1"
                     /translation="MSDRPLVLVDADVLGRHRTGDETYVTQLLRELPAVAEDLRIVAV
                     TRDPELVPDGIEAVQLPARSQELRMAVALPGLLRRLRPDVAHFIHALPLRCPCPAVVT
                     VQDLSWERDPSVFGFWDLAIFKVFVRRAVRKAERVLAISERTKRDLIELYGTAPDKVV
                     VTPLAPDPAFVQAGDHDSFLLFVSAIEPRKRPLAAIDAANAVGRKLVVVGPPKDPELA
                     AELTRRGADVRGYVPKDELVRLYQQAACLVFPSRYEGFGLPVVEAMACGTPVVAAPEE
                     AMQEVAGDAAIFTADLADGVRRALADRARLSALGLERAKTFSWRETARITADVYRSVL
                     AA"
     gene            5482..6237
                     /locus_tag="VF225_06445"
     CDS             5482..6237
                     /locus_tag="VF225_06445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7144932.1"
                     /translation="MFVATGPGEAELLRTSLPALRPQVDEIVVVANGPSSRPGDFSRD
                     ALVVENDRALGFSANVNKGIAATSAEYVVIANPDAVPERDCVSELVAFADAHPRCGIA
                     GPKMINPDGTLQASRRRFPTVGGTIVRRTPLRSLFPPLRWQRRHYLLDAPEDEPLQVD
                     TMLGAFLLMRRSMLDEIGGWDAGYKLYVEDIDLSYRAMKAGWERWWVPSAVVHHEYQA
                     VIDKHFLTRRNLWHLHAMLRFLRKYPERLFAIR"
     gene            6234..6854
                     /locus_tag="VF225_06450"
     CDS             6234..6854
                     /locus_tag="VF225_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7144933.1"
                     /translation="MTGGKYDAQAERWSETAYADPATYLAHRARLVFSVGPRLEPGDT
                     VLDLACGDGGLADHLQAVRYSGVDASPEMVRAATQRGRNVVLGDLNDYEPPHPVAVTT
                     CFRAIYYAHDRPGFFRRVAAYTERKFVFDLNPRQYRVEDVRADLRFAGFDQLELRPFF
                     SSQRFALPRPLAGALHFLERSGPLAGLLLAVRFSYFCAAFRGGKNT"
     gene            complement(6815..>7733)
                     /locus_tag="VF225_06455"
     CDS             complement(6815..>7733)
                     /locus_tag="VF225_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144934.1"
                     /translation="LLLAPRVLRRRAAWVAVAVAAAVIGWNLTGEFAFASASNRFSDR
                     FGENIRPPFTWVDDVTRGAPTLYIGQQMADQNGEWLTEFWNRSIKAVWSLDGTAQGPG
                     PFLTPDPRPSDGALCSHGRCGNWGYPYVVEEQGIDVVGKTFAIHLHRAGGGLEKWRLV
                     KIEPPLRLGGAVTDVYPDGWMGSFSSYTRYATEDNRAGRMRVLLSGPPQGPHATTGHV
                     TVAIGPIVVGDDNQPHLGKPTEVRRFDLRSKGQRVLTFHAPGPRFRVEVTVKPTFVPK
                     ELSADQSDNRHLGAQVRYVFLPPRKAAQK"
BASE COUNT         1090 a         2655 c         2610 g         1378 t
ORIGIN      
        1 gctctcgctc ggacggccgc tcgtcgtcag cgatctcgga tggttctccg agctgccgga
       61 cgacgtcgcg ttgaaggtgc ccgtggacga ggacgagatc ccgtcgctcg cgacagccct
      121 ggagctgctc gcctcgagcg aggccacgca gctcgccatg agcgacgcgg cgcgcgcata
      181 cgtgcggcgt gagcacgaac tcgggcgcgt cgcggagaac tatgcggccg cactcgaaga
      241 agccgcaggg ggaaccactg tcgcggacgc ggtcgtggcc gaaattgcgc aggccgccgc
      301 cgagatcggc atcgagcccg ggacaccctt cgcggcggag ctcgcaggcc ggctcgacga
      361 cgtcgggctg gcccgcaacg gccggccaga gccggcgccc ccgccgcagc gcagccctct
      421 tgcccgcgtt ccggtctggg cgtggctggc ggggcttgtc gtcttctccg cggtctttcg
      481 ctacggcctg tcgcgccgcg tcgttgctcc gtggatcatg gtcgacgagc tcatctactc
      541 ggagctcgcc aagagcttcg cgcagacggg ccatttcctg atccgtgaca tgcaccacgg
      601 cgcgtacggc gtcgtgtatc cggtgttgat cgcaccggcc tggcgtgtct tcggttcggt
      661 gcaggacgca tacgccgcgg ccaagacgat cggctcggtg ctgatgtcgt tgaccgcgat
      721 cccggtctac ttcctcgcgc gccgggtcat gacgtcgatc ccctcgctgc tcgcagcgtt
      781 gctggccgtc gccgtgccgt cgctgatgta cacggggacg ctgatgaccg agaccgtctt
      841 ctatccgctg ttcgcatgcg tggcgcttgc cctcgtgctc gcgctcgagc gccccacgat
      901 ccagcggcaa ctggtgctcc tcggactttg cgcgctcgcg ttcctgaccc gctcgcaggc
      961 gatcgtgctc ctgcccgcag ttgcgacggc accgctgctg ctcgcgtggc tggaccgacg
     1021 ccgcctgcgc atgctcgccg acttccgcgt tctctacgcg gcgctcgctg cggccgtgct
     1081 ggccgtgctg gtcgtccagc ttgcccgcgg ccattccccg tacgacgtat tgggcagcta
     1141 cagcgtgacc ggtcacacga cctaccggcc agatcaggtg ctcaagtgga tcatcttcca
     1201 cgtcgccgag ctcgacctct acgtcggcat cgccccattt gcggcgctcc tgctcctgac
     1261 gttcgtcgcc cgttcgctcg accgaccgct ccgcgtcttt ctcgccgcgg cgctgcccct
     1321 gacggcctgg ttgttgctcg aggtcggcgc cttcgcatcg gcgctttcgc ctcgcatcca
     1381 ggagcgcaac ctgttttacg tcgcgcccct ctttctgatc gctctcctgg cgtggatcga
     1441 gcggggaatg ccgcgacctc ctcgcgccgc cgcgactgcg gccgtgctcg cggccgcgct
     1501 gcccggagcg attccatatc accgcctcat cgacacctcg gcagagtcgg acaccctcgc
     1561 cttgctgccg ctctggtggc ttcagcagac gatcgtcggg ctcgacacga tcccggtcgt
     1621 ggtcgtcgca gcggctgctg cgatcggcct cctcttcttc tccgtctcgg cgcgttacgc
     1681 gctcgtccta cctgcggtag tgctgctctg gttcgcgtct gcgacggagc gaatcgagcg
     1741 cttcgatcac ggcttcccga aggcgtccgt cggtgcgttg taccagggca tcaccgtgcc
     1801 gcagcgcgac tggatcgacg ccgcggtcgg ccgcgatgcc gacgtggcgt tcgtctactc
     1861 cggcaaggat ccgacacacc atcccgacac gctgtgggag aacgagttct tcaaccgcag
     1921 cgtcgggccg gtgtacgacc tacgccagcc gtcgatgggc gggctgcccg agacccaagt
     1981 gtcccaacga acggacggcg tgctgctggc cgacgggaag ccggtgcgtc acgcctacgt
     2041 cctgagcgag gagagcgttc ctctcgccgg cactgtcgtt gcgcgtgacg agggcagggg
     2101 gatggcgcta cggcggaccg acgggctcgt gcggatcggc tatcgcgtgc gcggcctcta
     2161 tcccaacgac acctggtcag gtaaacacgt cacgtacaca cgccttcgct gcgcgggcgg
     2221 tgcggtcacg gcgcagttga gccgcgaccc gaatctcgtc agccgtccac agaccgtgcg
     2281 ggcagagggc aggagcgtga cgttcggctc caacgacgtc gcgatgctga ccgtcccgct
     2341 gcaaccgcgc gacggggtct gcgtcgcgac cttcacggtg agtcccactg ccgtccccgg
     2401 gcccgcggat ccccgcgtgc tcggtgtgca cttcctcgag ttccactacg ccgctccgtg
     2461 agggtcgcgt atgacgtcac gccgctctcg catccgcgga cgggcgtggg caactacatc
     2521 ctcggcgctc tgagagggat ggtggaggcg gcggacggat cccaggaact ggtcgtcttc
     2581 ggtccggtca gcatccgcgg gcggaagctg ctcgatgacg cgctggcgga tcttccggtc
     2641 gagcggcaga tcgtgacggt gccgttcggg cacgcggttc gcacggcctg gagccggctg
     2701 ggacgtccgc gcgcagaacg cttcctcggt cacacagacg tgctgcattt cactgactgg
     2761 atggtcccgc cggggcgctc acgcgtccgc gccaccatga tccacgatct gggcccgctg
     2821 ctctatcccg atcggcttca tccgcgcacg gtgcgcatgc acacagcgac tgcccgtgcg
     2881 gcagcgagct gcgatgtcgt cttcacgaac gcggagtaca cggcaaacga tgtcgtcgaa
     2941 cgtctgcgga ttccgcgcga gcggatccgc atcgcgtatc ccggcgttga ccctcgctac
     3001 cgacccgatg gtgcccgata cgaaagcgtc ggtccgtacg tcttcacgac cgcgaccgag
     3061 gactggcgca agaaccgcgg tgtcgtcgag gccgcgctga gactacgcgg cgacgacctc
     3121 gcgctgctgt cgctgggcga cgggggccgc gggtacgtcg ccgacgagca actgcctgcc
     3181 ctctatcgcg gcgcggaggt cttcgcgtac ccgtcgcggt tcgagggttt tgggattccg
     3241 atcatcgagg cgatggcatg cggggttccc tgtgtggtct cctcgcaccc ttcgctggac
     3301 gaggcctgcg gggatgccgc agttcgcgcg gatccggaga gccccgaggc gttcgcggac
     3361 gcgatcggcc gcgcgctcgc caaacgtgaa gagctcgtct cacgggggct ggcccacgca
     3421 aggcgcttcg attggctcga aacgggccgg attcacctcc ggggttacgc tgacgcgctg
     3481 tgatgcgcgt cgcgatcgac gtctccccgc tcgctcagac ccgtgcggga acggcgcgtt
     3541 atctgaaagg gctgatgcgc gagctcgagc ggcgcgacga cgtggcggtc tccactcgct
     3601 cgttcggagg gaagagtcgt tcgtcgacgt tggcgcgcga cgggatctgg tacccgttcc
     3661 tgctcggacg ggaacgcgcc gccgacgtcc tgcattgtcc gacgtaccgc ggccctctgc
     3721 gctcgagcgt tccgctggtc gtcaccgtgc acgacctcgc cgtgctccgg cacccggagg
     3781 cgttcaaccg ctggacgcgc gcctacagtc cgcgcgtcgt gccgggggtc cttcgcgccg
     3841 cgcaacgggt gatcgcggtg tccgagttca cgcggcgtga gctcgtcgag ctgctcggag
     3901 tgccggacga gaagattcgt gtcgttccga acgccgtcgg cgacgaattc acccgcgagg
     3961 gcccagccgc ggacggggac tacgttcttg ccgtcggaac gctcgaaccg cggaagaatc
     4021 tcgcccggct cgtggaggcc gtccgccgca cagaccgtga gctgcgtgtc gtcggagcgc
     4081 gcggctgggg tggggtcgaa gtccgcggga acggtgttcg ctggctcggg gaggtcgacg
     4141 acgcagagct cgcgcgtctc taccgcggag cgacttgtgt tgcgtacccg tcgctctatg
     4201 aaggcttcgg aattcccatc ctggaggcga tggcctgcgg cgcgccggtg gtcaccaccc
     4261 ggggcactgc gatggaggag gtggcggacg gtgccgccgt cctcgtcgat gcgcgcgatc
     4321 ccgctgagat cgccgccggg atcgaacgcg ccgccgcgga gcatgacgca ctcgtggcgc
     4381 gtgggctcga gcgcgcccgc gcctttcgct gggatgccgt agctgcggcg accgtcgaga
     4441 tctaccgcga ggctgcccag tgagcgatcg accgctcgtg ctcgtcgacg ccgacgtgct
     4501 cggacgtcac cgaaccggcg acgagaccta cgtcacgcag ttgctccgcg agctcccggc
     4561 tgtcgcggaa gatcttcgca tcgtcgccgt gacgcgagac ccggagctcg tcccggacgg
     4621 gatcgaggcg gtgcagcttc ccgctcgatc gcaggagttg cgcatggcgg ttgcgcttcc
     4681 aggtctgttg cgtcggctcc gccccgacgt cgcgcacttc atccatgcgc tgccgctgcg
     4741 ttgcccttgt cctgcggtcg tcacggtgca ggacctctcc tgggagagag atccaagtgt
     4801 cttcggtttc tgggatctcg caatcttcaa ggtcttcgtg cgtcgcgcgg tgcggaaggc
     4861 cgagcgcgtg ctcgcgatct ccgagcggac gaagcgcgat ctgatcgagc tgtacgggac
     4921 ggcgccggac aaggtcgtcg tcacgccgct cgccccagat ccggcgttcg tgcaggcggg
     4981 cgaccacgat tcgttcctcc tcttcgtcag cgcgatcgag ccgcgaaagc ggccgctggc
     5041 cgcgatcgat gcagcgaacg cggtcggtcg caagctggtc gtggttgggc cgccgaagga
     5101 tccggagctc gccgccgagt tgacgcgtcg aggggcggac gtgcgcgggt acgtccccaa
     5161 ggacgagctg gttcggctat accagcaggc cgcgtgcctg gtcttccctt cgcgctatga
     5221 aggcttcggt ctgccggtgg tcgaggcgat ggcctgcggg acgcccgtcg ttgccgcgcc
     5281 tgaagaggcg atgcaggaag tcgccggcga cgcggcgatc ttcacggctg accttgccga
     5341 cggcgtgcgc cgtgcgctcg cggaccgggc gcgactctcc gccctgggcc tcgaacgggc
     5401 caagacgttc tcctggcgcg agacggcgcg gatcactgcc gacgtctacc gaagcgttct
     5461 cgccgcatga aggtttcggg cgtgttcgtg gccaccgggc ctggtgaggc cgagctgctg
     5521 cgcacttcgc tccctgcgct gcggcctcag gtcgatgaga tcgtcgtcgt cgcaaacggc
     5581 cccagcagtc gtccgggcga cttttcgcgc gacgcacttg tagtggagaa cgaccgtgcg
     5641 ctcggcttct ctgcgaacgt caacaagggc atcgcggcga cgagcgccga gtacgtcgta
     5701 atcgcgaacc cggatgccgt gcccgagcga gactgcgtct ccgagctcgt tgctttcgcc
     5761 gatgcgcatc cacgctgcgg aatcgccggg ccaaagatga tcaatcccga cggcacgctc
     5821 caggcttcgc ggcgccgctt cccgacggtc ggcggcacga tcgtccgtcg cacgccgctg
     5881 cgctcgctgt tcccaccgtt gcgctggcag cggcggcact acctcctcga tgccccagag
     5941 gacgagccgc ttcaagtgga cacgatgctg ggcgcgttcc tgctcatgcg ccgatcgatg
     6001 ctcgacgaga tcggcggctg ggacgccggg tacaagctgt acgtggagga catcgacctc
     6061 agctaccgcg cgatgaaggc cgggtgggaa cgctggtggg tgccgagtgc cgtcgtccac
     6121 cacgaatacc aggccgtaat cgacaagcac ttcctcaccc gccggaacct ctggcacctg
     6181 cacgcgatgc ttcgcttcct gcgcaagtac cccgaacggc tcttcgccat tcgatgaccg
     6241 gagggaaata cgacgcgcag gcggaacgct ggtccgagac ggcgtacgca gatccggcga
     6301 cgtatctggc tcaccgcgcc cgtctcgtgt tctccgtcgg accgcggctc gagccaggcg
     6361 acacggtcct ggatctggcc tgcggcgacg gcggcctcgc ggatcacctg caggccgtcc
     6421 gctattcggg cgtggatgca agcccggaga tggtcagggc cgccacccag cgcggccgca
     6481 acgtcgtcct cggcgacctg aacgactacg agccgccgca tccggttgca gttacgacgt
     6541 gctttcgggc gatctactac gcccacgacc gtccgggctt cttccgacgc gtcgcggcgt
     6601 atacggagcg gaagttcgtc ttcgacctga atccgcgcca gtaccgggtc gaggacgtcc
     6661 gggccgactt gcgtttcgca gggttcgatc agctcgagct acgtccgttc ttctcgtcgc
     6721 agcgattcgc gctcccgcgg ccgttggccg gcgcgctcca tttcctcgaa cggagtggac
     6781 cgctggcagg gctcctgctc gcggtgcgct tcagctactt ctgcgcggct ttccgcggcg
     6841 gcaagaacac gtagcgcacc tgcgccccga ggtgacggtt gtcgctctgg tccgctgaca
     6901 gctctttcgg aacaaaggtc ggcttgaccg tcacttcaac ccggaagcgg ggcccggggg
     6961 cgtggaaggt gaggactcgt tgccccttgc tgcggaggtc gaagcgcctg acctcggtcg
     7021 gtttaccgag atgtggctgg ttgtcgtcgc cgacgacgat ggggccgatc gcgaccgtga
     7081 cgtgcccggt cgtcgcgtgt ggcccttggg gtggcccgga caacagaacg cgcatgcgtc
     7141 ccgcgcggtt gtcctcggtc gcgtagcgcg tgtaggagct gaacgagccc atccacccgt
     7201 ccgggtagac gtcggtcact gcgccgccca acctgagcgg cggctcgatc ttgacaagcc
     7261 gccacttctc gagtccacca cctgcgcggt ggagatggat cgcgaacgtc ttcccgacga
     7321 cgtcgatgcc ttgctcctcg accacgtacg gataacccca gttgccgcat cgaccgtggc
     7381 tgcacagcgc gccgtcggac gggcgaggat ccggcgtcag aaacggccct gggccctgcg
     7441 ccgttccgtc aaggctccag accgccttga tcgaccggtt ccaaaattct gtcagccact
     7501 cgccgttctg atcagccatt tgctgtccga tgtagagcgt cggtgccccg cgagttacgt
     7561 cgtcgaccca ggtgaacggt ggcctgatgt tctccccgaa gcggtccgag aagcgattcg
     7621 atgcggaggc gaacgcgaac tcacccgtga ggttccaccc gatgacggcc gccgcgaccg
     7681 caacagcgac ccaggcagca cggcgtcgta agacgcgcgg tgcgagcaac agt
//
LOCUS       DASVEU010000162        12693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_502684,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000162 DASVEU010000000
VERSION     DASVEU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502684"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="cysC"
                     /locus_tag="VF225_06460"
     CDS             complement(<1..483)
                     /gene="cysC"
                     /locus_tag="VF225_06460"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX7144935.1"
                     /translation="MTDHIYAHEHPETERRREGGFTLWFTGLSGSGKTTIAHLVGPAI
                     EERGPIVEYLDGDTVRTHLSKGLGFSKEDRDANIERIGWVAARLTRHGAAVIAAAISP
                     YEETRRRARDLVEEWGTFVEVHVATSVEECARRDVKGLYEKAFKGEIKGFTGVDDPYE
                     E"
     gene            complement(476..1186)
                     /locus_tag="VF225_06465"
     CDS             complement(476..1186)
                     /locus_tag="VF225_06465"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX7144936.1"
                     /translation="MATAPALQLPDVEAMTAEEALLWAYEQFGDRMCLTCSWQRQSSV
                     LVHMVFGELGLNIPVVELDTQLFFKETYETRDRLVERYGLTLERPEVITVARQAIQEN
                     PNLWERDPDRCCHIRKVEPLERALAPYDAWITGIRREQALTRADAERVEWSERYGVWK
                     IQPVVDWDAKRVQAYIHVNEIPYNPLHDAGYPSIGCMPCTRPVLVGEDERAGRWSGSD
                     KLECGIHGNTRLVKESND"
     gene            complement(1155..1919)
                     /locus_tag="VF225_06470"
     CDS             complement(1155..1919)
                     /locus_tag="VF225_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7144937.1"
                     /translation="MSAFELGLAPRPGGLTHVIDKGLGPRGWEDVLETSGDFIDIVKL
                     GWGTAYVTRNLRAKLDVLSGKPVVIGGTFFEVVFVQDKLEGYKRWLQGLGLTHVEISD
                     GVIDLPRERKLELIEEFARDFTVLSEVGSKDADVEFTPDEWTKWLREELDAGAWKVIT
                     EAREGGTAGIFSKSGDMRTELVGEIAEAVPLEDVVFEAPTKAAQAWFIKQLGPAVNLG
                     NIPPDEVIPLETLRRGLRGDTLKEVLLGDRAGTPAS"
     gene            complement(1906..2490)
                     /locus_tag="VF225_06475"
     CDS             complement(1906..2490)
                     /locus_tag="VF225_06475"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX7144938.1"
                     /translation="MAAVRMQFPASEEAIRELRAGDEVTVDGHIIGIRDRTQIRIFDQ
                     GIAPPMDLNGAFLLHTAPGVRKLGPGKYEKICIGTTTSARMVRFTEGLGSQYGVRAIC
                     GKGGLPDEAIEPMQRLGMVYFAIVGGAAALETTQIEEIEEVAWEELMPECLWKFRVKD
                     FGPLTVGIDSHGNSLFHDVQERAKAKLEELYERL"
     gene            complement(2558..3457)
                     /locus_tag="VF225_06480"
     CDS             complement(2558..3457)
                     /locus_tag="VF225_06480"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7144939.1"
                     /translation="MSTLTITDLEAAVEDAAAYLYVWALKDIPQDLRDALADAAKRET
                     SVPGQRVLQTILKNVNVAESEKNLVCQDTGIAVYTCRVGEHFPLHPARIYAALKTGTE
                     RATVEHPLRSNAVHTITRENTGPNTGYRLPIVHWEFIPDWDGLDVKCVPKGSGSENMS
                     FLKMCVPADGVKGVKKFVLESIVDAGGKPCPPGIVGVGIGGSADYAMYLAKEAIARPV
                     GTRNADPHVAKLEEELYELLNETGIGPMGLGGDVTVLSCHVEHADTHMTLNPVAVNYQ
                     CWAARRASAHVTANGEVEFDREF"
     gene            complement(3454..4644)
                     /locus_tag="VF225_06485"
     CDS             complement(3454..4644)
                     /locus_tag="VF225_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7144940.1"
                     /translation="MADSSFDVLVIGSGASGLAAAVSAQRAGARVALATKGALQSCNS
                     AKAQGGIQAAFGDDDSPEQHAEDVWKSSHETADRGLVEILTGEAPAAIHWLEELGVEF
                     TRENGGYRLARCGGSSRKRLLQVGDRTGHAITTALRDSAESSGIQAFPKSPLADLART
                     ENGWRARCGDHTIDATTVVLAAGGRCFRVAAERGELSTNHPGATGEVTQIAVDLGAEV
                     RDFDALQYHPNGGAWPQNMQGYSIPETTRAYGAVLLNADGEEFTDSLGPRDAVSQAIV
                     DEVEKGKGVETPDGRPAVYLDTTRISEDDANVSLPYMLRRYRAGGIDPLRERLFTYPV
                     LHYQNGGLVIDANAETTLEGLYACGEIAGGTHGRNRMMGNSLLECCVFGRRAGKAAAE
                     KAKL"
     gene            complement(4698..5660)
                     /locus_tag="VF225_06490"
     CDS             complement(4698..5660)
                     /locus_tag="VF225_06490"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7144941.1"
                     /translation="MIVRWGLAQLPTVLGELRIQEPLLVASERWAGTNLGVRTAARWT
                     EVPSDQVAAVVEHAGDGVIAVGGGSAIDLGKAISAEASVPVISVPTTYSGAEWTPFYG
                     IRDPGRRMRGGGAGAQLGGVVYEPKLTLSLPRAETVGTALNALAHSAEALYVEGHNPD
                     ADEHALAGARLIGEWLPRVVDSPHDLGARTKLLEGAMHSGMALGGSMLALGHAIAQAL
                     GGRYGLPHGAMNALALPAALRFNAEVAPEAVRRFGEALGTEDPAARVEELARLGGFER
                     LRDFGIPEEDIAEVAAATVERAGAKANPRPASAAEVEELLRSIY"
     gene            complement(5657..6553)
                     /locus_tag="VF225_06495"
     CDS             complement(5657..6553)
                     /locus_tag="VF225_06495"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX7144942.1"
                     /translation="MDTRQLAAFCAVVERKSFSQAAERLGVTQPAVSLQIRSLEQRLG
                     RQLLDRSGRRVEPTEAGRRLYASAQRLLAAEEHLLEDLDADDEGAVTGTLELGASTGP
                     GGTVVPLLLCEFQEQHPDVRVRLTVSDTQTVVDRVAERELELGIVGAGRRHRGVAFEP
                     FFRDEVVLACPSGHRFAGKTISLDDLKGEKLIVMQEGAGVRQVIEDELRKAGMRLRDL
                     DVRLELGLQESVRSAVVAGQGIAFISRLAIEADLDARRIATARVRGLDPVREIFLARA
                     SGRSETRVARAFVSFAQEHLPG"
     gene            6607..7284
                     /locus_tag="VF225_06500"
     CDS             6607..7284
                     /locus_tag="VF225_06500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7144943.1"
                     /translation="MAITPSSDSFNLRFSELTEGSETRPRDTALRAFDIALSAFFLLV
                     TLPVTLPITLVLLATSGRPLFYRGERVGRGGHVFEMLKFRTLRRNAEERLGPYLGEEL
                     VHRTGEETTAIGGWLRATQLDEIPQLWNVVRGDMSLVGPRPIRPRFFAELVEELPAYW
                     QRLVVRPGLTGFAQVRRGYETSMAEKLAHDLEWIADRSLRLYLRTLATTAWRVLRQFA
                     RGVSGRS"
     gene            complement(7285..8871)
                     /locus_tag="VF225_06505"
     CDS             complement(7285..8871)
                     /locus_tag="VF225_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX7144944.1"
                     /translation="MHFGVHEQLLLVGLLLAVAAMLVTAPLLRIPYPIFLVLGGLALG
                     FLPGIPTLQLPPDVVLIAVLPPLLYASAFFTSLRDLRANARPIGMLAIGLVIATMTVV
                     AMVAHAAIDSMSWSAAFVLGAIVSPTDPIAATAIMRRLGVPRRIVTVVEGEALVNDGT
                     ALVLYRVAITAAVSGAFSFWNASWHFVWSVVGGVGIGLVVAFLIAALRRRLDNPPVEV
                     TIALMTGYLAFIPANAANASGVLAVVTAGIYLGWRTPELTSVQTRLQGVAVFEILFFV
                     LNALLFALVGLQLSHILDVLTGISAMTLIRYGLLTTAAVVGTRLVWAPIFLYLPKLLS
                     RRPGRERGTIGHPIVVAWSGMRGAVSLAAALAIPLSTHSGAPFPERDLIIFLTFCVIL
                     GTLVVQGLTLPLVIRTFGIEADHLDEKEDAKARIKAADAALARLEELTGEEWVRDDTA
                     ERLRGLYGFRKDRFASRFDRDDDGSIEERSVNYQRLRRELLEAERAEIVDLRRTGVIS
                     GDVERRLQRDLDLEDARLDV"
     gene            8907..10790
                     /gene="asnB"
                     /locus_tag="VF225_06510"
     CDS             8907..10790
                     /gene="asnB"
                     /locus_tag="VF225_06510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7144945.1"
                     /translation="MCGICGIASTNGSAVTDRVAAMSATLVHRGPDSFGEFSDGDIAL
                     AARRLSIIDLETGDQPIANEDGTLHVVQNGEIYNYRELRRELERAGGHRFRTSGDTEV
                     LLHLYEEHGDGFAERLRGMFAIAIWDAPRRRLVLARDRFGIKPLYYRLTDSELAFASE
                     LRALPRGEIDLDALEAFLAFNSIPAPLTIFKEIRKLPPGHLLSWADGRVELKRFARPT
                     PIDELREDEEAELVEELRSRVRDSVRSHLVSDVPVGVLLSGGVDSAFLAALAAEESSE
                     ALRTFSIGFEERSFDELADARLVAERYGTQHRELVLRPDAALLLPALADAFDEPFADS
                     SALPTYLVSQLAASDVKVALSGEGGDELFGGYYTYAADLLAARVGGLARLAGPLVERL
                     PTSTSRASFDYRAKRFVRAAHLPPLERHHGWKEIFSPELRAELTGGRSSFDPVDILRA
                     RYGETEGADELARLQDVDLGIYLVDDLLVKTDRASMAHSLEARVPYLDTVVTNLALAL
                     PTRHKIRGLSKKVLLRKAAAPLLPREIVHGKKRGFSIPAAAWLRGELEPFARETLSRA
                     TLNRQGFFSPEVVTRLLDDHVAGREDRSRQLWGLLAFTLWHERHVERHPHEAARPEVL
                     VER"
     gene            10805..11869
                     /gene="wecB"
                     /locus_tag="VF225_06515"
     CDS             10805..11869
                     /gene="wecB"
                     /locus_tag="VF225_06515"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7144946.1"
                     /translation="MASRPLVVHVVGARPNYMKVAPVYAELERLGNVEQRLIHTGQHY
                     DALMKDVFFAELPLPRPHVELDAQTTEDALDDLERVFGELQPELVVVAGDVNSTLAGA
                     VAASKCGMPVCHIESGLRSGDWSMPEERNRVITDHLSTLLLTHSDDADKNLIAEGLTD
                     RISFVGNTMIDTLRANEHAALALEAWREFGLEPRGYVLVTLHRPSLVDDADLLARTIA
                     ALDDVAESLPVIFPVHPRTRRNLDGSTRVLLTEPLPYRQFLSLEAKAAAVVTDSGGIQ
                     EETTVLRIPCFTLRQNTERPITIELGTNRLLGLEPELLREVPTWLAEPKHGQIPPLWD
                     GHAGERAAEAIERLLVGVNA"
     gene            11884..>12693
                     /locus_tag="VF225_06520"
     CDS             11884..>12693
                     /locus_tag="VF225_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX7144947.1"
                     /translation="MTASAHVLVFRPLIELLRERGDEVEITARDYAQTLQLLELHGLE
                     ATVVGRHGGRSRLGKARSLASRLRALRKWARPRGFDLALAHGSHELTMTARRLGIPSA
                     TTHDYEFATLQHHLGMRAATKVVVPDSIPVERLARFGARPPKLLRYPGLKEEYYLSDF
                     EPDPAVPERLGVDRNRVLVVLRPPPDVSLYHRHSNPLFPQTLGFLGNLDTVHAVVLPR
                     TEEQREYVRELALPSVIVPDRAVDAQSLIAAADVVVSAGGTMNREAAALGVP"
BASE COUNT         2051 a         4398 c         4180 g         2064 t
ORIGIN      
        1 ctcctcgtac ggatcgtcga cgcccgtgaa gcccttgatc tcgcccttga acgccttctc
       61 gtacaggccc ttgacgtcgc ggcgggcgca ctcctcgacc gaggtcgcaa cgtgcacctc
      121 gacgaacgtg ccccactcct cgacgaggtc gcgcgccctc cggcgggtct cctcgtacgg
      181 ggagatcgca gcggcgatca cggcggcgcc gtggcgggtc agccgcgcgg cgacccagcc
      241 gatgcgctcg atgttcgcgt cacggtcctc tttcgagaag ccaaggcctt tagagagatg
      301 cgtgcggacc gtgtccccgt cgaggtactc gacgatcggc ccgcgctctt cgatcgcggg
      361 gccgacgaga tgcgcgatcg tcgtcttgcc ggaacccgac agccccgtga accagagcgt
      421 gaaaccgccc tcgcgcctgc gctccgtctc cggatgctcg tgcgcgtaga tgtgatcagt
      481 cattcgactc cttcacaagt cgcgtattgc catggatgcc gcattcgagc ttgtccgagc
      541 cggaccaacg ccccgcgcgc tcgtcctcgc cgacgaggac gggacgcgtg cagggcatgc
      601 agccgatgga cggatagccc gcatcgtgca gcgggttgta cggaatctcg ttcacgtgga
      661 tgtaggcctg gacgcgcttc gcgtcccagt ccaccacggg ctggatcttc cagacgccgt
      721 agcgctccga ccactcgacg cgctctgcat ctgcgcgggt cagcgcctgc tcgcggcgga
      781 tgccggtgat ccaggcgtcg tacggcgcga gggcacgctc gagcggttcg accttgcgga
      841 tgtgacaaca gcggtcgggg tcgcgctccc acaggttcgg gttctcctga atcgcctgcc
      901 gcgcaacggt gatcacttcg gggcgttcga gcgtgagccc gtagcgctcg acaagacggt
      961 cccgcgtctc gtaggtctcc ttgaagaaga gctgcgtgtc cagctcgacg accgggatgt
     1021 tcaggccgag ctcgccgaac accatgtgca cgagcacgga ggactgtcgc tgccacgagc
     1081 acgtgaggca catgcggtcg ccgaactgct cgtacgccca gagcagggcc tcttccgccg
     1141 tcatcgcctc gacgtcagga agctggagtg ccggcgcggt cgccaagcag cacctccttc
     1201 aacgtgtcgc cgcggaggcc gcgacggagc gtctcgagcg gaatgacctc gtccggcggg
     1261 atgttgccga ggttcacagc gggcccgagc tgcttgatga accacgcctg cgcggccttg
     1321 gtcggcgcct cgaagacgac gtcctcgagt ggcacggcct ctgcgatctc accgacgagc
     1381 tccgtgcgca tgtccccgga cttgctgaag atccctgcgg tcccgccctc gcgcgcctcg
     1441 gtgatcacct tccaggcgcc cgcgtcgagc tcctcgcgca gccacttcgt ccattcgtcc
     1501 ggcgtgaact cgacgtcggc gtccttcgac cccacctcgg agagaaccgt gaagtcgcga
     1561 gcgaactcct cgatcagctc gagcttgcgc tcgcgcggga ggtcgatcac gccgtcggag
     1621 atctcgacgt gcgtcagacc gagcccctgg agccagcgct tgtagccctc gagcttgtcc
     1681 tgtacgaaga cgacctcgaa gaacgtgccg ccgatgacga ccggctttcc gctcagcacg
     1741 tcgagcttcg cgcgcaggtt ccgggtcacg taagcggtgc cccagccgag cttcacgatg
     1801 tcgatgaagt cgccggacgt ctcgagcacg tcctcccagc cacgcgggcc gaggcccttg
     1861 tcgatcacgt gcgtgaggcc gccgggacgt ggcgccagac ccagctcaaa ggcgctcata
     1921 gagctcctcc agcttggcct ttgcgcgctc ctgcacatcg tggaagagcg agttcccgtg
     1981 cgagtcgatg cccacggtga gcgggccgaa gtctttcact cggaatttcc agaggcactc
     2041 gggcatgagc tcctcccacg cgacctcttc gatctcctcg atctgcgtcg tctcgagtgc
     2101 ggccgccccg ccgacgatcg cgaaatagac catgcccagc cgctgcatcg gctcgatcgc
     2161 ctcgtcggga agaccgcctt tcccgcagat cgcgcggact ccgtactgcg aaccgagccc
     2221 ttcggtgaag cgcaccatcc gcgcagacgt cgtcgtgccg atgcagatct tctcgtactt
     2281 cccggggccg agcttgcgca cgccgggcgc cgtgtgcagc aggaacgcgc cgttgagatc
     2341 catgggcggc gcaatgccct ggtcgaagat ccgaatctgc gtgcgatcgc ggatgccgat
     2401 gatgtgaccg tcgacggtca cctcgtcccc ggcgcgcagc tcgcgaatcg cctcctcgct
     2461 cgcgggaaac tgcatgcgaa ccgccgccat ctaactcacg tcccaaagag ccggggtcag
     2521 acccttgcag ttcgggccgt ggcttcggcg gccacgttta gaactcacgg tcgaactcaa
     2581 cttccccatt cgcagtgacg tgggcactcg cacggcgcgc ggcccaacac tggtagttga
     2641 ccgccaccgg gttcagcgtc atgtgcgtgt cggcatgctc gacatggcat gagagcaccg
     2701 tcacgtcgcc gccgaggccc atcgggccga tgcccgtctc gttgagcagc tcatagagct
     2761 cctcctcgag cttcgcgacg tgcggatcgg cgttgcgcgt gccgaccggc cgcgcgatcg
     2821 cctccttcgc gagatacatc gcgtagtcgg cgctgccacc gataccgacg ccgacgatgc
     2881 ccggcgggca gggcttgccg ccggcgtcga cgatcgactc gagcacgaac ttcttcacgc
     2941 ccttcacgcc gtcggcgggg acgcacatct tgaggaagct catgttctcg ctacccgatc
     3001 ccttcggcac gcacttgacg tccaggccgt cccagtcggg aatgaactcc cagtgcacga
     3061 tcggcagccg atagccggtg ttcggtccgg tgttctcgcg cgtgatcgtg tgcacggcgt
     3121 tcgagcgcag cggatgctcg acagtcgcgc gctccgttcc ggtcttcagc gcagcgtaaa
     3181 ttctggccgg atgcagggga aaatgctcgc cgacacggca cgtgtagacg gcgattcccg
     3241 tgtcctggca gacgagattc ttctcggatt cggcgacgtt gacgttcttg aggatcgtct
     3301 gcagcacgcg ctgccccggc accgacgtct cgcgctttgc tgcatcggca agcgcgtcgc
     3361 gcaggtcctg cgggatgtcc ttcagcgccc agacgtagag gtacgccgca gcgtcttcga
     3421 ccgcagcctc gagatcggtg atcgtgagag tgctcatagc ttcgctttct ccgcagcggc
     3481 cttgcccgcg cggcgtccga acacacaaca ttcaagaagc gaattcccca tcatgcggtt
     3541 acggccgtgc gtgccgccgg caatctcgcc gcaggcgtag agcccttcga gcgtcgtctc
     3601 ggcattcgcg tcgatcacga ggccgccgtt ctggtagtgg aggaccggat acgtgaacaa
     3661 tcgttcgcgg agcgggtcga tcccgcccgc acggtaccgg cgcagcatgt atggcagcga
     3721 cacattggcg tcgtcttcgg agatccgcgt cgtgtcgaga tacaccgccg gccgtccatc
     3781 aggcgtctcg acgcctttcc ccttctcgac ctcgtcgacg atcgcctgac tcaccgcatc
     3841 gcgaggaccg agggaatcgg tgaactcctc gccgtcggcg ttgagaagca cggcgccata
     3901 cgcgcgcgtc gtctcgggga tggagtagcc ctgcatgttc tgcggccagg cgccgccgtt
     3961 cgggtggtac tgcagcgcgt cgaaatcgcg cacctcggcg ccgaggtcga cggcgatctg
     4021 cgtcacctca ccggtcgcgc cgggatggtt cgtcgaaagc tcgccccgtt cggcggcgac
     4081 gcggaagcac cgcccgcccg ctgcgaggac caccgtcgtg gcgtcgatcg tgtgatcccc
     4141 gcagcgtgcg cgccagccgt tctcggtccg ggcgaggtcg gcgagcggtg acttgggaaa
     4201 ggcctggatg ccggagctct cggcggagtc gcgcagcgcg gtcgtgatcg catgtcccgt
     4261 gcggtcgccg acctgcagga gccgcttgcg cgacgagccg ccgcagcggg cgaggcggta
     4321 accgccgttc tcgcgggtga actcgacacc gagctcctcg agccagtgga tcgcggccgg
     4381 cgcttcgccc gtcaggatct cgaccagacc ccgatcggcc gtctcgtgcg aggacttcca
     4441 gacgtcctca gcgtgctgct cgggcgagtc gtcgtccccg aaggccgcct ggatgccgcc
     4501 ctgtgccttg gcggagttgc aggactggag cgcgcccttg gtcgccagcg cgactcgcgc
     4561 gcccgcccgc tgggcggaga cggctgctgc gaggcctgaa gccccgctgc cgatcacgag
     4621 cacgtcaaag gaggagtccg ccacgacacg cagaccatat cacgatccga ctaatccgac
     4681 gtattagtcg ttcttattca gtagatcgag cgcaagagct cctcgacctc ggccgcgctc
     4741 gccgggcgtg ggttcgcctt ggcaccggcg cgctcgaccg tcgcggctgc aacctcggca
     4801 atgtcttcct ccggaattcc gaagtcgcgc agccgctcga agccgccgag acgggcgagt
     4861 tcctccactc gggcagccgg atcttccgtc ccgagcgcct ccccgaatcg gcgcacggct
     4921 tcgggcgcga cctcggcgtt gaagcgcaga gcggcgggca aagccagcgc gttcattgcg
     4981 ccgtgtggca agccgtagcg cccgccgagc gcctgcgcga tcgcgtgacc gagcgcgagc
     5041 atggagccac cgagcgccat ccccgagtgc atcgctccct ccagcagctt cgtccgcgca
     5101 ccaaggtcgt gcggcgagtc gacgacgcgc gggagccatt cgccgatcag ccgggctccg
     5161 gcaagcgcgt gctcgtccgc gtccggattg tggccctcga cgtacaacgc ttcggcgctg
     5221 tgagcgagcg cgttgagcgc agtgccgacg gtctcggcgc gcggaagtga caacgtcagc
     5281 ttcggctcgt agaccacgcc gccgagttgc gcgccggcgc cgccgccacg catcctccgc
     5341 ccgggatcgc ggatcccata gaagggggtc cattctgccc cgctgtaagt ggttggcacc
     5401 gagatgacag gcacactcgc ctcggccgag atcgccttgc cgaggtcaat ggcgctgcca
     5461 cctcccacgg cgatcacacc gtcgccagcg tgctccacca cggcggcgac ctgatcggat
     5521 ggaacctcgg tccagcgcgc tgcggtgcgc acgccgaggt tcgtgcctgc ccagcgctcg
     5581 ctcgccacga gcagcggctc ctggatgcgg agctcgccga gcacggtggg cagctgggca
     5641 aggccccagc ggacgatcac cccggcaggt gctcctgcgc gaaagagacg aatgcgcggg
     5701 cgacgcgcgt ctcgctccgc ccgctggccc gcgcgaggaa gatctcgcgc accgggtcga
     5761 gcccgcggac gcgggcggtc gcgatgcggc gcgcgtcgag gtcggcttcg attgcgaggc
     5821 gggagatgaa ggcgatgcct tgtccggcaa caacggcgct acgcacggat tcctgcaggc
     5881 cgagctcgag gcggacgtcg aggtcgcgca gccgcatgcc cgccttgcgc aactcgtcct
     5941 cgatgacctg acgaacgcct gccccctcct gcatcacgat cagcttctcg cccttcagat
     6001 cgtcgagcga gatcgtcttc ccggcgaagc gatgacccga cgggcaagcg agcacgacct
     6061 cgtcgcggaa gaacggctcg aaggcgacac cgcggtggcg gcgtcccgcg ccgacgatcc
     6121 caagctcgag ctctcgttcg gcgacgcggt cgacgacggt ctgcgtgtcc gagacggtca
     6181 gccgcacgcg cacgtcggga tgctgctcct ggaactcgca gagcagtaag ggaacgaccg
     6241 tgccgccggg ccccgtcgat gcgccgagct caagcgtgcc cgtcactgcg ccctcgtcgt
     6301 ccgcgtcgag gtcttcgagc agatgctcct cggcggcgag caggcgctgc gccgaggcgt
     6361 agagccggcg gccggcctcg gtcggctcga cccggcgtcc cgagcggtcg agcaactggc
     6421 gcccgaggcg ctgctcgagc gaccggatct gcaggctgac ggccggttga gtcaccccga
     6481 gccgctccgc cgcctgggag aagctcttcc gctcgaccac ggcgcagaat gcggctagct
     6541 ggcgcgtgtc cataagcatt cattatgcaa cccggcgctg ggtggagcag gctctacgat
     6601 caagaggtgg cgatcacgcc gtcgagcgac tccttcaacc tgcggttctc ggagctcacc
     6661 gaaggaagtg agactcggcc tcgtgacacg gcgctgcgcg cgttcgacat cgcactctcc
     6721 gctttcttcc tcctcgtgac gctgcccgtc acgctgccga tcaccctcgt tctgctcgca
     6781 accagcggcc ggccgctctt ctaccgtggc gagcgcgtcg ggcgtggcgg gcacgtgttc
     6841 gagatgctca agttcaggac gctgcggcga aacgccgagg aacggctcgg cccctacctc
     6901 ggcgaggagc tcgtacaccg gacgggcgag gaaacgaccg cgatcggcgg ctggctgcgc
     6961 gcgacgcagc tggacgagat tccgcagctc tggaacgtcg tgcgcggcga catgagcctc
     7021 gtcgggccta gaccgatccg gcctcgcttc ttcgcggagc tcgtcgagga gctgcctgcg
     7081 tactggcaac ggctcgtcgt ccgtccaggg ctcaccgggt tcgcgcaggt gcgtcgcggc
     7141 tacgagacct cgatggcgga gaagctcgcg cacgacctcg agtggatcgc cgatcggtcg
     7201 ctgcgcttgt atctgcgcac actcgccacc acggcgtggc gcgtgctgcg ccagttcgcg
     7261 cgcggtgtgt caggacggag ctaactagac gtcgagccgg gcgtcctcga ggtcgaggtc
     7321 gcgctggagg cgtcgctcga catccccgct gatcacaccc gtgcggcgga gatccacgat
     7381 ctcggcgcgc tcggcctcga gcaattcccg gcggagacgc tggtagttga ccgaccgctc
     7441 ctcgatgctg ccatcgtcgt cgcgatcgaa acgcgaggcg aacctgtcct tgcgaaagcc
     7501 gtacaacccg cgcaaacgtt cggccgtgtc gtcgcgaacc cactcttctc cggtcaactc
     7561 ctcgagtcgc gcgagtgcag cgtcggccgc cttgatccgc gccttcgcat cctccttctc
     7621 gtcgaggtgg tcggcctcga tcccgaacgt cctgatgacg agggggagtg tcaatccctg
     7681 gaccacgagc gtcccgagga tcacgcagaa cgtgaggaag atgatcagat cccgctcggg
     7741 aaacggagcg cccgagtggg tggacagagg gatcgccagc gccgcggcga gcgacaccgc
     7801 accgcgcatg ccggaccagg ccacgacgat cgggtggccg atcgtgccgc gctcgcgccc
     7861 gggccgcctg gaaagcagct tcggcaagta caagaagatc ggcgcccaca cgagtctcgt
     7921 gccgaccaca gcggcggtcg tcagcaaccc gtaccggatc aatgtcatgg cggaaattcc
     7981 tgtcagcaca tcgaggatgt gggacagctg gaggccgacg agggcaaaca gcagagcgtt
     8041 gaggacgaag aagaggatct cgaacacggc aactccttgc agccgggttt gcaccgatgt
     8101 gagctcgggc gttcgccagc cgagatagat gccggccgtg accaccgcga gcacgcccga
     8161 cgcgttcgcc gcattcgccg ggatgaaggc gaggtacccg gtcatgaggg cgatcgtcac
     8221 ctcgacgggg ggattgtcga gccgccgccg cagtgcagcg atgaggaagg cgacaacaag
     8281 accgatcccg acgccgccca cgacgctcca gacgaagtgc caggaggcgt tccagaacga
     8341 gaaggcgccc gacactgctg cagttatcgc aacgcggtaa agcaccagcg ccgtgccgtc
     8401 gttcaccagc gcctcaccct ccaccacggt gacgattcgc cgcggcacgc ccagccgccg
     8461 catgatcgcc gtcgcggcga tcggatcggt gggcgagacg atcgctccga gcacgaatgc
     8521 agcactccag gacatggaat cgatcgcagc gtgggcgacc atggcgacga cggtcatcgt
     8581 cgcgatgacg agcccgatcg ccagcattcc gatcggacgg gcgttcgcac ggaggtctct
     8641 cagagaggtg aagaacgcgg aggcgtagag caggggcggc aagacggcga tgaggacgac
     8701 gtcaggcgga agctggagcg tcgggatccc gggcaggaag ccgagcgcga gtccgccgag
     8761 caccaggaag atcgggtaag ggatgcgcag cagcggtgcc gtcacgagca tcgccgcgac
     8821 cgccagcagc aagcccacca ggagaagctg ctcgtgcacc ccgaagtgca tcccgggaag
     8881 cctacgccgc tcctacactg ctccgcatgt gcgggatctg cggaatcgcc tcgacgaatg
     8941 gctcggccgt caccgaccgt gtcgcggcga tgagcgcgac gctcgtccac cgcggtccgg
     9001 acagcttcgg cgagttcagc gacggggaca tcgcgctcgc cgcccggcgg ctctcgatca
     9061 tcgatctcga gacgggtgac cagccgatcg cgaacgagga cgggacgctg cacgtcgtcc
     9121 agaacggcga gatctacaac taccgcgagc tgcgccgtga gctcgagcgg gcagggggcc
     9181 atcgcttccg tacaagcggc gacaccgagg tgctgctgca tctctacgag gagcacggcg
     9241 acgggttcgc cgagcggctg cggggcatgt tcgcgatcgc gatctgggac gcaccgcgcc
     9301 gccggctcgt gctcgcgcgt gaccgcttcg ggatcaagcc gctctactac cgcctgaccg
     9361 acagcgagct tgccttcgcg tccgagctac gcgcgctgcc ccgcggcgag atcgacctcg
     9421 acgccctgga ggctttcctc gcgttcaact cgatccccgc acctctgacg atcttcaagg
     9481 agatccgcaa gctgccgccc ggccacctgc tgagctgggc agacggccgc gtcgagctga
     9541 agcgtttcgc ccgcccgacg ccgatcgatg agcttcgcga agacgaggag gcggagctcg
     9601 tcgaggagct ccggtcgcgg gtccgggatt ccgtgcgctc gcacctcgtc agcgatgttc
     9661 ccgtcggagt tctgctctcg ggcggcgtcg actccgcgtt tctcgcagcg ctagccgccg
     9721 aggagagctc cgaggccttg cgcacgttct cgatcggttt cgaggaacgc tcgttcgacg
     9781 agcttgccga tgcgcgactc gttgcggagc gttacgggac gcagcaccgc gagctggtgc
     9841 tccgcccgga tgccgcgttg ctcttgccgg ccctggccga cgccttcgac gagccgttcg
     9901 cagattcgtc cgcgctgccg acgtacctcg tgtcgcagct cgcggcgagc gacgtgaagg
     9961 tcgcgctctc cggcgagggc ggcgacgagc tcttcggcgg ctactacacg tacgcggccg
    10021 acctcctcgc ggcccgtgtc ggcgggcttg cgcgcctggc gggcccgctc gtcgagcggt
    10081 tgcccacatc gacttccagg gcgagcttcg actaccgggc gaagcgcttc gtccgtgccg
    10141 cgcaccttcc tccgctcgag cgccatcacg gttggaagga gatcttctcg cccgagcttc
    10201 gtgctgagct caccggcggg cgctcgtcgt tcgatccggt cgacatcctg cgcgctcggt
    10261 acggcgagac cgagggcgcc gatgagctcg cacgcctgca ggacgtcgac ctcggcatct
    10321 acctcgtcga cgacctgctc gtgaagaccg accgcgcgtc gatggcgcac tcgctcgagg
    10381 cccgcgtgcc gtatctcgac acggtcgtga cgaacctcgc gctcgcgctg cccacgcggc
    10441 acaagatccg cggcctctcg aaaaaggtgc tcctgcgcaa agcggctgca ccgctcctac
    10501 cgagggagat cgtccacggc aagaagcgtg gcttctcgat tccggcggcc gcctggctca
    10561 ggggcgagct cgagcccttc gcaagggaga cgctttcgcg cgcgacactg aaccgccagg
    10621 gcttcttcag tcccgaggtg gtcacgcgcc tgctggacga ccacgtcgcc ggacgcgagg
    10681 atcgcagccg ccagctctgg gggttgctcg cgttcacgct ctggcacgag cggcatgttg
    10741 agcggcaccc gcacgaggcg gcacggccgg aagtgctcgt cgagcgctga cctagactcc
    10801 tcgaatggcc agccgacctc tcgtcgtcca tgtcgtcggc gcgcgcccga actacatgaa
    10861 ggtcgcgccg gtgtacgcag agctggaacg gcttggcaac gtcgagcagc ggctgatcca
    10921 cacgggacag cactacgacg cgctcatgaa ggacgtcttc ttcgcggagc ttcccctccc
    10981 gaggccgcac gtggagctgg atgcacaaac aaccgaggac gcgttggacg atctggaacg
    11041 cgtcttcggt gagctacagc cggagctcgt ggttgtggcg ggagacgtca actcgacgct
    11101 ggccggcgca gtcgccgcgt ccaagtgcgg aatgcctgtt tgccacatcg agtccggctt
    11161 gcgcagcggg gactggtcaa tgccggagga gcgcaaccgc gtcatcaccg accacctttc
    11221 gaccttgctc ctgacccaca gcgacgacgc tgacaagaac ctgatcgctg aaggcctcac
    11281 cgacaggatc tcattcgtcg ggaacacgat gatcgacacg ctccgagcga acgagcacgc
    11341 agcgctcgca ctcgaggctt ggcgcgagtt cgggctcgag ccccgaggct acgtcctcgt
    11401 caccctccat cgcccgagcc tcgtcgacga tgccgacctg ctcgcgagga caatcgccgc
    11461 gctcgacgac gtcgccgagt ccttgccggt gatctttccg gttcatccgc gaactcgacg
    11521 aaatctcgac ggctcgacac gcgtcctcct gacggaaccg ctgccgtatc ggcagtttct
    11581 ctcgctcgaa gcgaaagccg ccgcggtcgt gacggactcg gggggaatcc aggaggagac
    11641 gacggtgctc cgaattccct gcttcacgct acgccagaac accgaacgcc cgatcacgat
    11701 cgagctcggc acgaataggc tgctgggcct cgagccggaa ctgctgcgcg aggtgccgac
    11761 gtggctggcg gagccgaagc acgggcagat tccgccactt tgggatgggc atgcaggaga
    11821 gcgggctgct gaagcaatcg agcgcttgct ggttggagtt aacgcctgaa ggtctgggtg
    11881 gacatgaccg cgtcggcgca cgtactcgtg ttccggccac tgatcgagct gttgcgcgag
    11941 cgtggcgacg aggtcgagat caccgcgcgc gactacgcgc agacgttgca gctgctcgag
    12001 ctgcacgggc tcgaagcgac ggtcgtcggc cgccacggcg ggcgctcgcg gctcggcaag
    12061 gcgcgttcgc tcgcgtcacg cctgcgcgcg ttgcgcaagt gggcgcgccc tcgtggcttc
    12121 gacctcgcgc tggcccacgg ttcgcacgag ctcaccatga ctgcgcggcg gctcggcatc
    12181 ccgagtgcga cgacacacga ctacgagttc gccaccctgc agcaccacct cggcatgcgc
    12241 gccgcgacga aggtcgtagt gcccgactcg attccagtcg agcggcttgc acgcttcggc
    12301 gcccgaccgc cgaagctcct gcgctatcca ggcctgaagg aggagtacta cctctcggac
    12361 ttcgagcccg accctgccgt gccggagcgg ctcggagtcg accgcaatcg cgtgctcgtg
    12421 gtgctgcggc cgccgcccga cgtctcgctc taccaccgcc actccaaccc gctgttcccg
    12481 cagacgctcg gctttctcgg caacctcgac acggtgcacg cagtcgtcct gccgcgaacc
    12541 gaggagcagc gcgaatacgt ccgcgagctc gcgctgccgt ccgtgatcgt gccggatcgt
    12601 gctgtcgacg cacagagcct gatcgctgcg gccgatgtcg tcgtgtctgc cggcggaacg
    12661 atgaatcgcg aagccgcagc gctcggagtg ccc
//
LOCUS       DASVEU010000163         7387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_698011,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000163 DASVEU010000000
VERSION     DASVEU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698011"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            808..1149
                     /locus_tag="VF225_06525"
     CDS             808..1149
                     /locus_tag="VF225_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144948.1"
                     /translation="MDVLDEAEERIHDSDVNRPTVVLAFDDKETKRRREPEHGTPHGD
                     VDLTDSAAAPAYPRIVLHDRGRPDRVPAAIPSSYAFQSLIRRDALHDAHVTPRRPTSA
                     LAEHCTLLACR"
     gene            complement(1199..1774)
                     /locus_tag="VF225_06530"
     CDS             complement(1199..1774)
                     /locus_tag="VF225_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEX7144949.1"
                     /translation="MIVPVVEGQSEERAIGALMRRVLYELGVFDLSVSRPLRVKRQRV
                     VREGELERTIIQAERSRAGAWSVFIVLDADEACPADLGLILLQRARAQTNLAVRVVLP
                     KVEIEAWILAGVDSLRGVCGIRGDAEPPPYPETIRDAKGALTRLMEDRRGYVATDDLP
                     ALVEQLDLDLTQERSPSFAKFRRDLTSLAHA"
     gene            complement(1783..2538)
                     /locus_tag="VF225_06535"
     CDS             complement(1783..2538)
                     /locus_tag="VF225_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7144950.1"
                     /translation="MFAASATPEFRPVYDFLTGMRFYSIVPSELRKLQSPDAGEILRA
                     DGGNAAAVLKRIRVQNPDRYRRINSLLATAVSGIQSADHKSVGQWETVEFRQDVGQKH
                     PWTFDALNVSDGTLRLLGLLVAVYQPGRATLLGIEEPEATVHPAVAEQILEVLVDAAR
                     FRQVVVTTHSPDLLDFRDLRDDQLRVVRNPQNTTVIADLSDTSRRAIREHLFTTGELM
                     RAGELEGDADSALALANQQRLFGPVVSPRPRAA"
     gene            complement(3241..3885)
                     /locus_tag="VF225_06540"
     CDS             complement(3241..3885)
                     /locus_tag="VF225_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144951.1"
                     /translation="MTEDPVVEFWYELDDHFLFNVGDHPEILDAYGVIQMPDGITNLR
                     VEHRANGTYPAGFLADVTPMRAALELLSTEQLKIFDLHLSGDPQTTQRAFEEFGQGVH
                     YDARRDPGTRIHMMDASGPTNPAVGYHRWHAIIRAQIALGIDADRWTEIDRCVGLGWA
                     MQSEAQPVQSEHANPGLPAARIDELRERWLKRTSKQIDDAFDSVPYPSDAVVPA"
     gene            complement(3895..4998)
                     /locus_tag="VF225_06545"
     CDS             complement(3895..4998)
                     /locus_tag="VF225_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7144952.1"
                     /translation="MLRLRQDARAPGERDQLVFEFKDPLLELVRLLREATEVEHALLV
                     QYLYAGFSVKPQYQGIVGSAFPVTAHNLLGVAVQEMQHLDDVNQMLLAVGASPNLIRQ
                     DFPYQPTIYPFAFNLEPMSRESLAKYVWTEAPANDPFLEQLQPVLGELRPNHLGSLYA
                     TIISETEKLIADPPSPDIDLTPWPAKLKFIKGEGEVAHFVFFKELFLGTHAGFGGLAN
                     VWDLAKDDPSYPALDLQVNPTPFVTLPNTITDETWAAIAWLSDLHYWITLVLLDIGYR
                     YGSATAMALAKSHMTGPLLMLGRHLATLGCGTPFDPLSVGYNLGHDREATKRLLGHLL
                     AEAQAETEALRSDLPAAFPFAQTPFTISKLSQL"
     gene            complement(5127..6641)
                     /gene="gcvPB"
                     /locus_tag="VF225_06550"
     CDS             complement(5127..6641)
                     /gene="gcvPB"
                     /locus_tag="VF225_06550"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7144953.1"
                     /translation="MSAISVGGATPQQREHATTIFEKGAPGRRAFSPPPLDVPEVDGL
                     IPAHLRRAAPPRLPEASEPEIVRHYVNLSKRNFDLDSGFYPLGSCTMKHNPRLHERVA
                     ALPGHSRLHPLQDPRRAQGALELMFELQQALGEIAGLPHVSLQPSAGSHGELAGVLLT
                     RAYHADRGEDRPKVLTPDSAHGTNPATVTMAGYEVVKVALDADGGVDVDDLRAKADEH
                     TACLMLTNPSTLGIFDPNIERIAEIVHDVGATLYYDGANLNAIMGYSRPGDMGFDIVH
                     FNLHKTFTQPHGGGGPGAGPIAVSERIEPFLPRPQVVRRPPANGGPPTFDLDDDRPKS
                     IGKLRGFQGNYGVFVRTYAYIRSLGAEGLKEISEVAVLNANYVLARLRGEVGEHLPLA
                     FGERCMHEFVLSGAPLKKQLGIRTLDLAKRLLDHGMHPPTVYFPLVVDEALLIEPTET
                     ETRETLDAFVDAIVAIVREAAEDPEVARNAPYTTPVRRLDEAGAARRPRVRQAL"
     gene            complement(6638..>7387)
                     /locus_tag="VF225_06555"
     CDS             complement(6638..>7387)
                     /locus_tag="VF225_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144954.1"
                     /translation="LGTVEEVQTLAAAAHEHGALVVVVADPLTLGVLEAPGNQGADVC
                     VGEGQSLGNRLDYGGPSFGFFAATEKHLRSMPGRIAGETTDVDGRRGFVLTLQTREQH
                     IRRERATSNICTSQALNALAGVVYLSWLGRRGIVELGELMLQRTHYARETLSALDGVR
                     KLHDQPVVREFALRLDVDGLESVSSVVERCAAAGVNAGYALGRSYEEYPDGLLVAITE
                     QRSRADIDRFAEVLGNAVAAERAPQAAGVAL"
BASE COUNT         1186 a         2418 c         2553 g         1230 t
ORIGIN      
        1 cggggaatcg tgcttctcgg atgtgatgcg agagaaaccc gttcgtcctg gcggctcgtg
       61 cttgtcgcgc acgcgaccgc tcgtggaggg ccttcggccg gagcgttccg ttcgtcggtc
      121 gctgtcgcga cagccacgac aaagaggagc agcggggctg gccagctcct ccgagagacc
      181 ctagacccgg tgctgcatcg tcggccgagg ctccgctcac ggacgcctgc ggccgctccc
      241 tcgcgcctcc ttgcgcgcgg cgtcagtccg tgtcagcgca cggccagccg tccggcagct
      301 taggtttgag cgtactggga tgacacccag atgctggcga gctgcgcgac ttgtcgatca
      361 agctccgccc ggtcaggagc gagcacgccg aatggcgtat cggtcgcgcc gacgtagacg
      421 gggatccagc gatgcgccgg cgtgccgcca gcgaacgaga cggcgcttcc tgtgcttcgc
      481 gcttggatgc cggcgtctcg aaacgctcgg aatgcgggct cggagaaggc atcggggtca
      541 gtcggatctg cgagacgata agcctcgaca gggaactcat cggcgaggtt cggctcagct
      601 cgcacgcggt atccggcctc tatcaggacg tcccgtgccg gccgcacctg cggagtggcg
      661 acactgagcc ggagcgagcg cgttcccgga ggcatccatc gatcgagctc agaacgacca
      721 atgacgcgca cacgctgccg cttctcctcc gcccgcgcct gcgcggttgg agtgattctg
      781 ttcgccgtga cgagaagacc aagacgcgtg gacgtcctcg acgaggccga agaacgcatc
      841 cacgactccg acgtcaatcg gccgacggtg gttcttgcat tcgacgacaa agagacgaag
      901 aggcgtcgcg agcccgaaca cggaaccccg catggcgatg tcgatctgac ggattcggcc
      961 gctgcgcccg cgtatccgcg catcgtgctc cacgaccgcg gccgacccga ccgtgtgccg
     1021 gccgcaatac cgagttcgta cgctttccag tcgctcatcc gccgcgacgc gctacacgac
     1081 gcccacgtaa cgccccgccg tccgacttcg gctttggccg agcactgcac tctgctggct
     1141 tgccggtagt gttggaactc gcgcgaaagg ggagcggcgc tctgtgatcc atgtgggtct
     1201 aggcatgagc gaggcttgtc aggtcgcgcc ggaactttgc gaaggacggc gaacgttctt
     1261 gcgtcaggtc tagatcgagc tgctcgacca gtgctgggag gtcgtctgtc gccacgtagc
     1321 cgcgccgatc ctccatgagg cgagtgagcg ctcccttggc atcgcggatg gtttcgggat
     1381 agggcggtgg ctctgcgtcg ccacggattc cgcaaacccc tctcaacgaa tccacgcccg
     1441 ccaaaatcca agcttcgatc tcgaccttgg gaagcacaac gcgtacagcg aggttggtct
     1501 gcgcgcgggc gcgctgcaag aggatcaagc ctaggtctgc cgggcaagcc tcatcggcat
     1561 cgagcacgat gaacacggac cacgctccag cgcgggaccg ctcggcttgg atgatggttc
     1621 tttccagttc gccctcgcga acgactcgct gacgctttac gcgcaacgga cgcgagaccg
     1681 ataagtcgaa cacgccaagc tcgtaaagga cacgccgcat cagcgcaccg atcgcgcgtt
     1741 cctccgactg accttcgacg accgggacga tcaccgaagt ggttacgccg cacgtggtcg
     1801 cggcgacacg acgggaccga agagtctctg ttggttggct agcgccagcg cgctatccgc
     1861 gtcgccctcc aactctccgg cccgcatgag ttcacccgtt gtgaaaagat gttctcgaat
     1921 ggcccgacga cttgtgtcgg aaaggtcggc gatgacggtg gtgttctgcg gatttctgac
     1981 aactcgcagc tggtcgtctc gcaggtctcg gaagtcaagg agatcgggac tatgagtcgt
     2041 tacaacgacc tgcctgaatc gggcggcgtc gaccagtacc tcaaggattt gctcggcaac
     2101 cgcaggatgc actgttgcct ctggctcctc gatcccaagg agcgtggcgc gccctggctg
     2161 atagacggcg acgagcaggc cgagcagccg cagggtgccg tcggagacgt tcagcgcgtc
     2221 gaaagtccac ggatgcttct gtcctacgtc ttggcggaac tcgactgtct cccactgtcc
     2281 gacggacttg tgatcggcgg actgaatgcc gctcaccgcc gtagccaaga gggaattgat
     2341 gcgccgatac cgatccgggt tctggaccct aatgcgcttc agcacggccg cagcgttccc
     2401 tccgtcggca cggaggattt cgccggcgtc cggcgattgg agttttctca gctcgctggg
     2461 aacgatcgaa tagaaccgca taccggtcag aaagtcgtag accggcctga actcgggtgt
     2521 cgctgaggcc gcgaacaacg cgagacggtc gggggcgacg cttggtctga tgccttcgat
     2581 cgggatcttg aactgacccc gctcgacctc gaaggaatgg gcttggccca tgaactcttc
     2641 aacgtggcag cgctcgcgag cgaccgagaa ctgctcgccc ttctcagctg cgatgcggcc
     2701 accgtgttcg caccgaggtc agcgacaact cggattccga tgtgcgttgg atggccgcca
     2761 gaacggcgcc gcacggaggc gagacccccc ccggcccttg aaggcgagct cgacggagtc
     2821 ggtaagagag tcgctgacga aagctaaggc gtcgaggaag ttgctctttc cggcgccgtt
     2881 agcccctacg aggatcgtca ggccgcccat agccaccgtg gcctggccga tgctcttgta
     2941 gttgcggatg tggacgtcgc gaactcgtcc cacgccttcg acgccgttgg acgagtcggc
     3001 ttggacctgg gcatcgactg aagacacggc ccgaagtatc gccgacgagc ggtcgtgcaa
     3061 cagcgccggc gctcggggct tgagcagatc gaggccgatg ggcgaagtac ccgcgcttct
     3121 cgtttcgcgt gattgcggcg gtcgcaccag agcgcccctc atcgcgcaaa ggcggcgcgc
     3181 cctcgcacta gctgcgccac gttttcccag cgatcgctgg gtagctgaac gtcgggaccg
     3241 ctatgccggc acaaccgcgt cgctcgggta cgggacgctg tcgaaggcat catcgatctg
     3301 ctttgaggtg cgcttgagcc agcgctcgcg cagctcgtcg atccgcgcgg ccggcagccc
     3361 cggattggcg tgttcgctct gtacgggctg cgcctcggac tgcatcgccc agccgaggcc
     3421 gacacagcgg tcgatctcgg tccagcgatc ggcgtcgatg ccgagcgcga tctgggcgcg
     3481 gatgatcgcg tgccaacggt gatagccgac cgccgggttc gtcgggcccg acgcgtccat
     3541 catgtgaatc ctcgtacccg ggtcgcggcg tgcgtcgtag tggacaccct ggccgaactc
     3601 ctcgaaggca cgctgcgtcg tctgcgggtc accactgagg tggaggtcga agatcttcag
     3661 ctgctcggtc gagagcagct cgagcgcggc gcgcatgggc gtcacgtcgg cgaggaatcc
     3721 ggccgggtag gtcccgttcg ctcgatgctc gacacggagg ttcgtgatgc cgtccggcat
     3781 ctggatcacg ccgtaggcgt cgaggatctc cgggtggtcg ccgacgttga agagaaagtg
     3841 atcgtcgagc tcgtaccaga actcgaccac cggatcctcg gtcatcaatt cacctcacag
     3901 ctgtgacagt ttggagatcg tgaagggcgt ctgggcgaag ggaaaggcag ccggcaggtc
     3961 gctgcgtagc gcctcggtct cggcttgcgc ctcggcgagc aagtggccga gcaggcgctt
     4021 ggtcgcctcg cggtcgtggc cgaggttgta gccgacgctg agcggatcga acggcgtacc
     4081 gcatccgagc gtcgcgaggt ggcggccgag catgagcaat ggcccagtca tgtggctctt
     4141 cgcgagcgcc atggcggttg ccgagccgta gcggtacccg atgtccagca gcaccagcgt
     4201 aatccagtag tgcaggtcgc tcagccacgc gatagccgcc cacgtttcgt cggtgatcgt
     4261 gtttggcagc gtgacgaacg gcgtcgggtt gacctgcagg tcgagtgccg ggtagctcgg
     4321 gtcatccttc gcaaggtccc acacgttcgc caagcccccg aagcccgcgt gcgtgccgag
     4381 gaacagctcc ttgaagaaca cgaaatgggc aacctcgccc tcgcccttga tgaacttgag
     4441 cttcgccggc cagggcgtca ggtcgatgtc cggcgacggc ggatcggcta tcagcttctc
     4501 cgtctcggag atgatcgtgg cgtacaggct gcccaggtga ttcgggcgca gctcgccgag
     4561 caccggctgc agctgctcga ggaacggatc attcgccggc gcctcggtcc acacgtactt
     4621 cgcgaggctc tcacgcgaca tcggctcgag gttaaatgcg aagggataga tcgttggctg
     4681 gtagggaaaa tcctggcgga tgagattcgg cgaggcgccg accgcgagga gcatctggtt
     4741 gacgtcatcg agatgctgca tctcctgcac ggctacgcca aggaggttgt gcgctgtcac
     4801 cggaaacgcg gagccaacga taccctggta ctggggtttc accgagaatc cggcgtagag
     4861 gtactggacg aggagcgcgt gctcgacctc ggttgcctcg cgcagcaggc gcacgagctc
     4921 cagcagcggg tccttgaact cgaacacgag ctggtcgcgc tcacctggcg cacgcgcgtc
     4981 ctgccggagc cgcagcatca ccctcctcgt cgtcgctacc gtggctgcga ttctaccgcc
     5041 cgggacggcg ggacagcccc acgatatggc cggcaccgcg cacccgcacc cccggtgacg
     5101 cgcggctcgc gacgaccgcc gcgcggctac agcgcctggc ggacccgcgg gcgccgcgcg
     5161 gcgccggcct cgtcgaggcg ccggacgggc gttgtgtagg gggcgttgcg ggcgacctcc
     5221 gggtcctcgg ccgcctccct gacgatggcg acgatcgcgt cgacgaacgc gtcgagcgtc
     5281 tcgcgcgtct cggtctcggt cggctcgatc agcagcgcct cgtcgacgac gagcgggaag
     5341 tacaccgtcg gcgggtgcat gccgtggtcg agcaggcgct tggcgagatc cagcgtgcgg
     5401 atgccgagct gcttcttcag cggcgcgccg gacagcacga actcgtgcat gcagcgctcg
     5461 ccgaaggcca gcggcagatg ctcgccgacc tcgccgcgca accgtgcgag gacgtagttc
     5521 gcgttgagca ccgccacctc gctgatctcc ttcaggccct cggcgccgag cgaccggatg
     5581 taggcgtagg tccgcacgaa gacgccgtag ttgccctgga acccgcggag cttgccgatc
     5641 gacttcgggc ggtcgtcgtc gagatcgaac gtcggagggc caccgttcgc agggggtcgg
     5701 cggaccacct gcgggcgcgg gaggaagggc tcgatacgct cgctgacggc gatcgggccg
     5761 gcccccgggc cgccaccgcc gtgtggctgg gtgaacgtct tgtgcaggtt gaagtgcacg
     5821 atgtcgaagc ccatgtcgcc aggccgcgag tagcccatga tcgcgttgag gttcgcgccg
     5881 tcgtagtaga gcgtcgcgcc gacatcgtgg acgatctcgg cgatccgctc gatgttcggg
     5941 tcgaagatcc ccagcgtgct cgggttcgtg agcatcaggc aggcggtgtg ctcgtcggcc
     6001 ttcgcgcgca ggtcgtcgac gtcgactccg ccgtcggcgt cgagcgccac cttgacgacc
     6061 tcgtagccgg ccatcgtcac cgtggcgggg ttcgtgccgt gggcgctgtc gggcgtcagc
     6121 accttcggtc ggtcctcgcc gcggtcggcg tggtaggcgc gcgtcagcag cacgcccgcc
     6181 agctcgccgt gcgagcctgc cgagggctgc agcgagacgt ggggcaggcc ggcgatctcg
     6241 cccagcgcct gctgcagctc gaacatgagc tcgagcgcgc cctgcgcgcg acgcgggtcc
     6301 tgcagcgggt gcaggcggga atggcccggc agcgccgcga cgcgctcgtg caggcgcggg
     6361 ttgtgcttca tcgtgcacga gccgagcggg tagaagcccg agtcgagatc gaagttgcgc
     6421 ttggagaggt tgacgtagtg acgcacgatc tccggctcgg aggcctcggg cagcctcggc
     6481 ggcgcggcgc ggcgcaggtg cgccgggatc aggccgtcga cctcgggcac gtcgagcggc
     6541 ggcggcgaga aggcgcggcg cccgggcgcg cccttctcga agatcgtggt cgcgtgctcg
     6601 cgctgctgcg gcgtggcgcc gccgacggag atggcgctca tagggccacc ccggcagcct
     6661 gcggcgcccg ctcggccgcg acggcgttgc cgagcacctc ggcgaatcgg tcgatgtccg
     6721 cgcgcgaacg ctgctcggtg atcgccacga gcagtccgtc gggatactcc tcgtaggagc
     6781 gtccgagcgc gtagccggcg ttgaccccgg cggccgcgca gcgctcgacg acgctcgaca
     6841 ccgactcaag cccgtccacg tcgaggcgca gcgcgaactc gcgcacgacc ggctggtcgt
     6901 ggagcttgcg cacgccgtcc agcgccgaca gcgtctcgcg cgcgtagtgc gtgcgctgca
     6961 gcatgagctc gccgagctcg acgatcccgc gccgcccgag ccagctcagg tagacgacgc
     7021 cggcgagcgc gttgagcgcc tgtgacgtgc agatgttcga cgtcgcgcgc tcgcggcgga
     7081 tgtgctgctc gcgcgtctgc agcgtgagca cgaagccgcg gcggccgtcg acgtcggtcg
     7141 tctcgccggc gatccggccg ggcatcgagc gcaggtgctt ctccgtcgcg gcgaagaagc
     7201 cgaaggaggg accgccgtag tccaggcggt tgccgagcga ctggccctcg ccgacgcaga
     7261 cgtccgcgcc ctggttgccg ggcgcctcga gcacgccgag cgtcagcggg tcggcgacga
     7321 cgacgacgag cgcgccgtgc tcgtgcgcgg cggcggcgag cgtctgcacc tcctcgaccg
     7381 tgccgag
//
LOCUS       DASVEU010000164         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_311593,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000164 DASVEU010000000
VERSION     DASVEU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311593"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..800
                     /locus_tag="VF225_06560"
     CDS             348..800
                     /locus_tag="VF225_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7144955.1"
                     /translation="MRVYQRETELRTAGGLAVRDITDEVNEAVEASGITEGIACIYSP
                     HTTCCVRVNEFETGFIEDFSELLRRLVPSETYYAHDDWDKRTENICPEDMDFGNGHSH
                     CMAMLLGTAGESVPVREGELCLGTWQRVLFIELDRERDRRWLVQVVGN"
     gene            819..2207
                     /locus_tag="VF225_06565"
     CDS             819..2207
                     /locus_tag="VF225_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7144956.1"
                     /translation="MGAGPNGLAAAITLVQAGRSVRVIEAQETVGGGARTAELTLPGF
                     RHDVCSAIHPLAVASPFFRSLELELDLIEPPAAVAHPLDDGTAAIARRSLADTAASLG
                     SDGDVYRRLLEPLVTGWDELEGEVLKPIAHVPRHPLALARFGLAALRPAKRLAEARFE
                     TEAARALFAGAAAHSSLRLEQPASASFGLVLLMLAHISGWPFPRGGSQAIADALAARL
                     RSLGGEIETGNRVSSLRELPSGKVVLCDVTPRQLLAIAGDVLPDGYRRRLERWRYGPG
                     AFKLDYALDGPIPWRAPEVAEAGTVHLGGTLDEIANSERAAWEGRHAERPFVLLAQQS
                     LFDETRAPSGKHTAWAYCHVPNGSTFDMSERVEAQIERHAPGFRDRVLARASRNTRDL
                     ERENGNLVGGDISGGANTLPQLLARPAPRRIPYSTPLPWLYLCSASTPPGGGVHGMCG
                     HLAAKAALARPR"
BASE COUNT          389 a          832 c          763 g          354 t
ORIGIN      
        1 gagcgccgat gtcgacgtgg ctcccgctcc accagccacg atcgcaaccg ccgccgcgac
       61 cgccaccagc gtgaagatcc ttgtccatcc aaacatgagc cctccccttt acgccatgcc
      121 gcagccagag ccgcagtcca caatccgtag acaatcaagg caccttcgcg gcgccgcaga
      181 cgaccatcgt tagcaccttc cccggcaccg gtcaacccga ggctcgcatc tcggagggag
      241 ttggaccgtc tgccgaagga tggaagcgtc atcccgtcca ggcccggaga accctgcaaa
      301 aacggttccc acttgcgtag cccgccggaa tcctcagaat aggggcggtg agggtctacc
      361 agcgcgagac cgagctccgt accgcgggcg ggttggccgt ccgcgacatc accgacgagg
      421 tcaacgaggc ggtcgaggcg agcggcatca ccgagggcat cgcgtgcatc tactccccgc
      481 acacgacgtg ctgcgtccgg gtcaacgagt tcgaaaccgg cttcatcgag gacttctccg
      541 agctgctccg tcgcctcgtc ccgagcgaga cctactacgc gcacgacgac tgggacaaac
      601 gcaccgagaa catctgcccg gaggacatgg acttcggcaa tgggcactcc cactgcatgg
      661 cgatgctgct cggcacggcg ggcgaatccg tgccggtacg tgagggcgag ctctgcctcg
      721 gcacctggca gcgggtgctc ttcatcgagc tcgaccgcga gcgcgaccgg cgctggctcg
      781 tacaagtagt cggcaactag acgctagatg cggtcgtaat cggagcaggg ccgaacggcc
      841 ttgctgccgc gatcacgctc gtccaagcgg ggcgctcggt tcgcgtcatc gaggcgcagg
      901 agaccgtcgg tggtggggct cgcacagccg agttgacact gccgggattt cgccacgacg
      961 tctgctcggc gattcatccg ttggccgttg cctccccctt cttccgctcg ctggagctag
     1021 agctcgacct gatcgagccg cccgctgcgg tcgcccaccc gctcgacgac gggaccgcgg
     1081 caatcgcgcg gcgatcgctc gcggacacgg cagcgagcct cggcagcgac ggcgatgtgt
     1141 accgacgact gctcgaacct ctcgtcaccg gctgggacga gctcgagggc gaggtgctga
     1201 agccgatcgc gcacgttcct cgccatcctc tcgcgctcgc gcgcttcggt cttgccgcgt
     1261 tgcgtccggc gaagcgcctc gcagaggcgc gcttcgaaac cgaggcggcg cgcgcattgt
     1321 tcgcgggcgc ggccgcacac tcgtcactgc gactcgagca accggcgagc gcgtctttcg
     1381 ggttggtact gctgatgctt gcgcacatct cgggctggcc cttcccacgc ggcggctcgc
     1441 aggcgatcgc ggatgcactc gccgcgcggc tgcgatcgct cggcggagag atcgagaccg
     1501 ggaaccgtgt ctcatcgctc cgcgagctgc cgagcggcaa ggtcgtactg tgcgacgtga
     1561 caccgagaca gctgctcgcg atcgcgggcg acgtgctgcc agacggttac cgccgtcggc
     1621 tcgagcgttg gcgctacggg ccaggcgcgt tcaagctcga ctacgcgctg gacgggccca
     1681 tcccgtggcg tgcgcccgaa gtcgcagaag cgggaaccgt tcatctcggc gggacgctcg
     1741 acgagatcgc gaactcggag cgcgcagcct gggaaggaag gcacgccgaa cggcctttcg
     1801 tcctgctcgc acaacaaagc ctgttcgacg aaacgcgcgc gccgagcggc aagcacacgg
     1861 cgtgggcgta ctgtcacgtc ccgaatggat cgacgttcga catgagcgag cgcgtcgagg
     1921 ctcagatcga acgccacgcg ccgggctttc gcgaccgtgt tctcgcgcgc gcgtcacgca
     1981 atacaagaga cctcgagcgt gagaacggca acctggtggg cggcgacatc tccggaggcg
     2041 cgaacacgct tccacagctg ctggcgcgtc ccgcgcctcg acgaatcccc tactccactc
     2101 ccctcccgtg gctctacctc tgctcagcct cgaccccgcc cggtgggggc gtccacggga
     2161 tgtgcggcca tctcgcggca aaggccgcgc tcgcgcgccc ccgctaggcc tgtgaccgag
     2221 cgcgcgcggg cgcctctcgc cgccgcggcg tgtcccgacc aggcggcaac cccggaacga
     2281 tcggcgcctc gggataggcc tccaccgagt cctgccgccc acgcggctgc ggatagag
//
LOCUS       DASVEU010000165         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_699928,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000165 DASVEU010000000
VERSION     DASVEU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699928"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..712)
                     /locus_tag="VF225_06570"
     CDS             complement(116..712)
                     /locus_tag="VF225_06570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7144957.1"
                     /translation="MPLALLQGLIDLLTGSLWTYPLLFGICAGDALIPAFPSETALIV
                     CGIQAARGQLSLELVIVCAAAGAFTGDNASYAVGRWLGRPAVKRFFMGEVAQGRLDWA
                     RNFLKERGSYVLVVARFIPGGRTATTFTAGLVRLRWMTRFAPYVFVAAILWAVYGGLL
                     GYLGGRLFEDQPIYALLLALGIAALITFGVEAWRRVRK"
     gene            complement(712..1566)
                     /locus_tag="VF225_06575"
     CDS             complement(712..1566)
                     /locus_tag="VF225_06575"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7144958.1"
                     /translation="MTICDVGPRDGLQNEPETLAPVVRAELVDRLAAAGLPRVEAASF
                     VRADAVPQMAGAEEVVAAIHRREGTEYSGLALNEKGYERLAAAGLDRVNFTLAATDSF
                     SRRNANQSVEEAAAAAAGVIKRAAVPVTLTISVAFGCPFEGRVDPGVVAELAARFGAA
                     EVVLADTIGVATPRQVRDLVERTHAPGVHLHNTRNSGYANAVAALEAGATLFDASCGG
                     LGGCPYAPRATGNIATEDLVYLLEGDGIETGVNLDALIETSVWLESLLGRRLEGQVYR
                     AGAWTGTD"
     gene            complement(1580..2065)
                     /locus_tag="VF225_06580"
     CDS             complement(1580..2065)
                     /locus_tag="VF225_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7144959.1"
                     /translation="MSVDVAAARERLRAEKTVPGLLAETARAYVELLDAPACTISRVI
                     GDLLVDLMQHQKSGKPDRLGHGYLISDYPLTRAVIEERRPQTVSQDDPDPDENELKLL
                     RELGYDALLMVAIEGGDGVWGLVEVYGEKGRRFTDEEVELAQDLAVEVGEILKQLERP
                     K"
     gene            complement(2088..2492)
                     /locus_tag="VF225_06585"
     CDS             complement(2088..2492)
                     /locus_tag="VF225_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7144960.1"
                     /translation="MAKLEEKPRKFLDESPNVGVATTLREDGSPHSTIVWVDVEDGKV
                     SFNTARGRAKSKHLERDPRASLLMVDPNNAWRWVAVSGPAELTEEGADAQIDKLAKKY
                     LGKDEYPWRNPEETRVKVLIEPEKVDATGFDE"
BASE COUNT          406 a          898 c          809 g          435 t
ORIGIN      
        1 acgcgcgcgc gtgagcgcct cttcccccca cggataccaa tcgacaggat tgtcggcgtg
       61 ctgcagaagg tacgggctcg tctcttgggc gagacggttc actgaggcac cgtagctact
      121 tgcggacgcg gcgccaggcc tcgacgccga aggtgatcag cgccgcgatt cccaatgcga
      181 gcagcagcgc gtagatcggc tggtcttcga acaggcgccc gccgagatag ccgagcagcc
      241 ccccgtagac cgcccagagg atcgcggcga cgaacacgta cggcgcgaac ctcgtcatcc
      301 agcgtaggcg caccagcccc gcggtaaagg tcgtcgcggt gcgaccgccc gggatgaacc
      361 gcgcgacgac caggacgtag ctgcctcgtt ccttgaggaa gttccgagcc cagtcgagcc
      421 tgccctgggc gacctcaccc atgaagaacc gcttgacggc gggccggccg agccagcggc
      481 cgaccgcata ggacgcgttg tccccggtga acgcgcccgc ggcggcgcag acgatcacca
      541 actcgagcga aagctggccg cgagcggcct ggataccgca gacgatcagg gccgtctcgc
      601 tggggaacgc gggaatcagc gcgtcgccgg cgcagatccc gaagagcagc gggtacgtcc
      661 acagcgatcc ggtcagcagg tcgatcagac cctggaggag cgcaagcggc atcagtcagt
      721 gcccgtccac gcaccggcgc gatagacctg gccttcgagc cggcgtccga gcaagctttc
      781 cagccacact gaagtctcga tgagcgcgtc gagattgacg cccgtctcga tcccgtcgcc
      841 ttccagtaga tagacgagat cctccgtcgc gatgttcccg gttgcgcgtg gtgcatacgg
      901 gcagccgccg agcccgccgc aggacgcgtc gaacagcgtc gcgccggcct cgagcgcggc
      961 gacggcattc gcgtagccgc tgttgcgggt gttgtgcagg tgcactccgg gagcgtgagt
     1021 ccgctcgacg aggtcgcgca cttgccgcgg cgtcgcgaca ccgatggtgt ccgccagcac
     1081 gacttcggcg gcaccgaacc gcgcagccag ctcagcgacc acgcctggat cgacgcggcc
     1141 ttcgaaagga caaccgaaag cgacgctgat cgtcagcgtc acgggcactg cggcacgctt
     1201 gatgacgcct gctgcggccg cggccgcctc ctccacggat tggttcgcgt tccgcctgct
     1261 gaagctgtcg gtcgctgcaa gtgtgaagtt gacccggtcg agaccggctg cggccaagcg
     1321 ctcgtatcct ttttcgttca acgccagacc ggagtactcg gtcccttcgc gacggtggat
     1381 cgccgcgacc acctcttcgg cgcccgccat ctgcggcacg gcgtcggcgc ggacgaagct
     1441 cgcggcctcg acgcgcggca gccctgcggc cgcgagccga tcgacgagct ctgcgcggac
     1501 aaccggcgcg agcgtctccg gctcgttctg gaggccgtct cgcggcccca cgtcgcagat
     1561 cgtcacgcgc acgacgaggc tacttgggcc gctcgagctg cttgaggatc tcccccactt
     1621 cgaccgccag gtcctgcgcg agctcgactt cctcgtcggt gaagcggcgt cccttctcgc
     1681 cgtagacctc gacgagcccc cagacgccgt ctccgccttc gatcgcaacc atgagcagcg
     1741 cgtcgtaacc gagctctcga agcagcttca gctcgttctc gtccgggtcg gggtcgtcct
     1801 gcgacaccgt ctgcggtcgc cgctcctcga tcaccgctcg cgtgagtggg tagtccgaga
     1861 tcagataccc gtgcccgagt cggtccggct tgccgctctt ctgatgctgc atcaggtcga
     1921 cgagcagatc gccgatcacg cgggagatcg tgcaggcagg cgcgtcgagc agttcgacat
     1981 aggcccgggc ggtctccgca agaagccccg ggacggtctt ctcggcacga agccgttctc
     2041 tggcggcggc tacatcgacg ctcacgcttc gatcttacgg agcgccttta ttcgtcgaaa
     2101 cctgtggcgt cgaccttctc gggctcgatc agcaccttga ccctggtctc ttccgggttc
     2161 cgccacggat attcgtcctt accgaggtac ttcttcgcga gcttgtcgat ctgggcgtcg
     2221 gccccctctt ccgtcagctc ggccgggccg ctcaccgcca cccatcgcca cgcgttgttc
     2281 ggatcgacca tcagcaacga ggcccgtggg tcgcgctcga gatgcttcga cttcgcccgt
     2341 ccccgagcgg tattgaagga gaccttccca tcctcgacgt cgacccagac gatcgtcgag
     2401 tggggcgaac cgtcctcccg cagggtggtc gcgactccga cgttgggact ctcgtccagg
     2461 aacttgcggg gcttctcctc gagctttgcc atgcctcacc tccggttgtt ttgaccgaca
     2521 acgacgttag ccgctagctt cttccccg
//
LOCUS       DASVEU010000166        19129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_312754,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000166 DASVEU010000000
VERSION     DASVEU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 19129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19129
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312754"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VF225_06590"
     CDS             complement(<1..909)
                     /locus_tag="VF225_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7144961.1"
                     /translation="MQRSSGTLAAANITKHHAAQLVLSDVTLVVPPRSRIGLVGPNGA
                     GKSTLLRILGGLEEPDAGTVRRLPADLTVGYLPQERDADSAEPLGAYLARRTGVEGAA
                     IEMDQLARRLGEEPQLAERHAGALERFLLLGGGDLDARARTVCAEVGLNAPLAAPLGA
                     LSGGEAARAQLAAVLLARFDVFLLDEPTNDLDFAGLDRLERFVLNLAGSVVVVSHDRD
                     FLDRMVGRVVELDEWTHRATEFAGGWSEYEQARANALRRQAEAYGAYEGEKARLEVQL
                     RRMQQWEERGYGQGRKKKKTKDVKKSI"
     gene            complement(996..1796)
                     /locus_tag="VF225_06595"
     CDS             complement(996..1796)
                     /locus_tag="VF225_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX7144962.1"
                     /translation="MGLTALLTSAMLAVTPASYVASHQQADGGFAEPGSRSAPGLTAW
                     AMLGLRSTRRSTGAAYDYLVANEGELRSATDIELALIAEAATGRISDTLYARFTALGR
                     PDGRIGPAINSTAWGILAYAQVGRPFKPRVSYLLRHQHRSGGWGWIAGAAPDSNDTAA
                     VIQALRTQGVQGRSIARGVAFLRRLQNADGGFELTAGRGSDAQSTAWAIQAFIAARVK
                     APVGAFRYLAALRAGNGSFRYSRKYAATPVWVTSQVLPALARKPFPLR"
     gene            complement(1787..2464)
                     /locus_tag="VF225_06600"
     CDS             complement(1787..2464)
                     /locus_tag="VF225_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX7144963.1"
                     /translation="MSRAALFVAGTAAFAAAVWAAVDRSHDGLSLLLVAFALLAAGVA
                     WRERGPDSAKELALIATLGAAAAAGRVLFAAIPGVQPVTVMTVAAGAALGARSGFAVG
                     ALAAFVSNFFLGQGTWTPWQMLGWGACGVAGALAPRLLRRRVALAAFCFVLGLAFSAF
                     MDVWNWLAFYDEHTWQTFAAVHARGLPFDLAHAIGNVVIALAAGPELRRLLDRYGKRL
                     HTEVVWV"
     gene            complement(2457..3800)
                     /locus_tag="VF225_06605"
     CDS             complement(2457..3800)
                     /locus_tag="VF225_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7144964.1"
                     /translation="MALARADRLTFTYPDAGTPALRDVSLEIEPGEVVAVLGPSGSGK
                     STLLRAFAGLVPHFHGGRFEGRVEIAGLDTRSARPAELAGTVASVFQDPEEQIVLSRV
                     IPEVAFGLENIGTPPADILRRAHEALASVGSEHLADRAVAELSGGELQRVCLASAVAL
                     RPKLLLLDEPTSQLDPGGADAFLEYVVGLGIAVLISDHRPRRVLEHADRVLFLDAGRV
                     LLDAAVPAAEDWLGHNRPVWLGASGNGRVPTQGEPLCRLEGVQFAYRGGLPVVDGVDL
                     ELRRGEIVALGGANGTGKTTIAKLAAGLLAPDAGTAELRGRAAMLLQDPSRYCIRERA
                     DEEVAVGVRGDRRRALAALAELGLSGMASRHPRDLSTGERERLALASVLVTDPDVLVL
                     DEPTRGVDPQRKAALAELLHDGSERRATLVVTHDEEFVRIVADRSITLGRESLLV"
     gene            complement(3791..4477)
                     /locus_tag="VF225_06610"
     CDS             complement(3791..4477)
                     /locus_tag="VF225_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HEX7144965.1"
                     /translation="MKPGAAVALLAALAAAALLAQHLAAVAVVALVLFAVCLRAPAER
                     RRLYLFGTLVSAGALFLLTPFIETIGSHPIWTGPIVPVLGQLDITHEELTSAGLNALR
                     LAAVGLAFAAYALLLDHDALLQAVRFARPAALATRLMPTLERDAAGFVEALRGRGVEV
                     NGVRGRAVLLSPLLASALERGLNLAEAMEARGYGRTGRTSIPRPCWRARDSVVVALAA
                     AIVTAGVLWL"
     gene            complement(4474..5166)
                     /locus_tag="VF225_06615"
     CDS             complement(4474..5166)
                     /locus_tag="VF225_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX7144966.1"
                     /translation="MRRLVLLLAVAVLAGCGGGESGQATLWITRDQGAHVMLAQKVPA
                     GLTAMQALDRSADIKTRYSGRYVQAINGIEGSIDARRDWFYFINGYEADRSAAEYRLH
                     DGDVEWWDFRSWATRMREPVVVGAFPEPFLHGYGGSRRPALVLFSLEVQRSAAQRLAK
                     LIGGRATKGLNPGPTKNMNLLVLRSPGPKPVLHAGGGNAPGDPVVFTFSGDPELLLRR
                     VPFGRFRFQVGP"
     gene            complement(5163..5801)
                     /locus_tag="VF225_06620"
     CDS             complement(5163..5801)
                     /locus_tag="VF225_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX7144967.1"
                     /translation="MSHRLIVVVAALVAALLVVPAALAVKVHVRVEGKERTIFGATAP
                     TIDVTSPRANALPENALDALETASVFGEFYYHVTQSSFGPYVDQVGRYPAVGQTGWVF
                     KVNGASPPVGADQVRLQQGDVVLWYWAQFGVAGGPKTLVLSRVGKSPCYRVQAQDDNG
                     ALTAAIGAVLHVGKRTVKTQGASQTALGCVGPHRGLLVRATLGGAVRSNALP"
     regulatory      complement(5844..5968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6011..6355
                     /locus_tag="VF225_06625"
     CDS             6011..6355
                     /locus_tag="VF225_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7144968.1"
                     /translation="MPRSRLLTVWVPVLVWAGVIFAFSSIPHLSTGLGTWDTILRKGA
                     HITEYAVLGGLLYRALNREALALAAGIAYAATDELHQHFIRGRHASPVDVAIDAVGVA
                     VGMLVWLRLRER"
     gene            6352..6765
                     /locus_tag="VF225_06630"
     CDS             6352..6765
                     /locus_tag="VF225_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144969.1"
                     /translation="MNAVAISLSALGDMEPLWRDWLDDARRRYRVDGDALDQELPNWR
                     ELLERFAEERAPVYFRRDADVSRTLRQLRASGTRIGVFTDAPAELARVALSHLGAERH
                     IEVLESGPGAQKRILAALGDDARVIDTRDELLAVT"
     gene            6771..6992
                     /locus_tag="VF225_06635"
     CDS             6771..6992
                     /locus_tag="VF225_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144970.1"
                     /translation="MERPDVGDRQLDALLERLDRLCNEMQTLNKRLDSNDQLLRIARE
                     LSTLNESLQTLAYAALGQQAPTVRRRRTG"
     gene            complement(6989..8053)
                     /locus_tag="VF225_06640"
     CDS             complement(6989..8053)
                     /locus_tag="VF225_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7144971.1"
                     /translation="MLAPLLAAAALVSPATQEARQELSVRPFARGLSAVTAIASNKAE
                     PRRLYVVEQVGRIRYFVNGKLAGTFLDIRDRVVSGGEQGLLSVAFHPRYARNHRFYVN
                     YTDKSGNTRVVEFRSRNGRGIKSTARQLLFVRQPFANHNGGELQFDSSGRLYVGMGDG
                     GSGGDPGNRAQNMNERLGKLLRIDPLVRGAKWEVVGLGLRNPWRFSFDRANGDLYIGD
                     VGQGNWEEIDYRPRASISTLANYGWKVFEGRARFSNSALGTGQLVTPVYVYSHAESNC
                     SVTGGYVYRGRAVAAAVGRYFFGDYCSGIVWSLRIQNGSAADVRREPFHVSNLTTFGE
                     DAAGELYFGTADGEIFTLAG"
     gene            complement(8111..8773)
                     /locus_tag="VF225_06645"
     CDS             complement(8111..8773)
                     /locus_tag="VF225_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX7144972.1"
                     /translation="MYVLIAGGGKAGANVMRTLLRNRHEATIIEQDRNRFERLESEFE
                     HQAMQGDATELFVLEKAGVKRPPDLVLALTGDDEDNMVITQIAKEKYGVPKVIARVND
                     PRNQPHFDLLGISPTVCATSSILGLVEHEVPEHDLVHLLELRKENLEIVEVQIDKSSP
                     CAGTTVEKLRLPEGARLISVMRDGKAEIAVGKTELKPGDQVLAVLQPGKEDELRQILL
                     KK"
     gene            complement(8774..9205)
                     /locus_tag="VF225_06650"
     CDS             complement(8774..9205)
                     /locus_tag="VF225_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX7144973.1"
                     /translation="MKLIVIGCGRVGSTIAKRFAAEGWDVTAVDENESALSRLGENWT
                     GGFVRGHGMDNAVLREAGIEDADAVVVSTDGDNTNLVIGQVAQKRFGIECTVVRILDP
                     ARADFYRERGLRTISPTSVAIEALSDAVRSCEIPQKQEVGV"
     gene            complement(9397..11289)
                     /locus_tag="VF225_06655"
     CDS             complement(9397..11289)
                     /locus_tag="VF225_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7144974.1"
                     /translation="MARKLPRLQRVLDAPALFSIAYGEIASSIYFALGIIALHALGFT
                     PLVLLATGLLFLIVSLSYAEGTAAIRETGGAATFVRIAFNDFWGFVTGWVLFLDYLIV
                     IALSALFLPHYVAGAFFTKVHRPWDVVAGCGVIAGVAAIRLVHRTRLHRWALIVALVD
                     LATQLLIVALGMALLFSPHALTAGTSLGTSPTWHSIAFAIPLAMLAYTGLETVANLAE
                     ETRRPGRDLPRSLFGAIGLVVVLYVVIAVVGLSAFPPKHGTLLGSNWIRAPLVGIVTQ
                     IREHVPNVIGAPLQVFVGLSGALILLTAATTSISGFGRLAYSLGEHGQLPRRFGMLRP
                     RTLVAPESILAAALISIALLVGTAALAHPVLFLASLFSFGVLLAFTAAQLAVIKLRFS
                     EPNRRRPYRVPFTVGRVPVPSVVGALLTFTVWLIAIATHQGARLAGPAWLGVGLVVYI
                     VVRRSRGEGLLERVVSADEHRGLAEAEFSRILVPMKLGDIGEEMIATAVKLAQERGVS
                     VEALHVIRVPMELPLDAELLEEEGRAEASLAEAKALGADHGVQVVGKTVRARSIGQAI
                     VQEAEDSGADLIVLGSAPRWRRQSRFFSPTVDYVLKKAPNEVLIVAFPQGALEGDGSP
                     SPQGGR"
     gene            complement(11300..11476)
                     /locus_tag="VF225_06660"
     CDS             complement(11300..11476)
                     /locus_tag="VF225_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144975.1"
                     /translation="MNAAYRSLVMIFAFLFMGIGIALVAVTAVNGGGAFGYVLGALFF
                     ALGVGRLYLLRRRR"
     gene            complement(11473..13383)
                     /locus_tag="VF225_06665"
     CDS             complement(11473..13383)
                     /locus_tag="VF225_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7144976.1"
                     /translation="MSATPTPRRSRRRRQQTLERVLGTPALFATAYGNVGSSIYYALG
                     LVAGIALGLTPLVFVISGLIFAATAATYAEGTVRFPEAGGSSSFARHAFNELVSFGAA
                     WAQMLNYIITIAISAFFVPHYLSIFWSPLRSNPWDIVGGAIVVVLLVLLNIVGIREAA
                     RLNILLAVLDFGTQLLLVVVGFVLIFDHHVVFNTNLHWGVAPTWGQFFLAIPIAMIAY
                     TGIETVSNLAEEARDPPRDIPRSITWVAGAVFAIYFTLPLIALSGLPVEKTAHGYQTL
                     LGVGPDQGGFKNDPVLGLVEHLGLHGQVLQASKIYVGILAATILLIATNAGVIGASRI
                     TYAMASYRQLPEVFRRLHPKFKTPWLSLLVFAGLLSIVVLLPGRTDFLGNMYAFGAML
                     SFTIAHVSVIALRMSTRDDPEAFKVRPSLRIRGVDWPLFAIFGGLGTGIAWLVVVIQK
                     PGPRWAGLGWLLIGLIGYVVYRRRVLHEPLTATLHAPMIIGPAIALEYRNILVPVKPG
                     RASEEAIDLACRLAADRGASIAALSVVVVPLDQPLDTRLEDEERMADEALDIAAAIAE
                     LYGVKFTERLVRARHAGRAIVDEATRRQSEIIVMGAPRGGRGRGVFSETVDFVLKHAP
                     SRVMVVAGRIAA"
     gene            complement(13656..15668)
                     /locus_tag="VF225_06670"
     CDS             complement(13656..15668)
                     /locus_tag="VF225_06670"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7144977.1"
                     /translation="MLFALVCAGVAIAYGIWLTFWLLGRPDGNERMREIAKAVQEGAA
                     AYLRRQYTTIAAVSIVPFLLLGFYNKLGWGTAIGFLVGAILSAGAGFIGMNVAVRANV
                     RTAEAARSGLKPALNVAFRAGSVTGMMVVGLALLGVAGYYWALTGWLGNSADSAIHDL
                     IGLAFGGSLISVFARLGGGIYTKAADVGADLVGKIEAGIPEDDPRNPAVIADNVGDNV
                     GDCAGMAADLFETYAVTAVAVMLLGTQFPSSGLALYPLALGGISILASVIGTFFARVG
                     RTGNIMNALYKAVLVATVLSAIGFIPVTMAYDGGKFSFTDLYCASLVGLVVTFLLVAI
                     TEYYTGTRWGPVKSISSASQTGHATNIIEGLAVGMKATALPVVVIAAGILIAHHFAGL
                     YGIGIAVMAQLSMTGLIVALDAYGPVTDNAGGIAEMANLDESVRAITDPLDAVGNTTK
                     AVTKGYAIGSAALAALVLFDSYTTGLADEGLTSTFSLSDAWVIAGLFIGGLMPFLFAS
                     LAMQAVGRVGGEVVTEVRRQFRENPGIMEGTSRPEYGRAIDIVTSSAIKAMLLPALIP
                     ILFPIIVGIINARMLGGLLIGTIVTGLFLAIAMTSGGGAWDNAKKLIEDGAFGGKGSE
                     AHAASVTGDTVGDPYKDTAGPAINPMIKIANIVAILIIPLIVSIHG"
     gene            complement(15792..15974)
                     /locus_tag="VF225_06675"
     CDS             complement(15792..15974)
                     /locus_tag="VF225_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144978.1"
                     /translation="MAIDVAGVIHCGCGQVLWGEDEEQLLQDADLHVRKAHPELVGTL
                     SPLELARPAVTDEAAV"
     gene            complement(16018..16317)
                     /locus_tag="VF225_06680"
     CDS             complement(16018..16317)
                     /locus_tag="VF225_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144979.1"
                     /translation="MTESGPPSDDELLKQLEEELKTLEVSDVLVQTLYTVSSLGYRRL
                     SEEGRDLDQARLGIEALRALLPVLEGAAGAELVRDFKQVTANLQLAYADAAKGEG"
     gene            complement(16314..16673)
                     /locus_tag="VF225_06685"
     CDS             complement(16314..16673)
                     /locus_tag="VF225_06685"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144980.1"
                     /translation="MGEAVEGVGGVFFRASDPVALRAWYAEHLGIAMEDYGTTFTSKD
                     GDQTVWAPFPADTRYFGPGGQQLMVNFRVRDLDAMLAQLRAAGVDVEDRVEEMEYGRF
                     AWASDVEGNRFELWEPS"
     gene            complement(16666..17826)
                     /locus_tag="VF225_06690"
     CDS             complement(16666..17826)
                     /locus_tag="VF225_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX7144981.1"
                     /translation="MGDQQRLAPEAQIGLAREAFENGDDFTIAVEEEFALLDPDTLEL
                     TSRFEELKAGAAGTELDEHLVGELIASEIEVKTGRCENFSEAAERMAERRAQLGELAD
                     RFGVGLASTGTHPWSRWQDQRIIDTPHYRRNNELLQYVVWRNNSFGIHAHIGIRGADR
                     AVAVCNAFRNVLPELLAVSASSPFLEDCVTGLHSARTEIFTKLFPRCGVPDAFDDWTA
                     YEEYVRFLFATRSIDEHTQIWWSVRPHLAFPTVEVRICDAQPDLAQAQSLAALIYALA
                     TRFARALDEGEALPDLPHRLIEENMWRAIRYGLTDGMIDFDRGEVVPARARVEALIEW
                     VLPVAEEIGAAPYLAVPAANAAERQYERVAELGSHRDVFAELVRRPRERVGG"
     gene            complement(17856..>19129)
                     /locus_tag="VF225_06695"
     CDS             complement(17856..>19129)
                     /locus_tag="VF225_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7144982.1"
                     /translation="IAPGGGTTRILLWHGYLLGGTGSNVYTRSLAREWSRAGHDVLVI
                     CQEPHPERYDLGGAEVFRPNIGGLLPVFVLDRYEGVEAKLLQDFTIDERARYVALNAT
                     ALCAQMPADLVFANHVLLGGAVGYASGLPFAVKAHGSELEYSMRGNEELAAWGRECLA
                     LAEAVFVGSAHIRTVLEEVVGHVDRVHEVPPGVDVEEFRPSRSREESLSRLLEEARAD
                     ADNPGNSDERQPDEGNATRLEQFFDTDEPTVVYFGKLLYNKGVHLLFEALNSLDARAL
                     IVGFGDYRQELESIAPPGTLFTGPLEHRHLVHLLPLADVTVVPSIFPEAFGMVAAEAA
                     AAGSPPLVARHSGLAEIAEGLEAEYPAEYRHLASFANGDAADLAAKLDELLALPPAAR
                     AELGAAARRAAVERWSWTSVATRLLEPIRGG"
BASE COUNT         3284 a         6545 c         6391 g         2909 t
ORIGIN      
        1 gatcgacttc ttcacatcct tggtcttctt cttcttgcga ccctgcccgt acccccgctc
       61 ttcccactgc tgcatccggc gcagctgcac ctcgaggcgc gccttctcgc cttcgtaggc
      121 gccgtaagcc tccgcctggc ggcgcaaggc attggcgcgt gcctgctcgt actcgctcca
      181 ccctccggcg aactcggttg ctcgatgtgt ccattcgtcg agctccacga cgcgcccgac
      241 catgcggtcg aggaagtcgc ggtcgtgcga cacgacgacg accgaccccg cgagattcag
      301 gacgaacctc tccaggcggt cgagccccgc aaaatcgaga tcgttcgtcg gctcgtcgag
      361 cagaaagacg tcgaaacgcg caagcagcac ggcagccagc tgggcgcggg cggcctcgcc
      421 tcccgagagc gcgccgagcg gcgcagcaag aggcgcattc agacccacct ccgcacacac
      481 cgtgcgcgca cgggcgtcga gatcgccgcc gccgagcaac agaaaccgct cgagtgcccc
      541 ggcgtgacgc tcggccagtt gcggctcttc gccgagccgt cgcgcaagct gatccatctc
      601 gatcgctgcc ccttcgacgc ccgtgcggcg tgcgaggtac gccccgagcg gctcggcgga
      661 atcggcatcg cgctcctgag gcaggtagcc cacagtgaga tcagcgggca ggcggcgcac
      721 cgtgccggcg tcgggctctt cgagcccgcc gaggatgcgc aggagcgtcg acttccctgc
      781 gccgttcggt ccgacgaggc cgatgcgact gcgcggcggc acgacgagcg tgacgtcgct
      841 caggacgagc tgtgctgcgt ggtgcttcgt gatgttggct gcggcgagag tgccgctcga
      901 gcgttgcaag agaggacctc ctcggaaggc ggacggaaaa gggtgtgtgt ctcgcctagg
      961 ccctcatagg acggcgcaga gcgccgctcg cgatgctagc gcagcgggaa aggtttccgt
     1021 gccagggcgg gcagcacctg cgacgtcacc cagaccggcg tggctgcgta tttgcgcgag
     1081 tagcgaaagc tgccgttccc ggcccgcagc gcggcgagat agcgaaacgc tccgaccgga
     1141 gccttcaccc gcgcggcgat gaaagcctga atcgcccagg ccgtcgactg tgcatccgac
     1201 cccctccccg ctgtgagctc gaagcctccg tcggcgttct gcaggcgtcg gagaaacgcg
     1261 actccccgcg cgatcgacct tccttggaca ccctgcgtac ggagcgcctg gatgaccgcc
     1321 gcagtgtcgt tggaatccgg tgcggctccc gcgatccagc cccatcctcc cgaccgatgc
     1381 tgatggcgaa gcaggtagga gacgcgcggc ttgaacgggc gccccacctg tgcgtaggcg
     1441 aggattcccc aggcggtcga gttgatcgcg ggcccgatgc ggccgtccgg ccgcccgagc
     1501 gcagtgaagc gggcgtacag agtgtccgag atgcgtcctg tcgcagcttc ggcgatcagc
     1561 gcgagctcga tgtccgtcgc cgaccgcaac tcgccctcgt tcgcgacgag atagtcatag
     1621 gccgccccgg ttgaccgtct tgtcgaccga agacccagca tcgcccacgc cgtgaggccc
     1681 ggtgccgaac gcgaaccggg ctcggcgaag cctccgtcgg cctgttgatg cgaagccacg
     1741 tacgacgcag gtgtgaccgc aagcatcgca ctggtcagga gggcggttag acccacacga
     1801 cctcggtgtg cagacgtttc ccgtaccgat cgagcagccg gcgtaactcc ggcccggcgg
     1861 caagcgcgat gacgacgttt ccgatcgcat gggcgaggtc gaacgggagg ccgcgcgcgt
     1921 gcacggcggc gaacgtctgc caggtgtgct cgtcgtagaa cgcgagccag ttccagacgt
     1981 ccatgaacgc gctgaacgcc aggccgagga cgaagcagaa cgccgcaagt gcgacccggc
     2041 gtcgcagaag cctcggcgcc agcgctccgg ctacgccgca agctccccag ccgagcatct
     2101 gccacggtgt ccacgtgccc tggccgagga agaaattcga gacgaacgcc gccaaggccc
     2161 cgaccgcgaa gccgctacgt gctcctaacg cggcgcccgc cgccacggtc atcaccgtca
     2221 ccggctgcac gcccggaatg gctgcaaaga ggacgcgccc ggcggcggcg gccgcgccca
     2281 gcgtcgcgat caatgccagc tccttggcag agtccggccc acgttcgcgc caggcgacgc
     2341 cggctgcaag caacgcgaac gcgaccagca gcagcgacag gccgtcatgt gagcggtcga
     2401 cggctgccca caccgcagcg gcgaaggcgg cggtacccgc gacgaagagc gcggctctag
     2461 acaaggagtg actcccgccc gagtgtgatc gaccggtcgg ccacgatgcg cacgaactct
     2521 tcgtcgtgcg tgacgacgag tgttgcacgg cgctcggagc cgtcgtgcag caattcggcc
     2581 agcgccgcct tgcgctgggg atcgacaccg cgggtcggct cgtccagcac gaggacgtct
     2641 gggtcggtca cgagcaccga ggcgagggcg agccgctcgc gctccccggt cgagagatcg
     2701 cgcggatgac ggctcgccat tcccgagagg ccgagttccg caagcgcagc gagcgcacgg
     2761 cgacggtccc ctcgcacacc gaccgcgacc tcctcgtccg cgcgctcacg gatgcagtac
     2821 cggctcggat cctggaggag catcgccgcg cggcctcgga gctcggccgt gcctgcgtcg
     2881 ggtgccagca ggcccgccgc gagcttcgcg atcgtcgtct tgcccgtgcc gttcgcacct
     2941 cccagcgcca cgatctcgcc gcgacgcaac tcgaggtcga caccgtcgac gacgggaaga
     3001 cccccgcggt aggcgaactg aacgccctcg agacggcaca gcggctcgcc ttgcgtcggg
     3061 acgcgtccgt tgcccgacgc tccgagccag accggccggt tgtgtcccag ccagtcctct
     3121 gcggcgggca ccgcggcgtc aagaagcact ctccccgcat cgaggaagag gacgcgatcc
     3181 gcatgctcga gaacccgccg gggacggtga tcggagatca gcacggcgat accgagcccg
     3241 acgacatact cgaggaacgc gtcggcgcca ccgggatcga gctgggaagt cggctcgtcg
     3301 agcaagagca gcttcggccg cagcgcgacg gcagaggcga ggcagacacg ttgcagctct
     3361 cctcccgaga gctcggcaac ggcgcgatca gcgaggtgct cggagccgac cgaagcgagc
     3421 gcctcgtgcg cgcgacggag gatgtccgcc ggaggggtac caatattctc gagcccgaag
     3481 gcaacctcgg gaatgacgcg cgaaaggacg atctgctcct ccgggtcctg gaacacactg
     3541 gcgaccgtcc cggcgagctc ggccgggcgc gcggacctcg tgtcgaggcc ggcgatctcg
     3601 acgcggcctt cgaagcgccc gccgtgaaag tgcggaacga gccctgcgaa tgcgcgcagc
     3661 aacgtcgact tgcccgaacc ggacggaccg aggacggcga cgacctctcc cggttcgatc
     3721 tccagtgaga catcacggag ggccggcgtg ccggcgtcgg ggtaggtgaa cgtgagtcga
     3781 tcggcgcgcg ctagagccac agcactcccg cagtcacgat cgccgcggcc agggcgacga
     3841 cgacgctgtc tcgtgcgcgc cagcaaggtc gcgggatgct cgttcgcccg gtcctcccgt
     3901 atccccgcgc ctccatggct tccgcgaggt tgaggccgcg ctcgagcgcg ctggcgagca
     3961 gcggggacag caacacagcg cgcccacgga cgccgttcac ctcgacgccc cgcccgcgca
     4021 acgcctcgac gaaacccgcc gcgtcacgct ccagcgtggg catcaacctc gttgcgagag
     4081 cggcggggcg agcaaaccga accgcttgca gaagcgcgtc gtgatccaac aagagcgcgt
     4141 atgccgcgaa cgcgagtccg acggcggcca gacgaagagc gttcagcccc gcgctggtga
     4201 gctcttcgtg cgtgatgtcg agctgaccga gcaccggcac gatcgggccg gtccagatcg
     4261 gatgcgatcc gatcgtctcg ataaacgggg tcagcaagaa gagtgcgccg gccgagacga
     4321 gtgtcccgaa cagatacaag cgcctccgct ctgcgggtgc ccgcagacag acggcgaaca
     4381 gcacgagagc gactactgcc accgcggcga ggtgctgagc gagcaatgct gccgccgcga
     4441 gcgcggcgag caaagcaact gcggcgccgg gcttcacggg cccacctgga agcgaaaccg
     4501 gccgaacggg actctgcgga gcagcagctc cgggtcgccg ctgaaggtga acacgacggg
     4561 atctccggga gcgttgccgc cgccggcgtg cagcacaggc ttcggtcctg gggaccggag
     4621 cacgagcagg ttcatgttct tcgtcgggcc cgggttcagg cccttcgttg ctcgaccgcc
     4681 gatgagcttt gccagtcgtt gggcggcgga acgttgtacc tcgagcgaga acaacaccag
     4741 ggcgggccgc cgactgcctc cgtagccgtg aaggaacggt tccggaaacg ctccgaccac
     4801 cacgggctcg cgcattcttg tcgcccacga gcgaaagtcc caccactcga cgtccccgtc
     4861 gtggagccgg tactcggcgg cgctgcggtc ggcctcgtag ccgttgatga agtagaacca
     4921 atcgcgtcga gcgtcgatcg agccctcgat cccgttgatc gcctggacgt aacgccccga
     4981 ataacgggtc ttgatgtccg cgctgcgatc cagagcttgc attgcggtca gtcccgccgg
     5041 caccttctgc gcgagcatca cgtgcgcgcc ctggtcacgt gtgatccaga gggtcgcctg
     5101 cccactctcc cctccgccgc agcctgcgag gaccgcgacg gcgaggagaa ggacaagacg
     5161 tctcacggca gcgcattcga gcggacggct ccgccgagcg tcgcgcgcac gagcaagcct
     5221 cgatgcgggc cgacgcaccc gagggcggtc tggctggccc cctgggtctt gacggtgcgc
     5281 ttgcccacat gaagaacagc gccgatcgct gcggtcagcg cgccgttgtc gtcctgggcc
     5341 tggacccggt agcacggtga cttgccgacc cgagaaagaa cgagcgtctt cggaccgccg
     5401 gcaacgccga actgggccca gtaccagagg acgacgtctc cttgctgcaa gcgcacctgg
     5461 tccgctccga ccggaggcga cgcgccgttc accttgaata cccatcccgt ctgaccgaca
     5521 gcgggataac ggccgacctg gtccacgtaa gggccgaagc tgctctgcgt gacgtggtaa
     5581 tagaactccc cgaaaacgct cgccgtttcg agtgcgtcca gcgcgttctc gggcaacgca
     5641 ttcgcgcgcg gcgaggtgac gtcgatcgtc ggagcggtcg ccccgaagat cgttcgctcc
     5701 tttccttcca cccggacgtg caccttgacc gcgagagctg ccggcacgac gagcagcgca
     5761 gcgacgagag ccgccacgac cacgatgagt cggtgagaca cgaatcctcc cttccgcgag
     5821 gggttcgact tagccggcgg cggaggtctt ctgactcggg gctccctccc tcgccgcctt
     5881 cccagccccg tggggccagt ggcatgtggc gcgggagcgt ctccccttac agcggcggga
     5941 ccgttccgga cttgcaccgg attcccttgc cgctcgccgg cttgtggccg gagactagca
     6001 tcgggcgccc atgcctagat cgcgactcct cacggtctgg gttcccgttc tcgtctgggc
     6061 gggcgtcatc ttcgcgttct cctcgatccc tcacctctcg accgggcttg gaacgtggga
     6121 cacgatcctc cgcaagggtg cgcacatcac ggagtacgcc gtcctcggcg ggttgctgta
     6181 ccgcgcgctc aaccgagaag cgctcgcact cgcggccggc attgcgtacg cggccaccga
     6241 cgagctgcac cagcacttca tccgcggccg tcacgcatcg cccgtcgacg tcgcaatcga
     6301 cgccgtcggg gtcgcggttg ggatgctcgt ctggctgcgg ttgcgcgaac gatgaatgcg
     6361 gtagcgatca gcctctcggc gctcggtgac atggagccgc tctggcgcga ctggctcgac
     6421 gatgcgcgcc gccgctaccg ggtcgacggc gacgcgttgg atcaggagct gccaaattgg
     6481 cgcgagctgc tggagcgctt cgcggaggaa cgcgcacctg tctacttccg gcgcgacgcc
     6541 gacgtcagcc gcacgctgcg gcagctgcgc gcaagcggca cgcgcatcgg cgtcttcacg
     6601 gacgcgcctg ccgagctggc ccgagtcgcc ctgtcgcatc ttggcgccga gcgtcatatc
     6661 gaggtgctgg agagcggccc cggtgcgcag aagcggatcc tggcggctct tggcgacgac
     6721 gcgcgggtga tcgacacccg ggacgagctg ctcgccgtaa catgaccccg atggagcgcc
     6781 cagacgtcgg cgaccgccag ctcgatgctc tcctcgagcg gctggaccgc ctctgcaacg
     6841 agatgcagac gctcaacaag cggctcgact ccaacgatca gctgctccgg atcgcacgcg
     6901 agctgtcgac gctcaacgag tctctgcaga cgctggccta tgcggcgctc ggccagcagg
     6961 cgccgacggt tcgccggcgc cgaacgggct agccggccag ggtgaagatt tccccgtctg
     7021 cggtgccgaa atagagctcg ccggcagcgt cctcgccgaa cgtcgtcagg ttggagacgt
     7081 gaaacggctc gcgacgaaca tcggctgcgc tgccgttctg tatgcgcagg ctccagacga
     7141 ttccggagca gtagtcgccg aagaagtagc ggccgactgc cgcggccacg gctcgaccgc
     7201 gatacacgta gccgcccgta accgagcagt tgctttccgc atggctgtac acgtacacgg
     7261 gcgtcactag ctggcctgtg ccgagagcgg agttcgaaaa gcgggcgcgc ccctcgaaga
     7321 ccttccagcc gtagttggca agcgtcgaga tcgatgcccg cggtcggtag tcgatctctt
     7381 cccagttgcc ctggccgacg tcaccgatgt agagatcgcc gttcgcacgg tcgaacgaga
     7441 agcgccaggg attgcgcagg ccgagcccga cgacctccca ctttgcgccg cggacgagcg
     7501 ggtcgatccg cagcagcttg ccgagccgct cgttcatgtt ctgggcgcga ttgcccgggt
     7561 cgccaccgga ccctccgtca cccatgccga cgtagagccg cccgctggaa tcgaactgga
     7621 gctcgccgcc gttgtgattc gcgaagggct ggcggacgaa caacagctgg cgcgcggtcg
     7681 acttgatccc gcgtccattg cgcgagcgga actccacgac gcgcgtattg ccactcttgt
     7741 cggtgtagtt cacgtagaaa cggtggttgc gcgcgtaccg aggatgaaac gccaccgaca
     7801 gcagaccctg ctcgccaccg ctgaccacgc ggtcacggat gtcgaggaac gtgccggcca
     7861 gcttgccgtt gacgaagtag cggatcctgc cgacctgctc gaccacgtag aggcggcgcg
     7921 gctccgcctt gttcgaagcg atcgccgtga ccgcagagag gccgcgcgca aacgggcgga
     7981 ccgacagctc ctgccgggcc tcctgcgtcg cgggcgagac gagggccgcg gctgcgagga
     8041 gaggagcgag cactcgcccc aaactacccg cgaacagccg cggcggatcg cttcttaacg
     8101 aaggacttat ctacttctta agcaagatct ggcggagctc gtcttccttg ccgggctgga
     8161 gcacggcgag cacctgatcc cccggcttga gctcggtctt gccgaccgcg atctcggcct
     8221 tgccgtcgcg catgacggag atcagccgcg cgccctcggg cagccgcagc ttctcgacgg
     8281 tcgtgcccgc gcacggagag gacttgtcga tctgcacctc gacgatctcg agattctcct
     8341 tgcggagctc gagcagatgc acgagatcgt gctccggaac ctcgtgctcg acgaggccga
     8401 ggatgctgga ggtcgcgcag acggtgggcg agataccgag caggtcgaag tgcggctggt
     8461 tgcgcggatc gttgacgcgc gcgatcacct tcggcacccc gtacttctcc ttggcgatct
     8521 gcgtgatcac catgttgtcc tcgtcgtcgc cggtcaaggc gaggacgaga tcgggcggtc
     8581 tcttcactcc ggccttctcg aggacgaaga gctcggtcgc atctccttgc atggcctgat
     8641 gctcgaactc cgactccagc cgctcgaagc ggttgcggtc ctgctcgatg atcgtcgcct
     8701 cgtggcggtt gcgcagcagc gtgcgcatga cgttggcgcc ggccttcccg ccccctgcaa
     8761 tcaacacgta catctagaca cctacctcct gcttctgtgg gatctcgcat gagcgcaccg
     8821 cgtcgctgag cgcttcgatg gcgacggacg tcggggaaat cgtgcgcaag cctcgctcgc
     8881 ggtagaagtc cgcgcgggcc ggatccagga tccggacgac cgtgcactcg atgccgaacc
     8941 tcttctgggc gacctggccg atcacgaggt tcgtattgtc gccgtcggtc gagacgacga
     9001 cggcgtcggc gtcctcgatc ccagcctcgc gcaggacggc gttgtccatg ccgtggcccc
     9061 ggacgaaccc cccggtccaa ttttctccaa ggcggctgag ggctgactcg ttctcgtcta
     9121 ccgcggtcac gtcccaccct tcggcggcga accgctttgc gatcgtcgag cccacccggc
     9181 cgcagccgat aacgatgagc ttcacgtact cagcgtagct cggcggtcaa ccccgaggct
     9241 gctcgccgtc cctttcgaga gctcccgacg gcgtcgcttg cgatgccgtt caacggccgc
     9301 cctgtggact cggcgaaccg tccccttcga gcgctccttg acggcgtcgc ttgcgatgcc
     9361 gtttagaggc cgcgcgggct ttgcccgtgc ggccgttcaa cgcccgccct gtggactcgg
     9421 cgaaccgtcc ccttcgagcg ctccttgcgg gaacgcgacg atcaggacct cgttcggagc
     9481 cttcttgagg acgtagtcga cggttggcga gaagaaccgg gactgcctgc gccagcgggg
     9541 cgcggacccg agcacgatca ggtcggcgcc tgagtcctct gcctcctgga cgatcgcctg
     9601 tccgatcgac cgggcccgga cggttttccc gaccacctgg acgccgtggt ctgccccgag
     9661 cgccttcgcc tcggcgagcg aggcttcggc ccgcccttcc tcttcgagca gctcggcgtc
     9721 gagaggaagc tccatcggaa cgcggatcac gtggagagcc tccaccgaca cccctcgctc
     9781 ctgggcgagc ttcaccgcgg tggcaatcat ctcttcgccg atgtcgccca acttcatcgg
     9841 aacgaggatg cgcgagaact ctgcctcggc gaggccgcga tgctcgtccg ccgacacgac
     9901 ccgctccagc aggccctctc cgcgcgacct gcggacgacg atgtacacga cgaggccgac
     9961 gccgagccag gccggtccgg cgaggcgcgc gccctggtgc gtggcgatgg cgatcagcca
    10021 gaccgtgaac gtgagcaagg ccccgacgac cgacggcacg ggcacgcggc cgaccgtgaa
    10081 cggcacgcga tagggacgcc tgcggttcgg ctcgctgaag cgcagtttga tgacggcgag
    10141 ctgtgcggcg gtgaacgcga ggagcacgcc gaagctgaag aggctcgcga ggaagagcac
    10201 gggatgcgcg agcgctgctg ttccgacgag cagcgcaatc gagatcaaag ccgcagcgag
    10261 aatcgactcc ggcgcgacga gcgtgcgggg cctgagcatt ccgaaccggc gcggcaactg
    10321 gccgtgctcc cccagcgaat aggccaggcg accgaaaccg gagatggagg tggtcgcggc
    10381 ggtgaggagg atcagcgccc ccgacagccc cacgaagacc tgtagcggcg caccgatcac
    10441 gttcggcacg tgctcgcgga tctgagtgac gattccgacg agcggcgcgc ggatccagtt
    10501 gctgccgagc agcgtcccgt gcttcggcgg aaatgcggag aggccgacga ccgcgatcac
    10561 gacgtagagg acgacgacca ggccgatggc gccgaagagg ctccgcggca ggtcgcggcc
    10621 ggggcggcgt gtctcctcgg cgaggttcgc gacggtctcg agcccggtgt aagcgagcat
    10681 cgcgagcggg atcgcgaacg cgatcgagtg ccacgtgggc gacgtgccca gcgacgtccc
    10741 ggcggtgagc gcgtgcggcg agaacagcag tgccatgccc agcgccacga tcagaagctg
    10801 ggttgcgaga tcgacgagcg cgacgatcag ggcccaccgg tgcaggcgtg tgcggtgcac
    10861 gagcctgatc gctgcgaccc ccgcgatcac cccgcagccg gcaacgacat cccaggggcg
    10921 gtgcaccttg gtgaagaaag cgcccgccac gtagtggggc aggaagaggg ccgagagcgc
    10981 gatgacgatc aggtagtcca ggaagagcac ccagccggtg acgaagcccc agaagtcatt
    11041 gaacgcgatg cgaacgaacg tcgccgcgcc gccggtctca cgaatcgccg cggttccctc
    11101 cgcgtaggag agggatacga tcaggaagag caggccggtg gcaagcagca cgagcggcgt
    11161 gaagccgagt gcgtgcagcg cgatgatgcc gagcgcgaag tagatcgacg aggcgatctc
    11221 gccgtaggcg atcgagaaga gcgccggagc atcgagaacc cgctgtagcc ggggcagctt
    11281 tcgggccatg gatttgaggt tagcgccgcc tgcggagcag gtagaggcgg ccgacgccga
    11341 gggcgaagaa cagtgcgccc agcacgtacc cgaatgcccc gccgccgttg accgccgtga
    11401 cggcgacgag cgcgatcccg atgcccatga agaggaacgc gaagatcatc acgagcgagc
    11461 gataggcggc gttcacgccg ctatcctccc tgcgacgacc atcaccctgc tgggcgcgtg
    11521 cttcagcacg aaatcgacgg tttcgctgaa gacaccacgc cctctccccc cgcgcggagc
    11581 gcccatgacg atgatctcac tctggcggcg cgtcgcctcg tcgacgatcg ctcgcccggc
    11641 gtgacgggcc ctgacgagcc gctcggtgaa cttcactccg tagagctctg cgatcgccgc
    11701 cgcgatgtcg agagcctcgt cggccatgcg ctcctcgtcc tcgagccgcg tgtccaacgg
    11761 ctggtcgagc ggcacgacga cgacgctgag agcggcgatg gaggcgccgc ggtcagcggc
    11821 caggcggcag gcgaggtcga tcgcttcctc cgaggcccga cccggcttta ccggcacgag
    11881 gatgttcctg tactcgagcg cgatcgcagg accgatgatc atcggagcat ggagggtcgc
    11941 cgtgagtggc tcgtgcagca ccctgcggcg gtacacgacg tagccgatga gcccgatcag
    12001 gagccacccc agcccggccc aacgcgggcc gggcttctgg atgacgacga cgagccaggc
    12061 gatcccggtg ccgagcccgc cgaagatcgc gaagagcggc cagtcgacgc cccggatccg
    12121 caggctcggg cggaccttga acgcctccgg atcgtccctg gtactcatac gcagcgcgat
    12181 cacggacacg tgcgcgatcg tgaacgacag cattgcgccg aacgcgtaca tgttgccgag
    12241 gaaatccgtc cggccgggca ggagcacaac gatggagagc agaccggcga agaccaacaa
    12301 cgacaaccag ggagtcttga acttcggatg cagccggcgg aagacctcgg gcagctgcct
    12361 gtaggacgcc atggcgtacg tgatgcgcga cgccccgatc acaccggcgt tggtcgcgat
    12421 caaaaggatc gtcgcggcga ggatcccgac gtagatcttc gaggcctgca ggacctgacc
    12481 gtgcaggccc aggtgctcga cgaggccgag caccggatcg tttttgaagc cgccctggtc
    12541 cgggccgacg ccgagcagcg tctggtaccc gtgtgcggtc ttctccaccg gcagccctga
    12601 cagggcgatc agcggcagcg tgaagtagat cgcgaatacg gcgccggcga cccacgtgat
    12661 cgagcgcggg atgtcgcgcg gcgggtcgcg tgcctcctcc gcaagattcg agacggtctc
    12721 gatccccgtg tacgcgatca tcgcgatggg aatggcgagg aagaactgcc cccacgtggg
    12781 tgccacaccc cagtggaggt tggtgttgaa gaccacgtgg tgatcgaaga tcagcacgaa
    12841 gccaacgacg acgagcagta actgggtgcc gaagtcgagg acggcgagaa ggatgttcag
    12901 gcgggcggcc tccctgatcc cgacgatgtt cagcaggacg agcaggacga ccacgatggc
    12961 gccgccgacg atgtcccagg ggttcgaccg cagcgggctc cagaagatcg agaggtagtg
    13021 cgggacgaag aacgccgaga tggcgatcgt gatgatgtag ttgagcatct gcgcccacgc
    13081 ggcaccgaag gagaccagct cgttgaaggc gtgacgggcg aagctcgagg aaccgcccgc
    13141 ctcgggaaag cgaacggtgc cctctgcata tgtcgcggcc gtggcggcaa agatgagccc
    13201 gctgatgacg aagaccagcg gcgtcagccc gagcgcgatc ccggcaacga gcccgagcgc
    13261 gtagtagatc gaggagccga cgttgccgta ggccgtggcg aacagcgccg gagtcccgag
    13321 gacccgctcg agcgtctgct ggcgccgccg ccggctccgc cgcggtgtcg gggtcgcgct
    13381 caccggcgga ggctacagcc ctccgcgacg ccccggccgg gaccgtgtct cgtcaccgct
    13441 acgtcacagt ctcgtccccg tgcctggcac cgggacgtgt ccctttgtcg ctcagtgccc
    13501 ctaccagcaa tgcacgacgg cttctgcccc gcgatcacgc agcgccagag tccaccctcg
    13561 cccttagcaa ggcagaccag ccttgctgcg gtcgagtgcg gcctctggct tggtgctcgc
    13621 gacgcagaaa ctcgccagca tcactggcag gggcactagc cgtgaatcga gacgatcagc
    13681 gggatgatca ggatggcgac gatgttggcg atcttgatca tcgggttgat cgcgggtccc
    13741 gccgtgtcct tgtacggatc gccgaccgtg tcgccggtga ccgacgcagc atgagcctcg
    13801 gatcccttgc caccgaaggc tccgtcctcg atcagcttct tcgcgttgtc ccacgcgccg
    13861 ccgccggacg tcatcgcgat cgcgaggaac agccccgtca cgatcgttcc gatcaacagc
    13921 ccgccgagca tgcgcgcgtt gatgatcccg acgatgatcg ggaagaggat cgggatcaac
    13981 gcgggcaaga gcatcgcctt gatcgccgag ctggtgacga tgtcgatcgc acgcccgtat
    14041 tccggtcgac tcgtcccctc catgattccg gggttctcac ggaactgacg tcgcacctcg
    14101 gtgacgacct ctccgccgac gcgcccgact gcctgcatgg ccagcgacgc gaacaggaac
    14161 ggcatcagtc cgccgatgaa gagaccggcg atcacccagg cgtcgctgag gctgaacgtc
    14221 gacgtcaggc cttcgtccgc gagtccggtc gtatacgagt cgaagagcac gagcgccgcg
    14281 agcgcagccg agccgatcgc gtagcccttc gtcaccgcct tcgtggtgtt gccgacagcg
    14341 tcgagcggat cggtgatcgc ccggacggat tcgtccaggt tcgccatctc ggcgatgccg
    14401 cccgcgttgt ccgtcaccgg tccgtacgcg tcgagggcga cgatcaggcc cgtcatcgag
    14461 agctgggcca tcaccgcgat cccgatcccg tacagcccgg cgaagtgatg ggcgatgagg
    14521 attccggccg cgatcacgac caccggcagt gccgtggctt tcatcccgac ggcgaggccc
    14581 tcgatgatgt tcgtcgcgtg gccggtctgg gacgccgacg agatggactt caccggtccc
    14641 cagcgtgtgc ccgtgtagta ctccgtgatc gcgacaagca agaacgtcac gacgaggccg
    14701 acgagcgacg cgcagtagag gtcggtgaag ctgaacttgc cgccgtcgta ggccatcgtc
    14761 accgggatga agccgatcgc cgacagcacg gtcgcgacca gcacggcctt gtagagcgcg
    14821 ttcatgatgt tgcccgtgcg cccgacgcgt gcgaagaacg tgccgatcac agacgcgagg
    14881 atcgagatgc cgccgagcgc gagtggatac agagcgagcc cgctcgacgg gaactgcgtg
    14941 cccagcagca tcacggcgac cgcagtcacg gcatacgtct cgaagagatc cgcagccatg
    15001 ccggcgcagt cgccgacgtt gtcgccgacg ttgtccgcga tcacggccgg gttgcgcgga
    15061 tcgtcctccg ggatgcctgc ctcgatcttc cccaccaggt cggccccgac atcggcggcc
    15121 ttcgtgtaga tgccgccgcc gagccgcgcg aagaccgaga tgagggagcc gccgaaggcg
    15181 aggccgatca gatcgtggat cgccgagtcc gccgagttgc ccaaccagcc ggtcagagcc
    15241 cagtagtacc cggcaacgcc gagcagggcg agcccgacga ccatcattcc ggtcaccgac
    15301 cccgcgcgga acgcgacgtt gagagcaggc ttgagcccgc tccgcgccgc ctcggccgtc
    15361 cgcacgttcg cgcgcaccgc gacgttcatg ccgatgaacc cggcgcctgc ggagaggatg
    15421 gcgccgacca ggaaaccgat cgccgtgccc cagccgagct tgttgtagaa gccgagtagg
    15481 aggaacggca cgatcgagac ggcggcgatc gtcgtgtact gccgccgcaa gtaggcggca
    15541 gccccttcct ggaccgcctt ggcgatctcc cgcatgcgtt cgttgccgtc cgggcggccc
    15601 agcagccaga aggtcagcca gatgccgtag gcgatggcga cgcccgcaca gaccagcgca
    15661 aacagcaccg gatggtcgtt gaagaagctc ccgacgatca gcgttcgtac cccctcacaa
    15721 agtccgcgcc gagtgacctc ggcgcggcgg gttttctatc acatggcgcc ccctggagcg
    15781 ccgtttcagc ctcagacggc cgcttcgtca gtgaccgccg ggcgggcgag ctcgagtggc
    15841 gacagtgtcc cgacgagctc gggatgggcc ttgcgaacgt gcaggtcggc gtcctggagg
    15901 agctgctcct cgtcctcccc ccagaggacc tgaccacagc cgcagtggat tactccggcg
    15961 acatcgatag ccatcgcttc aacaggacgg cgtggtcgtg cctgctcttc ctcgctctca
    16021 accctcgccc ttggcggcgt ccgcgtacgc gagctggagg ttcgcagtca cctgcttgaa
    16081 gtcccgcacg agctctgcgc ctgctgctcc ctcgagcacg ggcaggagtg cgcgcagggc
    16141 ttcgatgccg agccgcgcct ggtcgaggtc gcggccctcc tcggagagcc ggcggtatcc
    16201 gagcgacgag acggtgtaga gcgtctggac gaggacgtcc gacacctcga gcgtcttgag
    16261 ctcctcctcg agttgcttca ggagctcgtc gtcggagggc ggcccgctct cggtcacgat
    16321 ggctcccaca gctcgaagcg gtttccctca acatcgctcg cccaggcgaa tcgtccgtac
    16381 tccatctcct cgacccgatc ctcgacgtcg acaccggccg cgcggagctg cgcgagcatg
    16441 gcgtcgagat cgcggacgcg gaagttgacc atcagctgct gtccacccgg gccgaaatac
    16501 ctggtgtccg ccggaaaggg tgcccagacc gtctggtcgc cgtccttcga ggtgaatgtg
    16561 gtcccgtaat cctccatggc gatgccgagg tgttcggcgt accaggcgcg cagagcgacc
    16621 ggatcgctcg cgcggaagaa gacgccgcct actccctcga ccgcctcacc caccgacccg
    16681 ctcccgtggc ctgcggacca gctccgcgaa aacgtcgcga tgcgagccga gctccgcaac
    16741 gcgctcgtac tgacgctcgg cggcgttcgc cgcgggaact gcgaggtaag gagctgcgcc
    16801 gatctcctcg gcgaccggaa ggacccactc gatcagggcc tcgaccctcg ccctcgccgg
    16861 aacgacctcg ccgcggtcga agtcgatcat cccgtccgtc agcccgtacc ggatcgcgcg
    16921 ccacatgttc tcctcgatga gtcggtgcgg taggtcgggg agcgcttctc cttcgtcgag
    16981 cgcgcgtgcg aagcgcgtcg ccagcgcgta gatcagcgcc gccagcgact gcgcctgcgc
    17041 cagatcgggt tgcgcgtcgc agatccgaac ctcgaccgtc ggaaacgcca gatgcggtcg
    17101 cacgctccac cagatctgcg tgtgctcgtc gatcgacctg gtcgcgaaga gaaagcggac
    17161 gtactcctcg tacgcggtcc aatcgtcgaa cgcgtccgga acgccgcagc gcggaaacag
    17221 cttcgtgaag atctcggttc gcgccgagtg gaggcccgtg acgcaatcct cgaggaacgg
    17281 tgagctcgcg gacaccgcca ggagctccgg caggacgttg cggaaggcat tgcacacggc
    17341 gaccgcgcga tcggcgccgc gaatgccgat gtgggcgtgg atgccgaagc tgttgttgcg
    17401 ccagacgacg tactgcagca gctcgttgtt ccgccggtag tgcggcgtgt cgatgattcg
    17461 ctggtcctgc cagcggctcc acggatgggt cccggtcgaa gctagcccga ctccgaatcg
    17521 gtcggcgagc tcccccagct gcgcgcgacg ctcggccatg cgctccgcgg cttcggagaa
    17581 gttctcgcaa cggccggtct tgacctcgat ctcggatgcg atcagctcgc cgacgaggtg
    17641 ctcgtcgagc tccgtccccg ccgccccggc cttgagctcc tcgaagcgac tcgtcaattc
    17701 gagcgtatcc ggatcgagca gcgcgaattc ttcctccact gcgatcgtga agtcgtcgcc
    17761 gttctcgaaa gcctcgcgcg ccaaaccgat ctgcgcctcg ggagcgagtc gttgctggtc
    17821 acccatgttc acaagggtaa atgaagcaat agcggctaac cgccgcggat cggctccagc
    17881 agccgtgtgg cgacactcgt ccagctccag cgctcgacgg cagctcgccg cgcggcggcg
    17941 ccgagctctg cgcgtgctgc cggcggcagg gcgagcagct cgtcgagctt cgctgcgagg
    18001 tcggctgcgt caccgttcgc gaagctcgcc aggtgcctgt actcagctgg atactcggct
    18061 tcgaggcctt ccgcgatctc cgcgagcccc gagtggcgag ccacgagcgg cggggagccg
    18121 gccgcagccg cctccgcggc gaccatgccg aaggcttccg ggaagatcga cgggacgacg
    18181 gtgacgtcgg cgagcgggag caggtgcacg aggtgtcggt gttcgagtgg gccggtgaac
    18241 agcgtccccg gcggagcaat cgactccagc tcctgccggt agtcgccgaa cccgacgatc
    18301 agcgcgcgtg cgtcgaggct gttcagcgcc tcgaacagga ggtgaacgcc tttgttgtac
    18361 aggagcttcc cgaaatagac gaccgtcggt tcgtctgtgt cgaagaattg ctcgaggcgc
    18421 gtggcgttgc cctcgtccgg ttggcgctca tctgagttcc ccgggttgtc cgcgtccgct
    18481 cgcgcttcct ccagcaaccg cgagagggat tcctcgcgtg agcgggaggg ccgaaactcc
    18541 tcgacgtcga cgcccggcgg cacctcgtgc acgcggtcga cgtggccgac gacctcctcc
    18601 aggacagtcc ggatgtgcgc cgacccgaca aagaccgcct ccgcaagtgc gaggcactcg
    18661 cgaccccagg cagcgagctc ctcgttgccg cgcatcgagt actcgagctc tgagccgtgc
    18721 gccttgacgg caaacggcag tccgctcgcg taaccgaccg cgccgccgag cagcacgtgg
    18781 ttcgcgaaga ccaggtcggc cggcatctgc gcgcacaacg cggtcgcgtt gagcgcgacg
    18841 tagcgtgccc tttcgtcgat ggtgaaatcc tgcaacagct tcgcctccac accctcgtac
    18901 cgatcgagca cgaaaacggg aagcaacccg ccgatgttcg gacggaacac ctccgctcct
    18961 ccgaggtcgt aacgctcggg atgaggctcc tgacagatca cgagaacgtc gtggccggcg
    19021 cggctccatt cacgcgccag ggaacgcgtg tagacgttgg accctgttcc accgagcagg
    19081 tagccgtgcc agagcaggat ccgcgtggtc ccgcctcctg gggctatgt
//
LOCUS       DASVEU010000167         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1088359,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000167 DASVEU010000000
VERSION     DASVEU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088359"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VF225_06700"
     CDS             <1..585
                     /locus_tag="VF225_06700"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX7144983.1"
                     /translation="GLTVDDEGELVLLCVPDGSIAEVASGLEEGPWVAHVSGATPLSA
                     LDPHRRRFSMHPLQTFTRARGPEQLDGAWAAVTAEAEEARVAGFWLAETLGLKPFELA
                     DAARPLYHAGAAIASNYLVTLHAVASELFRAAGAPPEALVPLMRRTIENGFELTGPIE
                     RGDWETVAAHRRAIRAARPELEPLYDVLAEATAK"
     gene            582..1370
                     /gene="panC"
                     /locus_tag="VF225_06705"
     CDS             582..1370
                     /gene="panC"
                     /locus_tag="VF225_06705"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7144984.1"
                     /translation="MKTLRKLREVRQALAGDGRVGLVPTMGAFHEGHLALFRAARDEC
                     DVVVISLFVNPTQFGASEDLSRYPKDEARDAELAEAAGVDILFAPSAEEIYPEGYATW
                     IDVGDTGAEGNARPDHFRGVATVCLKLFNIVQPQVTYFGQKDAQQANVLRRMIRDLNV
                     DLELRVLPTVRDSDGLALSSRNAYLSEEERRQALVLPRALAAGRSAHEQGSDPVTAAR
                     GAFNGLEPDYVEVLNLGDVTVLTAAARVGSTRLIDNIVLEGELT"
     gene            1367..2329
                     /gene="panB"
                     /locus_tag="VF225_06710"
     CDS             1367..2329
                     /gene="panB"
                     /locus_tag="VF225_06710"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7144985.1"
                     /translation="MKPGKLTLTQLADMKHRGEKVVMVTAYDAPGARLAEQAGIDILL
                     VGDTAGMVVLGHDGTVPVTMDEMLFLTRSVSGAVERPLVVGDMPFGSYHVSDEDALRN
                     AIRFVKEAAADVVKLEGAGPSLSRVRAIVSAGIPVMGHVGLTPQSETILGGFKTQGRT
                     AAKARQLLADALALEAAGCFAIVLEAVPVPVAARITESLTAPTIGIGAGAACDGQVLV
                     YHDLLGLTEGRAPRFVKRYANLAEEIRGALTTYAEEVRTGIYPAPEHTYAMPEDELEL
                     FSDAFSSERPEGGTASGASAERVEQGQRNGQSHGDQRRERDARH"
     gene            complement(2249..2905)
                     /locus_tag="VF225_06715"
     CDS             complement(2249..2905)
                     /locus_tag="VF225_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX7144986.1"
                     /translation="MVTRVESGGDEREWFLRALLVLQSPRRVFAALRDDSDEVAAARQ
                     DTAGAIVWLAGIAAVLATTVASTLLDDVQIDGSLLAVWAFFAGGLYGFTVYFVVGKVL
                     HVALRRLGGRGSFRRARHVLAFAAVPIAAALFVYWPIRIAVYGWDLFRTGGADSHGGG
                     PLVAWIFYGFVIWSLGLLLVGVRTVHGWTWARSVAGVAFASLIAVGLAVALTLLYALG
                     "
     gene            2934..4115
                     /locus_tag="VF225_06720"
     CDS             2934..4115
                     /locus_tag="VF225_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144987.1"
                     /translation="MTPVEVEMPRTVVLLIVLFLAPPAHAATQCDAPRPRGPVGLADT
                     ILVKGACGTFALRRDGSVELVHPRPWAPAWAPGALARADDRTYIAHPRHHLVLLRDAE
                     VLWRSRLPHGSDNVVVHGQAIAFTAYRRPTPDLWVARFGTPERLVGPGEDLQGWARAD
                     GFFTEHAHELRLRAADGRLVRRIARVKWSTYDRATQSVFAISNSNRLIRTDGRQTTTL
                     ADLRSIGLAQHPWLEVLPRGLIHIGSGNRMLLVRHDGTRFASASLARPRGGNLGETIV
                     SALLPLPADRGVVFVVNSRRTEKGSSMDRVLLLERGHSKTRLLYERRAIPLQCAYWAN
                     LSLHGNSVLYWPSGGRALVALNALGQRAPIDLWPVLLRTPGFRHQGWLRRAAWASTWN
                     S"
     gene            complement(4125..>4692)
                     /locus_tag="VF225_06725"
     CDS             complement(4125..>4692)
                     /locus_tag="VF225_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144988.1"
                     /translation="YVPCETCKGARYNRETLEVRFKGKSIADVLEMSVEEALQFFAKI
                     PKLRRRLQTLHDVGLDYIKLGQPATTLSGGEAQRVKLSSELSKIATGKTLYILDEPTT
                     GLHFADIEKLLDVLQRLVDSGNTVLVIEHNLDVIKQADWIIDLGPEGGEAGGEIIATG
                     TPEQIAEVDESATGQFLRHVLPKTAVAA"
BASE COUNT          761 a         1635 c         1559 g          737 t
ORIGIN      
        1 gggctcaccg tggacgacga aggcgaactc gtcctcctgt gcgtgcctga cggttcgatc
       61 gccgaagtcg caagcggcct cgaggaaggg ccctgggtcg cccacgtctc cggagccacg
      121 cctctgtcgg cactcgatcc gcatcgccgc cgtttctcga tgcacccgct gcagacgttc
      181 acgcgcgcgc gggggcccga gcagctcgac ggagcctggg ccgcagtgac ggccgaagcc
      241 gaggaggcca gggttgccgg tttctggttg gcggagaccc ttggcctcaa gccgttcgag
      301 ctcgccgacg ccgcgcgtcc gctctaccac gcgggcgccg cgatcgcgtc gaactatctc
      361 gtgacactgc acgcggtggc gtccgagctc tttcgcgcgg caggcgcgcc gccggaggca
      421 ctcgttccgc tgatgcgcag gacgatcgag aacggcttcg agctgaccgg gcccatcgag
      481 cggggcgact gggagacggt ggcggcgcac cggcgcgcca tccgcgcggc aaggcccgag
      541 ctcgagcccc tgtacgacgt gctggccgaa gcgacggcga agtgaagacg ttgcggaagc
      601 tccgtgaggt gcgtcaggcg cttgcgggcg atgggcgcgt cgggctcgtg ccaaccatgg
      661 gcgctttcca cgaaggacat ctcgcactgt tccgcgcagc gcgtgacgag tgcgacgtcg
      721 tcgtcatatc gctgttcgtc aacccgacgc agttcggcgc gagcgaggat ctgtcgcgtt
      781 acccaaagga cgaggcgcgt gacgcggaac tcgcagaggc ggcaggagtc gacatcctgt
      841 tcgcgccttc cgccgaggag atctatccgg agggatatgc aacttggatc gacgtcgggg
      901 acaccggagc cgaaggcaat gcgcggccgg accacttccg aggtgtcgcc acggtgtgcc
      961 tgaagctctt caacatcgtg cagccgcagg tcacctactt cggccagaag gacgcccagc
     1021 aggccaatgt cttgcgtcgg atgatccgcg acctcaacgt ggatctcgag ctgcgcgtcc
     1081 tgccgaccgt ccgcgactcc gacggactgg cgctttcctc ccgaaacgcc tatctgtcgg
     1141 aggaggagcg gcggcaggcg cttgtgcttc cacgcgctct cgcagccggc cgttccgcgc
     1201 atgagcaagg gtctgacccc gtcaccgcgg cacgtggcgc gttcaacggg ctcgagcccg
     1261 attacgtgga ggtactcaac ctcggtgacg tgaccgtgct cacagccgcc gctcgcgtcg
     1321 gctcgacgcg actcatcgac aacatcgtcc tggaaggaga actgacgtga aaccggggaa
     1381 gctgacgctg acgcagctcg ccgacatgaa gcatcgcggc gagaaggtcg tcatggtcac
     1441 cgcgtacgac gcgccgggcg cgcgactggc cgaacaggcg gggatagaca tcctgctcgt
     1501 cggggacaca gccggaatgg tcgtgctcgg tcacgacggc accgtgccgg tgacgatgga
     1561 cgagatgctt ttcctgacgc gctccgtgtc cggcgcggtc gagcgaccgc tggtcgtcgg
     1621 cgacatgcct ttcgggagct accacgtctc cgacgaggac gccctgcgca acgcgatcag
     1681 gttcgtcaag gaggccgccg ccgacgtggt caagctcgag ggcgccgggc cctcgctgtc
     1741 ccgtgtgcgc gcgatcgttt ccgccggcat ccccgtgatg ggacacgtcg gcctgacccc
     1801 gcagtcggag acgattctcg gcggcttcaa gacacagggt cggacggccg cgaaggctcg
     1861 ccaactgctg gccgacgcac tggcgctgga ggcggccggc tgcttcgcga tcgtgctcga
     1921 ggccgtgccc gtccccgtcg ccgcgcgcat caccgagtcg ctcacggctc cgacgatcgg
     1981 gatcggcgcg ggagctgcgt gcgacgggca ggtgctcgtc tatcacgacc tgctcgggct
     2041 caccgaggga cgcgcaccgc ggttcgtcaa gcgttacgca aacctcgccg aggagatccg
     2101 cggagcgctc acgacctacg ccgaggaagt ccgcaccggc atctatcccg cccccgagca
     2161 cacatacgcg atgccggaag atgagctgga gcttttttca gacgcgtttt cgtccgagcg
     2221 tccagaggga ggcaccgcaa gcggcgcctc agccgagcgc gtagagcagg gtcagcgcaa
     2281 cggccagtcc cacggcgatc agcgacgcga acgcgacgcc cgccactgaa cgggcccagg
     2341 tccagccgtg cacggtccgg accccgacga gcagcagccc tagcgaccag atgacgaagc
     2401 cgtagaaaat ccaggcgacc agcggcccgc cgccgtggct gtcggcgcct cccgtacgga
     2461 agagatccca gccgtagacg gcgatccgga tcggccagta aacgaagagc gccgccgcga
     2521 tcgggacggc tgcaaaggcc agcacgtgtc gtgcccgccg gaagctgccc cggccgccca
     2581 gtcgccggag cgccacgtga aggaccttcc cgacgacgaa gtagaccgtg aagccgtaga
     2641 gaccgccggc gaaaaacgcc cagactgcga gcagcgagcc gtcgatctgg acgtcgtcga
     2701 ggagcgtgct cgcaaccgtc gtggcgagga ccgccgcgat cccggcgagc cagacgatcg
     2761 cgccggccgt gtcctgccgt gccgcagcga cctcgtccga atcgtcacgc agagcggcga
     2821 acactcttcg cgggctctgg aggacgagga gtgcacgtaa gaaccactcc ctctcgtcgc
     2881 cgcccgactc caccctcgtg accacccgag cagcgtagtg gaccccgtgc cgcatgaccc
     2941 ccgtagaggt ggagatgccc cgcaccgtcg tccttctcat cgtgctcttc ctggcgccgc
     3001 ccgcgcacgc ggcgacacag tgcgacgccc cgcgtccacg cggcccggtc ggcctggccg
     3061 acacgatcct cgtcaaaggt gcgtgcggca cgttcgctct ccgccgggat gggagcgtgg
     3121 aactcgtgca tccgcgcccc tgggctccag cctgggcacc gggcgcgctg gctcgtgccg
     3181 atgaccgcac ctacatcgcc catccgcgac atcacctcgt cctcctgcgc gacgcggaag
     3241 tgctgtggcg ctcacgcctg ccgcacggaa gcgacaacgt ggtcgtgcat ggccaagcga
     3301 tcgcgttcac cgcctacagg cgtcccaccc ccgacctctg ggttgcccgg ttcgggaccc
     3361 cagaacgtct tgtcgggcca ggagaggatc tgcaggggtg ggcgcgtgcc gacggtttct
     3421 tcacggagca cgcacacgag cttcggctcc gcgcggccga cggccgactg gtcagacgga
     3481 tcgcccgcgt caagtggagc acttatgacc gagcgactca gtcagtgttc gcgatcagca
     3541 actcgaaccg gctgatccga accgacggtc ggcagaccac aacgctcgcg gacctgcgct
     3601 cgatcggcct tgcccaacac ccatggctgg aagtgctgcc gcgcggcctg atccacatcg
     3661 gctccggtaa ccgcatgctt ctcgttcgcc acgacgggac gcgctttgcc tcggcctcgc
     3721 ttgcccggcc gagaggcggc aacctcggcg agacgatcgt cagcgctttg ctgccgcttc
     3781 ccgccgaccg cggggtggtc ttcgtcgtca actctcggcg cacagagaag ggcagctcta
     3841 tggatcgtgt gctcttactc gagcgtggcc acagcaagac gcgccttctg tacgagcggc
     3901 gagcgatccc gctccagtgt gcctactggg cgaacctctc cctgcacggc aacagcgtgt
     3961 tgtactggcc cagcgggggt cgggcgctcg tcgcgctcaa cgcgctcggg caacgcgcgc
     4021 cgatcgatct gtggccggtg ctgctccgca cgcccggctt ccggcaccag gggtggttgc
     4081 gtcgagcggc ctgggcctca acctggaaca gctgacgctc ccggctacgc ggcgacggct
     4141 gtcttcggca agacgtgccg aaggaactgc ccggtcgccg actcgtcgac ctcggcgatc
     4201 tgctccggcg tgcccgtcgc gatgatctcg ccgccggcct cgccgccttc gggaccgagg
     4261 tcgatgatcc agtcggcctg cttgatcacg tcgaggttgt gctcgatcac gagcacggtg
     4321 ttcccggaat cgaccaaccg ctgcagcacg tcgagcagct tctcgatgtc cgcgaagtgc
     4381 agcccggtcg tcggctcgtc gaggatgtag agcgtcttcc cggtcgcgat cttcgagagc
     4441 tcggacgaga gcttgacccg ctgtgcctca ccgcccgaga gcgtcgtcgc cggctgtccc
     4501 agcttgatgt agtcgagacc gacgtcgtgc agcgtctgca gccgccgccg gagcttcggg
     4561 atcttggcga agaactgcag cgcttcctcc actgacatct cgagcacgtc tgcgatcgac
     4621 ttccctttga agcgcacttc cagcgtctcg cggttgtagc gcgctccctt gcaggtctcg
     4681 cacgggacgt ac
//
LOCUS       DASVEU010000168        14548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_313432,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000168 DASVEU010000000
VERSION     DASVEU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313432"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF225_06730"
     CDS             <1..210
                     /locus_tag="VF225_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144989.1"
                     /translation="AVYLASDEAAFVHGTVIDVDGGRTGVAVIAPGPDTRSHTAPFTS
                     RDGGRAKSSAPSDRDKFSRTRRIQE"
     gene            223..693
                     /locus_tag="VF225_06735"
     CDS             223..693
                     /locus_tag="VF225_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7144990.1"
                     /translation="MAATLISLTASVNGVLYELGVAGRDTLLDVLRERLALTGTKKGC
                     DQGACGACTVLVDGRRVLSCLMLAAQCDGCEVTTIEGLGSEAQLHPLQSAFVRHDALQ
                     CGYCTPGQIMSAVALLDEGRAGSDDEIREFMSGNLCRCGAYPNIVAAIRDVAGR"
     gene            690..1673
                     /locus_tag="VF225_06740"
     CDS             690..1673
                     /locus_tag="VF225_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7144991.1"
                     /translation="MSGTFEYVRADNVAAAVALVSADPDAQYLAGGTTQVDLLKDGVL
                     NPERLVDITRLPLRGITRRGDALVVGSLTTMEELAAEPVLGARATFVRAALLAGASTQ
                     LRNMATIGGNLMQRTRCRYFRDPTVPECNKRASGSGCAAISGPARMHAILGASKHCIA
                     PHASDLCVALVALDTLVHVQGTDGRRTIPLTQFYRVPGDRPDVENVLAHGELITAIEI
                     PLLPAGARSEYLKVRDRASYEFALTSAAVALTIEDGVIAEARVALGGIGTFPWRAHAA
                     EEVLSGAGASTSLFRAAADAEIVDPFTLPGTAFKVELAKRTLVRALETAAA"
     gene            1670..3865
                     /locus_tag="VF225_06745"
     CDS             1670..3865
                     /locus_tag="VF225_06745"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7144992.1"
                     /translation="MTALIGSGVDRVDGPAKVRGAAPYPTDVTYPQLAHAALVRSTIA
                     SGRVIAINDHDARATAGVLTVITHETAPTLTPAPFGLLGPAPPPPLQDDHIHHHGQYV
                     AVVVAATAHEADVAARLVKVEYEGGDPPLLDFDDPRSALEATPWDTATQRGDVEAGLA
                     EADVIHEATYTTADNTNNPLGLFATVAAWEGDIVTVHDATQWPAYTQATIAEVFGIEP
                     AAVHVRSPYVGGAFGAGLFVWQHVILAVLAARAVGRPVKLVLTRPQMFTGVGHRPSSV
                     QSIRMGARRDGELVAIEHVSTNSASIKHASIEPISGATARSYACANVATTDQQRRLNI
                     PLPGSMRGPGHAQGNFALESAIDELAYALGLDPLELRLRNFAAVHPQSGLPWSSNALG
                     ECYTVGAERFGWSRRDPEPGSMREGHWKIGYGLAAISWSWWQVRCQARATITRDGRAH
                     VCSAASDPGTGTRTVMRQVAGERLGLPLNDVRFELGDSEMPWAPASGGSSLTASLGNA
                     VHAACEALVRRFLDVVADDDRSPLRGCSLDDVRVAEGRIHRRDDPSDGEHYTDILARH
                     GLNELTVDAEATPPSQENAELARSGPFAAKFVEVRVDEDLGLIRVARITTAIDAGRIL
                     NEKTARSQIVGGTIGGIGQALLEETITDPGTGRITNATFGDYLIPVNADTPDIDVFFV
                     GRPDPLTPLGAKGLGEIGLVGIAAAIANGVHHATGRRIRSIPITLDRLL"
     gene            complement(3991..4063)
                     /locus_tag="VF225_06750"
     tRNA            complement(3991..4063)
                     /locus_tag="VF225_06750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4028..4030),aa:Thr,seq:ggt)
     gene            complement(4255..5343)
                     /locus_tag="VF225_06755"
     CDS             complement(4255..5343)
                     /locus_tag="VF225_06755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7144993.1"
                     /translation="MRRIDALTFFSAEEVARARSYHRPLYWAGIADVVIEASVLATLV
                     WSGAGAALDPGSLPWWGRTPAYAAIVVAATTAVRTPLPLWSGLIRERRWGFSTQRLPS
                     WVADRAKAVGLNIVLTAVALLGLVGFARALPGWWVAPAAAAFAFATLMLSFLAPVVLE
                     PLFNRYSPLQDATLAAELGRLAERGGVPIRDVLVQDTSRRTRKANAYVSGLARTRRVV
                     VSDTLVEQASPAEVRLVVAHELAHRRERHVLLGTLLAMTGAATATVVVWALLGTRVGD
                     PHQVPLVLLIGLGIALTSLPALTAISRRWERAADRYSLELTGDRAAYATVFRRLAQTN
                     LTDLDPPRLLYLLLFTHPTPPERLAAAD"
     gene            complement(5406..5582)
                     /locus_tag="VF225_06760"
     CDS             complement(5406..5582)
                     /locus_tag="VF225_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144994.1"
                     /translation="MLSGLRAKLWRRKLDQQEARSEPAVDERSSGEEAIETADEEAAE
                     EAPSGMTRLKTDDL"
     gene            complement(5852..6751)
                     /locus_tag="VF225_06765"
     CDS             complement(5852..6751)
                     /locus_tag="VF225_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7144995.1"
                     /translation="MSIDLMNRQFDRTDDRTSSEVDSSYELEDSLGLYARGVRRVPML
                     SPAEERELFRRKEAGDERAKRILIEANLRLVIWIARKYAHHNVPLLDLIQEGNLALTR
                     AVERFDHRLGFRLSTYATLMIKGAVGRAAECHGRLVPVPIHVGRQLRTVRRARQVLLE
                     RLNRDPLLCEIAAETGLDSRRVGELLGYEQQPVSFESPPKEGQQACSELLADPTSAHL
                     DAALAERQRKEEVEATVNSLDERLRLVLRLRFGLAGQSPRSLVEVGRELGVTSERARQ
                     LEATALDKLRMLAPGLRDYLEVA"
     gene            complement(8297..9358)
                     /locus_tag="VF225_06770"
     CDS             complement(8297..9358)
                     /locus_tag="VF225_06770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7144996.1"
                     /translation="MTPLRDLLETSVERGTVPGAAALVARGDDVEVAEAGEVERESIV
                     RIASITKPITAAAIMILVDDGLVSLDDPIARWLPELASPLVVRTPESAIDDVVPATHP
                     IAVEDVLTFRAGWGFPSDFSLPAVVELFRKLPVFGPRDTPDEWLATLAQVPMLRQPGE
                     AWLYNTCSDVQGVLIARVSRQPLPEFLAERIFEPLGMSDTSFHVPSEKLDRLPPYRGP
                     DFAPIDDGLWTEPPIFPSGAGGLVSTLSDWHRFGRMLLADGGALLSPESVRLITTDHL
                     TQEQRDASTLFLEGAGWGFGGAVAADGRYGWVGGTGTSAHVASSSGTIGILLTQLQMT
                     GPTPTQFMREFWRYAFGAS"
     gene            9457..9858
                     /locus_tag="VF225_06775"
     CDS             9457..9858
                     /locus_tag="VF225_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7144997.1"
                     /translation="MSKVASKLNLTQVGRVCVTVGDTDRAIEFYVDTLGFEKVVDVPM
                     GPEMRWVEVALAGTPTTIALAPPPQDEEAGGSQTGICLDTTDVDADHAALKAAGVDVD
                     QQVTRWGGPVPPMFWLRDPDGNSLIIVQPSD"
     gene            11092..12369
                     /locus_tag="VF225_06780"
     CDS             11092..12369
                     /locus_tag="VF225_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX7144998.1"
                     /translation="MQTVAVVGAGPKGIALAAKARALADVGFDAPQVVLIDRAGIAES
                     WTGRRGYTNGLLPLGTPPEKDVGFPYAESWGGASAEVRDAMTRFSWQHHLIERGEYAD
                     WVDRGHLRPSHRRWSTYLRAVAQKAEADVIEADVVGLELEDERWRLSLADGAALVADG
                     VVLTGAGPAIEIAGQPRGHPRVLDGRSYWLHEKEFKKHFGLNVCVIGSGETAASIVIS
                     LLKKLYKGSTVDVLTSRGVLYSRGESFDENRFYSDPGDWPRLAETHRREFLERTDRGV
                     FSLQAEATLNQSRCFRTLAGRAVAIDPGGRQVLVTIEYGDEQERVVYDLVVVAIGFEA
                     RWFESFLGREASARLVDALADDELERRIDIDLGVDGLRPRLHLPMLAGLAQGPGFPNL
                     SCLGLLSDRVLRSYVDGLTPRTPDQLGERRQLV"
     gene            12362..12541
                     /locus_tag="VF225_06785"
     CDS             12362..12541
                     /locus_tag="VF225_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7144999.1"
                     /translation="MSDTQDEVWESDELVRRTIAIPRRLAERLAAEAERRDLGINDLI
                     VELAEEGLPEPAADD"
     gene            complement(12615..14096)
                     /locus_tag="VF225_06790"
     CDS             complement(12615..14096)
                     /locus_tag="VF225_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145000.1"
                     /translation="MTGNGNTRRRPSNIAVAAAGRLARAGSNALTTTLGGRERTRVIL
                     LLASVLALASADSATVGASAIQLRHDLHINNTDVGLLVTVTSLVAAAASLPFGVLADR
                     VRRTRLLGGAIVLWGAAMIWSATAPTFGQLLLARLFLGGVTAAAGPVVASLVGDWFAS
                     SERGRIYGYIISGELVGAAFGFAVTGGIATVSWRASFVILALPAFALAWLVFHLPEPT
                     RGGGKPLLSATATPVPAAAGDDGEDQATDAQRLAGERGVEPDLQLVLTRDPRRLGLLA
                     AARYVLSIRTNVLLILASAGGYFFLSGVQTFGVEFVTKQYGIAQALSALVLLAVGAAG
                     VIGVLLGGWLGDFLLHRGFLNGRILVSAIAATVAVILFVPAIFIRSPLTALPYISLAA
                     LALSAQNPPIDAARLDIVHPLLWGRAEGIRTALRTLAQATAPLLFGALSDYLFGGGRS
                     GLQWTFAFMLLPLAGSAYFLFRALRTYPRDVATAAASTAASRS"
     gene            complement(14200..>14548)
                     /locus_tag="VF225_06795"
     CDS             complement(14200..>14548)
                     /locus_tag="VF225_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145001.1"
                     /translation="SALLAFGGPSWLASFQASLAGWVSHHGLATVVVLVAVEALIGLT
                     ALSRRTVAVSAAAGLLALLAIWVVGQDFGQLYTGQATDPNTALIATLMAVALLAAYYH
                     RPREYEDRLRRRT"
BASE COUNT         2481 a         4881 c         4940 g         2246 t
ORIGIN      
        1 gccgtttatc tcgccagtga cgaggctgcg ttcgtccacg gcaccgtgat cgacgtcgat
       61 ggcgggcgta caggcgtggc tgtcatcgcg cccgggccgg acacccgatc acacacggcg
      121 cccttcacct cacgtgacgg cggccgggcg aaatcttccg caccttccga tcgcgacaag
      181 ttctcgcgca caagacgaat tcaagagtga aaggagcctt agatggccgc aaccctgata
      241 tcgctgacgg catcagttaa tggtgtgctg tatgagttgg gtgtcgccgg ccgagacacg
      301 ctgctggatg tcctccgtga gcgactggca cttaccggca ccaagaaggg ctgcgaccag
      361 ggcgcgtgcg gagcctgcac ggtgttggtc gatgggaggc gtgtgctttc gtgcctgatg
      421 ctggccgccc agtgcgatgg gtgcgaggtc acgacgatcg aagggctcgg cagcgaagcc
      481 cagcttcatc cgttgcagtc ggcgttcgtg cgccatgacg cactgcaatg cgggtactgc
      541 accccgggtc agatcatgtc ggccgtcgcg ctactcgatg agggccgagc tgggtccgac
      601 gacgagatcc gcgagttcat gagcgggaac ctctgccgct gcggcgccta cccgaacatc
      661 gtcgccgcta tccgtgacgt cgcgggacga tgagcggcac gttcgaatac gtgcgtgccg
      721 acaacgtcgc cgcggcggtc gctctcgtga gcgccgatcc cgacgcgcag tacctcgccg
      781 gaggaacgac acaggtcgac ctgctcaaag acggcgtgct gaaccccgag cggctggtcg
      841 acatcacacg cctgccgctc cgcgggatca cgcgacgcgg cgacgcactc gtcgtcgggt
      901 cgctaacaac gatggaggag ctcgcggccg agccagtgct cggcgcgcgg gcgacattcg
      961 tgcgcgcggc actgctggcg ggcgcctcga cccagttgcg caacatggcg acgatcggcg
     1021 gcaacctcat gcaacgcacg cgctgccgct acttccgcga tccgacggtg cccgaatgca
     1081 acaagcgagc gtccggttcc gggtgtgcag ccatcagcgg tcccgcgcgg atgcacgcga
     1141 tcctcggcgc aagcaaacac tgcatcgcgc cgcacgcatc ggacctctgc gtagcactgg
     1201 tcgcgctcga caccctcgtg cacgtccagg gcacggacgg ccggcgcacc atcccgctca
     1261 cccagttcta ccgcgtgccg ggcgatcgac ccgacgtaga gaacgtcctc gctcacggcg
     1321 agctgatcac tgcgatcgag atcccgcttc tccccgccgg cgcacgctcc gagtacctga
     1381 aagtacgaga ccgcgcctcc tatgagttcg cgctcacctc ggcggccgtg gcgctcacga
     1441 tcgaggacgg cgtgatcgcc gaggcgcgcg tcgcactcgg cggcatcggg acgttcccgt
     1501 ggcgcgcgca cgccgccgaa gaggtgctga gcggcgccgg cgcgagcacc tcgcttttcc
     1561 gggcggccgc cgacgccgag atcgtcgacc ccttcacact cccaggaacg gccttcaagg
     1621 tcgagctcgc aaagcgcacg ctcgtgcgag cactggagac ggcggcggca tgacggcgct
     1681 gatcggctcc ggcgtcgacc gcgtcgacgg cccggccaag gtccgcggcg ccgcgcccta
     1741 cccgacagat gtcacgtacc cccagcttgc gcacgccgcg ctggtgcgca gcacgatcgc
     1801 gtccggccgg gtgatcgcga tcaacgacca cgatgcccgc gcgacagcag gcgtcctcac
     1861 ggtcatcacg cacgagacgg ctccgacgct gacacccgct cccttcgggc tgttgggccc
     1921 agccccgcca ccgcccctcc aggacgacca catccaccac cacggacagt acgtcgccgt
     1981 cgtcgttgca gcgaccgcgc acgaggccga cgttgcggcg cgcctggtca aggtcgaata
     2041 cgagggcggc gacccgccac tgctcgactt cgatgacccg cgcagcgcac tggaggccac
     2101 tccctgggac accgcgacac agcgcggaga tgtcgaagcc ggcctcgccg aggccgacgt
     2161 catccacgag gcgacgtaca ccactgccga caacaccaac aacccactcg ggttgttcgc
     2221 gacggtcgcc gcgtgggagg gcgacatcgt gacggtccac gacgcgacgc agtggcccgc
     2281 gtacacgcag gcgacgatcg ccgaggtctt cgggatcgaa cccgccgcgg tccacgtccg
     2341 ttcgccctat gtcggcggcg cgttcggggc cggtttgttc gtctggcagc acgtgattct
     2401 cgccgtcctg gccgcccgcg cggtcgggcg cccggtcaag ctcgtcttaa cccgcccgca
     2461 gatgttcacg ggtgttggcc accgcccgag cagcgtgcag tcgatccgga tgggggctcg
     2521 gcgcgacggc gagctcgtcg cgatcgagca cgtctcgacc aacagcgcgt cgatcaaaca
     2581 cgcgagcatc gagccgatca gcggtgccac cgcgcgctcg tacgcatgcg ccaacgtcgc
     2641 caccaccgac cagcagcggc gtctgaacat cccccttccg ggatcgatgc gcggtccggg
     2701 gcatgcccag ggcaacttcg cgctcgaatc cgcgatcgac gagctggcgt acgcgctcgg
     2761 cctcgacccg ctcgagctcc gccttcgcaa cttcgccgcg gtccacccgc aatcgggcct
     2821 gccgtggtca agcaacgcgc tgggcgagtg ctacacggtc ggggcagagc gcttcggctg
     2881 gtcacgccgc gacccggagc ccggatcgat gcgcgagggc cactggaaga tcggctacgg
     2941 cctcgccgcg attagctgga gctggtggca ggtccggtgc caagcgcgtg caaccatcac
     3001 tcgggacggc cgcgcccacg tgtgcagcgc cgcgagcgac cccggcaccg gcacccggac
     3061 ggtcatgcgc caggtggccg gcgagcggct agggcttccg ctcaacgacg tccgcttcga
     3121 gctcggcgac tccgagatgc cgtgggcgcc cgcgtccggc ggctcatcgc ttaccgcctc
     3181 gcttggaaac gccgtccacg cggcctgcga ggcgctcgtg cggcgattcc tcgacgttgt
     3241 cgctgacgac gaccggtcgc cgctgcgcgg ctgctcgctc gacgacgtgc gcgtcgccga
     3301 gggacgaatc caccgccgcg acgatccgtc ggacggcgag cattacaccg acatcctcgc
     3361 gcgacacggc ctcaacgagc tcaccgtcga cgccgaagcg acgccgccca gtcaggagaa
     3421 cgccgagctc gcccggtcag gcccgttcgc cgcgaagttc gtcgaggtcc gcgtcgacga
     3481 ggacctcggg ctcatccgcg tcgcacgcat caccacggcg atcgacgccg gtcgcatcct
     3541 caacgagaag accgctcgca gccagatcgt cggcggcacc atcggcggca tcgggcaggc
     3601 gctgctcgag gagacgatca ccgatcccgg caccgggcgg atcaccaacg caaccttcgg
     3661 cgactacctg atccccgtca acgccgacac gccggacatc gacgtcttct tcgtcgggcg
     3721 accggatcca ctcacgcccc tcggcgccaa gggcctaggc gagatcgggc tcgtcggcat
     3781 agcggccgct atcgcaaacg gcgtccacca cgccactggt cgccgcatcc gatcgatccc
     3841 gatcaccctc gaccgcctac tgtgagcgtg agaccgcctt gcgaggcttt tcgacgcgag
     3901 agctccataa acggctctat acccaccgca cgatcgacgc ggagcaaatc aatctgaacg
     3961 agaaagcgcc gacgtgcggc gctttcgcga agccctctga cggactcgaa ccgtcgaccc
     4021 cctccttacc atggaggtgc tctaccaact gagctaagag ggcgaggccg ctagggtagc
     4081 cgctttctgg cgcgcgagtt cccgcgtcct ggccgattgg caagccggtt ggcaagtgcg
     4141 aagaaacggc ccggccactg ggcggggtcg cgctcgagct gacctatcgc tctttgcacc
     4201 tcgacaccgg ggtgagcgct gcgaggctcc ggcttgccgc ggctgtccgt cacgctagtc
     4261 ggcggctgcg aggcgttcag gcggggtcgg gtgggtgaag aggagcaggt agagcaggcg
     4321 cggcggatcg aggtcagtga ggttcgtctg ggcgagtcgg cggaagacgg ttgcgtaggc
     4381 ggcgcggtcg ccggtgagct cgagcgagta gcggtcggcg gcgcgctccc agcgacggga
     4441 gatggccgtt agtgccggca gcgaggtgag agcgatcccg agcccgatga gaagcacgag
     4501 cggaacttgg tgcgggtcgc cgacgcgtgt gcccaggagt gcccagacaa cgaccgttgc
     4561 ggtcgctgcg cctgtcatcg cgagcagggt tccgagcagc acgtgccgct cgcgccggtg
     4621 agcgagctcg tgggcgacga cgagccgcac ttccgctggc gaagcctgtt cgaccagggt
     4681 gtcggacacc acgacccgcc gcgttcgcgc gagcccggac acgtacgcgt tcgccttccg
     4741 ggtgcgacgg ctcgtgtctt ggacgagcac gtcgcggatg gggacgccac cacgctccgc
     4801 gagtcggccc agctctgccg ccagggtcgc gtcctgcagc ggcgagtagc ggttgaacag
     4861 aggttcgagc acgaccggcg ccagaaacga aagcatgagt gtcgcgaacg cgaacgcggc
     4921 tgcggcgggc gctacccacc agcccggcag cgcacgggcg aacccgacca gcccaagcag
     4981 ggccacggcc gtgaggacga tgttgagccc gaccgccttg gcgcggtcgg ccacccacga
     5041 cggcaaccgc tgcgtcgaga acccccagcg ccgctcgcgg atgagcccgc tccacagagg
     5101 aagcggcgtc ctcacagccg tcgtcgcggc gaccacgatc gccgcgtatg caggcgtccg
     5161 accccaccac ggcagcgagc cgggatcgag cgcagcgccc gcgccgctcc acaccagcgt
     5221 ggccagcacg ctcgcctcga tcacaacgtc agcaatccct gcccaataca aaggacggtg
     5281 gtagcttcgc gcccgcgcca cctcctccgc cgagaagaaa gtcagcgcgt ctatccgcct
     5341 caatttcttt gctcactcga agggattggc tcgggccagc gatctagttg ttcaaacttt
     5401 cgcggtcaca gatcgtccgt cttcagtcgc gtcattcccg agggcgcttc ctcggcagcc
     5461 tcttcatcgg ccgtctcgat ggcttcctcg ccagaagacc gctcatccac cgccggctca
     5521 ctgcgagctt cctgctgatc gagcttacgc ctccagagct ttgcgcgtag accactgagc
     5581 acgaccgcgc ctcctcctga tcggacctcc ttatcgtact ctcgtacgct cggtgcggtc
     5641 cgccgacacc tcgccggcta atccgttgtc tagacaggca atcaccgcgg gctccggctc
     5701 cgacaccacc cgaccgcagt cgctccacgc gatcgcctac ttcgcagtat ggggttgcgt
     5761 cccggcggtt cctgacctca ataggaagag aagggctgcg cccagaagcg gggagaggac
     5821 gatcttctgg gcgcagccct agtttgacgc gttacgcaac ctcgagatag tcccgcagac
     5881 ccggcgcgag catccggagt ttgtcgagtg ctgtcgcctc gagctggcga gcccgctcgc
     5941 ttgtcactcc cagctccctc cccacctcga ccagcgaccg gggtgattgc cccgcgagac
     6001 cgaagcgcag cctgagtacg agccggaggc gctcgtcgag cgagttgacg gttgcctcaa
     6061 cctcctcttt tcgctggcgc tcggccagtg ccgcgtccag gtgggctgat gtcggatccg
     6121 cgagcagctc actgcaagcc tgctgcccct ctttcggagg ggactcgaaa cttacgggct
     6181 gctgctcgta gccgagtagc tctccgacgc ggcgggaatc gagtcctgtc tcggcagcga
     6241 tctcacacag caaggggtcg cgattcaggc gctccaggag aacctgacgc gctcttcgaa
     6301 cggtgcggag ctgcctcccg acgtggattg gaaccggaac caaacgtcca tgacattctg
     6361 cagctcgccc gaccgccccc ttgatcatca aggtggcata ggtcgagagc ctgaaaccca
     6421 atcggtgatc gaacctctcg accgccctcg tgagtgccag gttcccttcc tggatgaggt
     6481 cgagcaacgg aacgttgtga tgcgcgtact tacgggcgat ccagatgacc agacgcagat
     6541 tggcctcgat cagaatccgc ttcgcacgct cgtctccggc ttccttacga cgaaagagct
     6601 cgcgctcttc agccggagac agcatcggta cccggcgtac tccgcgggcg taaagcccaa
     6661 gtgagtcctc gagctcgtag ctcgagtcaa cctcgcttga tgtcctgtcg tccgttcgat
     6721 cgaactgccg gttcataagg tcgatgctca tttggaacct cctttcgcac tcgagctcgt
     6781 ctcgacacag ggggcacccg aggcgccccg ccgtgcgcgg atgaccgttc gtcaggcctt
     6841 agccaggacg gtcgagcaag aacgcgagag gcactgcgcg cgggcacagc tcaatgagcg
     6901 tgattggacg cgtgccatca cctggagtga tgttgaatac cgccgctgcc acaccgagag
     6961 tggacccgcc gtgggggcct cctgggctgc ctgatccgct cgaggccccc gctggggttg
     7021 gcatctgtgg ctctttgtcc gggatgggta ggcggggagc tgatggcccg cgcgcgtcgg
     7081 ccggccgccg attctgcgcg ccgttcgtcc tcaccgctat gcgtgacgcc cgcgtcgccc
     7141 cgggtttggt gaagcatgaa gcgagaggaa gagagcgaag ggtcgtgtcg caaacgaggg
     7201 tgatgcggca gtcggcaccg cagcggatca caatgaccga ggagatgagc gtcgtggcgt
     7261 cgccgggata cacgtccgtg gcgaacggct ggacaacgtc gccggtgagg aggtccgcgt
     7321 cggcgtcttc gtcatgcgac gtggcgtcgg acagaacgtc gctcgtagtc gcatcactcg
     7381 gggcctgcaa attcgagcct ggcgggggag caggtggttc cgcagctccg gacaagcccg
     7441 agctcgagag cgatcgcgtg tccgtggcgg gcgcagtacc cgttgacgtg cctatcggct
     7501 cgttcgtggt ggtgaaggag tcggcagccg catccgtagg ttcggcgccg gaagcgtcgg
     7561 tggaactggc ggagggatct gtcgcagtga ggccgtcagt cgtgtctggt acgactggtg
     7621 ccggcgcaca cgcgccagtc gtgtctgttg cagcggcgga agtgtccgtc gtcgtggccg
     7681 aggcggctgt tgcgtctggc accaaggcgc acgtgctggt cgtgtctggc gcagcggcgg
     7741 agttgtctgt cgttgtggct ggggtggcaa ttgtgtctgg agtcgtcgcg gcggaggtgt
     7801 caattgcgtt tggtgttgtc gtggctgtcg tcgccgcgga cgtgtccgag gtttctgtcg
     7861 gagcgatcac gtccgacgat gccgttgcgg cggcgtcgct cgacgtcgag tctgtcgtca
     7921 tcgccgagcc atccgggacc ggcgaaggag gaacgtcgcc tcgagccggc gcggtgaata
     7981 gggacgcgca gagcacggcc gcaaatacgg ctaccgcgca accgacaaac cctcgccctc
     8041 ggttacgctc aaaacgaagt ggccgcattc ccgaaacggc ggccgagctc ccctccatca
     8101 gcgcttactc cccatggctg cgccccttgt ccgagcttcg agtatcgcac ttgacatcag
     8161 gtgcgcaagg ttgcgacgcc gacgggagag ccggcaggaa cgatggccgg tctcctttgg
     8221 gggggtagct caacctcaag tggagattga tcgttgtgct actcggtagc ccgggtagcg
     8281 gcggaccccc gtggaactag gaggccccga atgcgtaccg ccaaaactcg cgcatgaatt
     8341 gtgtcggcgt cggccccgtc atctggagct gcgtgaggag gatgccgatc gtgccgctcg
     8401 aggacgcgac gtgtgcgctc gtgccggtgc cgccgaccca gccgtagcgg ccgtcggctg
     8461 cgacggcgcc gccgaagccc cagcctgctc cctcgaggaa caacgtcgac gcgtcgcgct
     8521 gctcctgcgt gagatggtcg gtcgttatca agcgcaccga ctcgggcgat aggagcgcgc
     8581 cgccgtcggc gagcagcatg cgcccgaagc ggtgccagtc ggagagcgtc gagacgagtc
     8641 cgccggcgcc cgatgggaag atcggcggct ccgtccagag cccgtcgtcg atcggcgcga
     8701 agtcgggtcc gcggtatggc gggagccggt cgagcttctc cgaggggacg tggaagctgg
     8761 tgtcgctcat cccgagtggc tcgaagatgc gctcggcgag gaattccggc agcggctgcc
     8821 tcgagacgcg cgcgatcaag acgccctgga cgtcggagca ggtgttgtag agccacgcct
     8881 cgccgggctg gcgcagcatc ggcacctgcg cgagcgtcgc gagccactcg tcaggcgtgt
     8941 ctcgcggccc gaacaccggc agcttccgaa aaagctcgac cacagccggc agcgagaagt
     9001 cagagggaaa accccagccg gcacggaaag tcaagacgtc ctcgacggcg attgggtgcg
     9061 tggcaggcac gacgtcgtcg atcgcgctct cgggggtccg cacgaccagc ggcgacgcca
     9121 gttcgggcag ccagcgcgcg atcgggtcgt cgagcgaaac gagcccgtcg tccaccagga
     9181 tcatgatcgc tgcagcggtg atcggcttcg tgatcgaggc gatccgaacg atcgactccc
     9241 tctcgacctc acccgcctca gcgacttcga cgtcgtcacc acgcgcaacg agtgcggcgg
     9301 cgcccggcac cgtgcccctt tcaacggacg tctcgagcaa gtcgcgcagc ggcgtcatcg
     9361 cggatacaga tcctgccagc ggccgagact catcggcgat gagtttccgt cgatccagcc
     9421 gtctaagaga caaactccga caaaaaggag gcccagatgt cgaaagtggc atccaagctc
     9481 aatctcacgc aggtcggccg cgtctgcgtc accgtcggcg acacggatcg cgcgatcgag
     9541 ttctacgtcg acacgctcgg cttcgaaaag gtggtcgacg tcccgatggg cccggagatg
     9601 cgctgggtcg aggtcgcact ggccggaacg ccgacgacga tcgccctcgc gccgccgccg
     9661 caggacgagg aagcaggcgg cagccagacg ggaatctgcc tcgacaccac cgacgtcgat
     9721 gccgaccacg ccgcactcaa ggccgccggc gtcgacgtcg accagcaggt cacccggtgg
     9781 ggcggcccgg taccgccgat gttctggctg cgcgacccgg acggaaactc gctcatcatc
     9841 gtccagccgt cggactagcc cctcgccgtg actgtgcgac cggcgcaccg ccgccggtcg
     9901 cgcaccactt caaacggaac tcgatgccgt gctgcggggc ggccagcggt cgctgctgac
     9961 gaaggagcac gaacgccctt gtcgtcgcgt gcggctacgt gaatccaatt gacgatctcg
    10021 tccgacccgg agctgatgtc taccgatgcc ggacgtaaac gcacgcgcgc ggtcggggct
    10081 gtgggggagg gcccgggaag cgaggcttca gaagaaccga ccgagagctg cggcacccca
    10141 cggccggaca tccgggcctc cccagaaaga aaggttcgtc tcgacgggcc gggccgacta
    10201 cccccaacgg ggttaacttc aacctcctaa aagctccacc gaactcgcaa aaacctgttc
    10261 aaggctcatc tggctccgtt gggtgcgcca atcaaccgga tgggggacga atcaacccgc
    10321 cgccttgacc gatgttctgc acgagcgtgg tggggctcga tgcggcggag cctcccgctc
    10381 agcaaagggt catcgtgagc agagtccggc cacgcggctc tgctcagggg ggtcgagggt
    10441 tggtcggctt tagctgcggt cgaccactag cttcagcgtg tcacagggtg tctgagggtt
    10501 ctgtcgctat ttgcgaagag aagccgttga caactctttt ggtaggggcg cacactctag
    10561 gggcgcacag tgctgagcgc tatgggcggc ggagcggccg cctgaatcgg ctggggggag
    10621 agccgtcctg cggtttcaat aagtcgcttc gctgctcttt tcggcggaag ccgcaaggag
    10681 aggagcatcg gaactcggaa gcttgttccg cgcgacatcg tggcagggct cgttcccgat
    10741 cagccgaggc tatctcctcg cttagtgcct tgaaggactg ccggtatgcg ttcggcggtt
    10801 ccgcagcgcc gggcgcgctc taagccggca ggaaggggcg gcgcgctgga gtctccccgc
    10861 cggtgcgccg tcccgagaac ctgaggcctg agccgcactc ggtcgaagct gttagacccg
    10921 ttcgaaaacc gactcgagcg gaaagtctgc aaggtgtgat ggcggtaagg cggcactcga
    10981 ggagttcacg gagcttcgct ggataaccat ccagaacagc ccgcgccaat acccgatcta
    11041 gccgtccccg atcgcccgca cttgacgagg aggcgcgact atcctcgcgg catgcagact
    11101 gttgccgtcg tcggagccgg gccgaagggc atcgccctcg cggccaaggc gcgagcgttg
    11161 gccgacgtcg gattcgacgc cccgcaggtt gtcctgatcg accgcgccgg catcgccgag
    11221 agctggaccg gacggcgtgg atacacgaac ggcctcctac cgctgggcac gccgcccgag
    11281 aaggacgtcg gtttcccgta tgccgagagc tggggtggcg cctcggccga ggtccgcgac
    11341 gccatgacgc ggttcagctg gcagcaccat ctgatcgagc gcggcgagta cgccgactgg
    11401 gtggaccgcg gtcatttgcg tccgtcccac cggcgctgga gcacctatct gcgcgcggtc
    11461 gcgcagaagg ccgaggccga tgtgatcgag gccgacgtcg tcggactgga gctcgaagac
    11521 gaacgctggc gtctgtcgct cgccgacggc gcggcgctcg tcgccgacgg cgtcgtgctc
    11581 accggcgccg gcccggcgat cgaaatcgcc ggccagcccc gcgggcaccc gcgtgtgctc
    11641 gacggccgga gctactggct acacgagaag gagttcaaga aacacttcgg cttgaacgtc
    11701 tgtgtcatcg gcagcggcga gactgccgca tcgatcgtga tcagcctgct gaagaagctg
    11761 tacaaagggt ccacggtcga cgtgttgacg agtcggggcg tgctctactc gcgcggtgag
    11821 agcttcgacg aaaaccgttt ctattctgat cccggcgact ggccaaggct ggccgagacg
    11881 caccggcgcg agttcctgga gcgcaccgac cgcggggtct tttcgctgca ggccgaggca
    11941 accctgaacc aatcgcgctg cttccgcacc ctcgccgggc gtgctgttgc aatcgacccg
    12001 ggcggccgcc aggtgttggt aacgatcgag tacggcgatg agcaggaacg cgtcgtctac
    12061 gacctcgtcg ttgtggcgat cgggttcgag gctcgctggt tcgagagctt cctcggccgc
    12121 gaggcaagcg cacgcctcgt cgacgccctc gccgacgacg agctcgagcg gcgcatcgac
    12181 atcgacctcg gcgtcgatgg actccggccg cgcctgcatc taccaatgct ggccggcctg
    12241 gcccaagggc ccggtttccc gaacttgagc tgcctcggac tcttgagcga ccgtgtcctg
    12301 cgcagctacg tcgacggcct cacgccgcga acgcccgatc agctggggga gaggaggcag
    12361 cttgtctgac acgcaagatg aggtctggga gagcgacgag ctcgttcgcc ggacgatcgc
    12421 cattccacgc cggctcgccg agcgcctcgc cgctgaggcc gagcgccgcg atctgggcat
    12481 caacgatctg atcgtcgaac tcgcggagga ggggctaccc gagcccgctg cggacgattg
    12541 aggttgacgc gcgcgcgcga ataccagata tcccgccgcg gcgcaaggtt gcttgcggcg
    12601 agcagttgcc tgagctacga gcgcgatgct gcggtagagg cagccgccgt ggcgacgtca
    12661 cgcgggtagg tgcgcaaggc cctgaagagg aagtacgcgc tgccggcgag tggaagcagc
    12721 atgaacgcga aggtccactg caggcccgac cggccgccgc cgaacaggta gtccgagagc
    12781 gccccgaaca acagcggcgc agtggcctgc gcgagcgtgc ggagggccgt gcggatgcct
    12841 tcggccctgc cccagaggag gggatgaacg atgtcgaggc gcgcggcatc gatgggcggg
    12901 ttttgcgcgg agagggcgag tgcggcgagc gagatgtagg gcagcgccgt cagcgggctt
    12961 cggatgaaga tcgccggcac aaagaggatg acggccacag tcgcagcgat cgcgctcacg
    13021 aggatccggc cgttgaggaa gccgcgatgc aacaggaagt cgcctagcca gccgcctagg
    13081 aggactccga tgaccccggc ggcgccaacg gcgaggagta ccagcgcgct gagcgcctga
    13141 gcgattccgt actgcttcgt cacgaactcg acgccgaagg tctggactcc cgagaggaag
    13201 aagtagccac cggcgctcgc caagatgagt aggacgttcg tcctgatcga gagcacgtac
    13261 cgcgcggcgg cgagcagtcc cagccggcgc gggtcccggg tcaacacgag ctgcagatcc
    13321 ggttcgacgc cgcgttcgcc ggcgaggcgc tgcgcatcgg tcgcctggtc ctcgccgtcg
    13381 tcgccggcag cggcaggaac cggcgtcgcc gttgcagata ggagcggctt ccccccgccc
    13441 cgggtcggct ccggcagatg gaagacgagc cacgcgagtg cgaacgccgg cagggcgagg
    13501 atcacgaacg aagcccgcca ggacacggtc gcgatcccac ctgtgaccgc gaagccgaag
    13561 gcggcgccga cgagctctcc cgagatgatg tagccgtaga tgcgccctcg ctcggaggag
    13621 gcgaaccagt cgccgacgag cgacgcgacg accggcccag ccgccgccgt gacgccgccg
    13681 aggaagaggc gtgcgaggag cagctgaccg aacgtgggcg cggtagcgct ccagatcatt
    13741 gcggcgcccc agagcacgat cgcgccgccg agcaatcgcg tccgccgcac gcggtcggcg
    13801 aggacgccga agggaaggct cgccgctgct gcgacgagcg acgtaacggt gaccagcagc
    13861 ccgacgtccg tgttgttgat gtggaggtcg tgccggagtt ggatcgccga tgctccaacg
    13921 gtggcggagt cggcactcgc gagtgccaag accgacgcca gcaagaggat cacgcgggtt
    13981 cgctccctgc ctccgagcgt ggtcgtcaaa gcattcgagc ctgcgcgagc gagccggccg
    14041 gccgcggcca cggcgatatt gctcggccgg cggcgtgtgt tcccattgcc tgtcatcgag
    14101 gcagggtcac cgccagcgat cgtaggcttg acccctcctt cggagcgggg gaaggatgac
    14161 gctacggccc gcgtggcctg acgcgactcg tagaagcggt caggtccggc gccgaagccg
    14221 gtcttcgtat tcccgaggcc ggtggtaata ggcggcgagg agcgctaccg ccattagcgt
    14281 cgcgatcagc gccgtgttgg gatcggtcgc ctgcccggta tagagctggc cgaagtcctg
    14341 tccgaccacc cagatcgcga gcaaggcgag gagcccggcg gcggcgctga cggccaccgt
    14401 cctgcgggac agggcggtga gcccgatcag cgcctcgacc gcgacgagca cgacgacggt
    14461 cgcaagccca tggtgactga cccagccggc gagcgaggct tggaacgacg cgagccagct
    14521 ggggccgccg aaggcgagga gggcgctc
//
LOCUS       DASVEU010000169         9859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_894376,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000169 DASVEU010000000
VERSION     DASVEU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894376"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1187)
                     /gene="trpD"
                     /locus_tag="VF225_06800"
     CDS             complement(162..1187)
                     /gene="trpD"
                     /locus_tag="VF225_06800"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7145002.1"
                     /translation="MIQEVIGKLLDGTQLARAEARAVMDEIMRGEATPAQIGGFLVAL
                     RSKGETADEITGCAEAMRDHVLVVRPDRDDLVDTAGTGGDGAHTINISTAAALVAAAA
                     GAGVAKHGNRAVSSACGSADVLEALGFALAQEPQRIARSIDELGFGFLFAPSHHPAMR
                     HAAAVRRELATRTVFNVLGPLTNPAGARAQVVGVYSPGLVRTIADVLARLGARRAYVV
                     HGEGGIDELSPVGQNLVAEVVDGEVLERRLDPESELGIPRCSVDELRGGSPAENAEAI
                     REVFAGAGGGRRDAILLNAAGAIAAGGHAADLREGLEVARRTIDSGAAAERLELLIAF
                     SRETVAA"
     gene            complement(1184..1741)
                     /locus_tag="VF225_06805"
     CDS             complement(1184..1741)
                     /locus_tag="VF225_06805"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7145003.1"
                     /translation="MILLVDNYDSFTYNLAHLFAELGADVTVRRNDAIDADTAERLSP
                     SHLVISPGPGRPADAGSTPEIVRRLALTTPTLGVCLGHQAIVEAFGGEVAAARELVHG
                     KSCTVNHDGRGVFVGLPQELEVGRYHSLAATAVAAELEICATAADGEIMAVRHRTLNV
                     HGVQFHPESVLTPLGREIARNFLNQ"
     gene            complement(1738..2736)
                     /locus_tag="VF225_06810"
     CDS             complement(1738..2736)
                     /locus_tag="VF225_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX7145004.1"
                     /translation="MPLPHEGTGLAESRFVVADVLIRFDHVLGSAEVIRGDPAALSSL
                     LAQPVPALPRLHGRRNSARRTPGRAEYEAWVRSAKEHIRAGDAFQIVLSQRAERRTSA
                     SALELYRALRRVNPSPYLFLLELDDLALIGSSPETLVKCEGRRAGVNPIAGTTERGEG
                     DAERLLASEKDRAEHVMLVDLGRNDLSRVCVPGTVEVEKFLQVERYSHVTHLVSQVAG
                     DLRDDVTSFDLLRACFPAGTVSGAPKVRAMQLISELEGRRRGPYAGAVGYALPEESTL
                     DTCIAIRTIVLRDGVAHLQAGAGIVADSEPAAEHEECLRKLAAVEAAIDLAEADQR"
     gene            2722..2991
                     /locus_tag="VF225_06815"
     CDS             2722..2991
                     /locus_tag="VF225_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145005.1"
                     /translation="MRKRHGRFQARGVVVREIADDRLSARLCLCKIAEPRAAADERVS
                     SEPSAFDRLEQEGCTAFPAQSQVGAERGDEIGGDVGCDGYLEILR"
     gene            complement(3125..3796)
                     /locus_tag="VF225_06820"
     CDS             complement(3125..3796)
                     /locus_tag="VF225_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7145006.1"
                     /translation="MPTQHTTVKRHPERGAYDRATIDSILDEALICHLGFVADGRPFV
                     IPTIHARDGDTLYVHGSPGSRMLRAAKEGVDICVTVTLLDGLVLARSVYNHSMNYHSV
                     VVLGRALELTDRDEKLRAMQRVVEHVVPGRWDDARRPNDGEIKGTTILALPLDEASAK
                     IRSGPPTDDAADLELSVWAGVIPLRIEPSEPVPATAVDSPPPSYATGYVRLTPAEAAP
                     TLRPR"
     gene            3825..4976
                     /locus_tag="VF225_06825"
     CDS             3825..4976
                     /locus_tag="VF225_06825"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7145007.1"
                     /translation="MARSVIVSAVRTPFGKLGGSLADKQATELGAIAIRAALDRAGVG
                     NDEVEYVIMGQVLQGGVGQAPARQASIGAGLPIEVGSDTINKVCASSIRAVEIADQMI
                     RAGDSQVVVAGGMESMSNAPYALKKARFGYRLGDGTLIDLMTHDGLTSTFDNKHMVEQ
                     ASFVSRELGIPREDQDRWALRSHQRAVDAIDHGKFNEEIVPVGEFAVDEGPRRDTSYE
                     KLARLRPAFDPDGTTTAGNAPGVNDGAGALIVTSEEFARKRGLEVLATIISQAYVADD
                     FAYLARTPAKAGAAALAKAGKKIGDVERVELNEAFSSVVLNSAKMLEVDPEKVNVNGG
                     AVALGHPIGASGARILGTMIYELRRNGGGLGVAAICSGGGQGDALLLEV"
     gene            5006..5149
                     /locus_tag="VF225_06830"
     CDS             5006..5149
                     /locus_tag="VF225_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145008.1"
                     /translation="MPRTFVVLLGGLAVAVLVYVLSGGHVFFLPLILLLPFGLFIGRR
                     RRS"
     gene            5168..6001
                     /locus_tag="VF225_06835"
     CDS             5168..6001
                     /locus_tag="VF225_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7145009.1"
                     /translation="MKFAHVLVVGAGQMGGGIAQVVAASGRRVSLHDAQPGATDRALD
                     TMLRSLAKLEEKGGPAAQEVFARVEPVEDLVQADLLIEAVVEDAEVKEEIFRRADAEL
                     PVEAVLASNTSSIPIGSLAAATGRPDRVIGMHFFNPVPVLKLVEIIRGRDTSDETAAA
                     ITALARELGKTPAVANDFPGFVSNRILMPFINEAVWALHDGVAEAEAIDTIARLGFAH
                     PLGPLALADLIGLDTCTAIMEVLRDGLGDDRYAPCPLLRELVVAGKLGRKSGSGFYEY
                     S"
     gene            6130..7209
                     /locus_tag="VF225_06840"
     CDS             6130..7209
                     /locus_tag="VF225_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7145010.1"
                     /translation="MRILLVDDDPALRTLLKTTFEVADVAVVEAEDAGAARRKIKSAR
                     PDVIVLDINMPGTTGLELSAELKAAPATRDIPIVLLTGSEGGTEVAAKKAGANAFVRK
                     PFSPLELLAVAERLAGGGLFGVPFRAAEKRTQGPDEELLLYARDLRHMLGVERAQREL
                     LQSAYLQTVSALAGALESKDTGTRAHSQRVQSYATALAQAVGEGALVRDESAPYGFLL
                     HDVGKIGIPDGILQKPGPLSPAERRKMQTHTVLGEAMLSGVVFLKGEGLKIVRSHHER
                     WDGRGYPDGLVHDEIPLGARIFAVADALDAMTSHRPYRRAMSWDAAHAEILEQRKRQF
                     DPDVVDAFVMVEPALREIRRELAAA"
     gene            7268..8431
                     /locus_tag="VF225_06845"
     CDS             7268..8431
                     /locus_tag="VF225_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145011.1"
                     /translation="MDFDLTQDQREIQALARDFARAEIDPHAAHWDREHHFPRELFGK
                     LAELGLMGVCVPEEYGGAGADFLSYVLVLEELSRADAGVGVTVAVHTSAATLPILAYG
                     TDEQMSRFVPPLARGEQLGAFALTEAEAGSDAGALRTRAEAATDGWTITGAKQWITNG
                     RHAGTFILFARTDPEVAGARGVSAFILDAEHVRVTRDEEKLGLNSSVTSDIVVEGAQV
                     GRDRLLHEQNRGFRVAMGTLDGGRIGIGAQALGIAQAGYEVAREYAAERRAFGHRIAD
                     FQAIQHKLANMSMEIDAARLLVYRAAWLKEQGRRHTEEGAKAKLFASEMARRQTAEAI
                     QILGGYGYTKEFPVERYYRDAKITEIYEGTSEIQRLVIARSILGTTERSLVAG"
     gene            8574..9155
                     /locus_tag="VF225_06850"
     CDS             8574..9155
                     /locus_tag="VF225_06850"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7145012.1"
                     /translation="MSPRRRDEPVRLTRIYTRAGDGGETSLGDGSRVPKLDCRIGAFG
                     TVDELNAALGVVLTAAGLQEHLRAPLSRIQNDLFDVGADLSVPFGVTDRLRVEQAAVD
                     RLEQLCDELNAELPMLTSFILPGGTEAAARLHVARTTCRRAERDALAANEENEINPLV
                     LAYLNRLSDFLFIAARSANADAGRDEPLWKPGG"
     gene            complement(9227..>9859)
                     /locus_tag="VF225_06855"
     CDS             complement(9227..>9859)
                     /locus_tag="VF225_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145013.1"
                     /translation="AVLKKKIAARISVTRASGVTAELFSPRHVKLYTWRFSLKAGRSI
                     VKLRLPHQVRRSGAYTMRWTARSGRESISHKLTIRLVGANNVIAVPARVLLAGPAAGG
                     IRGALTTQKPKLITASGVEPTFDAAASRRTDVRVIVVDVDAFGVSLIRDLHAVFPSTK
                     IVALTSGPKQMAASLKAGAAIALPRSTPASTLGRLIQRLLAKPAKPVKVA"
BASE COUNT         1612 a         3215 c         3466 g         1566 t
ORIGIN      
        1 gacgaggatc gagatcgcgg ccgcgcctgc gttcgcgaac tgcgcggcga gccgcgcggg
       61 gtcggcgtcg gggcgaaggt ctcccgcgga cggcgagcgg cgcttcacct ctgcgatcgc
      121 ggcgagcccg gaggcggaga gagcgtcgcg gaaacggccc atcaggctgc cacggtctcc
      181 cgcgagaacg cgatcagcaa ctcgagccgt tccgccgctg cgcccgagtc gatcgtccgc
      241 cgcgccacct cgaggccttc gcgcaggtcg gcggcgtgcc cgcctgccgc gatcgccccg
      301 gcggcgttga gcaagattgc atcgcgtcgc ccaccgcctg cgccggcaaa gacctcccgg
      361 atcgcctccg cgttttccgc gggactcccg ccgcggagct cgtcgacgct gcagcgcggg
      421 atgcctagct cgctttccgg atcgagccgg cgctccagca cctcgccgtc cacgacctcg
      481 gcgacgaggt tctggccgac cggtgagagc tcgtcgatgc ccccttcacc atgtacgaca
      541 taagcgcggc gagcgcccaa acgggcgagc acgtcggcga tcgttcgcac gaggccgggg
      601 gagtagacgc cgacgacctg ggctcgcgcg cccgcgggat tcgtcaacgg gccgagcacg
      661 ttgaacaccg tcctcgtcgc gagctcccta cgcaccgccg cggcgtgtcg catcgcagga
      721 tggtgtgaag gagcgaacag aaagccgaac ccgagctcgt cgatcgacct cgcaattcgc
      781 tgcggctctt gcgcgagcgc gaagcccagc gcttcgagga cgtcggcgga cccgcacgca
      841 gatgagacgg cgcggttgcc gtgcttcgcc actccggcgc cggcggcggc ggcgacgagc
      901 gcggcggcgg tcgagatgtt gatggtatgg gctccgtcac cgccggtgcc cgcggtgtcc
      961 acgaggtcgt cgcggtccgg ccgaacgacg aggacgtggt cgcgcatcgc ctcggcgcaa
     1021 cctgtgatct cgtcggccgt ctcgcctttc gagcgcagtg cgaccagaaa tcctccgatc
     1081 tgcgccggcg tcgcctcgcc gcgcatgatt tcgtccatca ctgcgcgtgc ttccgcacgc
     1141 gccaactgag taccgtcgag cagcttgccg atgacttcct ggatcactgg ttcaggaagt
     1201 ttcgcgcgat ttcgcgcccc agtggggtga ggacggattc ggggtggaac tgcacgccgt
     1261 ggacgttcaa ggtgcgatgg cgaacagcca tgatttcacc gtccgcggcg gtggcgcaga
     1321 tctccagctc ggccgcgacg gcagtcgcgg ccagggagtg gtaccgcccg acctcgagct
     1381 cctgcgggag cccgacgaag acgcctcgtc cgtcgtgatt cacggtgcag gacttgccgt
     1441 ggacgagctc ccgggcggct gcgacctcgc cgccgaacgc ctcgacgatc gcctggtgcc
     1501 cgaggcagac cccgagcgtc ggcgtggtga gcgcgaggcg ccgcacgatc tccggcgtcg
     1561 agcctgcgtc tgcaggacgg cccggaccgg gtgagatcac caggtgcgac ggcgagagtc
     1621 gttccgcggt gtcggcgtcg atcgcatcat tacggcgcac ggtgacgtcg gcgccgagct
     1681 cggcgaacag atgcgcgagg ttgtaggtga aggagtcgta gttgtcgacg agcaggatca
     1741 ccgctgatcc gcctccgcga gatcgatcgc cgcttcgacg gccgcgagct tgcgcaggca
     1801 ctcctcgtgc tcggcggcgg gctcggaatc ggcgacgatt ccggctccgg cctgcagatg
     1861 cgcgactcca tctcgaagga cgatcgtcct gatcgcgatg caggtatcga gcgtgctttc
     1921 ttctggcagg gcgtagccga ccgcgcctgc gtacgggcct cgtcgcctgc cctcgagctc
     1981 cgagatcagc tgcatcgcgc gcaccttcgg cgcaccggac accgttcccg ccgggaagca
     2041 ggcgcgcagg aggtcgaacg atgtcacgtc gtcgcgaagg tcgccggcca cctgcgagac
     2101 gagatgcgtg acgtgcgagt accgctccac ttggaggaac ttctcgacct ccacggtgcc
     2161 gggcacacag acgcgcgaga gatcgttgcg acccaggtcg acgagcatca cgtgctcggc
     2221 ccggtccttc tcggacgcga gcagccgttc tgcatcgcct tcgcctcgtt cggtcgtccc
     2281 ggcgatcggg ttgactccgg cgcgacggcc ttcgcacttg accagcgtct cgggggacga
     2341 cccgatcagc gcgaggtcgt cgagctcgag caggaagagg tacggcgacg ggttgacacg
     2401 ccgtaaggcg cgatacagct cgagcgcaga ggccgaggtg cgtcgctccg cacgttggga
     2461 gagcacgatc tggaacgcgt cgcccgcgcg gatgtgttcc ttggccgagc gcacccatgc
     2521 ttcgtattcc gctcggcctg gagtacgtcg ggccgagttg cgccgaccgt gcaagcgcgg
     2581 aagagccggg accggctggg caagcagcga cgacagcgca gcagggtccc cgcggatcac
     2641 ctctgcgctg cccaggacgt ggtcgaacct gatcaggacg tccgcgacga caaaacgact
     2701 ctcggccaga cccgtccctt cgtgcggaag aggcacggtc ggttccaggc gcgcggcgta
     2761 gtcgtacgag agatagccga cgaccggctc tccgcacgtc tctgcctctg caagatcgca
     2821 gagccgcgtg ccgcagccga cgagcgagtg tcgtccgagc cgtccgcgtt cgaccgactc
     2881 gagcaggaag gctgcacggc cttcccggcg cagtctcagg taggcgccga gaggggtgac
     2941 gagatcggtg gcgatgtcgg gtgcgacggc tatctcgaga ttctccgttg aatcgacggg
     3001 cgtacaaaaa gaccttccgg tggaaggtct cggtgagcgg aaacgggctg taccggccta
     3061 ggtcagcgca ggcgcaggct cccgctccgc tcgcaacgcc aggcccagat gcgagttgcg
     3121 ggagtcatcg tggcctcagg gtaggcgcag cctcggccgg ggtcaagcgg acatagccgg
     3181 ttgcgtatga gggcggaggg ctgtcgacgg ccgtggccgg cacgggttcc gacggctcga
     3241 tcctcagggg gattacgccc gcccagacgg agagctcgag gtcggcggcg tcgtcggtcg
     3301 gcgggccgct gcggatcttt gccgacgcct cgtcgagcgg cagcgcgaga atcgtcgtgc
     3361 ctttgatctc cccgtcattc ggccggcgtg cgtcatccca gcgtccggga acgacgtgct
     3421 cgacgacgcg ctgcatcgcc cgaagcttct cgtcacggtc ggtcagctcg agcgcgcgtc
     3481 cgagcacgac gaccgagtgg tagttcatgg agtggttgta aaccgagcga gcgagcacga
     3541 gcccgtcgag gagcgtcacg gtcacgcaga tgtcgactcc ttccttcgcg gctctgagca
     3601 tgcggctccc cggtgagccg tggacgtaca gcgtgtcgcc gtcgcgggcg tgaatcgtcg
     3661 ggatgacgaa cggacggccg tcggcgacga agccgagatg gcagatcaac gcctcgtcga
     3721 gaatcgagtc gatcgtcgcg cggtcgtagg cgccgcgctc cggatgccgc ttcacggttg
     3781 tgtgctgcgt cggcatacct gaagcctatt ggtacgctcg aaccatggct cggtcggtga
     3841 tcgtctccgc agttcgaact ccgtttggca agctcggcgg ctcgctcgcc gacaagcagg
     3901 cgacggagct cggcgcgatc gcgatccgcg ctgcgctcga ccgcgccggc gtcgggaacg
     3961 acgaggtcga gtacgtgatc atgggccagg tccttcaggg tggagtcggc caggcgcccg
     4021 ctcgtcaggc ttcgatcggc gcaggactgc cgatcgaggt cggctcggac acgatcaaca
     4081 aggtctgtgc atcctcgatc cgtgccgtcg agatcgccga tcagatgatc cgggccggcg
     4141 atagccaggt cgtcgttgcc ggtgggatgg aatcgatgtc caacgcgcca tacgcactga
     4201 agaaggcgcg tttcggctac aggctcgggg acgggaccct gatcgatctg atgacgcacg
     4261 acgggctcac gtccaccttc gacaacaagc acatggtcga gcaggcgtca ttcgtctcgc
     4321 gcgagctcgg catcccgcgc gaggatcagg accggtgggc gctccgctcg caccagcggg
     4381 cggtcgacgc gatcgaccac ggcaagttca acgaggagat cgttcctgtc ggcgagttcg
     4441 ccgtcgacga aggcccgcgc cgcgatacgt cctacgagaa gctcgccagg ctgaggccgg
     4501 cgttcgatcc ggacgggacg acgaccgccg gcaatgcacc cggtgtgaat gacggagcgg
     4561 gtgcgctcat cgtgacgagc gaagagttcg cgcggaagcg tgggctcgag gtgcttgcga
     4621 cgatcatctc gcaggcctac gtcgccgacg acttcgcata cctggcgcgc acgcccgcta
     4681 aagcgggagc ggctgccctc gcaaaggccg gcaagaagat cggcgacgtc gagcgcgtcg
     4741 agctcaacga agcgttctcc tcggtggtgc tcaactcggc caagatgctc gaggtcgacc
     4801 ctgagaaggt gaacgtgaac gggggcgcag ttgcgctggg gcacccgatc ggggcgtcgg
     4861 gcgcccgcat tctcggcacg atgatctacg agcttcgccg caacggcggc ggccttggcg
     4921 tggcggccat ctgctccggc ggcggccagg gagatgcact gttgctcgag gtgtaggtga
     4981 cgtcgcgggt ggtgacatcg cgaccatgcc tcgcacgttc gtagtgctgc tcggcgggct
     5041 cgccgtcgcg gtgctcgtct acgtcctttc gggcggccac gtgttctttc tgccgctgat
     5101 cctgctcctt ccgttcgggc tcttcatcgg acgccgccgg cgttcgtagc ggcgtaggct
     5161 cgccgacatg aaattcgcgc acgtgctcgt ggtgggagcg ggccagatgg gcggaggcat
     5221 cgcccaggtc gtcgcggcat cagggcgacg tgtctcgctc cacgatgcgc agccgggtgc
     5281 gacggaccgc gcgctcgaca cgatgctcag gagccttgcg aagctcgaag agaaaggcgg
     5341 cccggccgcg caggaggtgt tcgcgcgagt cgagcccgtc gaggatctcg tccaggccga
     5401 cctcttgatc gaagcagtgg tcgaggacgc cgaggtgaaa gaggagatct tccggcgggc
     5461 cgatgcagag ctgccggtgg aagcggtgct cgcttcgaac acgtcgtcca ttcccatcgg
     5521 gtcgctggct gctgcgactg gacgtcccga cagggtgatc gggatgcatt tcttcaatcc
     5581 ggtgcccgtc ctgaaactcg ttgagatcat ccgcggccgg gacacctcgg acgagaccgc
     5641 tgcggcgatc actgcgctgg cgcgcgaact gggtaagacg cctgccgttg cgaacgactt
     5701 tccgggcttc gtttccaacc gaatcttgat gccgttcatc aacgaggccg tgtgggcgct
     5761 gcacgacggc gtcgcggaag ccgaggcgat cgacacgatc gccaggctcg ggttcgcgca
     5821 cccgctcggg ccgctggcgc ttgccgacct gatcggactc gacacctgca ccgcgatcat
     5881 ggaggtgctg cgggacggcc tcggggacga ccgctacgca ccgtgcccgc ttctgcgcga
     5941 gctcgtcgtt gccggaaaac tgggccggaa gagcggctca gggttctacg aatattcgta
     6001 gaaacgcata aacaacccta ggcgcagggt tttcgaccgt aaacgcgtct gtctctccga
     6061 gggctcaagt ctgccgagcc cgctgccgac aaaagcactc aggttccggg aaaagggagg
     6121 caccgcgcgt tgcggatcct gctggtagat gacgatcctg cgcttcgcac cttgctgaag
     6181 acgacgttcg aggtcgccga cgtagccgtg gtcgaagccg aagacgccgg tgccgcgcgc
     6241 cggaagatca agagcgcgag gccggacgtg atcgtgctcg acatcaacat gccggggacg
     6301 accggcctcg agctcagtgc cgagctgaag gccgcgccgg ccacgcgtga catcccgatc
     6361 gtgctcttga ccggctcgga gggcggcacc gaagtcgccg cgaagaaggc gggagccaac
     6421 gcgttcgtgc gcaagccgtt cagcccgctc gagttgctcg ccgtcgcgga gcgcctggcg
     6481 ggaggcggtc tgttcggcgt tcccttccgg gccgcggaga agcgcactca agggcccgac
     6541 gaggagttgc tgctctacgc gcgagacctg aggcacatgt tgggcgtcga gcgcgcccaa
     6601 cgcgagctgt tgcagagcgc gtacctccaa acggtctcgg cgctcgcggg cgcgctggag
     6661 tcgaaagaca cgggcacccg cgcgcactct cagcgcgtgc agagctacgc gacggctctc
     6721 gcacaggccg tcggcgaggg agcacttgtg cgcgacgaga gcgcgccgta cgggttcctc
     6781 ctgcacgacg tcggcaagat cgggattccc gacgggatcc tccagaagcc cggcccgctc
     6841 agcccggcgg agcggcgcaa gatgcagacg cacaccgtcc tcggcgaggc aatgctgtcc
     6901 ggcgtcgtct ttctgaaggg cgaaggcctc aagatcgtgc gctctcacca cgaacgctgg
     6961 gacgggcgcg gctatccgga cggcctcgtc cacgacgaga tcccactcgg tgcgcgcatc
     7021 ttcgcggtcg ccgatgcgct cgacgccatg acgagtcatc ggccctatcg ccgcgccatg
     7081 agctgggacg ctgcgcatgc cgagatcctc gagcagcgaa agcgtcagtt cgatcccgac
     7141 gtggtcgacg ccttcgtcat ggtcgagccg gcgctgcgcg agatccgccg cgagctcgcc
     7201 gcggcgtagc ccggccgagc acgcgggcac agggcccgta gaggcgccac gtatcatcgc
     7261 ctgcgacgtg gacttcgacc tcacgcagga ccagcgagag atccaggcgc tcgcgcgtga
     7321 ctttgcgcgg gccgagatcg atccacatgc agcccactgg gatcgtgagc atcactttcc
     7381 gcgcgagctg ttcgggaagc ttgccgagtt gggcctgatg ggcgtgtgcg tgccggagga
     7441 gtacggcggc gccggtgcag acttcctctc ctacgtcctg gtgctcgagg agttgtcgcg
     7501 cgcagacgct ggcgtcggcg tgaccgtcgc ggttcacacg agcgccgcga cgctaccgat
     7561 tctggcgtac gggaccgacg agcagatgtc gcgtttcgtg ccaccgctcg cacgcggcga
     7621 gcagctcggc gccttcgcgc tcacagaggc ggaggccgga tcagacgcgg gcgcgctccg
     7681 gacgcgagcg gaagcggcaa ctgacggctg gacgatcacc ggcgcgaagc aatggatcac
     7741 gaacgggcgc cacgcgggga cgttcatctt gttcgcgcgc accgatcccg aggtcgcggg
     7801 ggctcgaggc gtgagcgcgt tcatcctcga tgcagaacac gtgcgcgtga cccgtgacga
     7861 ggagaagctc gggctcaact cctctgtgac gagcgacatc gtggtcgagg gcgcacaggt
     7921 cggtcgagac cggctgcttc acgagcagaa ccgtggtttt cgagtcgcga tgggaacgct
     7981 cgacggcggc cgcatcggca tcggtgcgca ggcactcggg atcgcgcagg ccggctacga
     8041 ggttgcgcgc gagtatgcgg ccgagcggcg tgccttcggg catcgcatcg ccgacttcca
     8101 ggcgatccag cacaagctcg caaacatgtc gatggagatc gacgccgcgc ggctgctcgt
     8161 gtaccgcgcg gcctggttga aggagcaggg ccggcggcac accgaggagg gtgcaaaggc
     8221 gaagctgttc gcgtcagaga tggcgaggcg gcaaaccgcg gaggcgatcc aaattctggg
     8281 cgggtacggc tacacaaagg agtttccggt ggagcggtac taccgcgatg cgaagatcac
     8341 cgagatctac gaaggcacca gtgagattca gcgtctcgtg atcgcgcgca gcatcctcgg
     8401 gacaacggaa cgatctctgg tggccggttg accgtgcgcc ttctcttgat gtacacgcgc
     8461 gtacaggtcc ccacccgggt gggggttgtc acccaccacc cactgcaatc gacgataccg
     8521 ctgtccgacg cacgcgtggg tgccgagttc gtgtcaattc gtaggaccgt cgcttgagcc
     8581 cgcgtcgccg cgacgaaccg gtgcggctca cgcgcatcta cacgcgggcg ggggacggcg
     8641 gagagacgtc gctcggcgac ggctcgcggg tgccgaagct cgactgtcgc atcggtgcgt
     8701 tcgggaccgt cgacgagttg aacgcggccc tcggtgtcgt cctgacggcc gcgggcctcc
     8761 aggagcatct gcgcgcaccg ctcagtcgga tccagaacga ccttttcgac gtcggcgccg
     8821 acctctccgt tccgttcggc gtcacggatc gactccgagt cgagcaggcc gcagtggatc
     8881 gactcgaaca gctttgcgac gagctcaacg ccgaactgcc gatgctcacg agcttcatcc
     8941 tgcccggcgg aaccgaggcg gccgcccgtc tgcacgtcgc gcggacgacg tgccggcgcg
     9001 cagagcgcga cgcgctggcg gcgaacgagg agaacgagat caacccgctc gtgctcgcct
     9061 acctcaacag gctgtccgat tttctgttca tcgcagcgcg ctccgcgaac gccgacgcgg
     9121 gccgcgacga gccgctctgg aaaccgggcg gctgacccac gggagcgcgg agcgaacctg
     9181 aaccctcgct cgagtagtgc aggtggccgg cgctctggtg cgacggtcag gccaccttca
     9241 caggcttcgc cggctttgcc aggagcctct ggatcaggcg gccgagggtc gaggcgggtg
     9301 tagagcgggg tagcgcgatc gccgcgccgg ccttcagcga cgccgccatc tgcttcgggc
     9361 ccgaggtgag agcgacgatc ttggtcgagg ggaacactgc gtgcaggtca cggatgaggg
     9421 agacaccgaa ggcgtcgaca tcgaccacga tcacacggac atccgtgcgc ctactggccg
     9481 ctgcgtcgaa ggtcggctcg acgccggagg cggtgatgag tttgggcttc tgggtcgtga
     9541 gggctcccct gatgccgccg gctgccggcc cggccaggag cacccgagca ggcacggcga
     9601 tcacgttatt cgcacccacg agccggatcg tgagcttgtg ggagatcgac tcgcggcccg
     9661 agcgtgcggt ccagcgcatg gtgtaggcac cggagcggcg cacctgatgc ggtaggcgca
     9721 acttcacgat cgagcggccc gccttgagcg agaagcgcca ggtgtacagc ttcacgtggc
     9781 gcgggctgaa gagctccgcg gtcacgccgg acgcgcgtgt cacggagatc cgcgccgcga
     9841 tcttcttctt gagcacggc
//
LOCUS       DASVEU010000170         7844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_894834,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000170 DASVEU010000000
VERSION     DASVEU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894834"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF225_06860"
     CDS             complement(<1..396)
                     /locus_tag="VF225_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145014.1"
                     /translation="MIPERLARPLGSSSFRRLALGKSISYLGDWLMVAVLVGWVYQST
                     SSVAQVALLMAIRLVPPIVGGGLAASLVDRLPRRRVLIWSEAASAATIVGALLGVMAG
                     SRAAVFAFVGLCGLVSMVSTVAGNALIPMV"
     gene            692..3502
                     /locus_tag="VF225_06865"
     CDS             692..3502
                     /locus_tag="VF225_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7145015.1"
                     /translation="MTSARQQFSLLGPLTVSSDGTQIALGGQKRRALLAVLLLEANSV
                     VSRDRLIDALWGENPPETARNTIQVYISQLRKFLPEGALETAPPGYRLVIEADSVDLF
                     EFMRLSEEGRTALDAGDAAGGANTLRAALALWRGAALADLAWEPFAQAEVVRLEELRL
                     AALEDRIDADLALGRHGQLVGELEQLVGEHPLRERFRAQLMLALYRSGRQADALAVYQ
                     RARRALVDELGIEPSESLKQLERAILAHDPSLSPNVPQSAPPSPRQVPTPPTPLLGRE
                     QELAVLADVVRDMDARLVTLTGIGGIGKTRLALELIRRLAPEFQHGTAVVTLATLREP
                     ALVARTILEALAIREVGADPEEVLIEELKGTELLLLVDNFEQVLPAAGSIARLLDGSP
                     GLKVIVTSRAPLRIAAEREFPVPPLAEDEAAELFIARAQAADPSFALSERNAGAVAEI
                     CARLEGLPLAIELAAARTKLLPPMALLSRLGNRLELLTGGRRDAPQHQQTLRMTLDWS
                     YDLLEPEAQRLFERLGVFAGGCTLASAEAVCGGDGSVLEGLAALVDESLVRQRETDAG
                     EARFSMLAIVREYALERLSRSGDGDSTRRRHLEHFVSLAEEAEPKLADREQIAWFDRL
                     EDEHDNLRAALAFALDSDDPSSALRLAVGVRRFWQIHGYLVEGREALESALAATPSVP
                     SELRANALNMSGILAGEQGEFDAARVSFKAALEDAHAAGATRVISSALVNLGNLAFFS
                     GELDAARDLYKESIEYFASLDDLRGQALAKENVGLMALTADDVPEAVTWLTDALELAR
                     DVGDDREIGAASRSLAAAMVELGDSERATSLLAESLALARELAEPHGIAVCLDTFAGL
                     AATAGEVDRAATLFGASDAARASIGAKRQPDHQILYDRWLARTLAHLDTNTYATHYEN
                     GRTLTLDEACALALGQDAALIS"
     gene            complement(3516..3953)
                     /locus_tag="VF225_06870"
     CDS             complement(3516..3953)
                     /locus_tag="VF225_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145016.1"
                     /translation="MRFATKSCFVTLTVLLALLSIAWTAAAAAPAVPREKKIASYCSS
                     SGDVCYGIFNRRGKVYLRITTAARYFGRYKLCVRLLPPGSGAEHALRCGSFPVFRQGG
                     STWGSSVNYARQYPVTVPGRYRVTWKLGASPLGPPLQFRLPLA"
     gene            4049..4357
                     /locus_tag="VF225_06875"
     CDS             4049..4357
                     /locus_tag="VF225_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145017.1"
                     /translation="MYVSLTRVNTGDEPIENATIVGEEMVRWLHDIDGFLGFLLLSRE
                     ETTLGLSFWKTREVAERHRVARMEFLERMASVANVQVEEVLDYEVTFADLKGIVDFTG
                     "
     gene            complement(4354..4719)
                     /locus_tag="VF225_06880"
     CDS             complement(4354..4719)
                     /locus_tag="VF225_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7145018.1"
                     /translation="MSTDVEKETGEIDFEREVEDAVASLPRELRDVISNVAIVVEVEP
                     PPGQPLLGLYQGIPLTRRGSGYSGVLPDKITIYRGPLERLYGADRESLRRQIRRVVLH
                     EVAHHFGISDERLEELGRY"
     gene            complement(4773..5030)
                     /locus_tag="VF225_06885"
     CDS             complement(4773..5030)
                     /locus_tag="VF225_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145019.1"
                     /translation="MARDSTRLNVTLDAQYAEKLSRLAKRTHTQEGTLARSLLSQALD
                     EADPDPRHAADLLDGIPGAYERALLGRDQAKAGQTLPLDDL"
     gene            complement(5068..5199)
                     /locus_tag="VF225_06890"
     CDS             complement(5068..5199)
                     /locus_tag="VF225_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145020.1"
                     /translation="MSSNACSELRIAFSLSNAERRPDARSGGALVTGIALADETRPC"
     gene            complement(5453..5881)
                     /locus_tag="VF225_06895"
     CDS             complement(5453..5881)
                     /locus_tag="VF225_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7145021.1"
                     /translation="MRARVLVVCLLGLLCLPALALAAVSAGTKTFTTTLLGSNEVPKG
                     APKGSGKAVVTLNAGTGKVCWTFSSLKGISKVNASHIHKAAKGTSGPVVVAFFTGALK
                     TKGCVKASKSLISAIEKKPSAYYVNIHTAKYPAGAIRGQL"
     gene            complement(5964..>7844)
                     /locus_tag="VF225_06900"
     CDS             complement(5964..>7844)
                     /locus_tag="VF225_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145022.1"
                     /translation="VMSSASLPTFLLARAVTQSRRLPYLVAALSVCIPWIPLSSMLMT
                     EVVAYPAFVWAVLAVYRAVLSPRARNDVVMVVALGVATLARTQFLVLFLVVPIAFYVH
                     ELAFTEAPSRLSRARVAGLKLVESHRPLTAGYAFLLVAAVVLLAVGRLSSALGTYSVT
                     AEGNVLPSGMGRSLLEHLAPLGLGLGILPFVLGVAWLFANFVRPRAREQHAFAAIAVV
                     TFAVLLVEVTSYDLRFGQGRLHDRYLFYVVPLVLIAFAAALRAERRPHWSLFISGTLL
                     AVAFSRVPVVRYEKFNVDSPVAVLNDALLDAGGTVDGARVLLGLATIVLVLLYFFGAA
                     FLRRAQLTIVVLVFAVGAVTTETALAFNRLLTVDGTSGRPITLEQGGVFDWIDRKVGP
                     GAKVTMVPYPILYGDYWENVAYWWNVEFWNESVQRAATYEQAFTGTPETFPTTELTFD
                     RTTGRANVSPSVYAVQGIAEARFRLAGSELGEDRGVALIAVDRPWRAAWLTFDLYRDG
                     WTVPKFVGKIRVFAAPGQTQSLMRFLTISVRGPNDVPPRPFRIGSNATNWTAEAGEQP
                     TSKQISVCVPARGFADVQIHAPHYSPIYGDPRSEASFVSYARSGGVLITGIALADETT
                     PC"
BASE COUNT         1416 a         2596 c         2626 g         1206 t
ORIGIN      
        1 gaccatcgga atcagtgcgt tccccgcgac ggtggaaacc atcgagacga ggccgcagag
       61 gccgacgaac gcgaagacgg ccgccctgga gccggccatc acccccaaga gcgcaccgac
      121 gatcgtcgct gctgaggcgg cctcgctcca gatgaggacg cgtcgccggg gcagccgatc
      181 gacgagtgac gccgcgaggc cgcctccgac tatgggcggc acgagcctga ttgccatcag
      241 caaggcgacc tgcgcgaccg acgaggtcga ctggtagacc caaccgacga gcaccgccac
      301 catcagccaa tcgccgaggt aggagatgct cttaccgagc gcgagccggc ggaacgagga
      361 cgacccgagc ggccttgcga ggcgctcggg gatcaacctc gatgaggctt tcctcaggcc
      421 agccatctga tccgcggagc ggcgggcggc cccgcagcgc cgcccctccg gtccgacggc
      481 gcccctagct ccaactgttg ctgcgcctca cggttcctcc tctcgggcca cgggacggcc
      541 gcgacggcct cggccgtccc gcccctgatc gccgcagttc tctccggact gttggcttgc
      601 agtggattca cggccacgat cctggctccg gccgctgaag aaacgctgaa agagcgctga
      661 aatcgtgcta agacccacgc gggaaggagt ggtgacatcg gcccgtcaac agttcagcct
      721 cctcggcccc ctcactgtca gcagcgacgg aacacagatc gcgctcgggg ggcagaagcg
      781 acgcgcgctg ctggccgtcc tgctcctcga ggcgaatagc gtcgtctcgc gcgatcgcct
      841 gatcgacgcg ctctggggcg agaacccgcc cgagacggcg cggaacacga tccaggttta
      901 catctcgcag ctgagaaagt ttctgcccga aggcgcgctc gagaccgcac cgcccggtta
      961 ccggctggtc atcgaagccg acagcgtcga cctcttcgag ttcatgcggc tcagcgagga
     1021 aggaaggaca gcgctcgacg ccggtgacgc ggcgggtggc gccaatacct tgcgtgcggc
     1081 gctcgcactc tggcgcgggg cggcgctcgc cgacctcgcc tgggagccgt tcgcgcaggc
     1141 cgaggtcgtc cggctcgagg agcttcgact ggccgcgctc gaagaccgga tcgacgccga
     1201 tctcgcgctc ggccgccacg gccagctcgt cggcgagctc gagcaactgg taggcgagca
     1261 cccgctcagg gagcggtttc gcgcgcagct tatgctcgcg ctctatcgct cgggccggca
     1321 agccgacgcg ctcgccgtct accagcgcgc gcgcagggcc ttggtcgacg agctcgggat
     1381 cgagccgagc gaatcactca agcagctcga gcgagcaatc ctcgcccacg acccgtcgct
     1441 ctcgccgaac gtgccacaat cggccccgcc gtcgccgcgg caggtgccga cgcctccgac
     1501 tccgctcctc ggccgcgagc aggagctcgc cgtcctcgcc gatgtcgtca gggacatgga
     1561 cgcacggctg gtcacgctca ccgggatcgg cggcatcggc aagacccggc tggccctcga
     1621 gctcatccgg cgcctcgccc ccgagtttca gcacgggacg gccgtggtga ctcttgcgac
     1681 tctccgcgag ccggcgctgg tcgcgcggac gatcctggag gcactcgcga ttcgtgaagt
     1741 gggtgcggat ccggaggaag tgctgatcga ggagctgaag ggaactgaac tgcttctcct
     1801 cgtcgacaac ttcgaacagg tgctgcctgc ggcggggagc atcgcgcgcc tactcgacgg
     1861 ctcgccgggg ctgaaggtga tcgtgacgag ccgcgcgccc ctccgcattg cagcggaacg
     1921 cgagttcccg gtcccgcccc tcgccgagga cgaggcggcc gagctcttca tcgcccgcgc
     1981 acaggctgcc gatccgagtt tcgcgctgag cgagcggaac gcaggcgcgg tggccgagat
     2041 ctgcgcacgc ctggagggct tgcccctggc gatcgaactc gccgcggcgc gaacgaagct
     2101 gctaccgccg atggcgctgc tctcgcggct gggaaacagg ctcgagctcc tcaccggcgg
     2161 acggcgcgac gcgcctcagc accagcagac gcttcgcatg acactcgatt ggagctatga
     2221 cctgctcgag cctgaagcgc agcgcctctt cgagcggctc ggcgtgttcg caggcggctg
     2281 cacgcttgcg tcggccgaag ccgtctgcgg cggcgacggg tcggtgctcg aaggacttgc
     2341 agcgctcgtc gacgagagcc tcgtgcgcca gcgagagacc gatgcgggcg aggctcgctt
     2401 ttcgatgctc gcaatcgtgc gcgaatacgc gctcgagaga ctgtcacgct cgggcgacgg
     2461 cgactcgact cgccgccgac atctcgaaca cttcgtctcc ctggcggagg aggccgagcc
     2521 gaagctcgcc gaccgcgagc agatcgcctg gttcgaccgc ctcgaagacg agcacgacaa
     2581 cctgcgtgcg gccctagcct tcgcgctcga cagcgatgac ccgtcgtcgg cgctgcgact
     2641 cgctgtcggg gtacgcaggt tctggcagat tcacggctac ctggtcgagg gccgtgaggc
     2701 cctggagtcc gcgctcgccg ccacgcccag tgtgccgtcg gagctgcgtg cgaacgcgct
     2761 caacatgtcc gggatcctgg ccggagagca gggggagttc gacgcagccc gcgtgagctt
     2821 caaggccgcg ctcgaagacg cccacgccgc cggagcgacg cgcgtcatct catctgcgct
     2881 cgtcaacctc ggcaacctcg ccttcttcag cggtgagctt gatgcagcgc gcgatctgta
     2941 caaagagagc atcgagtact tcgcgtcgct cgatgacctc cgcgggcaag ctctggccaa
     3001 ggagaacgtc gggctcatgg ccctgacagc agatgatgtg ccggaggcgg tgacctggct
     3061 gacagacgcg ctcgagctgg cgcgcgacgt cggcgacgac cgcgagatcg gcgccgcctc
     3121 tcgctcgctc gccgcggcga tggtcgagct cggggacagt gagcgggcga cgagcctgct
     3181 cgccgagagc ctcgccctcg cgcgcgagct cgccgagccg cacggcatag ccgtctgcct
     3241 cgatacgttc gccggtctcg cggcgaccgc cggcgaggtg gaccgcgccg caacactgtt
     3301 cggcgcgagc gacgcggccc gtgcctcgat cggtgcgaaa cgtcagcccg accaccagat
     3361 tctctatgac cgttggctcg cccggacgct cgcgcatctg gacacgaaca cttacgcgac
     3421 tcattacgaa aatggacgaa cgttgacgct cgacgaggca tgcgccctgg cgctcggcca
     3481 ggatgcagcg ctgatttcct gagctggaac ggttcctagg cgagcggcag ccggaactgc
     3541 agtggcggac ccagcgggga cgcgcccagc ttccaggtca cgcggtacct gccgggaacg
     3601 gtcaccgggt actgacgcgc gtagttcacg gacgaccccc acgtcgagcc gccctgccgg
     3661 aagaccggga aggaaccgca gcgcagcgcg tgttcggcgc ccgagcccgg cgggagcaga
     3721 cggacacaga gcttgtagcg cccgaagtag cgcgccgccg tcgtgatccg caggtagacc
     3781 ttgcctctgc gattgaagat tccgtagcag acgtcgccgg atgagctgca gtagctcgcg
     3841 attttcttct cgcggggaac cgcgggagcc gctgccgctg cggtccaggc aatcgagagc
     3901 aaggcgagca gcacagtcaa cgtgacgaag caagacttcg tggcgaagcg cattgcccgg
     3961 cgaggctact gattgcatcg gccggcgaca acggcagcag cggacgtgcc aagattgcgg
     4021 acggaagcgt gctcgggaag ggagcagcgt gtacgtcagt ctcacgcgcg tcaacaccgg
     4081 ggatgagccg atcgagaatg cgacgatcgt gggcgaagag atggtcaggt ggcttcacga
     4141 catcgacggg tttctgggat ttctgctgct ctccagggaa gagacgacgc tcggactgag
     4201 cttctggaag acccgggaag tggctgagcg ccaccgcgtc gctcgaatgg agttcctcga
     4261 gcggatggcg tcggtcgcca acgtgcaggt ggaagaagtg ctcgactacg aagtcacgtt
     4321 cgcggatctc aaagggatcg tggacttcac cggctagtac cgcccgagct cctcgagacg
     4381 ctcgtcgctg atcccgaagt ggtgcgcaac ttcgtggagg accacgcgcc ggatctgccg
     4441 gcgcagactt tcccggtcgg cgccgtagag gcgctcgagc ggcccgcggt agatcgtgat
     4501 cttgtccggc agaacgccgc tgtaaccgct tccgcgtcgc gtcagcggaa tgccctgata
     4561 caggccgagt agcggttggc cgggcggcgg ctccacctcg acgacgatcg cgacattgga
     4621 gatgacatcc cgaagctcgc gagggagcga ggccactgca tcctcgacct cccgttcgaa
     4681 gtcgatctca ccggtctcct tctcgacatc tgtggacatg ggtcgagcgt agcgggagtc
     4741 ggcacatccg acgcacgaac ctgccgcgat cgctagagat cgtcaagcgg gagtgtctgg
     4801 ccagccttcg cttggtcgcg cccaagcagc gcgcgttcgt aggcgccggg gatgccgtcg
     4861 aggagatccg cagcgtggcg tggatcagga tcggcttcgt cgagggcctg cgaaaggagg
     4921 gaccgcgcca gggtcccctc ctgcgtatgt gtccgcttgg cgagccggga gagcttctcg
     4981 gcgtactgag cgtcgagcgt gacattcagc cgagtgctgt cacgggccac gagcagagaa
     5041 taggacgagc aagcacgaaa agtggaacta gcagggcctg gtctcgtccg cgagcgcgat
     5101 gcccgtcacg agcgcgcctc ccgagcgggc atcgggccgg cgctcggcat tcgacagcga
     5161 gaaggcgatt cgcaactccg agcaagcgtt cgaagacatg gcaagtcgct gctctctgaa
     5221 acgtgcgttg aaggttcgtc acagtgtctg acactgggac ctggctacga gcgacgagag
     5281 gccaaacggc ttgactacac cgatcgcgcg gtatgcggga cttcctcgtg ggcagtacgg
     5341 caaagtgtct gacaccaaga cgtcgctata gggggcacct ttcatagggg acgccctcgc
     5401 ttccgcgagg ccgtcgttcg gcgacctcgc ggaagcggga gtgttgtgct acctacagct
     5461 gtccgcggat tgctccagcc ggatacttcg ccgtgtggat gttgacgtag taggcactgg
     5521 gcttcttctc gatagcactg atcagggact tcgatgcctt cacgcagcct ttcgtcttca
     5581 acgcgccggt gaagaaggcg acgacaacgg ggccagacgt tcccttcgcc gctttgtgga
     5641 tatgggaggc gttcactttg ctgattccct tcaggctcga gaatgtccag cagaccttgc
     5701 ccgtcccggc attgagtgtg accaccgcct tgccggatcc cttcggggcg cctttcggca
     5761 cctcattgct gccgagcaac gttgtcgtga acgtcttggt ccccgctgaa acggccgcta
     5821 gtgcaagcgc tggaaggcac agcaggccaa gcaggcagac gacgagtaca cgagcacgca
     5881 tacgtcctcc gatcggttgc aggtagccgc ttctactccg attgcctgca ggcggtttgc
     5941 cactcgctct agcggcccac gagctagcaa ggcgtcgtct cgtcggccag cgcgatgccg
     6001 gtgatgagca cgcctcccga gcgcgcatag ctgacgaagc tcgcctcgct cctcgggtcg
     6061 ccgtagatcg gtgagtaatg cggtgcgtgg atctgcacat ccgcgaaccc gcgcgccggc
     6121 acgcagacgg agatctgttt gctcgtcggc tgctcgccgg cctctgccgt ccaattggtc
     6181 gcgttcgagc cgatgcgaaa cggccgcggc ggaacgtcgt tcggaccgcg aaccgagatg
     6241 gtgaggaacc tcatcagcga ctgcgtctgt cccggtgccg cgaagaccct gatcttgccc
     6301 acgaacttgg gcacggtcca accgtctcgg tagagatcga acgtcagcca cgcggcgcgc
     6361 cagggcctgt cgactgcgat caacgccacg cctcggtcct cccccagttc ggagccggca
     6421 agccgaaatc tcgcctccgc gatgccctgc acggcgtaca cgcttggcga gacgtttgcg
     6481 cgtccggtgg tgcggtcgaa ggtcagctcc gtcgtcggaa acgtctccgg cgtcccggtg
     6541 aacgcctgct cgtacgtggc ggcacgctgc accgattcgt tccagaactc gacgttccac
     6601 cagtaggcga cgttttccca gtaatcgccg tagaggatcg gatagggaac catcgtcacc
     6661 ttcgcccccg gtccgacctt gcggtcgatc cagtcgaaca ctccaccctg ctcgagcgtg
     6721 atcggccgcc cgctcgtacc gtccacggtc aggagtctgt tgaaggcaag agcggtctcg
     6781 gtcgtgaccg caccgacggc gaagacgagc acgacgatcg tcagctgcgc gcggcgaagg
     6841 aacgctgcgc caaagaagta aagaagcacg agcacgatcg tcgcgagccc gaggaggact
     6901 cgggcaccgt cgaccgtgcc gcccgcgtcg aggagcgcgt cgttgaggac cgcgaccggc
     6961 gagtcgacgt tgaacttttc gtaacgcacc accgggacgc gtgagaaggc gacagccagc
     7021 agcgtccccg agatgaagag cgaccagtgc gggcggcgct ccgcacggag cgccgccgca
     7081 aaggcaatca ggacgagcgg cacgacgtag aacagatagc ggtcgtgcag ccttccctgg
     7141 ccgaagcgaa ggtcatacga ggtcacctcg acgagcagca ccgcgaatgt gacgacggcg
     7201 atcgctgcaa aggcgtgttg ctcccgcgca cgcggccgga cgaagttcgc gaagagccag
     7261 gcaaccccca gcacgaacgg caggatcccg aggccgaggc cgagcggcgc gaggtgctcg
     7321 agtagtgagc ggcccatgcc actcggaaga acgtttccct cggccgtcac cgaatacgtg
     7381 ccgagtgcgc tcgacagtcg gccgacggcg agcagcacga ctgcggcgac caacaaaaag
     7441 gcatatccgg ccgtcaacgg ccgatgcgac tccacgagct tcagcccggc gacacgggcg
     7501 cgcgacagac gagaaggcgc ctccgtgaat gccagttcgt gaacgtagaa cgcaatggga
     7561 acgacgagga agaggacgag gaactgcgtt cgcgcgagag tcgccacccc gagcgcgacg
     7621 accatcacga cgtcgttccg cgcccgcggc gagaggacgg cacggtagac ggccagcacc
     7681 gcccacacga acgcgggata cgccaccacc tcagtcatga gcatggacga gagcgggatc
     7741 cagggaatgc aaacgctcaa tgctgcgacg agatagggca gccgccgact ctgcgtgaca
     7801 gcccgtgcga gcaggaacgt cggcaggctc gcagagctca tgac
//
LOCUS       DASVEU010000171         8510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_314776,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000171 DASVEU010000000
VERSION     DASVEU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314776"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..687)
                     /locus_tag="VF225_06905"
     CDS             complement(61..687)
                     /locus_tag="VF225_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7145023.1"
                     /translation="MGLEQLSERYAEHNRVERGAGFVFAGPERVELFRRYVGGPGRHV
                     LDLGCRDGALTQAYAGGNEIVGVDADRDALAEAEKLGIETRWADLDDALPFEDGSFDV
                     VVAGELLEHLRDPRRVVSEVLRVLRPRGTFVASVPNAYRLKNRLRFLLGRKPEDDPTH
                     LQMFTPHDLRALLAGFEDVQLHLIAGRLVPLHARLFANDIVFSGRKSR"
     gene            742..2238
                     /locus_tag="VF225_06910"
     CDS             742..2238
                     /locus_tag="VF225_06910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7145024.1"
                     /translation="MSTKPAAPAEPRPAPVEDIRSARPYLLSGTTLLAVARRLASVAT
                     LVVLDLLGLVLGIFVALIFRELYLGQWPPLWGILWDSETEWLPFLTVITVLVFWVGGL
                     YRRRELRTGVGQIVLSIVLVAVITLAFGLGTGYHFTTYGLTPTAVVTTALFIGILRAS
                     YEVLTRDVLTLTGVRRRTVLVGEGEQLAALRRTLGADRSGIRYDFLGAIAPGGDAGDL
                     RVLGGLDAVPRILREHDVHELIVTDGGFAERELLELVEEAHRYGAKVKIAPRTTELLL
                     QRAEYIPGEGAPLFELRPPALAGSEWALKRGFDLVVSSIVIVAGLPLWLAVAGAVKLS
                     SRGPVFYRDRRIGLSERPFGMMKFRTMYADAAARQGALEADNEASGPLFKIKDDPRVT
                     PVGRFLRRYSLDEMPQVLNVLWGEMSLVGPRPLPIRDYEQLLPWHRKRYLVLPGMTGL
                     WQVSGRIDLSFDDLVRLDFYYIENWSVWLDITILARTLPAVLARRGAY"
     gene            2269..3483
                     /locus_tag="VF225_06915"
     CDS             2269..3483
                     /locus_tag="VF225_06915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7145025.1"
                     /translation="MAGAKILAVASATDLDFRYGCTPAWWQIWKGLNEVGVDLIVTPY
                     RGRAIESPWWRTAPNPLYREAELFAGARGLAARLRGDTLLRRGEDNPDDTVGDRLTRE
                     AIWHYVTPRWQRHLERILERERDVAAVIVFTVPMAHLRGIPTRLRERFDVPVVFYDGD
                     VPMSLPEFGGMDTGFNYYHGADPSEYDLVLSNSEGGLDRLRELGAQRAEAVFWAADPE
                     LFQPAAVEKEHDVFFYGYGDKFRREWMQDLVGEPSRHDPDIDFALGGRDFQGDTGNAR
                     LIGDVPFNVFAQAISRARINLNITRRSHATVYASSTCRPFELAASGAAIVSNPHEGIE
                     RWFDVGSELRVVSNADQATAAYRELLDDPGAAETMGQRARERVLDEHTYAHRARQILE
                     LVGLQSPVAAGG"
     gene            3476..4261
                     /locus_tag="VF225_06920"
     CDS             3476..4261
                     /locus_tag="VF225_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7145026.1"
                     /translation="MAELAEASARPREDVLAQIAELRRIAIVPALNEEETIGRVIDEI
                     RAFDPGFDIVVVDDGSTDRTAGLAADRGVHVLRLPFNLGIGGAVQTGYRFAFERGYDI
                     AVQVDGDGQHDPSQLPTILAPVVAGEADLCVGSRFASGSGYQSSFARRFGIRLFAWVV
                     SAVVRQKVTDTTSGFRAVNRKGIALFAADYPHDYPEVEATIMCVKHRLRLQEVPVEMR
                     ERGGGASSITALRSIYYMTKVLLAIFVGLFRRYAVPLEKEHRR"
     gene            4258..4623
                     /locus_tag="VF225_06925"
     CDS             4258..4623
                     /locus_tag="VF225_06925"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX7145027.1"
                     /translation="MTPLPVSIAAAVASFLLLVVVFELIRSRRLRERYALLWLLTGAV
                     LLALSVWRDGLNTIAGWFGVETYPPAILGAVGALFILMVLLHYSTVISRLSDQNTILA
                     QRLALLEQQQREGVTESPR"
     gene            complement(4604..6283)
                     /locus_tag="VF225_06930"
     CDS             complement(4604..6283)
                     /locus_tag="VF225_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145028.1"
                     /translation="MSSEAAPAPPAVAAIGARLAKASAILSSVRPAYLLGVLVVVQWL
                     AVLALAATVRHNGWLYYAGGDQLWHYSGAYLLAHGHLPPAYVGYGWSIMLLPVSWFAG
                     ASLVSALPAIVVFNTVILLPVALLCVYGIASRIAGRLFGYFAAVLWIALPYLGILFVE
                     PGYHQKYTELTLPQALGLSSVPDFPATVALLVSAFFCLRTLDSAGWQPAAAAGLAAGW
                     SIAIKPSNAIFLVAPALLLIVERRRALPVFVAGLLPAALTLALWKFRGLGELAAAPAE
                     PVQLAAGVGDLIHRIHSPSLNSWAHLRQVLDALREHFWAARVMEWLPVAGSIGLLARS
                     RRAFLLVGSWFIVFLLAKGTYIPASIDDASFFRILMPAFPAYLLLAAAIVLLVPGVRA
                     RPARPAPILSGRRLSIVFAAAVLVFAALPLGVIGATPRLHDGGRQAVRLGDNLVPVVP
                     AVGLAATTAGGVVHLEWRPRPARGTAVFYRVLRAEGAGGGAQCAGRLRNSSDNCELSA
                     ADLGSVRAPSFDDRPGPGSWSYRIGVAANWLNDPSLGDVYFVSAPVSVAIP"
     gene            complement(6280..7206)
                     /locus_tag="VF225_06935"
     CDS             complement(6280..7206)
                     /locus_tag="VF225_06935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX7145029.1"
                     /translation="MKILVTGGGGFLGSHLVERLEADGHDVYVARRADYDLTKWDDTA
                     RVFEDAQPERVFHLAGEVGGIGANRVNPGRFWYANLMMGAHVLELSRKHGLGKLVIAG
                     TVCAYPKFAPVPFREDDLWDGYPEETNAPYGVAKKAILVGAQSYREQYGLDSIFLLPA
                     NLYGPRDNFDLETSHVIPGLIRKMLDNPERVTLWGDGSPTREFLYVDDCVEGLVLAAD
                     RYDGGEPVNLGTGVEISIRELAEQIADLTGFAGEIEWDSSMPNGQPRRSLDASRAHEL
                     FGFEAHTPLRAGLERTIAWYREHAGATAGAQA"
     gene            complement(7203..8345)
                     /locus_tag="VF225_06940"
     CDS             complement(7203..8345)
                     /locus_tag="VF225_06940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7145030.1"
                     /translation="MKADQHDSTWTDDAVADLRRDFSEFFSGRTCLVTGADGFMGSHL
                     TDALVQLDANVHAFVRATSSGALNNIAPLRERLKVHFADLTDKTSIDYLIRELLHVPD
                     KPYVFHLGAQAHVGESWHRPYETVTANTIGTLNLLQSIVDNGLELEKFDTAGTSEEYG
                     NVHEDVAHHHDFDEEGGLILHERSPINPKSIYATSKVAADFLTMNYHDAFGVPGVVTR
                     MFNNYGPRQNPRYVTGTIITQALTRETVELGQLEPMRDFCFCTDGVRGHLMVAARGIP
                     GDLYVYGQGKNVSMREWAELILRVGREQGYWEAREIVSTAERYRPGASEVMALRVGYD
                     KLNRETGWDPHVSWDDGVARTIAWFAENRPRWIGRVDWLSQTRARA"
BASE COUNT         1385 a         2814 c         2899 g         1412 t
ORIGIN      
        1 tttgcgcttc ggcctcggct tcctcgcact gaagaggacc gcgacggacc tcgacgagct
       61 tcagcgcgac ttgcggcccg agaagacgat gtcgttcgca aacagccgtg catgaagcgg
      121 gacgaggcgg cccgcgatga gatgcagctg aacgtcttcg aagccggcga gcagcgcgcg
      181 caggtcgtgc ggcgtgaaca tctgcagatg cgtggggtcg tcctcgggtt tgcgcccaag
      241 taggaagcga agcctgttct tcaagcggta ggcgttcgga acggacgcaa cgaacgtgcc
      301 acgcggtcgc aggacacgta gcacctcgga gacgacccgt cgcggatcgc ggaggtgctc
      361 gagcagctcg cccgcgacga cgacgtcgaa ggagccgtcc tcgaacggca gcgcgtcgtc
      421 gagatccgcc caacgggtct cgatcccgag cttctcggcc tcggcgagag cgtcgcggtc
      481 ggcgtcgacg ccgacgatct cgttcccgcc ggcgtaggcc tgcgtcagcg ccccgtcacg
      541 gcagccgaga tcgaggacgt ggcggccagg cccaccgaca tagcgccgaa aaagctcgac
      601 gcgctcggga cctgcaaaga cgaagccggc gccccgttcg acgcggttgt gctcggcgta
      661 ccgctcgctc agctgctcca ggcccaccgg tcgagcctag cgttgtcggc cactggaacc
      721 cccggaagta cactccggcg ggtgagcacg aagccagccg cgccggccga gccccggccg
      781 gcgccggtcg aagacatccg ctcggcccgg ccctacctcc tctccggcac cactctcctg
      841 gccgtcgccc ggcgcctcgc cagcgtcgcg accctcgtgg tgctcgacct gttgggcctc
      901 gtcctcggca tctttgtcgc gctcatcttc cgggagctct acctcggcca gtggccgccg
      961 ctctggggaa tcctctggga cagtgagacc gagtggttgc ccttcctcac ggtgatcacg
     1021 gtgctcgtgt tctgggtggg cggcctctac cggcggcgtg agctccgcac cggcgtcggc
     1081 cagatcgtgc tctcgatcgt gctcgtcgcg gtgatcacgc tcgcattcgg cctcgggacg
     1141 ggctaccact tcacgaccta cggcttgacg ccgacggctg tcgtcacgac ggcactcttc
     1201 atcggcatcc tccgcgcaag ctacgaggtg ctgacacgtg atgttctgac tctcaccggc
     1261 gtacgacgtc ggacggtgct cgtcggagag ggagagcagc tggcggctct gcgccgcacg
     1321 ctcggcgcag atcgcagcgg gattcgctac gacttcctcg gcgcgatcgc tcctggtggc
     1381 gatgccgggg acctgcgcgt gctgggtggc ctcgacgcgg tgccacgcat actgcgcgag
     1441 cacgacgtcc acgagctcat cgtgacggat ggtgggtttg ccgagcgcga gctgctcgag
     1501 ctcgtcgaag aggctcaccg ctacggagcc aaggtcaaga tcgcgccgcg caccaccgag
     1561 ctgctcctgc agcgggccga atacattccg ggcgaagggg cgccgctgtt cgagttacgc
     1621 cctccggcgc tcgccggatc cgaatgggca ttgaagcgcg gcttcgacct cgtggtgtcg
     1681 agcatcgtca tcgtcgccgg cttgccgctc tggctagcgg tggcaggtgc ggtcaagctc
     1741 agctcgcgcg ggccggtgtt ctatcgcgac cggcgtatcg gactcagcga gcggcccttc
     1801 gggatgatga agttccggac gatgtacgcc gacgcggccg cgcggcaggg cgcgctcgag
     1861 gccgacaacg aggcctccgg tccgctcttc aagatcaagg acgaccctcg ggtcacgccg
     1921 gtcgggcggt tcctgcgccg ctactcgctc gacgagatgc cgcaggtcct caacgtcctg
     1981 tggggcgaga tgtcgctcgt cggtccgcgg ccgcttccca ttcgcgacta tgaacagctg
     2041 ctgccctggc accggaagcg ctatctcgtg ctcccgggga tgacgggcct ctggcaggtc
     2101 tccggccgca tcgatctgag cttcgacgat ctcgtccggc tcgacttcta ctacatcgag
     2161 aactggtcgg tctggctaga catcacgatc ctggcaagga cgctgcccgc cgtcctggcg
     2221 cggcgcggcg cttactgagc cgacacatcc gttcgccata ctgatcgcgt ggctggtgcc
     2281 aaaatcctcg cggtcgcgtc cgcgaccgac ctcgacttcc gctacggctg tacgcccgcg
     2341 tggtggcaga tctggaaagg cctcaacgag gtcggggtcg acctcatcgt cacgccgtac
     2401 cgtgggcgcg cgatcgaatc gccctggtgg cggacggcgc ccaatccgct ctaccgggag
     2461 gccgagctct tcgcgggagc ccgcgggctc gccgcgcgcc tgagaggcga cacgctgctg
     2521 cggcgcggcg aggacaaccc ggacgacacg gtcggagaca gactgacgcg cgaggcgatc
     2581 tggcactacg tcacgccgcg ttggcagcgg catctcgagc ggatcctcga acgggaacgc
     2641 gacgttgccg cggtgatcgt cttcaccgtg ccgatggcac acctgcgcgg tatcccgacg
     2701 cgactgcgtg aacgattcga cgtcccggtc gtgttctacg acggcgacgt gcccatgagc
     2761 ctgccggagt tcggaggaat ggacacgggg ttcaactact accacggtgc cgatccgtcg
     2821 gagtacgacc tcgtgctctc gaactccgag ggtgggctcg accgcctgcg tgagttgggg
     2881 gcgcagcgcg cggaagccgt cttctgggct gcggacccgg aactgtttca gccggcggcc
     2941 gtcgagaagg agcacgacgt cttcttctat ggctacggag acaagtttcg gcgcgaatgg
     3001 atgcaggacc tggtcggcga accgagccgg cacgatccag acatcgactt cgccctcggc
     3061 gggagggact tccaggggga caccggaaac gcgcgcctga tcggcgacgt tcccttcaat
     3121 gtcttcgcac aggcgatctc gcgcgcgagg atcaacctga acatcacgcg gcgctctcac
     3181 gcaaccgtgt acgcgtcgtc gacctgccgg ccgttcgagc ttgccgcgtc cggggcggcg
     3241 atcgtgtcca atccacacga agggatcgag cggtggttcg acgtcggctc ggagctgcgc
     3301 gtcgtctcga acgcggacca agcaaccgct gcctatcgcg agctgctgga cgatcccggg
     3361 gcagccgaga cgatgggaca gcgcgcgcgg gaacgtgtgc tcgacgagca cacctacgcg
     3421 catcgtgctc ggcagatcct cgagctcgtg ggtctgcagt cgccggtggc cgccggtggc
     3481 tgagctcgcg gaggcctcgg cgcggccgcg cgaagacgtg cttgcgcaga ttgccgagtt
     3541 gcggcgcatc gcgatcgtcc ctgcgctgaa cgaggaggag acgatcggcc gagtcatcga
     3601 cgagattcgc gccttcgatc cgggattcga catcgtggtg gtcgacgacg gctccacgga
     3661 ccggacggcc gggctggccg cggatcgcgg cgtgcacgtg ctccggctcc ccttcaacct
     3721 cggcatcggc ggtgccgttc agacgggcta tcgcttcgcg tttgagcgcg gctatgacat
     3781 cgcggtccag gtcgacggcg atggacagca tgacccgtcg cagctgccaa cgatcctcgc
     3841 acccgtcgtc gccggggaag cggatctctg tgtgggatcg cgtttcgcga gcggcagcgg
     3901 ctatcagtcc tccttcgcgc gccggttcgg gatcaggctg ttcgcgtggg tggtgtcggc
     3961 cgtcgtgcgc caaaaggtga cggacaccac ctcaggtttc cgggccgtca acaggaaggg
     4021 catcgcgctc ttcgccgcgg actatccgca cgactatccc gaggtcgagg ccacgatcat
     4081 gtgcgtcaag cacaggctgc gcctgcagga ggttccagtc gagatgcgcg agcgaggggg
     4141 aggcgcatcg tcgatcactg cgctgcgctc gatctattac atgacgaagg tgctgctcgc
     4201 gatcttcgtc ggcctctttc gtcgctacgc cgtcccgcta gagaaggagc atcgccgatg
     4261 acaccgcttc ccgtatcgat cgccgcggcg gtcgcgtcct tcctgctgct cgtggtcgtc
     4321 ttcgagttga ttcgctcgcg gcgtctgcgc gaacgctatg cgctgctctg gctgctgacc
     4381 ggcgccgtgc tcctggcgct gtccgtctgg cgtgacggac tcaacacgat cgcaggttgg
     4441 ttcggcgtcg agacgtatcc gccggcgatc ctcggcgccg tgggcgcgct cttcatcctc
     4501 atggtgctgc tgcactactc gaccgtcatc tcacggctct ccgaccagaa cacgatcctt
     4561 gctcagcgcc tcgctctgct cgagcagcag cagcgcgaag gcgttacgga atcgccacgc
     4621 tgaccggagc gctgacgaag tagacgtcgc cgagtgacgg atcgttgagc cagttcgcgg
     4681 cgaccccgat gcggtaagac caacttccgg gaccaggtcg atcgtcgaac gacggcgccc
     4741 gcaccgatcc caagtcggcc gcggacagct cgcaattgtc ggacgagttg cgaagcctcc
     4801 cggcacactg cgcacctccg ccggcgccct ccgcccgcag cacccgatag aagactgcgg
     4861 tcccacgggc cggacgcggt cgccactcga gatgaacaac gcctccagcg gtcgtagccg
     4921 caagcccgac ggcgggcacg accgggacga ggttgtctcc caggcgaact gcttggcggc
     4981 cgccgtcgtg gagccgggga gttgcgccga tcacgccgag tggcagcgct gcgaatacca
     5041 agacggcggc ggcgaagacg atgctcagcc tgcggccgct cagaatcgga gctggtcgtg
     5101 ccggccgggc acggactccg ggtacgagga ggacgatcgc tgccgccagc aagagataag
     5161 cgggaaacgc cggcatcagg atgcggaaga agctcgcgtc gtcgatcgat gcgggaatgt
     5221 acgtcccctt ggcgagcagg aagacgatga accagcttcc gacgagcagg aaggcgcgcc
     5281 gggatctcgc gagaagtccg atggagcccg cgaccggaag ccattccatt acacgcgccg
     5341 cccagaagtg ctcgcgcaag gcgtcgagca cctggcgcag atgggcccag ctgttcaggc
     5401 tcgggctgtg aattcggtgg atcagatccc cgactccagc agcgagctgg accggctctg
     5461 cgggtgcggc cgcgagctcg cccaggccgc ggaacttcca gagcgcgagg gttagagccg
     5521 cgggcaagag gcccgcgacg aaaacgggca gcgcgcgccg ccgttcgacg atcagcagca
     5581 atgcgggcgc gaccaggaag atcgcgttcg agggcttgat cgcgatcgac cagccggcgg
     5641 caagtcctgc agctgcggcc ggttgccagc ccgccgagtc gagcgtgcga aggcagaaga
     5701 acgcggagac caggagtgcg acagttgcag ggaaatccgg cacagagctc agccctaggg
     5761 cttgaggcag tgtgagctcc gtgtacttct ggtggtagcc cggctccacg aacaggattc
     5821 cgaggtaagg aagcgcgatc cagagcaccg cagcgaagta gccgaagagt cggcccgcga
     5881 tccggctggc gatcccgtac acgcataaga gcgcgaccgg cagcaggatc acggtgttga
     5941 agacgacgat tgccggcagc gcggagacga ggctcgcgcc ggcgaaccac gaaacgggca
     6001 gcaacatgat cgaccagccg tagccgacat aggctggcgg caggtgcccg tgcgcgagca
     6061 ggtaggcacc ggagtagtgc cagagctggt cgccgcctgc atagtagagc caaccgttgt
     6121 gcctgaccgt cgcggcaagg gcgagcaccg ccagccactg cacgaccacg aggacgccga
     6181 gcagataggc ggggcgaacc gagctcagga tcgcggatgc cttggcgagg cgcgcgccga
     6241 tcgccgcgac cgctggtggc gccggcgccg cttccgagct cacgcctgcg cgccggcggt
     6301 cgcgccggcg tgctcgcggt accacgcgat cgtccgctcg agcccagcgc gcagcggtgt
     6361 gtgcgcctcg aagccgaaca gctcatgggc gcggctcgcg tcgagactcc ggcgaggctg
     6421 gccgttcggc atcgagctgt cccactcgat ctcgcctgcg aagccggtga ggtccgcgat
     6481 ctgttctgcg agctcgcgaa tcgagatctc gacgcccgtg ccgaggttga ccggctcgcc
     6541 gccgtcgtag cggtccgcgg cgagtaccag gccttcgacg cagtcgtcca cgtagaggaa
     6601 ctcgcgtgtc ggcgagccgt cgccccagag cgtgacgcgc tccgggttgt ccagcatctt
     6661 ccggatcagc cctggaatga cgtgtgatgt ctcgaggtcg aagttgtcgc gcggcccgta
     6721 gaggttcgcg ggcaggagga agatcgagtc gagcccgtac tgctctcggt aggactgtgc
     6781 gcctacgagg atcgccttct tcgcaacgcc gtacggggcg ttggtttcct cgggatagcc
     6841 gtcccagagg tcgtcttcgc ggaacggcac gggtgcgaac ttcggatagg cgcacacggt
     6901 gccggcgatc acgagcttgc cgaggccgtg cttgcgtgac agctcgagga cgtgcgcgcc
     6961 catcatcagg ttcgcgtacc agaaccgccc cggattcacg cggttcgccc cgatgccgcc
     7021 gacctcgccc gcgaggtgga acacacgctc gggctgggcg tcctcgaaca ctcgggcggt
     7081 gtcgtcccac ttcgtgaggt cgtagtctgc gcggcgcgcg acatagacgt cgtggccgtc
     7141 ggcctccagg cgttcgacga ggtgcgagcc gagaaagccg ccacctcctg tgacgaggat
     7201 cttcatgcgc gagcgcgcgt ttgcgacagc cagtcgaccc ggccgatcca gcgcggacgg
     7261 ttctccgcaa accaagcgat cgtccgtgcg actccgtcat cccacgagac gtggggatcc
     7321 cagccggtct cgcggttgag cttgtcgtag ccgacccgca gcgccatgac ctcgctggct
     7381 cccggacggt agcgctcggc ggtcgacacg atctcacgag cttcccagta gccctgctcg
     7441 cgccccacgc ggaggatgag ctcggcccac tcgcgcatcg acacgttctt tccctgcccg
     7501 tagacgtaga ggtcgccggg tatcccgcgc gccgcgacca tgaggtgccc gcggactcca
     7561 tcggtgcaga agcagaagtc ccgcatcggc tcgagctggc cgagctcgac ggtctcgcgc
     7621 gtgagcgcct gagtgatgat cgtcccggtg acgtaccgcg ggttctggcg cggtccgtag
     7681 ttgttgaaca tccgtgtgac gacgcccggc acgccgaacg cgtcgtggta gttcatggtg
     7741 aggaaatccg ccgcgacctt cgaggtcgca tagatcgact tcggattgat cggcgagcgc
     7801 tcgtggagga tcaaaccgcc ctcctcgtcg aagtcgtgat ggtgcgccac gtcctcgtgc
     7861 acgttcccgt actcctccga tgtgcctgcg gtgtcgaact tctcgagctc tagaccgttg
     7921 tcgacgatcg actggagcag gttcagcgtc ccgatggtgt tcgcggtcac cgtttcgtac
     7981 gggcggtgcc acgattcgcc gacgtgcgcc tgcgcgccga gatggaagac gtacggcttg
     8041 tcgggcacgt gcaacagctc cctgatcagg tagtcgatcg acgtcttgtc cgttagatcg
     8101 gcgaagtgca ccttgagccg ctcgcgcagc ggggcgatgt tgttcagggc gccgctggag
     8161 gttgcgcgga caaacgcgtg cacatttgcg tcgagctgca cgagcgcgtc ggtgaggtgc
     8221 gaacccatga acccatcggc gccggtgacc agacaggtcc tgccgctgaa gaactcgctg
     8281 aagtctcgcc gcaggtcagc gacggcatcg tccgtccagg tggagtcgtg ttggtctgct
     8341 ttcatgcagc ggtgcccgcc gacgagcagc ccaagggtag cccgggacgc ggccggagcg
     8401 ccgttcgaag cggcgccttt acaggtctct catatgtgat cttgagcctc gcggccgccg
     8461 tcgccggtgc ttttctcgcg cacaagttcg gacgaagccg ggagaccgac
//
LOCUS       DASVEU010000172         8293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_315067,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000172 DASVEU010000000
VERSION     DASVEU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315067"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF225_06945"
     CDS             <1..195
                     /locus_tag="VF225_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145031.1"
                     /translation="QHLNVLKEAGLVSERRNGTRRLYLARPEGLAELKDFLEEFWDVR
                     LETLKREAEKEERKKHGRNH"
     gene            179..646
                     /locus_tag="VF225_06950"
     CDS             179..646
                     /locus_tag="VF225_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7145032.1"
                     /translation="MDATTETISVERTISIAATPETVWEFFVDPEKLTRWKGMKAELD
                     PRRGGVYLCEVIPGHTARGEYVELDPPRRLVFTWGWDNEEGVPPGSSTIEVELTPEGE
                     GTSLHFVHRDLPSAESAESHAQGWDHYLPRLEIVATGGDPGEDPWLTQDMQAS"
     gene            666..1013
                     /locus_tag="VF225_06955"
     CDS             666..1013
                     /locus_tag="VF225_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145033.1"
                     /translation="MAHPVMHFEVTGKDLDKLQSFYGELFGWNTSKIPGEMPYAMVEK
                     EGDGIGGGIGQTQNGNGHVTFYVGTDDPQATLDKAERLGGKTIMPVTELPEVTIALFA
                     DPEGHVVGLAKGM"
     gene            complement(1021..2361)
                     /locus_tag="VF225_06960"
     CDS             complement(1021..2361)
                     /locus_tag="VF225_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7145034.1"
                     /translation="MSSLTELRELHRGPVIAPDDRGFDVARATFNGMIERHPELIVRP
                     LDVVDVVTAVRFARETDLPVAVRGGGHGVAGHCIGDGSLVVDLRLLREVVVDPESRTA
                     TCGGGALWEDLDTPAQRHGLATPGGTFGDTGIAGLTLGGGIGHLTPSYGLTLDNLLAA
                     TVVTADGSVLTASESDNADLFWALRGGGGNFGVVVEFTFQLYPVGMLLGGHLDYALED
                     APTVLSAWRDLMAKAPDSLASFAQIYRDRATGEGLVNASVAWVGDLDEGRDAVRELTD
                     GLSPVVNSVRPMYYSELQDIYGRMPFGLRNYWSGRFLGELPDELLAQTNEQFLERETY
                     GGVLFEPLHGAPTRVASDATAFAGREGRWNATFIGVWTEAEEDESQIETARAYSRSLD
                     PWRIGGGYLNYASETSADGLETEFGAERFQRLRAVKRQYDPSNVFRFNHNIAPD"
     gene            complement(2371..3633)
                     /locus_tag="VF225_06965"
     CDS             complement(2371..3633)
                     /locus_tag="VF225_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HEX7145035.1"
                     /translation="MTGIGYGRQRQADIYVAGVRGRKPRVPQVAAALEQEAERALSEE
                     GFAYIAGGAGLETTMKANRAAFERVRIVPRMLRGPATRKLEVELFGRTLSAPLLLAPI
                     GVLEMAHRDGDVAVARAAAAEGVPMIFSSQASRPLEECAAAMGDAPRWFQLYMSTSDE
                     LVRSFVSRAERAGCEAIVVTLDTTMLGWRLRDLDLGFLPFLAGKGIAQYVTDPVFQRL
                     VDEAPPPERGPVTPAAIRALWRLARAYPGSTWANLRSPRPRQAVRTFLEVFPRPSLNW
                     EDLAIVRDATKLPILLKGILHPDDARRALDEGVDGIVVSNHGGRQVDGSIGSLDALPA
                     IVEAVGGRAAILLDSGIRGGADVFKALALGATAVLIGRPYCYGLAIAGESGVREVIQN
                     FVADFDLTLGLSGCAAVAEIGGELVEIA"
     gene            3673..4365
                     /locus_tag="VF225_06970"
     CDS             3673..4365
                     /locus_tag="VF225_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7145036.1"
                     /translation="MFLVIQLAALALGGTASCPSNLANDLAAPPPPASSQLITIEAPS
                     ARATYATARTWSRVDGCWTPTDGPYTARVGRNGVRKNKREGDGATPAGTFRVGARMYG
                     NSPSPGVSYPYVRLRCGDWWVEDSKSPAYNTFQRIGCGRKQPFKVTTPDLSTSPRAYA
                     HLAVVGYNMHPVVPGRGSGIFLHVQIGKATSGCISLHRSVLVHVLRWLDPGAGPQIAI
                     GTAASLRQARGG"
     gene            complement(4342..5388)
                     /locus_tag="VF225_06975"
     CDS             complement(4342..5388)
                     /locus_tag="VF225_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX7145037.1"
                     /translation="MATFEELLDRWDGEQAVIHRDRESDGWIFICMHSTQLGPAAGGT
                     RLKVYDTPADGLRDAMRLSAGMTAKLAVADLGLGGGKAVLAVPKVPEGEERTALLHRY
                     GAIVASLGGGFITSSDVGTGETDMDVIAERTEHVFGRSRAKGGAGDPGPFTAVGVFHG
                     IKASLAHVYGSDDLAGRTVLVQGAGSVGGALAERLGHDGATVLVADVDAQRAAGVASA
                     AGGSVVDAGTALETECDVYAPCALGATLSAASIPRLRCRIVAGSANNQLARPEDSELL
                     QSAGILYAPDYVINAGGAIAINYLELNDRSQAEVDAALARIGDTLKEIYTQAEASGLT
                     TAAAADALAASRLS"
     gene            5469..6173
                     /locus_tag="VF225_06980"
     CDS             5469..6173
                     /locus_tag="VF225_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7145038.1"
                     /translation="MKLARLALLAAAGVAIAAFAGVFQPSGAHSAPADTSTGGITVLG
                     TGSTNVIPNRASFSFGTVSQAATANAALAASSQSVARVIAALKKAGLAQADIQTAEVS
                     LSPRMNNNGDEIVGYTASNTVTATIRKIGDAGDVVDAAVGAGANQVSGPNLLASDQDA
                     AYRNALKEAVADARTKAETLATASSSTLGRVTAIVEGGGAIPMPLAAGAKDSSVPIEP
                     GTQNIEATVSVTFALG"
     gene            6190..6462
                     /locus_tag="VF225_06985"
     CDS             6190..6462
                     /locus_tag="VF225_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX7145039.1"
                     /translation="MCRNIHTLYNFEPPATNEEIRDASLQYVRKISGFTKPSQANAEA
                     FARAVDEVAAASKRLLDQLVTSAAPKDREVEAERARARAAKRFATA"
     gene            complement(6473..7012)
                     /locus_tag="VF225_06990"
     CDS             complement(6473..7012)
                     /locus_tag="VF225_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145040.1"
                     /translation="MKAPLVLLTALVVLLAAAGASHLQAAPSRSQAASPFLPTPPLSG
                     PRQIVIYGHVKSVKPKGAGFEVRVDPAEYLSGETANLAAIADKVIPPGDVVPNDHYIR
                     EDGHKLLTFKVPANAHVTVVINKGTAGFTAGLISVSELAEIVKGKNPKHRKLFEPRNA
                     FWIRVASDTALSFDQQYSP"
     gene            7085..7627
                     /locus_tag="VF225_06995"
     CDS             7085..7627
                     /locus_tag="VF225_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145041.1"
                     /translation="MVEEARLEETEGGLAPATEGWFVVNVRDGRWVTNDVLGDAFILE
                     GDDVSFPQVGFTIGVLQPGQSGGRYHRESAQEDFLVLAGECLLLIEEEERPLKAWDFV
                     HCPPDTNHSFVGTGDGPCVIFMTGARIENKETVYPRSELAIRHGAGVETDTSSPAEAY
                     APFPKWKDGRPKTWDGLPWG"
     gene            7630..8232
                     /locus_tag="VF225_07000"
     CDS             7630..8232
                     /locus_tag="VF225_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7145042.1"
                     /translation="MLLYDSPVSGNCYKVRLLLAHLGVEYERRTMDVVDRSNRSEVLG
                     GLNPALRVPTLVLDDGRPLAESGAILWYLGEGTRFVPEDAYERAQVLQWMFFEQYDHE
                     PAIAVARFWLAYSGRPDEFADRLPERTAAGHRALAAMERHLDGRQFLVGDDLTLADIA
                     LYAYTHVAGEGGFDLERYPALRAWLERVAAEPGHATIDAS"
BASE COUNT         1418 a         2832 c         2751 g         1292 t
ORIGIN      
        1 cagcacctga acgtcctgaa ggaggcgggg ctcgtgagcg agcgacgcaa cggcacgagg
       61 cgcctgtacc tggcgcgtcc cgaagggctc gccgagctca aggacttcct ggaggagttc
      121 tgggacgtcc ggctcgagac actgaaacgc gaagcggaaa aggaggagcg aaagaagcat
      181 ggacgcaacc actgagacaa tttcggtcga gcgcacgatc tcgatcgcag ccaccccgga
      241 gaccgtctgg gagttcttcg tcgaccccga gaagctcacg cgctggaaag ggatgaaggc
      301 ggagctcgac ccacgccgcg gcggcgtcta cctctgcgag gtcattcccg gacacactgc
      361 ccgcggcgaa tacgtcgagc tcgacccgcc acgccggctc gtcttcacct ggggctggga
      421 caacgaggaa ggcgtgccgc ccggctcgtc gacgatcgag gtcgagctga cgccggaagg
      481 cgaaggcacg agcctgcact tcgtccaccg cgatcttccg agtgccgagt cagccgagtc
      541 gcacgcgcaa gggtgggatc actacctccc acgcctcgag atcgtcgcga ccggaggcga
      601 ccccggcgag gacccgtggc tgacccaaga catgcaagcc agctagctga gagaggagaa
      661 acgcaatggc acatccggtt atgcacttcg aggtcaccgg caaggacctc gacaagctcc
      721 agagcttcta cggcgagctc ttcggttgga acacgtcgaa gatcccgggc gagatgccgt
      781 acgcgatggt cgagaaggag ggcgacggca tcggcggcgg catcggtcag acgcagaacg
      841 gcaacggcca cgtcaccttc tacgtcggca ccgacgatcc gcaggcgacg ctcgacaagg
      901 ccgagcggct cggcggcaag acgatcatgc cggtgaccga attgccggag gtcacgatcg
      961 cgctcttcgc cgatcccgaa ggacacgtag tcgggctcgc caaggggatg tagcagcccg
     1021 ctagtccgga gcgatgttgt ggttgaacct gaagacgttc gacgggtcgt actgacgctt
     1081 cacggcccgt agtcgttgaa agcgctccgc gccgaactcc gtttcgagac cgtccgccga
     1141 agtctcactg gcgtagttga ggtagccgcc gccaatccgc cacgggtcga gcgacctcga
     1201 gtatgcacgg gcggtctcga tctggctctc gtcttcctca gcctcggtcc agacgccgat
     1261 gaaggtcgcg ttccagcggc cttctctccc ggcgaacgcg gttgcatctg aggcgacgcg
     1321 cgtcggagcg ccgtgaagcg gttcgaagag gacgccgccg taggtctcgc gctcgagaaa
     1381 ctgctcgttc gtctgcgcga gtagctcgtc aggaagctcg ccgagaaacc ggcccgacca
     1441 gtagttccgc agaccgaacg gcatgcgtcc gtagatgtcc tggagctccg agtagtacat
     1501 cggcctgacg ctgttcacca caggcgacag cccgtccgtg agctcgcgga cggcgtcgcg
     1561 cccttcgtcc agatcgccga cccaggcaac cgaggcgttg acgagtcctt ctccggttgc
     1621 gcggtcacgg tagatctgcg caaagctcgc caggctgtcc ggtgcttttg ccatcaggtc
     1681 tcgccacgcg gaaagcacgg tcggtgcgtc ctcgagggca tagtcgaggt gcccgccaag
     1741 caacattccg accggataga gctggaaggt gaactcgacc acgaccccga agttgccgcc
     1801 gcctccgcgc agcgcccaga acaggtcggc gttgtcgctc tcactcgcgg tgagcacgct
     1861 gccgtcggcc gtgacgaccg tcgcagcgag gaggttgtcg agcgtgagcc cgtacgaagg
     1921 tgtgagatgc ccgatgccgc cccccagcgt caggcccgcg atcccggtgt cgccgaaggt
     1981 gccgcccggg gtcgcaaggc cgtgccgttg agccggcgtg tcgaggtcct cccagagcgc
     2041 tcctccaccg cacgtcgccg tccgcgactc cggatcgacg acgacctcgc gtaggaggcg
     2101 caagtcgacc acgaggctcc cgtcgccgat gcagtgacct gcaacgccgt ggcccccgcc
     2161 gcggacggcg accggcagat cggtttcacg cgcaaaccgg acagccgtga cgacatcaac
     2221 cacatcgaga ggtcgcacga tcagctcggg atgccgttcg atcattccgt tgaacgtggc
     2281 gcgggcgacg tcgaagccac ggtcgtcggg agcaatgacc ggtccccgat gcagctctct
     2341 cagctcggtc agcgacgaca cgcgccgacc ctacgcgatc tcgacgagct ccccgccgat
     2401 ctcggcgacc gccgcacagc ccgagagccc aagcgtcaga tcgaagtcgg cgacgaagtt
     2461 ctggatcacc tcgcgcacgc ctgactcccc tgcgatcgcc agcccgtagc agtaagggcg
     2521 cccgatcagc actgcggtcg caccgagcgc cagcgccttg aagacatcgg cgccgccgcg
     2581 gatgccgctg tccagcagaa tcgcggcacg gcccccgacc gcctccacga tcgccggcag
     2641 agcgtcgaga cttccgatcg agccgtccac ctggcgccca ccgtgattcg aaacgacgat
     2701 cccgtcgact ccctcgtcga gtgcgcgccg cgcgtcgtcc ggatggagga tgcccttgag
     2761 caggatcggc agcttggtcg cgtcgcgaac gatcgcgagg tcttcccagt tcagcgacgg
     2821 ccgtgggaac acttcgagga acgtgcgcac cgcctgccgc ggtcgcggcg agcgcaagtt
     2881 cgcccaggtc gagcccgggt aggcccgcgc caaccgccaa agcgcgcgga tggctgcagg
     2941 cgtcaccggt ccgcgctcgg gtggcggcgc ctcgtcgacg agccgctgga acaccgggtc
     3001 ggtgacgtac tgcgcaatgc ccttgcctgc gaggaacggc agaaagccga ggtcgaggtc
     3061 gcgcaaccgc cagccgagca tcgtcgtgtc gagcgtgacg acgatcgcct cgcagccggc
     3121 gcgctcggcg cgtgatacga agctccgcac gagctcgtcg gacgtgctca tgtagagctg
     3181 aaaccaccgg ggtgcgtcgc ccatcgccgc cgcgcattcc tcgagcggcc tcgacgcctg
     3241 gctggagaag atcatcggaa cgccttctgc ggcggccgcg cgcgccaccg ctacgtcgcc
     3301 gtcacggtgc gccatctcga ggacgccgat cggtgcgagc aacagcggcg cagaaagcgt
     3361 acgaccgaag agctcgacct cgagcttgcg cgtagccggc ccgcgcagca tccgcggaac
     3421 gatccgcacg cgctcgaacg cggcgcggtt cgccttcatg gtcgtctcga ggcctgcacc
     3481 gcccgcgatg taggcgaagc cctcttcgct cagcgcgcgc tcggcctcct gctcgagcgc
     3541 cgctgcaacc tgcgggacgc gcggcttccg tccgcgcaca cccgcgacat agatgtcggc
     3601 ctgacgctgc cggccgtaac cgattccggt caccgggccc attctttcgg ctacggcggg
     3661 tacgttgccg ccgtgttcct cgtaatccag ctcgccgcgc tggcacttgg tggtacggcg
     3721 tcctgtccgt cgaacctggc gaacgacctc gcagcgcctc ctccgccggc ttcgagccag
     3781 ctgatcacca tcgaggcccc gagcgcccgc gccacctacg caaccgcacg gacctggtca
     3841 cgcgtcgacg gctgctggac gccgaccgac ggcccgtaca cggctcgcgt cggccggaac
     3901 ggcgttcgca agaacaaacg cgagggagac ggcgcgacac ccgccggcac ctttcgggtt
     3961 ggtgccagga tgtacggcaa ctctccgagc cccggcgtct cgtatcccta cgtccgtctg
     4021 cgctgcggcg actggtgggt ggaggactcg aagtcgcccg cgtacaacac gttccagcgc
     4081 atcggctgtg gccgcaagca gccgttcaag gtgacgacgc ctgacctgtc gacgtcaccg
     4141 cgcgcgtacg cgcatcttgc cgtggtcggg tacaacatgc atcccgtcgt gcccggacgc
     4201 ggttccggca tcttcctcca cgtccagatc ggcaaagcga cgagcggctg catcagcctg
     4261 catcgctcag ttctcgttca cgtgcttcgc tggctcgacc ccggcgccgg tccgcagatc
     4321 gcaatcggta ctgctgcgtc gttacgacaa gcgagaggcg gctagcgcat ccgccgctgc
     4381 tgccgtcgta agcccgctcg cctcggcctg cgtgtagatc tccttcaacg tgtcgccgat
     4441 cctcgcgagc gcggcgtcga cctcggcctg gctgcggtcg ttcagctcta ggtagttgat
     4501 cgcgatcgcg ccgccggcgt tgatcacgta gtcgggcgca tacaggatgc ccgcggactg
     4561 cagcagctcg gagtcctcgg gtcgggcgag ctggttgttc gccgagccgg cgacgatccg
     4621 gcaacggagc cgcggaatgc tggcggcgct cagcgtcgcg ccgagcgcac agggtgcgta
     4681 gacgtcgcac tcggtttcca gtgcggtgcc cgcgtctacc accgagccgc ccgccgcgga
     4741 ggcgactccg gccgccctct gcgcgtcgac gtcggccacc aggacagtcg cgccgtcgtg
     4801 gccgagccgc tccgccagcg ccccgccgac gctgcctgcg ccctgcacga gcactgttcg
     4861 gccggccagg tcgtccgatc cgtagacgtg cgcgaggctc gccttgattc cgtgaaagac
     4921 accaacggcc gtgaacggcc ctggatcgcc ggcgccgccc ttcgcccggc tgcggccgaa
     4981 gacgtgctcg gtcctctcgg cgatcacgtc catgtcggtc tcacccgtgc cgacgtcgga
     5041 gctcgtgatg aacccgcctc ccaatgacgc cacgatggct ccgtaacgat gcagcaacgc
     5101 ggtgcgctcc tcgccttccg gaacctttgg caccgccagc acggccttgc cgccgccgag
     5161 ccctaggtcc gcaactgcaa gcttcgcggt catcccggcg gagaggcgca tcgcgtctcg
     5221 aagcccgtct gcgggagtgt cgtagacctt gagtcgcgta ccacccgccg cgggaccgag
     5281 ctgcgtcgag tgcatgcaga tgaagatcca gccgtcgctc tcgcggtcac ggtggatgac
     5341 ggcctgctcg ccgtcccagc ggtcgagtag ctcctcgaag gtcgccacga cagaaattcg
     5401 atcacatcgt gtgaggatcg cgagggtctg cgacgtacta cgtcacagac aaccgaaggg
     5461 agtgtccgat gaagctcgct cgtctcgccc tgctcgccgc tgccggcgtc gccatagccg
     5521 cgtttgccgg agtcttccaa ccgtcaggag cgcacagcgc cccggccgat acgtcgaccg
     5581 gcggcatcac cgtcctcggt accggatcga cgaacgtcat cccgaatcgg gcttccttct
     5641 cgttcggaac cgtgagccag gccgcgacgg cgaacgcggc actcgctgcg agctcccagt
     5701 cggttgctcg cgtgatcgct gcgctcaaga aggcgggcct ggcccaggcc gacatccaga
     5761 cggccgaagt ctcgctctcg ccgcggatga acaacaacgg cgatgagatc gtcggctaca
     5821 ccgcctcgaa cacggtgacc gcgacgatca ggaagatcgg ggacgccggc gacgtcgtcg
     5881 acgcggcggt cggtgcaggc gcgaaccagg tctcgggccc gaacctgctc gcgtccgatc
     5941 aggacgcggc ctatcgcaac gccctcaagg aagcagtcgc agacgcccgg acgaaggcgg
     6001 agacgttggc gactgcgtcg agctcgacgc tcggcagggt cactgcgatc gtcgaagggg
     6061 gcggagcgat cccgatgccc ctcgcggcgg gcgccaagga ctcgagcgtg ccgatcgagc
     6121 cggggaccca gaatatcgag gcgaccgtga gcgtgacctt cgccttgggg taattcgtag
     6181 gatgtgggca tgtgccgaaa catccatacg ctctacaact tcgagccacc cgccactaac
     6241 gaggagatcc gcgacgcctc gctgcagtac gttcgcaaga tcagcggctt cacgaaaccg
     6301 tcgcaggcga acgcggaagc tttcgcccgc gcggtcgacg aggtcgctgc agcgtcgaag
     6361 cggttgctcg accaactcgt gacgtccgcg gcgccgaagg accgcgaggt cgaagccgaa
     6421 cgggctcgcg cccgagcggc caagcgcttc gctacggcct agccggccag agctacggcg
     6481 agtactgctg gtcgaatgaa agtgccgtgt cgctcgcgac ccggatccag aacgcattcc
     6541 gcggctcgaa cagcttgcgg tgcttcgggt tcttgccctt gacgatctct gcgagctcgg
     6601 agacggagat caatccggcg gtgaagccgg cggtgccctt gttgatgacc acggtgacgt
     6661 gggcattcgc cggcaccttg aaggtgagca gcttgtgccc gtcctcgcgg atgtagtgat
     6721 cgttcggcac gacgtcaccc gggggaatca ccttgtccgc gatcgccgcg agattcgcgg
     6781 tctcgcccga caggtactcg gctggatcca cgcgcacttc gaagccggcg cccttcggct
     6841 tgaccgactt gacatgcccg tagatcacga tctgccgcgg tccgctcagg ggcggcgtcg
     6901 gcaggaaggg agaggcggcc tgcgagcgag agggcgcggc ctgcaggtgg gacgcaccgg
     6961 ccgcggccag aagaaccacg agcgcggtga gcaggaccag cggggccttc aagattctcc
     7021 cttcgaaggg gacagcgact gttcgattat cgtggccgga agttaacggg aggtgaggag
     7081 cgacatggtc gaagaagcgc ggctcgaaga gaccgaggga ggtctcgcgc ccgcaacgga
     7141 gggctggttc gtcgtgaacg tccgcgacgg tcgctgggtg acgaacgacg ttctcggaga
     7201 cgctttcatc ctcgagggcg acgacgtctc gttcccccag gtcggtttca cgatcggcgt
     7261 cctgcagccg ggccagtcag gcgggaggta ccaccgcgag tccgcgcagg aagacttcct
     7321 cgtcctggcc ggggagtgcc tgctcttgat cgaagaggag gagcgcccgc tcaaggcgtg
     7381 ggacttcgtc cactgcccgc ccgataccaa ccactcgttc gtcggcacag gcgacgggcc
     7441 gtgcgtcatc ttcatgaccg gagcgcgcat cgagaacaag gagaccgtgt atccgcgctc
     7501 ggaactggca atccgtcacg gcgccggcgt cgagacggat acgagctcgc cggccgaggc
     7561 ctacgcaccg ttcccgaagt ggaaggacgg acgcccgaag acctgggacg gacttccctg
     7621 gggctagcgg tgctgctgta cgacagccca gtctccggca actgttacaa ggttcggctc
     7681 ctgctcgcgc atctgggcgt tgagtacgag cgccgcacga tggacgtggt cgaccgctcg
     7741 aaccggtcgg aggttctggg cggactgaac cctgcgctgc gagtgccgac gctggtgctg
     7801 gacgacggca ggccgctcgc ggagtcgggc gcgatcctct ggtacctcgg cgagggcacg
     7861 cgtttcgtgc cggaagacgc ttacgagcgc gcacaggtcc tgcaatggat gttcttcgag
     7921 cagtacgacc acgagccggc gatcgcggtt gcgcgtttct ggctcgcgta ctccggccgc
     7981 ccggacgagt tcgcggatcg gctccccgag cgcaccgccg ccgggcacag ggcgctcgcg
     8041 gcgatggagc gtcacctcga cgggagacag ttcctcgtcg gcgacgacct gacgctcgcg
     8101 gatatcgcgc tctatgccta tacgcacgtt gcgggcgagg gcggcttcga cctggagcga
     8161 taccccgcgc tgcgtgcatg gctcgagcga gtggctgccg agcctggaca cgccacaatc
     8221 gacgcgtcat gaagatccgg gcagccctcc tcgaagactt cggtgcgccg ctgtctgttc
     8281 aggacctcga cct
//
LOCUS       DASVEU010000173        11495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1090946,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000173 DASVEU010000000
VERSION     DASVEU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090946"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VF225_07005"
     CDS             complement(<1..1368)
                     /locus_tag="VF225_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7145043.1"
                     /translation="MTQRIAFLLGVLLCGLALPETGWAHARLLRTVPGDGSVLGSAPG
                     RVRVVFDDNVRPVSGIKAIRNGGGSVLGGKPRVVGGRVLVVPLRTGLGDGDYTVLWRV
                     LSDDGHTVSGVLAFGVGAGRAPPQAALSAGNGPSAQDVVSRVLFFAGLLTAAGAACFR
                     FAVGRVPLRLMLGAFLLVFIGASGMAHDVSLSTRFGTVIAVAAVVSGLGAVLAAVAPL
                     LPWLEPLPFLVGLALLPLPTLAGHSLDRGRPLLEPAVDFLHVAAASFWLGGLVALALA
                     LTGSGDRTRMLRRFSNVALVSVAVLAATGVIRAFAELSSLGQLWSTGYGRVLIVKTAL
                     LTALVTIGWLNRYRLVPRLSLEGLRRNVAVELALFAALVAAVALLTDLRPGRDHIARA
                     AVVEAKGPPPSPAAGMWVQARESGAFGVALAVQPPNAEVLVIGQDNLGVNGLAVKVAG
                     VPARSC"
     gene            1412..2728
                     /locus_tag="VF225_07010"
     CDS             1412..2728
                     /locus_tag="VF225_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7145044.1"
                     /translation="MLFRQFVDDDLGCASYLIGDPSKGEAFLVDPAYAIEQYLEAADE
                     AGVRIVRVLETHTHADHVSGHGRFALEHGVPVSVHPLAAPDFTFDPLRDGQDLPVGSV
                     PVRVIHTPGHRPEHCAFVVDDRVVLTGDSLFIGDAARPDLAVEAREGAEGLFHSLRRL
                     TQLPDAYEVYPGHVAGSLCGAAMSPDHSSTIGNEKRTNAKLEYGDAQSFINATASLST
                     PRPPTTARVVALNKGPFVGAPPPLDHLEEPGGATILDVRPAELHAAGHVRGAFNVPVS
                     GSSFATRAGFVLDPEERIVIHASDKAEASEAARGLCAVGFLELAGYVTDVPMTEKVEP
                     MGLDELEQLLEQGAVQVLDVREKAERDEGYIPGSLHIPFRLLRGFGTEGVNTEKPVVT
                     ICESGMRASIAASVLTAVGVDARPVLHGGVHDWPGGTVSFRRCGSG"
     gene            complement(2806..3978)
                     /gene="ilvA"
                     /locus_tag="VF225_07015"
     CDS             complement(2806..3978)
                     /gene="ilvA"
                     /locus_tag="VF225_07015"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX7145045.1"
                     /translation="MAIKQIAPTLADIQEARQRLRGIAEQTPIYLSETFSRRSGREVR
                     LKAENLQRTGAFKIRGAVNKLSTLSPEERADGVVAASAGNHGQAVAWAARELGIRARI
                     FVPDTAPMAKVEACKNYGAETELTGSFFEDAMVAALAYVEETGGTFIHPYEDRLVVAG
                     QGTVGLELLEQEPEVGTVLIPIGGGGLALGIATVLRALRPEVRIVGVQAGVDGYTIAD
                     GIAVKVPSELTMPLLEDLLDDLVSVTDEEISEAIVLLLERAKLVVEGAGAVGVAALLA
                     GRAGGSGMAIPILSGGNIDPTMLISVMRHGLTAAGRYLVMRTKLPDRPGELIKLLSLV
                     AEERGNVIAVEHHREGVDISVSETEIGLTLVTRDEAHCQQILAAMKDRGYTVERLS"
     gene            complement(3944..4939)
                     /gene="meaB"
                     /locus_tag="VF225_07020"
     CDS             complement(3944..4939)
                     /gene="meaB"
                     /locus_tag="VF225_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7145046.1"
                     /translation="MHGRRDWTRESLAEGVRTGDTRALARAISLVENGDPLAYDLVRD
                     IYPETGRTYAVGITGPPGVGKSSLISTLIGHIREQDRTIGVISVDPSSPFTQGALLGD
                     RIRLSDHFLDPGVFIRSMGTRGHLGGLAETTLQALLILDAAGKELVFLETVGTGQSEV
                     EVIGIADTVVLVLMPGSGDSVQALKAGIMEIPDVIAINKMDHPAAKTMLNEVRSILSL
                     DRESSWKPPIVLTEATRGENVAELWAKIEEHRAYLESEGRLEERRRKNLAGEVFAVAS
                     GRAKAHLQQAVANDPELGRLLDEVQRRELDPLTAVKEIMEKVFKLGDKADRTDTR"
     gene            complement(4943..6178)
                     /locus_tag="VF225_07025"
     CDS             complement(4943..6178)
                     /locus_tag="VF225_07025"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145047.1"
                     /translation="MTSLRRQLSLLATAPGFRLLFLATLGSSLGTLLATVALVVDIKD
                     RTNSGSWVSALMIVEFLPAVAVGLFLGPLVDRISRRGLMIVSDLVRAGAFFTLPFANT
                     AGQIVALAGIAGLATAFFRPAVYAGLPNLVDEQELPRANSLIQTSENVSWTVAPIIGG
                     AIVAASGPELAYWLNGASFLVSVLLLLRLPAERLQGALAVSRGHLRDLKDGFARVIRT
                     RSLLTVLVVWTIVLGAVASTQTAQVFLAKDAFSAGDFGYGLIFGCIGLGLAVGSFGAG
                     TWLERRPVGTVYAGSILLQAVGVAAAAVAPNVWTSLPFFVLAGVGNGTAVVCNSLLVQ
                     RGAPDAIRGRVFTVIMSVNYAVYGLGFVVAGPLTDSVGPRWVFGGVGVVLAGASLVAL
                     ALARGAEQEVDRTAEAEAA"
     gene            complement(6193..7314)
                     /gene="hisC"
                     /locus_tag="VF225_07030"
     CDS             complement(6193..7314)
                     /gene="hisC"
                     /locus_tag="VF225_07030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7145048.1"
                     /translation="MADIPAGLLRPALAGIIPYEPGKPVEEVQRELGLERVVKLASNE
                     GPFGPFPQALEALKRSAGDLNRYPDGGAYRLRVALAERHGVRFEEIAIGGGADGLIDG
                     ISQAVLDEGDQVVSGWPSFPSYAIYARKLGAQAIQVPLRENRYDLDALLDAITERTKL
                     VYVCHPNNPTGTMSSRAELDAYFARAPDHVLTVLDQAYFEYVDDPDYADGIEEYFKSG
                     RQVIVLRTFSKIYGLAGARVGYGVGPAELVVALGKTRRAFDLTSPAQEAALASLDSPG
                     ELERRKQANAEGRAELERILTEAGYKVTGPAVGNFVFVDLGEDAQPWFEALLREGVIV
                     RPLAGFGAPQAMRVTVGTAEDHAFLAEALVRLRTATTAS"
     gene            complement(7314..7526)
                     /locus_tag="VF225_07035"
     CDS             complement(7314..7526)
                     /locus_tag="VF225_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145049.1"
                     /translation="MGIINRRNAVLGWGVWKVAKKFGKRKARAAVPGTGEHAGLNKGA
                     IASMLAAAGGALWFWRKKSDETTPAS"
     gene            complement(7609..8799)
                     /locus_tag="VF225_07040"
     CDS             complement(7609..8799)
                     /locus_tag="VF225_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145050.1"
                     /translation="MTPLLAQRPRSDRAFERLYKRHVGDVYRYALAVMRNPTDAEDVT
                     QTTFLNAYRSYVEKGSRPEKPQNWLIAIAHNVCRQRFRQSARRPAEVSFEDDIADTVA
                     DDDAPSGDDIRRALSHLAFNQRAALVMRELEGRSYAEIAEILEVSTSAVETLIFRARR
                     ALREQLEGSLTCGEAEFAISRQLDGRLPRGERGQLRAHLRECSECAGFARRQRAQRGA
                     LKTLALVPVPSSLMSLFGGSSGTAVGTGAAVGTGLTLKAAAAVTAGLAIGGAGYEGVR
                     HVPWHTQKPTAAAGKNAVGAVAAPRDAALSPRGPSRVVAAASPRKHGHLTKTKTVNVK
                     RGKKAKTHGRANHGFGAAQSGRAHAAGGPRVHQKVKLKPKRLRLTRPTRERPAHARRN
                     AKAK"
     gene            complement(8857..9972)
                     /locus_tag="VF225_07045"
     CDS             complement(8857..9972)
                     /locus_tag="VF225_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7145051.1"
                     /translation="MATAAPEIPVLARDQAFETLYRRYVKDVYHYALALLRNPADAED
                     VTQTTFLNAYRAYQRGIEIEKPHNWLIKIAHNVARTRYARAGRRVKEVPLDDHVEQLA
                     VPEAEQSDVVGVLRALGRLPFNQRAALVMRELEGRTYAEIADTIGVSVSAVEALIFRG
                     RKSLRLNASALRSLAVVPLPTSLTQFFGEGGMIAGGGAAVGTGFLLKAATAIVAGALA
                     TGVASDHGRLGTAAAHPSTGSAVATSGKGGADGLKAQVRKLAVRGGHSAAKKGSPAGV
                     ATGRAGDLTTVGAGTQHTSAGQPASPASTAQASSTLSGPVPQVTAPVSDLTSSVQQVV
                     EAPTAALPVQAPSLPATPVDLPKAPVLPPPPPLPPLP"
     gene            complement(10046..11452)
                     /gene="lpdA"
                     /locus_tag="VF225_07050"
     CDS             complement(10046..11452)
                     /gene="lpdA"
                     /locus_tag="VF225_07050"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7145052.1"
                     /translation="MEVDVAVLGGGPGGYTAAIRAAQLGGRVACVEREPELGGTCLRI
                     GCIPTKAWVQTAFALKEAEHTFDKLGVKLGGFQLEFGTANEWKAGVVKQMTSGVASLF
                     KANGVEWVKGNGTFKDAHTLAVEGGEDITFKQAIIATGSFPLRPPIEGLDSERCVDSE
                     GLLAQTEVPKRLVVLGGGIIGCEFASIFQRFGSEVTIIEMLPALIPQEDADASKELAK
                     QFGKRGITLHLEKQCKKVEDTGGALTVHFGDGETVQADLMLVSVGRGPSVEGVGLEAI
                     GVEFDKRKGIAADEHRRTTVEHIYAVGDCAGYWQLAHTAFREGEVAAENACGHEAVVD
                     NRAVPRPIYTDPEIAGVGLTEAEAREQYGDDVATGMFPWVANARAVMQNETTGWVKSI
                     HETRYGELLGLVMVGPHVTDLIEAGVVAIDAEATVETVADGMAAHPTLSEAIKEAGLV
                     ALGRAIHVPNRKRAKTPA"
BASE COUNT         1853 a         4061 c         3769 g         1812 t
ORIGIN      
        1 gcaagagcgg gccggaacgc cggcaacctt gaccgcgagg ccgttgactc ccaggttgtc
       61 ctggccgatt accaacacct ccgcgttcgg tggctgcacc gcgagcgcaa ccccgaaggc
      121 tccgctctcg cgcgcctgta cccacatccc cgctgcaggc gacggcggcg gacccttcgc
      181 ctccaccacg gccgcccgcg cgatgtggtc gcgcccgggc cgaagatccg tcagcagcgc
      241 gacggcggcg acgagcgcgg cgaacagcgc caactcgacg gcgacgttcc gccgcaggcc
      301 ttcgagggac aaccgcggga cgagccggta ccgattgagc cagccgatgg tcacgagagc
      361 cgtgagcagt gccgtcttca cgatcagcac ccgcccgtag ccggtcgacc acagctgtcc
      421 gagcgagctc agctccgcga atgcccgaat caccccggtc gctgccagca ctgccaccga
      481 caccagcgcg acgttggaaa agcggcgcag catccgagtg cggtcgcccg agcccgtgag
      541 cgccagcgcg agcgcgacga gtccgccgag ccagaacgac gccgccgcga cgtgcaggaa
      601 atcgaccgcc ggctcgagca gcggcctgcc gcgatcgagc gagtggcccg cgagcgtcgg
      661 cagcggcagc aacgccaggc cgaccaggaa cggcagcggc tcgagccacg gaagcagagg
      721 cgccacggcg gcgaggacgg cgccgagccc cgagaccacc gccgccacgg caatcacggt
      781 gccgaagcgc gtcgacagcg agacgtcgtg cgccatgccc gacgccccga tgaagacgag
      841 caggaaagcg ccgagcatca gccggagcgg gacgcggccg accgcgaacc ggaagcatgc
      901 cgcgcccgcc gcagtcagga ggccggcgaa gaagagcaca cgggagacga cgtcttgcgc
      961 gctcggaccg ttgccggcgg agagagccgc ctgcggtggg gcccggccgg cgcccacacc
     1021 gaaggcaagc acgcctgaga ccgtgtgacc gtcgtcggag aggacgcgcc acagcaccgt
     1081 gtagtcgccg tcgcccaggc cggtccgaag cgggacgact agcacgcggc cgcccacgac
     1141 gcgcggcttt ccgccgagca ccgagccgcc cccgtttcgg atcgccttga tcccggagac
     1201 agggcgaacg ttgtcgtcga agacgacgcg gacccgcccg ggagcgctgc cgagcacgct
     1261 tccgtccccg ggaacggtgc ggagcagcct ggcatgggcc cagccggtct cggggagagc
     1321 caggccacag agcaggacgc cgaggagaaa ggcgatgcgt tgggtcaacg cttcaagaaa
     1381 gctagcggca cgcgctgtta gcttctcggg tgtgctgttt cgccagttcg tcgacgacga
     1441 tctcggctgc gcctcctacc tgatcggcga ccccagcaag ggggaggcct tcctggtcga
     1501 ccccgcgtac gcaatcgagc agtacctcga agcggccgac gaagcgggag tccgcatcgt
     1561 ccgagtgctg gagacccaca ctcacgccga tcacgtctcg gggcacggcc gattcgccct
     1621 cgagcacggg gtgcccgtct cagtccaccc gctggccgca ccggacttca ccttcgatcc
     1681 cctgagggac ggccaggacc tgccggtcgg gtcagtgccg gtccgtgtga tccacactcc
     1741 gggccaccgg ccggaacact gcgccttcgt cgtcgacgac cgggtcgtgc tcaccggcga
     1801 ctcgctcttc atcggggatg cagcacgacc cgacctcgca gtcgaggcgc gcgaaggcgc
     1861 ggagggcctg tttcactcgc tcaggcgact gacgcagctt ccggacgcat acgaggtcta
     1921 ccccggccac gtagccggct cgctgtgcgg tgcggcgatg agccccgatc actcctcgac
     1981 gatcggcaac gagaagcgca cgaacgcgaa gctcgaatac ggcgatgcgc agtccttcat
     2041 caacgcaacc gcctcgctgt ccaccccccg tcccccgacc accgcccgcg tcgtcgcgct
     2101 caacaaaggg ccgttcgtcg gcgcgccccc gccgctcgat cacctcgagg agcccggcgg
     2161 ggcaacgatc ctcgacgttc gccccgccga gctgcatgcc gcaggacacg tgcgcggcgc
     2221 gttcaacgtg ccggtgtcgg gctcatcgtt cgcgacgcgc gcaggcttcg tgctggatcc
     2281 cgaggaacga atcgtcatcc atgcctccga caaagctgag gcctccgagg cggcgcgcgg
     2341 gctgtgtgcc gtcgggttcc tcgagctcgc cggttacgtc accgacgtgc cgatgacgga
     2401 gaaggtcgag cccatggggc tggacgagct ggagcagctg ctcgagcaag gcgcggtgca
     2461 agtgctcgac gtccgcgaga aggctgagcg cgacgagggg tacatccccg gctcactcca
     2521 catcccgttc aggctgttgc gtggcttcgg tacggagggc gtgaacaccg agaagcccgt
     2581 cgtgacgatc tgcgagtccg gcatgcgggc gagcatcgcg gcaagtgtct tgaccgccgt
     2641 cggcgtcgac gcccggccgg tgcttcacgg cggcgtgcac gactggcccg gcggaacggt
     2701 ctcgttcagg cgctgcggct caggctgacg cggcgagcag ggcctcgccg accaggtcgg
     2761 cgaacaggac cgtgacgacc ttgcgctcgc gccgcccctc catgttcagc tgaggcgttc
     2821 gacggtgtaa ccgcggtcct tcatcgcggc gaggatctgc tggcagtgtg cctcgtcgcg
     2881 tgtgacgagc gtcagcccga tctccgtctc gctcaccgat atgtcgacgc cttcgcggtg
     2941 atgctcgacg gcgatgacgt tgccgcgctc ctccgccact agcgaaagca gcttgatcag
     3001 ttcgcccgga cggtcgggaa gcttcgtcct catgacgaga tagcgccccg ccgcagtgag
     3061 gccgtggcgc atgacggaga tgagcatcgt cgggtcgatg ttgccgccgg agagaatcgg
     3121 gatcgccatc cccgagcctc ccgcccgccc ggcgagcagc gccgcgacac cgacggcgcc
     3181 cgcgccttcg acgacgagct tcgcacgctc gagcagcaac acgatcgcct cgctgatctc
     3241 ctcgtccgtg accgagacga ggtcgtccag cagatcctcg aggagcggca tggtgagctc
     3301 gctcggaacc ttcaccgcga tcccgtcggc gatcgtgtag ccgtccacgc cggcctgcac
     3361 tccgacgatg cgcacctcgg gccgtagggc gcgcaggacg gttgcgatac cgagtgcgag
     3421 gccgccacca ccgatcggga tgagcaccgt gccgacctcg ggctcctgct cgagcagctc
     3481 gagcccgacc gttccctgcc ctgccacgac gagtcggtcc tcgtagggat ggatgaacgt
     3541 accgcctgtc tcttcgacgt aggcaagcgc ggccaccatc gcgtcctcga agaacgagcc
     3601 ggtgagctcc gtctcggcgc cgtagttctt gcacgcttcg acctttgcca tcggcgcggt
     3661 gtccggcacg aagatgcgcg cacggatacc gagctcgcgc gctgcccagg ctacggcctg
     3721 cccgtgattg ccggcgctgg ccgcgacgac gccgtcggcg cgctcctcgg gcgagagcgt
     3781 gctcagcttg ttgaccgcgc cgcggatctt gaacgcgccg gttcgctgga ggttctccgc
     3841 cttgagccga acctcgcggc cgctgcggcg cgagaacgtc tccgacagat agatcggtgt
     3901 ctgttccgcg atcccccgaa gacgctgccg cgcctcctgg atgtcagcga gtgtcggtgc
     3961 gatctgcttt atcgccaagc ttgaacacct tctccatgat ctctttcacc gcagtcaatg
     4021 ggtcgagctc tcgccgctgc acctcgtcga gcagacgacc cagctccggg tcgttcgcaa
     4081 ccgcctgctg aagatgggcc ttcgcgcgac cggatgcaac agcgaacact tcaccggcga
     4141 gattcttgcg cctgcgctcc tccagccgcc cctccgactc cagataggca cgatgctcct
     4201 cgatcttggc ccacagctcc gcgacgttct caccgcgtgt cgcctcggtg agaacgatcg
     4261 gcggcttcca gctgctttcg cggtcgagcg acaggatcga gcgcacctcg ttgagcatcg
     4321 tcttcgcggc gggatgatcc atcttgttga ttgcgatcac atccgggatc tccatgatcc
     4381 ctgccttcag ggcctgaacg gagtctccgg atccaggcat caggacgagc acgaccgtat
     4441 cggcgatccc gatcacctcg acctcgctct gacccgtgcc gacggtctcc aggaagacga
     4501 gctccttccc ggcagcgtcc aggatcagca acgcctgcag cgtcgtctca gccagtccgc
     4561 cgagatggcc gcgcgtcccc atcgaccgga tgaacactcc cggatcgagg aaatgatcgg
     4621 agagccggat ccggtcgccc agcagcgccc cctgggtgaa cgggctcgac gggtcgaccg
     4681 agatcacgcc gatcgtgcgg tcctgctcgc ggatgtgccc gatcagcgtg gagatcaggc
     4741 tcgacttgcc gacgcccggc ggtcccgtga ttccgaccgc ataggtgcgg cccgtctcgg
     4801 ggtagatgtc acgcacgagg tcgtaggcga gcgggtcgcc gttctcgacc agcgagatcg
     4861 cccgggcgag tgcccgtgtg tcgcccgtgc ggacgccttc cgcgagcgac tcccgcgtcc
     4921 agtcccgccg cccgtgcatt gactacgcgg cctctgcctc ggcggttcga tcgacttcct
     4981 gctccgctcc ccgggccagc gccagagcga cgagagacgc gccggcgagc acgacgccga
     5041 cgccgccgaa cacccaacgt gggccgacac tgtcggtgag cggccctgca acgacgaagc
     5101 cgagcccgta cacggcgtag ttgacgctca tgatcacggt gaagacgcgg ccgcggatcg
     5161 cgtcgggcgc gccgcgttgc acgaggagcg agttgcagac gactgcggtt ccattgccga
     5221 cgccggcgag cacgaagaac ggcagcgagg tccacacgtt cggagcgacg gccgcagcgg
     5281 cgactccgac tgcctgcagg agaatgcttc cggcgtagac ggtgccgacg ggccgccgct
     5341 ccaaccacgt tcccgcgccg aagcttccga cggcgaggcc gagcccgatg cagccgaaga
     5401 tcaagccgta gccgaaatca cctgcactga acgcgtcctt cgcgaggaac acctgcgccg
     5461 tctgcgtcga agcgacagca ccgaggacga tcgtccagac gacgagcacg gtcagcagtg
     5521 aacgggttcg gatcacgcgc gcaaacccat ccttgaggtc acgcagatga ccgcggctca
     5581 ctgcgagcgc accctgcagc ctctctgcgg ggagacgcaa gagcaggagc acggacacga
     5641 ggaacgatgc gccgttcagc cagtacgcga gctcgggccc ggatgcggcg acgatggcgc
     5701 cgccgatgat cggcgccacc gtccagctga cgttctcgct cgtctggatc agcgagttcg
     5761 cgcgtgggag ctcctgctcg tcgacgaggt tcggcaggcc cgcgtacacc gccggccgga
     5821 agaatgccgt cgccaatccc gcgatccccg ccaacgcgac gatctgtccg gctgtgttcg
     5881 cgaacggcag ggtgaagaag gccccggccc ggacgagatc ggagacgatc atcaggccac
     5941 gccgtgagat tctgtcgacg agcgggccga gaaagagccc gacggccacc gccggcaaga
     6001 actcgacgat catcaaggcg ctcacccacg agcccgaatt ggtgcggtcc ttgatgtcga
     6061 cgacgagggc gacggtcgcg agcagcgtcc ccaaactcga cccgagcgtg gccaggaaca
     6121 acaggcgaaa gccgggtgcg gtggcgagga ggctcagctg gcggcggagg ctggtcaccc
     6181 cggcgcagtc tgtcaggaag ccgttgtggc cgtcctcagc cggacgagtg cttccgcgag
     6241 gaacgcgtga tcctcagccg ttccgaccgt cacgcgcatc gcctgcgggg cgccgaatcc
     6301 cgcgagcggc cgcacgatca cgccttcgcg cagcaacgct tcgaaccatg gctgcgcgtc
     6361 ttcgcccagg tcgacgaaca cgaagttccc gaccgcagga ccggtcacct tgtaccccgc
     6421 ttccgtcagg atccgctcga gctccgcacg gccctcggcg ttcgcctgct tccggcgttc
     6481 cagctccccg ggcgaatcca ggctggcgag cgctgcttcc tgcgccggcg aagtcaggtc
     6541 gaatgcccgc cgcgtcttac cgagcgcgac cacgagctcc gccggtccga ctccgtaacc
     6601 gacgcgggct cccgccaggc cgtagatctt cgagaaggtg cgcaagacga tcacctgccg
     6661 gccggacttg aagtactcct cgatgccgtc cgcatagtca gggtcgtcga cgtactcgaa
     6721 gtacgcctga tcgagcaccg tcagcacgtg gtccggagct cgcgcgaagt atgcgtccag
     6781 ctcggcgcgc gagctcatcg ttccggtcgg gttgttggga tggcagacgt acacgagctt
     6841 cgtccgctcc gtgatcgcgt ccagcagggc gtcgaggtcg tagcgatttt cgcggagcgg
     6901 cacctgaatg gcctgcgcac cgagcttgcg cgcgtagatc gcatagctcg ggaaggacgg
     6961 ccagccggat acgacctggt cgccctcgtc caacacagct tgtgagattc cgtcgatcag
     7021 tccgtcggct cccccgccga tcgcaatctc ctcgaacctc acgccgtgtc gttcggcgag
     7081 tgcgacgcga aggcggtacg ccccaccgtc cggatagcgg ttcaaatcgc cggccgagcg
     7141 cttcaacgcc tcgagcgcct gcgggaaggg gccgaacggc ccttcgttcg aggcgagctt
     7201 gacgacacgt tcaaggccga gctctcgctg aacctcttcg accggcttgc ccggctcgta
     7261 ggggatgatt cctgcgagcg cgggcctgag gaggccggcg ggaatgtccg ccactacgag
     7321 gctggcgtcg tctcgtccga cttcttgcgc cagaaccaga gcgccccgcc cgctgctgcg
     7381 agcatcgacg cgattgctcc cttgttcaac ccggcgtgct cacctgttcc agggacggcc
     7441 gcgcgggcct ttcgcttccc gaacttcttc gcgaccttcc agactcccca accgagcact
     7501 gcgttccgtc gattgatgat ccccatgggt ctcctttcgc gagagcgagc ctaccaagca
     7561 cccttcccaa tcggggccgg gcccaactca cggacgaaga actgggggtt atttcgcctt
     7621 ggcgttcctg cgtgcgtgtg ccggacgctc acgagtcggc ctcgtcagcc gcagacgctt
     7681 cggcttgagc ttcaccttct ggtgcacacg ggggccaccg gccgcgtgcg ctcggccgga
     7741 ttgcgccgca ccaaagccgt ggtttgcgcg gccgtgcgtc ttggccttct ttcccctctt
     7801 cacgttgacg gtcttcgtct tcgttaggtg gccgtgcttc cgggggctgg ccgcggccac
     7861 aacgcgcgag ggtcctcgcg gcgagagggc ggcatcgcgc ggcgctgcaa ctgctccgac
     7921 agcgttcttg ccagccgccg ccgtcggctt ctgcgtgtgc cacggcacgt ggcgcacccc
     7981 ttcgtaacct gcgccgccga tcgccaagcc cgcggtgacc gcggccgctg ccttgagcgt
     8041 caggccggtt cccaccgcgg caccggttcc caccgcggtg ccgctgctac cgccgaacaa
     8101 cgacatcagc gaactcggca ccggcacgag cgccagcgtc ttcagggcgc cgcgttgtgc
     8161 ccgctgccgg cgggcgaagc cggcgcattc cgagcattcg cgcaggtgcg cgcgcaactg
     8221 gccgcgctct ccccgcggaa gccgtccgtc gagctggcgt gaaatggcga actcggcctc
     8281 gccgcaggtg agcgacccct cgagctgctc ccgcaacgcg cgccgcgcgc ggaagatcag
     8341 tgtctccacc gcgctcgtcg agacctcgag gatctcggcg atctcagcgt acgaccggcc
     8401 ctcgagttcc ctcatgacca gcgccgcccg ctggttgaag gcaaggtgac tcaaggctcg
     8461 ccgtatgtcg tcgccggacg gcgcgtcgtc gtcggcgacc gtgtcggcga tgtcgtcctc
     8521 gaacgagacc tcggccggcc gcctcgccga ctggcggaac cgctggcggc agacgttgtg
     8581 cgcgatcgcg atcaaccagt tctggggctt ttccgggcga ctacccttct cgacgtacga
     8641 gcggtaggca ttgaggaagg tcgtctgcgt gacgtcctcg gcgtctgtgg ggttgcgcat
     8701 gacggcgagc gcgtaccgat acacgtcgcc gacgtgccgc ttgtagaggc gctcgaaggc
     8761 ccggtccgag cggggcctct gcgccagcag gggcgtcatg acctagtaga tacccgttca
     8821 gccggaatct tgcgtccccc gtcggaacga aacggcttaa ggcaggggcg gcaggggcgg
     8881 cggtgggggc agaacagggg ctttcgggag gtcgaccggg gtcgcgggca ggctcggagc
     8941 ctgcaccggc agcgccgctg ttggcgcctc gacgacctgc tgaaccgagc tcgtcaggtc
     9001 agagaccggc gcagtgacct gggggaccgg tccggacagc gtgctcgagg cctgggcagt
     9061 cgaggcaggc gaagcgggtt ggccggccga tgtgtgctgg gtcccggcgc cgactgtggt
     9121 gaggtctcct gcgcgcccgg tcgccacgcc cgcgggcgag cccttcttcg ccgcgctgtg
     9181 tccaccgcga actgcgagct tccgcacctg cgccttcagc ccgtccgcgc cccccttgcc
     9241 cgatgttgcg accgcagagc ccgtgctcgg gtgcgcagcg gcggtaccga gacgaccgtg
     9301 gtccgaggcg acgcccgtcg ccagagcccc agcgacgatc gccgtggcgg ccttcagcag
     9361 gaagccggtg ccgacggctg ccccgcctcc cgcgatcatg cctccttccc cgaaaaactg
     9421 cgtcagcgag gtcggcagcg gcacgaccgc aagggaacgg agggccgatg cattgaggcg
     9481 cagcgacttc cgcccgcgga agatcagcgc ctcgacggcc gaaaccgaga ccccgatcgt
     9541 gtcggcgatc tccgcgtagg ttcgaccctc gagctcacgc atgacgaggg ccgcacgctg
     9601 gttgaacggc aagcgcccaa gcgcgcggag aacacctacg acatccgatt gctcagcttc
     9661 gggaaccgca agttgctcga cgtgatcatc caacgggact tccttcacac ggcggcctgc
     9721 gcgtgcatat ctcgttcggg cgacgttgtg cgcgatcttg atcagccagt tgtgcggctt
     9781 ctcgatctcg atcccgcgct gataggcccg gtacgcgttg aggaacgtcg tctgtgtgac
     9841 gtcttccgcg tcagccggat tacgcagcag cgcgagcgcg tagtggtaga cgtccttgac
     9901 gtagcgccgg tacagggtct cgaaggcctg atcgcgagct aggaccggga tctctggcgc
     9961 ggcggtcgcc atgtgctgat gcaacgacta acccgcccag caggatacgg caggttttct
    10021 cattttaaaa cggtatctgg ggcggctacg cgggagtctt cgcgcgcttg cggttcggga
    10081 cgtggatcgc gcggccgagc gccaccagcc cggcttcctt gatcgcctcc gacagggtcg
    10141 gatgggcggc catgccatcc gccacggtct cgacagtcgc ctcggcgtcg atcgccacca
    10201 cgcccgcctc gatcaggtcg gtcacgtgcg ggccgaccat cacgagcccg agcagctcgc
    10261 cgtaccgcgt ctcgtggatc gacttgaccc aacccgttgt ctcgttctgc atcaccgcgc
    10321 gggcgttcgc gacccatggg aacatgcccg tcgccacgtc gtcaccgtac tgctcgcgcg
    10381 cctctgcctc ggtcaggccg acgcccgcga tctccggatc cgtgtagatc ggccgcggca
    10441 cggcacgatt gtcgaccacg gcttcgtggc cgcaggcgtt ctcggccgcg acctcaccct
    10501 cgcgaaacgc ggtgtgcgcc agctgccagt agcccgcgca gtcaccgacc gcgtagatgt
    10561 gctcgacggt ggtgcggcgg tgctcgtctg cagcgatgcc tttgcgcttg tcgaactcga
    10621 cgccgattgc ttcgaggccc accccttcga cgctcgggcc gcggccgacg gagacgagca
    10681 tgagatcggc ttggaccgtc tcgccgtcgc caaagtgcac ggtcagcgcg cctcccgtgt
    10741 cctctacctt cttgcattgc ttctcgaggt gaagcgtgat cccgcgcttg ccgaactgct
    10801 tcgcgagctc cttcgacgcg tcggcatcct cttgcgggat cagcgccggc agcatctcga
    10861 tgatcgtcac ctcactaccg aaccgctgga agatcgaggc gaactcgcag ccgatgatgc
    10921 cgccgccgag cacgaccagg cgcttcggaa cttccgtctg cgcaagcagc ccctcggagt
    10981 cgacgcagcg ctctgaatcg aggccttcga tcggggggcg aagagggaac gagccggtcg
    11041 cgatgatcgc ctgcttgaag gtgatgtcct ccccgccttc gacggcgagg gtgtgcgcgt
    11101 ccttgaacgt cccgtttccc ttgacccatt cgacgccgtt cgccttgaag agcgatgcga
    11161 cgcccgatgt catctgcttg acgaccccgg ccttccattc gttcgccgtg ccgaactcga
    11221 gctggaagcc gccgagtttg acgcccagct tgtcgaaggt gtgctcggcc tctttcagcg
    11281 caaaggctgt ctgcacccag gccttcgtcg ggatgcaccc gatccgcagg caagtgccgc
    11341 cgagctccgg ctcacgctcg acacaggcga ccctgccccc gagctgcgca gcccggattg
    11401 cggccgtgta gccacccggc ccccctccga gaacggcgac gtcgacttcc atcaagctcg
    11461 atcgtatcga ccgggcacta accaggatct gcttg
//
LOCUS       DASVEU010000174        13298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1674985,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000174 DASVEU010000000
VERSION     DASVEU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674985"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1285
                     /locus_tag="VF225_07055"
     CDS             293..1285
                     /locus_tag="VF225_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145053.1"
                     /translation="MRPLPDVPSDPALEPRLELVPEVEEAEETEADEAEETAEADEHE
                     EGDEEAKPAAKAEATQDPLKLYVRAIGDGPLLTPAEERELARRKDEGDEEAKKKLIES
                     NLRLVMSITRNYTKAGVPLLDLIQEGNLGLIRAVEKFDYKLGYKLSTYATWWIRQAVT
                     RALADQGRTIRLPVHVADQVRRLMRARRQLAQKYNREPTEKELAKESGFPEKRVRELL
                     DLVEDPVSLETPVGDGESMYADLIEDVHSERPDESTSKKLRRSELAEALLLLNPRMQR
                     VLSLRFGLNGEAPQTLEEVGAGLGITRERVRQLESRALRELRAVAPDLELYLRN"
     gene            1304..2035
                     /locus_tag="VF225_07060"
     CDS             1304..2035
                     /locus_tag="VF225_07060"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX7145054.1"
                     /translation="MERRFVDCHSHVVPSGDDGAATIQDGLALCRSAARHGTAILFAT
                     PHVWPYFTLSDEREAEIREAFERMRPHAGLELRLGFELTPAPPLLEEDPHRYLLEGTD
                     CVLMEVPFSGPPDQLYALAEHVEAAGLRPVIAHPERTEAVLEAPSIADDLAARGWMLQ
                     VNATSLTGRHGPEPEGLGWSLLERGLGRLVASDGHRTTRPAQLDEAYERAAKRLGEGA
                     LSLFDGSALGLMPSRTASRAASTGA"
     gene            complement(1989..3047)
                     /locus_tag="VF225_07065"
     CDS             complement(1989..3047)
                     /locus_tag="VF225_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145055.1"
                     /translation="MTVEIESILQHEDIQGLLENAEGTGSVKQPELAELIEAHQLDLL
                     ETDALFNELERRGIEVVVEEPEKEKEPPPPPLQTSYETTTDALQLFLREAGRHPLLTA
                     PQEVELAKRIERGDMAAKSHMIQSNLRLVVSIAKNYRNQGLPFLDLIQEGTLGLIRAV
                     EKFDWRRGYKFSTYATWWIRQAVARALADKARTIRMPVHIVERLQKMNRAERTLWTQL
                     GREPTLEEIAEEASLPIQQAHEVKAAARASASLDQPVGEAEDAVFGDFVAGDDPLPEE
                     EVEVSLRSQALTAALLSLQERERQVLVLRYGLDDSEPKTLEEIGRRLGLTRERVRQIE
                     LESLRRLSTLREMQSVTA"
     gene            3254..3610
                     /locus_tag="VF225_07070"
     CDS             3254..3610
                     /locus_tag="VF225_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145056.1"
                     /translation="MRRLFAGLAAGAMLLLTAAPAFAHDCFNPNKPEGAGVNYTITAF
                     TATGPVFEQTGPGQGIGGFVTIAPDVLGPGSPAVEVHTLGNSTAKEEVGGPGSMKPEH
                     ACDGKGIDYLGACLGG"
     gene            3683..5011
                     /locus_tag="VF225_07075"
     CDS             3683..5011
                     /locus_tag="VF225_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145057.1"
                     /translation="MHGKLRYVKLGLIGLLAALAIASPAQAELVTSQADHGLLAVAAD
                     GTPYVAYTVGRNLFMTLRTKQGRWRSARLGRLPGANVTLAGIRVSERPHRYVSVLAED
                     LRGRWLSLARGSRITSIVRPASGSSLGPAGLTLDARERPAIAYAVQRASGKSFLRLVT
                     FGEGVRPRMRAITKKGFPTSDLPPGAAPVLVNGRLHVVETYTSAAIDWAPKAGGGWEG
                     QYLFASRDGTPQGRVGAVFLPSTLWSAWTQVYPDQLVVLLTSSSETQTTWTLTHGIFV
                     SIAQGETAPEVAAYDWVSLAEDSPVYAGVVVQGPGDATWQLDGRLEGYVIAPGGARQL
                     LLSREGALEWFRAPAPSLPSIQISMGAVTETGQVSGNVAGAAGGSVEIYRELPHSPRE
                     LVAASPVAADGSFRADGLDPTPGALYRAVYVEPATGIPFGFLPGVTVGVS"
     gene            complement(5008..5949)
                     /gene="uppP"
                     /locus_tag="VF225_07080"
     CDS             complement(5008..5949)
                     /gene="uppP"
                     /locus_tag="VF225_07080"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX7145058.1"
                     /translation="MSRRQIAFTAAAGSAFLLAVVLAALRVGETPGKLSDWQALVLGI
                     VQGATELLPVSSSGHLILVPWAAGWHYLQDHPDFNKTFDVALHLGTLIAVILYFWRDV
                     VRIAVAWWQSLKRRRIETSDERIAWYIVVATVPAAIAGAAGESFIEKKLGDPWQIAIL
                     LAVFAIILWLADRVPPRRAIEGIGWRGAFGIGLAQCLALMPGTSRSGVTITAGRFLKL
                     NRDDAARLSFYLLIPIVFGAAVLKGVKVAKDGLPPGSAGPFVVGMIAAAVVGLVAIWG
                     LLGYVRRHTYTVFVLYRLALSAAILITIGAGWRSKNF"
     gene            6367..6561
                     /locus_tag="VF225_07085"
     CDS             6367..6561
                     /locus_tag="VF225_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145059.1"
                     /translation="MHLRLPAIEPGVKAFVWALLLSLYLWGFLILAVGVDKGTSTVLG
                     LIAFGAIFLFVRVNGESEEP"
     gene            complement(6564..6785)
                     /locus_tag="VF225_07090"
     CDS             complement(6564..6785)
                     /locus_tag="VF225_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145060.1"
                     /translation="MSRRLSGADNRKAMTYDSLPQGERGYELLRAALTEVEDSRPPAF
                     RRLEQAVGGELARMLVGALRPRRPRVILV"
     gene            complement(6843..7409)
                     /locus_tag="VF225_07095"
     CDS             complement(6843..7409)
                     /locus_tag="VF225_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145061.1"
                     /translation="MYGLTRATTTLVFSAAAGLLIWLATQISNDHVGGYWARVGLVAG
                     AGLLMAVSQLVGGWTKWGWPRLSIPVLVTAFIPVAIVSLWIILAGEPGSGWFHNHVMA
                     WSRDIHVSGLVTDFLYYIPVVAFGTGLVFGFSFDTTGPVVRGRDVAVDRDRKVAPVNG
                     RAADEPVSAEREATTTSTDPVTDRETVR"
     gene            complement(7546..8142)
                     /locus_tag="VF225_07100"
     CDS             complement(7546..8142)
                     /locus_tag="VF225_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145062.1"
                     /translation="MYDALTGRFTFPCPARGEAHVNLSAFRQLVRLPGAAHPSVFHVL
                     FACACGEEHDGLVTHDDLDWGPLGLQSGEFYNVMTAQLDRVVTELSDVAARRIEAGEW
                     PWSFFCYPEDRPRPVFPSSFFLLAPGDGSLGLAVRCPACQRTSVNLVSHEHVDLPFHN
                     DPKVGVVEHVFNDDVAGALEEFRAELYSASFDTRRLAL"
     gene            complement(8194..8709)
                     /locus_tag="VF225_07105"
     CDS             complement(8194..8709)
                     /locus_tag="VF225_07105"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7145063.1"
                     /translation="MNLAEKVREVPDWPQPGVGFKDVTPLLADPEALAYSISQLAEWG
                     AEKKPDLIVGAEARGFILGAAIAKEIGCGFVPARRPGKLPPETISATYALEYGQNALE
                     LHPDMVPHGARVLIHDDVLATGGTVEAIAEQLVERLGGVVVGCCFIIELGFLKGRERL
                     SKYDLHALIEY"
     gene            complement(8714..9394)
                     /locus_tag="VF225_07110"
     CDS             complement(8714..9394)
                     /locus_tag="VF225_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7145064.1"
                     /translation="MTATASRPRLLTIGTVCGMLKDEFPDISISKIRYLEDQGLLAPR
                     RTEGGYRLFSEDDVERLETILRLQRDEFLPLRVIRQELASSGARDRKRRRSGLTEQEH
                     ELELDELCERAGIDARLARELEEFGLLTPRTESGEKRYRELDAEIALACGRLAQFGIA
                     PRHLRGFRTAADREAALIEQVVGPALRARNPERRQAGLEDLESLGELAQGLGQLLFWR
                     ALRRLAST"
     gene            complement(9391..9855)
                     /locus_tag="VF225_07115"
     CDS             complement(9391..9855)
                     /locus_tag="VF225_07115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7145065.1"
                     /translation="MSHIYCQECGFQNPEAANYCARCGALLVKDEGGTESTQTFTPDE
                     GEDTSAEVLEDLGITGPALVVRSGGGRAGETFHPEGETSIGRSPDCGIFLDDVTVSRK
                     HAVIVARDGGFVVEDQGSLNGTFVNRKRVESAQLEDGDELQIGKYRLTFLNR"
     gene            complement(9863..10105)
                     /locus_tag="VF225_07120"
     CDS             complement(9863..10105)
                     /locus_tag="VF225_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145066.1"
                     /translation="MEPLPDLATLSDADLKKLIADLMQEENQVSYQRRLLHGKIDILR
                     AELVARLQKTGGASVLDQVDVDHLTEILTSRAAPPT"
     gene            complement(10206..12719)
                     /locus_tag="VF225_07125"
     CDS             complement(10206..12719)
                     /locus_tag="VF225_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7145067.1"
                     /translation="MKAVVMAGGEGNRLRPLTSNQPKPMVPVVGKPCMEHILELLREH
                     GMDDVIVTVAFLPQAIRSYFGEGETLGMQIGYSVEESPLGTAGSVRLTAKQLDETFLV
                     ISGDALCDVDLSTLVAFHKERGAAVTIGLKSVENPLEFGIVVTDEEGRVERFLEKPSW
                     GQVFSDTINTGIYVMEPEVLKHVPADRPYDFSKELFPYLLEMGRPIYGHVMDGYWQDI
                     GNLDQFRQANFDALEENVRLNIPGIRIRGNVWLGEGVEIPDLEQIEGPAYIGNYCRIA
                     PGATVGAHSVLSNSVTLRERTRTTRSVIDASTHIGRSSLIEGAVIGRSCDIRAHVRIH
                     EGVAIGDEVTIGSETVIMPGVRIYPYKEVESGSHITESLIWESRASTRLFGRDGVAGL
                     VNVDLTAEVAVRIAAALGTALKRGARVAASRESAPACRMIKRAMITGFTSTGLDVADL
                     RVLPAAVARHLLKSEGYEAGFHVGTSQSDPEVVQIKFFEQPGIQLSAAMQKEIEKNFT
                     RGELRRASFGDIGTISYPARVRESYAQDLLDGLDVEAIRARGFRLVVDYGFSAASYVL
                     PLLLGPLGVEAVSAHGFTTDRSDTGAALLREAIGQVKQLVPAVGADLGVVLDRAAERL
                     YLVDERGREVPVEQSLLLFLRLIGSNGRRGKLAFPITVTSQVDRLLEGSGLEVVRTPA
                     SLGDLTKAAAEDGVIFAGAVGGGYVFPEFLPAYDATASLCKLLELLAPVRRPLSELVA
                     DLPASTLVHRQLPCPWALKGVVMRVLTERLRDRELDLTDGIKVFDERGWAQILPDPDE
                     PLMHIYAEGRTEEDSKVLETEFRSMVEEIMQTEGAATTT"
     gene            complement(12716..>13298)
                     /gene="pgsA"
                     /locus_tag="VF225_07130"
     CDS             complement(12716..>13298)
                     /gene="pgsA"
                     /locus_tag="VF225_07130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7145068.1"
                     /translation="LGHGRILSTLMATRAVPATLQQLPNALTIARLALIPVFVALMVS
                     ADGGHSWPAGIVFGVAGVTDQIDGFLARRWHVESDFGRIFDPLADRLMIDAAVILLVV
                     QDHMPWAGLVVILGRDLLLLAGYKTIAPQGYEVNVNFLGKAATWLLYLGIGCLIVTHR
                     STEWPYWIFWTGLVLAVCAGVVYGVAAWRERKQ"
BASE COUNT         2251 a         4443 c         4430 g         2174 t
ORIGIN      
        1 gctccgcgat tccggtcagg agggcacgcg ccgcgacagt acgctcgacg ctcgagtcgt
       61 gggcgaggct atatccggcg attccgcaca aagtgtctga aaaagatgcg aagcgacctt
      121 ttggtcgctc catctttcta gacgcatcgg ctggggtcct tgttagtggg acaccggttc
      181 gaggggtctc gcgaggggct aatgatcgga atgtgtgcga acgggggcga tccctctccc
      241 gagggttgac gagcgcctca agagtccact aggccgcgcc gatacccctc gcatgcggcc
      301 gctccctgac gtgccctcgg acccggcgct cgagccgagg ctcgagctcg ttcccgaagt
      361 cgaagaggcg gaggagaccg aggccgacga ggcggaggaa accgctgagg cagacgagca
      421 cgaggagggg gacgaggagg caaagccggc ggccaaggcc gaggcgaccc aggatccgct
      481 caagctctac gtccgcgcga tcggcgacgg cccgctcctc acacccgccg aggagcgcga
      541 gctcgcccgg cggaaggacg agggtgacga agaggccaag aagaagctga tcgagtcgaa
      601 cctccggctc gtgatgtcga tcacccgcaa ctacaccaag gccggcgtcc cgctgctcga
      661 tctgatccag gaagggaacc tcgggctgat ccgcgcggtg gagaagttcg actacaagct
      721 gggctacaag ctttcgacgt acgcgacctg gtggatccgc caggcagtta cgcgcgcgct
      781 cgccgaccag ggccggacga tccgcctgcc ggtccacgtc gcggaccagg tccgacggct
      841 catgcgcgcg cgccgccaac tcgcccagaa gtacaaccgc gagccgacgg agaaggagct
      901 tgcgaaggag agcggctttc ccgaaaagcg cgtccgggag ctgctcgatc tcgtcgagga
      961 ccccgtcagc ctcgagacgc cggttggcga cggcgagagc atgtacgccg acctgatcga
     1021 ggacgttcac tcggaacgac cggacgagtc gacctcgaag aagctgcgtc gcagcgagct
     1081 cgcggaagcg ctcctcctgc tcaatccccg catgcagcgt gtgctgtcgc ttcgcttcgg
     1141 tttgaacggc gaagctccgc aaactttgga agaggtcgga gccgggctcg gtatcacgcg
     1201 tgagcgggtg cgtcaactgg agtcgcgcgc gttgcgagaa ttgcgggcgg ttgcgcccga
     1261 tctcgagctt tacctgcgga actagcaggt actacttagg gacgtggagc gtcgcttcgt
     1321 cgactgtcac tcccacgtcg tcccgtccgg tgacgacggg gctgccacca tccaggacgg
     1381 actcgccttg tgcaggagcg ctgcgcgaca cggcacggcg atcctcttcg cgacgccgca
     1441 cgtctggccg tacttcaccc tgagcgacga gcgcgaggcg gagatccgcg aggccttcga
     1501 gcgcatgcgt ccgcacgcgg ggctcgagct caggctcggg ttcgagttga cgccggcacc
     1561 accgctcctg gaggaggacc cgcaccgcta cctcctcgaa gggaccgact gtgtcttgat
     1621 ggaggtgccg ttctccggtc cgcccgatca gctctatgca ctcgcggagc acgtggaagc
     1681 cgcgggcctg cggcccgtga tcgcgcatcc cgagcggacg gaggccgtgc tggaggcccc
     1741 gtccatcgcg gacgatctcg ctgcacgcgg ctggatgttg caggtgaacg cgacgtcgct
     1801 gaccggccgc cacgggcctg agcccgaggg gctgggctgg tcgctgctcg aacgtggcct
     1861 cggcaggctc gtcgcctcgg acggacaccg tacgacacgc ccggcgcagc tcgacgaggc
     1921 gtatgagcgc gctgcgaaac gcctgggaga aggcgcgctc tctttgttcg acgggtccgc
     1981 gctcggtctt atgccgtcac ggactgcatc tcgcgcagcg tcgacaggcg cctgagcgac
     2041 tcgagctcga tctgcctcac tcgctcgcgg gtgagaccca gacgccggcc gatttcctct
     2101 agcgtcttcg gctcggagtc gtcgaggccg tagcgcagga cgagcacctg gcgctcgcgc
     2161 tcctgcagcg acagcagcgc agcggtcagc gcctgagagc gaagcgagac ctcgacctct
     2221 tcctcgggaa gcgggtcgtc gccggcgacg aagtcgccga agacagcgtc ctcggcctcg
     2281 ccgaccggct ggtccagaga tgcggaggca cgtgctgcag ccttgacctc gtgggcctgc
     2341 tgaatcggca ggctcgcctc ctcggcaatc tcctcgagtg tcggctcgcg gccgagctgc
     2401 gtccagagcg tccgctccgc gcggttcatc ttctgcaggc gctcgacgat gtgcacgggc
     2461 atgcggatcg tccgcgcctt gtcggcaagc gcacgcgcaa ccgcttgccg gatccaccag
     2521 gtcgcgtagg tcgagaactt gtagccacgg cgccagtcga acttctcgac cgcgcggatg
     2581 aggccgagcg ttccttcctg gatcagatcg aggaagggga gcccctggtt gcggtagttc
     2641 ttcgcgatcg acacgacgag ccggaggttc gactgaatca tgtgggactt cgcggccatg
     2701 tctccgcgtt cgatacgctt cgcgagctcg acctcctggg gcgcggtcag cagcggatgg
     2761 cggccggcct cgcgcaggaa gagttgcaac gcatcggtcg tcgtctcgta cgaggtctgg
     2821 agcgggggag ggggcggctc tttctccttc tccggctcct cgacgacgac ttcgatcccc
     2881 cgccgctcga gctcgttgaa gagcgcgtcg gtctcgagca ggtcgagctg atgagcttcg
     2941 atcagctcag caagctcggg ctgcttgacc gagcccgtgc cttccgcgtt ctccagcaaa
     3001 ccttggatgt cctcgtgctg gaggatcgac tcgatctcga cggtcatctc cctgggtgat
     3061 tgttgcctct ggccccgcag tcccaaacac gcacgctctc acggcccgac gggttcagtc
     3121 aaccctctgg gcgcctttcc gcgcgaacag gcggcccagg gcggatctct gccatgaggt
     3181 tcagccgtga gcccaccgta gctgtgaaag gattgggccg gtaactgtcg tctatctggg
     3241 gagaatgaaa gtgatgcgaa ggctctttgc gggtcttgct gctggagcga tgttgctgct
     3301 caccgcggcg cctgcgtttg cgcacgactg cttcaacccg aataagcccg agggtgcggg
     3361 cgtcaactac acgatcacgg cttttacagc gaccggcccc gtgttcgaac agaccggccc
     3421 cggccagggg atcggcggtt tcgtgacgat cgcacctgat gttctcggtc ccggttcacc
     3481 ggcggtcgag gtgcacacac tcggaaacag cactgccaaa gaagaagtcg gcggtccggg
     3541 aagcatgaag ccggaacacg cctgcgacgg caaaggcatc gattacctcg gcgcctgcct
     3601 cggcggctaa gagcgcctcg gattctcgtt ccaggagccg gctcggcctc gagctgggcc
     3661 ggctcatcta gcctcgatcc tgatgcacgg aaagctgcgt tatgtcaagt tggggttgat
     3721 cgggctgctg gcggccctcg cgatcgcctc tcctgcccag gcagagctcg tgacgagcca
     3781 ggccgaccac ggcctgctcg cagtcgcagc ggacgggacg ccgtacgtgg catacacggt
     3841 gggccggaat ctgtttatga cgctgcggac caaacagggt cgttggcggt cggctcggct
     3901 cgggcgcctt cctggagcga atgtgacgct cgccgggatc agggtcagcg agcgtccgca
     3961 ccggtacgta agcgtgctcg ccgaggatct gcggggtcgc tggctctccc tcgcccgcgg
     4021 cagccggatc acgtcgatcg tgcggcccgc ctccgggagc agcctcgggc ctgccggctt
     4081 gaccctcgac gcccgcgagc gtcccgcgat cgcgtacgcg gttcagcgtg cttccgggaa
     4141 gagctttctc cgattagtga cgttcggcga aggggttcgg cctcgcatgc gcgcgatcac
     4201 gaagaagggc tttccgacga gcgacctccc gcccggggcc gcgcccgtgc tcgtgaacgg
     4261 gcgcctccac gtcgtcgaga cctacacctc cgcagccatc gactgggccc cgaaggccgg
     4321 cggcggctgg gagggccagt acctcttcgc gagccgggac gggacgcctc agggccgcgt
     4381 gggcgccgtc ttcctcccct ccaccctctg gtcggcgtgg acgcaggtgt atcccgacca
     4441 gctcgtggtc ctcctgacgt ccagctcgga gacgcagacg acgtggacgc tgacgcacgg
     4501 gatcttcgtc tcgatcgcgc agggcgagac cgcgcccgag gtcgccgcct acgactgggt
     4561 gagcctcgca gaggattcgc cggtctatgc gggggtcgtg gtgcaagggc ccggtgacgc
     4621 gacgtggcag ctcgacggac ggctcgaggg gtacgtgatc gcgcccggcg gcgccaggca
     4681 gctcctgctc tcccgtgagg gcgcgctcga gtggtttcgc gcgccggcgc cttcgctccc
     4741 ttccatccag atcagcatgg gcgcggtcac cgagaccggt caggtcagcg gcaatgtcgc
     4801 cggtgcggcc ggcgggagcg tcgagatcta ccgggagctg ccccactctc cgcgcgagct
     4861 ggtcgcggcg agcccggttg ccgccgacgg ctccttccgg gccgacggcc tcgacccgac
     4921 tccaggcgcc ctctaccggg cggtgtacgt cgaaccggcg accggaatcc ccttcgggtt
     4981 cctcccgggc gtgacggtcg gcgtttccta gaagttcttg ctgcgccagc cggcgccgat
     5041 cgtgatcagg atggccgccg agagcgccag ccggtagaga acgaaaacgg tgtacgtatg
     5101 ccggcgcacg tagccgagga ggccccagat cgcgacgagc ccgaccaccg ccgccgcgat
     5161 catgccgacg acgaacggcc cggccgatcc gggcggcagg ccgtccttgg ccacctttac
     5221 gcctttcaag acggccgcgc cgaagacgat cgggatcagg agatagaacg acaggcgagc
     5281 cgcatcgtct cggttcagct tcaggaaacg cccggcggtg atcgtgacgc cggacctcga
     5341 cgtcccgggc atcagcgcga gacactgcgc caggccgatg ccgaacgctc cgcgccagcc
     5401 gatgccctcg atcgcgcgcc gcggcggaac gcggtcggca agccagagga tgatcgcgaa
     5461 gaccgcgagc aggatcgcga tctgccaggg gtcaccgagc ttcttctcga tgaagctctc
     5521 ccccgccgcc ccggcgatgg cagccgggac ggtcgcgacg acgatgtacc aggcgattcg
     5581 ctcgtccgat gtctcgattc gccgccgctt gaggctctgc caccaggcga cggcaatgcg
     5641 cacgacatcg cgccagaagt agaggatcac cgcgatcaac gtccccaggt gaagcgctac
     5701 gtcgaaggtc ttgttgaagt ccggatggtc ctggaggtag tgccagccgg ccgcccacgg
     5761 gacgaggatg aggtgtcccg aggacgagac cggcagcagt tcggtcgcac cctggacgat
     5821 gccgaggacc agggcctgcc agtcggagag cttcccgggc gtctccccta cgcgcaatgc
     5881 ggcgagcacg acggcgagaa gaaacgcaga gcccgcggcg gccgtgaatg cgatctgccg
     5941 gcggctcacg ggcaagatta tgtcggatgc gttagtgtcg gcgtgcgttc gaccgacgca
     6001 tgtgcggcgt cacttcgaga atcttccggt cggcgccgat cctttccaca caggtatcaa
     6061 cacgttggaa gattggctgt ggaaatgttg gaaaaggaaa acttcgcaaa ttgcggccac
     6121 gacctttttc gaccccttgc atcatttgct tgacacgtta gattgcccat tgcgccgagc
     6181 acagagcgcg agtaggtcga aaccgagtcc gaaggcagta gccggaaggc gaaagtcagc
     6241 ggaagcggtg gacgaaagtg aaactggctc ggcgaatttt tttggtccaa aggcgtcgac
     6301 tcggcgccgc cgcactggag gccgtatgag cttcgctcat gcggccgttt ctttagggtg
     6361 cctgcggtgc acctgcgact cccagctatc gaaccaggcg tcaaggcttt cgtctgggcg
     6421 ctcctcctga gcctctacct gtggggcttc ctcatcctcg cggtcggggt cgacaagggc
     6481 acgtcgacag tgctgggact gatcgctttc ggcgcgatct tcctcttcgt ccgcgtcaac
     6541 ggcgagagcg aagagcccta gccctagacg aggattacgc gcgggcggcg tggcctgagc
     6601 gcgcccacca gcatccgagc gagctcccct ccgaccgcct gctccagacg gcggaaggcg
     6661 ggaggtcggg agtcctcgac ctcggtcagg gcagcgcgga gcagctcgta gcctctctcg
     6721 ccctgcggta gcgagtcgta cgtcatggct ttccggttat cggcgccgga cagccgacgg
     6781 ctcatccctg agccaaggct aaagccgccc cccgggcttg ccgaggggcg gcctcaaccg
     6841 agttatctga ccgtctcgcg gtccgtcaca ggatcggtcg acgtcgtcgt agcctctcgc
     6901 tcggcggaca ccggctcgtc ggccgcccgc ccgttgaccg gtgcgacctt gcggtcgcgg
     6961 tcgacggcca cgtcgcggcc acgtacaacc ggaccggtcg tgtcgaacga gaacccgaag
     7021 acgagccccg tcccgaacgc caccaccggg atgtagtaca ggaagtccgt cacgagaccg
     7081 ctgacgtgga tgtcccggct ccaggccatc acgtggttgt ggaaccaacc gctgcccggc
     7141 tcgccggcca ggatgatcca caacgacacg atcgccaccg gaatgaacgc ggtgacgagg
     7201 accgggatcg agagccgcgg ccagccccac ttcgtccagc ctccaaccag ttgggacact
     7261 gccatcagga gacccgcgcc ggcaacgagc ccgactcgag cccagtagcc cccgacatgg
     7321 tcgttgctga tctgcgttgc gagccagatc agcagaccgg cggcagccga gaacaccagt
     7381 gtcgtcgtcg cacgtgtgag tccgtacatt cgatcacctc gctagtcggt gcctcggtga
     7441 tacccggccc cgctgaagac gaatccgtaa acccgagaat gcgcgagtac aagccgtcgc
     7501 cacaggatcc ggcctccatg cggcgtcgcc gagtcgacgc cttggctaga gcgccaggcg
     7561 gcgagtatcg aacgaggcgg agtagagctc ggcgcggaat tcctccagag cgccggcgac
     7621 atcatcgttg aagacatgct cgaccactcc caccttgggg tcgttgtgga agggcaggtc
     7681 gacgtgctca tgggacacga ggttcaccga cgtgcgctgg caggcagggc agcgcacggc
     7741 gagcccgagg gagccgtccc ccggcgcaag caggaagaac gaggacggaa agacgggccg
     7801 cggccgatcc tccgggtaac agaagaagct ccacggccat tcccccgcct cgatcctgcg
     7861 cgccgcgacg tcggagagct cggtgacaac gcgatcgagc tgtgcggtca tgacgttgta
     7921 gaactcgccg ctctgcaggc cgagcggtcc ccagtccagg tcgtcgtggg tgacgagccc
     7981 gtcgtgctcc tccccgcatg cacaggcgaa gagcacgtgg aagaccgagg gatgagcagc
     8041 gccgggcagg cgcacgagct gacggaaggc cgaaaggttg acgtgcgcct ctccccgtgc
     8101 ggggcacggg aaggtgaagc gccccgtcag cgcgtcgtac atggtgcctt tctaatcggc
     8161 aggccggatg tcaaacctga accctggtaa caactagtac tcgatcaggg catgcaggtc
     8221 gtacttggac aggcgctcgc ggcctttcag gaaccccagc tcgatgatga agcagcaccc
     8281 gacgacgacg ccgcccaggc gttccaccag ctgctccgcg atcgcctcga cggtaccccc
     8341 cgtagcgagc acgtcgtcgt ggatcaggac gcgcgccccg tgcggaacca tgtccggatg
     8401 cagctcgagg gcgttctgtc cgtactcgag cgcgtatgtg gcgctgatcg tctcgggcgg
     8461 gagcttgccc ggccgccgcg cgggcacgaa cccgcagccg atctccttgg cgatcgcggc
     8521 tccgaggatg aacccacgtg cctcggcgcc gacgatcagg tcgggcttct tctccgcgcc
     8581 ccactccgcg agctgcgaga tcgaataggc gagcgcctcg ggatccgcga gcagcggcgt
     8641 gacatccttg aaaccgacac cgggctgcgg ccagtcgggg acctcccgga ccttctctgc
     8701 gagattcacc gggttatgtt gacgcaagcc tgcgcagcgc gcgccagaag agcaactggc
     8761 cgaggccttg cgcgagctcg ccgagggatt cgaggtcctc cagaccggcc tgccgccgct
     8821 ctggattgcg ggcacgaagg gccgggccga cgacctgctc gatgagcgct gcctcgcgat
     8881 cggccgccgt gcggaatccc cggagatgcc gaggagcgat tccgaactgc gccagtcgac
     8941 cgcaggcgag ggcgatctcg gcgtccagct cgcggtagcg cttctcgccg gactcggtgc
     9001 gcggtgtcag caatccgaac tcctcgagct cgcgcgcgag ccttgcgtcg atcccggcgc
     9061 gctcacatag ctcgtcgagt tcgagctcgt gctcctgctc cgtcaacccg ctgcgccgcc
     9121 gtttgcggtc acgcgcgccg gaagaggcaa gctcctggcg gatcacgcgc agcggcagga
     9181 actcgtcgcg ctgcaggcgc aggatcgtct cgaggcgctc gacgtcgtcc tcgctgaaga
     9241 ggcgataccc gccctccgtc cgccgcggcg cgagcagccc ctggtcctcg aggtagcgaa
     9301 tcttcgagat cgagatgtcc gggaactcgt ccttgagcat cccgcagacg gtcccgatgg
     9361 tcagtagacg cggtctcgag gcggttgccg tcacctgttg agaaaggtca gtcgatattt
     9421 gccgatctgc agctcgtcgc cgtcctcgag ctgtgcggac tcgacacgct tccggttcac
     9481 gaacgtgccg ttgaggctgc cctggtcctc cacgacgaag ccgccgtcgc gggccacgat
     9541 cacggcgtgc ttgcgcgaga ccgtgacgtc gtcgaggaag atcccgcagt ccggtgaacg
     9601 gccgatcgac gtctcgcctt ccgggtggaa cgtctccccc gccctgccgc cacccgagcg
     9661 gacgaccagc gcgggaccag tgatcccgag gtcctcgagc acttcggcgg acgtgtcctc
     9721 gccctcgtcc ggcgtgaacg tctgcgtcga ctccgtgccg ccctcgtcct tgacgagcag
     9781 agcgccgcag cgcgcgcagt agttcgcagc ttccggattc tggaagccgc attcctggca
     9841 gtagatgtgg ctcactgggc ccctaggtgg gcggagcggc tctcgaggtc aggatctcgg
     9901 tcaggtgatc gacgtccacc tggtcgagga cgctcgcccc tcccgtcttc tgcagccgcg
     9961 caacgagctc cgcacgaagg atgtcgatct tcccgtgcag cagccgccgc tggtaggaga
    10021 cctgattctc ctcctgcatc agatcggcga tgagcttttt gagatcggcg tcggacagcg
    10081 ttgcgagatc gggcagaggt tccacgggcc gcctttcctg gtcgagggct cgtcagcagt
    10141 aaagcagcga acctgcaaag ggtcaacctc agcttgagag tttacccact gcctgcccag
    10201 tccgcctaag tcgtagttgc cgcgccctcc gtctgcatga tctcctcgac catcgaccga
    10261 aactcggtct cgaggacctt cgagtcctcc tcggttcgcc cctcggcata gatgtgcatg
    10321 agcggctcgt ccgggtcggg aaggatctgg gcccagccgc gctcgtcgaa gaccttgatg
    10381 ccgtccgtca gatcgagctc gcggtcacgc aggcgctcgg tcaacacccg catcacgacg
    10441 cccttgaggg cccagggaca cggaagctgc cggtggacca gggtcgaagc aggcagatcg
    10501 gcgacgagct ccgacagagg cctccgcaca ggcgcgagga gctcgagcag cttgcagagg
    10561 ctggcggtcg cgtcatacgc aggcaggaac tccggaaaga cgtaaccgcc acccaccgcc
    10621 ccggcgaaga tgactccgtc ttcggcggcc gccttcgtga ggtcgccgag cgacgcgggc
    10681 gtgcgcacga cctcgagacc gctcccctcc aacagccgat ccacctggct cgtgacagtg
    10741 atcgggaacg cgagctttcc ccgccggccg ttgcttccga tcagccgcag gaagaggagc
    10801 aggctctgct cgaccggaac ctcgcggccg cgctcgtcga ccagatagag gcgctcggcc
    10861 gcacggtcga ggaccacgcc gagatcggcg ccgaccgcgg ggacgagctg ctttacctgc
    10921 ccgatcgcct ctcgcagcag agccgcgccg gtgtcggacc ggtccgtcgt gaagccgtgc
    10981 gcggagacag cctcgacccc gagcggtccg aggagcaacg gcagcacgta tgacgcggcc
    11041 gagaagccgt agtcgacgac gaggcgaaag ccgcgcgcac ggatcgcttc cacgtcgaga
    11101 ccgtcgagca gatcctgcgc gtacgactcg cgtacgcgtg cgggatagct gatcgtgccg
    11161 atgtcgccga agctcgcgcg gcgcaattcc ccgcgcgtga agttcttctc gatctccttc
    11221 tgcatcgccg ccgagagttg gatgcccggc tgctcgaaga acttgatctg caccacctcg
    11281 gggtccgact ggctcgtccc gacgtggaag cccgcctcgt agccctcgct cttgaggagg
    11341 tggcgcgcga ccgccgcggg cagaacgcgt agatcggcga cgtccagccc cgtggacgtg
    11401 aagccggtga tcatcgcccg cttgatcatg cggcacgcgg gcgcggactc gcggctggcg
    11461 gcaacgcgcg cgccgcgctt cagcgccgtc ccgagtgcgg ccgcgatgcg cacggcgacc
    11521 tcggcagtga ggtcgacgtt gacaaggcct gcgacgccgt ccctgccgaa gagccgcgtc
    11581 gacgcgcggg attcccagat caggctctcg gtgatgtgcg agccggactc gacctccttg
    11641 tacgggtaga tccgaacgcc cggcatgatc accgtctccg agccgatcgt cacctcgtcg
    11701 cctatggcga ctccctcgtg gatccggaca tgcgcacgga tgtcgcaaga gcggccgatc
    11761 acggcgcctt cgatcaacga gctgcgaccg atgtgcgtcg acgcgtcgat gacggaacga
    11821 gtcgtccgtg ttcgctcgcg cagcgtgacg ctgttggaga ggaccgagtg cgcgccgacc
    11881 gtcgcgccgg gagcaatgcg gcagtagttg ccgatgtacg ccggcccttc gatctgctcg
    11941 aggtcgggga tctcgacccc ctcgccgagc cagacgttgc cgcgaatgcg gatgcccgga
    12001 atgttgaggc gcacgttctc ctcgagggcg tcgaagttcg cctgccggaa ctggtcgaga
    12061 ttcccgatgt cctgccagta gccgtccatg acgtgcccgt agatcgggcg gcccatctcc
    12121 agcagatacg gaaagagctc tttcgagaag tcgtacggtc tgtcggccgg cacgtgcttg
    12181 agcacttcgg gctccatcac gtagatgccc gtattgatcg tgtccgagaa gacctggccc
    12241 caggacggct tctcgagaaa gcgttccacc cgtccttcct cgtccgtgac gacgatgccg
    12301 aactcgagcg ggttctcgac tgatttcagc ccgatcgtta ccgccgcgcc tctttccttg
    12361 tgaaaggcga cgagcgtgct caggtccacg tcgcaaaggg cgtcgcccga gatcacgagg
    12421 aaagtctcgt cgagctgctt cgcggtcagg cggaccgagc cggccgtacc gagtggcgac
    12481 tcctcgaccg agtaaccgat ctgcattccc agcgtctccc cttcgccgaa gtaactgcga
    12541 atcgcctgcg gcaggaaggc gaccgtgacg atcacgtcgt ccattccgtg ctcgcgcagc
    12601 agttcgagaa tgtgctccat gcacggcttg ccgacgaccg gcaccatcgg cttcggctga
    12661 ttggacgtga gggggcgcag gcggttgccc tcgccacccg ccatcacgac cgccttcatt
    12721 gctttctctc tctccaggcc gcgaccccgt acaccacgcc tgcgcagacc gcgaggacga
    12781 gacccgtcca gaagatccag tagggccact cggtcgaacg gtgcgtgacg atcaggcatc
    12841 cgatcccgag atagaggagc caggtcgccg ccttgccgag gaagttcacg ttcacctcat
    12901 agccctgggg cgcgatcgtc ttgtagccgg cgaggagcag gaggtcacgc ccgaggatca
    12961 cgaccaggcc ggcccacggc atgtggtcct gaacgacgag gaggatcacc gccgcgtcga
    13021 tcatcagccg gtcggccagc gggtcgaaga tgcgcccgaa gtcggattcc acgtgccatc
    13081 gccgcgcgag gaagccgtcg atctggtcgg tgacgccggc aacgccgaag acgatcccgg
    13141 ccggccagct gtgcccgccg tccgcgctga ccatgagtgc gacgaagacc gggatcagag
    13201 ccagccgcgc gatggtcagc gcgtttggaa gctgctgcaa ggtcgccgga acggcgcggg
    13261 tcgccatgag tgtggaaagg atacgcccgt ggccgaga
//
LOCUS       DASVEU010000175         8281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_704332,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000175 DASVEU010000000
VERSION     DASVEU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704332"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VF225_07135"
     CDS             <1..565
                     /locus_tag="VF225_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7145069.1"
                     /translation="IGRYAAAERLPLHVHADEQPREIEECLAEHGIRPIELLDHTGCL
                     TERTTVVHATHADGVEFDLVARAGARICACPTTEANLGDGFLPVERVCARGIGICIGS
                     DSNVRVDPFEELRELEGIARRQTGRRNVVSTETLLCFGADEGAGSLGLEEWGDVEVDL
                     SHPSLRGVEDVFDALVSGCGADVLLKA"
     gene            complement(551..1504)
                     /locus_tag="VF225_07140"
     CDS             complement(551..1504)
                     /locus_tag="VF225_07140"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145070.1"
                     /translation="MRRLLLALVLLLAMPASAGATQALPRSAIFFYPWYSTPPQDGQY
                     VHWTQGGHTPPFDIGSAFFPMRGTYSSGDARVLAAQMRDIAAAGVDEVVSSWWGRGSP
                     EDGRLQAVLRAAKRRGLQVAVQLEPYEGRTVESIASDLVYLRGLGLRDVYVYNAKDFT
                     VEDWRRVTLQPTGLRLFAQTNHVGFAARAGFMGFYTYDILLYDAAKFDRFCTQARALG
                     ILCAPSVGPGYDAGEATGDFRVKPRLDGATYDSMWGAALRAGADMVTITSYNEWSEGT
                     QIEPAGHGGRYESYDGTYGLHGRAAQRAYIMRTAYWTSRFQ"
     gene            complement(1899..2357)
                     /locus_tag="VF225_07145"
     CDS             complement(1899..2357)
                     /locus_tag="VF225_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145071.1"
                     /translation="MKKWKRYRSLRRLVVAFAAVAVAVPIAQAKAQAAQNDPGSTKPA
                     TAQTAKRVYGISVDSYRRLPAEDQEALRPPRSAAIGSHSPSSAVGGYSPPAPAKTRLV
                     VTSTPVAYDTSDTFSWGGAGIWVASGLVLMSFAALAVLLVPRSRMGQPAV"
     gene            complement(2886..3188)
                     /locus_tag="VF225_07150"
     CDS             complement(2886..3188)
                     /locus_tag="VF225_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145072.1"
                     /translation="MARPLLTALAPILGATLTGGGTRGTAATPTPPGGGTIPAPSSWT
                     TPGYLTNLPATPSLGPPGPTLTANPQAGQAALLAALVGRGLRFNPSVGGSNYVKAY"
     gene            complement(3340..3540)
                     /locus_tag="VF225_07155"
     CDS             complement(3340..3540)
                     /locus_tag="VF225_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145073.1"
                     /translation="MKKYLQKLRFWPSGGKPGPAAAMPEQSPNPQPTPEQNAEQLRRE
                     ALLKDDRKVDERDDSPRPDYDP"
     gene            complement(3544..3618)
                     /locus_tag="VF225_07160"
     tRNA            complement(3544..3618)
                     /locus_tag="VF225_07160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3581..3583),aa:Pro,seq:ggg)
     gene            complement(3639..3938)
                     /locus_tag="VF225_07165"
     CDS             complement(3639..3938)
                     /locus_tag="VF225_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7145074.1"
                     /translation="MARKRRFSEDSFGPTIERLMAEAGLTYRSLAEKTRLSAGYLNHL
                     VHGNRPVPSDDVIKTLARALGVEAEHFREYRLRVITDRLERMPDLIDRLYKRYGA"
     gene            complement(3938..5611)
                     /locus_tag="VF225_07170"
     CDS             complement(3938..5611)
                     /locus_tag="VF225_07170"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7145075.1"
                     /translation="MIARASQLYLPTLREPPADAEAASHRLLVRGGFIRQVGAGLWTF
                     LPLGWRVHQKVVQIIREEINAIGGQEMLEPVLTPAELWETSGRIRIPIVFKLKDRAGR
                     DFVLPFSHEETMTFHARELQSYKQLPQLWYHFSTKERDEPRPRGGLLRVREFIMKDSY
                     SFDRDEAGLDKSFDAHKAAYERIWQRCGLEAYYVEAESGIMGGRESSGFMAPAASGEN
                     VLVRCENGDYYADYDAARGIPRAPEFPEPLEQPEEVETPGVTTIEALAEFLGIDPAAT
                     SKAMPVMIGERPVLALIRGDDRLSEEKLVTTLGEAYRPMTGEEIRAVFGAGGGSLGPV
                     GVTIDIVADEALREGQFVGGANRDGWHLRGVQAGRDYTPSFADIRQANQGDACVVCGG
                     RLIEQPAIELGHIFKLGTFYSVRFGATFLDEDGEEKPLVMGSYGIGPGRTMAAIVEQH
                     HDERGIQWPASVAPYDVHVVVIPGMEEHAQPVADALSTAGFDVLVDDRDARAGEKFAD
                     ADLIGCPVRVTVGRKAAEDGTVDIRERAKGEDKAVSVGELVSTVREKLG"
     gene            complement(5626..6762)
                     /gene="ispG"
                     /locus_tag="VF225_07175"
                     /gene_synonym="gcpE"
     CDS             complement(5626..6762)
                     /gene="ispG"
                     /locus_tag="VF225_07175"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7145076.1"
                     /translation="MASERQISVGGVKIGGGAPVVVQSMTLTKTHDVEATTAQIAALA
                     SAGCEIVRVAVPKTEDAEALGRIIRLSPLPVIADIHFNASLALKAIDAGVAAVRINPG
                     NIGGSDKVAQVVRAAQAAKIPMRIGANSGSLPKHLNELAQKDQAEALVEAALEEVRLL
                     ESLDFREFKISVKSSHVPTMIRAYRQLASKVPYPLHLGVTEAGTPFSGSIKSAVGMGA
                     LLVDGVGDTMRVSLTADPVEEVRVAFEILKSLGLRERGPIMIACPSCGRDNVGVQTLA
                     EKVEERLHAYPQHFEVAVLGCAVNGPGEAGDADFGIAGGRDTGFVYAHGRVLKKVSSD
                     ILIDELFHEIDKWIADGMERPKRLKMAKPAALAMAEASLIPLSD"
     gene            complement(6922..8148)
                     /gene="rseP"
                     /locus_tag="VF225_07180"
     CDS             complement(6922..8148)
                     /gene="rseP"
                     /locus_tag="VF225_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7145077.1"
                     /translation="MSIFVAILGLALLILVHEAGHFFVARAVGMRPRRFYLGFPPALV
                     KVVRNGIEYGIGAIPLGGYVKIPGMHRPAASDLDVQLAPALAEDPSLFPKVARVKRGL
                     EANDLDSARDALPELERAVEEATLSPSAGKAARRSVEEVQDGLSSDAYWRQRTWKRVA
                     VIFAGPGVNIVVAIVLVALGYLLGVPGDPDRTVKNVKANTPAASVGLHAGDTIIAVNG
                     RETPNFDTVSRLIRASKGKPITVTVLRNGKAVMLGPVRTKQIGSRWALGFQPGWKEVS
                     YGPVKALTKSLSITWETTKATITFLPRLATGAGRKQVSSPVGIVDVSSQAVSLTFTLY
                     LQILGLISISLAILNLLPLLPLDGGHILFSIIEGLRGRAVGREVYERVSAIGIALFLV
                     LTFIGLSNDINHLGGG"
BASE COUNT         1366 a         2782 c         2711 g         1422 t
ORIGIN      
        1 gatcgggcgc tacgcggccg ctgaacgtct gccgctgcac gtgcacgcgg acgaacagcc
       61 gcgcgagatc gaggagtgcc tcgccgagca tgggatccgg ccgatcgagc tcttggatca
      121 caccggctgc ctcaccgagc ggacgacggt cgtccatgca acccacgccg acggcgtcga
      181 gttcgatctg gttgcccgcg ccggtgcgcg catctgtgcc tgtccaacaa ccgaggcgaa
      241 tctgggtgac ggctttctgc cggtcgagcg cgtctgcgct cgcgggatcg ggatctgcat
      301 cggctccgac tccaatgttc gcgtcgaccc attcgaggag ctgcgcgagc tcgagggcat
      361 cgcccgacgt cagacggggc gtcgcaacgt cgtctccacc gagacgctgc tctgcttcgg
      421 cgccgacgaa ggtgcgggct cactcggcct cgaggagtgg ggcgacgtcg aagtcgatct
      481 ctcgcaccca tcgttgcgcg gtgtcgagga cgtcttcgac gcactcgtca gcgggtgcgg
      541 cgccgacgtc ttattgaaag cgtgacgtcc agtacgccgt gcgcatgatg taggcgcgtt
      601 gcgcggcccg accgtgcagc ccatacgttc cgtcgtagct ctcgtaccgg ccaccgtggc
      661 ctgcgggctc gatctgcgtc ccttcgctcc actcgttgta ggacgtgatc gtcaccatgt
      721 ccgcgccggc gcgcagcgcg gcgccccaca tcgagtcgta cgttgcgccg tcgagcctcg
      781 gtttcacacg gaagtctccg gtcgcctcgc cggcgtcgta gcccggaccc accgagggtg
      841 cgcagagaat gccgagggcg cgcgcctgcg tgcagaagcg gtcgaacttc gctgcgtcgt
      901 agagaaggat gtcgtaggtg tagaagccca tgaagcctgc gcgggccgcg aagccgacgt
      961 gattcgtctg ggcgaacagg cgcagtcccg ttggctggag tgtcactcgg cgccagtctt
     1021 cgacggtgaa gtccttggcg ttgtagacgt agacgtcgcg taggccgagg ccccgcaggt
     1081 acacgagatc ggaagcgatc gactccaccg tccggccctc gtacggctcg agctgtacag
     1141 ccacttgaag gccgcgccgc ttggccgcgc gcaacaccgc ctgcaggcgt ccgtcttctg
     1201 gcgaccccct cccccaccac gagctgacga cctcatcgac gccggctgcc gcgatgtccc
     1261 tcatctgagc cgcaagcacg cgcgcatcgc cgctcgagta ggtgccgcgc atcggaaaga
     1321 aggccgaccc gatgtcaaaa ggcggggtgt gcccgccctg cgtccagtgg acgtactggc
     1381 cgtcttgggg cggagtggag taccaggggt agaagaagat cgcgctccga ggcagcgcct
     1441 gggtggcgcc agcggatgcc ggcatggcca ggagaagcac caaggccagg aggagacgcc
     1501 gcatcccgca tagcttctca atcaggccgg atacccgaat ccccaggacg gggcataagg
     1561 tgggacgggg cccgcgccga tgccacggat atgccgcgcc ctggttggtt cgaccgcatt
     1621 ctttcccgct gccgacgcga gaccgctcag acgatggcgg agtacgcggt ggtcctcgcc
     1681 gtcatcactc cggggatcat catcgtcctc gggttgttct cggacgacat cggccgcttg
     1741 ctcgacagcg tccgcgcgct cttctagacg cgcgaaacgc catacctaca agtcgagatc
     1801 gtcgggggca agctcgtact gcgaccgtcg cgcgagctcc ctcgacacct ctgacacgct
     1861 ccctagcttg attgcgtgga agtcacggac tggacggttc agaccgccgg ctggcccatg
     1921 cggctccggg gaaccaacag gacggccagt gcggcgaacg acatcagcac gaggccgctc
     1981 gcgacccaga tgcccgcgcc gccccagctg aacgtgtcgg acgtgtcata cgcgaccggc
     2041 gtcgaagtga cgacgaggcg ggtcttggcg ggtgccggcg gagagtagcc tccgaccgcg
     2101 ctcgagggag agtggcttcc aatggcggcg ctgcgcggag gtcgaagcgc ttcctggtcc
     2161 tcggccggca gccgccggta cgagtcgacg gagattccgt agacgcgctt cgcggtctgc
     2221 gccgtcgctg gttttgtgga acctgggtcg ttctgggccg cctgcgcttt cgcctgcgcg
     2281 atggggaccg caacggccac ggccgcgaac gccacgacca gccgtcggag agaccgatat
     2341 cgcttccact tcttcatgtc ctccgctccc tttcctcggt tccatctgcc ctgacgaccc
     2401 cacgatcgcg ctcggcagta acgaaaccgc cacgggccac ccaccgccgc ccaccgcggg
     2461 gtaacgaacc ggtcgtgaga gggtgtgagc aggaggtcga cgagaacggc aaccggcgag
     2521 atgtggattg aacgaaagga cgaaacacat gactgagaag aaggtttggt tcacggctga
     2581 agcacgaggt aggtgacgat gcgggcaacc gtcatgtacg gcgccggtga cgttcacgtc
     2641 gagaacgttc ccgatcccgc cgtgatcgag ccgaccgacg cggtcgtgcc acgaggcgat
     2701 tggtgtcgtc gaggccgtcg gcgccgacgt acgcaccctg aagatcggtg acgtggtcgt
     2761 aataccgttc gcgttctccg acggcacctg cgtcttcaac tgactgcgat ccgcgagcag
     2821 caagccaagc ggcttagatc gcgatcttca tgtcgacgtg gcgcccttcg gcgcgctccg
     2881 tcgttctagt acgccttgac gtagttcgag ccgccgacac tcgggttgaa cctcaggccc
     2941 ctaccgacga gcgcagccaa caaagcggcc tgccctgcct gcgggttcgc ggtgagcgtc
     3001 ggccccggcg gacccaaaga cggtgtcgcc ggcaggttcg tcaggtagcc cggtgtcgtc
     3061 cacgacgacg gcgcgggaat cgtccccccg cctgggggtg tcggtgttgc tgccgttcca
     3121 cgcgtcccac cacccgtcag tgtcgctccc agaataggtg ccagggctgt gaggagcggc
     3181 cttgccaccg gtcgcgcctc gagcgaggta agcacgttcg acatcgcaaa gatcgcggga
     3241 ccgtcaaccg agcagatcga gaagacgtac gcgcatctgt tgaagggcca cgccgacagg
     3301 tggcgcacag ctctcgacaa gtacgacgcg cagaagccgt caggggtcgt agtcgggacg
     3361 cggtgagtcg tccctttcgt caaccttgcg gtcgtctttg agaagggcct cgcggcgaag
     3421 ctgttcggcg ttctgttcgg gcgtcggctg ggggtttggg ctctgttcgg gcatcgcagc
     3481 cgcagggccg ggcttgccgc cactcggcca aaagcgcagt ttttgcaggt atttcttcat
     3541 agatcggggc gccgagattt gaactcggga cctctagtcc cccagactag cgcgctaacc
     3601 aggctgcgcc acgccccgtg gcggcgtcat cgtatccgct acgcgccgta acgcttgtac
     3661 agcctgtcga tcagatccgg catgcgctcg agccggtcgg tgatcacacg caaccggtat
     3721 tcccggaagt gctcggcttc gacgcccaag gctcgcgcga gtgtcttgat cacatcgtcc
     3781 gacggaacgg ggcggttgcc gtggaccaga tgattgagat agcccgccga aagcctcgtc
     3841 ttctcggcca gtgacctgta cgtcagtccc gcctccgcca tcagccgctc gatggtcggg
     3901 ccgaacgagt cctcactgaa acgccgcttt cgtgccatca gccgagtttc tcgcgcaccg
     3961 tagagacgag ctcacccacg ctcaccgcct tgtcttcccc ctttgcgcgc tcgcgaatgt
     4021 ccaccgtacc gtcttccgcg gcctttcgcc cgaccgtcac gcgcaccggg cagccgatca
     4081 gatcggcgtc cgcgaacttc tcccccgctc gcgcatcccg gtcgtcgacg agaacgtcga
     4141 agcccgccgt agagagcgca tccgccaccg gctgcgcatg ctcctccatc cccgggatga
     4201 cgacaacatg gacgtcgtat ggagcgacgg aggcgggcca ctgaatgcct ctctcgtcgt
     4261 gatgctgctc gacgatcgcc gccattgtgc ggccaggccc gattccgtaa ctgcccatca
     4321 cgagcggctt ctcctctccg tcctcgtcaa ggaacgtcgc cccgaagcgg acggagtaga
     4381 aggtcccgag cttgaagatg tgcccgagct cgatggccgg ctgctcgatg agccttccgc
     4441 cgcagacgac gcacgcgtcg ccttggttcg cctgccggat gtcggcgaag ctcggggtgt
     4501 agtcgcgccc ggcctgcacg ccgcgcaggt gccatccgtc gcgattcgcg ccgccgacga
     4561 actgcccctc gcgcagcgcc tcgtcggcga cgatgtcgat cgtcaccccg accggcccaa
     4621 gcgagccgcc gccggcgccg aagaccgccc tgatctcttc gccggtcatc ggccgatagg
     4681 cctcgccgag cgtcgtgacg agcttctcct cgctcagccg gtcgtcaccg cggatgagcg
     4741 cgagcaccgg gcgctcgccg atcatcacgg gcatcgcctt cgacgtcgcc gccgggtcga
     4801 tgccgaggaa ctcggccaac gcctcgatgg tcgtaacgcc cggcgtctcg acctcctcgg
     4861 gctgctcgag cggctcgggg aactccggcg cacgcgggat tccacgcgcg gcgtcgtagt
     4921 cggcgtagta gtcgccgttc tcgcagcgga cgaggacgtt ctcgccggac gcggcgggcg
     4981 ccatgaagcc cgacgactcg cggccaccca tgattccgga ctcggcttcg acgtagtacg
     5041 cctcgagccc gcagcgctgc cagatgcgct cgtacgcagc cttgtgcgcg tcgaagctct
     5101 tgtccagccc ggcttcgtcg cggtcgaacg agtaagagtc cttcatgatg aactcgcgca
     5161 cgcgcaagag cccgccgcgc ggccgcggct cgtcacgctc cttcgtcgag aagtggtacc
     5221 agagttgtgg gagctgcttg tacgactgca gctcgcgcgc gtggaaggtc atggtctcct
     5281 cgtgcgaaaa cgggaggacg aagtctcgcc ccgcgcggtc cttcagcttg aagacgatgg
     5341 ggattcgaat ccggccgctc gtctcccaga gttcggccgg cgtcagcacc ggctcgagca
     5401 tctcctgccc gccgattgcg ttgatctcct cgcggatgat ctggacgacc ttctgatgga
     5461 cgcgccagcc cagcggtaga aacgtccaca atccagcacc aacctggcgg atgaacccgc
     5521 cgcgcaccag cagcctgtgg ctcgcagcct ctgcgtccgc cggcggctcg cgcagggtcg
     5581 gaagatacag ctgtgaggcc cgggcaatca tgttgtccgg gcagactaat cggagagcgg
     5641 aatcagtgac gcttccgcca tcgcgagcgc agcgggcttc gccatcttca gacgcttggg
     5701 acgttccatc ccgtcggcga tccacttgtc gatctcgtgg aacagctcgt cgatgaggat
     5761 gtcggacgag accttcttca gcacgcgtcc gtgcgcgtaa acgaaacctg tgtcacgccc
     5821 gccggcgatg ccgaagtccg cgtcgccggc ttcgcccgga ccgtttacgg cgcaaccgag
     5881 caccgcgacc tcgaagtgct gcgggtatgc gtgcagccgc tcctccactt tctccgcgag
     5941 ggtttgcaca ccgacgttgt cgcggccgca cgatggacaa gcgatcatga tcgggccacg
     6001 ctcacgcagg cccagtgact tgagaatctc gaacgcgacg cgcacctctt ccacgggatc
     6061 cgccgtcagc gagacacgca tcgtgtcgcc gacgccgtcg acgagcagtg cgcccatgcc
     6121 gactgcgctt ttgatcgagc ccgagaaagg cgtgcccgcc tcggtgacgc cgagatgcaa
     6181 cgggtagggc accttcgagg cgagctggcg atacgcgcgg atcatcgtcg gcacatggct
     6241 cgacttcacc gagatcttga actctctgaa gtcgaggctc tcgagcaagc gcacttcctc
     6301 gagcgccgcc tcgacgagcg cctcggcctg gtccttctgc gcgagctcgt tgagatgctt
     6361 cggcagcgac cccgagttcg cgccgatgcg catcgggatc ttcgcggcct gcgcggcgcg
     6421 cacgacctgc gcgactttgt cggagccgcc gatgttgccg ggattgatac ggaccgccgc
     6481 gacgccggcg tcgatcgcct tcagcgcgag gctcgcgttg aaatggatgt cagcgatgac
     6541 cgggagcggc gagagccgga tgatccggcc cagtgcctct gcgtcctccg tcttggggac
     6601 ggccacgcga acgatctcgc agcctgccga tgccagggca gcgatctgcg cggtcgtggc
     6661 ctcgacgtcg tgcgtcttgg tcagcgtcat cgactggact acgacggggg cgccgccgcc
     6721 gatcttcacg ccgccaaccg agatctggcg ctcgctggcc atcgattcca gtgtatagcg
     6781 gccctcaggg ggtcagacac ccatgcctaa gtgtcaccaa gtcgtaacga aactcaccga
     6841 ccccgcccat ggagccgagt cctgggtgcc gcgacttcgc aggctggtgt cagacaccgt
     6901 gccgggtgtg tgacgagagg gctagccgcc gccgaggtgg ttgatgtcgt tcgacaggcc
     6961 gatgaacgtg agcacaagga agagggcgat cccgatcgcg gacacgcgct cgtagacctc
     7021 gcgcccgact gctcgaccgc ggaggccctc gatgatcgaa aacaggatgt gcccgccgtc
     7081 gagtggcagc agcggcaaga ggttcaggat cgcgagtgag atgctgatca gcccgaggat
     7141 ctgtaggtac agcgtgaacg tgagcgagac cgcctggctc gagacgtcca cgatcccgac
     7201 cgggctcgag acctgctttc gtcccgcccc cgttgcaagt ctgggtaaga acgtgatcgt
     7261 cgccttcgtc gtctcccagg tgatgctgag actctttgtc aacgccttga cggggccgta
     7321 gctcacttcc ttccagcccg gctggaatcc gagtgcccag cgactgccga tctgtttcgt
     7381 ccggaccggg ccgagcatga ccgctttacc gttgcgcaaa acggtgactg tgatcggctt
     7441 gcccttcgac gctcggatca gccgagagac agtgtcgaag ttcggcgtct cgcggccgtt
     7501 caccgcgatg atcgtgtcgc ccgcgtggag cccaacggac gcggcgggcg tgttggcctt
     7561 gacgttcttc accgtccgat cgggatctcc cgggacgccc aggaggtacc cgagcgcgac
     7621 gagcacgatt gcgacgacga tgttgacgcc ggggccggcg aagatgacgg cgacgcgctt
     7681 ccacgtccgt tgccgccagt aggcgtccga gctcagcccg tcttgcacct cctcgacgga
     7741 acgtcgtgcg gcctttcccg ccgagggact gagggtcgcc tcttcgaccg cgcgctcgag
     7801 ctcgggaagc gcgtctcggg cgctgtcgag atcgttcgcc tcgagcccgc gcttgacgcg
     7861 cgcgaccttc gggaagaggc tcgggtcttc ggcgagcgcc ggcgcgagct gcacgtccag
     7921 gtcggacgca gccggacggt gcatgcccgg gatcttcacg tagccgccga gcgggatcgc
     7981 tccgattccg tattcgatgc cgttgcgcac gaccttcacg agcgccggcg ggaaaccgag
     8041 gtagaaccgg cggggcctca taccaacggc gcgcgcaacg aagaagtggc cggcctcatg
     8101 cacgaggatc agaagcgcga ggcccaggat cgcgacgaag atgctcatcg gccggtcccc
     8161 ggtcgatgag cggtggaagg ggcgtaaggg gaaaccggga ggtttccccc ctctttctcc
     8221 tcggcgttcg cggcggcggt cgccatgccg tgggcgctga ggcgatccct aaagaaggga
     8281 g
//
LOCUS       DASVEU010000176        16552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_120695,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000176 DASVEU010000000
VERSION     DASVEU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16552
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120695"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VF225_07185"
     CDS             <1..861
                     /locus_tag="VF225_07185"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX7145078.1"
                     /translation="IAAIQENWQQRRETLLSVDEAIASIVETLRQSGELDNTLILFTS
                     DNGFFHGEHRVANGKVLWYEPSIHLPLLLRWTGNTALPRGVHRSQLAMNVDDAETILD
                     AAGVRAGRTEDGVSLLPFWRDRGKEIGRDLLLDNSPGAGHFDGIRTLHYKYAEYANGD
                     RELYDLRKDPYELQSQHANPAYDALKASLAARLHNLVACAGPACRARPAVRFTAGRKG
                     GCGVVTATVAGQGVQNASFFVDGRRVLTDYRSPFRAKVHFKKRTVVRARVALAFDRLV
                     TLDQVVRACR"
     gene            complement(996..1736)
                     /locus_tag="VF225_07190"
     CDS             complement(996..1736)
                     /locus_tag="VF225_07190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7145079.1"
                     /translation="MTRLYEDDAELYDIAFDWDISDEADWLVARLEATTVLEPGCGSG
                     RMLEALADRGCQVVGIDRSPRMVELAGRRLGTRGEVFEADMTHFDLGRAFDGAVCPIN
                     TLLHLTPAELGRHLHRMSLHLERRSRYLVQVGLLEVDHEPPAGSHWEASRGEISLRVE
                     WTDDELDLVSGTSRQRSRIEVLSGPRAGEVVEEVHEMTAWTPATWAEAIAASPFEQVA
                     TYDGGAKGKWPRVDATATGGLLWHELTL"
     gene            1777..3228
                     /locus_tag="VF225_07195"
     CDS             1777..3228
                     /locus_tag="VF225_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145080.1"
                     /translation="MEVERQHHNVTLAILTLAGIAFALQQTMVIPALPTLQRDLHTTT
                     TWVTWVLTVFLLVASVATPILGKLGDQYGKERLLVISLALFLIGSIGAATAWNIWSLI
                     AWRALQGAGSAVFPLSYGIIRDEFPREKVGVAIGLVSAVFGIGGGFGIVLSGVIVDNV
                     SWRWLFIVGAVGIAAALVLVHRFIPESPIKTPSRVDYLGATLMSGGLIAMLVALTEGE
                     SWGWTSGRTLGLAAAAIVLLVAWGSAELRVDEPMVDMRMLARREILFTNITALISGFA
                     MFGTFVLVPNFVEMPHGLSAAVQHAVDYGFDASATKAGLYLLPSSFALLFAGPLAGLI
                     GRRVGFKWPLASGMLLVSISAGSLTEWHDRPWHILAAMPVLGVGVGFAFAAMATLITE
                     AVRPTETGVATGMNTVMRTVGGVVGGQVGAALLAAHTIPGTNNVPSVDGFKIAFGIAA
                     VVALIGAAVAVFITPPRLRRREQLVVAATEATN"
     gene            3237..4010
                     /locus_tag="VF225_07200"
     CDS             3237..4010
                     /locus_tag="VF225_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145081.1"
                     /translation="MDEGLRLHLALCTVAERLAGVMERARFERRDGYVLMTFPTLPIP
                     SFNGVWADEDSAAVALGDALAEVEAEGIAAGVLTRSGDTPAVEEAARALGLTAAEPIP
                     GMVANAGDLDDAASELEIVRVDTADGFAQALALATEGFGLPADFLAPLYMLEVTGLAG
                     FEVYLGRVEGRDVTTAASYVVDGNVGIFNVATPSMHRGRGYGAAITWHAVREGLAAGA
                     DLAYLQASAIGESVYRRLGFREVVTYLLLTRPAEVTAIS"
     gene            4083..4274
                     /locus_tag="VF225_07205"
     CDS             4083..4274
                     /locus_tag="VF225_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145082.1"
                     /translation="MFRPKHTALLAGVVTTMALGGAGVAIAASGGNSTTKSSGATTTA
                     TTTTSQSNSSHCDHDNSGT"
     gene            complement(4340..4783)
                     /locus_tag="VF225_07210"
     CDS             complement(4340..4783)
                     /locus_tag="VF225_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145083.1"
                     /translation="MPQNPPEGYPSVMPYLLYEDSDAAVEFLVMAFGFTEKLRMTDDD
                     GRVNHAEVQMNDGVVMLGTPPGEYKNPAKLGGKTQSVYVYVDDVDTHYERAKQAGAKI
                     VREREDQFYGDRNYGVQDPEGHEWYFGTHVRDVSAEEMQTAGAAN"
     gene            complement(4848..5375)
                     /locus_tag="VF225_07215"
     CDS             complement(4848..5375)
                     /locus_tag="VF225_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7145084.1"
                     /translation="MAPPRLTVPVSERDHVVGPQTAPVTIVEYGDYECPFCGAAHPVV
                     KALQKALGDNLRFAYRHFPLSQIHPHSFQAAEAAEAAGAQDRFWQMHDMIFEHQNRLG
                     TQDLLAYASAVGVELEPFAEDLAEHRHAPRVREDFLSGVRSGVNGTPTFFVNGVRHDG
                     GYDLESLLGAVRQHM"
     gene            complement(5384..5884)
                     /locus_tag="VF225_07220"
     CDS             complement(5384..5884)
                     /locus_tag="VF225_07220"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7145085.1"
                     /translation="MADYPDRALEAGTPAPDFQLKATPDQWVGLEDFRRKALIMAFYP
                     ADWSPVCTDQMSLYNEVLPMFHEYGAEIVGISVDSAWCHAAFAQHRKLHFPLLADFEP
                     KGEVAKRYGAYEYEEGVARRALFVLDAEGVIRWSYVSPMGVNPGAEGILAALEQMQEQ
                     PVGASR"
     gene            complement(5993..7969)
                     /locus_tag="VF225_07225"
     CDS             complement(5993..7969)
                     /locus_tag="VF225_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145086.1"
                     /translation="MCAGAVAVGIPTKDAAADAQVCGPSGYKLSLQSLTGPPRADLII
                     RISAKTGGCELPATLNEVQVAVLRFKRLPGRKRMLRNVPAPGGIATVDIGRVQRLRLV
                     RATVSFGPQVVLAAKAKTLLKPDLSLTRAYAGRSAVVGRPFFVVAIVHNRTKDVGLGA
                     VVTVSAVGAPLATKTVKVGPRRRVVLQLPVTLAQLGTTALTVTVTPSSPIETTVKNNT
                     RGLAVETAEFRVQESATLAQSFAGYGGQFNHHVYAAISRAAGVTDENAKDMEQKMRDL
                     HPQFSRIFFNNTAFTDPDRMQSFVRTAQLAQSTGTTINVTWQGGTLSVDNGTIPKFAA
                     VLIDLVHNRGITNLRWLTLQNEPNRTRITMAQYEAQYRALDPYIQSIRGQVKYMGGDL
                     VRAPDVGPPNQQAWFQYMATKMSDLLDAYSIHVFWDYFDTQKLVDRLTEVRAIVNALP
                     QAGRKPLYVSEYGIRGLRTFNGVPAGDPGVWEDGTPMGQTNINAFQHAWFDVLAAELG
                     YVGTSKWDSYFGKYDNGTQAYYMIGSPAEGWPLYPIYNSVRLLTSSVGAGWRTVNVAS
                     VPNTSRLLAGYVGKGGEHTVVGLDTAGAQLNTVSPTAISYTIGGLPPSKSFQLTIWNA
                     IGDGLVGQPTPAATDAAGVVTISVPQQAVFALRS"
     gene            8226..9143
                     /locus_tag="VF225_07230"
     CDS             8226..9143
                     /locus_tag="VF225_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145087.1"
                     /translation="MAADPNRFLNRAAEAKRSSKRVELVERFDSQAPGVWVELVRDVA
                     ALANSGGGVVVLSGDVEEERLHEELARYAEPEFEAFELHDITRARRPATAVVVEGVAN
                     TPLVFTRTGRYGTANGDEHVAFVRGGLYFRHGAKSEPATGADVRDFIKRQLDATRSQW
                     LANIRQVMHAPDGAEVAVIETAERDDEGRPTLIRLTTDPHAPLYGQVDPDRSHPYRQK
                     EVIREANARLDGAVVNAFDVLSVRRVHAISEETRPEFVHVPKFGSPQYSDAFVDWLIG
                     EHERDSEFFTRAKAKYLATRPRRRASTTS"
     gene            9205..9555
                     /locus_tag="VF225_07235"
     CDS             9205..9555
                     /locus_tag="VF225_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145088.1"
                     /translation="MAGKPVHFEVPAKDTTRAREFYSNLFGWQFESMEGPVEYHMTRL
                     SEDTGGAVFPGDPGAIRVYFDVDDVNAGVVRVKELGGKADDPQPVPGMGWFATGTDTE
                     GNPIGLWQTDSAAA"
     gene            complement(9566..10561)
                     /locus_tag="VF225_07240"
     CDS             complement(9566..10561)
                     /locus_tag="VF225_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX7145089.1"
                     /translation="MATNGKLTAKDYKTDQEVRWCPGCGDYAILAALQSFMPELEVPK
                     ENIVFVSGIGCAARFPYYMETYGMHSIHGRAPAIATGLATSRPDLSVWVVTGDGDSLS
                     IGGNHLIHALRRNVNVKILMFNNEIYGLTKGQYSPTSPLGTRTKSTPMGSLDWPFNPL
                     SLAIGAEASFVARAIDTDRAHLTETLRAAAAHRGSAFVEIFQNCNIYNDGAFDFVRED
                     KTNRIYLEHGKPIRFGEENERGVRQRADGSVELVDVTDESELLVHNAHHVEPSAAFAL
                     SRLTQSTANATPIGIFRDVERPVYDDLMAEQLATANAAQEGDLAALLQAGDTWTI"
     gene            10776..11183
                     /locus_tag="VF225_07245"
     CDS             10776..11183
                     /locus_tag="VF225_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7145090.1"
                     /translation="MARLRGSCLCGGVKFEVTEAPVTLRLCHCESCKKLSGGAATVNG
                     RVPSTAIRVLEGQELLRTFQPAEGSAKTFCVACGSNLFGGGWPDSEIASVRATALDDG
                     FAARPSAHIYVRSVAAWETLPADGLDRFETTPG"
     gene            complement(11180..11809)
                     /locus_tag="VF225_07250"
     CDS             complement(11180..11809)
                     /locus_tag="VF225_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7145091.1"
                     /translation="MSAADRLRHLWDFYDLDATEQRLRAQLEHESEAAGRAEVLTQLA
                     RVEGLRNDFDACDRLLEQAEAQAGSSAVAKVRIDLERGRKLRSSGDPEGALPLFEAAF
                     ARAWDSGEHYLAGDAAHMCALAVSDDRAGMEEWTQRGLELAEREPDAAYWAGPLLNNL
                     GWAYFDAGEHELALELFERALEARKRDPDNQDAIAFAEDAVETARAALG"
     gene            complement(12257..14098)
                     /locus_tag="VF225_07255"
     CDS             complement(12257..14098)
                     /locus_tag="VF225_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX7145092.1"
                     /translation="MASKPVEQLDSVVIRFAGDSGDGMQLTGGRFTSETALIGNDLAT
                     YPDFPAEIRAPAGSLPGVSGFQLHFADHDILTPGDQPNVLVAMNPAALKTNLNDLPAS
                     GILVVDKDAFTDRNLTKAGYAGNPIEDGSLDSYEVHAVPLTSMTIEALKDIEGITKRE
                     AERAKNMFALGLMSWLYGRPTEGTIAFLESKFAKRPEIAAANVKAFQTGYAFGETTEA
                     FAVQYEVKPASMKPGLYRNITGNQALAYGLIAASVKSGLPLFLGAYPITPASSVLEEL
                     ARHKNFGVRTFQAEDEIAAVGAALGASFGGSLGVSTSSGPGIVLKQETIGLGITLELP
                     LVIVDVQRAGPSTGMPTKPEQADLLGVMFGRNSESPVPVIAAATPSDCFDAALEAARI
                     AVKYRTPVFLLSDAYLANGSEPWLLPDVDAIALIEPNFAEANGEDFQPYRRDEQTLAR
                     PWAIPGTPGLEHRIGGLEKQDVTGNVSYDPDNHDLMVRLRAQKVAGIAQDIPELEVDD
                     QEGARTLVLSWGGTYGSVAAAVRRVRATGKKVAHAHLRYLNPFPRNTGEILRAYDKVL
                     VPEINLGQLQKLVRAEFLVDATGFNLVRGIPFRAGEVADAIDAMVDS"
     gene            complement(14167..14841)
                     /locus_tag="VF225_07260"
     CDS             complement(14167..14841)
                     /locus_tag="VF225_07260"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEX7145093.1"
                     /translation="MRSSRRRLATVLGLFVASLFCVALVLVRNLHTGNVNFRYLIWNL
                     FLAWIPFALAVFVYDRWRRRGGGLLLLFLGGLWLLFFPNAPYIATDFVHLQHDPLSPY
                     WYDAVTIAAFAWMGVLLGFASLYLMQTVVRQWRGAIAGWIFAFVAIALGSLGIYLGRF
                     LRLNSWDALEHPSVLPRILHTVARDPFAYQEAIAVTILFTVALSFAYFLLYNFAAVGL
                     ELDTER"
     gene            complement(14845..15324)
                     /locus_tag="VF225_07265"
     CDS             complement(14845..15324)
                     /locus_tag="VF225_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145094.1"
                     /translation="MAIDPRVDIGHVHLKVSDIDRALAFYRDVLGFEEMQRYGDQAAF
                     ISAGGYHHHIGLNTWESKGGSPPPTGTTGLYHVAIRYPDRKALAQALKQLVEAEIPLS
                     GATDHGVSEAIYLRDPDDNGIELYRDRPPDEWPRAPDGSMAMISEPLDLQKLLVEAG"
     gene            complement(15349..>16552)
                     /locus_tag="VF225_07270"
     CDS             complement(15349..>16552)
                     /locus_tag="VF225_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145095.1"
                     /translation="AREPVLPLGLFRNKAFATSSVVGFVVGFGMFGALTYLPLYLQIV
                     KGVSPTRSGLQLTPLMLGLLVTSVLSGQLISRWGKYRIFPIIGTAVITVGMLLLSRLG
                     VGTSLWMAALDMVILGLGLGMVMQVLVLAVQNAVDYAYLGVATSGSTMFRSIGGSIGV
                     SLFGAIFSNRLHTELAQRLAPSVHLPSTTSPAAIRALPPPVHDSYVHAVTAALSPIFF
                     VAAVICLVAFVLALLLPDVPLRQTSAAEGIGETFATPSHAGSERELERVLSIIARRDE
                     RWRAYAEWVNRAGVDVDPREAWLLARTRERAPASVPQIAEATGVDEEAVRRTMERLEQ
                     RGLVVERQGDYELTPEGQRVCEALVAVRRATLEDLVRDWSPADHAQLSPVLDRLARSL
                     ASEMPTPA"
BASE COUNT         2745 a         5455 c         5579 g         2773 t
ORIGIN      
        1 atcgcagcga tccaggagaa ctggcagcag cggcgcgaga ccttgctgtc ggtcgatgag
       61 gcgatcgcgt cgatcgtcga gacgctgcgc cagagcggcg agctcgacaa cacgctcatc
      121 ctcttcactt ccgacaacgg gttcttccac ggcgagcacc gcgtcgcgaa cggcaaggtg
      181 ctgtggtacg agccgtcgat ccatctgcct ctgttgctgc gctggacggg aaatacggcg
      241 ctcccgcgcg gcgtccaccg cagccagctg gccatgaacg tcgacgacgc cgagacgatc
      301 ctcgacgcgg cgggcgtgag ggccggacgg accgaggacg gagtgtctct gctcccgttc
      361 tggcgcgacc gcggcaaaga gatcggccgc gacctcttgc tcgacaactc ccccggagcg
      421 ggtcacttcg acggaatccg cacgctgcat tacaagtacg cggagtacgc gaacggcgac
      481 cgcgagctct atgacctgcg caaggacccg tacgagctgc agagtcagca cgcgaatccg
      541 gcctacgacg ccctgaaggc gtcgttggcg gcgcggctgc acaacctcgt cgcctgcgcg
      601 ggcccagctt gtcgtgcacg tccggcggtg cgcttcacgg cgggaaggaa gggaggctgc
      661 ggtgtcgtca cggccaccgt ggccggccag ggtgttcaga acgcgtcgtt cttcgtcgac
      721 gggcggcgcg tcctgacgga ctaccgctcc ccgttccgcg cgaaggtgca cttcaagaag
      781 cggacggtag tgcgtgcccg cgtcgccctc gccttcgacc ggctcgtgac gttggatcag
      841 gttgtgcgcg cctgccgcta gcaggctcgg tcgatagagg cacgtcccga acagcaaggg
      901 tctgaccccc agcctttgct ggcgtggctc gtggcggcac gtcccgaacg gcaagggtct
      961 gaccccagcc tttcgtggca tgccacgaag agcgactaga gcgtgagctc gtgccagagg
     1021 aggccgccgg tcgcagtcgc gtctactcga ggccacttcc cctttgcgcc accgtcgtag
     1081 gtggcgacct gctcgaacgg ggacgccgcg atcgcctcgg cccacgtagc cggcgtccaa
     1141 gcagtcatct cgtgcacctc ctcgacgacc tcgccggcgc gaggccctga caggacctcg
     1201 atgcgggatc gctggcggga ggtgccactg acgaggtcga gctcgtcgtc ggtccactcg
     1261 acccgcaggc tgatctcccc gcggcttgct tcccagtgag aaccggcagg cggctcatgg
     1321 tcgacttcga gaagaccgac ctggacgaga tagcgcgagc ggcgctcgag atgcagcgac
     1381 atccggtgga gatgccgccc gagctcggcg ggggtgagat ggaggagcgt gttgatcgga
     1441 cacaccgcgc cgtcgaaggc gcgtcccaga tcaaaatgag tcatgtccgc ttcgaacact
     1501 tcgcctctcg ttcccagccg ccgaccggcg agctcgacca tccgcggcga gcggtcgatg
     1561 ccgacgacct gacaacctcg atcggcaagc gcttcgagca tgcgtccgct gccgcagccg
     1621 ggctcgagaa cggtggtggc ctccaatctc gccacgagcc aatccgcctc atccgagatg
     1681 tcccagtcga aggcgatgtc gtaaagctct gcgtcgtctt cgtagagcct ggtcaccgga
     1741 cgatcctaag gcgcgtcggt aggctgaagc ggcccagtgg aagtcgagcg ccagcaccac
     1801 aacgtgacgc tcgcgatcct cacgctggcc gggatcgcgt tcgcactcca gcagacgatg
     1861 gtgatccctg ctctgccgac cctgcagcgg gacctgcaca cgaccacgac ctgggtgacc
     1921 tgggtgctga ccgtgttcct gctcgtggct tccgtcgcga ccccgatcct cggcaagctc
     1981 ggcgaccaat acggcaagga gcggctgctc gtgatctcgc tggcgctctt cctcattggc
     2041 tcgatcggcg cggccacggc ctggaacatc tggtcactga ttgcctggcg cgccttgcag
     2101 ggcgccggct ctgccgtctt tcctctcagc tacgggatca ttcgcgacga gttccctcgc
     2161 gaaaaggtcg gcgtggcgat cgggctcgtc tcagccgtgt tcgggatcgg aggcggcttc
     2221 gggatcgtgc tctccggcgt gatcgtggac aacgtgtcgt ggcgctggct cttcatcgtc
     2281 ggagcagtcg ggatcgccgc ggcgctcgtc ctcgtgcacc gtttcatccc tgagtcgccg
     2341 atcaagacgc cctcgcgcgt cgactatctg ggcgcgacgt tgatgtccgg aggtctgatc
     2401 gcgatgctcg tcgcgctgac ggagggcgag agctggggct ggacgtccgg gcgcaccctc
     2461 ggactcgccg ccgccgcgat cgtgctactg gtcgcctggg gcagcgccga gctacgtgtc
     2521 gacgagccga tggtcgacat gcgcatgctg gccaggcgcg agatcctctt cacgaacatc
     2581 accgcgctga tctccgggtt cgcgatgttc gggacgttcg tgctcgtgcc gaacttcgtc
     2641 gagatgccgc acggactgtc tgccgcggtg cagcacgccg tcgactacgg cttcgatgcg
     2701 agcgcgacga aggcagggct gtacctgctg ccgagctcgt tcgccctgct gttcgccggc
     2761 ccgctcgccg ggctcatcgg tcggcgcgtc ggtttcaagt ggccgctcgc ctccggaatg
     2821 ctgctcgtct ccatctctgc cggctcgctg acggaatggc acgacaggcc gtggcacatc
     2881 ctcgccgcga tgcccgtgct cggcgtgggt gtgggcttcg cgttcgccgc catggcgaca
     2941 ctgatcacgg aagcggtccg gcccactgag acaggcgtcg ccaccgggat gaacaccgtc
     3001 atgcgcaccg tcggaggcgt cgtcggcggt caggtcggcg cggccctact cgccgcgcac
     3061 acgattcccg gcacgaacaa cgtcccatcc gtggacggtt tcaagatcgc tttcgggatc
     3121 gctgccgtcg tcgcgctgat cggcgctgcc gttgcggtgt tcatcacgcc gccgcgcttg
     3181 cgccggcgcg agcagctcgt cgtcgcagcc acggaagcca cgaactaagt cgtcgcatgg
     3241 acgagggcct gcggctgcac ctcgcactct gcacggtggc cgagcgactg gccggggtga
     3301 tggagagggc tcgcttcgag cggcgagacg gttacgtgct catgacgttt ccgaccctgc
     3361 cgattccttc gttcaacggc gtctgggccg acgaggactc ggccgcagtc gcgctgggcg
     3421 acgcgcttgc agaggtcgag gcggagggaa tcgcggcggg ggtcctcact cggagcggag
     3481 atacacctgc cgtcgaggag gcggcgcgcg cactcgggct caccgcagca gagcccattc
     3541 ccggaatggt ggcgaacgcc ggcgacctcg acgacgccgc atcggagctc gaaatcgtcc
     3601 gcgtcgacac tgcggacggg ttcgctcagg cactcgccct cgccacagaa gggttcggcc
     3661 tgccggcaga ttttctcgcg cccttgtaca tgctcgaggt cacagggcta gccggattcg
     3721 aggtgtacct cggccgggtc gaggggcgcg acgtgaccac tgctgcgagt tacgtcgtcg
     3781 acggaaacgt cgggatcttc aacgtcgcga caccgtccat gcaccgtggt cgcggttacg
     3841 gcgcagccat cacctggcat gccgtccgcg agggcttagc ggccggagcc gatctggcat
     3901 acctgcaggc gagcgcgatc ggcgaatcgg tgtaccgccg cctcgggttc cgcgaggtcg
     3961 tgacgtacct gctcctgacg cgccccgcag aagtcacagc gatttcttaa tcgcgtcttg
     4021 gcctgttctt aggcggcgga ggttcgatgg ttggaacgca ccgcgaccga aaggaggttc
     4081 acgtgttccg acccaagcac actgccctgc tcgccggcgt cgtcacgaca atggcactcg
     4141 gcggagcggg agttgcgatc gccgccagcg gcggcaactc gaccacgaag tcgtcgggcg
     4201 cgaccacgac agccacgacg acgacctcac agtcgaactc atcgcactgc gatcacgaca
     4261 acagcggcac ctgacgagca aacggccgcc ggcgtccaac tcgggcgccg gcggccgtca
     4321 aacctcctgt actacgcagc tagttggctg ctcccgccgt ctgcatctcc tccgcggaga
     4381 cgtcacggac gtgggttccg aagtaccact cgtgcccctc cggatcctgg acgccgtagt
     4441 tacggtcgcc gtagaactga tcctcgcgct cgcgcacgat cttcgcaccg gcctgtttcg
     4501 ctcgctcgta gtgcgtgtcg acgtcgtcga cgtagacata gacgctctgg gtcttgccgc
     4561 cgagcttggc cggattcttg tactcgccgg gaggtgtgcc cagcatgacc acgccgtcgt
     4621 tcatctgcac ctcggcgtga ttgacacgac cgtcgtcgtc cgtcatgcgg agtttctctg
     4681 tgaagccgaa cgccatcacg aggaactcga cagcggcatc cgagtcttcg tagagcagat
     4741 acggcatgac gctcgggtac ccctcgggtg ggttctgcgg catggtgttg ccccctttcg
     4801 tctcgctccc gccaaactac cccaggagcg gatcgaaagg ggtcaattca catgtgttgc
     4861 cgaaccgctc ccagcaacga ctcgagatcg taaccgccgt catgacgaac gccgttgacg
     4921 aagaaggtag gcgttccgtt gacgccgctg cgaacgccgc tgaggaaatc ctcgcgcact
     4981 ctgggagcat gacggtgttc tgcgagatcc tccgcgaacg gctcgagctc taccccgacg
     5041 gcgctcgcgt aggccaacaa gtcctgcgtg ccgagccgat tctgatgctc gaagatcatg
     5101 tcgtgcatct gccagaagcg gtcctgggct cccgctgctt cggccgcctc ggcggcctgg
     5161 aacgaatgcg ggtggatctg gctcaagggg aagtggcgat aagcgaagcg aaggttgtcg
     5221 ccgagcgcct tctgcagcgc cttcacgacg ggatgcgccg cgccgcagaa cgggcactcg
     5281 tagtcgccgt actcgacgat ggtgacgggc gcggtctgag ggccaacgac atgatcccgt
     5341 tcgctgaccg gcaccgtgag gcgcggcggc gccatcagca cgctcatcgc gatgccccca
     5401 caggttgctc ctgcatctgc tcgagcgccg cgagaatgcc ctcggctccc ggattcacgc
     5461 ccatcggcga cacgtagctc caacggatga caccctcggc gtcgagcacg aagagcgcgc
     5521 gccgtgcaac gccttcctcg tactcgtagg cgccgtatcg cttggcaacc tcgcccttcg
     5581 gctcgaagtc ggccaggagc gggaagtgca gcttgcggtg ctgggcgaat gcggcatgac
     5641 accaggcgct gtcgaccgag atgccgacga tctccgcccc gtactcgtgg aacatcggga
     5701 ggacctcgtt gtacagcgac atctggtcgg tgcagacggg gctccagtcg gcgggataga
     5761 aggccatgat cagcgccttc ctgcgaaagt cttcgagtcc gacccactgg tcgggcgtcg
     5821 ccttgagctg gaagtccggc gccggggttc ctgcctcgag tgcacgatcc ggatagtcgg
     5881 ccatgccgcc gttatagccg cgggccgggg taagagagct ccccgcggat accgcccggt
     5941 aacctcgtcc ttcgcaggcc gttgccccgg cggatgtgac cttcggcgtc ggctaactcc
     6001 tcaacgcaaa aacagcttgc tgcgggactg agatcgtgac gacgcccgcc gcgtcggtcg
     6061 cggccggcgt cggctgtccc acgagcccgt cgccgatggc attccagatc gtgagctgaa
     6121 agctcttgga cggcggcagg cctccgatcg tgtacgagat cgcggtgggt gagacggtgt
     6181 tcagttgcgc tcccgctgtg tcgaggccga cgacagtgtg ctcgcccccc ttgccgacgt
     6241 agcctgcgag aagccgcgac gtgttcggca ccgatgcgac gttcaccgtc ctccagcctg
     6301 cccccactga ggacgtcagc agccgcacgg agttgtagat cgggtagagc ggccagccct
     6361 cggcagggct gccgatcatg taatacgcct gcgtgccgtt gtcgtacttg ccgaagtagg
     6421 agtcccattt gctcgtgccg acgtagccga gctcggcagc gagcacgtcg aaccaggcgt
     6481 gctggaaggc gttgatgttc gtctgcccca tcggtgtccc gtcctcccag acgcccgggt
     6541 cccccgcggg cacgccgttg aaggtccgca ggccgcggat gccgtactcg gagacgtaga
     6601 gcggcttgcg acccgcctgt ggcagcgcgt tgacgatcgc gcgcacctcg gtgagccggt
     6661 cgacgagctt ctgcgtgtcg aagtagtccc agaagacgtg gatcgagtag gcatcgagaa
     6721 ggtccgacat ttttgtggcc atgtactgga accacgcctg ctgattcgga ggtccgacat
     6781 cgggcgcacg gacgagatcg ccgcccatgt acttcacctg tccgcggatg ctctggatgt
     6841 aggggtcgag cgcgcgatac tgagcttcgt actgcgccat cgtgattcgc gtccggttcg
     6901 gttcgttctg gagcgtcaac cagcgcaggt tcgtgatccc gcggttgtgg acgaggtcga
     6961 tcaggacggc agcgaacttc gggatcgttc cgttgtccac gctcaacgtc ccgccttgcc
     7021 aggtgacgtt gatcgtggtg ccggtgctct gggcgagctg tgccgtgcgg acgaacgact
     7081 gcattcggtc cggatcggtg aacgccgtgt tgttgaagaa gatccgcgag aactggggat
     7141 gcaggtcgcg catcttctgc tccatgtcct tcgcgttttc gtcggtgacg ccggccgcgc
     7201 ggctgatcgc tgcgtacacg tgatggttga actgcccccc gtagccggcg aagctctgcg
     7261 ccagcgtcgc cgactcctgc accctgaact cggcggtttc gaccgcgagc ccgcgcgtgt
     7321 tgtttttgac cgtcgtctcg atcgggctcg agggagtgac ggttaccgtc aaggcggtcg
     7381 tgccgagctg cgccagtgtg accgggagct gcagcacgac gcggcggcgt ggaccaacct
     7441 ttaccgtctt cgtcgcgagc ggggcgccga cggcggagac ggtgacgacg gcgccgaggc
     7501 cgacgtcctt cgttcggtta tgcacgattg cgacgacgaa gaacggacga ccgaccacgg
     7561 ctgagcgtcc ggcgtaggcc cgcgtgagac tcaagtcggg cttgagcagc gtcttcgcct
     7621 tggcagcaag cacgacctgc ggaccgaatg agacggtggc gcgcacgagc cgcagccgct
     7681 ggacgcgacc gatgtcgacc gtggcgatcc cgccgggagc cggaacgttg cggagcatgc
     7741 gcttgcgccc cggcagcctc ttgaagcgaa gtaccgcgac ctggacttcg ttcagcgtcg
     7801 ctgggagctc acagcccccg gtcttcgcgc tgatgcggat gatgagatcc gcgcgtggcg
     7861 gtcccgtgag cgactggagg gagagcttgt agccggatgg gccacacacc tgcgcatctg
     7921 ccgcggcgtc tttcgtcggg atgccaaccg cgaccgcacc ggcgcacacg ccgaccaaga
     7981 ctctccgaaa ggaccccatg acccgggcgc agcgtcgccg tagccgccag atgtgtcaaa
     8041 tcggccgatc gagccgcgta acgaagcggc gttctgttac gtcgaagtaa agatgggagg
     8101 cgagtcgtcc gcggaccggc tgttacgccc ccgtcaccgc ggtctgacca agtgcgcacg
     8161 tggtcgggta tgaaccgcat gggaggtgct ggccccgtga ggcctgcact ctttagattt
     8221 gcagtgtggc ggcggatccg aacagattcc tgaaccgcgc cgcggaggcc aagcggagct
     8281 ccaagcgggt cgaattggtc gagcggttcg actcgcaggc gcctggagtc tgggtcgagc
     8341 tcgtccgaga cgtcgcggcg ctggcgaact ccggcggcgg ggtcgtggtc ttgagcggcg
     8401 acgtggaaga ggagcgcctc cacgaggagc tcgcgcgcta cgccgagcca gagttcgagg
     8461 ccttcgagct gcacgacatc acgcgagcga ggcgaccggc gacggcggtc gtcgtcgagg
     8521 gggttgcgaa cacgcccctc gtgttcacgc ggacaggccg atatggcacc gccaacggag
     8581 acgagcacgt cgccttcgtg cgcgggggcc tctacttccg gcacggggcg aagagcgagc
     8641 ccgcgacggg cgcggacgtg cgcgacttca tcaagcggca actcgacgcg acgcgatcac
     8701 agtggctcgc gaacatccgc caggtgatgc acgcgccaga cggcgctgag gtcgccgtga
     8761 tcgagacggc tgagcgcgac gacgaaggcc gcccgacact gatccggttg acgacggatc
     8821 cgcacgcgcc cctctacggc caggtcgatc cggaccgctc gcatccgtac cgccagaagg
     8881 aagtgatccg agaggcgaat gcccgcctgg acggcgcggt cgtcaatgcg ttcgacgtcc
     8941 tcagcgtccg gcgagtgcac gcgatcagcg aggaaacgag accggagttc gtgcacgtgc
     9001 cgaagttcgg ctcgccgcag tacagcgacg ccttcgtgga ctggctcatc ggcgagcacg
     9061 agcgggattc ggagttcttt acacgggcga aggcgaagta cctggcgacg cgcccgcggc
     9121 gccgggcgag caccacttcg taaccaagac gctcgagtgg gacggatatc gtgggtctcg
     9181 accctgcgtc cggaaggagc ccccatggct ggaaagcccg tccacttcga ggtgccggcg
     9241 aaggacacga cccgcgcgcg cgagttctac agcaacctgt tcggctggca gttcgagtcg
     9301 atggaaggcc cggtggagta ccacatgacc cggctcagcg aagacacggg cggagccgtg
     9361 tttccgggag accccggtgc gatccgcgtc tacttcgacg tcgacgacgt caacgccggt
     9421 gtcgtacgtg tgaaagagct gggcgggaag gcagatgacc cgcaaccggt gcccggaatg
     9481 ggttggttcg cgaccgggac ggacacagag gggaatccca tcggcctctg gcagaccgat
     9541 tcggcggcgg cgtgagcgtg agcccctaga tcgtccaggt gtcgccggcc tgaaggagcg
     9601 ccgccaggtc gccctcctgc gcggcgttcg ccgtcgcgag ctgctcggcc atcaggtcgt
     9661 cgtagacggg ccgctcgacg tcccggaaga tcccgatcgg agttgcattt gccgtcgact
     9721 gggtcaatcg actgagggca aacgcggcac tcggctcgac atggtgtgcg ttatggacga
     9781 gcagctcgga ctcgtccgtc acgtctacga gctcgacgct gccgtccgcc cgctgccgca
     9841 cgcccctctc attctcctca ccgaaacgaa tcggcttccc gtgctccagg tagatccggt
     9901 tcgtcttgtc ctcgcggacg aagtcgaagg cgccgtcgtt gtagatgttg cagttctgga
     9961 agatctcgac gaacgcgctc ccgcggtggg cggctgcggc ccgcagcgtc tccgtcagat
    10021 gcgcacgatc ggtgtcgatc gcgcgcgcca cgaacgacgc ctccgcgccg atggcgagcg
    10081 acagcggatt gaaaggccag tccagcgagc ccatcggcgt cgacttcgtc ctcgtcccca
    10141 gcgggctcgt cggcgagtac tggcccttcg tcagcccgta gatctcgttg ttgaacatca
    10201 ggatcttgac gttcacgttg cggcgcagcg cgtgaatcaa gtggttgccg ccgatcgaca
    10261 gcgagtcgcc gtcgccggtc acgacccaca cggacaagtc tggcctcgac gtcgccagcc
    10321 cggtggcgat cgccggcgcc ctgccgtgga tcgagtgcat cccgtacgtc tccatgtagt
    10381 acgggaaccg cgctgcacag ccgatgccgg aaacgaagac gatgttctcc ttcggcacct
    10441 ccagctcggg catgaacgac tggagtgccg ccaggatcgc gtagtccccg cagcccgggc
    10501 accagcggac ctcctggtcg gtcttgtagt ccttggcggt cagtttgccg ttcgtggcca
    10561 tggaccgatg atagtcccgg gcctcccatg agaagatggc caggagtcgt gccttaaagg
    10621 ggaggccctc gcggggaacc gcgcgagcac actgcggcga gtgcccgccg cgggacgagg
    10681 gcctgcccct cgggccaggg tacgacggca agtagaggcg acgtcgccgg gcccaacgga
    10741 atcagccccc acgggcccgg cgctaagctc gagccgtggc caggctgcgt ggaagctgtc
    10801 tctgcggggg cgtcaagttc gaggtgacgg aggccccggt cacgctcagg ctctgccact
    10861 gcgaaagctg caagaagctg tcgggcggcg ccgcgacggt aaacggccgg gtgccttcca
    10921 ctgcgatccg ggttctcgag ggccaggaac tgctgcggac gtttcagccg gcagagggaa
    10981 gcgcgaagac cttctgcgtc gcttgcggtt caaacctgtt cggcggcggc tggccggact
    11041 cggagatcgc aagcgtccgg gcgaccgcgc tggacgatgg gttcgctgct cgcccgagcg
    11101 cacacatcta tgtccgttca gtcgccgcgt gggagaccct ccccgccgac gggctcgacc
    11161 gcttcgagac gacccccggc tagcccagcg cggcgcgggc agtttccacc gcgtcttcgg
    11221 cgaacgcaat tgcgtcctgg ttgtcgggat cgcgcttgcg cgcctccaga gcacgctcga
    11281 agagctcgag cgccagctcg tgctcgccgg cgtcgaagta cgcccagccg agattgttga
    11341 gcagcgggcc tgcccagtag gcggcgtcgg gctctcgctc ggcgagctcg agcccgcgct
    11401 gcgtccactc ctccatcccc gcgcggtcgt ccgacacggc gagggcgcac atgtgagcag
    11461 cgtcgccggc gaggtagtgc tcgcctgaat cccacgcacg cgcgaaggct gcttcgaaca
    11521 gcggcaaagc gccttcggga tcaccgctgg agcggagctt ccggccgcgc tcgaggtcga
    11581 tgcgaacctt cgcgacggcg ctcgatccgg cctgcgcctc cgcctgctcg agcaggcgat
    11641 cgcaagcgtc gaagtcgttc cgcaaaccct cgactcgtgc gagttgtgtg agcacctcgg
    11701 cgcgccctgc agcttccgac tcgtgctcca actgcgcacg aagtctctgc tcggtggcgt
    11761 cgagatcgta gaaatcccac agatgcctaa gccgatcagc cgcactcacc gtggtctcat
    11821 tttgacctct tgggggcgtg ggtttaagcc tcgactggtc cgtcaatcaa ctatccgcag
    11881 gggggagcaa cggacacccg tctctgaccg ggcgttcggt tttggcgatg tcctgcgtct
    11941 ggtgggagcc gggagcggaa cctgcggcct ccgcatttac ctcgcgcccg gggacggcgg
    12001 cgaggtaggg cctggctccc accttcacgc cggtcgactt gtcaccagcg gcgcttgccg
    12061 cggccgtggc cgttgccgcc gctgctcacc tttttcctca gcaggatgcg aatcagctgg
    12121 gtgagagcca caaccgtagc cgccacgcac aggctgaaac ccgtccaatt cttgtcgagc
    12181 ccgaaggcga ccgcgccggc acccgcgccg acgacgatcg cggcccagag ccacctgccc
    12241 tcgacaggca gtgtctttat gagtccacca tggcgtcgat cgcatccgcc acttcgccgg
    12301 cgcggaacgg aatcccccgc accaggttga agccggttgc atcgacgaga aactctgcgc
    12361 ggaccagctt ctgcagctgg ccgaggttga tctccgggac gagcaccttg tcgtaggctc
    12421 gcagaatctc gcccgtgttg cgtgggaatg ggttgaggta gcgcagatga gcgtgggcga
    12481 ccttcttgcc ggttgcgcgg acacggcgga cggccgcggc gaccgagccg tacgtgccgc
    12541 cccaactgag gacgagcgtc cttgctcctt cctggtcgtc gacctcgagc tcggggatgt
    12601 cctgggcgat gccggcgacc ttctgcgcac gcagccgcac catcaggtcg tggttgtcgg
    12661 ggtcgtagct gacgtttccg gtgacgtcct gcttctcgag cccgccgatc cggtgctcga
    12721 ggcccggagt gccgggaatc gcccatggac gcgcaagcgt ctgctcgtcg cggcggtacg
    12781 gctggaagtc ctcgccgttc gcctcggcga aattcggctc gatcagggcg attgcgtcga
    12841 cgtccggaag cagccacggc tccgagccgt tcgcgaggta cgcgtccgag agcaggaaca
    12901 ccggcgtgcg gtacttgacg gcaattcgcg cagcctcgag tgccgcgtcg aagcaatccg
    12961 acggcgtcgc cgccgcgatc accggcacgg gcgactcgga gttgcggccg aacatgacgc
    13021 cgagcaggtc ggcctgctcc ggcttcgtgg gcatcccggt cgacggcccg gcgcgctgga
    13081 cgtcgacgat cacgagcggc aactcgagcg tgatcccgag gccgatcgtc tcctgcttca
    13141 aaacgatgcc ggggccgctc gaggtggaga cgccgagcga gccgccgaag ctcgcgccga
    13201 gcgccgcgcc gaccgcggcg atctcgtcct ccgcctgaaa cgtgcgcacg ccgaagttct
    13261 tgtggcgcgc gagctcctcg agcacggagg acgcgggcgt gatcgggtat gccccgagga
    13321 agaggggcaa gccggacttc accgacgcgg cgatgaggcc gtacgcgagt gcctggttgc
    13381 cggtgatgtt gcggtacagg cccggcttca tcgacgccgg cttgacctcg tattgcaccg
    13441 cgaacgcctc ggtggtctcg ccgaacgcgt agccggtctg gaaggccttg acgttcgcgg
    13501 ccgcgatctc gggccgcttc gcgaacttcg actcgaggaa ggcgatcgtc ccttcggtgg
    13561 gacgcccgta gagccacgac atcaggccga gcgcgaacat gttcttggcg cgctccgctt
    13621 cgcgcttcgt gatcccttcg atgtccttca gcgcctcgat cgtcatcgag gtgagcggga
    13681 ccgcgtggac ctcgtaggag tcgagcgagc cgtcctcgat cggattgccc gcgtaccccg
    13741 ccttcgtcag gttgcggtcg gtgaacgcgt ccttgtcgac gacgaggatc ccgctcgccg
    13801 gcaggtcgtt gaggttcgtc ttgagcgcgg cggggttcat cgcgacgagg acgttcggct
    13861 gatcgcccgg cgtgagaatg tcgtggtcgg cgaagtggag ctggaagccg gagacgccgg
    13921 gcagcgagcc cgccggcgcc cggatctcgg ccggaaagtc cggatacgtg gccaggtcgt
    13981 tcccgatcag cgcggtttcg gaggtgaaac ggccgcccgt caactgcatc ccgtccccgg
    14041 agtccccggc gaaccggatt acgacggaat caagctgctc gacgggcttc gaggccacct
    14101 gctagttgta gcgcaaccgt ccggcggaga caggtcggag ggactggtcc ctcggagctg
    14161 cccgaatcag cgttcggtgt cgagctccag cccgaccgcg gcgaagttgt agagcaagaa
    14221 gtacgcgaag ctcagcgcca ccgtgaacag gatcgtcacc gcgatcgcct cctggtaggc
    14281 gaacggatca cgggcgacgg tgtgcaggat ccgcggcagg acgctgggat gctcgagcgc
    14341 gtcccaactg ttcagccgca ggaagcggcc gaggtagatc ccgagcgagc cgagggcgat
    14401 cgcgacgaag gcgaagatcc agccggcaat cgcgcctcgc cactgacgga ccacggtctg
    14461 catgaggtac agcgacgcga agccgagcag cacccccatc cacgcgaacg cagcgatcgt
    14521 gaccgcgtca taccagtacg gggagagcgg gtcgtgctgc agatggacga agtcggttgc
    14581 gatgtacggc gcgttcggga agaagagcag ccagagcccg ccgaggaaga ggaggagcaa
    14641 gccgccgccc cgtcgccgcc agcggtcata gacgaaaacc gcgagcgcga acggaatcca
    14701 ggcgagaaag agattccaga tcagatagcg gaagttgacg ttcccggtat gcaggttgcg
    14761 cacgaggacg agcgcgacac agaagagcga cgcgacgaac agccccagga cggtcgcgag
    14821 tcgacggcga ctggagcgca tcgttcagcc ggcttcgacg agcagctttt ggagatcgag
    14881 cggttccgag atcatcgcca tgctcccatc cggagcgcgc ggccactcgt ccggtggacg
    14941 gtcgcggtag agctcgatgc cgttgtcgtc gggatcgcgg agatagatcg cctcgctcac
    15001 gccgtgatcg gtcgcgccgc tgagcgggat ctcggcctcg acgagctgct tcagcgcctg
    15061 cgcaagcgct ttccggtccg ggtaacggat cgcaacgtga tagagcccgg tcgtgcccgt
    15121 cggcggcggc gacccgccct tcgactccca cgtgttgagg ccgatgtggt ggtggtaccc
    15181 gccggccgag atgaacgctg cctggtcgcc gtagcgctgc atctcctcga agccgaggac
    15241 gtcacggtag aacgcgagtg cgcggtcgat atcgctcacc ttgaggtgga cgtggccgat
    15301 gtcgacgcgc ggatcaatcg ccatgcgctt gagggtacag cggccctgtc aggcgggagt
    15361 cggcatctcg ctggcaagcg agcgcgcaag ccggtccagg acgggcgaga gctgcgcgtg
    15421 gtctgcggga gaccagtcgc ggaccaggtc ctcgagggtt gcgcgccgca ccgcgacgag
    15481 cgcctcgcag acgcgttgcc cctcgggagt gagctcgtag tcgccttgcc tttccacgac
    15541 caggccccgc tgttccaggc gttccatcgt ccggcgcacg gcctcctcgt cgacacccgt
    15601 agcctccgcg atctgcggca cggaagcagg cgcccgctcg cgtgttcgcg cgagcagcca
    15661 cgcctccctc ggatcgacgt cgaccccggc gcggttcacc cactccgcgt atgcgcgcca
    15721 ccgctcgtcg cgacgcgcga tgatgctcag cactcgctcc agctcccgct ccgagccggc
    15781 gtgactcggc gtcgcgaagg tctctccgat cccttccgct gcactcgtct gtcgcaaggg
    15841 aacgtccggc aggagcagag cgaggacgaa tgcgaccagg cagatgacgg cagcgacgaa
    15901 gaagatgggt gagagcgcag ccgtgaccgc gtgcacgtac gaatcgtgga cgggcggagg
    15961 taacgcgcgg attgccgcag gtgacgtcgt cgacggaagg tgcacgctcg gcgcgagccg
    16021 ctgcgccaac tcggtgtgca ggcggttgga aaagatcgcg ccgaacagcg atacgccgat
    16081 cgagccgccg atcgaccgga acatcgtcga gccggaggtg gcgacgccga ggtatgcgta
    16141 atcgacggcg ttctgtaccg cgagcacgag gacttgcatc accattccga gaccgagccc
    16201 gaggatgacc atgtcgagcg cggccatcca cagactcgta ccgactccca ggcgcgagag
    16261 cagaagcatt ccgacagtta tgaccgccgt cccgatgatc ggaaagatcc ggtacttccc
    16321 ccagcggctg atcaactgcc cactgagcac cgaggtcacc agcaagccaa gcatcaaggg
    16381 cgtcaactgg agtcccgacc tggtcgggct cacgcctttg acgatctgca gatagagcgg
    16441 caggtacgtg agcgccccga acatcccgaa ccccaccacg aaccccacca ccgacgacgt
    16501 cgcgaacgcc ttgttgcgga acagcccgag cggcagcacc ggctccctgg ct
//
LOCUS       DASVEU010000177         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_898799,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000177 DASVEU010000000
VERSION     DASVEU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898799"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF225_07275"
     CDS             complement(<1..407)
                     /locus_tag="VF225_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145096.1"
                     /translation="MSAIAVEGPTLNPARAPERPQRFLPISGILFGILLALALWLTSK
                     EPSDTASAKQVFAYWSDHGGGKMWLSILGLELAAVLLLTFGAALRGAIRSGEGATATY
                     SSLVFGGAILAAMGFATTSILPAAAGRAAADKAA"
     gene            complement(522..1172)
                     /locus_tag="VF225_07280"
     CDS             complement(522..1172)
                     /locus_tag="VF225_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145097.1"
                     /translation="MRIVIAEDTVLLREGLAGLLEDAGHQVVARVGDAEALLAVVAEH
                     EPDLAVVDVRMPPDYEDEGMRAAAAIRQSHPGTAVLVLSQHIETRHAVELVAAGGGFG
                     YLLKDRVLDVDDFLDAARRVSEGGSAVDPEVVTTLLSSKRADDTLGELTPREREVLAL
                     MAEGRTNAAIAKRLWLTERTVETHVRSILGKLGLSISGDDHRRVLAVLTYLRAPTV"
     gene            complement(1160..3166)
                     /locus_tag="VF225_07285"
     CDS             complement(1160..3166)
                     /locus_tag="VF225_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7145098.1"
                     /translation="MRIAPRLFAWGFALVAVALITGGLVLDRIAAVEHVPEAGAMWLY
                     PFLFCAVSIPAAVGVLIATRRPRNPIGWTLVLGALSLAPVLAASPYAWVALRAHPGSL
                     PGGSWAALLSSLWPVFFAWPLAVAFVFPDGRLPSSRWRPYAVFAAGSFGLLLFVLVTA
                     KQLERPFSDVPNPFPVRLPDALGPLRSFAWLAMFASLFVGTAAVRSRYKRSAGIKRLQ
                     MLWLAYAALLIPLGVVCFLLWGLLFGEPGDAVVAFLLGMEAAVALAVGVAVRRYRLYE
                     IDRLINRTLVYALVTASLGLLYGVISVVAGVVLGRGSAWATALGAVFVALAFRPLRTR
                     AQAVVDWRFARPRYEGLRRVRSFVDDVRDGRREPEEIGTVLAAALRDPSARLLFRLPA
                     SDTYADSRGHVVAEPPEGDGALTPIEHRGQEIGLLRHRRDLLDRPDLLHSILEAASLA
                     IEIARLRVEVRLQLVEVDESRARIVQAGYEERRRLERDLHDGAQQRLVTLGIVLRRLQ
                     RSLPRGAKVLGPALDSAVDEIGHAIADLRTIASGVRPPRLDDGLAAAFADLARAAPIP
                     IDVEATGERLPPHVEAAAYFVVCEAVTNAVKHASPSRVRVEATSAGGALRLLVADDGV
                     GGANPRGTGLVGLTDRVTAHGGTLLIESPLGAGTRIEAEFPCGS"
     gene            complement(3250..>3744)
                     /locus_tag="VF225_07290"
     CDS             complement(3250..>3744)
                     /locus_tag="VF225_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7145099.1"
                     /translation="HTGWTVDIETDESMYGFKFQETLAADGLVLGRRTYEGFAAAWPG
                     REGDFADAFNTMPKYVVSSTLEDPEWNNTTVLKGDVGNEVEKLKQEQDGDIVVHGSAQ
                     LVQTLVENDLVDEFRLMVYPLVLGSGKRLFGETSDKKPLRLVESKVVGDGVAILIYQP
                     VGSD"
BASE COUNT          656 a         1294 c         1252 g          542 t
ORIGIN      
        1 gcggccttgt cggcggcggc tcggccggcg gccgccggca ggatcgacgt cgtcgcaaag
       61 cccattgcgg ccaggatggc gccgccgaac accagtgacg aataggtcgc ggtcgctccc
      121 tcgccggagc gaatcgcccc ccgcaatgcc gctccgaacg tcaggagcag gaccgcagca
      181 agctcgaggc cgaggatcga gagccacatc ttcccgccgc cgtggtcgct ccagtaggcg
      241 aacacttgct tagccgacgc cgtgtcactc ggctctttcg aggtgagcca gagcgcaagc
      301 gcaagcagga tgccgaagag gatgcccgag atcggcagaa aacgttgcgg tcgttctggc
      361 gctctggccg ggttgagggt gggaccctcg acagctatcg cggacacgtc acgacctcct
      421 tgtggatggc gatttcgccc ttgcctcagg acggtagggc acccccgccg gcacttcgtc
      481 gtcgcaggca ctgaacgtgc ttcagtgcga gcgcggagat gtcagacggt aggagcgcgc
      541 aggtaggtca ggactgcgag cacgcgtcgg tggtcgtcac cgctgatcga gagaccgagc
      601 ttgccgagga tcgaccggac gtgcgtctcg acagtccttt cagtcagcca cagccgtttc
      661 gcgatcgccg cattcgttcg tccctcggcc atcagcgcga gcacctcgcg ctcgcgtggc
      721 gttagctcgc cgagcgtgtc atccgcccgc ttcgacgaga gaagcgtggt gaccacctcc
      781 ggatcgacgg cggagcctcc ttcgctcacg cgccgtgctg cgtcgaggaa gtcgtcgacg
      841 tcgagcacac ggtccttgag cagatagccg aagccgcctc cggccgcgac gagttcgacg
      901 gcatggcgcg tttcgatgtg ttgtgagagg acgagcacgg cggtgcccgg gtgcgattgg
      961 cggatcgcgg ccgccgcacg catgccctcg tcttcgtagt cgggcggcat acgcacgtcg
     1021 acgacggcaa ggtcgggctc gtgctctgcc acgacggcga ggagcgcttc ggcatcgccc
     1081 acacgcgcga cgacctggtg ccctgcatcc tcgagcagtc ctgcgagccc ctcgcggagc
     1141 agaacggtgt cctcagcgat cacgatccgc acggaaactc cgcctcgatc cgagtgcccg
     1201 ccccgagtgg gctctcgatc agcagcgtcc cgccgtgggc cgtcacccgg tcggtcagcc
     1261 cgacgaggcc ggttcccctc gggttcgccc cgccgacgcc gtcatcggcg acgagcagcc
     1321 ggagtgctcc gcccgcagag gtggcctcca cgcggacgcg tgacggggat gcatgcttga
     1381 ccgcgttcgt gaccgcctcg cagacgacga agtaggctgc cgcctcgacg tggggaggaa
     1441 gtctctcccc ggtcgcctcg acgtcgatcg ggatgggcgc cgcgcgtgcg aggtcggcga
     1501 aggctgcggc aaggccgtcg tccagccgtg gcggccgcac gcctgaggca atcgtccgca
     1561 gatccgcgat cgcgtgcccg atctcgtcga cggccgagtc gagagccggc ccgaggacct
     1621 tcgcccctcg cggaagtgag cgctgcagcc gtcgcagcac gatcccgagg gtgacgagcc
     1681 gctgctgcgc tccgtcgtgc agatcgcgtt cgaggcgacg gcgttcctcg tagccggcct
     1741 gaacgatccg ggcccgcgac tcgtccacct cgacgagctg cagcctcacc tccactcgga
     1801 gtctcgcgat ctcgattgcc aggctcgctg cctcgagaat gctgtggagg agatcgggac
     1861 ggtcgagcag gtcgcgcctg tgccgaagca gcccgatctc ctgccctcgg tgctcgatcg
     1921 gtgtcagtgc accgtcgccc tcgggcggct ccgcgaccac gtggcccctg ctgtcggcgt
     1981 atgtgtcgct cgccgggagc cgaaagagaa gtctcgcact cgggtcgcgc agcgccgccg
     2041 cgaggacagt tccgatctcc tcaggttctc gccgtccgtc gcgcacgtcg tcgacgaatg
     2101 agcggacgcg tcgcagacct tcgtagcgcg ggcgggcgaa acgccagtcc acgacggcct
     2161 gcgcccgagt gcgcagcggc ctgaaggcga gcgcgacgaa gaccgcgccg agggccgtcg
     2221 cccacgccga gcctctgccc agaaccacgc ccgccactac cgagatgacg ccgtagagga
     2281 gaccgagtga cgcagtcacg agcgcgtaga cgagcgtccg gttgatcagc cgatcgatct
     2341 cgtacagccg gtagcgcctc acggcgacgc cgacggcgag cgcaacggcc gcctccattc
     2401 cgagcagaaa cgcaaccacc gcgtcgccgg gctcgccgaa caggagcccc cagagcagga
     2461 agcagacaac acccaacgga atcagcagcg cggcgtacgc cagccagagc atctgtaggc
     2521 gcttgattcc cgcggaacgc ttgtagcggc tgcgcacggc cgcggtgccc acaaacaggc
     2581 tcgcgaacat cgccaaccag gcgaatgagc gcagaggccc gagggcatcc ggaagccgaa
     2641 caggaaacgg attcggcacg tcggagaacg gacgctcgag ctgcttcgcc gtcaccagca
     2701 cgaacaagag gagcccgaac gaaccggccg cgaagaccgc gtacgggcgc cagcgcgaag
     2761 acggcagtcg tccgtcgggg aacacgaacg caaccgccag tggccaggcg aagaagaccg
     2821 gccacagcga cgagagcagc gcggcccacg aaccgccggg aagcgatcct ggatgcgccc
     2881 tcagcgccac ccaggcgtag ggcgacgcgg cgagcacagg cgcgagggac agcgcgccca
     2941 ggacgagagt ccatccgatc gggttgcgcg gccgccgcgt tgcaatcagg acgccgacag
     3001 cggccggtat cgagaccgcg caaaagagga aggggtagag ccacatcgcg ccggcttcgg
     3061 ggacgtgctc cacggcagcg atccggtcga gcaccagccc acctgtgatc aaggcgactg
     3121 cgacaagcgc aaacccccag gcaaatagac gcggagcgat ccgcacgcgc tcattctccc
     3181 ccagcggtca gcgtgtggcc agcgtacttg caccggaatg tctagtcgcg gcgcaggagc
     3241 tagtcggagc tagtcggagc ccaccggctg gtagatgaga atcgccacac cgtcgccgac
     3301 caccttcgac tcgaccagtc gcagaggctt cttgtcgctc gtctcgccga agaggcgctt
     3361 gccgcttccc agcacaaggg ggtacaccat cagccggaac tcatcgacga ggtcgttctc
     3421 gaccagcgtt tgcacgagct gggcgctgcc gtggacgacg atgtcgccgt cctgctcctg
     3481 cttgagcttc tcgacttcgt tcccgacgtc acccttcagt acggtcgtgt tgttccactc
     3541 tgggtcctcg agggtcgagg agacgacgta cttcggcatc gtgttgaagg cgtcggcgaa
     3601 gtcaccctct cgccccggcc aggcggccgc gaacccctca taggtacgcc gcccgaggac
     3661 cagcccgtcg gccgccaggg tctcctggaa cttgaagccg tacatcgact cgtcggtctc
     3721 gatgtcgacg gtccagccgg tgtg
//
LOCUS       DASVEU010000178         9431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1093439,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000178 DASVEU010000000
VERSION     DASVEU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093439"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF225_07295"
     CDS             complement(<1..414)
                     /locus_tag="VF225_07295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7145100.1"
                     /translation="MKRRSYGRDAGLTLRIFTTASLLGLLYVVFAVVLFNVLSVGLIP
                     MILIVVGIAFFQYFTSDKLALAASGAKVVERDQAPELHDMIERLCAMADLPKPRVAII
                     DTDVPNAFATGRSPKHAAVAVTRGLWQRLEPQEVEG"
     gene            463..2322
                     /locus_tag="VF225_07300"
     CDS             463..2322
                     /locus_tag="VF225_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7145101.1"
                     /translation="MGLATAGFLGVSGTAQARPKVYVIPSQGVLVTREFGAIGLYVPG
                     AGGTVSRAGAIAAIRRGKVESAALGGKPHGKILVDIVFGVPDLQVKNAVLVELPPPGG
                     HPNTKRYSVMIFGAGYHGILTSESTRIRGLVSIADLGPTAVALQEGRTPQIRAEPDRD
                     AREDLRALDTRLTRVHHDRGWVFVTVVLTILALTIFAPRAAGLGGAAAFGASLLLSWA
                     GATRLGLVIATIAALTIALAVAGSFRRSIVPPVVAGFLVALTVVLAVDPELNSLAVLG
                     ARPDGGGRFYGIGNQVETLLLAPVLMAAAIGGLRWLVPLGAVALVAIGWSKAGADGGG
                     VLVFAAALGVLALRLRGLAVTPRRLALVAVAVLVVAVAFVGLDAALGGSSHVTRALGS
                     GPDSLLGGLGHRLHLSWAAVTDRWFHGVIFLSCLAALVLMGTIRPRRATVDALLAGLA
                     VSLLVNDTPVDVIGLGALGCWALLRWESVDSRPMRRGVLIAAAATAAVLALAGCGSEG
                     VVRALPSTVVGTVKTEAPGKAIFISQGCGACHTYKPAGPGANGKIGPDLDKLPQYAKA
                     ANQPLATFVHDSIVDPNKYVEKGYPKGVMPTTYKSLSATDLKALVDFLTKPQG"
     gene            2358..3146
                     /locus_tag="VF225_07305"
     CDS             2358..3146
                     /locus_tag="VF225_07305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX7145102.1"
                     /translation="MACDLDRTLIGEDMMLRPRTRAAIAATRAAGIHVVLVTGRMFQS
                     VRRYALEAGIDDPVVCYQGAVVADPVSGRWLRHVPIPLELAREVIAALNAEGFGLNCY
                     VDDKLCVAEVSPEARRYADFQHIELHPVGNLLDWLDEPPTKLVVIDDPEVLDGLKQRM
                     VERFDGRLYISKSLPYFLEFASPDVTKATGLEFLAEHLGFARERTVAFGDGENDIELV
                     EWAGYGVAVENAHDLVKRVADFVCPSVDEEGVAQVLEAYLDSRP"
     gene            3143..4369
                     /gene="serS"
                     /locus_tag="VF225_07310"
     CDS             3143..4369
                     /gene="serS"
                     /locus_tag="VF225_07310"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7145103.1"
                     /translation="MIDLRAARDDPETFRAALARKGAADIFDELLEADRGVREIQPRV
                     EELRAARKVKGKPTPEQLEELERVKTELQSLEQQLAAAEGRRKELLDRVPNPPDEATP
                     DGETEDDAVQIRRWGDPRPFDFPVKDHLELAGPGIDLERGAKVSGSRFAFRSGDVALL
                     ELALYRFALDRLTSRGFTPVLPPVLVREEAMYGTGFFPTDEVNIYAVERDDLYLVGTS
                     EVPLAAMHGAEILDGDDLPLRYAGYSTCFRREAGAAGKDTRGMFRVHQFDKIEMFVFT
                     RPESSRDEHDRLLDIEEALVQELELPYRVVNVAAGDLGASATKKYDIEAWFPSQEHYR
                     EITSTSNTTDYQARRLEIRYRRDDKQLETVHTLNGTAVTGRAMLAMMENFQEADGSVS
                     IPEPLHSFGAPKKLNS"
     gene            4366..4542
                     /locus_tag="VF225_07315"
     CDS             4366..4542
                     /locus_tag="VF225_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HEX7145104.1"
                     /translation="MSWGYRKSKRLARGVRLNVRKRGVGVSVGPRHAKVSVNTSGRRT
                     VYLSLLGFFFRKRL"
     gene            4574..4662
                     /locus_tag="VF225_07320"
     tRNA            4574..4662
                     /locus_tag="VF225_07320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4608..4610,aa:Ser,seq:tga)
     gene            complement(4708..4932)
                     /locus_tag="VF225_07325"
     CDS             complement(4708..4932)
                     /locus_tag="VF225_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145105.1"
                     /translation="MDGPLELDARHAAGIDVELLWDPRTQGLSVVAHDATTDETVTIY
                     VEADQALEVFRHPFAYAGEPAPRVGLRVKA"
     gene            complement(5348..7564)
                     /locus_tag="VF225_07330"
     CDS             complement(5348..7564)
                     /locus_tag="VF225_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145106.1"
                     /translation="MIALVIVAAVTVSTWLATDAKGGLFALALLLATLVAVTAGRIWG
                     RAERERTRAESARSREQLRQAEAEYRAVTENLPLLTWLSAPGDRGSCAYISPQVEAML
                     GYSPAEWSAEPRLFSRLLHPDDRENVLEAHERADADGGRLRCQYRLVARDGRIVWVSE
                     ETATVRDAAGKALYTQTFLVDVSERKRGDDEREQLRAAERAAANLQTVRQRRLDFLRE
                     AGDILGATMDTQTSIQRVVDLAVHDLADWCAVDVLEASGELKRMGVAHAVPRRAEPSR
                     EPDGFVQAVIKSGRQLMIPLLAHQDSLDEEGAESGLPDEVVSLICVPLTSRGRPLGAL
                     TLARSAHGPEYGSDELTLAEDLAARIGIAIDRARLYLEVEERADAARVLTYVADAVLL
                     LDRADVVRLWNPAAESITGIASAAVLGRPPGEVIPGWRDAVDSIPISTSPSPGHPEVM
                     IPIEIEHGERWIAISGVKFFGGTVYAFRDVTEGRQLEELKADFIATASHELRTPLAAV
                     YGAAQTLLRHDFALDEAGRDRFVSLIAEESERLGRIVNEILLANQLDSGRVDLGTEPF
                     DAVELVERAVEAARAHAPAEISVEPVAHEPVPLVAADRDKVRQVIVNLIENAMKYSPD
                     GGRIEVGLQSGQSPDEETVIFYVKDEGIGIPSNELGRIFEKFYRLDPQMTRGVGGTGL
                     GLYICSELVHRMGGNIWVESSEGQGSTFLLELPVEAGPFPGALPVPAQSAGARTSG"
     gene            complement(7823..8929)
                     /locus_tag="VF225_07335"
     CDS             complement(7823..8929)
                     /locus_tag="VF225_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7145107.1"
                     /translation="METPQLYRDLWPGSRRRILVVDDDPSLRLLLRVTLAADEFEIEE
                     VASAEEAREVARFWRPAVVLLDVQLPGLDGLSFCRQLTAGRSDPPDVILLTGTATPAA
                     EAQKVGARAILHKPFSPLDLIALIDGLDATPTERLVSRSEGDAEQLLVYARDLSRIVE
                     VERSQRRLLQHAYRQTVAALADALEAKDPGTGLHAQRVQHYAVALTEMVEPRLLDDAS
                     LEYGFLLHDVGKIGVGDQILNKPGPLSAAERDLIELHPAIGVEILSGVGLLEGEGIGV
                     VGSHHERWDGGGYPGGLRGDGIPLGARIFALADALDAMTSDRPYRPALTWEKATDEIL
                     SHDGSQFDPKVVRAFCTRERQLHRIYEEFSVAAA"
     gene            complement(9025..>9431)
                     /locus_tag="VF225_07340"
     CDS             complement(9025..>9431)
                     /locus_tag="VF225_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX7145108.1"
                     /translation="KTCAGRGSVGSPDPECEHCRGTGRKRTVSNLEVANLLQIEPCPA
                     CIAERCSRCGGVGTVSAERRIRLLVPPGMQDGTQLRVGGDGNDAGAGSIPGDLLVRVK
                     VLAPPRDPRFVRYVALVLLFVAIATLALYVSR"
BASE COUNT         1557 a         3178 c         3153 g         1543 t
ORIGIN      
        1 gccctccacc tcttgcggct ccagcctctg ccagagaccg cgcgtgaccg ccaccgcagc
       61 gtgcttcggg ctccgccccg tcgcgaacgc attaggcacg tccgtgtcga tgatcgcgac
      121 ccgcggcttc ggtagatcgg ccatcgcgca gagccgctcg atcatgtcgt gcagctcggg
      181 agcctgatcg cgctcgacca ccttggcgcc cgaggcggcc agggcgagct tgtccgacgt
      241 gaagtactgg aagaaggcga tcccgacgac gatcaggatc atcgggatga gcccgacgct
      301 caggacgttg aagaggacga ccgcaaagac gacgtacaga agcccgagca gagaggccgt
      361 ggtgaatatt cgcaacgtga gaccggcgtc gcgaccgtaa gacctgcgct tcatcgagaa
      421 tcgctctcct tgaaggcgag ggttatacga ggaatggtag ccgtgggact cgcgactgcg
      481 ggattcctcg gcgtgagcgg gacggcgcag gcaaggccca aggtctacgt catcccatcc
      541 caaggcgtct tggtcacccg cgaatttggg gcaatcggcc tctacgtccc cggcgccggg
      601 gggacggtga gccgtgccgg agcgatcgcg gccatcagac ggggcaaggt cgagagcgcc
      661 gccctgggcg ggaagccgca cgggaagatc ctcgtcgaca tcgtcttcgg cgttccggat
      721 ctgcaggtga agaacgcggt cctcgtcgaa ctgccaccgc ccggagggca tccgaacacg
      781 aagcgctact ccgtcatgat ttttggcgcc ggctaccacg gcatcctcac gtcggagtcc
      841 acacggatcc gcggcctcgt ctcgatcgcc gatctcggcc cgacggctgt ggcactgcaa
      901 gaggggcgca cgccgcagat ccgggccgag cccgatcggg acgcgcgaga ggatctgcgt
      961 gcgctcgaca ctcgtctcac gcgcgtgcac cacgaccgcg gctgggtgtt cgtcaccgtc
     1021 gtgctgacga tccttgcgct gacgatcttc gcaccgcgcg cggcggggct cggaggcgcc
     1081 gctgccttcg gcgcgtcgtt gctcctttcc tgggccggcg cgacacgcct cggcctggtg
     1141 atcgcgacga tcgcggccct gacgatcgcg ctcgccgttg caggctcgtt tcgtcgcagc
     1201 atcgtcccgc cggtcgtggc ggggttcctg gtggccctga cggtcgtgct cgcggtcgat
     1261 cccgagctca actcgctggc cgtgctcggc gcgcgaccgg acggcggggg acgtttctac
     1321 gggatcggca accaggtcga gacattgctc ctggctcccg tgctcatggc cgcagcgatt
     1381 ggcggactgc gttggctcgt cccgctcgga gccgttgcgc tcgtggcgat cggttggagc
     1441 aaggcggggg cggacggcgg cggcgtgctc gtcttcgcgg cggcgctcgg ggttctggcg
     1501 ttgcgtctcc gcgggctcgc ggtcactccg cggcggcttg ctctcgtcgc ggtggcagtc
     1561 ctcgtcgtcg cggtcgcatt cgtcggcctc gacgctgccc tcggtggatc gagtcacgtc
     1621 acgcgagcgc ttggaagcgg ccccgactcg ctcctcggcg gtcttggaca tcgcctgcac
     1681 ctctcgtggg cggccgtgac cgaccgctgg tttcacggag tcatcttcct cagttgtctc
     1741 gctgcgctcg tgttgatggg cacgattcgg ccgcgacggg ctacggtcga cgcactgctc
     1801 gccgggctcg cggtgtcgct gctggtaaac gacacaccgg tcgacgtgat cggcctcggc
     1861 gcgcttggct gctgggctct cctgcgttgg gagtcagtag actcgcgccc gatgcgccgg
     1921 ggagtcttga tcgccgccgc tgccaccgcg gccgtgctcg cactcgcggg ctgcgggagc
     1981 gaaggcgtcg ttcgtgcgtt gcccagcacg gtcgtcggaa cggtgaagac cgaggcgccc
     2041 ggcaaggcga tcttcatcag ccagggttgc ggcgcctgcc atacctacaa gcccgcaggc
     2101 ccgggagcga acgggaagat cggccccgac ctggacaagc tgccccagta cgcgaaggcg
     2161 gcgaaccagc ctctcgcgac gttcgttcac gactccatcg tcgacccgaa caagtacgtc
     2221 gagaagggct atccgaaggg cgtcatgccg accacctaca agtccctgtc ggccaccgac
     2281 ctcaaggctc tggtcgactt cctgacgaag ccgcagggct gaacctcccg cagaacttcc
     2341 cgcacgagat cgacgccgtg gcgtgcgacc tcgatcgcac tctgatcggt gaggacatga
     2401 tgctgcggcc gcgcacccgg gcggcgatcg ccgctacgcg cgccgccggg atacatgtcg
     2461 tgctggtgac cgggcggatg ttccagtccg ttcgccgtta cgcgttggag gcgggaatcg
     2521 acgatccggt tgtttgctac cagggcgccg tggtcgcgga cccggtgagc ggtcgctggc
     2581 ttcgtcacgt accgatcccg ctcgagcttg cgcgcgaggt gatcgccgca ctgaacgcgg
     2641 aaggcttcgg gctgaactgt tacgtggacg acaagctgtg cgtcgccgag gtgtcccccg
     2701 aagcgaggcg ctacgcggat ttccagcaca tcgagctgca tcccgtcgga aatctgctcg
     2761 actggctcga cgagccgccg acgaagttgg tggtcatcga cgaccccgag gtgctggacg
     2821 gcctgaagca gcggatggtc gagcggttcg acggccgcct ctacatctcg aagtcactgc
     2881 catacttcct cgagttcgcg tccccggacg tgacgaaagc gaccggtctg gaattcctcg
     2941 ccgagcacct cggcttcgca cgcgaacgca cggtggcatt cggtgacggg gaaaacgaca
     3001 tcgagctcgt cgagtgggcg ggctacggcg tggctgtcga gaacgcccac gacctcgtga
     3061 agagggtcgc cgacttcgtc tgcccctccg tcgacgagga aggcgtggct caggtgctcg
     3121 aggcctacct agactcccgc ccatgatcga tctccgtgcc gcccgcgacg atccagagac
     3181 cttccgggcg gcgctggcgc ggaagggagc agccgacatc ttcgacgagc tgctcgaggc
     3241 cgatcgtggg gtcagggaga tccagccacg tgtcgaggag ctccgtgcgg cccgaaaggt
     3301 caaggggaag ccgacaccgg agcagctcga ggagctcgag cgagtgaaga cggagctgca
     3361 gtctctggag cagcagctcg cggcggccga aggccggcgc aaagagctgc tcgaccgggt
     3421 cccgaaccca ccggatgaag cgacgcccga cggagaaacg gaggacgacg ccgtgcagat
     3481 ccgccgctgg ggcgatccaa ggccgttcga ctttcccgtc aaggaccacc tggagctcgc
     3541 tgggccgggc atcgacctcg agcgtggcgc gaaggtctcc ggctcccgct tcgccttccg
     3601 cagcggcgat gtcgcgttgc tcgagctcgc gctctaccgg ttcgctctcg accggctcac
     3661 gagccgcggc ttcacccccg tcctcccccc cgtcctggtt cgtgaggagg cgatgtacgg
     3721 gaccgggttc ttccctaccg acgaggtgaa catctatgcg gtcgagcggg acgacctcta
     3781 cctcgtcgga acgtccgagg tcccgctcgc tgcaatgcac ggcgccgaga ttctcgacgg
     3841 cgacgacctc ccgctgcggt acgccggcta ctccacctgc ttccgccgcg aggccggggc
     3901 cgccgggaag gacacgcgtg gaatgttccg cgtccatcag ttcgacaaga tcgagatgtt
     3961 cgtgttcacg cgaccggagt cgtcacgaga cgagcacgac cgtctgctcg acatcgagga
     4021 ggcgctcgtg caagagctcg agctgccata ccgggtcgtc aacgtcgccg ccggcgacct
     4081 cggtgcatcg gcgacaaaga agtacgacat cgaggcgtgg ttcccgtcgc aggagcacta
     4141 tcgcgagatc acgtccacct cgaacacgac cgactaccag gcacgccgcc tcgagatccg
     4201 ttaccggcgc gacgacaaac agttggagac cgtgcacacc ctgaacggca cagccgtcac
     4261 gggacgcgcg atgctggcga tgatggagaa cttccaggaa gccgacggct cggtctcgat
     4321 cccggagccg ctgcactcgt tcggcgcacc gaagaagctc aactcgtgag ctggggctac
     4381 cgcaaatcga agcggctcgc gcgcggcgtt cgcctcaacg tccggaagcg gggcgtcggc
     4441 gtaagcgtcg ggccgcgcca cgcgaaggtg agcgtcaaca cgagcggccg gcgaaccgtc
     4501 tatctgtcgc tcctcggctt cttctttcgc aagcgcctct agaccgccga gagcggcgct
     4561 accgtgtgca aacggcgggg tgccggagcg gccgaacggg tcggtcttga aaaccgatga
     4621 ccgccgcaag gcggtccgtg ggttcaaatc ccacccccgc cgctcgtcgc ctatcgcgtt
     4681 ggcactgctg acgcggaaat ggaccggcta cgccttgacc cgcaagccca ctcgcggagc
     4741 gggctccccg gcataggcga aggggtggcg gaagacttcg agagcctggt ccgcctcgac
     4801 gtagatcgtc acggtctcgt cggtcgtcgc gtcgtgagcc accactgaga gcccctgagt
     4861 gcggggatcc cagaggagct ccacgtcgat ccccgcggca tggcgggcat cgagctctag
     4921 cgggccgtcc atgtattgac gatggcgcgt ttgcgcctgg gcgtcaccgg ccgatgggag
     4981 ggtccaccta gccctaaggt ccgtgggctc acgacactca actgaagtta ggccactcgg
     5041 cctattgctt ccagcagacg cctgtgcgtc acttgaatgc gtgcctgagg ccgaagctca
     5101 tctgtaccgt ctcgggtctc cgctcgcctc gatcgccggc atcgcccagg cgacgctgga
     5161 aaggaacgac cttgatgacg acgtcgcgac gcgtctacgc gccatccgag atctcgcact
     5221 agacgccctg gcgaagggag aacggggcga ttcagagcca ccggctcgac cagccccttg
     5281 aggttggtct caggacggcg ggaatagatc gcaatacgga gcgcccaggc gcctctacac
     5341 gtgaggctca accagaggtg cgggccccgg cgctttgcgc ggggaccggc agagcgcccg
     5401 ggaacggtcc ggcttccact gggagctcga gcaggaaggt tgatccctgt ccctcgctcg
     5461 attcgaccca gatgtttccc cccatgcggt ggacgagctc gctgcagatg tagaggccga
     5521 gcccggtccc gccgacgccc cgcgtcatct gcggatcgag gcgatagaac ttctcgaaga
     5581 ttcggcccag ctcgttcgac gggatgccga tgccttcgtc cttcacgtag aagatcaccg
     5641 tctcttcatc cggcgactgt cccgactgta gtccgacctc aatccggccg ccgtcgggcg
     5701 agtacttcat cgcattctcg atcaggttca cgatgacttg ccggaccttg tcgcggtcgg
     5761 ccgcgacaag gggcacaggc tcgtgagcga ccggttcgac cgagatctcc gccggcgcat
     5821 gcgcacgtgc ggcctcgacg gcccgctcga ccagttcgac cgcatcaaac ggctctgtgc
     5881 cgaggtcgac ccgtcctgag tcgagctggt tcgcgagcag aatctcgttc acgatccggc
     5941 ccaggcgctc ggattcttcc gcgatcagcg agacgaaccg gtcgcggcct gcctcgtcaa
     6001 gcgcgaaatc gtgacggaga agcgtctgcg cggctccata gacagcggcg agcggcgtcc
     6061 gcagctcatg ggaggccgtg gcgatgaagt cggccttcaa ctcctcgagt tggcggccct
     6121 ccgttacgtc gcggaaggca tagaccgtcc cgccgaagaa cttgacgccc gagatcgcga
     6181 tccaccgctc tccgtgctcg atctcgatcg ggatcatcac ttcggggtgg ccgggagagg
     6241 gtgaggtcga gatggggatc gagtcgacgg cgtcgcgcca gccgggtatc acctctcccg
     6301 gaggccgtcc caggacggcg gccgaggcaa ttcccgtgat gctctcggca gccgggttcc
     6361 agagccggac gacgtcggcc cggtcgagga ggaggacggc gtcggcaacg tatgtgagga
     6421 cgcgggcggc gtcggcacgc tcctcgacct cgaggtagag acgcgcgcga tcgatcgcga
     6481 tgccgatccg cgccgcgaga tcctcggcga gcgtgagctc gtccgaaccg tactccggcc
     6541 cgtgggcgct acgtgcgagc gtgagggcac cgagcggccg gcctcgactc gtgagtggca
     6601 cgcagatcag cgagacgacc tcatcgggaa gtccagactc tgctccctcc tcatcgaggg
     6661 agtcctgatg cgcgagcagc gggatcatca gttggcgtcc cgacttgatc acggcttgca
     6721 cgaagccgtc gggctcccgg gacggctctg cacgccgcgg taccgcgtgt gcaactccca
     6781 tccgcttcag ctccccgctt gcctcgagca cgtcgaccgc acaccagtcg gcgagatcgt
     6841 gaaccgccaa gtcgacaacg cgctggatcg acgtctgcgt gtccatcgtg gcgccgagga
     6901 tgtcgccggc ctcgcgcagg aaatcgagcc gacgctgccg gacagtttgc agattggccg
     6961 cagcgcgctc cgcggcgcgc aattgttctc gctcgtcgtc ccctcgcttg cgctcactga
     7021 cgtccaccag aaacgtctgg gtgtagagcg ccttccccgc ggcatcccgg accgtcgcgg
     7081 tctcctcact gacccagacg attcgcccat cgcgggcaac aagccggtac tggcaacgaa
     7141 gccggccgcc gtcagcgtcc gcacgctcgt gtgcttcgag cacgttctcg cggtcgtcgg
     7201 gatgcaggag ccgggagaag agccgtggct ccgcactcca ctctgcaggc gagtaaccga
     7261 gcatcgcctc cacttgtggg ctgatgtacg cgcaagagcc ccggtcaccg ggcgcagaca
     7321 gccacgtcaa aagggggaga ttctcggtga cggctcggta ctccgcctcc gcctgccgga
     7381 gctgctcgcg cgatcgtgcc gactcggcgc gagtgcgctc gcgttcggct cgaccccata
     7441 ttcgccccgc cgtcacggcg acgagcgttg cgagtaggag cgcgagcgcg aacaacccgc
     7501 ccttcgcatc ggttgccaac cacgtcgaga cagtcaccgc ggcgacgatc acgagcgcga
     7561 tcacagcggc gaggatgaga cgaagtggag accgggccgg tccggacaca ggcgacgtgc
     7621 gtgagcctag ctccgcggtc gcgatgtcac aaggcgattc ggtgaattcg gacagtgctc
     7681 gctcccaacg gcgggcctcg ttcctcgaag ggacgttatt tcgccccgaa aggccgtttt
     7741 ccttcctcta tgggaaaaca ctcaagtcca gcacgagggt gtctcagcgc gtcggcgccg
     7801 gtcctctccc cataggcacc gctcaggccg cggcgaccga aaactcttcg taaatgcggt
     7861 gaagctgtcg ctcgcgtgta caaaaagcac gaacgacttt cggatcgaac tgagagccgt
     7921 catgcgacag aatctcgtcg gtcgccttct cccaggtgag cgccggtcga tacggacggt
     7981 cgctcgtcat cgcgtcgagc gcgtcggcca gggcgaagat ccgcgcgccg agcgggatcc
     8041 cgtcgcccct caggccaccg ggatagccgc cgccgtccca gcgttcgtgg tgcgacccca
     8101 ccaccccaat cccttcgccc tcgagcagcc cgacgccgga caggatctcg acgccgatcg
     8161 cggggtgcag ctcgatcagg tcgcgctcgg ctgcactcag gggcccgggc ttgttgagga
     8221 tctgatcgcc gacgccgatc ttgccgacgt catggaggag aaacccgtac tcgaggctcg
     8281 catcgtcgag caggcgaggc tcgaccattt cagtgagggc gaccgcgtag tgctggacgc
     8341 gttgcgcgtg gagacccgtt ccgggatctt tcgcctcgag cgcgtccgcg agcgcggcca
     8401 cggtctggcg gtacgcgtgc tgcagcagcc gacgctggga acgctcgacc tcgacgatgc
     8461 ggctcaggtc gcgtgcgtag accagaagtt gctcggcgtc tccttcgctg cgtgagacga
     8521 gacgctcggt cggcgtcgcg tcgagcccgt cgatcagcgc gatcagatcc agcggactga
     8581 acggcttgtg caggatcgcg cgcgctccga ccttctgcgc ctctgcggcc ggcgttgctg
     8641 ttccggtcag caagatgacg tcgggtggat cgctgcgtcc cgcggtgagc tgccggcaga
     8701 acgaaagccc gtcgaggccg ggaagctgca cgtcgaggag cacaaccgcc ggccgccaga
     8761 atcgcgcgac ctcccgcgct tcttcggccg acgcaacctc ctcgatctcg aactcgtccg
     8821 cggcgagcgt cacgcggagc agcaggcgca aactgggatc gtcgtccacg acgaggatcc
     8881 ggcgtcggct gccgggccaa agatctcggt agagctgcgg cgtctccatt agggggtaga
     8941 gcggcggccg cagcaggcac cgttccaggc aaagcctagc gccaaaaacg ccgatttggg
     9001 gcgaaatccc tcaactgagc gggctcagcg cgatacgtag agcgcgagcg tcgcgatcgc
     9061 gacaaagagc agcacgagcg ccacgtagcg gacgaaccgc ggatcccgcg ggggcgcgag
     9121 gaccttgacg cgcacaagta ggtcgcctgg gatggagccg gcgcccgcgt cgttgccatc
     9181 accgccgacc cgcagctgcg tgccgtcctg cattcccggg ggcacgagca ggcggatcct
     9241 ccgttcggcc gagaccgtcc ctactccgcc gcagcgcgag cagcgctccg cgatgcaggc
     9301 cgggcacggc tcgatctgca agaggttcgc cacttcaagg ttcgacaccg tgcgcttgcg
     9361 tcccgtgccg cggcagtgct cgcattcggg gtcgggcgaa ccgaccgagc cccgccccgc
     9421 gcaggtcttg c
//
LOCUS       DASVEU010000179         9232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1679272,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000179 DASVEU010000000
VERSION     DASVEU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679272"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF225_07345"
     CDS             complement(<1..224)
                     /locus_tag="VF225_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145109.1"
                     /translation="MSVWDDDRHGWGAGPKRALQLALAEGRLLARRPRPDVDAFIVGY
                     PGHFDLPAARRAAHGRPLIFNPLVSLADTLV"
     gene            complement(323..1261)
                     /locus_tag="VF225_07350"
     CDS             complement(323..1261)
                     /locus_tag="VF225_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7145110.1"
                     /translation="MISVVVPVHDEERSVALLYDELRSAIEPLHEPWEVIFVDDGSTD
                     GTFSALTRLHNSEQNVRVVRLRRNFGKATALVAGFDQVRGETVVTIDGDLQDDPAEIP
                     RLLAKLDEGFDLVTGWKTHRRDPFSRRLLSRLFNRVTSAFSGVRLHDMNCGLKAYRKE
                     VVHGLRLYGELHRFIPVLAHYRGFRIAELPVNHRPREHGRSRYGVERYLRGFLDLLTV
                     SFIGRYGHRPLHLFGGLGLLLGAIGSAILIYLTVVKALGHAIGQRPLLLLGVLLVVIG
                     MQFFSLGLISEMITSHHEERVQERDRAELLVDEVLS"
     gene            1290..2483
                     /gene="glp"
                     /locus_tag="VF225_07355"
     CDS             1290..2483
                     /gene="glp"
                     /locus_tag="VF225_07355"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7145111.1"
                     /translation="MAELLSLEEALKRILERVTPLEPEAVPLAAAAGRVLAEDARAVV
                     DLPPFPSSAMDGFAVRSEDTPGRLPVVARIAAGRPAPRPLELGEAMAIATGGVVPAGA
                     DSVIPLEYVVDHDNEVEIPTAVVQGDNVRPRGGDVAAGDVVVPHGARLRAAQIGALAA
                     AGLDGVVVARRPRVAVLATGTELRRPGETLGPGEVYEANGVLLATALASAGADVDTLP
                     AVADDESSHRDALERGLAADVLVTSGGVSVGPHDLVRRILAELGVEEVFWGVAVKPGK
                     PLAFGVRGSTLVFGLPGNPVSSLVGAEVFVRPALLALQGAKVPGPVFFEGRLAAAVRR
                     NSHRDEFVRATSAASENGVLLYPVRGQESHMIARAAAADALVLAPRGEGELDAGESVR
                     YLPLA"
     gene            complement(2465..3163)
                     /locus_tag="VF225_07360"
     CDS             complement(2465..3163)
                     /locus_tag="VF225_07360"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX7145112.1"
                     /translation="MRRRIRLLGTVLATAGVLTLLWALLVWQWQDPFTAIYTKWKQHQ
                     LASQYDKRTRSFKPAISGVTQAAEQRSIAREAKQYRRSSRRGEVIGRLRVPRMGVNML
                     LVNGTDHDTLKKGPGRDERTFMPGENRLVYIAGHRTTYLAPFSHIDRLRSGDRVTIEV
                     PYGTFIYAVTRHRIVKSTDLSVLRSPRHEVVELQACHPRFFATHRYIAYARLLRVEPR
                     GAKPYELAAQASGK"
     gene            complement(3164..5044)
                     /locus_tag="VF225_07365"
     CDS             complement(3164..5044)
                     /locus_tag="VF225_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7145113.1"
                     /translation="MASVPPRTERRRARPGSLERPVNGRLYRGTWLLVGLPLLVAAFS
                     VARPTPMPRAFLPAFDGRATKTLAQDLADRPYRQPGSPQALAAAGWFRDQLTPYGLPI
                     RTERFSAVIPGRGKVELQNLVAEASGRSPSTIVVMAHRDNDGRGPGANDNASGTAMLI
                     QLARAYGVPPGRPAAQLRPNHTILFVSTDGGAYGGVGAAWFAAHDPLRNDVAGVINLD
                     SVGGNGRIRMEFSGDTPREPSGIFLQTVAARIAAQTGRAPARPGAVRQLIDLGFPFSL
                     YEQAPFLAHGIAAVTLTTAGDNPPDPVSDTGDRLRADKLAQVGRAAQDALGTLDEGLE
                     FTQGTSTYLYLGSRLIRGWAIELVLIACLLPFLATAVDLFARCRRRHIALAPALRAYR
                     SRLAFWAWVVGLFELFRLLGAWPNGVARPIPPSSALAHDWATKGLLGLAVLAVLGWFV
                     TRERLIPRRAITTEDELAGHTASLLCLGALSLLVVATNPFALLFLLPSLHVWLWLPQV
                     RGAPLGARLGVLTLGFAGPALLIGSFAGRFGLGWDAPWYLAELRSVGYVPFVVMPLLV
                     VWLAGAGQLAALAARRYAPYPSAAELPPSGPLRRLVRSTVLAVHDRRRRSSSTAPEAL
                     EG"
     gene            5114..5812
                     /locus_tag="VF225_07370"
     CDS             5114..5812
                     /locus_tag="VF225_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145114.1"
                     /translation="MPDSSTILLVDDEDSIQKLLTYPLEREGYRVLQARDGEEALTRF
                     ASERVDLVVLDIMLPKLDGLEVCKRLRAESEVPIIMLTARDDELDKVLGLELGADDYI
                     TKPFSIREFRSRVRALLRRASVVRQVNEGDELITAEGLTIDLSRRVVEVGGERVQLTY
                     VEFELLRILASHPGRVYSRRMLLEALWGGADYREPRTIDVHVRHLREKLERDPAEPGY
                     ILTVRGVGYRFRDR"
     gene            5809..7242
                     /locus_tag="VF225_07375"
     CDS             5809..7242
                     /locus_tag="VF225_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145115.1"
                     /translation="MNPLHSVGARLGLGLAVVVAAALVLVDLIVVPSLERNLIDAKLT
                     QLEAAAPTVGSSLLLSPSYARDDAIQAAHQSSDARVVYFRPLTYNPPKLLVNGDSSPV
                     GSADVENDPFALRAFAQARSARGTVTTHGIRYAEAAYPLIDGSVVLLRASLHDSLRSI
                     HLVRRRLVVAGLIALAASLLVGYAAASVFARRIRRLERAADRIASGDFSEPVVDSGRD
                     ELAQLAAAFERMRLRLAQLEHARREFIANASHELRTPLFSLGGFLELMTEEELDPKTQ
                     AEFMATMREQVERLTKLATELLDLSRLDAGRLSVEREPLDLTTLAETLADEFRAVARS
                     TEHQLSVRAAGPIEGIGDDERALQIGRILVENALVHTPPGTAVRLGTGRRDGLALLTV
                     EDEGPGIPAEHEGQIFDRFYRIEGSVASGSGLGLAIARELAELMGGSVELDVSDGRTR
                     FALVLPVSAEAVPFSPENERVEEPSLQ"
     gene            7248..8477
                     /locus_tag="VF225_07380"
     CDS             7248..8477
                     /locus_tag="VF225_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7145116.1"
                     /translation="MRLSALAVVTVAAAALGGGAALGIGKGAGWLDQGTKTVVVRAAA
                     PRTAALPASATSTAAPLPGKGFDPQRIFAERSPGVVTIFAYFGDPSSTATAVSQGSGF
                     VISPKGYVLTNSHVITNAGEGSSVRAAQHLFVEFADGDRAEAKVVGWDLYDDVGLLQL
                     KTNAHPLSPVPLGDSATVAVGEPVAAIGSPLGSDIGVNTLTVGVVSAIHRSIDALTVK
                     LYKVVDAIQTDAPITHGNSGGPLLDARGRVIGINAQIRSANGTGTNDSGIGFAIPIDA
                     AKHSVAQLISKGKVTYAYVGISTEDLTPSLARALGYKADRGALIVRVTGGSPGDKAGL
                     HGGNREADVLGRTLETGGDVILAIDGKPVDGADDVVRFVSFSLRPKDVAVFTILKPGG
                     ERKKVAVTLTERRLPAG"
     gene            8597..9028
                     /locus_tag="VF225_07385"
     CDS             8597..9028
                     /locus_tag="VF225_07385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145117.1"
                     /translation="MTTSTNSGAVTVRLTIPARAEYITLCRLALTGIGRLRELSDELL
                     ADLKLALTEAASNSVRHAYGETRDVGVVEISYELHSDRLVIEVTDEGEGFDPAAAAGH
                     SDELSEGGLGIAIIRAIADEVEIGVQPGGKGSRLRFAKALI"
BASE COUNT         1469 a         3083 c         3228 g         1452 t
ORIGIN      
        1 accagggtgt cggcgagcga cacgagcgga ttgaagatca gcgggcggcc gtgcgctgcg
       61 cgccgtgccg cgggcaggtc gaagtggccg ggataaccca cgatgaacgc gtcgacatcc
      121 ggccgcggcc ggcgtgcgag cagccgcccc tccgcgaggg cgagctgcag tgcacgcttc
      181 ggccccgctc cccagccgtg acgatcgtcg tcccacacgg acacgtgctc ctcgagaacc
      241 tctacgccag cgccgcgcag gcacgagatc acctgcgagt tacgcggata gccgcgctcg
      301 taagttccga aataacagag tttcaggaca ggacttcgtc gacgagcagc tctgcgcggt
      361 cgcgctcctg tacgcgctcc tcgtggtggc tcgtgatcat ctcgctgatc agtccgagcg
      421 agaagaactg cattccgatc accacgagca acacgccgag gagcagcaat ggacgctgtc
      481 cgatcgcatg gccgagtgcc ttgacgaccg tgaggtagat gaggatcgcg gagccgatcg
      541 ccccgagcaa cagcccgagc ccgccgaaca ggtgcagggg acggtggccg tagcggccga
      601 tgaaagagac cgtcagcagg tcgaggaacc cgcgcaggta acgctcgacc ccgtagcgcg
      661 agcggccgtg ctcccggggt cgatggttga ccggcagctc ggcgatgcgg aagccgcggt
      721 agtgcgccag caccgggatg aaccggtgca gctccccata gagacgcagt ccgtggacga
      781 cctccttgcg gtaggccttg aggccgcagt tcatgtcgtg caggcgcacg ccggagaacg
      841 cgctcgtgac gcggttgaag aggcgtgaaa ggagccgccg cgagaaggga tcgcgtcggt
      901 gcgtcttcca gccggtgacg agatcgaagc cctcgtcgag cttcgcgagc agccgtggaa
      961 tctcggcggg atcgtcctgc aggtcgccgt cgatcgtgac gacggtctcg ccgcgcacct
     1021 ggtcgaaccc ggccacgagt gccgtggctt tcccgaagtt gcggcgcagg cgaacgacgc
     1081 gcacgttttg ctcggagttg tgtagccgtg tcaatgccga gaacgtcccg tcggtcgagc
     1141 cgtcgtcgac gaagatcacc tcccatggct cgtgcagcgg ctcgattgcg gaccggagct
     1201 cgtcgtagag cagcgcgaca cttcgctctt cgtcatgcac cggtacgaca acggagatca
     1261 cgcgcgcgag gttataccct ggacgaggaa tggctgagct gctctcgctc gaggaggcgc
     1321 tgaagcgcat tctcgagcgg gtgacgccgc tcgagcccga ggccgtgccg ctggcggccg
     1381 cggcgggtcg cgtccttgcc gaggatgcgc gcgccgtcgt cgatctgcct ccgtttccca
     1441 gctcggcgat ggacggcttc gcggtgcgtt ccgaggacac gccgggacgc ctgccggtcg
     1501 tcgcgcgcat cgccgccggc cggccggcac cgcggccgct cgagctgggt gaggcgatgg
     1561 cgatcgccac cggtggcgtt gtcccggcag gcgcggactc cgtcatccct ctcgagtatg
     1621 ttgtcgatca tgacaacgaa gtggagatcc cgacggccgt cgtccaggga gacaacgtcc
     1681 ggccgcgtgg cggcgacgtt gccgcaggtg acgtggtggt tccgcacggc gctcggctgc
     1741 gcgccgcgca gatcggggcg cttgccgccg caggcctcga cggcgttgtt gtcgctcggc
     1801 gccccagggt cgcagtgctt gcgactggca cggaactgcg cagacccggc gagacccttg
     1861 gccccggcga ggtgtacgag gcgaacggcg tgctgctggc gacagcgctc gcctccgcgg
     1921 gcgccgacgt cgacacattg ccggccgtag ccgacgacga gtcgtcacat cgcgacgcgc
     1981 tcgagcgggg cctcgcggcc gacgtgctcg tcacttcggg cggtgtctcc gtcggaccgc
     2041 acgacctcgt ccgccggatc ctggccgagc tcggggtcga ggaggtcttc tggggtgtcg
     2101 cggtcaagcc cggtaagccg cttgcattcg gcgtgcgcgg gtcgacgctc gtcttcggac
     2161 ttcccggcaa tccggtctcc tcgctcgtcg gagccgaggt gttcgtgcgg cctgctctgc
     2221 ttgccctgca gggtgcaaag gtccctggtc cggtgttctt cgagggccgg ctcgcagcag
     2281 cagttcggcg caactctcac cgcgacgagt tcgtgcgagc gacatccgcc gcgtccgaga
     2341 acggcgtcct cctctatccg gtccgcgggc aggagtcgca catgatcgct agggccgccg
     2401 ccgccgatgc gcttgtgctc gcaccccgag gcgaaggcga gctcgacgcc ggtgagagcg
     2461 tgcgctactt gccgctcgcc tgagcggcca gctcgtacgg tttcgctccg cgcggctcga
     2521 cgcgcagtaa ccgcgcgtag gcgatgtacc ggtgtgtcgc gaagaagcga ggatggcacg
     2581 cctgcagctc gacgacctcg tggcgggggg agcggagcac cgagaggtcc gtcgacttca
     2641 cgatgcgatg tcgcgtcacg gcatagatga aggtcccgta cgggacctcg atcgtgacgc
     2701 ggtcgccgct ccgaagccgg tcgatgtgcg aaaacggcgc caggtacgtc gtgcggtgcc
     2761 cggcgatgta gaccagccgg ttctctcccg gcatgaacgt ccgctcgtca cgcccaggcc
     2821 ccttcttgag cgtgtcgtgg tcggtgccgt tgaccagcaa catgttcacg cccatccgag
     2881 ggacgcgcag gcgtccgatc acttcgcctc gtctcgagct cctgcggtac tgcttggcct
     2941 cgcgcgcgat gctccgctgc tctgccgcct gcgtgacgcc cgagatcgcc ggcttgaacg
     3001 atcgcgtccg cttgtcgtat tgcgaggcga gctgatgctg cttccacttc gtgtagatcg
     3061 cggtgaaggg atcctgccac tgccatacga gcagagccca gagcagcgtg agcacgcctg
     3121 cggtggcgag caccgttcca agcaggcgga tgcggcgacg catctagccc tccaacgctt
     3181 ccggggccgt cgaggatgag cgtctgcgcc ggtcgtggac cgcgagcacg gtgctacgca
     3241 ccagtctgcg cagcggaccg ctcggcggaa gctcggcggc cgacgggtat ggggcgtagc
     3301 gtcgcgccgc cagcgcggcg agctgtcccg ctcctgcaag ccagacgacc agaagcggca
     3361 tgacgacgaa cggtacgtaa ccaaccgatc tgagctctgc gagataccag ggggcatccc
     3421 agccgagccc gaaccgtccg gcgaaggagc cgatcaacag cgccgggccg gcgaagccca
     3481 gggtgagcac tccgagccgg gcgccgagcg gcgcgccgcg cacctgtggg agccagagcc
     3541 agacgtggag cgacggcagc aggaacagga gcgcgaacgg gttcgtcgcg acgaccagca
     3601 gtgagagtgc gccgaggcag aggagcgacg ccgtgtgtcc ggcgagctcg tcctcggtcg
     3661 tgatcgctcg ccgcgggatc aaccgttcac gtgtgacgaa ccagcccagg actgcgagca
     3721 ccgcgaggcc gagcagcccc ttagtcgccc agtcgtgcgc aagcgcgctc gagggcggaa
     3781 tcggacgagc gacgccgttc ggccacgcgc ccaacaacct gaacagctcg aagagaccca
     3841 ccacccacgc ccagaacgcg aggcggctgc ggtacgcacg cagggccggg gcgagtgcga
     3901 tgtgcctgcg ccggcagcgg gcgaacaggt cgaccgctgt tgccaagaaa ggcaacaaac
     3961 aggcgatgag gacgagctcg atcgcccagc cgcggatgag gcgcgagccg agatagaggt
     4021 acgtggacgt tccctgcgtg aactcgagac cctcgtcgag ggtcccgagc gcgtcctgcg
     4081 ctgcgcggcc gacctgcgcc agcttgtctg cgcgcagccg gtctccggtg tcgctgacgg
     4141 gatcgggcgg gttgtcgccc gcggtcgtga gcgtgacggc cgctatcccg tgcgcgagaa
     4201 acggcgcctg ctcgtagagc gagaaaggga agcccaggtc gatcagttgg cgtactgcgc
     4261 ccggacgcgc cggcgctcgc cccgtctggg cggcgatgcg cgcggcaacc gtctgcagaa
     4321 agattccgga cggctcgcga ggagtgtcac cgctgaactc catgcggatc cgtccgttgc
     4381 cgccgacgga atcgagattg atgacgcccg cgacgtcgtt gcgaaggggg tcatgagctg
     4441 cgaaccaggc tgcaccgacc ccgccgtacg cgcctccatc ggtcgagacg aagaggatcg
     4501 tgtggttcgg tcgaagctgg gccgccggcc ggccaggtgg gacgccgtac gcgcgcgcga
     4561 gctgaatcaa catggccgtc cctgacgcgt tgtcgttcgc accaggaccg cgcccgtcgt
     4621 tgtcgcggtg tgccatgacg acgatggtgc tcggcgagcg gccactcgcc tctgctacga
     4681 ggttctgcag ttcgactttg cctcgcccgg gaatgacggc gctgaagcgc tcggttcgga
     4741 tcggcaatcc gtagggcgtg agctggtcgc ggaaccagcc cgccgcggcg agcgcctgcg
     4801 gggagcctgg ctgacggtac ggccggtcgg ccaggtcctg cgcgagcgtc ttggtcgccc
     4861 tgccgtcgaa cgccggcagg aacgcgcggg gcatcggagt cggtcgcgcg acgctgaagg
     4921 ctgccacgag gagcggcagt cccacgagca gccaggtgcc tcggtacaga cgtccgttga
     4981 ccggtcgctc cagagagccg ggacgggccc ggcgccgctc ggtccggggc ggaacggagg
     5041 ccatccaggg ctatagtagg cgccgaacga cctggcaagg atcgtccggc aggcactgtt
     5101 agccgagcac gacatgcccg actcctcaac catcctcctc gtcgacgatg aggactccat
     5161 ccaaaagctc ctcacttacc ccttggagcg cgagggctat cgcgtgctgc aagcgcgtga
     5221 cggtgaggaa gcgctgacgc gcttcgcatc cgagcgagtc gacctcgtcg tgctcgacat
     5281 catgctcccg aagctcgacg ggctcgaggt gtgcaagcgt ctgcgcgccg agagcgaggt
     5341 gccgatcatc atgctgaccg ctcgcgacga cgagctggac aaggtgctcg ggctcgagct
     5401 cggcgccgac gactacatca cgaagccctt ttcgatccga gagtttcgga gccgcgtccg
     5461 tgcactcctg cggcgggcgt ccgtcgtgcg gcaggtcaac gagggcgacg agttgatcac
     5521 agccgaagga ttgacgatcg atctctcccg tcgggtggtc gaggtcgggg gcgagcgggt
     5581 gcagctcacc tacgtcgagt tcgagctcct gcggatcctc gcctcccatc cgggtcgcgt
     5641 ctacagccga cggatgctcc tcgaggcgct ctggggcgga gccgattatc gcgagccgag
     5701 gacgatcgac gtccacgtca ggcatctgcg cgagaagctc gagcgggatc cggccgagcc
     5761 cggctacatc ctgaccgtgc ggggcgtcgg ctaccgcttc cgcgaccggt gaaccccctc
     5821 cacagcgtcg gtgcccgcct gggcctcggg ttggcggtgg tggtggctgc tgcgctggtg
     5881 ctggtcgacc tgatcgtggt gccgtccctc gaaaggaacc tcatcgatgc caagctgacg
     5941 cagctcgagg cggcggcgcc cacggtgggc tccagcctcc tgctcagccc gagctacgcg
     6001 cgcgacgacg ccatccaggc ggcccaccag agctcggacg ccagagtcgt ctacttcagg
     6061 ccgctcacct acaacccgcc gaaactgctc gtcaacggcg actccagtcc ggtggggtcg
     6121 gcggacgtcg agaacgaccc ctttgcgcta cgtgccttcg ctcaggctcg tagtgcgagg
     6181 ggcacggtca cgacgcacgg catcaggtac gcggaggcgg cctatccgct catcgacggt
     6241 tccgtcgtcc tgctgcgcgc ctcgctccac gactccctcc ggagcatcca tctcgttcgc
     6301 aggcgactcg tcgtggcggg tctgatcgcc ctcgccgctt ccctcctcgt cggctacgcg
     6361 gcggcgtcgg tcttcgcccg gcggatccgc cggctggagc gcgccgccga ccggatcgcg
     6421 agcggcgatt tcagcgagcc ggtcgtcgac tcgggccggg acgagcttgc ccagctcgcc
     6481 gcggcattcg agaggatgcg gctgcggctg gcgcagctgg agcacgcccg gcgggagttc
     6541 atcgccaacg cctcccacga actgcggacg ccgctcttct ccctcggcgg ctttctcgag
     6601 ttgatgaccg aggaggagct cgacccgaag acgcaggccg aattcatggc cacgatgcgg
     6661 gagcaggtcg agcggctgac gaagctcgcg accgagctcc tcgatctctc gcgtctggat
     6721 gcggggcggc tcagcgtcga acgcgagccg ctcgatctca ccacgctggc cgagacgctg
     6781 gcggacgagt tccgcgcggt ggcccgttcg accgagcatc agctctcggt gcgtgccgcc
     6841 gggccgatcg agggaattgg ggacgacgag cgcgcgctgc agatcggtcg aattctggtg
     6901 gagaacgcgc tcgtccacac gccgcccggc acggcggtcc ggctcggaac gggccgccgg
     6961 gacggcctgg ccctgctgac cgtcgaggac gaaggcccgg ggatcccggc ggagcacgag
     7021 ggccagatct tcgaccgctt ctatcgcatc gagggctcgg tcgcgtccgg cagcggcctc
     7081 gggctggcca tcgcaagaga gctggccgag ctgatgggcg gctcggtcga gctcgacgtg
     7141 agcgacggcc gcacgcgatt cgccctcgtc ttgccggtct ccgcggaggc ggtgccgttt
     7201 tcaccggaaa acgaacgcgt ggaagaaccg tcgctacagt gagccggatg cggctctcgg
     7261 ctctcgccgt cgtgaccgtc gctgccgccg ccctgggcgg cggcgctgct ttgggcatcg
     7321 gtaaaggcgc gggctggctc gaccaaggca ccaagacggt cgtcgtacgt gcggcagctc
     7381 cccggactgc ggccctgccg gcttctgcca cgtcgacggc agcccccttg cccggcaagg
     7441 ggttcgatcc gcagcgaatc ttcgccgagc gttcgccggg ggtggtcacg atcttcgcct
     7501 atttcggcga tcccagctcg acggcgaccg ctgtgtccca ggggtccggg ttcgtcatct
     7561 cacccaaggg ctacgtcctc accaattcgc acgtgatcac gaacgccggg gagggatcga
     7621 gcgttcgggc ggctcagcat ctcttcgtcg agttcgccga cggggaccgg gccgaggcga
     7681 aggtcgtcgg ctgggatctc tacgacgacg tcggcctcct gcagctgaag acgaatgcgc
     7741 atccgctctc gcccgtcccg ctcggagact cggcgacggt ggccgtcggc gagcccgtgg
     7801 cggcaatcgg cagcccgttg ggcagcgaca tcggcgtcaa cacgctgacc gtgggagtcg
     7861 tgtcggcgat ccatcgctcg atcgacgcgc tcacggtcaa gctgtacaag gtcgtcgacg
     7921 cgatccagac cgacgcaccg atcacgcacg gaaactccgg cggtcctctc ctcgacgcgc
     7981 gcggccgcgt aatcgggatc aatgcgcaga tccgtagcgc gaacggaacg ggcactaatg
     8041 acagcgggat cgggttcgcc atcccgatcg acgctgcgaa gcattcggtc gcgcagctga
     8101 tctccaaggg caaggtgacg tacgcctatg tgggcatcag caccgaggac ctgacgccgt
     8161 cgctggcccg ggcgctcgga tacaaggccg atcgcggcgc actgatcgtc cgcgtcacgg
     8221 gcggcagccc gggggacaag gccgggctcc acgggggtaa tcgggaggca gacgttctcg
     8281 gccgaacgct cgagacgggg ggcgacgtga ttctggcgat cgacgggaag cccgtcgacg
     8341 gcgccgacga tgtcgtgcgg ttcgtctcct tctcgctgag gcccaaggat gtggcggtgt
     8401 tcacgatcct gaagcccggg ggcgagcgca agaaggtggc cgtgacgctg acggagcgcc
     8461 ggctgccggc gggttagtcc tggtggggcc tgggcataaa accgtttgag agcaagtggc
     8521 ttgagagctc tcgacaagtt tcgacaggtt tattttgcgt ctttctgggc aagactcctt
     8581 tcgtgcaggg cactcgatga caaccagcac caactcaggc gccgtgacgg ttcggctgac
     8641 gattcccgcg cgagccgagt acatcaccct ctgccggctc gctctcaccg gcatcgggcg
     8701 gctgcgtgag ctgtccgacg agctcctcgc ggatctcaag ctggcgctga ccgaggccgc
     8761 atcgaactcc gtccggcacg cgtacggaga aacccgggac gtcggtgtgg tcgagatctc
     8821 ctacgagctc cactccgacc ggctcgtcat cgaggtcacc gacgagggtg agggattcga
     8881 tccggcagcc gccgcgggcc attccgacga gctctccgaa ggaggtctcg gcatcgcgat
     8941 catccgtgcg atcgcggacg aggtcgagat cggcgttcag cccggcggca aagggtcgcg
     9001 cctgcgtttc gcgaaagcct tgatttagcg gctccagccc gctcggcctg tattctcgcg
     9061 accgttcgtc ccccaggaac gcgcccagac catgctccaa ctcgtcaacg ccggacacaa
     9121 gtcgctcgcc gactacgcga cgatcgcgac gcgtgggctc atggacgaga tcaagcggct
     9181 ggccgagccg ctcgccggga agcgcgtcgt gcacctgtcg gccacggcct tc
//
LOCUS       DASVEU010000180         8039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_901336,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000180 DASVEU010000000
VERSION     DASVEU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901336"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VF225_07390"
     CDS             <1..1393
                     /locus_tag="VF225_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX7145118.1"
                     /translation="DLSLERAQLGSCRNGTYVPASIAEGEAFWRIVGLYIAEGHCTAD
                     GKRRRLQWSFHPRDEFDLVEEVYSFWAAQRVRASVRHLPTTAAVSVSSRILAGFWLGS
                     LGLGANCYEQRLPDSIWTAPAAYQRALLAGMWRGDGSWSFINGGPSVILEYGTVSREL
                     ADGVLRLLAGLGIVASVRVGRVAKSTCDTFWIRISGAQQVEQLLDFAPEQDRAVISAS
                     IARQARRIAPTGYNRDDERVWVRVVDTIRRPFTGAVYSLEVPGPHTFVTTGGLVTHNC
                     FPKDSLALKQLASNSGYHFQLLSAVIEVNELQKRRVVQKLQKHLGKLRGKKIALLGLA
                     FKADTDDMREAPSLVLTSRLLAEGAEVRAWDPVARPGELMKGAVLCDSVLEAVRGADA
                     AVIVTEWDELRGLASKDVHDAMARPLILDGRNLLDPAQTRAAGFAYEGIGRPSSPFEA
                     LPETPEPETTVSQ"
     gene            1393..2106
                     /locus_tag="VF225_07395"
     CDS             1393..2106
                     /locus_tag="VF225_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7145119.1"
                     /translation="MEAIILAGGQATRLGDAARGHPKALVPIAGHPLAEYQVSQFVRA
                     GVERVIVSCGAGQAALFEAKLAGLGAEIVAVAEPEPLGRGGGLRFAAAHREETGPIYA
                     FNGDELHDVDLRAFLEAHEDHGGAATIVVAPLTSQFGVVELEDNDRVTGFREAPRLPH
                     WVNAGVYVLGDEALARMPERGDHEQSTFPELAEEGKLFAFRHEGLWITVNTPKDLQRA
                     EEHYASHPELRPTLARGRS"
     gene            2224..2634
                     /locus_tag="VF225_07400"
     CDS             2224..2634
                     /locus_tag="VF225_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145120.1"
                     /translation="MSVDSPNLGGLDAWAFEPRKVDKPWGYELIWAETDRYVGKVLFV
                     KAGESLSLQFHRVKDESWLVHSGRARLELGSAGDPVLSQEVIGPGASFHFRPGSVHRV
                     TAIEDTTILEVSTPELDDVVRLEDRYGREGTSEP"
     gene            2658..2981
                     /locus_tag="VF225_07405"
     CDS             2658..2981
                     /locus_tag="VF225_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7145121.1"
                     /translation="MAGLTIGEAAAQTGWSPRMLRYLEAAGLVVPRRSGAGYRLYGLL
                     ELNQLRSLRELRRRHDVELTDVAFAARLEREPELRAAVGTWLAGTQLSALDWEQRKHE
                     RLLAA"
     gene            2995..4263
                     /gene="ahcY"
                     /locus_tag="VF225_07410"
     CDS             2995..4263
                     /gene="ahcY"
                     /locus_tag="VF225_07410"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7145122.1"
                     /translation="MATTKRHDVKDLALAPEGVRRIEWADRQMPVLAAIRERFDREQP
                     LSGYRISACLHVTTETANLARTLKAGGADVVLCASNPLSTQDDVAAALVDEYDIGVFA
                     IKGEDNDTYYAHIEAAVDHKPQLTMDDGADVIGVLHSARREQLGDIIAGTEETTTGVI
                     RLKALERDGALGFPVIAVNDAQTKHLFDNRYGTGQSTIDGIIRATNVLLAGKRFVVAG
                     YGWVGRGVASRARGMGSHVIITEVDPMRALEATMDGYEVLSMEKAAPIGDIFVTATGD
                     KSVIRKEHIQKMKDGAILANTGHFNVEIDIPGLRALATDTREARAFVEEFTMTGGERI
                     YLIADGRLVNLSAAEGHPALVMDMSFANQALSAEYAIQNAAELERKVYPVPEEIDKEI
                     ARLKLATMGVEIDTLTEEQEKYLASWDEGT"
     gene            4245..5207
                     /gene="mtnA"
                     /locus_tag="VF225_07415"
     CDS             4245..5207
                     /gene="mtnA"
                     /locus_tag="VF225_07415"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7145123.1"
                     /translation="MGRRNLDPEDIVRYEEDGPRVVLLDQRRLPDEEVELECGSVAEV
                     VVAIRELAIRGAPAIGVAAAYALALGGALGDDLDEATELLAASRPTAVNLAWALDQMR
                     DDPTPARARALHDEEVERCHTMAAHAAELFGPGTRALTHCNAGGLATGGYGSAVGALR
                     TAWERGSLERVWVDETRPLLQGARLTAWELEAAGIPHAVIADSAAASLMAAGDVDCVV
                     TGADRIAANGDTANKIGTYSLAVLAAYHGIPLYVVAPTSSIDLTTRTGAEIPIEERAA
                     SEITTRFAVRNPAFDVTPANLITAIVTEHGVHRAPYAQSLAALV"
     gene            5204..5950
                     /locus_tag="VF225_07420"
     CDS             5204..5950
                     /locus_tag="VF225_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7145124.1"
                     /translation="MKALILAAGYATRLRPLTDSIPKQLLPVGGRPMVDWILDRLGET
                     SADEVHLVTNARFAERFERWAEDKDVRIHNDGTTSNEDRLGAIGDIRFVDLDDDLLVV
                     AGDNLFDYSLADYEAYWRERDGSCIAVFDVGDPELAKKYGIVDVGENDRVVGFLEKPE
                     KPPTTLCATAMYLYEREHVRLVEQYLEEGNPPDQPGNYIAWLHTREPVYAYRFDGQWY
                     DIGDATQLLEADNRMRRLRGLPERAEYSLS"
     gene            5988..6650
                     /locus_tag="VF225_07425"
     CDS             5988..6650
                     /locus_tag="VF225_07425"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7145125.1"
                     /translation="MLDLLLPQRCLLCFLPGAQVCDGCTAALRRIDLPLCERCGAPTA
                     WPVRRCAECAGRRLAFTRARAALAYDDRLRPVVAAWKERGLRRLAGWAAAVVHDSLPR
                     PDAVCVVFVPGDPDRRLKRGHHAAERLASELAAFWELPLRPLLTRARGSPRQRGLSQL
                     ERRRNVVGAFFAHGRVPARIALVDDVYTTGATVNAAASALRKGGARGVEVVTFARTIR
                     IR"
     gene            6680..7228
                     /gene="raiA"
                     /locus_tag="VF225_07430"
     CDS             6680..7228
                     /gene="raiA"
                     /locus_tag="VF225_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7145126.1"
                     /translation="MRLQVKGKNHEVSDTIRRYAEEKMQKLDRQLHELAEVELELRVE
                     KNPSIADNQVAEVTVWTKGPTLRVKEASTDMKASIDQLTDKLLRQIDHARGRRRKDWH
                     PRDNGTPTGGPMSIPDEEEAGEQIVKVKQFSVKPMSPEEAVLQLELIGHDFFVFRSDE
                     SGDINVIYRRKNGGYGLIEPQG"
     gene            7343..7885
                     /locus_tag="VF225_07435"
     CDS             7343..7885
                     /locus_tag="VF225_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145127.1"
                     /translation="MGLFRRKPLHQRLAEEGGLVERSTVPLFTGVIPETGIHGIPRER
                     QYDAVAATEAPDVEGDAARFVALEDGSLLVEEGDGDLTPLADAIEQEIARPYRATAVR
                     RGETTWAIAAHRLRVVELPEPGGDEVELVLKGDEKTLVVDGNRSFGTMPELEQLADGD
                     AVVRAARLDDALWEVRVDPL"
BASE COUNT         1354 a         2647 c         2748 g         1290 t
ORIGIN      
        1 ggacctttct ctcgagagag cccagctcgg gagttgccgg aacggcacct acgttcctgc
       61 ctcgatcgcc gagggcgagg ctttctggcg gatcgtcggc ctctacatcg ccgaaggtca
      121 ttgcacggct gatgggaagc gccgccgact gcaatggtcg tttcatccaa gggacgagtt
      181 cgatctcgtc gaggaggtct actcgttctg ggccgctcag cgtgttcgcg cgagcgttcg
      241 ccatcttcca actaccgcag ccgtctcggt ctcctcgcga attctcgccg gcttctggct
      301 cggctcgctc gggctcggcg ccaattgcta cgagcaacgt cttcccgatt cgatctggac
      361 tgctccagcc gcgtatcagc gagccctgct ggccgggatg tggcgtggcg acggctcgtg
      421 gtctttcatc aacggcggtc ccagcgtcat cctcgaatac gggaccgtca gccgcgaact
      481 cgccgacggt gtgctcaggc tcctcgcggg tctcggcatc gtcgcgtcgg tgcgagtcgg
      541 ccgcgtggcc aagtccacct gtgacacgtt ctggatccgc atttccggcg cgcagcaggt
      601 cgagcaactt ctggacttcg cgcccgagca ggatcgtgcg gtcatcagcg cgtcgatcgc
      661 tcgccaggcc aggcggatcg cgccgaccgg ctacaaccgc gatgacgaga gggtttgggt
      721 ccgcgtcgtc gacacgatcc gccggccgtt cacaggtgcc gtctattccc tcgaggttcc
      781 cggtccgcat acgttcgtta cgacgggcgg tcttgtgacg cacaactgtt ttcctaagga
      841 ctcgctcgcg ctcaagcagc tcgcctcgaa ctcgggttac cacttccagc tgctgtcggc
      901 cgtgatcgag gtcaacgagc tgcagaaacg gcgcgtcgtg cagaagctgc agaagcacct
      961 cggcaagctc cgcggcaaga agatcgcgct gctcggcctc gcgttcaagg ctgacaccga
     1021 cgatatgcgc gaggcgccgt cgctcgtgtt gacgtcacgc ttgttggccg agggcgcgga
     1081 ggtgcgcgcc tgggatccgg tggcgcggcc gggcgagttg atgaagggcg ccgtgctctg
     1141 cgactcggtc ttggaggccg tccgaggcgc ggatgccgcg gtgatcgtca ccgagtggga
     1201 cgagctgcgc ggcctggcgt cgaaggacgt gcacgacgcg atggcgcggc cgctcatcct
     1261 cgacgggcgg aacctgctcg acccggccca aacgcgtgct gcgggttttg cctacgaggg
     1321 catcggtcgg ccgagctcgc cgttcgaggc gctgcccgag accccggagc ccgagacgac
     1381 ggtgtcgcaa tagtggaggc gatcatcctc gcgggcgggc aggcgacaag gctcggcgac
     1441 gcggcgcgtg gacacccgaa ggcgctcgtc ccgatcgcag ggcacccact cgccgagtac
     1501 caggtctcgc agttcgtgcg cgcgggcgtc gagcgcgtca tcgtcagctg cggcgccggc
     1561 caggctgcgc tgttcgaggc caagctcgcg ggactgggcg cggagatcgt tgccgtcgcc
     1621 gagccggagc cgctcgggcg tgggggaggc ttgcgcttcg cggcggcgca tcgagaggag
     1681 acggggccga tctacgcatt caacggcgac gagctgcacg acgtcgacct acgcgcgttt
     1741 ctcgaagcgc acgaggacca cggcggcgcg gcgacgatcg tcgtcgcccc cctcacgtcg
     1801 cagttcggcg tggtcgagct cgaggacaac gaccgcgtga cggggttccg cgaagcgccg
     1861 cgcctgccgc actgggtcaa tgccggcgtc tacgtcctcg gcgacgaagc gctcgcccgc
     1921 atgcccgagc gcggcgacca tgaacagtcg acgttcccgg agcttgcgga agagggaaag
     1981 ctcttcgcct ttcggcacga gggcctctgg atcaccgtca acacccccaa agacctgcag
     2041 cgggcggagg agcactacgc ctcccatccc gagctccgcc cgacactggc ccgggggcgc
     2101 agctgaccta aaaggccggg gtcagaccct tgccgttaag gacaccgctc cgctcgacag
     2161 gtcttgtgcc cggcacggaa gacgtcctgc gtcccattga tgaacggcat agagtcgcca
     2221 aagatgagcg tggactcgcc caatctcggc ggcctcgacg cctgggcctt cgagccgcgc
     2281 aaggtcgaca agccgtgggg ttatgagctg atctgggccg agaccgaccg ctatgtcggc
     2341 aaggtcctct tcgtaaaagc cggcgaatcc ctgagcctcc agttccaccg tgtgaaggac
     2401 gagtcgtggc tcgtccactc gggccgcgcc aggctcgagc tcggctcagc cggcgacccc
     2461 gtcctgagcc aagaggtgat cgggcccgga gcctcgttcc acttccgccc gggcagcgtc
     2521 catcgcgtca cggcgatcga ggacacgacc atcctggagg tctcaacgcc ggagctcgac
     2581 gacgtcgtcc ggctcgaaga ccggtatggg cgggagggaa cctccgagcc ttaacacgaa
     2641 cgtgttaagg tctgggcgtg gcaggactga cgatcggcga ggccgccgcc cagaccgggt
     2701 ggtcgccgcg gatgcttcgc tacctcgaag ccgcggggct cgtcgtccca cgccggagcg
     2761 gggccggcta tcgcctgtac gggctcctcg agctcaacca gctccgctcc ctgcgggagc
     2821 tccgacggcg ccacgacgtg gagctcacgg acgtcgcttt cgcagctagg ctcgagcgcg
     2881 agccggagct gcgggccgca gtggggacct ggctggcagg tacccaactg tccgccctcg
     2941 actgggaaca gcgcaagcac gagcggctct tggccgccta aacgagagga ctgaatggct
     3001 acgacgaaac ggcacgacgt gaaagacctg gcactcgcgc ccgaaggggt gcggcgcatc
     3061 gagtgggccg accgccagat gcccgtgctc gctgcgattc gcgagcgctt cgaccgcgag
     3121 cagccgctct ccggttatcg catctccgct tgtttgcacg taacgaccga gaccgcgaac
     3181 ctcgcccgca cgctgaaggc gggcggcgcc gacgtggttc tttgcgcgtc caacccgctc
     3241 tcgacgcagg acgatgtcgc agccgcactg gtcgacgagt acgacatcgg tgtcttcgcg
     3301 atcaagggtg aggacaacga cacgtattac gcacacatcg aggcggccgt cgaccacaag
     3361 ccgcagctga cgatggacga cggcgccgat gtcatcggcg tgctccactc cgcgcggcgg
     3421 gagcagctcg gcgacatcat cgcgggaacc gaggagacaa ccaccggggt catcaggctc
     3481 aaggcgctcg agcgtgacgg cgcgctcggt ttccccgtca tcgccgtcaa cgacgcccag
     3541 acgaagcacc tgttcgacaa tcgttacggc acgggtcaat cgacgatcga cggaatcatc
     3601 cgcgcgacca acgtgctgct ggcgggcaag cgtttcgtcg tggccggtta cggctgggtt
     3661 ggccgcggcg tcgcgtcgcg ggcccgcggg atgggctcgc acgtgatcat cacggaggtc
     3721 gatccgatgc gggcgctcga ggccaccatg gacggctacg aggtcctctc gatggagaag
     3781 gccgcgccga tcggcgacat cttcgtcacg gcgaccggcg acaagtccgt catccgcaag
     3841 gagcacatcc agaagatgaa ggacggcgcc atcctcgcga acacgggcca cttcaacgtc
     3901 gagatcgata tccccggctt gcgagcgctc gcgaccgaca cgcgcgaggc ccgggcattc
     3961 gtggaggagt tcacgatgac cggaggcgag cgcatctacc tgatcgccga cggccggctc
     4021 gtgaacctgt ccgctgctga gggccatccg gccctcgtta tggacatgtc cttcgcgaac
     4081 caggcgcttt ctgccgaata cgcgatccag aatgcagccg agctcgagcg gaaggtctat
     4141 cccgtgccgg aggagatcga caaggagatc gcacgcctca agctggcgac catgggcgtc
     4201 gagatcgaca cgcttacgga ggagcaggag aagtacctgg cttcgtggga cgaaggaacc
     4261 tagacccgga agacatcgtt cggtacgagg aggacggccc gagggtcgtc ctcctcgacc
     4321 agcgccggct gcctgacgaa gaggtggagc tggagtgcgg ttcggttgcg gaggtcgtcg
     4381 tggcgatccg cgagctggcc attcgcggcg cgcctgcgat cggtgtcgcc gcggcctacg
     4441 cgctcgcgct cggcggcgcg cttggggatg acctcgacga ggccaccgag ctgcttgctg
     4501 cttcgcgtcc gaccgcggtc aatctcgcgt gggcgctcga ccagatgcgg gacgacccga
     4561 ctcccgcgcg cgcccgcgct ctccacgacg aggaggtcga gcgctgccac acgatggccg
     4621 cccacgccgc cgaattgttc ggcccgggga cgcgcgcgct gacgcactgc aacgcgggcg
     4681 gactggcgac tggcggctat ggcagcgcgg tgggagcgct tcggaccgcc tgggagcggg
     4741 gatcgctcga acgcgtgtgg gtcgacgaga cccgtccgtt gctgcagggg gcacgcttga
     4801 ccgcgtggga gctcgaggcc gccggtattc cgcacgcggt gatcgccgat tccgcagccg
     4861 cctcgctgat ggccgcaggc gacgttgact gtgtcgtcac cggcgcggat cgaattgctg
     4921 ccaacggcga cacggcgaac aagatcggca cgtactccct cgccgtcctc gccgcgtacc
     4981 acgggatccc gctctacgtc gtcgcaccga cctccagcat cgacctgacc actcgcacgg
     5041 gggcagagat tccgatcgag gagcgtgcgg cgtccgagat cacgacacgc tttgcggtac
     5101 gcaatcctgc cttcgacgtc acaccggcaa acctcatcac ggcgatcgtc accgagcacg
     5161 gcgtccaccg cgcgccgtat gcgcagtcgc tcgcggcgct cgtttgaagg cgctcatcct
     5221 cgcggcgggc tacgcgacgc ggttgcggcc gctcaccgac tcgatcccga agcagctgct
     5281 cccggtcgga ggacggccca tggtggactg gatcctcgac cggctcgggg agacgagcgc
     5341 cgacgaggtg cacttggtca cgaacgcccg cttcgccgaa cggttcgagc gctgggcgga
     5401 ggacaaggac gtgcgcatcc acaacgacgg cacgacatca aacgaggacc ggctcggcgc
     5461 gatcggcgac attcgcttcg tcgatctcga cgacgacctg ctcgtggtgg ccggcgacaa
     5521 cctgttcgac tattccctcg ctgactacga ggcctactgg cgtgagcgcg atggaagctg
     5581 catcgcagtc ttcgacgtcg gcgaccccga gctggcgaag aagtacggga tcgtcgacgt
     5641 gggcgagaac gatcgggtcg tcggcttctt ggaaaagccc gagaagccgc cgacgacgct
     5701 ctgcgccacc gcgatgtacc tgtatgagcg cgagcacgtg cggctggtcg agcagtacct
     5761 cgaagaggga aatccaccgg accagccggg aaactacatc gcctggctgc acacgcgtga
     5821 gcccgtctat gcataccgct tcgacgggca gtggtacgac atcggcgacg caactcagtt
     5881 gctcgaggcg gacaaccgca tgcggcgact gcgcggcctg ccggaacgcg cggagtattc
     5941 actgagctga caccgacacg tcacacacgc cgacgtagcg tggtgacgtg ctcgatctgc
     6001 tccttccgca acgctgtctg ctctgttttc ttcccggcgc acaggtttgc gacggctgca
     6061 ccgctgctct ccgccgcatc gacctgccgc tctgcgagcg gtgcggtgca ccgaccgcct
     6121 ggcctgtgag gcgttgtgcc gagtgtgcgg gccgacggct cgccttcacg cgtgctcggg
     6181 cggcattggc gtacgacgac cgcttgcgtc cggtcgtcgc cgcctggaag gagcgtggtc
     6241 tacgccggct ggccggctgg gcggcggctg tcgtccacga ctcgctcccg cggcccgacg
     6301 cggtgtgtgt cgtgttcgtc ccgggcgatc cggaccgcag gctcaagcgc gggcatcacg
     6361 ctgctgagcg gctggcaagc gagctcgctg ctttctggga gctgccgctg cggccgctcc
     6421 tcacgagggc gagggggtct ccccggcagc gagggctgtc gcaactcgag cggcgacgga
     6481 acgtggtagg tgccttcttt gcgcacggcc gggtacccgc ccgcatcgcg ctcgtcgacg
     6541 acgtttatac gaccggtgcg actgtcaatg cggccgcgtc cgcactccgg aagggaggag
     6601 ctcgcggggt tgaggtcgtg acgttcgccc gcacgattcg cattcgctag ggtgagatct
     6661 ccggctcacg aggaggcgca tgcgacttca agtgaagggc aagaaccacg aggtcagcga
     6721 cacgatccgc cggtacgccg aggagaagat gcaaaagctc gaccgtcagc tgcatgagct
     6781 tgcagaggtc gagcttgagc tccgcgtcga gaagaaccct tcgatcgccg acaaccaggt
     6841 ggcggaagtg accgtctgga cgaaggggcc gactctccgc gtcaaggagg cgtcgaccga
     6901 catgaaggct tcgatcgacc agctgacgga caaactgctc cgccagatcg accatgcccg
     6961 cggcaggcgg cgcaaagact ggcacccacg tgacaacggc acacccaccg gaggtccgat
     7021 gagcattccc gacgaggaag aggcgggcga gcagatcgtc aaggtgaaac agttctcggt
     7081 caagccgatg agcccggagg aggctgtgct gcagctcgag ctcatcgggc acgacttctt
     7141 cgtctttcgc agcgatgagt ccggcgacat caacgtcatc taccgccgca agaacggcgg
     7201 ctacggcttg atcgagccgc agggttaatt cgtctgtgat cgcccgaacg acttcgatga
     7261 gcgtttcaga cggaggcgcg ggcggagccc gcacctcctc taggcggcac cgcgagcggt
     7321 gccttagcga gccgtcgggg tagtgggcct cttccggcgc aagccgttgc accaacggct
     7381 tgccgaggag ggcggtctcg tggagcggtc gactgtgccg ttgttcaccg gagtcattcc
     7441 ggagaccggc atccacggca ttccgcgcga gcggcagtac gacgcggttg ccgccaccga
     7501 ggcgcccgac gtggagggtg atgcggcgag attcgtcgcg ctcgaagacg gctcactgct
     7561 ggtcgaggaa ggcgacggcg acctgacccc gctcgcagac gcgatcgagc aggagatcgc
     7621 gcgcccgtac cgtgcaaccg cagtgcgtcg cggcgagaca acgtgggcga tcgctgcgca
     7681 caggcttcgc gtcgtcgagc tcccggagcc tggcggagac gaggtcgagc tggtgctgaa
     7741 aggcgacgag aagaccctgg tcgtggacgg gaaccgttcg ttcggaacga tgccggagct
     7801 cgagcagctg gccgacggcg atgcggtcgt ccgagccgcg cgcctcgacg acgctctgtg
     7861 ggaggttcgt gtcgatccgc tctaggtggg gcaagctacc ctaggctcta tgagctccca
     7921 gctcggttcc ttcgaaaagg tcctccgcat cggcgagggg cgccgcatga aacggctggc
     7981 cgagcaggcg gcctacatca cctcgatcga gcccgagttt cgcgagcttt ccgacgacc
//
LOCUS       DASVEU010000181         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_516056,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000181 DASVEU010000000
VERSION     DASVEU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516056"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..511)
                     /locus_tag="VF225_07440"
     CDS             complement(311..511)
                     /locus_tag="VF225_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145128.1"
                     /translation="MSREFEHGDEPRAEEIALHQRFLTLLGQMEAAQAEGNASEVRRL
                     GLPLERARKRWLRANVRVTRGE"
     gene            complement(555..1553)
                     /locus_tag="VF225_07445"
     CDS             complement(555..1553)
                     /locus_tag="VF225_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7145129.1"
                     /translation="MRIESSVTSISWIPSEAVEGLPKLPFTLGVAHYDSPPPDRVDNI
                     DTMHRADLFREANELKAWVEVEDGKILDYGHAGRGRIGLTRLKLGPKEIAVPAVAMPT
                     LQNSEVGDGWVRFTQTAGGRTGMPAPRSVRGKPYFQINSAIAWTTLALTIHQDGTSEH
                     ELAGASQFPRHWIYDKGGALVQKSGAIDFDKWYREAHEQNTPWGSEDSPAVVTAVETA
                     LERDLSLEIMGGDGKRRPQRLSAGDILVEQGDAKEGSNLVYLVLDGVLEVVVDGEVVG
                     ELGPGAIVGERSQLEDGARTATLRAKTAGKVVGVPAEEIDRERLEQVAAGHKREDG"
     gene            complement(1593..>3481)
                     /locus_tag="VF225_07450"
     CDS             complement(1593..>3481)
                     /locus_tag="VF225_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7145130.1"
                     /translation="LAQETSPYLLQHADNPVDWYPWGEEALTRAREEDRPILLSVGYA
                     ACHWCHVMAHESFEDGDTAALMNAHFVPVKVDREERPDVDSVYMDAVVSLTGHGGWPM
                     TVFLTPTGEPFFGGTYYPPEPRHGLPSFRQLLQAVADAWRDRRAEIERDAGAITDQLR
                     RVAEPSLEPLTSSVLADAARALRRQLDPVWGGFGNAPKFPPASVLEFLLRRGELEPAT
                     KTLDGMALGGMYDLVGGGFHRYSVDREWLVPHFEKMLYDNALLVPAYLHGWLVTGNER
                     YREVVEETLDYMLRELRLPAGGFASAQDADTEGVEGLTYTWTEADDVPAELLQPFEHG
                     RFIIRGELDRETRARLFEEREQRPKPLRDDKAIASWNGLALAALAESGRRLDRADYLE
                     AAGMLAGFLLGPLSTAEGRLFRSWRAGQAKHAGVLEDYADVANGLYELHVATGELRWL
                     VESRRLALLAVELFGDDEHGGFFLTASDGERLVARKKDFDDHPTPSGNSMLAYVLLRL
                     ARLWGEPELERQAVGVFRFVARLLPQAPSAFGHALNALDLHFSPPRELAVIGGPDTEL
                     AHAALAAFDPNAVVAFGPSEDVPLLQGKHLVDGRPAVYVCENFACQAPITQPSELVSA
                     SRGA"
BASE COUNT          550 a         1240 c         1110 g          581 t
ORIGIN      
        1 ggggtgcctc cgatcggcca cgaccggccc ttgcacacgg tcgtcgatgt ctccctgcga
       61 cggttcgaga cggtgtggtg tgccggcggc acgccccacg cggtcttccc cgtcgctacc
      121 gaagcgctga tcgccgcgat cccggacgtc gaagtccgcg agatctccta aaagtcggtg
      181 cagggttttg cccgtgctgc gggtctgctt gcttcgaggg ggcgcgcctc cgcggcggtc
      241 gacctgctag gaagtcacgg tcgcggcagc gcctcctcgt tctgaggcac gaaggcgcgt
      301 tccgaacgtc ttactcgccg cgcgtgacgc gcacgttcgc cctcagccag cgcttccgcg
      361 ctcgctcgag cgggagacca agacgccgca cttcggacgc gttgccttcc gcctgcgcgg
      421 cctccatctg gcctagcaac gtgagaaacc gctggtgcag cgcgatctcc tcggctctcg
      481 gctcgtcacc gtgttcgaac tcgcgactca tggtcagggc cgcaccgggc atcctcccca
      541 caagcgcgtg ccggtcagcc gtcctcgcgc ttgtgtcccg cggcgacctg ctcgagccgc
      601 tcacgatcga tctcctcggc ggggactccg acgaccttgc ccgcggtctt agcgcgcaaa
      661 gtcgcagtcc gagccccatc ctcgagttgc gagcgctcac ccacgatcgc acccgggccg
      721 agctctccca ccacctcgcc gtcgacgacc acctcgagca ccccgtcgag aaccaggtac
      781 acgagattgg agccctcctt cgcgtctccc tgctcgacga ggatgtcgcc cgccgacagg
      841 cgctgtggac gccgcttgcc gtcgccaccc atgatctcca gcgaaaggtc gcgctccagc
      901 gccgtttcga cagccgtgac gaccgcagga gagtcttcgc tgccccaggg cgtgttctgc
      961 tcgtgcgctt ctcggtacca cttgtcgaag tcgatcgctc cgctcttctg gacgagcgct
     1021 ccgcccttgt cgtagatcca gtggcgcggg aactggctgg cgcctgcgag ctcgtgctct
     1081 gaagttccgt cttggtggat cgtgagcgcg agagtcgtcc aggcgatcgc cgagttgatc
     1141 tggaagtagg gcttgccccg cacgcttctc ggcgccggca tgcctgttcg acctcccgcg
     1201 gtctgcgtaa agcgcaccca cccgtccccg acctcgctgt tctggagcgt cggcatggca
     1261 acggccggga cggcaatctc cttcggcccc agcttcagcc ttgtcaatcc gatccgcccg
     1321 cggcccgcgt gcccgtagtc caggatcttg ccgtcctcga cctcgaccca cgccttcaac
     1381 tcgtttgcct cgcggaagag gtcggcccgg tgcatcgtgt cgatgttgtc gacgcgatcg
     1441 ggcggcgggg agtcgtaatg ggccacgccg agcgtgaacg ggagcttcgg cagcccttcg
     1501 accgcctcgg acgggatcca ggagatagac gtgacggagg actcgatccg catagctgcc
     1561 cctttcgcga cgttactggt ttgcgatcga gcctatgccc ctctggacgc gctgacaagc
     1621 tcactgggct gcgtgatcgg cgcctgacag gcaaagtttt cgcagacgta gaccgccgga
     1681 cgcccgtcga cgaggtgctt cccctgcaag agtgggacgt cctcgctcgg gccgaacgcg
     1741 acaacggcgt tcggatcgaa cgcggcaagc gcggcgtggg cgagctccgt gtccggcccg
     1801 ccgatcaccg ccaactccct tgggggtgag aagtgaagat ccagagcgtt caatgcgtgc
     1861 ccgaacgccg agggcgcctg cggcaggagc cgcgcgacga accggaagac cccaacggcc
     1921 tgccgctcga gctcaggctc gccccacaac cgcgcgaggc gcaggaggac gtacgcgagc
     1981 atcgagtttc cggacggcgt gggatggtcg tcgaaatcct tcttgcgagc gacgagccgc
     2041 tcaccgtcgc tcgccgtgag gaagaagcca ccatgttcgt catcgccgaa gagctcgacc
     2101 gcgaggagcg cgagccgccg cgactccacg agccaccgca actctcccgt cgcgacgtgc
     2161 agctcgtaaa ggccgttggc cacgtccgcg tagtcctcga gcacgcctgc gtgcttcgcc
     2221 tggcccgcgc gccagctgcg aaagaggcgt ccctccgcgg tcgaaagcgg accaaggagg
     2281 aagccggcga gcattccggc cgcttcgaga tagtcggctc gatcgagacg gcggcccgat
     2341 tcggcgagtg ccgcgagcgc aagcccattc cacgacgcga tcgccttgtc gtcgcgaagc
     2401 ggcttcggcc gctgctcccg ctcctcgaag agccgcgcac gagtctcacg atcgagctcg
     2461 ccccgaatga tgaagcgccc gtgctcgaac ggctgcagca gctccgccgg aacgtcgtcc
     2521 gcctccgtcc acgtgtaggt caggccttcc acgccctcgg tgtctgcgtc ctgcgcggaa
     2581 gcgaaaccgc ctgccggcaa ccgcagctcc cgcagcatgt agtcgagcgt ctcttcgacg
     2641 acctctcggt agcgctcgtt ccccgtcacg agccagccgt gaagatacgc aggtacgagt
     2701 agcgcgttgt cgtacagcat cttctcgaaa tgcggcacga gccactcgcg gtcgacggaa
     2761 tagcgatgaa agccaccgcc gacgaggtcg tacatgccgc cgagcgccat gccgtccagc
     2821 gttttggtcg ccggctcgag ctcgccgcga cgcaacagga actcgagcac cgatgccggt
     2881 gggaacttcg gtgcattgcc aaagccgccc cagaccggat ccaactgccg ccgcagcgcg
     2941 cgcgccgcat ccgctagcac cgacgaggtg agcggctcca gtgacggctc cgccacgcgg
     3001 cgaagctggt cggtgatcgc ccccgcgtcg cgctcgattt ccgcgcgccg atcgcgccag
     3061 gcgtccgcta cggcctgcag cagttgcctg aagctcggca gaccgtgacg cggctcgggc
     3121 gggtagtacg tgccgccgaa gaacggctcg cccgtcggcg tgagaaagac ggtcatgggc
     3181 cagccgccgt gaccggtcag cgagacgacc gcatccatgt acaccgagtc gacgtcgggc
     3241 cgctcctcgc ggtcgacctt caccgggacg aagtgcgcgt tcatcagcgc tgcggtgtcg
     3301 ccgtcctcga aggactcgtg cgccatcacg tggcaccagt gacacgccgc gtagccgact
     3361 gagaggagga tcggcctgtc ttcttcacgc gcgcgcgtga gcgcctcttc cccccacgga
     3421 taccaatcga caggattgtc ggcgtgctgc agaaggtacg ggctcgtctc ttgggcgaga
     3481 c
//
LOCUS       DASVEU010000182         8834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1096305,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000182 DASVEU010000000
VERSION     DASVEU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096305"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VF225_07455"
     CDS             <1..1828
                     /locus_tag="VF225_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX7145131.1"
                     /translation="LLVSAALGTALILLAMVPPLQFRGWEWVAFALATPIVLWAGLPF
                     HRAAVRNARHLTATMDTLVSLGTLAAWTWSVVVLVAGLNADVYFEVAGVITTLILLGR
                     FLEARARRRSGAAIRALLELGAKEARLLRDGVEVLVPVDQLQVGDTFVVRPGEKVATD
                     GVVVEGDSAVDQSMLTGESMPVEVDPGAKLAGATINTYGRLVVRVTKVGAETALAQIA
                     RLVAEAQSGKAPIQRLVDRISGVFVPVVLVIALGTLAGWLAVGGNAGDAFTAAVAVLI
                     IACPCALGLATPTALLVGTGRGAQLGILIKGPEVLEQTRRVGTVVLDKTGTVTAGEMV
                     LVDVAPLNGATRKDALRFAGAVEAASEHPIARAIVAAAREEVGSLPPVTGFRNVPGVG
                     VHGVVEGHNVEVARRSGAITVSWEGEARARLVVQDTLKPTSREAVRQLEELGLKPVLL
                     TGDDESTARRIASEVGIERVIAEVLPQEKAAEVRRLQAAGEVVAMVGDGVNDAPALAQ
                     ADLGIAIGTGTDVAIEASDLTLVSGDLRGAADAIRLSRGTLRTIKGNLFLAFAYNVVA
                     IPLAVAGLLNPIVAAASMAFSSVFVVSNSLRLRRFHSTREAR"
     gene            1825..2109
                     /locus_tag="VF225_07460"
     CDS             1825..2109
                     /locus_tag="VF225_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX7145132.1"
                     /translation="MKHGYDKEALIKRLHRIEGQVRGIERMVEDDRYCIDILTQVAAV
                     NTALESLAFKVLDDHVSHCVTDALSSGDPKEAAAKSKELMEAVQRFARTR"
     gene            complement(2243..3997)
                     /locus_tag="VF225_07465"
     CDS             complement(2243..3997)
                     /locus_tag="VF225_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145133.1"
                     /translation="MMERASAKAVWQHTRGLLRPVRKLYLGAGAAVMLSTLITLAGPA
                     LVRYAVDAGIRKHNTHPLNVAGLIFLGLALSKPFVVRAQILFAARAGERFLDSLRVAA
                     FDKLQALPLGFFERERAGVLVSRLTSDVQSLNELVREALVEIFGSALQIVLTIVALVF
                     LSPKLALISLVALPILIASTWSFHHGAGRAYHAIRDRVAETFTVLQEGLAGVRVVQAF
                     RRERKTLARYRPRSQAQVSAWRRASFVNIRLFAFLPLAQAAALIVVLLAAASMYRRGE
                     ISIGTITAFVLYLIQLFDPIGRFSEWLGEFRQGLAALAKIVGLLQVPTSIAERPGAVE
                     LPREGVLALRDVTFGYQEGRPVVRDLTLELRPGEHVALVGATGAGKSTVAKLLTRQYD
                     PQEGRIELGGVDLRDATFESLHRRIVLLPQEGHLFSGTIADNVRLAHPEATDDEVRSA
                     LRRIGALDRFDSLPDGLETDVQTRGVRLSAGERQLVGIARVALADPAVIVLDEATSSL
                     DPATEAAVERALAAVAEGRTVVTIAHRLSTAERADRVAVMEHGRLVEVASHDELVEQG
                     DRYAGLWASWQAGLVAPA"
     gene            complement(3994..5739)
                     /locus_tag="VF225_07470"
     CDS             complement(3994..5739)
                     /locus_tag="VF225_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145134.1"
                     /translation="MGEHDVRRRAAWRFVRAVIRSQASGVVGAALSGLLWQAGAVSAP
                     LMVKYAIDHGVLTRDRHALLIWLGALLGVGLLEMTAGAVRHLYAIRNRARSDARVRDA
                     IFAHALRLDASYHDRVGPGELMSRASSDSEHVARMMDSIGHTIGYVLTVFAVAIVLLV
                     LDWRLALVVLIPLPLVSIAAWAYSRRYHARTERLQERWGAAATLVEETVSGIRVVKGL
                     GAGGPLASEFRTRSRSIVDRALDIARLDAVFMPTLEFLPLLGLLAVLWIGGRRVISGD
                     LSLGSFVAFNAYVAMLVWPLRVLGQRVTTLQKAVGASARISEVLDAEPQLRDPRHPER
                     LDRPVRGDVRLQGVQFGHEDGHPVLDGLDLHVAAGESVALVGATGSGKSTIAGLLARL
                     YDPDEGSVSLDGHDVRALRISDVRGAVALVFEETFLFTDTVRENIRFARPDARDEDVE
                     RAAELAGAAGFIADLPDGYDTSLGERGFSLSGGQRQRVAIARAILADPAVLVLDDATS
                     AVDATKEHEIRAALTTVMSGRTTLVIAHRPATIALADRVAVLDGGRIVEQGTHAELLR
                     SSVRYRALLALEDAA"
     gene            5758..6582
                     /gene="folP"
                     /locus_tag="VF225_07475"
     CDS             5758..6582
                     /gene="folP"
                     /locus_tag="VF225_07475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7145135.1"
                     /translation="MSNERPPIEERFARPSVMGVVNVTPDSFSDGGVHLQPAAAAAAG
                     WRMVDEGAAIVDVGGESTRPGSEGVSAEEELRRVVPVLEALHGAPISIDTAKADVARH
                     ALELDAELVNDVTALRADPQLAEVVAEAGAYLCLMHMQGEPRTMQRNPTYDDVASEVA
                     AFLEERLRFAVDSGIREERVALDPGIGFGKTVEQNFELIRRLDVLTALGRPVLVGFSR
                     KSSLGRVVGDPAATTGSLAASIAAAVAAYERGATILRAHDVREHVEALTVAAAVAG"
     gene            6579..6911
                     /locus_tag="VF225_07480"
     CDS             6579..6911
                     /locus_tag="VF225_07480"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7145136.1"
                     /translation="MIVEVHGLELFGRHGVNDDERRDGQTFLFDVTLEIAEPKEDAIE
                     ATIDYRLVRDIVRGVNDAESYRLLETLAAAVADALNTELRAKRVRVTVRKPGIAWAEW
                     TGVTCERP"
     gene            complement(6892..8379)
                     /locus_tag="VF225_07485"
     CDS             complement(6892..8379)
                     /locus_tag="VF225_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7145137.1"
                     /translation="MAPRLLIALALFFVAAAPARADLVELNWAHAHKGTLLARAAGGT
                     ELAHALRIWRVPAYQVAELRRAGVVRVSRTEQLIPTLATALQQATDPLVPLQWWRAAI
                     GSDQVDSPGAGKPVTIVDSGVDLTHPEFATRPNTTALNEQTTTDVDEDHGTEVSSVIA
                     APNNGVGVVGIYPDAVLRVWDASPFGFLSEGSAIQGIYEAARRGPGVINLSFGGEDDD
                     PLLDDAIRFAFHSGSLVVAAAGNDALSGNPPNFPAFYAHVLTVGATNERNQVAAFSSL
                     SPTVDLAAPGVHIPVAEPLARDPTGYSTEGNGTSFASPLVAGAAAWVWTVRPELDNTQ
                     LFELMRRSATDIGAPGFDNASGYGLLNLPSALTLKAPARDPQEPNEKARQIEPHGLFP
                     NGTPPLTAPGRTTGSISARVDRSEDPIDLYRVWAPAGRTLRAEVTGSVLVRLVARTTR
                     EHTLAVGKHGVATYRNKGKGVYVYAEVRPAVRLAEYKLRLKVARR"
     gene            8418..8783
                     /gene="folK"
                     /locus_tag="VF225_07490"
     CDS             8418..8783
                     /gene="folK"
                     /locus_tag="VF225_07490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7145138.1"
                     /translation="MVRAFVGLGSNLGERRETLRAAVGRLRGLAEVEVKGVSSFRDTD
                     PVDYLDQPRFLNGAVELETELSARALLGALLELERAFGRDRTTAPPHGPRTLDLDLLL
                     YGDATIDEPLLKVPHPRLQ"
BASE COUNT         1425 a         3103 c         2977 g         1329 t
ORIGIN      
        1 cctgctcgtg tcggcagcgc tgggtactgc actgattctg ttggcgatgg tgccaccgct
       61 gcagttccgc ggctgggagt gggtcgcttt cgcgctcgcg acgccgatcg tcctgtgggc
      121 cggcctcccg ttccatcgtg cggcagtgcg gaacgcgcgc cacctcacgg cgacgatgga
      181 cacactcgtc tccctcggaa cgctggcggc gtggacctgg tcggtggtcg ttctcgtcgc
      241 cggtctgaat gcagacgtct acttcgaggt tgcgggcgtg atcaccacgc tgattctgct
      301 tggccgtttt ctggaagcgc gggcccgcag gcgttcgggc gccgccatcc gcgcgctgct
      361 cgagctcggc gccaaggagg cgcgtctcct gcgagacggc gtggaggtac tcgtgccggt
      421 cgatcagctc caggtgggcg ataccttcgt cgtccgcccg ggcgagaagg tcgcgacgga
      481 cggcgtcgtc gtcgaagggg attccgccgt cgaccagtcg atgctcaccg gcgagtcgat
      541 gccggtcgag gtcgaccccg gcgccaagct ggccggagcg accatcaaca cgtatggccg
      601 gctcgttgtt cgtgtgacga aggtcggcgc ggagactgcc ctggcgcaga tcgcgcgtct
      661 cgtcgctgag gcgcagtcag gcaaggcgcc gatccagcgc ctggtcgacc ggatctcggg
      721 tgtgttcgtc cccgtcgtcc tggtcatcgc gctcggcaca ctggccggct ggcttgcggt
      781 cggcgggaac gccggcgacg cgttcaccgc ggcggtcgct gtgctgatca tcgcctgccc
      841 gtgcgcgctc gggctcgcga ccccgacggc actcctggtc gggaccggcc gcggggcgca
      901 gctcggcatc ctgatcaagg gccccgaagt cctcgagcag acgcggcgcg tcggcaccgt
      961 cgtcctggac aagacgggca cggtcacggc gggagagatg gtgctcgtgg acgtcgcacc
     1021 gctgaacggc gcaacgagga aggacgcgct tcgtttcgcc ggtgcggtcg aggccgcgag
     1081 cgagcacccg atcgcccgag cgatcgtggc agcggcgcgc gaggaggtgg ggagccttcc
     1141 acccgtgacc ggtttccgga acgttccggg cgtcggcgta cacggcgtgg tcgaggggca
     1201 caacgtcgag gtggcccgcc gcagcggggc gatcaccgtg tcctgggaag gcgaggcgcg
     1261 tgcacggctc gtcgtgcagg acacgctcaa gccgacgagt cgcgaggcgg tgcgtcagtt
     1321 ggaggagctc gggctcaagc cggtgctcct caccggcgac gacgagagca ccgcacgccg
     1381 catcgcgagc gaggtcggga tcgagcgcgt gatcgccgag gtgctgccgc aggagaaggc
     1441 ggccgaggtc cgccgtttgc aggccgcggg cgaggtcgtt gcgatggtcg gcgacggcgt
     1501 caacgacgcg ccggcgcttg cgcaggcaga ccttggcatc gcaatcggca ccggcaccga
     1561 cgtcgcgatc gaggcgtccg acctgacgct cgtttccgga gacttgcggg gcgcggcaga
     1621 tgcgatccgg ctttcgcgcg ggacgctgcg cacgatcaag gggaatctct tcctggcctt
     1681 tgcctacaac gtcgtcgcga tcccgcttgc ggtcgccggc cttctcaatc cgatcgtcgc
     1741 cgcggcttcg atggcgttct cgagcgtgtt cgtcgtctcg aactcgctcc ggctgcggcg
     1801 gttccacagc acccgggagg cacgatgaag cacggctacg acaaggaggc gctgatcaag
     1861 cgtctccatc gcatcgaagg acaggtccgc ggcatcgagc ggatggtcga agacgatcgt
     1921 tactgcatcg acatcctgac gcaggtcgca gcggtcaaca ccgcgctcga gagcctcgcc
     1981 ttcaaagttc tcgacgacca cgtgagccac tgcgtgacgg acgcgctctc ctcgggagat
     2041 ccgaaggagg cggccgccaa gagcaaggag ctgatggagg cggtgcagcg cttcgcacgc
     2101 acgcgctgaa caggatctga atcgcgagcc gtctcggcgg tcaccactga cccaaacagc
     2161 aagggtctga ccccagctcc ttgggacttc gtggttagtg tcacgcctcg aacggcaagg
     2221 gtctgacccc agctctttgg gactacgccg gtgccacgag gccagcttgc cacgacgccc
     2281 ataggccggc gtaacgatcg ccttgctcca ccaactcgtc atgggacgcc acctcgacta
     2341 ggcggccatg ctccatgacc gctactcggt ctgcccgctc cgccgtggac agccgatgcg
     2401 cgatcgtcac cacggtccgg ccttccgcta ccgcggccag cgctcgctcc actgccgctt
     2461 ccgttgccgg atccaggctc gacgttgctt cgtccaggac gatcaccgca ggatccgcaa
     2521 gcgcgacgcg cgcgatcccg acgagctggc gctcgcccgc ggacagacgc acgccccgcg
     2581 tctgcacgtc cgtctcgaga ccgtccggca acgagtcgaa ccggtcgagc gccccgattc
     2641 gccgcaacgc gcttcgcacc tcatcgtccg ttgcttccgg gtgggccagg cgcacgttgt
     2701 ccgcaatcgt cccgctgaac agatggccct cctgcggcaa aaggacgatg cgccggtgta
     2761 gtgactcgaa cgtcgcatcg cgcaggtcga cgccgcccag ttcgatccgc ccctcctgcg
     2821 ggtcgtactg ccgagtcagc agcttcgcga cggtggactt gcccgccccc gtcgcaccca
     2881 ccagcgcgac gtgctcgccg ggccgcagct ccagcgtcag atcgcgaacg accggtcgac
     2941 cctcctgata accgaaggtg acgtcgcgca acgcaagcac gccctcgcgc ggaagctcca
     3001 ccgcgcccgg ccgctcggcg atcgacgtcg gcacctgcag caagccgacg atcttcgcga
     3061 gtgccgccag tccttgcctg aactcaccca gccactcgct gaaccgtcca atcgggtcga
     3121 agagctggat cagatagagc acgaacgcgg tgatcgtccc gatcgagatc tcgccccgcc
     3181 ggtacatcga cgcggccgcc agcagcacga cgatcaacgc agccgcctgc gccagaggaa
     3241 ggaaagcgaa gagcctgatg ttcacgaagg acgcgcggcg ccatgcgctc acttgcgcct
     3301 ggctccgcgg ccggtagcgg gcgagcgtct ttctctcgcg ccgaaacgct tgcacgaccc
     3361 gaacgccggc caacccttcc tgcagcacgg tgaacgtctc cgccacgcga tcgcgaatcg
     3421 cgtgataggc acggccggca ccgtgatgga agctccaggt cgaggcgatc aggatcggca
     3481 gcgccacgag ggagatcagc gccagcttcg gcgacaggaa cacgagcgcc acgatcgtca
     3541 ggacgatctg cagggcgctt ccgaaaatct cgacgagcgc ctcacgcacg agctcgttca
     3601 gcgactggac gtcggaggtg agccgcgata cgagcacgcc ggcacgctca cgctcgaaga
     3661 agccgagggg cagcgcctgc aacttgtcga aggccgcgac gcgcaggctg tcgaggaacc
     3721 gctcgcccgc ccgcgcggcg aagaggatct gcgcgcgtac cacgaagggt ttcgacagcg
     3781 caagcccgag gaagatcagc ccagcaacgt tcagaggatg cgtgttgtgc ttccggatcc
     3841 ctgcgtccac cgcgtaccgc accagagccg ggcctgcgag cgtgatcaac gtcgagagca
     3901 tcactgccgc acccgcgccg aggtagagct tccgtaccgg tcgcagaagc ccccgtgtgt
     3961 gctgccagac agccttcgcc gaggccctct ccatcatgcc gcgtcctcca acgcgagcaa
     4021 ggcccgatac cgcaccgacg accgcagcag ctccgcgtgc gttccctgct ccacgatccg
     4081 tccgccatcc agcaccgcga cccggtcggc cagcgcaatc gtcgccggac gatgcgcgat
     4141 caccagtgtc gtccgcccgg acatgaccgt tgtcagtgcc gcgcggatct cgtgctcttt
     4201 cgtcgcgtcg accgccgacg tcgcgtcgtc caggacgagc acggcgggat cggcgaggat
     4261 cgcccgcgcg atcgccaccc gttgacgctg gccgcccgac aacgagaacc cacgctcacc
     4321 gagcgaagtg tcgtacccgt cggggagatc ggcgatgaac cctgccgccc cggccagctc
     4381 cgcggcgcgc tcgacgtcct cgtctcgagc atccggccgt gcgaagcgga tgttctcgcg
     4441 caccgtgtcc gtgaagagaa acgtctcctc gaagacgagc gcaaccgccc cgcggacgtc
     4501 cgagatccgc agcgcgcgga cgtcgtgacc gtcgagcgag acgctgccct catcggggtc
     4561 gtaaaggcgc gcaagcagtc ccgcgatggt gctcttgccc gacccggtcg ctccgaccag
     4621 cgcgaccgac tcgccggcag caacatgaag gtcgagcccg tccaggaccg gatgaccgtc
     4681 ctcgtggccg aactgcaccc cctggagccg cacgtcacca cgcacgggac ggtccagtct
     4741 ttccggatgt cgcggatcgc gcagttgcgg ctcggcgtcc agcacctcgc tgatccgcgc
     4801 gctcgcaccg actgccttct gcaacgtcgt cacacgctgg ccgagcacgc gcaagggcca
     4861 taccagcatt gcgacatagg cattgaacgc gacgaacgag ccgaggctca gatcgccgga
     4921 gatcacacgt cgtccgccga tccagagcac ggcgaggagc ccgagcagcg gcaggaactc
     4981 cagcgtcggc atgaagaccg cgtcgagccg cgcgatgtcg agcgcacggt cgacgatcga
     5041 gcggctgcgc gtgcggaact cggacgcgag cgggccgccg gcgccgagcc ccttcacgac
     5101 ccggatgccg ctcaccgtct cctcgacgag cgtcgcagcc gcgccccagc gctcctgcag
     5161 gcgttcggtg cgcgcgtggt agcgccgcga gtacgcccac gcggcgatgg agaccagcgg
     5221 cagcgggatc agcacgacca gagcgagccg ccagtcgagg acgagcagga cgattgccac
     5281 cgcgaatacc gtcaggacgt agccgatcgt gtggccgatc gagtccatca ttcgcgcgac
     5341 gtgctcggag tccgacgacg cgcgcgacat cagctcgccc ggcccgactc gatcgtggta
     5401 gctcgcgtcc agccgcagcg catgcgcgaa gatcgcgtca cgaacgcgcg catccgaccg
     5461 tgcccggttg cgaatggcgt acaggtgccg caccgcgccg gcggtcatct cgagcagccc
     5521 gactccgagc agcgcgccga gccagatcaa cagggcgtgc cggtccctcg tcaacacgcc
     5581 gtggtcgatc gcgtacttca ccatcagcgg cgccgagact gctccggcct gccagagcag
     5641 gccggacaga gcggcgccga cgaccccgct tgcctgagaa cgaatcacgg cgcgcacgaa
     5701 cctccacgcc gcgcgccgac gcacgtcgtg ttcccccatc ggtcaaagta tccagtggtg
     5761 tcaaacgagc gtcctccgat cgaggagcga tttgcacgtc cgtccgtgat gggagtcgtg
     5821 aacgtgaccc ccgattcctt ctccgacggt ggtgtgcacc tccagcccgc tgccgccgct
     5881 gccgccgggt ggcgcatggt cgacgagggc gcagcgatcg tggacgtggg gggcgagtcc
     5941 acgcgtcccg gttcagaagg agtctccgcg gaggaggagc tacgccgcgt cgtgccggtg
     6001 ctcgaagctc tgcatggcgc gccgatctcc atcgacaccg cgaaggccga tgtcgcccgc
     6061 cacgcgctcg agctcgacgc cgagcttgtc aacgacgtga ccgcgctcag agccgacccg
     6121 cagctggcgg aggtcgtcgc cgaggccggc gcctaccttt gcctgatgca catgcagggc
     6181 gagcctcgca cgatgcagcg gaacccgacc tacgacgacg tcgcgtccga ggtcgccgcc
     6241 ttcctcgagg agcgactccg cttcgcagtc gactccggga tccgggaaga gcgggtcgcc
     6301 ctcgatccgg gcatcgggtt cggcaagacc gtcgaacaga acttcgagct catccggcgc
     6361 ctcgacgtcc tcaccgcgct cggccgcccg gtcctcgtcg gcttctcgcg gaagagctcg
     6421 ctcggccgag ttgtcggcga ccccgccgcc accaccggct ccctcgccgc cagcatcgcc
     6481 gcggcggtcg ccgcctacga gcgcggcgcg acgatcctcc gcgcccacga cgtgcgcgag
     6541 cacgtcgaag ccctgaccgt cgcagcggcg gtggccggat gatcgtcgag gtgcacggcc
     6601 tcgagctctt cgggcgtcac ggcgtcaacg acgacgagcg ccgcgacggt caaactttcc
     6661 tcttcgacgt gacgctcgag atcgccgagc ccaaggagga tgcgatcgag gcgacgatcg
     6721 actaccgcct cgtgcgggac atcgtccgtg gcgtcaacga cgccgagagc taccggttgc
     6781 tcgagacgct cgccgcggcg gttgccgacg cgctgaacac cgagctgcgg gccaagagag
     6841 ttcgcgtcac cgtgcgtaag ccggggatcg cgtgggcgga atggacgggc gttacctgcg
     6901 agcgaccttg aggcgcagct tgtattcggc gaggcgcaca gccggacgaa cctcggcata
     6961 gacgtacacg cctttgccct tgttccggta cgtcgcgacg ccgtgcttcc cgacagcgag
     7021 cgtgtgctcg cgcgtggtgc gcgccaccag gcgcacgagc acggagcccg tcacctccgc
     7081 ccgcagcgtg cgcccggccg gcgcccagac gcgataaaga tcgatcggat cttcgctgcg
     7141 gtcgacgcgc gccgagatcg acccggtcgt gcgcccgggc gccgtcagcg gcggtgttcc
     7201 gttcgggaac aagccgtgcg gctcgatctg gcgcgccttc tcgttcggct cctggggatc
     7261 gcgcgcaggc gccttgaggg tgagcgcgct cgggaggttg agcagcccgt agccggatgc
     7321 gttgtcgaat cccggcgcgc cgatgtccgt agccgaccgg cgcatcaact cgaacaactg
     7381 cgtgttgtcg agctccggtc gcactgtcca tacccaggcg gccgcgccgg caaccagcgg
     7441 tgacgcgaag ctcgtgccgt ttccctcggt gctgtatccc gtggggtccc tcgccaacgg
     7501 ctccgccaca ggaatgtgca caccgggcgc cgcaagatcg acggtcgggg acaacgacga
     7561 gaacgcggcg acttggttgc gctcgttcgt ggcgccgaca gtcagaacgt gcgcatagaa
     7621 cgccgggaag ttcggtgggt tgccgctgag cgcgtcgttg ccggctgcgg ccacgacgag
     7681 cgagcccgag tggaacgcga accggatcgc atcgtccagc agcgggtcgt cgtcctcacc
     7741 gccgaagctg agattgatca cgccggggcc gcgccgcgcc gcctcgtaga tcccctggat
     7801 cgcagagccc tcactgagaa agccgaacgg gctcgcatcc cacacgcgga ggaccgcatc
     7861 gggatagatc ccgacgacgc cgacgccgtt gttcggcgcg gcgatgacgg agctgacctc
     7921 ggtcccgtgg tcctcgtcga cgtcggtcgt cgtctgttca ttgagcgccg tcgtgttcgg
     7981 ccgcgtggcg aactcgggat gggtcagatc gacgccggag tccacgatgg ttacgggctt
     8041 gcctgctcct ggcgagtcga cctggtcgga cccgatcgcc gcgcgccacc actgcagtgg
     8101 gacgagcggg tcggtcgctt gctgcagggc cgtggcgagg gtcggaatga gctgctccgt
     8161 ccggctgacg cggacgaccc cggcccggcg aagctctgct acctggtacg ccggcacccg
     8221 ccagatccgc agcgcgtgcg cgagctccgt tccaccggcg gcgcgggcca gcagggtccc
     8281 cttgtgtgcg tgcgcccagt tgagctcaac gaggtcagcc cgggcaggag cagccgccac
     8341 gaagaacagg gccagggcga tcaggagcct gggcgccacg tgttcgcaga gtagtcgcga
     8401 ttacctatgg tccgggcatg gtccgtgcct tcgtcggcct gggatccaat ctcggtgaga
     8461 gaagggagac gctgcgggca gccgtcggcc ggctgcgtgg gctcgccgag gtcgaggtga
     8521 aaggcgtctc gagcttccgg gacaccgatc ccgtcgacta tctcgaccag cctcgttttc
     8581 tgaacggagc ggtggagctc gagacggaac tttccgcgcg ggcgctcctg ggggccctgc
     8641 tcgagttgga gcgggccttc gggcgagacc ggacgaccgc gccccctcac ggcccgcgca
     8701 cgctcgacct cgacctcctc ctctacgggg acgcgaccat cgacgagccg ctcctcaagg
     8761 tcccgcaccc gcgcctacag taacggcggt tcgtgctcga gccgctcgca gagctggatc
     8821 ccgacctgga cgta
//
LOCUS       DASVEU010000183        12890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1290400,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000183 DASVEU010000000
VERSION     DASVEU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290400"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VF225_07495"
     CDS             complement(<1..1066)
                     /locus_tag="VF225_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145139.1"
                     /translation="MDGRPTAERALVPLRRNRDFVLLQIGQALSTVGSEASGIAYTLL
                     VLALNGSPAKAGLAAFARLVPYALFALPAGVASDRFNRKWLMIWADLVRVLALASLGL
                     SIATGHVTFPQVLIVAFVEGTMFVVFNIAEIGAVRSVVPALQLQRAFATEQARMSSVY
                     LAGPSLGGALFGLARSLPFLVDAVSYAFSTGTLLVMRTPFQEDREESETAGIWTQIRE
                     GLAWLWGHAFLRTCALLFVGGNFLFGALELVLIVAAKRQGLSSAAIGALVAVTGAFSL
                     LGSIAAPRFLRLLPMRAVLVLSAWLALGIVAFVIEPNVFVLVAGTAPLIFFNPTVNAM
                     VIGYRVAIVPDRLQGRVNSVA"
     gene            1116..4328
                     /gene="mfd"
                     /locus_tag="VF225_07500"
     CDS             1116..4328
                     /gene="mfd"
                     /locus_tag="VF225_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7145140.1"
                     /translation="MDHTTHTILHPFVAELRATERLKGFAEAFPAPARVSEPALPLLL
                     ASLYEELDRPLVLLAPEDQDARDAAEGAAWFLGEDRVAMLPSRGVNWGSGLDPAPHLV
                     GERARALDVLAAGGLVAASAAALAEPMPPPQARPQPIRIAPGEETSLEQLSETLALAG
                     YERVERADERGQFAVRGGLVDVFPTTGREPLRIEFWGDEIEQMRAFSPFTQRALHPVE
                     TAVIYPAAERRLDLFEPTLVGEDQPIPIPTDLVRAVPGGPDLVWHADEVRTVWEEEGL
                     EPVPFGKAALLDTLPAGQPFAFEAQRPALVARGLSEAENELAAFVRSGQRVVVAFPHL
                     GEAMRQQNLLRRIDARVIAEGEELPREAGLIFAVTPARRGFVWRDLGLVLLPDTQVFR
                     KRPPRERRVGGRALQSFADLRSGDYIVHEDHGVGKLLGFETKEVAGVTRDYLFLGFRG
                     DDRLYVPHEQLGKVSRYIGADNSAPALSKLGGKAWDNLKTRARESVRELAGELLALYA
                     QRQQAPGVAYDLRHEYLERLEAEFPYRETPDQQAAIEAVKEDLEAPRPMDRLVCGDVG
                     FGKTEVAIRAAFAVAVNGKQTLMLAPTTILAEQHWHTFRDRYRDFPVRVEMVSRFRSP
                     REVKQVLADFQAGKVDVLIGTHRVLSRDVIPKELGLVILDEEQRFGVAQKELLRSLRL
                     EVDVLTLSATPIPRTLHMSLSGLRDISIIETPPEGRRPIRTTVSEYDEEAIKLALERE
                     VERGGQAFYLHNRVETIDEVLEKLQQLAPNLRFLAAHGQMRERELEEKMHGFLRGDAD
                     VLVSTTIIESGLDIPQANTLIVERADQLGLSQLYQIRGRVGRSDVTAHAYLFYPDMSE
                     LTPEARARLATLADNTELGAGFAIAMRDLEIRGAGNLLGDEQSGHVAALGFELYVEML
                     AEAVAELSGERRVAARPVRVDARVDAYVPAEYIASEALKIDLHRRLALSESEDELREL
                     QAGTEDRFGPLPEPVENLFLIQEAKLKLARAGADYFVFRGGKASVGQMVLGSDELRDL
                     RRRVDTAVYTIANREVSLREEGFPQALRLVDAILAARQAA"
     gene            4357..5376
                     /locus_tag="VF225_07505"
     CDS             4357..5376
                     /locus_tag="VF225_07505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7145141.1"
                     /translation="MKRSFLLALISLVLVVAGCGGTSTATLSSNDAAVVGSEQVTKDQ
                     FQSLMDRAKTSYVAQKRPFPKPGSTEYEQLKGQAVTFLIQRAEFAQEAGAMGIKITDD
                     KVNKRIEQLKKQFYGGSEQRYEKTLKQQGLTADQAKEEVRAQLISEELFKKVTGDVKV
                     SKDEVKAYYNSHKSQYGQPQTRDVRHILVTKKALADSIYAQLKAGANFAKLAKKYSKD
                     PGSASNGGKLTISKGQTVPAFDKTAFSLKKGELSAPIHTQYGYHIIQALSDVKAAQTT
                     TLSKVESSIKQQLEQQKKNQVMTKWVDKKKKSYCKSGIKYQVGYQPNPDPCATLTGTT
                     TTTSQ"
     gene            5377..6171
                     /gene="mazG"
                     /locus_tag="VF225_07510"
     CDS             5377..6171
                     /gene="mazG"
                     /locus_tag="VF225_07510"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX7145142.1"
                     /translation="MGLAEALVDLQELTERLRRECPWDREQTERTIVPHTVEEAYEVA
                     DAAIGGDDAKLADELGDLLFQVYFLALLLAEKGQPGLEEIARGVHEKLVRRHPHVFGG
                     PELDSPGEVRRTWESSKRDLEGRAGIFHDVPESLPALLYARKVQRRAAAVDFEYPDTA
                     GALADLDDEVRELREALTEAGETPPETEPPAHVFEEMGDVLFAAVNVARRLNVDPELA
                     LRAMSRRFVERVERAERTAAAEGKSFAELALEEQDRYFDRAKEEFK"
     gene            6168..7454
                     /gene="eno"
                     /locus_tag="VF225_07515"
     CDS             6168..7454
                     /gene="eno"
                     /locus_tag="VF225_07515"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7145143.1"
                     /translation="MSASSIARVHGRQILDSRGNPTVEVDVVLESGASGRAAVPSGAS
                     TGRFEAVELRDGDKRSYRGKSVLNAVRNVETEIAGAVTGLDAGDQRALDLTLIELDGT
                     KNKSRLGANAILGVSLATAKAGAAHAGVSLFRWIGGAGAHVLPVPMMNVINGGAHAQN
                     SLDLQEFMVVPAGADSFAEGLRIGAETFHALKDVLHERGLSTGVGDEGGFAPELDSTE
                     AAIEAVLEAAERVGHRDRVAIALDPASTEFYRDGAYHLERQGQVMSAEELIDLYASLS
                     DRYPVVSLEDGLAEDEWGAWKTMTERLGDRLQLVGDDLFVTNVERFQRGIDEGVANAI
                     LVKVNQIGTLTESLDAIELARANGYAVVISHRSGETEDTTIADLVVATGAGQIKTGAP
                     SRTDRVAKYNQLLRIEEELGDAAVYPGWRAFPRQQR"
     gene            7480..8901
                     /gene="pyk"
                     /locus_tag="VF225_07520"
     CDS             7480..8901
                     /gene="pyk"
                     /locus_tag="VF225_07520"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7145144.1"
                     /translation="MASMTPRRTKIVATIGPACSTPERLRELVGAGMDAARLNLSHGT
                     HEDHAARAKLVRDVQEEVGRPLALIGDLQGPKFRVGGLDQPLILVQGSEVTVVTTGDG
                     AGNGELPVAPAAIGEVLRPGHEVLIDDGMIRLRVDAVESGRFRCGVVVGGTVTSHKGV
                     NLPGVPIAIPSLTRKDLEDLEFALELGCDFIALSFVRSAADVRDLKALIEQEGSTAHV
                     IAKIEKSEAVDALDDVLEEADAVMVARGDLGVEIGPELVPLVQKRIISKALERGKPVI
                     TATQMLESMIHHPEPTRAEASDVANAVLDGTSAIMLSGETAMGEYPVQAVQTMDRIAR
                     AVEPSLGYRHQIPEATEDPTIGNAMSNAACDLAEALGAKAMLVPTFTGRTASAVARLR
                     PRRPIVALTHKQWSVHQMAIEWGVTPIWIPECKDVEDLWNRSLDAAKEAGLIESGDTV
                     VITAGTAVNIPGSTNVIKVDVVL"
     gene            8963..9286
                     /locus_tag="VF225_07525"
     CDS             8963..9286
                     /locus_tag="VF225_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145145.1"
                     /translation="MAAQASRRPPLATLARRWFAVLLVVAIGYAYYHPLRSWLETRHE
                     LSLRRAEVGRLSSERRGLQDRLQFSASSDALAREARRLGFIRPGEHLFIVKGIQAWKK
                     AHPDR"
     gene            9322..9798
                     /locus_tag="VF225_07530"
     CDS             9322..9798
                     /locus_tag="VF225_07530"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX7145146.1"
                     /translation="MASLAKDDEDRAAVEHQLGRPPRAFRRVVVRCPFGHPAVTEQHA
                     FDDNGEPFPTTFWLTCPQLVAAVSRLEATGGVERWTRAAAADPRLAASLERAHAEQRR
                     LRPELDLGVGGARAGAGTLKCLHAHVAFALARPGYELGEQILAEVDPAWPSVCCAP"
     gene            9817..10131
                     /locus_tag="VF225_07535"
     CDS             9817..10131
                     /locus_tag="VF225_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145147.1"
                     /translation="MSVVVDVARREWEDGYRRFEAASRDPIAADRLNAQLEVVTGELR
                     RRIGQTFTLEELVAIYDRAEAWVRDVVSEHAATPGWPRTLALVEDAAFHLYQRGAVDY
                     KP"
     gene            10128..10355
                     /locus_tag="VF225_07540"
     CDS             10128..10355
                     /locus_tag="VF225_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145148.1"
                     /translation="MNALTPVERNERRRLRRRQETIRWSIRVLLIVLVFSLGVALGQA
                     IQDNPRPGRTVTSDRTLHVPSGGPQGSTITP"
     gene            10477..11061
                     /locus_tag="VF225_07545"
     CDS             10477..11061
                     /locus_tag="VF225_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145149.1"
                     /translation="MATREPGPRRVAAGESDWLTLGQAAKFLGVAQSTIRKWSDVGRV
                     PAFYTPGGHRRYKRGDLDAFLERSGPGKPARGPLVLLVDDDAQVREVVRINLEMEGYA
                     VREAANAEDGLAALEDEAPDLILLDVMMPQVDGWEMLRRVQERHGVGSIPVVMFSGQL
                     DAGNEAVQRGAQGFVGKPFDLRALIEQTKQIVPA"
     gene            11184..11636
                     /locus_tag="VF225_07550"
     CDS             11184..11636
                     /locus_tag="VF225_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7145150.1"
                     /translation="MLSAVYRRWGVFVLTVIAIALADWSSMGLKSLVDRPRPPLRYPE
                     PKTLVPLPHDASFPSGHAATSFAAATMLSLSFPRFAPFLYVLAAAIAFSRVYVGVHYP
                     LDVIGGAALGVLIAIALRVLMTRRARGRSSALQAEPPRPEAKTRRSRA"
     gene            complement(11802..12185)
                     /locus_tag="VF225_07555"
     CDS             complement(11802..12185)
                     /locus_tag="VF225_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145151.1"
                     /translation="MRLTWVSGLILAVSAFTDWYVGSGPGPTTSVIGWHTGALGKLVF
                     VIGLAVLALVILRETGIELPATVPESLVIIGLGALSTIFVLIRLISVPDEFFGWRGRG
                     IGIFISLIASLLVIAAGLLRAGEEV"
     gene            12357..12569
                     /locus_tag="VF225_07560"
     CDS             12357..12569
                     /locus_tag="VF225_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145152.1"
                     /translation="MMEGLHTSRAAPDEYVEFRAAGDPAKGEFHCSECGYGVTIVRAL
                     PICPMCGGTSWEQTSWSPFGRASSLQ"
     gene            complement(12583..12822)
                     /locus_tag="VF225_07565"
     CDS             complement(12583..12822)
                     /locus_tag="VF225_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145153.1"
                     /translation="MAQPARRHVAAPPEDPPVDPEAIDRAYHYHRARRNARLRRKREK
                     SNARLRFFITFTLLVGLSLFLLLTIWHEVQKVFGL"
BASE COUNT         2239 a         4272 c         4397 g         1982 t
ORIGIN      
        1 tggcgacgct gttcactcgt ccctgcaacc ggtcgggcac gatcgccacg cgatagccga
       61 tcaccatggc gttgacggtc gggttgaaga aaatcagtgg agctgttccc gcgacgagaa
      121 cgaacacgtt gggctcgatc acgaacgcga cgatcccgag cgccagccac gccgacagca
      181 caagaacggc gcgcattggg agcaggcgca aaaagcgcgg cgcggcgatg gagccgagca
      241 gcgagaacgc accggttacg gccacgagag caccgatcgc cgcggaggag aggccctggc
      301 gcttggcggc gacgatcagc acgagctcca acgcgccgaa gaggaagttc cctccgacga
      361 agagcagcgc gcacgtgcgg agaaatgcgt gtccccacag ccaggcgagc ccttccctga
      421 tctgcgtcca gatgccggcc gtctcgctct cctccctgtc ctcctggaac ggcgtcctca
      481 taacgagcag cgttcctgtc gagaacgcgt aggagaccgc atccaccaga aacggcagcg
      541 atcgcgcgag cccgaacagt gcgcccccga gcgatggccc cgcgaggtag acgctcgaca
      601 tccgcgcctg ctcggtcgca aaggcacgct ggagctgcag cgcaggcacg accgaacgga
      661 cggcgccgat ctccgcgata ttgaagacga cgaacatcgt cccttcgacg aaggcaacga
      721 tcaggacctg gggaaacgtg acgtgccccg ttgcaatgct gagccccagc gaggcgagcg
      781 cgagcacccg cacgaggtcg gcccagatca tcaaccactt gcggttgaag cggtcggacg
      841 caaccccggc agggagggcg aagagcgcat agggaacgag gcgcgcgaag gccgcaaggc
      901 ctgccttcgc cggtgacccg ttgagcgcga ggaccagcag cgtgtaggcg atccccgacg
      961 cctccgagcc gacggtcgac agcgcctggc cgatctggag gaggacgaag tcccgattcc
     1021 gtcggagcgg aaccagcgcg cgctccgcgg tgggtcgacc atccatgcgg gggtaccatg
     1081 acaagcgccg aactcgcggg gttcggcctt tcgtcatgga ccacacgacc cacacgatcc
     1141 tgcacccgtt cgtagcggag ctccgcgcga ccgaacgcct caaaggcttc gctgaggcgt
     1201 tccccgcgcc tgcccgcgtc tccgaaccgg cgctgccact actgctcgca tcgctctacg
     1261 aagagctcga ccgcccgctc gtcctcctcg ctccggagga tcaggacgcc cgcgacgcgg
     1321 ccgagggcgc tgcctggttt ctgggagagg accgcgttgc gatgctgccg agccgcggcg
     1381 tcaactgggg ctccggcctc gatcccgcgc cacacctcgt cggtgagcgc gcacgagcgc
     1441 tcgacgtgct cgcggcgggc gggctcgtcg cagcttcggc ggctgcgctc gccgagccga
     1501 tgccgccgcc gcaagcgcgg cctcagccga tccggatcgc gccgggcgag gagaccagcc
     1561 tcgagcagct ctcagagaca ctcgcgctcg ccggctacga gcgagtcgag cgcgccgacg
     1621 agcgtgggca gttcgccgtc cgcggcggtc tcgtcgacgt cttcccgacg accggccggg
     1681 agccccttcg catcgagttc tggggcgacg agatcgaaca gatgcgtgcg ttctcgccgt
     1741 tcacgcaacg cgcgctgcac ccggtcgaga cggcagtcat ctatccggcc gcggagcgcc
     1801 gactcgatct cttcgagccg accctcgtcg gcgaggacca gccgatcccg atcccgaccg
     1861 atctcgtgcg tgccgtgccc ggcggccccg acctcgtgtg gcatgccgac gaggttcgca
     1921 cggtctggga ggaagagggc ctcgagccgg tgccgttcgg aaaggcagcg ctgctggaca
     1981 cgctgccggc aggtcagccg ttcgcgttcg aggcgcagcg gccggctctc gtggcgcgcg
     2041 gcctctcgga agcggagaac gagctggccg cattcgttcg ctcgggacag cgcgtcgtcg
     2101 ttgccttccc acacctcggc gaggccatgc ggcagcaaaa ccttcttcgc cgcatcgacg
     2161 cgcgcgtcat cgcagaagga gaagagctac cgcgcgaagc ggggctcatc ttcgcggtca
     2221 cgcccgcgcg ccgcgggttc gtgtggcgag acctcggact cgtcctgctg cccgacacgc
     2281 aggtcttccg caagcggccg ccgcgtgagc gccgagtcgg cgggcgggcg ttgcagtcgt
     2341 tcgccgacct gcgcagcggc gattacatcg tccacgagga ccacggcgtc gggaagctgc
     2401 tcggcttcga gacgaaggag gtcgcgggcg tcacccgcga ctacctcttc ctcggcttcc
     2461 gcggcgacga ccggctctac gtcccgcacg agcagctcgg caaggtgtct cgctacatcg
     2521 gcgccgacaa ctccgcgccg gcgctctcga agctcggcgg caaggcctgg gacaatctca
     2581 agacccgtgc gagggagtcg gtgcgcgagc tcgccggcga gctgcttgcc ctctacgcgc
     2641 agcggcagca ggcgcccggc gtcgcgtacg acctgcgtca cgagtacctc gagcgtctgg
     2701 aagcggagtt cccgtaccgg gagacgcccg accagcaggc tgcgatcgaa gcggtgaagg
     2761 aagacctcga ggcgccgcgt ccgatggatc gcctggtgtg cggcgacgtc ggtttcggca
     2821 agaccgaggt cgccatccgc gccgcgttcg ccgtggccgt caacggcaag cagacgctga
     2881 tgctcgcgcc gacgacgatt ctcgccgagc aacactggca cacgttccgc gaccgctacc
     2941 gcgacttccc tgttcgtgtc gagatggtct cgcgcttccg ttcgccgcgg gaggtcaagc
     3001 aggtgctcgc ggacttccag gccggcaagg tggacgtcct gatcggcacg catcgcgtcc
     3061 tcagccgcga cgtcatcccc aaggaactcg gtctcgtcat cctcgacgag gagcagcgct
     3121 tcggcgtcgc ccagaaggag ttgctgcgtt ccctgcggct cgaggtcgac gtgctgacgc
     3181 tgagcgcgac gccgatcccg cgcacactgc acatgtccct ctccggcctc cgcgacatct
     3241 cgatcatcga gacgccgccg gaaggtcgcc gcccgatccg caccaccgtc agcgagtacg
     3301 acgaggaagc gatcaagctc gccctcgagc gcgaagtcga gcgcggcgga caggccttct
     3361 acctgcacaa ccgcgtcgaa acgatcgacg aggtgctgga gaagctgcaa cagcttgcgc
     3421 caaacctgcg tttcctcgca gcgcacggcc aaatgcgtga gcgggagctc gaggagaaga
     3481 tgcacggctt cctgcgcggc gacgcggacg tgctcgtgtc gacgacgatc atcgagtctg
     3541 gcctcgatat cccgcaggcg aacaccttga tcgtcgagcg cgccgaccag ctcggcctct
     3601 cgcagctcta ccagattcgc ggccgggtcg gccgctccga cgtgaccgcg cacgcgtacc
     3661 tcttctaccc ggacatgtct gagctgactc ccgaggcacg cgcgcgcctc gccacgctcg
     3721 cggacaacac cgagctcggc gccggctttg cgatcgcgat gcgcgatctc gagatccgtg
     3781 gggccggcaa cctgctcggc gacgagcagt cgggccacgt cgcagccctg ggcttcgagc
     3841 tgtacgtgga gatgctcgcc gaggccgttg ccgagctctc cggcgagcgc cgggtcgccg
     3901 cccgcccggt ccgcgtcgac gcgcgagtgg atgcctacgt tccggcggag tacatcgcgt
     3961 cggaggcgct caagatcgac ctccaccgcc ggctcgcgct gagcgagtcg gaggatgagt
     4021 tgcgcgagct gcaggccggg accgaagacc gcttcggccc gctgccggag cccgtcgaga
     4081 atctcttcct gattcaggag gcgaagctca agctggcgcg tgccggcgcc gactacttcg
     4141 tcttccgcgg cggcaaggcg agcgtcgggc agatggtgct tggctccgac gagctgcgcg
     4201 acctccggcg ccgcgtcgac acggcggtct acacgatcgc aaaccgtgag gtgtctttac
     4261 gggaggaggg tttcccgcag gcgctgcgac tggtcgatgc tatactggcc gcgcgtcagg
     4321 cagcgtgacg aatccgctgc aaatggcgca ttttccatga aacgatcctt tcttcttgca
     4381 ctcatctcgc tcgtgctcgt ggtcgccggc tgcggtggga cctcgaccgc gaccctgagt
     4441 tcgaacgacg cagccgtcgt cggttcagag caggtcacga aggaccagtt ccagtccctg
     4501 atggatcgcg cgaagacgag ctacgtcgcc cagaagcggc ccttcccgaa gccaggcagc
     4561 accgagtacg agcagctcaa gggccaggcg gtcacgttcc tgatccagcg ggcggagttc
     4621 gcgcaagagg ccggggcgat gggaatcaag atcacggacg acaaggtgaa caagcgcatc
     4681 gagcagctga agaagcagtt ctacggaggc agcgagcaac gctacgagaa gacgctcaag
     4741 cagcaaggtc tgaccgcgga tcaggcgaag gaagaggttc gcgcccagct gatctcggaa
     4801 gagctcttca agaaggtgac aggtgacgtc aaggtctcga aggacgaggt caaggcctac
     4861 tacaactcac acaagtcgca gtacgggcag ccgcagacgc gtgacgtgcg ccacatcctc
     4921 gtcacgaaga aggctctcgc cgactcgatt tacgcacagc tgaaggccgg cgcgaacttt
     4981 gcgaagctgg cgaagaagta ctcgaaggat cccggctcgg cctcgaacgg cggcaagctg
     5041 accatctcca agggacagac cgtccccgcc ttcgacaaga ccgccttcag cctgaagaag
     5101 ggtgagctgt cagcgccgat ccacacgcag tacggctacc acatcatcca agcgctctcc
     5161 gacgtcaagg cagcgcagac gacgaccctc agcaaggtgg agtcgtcgat caagcagcag
     5221 ctcgagcagc agaagaagaa ccaagtgatg acgaagtggg tcgacaagaa gaagaagagc
     5281 tactgcaagt cgggcatcaa gtaccaggtt gggtaccagc ccaacccgga cccctgcgcg
     5341 acgctcaccg gcacgaccac cacgacgtcc cagtaagtgg gtctcgccga ggcgctcgtc
     5401 gacctccagg agctgaccga gcggctgcgc cgcgagtgtc cctgggatcg cgagcaaacc
     5461 gagcgcacga tcgtgccgca cacagtcgag gaggcctatg aggtcgccga cgcggcgatc
     5521 ggcggggatg acgcgaagct cgcggacgag cttggcgacc tactgttcca ggtgtacttt
     5581 cttgccctgc tcctggcgga gaagggccaa ccggggttgg aggagattgc gcggggagtt
     5641 cacgagaaac tcgtccgccg ccacccgcat gttttcggcg gacctgagct tgactccccg
     5701 ggtgaagtcc gccgcacgtg ggagtccagt aagcgcgacc tggaaggtcg cgcaggcatc
     5761 ttccacgacg tgccggagtc gctgccagcg ttgctctacg cacggaaggt gcagcggcgg
     5821 gccgccgccg tcgacttcga gtatccggac accgcgggcg cgctcgcgga tctcgacgac
     5881 gaggtgcgcg agttgaggga ggcgctgacc gaggcaggcg agacccctcc cgagacagag
     5941 ccgccggcgc acgtgttcga ggaaatggga gacgtcctct tcgctgccgt caacgtcgcg
     6001 cggcggctga acgtcgatcc cgagctcgcc ctgcgcgcca tgtcgcgccg cttcgtcgag
     6061 cgggtggagc gtgcggaacg cactgccgcg gctgagggga agtcgttcgc cgagctggcc
     6121 ctggaggagc aagaccgcta cttcgatcgc gccaaggagg aattcaagtg agtgcttcct
     6181 cgatcgcgcg cgtgcacggg cgccagatcc tcgactcgcg tggcaatccg accgtcgaag
     6241 tggacgtcgt gctcgagtcg ggcgcgagcg ggcgcgcggc ggttccgtcc ggcgcgtcca
     6301 ccggccggtt cgaagccgtc gagttgcgcg acggcgacaa gcggtcctac cggggcaaga
     6361 gcgtcctcaa cgcggttcgc aacgtcgaga cggagatcgc gggcgcggtc acgggactcg
     6421 acgccggaga ccagcgcgcg ctcgacctga cgctgatcga gctcgacggg accaagaaca
     6481 agtcgcgact cggcgccaat gcgattctcg gcgtgtcgct cgcgacggcg aaggccggcg
     6541 cggctcacgc aggcgtctcg ctgttccgct ggatcggcgg ggccggcgcg catgtccttc
     6601 ccgtgccgat gatgaacgtg atcaacggcg gcgcccatgc gcagaactcg ctcgacctgc
     6661 aggagttcat ggtcgtccct gcaggcgcgg attcgttcgc cgaaggattg aggatcggcg
     6721 ccgagacctt ccacgcgctc aaggacgttc tgcacgagcg tggactgtca accggtgtcg
     6781 gcgacgaggg cggcttcgcc cccgagctgg actcgaccga ggccgcgatc gaggcggtgc
     6841 tcgaggcggc cgagcgagtc ggacaccgcg accgggtcgc gatcgcgctc gaccccgcct
     6901 cgaccgagtt ctaccgcgac ggcgcctatc acctcgaacg ccagggccag gtcatgagcg
     6961 cggaagagct gatcgatctc tatgcgtcac tcagtgaccg ctatccggtg gtctcgctcg
     7021 aagacgggct cgcagaggac gagtggggtg cgtggaagac gatgacggag cggctcggcg
     7081 accgcctgca gctggtcggc gacgacctct tcgtgacgaa cgtcgagcgg ttccagcgag
     7141 gcatcgacga gggtgtcgcg aatgcgatcc tggtcaaggt caaccagatc gggacgctga
     7201 ccgagtcgct cgacgcgatc gaactggccc gcgccaacgg ctatgccgtg gtgatctccc
     7261 accgttccgg ggagaccgag gacacgacca tcgcagacct ggtcgtcgcg acgggcgcgg
     7321 gccagatcaa gacgggcgcg ccctcgcgca cggaccgggt cgcgaagtac aaccagctgc
     7381 tccggatcga ggaggagctc ggtgacgccg ctgtgtatcc ggggtggcga gccttcccac
     7441 gacagcaacg ctgacgtacc ccggcgctaa cctgcatgca tggcttcgat gacgccgcgc
     7501 cggaccaaga tcgtcgcgac gatcggccct gcgtgctcga cgcccgagag gctacgggag
     7561 ctcgtcggag cggggatgga cgcggcgcgt ctcaacctct cacacgggac tcatgaagat
     7621 catgcggcga gagcgaagct cgtccgcgac gttcaggagg aggtcggccg gccgctcgcg
     7681 ctgatcggcg acctccaggg gccaaagttc cgcgtgggcg ggctcgacca gccgctcatc
     7741 ctcgttcagg gatcggaggt gacggtggtg acgaccggag acggcgccgg gaacggcgag
     7801 cttccggttg cgccggcggc catcggcgag gttctgcgcc ccgggcacga ggtgctgatc
     7861 gacgacggga tgatccggct ccgcgtcgac gccgtggagt ccggtcgttt ccgctgcgga
     7921 gtcgtcgtgg gcgggaccgt gacctcccac aagggcgtga accttcccgg tgtcccgatc
     7981 gcgatcccgt cgctgacgcg gaaggatctc gaagacctcg agttcgccct cgagctcggc
     8041 tgcgacttca tcgcgctgtc gttcgtccgg tccgccgccg atgtccgcga cctgaaggcg
     8101 ctgatcgagc aggagggatc gaccgctcac gtgatcgcaa agatcgagaa gtcggaggcg
     8161 gtcgacgcgc tcgacgacgt gctcgaagag gctgacgccg tgatggttgc ccgcggcgac
     8221 ctcggcgtcg agatcggccc cgagctcgtg ccgctcgtgc agaaacggat catctccaag
     8281 gcgctcgagc gcgggaagcc agtgatcacg gccacgcaga tgctcgagtc gatgatccac
     8341 catcccgagc ccacgcgcgc cgaggccagc gacgtcgcga acgccgtcct cgacggcacg
     8401 tccgcgatca tgctgtcggg cgagaccgcg atgggcgagt acccggtcca ggcggtgcag
     8461 acgatggacc gcatcgcccg cgcagtcgag ccgagcctcg gctaccgcca tcagattccc
     8521 gaagcgaccg aggacccgac gatcggcaac gcgatgtcca atgcagcctg cgacctcgca
     8581 gaggcgctcg gcgcgaaggc gatgctcgtg cccaccttca ccggacgtac ggcgtcggcg
     8641 gtcgcgaggc tgcgcccgcg ccgcccgatt gtcgcgctga cccacaagca gtggtccgtg
     8701 caccagatgg cgatcgagtg gggcgtgacg ccgatctgga tccccgagtg caaggacgtc
     8761 gaggatctct ggaaccggtc cctggacgcc gcgaaagagg caggactgat cgagtctggg
     8821 gacacggtgg tgatcaccgc tgggacggcc gtcaacatcc cgggctcgac gaacgtcatc
     8881 aaggtcgacg tagtcctcta aacggcgtcg ctaacgcgct tgctcgcagg agcaagccgg
     8941 gcttccgcga atcctgtggc tcatggctgc gcaggcttca cgtcgtccac ccctcgccac
     9001 gctcgcgcgg cggtggttcg ccgtcctgct ggtcgtggcg atcggctacg cgtactacca
     9061 ccccctgagg agctggctcg agacgcgtca cgagctctcc ctacggcgtg ccgaggtggg
     9121 gcggttgtca agtgagaggc gtggcttgca agaccggctc cagttctccg cgagcagcga
     9181 tgcgctcgca cgcgaggcgc gcaggctggg attcatccgc ccgggcgagc acctcttcat
     9241 cgtcaagggg atccaggcct ggaaaaaagc tcatcccgac cgctgaaatg ctgcgcattt
     9301 ccagcggggt agggtttcgg catcgcttcg ctcgcgaaag atgacgagga tcgcgccgcc
     9361 gtcgagcacc agctaggcag gccaccacgc gccttcagac gggtcgtcgt ccgctgtccc
     9421 ttcgggcatc ctgcagtaac tgagcagcac gcgttcgacg acaacggtga gccgtttccg
     9481 accacgttct ggctcacgtg tccccagctc gtggcggcgg tgtcacggct cgaagcgacc
     9541 ggcggcgtcg agcgctggac gcgcgcggcc gccgcggatc cccggctcgc tgcgagcctg
     9601 gagcgcgcac acgcagagca acgccggcta cggcccgagc tcgacctcgg ggtgggcggc
     9661 gcgcgcgcgg gggccggaac cctcaagtgt ctgcacgcac acgtggcatt cgcgctggcc
     9721 cgtcccggtt acgagttggg tgagcagatc ctcgccgagg tcgatccggc gtggccgtct
     9781 gtttgctgcg cgccgtaacg gataggttct gcgcccgtgt cggtagtggt cgacgtcgcg
     9841 agacgagagt gggaagacgg ctatcgccgc ttcgaagcgg cgtcgcgcga tcccatagct
     9901 gcggaccgcc tgaacgcgca actggaggtc gtgacgggag agctccgccg ccgtatcggg
     9961 cagaccttca cgctggagga gctggtggcc atctacgacc gcgcggaggc atgggtccgc
    10021 gacgttgtgt ccgagcacgc ggccacgccc ggctggcccc gcacgctcgc cctggtggag
    10081 gatgcggcct tccatctcta ccagcgcggc gcagtcgact acaagccgtg aacgctctca
    10141 ccccagtcga gcgcaacgaa cgccgccggc tgcgtcgccg tcaggagacg atccgctgga
    10201 gcatccgcgt gctcctgatc gtcctggtct tctctctcgg ggtcgctctt ggtcaggcga
    10261 tccaggacaa cccccggccg ggccggacgg tgacatctga ccgtacgctc cacgttccga
    10321 gcggcgggcc gcaaggttcg accattactc cctgaccatc gcgaagccgc cgactgggcg
    10381 gcgccgtctt gaagatgcgt cctacaggat taggcagatt tgcctctcgt ctccgctcag
    10441 gtgttaagaa ggcgcacctt tgggtagatt cgcggtatgg ctacgcgaga acccggtccc
    10501 aggcgggtcg ctgcgggcga gagcgactgg ctgacgcttg gccaggcggc gaagttcctg
    10561 ggcgtcgcgc agagcacgat cagaaaatgg tccgacgtcg gccgagttcc ggccttctac
    10621 acccccggcg ggcatcgccg gtacaagcgc ggtgacctcg acgccttcct cgaacgctcg
    10681 ggtcccggaa agccggcgcg cggcccgctc gtgcttctcg tggacgacga cgcgcaggtg
    10741 cgcgaagtcg tgcgcatcaa cctcgagatg gagggctacg ccgtccgcga ggcggcgaac
    10801 gcggaggacg ggctggccgc gctcgaggat gaggcgcccg acctgatcct gctcgacgtg
    10861 atgatgccgc aggtcgacgg ctgggagatg ttgcgacgtg tgcaggagcg gcacggtgtc
    10921 ggctcgatcc cggtggtcat gttcagcggc cagctcgatg cagggaacga agcagtgcag
    10981 cgtggcgcgc agggcttcgt cggcaagccg ttcgacctgc gcgcgctgat cgagcagacg
    11041 aagcagatcg ttccggcctg atctcgctcg accggcaact cgagcgctgg gtcgtccacc
    11101 atcgcgcggg ctggctgaat ccggtcttcg aagggctctc gtacgtcggc cggctggggc
    11161 tcatctggat cgtcatcgcg cttgtgctca gcgcggtcta tcgccgctgg ggcgtcttcg
    11221 ttctcacggt gatcgcgatc gcactcgcgg actggtcgtc gatggggttg aagtcgctcg
    11281 tcgaccgccc gcgcccacct ttgcgttatc cggaaccaaa gacgctcgtg cccctcccgc
    11341 atgacgcgtc gttcccgtcc ggccatgcag cgacgagctt cgctgcagcg acgatgctgt
    11401 cgctttcgtt tccgcgcttc gctccgttcc tctatgtgct cgcagccgcc atagcgttct
    11461 cgcgcgtgta cgtcggcgtg cattacccgc tcgacgtgat cggcggcgca gcgctcggcg
    11521 tgcttattgc gatagcgctg agggtgctga tgacccgcag agcacgcgga aggtcgagcg
    11581 ccctacaggc agaaccacca aggccggaag ctaagaccag gcgaagccgg gcttagctgg
    11641 aggcgtagtg atgcgggcgc ggacgcttta gcgcccgtgc ccggacggaa gggaacaatc
    11701 gccaagcacc aacaccattg gagtgcgcgg gctcggcccg cgcacgttca gcgcgaagcg
    11761 ccgtccccaa ttaccgagcg aaagcgaagc tttcgctcgg ttcaaacttc ttcgcctgct
    11821 cgtaagaggc cggcggcaat tacgagcagg gacgcgatca ggctgatgaa gatgccgatg
    11881 ccacgtccgc gccaaccgaa gaactcgtcg ggaacgctga tcagtcggat gagcacgaaa
    11941 atggtcgaga gcgcgccgag cccgatgatg accaggctct cgggaacggt tgcgggcagt
    12001 tcgatccccg tctcgcgcag gatgacgagc gccagcaccg ccagcccaat cacgaacacg
    12061 agcttgccca gcgccccggt atgccacccg atcacgctcg tcgtcggccc cggccccgag
    12121 ccgacgtacc agtcggtgaa cgccgagacg gccaggatca gcccggagac ccacgtgagc
    12181 cgcattccca ggccggagag gtcttgaaag gagcgggagg gcctgggctg agggatcgtc
    12241 cagtcgtcct ctgctggctc tggcggggga tagggctctg gcgtctgcac ggtatggcgc
    12301 tggagaagct gcggccgagg gattcacgcc gcaggaggta ctctaagggg gtctggatga
    12361 tggagggctt gcatacgtcg cgcgcggcac ctgacgagta cgtcgagttc agggcggccg
    12421 gcgacccggc caagggtgag ttccactgtt ccgagtgcgg ctatggggtc acgatcgtcc
    12481 gtgcactgcc gatctgcccc atgtgcggcg gcacgtcctg ggagcaaacc agttggagcc
    12541 cgttcggaag agcctcgagc ctgcagtaac caaatgctct agctaaaggc cgaagacctt
    12601 ctggacttcg tgccagatgg tcagcagcag gaacaacgag agcccgacca gcagtgtgaa
    12661 cgtgatgaag aagcgcaacc gtgcgttcga cttctcgcgt ttgcggcgca gccgcgcgtt
    12721 tcgccgagcc cggtgatagt ggtaggcacg gtcgatcgcc tcggggtcga ccggcgggtc
    12781 ctcgggtggc gccgcaacgt gccgtctggc tggctgggcc atccgaggga gtctagtgcg
    12841 gctctggccc agaactcgcc actcggacat acttcggcgg ggtcgggatg
//
LOCUS       DASVEU010000184         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1680437,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000184 DASVEU010000000
VERSION     DASVEU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680437"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF225_07570"
     CDS             complement(<1..648)
                     /locus_tag="VF225_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEX7145154.1"
                     /translation="MIAKYALRFAALGYLAVLLVAPVGFVFWRTFEHGVGPAWDAVTT
                     PDALHALKVTLILTAIAVPANTIFGVLCALAIVRHRYPGAGVVNAVVDLPLALSPVVV
                     GLALLLLYGRGGWLGGHAIVFALPGMVLATIFVSLPFVVREVVPVLREIGTEQEQAAA
                     TLGASAFQTFRRVTWPAIRYATAYGVVLTTARALGEFGAVSVVSGRLVGQTESLTL"
     gene            complement(638..1447)
                     /gene="cysT"
                     /locus_tag="VF225_07575"
     CDS             complement(638..1447)
                     /gene="cysT"
                     /locus_tag="VF225_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX7145155.1"
                     /translation="MAANADGLAAAPGARRSLAPGLSIGLSTLYLSVIVLLPLAALSW
                     KAHGWGAVSTPQATAALRLTLGLSLAVALVNAVAGTVIAWTLVRDRFVGQGFVNALVD
                     LPFALPTIVAGLTLLALYGPKSPVGINAAFTRWGLLLALLFVTLPFVVRTVQPVLFEL
                     DREMEDAAASLGAGRFTTFQRIILPSILPAVLSGVALAFARAIGEFGAVVLISGNLPF
                     KTEVVSVYVFGRLESGDSGGAAAASVIILLISFGVLLAIGALRRWVTRHDR"
     gene            complement(1437..2384)
                     /locus_tag="VF225_07580"
     CDS             complement(1437..2384)
                     /locus_tag="VF225_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7145156.1"
                     /translation="MLIALLLPALASGASKDTKLTLVAYSTPKDAYGQLISAFQKTQA
                     GNGVTFSQSYGASGEQSRAVAAGLDADIVAFSLEPDITSLVQKKLVAKSWNKDKYKGM
                     VTRSVVVFIVRDGNPKKLKTWNDLLKPGVQVITPNPFTSGGARWNVMAAYGGALRSGK
                     TPKQAVDYLGKLWKHVVAQPTSAREGLQTFLAGRGDVFLAYENEAVFAQKKGQPVQFV
                     IPKATILIENPIAVTSTSKHPTEAKVFVKFLRTLDAQKIFAQNGYRPVVPAAARGFNF
                     PTRPQLFRIKYVGGWAQVEKKFFDPKTGIMVKFIGSSGG"
     gene            2502..5219
                     /locus_tag="VF225_07585"
     CDS             2502..5219
                     /locus_tag="VF225_07585"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX7145157.1"
                     /translation="MSSSTLSAGGRSYEIFRLEGAERLPYTLRILLENALRHGEEGDA
                     KALRHWQATAEPAEEISFRPARVLLQDFTGVPAVVDLAGMRNAMADLGGDPSRINPQL
                     PAELVIDHSVQVDEYASARAMFRNAELEFERNRERYAFLRWGQTAFDNFKVVPPNTGI
                     VHQVNLEFLARVIETRDGQAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGEA
                     ISMLVPQVVGFKLTGAMPEGATATDLVLTVTQLLRAAGVVGKFVEYFGHGLVGLPLAD
                     RATIGNMSPEYGATCGFFPVDDETLKYLRLTGRDEERLALVEAYCKENALWHNPDYEP
                     EYSQVVELDLSTVEPSLAGPRRPQDRVPLREAKQSFVDALESFGVDYGNEHDEAVAES
                     FPASDSPATVEPGHGKLTPQVQVPVAGVAMLEQQTPMAVAVGGEEFELEHGSVVIAAI
                     TSCTNTSNPQVMIGAGLLAKKAVELGMHRKPWVKSSLAPGSKVVTEYFEQAGLDRYLD
                     QLGFQTVGYGCTTCIGNSGPLPEAISAAILEGDLVVCSVLSGNRNFEARIHPEVKANY
                     LASPPLVVAYALAGRMDVDLVNEPIGQDVDGDDVYLRDLWPSSDEIQETITRAVRGEM
                     FTRTYADVFTGDPAWRELPIPEGDLFAWDPQSTYVRQPPYFEGMSREPEEVEDVGGAR
                     CLVMIGDSVTTDHISPAGAIKPDSPAGKYLVEHGVARRDFNSYGSRRGNHEVMVRGTF
                     ANVRLRNLLVPGSEGTWTVHVPSGEEMTIYDASKRYLEEGTPLIVIAGKEYGSGSSRD
                     WAAKGPNLLGVRAVIAESYERIHRSNLLMMGILPLQFPEGETPSSLGLIGREEFAVEG
                     IENGSADEVTVRAGDKNFQARVRLDTPREREYFRHGGILQYVLRRLLQT"
     gene            complement(5283..5462)
                     /locus_tag="VF225_07590"
     CDS             complement(5283..5462)
                     /locus_tag="VF225_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145158.1"
                     /translation="MAKLPDADLQAREFIDRVVESHRKNGYGAKVPKKRYEEAVARVA
                     ASFRGLTDASKGRAA"
     gene            complement(5468..5767)
                     /locus_tag="VF225_07595"
     CDS             complement(5468..5767)
                     /locus_tag="VF225_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145159.1"
                     /translation="MALSLNKLKRLGDAGLVDLFEADRKLWTAMAKDAHAYTSKFVRG
                     SGNAVRPDDVVPTLTPALEVSDRLRTYLAERKLSQNYWYTWFAELIVDRLWSDLN"
     gene            complement(5758..6075)
                     /locus_tag="VF225_07600"
     CDS             complement(5758..6075)
                     /locus_tag="VF225_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145160.1"
                     /translation="MEVVPAIFGAAAGLAGLLLVFLGVSISAFQSFAGDTPAAATAGF
                     RRAGAWILGAFLLGLVTVAVSFAWLWSPHRALRDLAAVLFAVELAATASAAVTTARAV
                     LWR"
     gene            6121..>6522
                     /locus_tag="VF225_07605"
     CDS             6121..>6522
                     /locus_tag="VF225_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7145161.1"
                     /translation="MRLGKDGKVELIKKVPLFSKLSKKGLGEVAHIADELDLPKGKVM
                     AEEGDRGREFFVLLEGEADVTKGDKSINTMHEGDFFGEIALVTKMPRTASVTATTDVR
                     VLVITERDFGTLLKHSNEVGRGVAEALAERIA"
BASE COUNT         1211 a         2127 c         2189 g          995 t
ORIGIN      
        1 gagggtgagc gactccgtct gcccgaccag acgaccggag acgacgctca ccgcgccgaa
       61 ctcgccgagg gcgcgggccg tggtcagcac gaccccgtag gcggtcgcat agcggattgc
      121 cggccacgtc actcggcgga acgtctggaa ggccgacgcg cccagcgttg ccgccgcctg
      181 ttcctgctcc gtcccgatct cgcgcaacac cggcacgacc tcgcgcacga cgaagggcag
      241 cgagacgaag atcgtcgcca ggaccatccc aggcagcgcg aagacgattg cgtgcccgcc
      301 gagccagcca ccacgtccgt agagcagcag caaggcgagt ccgacgacga cgggcgacag
      361 cgcaagcggg agatcgacga ccgcgttgac gactcccgcg cccggatagc gatgacggac
      421 gatcgcgagc gcgcacaaca cgccgaaaat cgtgttggcg ggaactgcga ttgcagtgag
      481 gatgagcgtc accttcagcg cgtgcagggc gtccggagtc gtgaccgcgt cccaggcggg
      541 accgacgccg tgctcgaaag tgcgccagaa gacgaacccg acgggtgcaa cgagcagcac
      601 cgcaaggtag ccgagcgcag caaaccggag cgcgtactta gcgatcatgt ctcgttaccc
      661 agcggcggag ggcgccgatc gcgagcagca cgccgaacga gatcaaaagg atgatgaccg
      721 aagctgctgc ggcgccgccc gagtctccgc tctcgagccg gccgaacacg tacaccgata
      781 cgacctcggt cttgaacggg aggtttcccg agatgagcac gacagcaccg aactcgccga
      841 tggcgcgcgc gaacgcgagg gcgacgccgg acagaaccgc gggcaggatg ctcggcagga
      901 tgatgcgctg gaaagtggtg aagcgacccg cgcccagcga ggcggcggcg tcctccatct
      961 cgcgatcgag ctcgaagaga accggctgaa ccgttcgaac gacgaacggg agtgtcacga
     1021 agagcaatgc gagcaacagg ccccagcgcg tgaaggcggc gttgatcccg acgggcgact
     1081 tcgggccgta gagggcgagc aacgtcagac ccgcgacgat cgtgggaaga gcgaacggca
     1141 agtcgacgag ggcgttcaca aagccctggc cgacgaaccg gtcccggacc agcgtccagg
     1201 cgatcaccgt tccggcgacg gcgttcacga gcgcaacggc gagcgagagc cccaaggtga
     1261 gcctcaaggc agcagttgcc tggggcgtcg agaccgctcc ccagccgtgg gccttccagc
     1321 tcagcgcggc cagcggaagc aggacgatca cgctcaggta gagcgtgctc agaccaatgg
     1381 agaggcccgg cgcgagtgag cgccgggccc cgggtgctgc cgcaagtccg tcggcgttag
     1441 ccgccactcg agcctatgaa cttgaccatg attccggtct tcgggtcgaa aaacttcttc
     1501 tcgacctgcg cccagccgcc gacgtacttg atcctgaaca actgcggtcg agtcgggaag
     1561 ttgaagccgc gcgcggcggc cggtacgacg ggccggtagc cgttctgcgc gaagatcttc
     1621 tgcgcgtcga gcgtgcgcag gaacttcacg aacaccttcg cctccgttgg gtgcttcgag
     1681 gtcgacgtga cggcgatcgg gttctcgatc aagatcgtcg ccttcggaat cacgaactgg
     1741 acgggctgac ccttcttctg ggcgaacaca gcttcgttct cgtaggcgag gaagacgtca
     1801 ccgcggcccg cgagaaacgt ctgcagccct tcgcgcgcgc tggtcggctg cgcgacgacg
     1861 tgcttccaga gcttcccgag atagtcgacg gcctgcttcg gtgtcttgcc ggagcgaagt
     1921 gcaccgccgt aagcggccat cacgttccag cgggcgccgc ccgacgtgaa tgggttcggc
     1981 gtaatcacct gaacgcccgg cttcagcagg tcgttccagg tcttcagctt cttcgggttg
     2041 ccgtcacgca cgatgaagac gaccaccgag cgtgtgacca tccccttgta cttgtccttg
     2101 ttccagctct tcgcgacgag cttcttctgg acgagcgagg tgatgtccgg ctcgagggag
     2161 aacgcgacaa tgtccgcgtc cagcccggca gcgaccgccc ggctctgctc gcccgacgcg
     2221 ccgtaggact gggagaacgt gacgccgttc cccgcctgcg tcttctggaa cgccgagatc
     2281 aactgtccat acgcatcctt tggcgtcgaa taggccacga gggtcaactt cgtgtccttc
     2341 gaggcaccgg acgccagcgc cggcagaaga agggctatca gcaaagctag gctaattcgt
     2401 cgcatctcgt ctccttatta gtccactgaa tcggtggact ttagggacaa ggtgagacca
     2461 cgtcaaatgg gaacgggagg cggttccata caatcggtcg ggtgagctcc tccactctca
     2521 gcgcaggcgg gcgcagctac gagatcttcc ggctcgaagg cgccgagcga ctcccctaca
     2581 ccctgcggat cctgctcgag aacgcactcc ggcacggcga ggagggggac gccaaggcgc
     2641 tgcgccactg gcaggcgacg gccgaaccgg cggaagagat ctcgttccga cccgcccgcg
     2701 tcttgctcca ggacttcacc ggtgtgccgg cggtcgtcga tctcgcgggg atgcggaatg
     2761 cgatggccga tctcgggggc gacccgtcgc gaatcaatcc acagcttccc gccgagctcg
     2821 tcatcgacca ttcggtgcag gtggacgagt acgcgtcggc gcgcgcgatg ttccgcaacg
     2881 ccgagctcga gttcgaacgt aaccgcgagc gctacgcgtt cctgcgctgg ggacagacgg
     2941 cgttcgacaa cttcaaggtc gtcccgccga acacaggcat cgtccaccag gtcaacctcg
     3001 agtttctcgc acgcgtcatc gaaacgcgag acggccaggc gttcccggac acgctcgtcg
     3061 gcacggattc gcacacgacg atgatcaacg ggctcggcgt gctcggctgg ggagtcggcg
     3121 gcatcgaggc cgaggcggca atgctcggcg aagcgatctc gatgctcgtc ccccaggtcg
     3181 tcgggttcaa gctgacaggc gcgatgccgg agggcgcgac ggcgaccgat ctcgtcctga
     3241 cggtcacgca gctgctgcgc gccgcgggcg ttgtcggaaa gttcgtcgag tacttcggtc
     3301 acgggctcgt cgggctcccc ctcgcggacc gggcgacgat cgggaacatg tcgcccgagt
     3361 acggcgcaac ctgcggcttc ttcccggtcg acgacgagac gctgaagtat ctgcggctca
     3421 ctggacgaga cgaagagcgg ctggcccttg tcgaggcgta ctgcaaggag aacgcgctct
     3481 ggcacaaccc ggattacgag cccgagtact cgcaggtcgt cgagctcgac ctctccaccg
     3541 tggagccgtc ccttgctggg ccgcggcggc cgcaagaccg ggtgcctctg cgcgaggcga
     3601 agcaatcctt cgtcgacgcc ctcgagagct ttggcgtcga ctacggcaac gagcacgacg
     3661 aggcggtcgc ggagtcgttc ccagccagcg actcgcccgc gacggtcgag ccgggccacg
     3721 gcaagttgac cccgcaggtg caggtgcctg tcgcgggcgt cgcgatgctc gagcagcaga
     3781 ccccgatggc ggttgccgtc ggcggtgagg agttcgagct ggagcacggc tcagtcgtga
     3841 tcgcggcgat cacctcctgc acgaacacct cgaacccaca agtgatgatc ggggcgggcc
     3901 tcctggccaa gaaggccgtc gagctgggga tgcaccgcaa gccgtgggtc aagtcgagct
     3961 tggcgccggg ctcgaaggtc gtgacggagt acttcgagca ggcaggcctt gaccggtacc
     4021 tcgaccagct cggcttccag actgtgggct acgggtgcac gacgtgcatc gggaactcgg
     4081 gcccgctgcc ggaggcgatc tccgcggcga tcctcgaggg cgacctcgtc gtctgctctg
     4141 tgctctccgg gaaccggaac ttcgaggcgc gcatccatcc cgaggtgaag gcgaactacc
     4201 tcgcctcgcc gccgctcgtg gtggcgtacg cactcgccgg ccgcatggac gtcgatctcg
     4261 tcaacgagcc gatcggtcag gacgttgacg gcgacgacgt ctacctccgg gacctctggc
     4321 cgagctcgga cgagatccag gagacgatca cgcgggcggt gcgcggcgag atgttcacgc
     4381 gcacctatgc cgacgtgttc acaggcgatc ccgcctggcg ggagctgccg attcccgaag
     4441 gggacctctt cgcctgggat ccccagtcga cgtacgtcag gcagcccccg tacttcgagg
     4501 ggatgtctcg cgagcccgaa gaagtcgagg acgtcggcgg cgcacgctgt ctggtgatga
     4561 tcggcgactc agtgacgacg gatcacatct cgcctgccgg tgcgatcaag cccgactcgc
     4621 ctgcaggcaa gtatctggtg gagcacggcg tcgcgcgcag ggacttcaac tcctacggct
     4681 cgcgacgcgg caaccacgag gtgatggtgc ggggcacgtt cgccaacgtc cgcctgcgga
     4741 acctcctcgt gcctggctcc gaaggaacgt ggacggtcca cgtgccgagc ggcgaggaga
     4801 tgacgatcta cgacgcctcg aagcggtatc tcgaagaggg gacgccgctc atcgtcatcg
     4861 ccggcaagga atacggctcc ggctcgtcgc gggactgggc cgcgaagggc ccgaacctgc
     4921 tcggtgtgcg tgcagtgatc gcggagtcgt acgagcggat ccaccgctcg aacctgctga
     4981 tgatggggat cctgccgctg cagttcccgg agggtgagac gccgtcgagt ttgggcctga
     5041 taggacgcga ggagttcgcg gtcgaaggga tcgagaacgg cagcgcagac gaggtgacgg
     5101 tgcgcgccgg cgacaagaac ttccaggcac gcgtgcgtct agacacaccg cgcgagcgcg
     5161 agtacttccg ccacggcgga atcctccagt acgtgctgcg gcgtctcctc cagacctgac
     5221 ttgtcggcct ccgacacgac cagaacggca agggtctgac cccagccgtt caagacctgc
     5281 gactaggcgg ctcggccttt tgaggcgtca gtcaggccgc ggaaacttgc tgcgactctc
     5341 gcgaccgctt cctcgtagcg cttcttcggg acctttgcgc catagccgtt cttgcgatgg
     5401 gactccacca cccggtcgat gaactctctc gcctgcaggt ccgcatccgg cagcttcgcc
     5461 acggccgtca gtttaggtca ctccagagac ggtcgacgat cagctccgcg aaccacgtgt
     5521 accagtagtt ctgggagagc tttcgctccg ccaagtacgt ccgcagacga tccgagacct
     5581 ccagcgcagg cgtgagcgtc ggaacgacat cgtcgggcct aaccgcgttg cccgagccgc
     5641 gcacgaactt gctcgtgtag gcgtgggcgt ccttcgccat cgccgtccag agcttgcggt
     5701 ccgcctcgaa gaggtcgacg agcccggcgt cgcccaggcg cttcagcttg ttgaggctca
     5761 gcgccacagg actgcacgcg cggtcgtgac ggcggccgaa gctgtcgctg cgagctctac
     5821 ggcaaacagc accgcggcca ggtcgcgcag cgcccggtgt ggcgaccaca gccaggcgaa
     5881 cgagacggcg acggtgacca ggccgagaag gaacgcgccc aggatccacg cgcctgcgcg
     5941 ccggaaacct gccgtcgccg ccgccggcgt gtcgccggcg aacgactgga aggccgagat
     6001 cgagacgccg aggaagacca gcagcaggcc ggccaggccg gctgcggccc cgaaaatcgc
     6061 cggaacgacc tccattcgtc aaaagtagcc aagtcttcgg aaggcagtta cagtcgccac
     6121 atgcgactgg gcaaggacgg aaaggtcgag ctgatcaaga aggtgccgct cttctcgaag
     6181 ctctcgaaga aggggctcgg ggaggtggcc cacatcgccg acgagctcga ccttcccaaa
     6241 ggcaaggtga tggccgagga aggcgatcgc ggccgcgagt tcttcgtcct gctcgagggc
     6301 gaggcggacg tcacgaaggg cgacaagagc atcaacacga tgcacgaggg cgacttcttc
     6361 ggtgagatcg cgctcgtcac caagatgccg cgcacggcaa gcgtcacggc gactaccgac
     6421 gtccgcgttc tcgtcatcac ggaacgggac ttcggcacgc tgctcaagca ttcgaacgaa
     6481 gtcggccgcg gcgtcgcgga ggcgctggcc gagcgcattg cc
//
LOCUS       DASVEU010000185         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1680463,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000185 DASVEU010000000
VERSION     DASVEU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680463"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..621)
                     /locus_tag="VF225_07610"
     CDS             complement(106..621)
                     /locus_tag="VF225_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7145162.1"
                     /translation="MAGNPSPPDYELADRMALTGPSQVKAISHPLRTTILGLLHERAA
                     TVTELAIAVERPKSTVAYHVNVLTEAGLVQVVRTRRVRAIEERLYGRTARMFYVGVEQ
                     RPDGDDLPRDFNDFEVAAQESGAAYRDGKLWGFIRHARISEAQASEFWERMAELVAAF
                     ERLPRSGETVY"
     gene            686..1282
                     /locus_tag="VF225_07615"
     CDS             686..1282
                     /locus_tag="VF225_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7145163.1"
                     /translation="MTEVVLYHHVQGLTEGVRSFSDELRQAGHTVHTPDLFDGRRFDT
                     IEEGMAFAGGAGFGALAERGVAAAQRINPEAVYAGFSFGVMVAQQLAQTRPGARGALL
                     MYSCLPVSEFGDAWPEGVPVQVHGKDADPFFDEDLEAARALVDSTDRAELFLYPGAEH
                     LFADSSLPAYDDAAAALLTERVLAFLDAVEADNTPIKD"
     gene            1414..1809
                     /locus_tag="VF225_07620"
     CDS             1414..1809
                     /locus_tag="VF225_07620"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7145164.1"
                     /translation="MATRKAACSCGQLRLIADGDPIRVSICHCHECQRRTGSVFGVQA
                     RFDAEQLRTEGRSSEYVRISDDGEERIFSFCPECGGTVFFKFPAMPEMIAVPVGVFAD
                     PDFPVPIRSIYETRRHPWVQLPDGIEREA"
BASE COUNT          327 a          703 c          715 g          373 t
ORIGIN      
        1 gggaatgtcc cttgaactcg atcctgctct agtgccgcct tcgacttcct agtcgccgcc
       61 ggccgggagg gtcggatggt cggtgggata gacgccgacc gcgaatcaat agacggtttc
      121 gcccgaccgc gggagccgct cgaacgccgc caccagctcc gccatccgct cccagaactc
      181 cgacgcctgc gcttccgaga tgcgggcgtg ccgaatgaaa ccccacagct tcccgtcacg
      241 gtacgccgcg cccgactcct gagcggcgac ctcgaaatcg ttgaagtccc gaggcagatc
      301 gtcgccatcc ggcctctgct cgactccgac gtaaaacatc cgcgcggtgc ggccgtagag
      361 gcgctcctcg atggcgcgca cccgccgggt cctcaccacc tgcaccagtc cggcctcggt
      421 gaggacattg acgtggtagg cgaccgtgct cttcggccgc tcgaccgcaa tcgccagctc
      481 ggtcaccgtg gccgcccgct cgtggagcag gcccaggatc gttgttcgga ggggatggct
      541 gatcgccttc acctgtgagg gcccggtgag ggccattcgg tcggcgagct cgtagtccgg
      601 gggagacggg ttgccagcca tggccaaaca gactaacatt gcagaatgtt ccaactttgt
      661 ggatcaatag tcaggaggat ctgagatgac cgaggttgtc ctgtaccacc acgtccaggg
      721 gctgaccgag ggagtgcggt cgttctccga cgagttgcgg caggcggggc acaccgtgca
      781 cacccccgac ctgttcgacg ggcgcaggtt cgacacgatc gaggaaggca tggccttcgc
      841 aggcggagcg gggttcggcg cgctggccga gcgcggggtc gccgccgccc agcggatcaa
      901 ccccgaggcc gtctacgccg gcttctcctt cggggtcatg gtcgcccagc agctcgcgca
      961 gacccggccc ggcgcccgcg gggccttgct catgtactcg tgcctcccgg tctcggagtt
     1021 cggggacgcg tggcccgagg gcgtcccggt gcaggtgcac ggcaaggacg ccgacccgtt
     1081 cttcgacgag gacctcgagg ccgcgcgggc cctggtcgac tcgaccgacc gggccgagct
     1141 gttcctctac ccgggggcgg aacacctctt cgccgactcc tcgctcccgg cgtacgacga
     1201 cgccgcggct gcgttgctca cggagcgcgt gctggcgttc ctcgacgcgg tcgaagccga
     1261 caacacgcca atcaaggact gatcggcgca ggtggcgtca cgcggctcgt ctggccgcgg
     1321 gaacagcgcg agtagcgccg cctgcgccag cgttgcggga tgtcccccga agcgctgtcc
     1381 gcgagttcgg ctaccgcctc taggatccac cggatggcca cgcgcaaggc agcgtgcagc
     1441 tgcggacagc ttcgcctgat cgcggatggc gatcccattc gggtttcgat ttgtcactgc
     1501 cacgagtgtc agaggcgaac cggcagcgtc ttcggcgtcc aagcacgctt cgatgcggag
     1561 cagttgcgca ccgaaggtcg cagcagcgaa tacgtacgca tctccgatga cggggaagaa
     1621 cggatcttct cgttctgtcc ggagtgcggc gggacagtct tcttcaaatt tccggcgatg
     1681 ccggagatga tcgccgttcc cgtcggcgtg ttcgcggatc ccgattttcc cgtgccgatt
     1741 cggtcgatct acgagacgcg ccggcatccc tgggtgcagc tgccggacgg catcgagcgc
     1801 gaagcctgag agtcagccgc tctcgcgtgc gcgagagcga caaaaccgca gtcttttaga
     1861 aagagttgtg tgggcgagtg ttcgggagtg cccccgaggc gtttcgtccc cggtcgcaac
     1921 tacgcgaagg tctgtgggtg atgtcgcctg tgacggcgca tacttcgcga gccggtcgac
     1981 gctcacgcgg ctagtgtcct gcgggtgaag gtcaccgctg aggcggcgcg tcgctttctc
     2041 gttgcgcggc acttcctcgc gcctgcgcgg tctctcgcgg gaggcctcga cggggtgctc
     2101 gaggtcttcc ggaagttc
//
LOCUS       DASVEU010000186         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1096857,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000186 DASVEU010000000
VERSION     DASVEU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096857"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF225_07625"
     CDS             <1..232
                     /locus_tag="VF225_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7145165.1"
                     /translation="GYPDGIAGEDIPLAARIVHAAAAYVGMRSERPYRAALAPEAAVA
                     ALESGSGTQFDPAVARAVAAVARTRLETERAA"
     gene            271..342
                     /locus_tag="VF225_07630"
     tRNA            271..342
                     /locus_tag="VF225_07630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Arg,seq:tcg)
     gene            543..1202
                     /locus_tag="VF225_07635"
     CDS             543..1202
                     /locus_tag="VF225_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7145166.1"
                     /translation="MHRGTNGDLRSDLRRAPPLPKSSASPPTSLSRRSGRWSRDEPES
                     STVGLIVAVVSLAVMPTLAYAKQQTGRARRALQADAVETWVCAYLSLALLVGVGANLA
                     LGWWWADPVAALTMLPIIAWQGWETLEEARKAQSDSTKLPVGRGVRVRARAASRGRRA
                     APRGHPRRRIAARAIVLDEPVRSGVLGGVRVLPFVAVTEGVDSGAPEKTFDVSLSGLR
                     R"
     gene            complement(1177..1608)
                     /locus_tag="VF225_07640"
     CDS             complement(1177..1608)
                     /locus_tag="VF225_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145167.1"
                     /translation="MSVSSPVLTGPGGGRTVESGHGSSAELKIAGEQSGGEWAVVEWR
                     VRAGDEPPIHTHTREDETIYVLEGAITALVADQRIEVEAGSYAALPKNVPHGLSVRGE
                     EARLLVTLEPAGAEYLLVPRDENDADPSRFGIVLNGAAPTG"
     gene            1889..2410
                     /gene="msrA"
                     /locus_tag="VF225_07645"
     CDS             1889..2410
                     /gene="msrA"
                     /locus_tag="VF225_07645"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7145168.1"
                     /translation="MSDGVEKAILAGGCFWGMEDLFRKRPGVTSTRVGYTGGDVLNAT
                     YRNHGTHAEAIEIVFDPERTSYRELLEFFFQIHDPTTVNRQGNDIGPSYRSAIFYLDE
                     QQRRVAEETIADVDTSGRWPGEVVTEVTPAGPFWEAEPEHQNYLERYPHGYTCHFVRS
                     GWVLPERTKTATG"
BASE COUNT          428 a          856 c          886 g          413 t
ORIGIN      
        1 cggctacccg gacggcatcg ccggcgagga catccccctc gcagcgcgca tcgtccacgc
       61 ggccgcagcg tacgtcggga tgcgctcgga gcgtccctat cgagcggcgc tcgctcccga
      121 ggccgcggtg gccgcgctcg agagcggctc cgggacgcag ttcgacccgg cggtcgcccg
      181 cgccgtcgcc gcggtcgccc gcacgcggct cgagaccgaa cgcgcggcct agggtcgagc
      241 ggcgagacgg cgggcatact gcccgcacgg ggccccgtag ctcagtggat agagcggcag
      301 acttcgaatc tgcgtgcgga ggttcgactc ctcccggggc cataaaagac ctgcaaaata
      361 gcactatctg gtacaccgtt taggcaacga ttgtgacacg gacggcgccg agcgtaccga
      421 gagagacgga aacgtcctgc aagtcgcgga aacgggcgga aggcgcgcac gcagattttt
      481 gatctgcggc tcgatctcgc ggtggggaag aaaatgcctg tgagcaggag atttctgtcc
      541 gcgtgcaccg cggcaccaac ggtgacttac gcagcgactt acggcgcgca cccccgttgc
      601 caaagagctc ggcttcgccg cctacatcgc tttcgaggcg atcggggcgc tggtcgagag
      661 acgagcccga gagctcgacg gtcgggctca tcgtcgccgt ggtgtcgctc gcggtcatgc
      721 cgacgctcgc ctacgccaag cagcagacgg ggcgcgcgcg tcgcgcgcta caggcggacg
      781 ccgtcgagac ctgggtgtgc gcgtacctgt cgctcgcgct cctcgtcgga gtcggcgcca
      841 acctcgccct cggctggtgg tgggccgacc cagtcgcggc gctcacgatg ctgcccatca
      901 tcgcctggca gggatgggag acgctagagg aggcccgtaa agcgcagtcc gacagcacca
      961 agctcccagt tggccggggc gttcgcgtta gggctcgagc cgccagccgc gggcgaagag
     1021 cagcgccgcg ggggcacccg agacgccgca tcgccgcacg tgcaatcgtc ttggacgagc
     1081 ccgtacggag cggcgtcttg ggcggcgtgc gcgtgttgcc atttgtcgcg gtcacggagg
     1141 gcgttgactc tggcgccccg gagaagactt tcgacgtcag cctgtcgggg ctgcgccgtt
     1201 gaggacgatg ccgaagcggc tcggatcggc gtcgttctcg tcgcgcggca caagaagata
     1261 ctccgctccg gcgggctcga gcgtgacgag caggcgtgcc tcctcgccgc gaacgctcag
     1321 gccgtgcggc acgttcttcg gaagcgcggc gtaggagccg gcttcgacct cgattcgctg
     1381 gtcggcgacg agtgcggtga tcgcgccctc gagaacgtag atcgtctcgt cctcgcgcgt
     1441 gtgcgtgtgg atcggtggct cgtcgcccgc gcggacgcgc cactcgacga cggcccactc
     1501 cccgccggac tgctcaccgg cgatcttcag ctcggcgctg gagccgtggc cggactcgac
     1561 ggtgcgacct ccgccggggc ccgtcaaaac aggactactc acagacatct gccactcctt
     1621 tgtgatcgtc cgcgttcgaa caacgcgggc tgtcgagatc aatctgccgg ccgccaggac
     1681 gcgcatggct accgcgaggt gcgcagcggg ggcgtgcgtt ccttgcttgt tctgagccgg
     1741 agatcgagag gcgtctctcg ggatcgaggc acgccaaccg tgggccgtgg ccgggcacgg
     1801 cgtcattccc ttgacacgac ggtatggcct cgggctacga gcttgctggc aggctggtga
     1861 cgaatcgcgt gagcgaggag gtcggtacgt gagcgacggc gttgagaaag cgatccttgc
     1921 cggaggatgt ttctggggga tggaggatct cttccggaag cgacccggcg tcacttcgac
     1981 ccgggtcggc tacaccggcg gcgatgttct gaacgcgacg taccgcaacc acggcacgca
     2041 cgccgaggcg atcgagatcg tcttcgatcc cgagcgaacc tcctaccgcg agctgctcga
     2101 gttcttcttc cagatccacg atccaaccac ggtcaaccgt caaggcaacg acatcgggcc
     2161 gagctaccgc tcggcgatct tctatctcga tgagcagcag cgccgcgtcg ccgaggagac
     2221 gatcgccgac gtcgacacct cgggccgctg gcccggggag gtcgtgaccg aggtgacccc
     2281 cgccgggccg ttctgggaag ccgagcccga gcatcagaac tacctcgagc gatacccgca
     2341 cggctacacc tgccatttcg ttcgttcggg ctgggttctg ccagaacgga caaaaacggc
     2401 caccggctag gcacaagcct cacactccgg ccgttcccca tcgcagggtg ctcgggtact
     2461 gcggctatcc cttttgcaca ccgaaatccc ctcggacgac gaggtcgatg acaggctggg
     2521 gatatgcgct ggctgatcga cgttcgcgcg aacagagaga cgcgatggac tcaccgacgt
     2581 tcg
//
LOCUS       DASVEU010000187         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_125988,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000187 DASVEU010000000
VERSION     DASVEU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125988"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..747)
                     /locus_tag="VF225_07650"
     CDS             complement(94..747)
                     /locus_tag="VF225_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX7145169.1"
                     /translation="MESGAYTQSGDQYPVQFSVEYPDRDLDRLMTAFRIIVAIPILIV
                     LGAIGGGWGSWGGGGNGEWVAAGGGGILFFPTLLMIVFRQKYPRWWYDWNLELLRFQN
                     RVAVYLGLMDDRYPSTDERQSVSLEFPYPDAKQGLNRWLPLVKWFLAIPHYIVLVFLG
                     LAAIVCVIIAWFAILFTGRYPKELFDFVVGVMRWGNRVAGYAFVLVTDQYPPFRLSA"
     gene            complement(789..1727)
                     /locus_tag="VF225_07655"
     CDS             complement(789..1727)
                     /locus_tag="VF225_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX7145170.1"
                     /translation="MQNARALACPASLKGVLSARDAAAALAEGLRSWAEVDELPVADG
                     GEGTLDVLHSALGGEWREAEVPDAFGRSRVARWLQLPSGVAVVEAAEAIPLDQERLDP
                     FAASSRGLAEVIRAVGRPRELLVCLGGTANVDGGAGLLAELDELPAPTRVACDVDVPL
                     VDAARLFSAQKGASPADAAELEARLVAMPQLAAYADVPGAGAAGGLGAAFASLGAELL
                     PGAPLVLDLVGFDPTGYDLVVTGEGTVDLTTVRGKAPGEVARRCAAAGVRCVVFGGRI
                     GEALPELETIALSGDPNQAERDLVALGKFLRDSEAG"
     gene            complement(1693..>2540)
                     /locus_tag="VF225_07660"
     CDS             complement(1693..>2540)
                     /locus_tag="VF225_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7145171.1"
                     /translation="VNAFERPHPSFLLVFSAFSVGLFANAILPGRIGELARVAVLNRR
                     MPQRSAWARLLGTVFAHRVFDLVAVIVLVVYVTFTAHIPSWAVTSLIAVVVVGVILFT
                     LAVLGARNDRYSGIEDGLGAVRKVVRLARYGLGVMRRPASATLALLFQLLGWTCQLLA
                     VYTAMRAFGIHQGLPAAGLVLVLMNVATIFPLWPGNIGLVQAAVALPLFTYYGVDKAR
                     GIAFGFGLQAIEASVGISVGLIFLAREGLSIAMLRVMPDAAQAETPEEEPAADAERPR
                     ARMSG"
BASE COUNT          486 a          904 c          821 g          329 t
ORIGIN      
        1 agctctacaa gctctgcgtc ggccggctcg acgcggcgca gggcaagatc gaggagctgg
       61 cccggcgggc cgaacagacg cgaccgtagg tcgctatgcg ctcaaccgaa acggcgggta
      121 ctggtcggtc acgaggacga aggcgtagcc agcaacccga ttgccccagc gcatgacgcc
      181 cacgacgaag tcgaagagct ccttcggata gcggccggtg aagaggatcg cgaaccacgc
      241 gatgatcacg cacacgatcg ccgcgagacc gaggaagacc agcacgatgt agtgggggat
      301 cgcgaggaac cacttgacga gcggaagcca tcggttcagg ccttgcttcg cgtccggata
      361 cggaaactcg agcgagaccg actggcgctc gtccgtcgac gggtagcggt cgtccatcaa
      421 accgagatac accgcaaccc tgttttggaa ccgcaacagc tcgaggttcc agtcgtacca
      481 ccagcgcgga tacttctgac gaaacacgat catcagcagc gtcgggaaga acaggatccc
      541 gccgcctccc gctgcgaccc actcaccgtt cccgccgccg ccccagctgc cccagccgcc
      601 gccgatcgcg cccagcacga tcaggatcgg gatcgcaacg atgattctga atgccgtcat
      661 cagccggtcg agatcccggt ccggatactc gaccgagaac tgcaccggat actgatcccc
      721 actttgtgtg tacgcgcctg actccatttg cgcctgccct ccgtgtgatg aatcgagacc
      781 aggcgacact accctgcttc cgagtcgcga agaaatttcc cgagggcgac gagatctctt
      841 tcggcttggt tcgggtcacc ggatagggca atcgtttcga gctccggcag agcctcgccg
      901 atgcgaccgc cgaacacgac gcaacgtact cccgccgccg cgcagcgccg cgcgacctcg
      961 ccaggagctt tccctcgcac cgtcgtgagg tccaccgtcc cctcgcctgt gaccacgagg
     1021 tcgtacccgg ttgggtcgaa gccgacgaga tcgaggacga gcggcgcacc gggcaaaagc
     1081 tccgcgccaa gagaagcgaa ggccgcaccc aggccgccgg cggcgcccgc ccccggcacg
     1141 tccgcgtacg ccgcgagctg cggcattgcg acgagccgtg cctcgagctc ggcggcgtca
     1201 gcaggcgagg cgcctttctg cgccgagaac agacgcgccg cgtcgacgag agggacgtcg
     1261 acgtcgcagg cgacacgcgt cggcgcaggc agttcatcaa gctccgccag cagacccgcg
     1321 ccgccgtcga cgttggccgt cccgccgagg cacacgagca gctcgcgcgg ccgtccgaca
     1381 gcgcgaatca cctcggcgag gccgcgactg gaggccgcga acggatcgag ccgctcctgg
     1441 tcgagcggga tcgcctcggc ggcctcgacc acggccaccc cgctcgggag ttggagccag
     1501 cgcgccacgc gcgaacgacc gaacgcatcc ggcacctcgg cctcacgcca ctcgcccccg
     1561 agcgccgagt gcagcacgtc gagcgttccc tccccgccgt cggcgacggg aagctcgtcg
     1621 acctcggccc acgagcgcaa tccctccgcc agcgctgcag ccgcgtcgcg cgccgacaga
     1681 acgcctttga ggctagccgg acatgcgagc gcgcgggcgt tctgcatccg cggccggctc
     1741 ctcctccggg gtctccgcct gcgccgcgtc cggcatcacc ctgagcatcg cgatggaaag
     1801 gccctcgcgc gcgaggaaga tcaggccgac gctgatcccg accgacgcct cgatcgcctg
     1861 gaggccgaac ccgaacgcga tcccgcgcgc cttgtccacc ccgtagtagg tgaacagcgg
     1921 cagtgcgacc gccgcctgca cgagcccgat attcccaggc cagagaggga agatcgtcgc
     1981 gacgttcatc agcacgagca cgaggccggc ggccgggagc ccctggtgga ttccgaatgc
     2041 gcgcatcgcc gtgtagacgg ccaacagctg acacgtccag ccgagtagct gaaagagcag
     2101 cgcgagcgtg gcagacgcgg gccggcgcat gacgccgagc ccgtaccgcg cgagccgcac
     2161 caccttgcgc accgcgccga gaccgtcttc gatccccgag taacggtcgt tgcgggcacc
     2221 gagcaccgcg agggtgaaga ggatgacgcc gacgacgacg acggcgatca gactcgtgac
     2281 ggcccagctc ggaatgtgtg cggtgaacgt cacgtacacg accaggacga tgacggcgac
     2341 cagatcgaaa acgcggtgcg cgaacaccgt gccgagcaag cgcgcccagg cgctgcgctg
     2401 aggcatgcgc ctgttgagta ccgcaaccct cgcgagctcg ccgatccgtc ccggcaggat
     2461 ggcgttcgcg aacagtccga cggagaaggc tgagaagacg agcaggaagc tcgggtgcgg
     2521 tctctcgaac gcattcacga
//
LOCUS       DASVEU010000188         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_126239,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000188 DASVEU010000000
VERSION     DASVEU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126239"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF225_07665"
     CDS             complement(<1..436)
                     /locus_tag="VF225_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7145172.1"
                     /translation="MADGVQVEKRRLDASENDALIGMARMGADAFGYEADLRIDRQLA
                     QLLRLRVSQLNNCTYCLSLHYEATRDAGIPRAKIDTLTAWWETELFSEAEQAALRYAE
                     ALTRAADTDRDAAFQRFHDALAENFSMEQMLEIVGVVVNMNVW"
     gene            1495..1842
                     /locus_tag="VF225_07670"
     CDS             1495..1842
                     /locus_tag="VF225_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145173.1"
                     /translation="MSPLNSTEHGDPHRARTKLGSFAIGLIHGMGGSAGVGILLVASI
                     ESQLLGVISLGALAIFTGRSMTILSRAFGAALASRPLRSSLTAWRLVSESRASRSRSG
                     TELPPGPLAPYPF"
     gene            1952..>2371
                     /gene="rpiA"
                     /locus_tag="VF225_07675"
     CDS             1952..>2371
                     /gene="rpiA"
                     /locus_tag="VF225_07675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEX7145174.1"
                     /translation="MSHLASEATSELEKRTAAATAAALVESGMTLGLGTGSTVAYLLP
                     ILASRRLRLRCVATSVATERAARRAGLRVEPFDALDELDLAVDGADQVHRSGWLVKGG
                     GGAHTREKIVAAAARRFIVIASSDKLVDELRPPVPLEL"
BASE COUNT          359 a          834 c          768 g          410 t
ORIGIN      
        1 tccagacgtt catgttgacg acgacgccga cgatctcgag catctgctcc atgctgaagt
       61 tctcggcgag cgcgtcgtgg aagcgctgga acgctgcgtc tcggtcggtg tcagccgcac
      121 gggttagagc ctcggcgtag cggagcgccg cctgctcggc ctcggagaaa agctcggtct
      181 cccaccacgc ggtcagggtg tcgatcttgg cccgagggat gcccgcatcg cgcgtcgcct
      241 cgtagtgcag gctgaggcag tacgtgcagt tgttgagctg cgaaactcgc aggcgcagga
      301 gttgggcgag ttggcggtcg atccgcaggt ctgcctcgta cccgaacgca tccgcgccca
      361 tccgcgccat cccgatcagt gcatcgttct cgctcgcgtc gagccggcgc ttctccacct
      421 ggacaccgtc agccatcttg cttcctttcg tgctcaggag acaactcccg tcgtgcagtt
      481 gcggtgacgg gccgggggcg actctgtatc gagcgccgac cgaaccgtag cggcgacgcg
      541 gccgcccagg tggaagtgct cgaaggcgag cgggcatcac gagcactgcg catctgcgcc
      601 gaagcctctc gatgtggaaa tcgctggttc tgagcgagtc gacgaactcg agcatgtagt
      661 tggggaggtc gcccgcacgc tgggccgcgg caagcgctcg atcagcttca cccggcctcg
      721 actccttgac cgcgcctctc agcagccagc ggccgaccgg ccgcgcggga cgggctcgag
      781 agctgtgctg agctccgggc tgcgccagcg ctctggcttg cagatgggaa cgtcagctca
      841 cccaggtgcg acgcgcgcac caaagagcac atgctcgccc ccggcctcgt gccggcagtg
      901 tgctcctctc acagcgtcat cggaggaagg gagacgctct tcgatgagga cgtgcttcat
      961 caccaacgcg atcgtcgctg tcgaccgggt tcctggtcca acagcatgca cagccgcggc
     1021 ccgcatgctg gccacgtatc ccggtcggac cgaatgatcc accgaaggag aacccatgtc
     1081 gcgcttgatc ggatactcgg ccctgctcgc cgccgccaca ctggcaatca tcgaaatcgt
     1141 catcggctag tttcggcgac ggtctcgcgc ccgcacctca atccccgcac atccctgcac
     1201 atccgcggga atgtgctgga gctggcgtct gggcgcggtc ccccaggcag tcctgcggtc
     1261 gacagccctg ctctcgccgc tctcgagctc cgcgacttcc ttgaccacgc cgtcacgcag
     1321 gcgctgctcc atcggcgcgc cacgaggcac cgcgggaacg accgcgtgcg ctggcctcag
     1381 ctaagagccg agtcgtccgc tcgcgttccc ggaatcccgt cctgccgggc ccacgttggg
     1441 cgcgtggcca cgggctcaga ccgggtcatg tccttccaac acaggaggta cgtaatgtct
     1501 ccgctcaaca gcaccgagca cggcgatcca catcgcgccc ggacgaagct gggctcattc
     1561 gccatcggcc tcatacacgg aatgggcggc agcgccggcg tcggaatcct gctcgtcgcg
     1621 tcgatcgaat cgcagctgct cggcgtgatc tctctgggcg cgctggccat cttcaccggc
     1681 cggtcgatga cgatcctgtc gagggctttt ggcgcggcgc tcgccagccg gccgctcagg
     1741 tcgtcactga ccgcgtggcg cctggtctcg gagtcgcgag cttcgcgttc gcgatctggt
     1801 acggagctgc cgcctggacc cctcgccccc tatccgttct gaacgcagct cgtgcacagc
     1861 tcgcgaggcg ctcgagcccg tacggcacgc gcatgacctc cccgaggatc attcggttct
     1921 tcttcgtcgc ccatccggcg gtgaaagcgc gatgagtcac ctcgcgtcag aggcaacctc
     1981 cgagctcgag aagcggaccg ccgcggcgac ggcggctgcg ctcgtcgaga gcgggatgac
     2041 gctgggactg gggacgggct cgacggtcgc gtacctgctg cccatcctgg cgtcgcgaag
     2101 gctccgcctc cgctgcgtcg cgacgtcagt cgccacggaa cgtgctgctc gccgcgccgg
     2161 tcttcgtgtc gagcccttcg acgccctcga cgagctcgac ctcgcggtcg acggcgcgga
     2221 tcaggtccac cgttccggct ggctcgtgaa gggaggcggc ggggcacaca cgcgtgagaa
     2281 gatcgtcgcc gctgcggccc ggcggttcat cgtcatcgca tcttccgaca agctggtcga
     2341 cgagctacgg ccgcccgtgc cgctcgagct c
//
LOCUS       DASVEU010000189         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_126811,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000189 DASVEU010000000
VERSION     DASVEU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126811"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..645
                     /locus_tag="VF225_07680"
     CDS             334..645
                     /locus_tag="VF225_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145175.1"
                     /translation="MAVTPREGTRLGHEFDEVIDAFRRAERRLRDTLLGDGVYASGTV
                     RGAEARDSVGKDLRQLRTSFDAAAQLVDDAERERLDAALKREAQLAARRAARRAKKPS
                     S"
     gene            complement(642..794)
                     /locus_tag="VF225_07685"
     CDS             complement(642..794)
                     /locus_tag="VF225_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145176.1"
                     /translation="MPVWFLVLLLVILAFAIVLAIGMAYSRRGAGGQNTTIIERDSST
                     TRTTEP"
     gene            948..1376
                     /locus_tag="VF225_07690"
     CDS             948..1376
                     /locus_tag="VF225_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145177.1"
                     /translation="MAGKSDFTEDEWNDLQQGVTGAGLLVSTAHRDFTDSFGEAKAVA
                     THLAAHRESESELVRELAHTRTTGFGLVASPNEVVQGTIDALAASVAVLGQKAPDELE
                     AYRSLVLDVAAGVAEAKGGVKEEESAAIERIREALGSATT"
     gene            complement(1385..1714)
                     /locus_tag="VF225_07695"
     CDS             complement(1385..1714)
                     /locus_tag="VF225_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145178.1"
                     /translation="MTDRNQEKSAFPIAFVGEDGNVVVNFGVLTGREATQAELDRLAH
                     FLRRAGAAPEMTITACRRQDYGPAVEAVVHQVHVEAAGLPVTDFENLCTEWAVDCAED
                     RTVEPLG"
     gene            1785..1982
                     /locus_tag="VF225_07700"
     CDS             1785..1982
                     /locus_tag="VF225_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145179.1"
                     /translation="MIPWLIVALVVVPLVVAAFVVTRRKSAATPELKDPEVEEQFAAA
                     EAFEAEWHEADEERFHKERLP"
BASE COUNT          333 a          678 c          706 g          309 t
ORIGIN      
        1 ccgtgaagtc cggcgcctgg tcgttgatcg tcagggtcat gtgctgctcc tcccgggcgg
       61 gcaccaccca ccccttcgtc gggcgctgtg tacgtgacgg ctgaacgaac gccgcacgtg
      121 cattgttcca agctcgcggc ggcacacgcg gcgctccggc tctcgatctc acccgtgcgg
      181 ggcatgtcca cgcggcgtgg tcggcctacc ctcgtccgag gcccgggcgt ccgggtgccg
      241 tggtgcctag ccgcagggcg cctcaacggc tgaccttgcg cccgggtcga ctccctcgag
      301 cctgcccaca tctgctaaag gtgccctgcc ggagtggccg tcactccgag agagggaacc
      361 cggttgggac acgagttcga cgaggtgatc gacgcctttc ggcgcgccga gcggcgcctc
      421 cgcgacacgc tcctcggcga cggcgtgtac gcgagcggta ccgtacgggg cgccgaggct
      481 cgcgactccg tcggcaagga tctgcgtcag ctgcgcacga gcttcgatgc ggccgcacag
      541 ctggtggacg acgcggagcg cgagcggctc gacgccgcgc tcaagcgcga ggcgcagttg
      601 gcagcgcggc gggcggcgcg ccgcgccaag aagccctctt cctagggctc cgtcgtcctg
      661 gtggtggacg agtcgcgctc gatgatggtg gtgttctgac cgcctgcgcc acggcggctg
      721 tacgccattc cgatcgcaag cacgatcgcg aacgccagga tcacgagaag caggacgaga
      781 aaccagaccg gcatctattc acccccgtag gtaacgacac ggacgcggtg tgatacccgc
      841 cacggccacc ggatgaaacg ccgcttggcg gccggctttc ctgggtagca ctatggcctc
      901 agccgccggc cgagggaacc atcgacgaaa cggataggag cgcacacatg gcaggcaagt
      961 cggacttcac agaggacgag tggaacgacc tccagcaggg cgtcacgggc gcgggtctgc
     1021 tcgtgtcgac cgcacatcgt gacttcacgg acagcttcgg cgaggcgaag gccgtcgcga
     1081 cgcacctcgc cgcgcatcgc gagagcgaga gcgagctcgt ccgcgagctc gcgcacacgc
     1141 ggacaacggg attcgggctc gtcgcctccc cgaacgaggt cgtccagggg acgattgacg
     1201 cgctcgcagc ctcggtcgcc gtgctcggtc agaaggctcc ggacgagctc gaggcctatc
     1261 ggtcgctcgt gctcgacgtc gccgccggcg tcgcggaggc caagggcggg gtcaaggagg
     1321 aggagagcgc ggccatcgag cggatcagag aggcgctcgg ctccgccacg acgtaggcgg
     1381 cgggtcagcc gagtggctcg acggtgcgat cctccgcaca gtcgacggcc cactccgtgc
     1441 acaggttctc gaagtcggtg acgggaagcc cggcggcctc gacgtgcacc tggtggacga
     1501 cggcctcgac tgcaggcccg tagtcctgac ggcggcaagc cgtgatcgtc atctcgggcg
     1561 ccgctccggc gcgtcgcagg aagtgcgcga gccgatccag ttcggcctgc gtcgcctcgc
     1621 gtccagtgag gacgccgaag ttcacgacca cgttcccgtc ctcgccgacg aatgcgatcg
     1681 gaaaggcgct cttctcctga tttcggtcag tcacacgaca caccatttcc ggacgaccgc
     1741 gttgcgtcaa gccacgtgca acgccggcct cgtatgctca gcgtgtgatc ccgtggctca
     1801 tcgtcgctct ggtggtcgtg ccgctcgtcg tcgccgcgtt cgtagtgact cgtcgcaagt
     1861 cggcggcaac gcccgagctg aaggacccgg aggtcgagga gcagttcgcc gctgcggaag
     1921 ccttcgaggc cgaatggcac gaggcagacg aggagcgttt tcacaaggaa cggctgccgt
     1981 agggaagcga gccatgcgtg gggaatagag tttgctccaa cccgag
//
LOCUS       DASVEU010000190        14525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_323378,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000190 DASVEU010000000
VERSION     DASVEU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323378"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VF225_07705"
     CDS             <1..1605
                     /locus_tag="VF225_07705"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7145180.1"
                     /translation="FDGTSMAAPHVSGAAALLLQLHRGWSPQQVKSTLVSTADAAWDD
                     TARTQEAPVTLEGGGLVNLPRAARPLVFTEPASLSFQDLDVNHGRDSRALLVRVTDAG
                     GGAGAWDVQLVPQATSAGGSLDVPPALVVPPGGEADLVAVARGSADAAAGEDYGFILL
                     RRGEITRKIPYEFFVGRPQLGLLQAKRLERFQLGDTVNGPNRVSAYCCPSEPFGPPPD
                     YVGPPMSETGTETLYVTSIKQPVANAGVAIESASAGSSVDPWFLGSPNERDVQGYAGT
                     PVNVNELMSDFSFDIGAAGASFPKVQRFYVSVDSGSDDFTHRSLPGSYVLRSWINDVR
                     PPRVRLLTKRVAAGRPTIVARVTDKGAGVDPLSLVIGYRGVLVGAVLYDPTTGIAVFP
                     LPQQASRIPKGHTRAVITAADYQESKNVNSVGSDILPNTAFRQVGITGVSGPALTWVT
                     PAENACVARTAALVTVASSTKQVRSVRFLVDGKQIAIDRKGTADVFTRTWATRLVPAG
                     RHELRAVATDAGGRTFAATRDVKVCR"
     gene            1596..2369
                     /locus_tag="VF225_07710"
     CDS             1596..2369
                     /locus_tag="VF225_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7145181.1"
                     /translation="MPVDHDRVAVVTGASSGIGEAIARALAGEGWRLVLLARREDRLR
                     ALADELEGEYEVCDVGRREDVERVATSVLARHPQIHLLVNNAGIPGRAGFATIELDRL
                     EEVLRVNYLGAVWSLRAFLPGLEAAGRSDVVNIVSVAGTVAFAPSGPYSASKHAELAF
                     SRSTAAELRGRGIHVHTVNPGFVETEGFPQASALRSALLRRLVVGPEHVARHVLEVLE
                     RDKRETFVPRWYRAAAIAQALAPGLVARLSSRGTYRGGS"
     gene            complement(2366..3088)
                     /locus_tag="VF225_07715"
     CDS             complement(2366..3088)
                     /locus_tag="VF225_07715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7145182.1"
                     /translation="MPESSSNVDDVRARFGATAERVADHARQQIETVREQLRTFVAPG
                     GNERAIDSGTGAGTLALALAPLVREVVGVDIVPELLERARKGAPENVTFVEGDATNLP
                     FETGSFDLACTRRTLHHIAHPEPAIAELARVTVPGGRIFVDDQIAPVDPLDAFALDRF
                     ERARDPSHTRTLPDVDFRQLFEANGLVLIRTRFQTHRRELDYYLDLAGCEGDARERAK
                     QLSPGGPLAYVAESGWYLLRKP"
     gene            complement(3081..4103)
                     /gene="alr"
                     /locus_tag="VF225_07720"
     CDS             complement(3081..4103)
                     /gene="alr"
                     /locus_tag="VF225_07720"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7145183.1"
                     /translation="MHRSEITIDLGAVRRNVRTLLRALDGSRLWAVVKADGYGHGATD
                     VAGAALGAGATALCVATIPEGLALRHEYRTERILVMGPAGSNREVAQAREAGLELAIA
                     DGEIPEGVRVHLKLDTGMGRYGLSELPSPPAEVVGLMTHLATADSDAAFARAQLERFR
                     SATEQYSHLTRHAANSAAALRLPDSRFDAARCGVAIYGLSPFNTDPQEDGLEPVLSWQ
                     SALAQVKRLEPGQSSGYGRRFLADAPAWIGIVPVGYGDGFRRDLTGTEVRVAGELRRV
                     VGTVSMDSFAVELDRELPVGAPVVLLGHGVLAEAHARVADTINYELVCGIDSGPLRAR
                     RTVIDA"
     gene            complement(4103..5422)
                     /locus_tag="VF225_07725"
     CDS             complement(4103..5422)
                     /locus_tag="VF225_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7145184.1"
                     /translation="MFEPLYTADEMRAAEQGHDVDALMQRAGRAVADNVLHRYPDARR
                     IAAVCGQGANGGDGRIALRLLADSGRETSEELEGNPDVVIDALFGTGFRGEPRPDASL
                     LIERINGAGVPVVAVDIPSGVDASTGEVPGAAVRANVTVTMHGRKVGLVLAPGRFHAG
                     EVEVADIGLDHADTDHRLVTDAILRLVPRRSEGDTKYTAGSVLVVGGSPGMTGAVCLA
                     AEAAFRADAGYVAVAVPEESLPVVETLLIEAVKAPRERVDELAKRASAFAVGPGLGRG
                     DEDRALARRLLTDLELPAVVDADALFELEAAEWPAPRVLTPHAGELGRLLDEKSSWVD
                     SHRLAAVRRAVDRYSCVVLLKGAGTLVAAPGEGVLVCDAGTAALATAGTGDVLTGVIA
                     AFLSKGMDARLAAAAGAVAHGRAAELAPHQVGLVASDVIESLQLALS"
     gene            complement(5422..5787)
                     /gene="acpS"
                     /locus_tag="VF225_07730"
     CDS             complement(5422..5787)
                     /gene="acpS"
                     /locus_tag="VF225_07730"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7145185.1"
                     /translation="MTVGVDLIEIERVRRALERYPRFRERCFTDAERAYCDSRPNPAQ
                     SYAGRFAGKEAVGKALGFGVARAFAWRDIEIAGRPKPSVQLSGRIAAWAERMGAGSID
                     LSMTHSRELANAVAVVETH"
     gene            complement(5795..6571)
                     /locus_tag="VF225_07735"
     CDS             complement(5795..6571)
                     /locus_tag="VF225_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX7145186.1"
                     /translation="MAAILLDVDGVFHVSGEPIAGGADAVRRLRADGHRLRFVTNNTT
                     RARATLAEELRAMGIELDDEELQTTPRAAAQTLAGRRVLALTMHAIVGELEGIELVGE
                     DAEAVLIGGADETPETNLVFSYMNLARAFHELEAGAQLYSLHKNRWWQTRNGPLLDAG
                     VFVAGLEYAAETDAIVLGKPSTPYFEAALRALDAEPSLTWMVGDDIEADIGGAVAHGM
                     KSILVRTGKFRPDAVEASRARPDGIISSIAQLPDWLEEHL"
     gene            6570..6983
                     /gene="arfB"
                     /locus_tag="VF225_07740"
     CDS             6570..6983
                     /gene="arfB"
                     /locus_tag="VF225_07740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX7145187.1"
                     /translation="MDGESIRVTRSVVLPLAEVQLRFSRSSGPGGQHAQKSETRVEAI
                     FDVEASNALTDTQKRRVISRAGPVLRALAQDERSQHRNRELAVERLVDQLRRALRVER
                     RRVPTKPTEASRRRRLEDKQRRAKTKRLRREKTDD"
     gene            7034..7423
                     /locus_tag="VF225_07745"
     CDS             7034..7423
                     /locus_tag="VF225_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7145188.1"
                     /translation="MAPISVTLARRDPPAAVVALVGEHDAYSAQRLENELAVLLDEGR
                     RIVVDLRDASFIDSTTLSVLLVGRQRAERSSLGFALLLPDRMHTEVHRILDLTGLGPA
                     FAIFGKLDEALAAVRAGRVAGSPVRAA"
     gene            7462..8256
                     /locus_tag="VF225_07750"
     CDS             7462..8256
                     /locus_tag="VF225_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEX7145189.1"
                     /translation="MDYEPLRRGFAEFVGTFAFVFVGAGSVLTIAKALAPAIVNAQAL
                     DVYGGLTLVGVALANGVAIAVMVSAVGHISGGHLNPAVTLGFLVTRRIAPSLAIVYWS
                     MQFGAAAAAAALLRWLYPETARRITHLGAPGLTSGVSVWQGLVIEIVLTFFLVWVVFA
                     TAADPGGAFKSIAGLAIGLTITVDVLMGGPMTGAAMNPARAFGPELLSRHWSDAWVWY
                     VGPFVGGALAALVYDTLYLRTPRPLLPVGPPDSGVAEPRPGDTAAS"
     gene            8282..9055
                     /locus_tag="VF225_07755"
     CDS             8282..9055
                     /locus_tag="VF225_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145190.1"
                     /translation="MSKPLAVVPELEAELDDLYAGPPESFIRARNDLAQRLKQAGQID
                     GAARVKQLRKPTVPLWAVNQLARRYPDEVRALLEAGDRLRVAQEAALRGETQALRAAT
                     ENERKLLRSLTQLGEELLLETGHSADPKRIVSTLRTAAVDPAGRELLEQGRLSEELEA
                     TGFDAFAGMAVPSTSKPEEKTPKAPSPATQRRRQERLRKLRDNVTKAKKEATSAERAV
                     TRAEASLAEAQQKFTQANHSLRRAQTELEEAEVESARKD"
     gene            complement(9056..10885)
                     /gene="glmS"
                     /locus_tag="VF225_07760"
     CDS             complement(9056..10885)
                     /gene="glmS"
                     /locus_tag="VF225_07760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7145191.1"
                     /translation="MCGIIGYVGSRAAKELLLHGLERLEYRGYDSAGIALRENGSLEY
                     VRAVGNLQFLKDAAGPNGSTSNHGLGHTRWATHGAVNEQNAHPLTGCEDARLAIVLNG
                     IIENYRELKESLQAEGHRFSSETDAEVVVHLIERAYEGDLLEAVRAVYRELEGHFAFV
                     VIHHDHPDLLVGARHQCPLVVGVGEGEMFLASAVAAFLKETRKVQLIEDGEVVAITPE
                     GARFFTDEGEVEHKEVEPIDWDDESAVKGGYETFMLKEIYEQPEAVRETIGDRARRGK
                     LVLEGIGLNELEIQNLRRIVIVACGTAYHAGVVGRYIIEEWARVPVEPDIASEWRYRN
                     PVLSKDTLVIGITQSGETADTIAAMRLAREQGARTLAITNQLGTQITREVDAVLYTRA
                     GLEVGVAATKTFTAQVALLSLIALKLAQIRRTLPAKEIEFILEELFDLPEKMEQFLDA
                     DHPIEEIAQRHYNKPFFLYLGRHIGLPVALEGALKLKEISYIPTEAYSAGEMKHGPIA
                     LLDSDTPVVCIAIDQKVVYEKVVSNIQEVRARGAQVIAIATDGNEDIQHHADDVIYIP
                     RTPAFLQAVLAVLPLQLLAYRIARLRGLNVDQPRNLAKTVTVE"
     gene            complement(11016..12281)
                     /locus_tag="VF225_07765"
     CDS             complement(11016..12281)
                     /locus_tag="VF225_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7145192.1"
                     /translation="MPRRYFGTDGVRGIVGEDLTPDLVERLGRAATLWSGRGRIFVGR
                     DTRASGPELEGALARGIASVGGNAVVAGVLPTPAVALLTLDLGCVITASHNPPEYNGV
                     KFFDRDGQKLSDTQEEEIEALLDTPATGGGDIDEVGVATDSYLEHVVERFGSDLSGLR
                     IGVDCANGAYSGLAPKAFTELGAVVTAIGDEPDGTNINVGCGATDLRALQRTVTELGL
                     DLGVAFDGDGDRMLAVDGNGDVVDGDQIVAVLALDREVDLVAVTVMANMGLHRLMAEN
                     GVRVVTTDVGDRYVLEALRREGGILGGEQSGHLIWLDGHVTGDGLVAALLLCNALHGR
                     SLAEVAAVMPRFPQAKENVSFERHGRLSPAIEEEVARLNAELGERGRVLVRPSGTEPV
                     IRVLAEAETGQEAQTLCGTIATLVRRELG"
     gene            complement(12413..12808)
                     /gene="rpsI"
                     /locus_tag="VF225_07770"
     CDS             complement(12413..12808)
                     /gene="rpsI"
                     /locus_tag="VF225_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7145193.1"
                     /translation="MSQIAQYRGTGKRKTSVARVILRPGDGNTWFNGRTIEDYFPRAA
                     HRTSALAPLQIAGVEGTFDIRVRVHGGGPSGQAGAVRHGIARALVEADPELRVPLKRA
                     GFLTRDARIVERKKAGLHKARKAPQFSKR"
     gene            complement(12805..13242)
                     /gene="rplM"
                     /locus_tag="VF225_07775"
     CDS             complement(12805..13242)
                     /gene="rplM"
                     /locus_tag="VF225_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7145194.1"
                     /translation="MKTYNAKPGEIARHWYVVDADGQTLGRLATRIADQLRGKGKPEY
                     TPHVDTGDFVIVVNAEKIQVTGDKLDQKRYYRHSGYPGGIRSRTLREQLERRPEEVLR
                     AAVKGMLPKNRLARQQITKLKIYAGPEHPHGPQAPQPLKWEDR"
     gene            13371..>14525
                     /locus_tag="VF225_07780"
     CDS             13371..>14525
                     /locus_tag="VF225_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145195.1"
                     /translation="MRGRTAALVAACAGVCVPAAAAAPPQGSQTDAAFAASYAQRGVS
                     SVAATTQRVSCYTPEVYYQGSLTPAQGYPDGGSTLCNGAATTGENIGPYPTQDVSNPP
                     LRAKDFSESDLHVDPTNARHLIGISKWIVNSEGYNHLTGFFESFDGGATWPQQGHLPG
                     YEGWTDNSDPVGAFDPWGNFYAVVFPYQFSYIASGQHFFLSPDVNPSLPRSGLGIAVR
                     PHGASAATTWNTTHGSQLDLIEQTPFEGKSTFDKQWLAIDTNRRSPHFGRVYVTWAVG
                     NQDSDLRIYGSYADAHKNGTHTNWSHPRLVLPQARGIGDNGSLPRVALDGTVWLATSS
                     FGNSNGPYTMSLTSSRNGGKSWARRRVIVRHTVNGYRNTTFRAAFGEAFAV"
BASE COUNT         2260 a         5067 c         4854 g         2344 t
ORIGIN      
        1 ttcgacggca cgagcatggc cgcaccacac gtctcgggcg ctgcggcgct gctccttcag
       61 cttcaccgcg gctggtcacc gcagcaggtc aagtctacgc tcgtctcgac cgcggacgca
      121 gcctgggacg acactgcgcg cacccaggag gccccggtca cgctcgaggg cggaggcctc
      181 gtcaacttgc cgcgtgcggc gcggcctctc gtgttcacgg agccggcgtc gctctcgttc
      241 caggatctcg acgtcaacca cggcagggac agcagggcgc tcctcgttcg cgtcactgat
      301 gccggcggcg gcgccggggc ctgggacgtg cagctcgtcc cgcaggcgac ctcggccggt
      361 ggctcgctcg acgtgccgcc tgcgctcgta gtcccgcccg gcggcgaagc ggacctcgtg
      421 gcggtggcgc gcggcagcgc agacgcggcg gcaggcgagg actacggttt catcctccta
      481 cgtcgcggcg agatcacccg caagattccc tacgaattct tcgtcggcag gccgcaactc
      541 ggactcctgc aggcgaagcg cctcgaacgc ttccaactcg gagacacggt gaacggaccg
      601 aaccgcgtct ccgcgtactg ctgtccgtcc gagccgttcg gtcctccacc ggactacgtc
      661 ggcccgccaa tgagcgagac cggtacggag acgctctatg tcacgagcat caagcagccc
      721 gtcgccaacg ccggagtcgc gatcgagtcg gcgagcgcgg ggtcgagcgt cgacccgtgg
      781 ttcctcggct cccccaacga gcgcgacgtg cagggatacg ctggaacccc ggtcaacgtc
      841 aacgagttga tgtcggactt cagcttcgac ataggggcag ccggcgcgtc gttcccgaag
      901 gttcagcggt tctatgtctc ggtcgactcg ggctcggacg acttcacgca ccgttcgctt
      961 cccggcagtt acgtgcttcg ctcgtggatc aacgacgtgc gcccgccgcg tgtccgtctg
     1021 ctgacgaagc gtgttgccgc cggacgtccg accatcgtcg cgcgtgtgac agacaagggc
     1081 gccggcgtcg acccgctgtc gctcgtgatc ggttaccgag gcgtactcgt cggcgcggtg
     1141 ttgtacgacc cgaccaccgg aattgccgtc ttcccattgc cgcagcaggc ctccaggatc
     1201 ccgaaagggc acacgcgcgc ggtgatcacc gccgccgact atcaggagtc gaagaacgtc
     1261 aattcggtcg gctccgacat cctgcccaac acggcgttca gacaggtggg aatcaccggc
     1321 gtctccggac cggcgctcac gtgggtcacc ccggccgaga acgcgtgcgt cgcgaggacg
     1381 gccgccctgg tgactgtcgc gagctcgacg aagcaagtgc ggtctgtgcg cttcctcgtc
     1441 gacggaaagc agatcgcgat cgaccggaag ggcacggccg acgtctttac tcgcacctgg
     1501 gccacgcgac tcgttccggc tggaaggcac gagctgcgag ccgtcgcgac ggacgccggc
     1561 ggccgcacct tcgccgcgac gcgcgacgtg aaggtctgcc ggtagatcac gaccgcgtag
     1621 ccgtcgttac cggtgcctcg agtgggatcg gcgaggcgat cgcccgcgcg ctcgcaggcg
     1681 aaggctggcg cctcgtgctg ctcgcccgcc gggaggaccg cctgcgcgcg ctcgccgacg
     1741 aactcgaagg cgagtacgag gtgtgtgacg tcggtcgccg cgaagacgtc gagcgggtgg
     1801 caacgtcggt actggcgcgt catccgcaga ttcacctgct cgtgaacaac gctggtatcc
     1861 cgggacgcgc ggggtttgcc acgatcgagc tcgaccggct cgaggaggtc ctgcgcgtga
     1921 actacctcgg tgcggtgtgg tcattgcgcg cgttcctgcc ggggctcgaa gctgcgggac
     1981 gctccgacgt cgtcaacatc gtctcggtcg caggcacggt cgcgttcgcc ccgtctggcc
     2041 cctactccgc ctccaagcat gcggagctcg ccttctcccg ctccaccgcc gcggagctgc
     2101 gcggtcgagg tatccacgtt cacaccgtca acccggggtt cgtcgagacg gagggattcc
     2161 cgcaggcgtc ggcgctgcgc agcgcgctcc tgcggcgact cgtcgtcggc ccggagcacg
     2221 tggctcgaca cgtcctcgag gtcctcgagc gcgacaagcg agagacattc gtgccgcggt
     2281 ggtaccgggc tgccgcaatc gcgcaggcac tcgcgccggg cctggttgcg cgactctcct
     2341 cccgcggtac ctaccgcggt ggctcctagg gcttgcgcag gaggtaccag ccgctctcgg
     2401 ccacgtaggc caggggcccg ccgggagaga gttgctttgc ccgttcccgt gcgtcgccct
     2461 cgcagccggc gagatcgagg tagtagtcga gctcgcgccg atgcgtctgg aagcgcgtcc
     2521 ggatcaggac gaggccgttg gcctcgaaga gctgccggaa gtcgacgtct ggcagcgttc
     2581 gcgtgtgcga gggatcgcgt gcgcgctcga accggtcgag ggcgaacgcg tcgagcggat
     2641 ccacgggcgc gatctggtcg tcgacgaaga tgcgcccgcc gggcaccgtg acgcgcgcca
     2701 gttctgcgat ggccggctca gggtgcgcaa tgtgatgcag cgtgcgccgc gtgcaagcga
     2761 gatcaaacga gccagtctcg aacggaaggt tcgtcgcgtc gccctcgacg aacgtgacgt
     2821 tctccggcgc gcctttgcgg gcccgctcga gcagctcggg gacgatgtcg acgccgacga
     2881 cctcgcggac gagcggcgcc agtgcgagcg cgagcgtgcc cgcaccggtc ccgctgtcga
     2941 tcgcccgctc atttccaccc ggcgcgacga aggtgcgcag ctgctcgcgt accgtctcga
     3001 tctgctgccg agcgtgatcc gccacgcgct cggccgtcgc cccgaagcgg gcgcgcacgt
     3061 catcgacgtt cgagctcgac tcaggcatcg atcaccgtgc ggcgcgcgcg tagaggtccg
     3121 ctgtcgattc cgcacacgag ctcatagttg atcgtgtccg cgacgcgggc gtgcgcctcg
     3181 gcgagcacgc cgtgcccgag taggacgacc ggcgcgccga ccggcagctc cctgtcgagc
     3241 tcgaccgcga atgaatccat cgacaccgtc ccgaccacgc gccggagctc gcctgcgacg
     3301 cgcacctcgg tacccgtcag gtcgcgccgg aagccgtccc cgtagccgac ggggacgatc
     3361 ccgatccacg cgggtgcgtc ggcgaggaaa cgccggccgt aaccggagct ctgcccgggc
     3421 tcgagccgct tcacctgtgc gagcgcagac tgccaggaaa ggaccggctc gagcccgtcc
     3481 tcctgtggat ccgtgttgaa cggcgaaagc ccgtagatcg cgaccccgca gcgggccgcg
     3541 tcgaagcgtg agtccggtag ccgtagcgcg gctgcactgt tcgcagcgtg tcgcgtgagg
     3601 tggctgtact gctccgtcgc cgaccggaac cgttcgagct gggcgcgcgc aaacgcggca
     3661 tcgctgtccg ccgtcgcgag atgagtcatc aacccgacga cctccgcggg cggcgacggg
     3721 agctcggaga ggccgtaacg gcccatgccg gtatcgagct tcagatgcac acgcacgccc
     3781 tcgggtatct caccgtcggc gatcgcgagc tcgaggccgg cttcccgggc ctgcgcgacc
     3841 tcccggttcg agccggcggg gcccatgacg aggatccgct cggtgcggta ttcgtggcgc
     3901 aaggccagtc cttcggggat ggtcgcgacg cagagcgccg tcgcgccggc gccgagtgcc
     3961 gcgcctgcaa catcggtcgc gccatggccg tagccgtcgg ccttgacgac cgcccacagc
     4021 cgtgagccgt cgagggcgcg caagagcgtg cggacgttgc gtcgaaccgc gccgaggtcg
     4081 atggtgatct ccgagcggtg catcagctga gtgcgagttg caacgactcg atcacgtcgg
     4141 acgcgacgag gccgacctgg tgcggtgcga gctcggcggc tcggccgtgt gccacagcac
     4201 ccgcggcggc ggcgagacgc gcgtccattc ccttcgacag gaatgctgcg atcacaccgg
     4261 tgagcacgtc accggttccg gccgtcgcca gcgcggccgt cccggcgtcg cacaccagca
     4321 cgccttcccc gggtgcggcc acgagcgtgc ccgctccctt cagcaggacg acgcagctgt
     4381 agcgatcgac tgcgcgccgc acagctgcga gccgatgcga gtcgacccag ctcgatttct
     4441 catcaagcaa acggccgagc tcgcctgcat gaggagtcag cacccgtggt gcgggccatt
     4501 cggccgcctc gagctcgaag agcgcgtccg cgtcgacgac tgccggcagc tccaggtctg
     4561 tcagcagccg gcgcgcgagc gcgcggtcct cgtcgccgcg accgagcccg gggccgaccg
     4621 caaaagcgga tgcgcgcttc gcgagctcgt ccacacgttc gcgcggcgcc ttgaccgcct
     4681 cgatgagcaa tgtctcgacg accggcaacg actcctcggg caccgcgacc gccacgtacc
     4741 ccgcatcggc gcggaacgcc gcttcggcag ccaggcagac ggcgcccgtc atcccaggcg
     4801 acccgccgac gacgaggacg gatcccgcgg tgtacttggt gtcgccctcg ctcctacgcg
     4861 gcaccagccg gaggatcgca tcggtcacga ggcggtggtc cgtatccgcg tggtcgagcc
     4921 cgatgtcggc aacctcgacc tccccggcgt ggaagcgacc cggcgcgagg acgagcccga
     4981 ccttgcggcc gtgcatggtc acggtcacgt tggcgcgcac ggcggcgccg ggaacctctc
     5041 cggtcgacgc gtcgacgccc gaagggatgt cgactgcgac gaccggaacg ccggcgccgt
     5101 tgatgcgttc gatcagcagg gacgcgtccg gacggggctc gccgcggaag ccggtcccga
     5161 agagagcgtc gatgacgacg tcggggttgc cctcgagctc ttcgctcgtc tcacgccccg
     5221 aatcggcaag cagccgtagt gcgatccgtc cgtcgccgcc gttcgctccc tggccgcaaa
     5281 ccgccgcgat ccggcgggcg tccggatagc ggtgcaggac gttgtccgcg accgcgcggc
     5341 ccgcccgttg catcaatgcg tcgacatcgt gaccctgctc ggcggcgcgc atctcgtccg
     5401 ccgtgtagag cggctcgaac atcagtgggt ctcgacgacc gccaccgcgt tcgccagctc
     5461 gcgagagtgc gtcatcgaca ggtcgatcga cccggctccc atccgctcgg cccaggcagc
     5521 gatccggccg ctgagctgca cggacggctt cggccgcccc gcgatctcga tgtcacgcca
     5581 cgcgaacgcg cgagcaaccc cgaacccgag tgctttcccg accgcctctt tgccggcgaa
     5641 cctgcccgcg tagctctggg caggattcgg ccgcgagtcg cagtacgcgc gctcggcgtc
     5701 ggtgaagcag cgctcgcgga agcggggata gcgctcgagc gcgcggcgca cgcgctcgat
     5761 ctcgatcagg tcgacaccga cggtcatagg cagctcacag atgttcctcg agccaatcag
     5821 gtagctgcgc gatggacgag atgatcccgt ccggccgggc acggctcgcc tcgaccgcgt
     5881 cgggcctgaa cttccccgtg cggacgagga tggacttcat gccatgcgcc accgcgccgc
     5941 cgatgtcggc ctcgatgtcg tcgccgacca tccacgtgag actcggctcc gcgtcgagcg
     6001 ccctcaacgc ggcttcgaag tagggagtcg aaggcttgcc aaggacgatc gcatccgttt
     6061 ccgcggcgta ctcgagcccg gcgacgaaaa cgccggcgtc gaggagcggc ccgttcctcg
     6121 tctgccacca ccggttcttg tgcagcgagt agagctgcgc gccggcctcc aactcgtgga
     6181 aggcgcgcgc caggttcatg tagctgaaga cgagatttgt ctctggggtc tcgtcggcgc
     6241 cgccgatgag aaccgcctcc gcgtcctcgc cgacgagctc gatcccctcg agctcgccca
     6301 cgatcgcgtg catggtgagc gcgagaaccc ggcgcccggc cagtgtctgc gccgccgccc
     6361 gcggcgtggt ctgcagctct tcgtcgtcga gctcgattcc catagcgcgc agctcctcgg
     6421 ccagggtcgc ccgcgcgcgc gtcgtgttgt tggtgacgaa gcggagcctg tgcccgtcgg
     6481 cacgaagtcg ccgcacggcg tcggccccgc cggcgatcgg ttcgcccgag acgtggaaga
     6541 caccgtcgac gtccagcaga attgcggcca tggacggcga gtctattcgc gtgacccgtt
     6601 ccgtcgttct gccgctcgcg gaggtgcagc tccgcttctc gcggtcgagc gggcccggcg
     6661 gacagcacgc ccagaagtcc gaaacgcggg tcgaagcgat cttcgacgtg gaggcgtcga
     6721 acgcgctcac ggacacccag aagcgccgtg tcatctcccg ggcaggaccc gttctgcgcg
     6781 cgctcgcaca ggacgagcgc agccagcacc ggaatcgcga gctcgccgtc gagcggctcg
     6841 tcgatcagct ccgccgggcg ctccgggtcg aacgccgccg ggtgccgacg aagccgaccg
     6901 aggcctcccg gcggcgccgg ctcgaggaca agcaacgaag agccaagacg aagaggctga
     6961 ggcgagagaa gacggacgac tgacgccacc cgtacccatc cgggccgggc ccgcgtttta
     7021 ctcctcgaaa agaatggcgc cgatctccgt caccctagct aggcgggatc caccggctgc
     7081 cgtggtcgcc ttagtcgggg agcacgacgc ctactccgct cagcggctcg agaacgagct
     7141 cgccgtcctg ctggacgagg gccgccggat cgtcgtcgac ctgcgcgacg cctcgttcat
     7201 cgactcgacg acgttgagcg tcctcctcgt tgggcgccag cgcgctgaac gatcgtcgct
     7261 cgggtttgcg ctactgctgc ccgatcggat gcacacggag gtccatcgga tcctggatct
     7321 gaccggcctc ggcccggcct tcgcgatctt cgggaagctc gacgaagcgc tcgcggccgt
     7381 tcgggcgggc cgggtcgccg ggagcccggt tcgagcggcg taacacgtac cgtccgaccc
     7441 tccccgtagc ttccatccgc gatggattac gagccgctcc ggcgtggctt cgccgagttc
     7501 gtcggcacct ttgcgttcgt tttcgtcggc gccgggtcgg tcctcaccat cgccaaggca
     7561 ctggcgcccg cgatcgtcaa cgcgcaggcg ctcgacgtct acggcggtct gaccctcgtc
     7621 ggcgtcgccc ttgccaatgg ggtcgcgatc gccgtcatgg tctccgcggt cggacatatc
     7681 tcgggtggtc atctcaaccc tgccgtgacg ctcggattcc tcgtcacccg ccgcatcgcg
     7741 ccgtcgctgg cgatcgtcta ctggagcatg cagttcggcg cagcagcggc cgcagccgcc
     7801 ttgctccgct ggttgtatcc cgagaccgcg cgtcgaatca cgcatctcgg ggccccgggc
     7861 ctgacgagcg gggtcagcgt ctggcaaggg ctcgtgatcg agatcgtgct gacgttcttc
     7921 ctcgtctggg tggtcttcgc caccgccgcc gatccgggcg gcgccttcaa gtcgatagcc
     7981 ggtctggcga tcgggctgac gatcacggtg gacgtcttga tgggcggccc gatgacgggc
     8041 gccgcgatga atccggcccg cgccttcggg cctgagctcc tgtcacgcca ctggagcgat
     8101 gcctgggtct ggtacgtcgg cccgttcgtc ggcggcgcac tggccgcgct cgtctacgac
     8161 acgctctatc tccggacgcc gaggccgctg ttgccggtcg gcccgcccga ttcgggcgtg
     8221 gccgagccgc ggcccggcga caccgccgcc tcctagagcg caggattgct acaacgcggc
     8281 ggtgtcgaag ccgctcgccg ttgtgcccga gctcgaggcc gagctcgacg acctgtacgc
     8341 tgggccgccc gagagcttca tccgggcgcg taatgacctc gcgcagcggc tcaagcaggc
     8401 aggccagatc gacggcgccg cgcgggtcaa acaactgcgt aagcccacgg tccccctgtg
     8461 ggccgtaaac cagcttgcgc ggcgctatcc cgatgaggtt cgcgcgctgc tcgaagcagg
     8521 cgaccggctg cgagtcgcgc aggaggccgc cttgcgcggt gagacacagg cacttcgcgc
     8581 ggcaactgag aatgaacgca aacttctgcg cagcctgacg cagctaggcg aagagctgct
     8641 gctcgagacc ggacattcgg cagacccgaa acgcatcgtg agcacgcttc gcacagccgc
     8701 ggtcgatccg gccggccgcg agctgctcga gcagggccga ctgagcgagg agctcgaagc
     8761 gaccggattc gacgccttcg ccggcatggc tgtcccttcc acatcgaagc cggaggagaa
     8821 gacgccgaag gcaccctcgc ctgcaacgca acggcggcga caggagagac tccgcaagct
     8881 gcgtgacaac gtcacgaagg ccaagaagga ggcgacgagc gcagagcgcg ccgtgacccg
     8941 agcggaggcg agcctggccg aggcccagca gaagttcact caagcgaatc actccctgcg
     9001 acgcgcacag accgagttgg aagaggccga ggtggaatcg gccagaaagg attagctact
     9061 cgacggtaac ggtcttcgcg aggttccgcg gctggtcgac gttcagtcca cgcaatcgcg
     9121 cgatcctgta ggcgagcagc tggagcggga gcaccgcgag caccgcctgc aggaacgcag
     9181 gcgtccgcgg gatgtagatg acgtcgtcgg catgatgctg gatgtcctcg ttcccgtcgg
     9241 tcgcgatcgc gatcacctgg gcgccgcgcg cccttacctc ctgaatgttc gagacgacct
     9301 tctcgtagac caccttctga tcgatcgcga tgcacaccac cggagtgtcg gagtcgagga
     9361 gggcgatcgg gccgtgcttc atctcgccgg ccgagtacgc ctcggtcggg atgtaggaga
     9421 tctccttcag cttgagcgca ccctcgaggg cgaccggcag gccgatgtgg cggccgaggt
     9481 agagaaagaa cggcttgttg tagtgccgtt gcgcgatctc ctcgatcgga tggtcggcgt
     9541 cgaggaactg ctccatcttc tcgggcaggt cgaagagctc ctcgaggatg aactcgattt
     9601 ctttggcggg cagggtgcgc cggatctgcg cgagcttgag cgcgatcagc gacagcagcg
     9661 cgacctgggc cgtgaaagtc ttcgtcgccg caacgccgac ctcgagtccg gcgcgcgtgt
     9721 agaggaccgc gtcgacctca cgcgtgatct gcgttccgag ctgattcgtg atcgcaagcg
     9781 tccgcgcgcc ctgctcgcga gcgaggcgca tggccgcgat cgtgtccgct gtctcgccgg
     9841 actgcgtgat cccgatcacc agcgtgtcct tcgacaacac ggggttgcgg tagcgccact
     9901 cgctcgcgat gtccggctcg acgggaacgc gtgcccactc ctcgatgatg taccgcccga
     9961 cgacgccggc gtggtacgcc gtgccgcacg cgacgatcac gatccggcgc aagttctgga
    10021 tctccagctc gttgagcccg atcccttcca acaccagctt gccgcgacgc gcgcggtcgc
    10081 cgatcgtctc gcgcaccgcc tccggctgct cgtagatctc cttgagcatg aacgtctcgt
    10141 agccgccctt gactgcgctc tcgtcgtccc agtcgatggg ctcgacctcc ttgtgctcga
    10201 cctcaccctc atccgtgaag aagcgcgcac cctcgggggt gatcgcaacg acctcgccgt
    10261 cctcgatcag ctgcaccttg cgcgtctcct tgaggaacgc tgcaaccgcg gatgccagga
    10321 acatctcgcc ttccccgacg ccgacgacga gtgggcattg atgacgtgcg cccaccagca
    10381 gatcgggatg gtcgtgatgg atcacgacga aggcgaagtg cccttcgagt tcgcggtaga
    10441 cggcccgcac agcctccagg agatcgcctt cgtaggcgcg ctcgatcagg tgcacaacga
    10501 cttccgcatc cgtctcggag ctgaatcgat ggccctcggc ctggagtgat tccttgagct
    10561 cccgatagtt ctcgatgatt ccgttcagca cgatcgcgag ccgagcgtcc tcacaacccg
    10621 tcagcgggtg cgcgttctgc tcgttgacgg cgccgtgcgt cgcccagcgt gtgtgaccga
    10681 ggccgtgatt cgacgtggat ccgttcggtc ccgccgcatc tttcagaaac tggaggttgc
    10741 cgaccgcgcg tacgtactcg agactgccgt tctcgcgcag cgcgatgccg gccgagtcgt
    10801 agccgcggta ctcgaggcgc tcgaggccgt ggagcaacag ctccttcgcg gcccggctac
    10861 cgacgtaccc gatgatcccg cacacccgaa actcctctcc gtcgccgccc cgtgccctca
    10921 gaggtggatc gaaatctgca gattcggttt ttccgaaaac gggctctcga aaacggcaaa
    10981 agcccggcta gcgatccgcc ccttcgccga gacggtcagc cgagctctcg tcgcacgaga
    11041 gttgcgatcg taccacagag ggtttgggct tcctgcccgg tttccgcctc tgccagcacc
    11101 cggatgacgg gctccgtgcc cgacggtcgg acgagcaccc ggcctcgctc tccgagctcg
    11161 gcgttgagcc gcgcgacctc ctcctcgatc gcgggactga gccgcccgtg tcgctcgaag
    11221 gagacattct ccttcgcctg cgggaagcgc ggcatgacgg cggcgacctc tgcgagtgaa
    11281 cggccgtgca atgcgttgca aagcaggagc gcggcgacca gaccatctcc ggtcacgtgt
    11341 ccgtccagcc agatcagatg cccggattgc tcgccgccga gaatgcctcc ttcgcggcgc
    11401 agcgcctcga gcacgtagcg gtcgccgacg tcggtggtga ctacgcggac tccgttctcg
    11461 gccatcaggc gatgcaggcc catgttcgcc atcacggtca ccgcgacgag gtcgacctcg
    11521 cggtcgaggg caagcaccgc gacgatctga tcaccgtcga cgacgtcgcc gttcccgtcg
    11581 acggcgagca tgcggtcgcc gtcaccgtcg aaggcgacac cgaggtcgag cccgagctcc
    11641 gtgacggtgc gttggagcgc gcgcagatcg gtcgctccgc agccgacgtt gatgttcgtg
    11701 ccgtccggct cgtccccgat tgccgtgact actgcgccga gctcggtaaa agccttcggg
    11761 gcgagcccgg agtaggcgcc gttcgcacag tcgacgccga tcctcaagcc gctgagatcg
    11821 gagccgaatc gttcgacgac gtgctcgagg taactgtcag tcgcgacacc gacctcgtcg
    11881 atgtcgcccc cgcccgtggc cggcgtgtcg agtagcgctt cgatctcctc ctcctgcgtg
    11941 tcggacaact tctgtccgtc acggtcgaag aacttcacgc cgttgtattc gggcggattg
    12001 tgcgatgcgg tgatgacgca accgagatca agcgtcaaga gggcgacagc cggcgtcgga
    12061 aggacacccg caacgaccgc gttgccgccg accgacgcga tcccgcgcgc cagcgcgccc
    12121 tcgagctcgg ggccggatgc gcgcgtgtcg cgtccgacga agatgcgccc gcggccggac
    12181 caaagtgttg cggcacggcc cagccgttcg acgagatcgg gggtcaggtc ttcgccgacg
    12241 atcccgcgca ccccgtcggt tccaaagtag cgccgtggca cggcggccga ggatacgtga
    12301 ggaaggggtt tgggcatcat cttcatagga ggacgcggaa gtcgcgtcgt ccgcggccgt
    12361 tcgaccgcgc gaagcgcggg tctagcccgc acaaccgcta aaagcggagc ttttagcgct
    12421 tactgaactg cggtgccttg cgcgccttgt gcaggcccgc cttcttgcgc tcgacgatac
    12481 gggcgtcgcg cgtgaggaag ccggcccgct tgagcgggac acgcaactcc ggatcggcct
    12541 cgacgagcgc gcgcgcgatg ccgtgacgaa cagcgcccgc ctgtccgctc gggccgccac
    12601 cgtggacgcg gacccggatg tcgaacgttc cttccactcc tgcgatctgc agcggcgcca
    12661 gcgcggacgt gcgatgcgcg gcgcgcggaa agtagtcctc gatcgtgcga ccgttgaacc
    12721 acgtgttccc gtcgccgggt cgcaggatca cgcgggcgac ggaggtcttc cgcttgcccg
    12781 tcccacggta ttgcgcgatc tggctcatct gtcctcccac ttcaacggct gcggcgcctg
    12841 cggcccgtgc ggatgctcgg gacccgcgta gatcttgagc ttcgtgatct gctgccgagc
    12901 cagccggttc ttcgggagca tgcccttcac cgcggcgcgc aggacctctt ccggccggcg
    12961 ctctagctgc tcgcgcagcg tccggctgcg gattcctccg ggatagccgg agtgacggta
    13021 gtagcgcttc tggtcgagct tgtcgccggt cacctggatc ttctcggcat tgacgacgat
    13081 cacgaaatcg ccggtgtcca cgtgcggcgt gtattcgggc ttccctttgc cgcgcagctg
    13141 gtccgcgatg cgcgtggcca gccggccgag cgtctgcccg tcggcatcga ccacgtacca
    13201 gtggcgcgcg atctctcctg gtttggcgtt gtaagtcttc atggaaaagg cggactaacg
    13261 gtgtccgccg gcgcaaaact ctagcagtcg gcagcggaat ccggccgccc gtgacctgga
    13321 cgagctcgct tcgaggatcg gggccgcggc gcgtcattat gctcatgcgg gtgagaggac
    13381 gcaccgccgc gctggttgcg gcctgcgcgg gagtctgcgt gcccgctgcg gccgcggcac
    13441 cgccccaggg cagccagacg gatgccgcct tcgcggccag ctacgcccag cgcggggtca
    13501 gcagcgtggc cgcgaccact caacgcgtca gctgctacac gcccgaggtc tactaccagg
    13561 gcagccttac gcccgcgcag ggatatccgg acggcggctc cacgctgtgc aacggcgctg
    13621 cgacgacggg agagaacatc ggcccgtacc cgacccagga cgtctctaac ccgccgctcc
    13681 gtgcgaagga cttctccgaa tccgatctgc acgtcgatcc gacgaatgcg cgccacctga
    13741 tcggtatcag caagtggatc gtgaacagcg agggctacaa ccacctcacc ggcttcttcg
    13801 agtcgttcga cggcggcgcg acgtggcccc agcaaggtca ccttcccggc tacgagggct
    13861 ggacggacaa ctcggacccc gtcggggcgt tcgacccgtg ggggaacttc tacgccgtcg
    13921 tgttcccgta ccagttctcg tacatcgcga gcggccagca cttcttcctg tccccagacg
    13981 tcaatccctc gctgccacgc tctgggctcg ggatcgccgt tcgtccccac ggtgcctccg
    14041 cggcgacgac ctggaacacg acgcacggca gccagctgga cctgatcgag cagactccgt
    14101 tcgaagggaa gtcgaccttc gacaagcaat ggctggcgat cgacacgaat cgccgcagcc
    14161 ctcacttcgg tcgcgtctac gtcacgtggg ccgtcgggaa ccaggacagc gacctgcgca
    14221 tctacggctc gtacgcagat gcgcacaaga acgggacgca cacgaactgg tcgcatccgc
    14281 ggctcgtgct gccgcaggcc agggggatcg gggacaacgg ctcgctgcca cgcgtcgcgc
    14341 tagacgggac cgtctggctg gcgacgtcga gcttcggcaa ctcgaacggc ccgtacacga
    14401 tgtcgttgac ctcgtcgcgg aacgggggca agtcctgggc gcgccgtcgg gtgatcgtcc
    14461 gccataccgt caacggctac cggaacacga cgttccgcgc cgctttcggg gaggcgttcg
    14521 cggtc
//
LOCUS       DASVEU010000191        11580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_713851,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000191 DASVEU010000000
VERSION     DASVEU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11580
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713851"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VF225_07785"
     CDS             <1..934
                     /locus_tag="VF225_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7145196.1"
                     /translation="FHGAEDLRNGVLRTVRSRSFAEDPLRLVRGLRLVSQLGLEPEAE
                     TLQQMRAEASSVALVSGERVGGGLHSDGLGELSKLLLGREPARALRLARDTGVLTALL
                     PEFEPSIGFEQDSERQHLPLDEHTFAVVQAAADADMPLAVRLGALFHDLGKPRANGKH
                     AERGAELAAAAMRRLRYPTKLRTYVTRLVRAHAFSLEDVDELFARRFLREHGDELAFD
                     LVLHKEADLRGKNVSQDELAAAARLRELLEQERSQPHHLTDLAVDGSDLIELGFAEGP
                     VLGRTLESLLDAVVDEPSLNTRDQLLERARMQLS"
     gene            931..1635
                     /locus_tag="VF225_07790"
     CDS             931..1635
                     /locus_tag="VF225_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX7145197.1"
                     /translation="MTVPLIRWDAPGPYAVAFSTRLGGVSVGAYESLNLGRSTRDDSA
                     NVDENRRRLSASVGVEPERLALNRQVHGATVNRAEKGERTQNGDGLWTDEPGVPMLAF
                     SADCLPIALARVNGNPGLALLHAGRLGLLEGILEAGVGALRGPIAAIVGPGIGPCCYE
                     VGVDILAVYRARFGPKVIHGRNLDLWSAAEHVLREAGVEAVERLDVCTACDDRFFSHR
                     RDGPITGRQGVIGYVT"
     gene            1625..2218
                     /locus_tag="VF225_07795"
     CDS             1625..2218
                     /locus_tag="VF225_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HEX7145198.1"
                     /translation="MSPETIRANYERIRSEVGESVTVVAATKYVALEDMRLLAEAGVE
                     VVGENRAQELERKHAVYGDTFRWHFIGHLQSNKVKVVNRSCELVHSLDSDSTARRLAV
                     PALVEVNLAGESSKSGVAPEDVPRLLDLHGDVRGLMTMPPETRDPEASRPYFIRLRKL
                     AEEHGLKELSMGTSQDYRVAAEEGATFVRVGSILYGE"
     gene            2232..2765
                     /gene="sepF"
                     /locus_tag="VF225_07800"
     CDS             2232..2765
                     /gene="sepF"
                     /locus_tag="VF225_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX7145199.1"
                     /translation="MGFADVWNKTLVYFGIAEEEDWDEEAYVTDEDIQRSYADRPNVR
                     RLAPRRREREFDDWTDPEPAPGNDRTTVLRPRVAAVPSPASVRVHLVVPRSFNDAQQI
                     ADKFKESIPVILNLQSSDQDLSKRLIDFASGLTYALDGGMQRVADKVFLLTPRNVEVS
                     AEERARLLERGGFFNQA"
     gene            2802..3113
                     /locus_tag="VF225_07805"
     CDS             2802..3113
                     /locus_tag="VF225_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7145200.1"
                     /translation="MAAGAMLGLLYDAIDNVVGFVQVFITVYSLLILAYIITSWVRLP
                     YSTWLNRIQRFLYDVCDPYLRLWRRILPTFGPLDLSPVVGVAFLYILSRVIVSILNSF
                     H"
     gene            3126..3569
                     /locus_tag="VF225_07810"
     CDS             3126..3569
                     /locus_tag="VF225_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX7145201.1"
                     /translation="MALTPVEIRHLKAARSLVGGYKRTAVDALMDEIVASFEDVWRER
                     ADLADKLEQLEADLVRYRELEALLRTTLVSAEKSAVSLREQAGREADLIVEEARSEAR
                     AITRTARADHDRLVTEVRRMKSLLHSALALVDEEPPGKTAEAEAA"
     gene            3694..4062
                     /locus_tag="VF225_07815"
     CDS             3694..4062
                     /locus_tag="VF225_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7145202.1"
                     /translation="MNAIETDRFRETLLEERQRVEAAIQNLHDEHPGTLAEDSGEETA
                     YDNHLADTATETYDRELDYTLEENSGHVLGEIDAALKRIEEGTYGTCTNCDRPIAVER
                     LEALPWATLCIDCQRDRERR"
     gene            4059..4646
                     /gene="lspA"
                     /locus_tag="VF225_07820"
     CDS             4059..4646
                     /gene="lspA"
                     /locus_tag="VF225_07820"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7145203.1"
                     /translation="MTERLSPRGADIRVGSTTDGLTPISVAERRLGASWPQWLSLGSV
                     ALAGVAADQLTKSIVTSRLDLNDQVHVVGPFSIHHVTNSGIAFGLFASATSIVILLTG
                     LAVAWMLYFFARSGSRHPVLPVALGLVIGGSVSNLTDRVRLGHVTDFLDFKYWPAFNL
                     ADSFIVVGVAALLLALVASDRNAGRPQRVREATRS"
     gene            4627..5523
                     /locus_tag="VF225_07825"
     CDS             4627..5523
                     /locus_tag="VF225_07825"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7145204.1"
                     /translation="MKQRVPDEAAGERLDRFLASLPEVGSRGVAERLLETGTVLVDGR
                     PLDKSRRLSGGEELEFEPPEPEVTTLVPEQMDLRIAYEDEHLMVVDKPAGLVVHPASG
                     HATGTLVQGLLGHDIAGGDAARPGIVHRLDRDTSGLMVVARSEEAHARLQNLIRNRAL
                     ERHYTALVVGRPRSRTGRIEAPIGRDRRDPLRHSLDTDKPREAVTHFEVRELLPRHTL
                     LDVRLETGRTHQIRVHLAAVDLPVAGDAVYGRPRELDLERQFLHAGRLAFPHPFTEQR
                     VDVESPLPPDLAAALERARRLS"
     gene            5660..6508
                     /gene="rpsB"
                     /locus_tag="VF225_07830"
     CDS             5660..6508
                     /gene="rpsB"
                     /locus_tag="VF225_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7145205.1"
                     /translation="MSVVSMRELLEAGVHFGHQTRRWNPKMRRFIFTERGGIYIIDLQ
                     QTLQLLEDANAFARNVAERGGSILFVGTKKQSQGAVEEQAKRVNMPYVNHRWLGGLLT
                     NWRTISDRIDRLHELRRLKEEGQLDLLPAKERISMLNELEKLDENLGGVADMKRQPDA
                     VFIVDLKKEQLAVREARRLGLPVVALVDTNCDPDEADYVIPGNDDAIRSCDLIVRVIA
                     DGIAAGQQKATPADFTPAKNGAAAPAEEQASVPEGDSDEVAPQEEPVAEVAPAAEAKP
                     EEVPAE"
     gene            6505..7308
                     /gene="tsf"
                     /locus_tag="VF225_07835"
     CDS             6505..7308
                     /gene="tsf"
                     /locus_tag="VF225_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7145206.1"
                     /translation="MTGISAALVKELRDQTGAPMMDCKRALVDTNGDLEAAKRLLRER
                     GMASAEKRAGRATTEGKVGYRIGDDARRGTMVAVGCETEPVSNNDEFLAFAKKVLEAV
                     EADGPDAAEGLDEERKLLSGKLGENIVVAGTARFEAVDGGLIEGYAHPPANKLGVLVQ
                     VRGGSGDIARKIAMHIAASAPQWVGREDVPDDVVQSEREIFANSDEVLSKPDQARGKI
                     VEGMLNKRFFADRVLTDQPWIHDTGKTVGQALSEKGAEVLEFERFALTG"
     gene            7305..8078
                     /gene="pyrH"
                     /locus_tag="VF225_07840"
     CDS             7305..8078
                     /gene="pyrH"
                     /locus_tag="VF225_07840"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7145207.1"
                     /translation="MTVQPEVAIAGTGDGSPVFRRILLKLSGESLMGDEEYGMDRATI
                     DAIAEEIVDVQASGVETGIVVGAGNIYRGMAAAAEGMDRATADYSGMLATVLNSLALQ
                     DALEDRGADTRVLSALEVKEVAEPYIRRRAIRHLEKGRIVIFAAGTGNPFFTTDTAAA
                     LRALEVSADAILMAKHGTAGVYGNDPRVDPDAEFLPELTHLEAIERGLKVMDTTALSL
                     CMDNNLPIYVFALADGNIRRVIAGERIGTIISTPAKEER"
     gene            8078..8638
                     /gene="frr"
                     /locus_tag="VF225_07845"
     CDS             8078..8638
                     /gene="frr"
                     /locus_tag="VF225_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7145208.1"
                     /translation="MATPDQLIQDAQKRMDASVEHTKTEFNTVRTGRASAALLDRVQI
                     DYYGQQTPLKQLATINVPEPRMLTIQPFDPSSIKAIEKALLESDLGLTPSNDGKLIRL
                     PMPQPTEERRKELVKVVRKIAEEGRIAVRNVRRDAVQHLKDLAKGEVGQDDEHRAEER
                     VQKLTDEHTKTIDDLLKHKEAEIMEV"
     gene            8689..9429
                     /gene="uppS"
                     /locus_tag="VF225_07850"
     CDS             8689..9429
                     /gene="uppS"
                     /locus_tag="VF225_07850"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7145209.1"
                     /translation="MNLLTKIKTLRHVRPAPEPDLPDVAHAVAIIMDGNARWARKRGL
                     PIAAGHRAGTKALRRTVESAIDLGVDSLAVYAFSTENWNRPPDEVELLMEIFTETIER
                     ELPDLVRQGVRTRFIGRRDRAPDALQQRMAALEGETEGNRTLNLWIAFDYGGRAEIVD
                     AANRAVAGGQEVDEETFATYLNAPGMPDPDLLIRTSGELRISNVLLWQLAYAELVFVD
                     TLWPDFGKEELRAALDDYASRRRRFGGR"
     gene            9429..10226
                     /locus_tag="VF225_07855"
     CDS             9429..10226
                     /locus_tag="VF225_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX7145210.1"
                     /translation="MAPLVSRVLVALVGLPLVLLVVWAGGWWLFALAAVAAVLALHEY
                     ALMIRSLRPVILAAYTGAVLSLVGAHLGGLQWTMAGFLSTMVLAFLLHWIATTRQSAT
                     VAIASTVLGAGWIGLCLAHLLLLREIPSEGELATFTVLLAVWAGDIGAFFAGRLIGRH
                     KLAPALSPGKTWEGFLFGTAATIFVAFVALYKQHYLSIWQSIVLGVVIAIAAPLGDLF
                     ESALKRDMQVKDSGRLLAAHGGVLDRIDSLLFAVVASYYLLRAFNAA"
     gene            complement(10223..10699)
                     /locus_tag="VF225_07860"
     CDS             complement(10223..10699)
                     /locus_tag="VF225_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145211.1"
                     /translation="MIRRGAAADAEGVARVQVETWQAAYAHALPSEQLQALSIEEAVE
                     RHRLWPPTFVAEHEGQIVGFVSVGSSRDPDTDAELFAIYVHPEHWGTGVGRTLIESGE
                     EELRTLGHEEAILWVLDDNPRARRFYELAGWSVDGAAREIRLFGFDISEVRYAKRL"
     gene            10723..>11580
                     /locus_tag="VF225_07865"
     CDS             10723..>11580
                     /locus_tag="VF225_07865"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF020025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7145212.1"
                     /translation="MKRIALLGATGSIGRQALEIIDAVPELELCAAASGLQPIDGLAP
                     LTQTGGDLTELLEQAQPDLVLNAVVGFAGLPATLWALERGVDLALANKESLVAAGELA
                     LAAQRRGGGRLIPVDSEHSGAFQCLEGRSVDQVHSLVLTASGGPFRGRVRDELEDVTP
                     AAALAHPTWNMGPKITVDSATLANKGLELIEAHFLFGLPYDQIEVVVHPTSVVHALVR
                     LRDGATLAHLGYPDMRVPISYALTYPERSATPISPLDLAGGMTLEFEPPDLETFPLLA
                     HARTAGEQGG"
BASE COUNT         1962 a         3865 c         3893 g         1860 t
ORIGIN      
        1 cttccacggc gccgaggact tgaggaacgg cgtcctgcgc accgtgcgat cgcggagctt
       61 cgccgaggac ccgctccgcc tcgttcgtgg cctgcgcctg gtttcgcagc tcgggctcga
      121 gccggaggcg gaaacgctgc agcagatgcg cgccgaggcc tcgagcgtcg cgctcgtctc
      181 gggcgagcgc gtcggaggtg ggctccactc cgacggactg ggcgagttgt cgaagctcct
      241 gctcggacgc gagcctgcgc gcgcgctgcg gctggcccgg gacaccggcg tcctcaccgc
      301 gctcctgccc gagttcgagc cgtcgatcgg gttcgagcaa gacagcgaac ggcagcatct
      361 gccgcttgac gagcacacct tcgcggtcgt ccaggctgca gccgacgcgg acatgccgct
      421 cgcggtacgg ctcggggcgc tgttccatga cctcggcaag ccgcgggcga acgggaagca
      481 cgcagaacgc ggcgccgagc ttgcggctgc cgcaatgcga cgactccgtt atccaacgaa
      541 gttgcgcacc tatgtgactc ggctcgtgcg cgcgcacgcc ttctcgctgg aggacgtcga
      601 cgagctgttc gcgcgccgct tcctccggga gcacggagac gagctcgcat tcgacctcgt
      661 cttgcacaag gaggccgacc tccgcggcaa gaatgtctct caggacgagc tggccgccgc
      721 cgcgaggcta cgcgaactct tggagcagga acgaagccaa cctcaccacc tgaccgatct
      781 cgccgtggat ggatcggatc tgatcgagct cgggttcgct gaagggccgg tcctaggccg
      841 gacactcgag tcgctgctcg acgcagtcgt cgacgagcct tcgctcaaca cgcgcgacca
      901 gttgctcgaa cgcgcgcgca tgcagctttc atgacggtcc cgttgatccg ctgggacgcg
      961 cccggcccat acgcggtcgc cttctccaca cgactgggcg gggtcagcgt gggcgcgtac
     1021 gagtcgctca acctcggccg gagcactcgc gacgattcag cgaacgttga cgagaaccgt
     1081 cgtcggttgt ccgcgtcggt tggcgtcgag cccgagcggc ttgcactcaa ccggcaggtc
     1141 cacggcgcca cggtcaaccg cgccgaaaag ggtgagcgca cgcagaacgg ggacggtctc
     1201 tggacggacg agcccggtgt cccgatgttg gccttcagcg ccgattgcct cccgatcgcc
     1261 ctcgcgcgtg tcaacggcaa ccctggtctg gcgctcttgc atgcgggtcg cctggggctg
     1321 ctcgagggaa tcctggaggc gggcgtcggc gcactgcgtg gcccgatcgc agcgatcgtc
     1381 gggcccggga tcggtccctg ctgttacgag gtcggtgtgg acatccttgc tgtgtaccgg
     1441 gcacgcttcg gtcccaaagt catccacgga cgaaacctcg acctctggag cgccgccgag
     1501 catgtgctcc gcgaagcggg agtcgaggcc gtcgaacgcc tcgacgtgtg cacggcgtgc
     1561 gacgatcgct tcttctcgca tcgacgcgac ggaccgatca cgggacgaca gggagtcatc
     1621 ggttatgtca cctgagacga tccgcgcgaa ctacgaacgt atccgctccg aggtcggtga
     1681 gagtgtgacg gtggtggcgg cgacgaagta cgtcgcgctc gaagacatgc gcctgctcgc
     1741 cgaagcaggc gtcgaagtcg tcggagagaa ccgcgcacag gagctcgagc gcaagcacgc
     1801 ggtgtacggc gacacattca ggtggcactt catcggccac ctgcagtcga acaaggtgaa
     1861 ggtcgtgaat cgcagctgcg agctcgtgca ctcgctcgac tcggattcga ccgcacgtcg
     1921 tctcgctgtt cccgcgctcg tcgaggtgaa cctggccggc gagtcttcga agtcgggagt
     1981 tgctccggag gacgtgccgc gcttactcga cctccacggc gacgtgcgcg ggctgatgac
     2041 gatgccaccg gagacccgcg atcccgaggc gtcgcgtccc tacttcatcc ggttgaggaa
     2101 gttggccgaa gagcacggcc tgaaggagct ttcgatgggg acgagccagg actacagggt
     2161 cgccgccgaa gaaggcgcga cgttcgtccg cgtgggctca atcctgtacg gggagtaaca
     2221 tttcgcgcga gatgggcttt gctgacgtgt ggaacaagac cctcgtctac ttcggcatcg
     2281 ccgaggagga ggactgggac gaagaggcct acgtcacgga cgaggacatc cagcgcagct
     2341 atgccgatcg gccgaacgtc cgtcgcctgg cgccgcggcg gcgcgagcgt gagttcgacg
     2401 actggacgga tcccgagccc gcacccggca acgacaggac cacggtcctg cggccacgcg
     2461 tggcggcggt cccgtcgccg gcgtccgtac gcgttcacct ggtcgtgcct cggagcttca
     2521 acgacgcgca gcagatcgcg gacaagttca aggaatcgat ccctgtcatc ctcaacctgc
     2581 agagctcgga ccaggacctc tcgaagcgcc tgatcgactt tgccagcggg ctgacgtacg
     2641 ccctcgacgg cggcatgcag cgcgtggccg acaaggtctt cctgctgacg ccccgtaacg
     2701 tcgaggtgtc ggccgaggag cgcgcgcgcc tgctcgagcg cggcggcttc ttcaaccagg
     2761 cctgagcaag gaagacgggc ctttacgccg aactgggtga aatggccgcc ggtgccatgc
     2821 tcggcttgct ctacgacgcc atcgacaacg tcgtcggctt cgtgcaagtt tttatcaccg
     2881 tctattcgct gctgatcctc gcctacatca tcacctcgtg ggtacggctt ccttactcga
     2941 cgtggctgaa caggatccag cgattcctct acgacgtctg cgacccgtac ctgcggctgt
     3001 ggcgtcgcat cctgccgacg ttcgggccgc tcgacctctc gcccgtggtc ggcgtggcat
     3061 ttctctacat cctgtcgagg gtcatcgtaa gcatcctcaa cagcttccac tgaaaggggg
     3121 agactgtggc gctcactccc gttgaaatcc gacatctgaa ggccgcgcgg agcctcgtcg
     3181 ggggctacaa gaggacggcc gtcgacgcgc tcatggacga gatcgtcgcg agcttcgagg
     3241 acgtctggcg cgaacgtgcg gaccttgcag acaagctcga gcaactcgag gcggatctcg
     3301 ttcgctaccg cgagctcgag gcgctcctcc gtacgacgct cgtctctgcc gagaagtccg
     3361 cggtcagtct cagggagcag gccgggaggg aggccgatct catcgtcgag gaggcgcgct
     3421 cggaggcccg cgcgatcacg cggacggcgc gcgcggatca cgaccggctc gtgaccgagg
     3481 tccggcgaat gaagtccctg ctccactcgg cgctcgcgct cgtggacgaa gagccgccgg
     3541 ggaagactgc cgaggccgag gcggcctgag cggactacgg agctagactc cctgcatgga
     3601 agcggtttcc acgcgcctgc ggctgcgtgc cgggctcgac tcgactcagg tcgaaaggcg
     3661 tctctccacg gccgccggaa aggaacgctg aacttgaacg caattgaaac cgaccggttc
     3721 cgcgagactc tgctcgagga gcgtcagcgc gtcgaggcgg cgatccagaa cctccacgac
     3781 gagcatcccg gcacgctggc ggaggattcg ggagaagaga ccgcttacga caatcatctt
     3841 gccgatacgg cgacggagac ctacgaccgc gaactcgact acacgctcga agagaactcc
     3901 gggcacgtcc tgggggagat cgacgccgcg ctaaagcgga tcgaggaagg cacgtacggg
     3961 acttgcacga attgcgaccg gccgatcgcc gtcgagcgcc tcgaggcgct gccctgggcg
     4021 acgctgtgca tcgactgcca gcgcgaccgg gagcgtcgtt gaccgagcgg ctctcgccgc
     4081 ggggggcgga catccgcgtc ggctcgacga cggacggcct gacgccgatc tccgtcgccg
     4141 agcggcgact cggtgcaagc tggccgcaat ggttgagcct tggctcggtg gcgcttgccg
     4201 gcgtcgcggc ggatcagttg acgaagtcga tcgtgacgag ccgtctggac ctcaatgacc
     4261 aggtgcatgt cgtcggcccg ttctcgatcc accacgtcac caactcgggc atcgccttcg
     4321 gactcttcgc gagcgcgacg tcgatcgtca ttctgctgac gggtctggca gtcgcgtgga
     4381 tgctctattt cttcgcgcgc tccggctcgc gtcatcccgt cttgccggtt gcgctcgggc
     4441 tcgtgatcgg gggcagcgtc tcgaatctga ccgaccgcgt ccggctcggg catgtgacgg
     4501 acttcctcga cttcaagtac tggccggcct tcaacctcgc ggacagcttc atcgtcgtcg
     4561 gcgtagcggc tctgctgctg gcgctcgtgg cctcggaccg aaacgccggc cggccgcagc
     4621 gcgtacgtga agcaacgcgt tcctgacgag gcggccggcg agcggctcga ccgcttcctc
     4681 gcctcgctgc cggaggttgg ctcgcggggc gtcgcggagc ggctgctcga aacggggacg
     4741 gtgctcgtag acggccggcc gctcgacaag agccggcgac tctcgggcgg ggaggagctg
     4801 gagttcgagc cgcccgagcc cgaggtcacc acgctcgtgc cggagcagat ggacctgcgc
     4861 atcgcctacg aggacgagca cctcatggtg gtggacaagc ccgccggcct cgtcgtgcac
     4921 ccggcctccg ggcacgcgac gggcacgctc gtccagggtc tgctagggca cgacatcgcc
     4981 gggggcgacg ccgctcgtcc aggcatcgtg caccgcctcg accgcgacac ctccggcctg
     5041 atggtcgtcg cgcgctcgga ggaggctcac gcgcgtctgc agaacctcat tcgcaaccgc
     5101 gcactggaac gccactacac cgccctcgtg gtgggaaggc cccgctcacg caccggtcgc
     5161 atcgaggcgc cgatcgggcg cgaccgccgt gatcccctgc ggcattcgct cgacaccgac
     5221 aagccccgcg aggccgtcac gcatttcgag gtgagggagc tccttccccg ccacacgctc
     5281 ctcgacgtcc ggcttgagac cggccgcacg catcagatcc gcgtgcacct cgcggcggtc
     5341 gacctgccgg tagcaggcga cgccgtctat gggcggccgc gcgagctcga tctggagcgc
     5401 cagttcctcc atgcgggccg gctcgccttc ccgcacccgt tcacggagca acgcgtggac
     5461 gtcgagtcgc cgctgcctcc cgatctagct gcggctctcg aacgcgccag gcgcctttcg
     5521 tagtcgctag aatgcagcgg tttttcgcac ccgacccggc ggatcagctt gggtggcggt
     5581 gccggcgttc gacggatgcc ggttccagcc aggccccacc ggcagcaatc acatcaacca
     5641 gaaaggggac ctgatcccga tgtccgtcgt ctctatgcgc gagctactgg aggctggagt
     5701 ccacttcggc caccagacgc gacgctggaa cccaaagatg cgccgcttca tcttcacaga
     5761 gcgcggtggc atctacatca tcgatctgca gcagacgctt cagctgctcg aagatgcgaa
     5821 cgccttcgct cggaacgtcg ccgagcgtgg cggctcgatc cttttcgtgg gcaccaagaa
     5881 gcaaagccag ggcgccgtgg aagagcaggc gaagcgtgtc aacatgccgt acgtcaatca
     5941 tcgctggctc ggcggcctgc tcacgaactg gcgcaccatc tccgaccgta tcgaccgcct
     6001 gcacgagttg cgccgcctga aggaggaggg ccagctcgac ctgctgccgg ccaaggagcg
     6061 catctcgatg ctcaacgagc tcgagaagct cgacgagaac ctcggcgggg tggccgacat
     6121 gaagcgccag cccgacgccg tgttcatcgt cgacctgaag aaggagcagc tcgccgtccg
     6181 cgaggctcgg cggctcgggc tgcccgtcgt cgcgctcgtc gacacaaact gcgacccgga
     6241 cgaggccgac tacgtgattc cgggcaacga cgacgcaatc cggtcctgcg acctgatcgt
     6301 ccgcgtgatc gcagacggca tcgcagccgg gcagcagaag gcgacgccgg ccgacttcac
     6361 gcccgcgaag aacggagcag cggcgccggc cgaggagcag gcgtccgtcc cggaaggcga
     6421 ttccgacgag gtggctccgc aggaagagcc cgtcgccgaa gttgcgcccg cagcggaggc
     6481 caagccggag gaggtgcccg ccgaatgacg ggcatcagcg cggcactcgt caaggagctt
     6541 cgcgaccaga ccggtgcgcc gatgatggac tgcaaacgcg ctttggtgga caccaacggc
     6601 gacctcgaag cagcgaagcg gcttctgcgc gagcgcggca tggcgagcgc cgagaagcgc
     6661 gcaggccgcg ccaccaccga gggtaaagtc ggctaccgga ttggcgatga cgccaggcgg
     6721 ggaacgatgg tcgcggtcgg ctgcgagacc gagccggtat cgaacaacga cgagttcctt
     6781 gcgttcgcga agaaggtcct cgaagcggtc gaagcagacg gcccagacgc cgccgagggc
     6841 ctcgacgaag agcggaagct gctctccggc aagctcggcg agaacatcgt cgtagcaggg
     6901 accgcacgct tcgaggcggt cgacggcggg ctgatcgagg ggtacgcgca cccaccggcg
     6961 aacaagctcg gcgtgctcgt gcaggttcgt ggcggctcgg gcgacatcgc ccggaagatt
     7021 gccatgcaca tcgcggcgtc ggccccgcaa tgggtcggca gggaagacgt gcccgacgac
     7081 gtcgtgcagt ccgagcgcga gatcttcgcg aactcggacg aggtcctctc gaagcccgac
     7141 caggcccgcg gcaagatcgt cgaaggaatg ctcaacaagc gcttcttcgc cgatcgggtg
     7201 ctgacggatc agccctggat ccacgacacg gggaagacgg tgggtcaggc gctctccgag
     7261 aagggcgccg aggtgctgga gttcgagcgg ttcgccctga cgggatgacc gttcagccag
     7321 aggtagcgat cgcagggacc ggcgacggca gcccggtctt ccggcgcatc ctgctgaagc
     7381 tctccggcga gtcgctcatg ggcgatgagg agtacgggat ggatcgagcg accatcgacg
     7441 cgatcgcgga ggagatcgtc gacgtccagg cctcgggggt cgagacggga atcgtggtcg
     7501 gtgccggcaa catctaccgc ggcatggccg ctgctgccga gggcatggac cgtgcgacag
     7561 ccgactactc cgggatgctc gcgacggtcc tgaactcgct ggcgctgcag gatgcgctgg
     7621 aggaccgagg tgccgacact cgcgtgctct cggcgctgga ggtgaaggag gtcgccgagc
     7681 cgtacatccg gcgacgggcg atccggcatc tcgagaaggg gcgcatcgtg atctttgccg
     7741 ccggcactgg caacccgttc ttcaccacgg acacagccgc ggcactccga gccctggagg
     7801 tcagcgcgga cgccatcctg atggccaagc acggcacggc cggtgtctac ggcaacgatc
     7861 cccgcgtcga ccccgacgcc gagttcctcc ctgagctcac gcatcttgag gcgatcgagc
     7921 gcggactcaa ggtgatggat accactgcgc tgtcgctgtg catggacaac aatctgccga
     7981 tctacgtctt tgcccttgcc gacgggaaca ttcgccgcgt gatcgccggc gagcgcatcg
     8041 gcacgatcat ctcgacgccc gcgaaggagg agcgctgatg gccacccccg accagctgat
     8101 ccaggacgcc cagaagcgga tggatgcgag cgtcgagcac acgaaaaccg agttcaacac
     8161 tgtccgcacc ggccgcgcgt cggccgcgct cctcgatcgc gtgcagatcg actactacgg
     8221 ccagcagaca ccgctcaagc aacttgccac gatcaacgtg cccgagccga ggatgctgac
     8281 gatccagcct ttcgatccga gctccatcaa ggcgatcgag aaagcgctgc tggagtcgga
     8341 tctcggcctg accccgtcga acgacggcaa gctcatcagg ctcccgatgc cgcagccgac
     8401 cgaggagcgc cgcaaggagc tggtcaaggt cgtacgcaag atcgcggagg agggacggat
     8461 cgcggtgcga aacgttcgcc gggacgctgt ccagcacctg aaggacctgg cgaagggcga
     8521 ggtcggccag gacgacgagc accgagcgga ggaacgcgtg cagaagctga ccgacgagca
     8581 cacgaagacg atcgacgatc tcctgaagca caaagaggcc gagatcatgg aggtttgacc
     8641 gcggtcttgc gaaagacctt cgcttcgctc ggtaaagagg aaaaatccat gaacctcctc
     8701 accaagatca agacactgcg gcacgtccgg cccgcgccag agcccgacct cccggacgtc
     8761 gcgcacgcgg tggcgatcat catggacggg aacgcgcgct gggcccgcaa gcgcggcctt
     8821 ccaatcgccg ccggccaccg cgccggcacg aaggcgttgc ggcgcacagt cgagtccgcg
     8881 atcgacctcg gggtcgactc gctcgcggtc tacgcgttct cgacggagaa ctggaaccgg
     8941 ccgccggacg aggtggagct cctgatggag atcttcacgg agacgatcga gcgcgagctg
     9001 ccggatctcg ttcgtcaggg agtgcgcacg cgtttcatcg gccgccgcga ccgtgcgccg
     9061 gacgcgctgc aacagcgcat ggcggccctc gagggcgaga cggaagggaa ccgcacactc
     9121 aatctctgga ttgcgttcga ctacggcggg cgcgccgaga tcgtcgacgc ggcgaaccgc
     9181 gcagtcgcgg gcggccagga ggtcgacgag gaaacattcg cgacttacct caatgcacct
     9241 gggatgcccg atcccgacct cttgatccgt acgtcgggcg agctgcggat ctccaacgtc
     9301 ctcctgtggc aactcgccta cgcggagctc gtgttcgtcg acacgctctg gcccgacttc
     9361 ggcaaggagg aactccgcgc cgctctcgac gattacgcga gccgcaggcg ccgcttcggc
     9421 ggacgttagt ggcccctctg gtttcgcgag ttctggtcgc gctcgtcggc ctgccgctcg
     9481 tgctgctcgt cgtctgggcg ggcggctggt ggctcttcgc gttggccgca gtagccgcgg
     9541 tgcttgcctt acacgaatat gcgctcatga tccggtcgct gcggccggtc atcctggcgg
     9601 cctatacagg agccgttctg tcgctggtcg gggcacacct gggcggcctc cagtggacga
     9661 tggcgggctt cctcagcacg atggtgctcg cgttcctgtt gcactggatc gccaccacaa
     9721 ggcagtcggc aaccgtcgcg atcgcttcga cggtgctcgg cgcgggctgg atcgggctct
     9781 gcctcgcgca tctgctgctc ctgcgcgaga ttccgagcga gggcgagctc gcgacgttca
     9841 cagtgctgtt ggctgtgtgg gccggcgaca tcggcgcgtt cttcgccggg cggctgatcg
     9901 gccggcacaa gcttgcgccc gcgctgtcgc cggggaagac ctgggagggc ttcctgttcg
     9961 ggaccgctgc gacgatcttc gtcgcattcg tcgccctgta caaacagcac tacctctcga
    10021 tttggcagtc gatcgtgctc ggcgtcgtga tcgcgattgc cgcgccgcta ggcgacctct
    10081 tcgaatccgc tttgaagcgc gacatgcagg tgaaggacag cgggcgcctg ctcgcagcgc
    10141 acggcggcgt cctcgaccgg atcgactcgc tgctcttcgc agtggtggcg tcgtactacc
    10201 tgctgcgcgc tttcaacgcg gcttagagcc gcttcgcgta tctcacctcc gagatgtcga
    10261 agccgaagag acggatctcg cgggcggcgc cgtctaccga ccagcccgcg agctcgtaga
    10321 agcggcgtgc cctcgggttg tcgtccagca cccagaggat cgcctcctcg tgcccgagcg
    10381 tcctgagctc ctcctcgccc gactcgatga gcgtgcgccc gacgccggtc ccccagtgct
    10441 ccggatgaac gtagatcgcg aacagctcgg catcggtgtc cggatctctg ctcgagccga
    10501 cgctgacgaa gcccacgatc tggccctcgt gttctgcgac gaacgtcggt ggccacagcc
    10561 ggtgtctttc gaccgcctct tcgatggaca aggcctggag ctgctcagag ggcaaggcgt
    10621 gggcgtaggc ggcctgccag gtctcgacct gaacacgggc aacaccttcc gcgtcggcgg
    10681 ctgctccccg gcgaatcacc gcgtcgaacc tacacttcca gggtgaagag gatcgcgctt
    10741 ctcggcgcta caggctcgat tgggcgccag gcgctggaga tcatcgacgc ggttccggag
    10801 ctcgagctct gcgccgcggc ttcgggctta cagccgatcg acgggctggc cccgctgacg
    10861 cagaccggtg gcgatctcac cgagctgctc gagcaagcac agccggacct cgtgctgaac
    10921 gcggtcgttg gcttcgcagg cttgccggcg acgctctggg cgctcgagcg cggcgtcgac
    10981 cttgcactcg cgaacaagga gagcctcgtc gcggccggcg agctcgctct cgccgcacaa
    11041 cgtcgcggag gcggacggct cattccggtg gacagcgagc attcgggcgc gttccagtgc
    11101 cttgaaggcc gctcggtcga ccaggtgcac tcactcgtac tcacggcttc cggtggaccg
    11161 ttccgggggc gggtacgtga cgagctggag gacgtcactc cggctgccgc gctggcgcat
    11221 ccgacctgga acatgggccc gaagatcacc gtcgactccg caacgctcgc caacaagggc
    11281 ctcgagctga tcgaggctca cttcctcttc gggctcccgt acgaccagat cgaggtcgtc
    11341 gtacatccga cctcggtcgt gcatgcgctg gtgcggttgc gtgacggtgc gacgctcgcg
    11401 catctcggct atcccgacat gcgcgtgccc atctcctacg ccctcacgta ccccgagcgc
    11461 agcgccacgc cgatttcgcc gctcgatctc gccggcggca tgacgctcga gttcgagcca
    11521 ccggatctcg agacatttcc gctgctcgcg cacgcgcgaa ccgcgggcga gcagggcggg
//
LOCUS       DASVEU010000192        14455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1490034,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000192 DASVEU010000000
VERSION     DASVEU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490034"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..321)
                     /locus_tag="VF225_07870"
     CDS             complement(151..321)
                     /locus_tag="VF225_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145213.1"
                     /translation="MRRIPLLVVIYLAIGVVVAYAENYFDNVDRTRQLISAILAVVLW
                     PLVLVGFDVEVT"
     gene            440..2086
                     /locus_tag="VF225_07875"
     CDS             440..2086
                     /locus_tag="VF225_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145214.1"
                     /translation="MRILLLLATALLVLAAAGCGGDDDDGDGGEGATGGELVFAGASD
                     PVVLDGALVSDGESLRPIDQMFEGLVSLAPGSTEIIPALAESWEANADGTAWTFRLRE
                     NVTFHDGEPFNAEAVCFNFDRWYNFKGPFQLESATYYWQTVFGGFSDGEEPSLYESCE
                     ATDESTVVINLTKASSSFLGALALTNFTFASPQALQEFGADEGTVDEETGFKPTGTYG
                     TEHPTGTGPFRFVSWTRGDRLVLERYEDYWGEKAKLDRLIFRPISDNAARLQALQAGD
                     IQGYDLVEPQDIETIEGDENLQVLDRPPFNVAYVGINQKMAPMNRLEVRQAIAHGLDR
                     QSVVDNFYAGRGEVAKAFMPPSLEGYADDVMEYEYDPEKSKELLRQAGLTLPVRIDFW
                     YPTDVSRPYMPDPKRNFEAFAASLNKAGFQVTPHSAPWSPDYLGRAQEGTAGHLHLLG
                     WTGDYGDPDNFIGTFFQTETGQFGFNNPELFDLLNRAETETDQAARIGLYEDANRMIM
                     EFLPGVPYAHTEPALAFTANVTGYTPSPVSLEPFSTVSME"
     gene            2126..3130
                     /locus_tag="VF225_07880"
     CDS             2126..3130
                     /locus_tag="VF225_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145215.1"
                     /translation="MLRFVVRRLLLLVPILVGLSLLVFLWIRALPGGPAQALLGERAT
                     AESIEQIEEQYGLDKPIHLQYFTYVSKIAQGDLGTSVTTRRPVVDELQQRFPATIELA
                     LAAMLFSITLGIPLGFIAAKRYGGAVDHGSLVLSLIGISIPVFFLAILLKYVFAVQLG
                     LLPTVGRISATIDLEHPTNFYVLDALIAGDMEALWDTLTHMVLPAIALGSIPLAVIAR
                     ITRAAVLDVQNEDYVRTARAKGLAPRVVDVRHIFRNAMLPIVTIIGLQVGLLLSGAVL
                     TETVFAWPGMGTWLVDAIEQRNYPVLQAGILFVAVVFVLVNLLVDISYALINPRIRVS
                     "
     gene            3130..4038
                     /locus_tag="VF225_07885"
     CDS             3130..4038
                     /locus_tag="VF225_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145216.1"
                     /translation="MSIAEAEARELQLEAPSGLWNEAWRRLRRNPGAIIGFVLVATFV
                     TCALFAPLIAPEDPRVGDLARLEGSCCPGPSAEHIFGVDQQGRDVFSRVIYGARYSLL
                     IGVVSVAVGLSIGMIIGAIAGYFGGWVDTLLMRLMDIMLSIPGLLLAIGIVAALGPGL
                     VQIMVAVGVTQIPIFARLLRGSILAAREADYVLAARSVGVPRRAILSSHLLPNAISPV
                     IVQGTLALATAIIEVAGLGFLGLGPQDPSTPEWGTMLTDTTRYLQTAPHLALIPGFAI
                     VISVLGFNLIGDGLREALDPRFRGRA"
     gene            4035..5048
                     /locus_tag="VF225_07890"
     CDS             4035..5048
                     /locus_tag="VF225_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145217.1"
                     /translation="MREPLLSVEDLQVQFWTARGTIYAVNGISFDIAPGETLGIVGES
                     GCGKSVTSLALLGILPRAGRVTAGSAHFEGRDLVKLSDDELRGIRGKDIAMIFQDPMT
                     SLNPVLTIGRQLREPLETHFGLDKRAAGDRSAELLDRVGIPSAKQRLRDYPHQFSGGM
                     RQRVMIAMALACEPKVLIADEPTTALDVTIQAQILDLLRELVAERDTALILITHDLGV
                     VAGTCERVNVMYAGMFMETGSAQQVFARPRHPYTLGLLQSIPRLDATRKTPLHPIEGN
                     PRDMLEPPQACPFQPRCRYEVDLSRQQVPPLEEIEPGHKVACFNPVPVDEWQKMREAV
                     VAR"
     gene            5045..6124
                     /locus_tag="VF225_07895"
     CDS             5045..6124
                     /locus_tag="VF225_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145218.1"
                     /translation="MSSMNGNLVELEDVKVYFPIKSGVVIDRHVGDIKAVDGVSLEIK
                     RGETLGLVGESGCGKSTIGRAIIRLYEPTGGRITFDGQDITSLGEAEMRPLRRRMQMV
                     FQDPYASLNPRHSVGRMISEPMRVHGVTRRRDAGRRVRELLQTVGLPADAASRYPHEF
                     SGGQRQRIGVARALALNPDFIVADEPVSALDVSIQAQIINLLENLQREFDLTYLFIAH
                     DLAVVRHISDRIAVMYLGKIVEISPAEELYDNPLHPYTISLLSAVPIPDPEVEAKREA
                     ILLPGDLPSPANPPPACRFHTRCPYIQPTRCTTEAPPLRKLSDGHLVACHWAEEIKAG
                     RIQPQEREAIVEQPLPVPVEEPPLV"
     gene            6193..6525
                     /locus_tag="VF225_07900"
     CDS             6193..6525
                     /locus_tag="VF225_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7145219.1"
                     /translation="MDAAERKEILSRYMDHSRRFEAVAQRRTTSGAGEVIPFTAPLKE
                     LEQEPTAREIEVLQLISEGLVNREIGHRLFLSEETVKSHVRHLLAKLQARSRAHAVAV
                     GFRRGLIA"
     gene            6725..8170
                     /locus_tag="VF225_07905"
     CDS             6725..8170
                     /locus_tag="VF225_07905"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7145220.1"
                     /translation="MNPPLRGTNGSQDVIPLMGEIQGGFAKIRRASIGDPLVLSYAGL
                     LALIAVTLGATLLASGVSVEASPFFIVALGLMGLLAERQVVQISANAQISVSVLPLLL
                     AATLYGPLDAMLVAVITLAGDFRRPYARWVTWTSIRALGGASAALAASAVAVSDPTFA
                     NLFAAVAAAAVAEALVDVALGAVTGALRGIASVSATLQLVKPVLLGTVPLYAPVVVLL
                     IYACREISEWSVVFFVVPAFAAHRFYSLYREEHDARRELNEVNARLQRANLSFATALV
                     ATLDARDRYTAGHSAAVAVYARDIARRLGLSEEEQRLAHLCGLVHDIGKIGLPPGLLE
                     KPGALTLDERRQMEQHSEIGERILANVEDYSEIARIVRHHHERMDGEGYPDRIAGSDI
                     PLLSRIIAVADAYNAMTSDRPYRDAMPSRVARLRLAQAVETQFDTNVVAAFEAILASA
                     GEDYRAGLNLLFDREAQAHEELGRPLIASVA"
     gene            complement(8167..10701)
                     /locus_tag="VF225_07910"
     CDS             complement(8167..10701)
                     /locus_tag="VF225_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7145221.1"
                     /translation="MAAEAVATRRRIIPRPRLTKLLDESPARIKLLVAPAGYGKTTLA
                     QQWLSTPPRQGLWYRGGPASADVAALAAGVSNLIGDLIPDAGGRMRKRIRSVGQPEDH
                     VDLLAALFAEDIQQWPSDVWLAIDDYQFAMESTASERFLELLVQDTPIRLIVTSRRRP
                     TWATARRIVYGELQEIDRRELAMAAEEAKSVIGRDDQDVIALLAHARGWPAVIGLAAT
                     TDHLRAPEHGLPPALYDYFAEELYQQVAPPLRLALCKLCLTRVITPDMARFLFGESYA
                     PALEVGCRLGILQTQGATYEMHPLIRTFLEAKLVSLEEANSFELAGQVVDHLTRTGAW
                     DDAFDVIDRFSVHDRLEALVSAAVEDIIETGRVTTLAQWLEFAAKHHLTAPILDFAEA
                     ELAFRHGRYGTAAALASNAARIADGAPEYGQRLRALILLRAGQSALLDSRETEALRHF
                     REARKIAPTGSLEREAAIGQFFSCVDLGLDEVAELLDDLEAISTRGSEDLVRLSIARM
                     LYAERTGGLADALNSAADVYPLLDRVTDPVITTSFLNSYGQSLGLMAWYETAIRLADE
                     AETVAREYRLDFVLSHVAILRGMAAIGLRDVSAAEDAIAHAEQGPEDPHITAGAATLR
                     ARLALLRGHPDQAFATLSTPLGRLPSDAMYAEYLSSRALAAAATGDFGTATELSNQVL
                     LAPRFARGGATTATLVKAVSVLESNPGAREAVAESLAFVLDGGRFDEFVAAYRTFPVL
                     LSAAFASASFRDVARSIVARANDVKLARRIGVEVTAPLIGPLGRLTPREREVLGLVAK
                     GLTNKQIAAHLVISQATVKVHVRHALEKLGATTRAEAAVMLSAVPP"
     gene            complement(10794..11060)
                     /locus_tag="VF225_07915"
     CDS             complement(10794..11060)
                     /locus_tag="VF225_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7145222.1"
                     /translation="MQEALTRIPPSITQRQLQVIELIAAGCSNEEVGERLGISPRTAK
                     AHCDVLRQKLGVARRRQIPVAYRLLTGKDPLSRSLGPVTLGSGD"
     gene            complement(11194..12438)
                     /locus_tag="VF225_07920"
     CDS             complement(11194..12438)
                     /locus_tag="VF225_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145223.1"
                     /translation="MDFELTDEQRLIQDTVRDFVDRKVLPNAVENDIEHRLDMSVIEG
                     MAELGLLGIVIPEEYGGAGLDFVAEALSCEEIERGEAAFRTLISVHVGLNSLSLLRYA
                     TEEQKQRYLVPQARGEKLACFGLTEPAAGSDVASMRTTARREGDTYVLNGQKNWISYA
                     SVADHQLVFAKTDPSAKHKGISAFVVERGWKGVSTRDTEHKLGIWAGSTGELFFENVE
                     VPAENLVGEEGQGFEIAMYGLDQGRFTVAAGACGVIRACLERSVEYARERETFGQPIG
                     KYQFVQDMIAEMVLGYETSKLLVMQAAWMKNEGRRNTRETSLAKWHATESAFKAAHLA
                     IQVHGAYGYSAEYGIERYFRNARAPIIYEGTTQVHKMMQAEHALGWRALNGRDGDVSP
                     LCSWAPALSGVHRAAGPVPTGA"
     gene            12482..13705
                     /locus_tag="VF225_07925"
     CDS             12482..13705
                     /locus_tag="VF225_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145224.1"
                     /translation="MTDSSINPRMATMTAIEEAMATATGVSFALSDEQRELRRLAREF
                     AEREIRPKAAEYDEHQTHPADVVAKAHEVGLMNPHIPEAYGGLGLPGFEGMLIGEELS
                     WGCSGIAVSIVANTLGAAPVVLAGSEEQKIKWLPPLVEEPILCSFGLTEPNAGSDVSG
                     IQTTAVRQGDEYVINGSKMFITNAGHADWLVVFASTDKSKGHRGLSAFVVPTELDGVV
                     VEKHLDKMGQRATDTSAIAFSDVKVPVENRLGEEGEGFKIAMRTLDFTRPGTAAGAVG
                     VAQAAFDYSVEYAKTRVQFGQPIAMNQGINFLVADMATEIEAARLLVWQAAWLLDQGK
                     RATLQSSFAKRFAADTAMKVTTDAVQIFGGYGYIKEYPVEKLMRDAKLFQIYEGTSQI
                     QRLVIAREIFLPRDD"
     gene            13695..>14455
                     /locus_tag="VF225_07930"
     CDS             13695..>14455
                     /locus_tag="VF225_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7145225.1"
                     /translation="MTIEIDLSGRVALVTGGSRGLGRADALTLARAGADVVIADILVE
                     SDDSAAEVYGPLAQAAAARGLVQTEATAEEIRGLGRRSLALKCDVTDSDQVEAAVART
                     VEGLGSVDILVNNAGTLDHLGQLEHQVPELWERDLRVNLTGAFNCSRAVWPHMKERGW
                     GRIVNMASVAGTLGGFGQASYSTTKAGLIGLTKTLALEGARHGITANAIVPGIIGTEA
                     FHFGNPDMNERMVKRTALRRTGDPQDIANAIAFLCS"
BASE COUNT         2430 a         4839 c         4729 g         2457 t
ORIGIN      
        1 gctgtagagg taaccgacgc ggaacgggct cagcccgttc cgccgcgtcg gcccatcgag
       61 aaggaagcgg cgcagccgcg gtaggccggg cggcaaagcg ccgcccggct tcatacggtc
      121 gtcgaaagtc gtgagacttt cgacgaaacg ctaggtgact tcgacgtcga agccgaccag
      181 gacgagcggc cagagcacca cggcgaggat cgccgagatg agctgcctcg tccggtcgac
      241 gttgtcgaag tagttctccg cgtaggcgac gacgacgccg atcgcgaggt agatcacgac
      301 gaggagtggg atgcggcgca tgcttcctcc gtacccgccg cttaacgcca accaaacgac
      361 gcaagtgacg tcggttctta tactttccgc gatttcccct ggaacgacag ataggaggct
      421 gacggtgggt cggacgtcaa tgaggatttt gctgctgctt gcgacggcat tgctggtgct
      481 cgcggcggcc ggctgcggcg gagacgacga cgatggcgac ggcggtgaag gcgccacggg
      541 cggagagctc gtcttcgcgg gggcttccga cccggtcgtc ctcgacggcg cgctcgtctc
      601 ggacggcgag tcgctccgtc ccatcgacca gatgttcgag ggtctcgtca gcctcgcacc
      661 cggctcgacc gagatcatcc cggccctggc cgagagctgg gaggcgaacg cggacggaac
      721 cgcgtggacg ttcaggctgc gcgagaacgt cacgttccac gacggcgagc cgttcaatgc
      781 ggaggcggtc tgcttcaact tcgaccgctg gtacaacttc aagggtccgt tccagctcga
      841 gagcgcgacc tactactggc agaccgtctt cggcggtttc tccgacgggg aggagccgag
      901 cctctacgag agctgcgagg cgaccgacga gtcgaccgtc gtcatcaacc tgacgaaggc
      961 gtcgtcgtcc ttcctcgggg ccctggcgct gacgaacttc acgttcgcga gcccgcaggc
     1021 gctccaggag ttcggcgccg acgaggggac cgtcgacgag gagaccggct tcaagccgac
     1081 gggcacctac ggcaccgagc atcccaccgg cacaggcccg ttcaggttcg tgtcctggac
     1141 gcgcggtgac cgcctcgtgc tggagcggta cgaggactac tggggcgaga aggccaagct
     1201 cgaccggctg atcttccggc cgatctccga caacgcggcc cggctgcagg cgctccaggc
     1261 cggggacatc cagggctacg acctcgtcga gccccaggac atcgagacga tcgagggcga
     1321 cgagaacctc caggtcctcg accggccgcc cttcaacgtc gcgtacgtcg gcatcaacca
     1381 gaagatggcg ccgatgaaca ggctcgaggt ccggcaggcc atcgctcacg ggctcgaccg
     1441 tcagtcggtc gtcgacaact tctacgccgg ccgcggcgag gtggcgaagg cgttcatgcc
     1501 tccgtcgctc gagggctacg cggatgacgt catggagtac gagtacgacc cggagaagtc
     1561 gaaggagctg ctccggcagg ccgggctcac gctgccggtg aggatcgact tctggtaccc
     1621 gaccgacgtc tcgcggccat acatgcccga cccgaagcgc aacttcgagg cgttcgcggc
     1681 cagcctgaac aaggccggct tccaggtgac gccgcacagc gctccatgga gcccggacta
     1741 cctcggccgc gcgcaggagg gcacggccgg ccacctccac ctgctcgggt ggacgggcga
     1801 ctacggcgat ccggacaact tcatcggcac cttcttccag accgagacgg gccagttcgg
     1861 gttcaacaac cccgagctct tcgacctgct caacagggcc gagaccgaga ccgaccaggc
     1921 ggcgcgcatc ggcctctacg aggacgcgaa ccggatgatc atggagttcc tgccgggcgt
     1981 gccgtacgcg cacaccgagc cggcgctcgc cttcaccgcg aacgtcacgg gctacacgcc
     2041 gagccccgtg tcgctcgaac cgttctcgac cgtgtcgatg gaatagcgga accgagccag
     2101 agccggcgga cgacgtcgtg acgtaatgct ccgattcgtc gtccgccggc tgctgctcct
     2161 ggtcccgatc ctcgtcggcc tctccctgct cgtcttcctc tggatccggg cgctgcccgg
     2221 cggtcctgcg caggcgctcc tcggcgagcg ggcgacggcc gagtcgatcg agcagatcga
     2281 ggagcagtac gggctggaca agccgatcca cctccagtac ttcacgtacg tctccaagat
     2341 cgctcagggc gatctcggga cgagcgtcac gacccgccgg cccgtggtcg acgagctgca
     2401 gcagcggttc ccggcgacga tcgagctcgc cctggcggcg atgctgttct ccatcacgct
     2461 cgggatcccg ctcgggttta tcgcggccaa gcgctacggc ggcgccgtgg accacggaag
     2521 cctggttctg tcgctgatcg ggatctcaat cccggtcttc ttcctcgcga tcctgctcaa
     2581 gtacgtgttc gcggtgcagc tcgggctctt acccacggtg ggccggatct ccgcgacgat
     2641 cgatctcgag cacccgacga acttctacgt cctggatgcg ctgatcgcgg gcgacatgga
     2701 ggcgctctgg gacacgctga cgcacatggt cctgccggcg atcgccctgg gctccatccc
     2761 gctcgccgtg atcgcgcgca tcacgcgcgc ggccgtgctc gacgtccaga acgaggacta
     2821 tgtccgcacc gcgcgcgcaa agggcctcgc gccgcgcgtc gtggacgtcc gccacatctt
     2881 ccgcaacgcc atgctgccga tcgtgacgat catcgggctc caggtcggcc tgctgctctc
     2941 aggcgcggtg ctgaccgaga ccgtcttcgc ctggcccggc atggggacgt ggctcgtcga
     3001 cgcgatcgag cagcgcaact acccggtgct gcaggccggg atcctcttcg tggcggtggt
     3061 cttcgtcctc gtaaacctcc tcgtggacat ctcctacgcg ctgatcaatc cgaggatccg
     3121 ggtctcctga tgtcgatcgc cgaagccgaa gcacgagagc tccagctcga ggcgccgagc
     3181 ggcctctgga acgaggcgtg gcgccgcctg cgccgcaacc ccggggcgat catcggcttc
     3241 gtcctcgttg cgaccttcgt cacctgcgcc ctcttcgcgc cgctgattgc cccggaggat
     3301 ccacgcgtcg gcgacctcgc ccgtctcgaa ggctcgtgct gcccgggtcc ctctgccgag
     3361 cacatcttcg gggtcgacca gcagggccgc gacgtcttct cccgcgtcat ctacggcgcg
     3421 cgctactcgc tgctgatcgg cgtcgtgtcc gtcgccgtcg ggctctcgat cgggatgatc
     3481 atcggcgcga tcgccggcta cttcggcggc tgggtcgaca cgctcctgat gcgcctgatg
     3541 gacatcatgc tgtcgatccc gggcctgctc ctcgcgatcg ggatcgtcgc cgcgctcggg
     3601 cccggcctcg tgcagatcat ggtcgcggtc ggggtcacgc agatcccgat cttcgcgcgg
     3661 ctcctacggg gctcgatcct cgcggcgcgc gaggccgact acgtcctcgc cgcgcgctcg
     3721 gtcggcgtgc cgcggcgggc gatcctctcc tcgcacctgc ttccgaacgc gatctcgccc
     3781 gtgatcgtgc agggaaccct ggccctggcc acggcgatca tcgaggtcgc gggactcggg
     3841 ttcctcgggc tcgggccgca ggatccgtcg acgccggagt gggggacgat gctgaccgac
     3901 acgacgcggt acctccagac cgcgccgcac ctggcgctca tccccggctt cgcgatcgtc
     3961 atctcggtgc tcggcttcaa cctgatcggc gacgggctcc gcgaggcgct cgacccccga
     4021 ttccggggac gcgcgtgagg gagccgctcc tctcggtcga ggaccttcag gtccagttct
     4081 ggacggcgcg cgggacgatc tacgccgtca acggcatctc gttcgacatc gcgccgggcg
     4141 agacgctggg catcgtcggc gagtccggct gcgggaagag cgtcacgtcg ctcgcgctgc
     4201 tcgggatcct gccgcgagcg ggacgggtga cagccggctc cgcccacttc gagggccgcg
     4261 acctggtgaa gctctccgac gacgagctcc gcgggatccg cggcaaggac atcgcgatga
     4321 tcttccagga cccgatgacg agcctgaacc cggtcctgac catcggccgg cagctccggg
     4381 agccgctcga gacgcacttc gggctggaca agcgcgccgc cggggaccgc tccgccgagc
     4441 tgctcgaccg ggtcgggatc ccgagcgcga agcagcgcct gcgcgactac ccgcaccagt
     4501 tctccggcgg gatgcggcaa cgcgtgatga tcgcgatggc gctcgcctgc gagccgaagg
     4561 tgctgatcgc cgacgagccc acgaccgcgc tcgacgtcac gatccaggcg cagatcctcg
     4621 acctgctccg cgagctggtc gccgaacgcg acacggcgct gatcctgatc acgcacgacc
     4681 tcggcgtggt cgccggcacg tgcgagcgcg tgaacgtgat gtacgcgggg atgttcatgg
     4741 agaccgggtc ggcgcagcag gtcttcgcgc ggccgcggca tccctacacg ctcggcctgc
     4801 tccagagcat cccgcgtctc gatgcgacgc gaaagacgcc gctccacccg atcgagggga
     4861 acccgcgcga catgctcgag ccgccgcagg cctgcccgtt ccagccgcgc tgccgctacg
     4921 aggtcgacct ctcccgccag caggtcccgc ccctcgagga gatcgagccc ggccacaagg
     4981 tcgcctgctt caacccggtt cccgtcgacg agtggcagaa gatgcgcgag gcggtcgtcg
     5041 cccgatgagc agcatgaacg gcaacctcgt cgagctcgag gacgtcaagg tctacttccc
     5101 gatcaagagc ggcgtcgtga tcgaccgcca cgtcggagac atcaaggcgg tcgacggtgt
     5161 ctcgctggag atcaagcggg gcgagacgct ggggctcgtc ggcgagtcgg gctgcggcaa
     5221 gtcgaccatc ggccgcgcga tcatccgcct ctacgagccg acgggcggcc ggatcacgtt
     5281 cgacggtcag gacatcacga gcctcggcga ggcggagatg cgcccgctca ggcggcggat
     5341 gcagatggtc ttccaggacc cgtacgcctc gctgaacccg cgccattccg tcggccggat
     5401 gatcagtgag ccgatgcgcg tgcacggcgt gacccgccgt cgcgatgcgg ggaggcgagt
     5461 gcgggagctc ctgcaaaccg tgggcctgcc ggccgacgcc gcttcgcgct atccgcacga
     5521 gttctccggc ggccagcgcc agcggatcgg cgtggcgagg gcgctcgcgc tgaacccgga
     5581 cttcatcgtc gccgatgagc cggtctccgc gctcgatgtc tcgatccaag cgcagatcat
     5641 caacctgctg gagaacctcc agcgggagtt cgacctcacg tacctcttca tcgcgcacga
     5701 cctggccgtg gtgcggcaca tctccgaccg gatcgccgtc atgtacctgg gcaagatcgt
     5761 cgagatctcg ccggccgagg agctctacga caacccgctc cacccgtaca cgatctcgct
     5821 cctctccgcg gttccgatcc cggatccgga ggtggaggcc aagcgcgagg cgatcctgct
     5881 cccgggcgac cttccgagtc cggccaaccc gccgcccgca tgccggttcc acacgcgctg
     5941 cccgtacatc cagccgaccc gctgcaccac tgaggcgccg ccgctccgga agctgtccga
     6001 cggccacctc gtcgcctgcc actgggcgga ggagatcaag gcgggccgga tccagccgca
     6061 ggagcgcgag gcgatcgtcg agcagccgct gccggtgccg gtcgaggagc ctccgctcgt
     6121 ttaggcgtct gttaagggca tcactcgaaa gggggagttc agtcttgtcc acagggcgcc
     6181 gatacgtggg tcgtggacgc tgctgagcgc aaagagatcc tcagtcgcta catggaccac
     6241 tcgcggcggt tcgaggctgt ggcccagcgg cggactacga gcggcgcagg tgaggtcatt
     6301 ccgttcactg ctccgctcaa ggagctcgag caggaaccga ccgcccggga gatcgaagtc
     6361 ctccagctga tctcggaggg cctcgtcaac cgggagatcg gccaccgcct gttcctgtcg
     6421 gaagagacgg tgaagtccca cgtgcgacac ctgctggcca aactgcaggc gcgctcgcgc
     6481 gcccatgcgg tcgcagtggg cttccggcgc ggcctgatcg cctagctagg cgaaccgcaa
     6541 gttcggattt ctgggagcca ccgctccgcc tatccacacc ctgtggacag atccctcaac
     6601 agggggatgc ccaggtatgt ggaaaagcag agactccgcg agcggcacct accagatgcg
     6661 aattgcaccg gcgactgccg ctaccgacgt gcaagacgcc agcagagcga caagacggag
     6721 cttcatgaat cctcccttga ggggaactaa cggcagccag gatgttatcc cccttatggg
     6781 ggagatacaa ggcggattcg cgaagattcg ccgggcatcc atcggcgacc ctctcgtact
     6841 cagctacgcc gggctgttgg cgttgattgc ggtgacgctc ggtgcgacgc tcctcgcctc
     6901 cggtgtctcc gtggaggcat cgcccttctt catcgtcgct ctgggcctta tgggtttgct
     6961 agccgagagg caggttgttc agatcagtgc gaacgcccag atttcggttt ctgtgcttcc
     7021 gcttctgctg gccgcaacgc tctatgggcc actcgacgcc atgctcgtcg ccgtcattac
     7081 cctcgctggt gacttccgtc ggccctatgc tcggtgggta acgtggacgt ctattcgtgc
     7141 tctcggtggc gcgtctgctg cattggccgc gtcggcagtt gctgtgagcg acccaacgtt
     7201 tgcgaacctc tttgctgctg tcgcggcggc cgccgtcgct gaggcgctcg tagatgtagc
     7261 cctcggtgcc gtgaccggtg cccttcgagg catcgcctcc gtaagcgcaa cgcttcaact
     7321 ggtaaaacca gtgctcctag gcacggttcc cctctacgca ccggtcgttg tcctactgat
     7381 ttatgcctgc cgcgagattt ccgaatggag cgttgtcttc ttcgtcgtgc cggccttcgc
     7441 tgcccaccgg ttctactcgc tgtatcgaga ggagcatgat gctcgccgag agctcaacga
     7501 ggttaacgcc cgtctccagc gggcaaacct ttcgttcgca accgcgctcg tagcaactct
     7561 tgatgcgcgg gaccgctaca ccgcgggtca ttcggccgca gttgcggtat acgcgcgaga
     7621 catcgcaagg cgcctcgggc tcagcgaaga agagcagcga ctcgcccacc tctgcggtct
     7681 cgttcacgac atagggaaga ttggcctgcc gccgggcctc ctcgaaaagc ccggcgccct
     7741 cacgctcgac gaacgccgtc agatggagca acattccgag atcggcgagc ggattctcgc
     7801 gaatgtcgag gactacagcg agatcgcaag aatcgttcgt catcatcatg agcgaatgga
     7861 tggagaggga taccctgacc gaatcgctgg ttctgatatt ccgctgttgt cgagaattat
     7921 tgcggttgcg gacgcttaca acgcaatgac ttccgaccgc ccttaccggg acgcgatgcc
     7981 gagtcgtgtt gctcggctgc ggctagcaca ggcagtggag acgcagttcg acacgaacgt
     8041 tgtagctgct ttcgaggcaa ttctcgccag tgcgggtgaa gattatcgcg ccgggcttaa
     8101 ccttctcttt gatcgcgagg cccaggccca cgaagagctt ggacgtccgc tgatcgcgtc
     8161 ggtcgcctag ggaggaaccg ccgacagcat taccgccgct tcagcgcgag tggtagcacc
     8221 aagcttctct agggcgtgcc taacgtgcac ctttacggta gcctggctaa tgacgaggtg
     8281 cgcggcgatc tgcttgttcg taaggccctt tgccacaagg ccgagcacct ctcgctcgcg
     8341 cggagtcagt cgtccgagcg gtccgatcag tggcgccgta acttcgacgc cgattctgcg
     8401 ggcgagtttg acatcgttcg cccgggcgac gattgaccga gcgacgtcac gaaaggacgc
     8461 ggaagcgaac gcagcggaaa gaaggacggg gaacgttcga taggcggcta cgaactcatc
     8521 gaatcgtcct ccgtccagta caaaagcgag ggattctgcc accgcctcgc gtgcacctgg
     8581 attagactcc aggacggaga ctgccttcac gagcgtggcc gtcgtggcgc ccccgcgcgc
     8641 gaaccgcggt gcaagcagca cctgattgga gagctccgtc gcagttccga agtcgccggt
     8701 tgcggcggcg gcgagtgctc gactactcaa gtactcggcg tacatcgcgt ctgaaggcag
     8761 acgaccaagt ggtgtgctca aggttgcaaa ggcttggtcg ggatgtcctc gcaatagtgc
     8821 tagtcgagct cggagagtcg ctgcgcctgc ggtgatatgc gggtcttctg ggccttgctc
     8881 ggcgtgagct attgcatcct ctgcggcaga cacatcacga aggccaatgg cggccattcc
     8941 tcggaggatc gcgacgtgag aaagaacgaa gtcgaggcga tactctctcg cgaccgtttc
     9001 agcctcgtcc gcgagccgta ttgccgtctc ataccaagcc atgagaccta gcgactgccc
     9061 ataggaattc aagaacgaag tcgtgatgac cggatccgtg actctgtcta gaagtggata
     9121 tacgtctgcg gcggagttca gagcgtccgc caatcctccc gttcgctccg cataaagcat
     9181 tcgagcgatg ctcagacgga ccagatcctc agatcctcgc gttgagatgg cttcgaggtc
     9241 atcaagaagt tcggcaacct catccaagcc gagatcgacg cacgaaaaga actggccaat
     9301 agccgcctcc cgctcgagcg aacccgttgg cgcgatcttg cgggcttcgc ggaaatggcg
     9361 caaagcctca gtttcacggc tatctaagag cgcgctctgt cctgctctta gaagtatgag
     9421 agcgcgcagt cgctgcccgt actccggtgc tccgtcagca attcgtgctg cgttcgacgc
     9481 gagtgcggca gccgtgccat aacgaccgtg gcgaaacgct agctcggcct cagcgaaatc
     9541 gaggatcggg gcagttaagt ggtgcttcgc ggcgaactcc aaccattgag ccagcgttgt
     9601 cactctcccg gtttcgatga tgtcctcaac cgcggccgac acgagtgctt caagccggtc
     9661 gtgtacggaa aagcgatcta ttacgtcgaa cgcgtcatcc cacgcaccag ttcgggtgag
     9721 gtgatcaacg acttgtccag caagctcaaa actattcgcc tcctccagtg agactagttt
     9781 cgcctcaagg aaggtacgga tcagtgggtg catctcatac gttgcacctt gagtctggag
     9841 gattccaagt cgacatccga cttcgagtgc gggcgcgtaa gactctccga acaggaagcg
     9901 cgccatgtct ggagtgatca cccgagtgag gcagagcttg cacagcgcaa gccgaagcgg
     9961 cggggcgact tgctggtaga gctcttccgc gaagtagtcg tacagtgcgg gagggagtcc
    10021 gtgctccggc gctctgaggt ggtccgtcgt cgccgcgagg cctatcacgg cgggccagcc
    10081 gcgggcatgc gccaataggg cgatgacatc ctgatcgtcg cgtcctatga cgctctttgc
    10141 ttcctccgcc gccattgcga gctctcgtcg atcaatttcc tggagctctc cgtacacaat
    10201 ccgccttgca gtggcccagg tgggacgtcg ccggctcgtc acgatcagcc ggataggcgt
    10261 gtcttgcact agtaactcga gaaatcgctc cgaggccgtt gactccatcg cgaactgata
    10321 gtcgtcaatc gcgagccaga cgtctgatgg ccactgctga atgtcttcgg caaagagagc
    10381 agctaggaga tcgacgtgat cctccggctg gccgaccgat cgaatgcgct ttcgcattcg
    10441 gccgccggca tcggggatca aatcgccaat gaggttcgaa acgccagccg cgagcgcggc
    10501 cacatccgct gaggcgggac ctccgcgata ccacagacct tggcgaggtg gtgtgctgag
    10561 ccattgctgt gccagcgtcg tcttgccgta gccggccggg gccacgagca gcttgatccg
    10621 cgccgggctc tcgtccagga gcttcgtcag gcgcgggcgg gggatgatcc gccggcgagt
    10681 tgcaacggcc tctgctgcca tgacgccagc aagaggctaa acgccgggcg gcgaaatctc
    10741 gactcgtcga aaccggggtc cgcgcctgcg ccgcggtcgc aggcgcggtg ccgtcagtcc
    10801 cccgagccga gggttaccgg cccgaggctt cgagaaagcg gatccttgcc ggtgagcagc
    10861 cggtaggcga ccggaatctg ccgccgccgg gctacgccaa gcttctgccg caacacgtcg
    10921 caatgcgcct tggcggttcg cggcgagata cccagtcgct cgccgacctc ctcgttcgag
    10981 catcccgcgg cgatcagctc gatcacctgc agctggcgct gcgtgatgct cggcgggatt
    11041 ctcgtgagtg cctcctgcat tcgggcgacg ctaacaacgc ccgctccggc tggctattga
    11101 ccgaacggcg tatttcgtgg actacgccct gggatagttc agcgaaacgc ggctgtgccc
    11161 gccgggcgac gagcaccgct ccacgaaccc ctcctacgca cccgtaggca ccgggccggc
    11221 cgcccgatgg acgccggaca gcgccggagc ccacgagcac agcggcgaca cgtcgccgtc
    11281 gcgcccgttc agcgcgcgcc agccgagtgc gtgttcggcc tgcatcatct tgtgcacctg
    11341 cgtggtgccc tcgtagatga tcggcgcccg cgcgttccgg aagtaccgct cgatgccgta
    11401 ctccgccgag tagccgtacg cgccgtgcac ctggatggcg aggtgcgcgg ccttgaacgc
    11461 cgactccgtc gcgtgccact tcgcgagtga cgtctcgcgc gtgttgcgcc ggccctcgtt
    11521 cttcatccac gccgcctgca tcacgagcag cttcgacgtc tcgtagccga gcaccatctc
    11581 ggcgatcatg tcctggacga actggtactt gccgatcggc tgcccgaacg tctcccgctc
    11641 gcgcgcgtac tccaccgacc gctcgaggca cgcccggatc acgccgcacg cgcccgcggc
    11701 gaccgtgaag cggccctggt cgagcccgta catggcgatc tcgaacccct ggccctcctc
    11761 gccgacgagg ttctcggccg gcacctcgac gttctcgaag aagagctcgc cggtcgaccc
    11821 cgcccagatg ccgagcttgt gctcggtgtc ccgggtcgac acgcccttcc agccacgctc
    11881 gacgacgaag gcggagatgc ccttgtgctt cgccgacggg tcggtcttcg cgaagacgag
    11941 ctggtggtcg gcgaccgacg cgtacgagat ccagttcttc tggccgttga gaacgtaggt
    12001 gtcgccctcg cgccgggccg tcgtccgcat cgacgccacg tccgacccgg ccgccggctc
    12061 cgtcaggccg aagcacgcga gcttctcgcc ccgcgcctgc gggacgaggt agcgctgctt
    12121 ctgctcctcc gtggcgtacc ggagcagcga gagcgagttc agcccgacgt ggacggagat
    12181 gagcgtccgg aaggccgcct cgccgcgctc gatctcctcg caggagagcg cctcggcgac
    12241 gaagtcgagg ccggcgccgc cgtactcctc ggggatcacg atcccgagca ggccgagctc
    12301 ggccatgcct tcgatcacgc tcatgtcgag ccggtgctcg atgtcgttct caacggcgtt
    12361 cgggaggacc ttccggtcga cgaaatcgcg gacggtgtcc tggatcagcc gctgctcgtc
    12421 ggtgagctcg aagtccatgc cggcgaggag tgtacgggtc gcggcctggc tacaatcaga
    12481 cttgactgac tcgtcaatca atcccaggat ggcgactatg acggcgatcg aggaagcaat
    12541 ggccacggcg accggcgtct ctttcgcgct cagcgacgag cagcgggaat tgcgccggct
    12601 cgcgcgggag ttcgcggaga gggagatccg ccccaaggcc gccgagtacg acgagcacca
    12661 gacgcacccc gccgacgtcg tcgcgaaggc gcacgaggtc gggctgatga acccgcacat
    12721 cccggaggcg tacggcgggc tcggcctgcc cggcttcgaa gggatgctga tcggcgagga
    12781 gctctcgtgg ggctgctcgg ggatcgcggt ctcgatcgtc gccaacacgc tcggcgccgc
    12841 gcctgtcgtg ctggccggga gcgaggagca gaagatcaaa tggctgccgc cgctcgtcga
    12901 ggagccgatc ctctgctcgt tcgggctcac ggagccgaac gcaggctcgg acgtctccgg
    12961 gatccagacg accgcagtcc gccagggcga cgagtacgtc atcaacggtt cgaagatgtt
    13021 catcacgaac gccggccacg cggactggct cgtcgtgttc gcctccacgg acaagagcaa
    13081 gggccaccgc gggctctcgg cgttcgtcgt cccgaccgag ctcgacggcg tcgtcgtcga
    13141 gaagcacctg gacaagatgg gtcagcgcgc gactgacacg tctgcgatcg cgttcagcga
    13201 cgtcaaggtg ccggtcgaga accggctcgg cgaggaaggc gagggcttca agatcgccat
    13261 gcggacgctc gacttcaccc gcccgggcac agcagccggc gccgtcgggg tcgcccaggc
    13321 cgcgttcgac tactcggtcg agtacgcgaa gacgcgggtg cagttcggac agccgatcgc
    13381 gatgaaccag ggaatcaact tcctggtcgc ggacatggcg accgagatcg aggcggctcg
    13441 gctcctcgtc tggcaggcgg cgtggctgct cgaccagggg aagcgcgcga cgctgcagtc
    13501 gtcctttgcg aagcggttcg cggccgacac ggccatgaag gtgacgacgg acgccgtcca
    13561 gatcttcggc ggctacggct acatcaagga gtacccggtc gagaagctca tgcgcgacgc
    13621 gaagctgttc cagatctacg agggcacgtc gcagatccag cggctcgtga tcgctcggga
    13681 gatatttctc ccgcgtgacg attgagatcg acctcagcgg gcgggtagcg ctcgtcaccg
    13741 gcggatcgcg cgggctcggc cgtgccgacg cgctgacgct cgcgcgcgcc ggggccgacg
    13801 tcgtgatcgc ggacatcctc gtcgagtcgg acgactcggc cgcggaggtg tacgggccgc
    13861 tggcgcaggc ggccgcggca cgtgggctcg tccagaccga ggcgaccgcg gaggagatca
    13921 ggggcctggg tcgccgctcg ctcgcgctca agtgcgacgt gacggactcg gatcaggtcg
    13981 aggcggccgt cgcgcgcacg gtcgagggcc tcggctcggt cgacatcctg gtcaacaacg
    14041 ccggcacgct cgaccacctc ggccagctcg agcaccaggt cccggagctt tgggagcgcg
    14101 acctgcgtgt caacctcacc ggcgccttca actgctcgcg cgcggtctgg ccgcacatga
    14161 aggagcgcgg ctggggccgg atcgtgaaca tggcgtcggt ggcagggacg ctcgggggct
    14221 tcggccaggc gagctactcg acgacgaagg cgggcctcat cggcctgacg aagacgctcg
    14281 ctttggaggg cgcccgccac gggatcaccg cgaacgcgat cgtcccgggc atcatcggga
    14341 cggaggcttt ccatttcggc aatcccgaca tgaacgagcg gatggtcaaa cgcaccgcgc
    14401 tccggcggac cggcgacccg caggacatcg cgaacgcgat cgccttcctc tgctc
//
LOCUS       DASVEU010000193         8084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1102676,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000193 DASVEU010000000
VERSION     DASVEU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102676"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..926)
                     /locus_tag="VF225_07935"
     CDS             complement(117..926)
                     /locus_tag="VF225_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145226.1"
                     /translation="MPRLSVNGTELYHEVRGTGPPVLLIMGASGDGGHFDVLADLLAD
                     EFMVVTYDRRGNGRSEVPAGWETTSAEEQADDAAALVTALGTGPAAVFGTSSGGSFAL
                     CLLARHPAAVRGAILHEAPLFALVDDLDAVRAPLLARVRPAMETGGAPAATEPWWRYV
                     AGDDSWTKLEPALRERMRASAHTLFEIELGTYERYLPDDETLAAIAPRVRLLISTEGL
                     PAYAEVARQLGERLGVNVHYTPGTHAAYHDHPHELAERIRPLLRELSAISP"
     gene            complement(1194..2408)
                     /locus_tag="VF225_07940"
     CDS             complement(1194..2408)
                     /locus_tag="VF225_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7145227.1"
                     /translation="MATYAFKALDLAGMPSKGEMDAGDKQAVAAQLRGRGLIVIDIEE
                     QTPASAGDLLARFKRVKADELVIATRQLATMVNSGMSLLRSLYVIEEQTENDKLREIF
                     VDVRKDVEAGLALSDALRKHPDMFNELYVAMVQAGETGGILDNTLMRVADQLEKDAAL
                     RRQIKAAMMYPALIGGFAFVVLFALVAFLVPVFEKIFKDFGGELPAITKFTVFLSHLV
                     TQRWYLLFGGTFAIVWTFKKWKKSERGQMQWDRIKLKFPMKIGGIVQKVALARFSRTF
                     SGLIAAGVPMLEAIDITGKTSGNKVVEKAMQQVRESVKKGGSLTQPMTQVPEAFPVMV
                     TQMIGVGEETGALETMMTKVADFYEEQVEAAVKALTSILEPIMILFVGGIVGFIVIAM
                     YLPMFKVYDSIK"
     gene            complement(2408..3481)
                     /locus_tag="VF225_07945"
     CDS             complement(2408..3481)
                     /locus_tag="VF225_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7145228.1"
                     /translation="MEFDFAEVLLQVMERNASDLHLTAGSPPMIRHHGRLHPLDYPNL
                     TPQLTREVIYSILTNDQRQRLETDWQIDFAYSIPGKARFRVNAYMQRAALSAAFRLIP
                     HDMPKLSTLGLPPGLEDFTKKPRGFVLVTGPTGSGKSTTLAAMLDLINDTRHEHILTI
                     EDPIEFLHKHKRCIVNQRELGADAQSFALGLKAALRQDPDVILVGEMRDLETISTALT
                     AAETGHLVFATLHTQDTAQTVDRIVDVFPPAQQQQVRVQLSVSLQGIVTQQLLPTADG
                     KGRCVAVEMLVPTPAVRNLIREGKTHQIYSTLQTGGAHGMQTMDASLVQLVREGKISR
                     ELAEARSSSPDELRRLMGTVRAA"
     gene            complement(3510..5276)
                     /locus_tag="VF225_07950"
     CDS             complement(3510..5276)
                     /locus_tag="VF225_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7145229.1"
                     /translation="MSKKEGAAAPLRAVPEGEAEAPLEPTTNGLTPPTKKAGSSRFIS
                     DVIVEMGFLPRERVDVAVDEGKASGRSPEQVLLETGAITGDQLARAVAQRFGLHHIDL
                     SIYKTDVGALNSITPQAARRLGAVPVGVDENGLLLVAMSDPSNVLALDDLKLMTGNEV
                     VPLVAAPDDIVGLIGRMSRYDDAVAAAIDSGEEELAEVSDIRESADDAPVIKLVNSII
                     GQAVEEGASDIHFEPEGREMRVRFRVDGILRETTQIPRRMIAGVISRVKIMADLDIAE
                     KRVPQDGRVSLTVEKHAIDTRVVTMPRVDGEGIVMRILDKSQALIGLEQLGMAEVSEE
                     LFEDAFRRSYGAVLVTGPTGSGKSTTLYAALNEINEPQRNIITIEDPVEYQLPGVNQL
                     QVNLKAGLTFAGGLRAMLRADPDVIMVGEIRDGETAKIAVESALTGHLVLSTLHTNDA
                     PSAITRLTEMNIEPFLSASAIVCVVAQRLVRQLCTYCKSRTMLSVSSLEAAGFNVAFD
                     LEAYEPIGCARCGGTGYKGRTGLYEVMSMSDEIRDLTIERSSSDEIRKVAVAQGMRPL
                     RADGFEKVKNGVTSIAEVARVT"
     gene            complement(5273..6565)
                     /locus_tag="VF225_07955"
     CDS             complement(5273..6565)
                     /locus_tag="VF225_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145230.1"
                     /translation="MALSRPFLLALLGVALLGATFFAVTNARDNAADDSAPVAQQTAD
                     PAATPAQATPEPAAAPAAPQELLAAGLTPDALESASFDGALSFSSNGEKNTIKTSGAF
                     ELGGAKEMPQIDVQVNVDVPGFDGRAGFVTTGKRAWFTRGDTGYAVPQKAWTRIVAAR
                     ESGAAPAADTPQVDVDPSSWLRNVKSEGTEKMDGVDVTHVSADVNSAKAITDVVKAMD
                     RTGQVPAGAEARLDGVVKNGHIDAWVGDDKIMRRVSLELSGKGDGGRAVSMDLDFKLS
                     GVNKPQDISRPAKVKYALPSGVYGQVASGFVTGLGSSVGVQADDLQLNVPITNSHVKA
                     ERAVADNRKVVIFFQNPKGIDDKAVAKSVRSLDRRTKSVVVLTDDVRNADRYGSMVED
                     LGVNQAPAIVVIGRSGKASLIEGYIDAESLVQVVADAR"
     gene            complement(6618..7394)
                     /gene="aroE"
                     /locus_tag="VF225_07960"
     CDS             complement(6618..7394)
                     /gene="aroE"
                     /locus_tag="VF225_07960"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7145231.1"
                     /translation="MSTLTGVLGFPVEHSRSPLMMNAAFAELGIDWRYLKLPVPPQRF
                     DETVRALPASGYRGANVTIPHKLAAHDLADELSDAARAIGAVNTLTFRDDGRIAGDNT
                     DAGGLLDALGQSLPPSALVLGAGGAGRAAAWALAQVGVEVTVWNRTPERAVELAAALG
                     VSASERPGPAELLVNATSVGLRPADSLDGLPLVDARVVVDLVYGAAQTPFARWAEERG
                     ARLVDGLEVLVRQGARSLELWTGEAPPVDSMRRAVVQPVV"
     gene            complement(7391..>8084)
                     /gene="mltG"
                     /locus_tag="VF225_07965"
     CDS             complement(7391..>8084)
                     /gene="mltG"
                     /locus_tag="VF225_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7145232.1"
                     /translation="EGLSRKEIAPLTGGLRGSYVRATRRAPSLNPRDYGAKGATSLEG
                     FLFPATYELKKGQTVARLRDQQLAQFKRNFDKVDLSYAKRKNLTAYDVLIIASLVERE
                     AMLAKERPIIASVIYNRLHDDIRLDIDATTRFAVGNWKRPLRESELQNQSPYNTRVHP
                     GLPPGPIGNPGLASIKAAAHPAKTGFLFYVVKPGTCGQHNFAKTDAEFQGYVNEYNRA
                     RDENGGKSPTTC"
BASE COUNT         1256 a         2709 c         2719 g         1400 t
ORIGIN      
        1 gcaactctcc tcggacgatc cgccgtgctc tgctggttac gcgaaaattg cgaggtcagc
       61 gagccgcgcc actcctgctt ttcgtccatt gcgatagcgc ggcctcatga gcactgctac
      121 gggctgatag cgctgagctc ccgcaggagc ggccgaattc tttcagcgag ctcatgtgga
      181 tgatcgtgat aggccgcgtg cgtaccgggt gtgtagtgga cgttcacgcc tagccgctcg
      241 ccgagctggc gggcgacctc cgcgtaagcc ggaaggccct cagtgctgat gagcaaccgc
      301 acgcgcggcg cgattgctgc gagcgtttca tcgtccggca gataccgctc gtacgttccg
      361 agctcaatct cgaacaacgt atgcgcactc gctcgcatcc gctcgcgcag agcaggctcg
      421 agcttcgtcc agctgtcgtc tccggccacg tagcgccacc agggctctgt cgccgcgggc
      481 gccccgccgg tctccatggc cggccggacg cgcgccagga gcggcgctcg cacggcgtcc
      541 aggtcgtcaa ccaaggcgaa caaaggcgcc tcgtgcagga tcgcgccccg taccgcggcg
      601 ggatgacggg cgagtagaca caacgcgaag ctcccgccgc tgctcgtgcc gaagaccgcc
      661 gccggaccgg tccccagcgc agtcacgagc gccgcggcgt catccgcctg ttcctccgcg
      721 gacgtcgttt cccacccagc cgggacttcg ctgcgcccgt tgccgcgtcg atcgtaagtc
      781 acgaccatga actcgtccgc gagcaggtca gctagcacgt cgaaatgccc accgtcgccg
      841 ctcgctccca tgatcagtag gacagggggt cctgtaccgc gaacctcgtg gtagagctcg
      901 gtcccgttaa cgctcagtcg cggcatgagg cctggcgagt tcctcagcaa agactctcgc
      961 gtcaggtcgc ccagcgcaaa cctcaacgaa cggcgctgct gctttccatg caaaacagca
     1021 gtgccagctc atcgattgtg cgctaccgct tcgcgcgaaa agcgacagcg gcagtttcgt
     1081 tagtgctgcg ccgcacctgc gcgcctgagg agacgggcct ctcgcgacct acggaaccga
     1141 aggtctcagg ttccagtcct ggcgggcgct ccccgaattc cccgccgcgt gcgctacttg
     1201 atcgagtcgt agaccttgaa catcggcagg tacatcgcga tcacgatgaa tccgacgatg
     1261 ccgcctacga agaggatcat gatcggctcg aggatcgagg tcagggcctt gacggcggcc
     1321 tccacctgct cctcatagaa gtccgcgacc ttggtcatca tggtctcgag ggcaccggtc
     1381 tcctcgccca cgccgatcat ctgggtgacc atgaccggga aggcctcggg cacctgcgtc
     1441 atcggctggg tcagcgagcc gcccttcttg accgactcgc ggacctgctg catcgccttc
     1501 tcgacgacct tgttgccgga cgtcttgccg gtgatgtcga tcgcctcgag catcggcacg
     1561 ccggccgcga tgaggccgga gaaggtccgc gagaagcgcg ccagtgcgac cttctgcacg
     1621 atgcccccga tcttcatcgg gaacttcagc ttgatccggt cccactgcat ctgcccgcgc
     1681 tcggacttct tccacttctt gaaggtccac acgatggcga acgtgccacc gaacagcagg
     1741 taccagcgct gagtgaccaa gtgtgagagg aagacggtga acttcgtgat ggcgggaagc
     1801 tcgccgccga agtccttgaa gatcttctcg aagacgggca caaggaacgc cacgagcgcg
     1861 aacagcacca cgaaggcgaa cccgccgatg agcgccggat acatcatcgc ggccttgatc
     1921 tggcggcgca gcgcagcgtc cttctcgagc tggtccgcaa cgcgcatcag cgtgttgtcc
     1981 aggattcctc cggtctcacc ggcctggacc atcgccacgt agagctcgtt gaacatgtcg
     2041 gggtgcttgc gcagcgcgtc cgacagcgcg aggccggcct ccacgtcctt gcggacgtcg
     2101 acgaagatct cgcgcagctt gtcgttctcg gtctgctcct cgatcacgta cagcgagcgg
     2161 agcagcgaca ttcccgagtt caccatcgtg gcgagctgcc gcgtggcgat cacgagctcg
     2221 tccgccttca cgcgcttgaa gcgcgccagg aggtcgcccg cgctcgcggg agtctgctcc
     2281 tcgatgtcga tgacgatcag gcccctgccg cgcagctgcg ccgcgacggc ctgcttgtcg
     2341 ccggcatcca tctcgccctt cgacggcatt cccgccaggt cgagtgcctt gaatgcgtag
     2401 gtggccatca ggcagccctc acagtgccca tcaggcgacg gagctcgtca ggcgacgagg
     2461 agcgggcctc ggccagctca cgcgagatct tgccctcgcg cacgagctgg acgagcgagg
     2521 catccatcgt ctgcatgccg tgtgcgccgc ccgtctggag agtggaatag atctggtgtg
     2581 tcttgccctc gcggatcagg ttgcggaccg cgggggtcgg cacgagcatc tcgacggcca
     2641 cgcatcgtcc cttcccgtcg gcggtcggca ggagctgctg ggtgacgatg ccctggaggg
     2701 acaccgacag ctgcacgcga acctgctgct gctgagcggg agggaacacg tccacgatgc
     2761 ggtccaccgt ctgcgcggtg tcctgcgtgt ggagcgtggc gaacacgagg tgaccggtct
     2821 ccgccgcggt cagcgcggtc gagatcgtct cgaggtctct catctcgccg acgaggatca
     2881 cgtcggggtc ctggcggagc gcggccttca gcccgagggc gaagctctgg gcatcggcgc
     2941 ccagctcgcg ctggttcacg atgcaccgct tgtgcttgtg caggaactcg atcggatcct
     3001 cgatggtgag gatgtgctcg tggcgggtgt cgttgatcag gtcgagcatg gccgcgagcg
     3061 tcgtggactt gcccgaaccc gtcggaccgg tcacaaggac gaagccacgc ggcttcttcg
     3121 tgaagtcctc tagccctggc ggaaggccga gcgtggagag cttcggcatg tcgtgcggga
     3181 tcagccggaa ggcagccgag agcgccgcgc gctgcatgta tgcgttcacg cggaagcggg
     3241 ccttacccgg aatcgagtag gcgaagtcga tctgccaatc ggtctccagc cgctgacgct
     3301 ggtcgttcgt gaggatcgag taaatgacct cacgagtgag ctgcggcgtc agatttgggt
     3361 aatccaacgg atgcagccgt ccgtggtggc ggatcatggg cggtgagcct gctgtgagat
     3421 gaagatccga agcattgcgc tccatcacct gcagcaggac ttctgcgaag tcgaactcca
     3481 tcaggactcc tatcggccgg agggtgcaat cacgtgactc ttgccacctc ggcaatggac
     3541 gtgacgccgt ttttgacctt ttcgaagccg tcggcgcgca acgggcgcat gccctgcgcg
     3601 acggcaacct tgcgaatctc gtccgacgag gagcgctcga tcgtgagatc ccgaatctcg
     3661 tcgctcatcg acatgacctc gtagaggccc gtccggccct tgtagccggt cccgccgcag
     3721 cgagcgcagc cgatcggctc gtaggcctcg aggtcgaacg cgacgttgaa gcccgccgcc
     3781 tcgagcgaag acacgctcag catggtgcgg ctcttgcagt acgtgcagag ctggcgcacc
     3841 agccgctggg caaccacgca gacgatcgcg gacgcgctca ggaagggctc gatgttcatc
     3901 tcggtgaggc gggtgatcgc cgaaggcgcg tcgttcgtgt gaagcgtcga cagcaccagg
     3961 tggcctgtca gggccgactc gaccgcgatc ttggcggtct cgccgtcccg gatctcgccg
     4021 accatgatca cgtcggggtc ggcgcgcagc atggcccgca ggccgccggc gaacgtgagg
     4081 cccgccttga ggttcacctg aagctggttg acgcccggga gctggtactc gaccgggtcc
     4141 tcgatggtga tgatgttccg ctgcggctcg ttgatctcgt tgagcgcggc gtagagcgtc
     4201 gtcgacttgc cggagccggt cgggcccgtg acgagcaccg cgccgtacga gcgccggaat
     4261 gcgtcctcga agagctcctc gctgacctct gccattccca gctgctcgag cccgatgagg
     4321 gcctgagact tgtccaggat tcgcatcacg atgccctcgc cgtccacgcg cggcatcgtc
     4381 acgacacggg tgtcgatcgc gtgcttctcg acggtgagcg acacccgtcc gtcctggggt
     4441 acgcgcttct cggcgatgtc gaggtccgcc atgatcttca cgcgcgagat cacgccggcg
     4501 atcatgcgcc gcgggatctg ggtcgtctcg cggaggatgc cgtccacgcg gaagcgcacg
     4561 cgcatctcgc gcccctcggg ctcgaagtgg atgtccgacg ctccctcctc gaccgcctgg
     4621 ccgatgatgg agttcacgag cttgatgacg ggcgcgtcgt ccgcggactc gcggatgtcg
     4681 ctgacctcgg ccagctcctc ctcgcccgag tcgattgcgg ccgcgaccgc gtcgtcgtag
     4741 cggctcatcc ggccgatcag cccgacgatg tcgtcgggcg ccgccaccag cggcacgacc
     4801 tcgttgccgg tcatcagctt gaggtcgtcg agagcgagga cgttggacgg gtcggacatc
     4861 gccacgagca gcagaccgtt ctcgtccacg ccgaccggca ctgcgccgag gcggcgcgcg
     4921 gcctgcggcg tgatcgagtt gagcgcgccc acgtccgtct tgtagatcga caggtcgatg
     4981 tggtgcaggc cgaagcgctg cgccaccgcg cgtgcaagct ggtcgccggt gatcgcgccg
     5041 gtctcgagca gcacctgctc gggcgagcgg ccagaggcct tgccctcgtc caccgccacg
     5101 tccacgcgct cgcgcgggag gaagcccatc tcgacgatca cgtcggagat gaagcgcgac
     5161 gatccggcct tcttcgtggg aggagtgagc ccgttggtgg tgggttcgag cggggcttcg
     5221 gcctcgccct caggcacggc acgcaacggc gccgccgcac cctccttctt gctcatcgcg
     5281 cgtcggcgac tacctggaca agcgactcgg cgtcgatgta gccctcgatc aggctcgcct
     5341 tgccggagcg cccaatcacc acgatcgccg gtgcctggtt gacgccgaga tcctcgacca
     5401 tggacccgta acggtccgcg ttccgcacgt cgtcggtgag cacgaccacc gacttggtcc
     5461 gcctatcgag cgaccggacc gactttgcga cggccttgtc gtcgatgccc ttggggttct
     5521 ggaagaagat cacgacctta cggttatcgg cgacagcacg ctcggccttc acgtgggagt
     5581 tcgtgatcgg cacgttaagt tgcagatcgt cggcctgaac gcccacgctg ctgccgagac
     5641 cggtcacgaa gccgctggcg acctggccgt agacaccgct cggcagggcg tacttgacct
     5701 tggccgggcg gctgatgtcc tgcggcttgt tcacgccgga cagcttgaag tcgaggtcca
     5761 tcgagacggc acggccgccg tcgcccttgc ccgaaagctc gagcgagacg cggcgcatga
     5821 tcttgtcgtc gccaacccat gcgtcgatat ggccgttctt gaccacgccg tcgaggcggg
     5881 cctcggcgcc ggcgggcacc tggcccgtgc ggtccatggc cttgacgacg tcggtgatcg
     5941 ccttcgcgga gttcacgtcg gcggacacgt gcgtcacgtc gacgccgtcc attttctcgg
     6001 tgccctcgga cttgacgttg cggagccagc tgctcgggtc gacgtcgacc tgcggcgtgt
     6061 ctgccgccgg cgctgcgccg ctctcgcggg cggccacgat cctcgtccac gccttctgcg
     6121 ggacggcgta gcccgtgtca ccgcgcgtga accaggcacg cttgccggtt gtcacgaagc
     6181 cggcgcgacc gtcgaagccg ggcacgtcga cgttcacctg aacgtcgatc tggggcatct
     6241 ccttggcgcc gccgagctcg aaggcgccgg aagtcttgat ggtgttcttc tcaccgttgg
     6301 agctgaacga cagggcgccg tcgaagctcg cgctctcgag cgcgtcggga gtgaggccgg
     6361 ccgccagaag ctcctgcgga gcggcgggcg ctgctgcggg ctcgggcgtc gcctgagccg
     6421 gcgtcgcggc cggatcagcc gtctgctgcg ctaccggcgc cgagtcatcg gcggcattgt
     6481 cgcgagcgtt tgtaaccgcg aagaacgtcg ctccgaggag cgcgaccccg aggagggcga
     6541 ggaggaaggg gcgggagagc gccattgcct cgcatatcgg caggcgtccg cttcatgttt
     6601 agacctgggc gtatgtacta gacgacgggc tggacaaccg ctcgccgcat ggaatccacg
     6661 gggggcgcct caccggtcca gagctcgagg cttcgagccc cctggcgcac gagcacctcg
     6721 aggccgtcca cgaggcgcgc gccgcgttcc tcggcccagc gcgcgaaggg cgtctgtgcg
     6781 gctccgtaga cgaggtcgac gaccactcgg gcgtccacca gcggcagccc gtcgagcgag
     6841 tccgcgggac ggagcccaac tgaggtggcg ttcacgagca gctccgcagg cccgggacgc
     6901 tccgaggcgg acaccccgag cgctgccgcc agctcgacgg cccgctccgg cgtgcggttc
     6961 cagacggtca cctcaacccc gacctgcgcg agcgcccacg ccgccgctcg cccggcgcca
     7021 cccgctccga gcacgagcgc gctcgggggc agcgactggc cgagcgcatc gaggaggccg
     7081 cccgcgtcgg tgttgtcgcc cgcgatccgc ccgtcatcgc ggaacgtgag cgtgttcacg
     7141 gcgccgatcg cgcgcgcggc gtcgctcagc tcgtcggcca ggtcgtgcgc cgcgagcttg
     7201 tgcgggatcg tgacgttcgc cccgcggtag ccggacgccg gcagggctct caccgtctcg
     7261 tcgaagcgct gcggcggcac gggcagcttg aggtagcgcc aatcgatgcc gagctccgcg
     7321 aaggcggcgt tcatcatcag cggagagcgg ctgtgctcga cggggaagcc gaggacgccg
     7381 gtgagcgtgc tcaacaggtg gtcggcgact tgccgccgtt ctcgtcccgg gcgcggttgt
     7441 actcgttcac gtagccctgg aactcggcgt cggtcttggc gaagttgtgc tgaccgcagg
     7501 tgcccggctt gacgacgtag aacaggaagc cggtcttggc cggatgcgcc gcggccttga
     7561 tcgacgccag acccgggttg ccgatcggac ctggtggcag accgggatgc acgcgcgtgt
     7621 tgtacgggct ctggttctgg agctcggact ccctgagtgg gcgcttccag ttgccgacgg
     7681 caaagcgcgt ggtcgcatcg atgtcgagcc ggatgtcgtc gtggagccgg ttgtagatca
     7741 ccgaggcgat gatcggccgc tccttggcga gcatcgcctc gcgctccacc agcgacgcga
     7801 tgatcagtac gtcataggcg gtgaggttct tgcgcttcgc gtagctcagg tcgaccttgt
     7861 cgaagttgcg cttgaactgg gcgagctgct ggtcgcgcag gcgcgccacc gtctgcccct
     7921 tcttcagctc gtaggtggcc gggaagagga agccctcgag gctggtggcg ccctttgcgc
     7981 cgtagtcgcg cgggttgagc gagggcgcgc gccgagtggc gcgcacgtag ctcccgcgca
     8041 ggccgccagt gaggggcgcg atctccttgc gcgaaaggcc ctcg
//
LOCUS       DASVEU010000194        15341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1491920,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000194 DASVEU010000000
VERSION     DASVEU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491920"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF225_07970"
     CDS             complement(<1..321)
                     /locus_tag="VF225_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145233.1"
                     /translation="MASLEERTRERLPPEIFDLPVEKMREGYYADAYFNHARATLLQD
                     ARRPRVVMQVFQKNDAYLGGMDEAIAILKLCSYDWDELTVHALYDGDRVAPSETVMTI
                     EGDYT"
     gene            complement(324..1100)
                     /locus_tag="VF225_07975"
     CDS             complement(324..1100)
                     /locus_tag="VF225_07975"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7145234.1"
                     /translation="MSDEKIRLGGMALANGVLVHGPRSWACAIRTEDGRLKVAAQRKR
                     LRASKVESPLLRGPVRLLDAMAIIPEIKRALPEAKLPFQRPVVVAAMVGSATVLRLLR
                     GSRSVGPAVQEVVSGLLSVAPAALALRGSDVAAYHGAEHISIGSYEQGKRAAKEHNRC
                     GSHLIGPLLATTAIGNVLAARAPAHLRRPARLGAQLVALAASTEIFGWMVRNPENRLA
                     RALAKPGHELQHRLATAEPTPEQIEVAEAALAACLELEQE"
     gene            complement(1152..2453)
                     /locus_tag="VF225_07980"
     CDS             complement(1152..2453)
                     /locus_tag="VF225_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7145235.1"
                     /translation="MATVSARDEVTELLQELIRVDTTNPPGNETAAAELLRSYLEASG
                     AQVELYARIPERANLVARIPGRGDGPTLLFLSHTDVVLADASEWSADPFGGELRDGEV
                     WGRGALDMKGQVAASAVALASLAREGFEPAGDLIFAATADEEVGDGFGAQWLCETHPD
                     AVRCDYLINEGSGERVVLGGKPFYTCSVAEKMSAPFRLRVHGRSGHASMPGIADNALV
                     KAAPLITALGEYVPEQHLTPEVEALLEAVTGERPASPQVALEQARAIGEFFAEMVEPL
                     LSLTLSPTMISASKKRNVIPAICDVIVDSRLLPETTTAEQHATVRTVLRDGDYELESL
                     EAHGGTRSPIETPLWDAVSGWIAEVEPEARPAPICVAGFTDSHWFREAFGTVAYGFFP
                     LRAMDAELAARLIHSADERIPVEDLELAVSFLRYAAQAMLA"
     gene            complement(2482..3207)
                     /locus_tag="VF225_07985"
     CDS             complement(2482..3207)
                     /locus_tag="VF225_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX7145236.1"
                     /translation="MATETHEDFSEPILPGKGASDYERYLRTDDLLALQKSPEEQVHP
                     DELLFQTVHQSSELWLKLGTSEVARATVHLRAGELGAALRLLGRAALCLQFITRQLDM
                     LEQMSPWEYQTVRRVLGHGSGFDSPGFRDVRRVTPPLGEAFEAAVSQAGLDVVEVYVQ
                     GREHEELYQLAEALVEWDERITMWRIRHYKVVARIIGDQVVGTQGTPVEVLGKMIHHN
                     FFPALWRARNQLTARSKQEEDAP"
     gene            3235..4491
                     /locus_tag="VF225_07990"
     CDS             3235..4491
                     /locus_tag="VF225_07990"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7145237.1"
                     /translation="MSDLFSYAAEQRLPGVAPLALRLRPQSLDEFVGQEHVLGESSAL
                     RLAIAEDRVRSAIFYGPPGTGKTTLARIIARATGAEFEELSAVSATVGQVREVLARAR
                     ERLGANGQRTILFLDEIHRFNKAQQDALLPGVEEGVITLIGATTENPYFEVNSALLSR
                     AQVYELLPLSVEELETVARRGAAELGAEIPDELLRQIAIRAGGDARSVHNIVELALQT
                     AEAAGEPLSEAHVDDAARKRPLLYDKTADAHYDFISAFIKSMRGSDPDASVYYLAAML
                     ESGEDARFIARRMIVLASEDIGNADPRALQVAVAAAQAVEHVGLPEARLNLAQAAIYL
                     ARAPKSNASYEAIKRATKDVQERGNIRPPKALADGSYYGRKLGKGEGYIYPHSDPSGF
                     DVDYLPDELKGTTYYEPSGSGEETKG"
     gene            4492..5223
                     /locus_tag="VF225_07995"
     CDS             4492..5223
                     /locus_tag="VF225_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145238.1"
                     /translation="MAEPAAPAQQTVSTLEPERLAEAARDARNDAAPFALAAAAILVA
                     LALVSMHAHWDLLGHRLWWMWLIVAGPYVLLSGTLLLGLARLTRHDRRREIVIALLAL
                     VWVFNVFGVVLLVVSLVAHSGVQMTGRQLLFSGGALWVTDAIAFGLAFWELDSGGPLA
                     RALSNAPRKPDFQFPQDENPELAAEGWAPRLWDYLYVSQTNSIAFSPTDVMPLTRRAK
                     TLMAAEATLSAITLLLVAARAVNIL"
     gene            complement(5237..5908)
                     /locus_tag="VF225_08000"
     CDS             complement(5237..5908)
                     /locus_tag="VF225_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7145239.1"
                     /translation="MLALLYDIHGNLVALDEVLKDAEAVGAKAYLLGGDFASSSPWPR
                     ETIERLRGLPNTTWVRGNGERGRREPPADRPEVLQALSEHDSGLGTDEGWLYSLQAQF
                     EQDGVLYVHGSPLADFESFAPEPGEDDERLLDGVGGKIIVFGHSHQQFRRPGPNETTL
                     INPGSAGMPLDGDVRAAYALRRDDGEFEFRRVEYDVERVAQAWEQVGGTFGTFAAQRI
                     RRGSD"
     gene            5929..7662
                     /locus_tag="VF225_08005"
     CDS             5929..7662
                     /locus_tag="VF225_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7145240.1"
                     /translation="MKIVCLVKQVPRGDAINFDPETKTLRREGVPLLLNPFDAGAVAL
                     AAGLKERVGGEVIAMTMGPPQAADALRTCLALGADRCIHLSDRVFAVADTLGTSRTLA
                     LAIQKEGGVDLVLCGRKTVDSETWQVPPETAAFLGWPHLTSVVDVELIDGRVRATRET
                     DAGYDTYELDLPAVLSLVSAFEAENSSDGSVEVWTANDLVGDVREDDKRFGQTGSPTR
                     VLAVHDVTPERKGELAKSAAEVIGKVRDLLADLPPGATQWDKPARLGEKPGKNYDAWT
                     FVETVDGQATRASLELIAKSRELAGKLGGESVALVMGKDAAAVGETLGRHGAERVVLV
                     EDERLDDYHSELWTAALVQILRRLRPHALAIPATARGRDLGPRAAGELELGMTGDCVN
                     LGIDRGGRLIQTKPAYGGNIVSVIMGRTTPQLATVRPRMFGVLEPREETARVERFELG
                     PLPEPRSTLVDRRPAPAFDLDENDVVVLLGPDAPAGWAPEGVAVGGTREVCARGDLPG
                     NRQIGLYGRPVAPRVLIAVGVPGDFEQLTGIVKSAVVVSVGGGAGMEQAADVVFRGEP
                     SDVIDALAHER"
     gene            7662..8447
                     /locus_tag="VF225_08010"
     CDS             7662..8447
                     /locus_tag="VF225_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145241.1"
                     /translation="MELNLHEWGDPSAPPVVCLHGVNAHGRRFRRLAEERLARRFRVL
                     APDLRGHGNSGWEPPWTFSTHAHDVLETLDAAGVRQAQWVGHSFGGRLVLELAVLAPE
                     RVERAALLDPAIQLLPHVGFDFAERERADRAFGSTEAAIAERLETGSPTPREHLEEEM
                     REHLVQHSDGEWRFRYCQSAVVSMYGELTTPPPPPETLRVPTLLVHAGEFGLVREDQI
                     DAYRLALGDLIEVIEVPGGHMVYWDAYEETADAVGRFLEDPGP"
     gene            complement(8427..9338)
                     /gene="rocF"
                     /locus_tag="VF225_08015"
     CDS             complement(8427..9338)
                     /gene="rocF"
                     /locus_tag="VF225_08015"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX7145242.1"
                     /translation="MLSQPLRRKVAVIGANLDLGAGRRGVDMGPSAIRYAGLAARLDE
                     LGYEYVDLGNVETAVPEATEAGDEHARFLPQIKETSQRIAQLVSRTARDDFVPVVLGG
                     DHSVALGTLAGLREVHGPGGAIWIDAHGDLNSPETSPSGNVHGMVLAAALGLAGDRFR
                     DDGWGLPALEPGRVALVGVRSLDDGERALLGDQDVKVFTMSDLDRLGVERSVREALAH
                     VAGPGFVHVSLDMDAIDPDVAPGVGTPVRGGLSYREAHLAMELVAESGLASSLDVVEV
                     NPVLDRENETGKLAVELIASALGARIL"
     gene            9365..9757
                     /locus_tag="VF225_08020"
     CDS             9365..9757
                     /locus_tag="VF225_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145243.1"
                     /translation="MGEADAAGTIRVLLADDDAPFLAALSPLIERQPELAVVGTAVDG
                     MAAIELADELDPDAVVIDLHMPRLDGVSAVARLRHDHPSMCVIALTADEHRALHDAVT
                     EAGADAVLMKNEFVDLLIARLAAAKAGV"
     gene            9782..9854
                     /locus_tag="VF225_08025"
     tRNA            9782..9854
                     /locus_tag="VF225_08025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9815..9817,aa:Phe,seq:gaa)
     gene            10033..10620
                     /locus_tag="VF225_08030"
     CDS             10033..10620
                     /locus_tag="VF225_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145244.1"
                     /translation="MRAARFFALACLAGGIAGSVPALGLSDSAPGAKSAQQACGRVEF
                     LSGLEAVFGRRKTHQQALAFRTQITARGFVNANIIEGCDGFRVVVRGIDTFDVGVDLQ
                     SEARREGFAVTLECIQAKPLGRLEAIFGHGRDRPSATAIVNRADAAGFPGVKLRNDPC
                     GGFEAYLAGFEDQSEAEAFVAQAKARGFNVVLERN"
     gene            complement(10692..11321)
                     /locus_tag="VF225_08035"
     CDS             complement(10692..11321)
                     /locus_tag="VF225_08035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7145245.1"
                     /translation="MNRRELGQKFARLATNAVLRSPRLWRFFRPLVRKQFDAIAAGWD
                     TMRDPTHLAPYEAALAAVDPAPSKALDLGTGTGQGAFAIARRFPHTQVVGVDLAEGML
                     AEARRKTPAELADRIRFESGDASALPFEDGSFALVAHANMIPFFDELERVLAPGGQVV
                     FAFSSGPGTPIYVMPERLREELRRRGFSEFAEFAAGNGTALLARKEVRA"
     gene            complement(11318..12130)
                     /locus_tag="VF225_08040"
     CDS             complement(11318..12130)
                     /locus_tag="VF225_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX7145246.1"
                     /translation="MASFITFLTDFGLQDDFVGTCHGVMKRIAPEVEIIDITHGIAPQ
                     AVLQGALTLANTLPFMPEGVHLAVVDPGVGGARRALALRDAQGRMYVGPDNGLLIPAA
                     EKLGGITDAHELANPEYALESVSRTFHGRDLFSPAAAHLALGVPLSELGPPIDPDALA
                     RLDIPRPDVGSTRIHSTVISIDRFGNIGLNLDRSHLDEAAVVPGTRVELQVGPERYYA
                     VAARTFADARPGDIILYEDAYRNISIAINGGNAAAMFGIKEGQDIRIHLDVF"
     gene            complement(12106..12753)
                     /locus_tag="VF225_08045"
     CDS             complement(12106..12753)
                     /locus_tag="VF225_08045"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7145247.1"
                     /translation="MRVIVTRPRTQAEPLLNALRAQGFEPVACPVIEIEAIDNGPIDV
                     SDYDWVIVTSANGAAELDVRHTGELPRVAAIGETTAAALAARGIAVDFVPSLASQEGL
                     LAELPRPVGRALFVGAERARRLLVDELPADYRAVYRTVELTPSPPDGELVLLASPSAA
                     RAWAKLGSTVPAITIGPQTTAAALAAGLAVVAEAGTQDVVGLVDSAARWRASSRS"
     gene            complement(12755..13582)
                     /gene="hemC"
                     /locus_tag="VF225_08050"
     CDS             complement(12755..13582)
                     /gene="hemC"
                     /locus_tag="VF225_08050"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7145248.1"
                     /translation="MLIRVGSRGSRLALTQAELASARLRGPGVEIALVPLTTAGDRDR
                     TRPFGEIGARGVFVKELEEALLDGRIDVAVHSAKDMTSTDTEGLAVGAYLERDDPRDA
                     LCGTPELRAGMRIGTASVRRRAQLLALEPTLSIEPLRGNIETRLRKRGERGLDAIVLA
                     ACGLERLNLAGEIGRRFDPEELVPEAGQGALALQVRAGEEHLVGAADHGETRRRVETE
                     RACVAVIGGGCLAPIAAYHDGARLTALVAAEDGSWIERRSGDDPGEVAAALMPFVSA"
     gene            complement(13694..14920)
                     /gene="hemA"
                     /locus_tag="VF225_08055"
     CDS             complement(13694..14920)
                     /gene="hemA"
                     /locus_tag="VF225_08055"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7145249.1"
                     /translation="MSIVLVGISHHQAPVELRERAALDRQRAAELAEALAGGRSEAVV
                     LSTCNRTELYLADESADAAEEKAEVALLALESKLGPALYRLRDEEAALHLFRVAAGLD
                     SMIPGEGEILGQVRAAHESGATGPLLDRLFRQAVQAGRKARTQTAIGESPASVSSAAA
                     ALAEQVFGDLEGRSVLVVGAGKVGELAIRNLVSRGATIASVANRTGQRAEDLTERFGG
                     EALPLERIDEELVRADVVLSSTSAPGWILTREQVEQALRARKGRPLFLIDLAVPRDLD
                     PAIHELEGCYLYDIDDLEAVVAETLAGRRREAARAETIVAEEADRFRAWQASLDVVPA
                     ITSLRARAEQIRDAELSRAKLNDAERRAAESVTAAVLNKLLHLPTIRMKQAAAAADGV
                     IYADAVRHLFGLEDER"
     gene            complement(14917..>15341)
                     /gene="ccsA"
                     /locus_tag="VF225_08060"
     CDS             complement(14917..>15341)
                     /gene="ccsA"
                     /locus_tag="VF225_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7145250.1"
                     /translation="FLVLHVGLVLAAFAGFTLAAALSALYLWQERRLKRRESSILRLR
                     APALSRLDEVVARTIAIALPALTLGIAVGIVRLRERGGGFDALMAVTVVTWAIYSAYL
                     VLRHFSGWRGRRAAYLVLAGFVFVVVVRLGLPVTHFPT"
BASE COUNT         2480 a         5289 c         5206 g         2366 t
ORIGIN      
        1 cgtgtagtcg ccctcgatgg tcatcaccgt ctccgaaggc gccacgcggt cgccgtcgta
       61 gagcgcgtgc acggtgagct cgtcccagtc gtacgagcag agcttgagga tcgcgatcgc
      121 ctcgtccatg ccgccgaggt atgcgtcgtt tttctggaag acctgcatca cgacgcgcgg
      181 acgacgcgcg tcctgcaaga gtgtcgctcg cgcgtgattg aagtacgcgt ctgcgtagta
      241 gccctcgcgc atcttctcga ccggcaggtc gaagatctcg ggcggaagcc gctcgcgagt
      301 tcgttcctcg agcgaagcca tggttattcc tgctcgagct cgaggcacgc ggcgagtgcg
      361 gcctcggcga cctcgatctg ctccggcgtc ggctccgcgg tcgcgaggcg atgctggagc
      421 tcgtggcccg gcttggcgag agcgcgtgcg aggcggttct cgggattgcg caccatccag
      481 ccgaagatct cggtcgatgc agccagcgcc acgagctggg cgccgagccg cgccggcctg
      541 cggagatggg cgggtgcgcg cgccgccagc acgttgccga ttgccgtggt cgcgagcaat
      601 ggaccgatca ggtgcgaccc gcagcggttg tgctccttcg ccgcgcgctt gccctgctcg
      661 tacgagccga tcgagatgtg ctctgccccg tgataggcag caacgtcgga cccgcgcagc
      721 gcaagtgcgg ctggggcgac cgagagcaga ccgctgacga cctcctgaac cgccggaccg
      781 accgagcgcg aaccgcgcag gagcctcagg accgtggcgc taccgaccat cgcagccacg
      841 accaccggac gctgaaacgg cagctttgcc tcggggagcg cgcgcttgat ctccgggatg
      901 atcgccatcg catcgagtag gcgcacaggg ccacgaagca acgggctctc gaccttgctc
      961 gcccggagcc gcttgcgctg cgcggcgacc ttcaggcgcc catcttccgt gcggatcgca
     1021 caggcccagg acctggggcc gtggacgagc acgccgttgg ccagcgccat cccgccgagc
     1081 cggatcttct cgtcgctcat ctcatcgagg ttatcgactc agagtcgaat tccgcaaacg
     1141 ccaagcgagt cctaggccag catggcctgg gctgcgtacc tcaggaagct cacagcgagc
     1201 tccaagtcct ccaccggaat gcgctcgtcg gctgagtgga tcaggcgcgc cgccagctcg
     1261 gcatccatcg cacgcaacgg gaagaagccg taggcgacgg tgccaaaggc ctcgcggaac
     1321 cagtggctgt cggtgaaccc cgcgacgcag atcggcgccg gcctcgcctc gggctcgacc
     1381 tcggcgatcc agccggagac ggcgtcccac agcggcgtct cgatcggcga gcgcgttccc
     1441 ccgtgtgcct cgagcgactc cagctcgtag tcgccgtcgc ggagcacggt gcgcacggtc
     1501 gcgtgctgct cggcagtcgt cgtctccggc agcagtcgcg agtcgacgat aacgtcgcag
     1561 atcgcaggaa tgacattccg cttcttcgag gcgctgatca tcgtcgggga aagcgtcagg
     1621 gacagcagcg gctcgaccat ctccgcaaag aactcgccga tcgcgcgagc ctgctcgagc
     1681 gccacctgcg gcgacgcggg ccgctcgccc gtgactgctt ccagcagtgc ctccacctcc
     1741 ggtgtgaggt gttgctccgg cacgtactcg ccgagcgccg tgatcaacgg cgcggccttc
     1801 accagcgcgt tgtctgcgat ccccggcatc gacgcatgcc cgctgcgtcc atgcacgcgt
     1861 agccggaagg gcgcgctcat cttctcggca acggagcagg tgtagaacgg cttaccgccg
     1921 agcacgaccc gctcgccgga cccttcgttg atcaggtaat cgcaacgaac cgcatccgga
     1981 tgcgtctcgc acaaccactg cgccccgaat ccatcgccga cctcctcgtc ggcagtcgcc
     2041 gcgaagatca ggtcgcctgc cggctcgaag ccctcccgcg cgagcgaggc aagcgcaacc
     2101 gcacttgcgg cgacctgccc cttcatgtcg agcgcgccgc gaccccacac ctcgccgtcg
     2161 cgcagctcac caccgaacgg gtccgcgctc cactccgacg cgtccgcgag cacgacgtcg
     2221 gtgtgcgaga ggaagagcag ggtcggcccg tcgccccgcc ccggaatgcg cgcaacgagg
     2281 ttggcacgtt cgggaatgcg cgcatacagc tccacctgcg caccgcttgc ctccaggtag
     2341 ctccgaagaa gctcggccgc agcggtctcg ttgcccggcg gattcgtcgt gtcgacgcgg
     2401 atcaactcct gcaggagctc ggtgacctcg tcgcgggccg agacggtcgc cattacagcg
     2461 tcttcagcgg cccaaaggac gtcacggagc gtcttcttct tgcttcgagc gggcggtcag
     2521 ctggttccgc gcacgccaca gcgcggggaa gaagttgtga tggatcatct ttccgagcac
     2581 ttcgaccggt gtcccctgcg tcccgacgac ctggtcgccg atgatgcggg cgacgacctt
     2641 gtaatggcga atccgccaca tcgtgatccg ctcgtcccat tcgaccaacg cctccgcgag
     2701 ctggtagagc tcctcgtgct cgcgcccctg cacgtacacc tcgaccacgt cgaggccggc
     2761 ctgcgacacc gccgcctcga atgcttcgcc cagcggcgga gtgacccgcc gaacgtcgcg
     2821 aaagccgggt gagtcgaagc cgctgccgtg tccgagcaca cgacgaacgg tctggtattc
     2881 ccacggcgac atctgctcga gcatgtcgag ctggcgcgtg atgaattgca ggcagagtgc
     2941 cgcgcgcccg agcagccgca gcgccgcccc aagctcgccc gcgcgcagat ggacggttgc
     3001 ccgggcgacc tccgaggtgc cgagcttgag ccagagctcc gaagactggt gcaccgtctg
     3061 gaacagcagt tcgtccgggt ggacctgctc ctccggtgac ttctgcaaag caagcaggtc
     3121 gtcggtccgc aggtagcgct cgtagtccga cgcccccttc ccgggcagga tcggctcgga
     3181 gaaatcctcg tgagtctcgg tcgccaaggg cctacggagc atacttctcc tgcaatgagc
     3241 gacctcttct cctatgcggc ggagcagcgg cttccgggtg tcgcaccact cgcgctgaga
     3301 ctgcgaccgc agtcgctcga cgaattcgtc gggcaggagc acgtgctcgg cgagagctcg
     3361 gcgctgcggc tggcgatcgc ggaggaccgg gtacggtctg ccatcttcta cggaccgccc
     3421 ggcaccggga agacgaccct tgcgcgcatc atcgcgcgcg cgaccggcgc agagttcgag
     3481 gagctctcgg cggtatcggc gaccgtcggc caggtacggg aggtactcgc gcgcgcgcgc
     3541 gagcggctcg gagcgaacgg acagcgcacg atcttgttcc tcgacgagat ccaccgcttc
     3601 aacaaggccc agcaggacgc gctccttccc ggtgtcgagg agggagtgat cacgttgatc
     3661 ggcgcgacga cggagaaccc ctacttcgag gtcaactccg cgctgctgtc tcgagcccag
     3721 gtgtacgagc tcttgccgct gtccgtggag gagctcgaga cggtcgcgcg ccgcggggcg
     3781 gcggagctgg gcgccgagat tcccgacgaa ctgctcagac agatcgccat ccgtgcgggc
     3841 ggcgacgccc gctccgtcca caacatcgtc gagctcgcac tgcaaactgc ggaggccgca
     3901 ggggagccgc tctccgaggc tcacgtcgac gacgcggcgc gcaagcggcc gctgctctac
     3961 gacaagaccg ccgatgcgca ctacgacttc atctccgctt tcatcaagtc gatgcggggg
     4021 tcggaccccg acgcgagcgt ctactacctc gccgcgatgc tcgagagcgg cgaagacgca
     4081 cggttcatcg cgcggcgcat gatcgtgctc gcatccgagg acatcggcaa cgcggatccg
     4141 cgggcgctgc aggtcgcagt ggccgcagcc caggcggtcg agcacgtggg gctgccggaa
     4201 gcgcgcttga acctcgcgca ggcggcgatc tatctcgcac gcgctccgaa gtcgaacgcg
     4261 agctacgaag caatcaagcg cgcgacaaag gacgtccagg agcggggaaa catccggccg
     4321 ccgaaggcgc tggccgacgg cagttactac ggacgcaagc tcggcaaagg agagggttac
     4381 atctatccgc actccgatcc gtcgggattc gacgtcgact acctcccgga cgagctgaag
     4441 gggacgacgt actacgagcc gtcggggagc ggcgaggaga cgaagggcta gatggccgaa
     4501 cccgcagcac ccgcgcagca gacggtgtca actctggagc cggagcgtct ggccgaggcg
     4561 gcgcgcgacg cacggaacga cgctgcaccc ttcgcgctcg cggcggccgc aatcctcgtg
     4621 gctctggcgc tcgtgagcat gcacgcccac tgggacctgc tcgggcaccg cctgtggtgg
     4681 atgtggctga tcgtcgccgg tccttacgtc ttgctttccg ggacgctgct cctgggcttg
     4741 gcccgcctca cccggcatga ccgcaggcgg gagatcgtga tcgcgctgct tgctctcgtc
     4801 tgggtcttca acgtcttcgg tgtcgtcctg ctcgtcgtct ccctggtcgc tcactcgggc
     4861 gtgcagatga cgggccggca gctgctgttc agcggcggcg cactgtgggt caccgacgcc
     4921 atcgcgttcg ggctcgcctt ctgggagctg gactccggtg gtccgctggc ccgggcgctc
     4981 tcgaacgcgc cccgcaagcc cgacttccag ttcccccagg acgagaaccc cgaactggcg
     5041 gcagagggct gggcgccgcg cctgtgggat tacctctacg tgtctcagac caactcgatc
     5101 gccttcagcc ccaccgacgt gatgccgctc acgcgacgcg ccaagacgtt gatggcggcc
     5161 gaagcgacgc tttctgcgat caccctgctc ctggtcgccg cgcgcgccgt gaacatcctc
     5221 taggtgaagt cgacgcctaa tcggagccgc ggcggatccg ctgcgccgcg aacgtgccga
     5281 acgtgccgcc gacctgctcc cacgcttgcg caacgcgctc gacgtcgtac tcgacgcggc
     5341 ggaactcgaa ctcgccgtcg tctctgcgca gcgcatacgc cgcgcgcacg tcgccgtcaa
     5401 gcggcatgcc ggcgctgcct gggttgatca gcgtcgtttc gttcggcccg ggcctgcgga
     5461 actgctggtg gctgtggccg aagacgatga tcttgccgcc gacgccgtcc agcaatcgct
     5521 cgtcgtcctc accgggctca ggcgcgaagc tctcgaagtc cgcaaggggc gagccgtgga
     5581 cgtagaggac accgtcctgc tcgaactgcg cctgcaagga gtagagccag ccctcgtccg
     5641 tgcccagccc ggagtcgtgc tcggagagcg cctgcagcac ctcgggccga tctgccgggg
     5701 gctcgcgccg cccgcgctcg ccgttaccgc gaacccaggt cgtgttcgga aggccgcgca
     5761 gccgctcgat cgtctcgcgc ggccacggcg acgacgacgc gaagtccccg ccgaggagat
     5821 aggccttggc tcccacggcc tctgcgtcct tcaggacctc gtcgagggca accaggttgc
     5881 cgtggatgtc gtagaggaga gcgagcacgg ctgattaggc tcccacaggt gaagatcgtc
     5941 tgcctcgtca aacaggtccc tcgcggcgac gcgatcaact tcgatccgga gacgaagact
     6001 ctgcgccgtg agggcgtgcc actgctcctc aatccgttcg acgccggggc cgttgccctg
     6061 gctgcagggc tgaaggagcg tgtgggcggc gaggtgatcg cgatgaccat gggcccacca
     6121 caggccgcgg acgcattacg cacgtgcctc gctctcggcg ccgatcggtg cattcatctg
     6181 tccgaccggg tgttcgccgt cgccgacaca ctcggcacat cgcgcacgct cgccctggcg
     6241 atccagaagg agggtggcgt cgatctcgtc ctctgcgggc gcaagaccgt ggactcggag
     6301 acgtggcaag tgccgccgga gaccgcggcg ttcctcggct ggccgcacct gacgagcgtc
     6361 gtcgacgtcg aactcattga cggccgcgtc cgtgcgaccc gtgaaacgga tgcggggtac
     6421 gacacgtacg agctggatct tccagcggtg ctctcgctcg tctcggcgtt cgaggcggag
     6481 aactcgagcg acggaagcgt cgaggtctgg accgcaaacg acctcgtcgg cgacgtccgt
     6541 gaggacgaca agcggttcgg ccagaccggg tcgccgacgc gcgtgctcgc cgtgcacgac
     6601 gtcacgcccg aacgcaaggg tgagctcgcg aagagcgccg ccgaggtgat cggcaaggtc
     6661 cgcgaccttc tggccgacct tcctcccggg gcgacgcagt gggacaagcc ggcacgcctg
     6721 ggcgagaagc ccgggaagaa ctacgacgcg tggaccttcg tcgagaccgt ggacggtcaa
     6781 gcaaccagag cctcgctcga attgatcgcc aaaagccgcg agctcgctgg gaagctcggc
     6841 ggagagagtg tcgcgctcgt catgggcaaa gacgcagcag cggtcggcga gaccctcggc
     6901 aggcacggcg ccgagcgcgt cgtgctcgtc gaggacgagc gcctcgacga ttaccactcg
     6961 gagctctgga ccgcagcgct cgttcagatt ctccgacggc ttcggccgca cgcgcttgcg
     7021 atccccgcaa cggcacgcgg ccgggatcta gggccccggg cagcaggaga gctggagctg
     7081 ggcatgactg gcgactgcgt gaatctcgga atcgatcggg gtggacgcct catccagacg
     7141 aagcccgcgt acggcgggaa catcgtctcg gtgatcatgg gccgaaccac accgcagctc
     7201 gcgaccgtgc gcccgaggat gttcggcgtg ctcgaaccgc gcgaagagac ggcaagggtc
     7261 gagcgcttcg agctcggccc gctgccggag ccccgctcca cgctcgtgga ccggagaccc
     7321 gcacctgcgt tcgatctcga cgagaacgac gtcgtcgtgc tgctcggtcc cgacgcaccg
     7381 gcgggctggg ctcccgaagg agtcgccgtc ggagggacgc gcgaggtctg cgcgcggggt
     7441 gaccttcccg gcaaccgcca gatcggcctc tacggccgcc ccgtggcacc gcgcgtcctc
     7501 atcgccgtag gcgtcccggg agacttcgag cagctgaccg ggatcgtcaa gtcggcggtc
     7561 gtcgtgtctg ttggcggcgg ggccgggatg gagcaggcgg cagacgtcgt cttccggggc
     7621 gagcctagcg acgtcatcga cgcgcttgcg cacgagcgct gatggagctc aatctgcacg
     7681 agtggggcga tccatctgct cccccggtcg tctgtctcca cggcgtgaac gcgcacgggc
     7741 gccgctttcg caggctcgcc gaggagcggc ttgcgcgacg gttccgcgtt ctggctccgg
     7801 acttgcgtgg acatggcaac tccggctggg aaccgccgtg gacgttctcc acgcacgcac
     7861 acgacgtcct cgagacgctc gacgcagccg gagtccggca ggcgcagtgg gtcggccatt
     7921 cgttcggcgg ccggctcgtc ctcgaactgg ccgtcctcgc accggagcgc gtcgagcgcg
     7981 ccgcactgct cgaccccgca atccagcttc ttccgcacgt cggtttcgac ttcgccgaac
     8041 gcgagcgggc cgatcgtgct ttcggctcca ccgaggctgc gatcgcggaa cggctcgaga
     8101 ccggctcgcc gacaccgcgc gagcatctgg aagaggagat gcgcgagcac ctcgtgcaac
     8161 actcggatgg cgagtggcgc ttccgctact gccagagcgc ggtggtatcg atgtacggcg
     8221 agctcaccac gccgcccccg ccgccggaga ctttgcgcgt gccgaccttg ctcgtccacg
     8281 ccggcgagtt cggcctcgtg cgcgaggatc agatcgacgc ttatcggctg gccctcggcg
     8341 acctgatcga agtgatcgag gtgccgggag gccacatggt ctactgggac gcgtacgagg
     8401 agaccgcgga cgcggttggg cggttcttag aggatccggg cccctaacgc actggcgatg
     8461 agctcgactg caagctttcc cgtttcgttc tcgcggtcca gcaccgggtt cacctcgacc
     8521 acgtcgagcg acgacgcgag ccccgactcc gcgacgagtt ccatggcgag gtgcgcctcc
     8581 cggtaggaga ggccgccgcg caccggcgtc cccactccgg gcgcaacgtc gggatcgatc
     8641 gcatccatgt cgaggctcac gtgaacgaag ccgggtccgg cgacgtgggc gagcgcctcg
     8701 cggaccgagc gctccacccc gaggcggtcg agatcgctca tcgtgaacac cttcacgtcc
     8761 tggtctccga gcagcgcgcg ctcaccgtcg tccaatgagc gcactccaac gagcgcaaca
     8821 cgtcccggct cgagcgcggg caaaccccaa ccgtcgtcgc ggaagcgatc gccggcgaga
     8881 ccgagcgcgg cggcgagcac cattccgtgg acgtttccgc tcgggctggt ctcgggactg
     8941 ttcagatcgc cgtgcgcgtc gatccagatg gcgccgccgg ggccgtggac ctcacgcagg
     9001 cccgcgagcg ttcccaaggc aaccgaatgg tcaccgccca gcacgaccgg aacgaagtca
     9061 tccctcgccg tgcgcgaaac gagctgggcg atccgctgcg acgtttcctt gatctgaggc
     9121 agaaagcggg catgttcgtc cccggcctcg gttgcctccg gcaccgcggt ctcgacgttg
     9181 cccagatcca cgtactcgta ccccagctcg tcgaggcggg cagccaaacc ggcataacgg
     9241 atcgcggaag gacccatgtc aacgccgcgg cgaccggccc ccaggtcgag gttcgcgccg
     9301 atgacggcga ctttgcgtcg aagcggttgc gaaagcacga ccaacaaggc taacttgcgg
     9361 acccatgggg gaagccgatg cggcggggac gatccgagtt ctccttgccg acgacgacgc
     9421 cccgttcctg gcggcgctct cgccactgat cgagcggcag cccgagcttg cggtcgtggg
     9481 caccgctgtc gacggcatgg ctgcgatcga gctcgcggac gagctcgatc ctgacgctgt
     9541 ggtcatcgac ctgcacatgc cgagactcga cggcgtgtct gccgtcgcgc ggctgcgcca
     9601 cgaccatccg agtatgtgcg tgatcgcgtt gaccgcagac gagcatcgcg cactccacga
     9661 cgccgtcaca gaggccggag ccgacgcggt cctgatgaag aacgagttcg tggacttgct
     9721 catagccagg ctcgctgcgg ccaaagcggg cgtgtagcgg cggctagact gcgggccgcc
     9781 gggcgaggta gctcagttgg tagagcacac ggctgaaaac cgtggtgtcg ccggttcgat
     9841 tccggccctc gccatcaccc gacgaagtcc ggccggcgcc ttcgcgccgc ccatgacgac
     9901 ttcgtcgggt ttctcgtttc gatccggcgc gtaagtggtc gcgccgggca aagcccggcg
     9961 ctccgcggct ggttctaaag gattcgttaa cactgcggct ggcctccgcg gggtctggta
    10021 cgactgtgcg ccgtgagggc ggcacgtttc tttgcgctgg cttgcctggc cggcgggatt
    10081 gcgggctcgg tgccggctct ggggctttcg gactcggcgc ccggcgccaa gtctgcgcag
    10141 caggcatgcg gacgtgtcga gttcctcagc ggcctggagg cggtcttcgg tcggcgtaag
    10201 acgcaccagc aagcgctggc gttccgtact cagatcacgg cccgcgggtt cgtgaacgca
    10261 aacatcatcg agggctgcga cggcttccgg gtcgtcgttc gcggcatcga caccttcgac
    10321 gtgggagtcg acctccagag cgaggctcgc cgcgaaggct tcgccgtcac gctcgagtgc
    10381 atccaggcga aaccactggg gcggctcgag gcgatcttcg ggcacggacg tgaccgacct
    10441 agcgcgacgg cgatcgtgaa tcgcgccgat gctgccggct ttcccggcgt caagctgcgt
    10501 aacgaccctt gcggcggatt cgaggcttac ctcgcgggat tcgaggatca gagcgaggcc
    10561 gaagcattcg tcgcccaggc gaaggcccgc ggcttcaatg tcgtgctcga gcgcaactag
    10621 tagcgctgca cataaagaaa ggccgtccgg accggtgata ccgcgccgcg aacgaccttg
    10681 tggaccgaag cttaggcgcg gacctctttg cgggcaagca gggcagtgcc gttgccggcc
    10741 gcgaactccg caaactccga gaatccacgc cgtcggagct cttccctcag ccgctcgggc
    10801 atgacgtaga tcggtgtccc cgggccggac gaaaacgcga acaccacctg gccgccggga
    10861 gcgagcacgc gctcgagctc gtcgaagaac gggatcatgt ttgcgtgcgc gacgagcgcg
    10921 aacgaaccgt cttcgaacgg cagtgcggac gcgtcgccgc tctcgaagcg gatacggtcg
    10981 gcgagctcgg caggtgtctt gcgccgcgct tccgccagca tcccttcggc gagatcaacg
    11041 ccgaccacct gcgtatgtgg aaagcgccgg gcaattgcga aggccccttg gccggtgccc
    11101 gtgcctaggt cgagcgcttt cgaaggagcc gggtcgaccg cggccagcgc ggcctcgtac
    11161 ggcgcgaggt gcgtggggtc gcgcatcgtg tcccagcccg cggcgatggc gtcgaactgc
    11221 ttgcgcacga gcggacgaaa gaaccgccag agccgcgggc tgcgcagcac cgcgttcgtc
    11281 gccagccgcg caaacttctg gccgagctct cgacgattca aaacacgtcc aggtgaatgc
    11341 gaatgtcctg gccctccttg attccgaaca tggccgcggc gttgcctccg ttgatcgcga
    11401 tcgagatgtt ccggtaggcg tcctcgtaga ggatgatgtc gccaggccgt gcgtcggcga
    11461 acgtgcgcgc cgcgaccgcg tagtaacgct cggggcctac ctgcagctcg acacgcgttc
    11521 ccggcacgac ggcggcctcg tcgaggtgcg atcggtcgag attgaggccg atgttcccga
    11581 agcggtcgat cgaaatgacc gtcgagtgga tgcgcgtcga gccgacgtcc ggccgtggga
    11641 tgtcgagacg agccaaggca tccgggtcga tcggcgggcc gagctcactg agcggaacgc
    11701 cgagtgcaag atgcgcggcc gcaggagaga acaggtcacg gccgtggaac gtgcgcgaga
    11761 ccgactcgag cgcgtactcg ggattcgcga gctcgtgggc gtcggtgatc ccgcccagct
    11821 tctcggccgc gggaatgagc agcccgttgt ccggtccgac gtacatgcgc ccttgtgcat
    11881 cacggagagc gagcgcgcgc cgcgcgccgc cgaccccggg atcgacgacc gcaaggtgga
    11941 ccccctcggg catgaacggc agcgtattcg ccaaggtgag cgctccctgg agaacggcct
    12001 gaggcgcgat gccgtgcgtg atgtcgatga tctccacctc cggcgcgatc cgcttcatca
    12061 ccccgtgaca ggtaccgacg aagtcgtcct gcaggccgaa gtccgtcagg aacgtgatga
    12121 agctcgccac cgcgcggcgg agtctacgag gcctacgaca tcctgcgtgc cggcttccgc
    12181 caccacggcc aggcctgcag cgagcgccgc ggccgtcgtc tgcggtccga tcgtgatcgc
    12241 tgggacggtc gagccgagct tcgcccacgc ccgcgcagcg gagggcgagg caagcagcac
    12301 cagctcgccg tcgggcggcg acggtgtcag ctccacggtg cggtagacag cgcggtagtc
    12361 ggcaggaagc tcgtcgacga gcagccggcg cgcacgctcg gcgcctacga agagggcacg
    12421 acccaccggc cgcggcagct cggccaggag cccttcctgc gacgccaggg agggcacgaa
    12481 gtcgactgcg atgccccgcg ccgcaagtgc ggccgcagtc gtctctccga tggctgcgac
    12541 acgcggcaac tcgccggtgt gtcgcacgtc gagctcagcg gcgccgttcg cgctcgtgac
    12601 gatcacccag tcgtagtcgc tgacgtcgat cgggccgttg tcgatcgcct cgatttcgat
    12661 taccggacat gcgaccggct cgaacccctg agcgcgcagg gcgttgagca aaggttctgc
    12721 ctgcgtcctc ggccgcgtga cgatcacgcg catgtcaggc cgagacgaac ggcatcagcg
    12781 cggctgcgac ctcgccggga tcgtcaccgc ttcgacgctc gatccagctg ccgtcctcgg
    12841 ccgcgacgag cgcggtcagg cgtgcgccgt cgtgatacgc ggcgatcgga gcgaggcacc
    12901 cgcccccgat gacggcgacg caggcgcgct cggtctcgac gcgccgccgc gtctcgccat
    12961 ggtcggcggc tcccacaagg tgctcttcgc ccgcccgcac ttgaagcgcc agcgcaccct
    13021 ggcctgcctc cgggaccagc tcttccggat cgaagcggcg gccgatctcg ccggcgaggt
    13081 tgagacgttc gaggccgcac gccgccagaa cgatcgcgtc gagcccgcgc tcgccgcgtt
    13141 tccgcagtcg cgtctcgatg ttgccgcgca agggctcgat cgagagggtc ggctcgaggg
    13201 cgagcagctg cgccctccgc cggaccgacg cagtgccgat ccgcattccc gcacgcagct
    13261 ccggcgttcc gcaaagcgcg tcgcgaggat cgtcgcgctc caggtacgcg ccgaccgcaa
    13321 ggccttccgt gtcggtggag gtcatgtcct tcgcggaatg cacggcgacg tcgatgcgac
    13381 cgtcgagcag cgcttcttcc agctctttga cgaagacgcc gcgcgctccg atctccccga
    13441 acgggcgtgt gcggtcgcgg tcgccggcgg tcgtgagggg aacgagcgcg atctcgacac
    13501 ccggcccgcg cagccgggcc gacgccagct cggcctgcgt gagcgcgagc ctactgccgc
    13561 gggaaccgac gcggatcagc acggcaccag gtgcgcttga cccgcgtctg gccgcatggc
    13621 ctgcgccgcc gcacgcgcac gaatgcggtc ggagcctgca cggggagcgg ccgcccgatc
    13681 aggttcgcgg acttcagcgc tcgtcctcga gaccgaagag gtgacgcacc gcatcagcat
    13741 aaatcacgcc gtcggctgcc gcggccgcct gtttcatccg aattgtcggc agatgcagga
    13801 gcttgttcag gaccgccgcg gttacggact ctgcagcgcg ccgctcggcg tcgttcagct
    13861 tcgctcggct cagctcggcg tcgcggatct gttcggcccg tgcgcggaga gaggtgatcg
    13921 ccggaacgac gtcgagggat gcctgccacg cgcgaaaccg atccgcttcc tccgccacga
    13981 tggtctccgc acgcgcggcc tcgcggcgac gcccggccag cgtctcggcc acgaccgctt
    14041 cgagatcgtc gatgtcgtag aggtagcagc cctcgagctc gtggatggct ggatcgagat
    14101 cgcgcggcac ggcgagatcg atcaggaaga gcgggcggcc cttgcgggcg cgcaatgcct
    14161 gctcgacctg ctcgcgcgtg agaatccaac cgggagcgct cgtcgacgag agcacgacgt
    14221 ccgcgcgtac gagctcctcg tcgatgcgct cgagcggcag cgcctccccg ccgaatcgct
    14281 ccgtcaggtc ttccgcgcgc tgccctgtgc ggttcgcgac cgacgcgatg gtcgcgccac
    14341 gcgagacgag attccggatc gccagctcgc cgaccttgcc ggcgccgacg acgaggacac
    14401 tgcgtccctc gagatcgccg aagacctgct cggcgagcgc ggcagctgcc gacgacacag
    14461 acgccgggct ctcgccgatt gccgtctggg ttcgcgcctt acggcctgcc tgcacggcct
    14521 gccggaacag gcggtcgagc agcggtccag tggcgccgga ctcgtgcgcg gcgcgtacct
    14581 gtccgaggat ctccccttcg ccggggatca tcgagtcgag accggcggcg acccgaaaga
    14641 gatggagcgc cgcctcttcg tcgcgcagcc tgtagagagc cgggccgagc ttagactcga
    14701 gggcgagcag cgcgacctcc gccttctctt ccgcagcatc ggcggattca tccgccaggt
    14761 agagctctgt gcggttgcac gtcgagagga ccaccgcttc gctgcggccg ccggcgagcg
    14821 cctccgcgag ctccgccgcg cgttgacggt cgagcgccgc acgctcacgc agctcgacag
    14881 gcgcctggtg atgcgagatg ccgacgagga cgatgctcat gtcgggaagt gggtcacggg
    14941 gagaccgaga cggacgacca cgacgaagac gaagcccgcg agaacgaggt acgccgctcg
    15001 acggccacgc cagccggaga agtgtcgcag tacgaggtag gcgctgtaga tggcccatgt
    15061 gacgacggtg accgccatca gcgcgtcgaa gccgccgcca cgctcgcgca gccgtacgat
    15121 gccgacggcg atcccgagcg tgagcgcggg cagagcgatc gcaatcgtgc gcgccaccac
    15181 ctcgtccagt cgtgaaagcg caggtgcgcg cagacgaagg atcgagctct cgcgccgctt
    15241 cagtcgccgc tcctgccaca ggtagagcgc tgaaagagca gccgcgagcg tgaaccccgc
    15301 gaacgccgcg agcacgagcc cgacgtgcag cacgaggaac a
//
LOCUS       DASVEU010000195         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1689089,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000195 DASVEU010000000
VERSION     DASVEU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689089"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF225_08065"
     CDS             complement(<1..259)
                     /locus_tag="VF225_08065"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7145251.1"
                     /translation="MKADQHDSTWTDDAVAELRRDFGDFFTGRTCLVTGADGFMGSHL
                     TDALVQLDANVHAFVRATSSGALNNIGPLRERLKVHFADLTD"
     gene            350..1768
                     /locus_tag="VF225_08070"
     CDS             350..1768
                     /locus_tag="VF225_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145252.1"
                     /translation="MILSLAAAVAGAFLAHKFGRNKETDGFMAAYGVYLVLVLGAQAF
                     RMVVVPDLTRAEAEGRLGSEFRAYALAFLAVAIPATVVAALFAHFWGELITGRLPKES
                     AAVAADALPWLVPAAFAQLIAALAASALAAKDSYVPAALGFALGGVGGVVFFVLAADS
                     HGLVALAWGLTLNGAIAIALPMIVLFLRGNRLRAHRGVPLRLGHRLWRLLYGAAVPVA
                     LQGLYVIGLRFAAGTGEGSVTSLSYAYLLAATFVSATAFSLSLISAAPLTRRGVNPET
                     AADHVVHSTWISLAFVGAAAGLVALVGGRVITAVLGQAFSGHVGAQLGRLVIFLSPWM
                     VANAAFLIMYPLLFVMHRTRLLIPLAIAAVVVDIPISIAGRALWGLTGVTVALGVSTL
                     LVVLALMASLAPRMLALTVVGLGRLSILVAAATALAFGGASLVLSAVPAAAAGLALYA
                     LLLVAMRQLGLAQAWHYVRALH"
     gene            1795..2925
                     /locus_tag="VF225_08075"
     CDS             1795..2925
                     /locus_tag="VF225_08075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7145253.1"
                     /translation="MSGPEAKNPAPAGISSPEEIDVAALFDRLREELRRGAVHEDSRG
                     AEYASTRALAERFWPVTAEHPAGGGPKGVVKRFLRKLMRWYVEPLAADQRVFNDSVLK
                     LVDALSERADSSTAAREQAERLVRELEERLARLERRGPATGGAPAPATVATQPAAASV
                     PDYFGFESRMRGSVESIRERQRRYVDDLRHDAPVLDIGCGRGELLGLLREAGVEARGI
                     DADADMVAYARGDGLEVEQADLIDYLDRVTAGSVGAIFMGQVVEHLPAPTLVQALRLA
                     AAKLRPGGVLIAETINPLSPIALRNYFADLTHAQPLVPETLELLARQSGFAKTEIRFL
                     NEPAERLTEPDDPVIAANVRRLNELLFGPLDYALVARTATHE"
     gene            3042..4067
                     /locus_tag="VF225_08080"
     CDS             3042..4067
                     /locus_tag="VF225_08080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145254.1"
                     /translation="MRIAVCAPQVPFERGGAEIFADDLVGELRARGHETDLVTVPYKW
                     YPGERVLSQAFLWRLLDLSESDGRPIELAIATKFPSYAVRHPNKVVWLLHQFRQAYEL
                     DRTELGQFGESAEERATRRAVQRLDRVVLGEARKVFATSRNVADRLQRSTGIAAEVMP
                     HPPQELPYRTESYDNFVLSVGRLDRAKRHELLLDALATDSTLHCVIVGDGPDRERLED
                     VARRYGLDGRARFTGRIDEEELAGLYARCLAVYYAPVDEDFGMVPYEAFLAEKPVVTT
                     TDAGGPLEVVTDRATGLVIEPRAAALAEACSWLRDHVDDARAWGQAGKEIARRVSWDE
                     TIARLLA"
     gene            4081..>4898
                     /locus_tag="VF225_08085"
     CDS             4081..>4898
                     /locus_tag="VF225_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7145255.1"
                     /translation="MRVAYYSPLPPERSGIADYSALLLPALERLVEIAIVRRGRTRPV
                     AADVALYHVGNDPEAHGWIVDALKRRPGVVVLHDFVLHHLVAGLTIGRKDGHGYLAAM
                     ERDAGIPGRLLAHGVLDGRVSPPWETRPDEFPLAGEVLANATGLIVHSHYVEDRVLEA
                     GYHGPIWRIEHPAWPAAEVLPVQIEGRPLFGCFGHLNASKRVPQLIEAFGLVRKRHPS
                     AKLLLVGSASPGFDAERFATAGIERLGYVEEQQLWSLMAACDACISLRSPTMGET"
BASE COUNT          685 a         1656 c         1685 g          872 t
ORIGIN      
        1 tgtcggtgag atcggcgaaa tgcaccttga gccgctcgcg cagcgggccg atgttgttca
       61 gcgcgccgct cgacgttgcg cgcacgaacg catgcacatt ggcgtcgagc tgcacgagcg
      121 catcggtgag gtgcgagccc atgaagccgt cggcccctgt gaccagacag gtcctgccgg
      181 tgaagaagtc gccgaagtcc cgccggagct cagcgacggc atcgtccgtc caggtggagt
      241 cgtgttggtc tgctttcatg cagcggtgcc cgccgacgag cagcccaagg gtagcccggg
      301 gcgcggccgg agtgccgttc gaagtggcgc ctttacaggt ctctcatatg tgatcctgag
      361 cctggccgcc gcagttgccg gtgcctttct ggcgcacaag ttcgggagga acaaggagac
      421 cgacggcttc atggccgcct acggcgtcta cctcgtgctc gtgctcggcg cccaggcctt
      481 ccggatggtc gtcgttccag acctgactcg cgccgaggcg gaggggcggc tcggctcgga
      541 gtttcgggcg tacgcgcttg ccttcctggc cgtagccatt cctgccaccg tcgtcgcggc
      601 cctcttcgca catttctggg gtgagttgat caccggacgg cttccgaagg aatcggctgc
      661 cgttgccgcg gatgcactgc cgtggctcgt gcccgcggcc ttcgcgcagc tgatcgcggc
      721 gcttgccgcc agcgcgctgg ctgccaagga cagctacgtt ccggctgctc tcggtttcgc
      781 gctcggcggc gtcggcggcg tcgtcttctt cgtgctggcc gcggactctc acggtctggt
      841 tgcgcttgcg tggggactga cgctgaacgg cgcgatcgcc atcgccctgc cgatgatcgt
      901 cctgttcctc cgcgggaacc ggctacgagc gcatcgaggc gtcccgctcc ggctcggcca
      961 tcggctctgg cggctcttgt acggcgcagc cgtgccggtc gcccttcaag gcctctacgt
     1021 gatcgggctc cggtttgcgg ccgggaccgg tgagggcagc gtcacgagcc tgtcgtacgc
     1081 gtatctgctt gcagcgacgt tcgtcagcgc cacggccttt tcgctgtctc tcatctctgc
     1141 agcaccgttg acccgacgtg gtgtcaatcc cgagactgcg gcggaccacg tcgtgcactc
     1201 gacgtggatc tcgctcgctt tcgtcggcgc cgccgcgggg ctcgtagcgc tcgtcggcgg
     1261 gcgggtgatc actgcggtcc tcggccaagc attctcgggg cacgtcggtg cccagctcgg
     1321 gcggctcgtc atctttctct cgccgtggat ggtggcgaac gccgcgttcc tgatcatgta
     1381 tccgttgctg ttcgtcatgc accgcacacg gctgctgatc ccgctggcga tcgcggctgt
     1441 cgtcgtcgac attcccatct cgatcgccgg ccgcgccctc tggggtctca cgggcgtgac
     1501 ggtcgcgctc ggcgtctcga cgctccttgt cgtgctcgcc ctcatggcgt cgctcgcacc
     1561 tcgcatgctg gccctgaccg tcgtcgggct ggggcgtctc tcgatcctcg tcgcggcggc
     1621 gaccgcgctt gccttcggcg gcgcttcgct cgtcctgagc gcggtgcccg cggcagcggc
     1681 gggactggcg ctctatgcct tactgctggt ggcgatgcga cagctcggtc tggcccaggc
     1741 gtggcactac gtgcgtgccc tccactgaca ggcgcacccc gtacaatcat ccgaatgtcc
     1801 gggccggagg cgaaaaaccc ggcgcctgca ggtatttcct cgccggagga gatcgatgtc
     1861 gccgccctgt tcgatcggct ccgcgaggag cttcggcgtg gtgccgtcca cgaggactcg
     1921 cggggcgccg aatacgcctc gacacgggcg ctcgcggagc gtttctggcc ggttacggcc
     1981 gagcatccgg ccggcggcgg ccccaaggga gtggtcaagc gcttcctccg caagctcatg
     2041 cgctggtacg tcgaaccgct cgccgccgac cagcgtgttt tcaacgactc cgtgctgaag
     2101 ctcgtcgacg ccttgtcgga gcgggccgac tcttccacgg cggcacggga gcaggcggag
     2161 cggctcgtgc gggaattgga ggaacggctc gcccgtctcg aacgccgcgg cccggccacc
     2221 ggtggagcgc ccgcgccggc gacggtcgcg acgcaaccag ccgccgcgtc ggttccggac
     2281 tacttcggtt tcgaaagcag gatgcgcggg agcgtcgagt ccatccgcga gcgccaacga
     2341 cgctacgtcg acgatctgcg tcatgacgcg cccgtactag acatcggctg cgggcgtggc
     2401 gaattgctgg ggctcttgcg ggaggccggt gtggaagctc gtggcatcga tgccgacgcc
     2461 gacatggtcg cgtacgcgcg cggcgacggc ctcgaggtcg agcaggcaga cctgatcgac
     2521 tatctcgacc gagtaaccgc tggctcggtc ggagcgatct tcatgggcca ggtcgtcgag
     2581 cacctgccgg ctccgacgct cgtgcaagcc ctgcggctcg cggccgcgaa gcttcgaccc
     2641 ggcggcgttc tcatcgcaga gacgatcaat cctctctcac cgatcgcgct gcgcaactat
     2701 ttcgcggacc tgacgcacgc gcagccgctc gtgcccgaga ccctcgagct gctcgcccgc
     2761 caatcggggt tcgcgaagac cgagatccga tttctcaacg agcccgccga gcggctcacc
     2821 gagccggacg acccggtgat cgccgccaac gtccgccgcc tgaacgaact cctcttcggc
     2881 ccgctcgact acgcgctcgt ggcgcgaacg gctacgcatg agtagtgacg cggagacatt
     2941 ccgctcgtga cacggacacg tgactgcttt ttccttaacg tgtcggtggg cgggcgtggt
     3001 gggacccggt cagtcgcgcg tgtacctgta gaaggccgga gatgaggatc gccgtttgcg
     3061 caccgcaggt gccgttcgag cgcggcgggg ccgagatctt cgccgatgat ctggtcggcg
     3121 agcttcgcgc tcgcgggcac gagacggacc ttgtcacggt tccctacaag tggtatccgg
     3181 gcgagcgcgt gctctcgcag gccttcctgt ggcggctgct cgatctgagt gaatccgacg
     3241 gacggccgat cgagctggca atcgcgacga agtttccctc ctatgcagtc cgtcatccga
     3301 acaaggtggt ttggctgctg caccagttcc ggcaggccta cgagctcgat cgcaccgagc
     3361 tcgggcagtt cggtgagagc gcagaggagc gcgcgacgcg ccgcgccgtt cagcggctcg
     3421 atcgcgtggt tctgggtgag gcgaggaagg tgttcgcaac ctcccgcaac gtcgccgacc
     3481 gcctgcagcg ctcgacggga atcgccgcgg aggtgatgcc gcacccgccg caggagctgc
     3541 cgtacaggac cgagagctac gacaacttcg tgctctcggt gggccgtctg gaccgcgcaa
     3601 aacgacacga gctgctgctc gacgcgctgg ccaccgactc gacgctgcac tgcgtgatcg
     3661 tcggcgacgg tccggaccgc gagcggctcg aggacgttgc gcgcaggtac gggctcgacg
     3721 ggcgcgcacg cttcacggga cggatcgacg aagaagagct cgctgggctc tatgcacgct
     3781 gcctcgccgt gtactacgcg ccggtggatg aggacttcgg catggtcccc tacgaagcgt
     3841 tcctcgccga gaagccggtt gtcaccacga cggacgccgg cgggccgctc gaggtcgtga
     3901 ccgaccgggc gaccgggctc gtgatcgagc cacgtgccgc ggcgctggcc gaggcatgct
     3961 cctggctccg ggaccacgtc gacgacgcgc gggcgtgggg acaggcggga aaggagatcg
     4021 cgcgccgcgt gtcctgggac gaaacgatcg cgcgcctgct cgcgtgaggc ggtcagtggc
     4081 gtgagggttg cctactactc cccgctgccg ccagaacggt cggggatagc cgactactcc
     4141 gcgctgctgc tgcccgcact cgagcggctg gtcgagatcg cgatcgtgcg gcgcggacgc
     4201 acgcgacccg ttgccgccga cgttgcgctc taccacgtgg gcaacgatcc ggaagcgcac
     4261 ggctggattg tcgatgcgct gaagcgacgg ccgggggtgg tggtgctgca cgacttcgtc
     4321 ctccaccacc tcgtcgcagg gctgacgatc ggccggaagg atggccacgg ctatctcgct
     4381 gccatggagc gcgacgccgg cattcccgga cgtctgctcg cgcacggcgt tctcgacggt
     4441 cgtgtgtctc ccccttggga aacgcggccc gatgagttcc cactcgccgg cgaggtgctc
     4501 gccaacgcca ccggtctgat cgttcactcg cactatgtcg aggaccgcgt gctcgaggcc
     4561 ggctaccacg gtccgatctg gcggatcgag catcccgctt ggcccgcggc agaggttctc
     4621 cccgtgcaga tcgagggccg gccgctcttc ggctgcttcg gccatctcaa cgcgagcaaa
     4681 cgcgtccccc agttgatcga ggcgttcggc ttggtgcgaa agcggcatcc gagcgcgaag
     4741 ctcctgctgg tcggatccgc atcgccgggc ttcgacgcgg agcggttcgc gacggcaggc
     4801 atcgagcgcc tcggctacgt cgaagaacag cagctctggt cgctgatggc ggcgtgcgac
     4861 gcgtgcatct cgctgcgctc gcccacgatg ggagagac
//
LOCUS       DASVEU010000196         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_331386,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000196 DASVEU010000000
VERSION     DASVEU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331386"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1391
                     /locus_tag="VF225_08090"
     CDS             21..1391
                     /locus_tag="VF225_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX7145256.1"
                     /translation="MSIILINAVGLTPRLLPFAPRLHALAQAGWHVPLRDVVPAVTCS
                     AQATILTGKPVDQHGVVGNGWLFRDTMEVRLWQQSNRLVQAPKVWESMPAITTANLSW
                     WFAMYSTADVTVTPRPMYPADGRKIPDCYTKPQGLRDVLQAELGRFPLFKFWGPAASI
                     DSTRWLAEASKLVEMRFGPDLTLVYLPHLDYGLQRKGPAERDVRVELGELDGVAGDLI
                     DFYEDRGARVIVLSEYGIVPVSRAVHPNRVLREAGLISYRVELGREVLDVGASDAFAV
                     ADHQIAHVYVGDPRRVPEVRALLAAVPGVGAVLDEEGKREHGLDHERSGELVLVAESD
                     AWFTYYYWEDDARAPDYARTVDIHRKPGYDPVELFLDPAIRIPPLALGSRLLRKKLGF
                     RTLMDVIPLDASLVKGSHGRIPDDPSKGPLLMTRQTDLLDDDLAATGVHDLILRHLDA
                     RVVRRT"
     gene            1480..2970
                     /locus_tag="VF225_08095"
     CDS             1480..2970
                     /locus_tag="VF225_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7145257.1"
                     /translation="MRVVIGVVAVLGFALVAASTSLAQSSSLERGLLEAVQAVSFDHV
                     IDFGPVEDVCPSMSLCPSSAPSIATAPNVDVAVIELARNGRARRAANVILSRDYPAGL
                     VVPIDRNLGTSAVRFRGWDIDRWNGGTFASDGGQISTKGWNDSPPRTAADDIVAGRDR
                     APFEFMAPYPASLFKLIVAFHTLRLVDRGILGLETAYAYDPAGGCGAAAPGTETNHRW
                     LDHMITSSNNRAACALIKQLHGLGEVEGMNAELRELGLSTLQVNGTSALTGGTWQPGQ
                     IHMTALDTARLLWLIKGAPGVLWQRPDGRPVTARVLSDSSRALLGQLLAEQGFNVVLS
                     TTNFCGLKYPSRGIPQRIAERWIDPSDGSVTVEGMHYGRDVRPCNAAAEVSFAHKTGL
                     TYNYGSDAGVVRSLDGKPSRRYVIAFLSNLGYRYSDARFATSRQLPCFSLGFCYTEKI
                     AQLAKRVDELLRGQPLAGRQAPGSGGQTGDLRTGEARRRAIRRTGG"
     gene            complement(3145..3603)
                     /gene="grdA"
                     /locus_tag="VF225_08100"
     CDS             complement(3145..3603)
                     /gene="grdA"
                     /locus_tag="VF225_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085916664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3478..3480),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEX7145258.1"
                     /translation="MFESKQVIVLGERDGIAGPAIAACVKSAGGNVAFASTECFVUTA
                     SGAMDLNSQEVIKRLADEHGTDQLVVILGAPDAESAEIAAETVVLGDPTYAGALAEAQ
                     LGLSVYHALEEEFRSQVDDDVWEEQIGIMADVLEAEEISAAVKGMREQKP"
     gene            complement(3610..3951)
                     /locus_tag="VF225_08105"
     CDS             complement(3610..3951)
                     /locus_tag="VF225_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7145259.1"
                     /translation="MGAVSEATRENFDALIESGTTLVDVWGPECRPCLALKPHVEKLA
                     EARDDLTVVMLEAPKARRVCIRLKVHGLPTFLLMHDGEEVARLSASTISPTELKTWLD
                     RSLGEMREEVS"
     gene            complement(3956..>5088)
                     /gene="grdC"
                     /locus_tag="VF225_08110"
     CDS             complement(3956..>5088)
                     /gene="grdC"
                     /locus_tag="VF225_08110"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HEX7145260.1"
                     /translation="VDDFNLVKLGAQEAGAFAERLARHPLAEHLDLERVAAAGGDAEA
                     IAAEPGAVALHLGADRLAGAMRRAHESDAALTAEVLLENLAGKASATLALLRLLADNG
                     IDPDSIDYVIGSGEEAIGDRYQRGGGNLAKSVAAAAGCSNASGADVKNFCAAPVPALV
                     IASSLVASGVFRRVAVIAGGSLPKVGMKFQGHLKHGLPVLEDVLGACALLVEADDGRS
                     PRVRLDSVGVHPVAAGSSNPKIMEALSITPLDRLGMGMLDVDDYATELHNPEITEPQG
                     SGNVPERNYKTLAALAVRRGDLDRADMGAFVEQRGMPGFAPTQGHIASALCYVPHARA
                     RLIDGGARRVQLIAKGSLFLGRMSEQSDGMSVLLESNDEAGG"
BASE COUNT          772 a         1782 c         1697 g          837 t
ORIGIN      
        1 gtcgcggcta acctcgcagc atgtccataa tcctgatcaa cgcggtcggt cttactccgc
       61 gcctgttgcc tttcgcgccg cgattgcacg ccctcgcgca ggctggttgg catgtcccct
      121 tgcgtgatgt ggttcccgcc gtcacctgtt ccgctcaagc gacgattctc accgggaagc
      181 ccgttgatca gcatggcgtc gtcggcaacg gctggctctt ccgcgacacg atggaggtgc
      241 gcctgtggca gcagtccaac cgcctcgtgc aggcgccgaa ggtgtgggag tcgatgcccg
      301 ccatcacgac ggcgaacctg tcgtggtggt tcgctatgta ctcgacggca gacgtgacgg
      361 tcacgccgcg gccgatgtac cccgcggatg gtcgcaagat ccccgactgc tatacgaagc
      421 cccaaggcct gcgcgacgtc ctgcaagctg agctcggccg gtttccgctg ttcaagttct
      481 ggggtcccgc ggcttcgatc gattcgacgc gttggctcgc cgaggcgtca aagctcgtcg
      541 agatgcgttt cgggcccgac ctgacgctcg tctatctgcc gcacctcgac tacggcctgc
      601 agcgcaaggg accagccgag cgcgacgttc gcgtcgaact cggcgagctg gacggcgtcg
      661 ccggcgacct gatcgacttc tacgaggatc gcggcgcgcg cgtgatcgtg ctgtcggagt
      721 acggcatcgt gcccgtctcg cgagccgtgc accccaaccg ggtgctccgc gaggctggtc
      781 tcatcagcta ccgggtcgag ctcggccgcg aggtgctcga cgtcggcgcc agcgacgcgt
      841 tcgcggtggc cgatcatcag atcgcgcacg tctatgtcgg tgacccccgg cgcgtgcccg
      901 aggtccgggc gctgctcgcc gccgtaccgg gcgtgggggc ggtgctggac gaggagggca
      961 agcgcgagca cgggctcgac cacgagcgtt cgggcgagct tgtcctcgtc gccgagtccg
     1021 atgcctggtt cacgtactac tactgggaag acgacgcgcg tgcgcccgac tacgcgcgta
     1081 cggtggacat ccaccgcaag cccggctacg accccgtgga gctcttcctg gatcccgcga
     1141 tccggatccc gcccctcgcg ctcgggtcgc gcctcttgcg aaagaagctg gggttccgga
     1201 cgctcatgga cgtcatcccg ctcgacgcgt cgctcgtcaa gggctcgcac gggcgcatcc
     1261 cggacgatcc atcgaaaggg ccgctgctga tgacacgcca gacggacctc ctggacgacg
     1321 atctcgccgc gaccggagtg cacgacctca tcctgcgcca cctcgatgcc cgggttgtgc
     1381 gccggaccta gactctctcg agcctgggaa gccgggagat cggatcacgg agtgacgctc
     1441 acacctgtgc gcaaggggac tggaagtatg aaccggcgct tgagggtcgt catcggagtc
     1501 gtcgccgtgc ttggctttgc acttgtcgct gcctcgacgt cgcttgcgca gagcagttcg
     1561 ctcgagcgcg gcttgctgga ggcggtgcag gcggtcagct ttgaccacgt catagatttc
     1621 ggcccggtcg aagatgtgtg tccgtccatg agcttgtgcc cgtcttcggc gccgagcatc
     1681 gcaaccgcgc cgaacgtgga tgtggccgtc atcgagctgg cacgcaacgg ccgcgcgcgg
     1741 cgggcggcga acgtcatcct ctcgcgggac tatccggcgg gtctcgtggt cccgatcgat
     1801 cggaacctcg gtacgagcgc cgtgcgcttc cgcggctggg acatcgaccg ctggaacggc
     1861 gggacgtttg ccagcgacgg cggtcagatc tcgaccaagg gctggaacga cagcccgccg
     1921 cgcacagccg cggacgacat cgttgccggc cgcgaccgag ccccgttcga gttcatggcg
     1981 ccctacccgg cttcgctctt caagctgatc gtcgcctttc atacgctgcg gctcgtcgat
     2041 cgagggatcc tcggcctcga aaccgcctac gcctatgacc cagcgggagg ctgtggcgcc
     2101 gcggcccctg ggaccgagac caaccatcgc tggcttgacc acatgatcac ctcctccaac
     2161 aaccgcgctg cgtgcgcgct gatcaagcag ctgcacggcc tcggcgaggt cgagggcatg
     2221 aacgccgagc ttcgcgagct cgggctcagc acgctgcagg tcaacggcac gtcggcgttg
     2281 accggaggga cctggcaacc gggccagatc cacatgacgg cgcttgacac ggcgcggctc
     2341 ctgtggctca tcaagggagc gcccggcgtg ctctggcagc ggccggacgg ccgtccggtc
     2401 accgcgcggg tgctatccga ttcctcgcgc gccctgctcg ggcaactgct cgcagaacag
     2461 ggcttcaacg tggtcttgtc gaccacgaac ttctgcggcc taaaatatcc gagccgaggg
     2521 atcccgcagc gcatcgccga gcgctggatc gatcccagcg acggcagcgt gacggtcgag
     2581 ggcatgcact acggccggga cgtccgtccg tgcaacgctg cggccgaggt gagcttcgcg
     2641 cacaagaccg gtctgacgta caactacggc tcggacgccg gggtcgtgcg gtcgctcgat
     2701 ggcaagccga gccggcgcta cgtgatcgcg tttctctcga acctcggcta tcgctacagc
     2761 gacgcgaggt ttgccacctc acgccagctg ccatgcttca gcctgggctt ctgttacacg
     2821 gagaagatcg ctcagctcgc caagcgagtc gacgagctgc tacgcggaca gcctctggcg
     2881 gggcggcaag ccccagggtc ggggggacag acaggcgacc tacgcaccgg cgaagcccgc
     2941 cgccgagcga tacggcgaac cggcggctga gccgtcagcg cccagtcgca agacgaccgc
     3001 acggtgcggc gccgtgcacg tcgcttcgcg acgaggtctc agctcagtga actgaccgtg
     3061 gaggcgggga ggtggactgc aaaccgccgc ctcaagaacc ggcgcagcgg ctcagccctc
     3121 agtgcaggct gcgcccatgt cgcgctacgg cttctgctcg cgcatgccct tgaccgccgc
     3181 ggagatctcc tcggcttcga gcacgtcggc catgatgccg atctgctctt cccagacgtc
     3241 gtcgtcgacc tggctgcgga actcctcttc gagcgcgtga tacacggaga gacccaactg
     3301 ggcctcggcc agggcgcccg cataggtcgg atcgccgagc acaacggtct cggcggcgat
     3361 ctccgcgctc tccgcgtctg gagctccgag gatgacgacc agctggtcgg tcccgtgctc
     3421 gtcagcgagg cgcttgatca cttcctggct attcaggtcc atggcccccg aggcagttca
     3481 gacgaagcac tccgtcgacg cgaaggcgac gtttccgccc gcgctcttga cgcacgcggc
     3541 gatcgcggga cccgcgatcc cgtcgcgctc tccgagcacg atgacctgct tgctctcgaa
     3601 catagggtgc tagctcacct cctctctcat ctcgcccagg ctcctgtcga gccaggtctt
     3661 gagctccgtc gggctgatcg tcgacgccga caaccgcgcg acctcttcgc cgtcgtgcat
     3721 gagcaggaag gtcggcagcc cgtggacctt gaggcggatg cacacccggc gggccttggg
     3781 cgcctcgagc atgacgaccg tcaggtcgtc gcgggcctcg gccagcttct cgacgtgggg
     3841 cttcagcgcc aggcacggcc ggcactccgg cccccacacg tcgacgagcg tggtaccgct
     3901 ctcgatgaga gcgtcgaagt tctcgcgcgt cgcctcgctc actgctccca tccgctcatc
     3961 ctcccgcttc gtcgttggac tcgagcaaca cgctcatgcc gtcggactgc tcggacatcc
     4021 gacccaagaa caggctgccc ttggcgatca gctgcacgcg ccgcgcgccc ccatcgatca
     4081 gccgggcgcg ggcgtgcgga acgtagcaca gcgccgatgc gatatggcct tgcgtgggag
     4141 cgaagccggg catcccccgc tgctcgacga aagcgcccat gtccgcccgg tccaggtcgc
     4201 cccggcgcac ggccagggcg gcgagcgtct tgtagttgcg ctcgggtacg ttgcccgagc
     4261 cctggggctc ggtgatctcg gggttgtgca gctcggtggc gtagtcgtcg acgtcgagca
     4321 tccccatgcc gagccggtcg agaggagtga tcgagagggc ctccatgatc ttcgggttcg
     4381 acgacccggc ggccaccgga tgtacgccca cggagtccag ccgcacgcgc ggcgagcgac
     4441 cgtcgtcggc ctccacgagc agcgcacacg cgccgagcac gtcctccagc accggcaggc
     4501 cgtgcttgag atggccctgg aacttcatcc ccaccttcgg gagcgaaccg cccgcgatca
     4561 ccgcgacgcg ccggaagacc ccggaggcga cgaggctgct cgcgatgacc agcgcgggga
     4621 ccggggcggc gcagaagttc ttgacgtccg cgcccgaagc gttgctgcag cccgccgctg
     4681 ccgcaaccga cttggcgagg ttgcccccgc cgcgctggta gcggtcaccg atcgcctcct
     4741 cgccggagcc gatgacgtag tcgatcgagt ccgggtcgat gccgttgtcg gcgagcaggc
     4801 gcaggagcgc cagcgtggcg ctcgccttcc ccgcgaggtt ctcgagcagc acctccgcag
     4861 tcagggcggc atcgctctcg tgggcgcgac gcatcgcccc ggccaggcga tcggcgccca
     4921 ggtgcagcgc cacggccccc ggctccgccg cgatcgcttc ggcgtcgccg ccggcggccg
     4981 ccacgcgctc gaggtcgagg tgctcggcga gcgggtggcg ggcgagtcgt tcggcgaatg
     5041 cgccggcctc ctgcgcaccg agcttcacga ggttgaagtc gtcgaccg
//
LOCUS       DASVEU010000197        16719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1494790,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000197 DASVEU010000000
VERSION     DASVEU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16719
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494790"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF225_08115"
     CDS             complement(<1..347)
                     /locus_tag="VF225_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX7145261.1"
                     /translation="MIVVSDEVREADAVVALETTLVAHGFPAGDGLAVGLEAEQRVRA
                     AGAVPATIGVLDGKIRVGLSAEELGRFDASARKAGPRDLAACVVQRAVGATTVGGTLA
                     VCRAVGIRFMGTGG"
     gene            complement(344..1156)
                     /locus_tag="VF225_08120"
     CDS             complement(344..1156)
                     /locus_tag="VF225_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7145262.1"
                     /translation="MLVVTLGDALLDVIVRLGEPLTSGADALATTQASAGGQAANVAA
                     WVVALGSQARTVSKRGDDLAGQLVTGELEGRGVELVGPVEGRNGIVVSLVDADGERTM
                     ASDRGGAPNLSAADLDPAWFACDCLHISGYALLAAPIDGAALHAATLARVQGARVSVD
                     LSAWTRIREHGAVRFRGQLEQLEPDVIFANEAEWEIVGGAYGLAPTAVVKRGARGLLV
                     LGEERVELPARGVDVVDATGAGDALAAGFLVGGAELALEAAARCVAQSGAMP"
     gene            1172..1375
                     /locus_tag="VF225_08125"
     CDS             1172..1375
                     /locus_tag="VF225_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145263.1"
                     /translation="MDGEHRGGRLGSFVVGGVLGASAALAAARRRRRRIEPQRPAFQG
                     LEAFEGAPCYREVVEKEREELQP"
     gene            complement(1364..2971)
                     /locus_tag="VF225_08130"
     CDS             complement(1364..2971)
                     /locus_tag="VF225_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7145264.1"
                     /translation="MIEVATDPSPLARARTRVRDVDALWAGTTAVAATLAGVLGWFLR
                     TWPPHEDEALALFVGRGSLGHVLHTVVAERGGAPLHFGLAWLVVHLGGGLTGLRLVSL
                     LFAVASVPLIALLGARLADRTAGLVAAALASGSWVLMFHGIYGRMYSLFLFTSLLSFL
                     ALLSALDRGGRRRFALWGLALLLMLASHPYAALVVAAQGLFVVLRRRRLRSAILTLAA
                     VGVAGIPFWWADLVLRDRFDVGVGGGGPELGSPTSVLHYFWWVAGDFSAGHRAWSTPV
                     LVLALVGTILLWRRRPESVGLFACVVAVPGLAFMLATLNSTASPEARHLIFALPFFSV
                     LLAVPLVEAGRRGGRLGPAVAAIAVVVLLIGEVRWTHEKTPPIFDGDPPGEAQARDAA
                     GAWLASTYRPSDVLLGYEPVYLQAWEQNRSFSGHTLPRADPALLASALKEIPEPLGRG
                     VWVFDASDTTNVKERQTIRYALPTPQAAFEARVYGPFLVIRSRKPLGTRANYVAVSEE
                     VMRLGRTLEIGDADINLRALLRAESRL"
     gene            3026..3328
                     /locus_tag="VF225_08135"
     CDS             3026..3328
                     /locus_tag="VF225_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7145265.1"
                     /translation="MPTYEYKCPNGHVFEVFKRIADPPPEACPVCGASPVETVLYPVP
                     VHFRGSGFYSTDYGRGGRKRDRAKESSAESGSDSGSGSGDKKDKKDTPAEKKAAEG"
     gene            complement(3329..4501)
                     /locus_tag="VF225_08140"
     CDS             complement(3329..4501)
                     /locus_tag="VF225_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7145266.1"
                     /translation="MPTEEKPYRVYRGGRVKGKVPLERPQQERPDRNGRGPREPKIRR
                     PRRRWSWKRRLGLGLIVLLVVAVVWGIAGYLSFRSGVDKANARLDQSVLPVLSKQNGL
                     MLSNPTTMLLLGRDHENTDRRASLNHSDSIMVLRTDPSRHRLVYLSIPRDLRVPIPGH
                     GEDRINSAFQLGGAALTVRTIESLTGLQINHVMIVDFRSFKSLIDNIGGVDINVPAPI
                     LSNKFDCPYKASRCANWHGYRFNKGMQHMNGQRALIYSRIRENQLNPADSDITRGLRQ
                     QAVMRAVMAKLLGFGTVVSLPFNGDSLLKPLTTDLSAWQFVQLGWVLKRAGQKNALHC
                     RLGGTATSIGGASVIVGDEQNISVVQMVTGRAAPQPPQPGSGQYGPGCVVGNRQFK"
     gene            complement(4580..5215)
                     /locus_tag="VF225_08145"
     CDS             complement(4580..5215)
                     /locus_tag="VF225_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145267.1"
                     /translation="MSSDKERSSMKPPLTSVALVVVALAAAGLAADRIFSAGTASATP
                     GVGFSTTILARGIFGENFTFGTPTQITVKRKVSIKTKSGVVRKTVKFKVDSVLRAITC
                     SPTNPCDTAFQQARVEPGGSSGWHTHPGATFVAVVEGEGTLYRVVGSDCTATKLVAGA
                     GFAQMPTERHVVRNEGTVPFVVDTLYVLPPGTPNTGIRVDQPQPAECPLIH"
     gene            5336..5770
                     /locus_tag="VF225_08150"
     CDS             5336..5770
                     /locus_tag="VF225_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7145268.1"
                     /translation="MNQLLSGILDEKGHDVLQIDADASVFEAVKRMVEANVGALLVTV
                     SGEITGIVTERDYLRRAALEGRPDKETPVREIMSSPLLVATPETSVDECMALMTDRRI
                     RHVPVVDGGEVVGIVSIGDVVRFKSKQQSFELQFLHDYITAS"
     gene            5858..8062
                     /locus_tag="VF225_08155"
     CDS             5858..8062
                     /locus_tag="VF225_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145269.1"
                     /translation="MKEALQMRSFTRFSSRSRRGLRFLAAVCLLAGAALALSSTASAA
                     SIVSAGPLTSITISPDLNCNVLHTGDTFPEWYQGTACGTLAVDQSTATLYGPLSIPAG
                     GAAVPRTAFTPISQVGPTGTGTSLDPFKIVTVAALGLSGLTITQTDSYVVGQESYRTD
                     VQISNSNLTSRSVRLYAAGDCYLQNSDYGYGRVDGSAIACTATSDPGSRIEQLFPLTS
                     GSSYMEDNYNTVWAQIGARAAGPNTCICATLDDNGILLSWDVTVPGAGSATVSHLTTF
                     SPLGFAPLSTTKTADSSTAAAGGPDGYTITIHNPNASPVVVNSVTDTLPAGFAYVAGS
                     TTGATTSDPTISAQTLTWSGSFSAPAGGDVTLHFNVTASSTPGTYYNNAAGDAGAVVV
                     AATGDTAPITVAAGDEPITATGTTFSATEGASFSGEVATFTDPDTAATASEYSATIDW
                     GDSSATTAGTIGGSAGSFSVSGTHTYTEEGSYSVTVTIADVDDAANTDTANSTAIVAD
                     AALHAGTFTVPPSTSLGNPTNVSFSFSDDNTGAPTSDFTATIDWGDASNSTGVIGGSG
                     GNYTAGGSHTYAAAGPYTITVTVVDDGGSTTGATAATSVVVVPPAAALSAGYWKNHQE
                     HTTSLLSIALGGYTVGTFADATAVFANMNCGKSSANDAAGCLAAQLLAAKLNLKNGAG
                     TCIAPTIASADAFLVSIGYTGPNATYTLTAAQRAQAIELKDVLDKYNNGLGC"
     gene            8136..9029
                     /locus_tag="VF225_08160"
     CDS             8136..9029
                     /locus_tag="VF225_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7145270.1"
                     /translation="MNEKRVIGVDIGGTKILAGVVDRDGHVEHRREHATPTASEDAFF
                     AGLDEAVEELLTDEIAALGFGIPSPIDQRTGRALQAVNLPLTDIAFRDRMKERFGLPV
                     GIENDANAATYAEFRFGAAREVDSMVMLTLGTGCGGGAVVDGQLFRGWAEFGHIVIVY
                     DGLPCQGSCTGRGHLEPYVTGVAATKLAQAEFGPAVDAHRLVRLANEGEPRAVEILDG
                     IGRHLGAGIGSLVNIFNPDLVVIGGGFAAAGDFVLDPAREILRREALARAGYRVPIER
                     AELGTAAGLIGAGLVAFDVAG"
     gene            9034..9837
                     /gene="rsmI"
                     /locus_tag="VF225_08165"
     CDS             9034..9837
                     /gene="rsmI"
                     /locus_tag="VF225_08165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7145271.1"
                     /translation="MPLAVCATPIGNLEDVTLRLLDELRSADVILCEDTRHTRVLLDR
                     HGISGAKLLSYHEHNEAKRTAELLPRLLAGDRVALVSDAGMPGISDPGARLVGAALEQ
                     GVPVTVLPGPSAVDTALVAGGFAADRYQFVGFLPRGAGALTALWRELAGWAHPVVAFE
                     SPQRLPGTLRSLAAVQPGREVAVCRELTKKFEEVVRGSAAEVCARFVEPPKGEITLVL
                     GPGEDERDLPAALEAVAELVAAGVPRRQAAEVVSRLTGASRNELYSRTL"
     gene            10084..10260
                     /locus_tag="VF225_08170"
                     /pseudo
     CDS             10084..10260
                     /locus_tag="VF225_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
     gene            complement(10293..10625)
                     /locus_tag="VF225_08175"
     CDS             complement(10293..10625)
                     /locus_tag="VF225_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145272.1"
                     /translation="MDSGAATSGATRELAGRSVVAWPLAIVGWIAASSCGDTAGLSAV
                     LRSELPLGSLAGDGPAVAADAASRVSRGTATTAGSAAGEGAGASREGSMVSGSTYPCG
                     SLVMRVPK"
     gene            10949..12472
                     /gene="metG"
                     /locus_tag="VF225_08180"
     CDS             10949..12472
                     /gene="metG"
                     /locus_tag="VF225_08180"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7145273.1"
                     /translation="MNSFYITTPIYYVNSTPHIGHAYTTIVADIIARHHKQRGDETFF
                     LTGTDEHGSKVYRAAEEQGLDPKTYVDQIADAWKELPRRVDADYDFFIRTTDEGHKRF
                     VQEFIQRIYDNGYIYQDVYSGLYCVGCEEFKAEAELVDGKCPIHGTVPERIEETNYFF
                     KLSAFQQPLLDLYDARPDFVLPRFRYNEARSFIEGGLQDFSLSRAGQPWGVPVPWDES
                     QVVYVWVDALINYVSALTYAREGEDLREKFWPEVRHLLAKDILRFHCVFWPALLLAAG
                     YEVPKQLFVHGWLLVDERKISKSVGNVIDPLDLVDVYGSDAVRFWAVRAVSFGQDGNV
                     TLESLHERYESELANDLGNLVSRTTAMIARYREGRIPAATGESKVSDAIEAVKREVPA
                     RLDLFDVTGAVDVIWDLVRSLNKHVEQTKPWELAKDDAHAGELDRVLYDLADGLRVAA
                     IALSAYLPQTAPRILQALGQPEDLAWKNVEAGRTVTAAGIEPATPLFPRVDAPTAAA"
     gene            12469..13209
                     /locus_tag="VF225_08185"
     CDS             12469..13209
                     /locus_tag="VF225_08185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7145274.1"
                     /translation="MIDTHAHLEALDEPSAAIARARDAGVARIVTIGTDPISWAKMLP
                     IVDEHDGVYGVAGIHPHAAASEFDFSALERLLAHPKVVAVGEIGLDYYRDYAPRDAQT
                     KVFIYQLGLAKRAQLPVVIHNRAADDDTVSILRQWFDGTVVLHCFSSAALLPEAVERG
                     WYVSFAGNVTYANAGDLRDAARAVPAELILAETDCPYLAPQPVRGKTNEPAYVVHTVA
                     ALAEARGEESDALAAQIDANATAAFGLP"
     gene            13289..14065
                     /gene="rsmA"
                     /locus_tag="VF225_08190"
     CDS             13289..14065
                     /gene="rsmA"
                     /locus_tag="VF225_08190"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7145275.1"
                     /translation="MSISPKRRLGQHFLVDENILGVIGRLAQLDEDDVVLEIGPGLGI
                     LTTYLADRVSQVYAVELDASLGPQLIERLAGRPNVELRFGDAVKLDLAEVAPGAKKLV
                     ANLPYNVATPLVAESLDHLPGIELWTVMVQREVADRFFAVPSTKAYGAVSVLVQLVAE
                     RTGFHPVSATVFRPRPNVDSALVAFRRTGLPHDFRRVKRVVEGSFAHRRKRLANSLEL
                     AGVAERGRVEAAIATIGREPNVRAEELAPHEFLALTEALK"
     gene            14062..14874
                     /locus_tag="VF225_08195"
     CDS             14062..14874
                     /locus_tag="VF225_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145276.1"
                     /translation="MTRAPAAAKINLSLVVGPVRDDGKHEVVTVYQRVALSDRIAVER
                     SSELGVAGFAGDTLVRRALECMVDGDGASFTARIQKRIPVAAGLGGGSSDAATALRLA
                     NGLRTDPYPAERLRIFARTLGTDVPYFLEDGPQLGTGDGTDLEALELPQDYWVLLVLP
                     RKALKSSTEHVYAEFDARGGEHGYGERRAALLGALERVKRPRDLATLPSNDLASSPLA
                     RELVGLGAFRADVTGAGPTVYGLFLHGHQARAAQRQISPKGRTWLTAPAWYG"
     gene            14879..15247
                     /locus_tag="VF225_08200"
     CDS             14879..15247
                     /locus_tag="VF225_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145277.1"
                     /translation="MAYSQPMIEGGSTRIGRWLRERRLRLALWVAVVEGLLVALTPDL
                     TKWTVLVIGAILLAFYIVAGRSMRWDVARQLSWIAAASQALAILVVILAFVLKLVAIV
                     AVVVFALIALAYLFSDSRRT"
     gene            complement(15339..15734)
                     /locus_tag="VF225_08205"
     CDS             complement(15339..15734)
                     /locus_tag="VF225_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145278.1"
                     /translation="MKHAIRSLLVVAATLAAVLAITAAPSSAGVTPDQLTQAGWTCVQ
                     PLADPTRRICAPPGLGLPPLPGTPGFADRGPSYELLVFAFATGEFIGVQHLLRPDIYL
                     RGTPPCPQQPNGQYIYIPRNDTWSCFRAK"
     gene            15889..15959
                     /locus_tag="VF225_08210"
     tRNA            15889..15959
                     /locus_tag="VF225_08210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15921..15923,aa:Gln,seq:ttg)
     gene            15965..>16719
                     /locus_tag="VF225_08215"
     CDS             15965..>16719
                     /locus_tag="VF225_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX7145279.1"
                     /translation="MRSELAAVVMAAGLGTRMKSATPKHLHPLLGRRLVDWVVEAVRP
                     LGADPLVVVLSPEAANALDGVTVAVQQRPLGTGDSLRAARESVGDAETVLVVSGDHPR
                     ISSDLLQKLVTEHRSAGASATVLSFEPPDALAYGRIVRSADGRLKAIVEDADANDAER
                     AIREVNSSIYVFAADKLWPVLDRIEVANAQGELYLTDAVRLLVERGEEVVVFKAPDAD
                     EVEGVNTRVELADAAAFLRARVNREHMLAGVTIV"
BASE COUNT         2760 a         5639 c         5670 g         2650 t
ORIGIN      
        1 ccgcccgtcc ccatgaagcg aatccctacg gcgcggcaga cggcgagcgt gccgccgacg
       61 gtcgtcgcgc cgacagctcg ctggaccacg caggcggcga ggtcgcgcgg gccggccttg
      121 cgcgcactcg cgtcgaaccg cccgagctct tccgccgaga ggccgacgcg gatcttcccg
      181 tcgagcacgc cgatcgtcgc cggcaccgcg ccggctgcgc gaacccgctg ctcggcctcg
      241 agcccgaccg ccaggccgtc acccgcagga aagccgtgcg cgacgagtgt cgtttcgagc
      301 gcgacgacgg catccgcctc ccgcacttcg tcggagacga cgatcacggc atcgcccccg
      361 actgcgcaac gcaacgggcc gcagcctcga gcgcaagctc cgcgccaccg accaggaaac
      421 cagcggccaa ggcgtcgccg gcaccggtcg cgtcgacgac atcgacgccg cgtgcgggaa
      481 gctcgacacg ctcctcgccc aggacgagca ggccgcgggc gccccgtttc acgaccgctg
      541 tcggcgcgag cccgtacgcc ccgccgacga tctcccactc cgcctcgttc gcgaagatca
      601 cgtcgggctc gagctgctcg agctgtcctc ggaagcgcac agcgccgtgc tcgcgaatgc
      661 gagtccacgc ggagagatcg acgctcacgc gtgcaccctg gacccgcgcc aacgtcgcgg
      721 cgtggagcgc ggcgccgtcg atcggcgcgg ccagcaatgc gtacccggag atgtggagac
      781 agtcgcacgc gaaccacgcg gggtcgaggt cggccgcaga gaggttcggt gcgccaccac
      841 gatctgacgc catcgttcgc tcaccgtccg cgtccacgag ggagacgacg atgccgtttc
      901 gcccctcgac cggtccgacg agctcgacgc cgcgtccctc gagctccccc gtcacgagtt
      961 ggccggcaag gtcatcgcca cgcttcgaga cggtccgcgc ctgggagccg agcgccacga
     1021 cccaggcagc gacgttcgcc gcctgcccgc cggcggacgc ctgcgtggtc gctaacgcgt
     1081 ctgctcctga ggtgagcggc tcccccagac gcacgatcac gtcgagcagc gcgtcaccga
     1141 gggttacgac cagcacacct acaatcgtcc catggacggc gagcacaggg gtggccggct
     1201 cggctcgttc gtcgtgggcg gggtgctcgg cgcttccgca gccctggccg cggcacgtcg
     1261 caggcggcgc cggatcgagc cacagcggcc ggccttccag gggctcgagg cattcgaggg
     1321 cgcgccgtgc taccgcgagg tcgtggagaa ggaacgggag gagttacagc cgtgactccg
     1381 cgcgcagcag cgcgcgcagg ttgatgtccg cgtcgccgat ctcgagcgtc cggccgagcc
     1441 gcatcacttc ctccgaaacc gcgacgtagt tcgcacgcgt ccccagcggt ttgcgcgagc
     1501 ggatcacgag gaacggcccg tagacgcgag cttcgaacgc ggcctgcgga gtcggtaacg
     1561 cgtaccgaat cgtctgccgc tccttcacgt tcgtggtgtc gctcgcgtcg aagacccaga
     1621 cgccgcgacc gagcggttcc ggaatctcct tcaatgccga agcaagcaag gccggatcgg
     1681 ctcgcggaag cgtgtggccc gagaacgagc gattctgctc ccatgcctgc aggtacaccg
     1741 gctcgtaacc gagcagtacg tcgctcggcc gatacgtcga cgcgagccac gcccctgcag
     1801 cgtcacgcgc ctgcgcctcg ccgggcggat cgccgtcgaa gatcggcggc gtcttctcgt
     1861 gcgtccagcg cacctctccg atcaggagca cgacaactgc gatcgcagca acggccggcc
     1921 ccaggcgtcc cccgcgccgc ccggcctcga ccaggggcac ggcgagcagc acgctgaaga
     1981 acggcagcgc gaagatcaga tgacgcgctt cgggcgacgc agtcgagttg agcgtcgcaa
     2041 gcatgaacgc caggcccggc acggcgacga cgcacgcgaa cagcccgacg ctttcggggc
     2101 gtcggcgcca gagcaggatc gtgcccacga gcgcgagcac gaggacgggc gtcgaccagg
     2161 cgcgatggcc ggcgctgaaa tcgccggcaa cccaccagaa gtagtgcagc accgaggtcg
     2221 gtgacccgag ctccggcccg ccgccgccga cgccgacgtc gaagcggtcg cgcaacacga
     2281 gatccgccca ccagaacggg atcccggcga ctcccaccgc agccagcgtc aggatcgccg
     2341 agcgcagtcg cctgcgccgg aggacgacga acagtccctg cgcggcaacg acgagcgctg
     2401 cgtagggatg gctcgcgagc atgaggagca gcgccagtcc ccaaagggcg aaccggcgcc
     2461 ggccgccacg gtcgagcgcg gacaagagcg cgagaaacga aagcaggctc gtgaacagaa
     2521 acaggctgta catccgtccg tagatcccgt ggaacatgag cacccagctc cccgaggcga
     2581 gcgcggcggc gacgagcccg gcggttcggt cggcgagtcg cgcaccgaga agtgcgatca
     2641 gcgggacact cgcgaccgcg aagagcagtg agaccaggcg gagcccggtc agccctccgc
     2701 cgaggtgcac gaccagccat gcgaggccga agtgaagtgg ggcgccgccg cgttccgcga
     2761 cgaccgtgtg cagcacatgg ccgagcgagc cgcgaccgac gaagagcgcg agcgcctcgt
     2821 cctcgtgcgg cggccaggtg cgcagaaacc agccgaggac cccggccagt gtcgctgcca
     2881 ccgcggtcgt gccggcccaa agggcgtcga cgtcacggac gcgcgtgcgg gcgcgcgcga
     2941 gcggggaggg gtcggtcgcg acctcgatca tgggctttgt tttgaggctg tccaagccga
     3001 taacactgct agcgtcctaa atctcgtgcc cacgtacgaa tacaagtgtc cgaacgggca
     3061 cgttttcgaa gtcttcaagc gcatcgccga cccgccccct gaggcgtgtc cggtctgcgg
     3121 ggcgtcgccc gtcgagacag tgctctatcc cgtcccagtc catttccggg gctccgggtt
     3181 ctactcgacc gactacgggc gcggcggtcg caagagggac cgggcaaagg agtcctcagc
     3241 ggagtccgga tcggactctg ggtctggctc gggcgacaag aaggacaaga aggacacgcc
     3301 ggccgagaag aaagccgccg agggctgact acttgaactg ccggttgccg acgacgcagc
     3361 cgggtccgta ctgacccgat cccggctggg gcggttgggg ggctgcccgg ccggtgacca
     3421 tctgcacgac ggagatgttc tgctcgtctc cgacgatcac cgaggcccca ccgatcgagg
     3481 tcgcggtccc gccgaggcgg cagtgcagcg cgttcttctg gccggcgcgc ttcagcaccc
     3541 agccgagctg gacgaactgc caggcggaga ggtcggtggt caaaggcttc aggagggagt
     3601 cgccgttgaa cggcaggctc acgacagtcc cgaagccgag caacttggcc atcacggcac
     3661 gcatcaccgc ctgctgacgc aggccgcgtg tgatgtcgct gtccgccgga ttgagctggt
     3721 tctcgcggat gcgtgaatag atgagcgcgc gctgcccgtt catgtgctgc atgcctttgt
     3781 tgaaccgata gccgtgccag ttcgcgcagc gtgacgcctt gtaagggcaa tcgaacttgt
     3841 tcgacaggat cggcgccggc acgttgatgt cgacgccacc gatgttgtcg atcaggctct
     3901 tgaagctgcg gaagtcgacg atcatcacgt gattgatctg gagccctgtg aggctctcga
     3961 tcgtccggac ggtgagcgcg gcgccgccca actggaatgc cgagttgatc cggtcctcgc
     4021 cgtgtcccgg aatcggcacg cgcagatccc gcggaatcga gagatagacg agccggtgcc
     4081 ggctcggatc cgtgcgcagg accatgatcg agtccgaatg gttcaggctg gcccgccgat
     4141 cggtgttctc gtggtcgcgg cctagcaaca gcatcgtcgt cgggttcgac agcatgagcc
     4201 cgttctgctt cgagagcacg ggaagcacgg actggtcgag ccgggcgttt gccttgtcga
     4261 ccccgctgcg gaaggacagg tagcccgcga ttccccagac caccgcgacg actagcagga
     4321 cgatcaggcc gaggccgagg cgccgtttcc aactccagcg gcgacggggt cggcgaatct
     4381 tcggctcgcg cgggccgcgc ccgttccggt ccggccgctc ctgctgcgga cgttccagcg
     4441 ggaccttgcc cttgacacgc ccgccccggt agacgcggta gggcttctcc tccgtcggca
     4501 tcgagccgga agggtaactt caggccaaga tcgcgcgacg ctgagcggtt cttccctgca
     4561 gcctcggctt ttcgagccct cagtgaatca aggggcactc cgccggctgg ggctggtcga
     4621 ctctgattcc cgtgttcggc gtcccgggcg ggaggacgta gagcgtgtcc acgacgaatg
     4681 gcaccgttcc ctcgttccgg acgacgtgac gctcggtcgg catctgagca aagcccgctc
     4741 cggcgacaag cttcgtggcc gtgcagtccg agccgacgac gcgatacagc gtgccttcgc
     4801 cctcaactac ggcgacgaaa gtcgcccctg gatgcgtatg ccaaccagac gacccgcccg
     4861 gctcaaccct cgcctgctga aacgccgtgt cgcacgggtt cgtcggagag caggtgatgg
     4921 ctctcaggac ggagtcaacc ttgaacttga ccgtcttcct gacaacacca ctcttcgtct
     4981 tgatgctgac cttccgcttc accgtgattt gcgtgggcgt tccaaaggtg aagttctcgc
     5041 cgaagatgcc ccgggcgagg atcgtggtcg agaaccccac tcctggagtc gccgaggcag
     5101 tcccggcgga gaagatcctg tcggccgcca atcctgccgc cgccaaggcg actacgacga
     5161 gcgcaactga ggtgagcggt ggcttcatcg acgacctctc cttgtcgctg gacatggatc
     5221 ccgattgtct caaccgctgt ccttgccgac aaggttgcga aaagtcggcc tatgttcgac
     5281 gttttcgcgg cgctagagtc cccagagtcg gatcgcgacg ctgagaggag gcgcgatgaa
     5341 ccaattgttg tcggggatcc ttgacgagaa gggtcacgat gtgcttcaga tcgacgcgga
     5401 cgcctcggtg ttcgaggcgg tgaagcggat ggtcgaggcg aacgtcggag ccttgctcgt
     5461 gacggtcagc ggcgagatca cggggatcgt cactgagcgc gactacctcc gccgagcggc
     5521 cctggagggg cgcccggaca aggaaacccc ggtgcgggag atcatgtcgt cgccgctgct
     5581 cgtcgccacg ccggagacct cggtcgacga gtgcatggcg ctgatgaccg acaggcgcat
     5641 tcgccatgta cctgtggtcg acggcggcga ggtggtagga atcgtctcga tcggggacgt
     5701 cgtccggttc aagtcgaagc agcagagctt cgagctccag ttcctgcacg actacatcac
     5761 cgcaagctga cccgtccgcg agggctattg ggatcgtcgc cgaactgact cgcaactgct
     5821 ctgcgacgga gcggacgtac tggccccggg gagagcaatg aaggaggcac tccaaatgcg
     5881 gtcgtttacc aggttttcat cacgtagccg gcgcgggctc aggttcctcg ccgccgtctg
     5941 cctgctggcc ggcgcggcac tcgccctgtc gtccacggca tcggccgcgt cgatcgtgtc
     6001 cgccggtccg ctgacgtcga tcaccattag ccccgacctg aactgcaatg tgctccatac
     6061 gggcgacacc tttcccgagt ggtaccaggg cacggcgtgc ggcacgcttg ccgtcgacca
     6121 gtcgaccgcg acgctctacg gccccctgag cattcctgcc ggaggtgccg cggttccaag
     6181 gacggcgttc acgccgatca gccaggtggg cccgacgggg accggcacga gccttgaccc
     6241 gttcaagatc gtcacggtgg ccgccctcgg cctcagcggg ttgacgatca cccagacgga
     6301 ctcgtacgtc gtcggccagg agtcgtaccg caccgacgtg cagatctcga actcgaatct
     6361 cacctcgagg tccgtccggc tgtacgcggc cggggactgc tatttgcaga actcggatta
     6421 cggttacggg cgtgtcgacg gtagcgcgat cgcgtgcaca gcaacgtcgg atccaggcag
     6481 ccggatcgag cagctgttcc cgctcacctc cggcagcagc tacatggagg acaactacaa
     6541 cacagtgtgg gcgcaaatcg gtgcgcgggc tgcggggccg aacacgtgca tttgcgccac
     6601 gctcgacgac aacgggatcc tgctcagctg ggacgtgacc gtccccggcg caggctcggc
     6661 caccgtttcc cacctgacga cgttctcgcc gctcggtttc gcgccgctgt cgacgaccaa
     6721 gaccgccgac tcgtctactg cggccgcggg cggacccgac ggctacacga tcacgatcca
     6781 caacccgaac gcgtcgccgg tggtcgtgaa ctccgtcacg gacacgctcc cggctgggtt
     6841 cgcgtacgtg gcgggctcga caaccggggc gaccacgtcg gacccgacga tctcagctca
     6901 gaccctgacc tggagcggct cgttcagcgc acccgcgggc ggagacgtca ccttgcattt
     6961 caacgtcacg gcgtcgagca cgccggggac gtactacaac aatgcagctg gcgatgcggg
     7021 cgcggtggtg gtcgcggcga ccggcgacac cgctcccatc accgtggcag caggcgacga
     7081 gccaatcacc gcgaccggga caaccttctc tgcaaccgag ggggcgagct tcagtggcga
     7141 ggtcgccacc ttcaccgacc cggacaccgc ggcaacggcg agcgagtact cggccacgat
     7201 cgactggggc gacagctccg caacgacggc cgggacgatc ggcggctcgg cgggcagctt
     7261 tagcgtctcg ggtacgcaca cctacacgga agaagggtcg tattccgtaa ccgtgacgat
     7321 cgcggacgtc gacgacgcgg ccaacaccga cacggcgaac tcgacggcca tcgtcgcgga
     7381 cgctgcgctc cacgccggta cgttcacggt gccgccctcg acgtccctcg ggaacccgac
     7441 gaacgtctcg ttcagcttca gcgacgacaa cacaggggcg ccgacgagcg acttcacggc
     7501 cacgatcgac tggggcgacg cctccaactc gaccggcgtc atcggcggtt cgggcggcaa
     7561 ctacacggcc ggcggtagcc atacctacgc cgcagccggc ccgtacacga tcaccgtgac
     7621 ggtcgttgac gacggcggca gcacgaccgg cgccaccgcc gctacctcag tcgtcgtcgt
     7681 accgccggcg gctgctctct cggcgggcta ctggaagaat catcaggagc acacgacgtc
     7741 cctgctgtcg atcgcgctcg gcggctacac ggtcggcacg ttcgcggatg cgacggccgt
     7801 gttcgccaac atgaattgcg gcaagtcgtc ggcgaacgac gcggccggct gcctggccgc
     7861 acagctcctg gctgcgaagc tgaacctgaa gaacggtgca ggtacctgta tcgctccgac
     7921 gatcgcgagt gcggacgcgt tcctggtctc gatcggctac accgggccga acgccacgta
     7981 cactctgacg gcggcacagc gggcgcaggc gatcgagttg aaggacgtgc tcgacaagta
     8041 caacaacggg ctgggctgtt agttaacgag gtgaaggggg ccggcgcggg ccggcccctt
     8101 caccgctgcg cgaggcagta gggtagccgc gcgctttgaa cgaaaaacgc gtcatcggcg
     8161 tcgacatcgg cggcactaag atcctggccg gcgtcgtcga ccgggacggg cacgtggagc
     8221 accggcgcga gcatgcgacc ccgaccgcgt ccgaagacgc atttttcgcg ggcctggacg
     8281 aggcggtcga ggagctcctc acggacgaga tcgcggcgct cggtttcggc atcccgtctc
     8341 caattgacca gcgcacgggc cgggcgctgc aggccgtcaa cctcccgctg acggacatcg
     8401 ccttccgaga caggatgaag gagcgcttcg gcttgcctgt cgggatcgag aacgatgcga
     8461 acgcggcgac ctatgcggag tttcgtttcg gcgctgcgcg cgaggtcgac tcgatggtca
     8521 tgctcacgct cggcacgggc tgcggaggcg gggcggtcgt cgacgggcag ctcttccgcg
     8581 gctgggcgga gttcgggcac atcgtgatcg tgtacgacgg cctcccttgc cagggatcgt
     8641 gtacggggcg cgggcatctc gagccgtacg tgaccggggt cgcggcgacg aagcttgcgc
     8701 aggccgagtt cgggcccgcc gtcgatgcgc atcggctcgt tcgcctggcg aacgaaggag
     8761 aaccccgtgc ggtcgagatt ctcgacggca tcggtcgcca cctcggcgcc ggcatcggca
     8821 gcctcgtgaa catcttcaac ccggatctgg tcgtgatcgg cggcggtttc gcggctgccg
     8881 gcgacttcgt gctcgaccct gcgcgggaga tcctgcgccg tgaagcgctc gcgcgagccg
     8941 gctatcgagt gccgatcgag cgcgcagagc tcgggaccgc ggcgggcctg atcggcgccg
     9001 gcctcgtcgc gttcgacgta gccgggtaga accgtgccgc tcgccgtctg tgcgacgccg
     9061 atcggaaacc tcgaggacgt cacgctgcgc ctgctcgacg agctccgctc cgccgacgtc
     9121 atcctctgcg aggacacgcg acatacccgc gtgctgctcg accggcacgg gatctccggc
     9181 gcgaagcttc tctcctacca cgagcacaac gaggccaagc ggactgcgga gctgctcccg
     9241 cggcttctgg ccggcgaccg cgtcgctctc gtcagcgacg cggggatgcc agggatcagc
     9301 gacccgggcg cgcgcttggt cggagccgct ctcgagcagg gtgtgccggt gactgtgctc
     9361 ccaggcccgt ccgctgtcga cacggcgctc gtcgccgggg ggtttgcggc cgaccgctac
     9421 cagttcgtcg ggttcctgcc gcggggcgcc ggcgcattga cggctctgtg gcgcgagctt
     9481 gcaggctggg cacatccggt cgtcgcgttc gagtcgcccc agcggctgcc tgggacgctt
     9541 cgttcgttgg cggctgtgca gcctgggcgg gaggtggccg tctgccgcga gctgacgaag
     9601 aagttcgagg aggtcgtgcg tggatcggcc gcggaagtgt gcgcccgctt cgtggagccg
     9661 ccgaagggtg agatcacgct cgtgctcggg ccgggcgaag acgaacggga tctgcccgcc
     9721 gcactcgagg cggtcgccga actggtcgcc gccggagttc ctcgccggca ggctgccgag
     9781 gtcgtttcgc ggctgaccgg cgcgtcccgc aacgagctct acagccgtac tttgtagcca
     9841 gtattgacaa caaagaccgg cctcgctacg gtcgcatcgt tctcaaaact ggcaacagga
     9901 ggaactgtgc ggcgacttgc ggtcctgatc atttgcgcgt gtgcgctccc tgtcaccgac
     9961 gctcacgcct ggacgtggcc cgtggacggt ccggtgctgc ggccgtttgc gttcgaccac
    10021 gcgcatccct acgcggccgg ccagcatcgc ggcgtcgatc tcggcgcccc gagcggaagc
    10081 cctgtgctcg ccccggcgaa gggaatcgtc tcgttcgcgg ggacggtgcc gaccggcggc
    10141 aagactgtgt cgatcgagac ggcgctcggc tacaccgcca cgctcgtcca cctcggatcg
    10201 atcgccgtgg tgcgcggggc gctcgtgggc gaaggctcgg tcgtcgggac ggttgggccg
    10261 agcggcgtgg tcgacttgcc ggaaccctac gtctacttcg gtacgcgcat gacgagcgat
    10321 ccgcagggat acgtcgatcc gctgaccatg ctgccttcgc gcgaggcgcc tgcgccctcg
    10381 ccggctgccg agcctgcagt cgtcgccgtt cctctggaga cccgcgaagc ggcgtcggcg
    10441 gcgacggctg gcccgtcgcc ggcaagcgag ccaagcggca actcggaacg aagcacggcc
    10501 gagagcccgg cagtgtctcc acaggaggag gcggcgatcc agccgacgat tgcgagcggc
    10561 caagcgacga cgcttcggcc cgccagctcg cgagttgctc ccgacgtggc tgcgcccgag
    10621 tccaagacgc cggcgaaacc ggcagaggtg cgtccccgct cgcgcgcgat tgtcgcagcg
    10681 gcgacgccgc tggctaaggt gaagttgggt cgcgctcggg gctcggcccg ccccggcttc
    10741 gatgggcacg cggttcgcgc gcaacggtcg gagcggctga cgacggttga ctcgcgaggg
    10801 gcggggatcg ctcggacgtc gcgcggatcg gccggcggca gctggcgact ccccgcaggt
    10861 ctcgcggctg ccgcgaccgc ggctctgctc gcattgcgtc gtcgtctgcg ccggaagccg
    10921 gtcgacacgc tccctatcat ggattcaggt gaacagcttc tacatcacga caccgatcta
    10981 ctacgtcaac tcgacgcctc atatcggcca cgcgtacacg acgatcgtgg ccgacatcat
    11041 cgcgcgccat cacaagcaac gcggcgacga gacgttcttc ttgacgggca cggacgagca
    11101 cggctccaag gtctaccggg cggccgagga gcaggggctc gacccgaaga cctacgtcga
    11161 ccagatcgcc gatgcctgga aagagctgcc taggcgggtc gatgccgact acgacttctt
    11221 cattcgcaca accgacgagg gacacaagcg gttcgtccag gagttcatcc agcggatcta
    11281 cgacaacggc tacatctacc aggacgtcta ctccggtctg tactgcgtcg gctgtgaaga
    11341 gttcaaggcg gaggccgagc tggtcgatgg caagtgtccg attcacggca ccgtgcccga
    11401 gcgaatcgag gagacgaact acttcttcaa gctctccgct tttcaacagc ctttgctcga
    11461 cctgtatgac gcgcggccgg acttcgtcct gccgcgcttc cgctacaacg aggcgcgctc
    11521 gttcatcgag ggcgggctac aggacttcag cctcagccgc gcgggccagc cctggggcgt
    11581 cccggtcccc tgggacgaga gccaggtcgt ctacgtgtgg gtcgatgcgc tcatcaatta
    11641 tgtgagcgca ctgacctacg cacgagaggg cgaggacctc cgcgagaagt tctggcccga
    11701 ggtgcgccac ctgctcgcca aggacatcct gcgcttccat tgcgtcttct ggccggctct
    11761 gctcttggca gcgggctacg aggttccgaa gcagctcttc gtacacggct ggctgctggt
    11821 cgacgagagg aagatctcga agtccgtcgg gaacgtcatc gacccgctcg atctcgtcga
    11881 cgtctacggc agtgacgcgg tccggttctg ggcggtccgt gccgtctcgt tcgggcagga
    11941 cggaaacgtg acgctcgaaa gcctgcatga gcgatacgag agcgagctcg cgaacgatct
    12001 gggcaacctg gtctcgcgca cgacggcgat gatcgcccgt taccgggagg gacggatccc
    12061 ggcggccacc ggagagagca aggtgtccga cgcgatcgaa gcggtgaaac gcgaggtgcc
    12121 ggctcggctc gacctgttcg acgtcaccgg cgcagtcgac gtgatctggg atctggtccg
    12181 ctccctcaac aagcacgtgg agcagacgaa gccctgggag ctggcaaagg acgacgcgca
    12241 cgcgggcgag ctcgatcgtg tgctgtacga cctggccgac gggctgcgtg tcgccgcgat
    12301 cgcgctttcc gcctatctgc cgcagaccgc gccacgcatc ttgcaggcgc tcggccagcc
    12361 cgaggacctc gcctggaaga acgtcgaggc tggccgcacg gtcaccgcag ctgggatcga
    12421 gcccgcgacg ccgctcttcc cgcgcgtgga cgctcccacc gccgctgcgt gatcgatacg
    12481 cacgcgcatc tcgaagcgct cgacgagccg tcagccgcaa tcgcccgcgc ccgcgatgcc
    12541 ggggtggcca ggatcgtcac gatcggaacc gacccgatct cgtgggccaa gatgctcccg
    12601 atcgtcgacg agcacgacgg cgtctacggc gtcgcgggca tccatcccca tgcggcggcg
    12661 tcggagttcg acttcagcgc gctcgagcgc ctgctcgccc atccaaaggt cgtcgcggta
    12721 ggggagatcg ggctcgacta ctaccgcgac tacgcaccac gcgatgctca gaccaaggtg
    12781 ttcatctacc agctcgggct tgcgaagcga gcgcagctgc cggtcgtgat ccacaaccgc
    12841 gccgctgacg acgacacggt ctccatcctg cggcaatggt tcgacggcac ggtcgtgctg
    12901 cactgtttct cgtcggccgc gttgctccct gaggccgtcg agcgcggctg gtacgtctcg
    12961 ttcgccggga acgtcacgta cgcgaacgcg ggggacctgc gcgacgccgc gcgtgccgtt
    13021 cccgccgagc tgattctcgc cgagacggac tgtccgtatc tggcgccgca gcctgttcgg
    13081 ggcaagacga acgagcccgc atacgtggtc cacacggtgg ccgcgctcgc ggaggctcgc
    13141 ggcgaggagt cggatgctct cgccgcgcag atcgacgcga acgcgaccgc ggccttcgga
    13201 ctgccgtgag gcgacggagt cgccggtccg cgaagcggac caggggaggg ggttgcgggg
    13261 gaaccgggag gttcgcccgg gtacgacgat gagcatcagc cccaaaaggc gcctcgggca
    13321 acacttcctc gtcgacgaga acatcctcgg cgtgatcggt cgcctggcgc agctggacga
    13381 agacgacgtc gtgctggaga tcgggcccgg cctcggcatc ctgacgacgt atctcgcgga
    13441 tcgcgtttcc caggtctatg ccgtcgagct ggatgcgtcg ctgggaccgc agctcatcga
    13501 gcggctggcc ggccggccga acgtcgagtt gcgtttcggc gatgccgtga agctcgacct
    13561 tgccgaggtg gcgccggggg cgaagaagct cgtcgccaac cttccctaca acgtcgcgac
    13621 gccgttggtc gccgagagcc tcgaccacct gccggggatc gagctctgga ccgtgatggt
    13681 gcagcgcgag gttgccgacc gcttcttcgc cgtgccctcg accaaggctt atggcgcggt
    13741 gtccgtcctc gtgcaactcg tggcggagcg gacgggcttc catcccgtgt cagcaacggt
    13801 gttccggccc cggccgaacg tcgactctgc gctcgtcgcc ttccggcgca cggggctccc
    13861 tcacgacttt cgtcgcgtca agcgagtggt cgagggctcg tttgcgcaca ggcgcaagcg
    13921 cctggcgaac tcactggagc tcgcgggagt cgcggaacgc ggtcgcgtcg aggccgcgat
    13981 cgccacgatc ggtagagaac cgaacgttcg cgccgaagag ctcgcgcctc acgagttcct
    14041 cgcgctcacc gaggccttga agtgacgcgc gcacccgcgg ccgccaagat caacctgtca
    14101 ctcgtcgtcg gcccggtgcg cgacgacggg aagcacgagg ttgtgacggt ctaccagcgc
    14161 gttgcgctct ccgaccgaat cgccgtcgag cgttcctccg agctgggcgt cgccgggttt
    14221 gcgggcgaca cgctcgtccg ccgtgcgctc gaatgcatgg tcgacggcga cggcgctagc
    14281 ttcactgcgc ggatccagaa acgcattcct gtggccgcgg gactgggcgg cggcagctcc
    14341 gacgcggcga ccgcgttgcg gttggcaaac ggactgcgaa ccgatcctta tcccgcggaa
    14401 cgcctacgca tcttcgcgcg cacgttgggg acagacgttc cgtacttcct ggaggacggg
    14461 ccgcagctcg gcacgggcga cggcaccgac ctcgaagcac tcgagcttcc ccaggattac
    14521 tgggtgctgc tggtcctgcc gcgcaaggcg ttgaagagct cgaccgagca cgtgtacgcc
    14581 gagttcgatg cacgcggagg cgagcacggc tacggcgaac ggcgcgccgc gctgctcggc
    14641 gcgctggagc gcgtcaagcg tccgcgtgat ctcgcaacgc tgccttcgaa cgacctcgca
    14701 tcgtccccgc tggcgcgcga actcgttggc ctcggcgcct ttcgtgcaga cgtcacgggt
    14761 gccggtccga ctgtctacgg gctcttcttg cacggccacc aggcgcgagc agcgcagaga
    14821 caaatctccc ccaaagggcg cacgtggctc acggcacctg cgtggtacgg ttgaaaacgt
    14881 ggcgtacagc cagccgatga tcgaaggcgg cagcacgcgc atcggccggt ggctccgcga
    14941 acggcgactg cgcctcgctc tctgggttgc cgtggtcgaa gggctgctcg ttgcgctcac
    15001 gcccgacctg acgaagtgga ccgtcctcgt catcggcgca atcttgctcg ctttctacat
    15061 tgtcgcgggt cgtagcatgc ggtgggacgt cgcgcgtcag ctgtcgtgga tcgccgcggc
    15121 gtcgcaggcg ctcgcgatcc tcgtggtcat cctcgccttc gttctcaagc tcgtggcgat
    15181 cgttgcggtc gtagtcttcg cgctcatcgc gcttgcgtat ctcttctcgg actctcggcg
    15241 cacctgagcc tcgttttcag gatgtcgaca cccatgccgc ctcaggcaag aggggcggtt
    15301 ttccgcccct ctcgagccgc gctcaaaccc gtgtgctcct atttagctcg gaagcaagac
    15361 caggtgtcgt tacgcgggat gtagatgtac tgcccgttgg gctgctgcgg acacggtggc
    15421 gtgccgcgga ggtagatgtc gggccgcagt aggtgctgga cgccgatgaa ctcgccggtc
    15481 gcgaacgcga agaccaggag ttcgtacgag ggtcctcgat cggcaaagcc gggtgtgccc
    15541 ggaagcgggg gcaggccgag accgggcggc gcgcagattc gccgggtcgg atctgccaga
    15601 ggctggacac aggtccagcc ggcctgcgtc agttgatccg gcgttacccc tgcactactc
    15661 ggggccgccg tgatggctag tacagctgcg agagtcgcgg ccacgacaag cagcgaacga
    15721 atcgcgtgct tcatcggaga cctcctccat ctccctgcat acagaaacgc cgcagcgtct
    15781 ctccttcgat cgattctccc actgggaacc cggcctgaca acctgacggg caggtgcaga
    15841 tcattctgaa acgagttcta cgtatcgttc gagtaccaga tacccaggtg gggcgtagcc
    15901 aagtggtaag gcagcgggtt ttggtcccgc gatcccaggt tcgaatcctg gcgccccagc
    15961 tctgatgcgt agcgaactcg cagccgtcgt catggcggcc ggactcggca cgcggatgaa
    16021 gtccgcgacg ccgaagcacc tgcacccgct gctcgggcgg cggctcgtcg actgggtcgt
    16081 cgaggccgtg cgcccgctcg gcgcggatcc gctcgtcgtc gttctctcgc cggaagcagc
    16141 gaacgcattg gacggcgtga ccgttgcggt ccagcagcgg ccgctcggca ccggcgactc
    16201 gctgcgcgcc gcgcgcgaga gcgtgggcga cgccgagacg gtgctcgtcg tctcgggtga
    16261 ccatccgcgc atcagctcgg acctgctcca gaagctcgtg acagagcatc gctctgccgg
    16321 tgctagcgcg accgtgctct cgttcgagcc gcccgatgcg ctcgcgtacg gccgcatcgt
    16381 ccgctctgcc gacggtcgcc tgaaggcgat cgtggaggat gcggatgcga acgacgccga
    16441 gcgtgcgatc agagaggtca attcgtcgat ctatgtcttc gcggccgaca agctctggcc
    16501 cgtgctcgac cgcatcgagg tggcgaacgc gcagggcgag ctctacctca cggatgcggt
    16561 gcggctgctg gtcgaaagag gagaggaggt tgtcgtgttc aaggctccgg atgccgatga
    16621 agtggaagga gtgaacacac gtgtggagct ggccgacgca gctgctttcc tacgcgcgcg
    16681 cgtgaacaga gaacacatgc tggcaggcgt caccatcgt
//
LOCUS       DASVEU010000198         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_528251,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000198 DASVEU010000000
VERSION     DASVEU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528251"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF225_08220"
     CDS             <1..243
                     /locus_tag="VF225_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7145280.1"
                     /translation="VNAVHDPVLDKAREVGALLASKSPIALRLMKELANRALGGDHAQ
                     NLEAEGETFGELFSSEDAREGLAAFVEKRAPVFRGR"
     gene            complement(240..1124)
                     /locus_tag="VF225_08225"
     CDS             complement(240..1124)
                     /locus_tag="VF225_08225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7145281.1"
                     /translation="MRRHRTTARGVLFLAATIGLAVGSAVAFAATVPEHIIFPVVGKV
                     AYIDDFGQPRPGGPHQGNDIIAEKKSPAVAAEAGKVKYWTTSSAAGCMLYLYGESGTT
                     YLYIHLNNDLTLKNDNRGKCVKGTAYTVKNGAKVAAGQQIAYVGDSGDADGRSSHLHF
                     EVHPGGGKAVSPYPYLQKAYRLLFTAKPGTAFALTLTGTVVSAAIDRLVVNVSTSQAW
                     PSGLTLTKLNRTIALTVPESTVVQSLSSTGTFRAVANVTLAQKGDKVVVWTQPASATL
                     KAQRADDGVLNSALIQLG"
     gene            1213..1986
                     /locus_tag="VF225_08230"
     CDS             1213..1986
                     /locus_tag="VF225_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7145282.1"
                     /translation="MKIAVCVKQIPEGHSRIDPETKRLDRSGEGALNGFDANAVEEAL
                     RLKDATGAGEVVLVSLGPAKAQDALRKALAMGADRSVLVTDDAAAGSDLVATSYTLAK
                     ALEREQADLILFGQQAGDSDGAVLWAAVADRLRLPVVSQAAEVTHADGKLTVKRQTEF
                     GYDVIEAPLPAVVAVSDAINEPRYPSLKGIMGAKSKPQEIVSLSDLGVESDRAGEQGS
                     RTEVYALNDPPPRGDSKKIEDDGNGAQAILDFLVEKRHV"
     gene            1983..2945
                     /locus_tag="VF225_08235"
     CDS             1983..2945
                     /locus_tag="VF225_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7145283.1"
                     /translation="MTLVFLEHHGDELLKGSLGVLSKAATLGDAEVAGVVIGSGVRAL
                     ADQAGKFGAAKVYVADDAQLERPLPQPRVDVLAQLVRDKGIDTVLFGNSVLGADVAAG
                     LAARLDAGLNWDLADLVQQDGQLVGKRPALQDSVYVDVGWTSEPRIALFRSGSFDPTE
                     VGGSADVEDVTVALEDFSTSARMVDQAHEESEGPSIEDAPVIVAGGRGLGGPENFTLV
                     EELAKALGGAVGATRAVVDAGWYPYSTQVGQTGKTVSPKLYFACGISGAIQHKVGMQS
                     SGVIVAINKDSNAPIFEFSDLGVVGDVHEVVPKLTELVKARKAS"
     gene            2942..>3421
                     /locus_tag="VF225_08240"
     CDS             2942..>3421
                     /locus_tag="VF225_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145284.1"
                     /translation="MTKPADFPPLFRAADYIAAPSDSPEERIEVGVLIVGAGPAGLAC
                     AIRLGQLLEDAPELRERLGEVPVAILEKGKQPGSHLLSGAVVNPRALRRLFKGRKRID
                     EMPFYGPVDHESVYFLTKQNALRIPAPPTMKNEGNYVASLSRLGRWLAEQAEEAGVML
                     "
BASE COUNT          562 a         1069 c         1199 g          591 t
ORIGIN      
        1 gtgaacgcgg tgcacgatcc ggttctcgac aaggcgcgcg aggtcggcgc gctgctcgcc
       61 tcgaagagcc cgatcgcact cagactgatg aaggagctgg ccaaccgtgc gctcggcggc
      121 gaccacgcgc agaatctcga agccgaaggt gagacgttcg gcgagctgtt ctcgagcgag
      181 gacgcgaggg agggccttgc cgctttcgta gagaagcgcg cgccagtctt ccggggtcgc
      241 tagccgagct ggatcagcgc tgagttgagg acgccgtcgt ccgcacgttg cgccttgagt
      301 gtcgcggacg ctggctgcgt ccagaccacg accttgtcgc ccttctgggc gagcgtgacg
      361 ttcgcgaccg ctcggaacgt gccggtggag ctcaggctct gcacgacggt cgactcgggg
      421 acggtcaggg caatcgtccg gttcagcttc gtcagcgtca gtcccgaagg ccacgcttgc
      481 gacgtcgaga cattcacgac gaggcggtcg atcgcggccg aaacgacggt tcccgtgagc
      541 gtcagtgcaa acgctgttcc cggcttggcc gtgaagagga gcctgtaggc cttctgcagg
      601 tacggatacg ggctcacggc ttttcctccg cccggatgaa cctcgaagtg caggtgcgaa
      661 ctgcggccgt ccgcatcacc cgagtcgccg acatacgcga tctgttggcc ggccgccacc
      721 ttggccccgt tcttgaccgt gtacgcggtt cccttgacgc acttgccgcg gttgtcgttc
      781 ttcagcgtga ggtcgttgtt caggtggatg tataggtatg tcgtgccgct ctcgccgtag
      841 aggtagagca tgcagccggc cgcgctcgac gtcgtccagt acttcacttt gcctgcttcc
      901 gcggcgactg cgggcgactt cttctcggcg atgatgtcgt tcccttgatg cggtccgccg
      961 gggcggggct ggccgaagtc gtcgatgtag gccaccttgc cgacgaccgg aaagatgatg
     1021 tgctccggaa ctgtggcggc gaaagccacg gctgagccga cggcaaggcc gatcgtcgcg
     1081 gcgaggaaga gaacccctcg ggctgtcgtc cggtggcgtc tcatgagagg acgggcgctc
     1141 cgggtcgcgc tcgaagggtg ggacggtagc aggcgccttg ctacaagtca gcctcagtag
     1201 agtcctgcac cgatgaagat cgccgtctgc gtcaaacaga tccccgaagg gcatagccgg
     1261 atcgaccccg aaaccaagcg cctcgaccgc tcgggggaag gtgctctgaa cggcttcgac
     1321 gcgaacgctg tcgaagaggc gctgcggttg aaggatgcga cgggcgcggg cgaagtcgtt
     1381 ctggtctcgc tggggccggc caaggcccag gacgccctgc ggaaggcgct ggcgatgggc
     1441 gctgaccgct ccgtcctcgt caccgacgac gcggctgcag gatcagatct cgtcgcaacg
     1501 agctacacgc tggccaaggc gctcgagcgc gaacaggcgg acctgatcct gttcgggcag
     1561 caggccggcg actcggatgg tgcggtgttg tgggcggcgg ttgccgaccg tctgcgtctg
     1621 cccgtcgtgt cgcaggcggc ggaggtcacg cacgcggacg gaaagctgac ggtcaagcgg
     1681 cagaccgagt tcggttacga cgtgatcgag gcaccgctgc ccgcagttgt cgccgtttcg
     1741 gatgcgatca acgagccgcg gtatccgtcg ttgaagggga tcatgggagc gaagtccaag
     1801 cctcaggaga tcgtgtcgct ctctgatctc ggcgtcgaga gcgatcgcgc aggcgagcaa
     1861 ggctcgcgca ccgaggtcta tgcactgaac gacccgccgc cgcgcggcga ctcgaagaag
     1921 atcgaggacg acggcaacgg cgcccaggcc atcctcgact ttctcgtgga aaagcggcac
     1981 gtatgacgct cgtcttcctc gagcatcatg gcgacgagct gctgaagggg tcgctcggcg
     2041 tgctctcgaa ggcggcgacg ctcggtgatg ccgaggtcgc cggcgtcgtg atcggatcgg
     2101 gcgtgcgcgc gctcgccgac caagcgggaa agttcggggc tgccaaggtc tatgtcgccg
     2161 acgacgcgca gctcgagagg ccgctgcctc agcctcgcgt cgacgtgctc gcgcagctcg
     2221 tgcgcgacaa agggatcgac acggtgctgt tcggcaactc cgtgctcggc gcagacgttg
     2281 ctgctggact ggccgcccgg ctcgacgcag gcctgaactg ggacctggcc gacctcgttc
     2341 agcaggacgg ccagcttgtc ggtaaacgac ccgcgctcca ggattccgtg tacgtcgacg
     2401 tcgggtggac gtccgagccg aggatcgcgc tctttcgctc cggatccttc gatcccaccg
     2461 aggtcggtgg ctccgcggac gtggaggacg tgacagtcgc gctcgaggac ttctcgacgt
     2521 cggcgcggat ggtcgaccag gcgcacgagg agagcgaagg cccgtcgatc gaagacgcac
     2581 ccgtcatcgt cgccggcggg cgcggcctcg gcggtccgga gaacttcacg ctcgtcgagg
     2641 agctcgcgaa ggcgctcggc ggcgctgtcg gcgcgacgcg cgccgtcgtg gatgccggct
     2701 ggtacccgta ctcgacgcaa gtcggccaga cgggcaagac ggtgtcgccg aagctctact
     2761 ttgcgtgcgg catctccggc gccatccagc acaaggtcgg catgcagagc tccggtgtca
     2821 tcgtcgcgat caacaaggat tcgaatgcgc cgatcttcga gttctcggat ctcggcgtcg
     2881 tcggtgacgt gcacgaggtc gtgccgaagc tgaccgagct cgtcaaagcg cggaaagcgt
     2941 cgtgacaaag cctgcagatt tcccacctct gtttcgtgcg gcggactaca tcgcggcgcc
     3001 gagcgattcg ccggaagagc gcatcgaagt aggcgtcctg atcgtcggcg ccgggccggc
     3061 cgggctcgcg tgtgcgattc ggctcggtca gctgctggag gatgctccgg agctccgcga
     3121 gcggctcggc gaggttcccg tggccatctt ggagaagggc aagcagcccg gctcgcatct
     3181 cctctcgggc gcggtcgtca atccgcgtgc gctgcgacgc ctcttcaagg gacgcaagcg
     3241 catcgacgag atgcctttct acggccccgt cgaccacgag tccgtctact ttttgacgaa
     3301 gcagaacgcg ctgcgcattc ctgccccgcc gacgatgaag aacgagggca actatgtggc
     3361 gtcactgtcg cggctcggcc gttggctcgc ggagcaggcg gaggaagcag gcgtgatgct
     3421 c
//
LOCUS       DASVEU010000199        11066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_913881,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000199 DASVEU010000000
VERSION     DASVEU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913881"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF225_08245"
     CDS             complement(<1..214)
                     /locus_tag="VF225_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145285.1"
                     /translation="MSRDAADQILPHGARLDVGKDVPQFVNEPVLGFGRADVSPLEMM
                     LHTEAGESPSFGYELAYRRRAFALQQV"
     gene            complement(968..1276)
                     /locus_tag="VF225_08250"
     CDS             complement(968..1276)
                     /locus_tag="VF225_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145286.1"
                     /translation="MRDLSAIDRTIRGTPIATLAELQTYAYEVPEDKAPPALFSRLTA
                     GEEVEPGELFTSLDTEGRVAAGDSDAALEIIETIKRRLRAAFPDEVPDTDVWGLDSRR
                     "
     gene            complement(1390..1875)
                     /locus_tag="VF225_08255"
     CDS             complement(1390..1875)
                     /locus_tag="VF225_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145287.1"
                     /translation="MLPSLSVDHPSQDAFGGLEALYLELHRRQVAASPSRMGLAATTD
                     QVAWERRRAFYIESGSFVVRAHANHRLIGYAAAGMRPGFAGWGPEAPMGIIHDLVVTE
                     PARRQGVGTLLLHRVRSELKATGAVALQLSVVRHNADALAFYSHNGFETIAETLGVAL
                     R"
     gene            2045..3358
                     /locus_tag="VF225_08260"
     CDS             2045..3358
                     /locus_tag="VF225_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145288.1"
                     /translation="MSPDGRVTLRYALDDVEEFTETFDIPLEPGVIADEDAVRPLLDL
                     LHWVAGVSYYKLAAPPSVSCESGAPPPAAAAYLEALYSEGLGEFAYANRDRLPRLPTP
                     EFAPGPALRPTGGAVRQARSPRRVLVPVGGGKDSVVALEVVKRSGVGCALFVVNCLDP
                     MRRTAEVAGLPMLEVARSLPLATFKRLHAAGALNGHVPITAIISCVALLTARLNGYDT
                     VALANERSASVGNLIHDGVEVNHQFSKSARAEGLLRAAAAEASTDVDIYSLLRPASEL
                     VIARAFATKLGAYHHAFTSCNRNFQTDPALRGGEAWCRDCPKCRFVYLMLAPFMAPEP
                     LAEVFGGAMLDDERQYEGFALLTATGGHKPFECVGEEEESLAAIELLDARDAWRDQVV
                     VRRLVAEALEVHGRDSTRIGSFLRLAEEHAIPAQLADELRAFLGP"
     gene            3339..4676
                     /gene="murD"
                     /locus_tag="VF225_08265"
     CDS             3339..4676
                     /gene="murD"
                     /locus_tag="VF225_08265"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7145289.1"
                     /translation="MRFSDLDGCSIGVWGAGVETRSFARVLRSSLTRARIAVVVLEDA
                     PDAPELTADARVVSASEAFEALRTCDVLVRSPGVSRHRSELRAIAAQGTPIVTPTGLW
                     LAERGGRNTIGITATKGKSTTASLVAHLVAATGRGVQLAGNIGRPALELLDYPDTELA
                     VLELSSYQIADLTCGPQVAMAGNVYREHLDWHLTDDAYRQDKLRLLALPGVERCVVNA
                     LSPEVMAAERAPGPVYVYGTAPGWRVDDDGSVMKDGAPSLARDVLPLRGRHNALNLCG
                     ALTALEALDIPLPPLPAAFAGLQPLEHRLQTVHEGDGVEWVDDSISTTPESTLAAMAA
                     FPDARLIVIGGGFDRGQDYAELGLVAARSRALVVTLPVTGSRLAQAVRAAGGEAYEVA
                     DLPAAVAAARALATPGTVVLLSPAAPSFNTHVDYRARGDHFRELAATTGGPNP"
     gene            4673..5629
                     /locus_tag="VF225_08270"
     CDS             4673..5629
                     /locus_tag="VF225_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145290.1"
                     /translation="MTPPEPAVLSPAGDPLTWVDAWARSPDLARVVAAFGGETSGQPD
                     LGAWLAYLEAFSQRWDYRAGQERNLVKAPDFTPDLTAMVFAAATVLGMRGTTAPAREH
                     YEHVVILGGLVRACLARPRHAANLLDAGTITADRIIALGGFRPLKGDEETLVAELLDT
                     SVTDEYHAMRAGVEAAFGLGAPVSTRGEPSEVVGASWGVTDYVTAAGVAAHVVAAPSS
                     EPGVRRANTPDTYAWLARDSTWLRRGDAVLLVTTDIYAPYQGADAIRMLAVPHGVEVD
                     VVGIEPGGIDPRLAQPFAVHNYLQELRSTIRSLRMLHSVLTG"
     gene            5723..8179
                     /locus_tag="VF225_08275"
     CDS             5723..8179
                     /locus_tag="VF225_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7145291.1"
                     /translation="MSVNRDWPGLVLRLAQARGGDGQLCPQTFVLGGSHETRVTVVAQ
                     QARAINLAWALHRTGRLVGRDGKPSSVGVVGAGAAGATFAAAAGLLGANVTLVDEHEE
                     PITTQRWSFDRYLHPNLFDWPVEGWNQERAGLPLMDWAAGPSARVRLELLAGFALAAA
                     RGGVTWCSQHSVSEVDSADGKVKLTVCDLRSTAEPDAAGSVRVAEESYDLVVVATGFL
                     PEPRVGDRTTMSGSYWKDNDLAQVLDDSEQRHHTIVGDGDGALTELLRLSLIGREAKR
                     EFNQSMLTTLATQLLDADRLREDVLSVEAGISGTRGKGTLHAYASGSFDGADDSLRDW
                     LRPSAGGVEVRAGRVTSLRNAFALNRFLAARLTAGSSVRFLRGAPFLEADVPALAPSR
                     VVWRTGPRPRVRTLLVRPALAVKDAGDALAGDPSRQDVLGTVDDLMDASREPRWDARA
                     RELLAVPAEESALKAAVDLERGGLAVSRGFGWKPAGWPASVDAPPLPELPDMILDLAR
                     MALSIGNGPPPEVWSNFLVNHDSVAVSLDLVAVAADRTPTEIIDQLRRTDAPGIRLVL
                     IHVPRGPSDLERVWIRVDPAQAAAHLSKPGWTAVSLLLAPGFAAGDSFDDGETRRFES
                     HAAPRTEDSALDVLAERLLNERAIASTQWVTADRITAAVAERSSEQTLLQAAREGDAR
                     LERALVTVLHRRLGDGDPYEFRSALLGLAALVGTDDDRPGSVTDVYLATVLALPAGDR
                     AGALVRAALGPLVRLDPATGELVPLSRYDDRLRSESPEVLAAHDGPPHDGAPHGRGVP
                     DFSDFAVRVSSTDTERAEDA"
     gene            8172..>11066
                     /locus_tag="VF225_08280"
     CDS             8172..>11066
                     /locus_tag="VF225_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145292.1"
                     /translation="MPDPPTTSWPLWQRGRAAKTEDAEYVALARYATTDALWGFDAPE
                     LPAGLSAPEIVARVYGEVLAGAGWNYVEEPKRPAPLSSLASQQIRTPRDIQRYRAATC
                     LDASLLLTALAVRAGTRPHLAVGRNHAFVIVGDHVGGTATGTGGIAYASRATADLVGD
                     GVAMAIDVVQALVTPKRAQAASFADALAEGQRWVADAVTSGQRLMLIDVVAANERHGF
                     TPYAPDRDPTFIPRSLPAHAGALKLYESQESICERLRGKHGVIVIHGDSGSGKTTAAL
                     HLAAERESGPAVILDASSGETIVTSAAEVGARERGVSVGRLEPQERDGDAASLLARLS
                     TSPGSWCVVFDNADGDPSQVLRWLPRPDREKGQAVIVTTTNPTWLQLSLVNEREALAP
                     IDDTDVARALGTHSALAELVAGRAVTLRAFLRYLSDGGGDAARLQRHAEALPDDELRG
                     PVALVSAVLEEYGELGPVLQRLELLPPDHLPICDDVADYATFQRLEALGLVDLTPEAG
                     VARMHRLVGRAAERVLGQDDEARTAADMVRGAETFAALSAAAEPSLLERYTKLILAAF
                     AARQPTFLIDGFVLQRAASLMELRGRVEESRQLAEPAVTVLGAVAGAEPIELADALQS
                     LARWTNQHDPRNAVALAAALEQMALAQSVAARFGDERRAARSRRLEALLRLKVGWLNA
                     DLETGTRDIVAALGDLEREADAAERPDSGVDRGEALRARFNVAGPRVTLAQRVPSEAD
                     GHLREAQRVYEVVGHRRQALYPQTQHPHVAACISGEALARYMRANLVAVTYDERQRLL
                     AEATDLEREALTDRFELEGPLGGADTSKSSKLLTKISYARHRLNDSAVSSTVLDEARG
                     EIAVPRLTAATADHRAYAERWMSAPQLAAVLQVFGVSTPAALEPVAAERTIYDVLETR
                     TILSAARALGLMDVALPVRRVYDQIVVLDGDGRFAERAAGVVERLVSGGEV"
BASE COUNT         1657 a         3786 c         3867 g         1756 t
ORIGIN      
        1 cgacctgctg caacgcgaag gcgcgccggc ggtacgcgag ctcgtagccg aagctggggc
       61 tttcgcccgc ttccgtgtgc agcatcatct cgagcgggct gacatcggca cggccgaagc
      121 caaggaccgg ctcgttcacg aactgcggga cgtctttgcc gacgtccagc cgggcgccgt
      181 gcgggaggat ctgatcagct gcgtcgcgcg acatctggag ctaaagccgt cgctgctgcg
      241 ttcgttgttg tcgagtccgg agagccgggc cgagacacgt ccgacggggg tgtccgccgc
      301 cgatcccacg caagcgacca cggcgcatga agggagccgt ggccttcttc ttcagtgcgt
      361 cgctgatcct gagttcgccg cgcggctccc gtcaggagac gcgctggtgc ggctgttccc
      421 caatgagttg cagcgacgaa ctgccgagca cctccgcgtt catgcgcatg accctgccgc
      481 ggggcttccg gacgacgacg aagtcgtggc gctcatcacc agccttctca cggcgcccat
      541 ccccgtctag ctgtgtcccg ctgcgagcgc gcacggcggg gcgcagccca cggggctgag
      601 gcacgaacag acccaggcag gtggcgtgcg gcgggacact cccaggggtg tagagcatat
      661 ggcgggcatc gccaagagac agcagggtcc cctctcagcg gcgtgtgggc accaagtcgg
      721 aaggccggat gtcttcacga ggcgacaagc agatccgctc ggctgtagac cctcgcttcg
      781 tcgcggccgc gaggaagccg cgagactcgg cgtcatcgaa gggtgaggac ttgtgcagca
      841 cgacgcgcgc tggctgagtg gtgctactgg cagagtctcg acggtcagat cagcgatcta
      901 ggcgaccact cgcgctgctt cgcgcgtcgt gtggccgcga tcttgttcac gagcaactcc
      961 ccgttgttta gcggcgggag tcaaggcccc acacgtctgt gtccggaacc tcgtctggga
     1021 acgcggctcg caggcggcgc ttgatcgtct cgatgatctc gagagcggcg tcactgtccc
     1081 ccgcggcgac gcgaccctcg gtgtccaacg aggtaaagag ctctccgggt tcgacttcct
     1141 cgccagcggt caagcggctg aagagcgccg gcggggcctt gtcctccggg acctcgtacg
     1201 cgtacgtctg aagctccgcc agcgtggcga tcggcgttcc tcggatggtt cggtcgatcg
     1261 cggacaggtc gcgcatgatc gatctatcgt accgctggag cgtgcttggc cggggagagg
     1321 tttccttcca ggtagccagg ccgcgaattc tcacgctgaa cgccgttgag cgcggacctg
     1381 cacggatacc tagcggagcg caactccgag cgtctcggcg atggtttcga atccattgtg
     1441 cgaatagaag gcgagagcgt cggcgttgtg gcgaacgacg cttagctgaa gtgccactgc
     1501 gccagtggct ttgagttccg atcgaacgcg gtggaggagc aacgtcccga ccccttgccg
     1561 acgtgctggc tcggtgacga ccaagtcgtg gatgatgccc atcggcgcct cgggccccca
     1621 tcccgcgaat cctgggcgca tccccgcggc ggcatagcca atgagtcgat ggttcgcgtg
     1681 ggcgcggacg acgaacgatc cggactcgat gtagaaggct cggcgccgct cccaagccac
     1741 ctggtcggtc gtggcggcca accccatccg actcggagac gcggcgacct ggcgacggtg
     1801 cagttccaag tagagggcct caagaccgcc aaatgcgtct tgggaagggt gatcgacaga
     1861 caaggaaggc aacatgaccg cgagactatg gctccgagaa ggaggcaccc tcccgcaacg
     1921 cacaggtttg gcggaggtcc cgagccccta atctggtcga ggttcttcct tcgcagccga
     1981 aaggtctcgg atctcttcgt tcgactccac cgcgatcggt cgctttcgct tcctcggtcg
     2041 ctccttgagc cccgacggcc gggtcacgct gcgctacgcc ctcgacgacg tcgaggagtt
     2101 caccgagacg ttcgacatcc cgcttgagcc cggcgtaatc gctgacgagg acgccgtgcg
     2161 tccgcttctc gatctgctgc actgggtggc cggggtgagc tactacaagc tggcggcgcc
     2221 gccctccgtg agttgcgaga gtggagcacc tccccccgcg gcggcagcgt acctcgaagc
     2281 cttgtattca gaggggctcg gcgaattcgc ctacgcgaac cgcgatcggt tgccgcgact
     2341 gcccacgccc gagttcgccc ccggaccagc gctgcggccc acgggcggtg cagttcgcca
     2401 ggcccgttct ccaagacgcg ttcttgttcc ggtgggcggt ggaaaggact cggtcgtcgc
     2461 tcttgaggtc gtgaagcgat ccggagtcgg gtgcgcgctc tttgtcgtga actgcctcga
     2521 cccgatgcgg cgcaccgctg aggttgccgg cctgccgatg ctcgaggtgg cacgcagcct
     2581 gccgctcgcg acgttcaagc gcctccacgc ggcaggagcc ctcaacggcc acgtcccgat
     2641 cacggcgatc atctcgtgtg tagcgctgct cacggcgagg ctcaacggat acgacacggt
     2701 tgccctcgcc aacgagcgct cggcttcggt cgggaacctc atccacgacg gcgtcgaggt
     2761 gaaccaccag ttcagcaaga gcgcacgggc ggaaggcctg ctccgcgccg cagccgcgga
     2821 agcgtcaacc gatgtcgaca tctactcgct gctgcgcccg gcgtcggaac tggtcatagc
     2881 gcgcgccttc gcgacaaagc tcggcgccta ccaccacgcc ttcacgagtt gcaaccggaa
     2941 ctttcagacc gacccggccc tgcggggtgg cgaggcgtgg tgtcgtgact gcccgaagtg
     3001 ccggttcgta tacctgatgc tcgcgccgtt catggcgccg gaaccgctcg cagaagtgtt
     3061 cggcggcgcg atgctcgatg acgaacgaca atacgagggc tttgcgttgc tgaccgcgac
     3121 gggaggtcac aagccttttg aatgcgtcgg tgaggaggag gagagcctcg cggcaatcga
     3181 gctgttggac gcccgcgacg cgtggcgcga tcaggtggtg gtgcggcggc tcgtcgctga
     3241 agcgctagag gtgcacggcc gcgacagcac gcgaatcggt tcgtttctgc gactcgctga
     3301 ggagcacgcg atccccgccc aattagccga cgagctccgt gcgtttctcg gaccttgacg
     3361 gttgctctat cggcgtttgg ggggcgggcg ttgaaacccg ttcgttcgcg cgggtcctgc
     3421 gctcgtcgct aacgcgggca cggatcgcgg tcgtcgttct ggaggacgct ccggacgctc
     3481 ccgagttgac cgctgacgcg cgagtggtga gcgccagcga ggccttcgag gcgctcagaa
     3541 catgcgacgt gctcgtgcgc tcgccgggtg tttcgcgcca ccggtccgaa ctccgagcca
     3601 tcgccgcgca gggcacaccg atcgtcacgc cgaccgggct gtggctcgcc gagcgcggcg
     3661 gccgcaacac gatcggcatc accgcgacga agggcaagag cacgaccgcg tcactcgtag
     3721 cccacctggt cgctgcgacc ggccgaggag tgcagttggc cgggaacatc ggccgacccg
     3781 cacttgaatt gcttgactac cccgacaccg aactcgccgt cctggaactg tcgagctacc
     3841 agatcgctga cctcacctgc gggccgcagg tcgcgatggc cggcaacgtc taccgcgaac
     3901 acctggactg gcacctcacc gatgacgcct atcggcagga caagctacgc ctgctcgcgc
     3961 tgccgggtgt cgaacggtgc gtggtgaatg cgctgtcgcc ggaagtgatg gccgcggagc
     4021 gcgcgcccgg gccggtgtac gtctacggga ctgcaccagg ctggcgcgtc gacgacgacg
     4081 ggagcgtcat gaaggacgga gccccgtcgc tggcgcgcga cgtgctcccg ctgcgcggtc
     4141 ggcacaacgc gctgaacctc tgcggcgcgc tcaccgctct cgaggcgctc gacatcccgc
     4201 tgccgccgtt gccggcggcg ttcgccggct tgcaaccgct cgaacaccgg ttgcagacgg
     4261 tgcatgaggg cgacggcgtc gaatgggtgg acgacagcat ctcgacgaca ccggagagca
     4321 cgctcgcggc catggccgcc tttcccgacg cgcgcctgat cgtgattggc ggcggcttcg
     4381 atcgcgggca ggactacgcc gagctcggtc tcgtcgcggc ccggtcccgc gcgctggtcg
     4441 tgacgctgcc ggtcactggc tcacggctgg cccaggccgt ccgggcggcc ggcggggaag
     4501 cctacgaagt cgccgacctt ccggcggcag tcgcggcggc gcgcgccctg gcgacaccgg
     4561 ggacagtcgt gctcctgtcg ccggccgctc cgagcttcaa cacacacgtc gactatcgcg
     4621 cccgggggga tcacttccgg gaactcgccg ccactaccgg aggccccaac ccatgactcc
     4681 gcccgaaccc gccgtgctgt cacccgccgg ggacccgctg acctgggtcg acgcgtgggc
     4741 acgctctccc gacctggccc gggtcgtcgc ggccttcggg ggcgagacgt ccggtcagcc
     4801 ggatctcggg gcctggctcg cgtacctaga ggcgttctcg cagcgctggg actatcgcgc
     4861 cggccaggag cgcaaccttg tcaaggcccc cgacttcacg ccggacctca ccgcgatggt
     4921 cttcgccgct gccaccgtac tggggatgcg gggcaccacc gcgcctgcgc gcgaacacta
     4981 cgagcacgtc gtcatcctcg gggggctggt gcgggcctgc ctggcgcgtc cacgccacgc
     5041 ggcgaacctg ctagacgcgg gtacgatcac ggccgaccgc atcatcgcgc tgggtgggtt
     5101 ccgcccactc aagggggacg aggagacgct ggtcgccgag ttgctcgaca cctcggtgac
     5161 agacgagtac cacgcgatgc gtgccggggt agaggctgcg ttcggcctcg gagccccggt
     5221 cagcacgcgt ggcgagccat ccgaagtcgt cggcgcttcc tggggtgtca ccgactacgt
     5281 caccgccgca ggtgtggcgg cgcacgttgt cgctgcaccc tcgtcagagc cgggtgtccg
     5341 gcgcgccaac acgccggaca cgtacgcctg gctggcccgt gactcgacct ggctgcgccg
     5401 aggcgacgcg gtgctgctgg tcaccacgga catctacgcc ccgtatcagg gcgccgacgc
     5461 catccgcatg ctcgcggtgc cgcacggcgt cgaggtcgac gtcgtcggca tcgagcccgg
     5521 cgggatcgac ccgcgcttgg cacagccgtt tgcggtgcac aactaccttc aggagctgcg
     5581 ctccaccatc aggtcgctgc gcatgctgca ctccgtgctc accggctgat cccgcgccgc
     5641 ctccagcgtg atcactgcaa atccggacgt tcacggatgt ggcgccacgc tgacgctaag
     5701 atcgcgccgt gtcgagtccc cggtgtccgt gaaccgcgac tggccgggat tggtgctgcg
     5761 gctcgcgcaa gcgcgcggcg gggacgggca actctgccct cagacgtttg tcctgggcgg
     5821 gtcgcacgag acgcgcgtca cggtcgtggc gcagcaggcc cgggccatca acctcgcctg
     5881 ggcgctgcat cgcaccggac ggttggtcgg gcgcgacggg aaaccgagct ccgtcggggt
     5941 ggtcggggcg ggtgctgccg gggcgacgtt tgcggcggcc gcggggctgc tcggcgcgaa
     6001 cgtaacgttg gtcgacgagc acgaggaacc gatcaccacc cagcgctggt cattcgaccg
     6061 gtaccttcat ccgaacctct tcgattggcc ggtcgagggc tggaatcagg aacgtgctgg
     6121 cctgccattg atggactggg ccgcaggtcc aagcgcgcgt gtccggctcg aactgcttgc
     6181 cggcttcgca ctcgcggccg ctcgcggcgg cgtgacgtgg tgctcccagc actcggtgag
     6241 cgaggtcgac tccgccgacg gcaaggtgaa gctgaccgtc tgcgacctcc gtagcacagc
     6301 cgagccggat gccgcggggt ccgtgagagt ggcagaggag tcgtacgacc tggtcgtggt
     6361 ggcgaccggc ttcctgcccg aaccgcgggt gggcgaccgg acgaccatgt cgggcagcta
     6421 ctggaaggac aacgatctcg cgcaggtgct cgacgattct gagcagcgcc accacaccat
     6481 cgtcggtgac ggcgatggcg cgctgacgga gctactgcgt ctcagcctca tcggtcgcga
     6541 ggcgaagcgc gagttcaatc aatcgatgct gacaacgctt gcgacgcagc ttctcgatgc
     6601 cgaccgcctc agggaggacg tgctcagcgt cgaggccggc atcagcggca cgcgtgggaa
     6661 gggtacgctg cacgcgtacg cgagtggatc cttcgatggg gccgacgact ccttgcggga
     6721 ttggcttcga ccaagcgcgg ggggcgtcga agtccgcgcg ggccgcgtca cgtccctccg
     6781 caatgcgttc gcgctcaacc ggttcctcgc cgcccggctc actgcgggct ccagcgtgcg
     6841 tttccttcgc ggcgcgccgt tcctcgaagc ggacgtgccc gcactcgctc cgtctcgcgt
     6901 ggtgtggcga accggccccc gaccccgcgt ccggacgctg ctcgtccggc ccgcgctcgc
     6961 ggtgaaggac gcgggtgacg cactcgccgg tgacccgtct cgccaggatg ttctcggcac
     7021 cgtggacgac ctcatggacg cgagtcgcga gccgcgatgg gacgcgcgtg cgcgcgagtt
     7081 gctcgcagtc ccggcagaag aatcggcgct caaggcggcg gtggacctcg agcgcggcgg
     7141 tcttgccgtg tcgcgggggt ttgggtggaa gccggcgggt tggccggcgt cggtcgacgc
     7201 gccgccactg cccgagttgc cggacatgat cctcgatctc gcccggatgg cgctgtcaat
     7261 cggcaacggg ccaccgcctg aggtctggtc aaacttcctc gtcaaccacg acagcgtcgc
     7321 cgtctcgctc gatctggtcg cggtagctgc cgaccgcacg ccgaccgaga tcatcgatca
     7381 gctgcgccgg accgacgcgc ccgggatccg cctcgtgttg atacacgtcc cgcgcggccc
     7441 gagcgacctg gagcgggtat ggatccgtgt ggatcccgcg caggcggcgg cgcacctctc
     7501 gaagccgggt tggaccgcgg tgtcgttgtt gctcgcgccc gggttcgccg caggcgattc
     7561 cttcgacgac ggcgagacca ggaggttcga atcacacgcg gcgcctcgta cggaagactc
     7621 tgccttggac gtcttggcgg agcgactgct gaatgagcgc gctatcgcat cgacgcagtg
     7681 ggtcacggcg gaccggatca cggcggcggt cgccgagcgg agcagcgagc aaacactgtt
     7741 gcaggccgcg cgcgaaggtg acgcgcggct ggagcgggcc ctggtgaccg tgctccaccg
     7801 gcggcttggt gacggtgacc cctatgagtt ccggtcggcg ctgcttgggc tcgcggccct
     7861 ggtcggcacg gacgacgatc ggcccgggtc ggtcaccgac gtgtacctcg ctacggtcct
     7921 cgcgctgccg gccggtgatc gggcgggtgc gctggtccgg gccgcgctgg ggccgttggt
     7981 ccgcctcgat cccgctactg gcgagctggt cccgctcagt cggtacgacg accgcctacg
     8041 cagcgagtcg ccagaggtgc tggctgcaca cgacggccct cctcacgacg gcgctcctca
     8101 cggccgcggc gtccccgact tttcggactt tgcggtccgt gtctcctcca ctgataccga
     8161 gcgtgccgaa gatgcctgat ccgccgacca cctcttggcc gctttggcaa cgcgggcgcg
     8221 ccgcgaagac cgaggacgcc gagtatgtcg ccctcgcccg ctatgcgacg accgacgcgc
     8281 tctggggatt cgacgccccg gagctccccg ctggcctgtc cgcgccggag atcgtggcgc
     8341 gcgtgtacgg cgaggtactc gcaggcgcgg gctggaacta cgttgaggag cccaagcggc
     8401 ccgcgccgct gtcgtcgctc gcgtcgcagc agatccgcac gccgcgcgac atccagcgct
     8461 accgggcagc cacctgcctt gacgcaagcc tgttgctgac cgcgcttgcc gtgcgcgccg
     8521 gaacgcgccc gcacctggcg gtaggccgaa accatgcgtt cgtcatcgtc ggcgaccatg
     8581 tgggcggcac cgccaccggc accggcggca tcgcctacgc gagccgggct accgccgacc
     8641 tcgtcggcga cggggtggcc atggccatcg acgtcgtcca ggcgctcgtg acaccgaagc
     8701 gtgcgcaggc ggcgagcttt gccgacgccc ttgcggaggg ccagcgatgg gtcgctgacg
     8761 ccgtcacctc gggacaacgg ctgatgctga tcgatgtggt cgccgcgaac gagcggcacg
     8821 ggttcacccc gtacgcgccg gaccgggacc cgaccttcat ccccaggagc ctcccggcac
     8881 acgccggcgc gctgaagcta tacgagagtc aggaaagcat ctgcgagcgg ctgcgcggca
     8941 agcacggcgt catcgtcatc cacggcgact cggggagcgg caagaccaca gccgcgctgc
     9001 atctggcggc ggagcgcgag tcgggaccgg ccgtcattct cgatgcttcg tcgggtgaga
     9061 cgatcgtcac gtcggcggcc gaggttggtg ctcgtgaacg cggtgtgtcc gtcgggcgcc
     9121 tggagccgca ggagcgggac ggcgacgcag cgtcgctact tgcccgcctg agcacgtcgc
     9181 ccggttcctg gtgcgtggtg ttcgacaacg ccgacgggga tccgtcgcag gtcctccgtt
     9241 ggctgccgcg ccctgatcgt gagaaggggc aggcggtgat cgtcacgacg accaacccga
     9301 cctggctgca actcagcctc gtcaacgagc gagaggcgct cgcaccgatc gatgacaccg
     9361 acgtcgcgcg cgccctgggc actcactccg cgttggcgga gctggtcgcc ggacgtgcgg
     9421 tgacgctgcg cgcattcctg cgctatctga gcgacggcgg gggcgatgcc gcgcgcctgc
     9481 agcgtcacgc ggaggcccta ccggacgacg agcttcgtgg tccggtcgca ctcgtcagcg
     9541 ccgtgctcga ggagtacggc gaactggggc cggtccttca gcgccttgag ctgctgccgc
     9601 ctgaccacct gccgatttgc gacgacgtcg ccgactacgc tacgttccag cggctcgagg
     9661 cgctcgggct cgtcgacctg acgccggaag ccggtgtggc gcggatgcat cgcctggtcg
     9721 gtcgcgccgc cgagcgcgtg ctgggccaag acgacgaggc gcgcaccgcc gcggacatgg
     9781 tgcgcggcgc cgagaccttc gccgcgctct ccgccgccgc ggaaccgagc ctcctggagc
     9841 gctacaccaa gctgatcctc gccgcattcg cggcgaggca gcccacgttc ctgatcgacg
     9901 ggttcgtcct gcagcgggcg gcgagcctca tggagctgcg cggccgcgtc gaagagtcgc
     9961 ggcaactcgc cgagccggcg gtgacggtac ttggggcggt ggccggcgcc gagccgatcg
    10021 aactggcaga cgcgctccag agccttgcgc gatggacgaa ccagcacgac ccgcggaacg
    10081 ccgtcgcgct ggccgcagcg ctcgagcaga tggcgctagc gcaaagtgtg gccgctcgct
    10141 tcggcgatga gcggcgtgcc gcgcgctccc gccggcttga agccttgctg aggctcaagg
    10201 tggggtggct caacgcggat cttgagaccg ggacgcgcga catcgtcgcg gcgctcggcg
    10261 acctcgagcg cgaggcggac gccgccgagc ggccggacag cggtgtcgac cgtggcgagg
    10321 cactgcgcgc tcgcttcaac gtcgccggcc cacgcgtcac gctggcgcag cgggtcccgt
    10381 cagaggcgga cgggcacctg cgggaggcac aacgggtcta tgaggtcgtc ggccacaggc
    10441 gtcaggcgct gtacccgcag acgcagcatc cccacgtcgc ggcgtgcatc agcggcgaag
    10501 cgctggcccg gtacatgcgc gcgaacctcg tcgccgtcac atacgatgag cgccagcgat
    10561 tgctcgctga ggcgaccgac ctcgagcgcg aagcgttgac cgaccgcttc gaactggagg
    10621 gcccgctcgg cggcgcggac acgagcaagt cgtcgaagct gctcacgaag atctcctacg
    10681 cacggcatcg cctgaacgac agcgcagtgt cgagcacggt gctcgacgag gcccgcggcg
    10741 agatcgcggt cccccggctg accgccgcta ctgcggatca ccgtgcgtac gccgagcgct
    10801 ggatgtccgc accgcagctt gcggccgtgc tgcaggtctt cggagtcagc acgcccgcgg
    10861 cgcttgaacc ggtcgccgcg gagcggacga tctacgacgt gctcgaaacg cgcacaatcc
    10921 tgtccgcggc acgcgctctc gggctgatgg acgtcgcgct acccgtccgg cgcgtgtacg
    10981 accagatcgt cgtcctcgac ggcgacggtc gcttcgcgga gcgggctgcc ggcgtcgtgg
    11041 agcggctggt ctctggcggt gaggtc
//
LOCUS       DASVEU010000200         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1302578,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000200 DASVEU010000000
VERSION     DASVEU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302578"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF225_08285"
     CDS             <1..197
                     /locus_tag="VF225_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145293.1"
                     /translation="NIGKYSPGIVTERHYFFTLNGIVMAWHGRRSPQILALQRAKKRQ
                     ATQKKSAQGRVTTAPKGGAP"
     gene            complement(150..1109)
                     /locus_tag="VF225_08290"
     CDS             complement(150..1109)
                     /locus_tag="VF225_08290"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX7145294.1"
                     /translation="MTATPGGPPVLVLGVRRSGTTLLRVMLDRHSELAVPDESYFVPQ
                     LADRHFRHVEPDEFVDDLRRIDTLAEWNVPLDKVRARLTERMPIGAAIATVYAVYATE
                     RGKARWGDKTPMYMQNLRLLARLFPDALFVHLIRDGRDAALSFLAMPRGLMTETWMQP
                     RDTAGFACQWRTEVKAARRLGRSVGVERYLEVRYEDLVRDVESVLRRISEFARLPYEP
                     AMADYVGNVDVSAKPHQQSLRHPLTAGLRDWRVQMSPEDVGAFEQVAGDLLDELGYET
                     HERPDAGGRFKRASYNARAAAWRAASLGLRRSPLWRRRHPALR"
     gene            complement(1106..1795)
                     /locus_tag="VF225_08295"
     CDS             complement(1106..1795)
                     /locus_tag="VF225_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7145295.1"
                     /translation="MRRNAPTILALLLIVATATAFAVTQRLKLEPSPISQTRVSDVFS
                     PVCRCASRAASIEFSLRRADDVRIAVRTGSGEVTVAEGSFAQGDVHVRWDGRDASGEL
                     VPDGTYHPFVELKRAGRTIDLPNPIRVDTIRPSIKFTSLRPRLFRPGAEKLKVTYRVS
                     EHAHALLFVDGRRRVFTASTRLRGRLQWYGKVRGHLIRPGRHKVKLVAEDLAGNRSAP
                     TPWVVVRVRRP"
     gene            complement(1701..2669)
                     /locus_tag="VF225_08300"
     CDS             complement(1701..2669)
                     /locus_tag="VF225_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7145296.1"
                     /translation="MTWQFTLTGLLIGVLVGLTGMGGGALLTPILVLVFGFKPTYAVG
                     TDILHGAVFKSFGAVRHRRLGNVHAHLTLWMFAGSGPLSLLGVAVAGRLERSYGDGVQ
                     SAEGYAIGAALVLGAIGLVAKTFIKRGMQPSEAGFVMQRKDKFRAVAIGAVFGFIVGL
                     TSVGSGTFFGLVMVMVYPLTIARIVGTDIFHAAALLWVAGLGHLLQGNVDLHAMGWLL
                     LGSIPGIWISSRFTLQIPQDALRLGLAGVLALSGVKLLNVPAATYVISIGLGVMLTVF
                     VVWLATGMRGVQSRSDEAQRTDDSRAPAHRGDRDGIRGDAAPEARA"
     gene            complement(2673..3815)
                     /gene="sat"
                     /locus_tag="VF225_08305"
     CDS             complement(2673..3815)
                     /gene="sat"
                     /locus_tag="VF225_08305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX7145297.1"
                     /translation="MSVIADTKLIAPHGGTLVDRTGDRPGDVETLEVVTLTSRELSDL
                     DMIASGALSPLEGFMGREDCEAVLDTMRLANGLPWALPVCLAVDADATPEGDRVALAD
                     ESGRRFAVLEVEESYEYDKQHEAQQAFRTTDEAHPGVARLYEQKPLYLAGRVTVFERA
                     EPSFPELAKDPAETRAEFAERGWRRVVGFQTRNPIHRAHEYLTKVALETVDGLLVHPL
                     VGDTKSDDVPAATRVECYRVLLDGYYPPDRFVLSSFPAAMRYAGPREAIWHAICRKNY
                     GCSHFIVGRDHAGVGDYYGTYDAQLIFDEFEPHELDIEPMFFEHSFWCKACGSMASAK
                     TCPHGGDDHVFLSGTKVREMLEAGELPPQEFSRPEVAEVLIQAYRA"
BASE COUNT          699 a         1360 c         1320 g          604 t
ORIGIN      
        1 tgaacatcgg caagtattcg ccgggaatcg tcaccgagcg gcattacttc ttcacgctga
       61 acggaatcgt gatggcgtgg cacggacgac gaagcccgca gatcctcgcc cttcagcgcg
      121 ccaagaagcg gcaggcgacg cagaagaagt cagcgcaggg ccgggtgacg acggcgccaa
      181 aggggggagc gccgtagacc gaggctggcc gcccgccaag ccgcggcgcg cgcgttgtac
      241 gacgctcgct tgaaccttcc gcctgcgtct ggccgctcgt gcgtctcgta gccgagctcg
      301 tcgaggagat caccggccac ctgctcgaag gcaccgacgt cctcgggaga catctgcacc
      361 cgccagtcgc gcaggcccgc ggtcagagga tgccggaggc tctgctggtg cggcttggcc
      421 gagacgtcga cgttgcccac gtagtcggcc atggccggct cgtacggaag ccgggcgaac
      481 tcagagatgc gccgaagcac cgactcgacg tcgcggacga ggtcttcgta gcgcacctcg
      541 aggtagcgct caactccgac gctgcggcca aggcgccgcg ctgccttcac ctcggtacgc
      601 cactggcacg cgaatccggc cgtgtcgcgc ggctgcatcc acgtctccgt catcaagccc
      661 ctcggcatcg cgaggaacga cagtgccgcg tcgcgcccgt cgcgaatgag gtggacgaaa
      721 agcgcgtccg gaaacagccg cgcgagcagg cgcaggttct gcatgtacat cggggtcttg
      781 tcgccccaac gcgccttgcc acgctcggtc gcgtagaccg cgtacacggt cgcgatggcg
      841 gcaccgatcg gcatccgctc ggtcaggcgt gcgcgcacct tgtccaacgg cacgttccat
      901 tccgcaagcg tgtcgatcct gcgcaggtcg tcgacgaact cgtcgggctc gacgtgccgg
      961 aagtgacggt cggcaagctg tggcacgaag tacgactcgt cggggacggc gagctcggag
     1021 tgccggtcga gcatcacccg gagcagcgtc gttcccgagc ggcgcacccc gaggacgagc
     1081 acgggtggac cgccgggggt cgccgtcacg gacgacggac gcgcacgacc acccagggtg
     1141 tcggcgcaga acgatttccg gcgagatcct cggcgacgag cttcaccttg tgtcggcccg
     1201 gccggatcag gtgaccgcgc accttcccgt accactgcaa ccgccctcgc aggcgcgtgg
     1261 acgctgtgaa cacgcgacgc ctgccgtcga cgaagaggag cgcatgcgcg tgctcggaga
     1321 ccctgtaggt caccttcaat ttctccgcgc ccggccggaa tagcctcggc cggagacttg
     1381 tgaacttgat cgacggtctg atcgtgtcga cgcggatcgg gttcggcaga tcgatcgtcc
     1441 tgccggcgcg cttgagctcc acgaatgggt gatacgtccc gtctggcacg agctccccgg
     1501 aagcatcccg cccatcccag cgaacgtgca cgtcgccctg cgcaaacgag ccctcggcga
     1561 cggtcacctc gccggaaccg gtccggaccg cgatccgcac gtcgtcggcc ctgcgcagcg
     1621 agaactcgat gctcgctgcc ctggacgcgc agcggcacac gggcgagaag acgtcggaga
     1681 cacgggtctg ggagatcggg ctaggctcga gcttcaggcg ctgcgtcacc gcgaatgccg
     1741 tcgcggtcgc cacgatgagc aggagcgcga gaatcgtcgg tgcgttgcgc ctcatcggag
     1801 cgcgattgta cgccccgcat cccggtcgcc agccagacga cgaacacggt gagcatgacg
     1861 ccgaggccga tcgagatcac gtaggtcgct gccggcacgt tgagaagctt gaccccgctg
     1921 agtgcgagca cgccggcgag gccgagccgg agcgcgtcct gagggatctg cagcgtgaac
     1981 cggctcgaga tccagatccc cggtatggag ccgagcaaca gccagcccat cgcgtgcaga
     2041 tcgacgttgc cctgcaggag atggcccaag ccggcaaccc agagcaacgc ggcagcatgg
     2101 aagatgtccg tgccgacgat ccgagcaatg gtgagcggat agaccatcac catgacgaga
     2161 ccgaagaacg tgccacttcc gaccgaggtg aggccgacga tgaagccgaa gacggcaccg
     2221 attgcaaccg cgcggaactt gtccttgcgc tgcatgacga atccggcttc gctcggctgc
     2281 atcccacgct tgatgaatgt cttcgcaacg agaccgatgg caccgagcac gagcgccgcg
     2341 ccgatcgcat agccctcggc cgactggact ccgtcgccgt aggagcgctc gagccgtccg
     2401 gcgacggcca ctccgagcag ggacagcggc cccgaccccg cgaacatcca gagggtgaga
     2461 tgagcgtgga cgttgccgag ccgccggtgc cgcaccgcgc cgaagctctt gaagaccgcg
     2521 ccgtggagga tgtctgtccc gaccgcgtac gtcggcttga agccgaagac aagcacgagg
     2581 atcggtgtca gcagcgcgcc gccgcccatc ccggtcaggc cgacaaggac gccgatcagc
     2641 agtcccgtga gagtgaactg ccaggtcaca cgctacgccc ggtacgcctg gatcagcacc
     2701 tccgcgacct cgggccgcga gaactcctgg ggcggcaact cacccgcctc gagcatctcc
     2761 ctcactttgg tgcccgagag aaaaacgtgg tcgtccccac cgtgtggaca ggtcttcgcc
     2821 gacgccattg acccgcacgc cttgcaccag aaggaatgct cgaagaacat cggttcgata
     2881 tcgagctcgt gcggctcgaa ctcgtcgaag atgagctgtg cgtcgtacgt gccgtagtag
     2941 tcgccaacgc ccgcgtgatc gcggccgacg atgaagtgcg agcagccgta gttcttgcgg
     3001 cagatcgcgt gccagattgc ctcgcgcggc ccggcgtagc gcatcgcagc gggaaacgag
     3061 ctgaggacga aacggtccgg cgggtagtac ccgtcgagta gcacgcggta gcactcgaca
     3121 cgcgtggccg cgggcacgtc gtccgacttc gtgtctccga cgagcggatg gacgagcagg
     3181 ccgtcgacgg tctcgagcgc gaccttcgtc agatactcgt gtgcgcggtg gatcgggttc
     3241 cgcgtctgga agcccacgac gcggcgccag ccccgctccg cgaactccgc ccgcgtctct
     3301 gcgggatcct tcgcgagctc ggggaaggac ggctcggcgc gctcgaacac ggtcacgcgg
     3361 cccgccaagt acagcggctt ctgctcgtag aggcgcgcga cgccgggatg agcctcgtct
     3421 gtcgtgcgaa acgcctgctg agcctcgtgc tgcttgtcgt actcgtagga ctcctcgacc
     3481 tcgagcaccg caaagcgccg gccgctctcg tccgcgagtg cgacccggtc accctcgggc
     3541 gtggcatcgg catcgactgc caggcagacc ggtagcgccc acggcagtcc gtttgcgagg
     3601 cgcatcgtgt ccagcaccgc ttcgcagtcc tcgcggccca tgaagccctc gagcggcgac
     3661 aacgcacctg acgcgatcat gtccagatcg gagagctcgc gcgaggtcaa cgtcacgacc
     3721 tcgagcgtct cgacgtcgcc gggccggtcg cccgttcgat cgaccagcgt gccgccgtga
     3781 ggcgcgatca gcttcgtgtc ggcaatgacg ctcatgccgt gactccggcg ggtaccagtc
     3841 cgcgctcctc gaggtgctgc aggatcagct gcgcgctctc ctcgggctcg tgctgctcgg
     3901 tgtcgatgcg cacgtctgga ttgctcggct cctcgtacgg atcgtcgacg cccgtgaagc
     3961 ccttgatctc gcccttgaac gcc
//
LOCUS       DASVEU010000201        16259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_915414,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000201 DASVEU010000000
VERSION     DASVEU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915414"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF225_08310"
     CDS             <1..789
                     /locus_tag="VF225_08310"
                     /inference="COORDINATES: protein motif:HMM:NF027552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HEX7145298.1"
                     /translation="TVYSGLVHVGIARSLFAPTLRGDIALARSDRPGLRVLFVGNSFT
                     FKNSLPALVRELAAGDPAGKPIYSVEYAAPGWSLGQADKDGGLTKLLRDVKWDVVVLQ
                     EKSWLLSFPEEQWRRSTYPFARDLQGKIAASGAQTLLFMTWGYALGDRRTIPHDTYAE
                     MQRRLAEGYSKLGAEVGARVAPAGLAWAEALRRKPALDLWAADGQHPGRKGSYLAACV
                     FYAALSGRDPTRSRFTAGIEAGQARFLQRVAESVYESDVRSGSA"
     gene            875..2167
                     /locus_tag="VF225_08315"
     CDS             875..2167
                     /locus_tag="VF225_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX7145299.1"
                     /translation="MTARASAGPGAALAAPAWIDRWLAACHDLDELGRSHPMSDAVIE
                     EIDGRRIRVGDKWLADFASCNYLGFDLEREIIEAVPAYLDKWGTHPSWSRLLGSPVLY
                     SQIEERLTALVGAQDTLVLPTITLIHMSVIPVLAGSGTIYVESRAHKTIYEGCQLAGA
                     RGATVKRFLFEDAEDLERLLKENGPSPRLICVDGVNSMTGNAVDLPAFARLAREYDAL
                     LYVDDAHGFGVIGERSDDELSPYGNRGNSIVRYAGETYDNVVLVSGLSKAYSSLAAFL
                     TCPPELKRLLKTAAPPYLYSGPSPIASLATVLVGLDVNEKRGDVIRADLWRKTAAVLH
                     ELDRLGVHTPNRSGFPIIEVPLARHEDIDAVGRFLFDNGIYVTLAAYPLVPRSEVGFR
                     VQVTAANTDAEIEQLIDVVGRLVNVFDLQPARTSERAA"
     gene            2164..5313
                     /locus_tag="VF225_08320"
     CDS             2164..5313
                     /locus_tag="VF225_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7145300.1"
                     /translation="MKRFALPGTPRDQVWIWYLAGTGVLTSLYLFAPALAGNGPLINA
                     LGLSGVVAIVVGIRMHKPRARAAWWLFAAGQFLFFSGDLYTYSYPKLLGADVGFPSIG
                     DALYLLVYPVLMAGLFILIKRRNPRPDRTALIDALILTIGVGLLSWVFLIAPNIHLTG
                     LTVLANAVSVAYPLGDVLLLAAAIRLAVDAGKRTPAFWFLVGSIVCLMATDSAYNYAL
                     LKDTYNHQLIFDAGWILYYLLWGAAALHPSMRSLEEGAPELGTRLTPLRLTLLGSACL
                     IAPGIRFVQSIGNPDVLVLIIASAVLFLLVVARMAGLVRQEARVVSRERALRGAGVEL
                     VAAAGHEQVANAAISAVHRLLAAEPPVRLVLVSEGVAVVEASSDGATGGRVGEGTCDW
                     LLQERSGSLRVLHAEPPPYVRGDLRLPEGQTTIVAPLTVRGEVRGAIVISSPVSLTRD
                     LIDALGALATQVSLAVEGASLAADLHRRQSEARFRSLVAHSSDLITVLDADGLITYQS
                     PSIERLLGYRVDEVEGTRFDRLLSETDRPLLAQLIAIDGSGDAEAHTIECSVSHRDGA
                     TLRFEVQHTDLLHDEHVRGIVLNCRDVSERKAFEEQLAHQAFHDPVTKLANRALFSDR
                     VEHALMRTQRGVHEIAVMFIDLDDFKTVNDSLGHAAGDEVLQEVGRRLKIAVRPGDTV
                     ARFGGDEFAVLLDGVSGSSDAADAAARILRALDLPIEIEGKNVFPRASVGICLVGEDL
                     EAPEASELLRNADVAMYMAKRDSKGSYRVFEPTMHERVVERLELRSDLQHALTMDQLE
                     LHYQPVVRLVDREILGVEALVRWNHPTRGTIPPIQFIPVAEETGLIIPMGRWILETAC
                     REGARLRETFPRTEGLTMSVNLSVRQLQSETLVADVRQALEVTGLPAAALVLEITESL
                     MLTDTDFAMMQLHELKALGIRLAMDDFGTGYSSLSYLSRFPVDILKMDRSFVGAEDNS
                     TLTSAIIALGTSLSLEVVAEGIELPEQASSLEELGCELGQGFLFARPMDSDALTKFLT
                     VAGASTQEDVESSAASNAA"
     gene            5303..6610
                     /locus_tag="VF225_08325"
     CDS             5303..6610
                     /locus_tag="VF225_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145301.1"
                     /translation="MQHSHEALDRPGGFGRGNLLSPLKHRDFRILWTGMTISLIGDGI
                     FLIAIAWESYSLWNAPAALSIVGIGMTIPTIAFLLIGGVVSDRRDRRVVMAWADGLRA
                     VAVAVLAALVLMGALRFWELVALVAVYGIGTAFFMPAFEAILPELLPKSELPAANALD
                     QFVRPIAMRLIGPVAGGALVAASAGVAFAIDAASFAVCLIAVLAMPRRAGRHVEVQTS
                     SVAALKEGLRFVRQRVWLWGTLLSAAIAYLVFLGPAEVLLPFLVKNELHASAGTLGLV
                     LAAGGLGAVGGAAFMGHIGHPRRDVTVMYMTWTFATLAIAGYGIANAAWELMLVCLVF
                     NALEAAGTIVWATIKQHHVPGSMLGRVSSLDWLISIGLLPLSFALTAPVAAVFGARAT
                     LVGAAALGAAVTLSAFFLPGMRAIERTGGRRQGPWHVPVDATD"
     gene            6647..7543
                     /locus_tag="VF225_08330"
     CDS             6647..7543
                     /locus_tag="VF225_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7145302.1"
                     /translation="MTDRRALTIALVLVVLLMAAEIAAGLIGHSLALLADAGHLLTDA
                     AALAFALVASAMAARPAAGRWTFGYSRLEILAAQANGITLGLLALWVIWSAGHRLADP
                     RDVRGGLVLAVALGGVAVSLAASFVLARASRESLNVRGAFLHVLTDVAAFGAAALAGG
                     LILATGWDRFDPIASLAVAALMLWSSVQLLRESTLIFLERAPGDADPEAIGRALVAEQ
                     DVVEVHDLHVWTVTSGFPALSAHVLVTPGADCHAARRRLELILSERFGLTHTTLQVEH
                     AEPASRTVELGEAVARHGPLGR"
     gene            7553..8290
                     /locus_tag="VF225_08335"
     CDS             7553..8290
                     /locus_tag="VF225_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7145303.1"
                     /translation="MPRLEGKSAVVTGASSGIGAATALALAGEGARVVGGARRVERIQ
                     APLTPLGLDVTDPESCERFVKAATEELAGVDILINAAGLALGRDLFDESTEEDERTVL
                     ETNVNGLIRMTRLFLPHIRDGGHIVNIGSIAGRQAYEKAAVYVASKFAVRGFTYALRE
                     DLLGRPIHITTVDAGLVESEFSRVRFRGDAETARKVYEGVEPMTPQDVADCILFAVTR
                     PAHMNVDEIVIKALAQSSGGRIVRTET"
     gene            8290..10362
                     /locus_tag="VF225_08340"
     CDS             8290..10362
                     /locus_tag="VF225_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX7145304.1"
                     /translation="MPGQLRILEGATFCICDEVGDLHHQGEGLFSEDTRYLSRFKLTI
                     NGNRPLLLSSDKIEYFSAAFFMRNPLAGGLDQDVLSIRRERFVGEGMQDSFSVQNQGM
                     KPVEFDLELEVATDFADIFAVKDYDFALGDPLRAKPLPELRPNEYDEERNQFLISDNG
                     DAPVKTQVIFSQLGEVDGSTVKYRLALQPRERWNLDVEVIPLPDGELVAPRFAHRRFG
                     EEVGRVRDSLAAWQLRVPQLRTSWDALGHSFGQSVSDLASLRMQSDGAGIGKLPGAGV
                     PWFMTVFGRDTIITCLQTLVFGPELARTALGVLADLQAKEDDPTIDAEPGKIVHEVRH
                     GKAARAWHERYYGTADATPLYLILLSEVWRWTDDAALVRDLKEPAMLALEWIDKYGDR
                     DGDGFVEYERRTERGLENQSWKDSWDSQRFHDGSLAKTPIAPCEVQGYVYDAKQRIAE
                     LAREVWRERELATRLEQEAEELRRRFDDAFWVEERGGYYALALDRDKRKVDSLCSNIG
                     HLLWSGIVPPHRVDAVVDQLMGDELWSGWGVRTMSTGDAGYNPLSYHNGTVWPHDNSL
                     IAAGLARYARWPEAQRITQRMLTAAQHFNYQLPEVFAGMRRSETPFPIAYPTAARPQA
                     WAAGTPVLLLQLLLGLQPDRRRHALETVAPLELPSWAGTIRLSGIRAFDRLWDARIDD
                     GKVRVEEA"
     gene            10373..11401
                     /locus_tag="VF225_08345"
     CDS             10373..11401
                     /locus_tag="VF225_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145305.1"
                     /translation="MNIAILAPVWFAVPPIGYGGIEWIVWLLADGLVDAGHDVTLFAS
                     GDSRTKAKLAAVFPEAPSRQIGRTYWELNHALSCFGRADEFDVINDHTGMLGATLGAT
                     TSTPVVHTVHGPLDGEPGLIYEKISRVAPQVGLISISMNQREPKPDLNWIANCPNALD
                     FSVYPVKPHRGEYLLFLGRMSPDKGAHRAVAVAMETGLPLKLAGKMQEPKEREYFHEL
                     VEPHLVDGIEYLGEVTHGEKVELLQNARATLFPIEWEEPFGLVMIESMACGTPVIATR
                     WGAVPEVIEDGRSGIIVDHYKQMAGALPAADDLDPFELRRYVEEEFSPERMVRDYVAA
                     YEKAIQGH"
     gene            complement(11398..11808)
                     /locus_tag="VF225_08350"
     CDS             complement(11398..11808)
                     /locus_tag="VF225_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX7145306.1"
                     /translation="MPTVPVPPDVDEFLLRANPAVVATLRTDGSPHTVPTWYDWESGR
                     VLLNMDESRLRLRFLRRDPRAALTVLDGRSWYSHISLIGRVVSIEDDPELVDIDRLAV
                     RYTGQPFRRREAKRVSAWLEPERWHGWSDAGPWG"
     gene            complement(11819..13075)
                     /locus_tag="VF225_08355"
     CDS             complement(11819..13075)
                     /locus_tag="VF225_08355"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145307.1"
                     /translation="MLVELGPLRVRNFRLLAGARTISFFGTNLAPIAVAFAVLDLTGS
                     ATDVGIAFACWTLAQVSTLLIGGVVADRLPRRLVMLSSDSANFAIRAAMGALLVSGNA
                     RIWEIFVLQALGGAATAFYSPASTGLVPETVRPALLQQANAFMSISRYLAFPVGAAVG
                     GTIVATVGSGYALLVDAATYATSALLLSRIRLPDRARTAASPNFLRELREGWQAFTEH
                     TWVWLLTGWISLYFLVTYAPFFVLGPYIAKNSLGGATAWAIIVTGEAIGALAGGLLGL
                     RLRPRRAMTSIGALFITTALQCALLAIHAPALAIGAAAVLAGFAFSYGTVIWDTALQQ
                     TIAPEKLARVSAYNWMGAMAFLPAGYAIAGPVADVIGMSTSLWIGVVWIVVTTLIVIS
                     VRDVRNFRLEPVGLSEAELAPAVAAS"
     gene            complement(13065..13916)
                     /locus_tag="VF225_08360"
     CDS             complement(13065..13916)
                     /locus_tag="VF225_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7145308.1"
                     /translation="MSPDPLDLHFQGAQRVIGSYVLETKDGPALYDCGPSSCIDRLHS
                     GLRQRGLELQDVRHLLLSHIHLDHAGAAGVLVREHPELQVHVSEIGAPHLIDPSRLEA
                     SARRLYGDTFDALWGELAPVPTENIHVVGPDVLGLDCFPSPGHASHHVCYLAGDGTLY
                     AGDAAGVRLQPDRTVLPPTPPPDFDLAAWERTIDEIERRKPSRLALVHFGVAADPESH
                     FAELRQRLGDWTRRVEQGVTEEEFSESARSDVTATGGDVETYERAMPFWQSYAGLKRY
                     WEQRRAR"
     gene            complement(13939..14529)
                     /locus_tag="VF225_08365"
     CDS             complement(13939..14529)
                     /locus_tag="VF225_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145309.1"
                     /translation="MRRPLLLAAALTAVLLFPAAATAKGPSAASVTGPGLRHAVNIEG
                     YGEGGNDTALGILVSDGGFFSQAFGATPSATTSRPLGGLGPRYEVTYTVPGGPNGDST
                     LHQDLYPYAVKGVVTYMAPAQRFWDTQSTPGGWYQGTAALRGTLVKAGLPPRAPARKT
                     RAERKLGVAVGAGAGVALAAGALALFRRKRGRSTSV"
     gene            complement(14556..15716)
                     /locus_tag="VF225_08370"
     CDS             complement(14556..15716)
                     /locus_tag="VF225_08370"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX7145310.1"
                     /translation="MDFETRAIHEGQEPDPATGALTTPIYLTSTYVQDAVGEHKGYDY
                     SRVANPTRTALQQCLASLESADHGVAFASGLGAVSTIMHLLSPGDRMVLIADVYGGVY
                     RMTSQVYEPKGYVFDYLPAAEFDDLASHLDERTRIVWIETPSNPLLNIVDIEKAAEAA
                     HAAGALLVVDNTFATPYLQRPLELGADVVVHSTTKYLGGHSDVVGGFAATNDPTIAER
                     LLFLQKSLGAVPSPLDCWLVLRGIKTLAVRMRQHCRNAHALSGFLERHPRVEQTLYPG
                     LPRHPGHAIAAKQMHDFGGMISFLVESEEEATAFVASTKIFKLAESLGGVESLIELPA
                     RMTHASTADAPFAAPRNLIRLSVGLESADDLLEDLEAAFAKVVSGTHSARSV"
     gene            complement(15717..>16259)
                     /locus_tag="VF225_08375"
     CDS             complement(15717..>16259)
                     /locus_tag="VF225_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX7145311.1"
                     /translation="KVTLMLELLEGRGVVAIVGRRGKQRLWDLAERWYPEAESIPWPE
                     AKQLLAEKSFRALGVRFENGEWQAHPDAEDGPMPKRVTFLSPFDRLVHDRDRAEALWG
                     FRYRLEMYVPKPKREYGYYVLPILRGDRLIGRIDPVLDRKTGVLHVNGIWWEPGVKPV
                     SVQKPLRSLATFIGAGSIET"
BASE COUNT         2621 a         5417 c         5477 g         2744 t
ORIGIN      
        1 accgtctact ccgggctcgt ccacgtcgga atcgcacgct ctctgtttgc cccgaccctg
       61 cggggggaca tcgcgcttgc gcgcagcgac cggccgggcc tgcgcgtgct gttcgtcggc
      121 aacagcttca ccttcaagaa ctcgctgccg gcgctcgtac gcgagctcgc ggcgggcgat
      181 cccgcaggaa agccgatcta ttccgtcgag tacgccgcac ccggatggag cctgggccag
      241 gctgacaagg acggcggtct gacgaagctt ctgcgcgacg tcaagtggga cgtcgtcgtc
      301 ctccaggaga agagttggct gctctcgttt ccggaggagc agtggcgtcg cagtacctat
      361 ccgttcgcgc gcgacctgca ggggaagatc gccgcgtccg gcgcccagac cctcctcttc
      421 atgacgtggg gctacgcgct cggtgaccgc aggacgatcc cgcatgacac gtacgccgag
      481 atgcaaagaa ggctggcgga gggctactcc aagcttggag ccgaggtagg tgcccgggtc
      541 gcgcccgccg ggctcgcgtg ggcggaggcg cttcggcgca aaccggctct cgatctctgg
      601 gcggctgacg gccagcatcc cggccggaag ggttcctacc ttgcagcgtg cgtgttctac
      661 gcagcgctca gcggacgtga cccgactcga agccgtttca cggccggcat cgaggctggg
      721 caggctcgct ttctccagcg ggtcgcggaa agcgtgtacg agtcggacgt caggagcggc
      781 tccgcctagt ctcccggcgg gcgtgttgac tcctgttgtc ggagtcctca cccggaccct
      841 caagatcagg ccggatccgg ccgatggtgc ctgagtgact gcgcgcgcat ccgccggccc
      901 cggggccgcg ttggcggctc ctgcttggat cgacaggtgg ctggcagcct gccatgacct
      961 cgacgagctc gggcgctccc acccgatgag cgatgcggtg atcgaggaga tcgatggtcg
     1021 caggattcgc gtcggagaca agtggctcgc ggacttcgct tcctgtaact acctgggctt
     1081 cgacctcgag cgggagatca tcgaagccgt cccggcgtat ctggacaagt gggggacgca
     1141 cccgagctgg tcgcgcctgc ttggcagtcc ggtgctctat tcgcagatcg aggaacgtct
     1201 caccgcactc gtaggcgcac aggacaccct cgtgctgccg acgatcacgc tgatccacat
     1261 gtcggtgatt ccggtgctcg cgggcagcgg cacgatctac gtcgaatccc gggcgcacaa
     1321 gacaatttac gagggctgcc aactggccgg ggcccgaggc gcgacggtca agaggttcct
     1381 cttcgaggac gcggaagatc tggagcgact cctcaaggag aatggcccat cgcctcgtct
     1441 gatctgcgtc gacggcgtga acagcatgac cggcaatgcc gtcgacctgc ctgcattcgc
     1501 ccggctggcc cgcgaatacg acgcgctctt gtatgtcgac gacgcccacg gcttcggggt
     1561 catcggcgag cgaagcgacg acgagctcag cccgtacgga aaccgcggga acagcatcgt
     1621 tcgctacgcc ggcgagacgt acgacaacgt cgtgctcgtc tcgggattgt cgaaggccta
     1681 ctcgtcgctg gcagcgttct tgacgtgccc tccggagctg aagcgtctgc tgaagacggc
     1741 cgctccgcca tatctctatt ccggcccctc tccgatcgcg tccctcgcga ccgtgctcgt
     1801 cggcctggac gtgaacgaga aacgcggtga cgtcattcgc gccgatctct ggcgcaagac
     1861 ggcagccgtg ctccacgagc tcgaccgcct cggggtgcac acgccgaatc gatccggctt
     1921 ccccatcatc gaggtgccgc tcgcgcgcca cgaggacatc gacgccgtcg ggcggttcct
     1981 cttcgacaac gggatctacg tgacgcttgc tgcctatccg ctcgttccga ggagcgaggt
     2041 cggattccgc gtccaggtca cggccgcgaa caccgacgcc gagatcgagc agttgatcga
     2101 cgtggtcggc cggctggtga acgtcttcga cctgcagccg gcacgcacga gcgagcgcgc
     2161 ggcatgaaac ggtttgccct acctggaacc ccgcgcgacc aggtctggat ctggtacctg
     2221 gccgggaccg gcgttctcac gagcctgtac ctgttcgccc cggcgcttgc cggtaacggc
     2281 ccgctgatca acgcgctcgg cctttccggt gtcgtcgcga tcgtcgtcgg gatccgaatg
     2341 cacaaacctc gcgctcgtgc tgcgtggtgg ctcttcgcgg caggacagtt cctcttcttc
     2401 agcggcgacc tctacacgta cagctatccg aagctgctgg gagcggacgt cggcttcccg
     2461 tcgatcggcg atgcgctcta tctcctggtc tatccggttc tgatggcggg cctgttcatc
     2521 ctcatcaagc gacgcaaccc acggcccgat cgaaccgcgc tcatcgacgc actgatcctc
     2581 acgatcggtg tcggtctcct gtcgtgggtg ttcctgatcg ccccgaacat ccacctcacc
     2641 ggcctgaccg tgctcgccaa cgcggtgtcg gtcgcgtacc cactcggcga cgttctgctg
     2701 cttgcggccg cgatcaggct cgcagtcgat gccggcaagc gcacgccggc gttctggttc
     2761 ctcgtcggca gcatcgtgtg cctgatggcc acggattcgg cctacaacta cgcgctcctg
     2821 aaagacacct acaaccacca gctgatcttc gacgccggtt ggattctcta ttacctgctc
     2881 tggggcgccg cggcgcttca cccctcgatg cgctctctcg aggagggagc accagagcta
     2941 ggaacgcgtc tgactccgtt gcggctcacg ctgctcggca gcgcctgttt gattgcgccg
     3001 ggaattcggt tcgtccagtc gattggcaac cccgacgtgc tcgttctcat catcgcctcc
     3061 gctgtcctct tcctgctggt cgtcgcgaga atggccggcc tcgtgcgcca ggaagcgcgc
     3121 gtggtctcgc gcgaacgcgc tctacgcgga gcgggcgtcg agctcgtcgc tgcagccggt
     3181 cacgagcagg tcgcaaacgc cgccatctcg gcagtccatc gcctgctcgc cgccgagccg
     3241 ccggtgcgac tcgtcctcgt gtcggaaggc gtagctgtcg tcgaggcctc gtccgacggc
     3301 gcgacgggcg gccgagtcgg tgaaggaacg tgtgactggc tcctgcagga gcgctcgggc
     3361 tccctgcgcg tcctgcacgc tgaaccaccc ccttacgtcc gaggggatct gaggctcccc
     3421 gaagggcaga cgacgatcgt ggctcctctg acggttcgcg gcgaagtccg cggtgccatc
     3481 gtcatctcgt cgccggtgag tctcacgcgc gacttgatag acgcgcttgg cgccctggca
     3541 acgcaggtct cgctcgccgt cgaaggcgcg tcgctggcag cagatctcca tcgtcgccag
     3601 agtgaggctc gtttccgctc cctcgttgcg cactcgagcg acctgatcac ggtgttggac
     3661 gccgacggcc tcatcacgta ccagagcccg tcgatcgagc gtttgctcgg ataccgcgtg
     3721 gacgaggtcg aaggaacgcg gttcgaccgg ctgctgagcg agaccgaccg gcccctgctc
     3781 gcgcaattga tcgcgatcga cggttcgggc gacgccgagg cgcacaccat cgagtgctcg
     3841 gtcagtcacc gagacggtgc gacgctgagg ttcgaggtcc agcacactga cctgctccat
     3901 gacgagcacg ttcgcggcat cgtgctcaac tgccgagacg tcagcgagcg taaggcgttc
     3961 gaggagcagc tcgcccatca agctttccac gatccggtga ccaagctcgc caaccgcgcg
     4021 ctgttctccg accgcgtcga gcacgcgctg atgcgcaccc agcgcggcgt gcacgagatc
     4081 gccgtcatgt tcatcgacct ggacgacttc aagacagtga acgacagtct cggtcacgcg
     4141 gcgggagacg aggtcctgca agaggtcggc cgccggctga agatcgccgt tcgtcctggc
     4201 gacaccgtcg cacgcttcgg aggagacgag ttcgccgttc tcctcgacgg tgtgtccggc
     4261 tcgtcggacg ccgccgacgc agcggcccgc atcctgcgag cactcgacct tcccatcgag
     4321 atcgaaggca agaacgtgtt tccgcgtgcg agcgtcggga tctgcctggt cggtgaggac
     4381 ctcgaggcgc ccgaggcatc ggagcttctg cgcaatgccg acgtagcgat gtacatggcc
     4441 aagcgcgaca gcaagggcag ctaccgcgtc ttcgagccga cgatgcacga gcgtgtcgtc
     4501 gaacgactcg agctgcgatc ggacctgcag cacgcgctga ccatggatca gctcgagttg
     4561 cactaccaac cggtcgtgag gctcgtcgac cgggagattc tcggcgtcga ggcgctggtg
     4621 cgttggaacc atcccacgcg cggaacgatc cccccgatcc agttcatccc ggtggccgag
     4681 gaaacgggct tgatcatccc gatgggacga tggatcctgg aaacggcctg tcgcgaaggc
     4741 gctcgtctcc gggagacctt cccgcggacc gaagggttga cgatgagcgt gaacctctcc
     4801 gtcaggcagc tgcagtccga gacgctcgtt gccgacgtgc gccaggcgct cgaggtcaca
     4861 gggctgccgg cggccgcact ggttctcgag atcaccgagt cgctgatgct cacagacacc
     4921 gacttcgcga tgatgcagct gcacgagctc aaggcgctcg ggattcggct ggcgatggac
     4981 gacttcggaa cgggctactc gtcgctcagc tatctcagcc gattccccgt cgacattctg
     5041 aagatggacc gatcgttcgt cggggcggag gacaacagca ccttgacgtc ggcgatcatc
     5101 gcgctcggca cgagcctctc gctcgaagtc gtcgcggaag gaatcgagct tcccgagcag
     5161 gccagctcgc tcgaagagct cggctgtgag ctcggccagg gatttctctt cgcccgtccg
     5221 atggattcgg atgcgttgac gaagttcctc acggttgccg gcgcgtcgac tcaggaagac
     5281 gtcgagtcgt cagcagcctc caatgcagca tagtcacgaa gcgctcgacc ggccgggagg
     5341 gtttggtcgc ggcaacctgc tctcgccgtt gaagcaccgc gacttccgga tcctctggac
     5401 cgggatgacg atttcgctga tcggcgacgg catcttcctg atcgcgatcg cgtgggagag
     5461 ctactccctc tggaacgcgc ccgctgcatt gtcgatcgtc ggcatcggca tgacgattcc
     5521 gacgatcgcg ttcctgctga tcggcggcgt cgtcagcgac cggcgcgacc ggcgggtcgt
     5581 catggcctgg gcagacggtc tccgcgcggt cgccgtcgcc gtcctggcgg ctctcgtcct
     5641 gatgggcgcc ctccgcttct gggagctcgt cgccctcgtt gcggtctacg gcatcggtac
     5701 cgccttcttc atgcccgcct tcgaggcgat cttgccggag ctgctgccca agtcggagct
     5761 gccggcggcc aacgcgctcg accaattcgt tcggcccatc gccatgaggc tcataggtcc
     5821 cgtcgctggt ggagcgttgg tggccgcatc ggcaggggtc gcgttcgcga tcgacgccgc
     5881 ctcgttcgcg gtctgcctca tcgccgtgct ggcgatgccg cggcgtgcgg gtcggcacgt
     5941 cgaagtccag acgtccagcg tcgcagcgct caaggagggt cttcgcttcg ttcgccagcg
     6001 cgtgtggctg tggggcacgc tcctgtccgc ggcgatcgcc tatctcgtat ttcttggccc
     6061 agcagaggtg ctcctcccat tcctggtcaa gaacgagctg cacgcgtctg ccggcaccct
     6121 ggggcttgtg ttggcggccg gcggtctcgg agccgtcggc ggtgcggcgt tcatgggaca
     6181 catcgggcat ccgcgcaggg acgtgacggt catgtacatg acctggacgt tcgcgacgct
     6241 ggcgatcgcg gggtacggaa tcgccaatgc ggcgtgggag ttgatgctcg tctgcctcgt
     6301 cttcaacgcg ctcgaggcgg caggcaccat cgtctgggcg acgatcaagc agcatcacgt
     6361 tcccggatcg atgctggggc gcgtctcgag cctcgactgg ctgatctcga tcggtctctt
     6421 gccactctcg ttcgcgctga cggcgcctgt ggccgcggtc ttcggtgccc gcgcgaccct
     6481 ggtcggtgcc gcggcactcg gtgccgccgt gaccctcagt gcattcttcc tgccgggaat
     6541 gcgggcgatc gagcggaccg gcggccgccg ccagggcccg tggcacgttc cggtggatgc
     6601 caccgactaa cctctccgca cggcccacga acacgaacac aggttggtga ccgaccggcg
     6661 ggcgctgacg atcgcgctcg tgctcgtcgt gctgctgatg gcggcggaga tcgccgcggg
     6721 gctgatcggc cactcgctcg cgctgctcgc cgacgcaggg catctgctca ccgatgcggc
     6781 cgcgctcgcg ttcgcgctgg tcgcgtccgc gatggccgca cgacccgcgg ccggccgctg
     6841 gaccttcggc tattcgcgcc tcgagatcct cgccgctcag gccaacggga tcaccctcgg
     6901 cctgctcgcg ctctgggtga tctggtcagc gggccaccgg ctcgccgatc cacgcgacgt
     6961 gcgcggcggg ctcgtcctgg cggtcgcgct cggtggcgtc gcagtgagcc tcgccgcgag
     7021 cttcgtgctc gcgcgcgcaa gtcgggaaag cctgaacgtc cgcggcgcgt ttctccatgt
     7081 cctgaccgac gtcgcggcct tcggcgccgc agcgcttgcc ggcgggctca tactcgcgac
     7141 cggttgggac cgcttcgacc cgatcgcgag ccttgcagtc gcggcgctca tgctctggtc
     7201 gagcgtgcag cttctccgtg agtcgacgct gatctttctc gagcgggcgc ccggggacgc
     7261 ggatcccgaa gcgatcgggc gcgcgctcgt cgccgagcag gacgtcgtcg aggtgcacga
     7321 cctgcacgtc tggacggtga cgagcggttt cccggcgctc tctgcccatg tcctggtgac
     7381 gcccggtgcg gactgccacg ccgcgcgccg ccggctcgag ctgatcctgt cggagcgttt
     7441 cggcttgacg cacacgaccc ttcaggtcga gcacgccgag ccggcgagca ggacggtcga
     7501 gctcggcgaa gcggttgcgc ggcacggtcc gctcggccgt taggctctct cagtgccccg
     7561 cctggaagga aagtcggccg tcgtcaccgg cgcgtccagc ggcatcggcg cggcgaccgc
     7621 gctggccctg gccggggaag gagcacgcgt cgtgggcggc gcgcgccgcg tcgaacggat
     7681 ccaggcaccg ctgacgccgc tcgggctgga cgtcacggat ccggagagct gcgaacggtt
     7741 tgtgaaggcc gccaccgaag agctggccgg cgtcgacatc ctgatcaacg ccgcggggct
     7801 cgcgctcggg cgggatctct tcgacgagag cacggaggag gacgagcgca cggttctgga
     7861 gaccaacgtg aacggcctga tccgcatgac gcgcctcttc ctaccgcaca tccgcgacgg
     7921 cggacacatc gtcaacatcg gctcgatcgc cggccgacag gcgtacgaga aggcggcggt
     7981 ctacgtggcc tcgaagttcg cggtgcgagg ctttacgtac gcgttgcgcg aagatctgct
     8041 cggccgcccg atccacatca cgaccgtcga tgccgggctc gtcgagtcgg agttctcgcg
     8101 tgtgcgcttc cgcggcgacg cggagacggc gcgcaaggtc tacgagggcg tagagccgat
     8161 gacgccgcag gacgtcgcgg actgcatcct gttcgcggtg acccggccag cccacatgaa
     8221 tgtggacgag atcgtcatca aggcccttgc gcagtcctcc ggcgggcgca tagttcgtac
     8281 cgagacctga tgcccggaca gctgcgaatc ctcgaaggcg ccaccttctg catctgcgat
     8341 gaggtcggtg acctccacca ccagggcgaa ggcctcttct ccgaagacac gcgctacctc
     8401 tcgcgcttca agctgaccat caacggcaac cgcccgctgc tgctctcctc ggacaagatc
     8461 gagtacttct ctgccgcgtt cttcatgcgc aacccgctcg cgggcggtct ggaccaggac
     8521 gtgctctcga tccgtcggga gcgctttgta ggcgagggga tgcaggactc cttctccgtc
     8581 cagaaccagg gcatgaagcc ggtcgaattc gatctcgagc tcgaggtcgc cactgacttc
     8641 gccgacatct tcgccgtcaa ggactacgat ttcgcgctcg gcgacccgct gcgcgccaag
     8701 ccgctgcccg agctgaggcc caacgagtac gacgaggagc gcaaccagtt cctgatctcg
     8761 gacaacggtg acgcgcccgt caagacccag gtgatcttct cccagctcgg cgaggtggac
     8821 ggttccacgg tgaagtaccg gctcgccctg caaccgcggg aacgatggaa cctggacgtg
     8881 gaagtgatac cgctgccgga cggcgagctc gtcgccccgc ggttcgcgca ccgccgtttc
     8941 ggtgaagagg tcggtcgggt ccgggactcc ctcgccgcgt ggcagctgcg agtgccccaa
     9001 ctgaggacga gctgggacgc gctcggccat tcgttcggcc agtcggtctc ggacctggcg
     9061 tccctgagga tgcagtcgga cggtgccggg atcgggaagc ttcccggagc aggcgtgccg
     9121 tggttcatga ccgtcttcgg ccgcgacacg atcatcacgt gcctgcagac actcgtcttc
     9181 ggcccggagc tcgcccgcac tgcgctcgga gtgctcgccg acctccaggc gaaggaggac
     9241 gaccccacga tcgacgcgga gcccggcaag atcgtgcacg aggtgcggca cggcaaggcc
     9301 gcccgcgcgt ggcatgagcg ctattacggg accgccgacg cgacaccgct ctatctcatc
     9361 ctgctctccg aggtgtggcg ctggacggac gatgcagcac tggtccggga tctcaaggag
     9421 ccggcgatgc tggcgctcga atggatcgac aagtacggcg accgtgatgg ggacggcttc
     9481 gtcgagtacg agcgtcgcac cgagcgtggc ctcgagaacc agtcgtggaa ggactcctgg
     9541 gattcgcagc gcttccacga cggcagcctg gcgaagacgc cgatagcgcc gtgcgaggtg
     9601 cagggctacg tctacgacgc gaagcagcgc attgccgagc tcgcgcgcga ggtatggcgc
     9661 gagcgggagc tcgcgacacg gctggaacaa gaggcggaag agctgcgcag gcgcttcgac
     9721 gatgccttct gggtggaaga gcgcggcggg tattacgcac tcgcgctcga ccgcgacaag
     9781 cgcaaggtcg actccctctg ctccaacatc ggccacctgt tgtggagcgg aatcgttccg
     9841 cctcaccgcg tcgacgcggt cgtcgaccag ttgatgggag acgagctctg gtcgggctgg
     9901 ggcgtgagga cgatgtcgac gggcgacgcc ggctacaacc cgctctcgta ccacaacggc
     9961 accgtttggc cgcacgacaa ctcgctgatc gcagccggac tggcgcgtta cgcgcgctgg
    10021 ccggaggctc agcgcatcac gcagcgcatg ctcaccgccg cacaacactt caactaccag
    10081 ctgccggagg tcttcgccgg catgcgccgg tccgagacac cgtttccgat cgcatacccg
    10141 acggcagcgc gaccgcaggc gtgggctgcg gggacgccgg tgctgctcct ccagctgctg
    10201 cttggcctcc agccggatcg ccggcgccac gccctcgaga ccgttgcgcc gctggagctg
    10261 ccgtcgtggg ccggaacgat caggctctca ggcatccgcg cgttcgaccg gctgtgggac
    10321 gcgcgcatcg acgacggcaa agtccgcgtc gaagaggcat gatcgacctc ccatgaacat
    10381 cgcgatcctc gcgccggtct ggttcgcggt tccgccgatc ggctacgggg gcatcgagtg
    10441 gatcgtgtgg ctgctcgccg acgggctcgt cgatgcgggg cacgacgtca cgctcttcgc
    10501 gtcaggcgac tcgcgcacga aggcgaagct cgccgcagtc ttcccggaag cgccgagccg
    10561 gcagatcggc cgcacgtact gggagctgaa ccatgcgctg tcctgcttcg gccgagcgga
    10621 cgagttcgac gtcatcaacg accacacggg catgctcggc gcaaccctcg gcgcgacgac
    10681 atcgacgccc gtcgtccaca ccgtgcacgg gccgctcgac ggcgagcccg gcctgatcta
    10741 cgagaagatc tcgcgggtcg cgccgcaggt cggcttgatc tcgatctcga tgaaccagcg
    10801 cgagccgaag ccggacctga actggatcgc caactgcccg aacgcgctcg acttttcggt
    10861 ctaccccgtc aagccacacc gcggtgagta cctgctcttc ctcggccgga tgagccccga
    10921 caagggagcg catcgcgcgg tcgcggtcgc aatggagacc ggtctcccgc tcaagctcgc
    10981 cggcaagatg caggagccga aggagcgcga gtacttccac gagctcgtcg agccgcacct
    11041 cgtcgacgga atcgagtacc tcggcgaggt cacgcacggc gagaaggtgg agctgctgca
    11101 gaacgcgcgc gcgacgttgt tcccgatcga atgggaggag ccgttcggcc tcgtcatgat
    11161 cgagtcgatg gcctgcggaa ctccagtgat cgcgacgcgc tggggtgcgg tcccggaggt
    11221 gatcgaggac ggtcgcagcg gcatcatcgt cgatcactac aagcagatgg caggcgcgct
    11281 cccggctgcg gacgacctcg atccattcga gctgcgacgc tacgtcgagg aggagttctc
    11341 gccggagcgg atggtgcgcg actacgtcgc tgcttacgaa aaggcgattc aggggcacta
    11401 accccagggt cctgcgtccg accagccgtg ccagcgctcg ggctcgagcc aagcgctaac
    11461 tcgcttggcc tctcgccgac gaaacggctg ccccgtgtag cgaacggcga gtcgatcgat
    11521 gtcgacgagc tcgggatcgt cctcgatcga gacgactctg ccgatcaggc tgatgtggct
    11581 gtaccagctg cgtccgtcga gcacggtcag cgcagcacgg ggatcgcgac gcaagaaccg
    11641 caggcgcagc ctgctttcgt ccatgttcag cagtacgcgg ccgctctccc agtcgtacca
    11701 cgtgggcacc gtgtgcggtg agccgtccgt tcggagtgtc gcaacgaccg cgggattcgc
    11761 gcgcaagagg aactcgtcga cgtcgggggg aacggggacg gtcggcacgg cggtaagcct
    11821 acgaggcggc gaccgcgggc gcgagctcgg cttcggacag cccgacgggc tcgagacgga
    11881 agttccggac gtcgcgcacc gagatcacga tcaaggttgt cacgacgatc cagacgacac
    11941 cgatccaaag ggacgtcgac atgccgatga cgtcggcaac gggtcccgcg atcgcgtacc
    12001 cggcgggcag gaaggccatg gcgcccatcc agttgtacgc gctgacgcgc gccagcttct
    12061 cgggcgcgat cgtctgctgc agggccgtgt cccagatcac ggttccgtac gagaaggcga
    12121 agccggcgag aaccgcggcg gcaccgatgg cgagggccgg cgcgtggatc gccagcagtg
    12181 cgcactgcag tgccgtcgtg atgaagaggg cgccgatcga ggtcatggca cgacgcggtc
    12241 gcaggcggag cccgaggagg ccacccgcca gcgccccgat cgcctcgccg gtgacgatga
    12301 tcgcccaggc cgtggcaccg cccagggagt tcttcgcgat gtaaggccca agcacgaaga
    12361 acggtgcgta ggtcacgagg aagtacagag agatccagcc cgtgagcagc cagacccagg
    12421 tgtgctcggt aaacgcctgc cacccttcgc gcagttcgcg caggaagttc ggagaagccg
    12481 ccgtccgcgc ccggtctggc agacggatcc ggctcaggag gagcgcgctc gtcgcatacg
    12541 tcgccgcgtc tacgagaagt gcgtaaccgc tgccgacggt cgccacgatc gtgcctccca
    12601 ccgccgcgcc gaccggaaag gccaggtacc gggagatgct catgaacgcg ttcgcctgct
    12661 gaaggagggc cggtcgaacg gtctcgggca cgagacctgt cgaggcgggc gaatagaacg
    12721 cggtcgctgc gccgccgagc gcctgcaaga cgaagatctc ccagatgcgg gcgttcccgc
    12781 tgacgagcag ggcgcccatg gcggcgcgaa tcgcgaagtt cgccgagtcg gacgacagca
    12841 tcacgagacg ccgggggaga cgatcggcga cgacgccgcc gatcaacaga gtggagacct
    12901 gggccagtgt ccagcaggcg aaggcgattc cgacgtcggt cgcggacccc gtcagatcga
    12961 ggacggcgaa ggcgaccgcg atcggcgcga gattcgtgcc gaagaacgag atcgtgcgcg
    13021 cgccggcgag caggcggaag ttgcgcacgc gcagagggcc gagctcaacg agcacggcgc
    13081 tgttcccagt agcgtttcag gcctgcgtag gactgccaga acggcattgc gcgctcatac
    13141 gtttctacgt cgccgccggt tgcagtcacg tcactgcgag ccgactcgct gaactcctct
    13201 tctgttacgc cctgttcgac tcgacgcgtc cagtctccca gccgttgccg gagctccgca
    13261 aagtgcgact caggatcggc ggcgacgccg aagtggacga gtgccagcct gctcggcttc
    13321 cgcctctcga tctcgtcgat cgtccgttcc cacgctgcga ggtcgaagtc cggcggcggc
    13381 gtcggcggta ggaccgttcg atcgggctgg aggcggactc cggccgcgtc acctgcataa
    13441 agggtgccgt cgccggcgag gtaacagacg tggtgacttg cgtgcccggg cgacggaaag
    13501 cagtcgaggc cgagcacgtc cggtccgaca acgtggatgt tctccgtcgg gaccggggcc
    13561 agctcccccc agagcgcgtc gaacgtatcg ccgtagagac ggcgcgcgct cgcttcgagt
    13621 cggctcggat cgatgagatg gggcgcgcct atctccgaga catggacctg tagctcagga
    13681 tgctcgcgaa cgaggactcc ggcggcgcct gcgtggtcga ggtggatgtg cgagaggagc
    13741 aggtggcgaa cgtcctgaag ctcgagcccg cgttggcgca ggcccgagtg caaccggtcg
    13801 atgcaggacg aagggccgca gtcgtagagc gccgggccgt cctttgtctc gaggacgtag
    13861 gagccgatga cgcgctgggc gccctggaag tggaggtcga gaggatccgg gctcaccgag
    13921 cccggatcct cccatgcgtc agaccgacgt ggagcgcccg cgcttccgac ggaagagggc
    13981 cagagctcct gcggcgaggg cgacgcccgc gccggcaccg acggcgacgc cgagcttgcg
    14041 ctctgcgcgg gttttcctcg cgggcgccct cggcggcagg ccggccttta cgagcgtccc
    14101 tctcagcgcc gccgtgccct gataccagcc gcccggcgtc gattgggtgt cccagaacct
    14161 ctgagcgggc gccatgtagg tgaccacgcc cttgaccgcg tagggataca gatcctggtg
    14221 aagcgtggag tcgccgttcg gtccgcccgg cacggtgtac gtcacctcgt agcgcgggcc
    14281 gagcccgcct aggggacgcg atgtcgtcgc ggacggtgtt gcaccgaatg cttgcgagaa
    14341 gaagccgccg tcgctgacga ggatcccgag ggcggtgtcg tttccgccct ccccgtaccc
    14401 ctcgatgttg accgcatgtc tcaggccggg gccggtgaca ctcgccgccg acggtccctt
    14461 cgcagtcgcc gcggccggaa acaacagcac ggcggtgagc gccgccgcga gcagaagtgg
    14521 gcgtctcatg ggtcgctcct tggtcgtttg aggtgctaca ccgaccgggc ggagtgggtt
    14581 ccgctgacaa ccttggcgaa cgccgcctcg agatcctcga ggaggtcgtc tgcggactcc
    14641 aggcccaccg agagccgaat caggttccgc ggcgccgcga acggcgcatc cgcagtcgac
    14701 gcatgcgtca tgcgcgcggg caattcgatg aggctttcga cgccaccgag cgactcggcg
    14761 agcttgaaga tcttcgtgct cgcgacgaag gcagtggcct cttcctccga ctcgaccagg
    14821 aacgagatca tgccgccgaa gtcgtgcatc tgcttcgcgg cgatcgcgtg gcccggatgc
    14881 cttgggagcc ccggatagag ggtctgctcg acgcgcggat gccgttcgag aaagccggag
    14941 agcgcatgag cgttccggca gtgctggcgc atgcgtacag cgagcgtttt gattccccgc
    15001 agcacgagcc agcagtccaa cgggctcggg acggccccaa gggacttctg gaggaacaac
    15061 agccgttccg cgatcgtcgg atcgttcgtc gcggcgaagc cgccgacgac gtccgagtgt
    15121 ccgccgaggt acttggtcgt cgagtgtacg acgacgtccg ccccgagctc gagcggccgc
    15181 tgtagatacg gcgtcgcaaa ggtgttgtcc accacgagga gcgcgccggc cgcatgcgcg
    15241 gcttcggccg ccttctcgat gtccacgatg ttcagcagcg ggttcgacgg ggtctcgatc
    15301 cacacgatcc gagtgcgctc gtcgaggtgc gaggcgaggt cgtcgaactc ggccgccggc
    15361 agatagtcga agacgtagcc cttgggctcg tagacctgcg aggtcatgcg gtagacgccg
    15421 ccgtagacgt cggcgatcag caccatccgg tcgccggggc tcagcaggtg catgatcgtg
    15481 ctgaccgcgc ccaggcccga ggcgaacgcg actccgtgat ctgccgactc gagtgacgcg
    15541 aggcactgct gcagcgcggt gcgcgtcgga tttgcaacgc gcgagtagtc gtaccccttg
    15601 tgttctccga cggcgtcctg cacatacgtc gacgtgaggt agatcggcgt cgtgagcgcg
    15661 ccggtcgctg gatccggctc ttggccttcg tggatggctc gagtttcgaa gtccatctag
    15721 gtctcgatgc tacccgcacc gatgaaggtc gcgaggctgc gcaacggctt ctgcacagac
    15781 accggcttca cgcccggctc ccaccaaatg ccgttgacgt gtagaacccc cgtcttgcgg
    15841 tcgagcacgg gatcgatcct gccgatcagc cgatcgcctc gcaggatcgg gaggacgtag
    15901 tagccgtatt cgcgctttgg tttcggcacg tacatctcga gccggtagcg gaagccccag
    15961 agtgcctcag cgcgatcacg gtcgtggacg aggcggtcga agggcgacag gaacgtcacg
    16021 cgtttcggca tcgggccgtc ctcggcgtcg ggatgcgctt gccattcgcc gttctcgaac
    16081 ctcacgccaa gggcacggaa gctcttctcc gcgagcaact gcttggcttc gggccaaggg
    16141 atgctctcgg cctcggggta ccaccgctcg gcgaggtccc acagacgctg cttgccccgg
    16201 cgtccgacga tcgcaacgac accccgacct tccaacagtt cgagcatgag cgtcacctt
//
LOCUS       DASVEU010000202        17284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_530342,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000202 DASVEU010000000
VERSION     DASVEU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530342"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF225_08380"
     CDS             <1..240
                     /locus_tag="VF225_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145312.1"
                     /translation="VIGLRQKLGLAESTLWVPVFGTANEVVWEIEYPDLATFQRENDA
                     FYSDADAMKQWRRLWQHAVQGSIQDELLEEAPNIA"
     gene            complement(284..727)
                     /locus_tag="VF225_08385"
     CDS             complement(284..727)
                     /locus_tag="VF225_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145313.1"
                     /translation="MVIACLALAVALSGTSYAQILNVPVNSVGTAQLKANAVISSKVK
                     DHSLLAADFKPGQLPGVSGYEVVEGGPSIVMNQVYNSVTMTCPAGKKAIGGGGGTGGG
                     IVPGDGPYIVVNMPSSDGSGWLVQTARATSGYSALVGRVICAKVS"
     gene            1043..1306
                     /locus_tag="VF225_08390"
     CDS             1043..1306
                     /locus_tag="VF225_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145314.1"
                     /translation="MSEVYLKLTGKWANIDGKLLKVPPMQPENSDAQTGIPGGHPAVS
                     SWYEEGELGICPECGQQTLLPPNEMAEPATICLTCGALDVLPK"
     gene            1708..2103
                     /locus_tag="VF225_08395"
     CDS             1708..2103
                     /locus_tag="VF225_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7145315.1"
                     /translation="MTRFVVDASAVLHLASAEVEVSAAHKLLAPTLLRSQTLSALHEA
                     VEQGEIPADVARERLTRIRGMKIRLLGDAVLQRRAWDLADQLGWASTYNAEYVALTQL
                     QADALVTLDTELARSVEGIVATAPIEALR"
     gene            2209..2757
                     /locus_tag="VF225_08400"
     CDS             2209..2757
                     /locus_tag="VF225_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145316.1"
                     /translation="MFLFASALILAAGVSGATARQQQGCATPVYENGLDLVFGRGSRA
                     AADKIAQQAVTVGFQGVKTVQESCTRWKAVLRGLDSFSTAVGVQAEARTVHRFPTIEC
                     VTSEQTGQLQAIFGTLPTISELNVVIARANSFGYVGLKIKRAPCGGYQAYVAGFSDKA
                     QALEFAQTARERSGLRVTIIKA"
     gene            complement(2788..2859)
                     /locus_tag="VF225_08405"
     tRNA            complement(2788..2859)
                     /locus_tag="VF225_08405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2825..2827),aa:Val,seq:cac)
     gene            complement(3196..3290)
                     /gene="ffs"
                     /locus_tag="VF225_08410"
     ncRNA           complement(3196..3290)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF225_08410"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3390..4238
                     /locus_tag="VF225_08415"
     CDS             3390..4238
                     /locus_tag="VF225_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7145317.1"
                     /translation="MTDARGRGVGPGGIAVGGGGLGLVGLAIYLLIALLSNGGSLGQL
                     EPLDNQQVGRGDTPSSIDTACRTGQDANQRQDCRIVAVVNSVQKFWDGVFQRSNRQYQ
                     YVDTVFFSGQVDTACGTADSQVGPFYCPRDKLVYIDLGFFDELESRFGVGNAVFVQAY
                     VLAHEYGHHVQDQLGVLDKIAGDRQGPESLSVRSELQADCYAGVWAAHAVETGIVEEL
                     TQADINDGLDATAAIGDDRIQQQTQGQVNPETWTHGSSEQRRRWFSQGYEQGRPAVCD
                     TFSGSI"
     gene            4261..5847
                     /gene="dnaX"
                     /locus_tag="VF225_08420"
     CDS             4261..5847
                     /gene="dnaX"
                     /locus_tag="VF225_08420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7145318.1"
                     /translation="MAALYRKYRPQDFDEVVGQEAIVRTLKNAITSGQLRQAYLFAGP
                     RGTGKTSLARILAKGLNCVQGPTATPDKVCNPCVTIASGTSLDVIEMDAASQRGIDDI
                     REIRERVVLQPAEGRYKVYILDEAHQLTDAAWNALLKLIEEPPPHLVFVFCTTDLSKV
                     LPTVRSRCQTFVFARPRLPELVRLLRRVADGEGIQVPDQALALVARGARGSFRDAVST
                     LDQLASATENEITVQSVLQLLGAVEEEALFRLCDLVVDRDTAGALTFVEELSEQGQDL
                     GRLVVDLLEHLRHLMLVQHMGEVPDTLPVTEETRERLRAQANQLGEATVIRLVDLLAV
                     AIEDMRQGGDPRLPLELALVKVTRPGADLSRESLSYRLEQLETRGHVPTAPASGTVPE
                     TSPEQAPVEAVALAAPPSLELEQLQEAWQRTILPAVEERSIPAASVLREAHPAELAND
                     TLTVEFPSSAAFHRQLAEEPKNATLLADALYEVTGRRLGLAFAEGEARADAVEDDEPA
                     GEEKILELVKETLDAHERED"
     gene            5862..6179
                     /locus_tag="VF225_08425"
     CDS             5862..6179
                     /locus_tag="VF225_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7145319.1"
                     /translation="MNQLFQQVGQMQEQMQKAQAELANETVEATAGGGMVTVVANGAG
                     EIRQIKIDPKAIDPSDPEMLEDMILAAVNEAIRAAQNLMESKLGGLAGGALGGLGLPG
                     LGE"
     gene            6179..6550
                     /locus_tag="VF225_08430"
     CDS             6179..6550
                     /locus_tag="VF225_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7145320.1"
                     /translation="MRDRLGALPLFADLDDAQLDRLAAATTEFEAPAGQALIERGRAG
                     SGMFVLEEGAAVVEAPEGTRELGPGDVFGERALLGEDIERTARVRARTDVRCLAIARP
                     EIERLLAEDPRVAARLGQLST"
     gene            6599..7192
                     /gene="recR"
                     /locus_tag="VF225_08435"
     CDS             6599..7192
                     /gene="recR"
                     /locus_tag="VF225_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7145321.1"
                     /translation="MLSPAVENLVAQLSRLPGVGSRTAHRLAFHLLQRPTDEALALAA
                     AIEEVKERVRFCDECGNLTEEETCAICRDTRRDHTLICVVEQPVDLISVEKTSEFRGL
                     YHVLGGALSPLDGVEPEHLRIDELLGRVERNGVLEVVLATNPNMTGEATAAYLADRLR
                     GRVRVTRLASGLPVGGDLEYADEVTLGRALAGRREME"
     gene            7189..8481
                     /locus_tag="VF225_08440"
     CDS             7189..8481
                     /locus_tag="VF225_08440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7145322.1"
                     /translation="MTTISYEAVPAVEGDAAAAPGNTLVMKFGGTSVGDTQKIRDVAR
                     RLVTAREAGQRVVGVLSAMGDTTDNLIRLAHEMSPKPHPREYDMLVSVGERISNALCA
                     MAIRDLGHEAVSLTGSQAGIVTDTFHGKAKIVEVRARRLHAELDRDQIVLVAGFQGVS
                     TELEVTTLGRGGSDATAVALASALGGACEIYTDVEGVFTADPRVVADALKLPKISYEE
                     MLEMAATGARVMMARSVEIARRYGVPLHVRSAFGDSEGTWITKEENLVLEKAIISGVT
                     HDTSEAKATVLAVPDRPGIAARIFRALADAGVNIDMIVQNISAEGATDISFTLPKTDL
                     PAAEPILDVLASEIAAKGVVYDPDVAKVSLIGAGMKSHPGVAADMFEALGEAGINIEI
                     ISTSSIRVSCVVRATEVERAVQAVHDRFRGYAEERVGA"
     gene            8474..9412
                     /locus_tag="VF225_08445"
     CDS             8474..9412
                     /locus_tag="VF225_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX7145323.1"
                     /translation="MPEPRIAVVGATGAVGAVTLELLRERGYGQVRALASARSAGKEL
                     AGGGVVEEATPEVLAAGDIDLAFFSVGTSASRELVPHAVRGGAVAVDKSAAYRLQEGV
                     PLVVPEVNGERAREHDGIVANPNCCAIPLTMALKPLHDAAGLARVRVSTYQSVSGAGA
                     QRMEELRATPSGDGNLAMDWDFDGEEFDEESKLRDETRKILELPELPISATCVRVPVL
                     VGHAEAVWVETEGPLSADDARGILGAAPGIKLEGFPTPRDATGIDEILVGRIRRDPTV
                     DNGLVFFVAADNLRKGAALNAIQIAELILQRRPVAA"
     gene            9466..9798
                     /locus_tag="VF225_08450"
     CDS             9466..9798
                     /locus_tag="VF225_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX7145324.1"
                     /translation="MSIFVLLLFGRRSDAREVARFLPDCIVLLKRLLRDNRVPRRAKV
                     VLGLLVLYLVMPLDLVPDFIPVAGQLDDAVLVAAAIAYVARSAGRGVIEELWPGSERA
                     LNVVLALA"
     gene            complement(10000..10626)
                     /locus_tag="VF225_08455"
     CDS             complement(10000..10626)
                     /locus_tag="VF225_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145325.1"
                     /translation="MTGPRVTGLTGVTLQDAPTVCHDCVWWQSREGRTTDKRKWIEKA
                     ESEWGPWGTVYYDGDGHVLGSMQYGPAGLFPRAAELPAGPPSDDAVLVTCAYLVETSS
                     PWVMQSLFLAAIGDARDRGAKALETFAYRYREGESQYERFLVHKTIFPSDFLADFGFQ
                     TLRRFGHVELARLEFGGLLPVLEGKREKVMRAVKEAFTPAPAPVPPGP"
     gene            complement(10623..10913)
                     /locus_tag="VF225_08460"
     CDS             complement(10623..10913)
                     /locus_tag="VF225_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX7145326.1"
                     /translation="MATISSDILASYAADAAREVEGVRGLVESSLHRRKGVRIVDEDG
                     RVRVELHLAVEWGVSIPEVGREVQHRVAAYLERMAKVDPEAVDVIVDEIGPP"
     gene            complement(10913..11653)
                     /locus_tag="VF225_08465"
     CDS             complement(10913..11653)
                     /locus_tag="VF225_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7145327.1"
                     /translation="MRLRTLDVPLARLSEELAGLRIAHLSDFHFGLPSRGTVAVERAI
                     DWVEQRKPDLVAITGDLVSRPRGEALLREQLGRLERPFVVLGNHDVEHSRDPFSRAAG
                     LTDLTPARLLVDESETVELRGHRVQLVGVDPRAYRQGRSRPWELIDESADLRILLCHF
                     PYVLGFLPPGAFDLVLSGHMHDGQIALPLGRNRKFRFAHPSVRFATGLYELPGGTMHV
                     SPGLGTTFVPFRFFARPEATELVLKRPP"
     gene            complement(11725..12609)
                     /locus_tag="VF225_08470"
     CDS             complement(11725..12609)
                     /locus_tag="VF225_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX7145328.1"
                     /translation="MRRVAYYKGCLASLSAKELDSSTQALAPKVGLELVELETVTCCG
                     AGDIHEAEPDYYLHLNARILAYAEAAGCDTLLTVCNVCTLNLRQANWQLKNDENLLDR
                     VSTNLERVGVPPYSGNVEVRHLLWEIAEGEGYELLKQSAHKGLKGLKIAPFYGCQILR
                     PSKILGFEDPDRPWSLERIIEACGGEAIDYPAKIKCCGFPIIQAREETALGELIQPIE
                     QAKEAGADVIVTPCPLCHLSLDAWQSKLKKATGKDFAMPILHLSQLVGVAAGLEESEL
                     KFKRHVVSVTPVLEKLEV"
     gene            complement(12606..13688)
                     /locus_tag="VF225_08475"
     CDS             complement(12606..13688)
                     /locus_tag="VF225_08475"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX7145329.1"
                     /translation="MHSELAPAPGSTTIDVALKIWRYDAETGEKELKRYEVEAPDWVT
                     LLDVLDIIKDKHDGTLAYRKSCRMMICGSCGMRMDGAAVLACKTRMYDIAKSGHVPVI
                     SSMGNMPVIKDLVVDMDPFWGKIRRMKPWLDPGYEEPLEKERIVSQQQMNVIHKEALC
                     IMCGCCVSECNSMESDPDFFGPAALAKGMRFVGDVRDQADVARLNDYNDEHGIWDCTR
                     CYFCNERCPKGVDPRDAIAKLGAESIKEGIDHDMGAKHAQWFVTSAKTTGWLRETELV
                     PKTQGVVEAIKQTRFALSLVKHGKVPPPFPPHVADRVGEARKLYHLVKEQGRDGALGI
                     VQGEHALGRIEFSEQAGVKTTETETT"
     gene            complement(13688..15370)
                     /locus_tag="VF225_08480"
     CDS             complement(13688..15370)
                     /locus_tag="VF225_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7145330.1"
                     /translation="MEAKHDVLIVGAGCAGMRAAIEAHDAGADVALLSKIHPVRSHSG
                     AAEGGINAALGNASEDSPAEHAYDTVKGSDYLGDQDAIEILCNEAPGDVYQLEHWGAV
                     FSRTDDGRIAQRPFGAAGAPRTAYAADITGHVLVHVLYEQVMKREIPTYEEFFAWKLV
                     MNDDRCQGVIAWDLLNGGLKSIGAKTVILTTGGAGRLYVGTTNAYACTGDGMALALRV
                     GVPLKDMEMMQFHPTTLSPTGVLITEGTRGEGAYLLNSEGERFLKRYAPNAMELASRD
                     VISRAEQTEIDEGRGINGNVILDLRHLGAEKILERLHGTRELSMTFAGVDPIYEPIPV
                     RPGAHYHMGGVDTDVWGLTEVEGLYAAGECACVSVHGANRLGGNALMETITYGKRVGR
                     HAADWALSNTTVEVPPSVEADAERELKELLDRREGERPWSIRDELAESMHENFGVFRR
                     EEQMRKQGEIVTGLRERYESVVVEDKGNVFNNDLTQALELGFLLDLAACMVPCGIERK
                     ESRGAHARPYDYPDRDDEHYLKHTLVSWADGEPRVDWKPVTMTNWKPQERTY"
     gene            15636..17159
                     /locus_tag="VF225_08485"
     CDS             15636..17159
                     /locus_tag="VF225_08485"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX7145331.1"
                     /translation="MLFRLPQAKRVIENPSPEEVKELAAKMPTARPTKYGNLNVQTQV
                     LARSKRSTFLVTDEPDGQNQALSTDDAARWAARQDEYIAGREMVVIDGYIGTDLEFRT
                     STRLYVEAANANIAGMQQQLYFGPPEEDFEPELTVIYTPNLKAEGFPDDRLIMVDLEQ
                     GVTRVFNSDYFGESKKGGLRMWNKLVYDQGGLPLHAGCKIIPTDDGDKVCLTVGLSGT
                     GKTTTTFTKQNGSLPVQDDFVAWMPDGGVYVTENGCFAKTYGLNPEDEPTIYGAVTQP
                     NSYLENVSQKGAELDFYDESYTQNGRATFPFGVIESAADREIDDAHFLLILNRNENVI
                     PAVAKLEGPQAAAYFMLGETKGTAAGGADEAGKSLRVPGTNPFFPMLHDLQGNRFLEL
                     LEEHPLEVYLMNTGRVGGPEADERSKKVRIKHSSAIVKGIAEGAIEWERDPDFGYLVA
                     SAVPGIDDVEVLQPRKLYERTGRADEYRAQVERLKSERTEFLGKFGSLSAEIVAAAS"
BASE COUNT         2965 a         5766 c         5683 g         2870 t
ORIGIN      
        1 gtcattgggc ttcgacagaa actcgggttg gccgagtcga ccctgtgggt acccgtgttc
       61 ggcacggcaa acgaggtcgt atgggagatc gagtaccccg atctcgcaac cttccagcgc
      121 gagaacgatg ccttctactc cgacgccgac gcgatgaagc agtggcggcg gctttggcag
      181 catgcggtac agggctcgat ccaggacgag ctgttagagg aagcgccaaa catcgcatag
      241 cgcctgccaa gttcggtggg cgcattcgtg ggcggccgtc ggctcaggac accttggcgc
      301 agatcacgcg accgaccagg gccgagtatc ccgacgtggc gcgcgccgtc tgcaccagcc
      361 agccgctgcc gtcgctggag ggcatattga cgacgatgta cggaccgtcg ccggggacga
      421 tcccgccgcc ggtgccaccg ccccctccga tcgccttctt tccggctggg caggtcatgg
      481 ttacggagtt gtagacctgg ttcatcacga tgctcggtcc tccctccacc acttcgtagc
      541 cgctaacgcc gggaagctgc ccgggcttga agtccgctgc gagcagcgag tggtccttca
      601 ccttggacga gatgacggcg ttcgccttca gttgcgcagt gcccacgctg ttcaccggta
      661 cgttcaggat ctgcgcatag ctcgttccgc tgagggcaac cgcaagcgcg aggcaggcga
      721 tgaccatggc cgccgatggc ctagggatcg atcgaaacat gacactcctt tctgggccga
      781 tcggacgcag ccgggcccga atcgtgacca gggaatggcg acgccgtctg cggcaacgac
      841 gccgccttgc atccagaacg cgcgggcgga tcactccgcc gcgcgctcag gtcagtccct
      901 gggctccggt tggcttcaac ctcggccgca tcagtccttt gccggtcaaa aggaagaaag
      961 ggccggcgtc gattgccgca gtcgggcgcc ggcccttcct gagccatcga aatggctacc
     1021 cgcggcgatg atacccaaaa gggtgtccga agtctattta aaactgaccg gaaaatgggc
     1081 taatattgac gggaagctac tcaaagttcc gccgatgcag cctgaaaact ctgacgctca
     1141 gaccggcatt cctggtggcc atccggccgt gtcctcctgg tatgaggaag gcgaacttgg
     1201 catctgtccc gagtgcgggc agcaaactct tctgccaccc aacgaaatgg cagaaccggc
     1261 caccatctgt ctcacgtgcg gtgcgctgga tgttctgccc aagtagtcgc cgcgtgcctt
     1321 cggcgagtac gttcctcgcc tgttggtaga tagaagcgaa gcgcgacaga acagttcggc
     1381 ccccgcgttt ggaaagacca acgccgagtg ggccttctta cgtcggatga ttgctactgg
     1441 tgcgggcggt gagagatagt tggcgccaca acgccaacag tcagacggcg ctcgcaccaa
     1501 tctctacgct cggcaagcgc gatgccggcg aaaccgccgc agttcccgtt tcaccggatg
     1561 atgatcgtcc aacggcccat gggttcctct ggtggtgccg cgttgatgta caggcgatag
     1621 cgaccggcgg gatttcggta caggattgtg gtcggtaggc ccttacgcct agtcttccgg
     1681 ggtgcagatc gccaacgcgt agccgtgatg actaggttcg tcgtcgacgc cagcgccgtc
     1741 ctccacctcg cgagcgcgga ggtcgaggtc tcagctgcgc acaaactcct cgcaccgacg
     1801 ctccttcgtt cccaaacgct gtcggctctg cacgaggccg tcgagcaagg cgagattccg
     1861 gccgatgtcg cgcgcgaacg cctcacccgt atcaggggga tgaagatcag actcctgggc
     1921 gacgccgtcc tccagcgtcg ggcctgggac ctcgcggatc aattgggatg ggcctcgacg
     1981 tacaacgccg aatacgtcgc actcactcaa ctgcaggcag acgcgttggt gaccctggac
     2041 acggaactgg cgcgcagcgt cgaaggaatc gtcgcgacgg cgccgattga ggcgctgcgc
     2101 taggaaggct gggccggccg tcggcccgcg ccgttcaatt cggacgatcg ccaaccgtgc
     2161 gatctgatat ctgttccggc tatgagaaag gcgatgattc cactctcgtt gttcctcttc
     2221 gcttccgcac tgatactggc ggctggggtg tcgggtgcca cggcaagaca gcagcagggt
     2281 tgcgcgacgc ccgtgtacga gaatggactc gacctcgtct ttggtcgtgg gtcgcgggct
     2341 gctgccgaca agattgccca gcaggcggtg acggtcgggt tccagggcgt gaagaccgtg
     2401 caagagagtt gcacgcgctg gaaggctgtt ttgcgcggcc tcgattcgtt ctcgacggcg
     2461 gtgggggtgc aagccgaggc gcggactgtt caccgcttcc cgacgatcga atgcgtaacg
     2521 tcggagcaga ccggacagct tcaggcgatc ttcggaacgc tgccaacgat cagcgaactc
     2581 aacgtggtga tcgcaagggc aaacagcttc ggctacgtcg gcctgaagat caagcgcgca
     2641 ccgtgcgggg gttatcaggc ctacgtcgcg ggtttcagcg acaaggccca agccctggag
     2701 ttcgcacaga cggcgcgcga acgctccggg ctccgggtca caattatcaa ggcctagacc
     2761 cgacctacta cggccttgct gagccgatgg gcgcacctgg gatcgaacca gggacctccc
     2821 gcgtgtgaag cgggcgctct cccgctgagc tatgcgcccg agagcgaatc gagaataccc
     2881 aacgagattc gatctaacct cggcgagggc cgtctcatcg cgaacgtacc ggtgccaggc
     2941 acgggtacga aacgcatgag gaagcgtcac gcaactcagc gatccccctt gcgccgcagc
     3001 atccccgggc gcgtaccggt gccaggcacg ggtacgcaac tgtgacgagc gcgtcacgga
     3061 tccgcctagc tgagcaaatc gaggatcccc aacccatccc gccgccactg ccggcttagc
     3121 cggcggtggc cgctgcggct acgcatccat cccatcctcg aaaagtcatc atgcctcgcg
     3181 aagcgaggcg gacttttcga ggcggtaacc gcggcacgta ggaggaatgc ttagcgctgc
     3241 tgcctcccgg ccctgacgcg gttcacacac tcctactgcc gcggcgcggc gatggtagca
     3301 gcgggatcta gcggtggttt gcggagcgtg gaaccaggca aagtaccgtc gtgcgctttc
     3361 gccgaaatgc accgcttgat ccggggcagg tgacagacgc tcggggacga ggcgtcggtc
     3421 caggaggaat cgccgtcggt ggcggcggcc tcgggctcgt cgggctcgcc atctacctcc
     3481 tcatcgcgct gctctcgaac ggcgggagtc tcggtcagct agagccgctc gacaaccagc
     3541 aggtgggccg tggggacacg ccgagcagca tcgacacagc ctgccggacc ggtcaggacg
     3601 cgaaccaacg gcaggattgc cggatcgtcg cagtcgtcaa tagcgtccag aagttctggg
     3661 acggcgtctt ccaacgaagt aacaggcagt accagtacgt cgacacggtg ttcttcagcg
     3721 gccaggtcga cacggcgtgc ggcacggccg actcacaggt cggtccgttc tactgtccac
     3781 gcgacaagct cgtgtacatc gatctcggtt tcttcgacga gctcgagtca cgcttcggtg
     3841 tcggcaacgc cgtgttcgtg caggcctatg tgctcgccca cgagtacggc catcacgttc
     3901 aggaccagct cggggttctg gacaagatcg cgggcgaccg ccagggcccg gagagcctct
     3961 cggtgcgaag cgagctgcaa gccgattgct acgcgggtgt ctgggcggcc catgcagtgg
     4021 agaccggaat cgtggaggag ctgacccagg ccgacatcaa cgacgggctc gatgccactg
     4081 cggcgatcgg cgacgaccga attcagcagc agacacaggg ccaggtgaac ccggagacct
     4141 ggactcacgg ctcgtcggag cagcgccgcc ggtggttctc ccagggctac gagcagggcc
     4201 gaccggccgt ctgcgacacg ttctcggggt cgatctagcc cgagggctag tatcgccggg
     4261 atggccgcgc tgtaccggaa gtaccgcccg caggacttcg acgaggtcgt cggccaggag
     4321 gcaatcgtcc ggacgctgaa gaacgcgatc acgtctggtc aactgcgcca ggcctatctc
     4381 ttcgccggcc cgcgcggcac cggcaagacc tcgctggcgc ggatccttgc gaagggcctc
     4441 aactgcgttc agggacccac ggccacgccg gacaaggtgt gcaatccctg cgtcacgatc
     4501 gcgagcggca cctcgctcga cgtgatcgag atggacgccg cctcccagcg cgggatcgac
     4561 gacatccgcg agatccggga gcgcgtcgtc ctccagccgg ccgaggggcg ctacaaggtg
     4621 tacatcctcg acgaggcgca ccagctcacc gatgcggcct ggaacgccct gttgaagctg
     4681 atcgaggagc cgccgcctca cctcgtcttc gtgttctgca ccaccgatct ctccaaggtg
     4741 ctgccgacgg tgcgctcgcg ctgccagacg ttcgtcttcg cgcgcccgcg cctgcccgag
     4801 ctcgtgcgcc tgctgcgccg cgtcgctgac ggcgagggca ttcaggttcc cgaccaggca
     4861 ctcgcgctcg ttgcgcgcgg tgcgcgcggc tcgttccgcg acgccgtctc cacgctcgac
     4921 cagctcgcgt cggcaaccga gaacgagatc accgtgcagt ctgtgttgca gttgctgggt
     4981 gcggtcgaag aggaagcgct gttccgcttg tgtgacctcg tcgtcgaccg cgacaccgcc
     5041 ggcgcgctga cgttcgtcga ggagttgtcg gaacagggac aggacctggg ccggctcgtc
     5101 gtcgacctgc tcgagcatct gcgccacctc atgctcgtgc agcacatggg cgaagtgccg
     5161 gacacgctcc cggtcacgga agagacgcgc gagcggctgc gggcgcaggc gaaccaactc
     5221 ggcgaggcga ccgtcatccg cctggtcgac ctcctggccg tcgcgatcga ggacatgcgg
     5281 cagggcggcg acccgcgcct cccgctcgag ctcgcgctcg tgaaggtgac gcgccccggc
     5341 gccgacctct ccagagaatc cctctcgtac cggctcgaac agctcgagac gcgtggccat
     5401 gtgccgacgg cccctgcctc ggggactgtt cctgagacga gtcccgaaca ggcacctgtc
     5461 gaggcggtcg cgctggccgc gccgccctcg ctcgagctcg agcagctcca ggaggcctgg
     5521 cagcggacga tcctgccggc cgtcgaggaa cgctcgattc cggctgcgtc cgtcttgcgc
     5581 gaggcgcacc ccgcagagct ggcgaacgac accctcacgg tcgagttccc ctcgagcgcg
     5641 gccttccacc ggcagctggc ggaggagccg aagaacgcca cgctcctcgc cgacgcgctc
     5701 tacgaagtca ccggacgccg ccttgggctc gcgtttgccg agggcgaggc gcgggccgac
     5761 gcggtcgaag acgacgagcc tgcgggtgag gagaagattc tcgagctcgt gaaagagacc
     5821 ctggacgccc acgaacggga ggactagcga tgggcatgga catgaatcag ctgtttcaac
     5881 aggtcggcca gatgcaggaa cagatgcaga aggctcaagc agagctggcg aacgagacgg
     5941 tcgaagccac ggccggcggc gggatggtca ccgtcgtcgc gaacggcgcc ggcgagatca
     6001 ggcagatcaa gatcgatccg aaggcaatcg acccgagcga ccctgaaatg ctcgaggaca
     6061 tgatccttgc cgccgtgaac gaggcaattc gtgcagctca gaacctgatg gagtccaagc
     6121 tcggcggcct tgcgggcggt gcgctcgggg ggctcggtct tccgggacta ggcgagtaat
     6181 gcgcgaccgc ctcggtgcgc ttccgctgtt cgccgatctc gacgacgctc agctcgaccg
     6241 gctcgccgca gcaacgacgg agttcgaagc gccggccggt caggcgctga tcgaacgcgg
     6301 ccgtgcaggc tcagggatgt tcgtgctcga ggagggggcg gcagtcgtcg aggcgccgga
     6361 aggaacacgc gagctcgggc ctggcgacgt cttcggcgag cgcgcgctgc tcggcgagga
     6421 catcgagcgc actgcccgag tacgggcacg gaccgacgtg cgctgcctcg cgatcgcgcg
     6481 gcccgagatc gaacggctcc tcgcggagga tccgcgcgtc gccgcccgcc tcggtcagct
     6541 ctcgacctga ggccctccgt agcctcggca atccgaggca tgtcggcaca ataggacagt
     6601 gctcagcccg gctgtggaaa acctggtcgc ccagctctcg cgcctgcccg gcgtgggatc
     6661 gcgcacggct cacaggctcg cgttccatct tctgcagcga ccgacggacg aggcgctcgc
     6721 gttggccgct gcgatcgagg aggtcaagga gcgcgttcgc ttctgcgacg agtgcggcaa
     6781 cctgaccgag gaggagacgt gcgcgatctg ccgggacacg cgacgcgacc acaccctcat
     6841 ctgcgtcgtc gagcaaccgg tcgacctcat ctccgtggag aagacgagcg agtttcgcgg
     6901 tctctaccac gtcctcggcg gcgcgctctc tcctctcgac ggcgtcgagc ccgaacacct
     6961 tcgcatcgac gagctgctcg gccgcgtcga acgaaacggc gtgctcgagg tcgtgctggc
     7021 gaccaacccg aacatgaccg gggaggcgac cgctgcctat ctcgcagacc ggttgcgcgg
     7081 acgtgtgcgc gtgacccggc tcgcgagcgg actgccggtc ggcggcgatc tcgagtacgc
     7141 agacgaggtc acgctcggcc gcgcgctcgc aggccggcgg gagatggaat gacgacgatc
     7201 agctacgagg ccgttccagc cgtcgaaggt gacgccgcag ccgcgcccgg aaacacgctc
     7261 gtgatgaagt tcggcggcac gtcggtcggc gacacgcaga agatcaggga cgtcgcccgc
     7321 cggctcgtga ccgcccggga ggctggacaa cgtgtcgtcg gcgttctgtc ggcgatgggc
     7381 gacacgacgg acaacctgat tcggctcgca cacgagatgt cgccgaagcc tcatccccgc
     7441 gagtacgaca tgctcgtgtc cgtcggcgag cgcatctcga acgccctgtg cgcgatggcg
     7501 atccgcgatc tcggacacga agccgtctcg ctcaccggct cgcaggctgg aatcgtcacc
     7561 gacacgttcc atgggaaggc gaagatcgtg gaggtgcgtg cgcggcggtt acacgccgag
     7621 ctcgatcgcg accagatcgt gctcgtcgcc ggcttccagg gcgtgtcgac ggagctggag
     7681 gtgacgacac tcggccgtgg cgggtccgac gcgaccgccg tcgctctcgc ctctgcactc
     7741 ggtggagcgt gcgagatcta caccgatgtt gaaggtgtct tcaccgccga cccgcgggtc
     7801 gtagccgacg cgctgaagct gccgaagatc tcgtacgagg aaatgctcga gatggccgcg
     7861 acgggcgctc gtgtgatgat ggcgcggtcc gtcgagatcg cacgcaggta cggtgtgcca
     7921 ctccacgtgc gctcggcgtt cggcgacagc gaggggacgt ggatcaccaa ggaggagaac
     7981 ctggtgctcg agaaggcgat catctctgga gtcactcacg acacgtcgga ggcgaaggct
     8041 acggtgctcg ccgtgcccga ccgtcccggt atcgccgcac gcatcttccg cgcgctggca
     8101 gacgccgggg tcaacatcga catgatcgtg cagaacatct cggcggaagg cgcgaccgac
     8161 atctcgttca cgctcccgaa gacggacttg ccggcggcgg agccgatcct cgatgtcctc
     8221 gcgtccgaga tcgccgcaaa gggtgtcgtc tacgacccgg acgttgcgaa ggtgtccctc
     8281 atcggtgccg ggatgaagag ccatcctggt gtcgccgccg acatgttcga ggctctgggc
     8341 gaagccggga tcaacatcga gatcatctcg acctcgtcga tccgcgtctc gtgcgttgtg
     8401 cgcgccactg aggtcgagcg tgcggtgcag gcggtgcacg accgcttccg gggctacgcc
     8461 gaggagcgcg tgggtgcctg agccccgcat cgcagtggtc ggcgcgaccg gcgctgtcgg
     8521 cgccgtcacg ctggagctcc tgagggagcg cggctacggg caggtgcggg cgcttgcttc
     8581 cgcgcgatcg gccggaaagg aattggccgg cggcggcgtc gtcgaggagg cgacgccgga
     8641 ggtgctcgct gccggcgaca tcgacctcgc gttcttctcc gtcgggacga gcgcgtctcg
     8701 ggagctcgtg ccgcatgccg ttcgcggtgg ggccgtcgcc gtcgacaagt cggcggccta
     8761 ccgcctccag gagggcgtcc ccctcgtcgt gcccgaggtc aacggcgaac gcgctcgcga
     8821 gcacgacggc atagtcgcga atccgaactg ctgcgcgatc ccgctcacga tggcgttgaa
     8881 gccgctacac gacgccgctg gtctcgcgcg cgtgcgcgtg agcacgtatc agtcagtgtc
     8941 cggcgcgggt gcgcagcgta tggaagagct gcgtgccacg ccgtccggcg acggcaacct
     9001 cgcgatggac tgggacttcg acggcgagga gttcgacgag gagtccaagc tgcgcgacga
     9061 gactcgcaag attctcgagc tgcccgagct gccgatcagc gcgacgtgcg tgcgcgtgcc
     9121 ggtgctcgtc ggccacgcgg aggcggtgtg ggtggagacc gaagggccgc tctcggcaga
     9181 cgacgcacgc gggatactcg gcgcagcacc gggcatcaag ttggagggct tccccacgcc
     9241 tcgcgacgca accgggatcg acgagatcct cgttggtcgc atccgacgcg acccaaccgt
     9301 ggacaacgga cttgtcttct tcgtcgccgc cgacaacctc cgcaaaggcg ccgcgctcaa
     9361 cgcgatccag atcgcggagc tgatcctgca gcgccgcccg gtcgccgcct gagcaactgg
     9421 gcctggctcg ctgtcgcact cggcgcaacg gcggccctgt ggttggtgtc gatcttcgtc
     9481 ttgctcctgt tcggccgccg aagcgatgct cgtgaggtcg cgcgcttcct gccggactgc
     9541 atcgtgctcc tcaagcgtct gcttcgagac aacagggtgc cgcgacgggc caaggtcgtg
     9601 ctcggactgc tcgtcctgta cctggtcatg ccgctcgacc tggtaccgga cttcattccg
     9661 gtcgccggcc agctcgacga cgcagttctc gtcgccgccg cgatcgccta cgtcgcccgc
     9721 agcgccggac gcggcgtgat cgaagagctc tggccgggct ccgagcgagc tctgaacgtg
     9781 gtactcgcgc tcgcttgatc gcctcgccct ccggcgtccg cagccgccgg cgggccagaa
     9841 gtgcgagagc ccggggggac gtcgggctct cggacgagcg gtcagcactc tgctagggct
     9901 tcctcagcat ctagttcgta gacacacggg gcaagggctg cgagacctgt ctttcgttga
     9961 gccttctcaa cacgcccacc gcagtcgccc acactgcggt caaggcccag gcggtaccgg
    10021 cgcaggagcc ggagtgaacg cctccttgac cgcgcgcatc accttctccc gctttccctc
    10081 caggaccggg agcagcccgc cgaactcgag gcgcgccagc tcgacgtggc cgaagcgtcg
    10141 cagcgtctgg aagccgaagt cggcgagaaa atccgaagga aagatcgtct tgtgcaccag
    10201 gaagcgctcg tactgggact ccccttcgcg ataccggtac gcgaacgtct cgagcgcctt
    10261 cgcgccacga tcgcgtgcat cgccgatcgc cgcgaggaaa agcgactgca tcacccacgg
    10321 gctcgaggtc tcgacgagat acgcgcacgt caccagcacc gcgtcgtccg acggcggtcc
    10381 ggccggaagc tcggccgccc gcgggaacaa cccggcgggg ccgtactgca tcgagccgag
    10441 gacgtgcccg tccccgtcgt agtacacggt cccccatggc ccccactcgc tctcggcctt
    10501 ctcgatccac ttccgcttgt ccgtcgtgcg accctcgcgg gactgccacc agacgcagtc
    10561 gtggcacacc gtcggtgcgt cctgcaacgt cacgcccgtc aaacccgtga cgcgcggccc
    10621 ggtcacggcg gcccgatctc gtcgacgatc acgtcgaccg cctctggatc gactttggcc
    10681 atgcgctcga ggtaggccgc gacacggtgt tgcacttccc ggccgacctc cggaatcgac
    10741 accccccact ccacggcgag atgtaactcg acccgcacac gcccgtcttc atcgacgatc
    10801 cgcacgccct tgcgcctgtg cagcgaactc tcgaccaggc ctcgcacccc ctcgacctcg
    10861 cgcgccgcat cggcggcata gctcgcgagg atgtcgctcg agatcgtcgc catcacggag
    10921 gacgttttag caccagttcg gttgcttcgg gccgcgcgaa gaaccggaac ggaacgaacg
    10981 tggttccgag acccggcgag acgtgcatcg tgccgcccgg aagttcatag agtcctgtcg
    11041 cgaaacgcac gctcggatgg gcgaagcgga acttgcggtt acgtccgagc ggaagggcga
    11101 tctggccgtc gtgcatgtgc ccgctgagca cgagatcgaa cgctccgggc ggcaggaagc
    11161 cgaggacgta agggaagtgg cagagcagga tgcgcaggtc cgcggactca tcgatgagct
    11221 cccacggccg cgaacgcccc tgccgatacg cacgcgggtc gacacccacc agctgcactc
    11281 ggtgcccgcg tagctcaacg gtctcgctct cgtcgacgag caagcgagcc ggagtcagat
    11341 ctgtgagccc tgcagcccgc gagaaaggat cacgactgtg ctcgacgtcg tgattcccga
    11401 ggacgacgaa cggccgctcg aggcgcccga gctgctcgcg cagcaacgct tcgccgcgcg
    11461 ggcgcgagac gaggtcaccc gtgatcgcga cgagatcggg cttacgctgc tccacccagt
    11521 cgatcgcccg ctcgaccgcg accgtaccgc gcgagggcag tccgaagtgg aagtcggaga
    11581 ggtgtgcgat ccgcaggccg gccaactctt ccgacaggcg agccagggga acgtcgagcg
    11641 tccgcagccg cacccacccg gcctcgaacc agccccagcc gaccgcagct ccggcgacgc
    11701 cgccgagcag gctcccggcg cgagtcagac ttcgagcttc tcgaggaccg gggtgaccga
    11761 gacgacgtgg cgcttgaact tcagctccga ctcctccagc ccggccgcga cgcccaccag
    11821 ttgcgagagg tgcaggatgg gcatcgcgaa gtccttgccg gtcgccttct tgagcttgga
    11881 ttgccacgcg tccagcgaaa ggtggcagag cgggcacggg gtgacgatca cgtccgcccc
    11941 ggcctccttc gcctgttcga tcggctggat cagctcgccg agcgcggtct cctcacgggc
    12001 ctggatgatc gggaacccac agcacttgat cttcgccgga tagtcgatcg cctcgccgcc
    12061 gcacgcctcg atgatccgct cgagcgacca gggacggtcc ggatcttcga agccgaggat
    12121 cttcgagggg cgcaagatct gacaaccgta gaacggcgcg atcttcagcc ccttgaggcc
    12181 cttgtgcgct gactgcttca gcagttcgta cccttcgccc tcggcgatct cccacaacag
    12241 atgccgcact tcgacgttgc cgctgtaggg cgggaccccc acgcgctcga ggttggtgct
    12301 gacgcggtcc agcaggttct cgtcgttctt cagctgccag ttggcctgcc tgagattcag
    12361 cgtgcagacg ttgcacacgg tcagcagcgt gtcgcaaccg gccgcctccg cgtacgcgag
    12421 gatccgcgcg ttcaagtgca ggtagtagtc cggctccgcc tcgtggatgt caccggcccc
    12481 gcagcacgtc actgtctcga gctctacgag ctctagacca actttcggag caagagcctg
    12541 ggtggatgag tccagctcct tcgccgagag cgacgccaga cagcctttgt agtacgcgac
    12601 gcgcctcatg tcgtttccgt ctcggtggtc ttcacgccgg cttgctccga gaactcgatc
    12661 ctgcccagcg catgctcgcc ctggacgatg ccgagtgccc cgtcacggcc ctgctccttg
    12721 accaggtggt agagcttgcg agcctcgcca acccgatctg caacgtgcgg cgggaacggc
    12781 ggcggcacct tgccgtgctt tacgaggctg agcgcgaacc tcgtctgctt gatcgcctcg
    12841 acgacaccct gagtcttcgg gacgagctca gtctcgcgca accagccggt ggtcttggcg
    12901 gaagtgacga accactgcgc gtgcttcgcg cccatatcgt gatcgatccc ttccttgatc
    12961 gactcggccc cgagcttcgc gatcgcatcg cggggatcga cgcctttcgg acaccgctcg
    13021 ttgcagaagt agcagcgcgt gcagtcccag atgccgtgct cgtcgttgta gtcgttcagg
    13081 cgcgccacat ccgcctggtc gcgcacgtcg ccgacgaagc gcatcccttt cgcgagcgca
    13141 gccggcccga agaagtccgg atcggactcc atcgagttgc actcggagac gcagcagccg
    13201 cacatgatgc agagcgcctc tttgtggatc acgttcatct gctgctgcga gacgatgcgc
    13261 tccttctcaa gcggctcctc atagcccggg tcgagccagg gcttcatccg gcggatcttc
    13321 ccccagaagg gatccatgtc gacgacgaga tccttgatca cgggcatgtt ccccatagac
    13381 gagatcaccg gcacgtgacc ggacttcgcg atgtcgtaca tgcgcgtctt gcaggcaagg
    13441 accgccgcgc cgtccatgcg cattccgcac gagccgcaga tcatcatgcg gcagctcttg
    13501 cgatacgcga gcgtgccgtc gtgcttgtcc ttgatgatgt cgagcacgtc gaggagggtc
    13561 acccagtccg gcgcctcgac ctcgtagcgc ttcagctcct tctcgccggt ctcggcgtcg
    13621 taacgccaga tcttcagcgc gacgtcgatc gtcgtcgagc ctggtgccgg ggctagctcg
    13681 ctgtgcacta ataggtcctt tcctggggct tccagttggt catggtcacg ggcttccagt
    13741 cgactctcgg ctcgccgtcg gcccaactga cgagcgtgtg cttcagataa tgctcgtcgt
    13801 cgcggtcggg atagtcatag ggtcgcgcat gcgcgccccg gctctccttg cgctcgatgc
    13861 cgcacggaac catgcacgcg gcgaggtcga gcaggaagcc gagctcgagg gcctgggtga
    13921 ggtcgttgtt gaagacgtta cccttgtcct cgacgaccac gctctcgtag cgttcccgca
    13981 ggcctgtgac gatctccccc tgctttcgca tctgctcctc gcggcggaag acaccgaagt
    14041 tctcgtgcat cgactccgcc agctcatcgc ggatcgacca gggccgctct ccctcacgtc
    14101 ggtcgagcag ctccttcagc tcacgctccg catcggcttc gacggacggc ggcacttcga
    14161 ccgtcgtgtt cgagagcgcc cagtcggcgg cgtggcggcc gacgcgcttc ccatacgtga
    14221 tcgtctccat cagcgcgttc ccgccgaggc gattggcgcc gtggaccgac acgcacgcgc
    14281 actcgcccgc ggcgtagagg ccctcgacct ccgtcaggcc ccagacgtcg gtgtcgacgc
    14341 cgcccatgtg gtagtgggct cccggccgga ccgggatcgg ctcgtagatc ggatcgaccc
    14401 cggcgaaggt catcgagagc tcgcgcgtgc cgtgcagccg ctccaggatc ttctccgcgc
    14461 cgagatgccg caggtcgagg atgacgttgc cgttgatccc ccggccctcg tcgatctccg
    14521 tctgttctgc gcgcgagatg acgtcgcgcg acgcgagctc catcgcgttc ggcgcgtagc
    14581 gtttcaggaa ccgctcgccc tcggagttga ggaggtacgc gccttcgccg cgcgtgccct
    14641 cggtgatgag gacgcctgtt ggggagagcg tcgtcgggtg gaactgcatc atctccatgt
    14701 ccttcagcgg gacgccgacg cggagcgcga gggccatgcc gtcgccggtg caggcatagg
    14761 cgttcgtggt tccgacgtac agccggcccg ccccgccggt cgtgaggatg accgtcttcg
    14821 cgccgatcga cttgaggccg ccgttgagca gatcccaggc gatcactccc tgacagcggt
    14881 cgtcgttcat gacgagcttc caggcgaaga actcctcgta ggtcgggatc tcgcgcttca
    14941 tcacctgctc gtagaggaca tgcacgagca catggcccgt gatgtcggcc gcataagccg
    15001 tgcgcggcgc gccggcggcg ccgaaaggac gctgcgcgat gcggccgtcg tcggtgcgtg
    15061 agaagaccgc cccccagtgc tcgagctggt agacgtcgcc gggcgcctcg ttgcacagga
    15121 tctcgatcgc gtcctggtcg cccaggtaat cggagccctt gaccgtgtcg tacgcgtgct
    15181 ctgcgggact gtcctccgag gcattcccca gagcggcgtt gatcccgccc tcggccgccc
    15241 ctgaatggct gcggacggga tggatcttcg agagcagcgc cacgtcggcg cccgcatcgt
    15301 gcgcctcgat ggccgcgcgc attcccgcgc agccggcacc gacgatgagg acgtcgtgct
    15361 tggcctccac cacagaggaa gctatcgaat ggacgctagt tgcggacttc gctacgaagg
    15421 ttgcgaagag cggcaggcac tcgagagcac ccgggggtcg cggcgggacc gcgatcgacc
    15481 cgaggttgtc gcgcaactct tcggccgccg ccgcgccgtg ggtccgctgg catcgacgcc
    15541 tggcggccgt cctcgctttc cttaagtaag cgaggccgtc gataagatcc ccgagtccca
    15601 gccatcgaaa aagggagctg accagcgtgg cgaagatgct gttccgactg ccgcaggcga
    15661 agcgggtgat cgagaacccg agccctgagg aagtcaagga gctcgcagcg aagatgccga
    15721 ccgcacgccc gacgaagtac ggcaacctga acgtgcagac gcaggtgctt gcgcgctcca
    15781 agcgctctac attcctcgtc acagacgagc ctgatggtca gaaccaggcg ctctcgaccg
    15841 acgacgcggc caggtgggct gcgcgtcagg atgagtacat cgccggccgg gagatggttg
    15901 tgatcgacgg ctatatcggc accgacctgg agttccgcac ctcgacgcgc ctgtatgtcg
    15961 aggcggcgaa cgcgaacatc gccggaatgc agcagcagct ctatttcggc ccgccggagg
    16021 aggacttcga gcccgagctg accgtcatct acacgccgaa cctgaaggcg gagggcttcc
    16081 cggacgaccg gctgatcatg gtcgacctcg agcagggtgt gacgcgcgtc ttcaactcgg
    16141 attacttcgg cgagtcgaag aagggcgggc tgcggatgtg gaacaagctc gtctacgacc
    16201 agggcggcct ccccctgcac gccggctgca agatcatccc gacggacgac ggcgacaagg
    16261 tctgcctgac ggtcggcctt tccggcaccg gcaagaccac cacgactttc acgaagcaga
    16321 acggctcgct tcccgttcag gacgacttcg tcgcatggat gcccgacggc ggcgtctacg
    16381 tgaccgagaa cggctgcttc gcgaagacct acgggctcaa ccccgaggac gagcccacga
    16441 tctacggcgc ggtcacgcag ccgaactcgt atctggagaa cgtctcgcaa aagggtgccg
    16501 agctcgactt ctacgacgag agctacacgc agaacggacg cgcgacgttc ccgttcggcg
    16561 taatcgagtc tgccgccgat cgcgagatcg acgacgcgca cttcctgctc atcctgaatc
    16621 gcaacgagaa tgtcatcccg gcagtcgcga agctcgaggg acctcaggcg gcggcctact
    16681 tcatgctcgg cgagacgaag ggaactgcgg ccggcggtgc ggacgaagcc gggaagtcac
    16741 tgcgcgtgcc gggaaccaac ccgttcttcc cgatgctgca cgacttgcag ggaaaccgct
    16801 tcctcgagtt gctcgaggag caccccctcg aggtgtatct catgaacacc ggacgcgtgg
    16861 gcgggcccga agcggacgag cgctcgaaga aggtgcgcat caagcactcc tcggcgatcg
    16921 tgaagggcat cgccgagggc gcgatcgagt gggagcgcga tcccgacttc ggctatctcg
    16981 tggcgtcggc cgtccccggc atcgacgacg tcgaagtgct ccagccgcgc aagctctacg
    17041 agcgaaccgg acgcgcggac gagtatcgcg cgcaggtgga acggctcaag tcggagcgca
    17101 cggagttcct cgggaagttc ggcagtctct ccgccgagat cgtcgctgcg gccagctaag
    17161 ccctgttcct agttccctcg gaagagggtt ataggccgaa tggcctaccc cgataagggg
    17221 gccatgggac tcgcaaggca caatcccagc ggccaggcga ggcttggcct ctccgcaact
    17281 gctg
//
LOCUS       DASVEU010000203         5837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1694920,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000203 DASVEU010000000
VERSION     DASVEU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694920"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VF225_08490"
     CDS             <1..824
                     /locus_tag="VF225_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145332.1"
                     /translation="RGDARAGRTRRDARLMADLLRAENVRKEFGGLVAVNDLDFTIPD
                     RSIVSLIGPNGAGKTTFFNMLTGVYRPTGGEILFDGHDVTSKPPHTITKLGIGRTFQN
                     IRLFPQMTALENVLVGMHCRLRGGILGSIFGTPRVRREEREAHARGRELLRYSGLGRV
                     DDELAENLSYGDQRRLELARALATEPKLLLLDEPTAGMNPQETARFTAFLGKLLEDRP
                     ISILLIEHDMKVIMGVSERVTVLDYGEKIAEGTPAEIQRNERVIEAYLGKAATAS"
     gene            821..1549
                     /locus_tag="VF225_08495"
     CDS             821..1549
                     /locus_tag="VF225_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145333.1"
                     /translation="MTDNGALLRVEDVHTYYGSIEALKGISIEVHEGEIVTLIGANGA
                     GKSTTLRSINGLNHPRRGSIHFRGKNITREAPHDVVRLGISQSPEGRKLFPRMSVLEN
                     LEMGAFQRHDRAELRSDLDRVYSLFPRLSERKQQRAGTLSGGEQQMVAIGRALMAHPT
                     LLLLDEPSMGLAPIFVERVFETIVEINHQGTTILLVEQNAMMALDVASRGYVLETGTV
                     ALADDAKALLRNEQVRKTYLGETD"
     gene            1566..2441
                     /locus_tag="VF225_08500"
     CDS             1566..2441
                     /locus_tag="VF225_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145334.1"
                     /translation="MSRSKLTVLAGALIVLLALVAGGCGGGNKSAATTAATTTSASSI
                     QAPASIKSSGTLAFCSDITYPPEESYKAGTTTPEGSDIDIGTYIAKKMGVKARFDNTG
                     FDGIIAALLAKKCDAVISGMNDTPERKKQVAFVDYLKVGQSFMVKSGNPENITGIASL
                     AGKAASVETGTTNKDYLDAQSKKLQAQGKKGIKVVTFPKDTDAANALKTGKVDAYFGD
                     APVVAFYIEKDPSSFSIGGTPVNPISIGIALRKSDTELQAAVKQAVTAAYADGTMDKI
                     LTRWKLGKAVAKLQP"
     gene            2445..3239
                     /locus_tag="VF225_08505"
     CDS             2445..3239
                     /locus_tag="VF225_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7145335.1"
                     /translation="MLAAIDWHILWARIFHPDHVFVLALWRTIYISVCAQTLGVVLGL
                     IAALMRLSRIAPLRWLSGLYVLVFRGTPVIVQIFFVYFGANLLFGFTLIPRTVNFGLF
                     QMDGAAFAGIVALGVNEGGYMREIIRAGIDAVDKGQMEAAKSLGMPYRLAMRRIVLPQ
                     ATRFIIPPLGNEFNNMMKTSSLVFFIGVTELFGDAEIRYSTTFKPVEYFLAVAFWYLV
                     LTSVWSLIQAQIERKLAVSERGDELSFRERVAAAWLPSAMRIRGTP"
     gene            3236..4009
                     /locus_tag="VF225_08510"
     CDS             3236..4009
                     /locus_tag="VF225_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7145336.1"
                     /translation="MSSEGPVMRAEGVVKRFGRLEVLKGVSLDVNPGEVVCIIGPSGS
                     GKTTFIRCINHLEKIDSGRVEVNGHLIGYRERSGKLVEDSERNIARQRAEIGMVFQRF
                     NLFPHKTALENVIEAPVHVRGTAKDEAIATGQALLERVGLGDKFDTYPGKLSGGQQQR
                     VAIARALAMNPAVMLFDEPTSALDPEVIGEVLDVMKALALDMGTTMIVVTHEMGFARE
                     VADRVVMMDDGAIIEEGSPKHFFDSPTHERTKRFLSKIL"
     gene            complement(4006..5475)
                     /locus_tag="VF225_08515"
     CDS             complement(4006..5475)
                     /locus_tag="VF225_08515"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX7145337.1"
                     /translation="MTADDDAIVLRPPLPADSVADVGAAVRDALRFPLAGEPLEALVS
                     RGSRATIVVEPPHLPLPGAERDPRQAAIAAAVAELERLGIPTGYQTLLVAGGLTRRAG
                     NEQLETLVSPEFARRFHGHVEVHDVERDDLVELGTAGSIPLRVHPALVHTDVVVVVTA
                     AETVLHGGPAAFVGAAGTEVLRAAGAYSLLETSASQGWRLGLELERALARRVPVIGAS
                     LVLNHPRFGGTLGGYPYEEQALERVVRSPFRHIFRALPEALRAYVLRSVPLERTVAAA
                     FAGPPSVAHSEALLRGVELRGLTLDGQLDAICIGVPRTTPYLPRERPNPLLGAYLGLG
                     LALRLWRDSFPIAEGGTAILFDRFQRHFSHPTQQPYRAFFAATRATGPDNAVLAEAEG
                     LAAADPRALEAYRGGRSVHPLLPFVDWDGCRPALGRLGHVIIAGCRDHAAARQLGFVP
                     THGVGAALTMARGWTERPPRVGILLSPPYFPLRVSSGSS"
     gene            5601..>5837
                     /locus_tag="VF225_08520"
     CDS             5601..>5837
                     /locus_tag="VF225_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145338.1"
                     /translation="MPKTLTGAELQLDDSPVRDAELFLVDGNNLAYRAFFALPEELAT
                     TEGFPTNALLGFTNMLFKLLSDYKPKGVAVAWDSS"
BASE COUNT         1081 a         1874 c         1979 g          903 t
ORIGIN      
        1 gaagagggga cgcgcgagcc ggaagaactc ggagagacgc gcggctgatg gccgatctac
       61 taagagcgga gaacgtgcgc aaggagttcg gcgggctcgt ggccgtcaac gacctcgact
      121 tcacgattcc cgaccggtcg atcgtgagcc tgatcggccc gaacggcgcc ggcaagacga
      181 cgttcttcaa catgctgacg ggcgtatacc ggccgaccgg cggcgagatc ctgttcgacg
      241 ggcacgacgt cacgtcgaaa ccgccgcaca cgatcacgaa gctcgggatc ggccgcacgt
      301 ttcagaacat ccggctcttc ccgcagatga cggcactcga gaacgtcctc gtcggcatgc
      361 actgccgcct caggggcggc atcctcggct cgatcttcgg gacgccgcgc gtccggcggg
      421 aggagcgcga ggcgcacgcg aggggtcgcg agctcctccg ttactcgggg ctcgggaggg
      481 tggacgacga attggcggag aatctctcct acggcgacca gcgccggctc gagcttgcgc
      541 gcgcactcgc gaccgagccc aagctgttgc tgctcgacga gcccaccgcg ggaatgaacc
      601 cgcaggagac cgccaggttc acggcgttcc tcggcaagct gctcgaggac cggccgatca
      661 gcatcctcct gatcgagcac gacatgaagg tgatcatggg agtctcggaa cgcgtcaccg
      721 tgctcgacta cggcgagaag atcgccgagg gaacgccggc cgagatccag cgcaacgaac
      781 gcgtgatcga ggcgtatctc gggaaggcgg cgacggcgtc atgacagata acggcgccct
      841 cctccgcgtc gaggacgtcc acacgtacta cggctccatc gaggcgctca aaggcatctc
      901 gatcgaggtc cacgaaggcg agatcgtcac cctgatcggt gccaacggcg ccgggaagtc
      961 gacgacgctc cgctcgatca acgggctcaa ccacccacgc cgcggctcga tccactttcg
     1021 cgggaagaac atcacgcgcg aggctccgca cgacgtcgtc cgccttggga tctcgcagtc
     1081 tcccgagggc cgcaagctgt tcccccgcat gtcggtgctg gaaaacctcg agatgggtgc
     1141 cttccagcgc cacgaccgtg cggagttgcg gagcgacctc gaccgcgtct attcgctctt
     1201 cccgagactc tccgagcgca agcagcagcg cgcggggacg ctctccggag gcgagcagca
     1261 gatggtcgca atcggacgcg cgctgatggc gcatcccacg ttgcttctcc tggacgagcc
     1321 ctcgatgggg ctcgcgccga tcttcgtgga gcgcgtcttc gagaccatcg tggagatcaa
     1381 ccatcagggc acgaccatcc tgctcgtgga gcagaacgcg atgatggcgc tcgatgtggc
     1441 cagccgcggc tacgtgctcg agacagggac ggtggcactt gccgacgacg cgaaggcgct
     1501 tcttcgcaac gagcaggtcc gaaagacgta tcttggggag accgattgac gaaaggagcc
     1561 ccactgtgtc ccgctcgaag ctgactgtgc tggcaggcgc cctcatcgtc ctccttgccc
     1621 tggtcgccgg tgggtgcggt ggaggtaata agagcgccgc aacgacggcc gcaaccacga
     1681 cttccgcgtc ctcgatccag gcgccagcct cgatcaagag ctcgggcacg ctcgccttct
     1741 gcagcgacat cacctatccg cccgaagagt cgtataaagc cgggaccacg acgcccgagg
     1801 gctccgacat cgacatcggc acctacatcg cgaagaagat gggcgtgaag gcgcgcttcg
     1861 acaacaccgg cttcgatggg atcatcgcgg cgctgctcgc gaagaagtgc gacgcggtca
     1921 tctccggaat gaacgacacg cccgagcgga aaaagcaggt cgcgttcgtc gactacctga
     1981 aggtcggcca gtccttcatg gtcaagtcgg ggaacccgga gaacatcacg ggcatcgcaa
     2041 gccttgcggg caaagcggcc tctgtcgaga cgggcacgac gaacaaggac tacctggacg
     2101 cacagagcaa gaagctccag gctcagggta agaagggaat caaggtagtc accttcccaa
     2161 aggacacgga cgcggcgaat gcgctcaaga cgggcaaagt cgatgcgtac ttcggcgacg
     2221 cgcctgtggt cgctttctac atcgagaagg atccctcatc gttctcaatc ggcggaactc
     2281 cggtcaatcc gatttcgatc gggatcgcgc tccggaagtc cgacaccgag ctccaggcgg
     2341 ccgtgaagca ggcggtgact gcagcgtacg ccgacggaac gatggacaag atcctgacga
     2401 ggtggaagct cgggaaggcc gtcgcgaaac tgcagccgtg atcgatgctc gccgcgatcg
     2461 actggcacat actctgggca cggatcttcc accctgatca cgtcttcgtc ctcgccttgt
     2521 ggaggacgat ctacatctcg gtctgtgccc agacgctcgg cgtcgtgctc ggcctgatcg
     2581 ccgcgttgat gcggctgtcc aggattgcgc cgctgcgctg gctctccggg ctgtacgtgc
     2641 tcgtctttcg cggaacgccg gtcatcgtgc agatcttctt cgtctacttc ggcgccaacc
     2701 tgctcttcgg cttcacgctc atcccgagga cggtcaactt cgggctcttt cagatggacg
     2761 gagccgcgtt cgcgggcatc gtcgccctcg gcgtcaacga ggggggctat atgcgcgaga
     2821 tcattcgcgc ggggatcgat gccgtcgaca agggccagat ggaggcggcg aagtcgctgg
     2881 gaatgccgta ccgactggcc atgcgccgca tcgtcctgcc gcaggcgacg cgcttcatca
     2941 tcccaccgct cggaaacgag ttcaacaaca tgatgaagac gtcctcgctg gtgttcttca
     3001 tcggggtcac agagctgttc ggtgacgccg agatccgcta ctcgacgacc ttcaagcccg
     3061 tcgagtactt cctcgccgtc gcgttctggt acctcgtgtt gaccagcgtg tggagcctca
     3121 tccaggcgca gatcgagcgg aagctcgccg ttagcgagcg tggcgacgaa ctcagctttc
     3181 gcgaacgcgt ggcggcggcg tggctgcctt cggcaatgcg aatcaggggc acaccatgag
     3241 ctcggaaggg cccgtcatgc gggcggaggg cgtcgtcaag cgcttcggtc ggctcgaggt
     3301 cttgaagggc gtgtccctcg acgtgaatcc gggcgaggtg gtctgcatca tcggcccgtc
     3361 gggttcggga aagacgacat tcatccgctg catcaaccat ctcgagaaga tcgactccgg
     3421 ccgcgtggag gtgaacggcc acctgatcgg gtaccgcgag cggagcggga agctcgtcga
     3481 ggacagtgag cgcaacatcg ccaggcagcg ggccgagatc ggcatggtct tccagcggtt
     3541 caatctcttc ccgcacaaga ctgcgctcga gaacgtgatc gaggctccgg tccacgtccg
     3601 tgggacggcg aaagacgaag ctatcgcgac tgggcaggcg ctgctcgagc gggtcggcct
     3661 cggcgacaag ttcgacacgt atccggggaa gctgtccggg gggcagcaac agcgggtcgc
     3721 gatcgcacgg gcgcttgcca tgaaccccgc ggtgatgctc ttcgacgagc cgacgagcgc
     3781 tctcgatccc gaagtcatcg gcgaggtgct ggatgtgatg aaggcgctcg ctctcgacat
     3841 gggtacgacg atgatcgtcg tcacgcacga gatgggcttc gcgcgcgaag tcgccgaccg
     3901 cgttgtgatg atggacgacg gcgcgatcat cgaggaaggt tcgcccaagc atttcttcga
     3961 ctccccgacc cacgagcgga cgaagcggtt cctctcgaag atcctctaac tcgagccgga
     4021 actcactcgt agtgggaagt aggggggcga gaggaggatg ccgacccgcg gcggccgctc
     4081 ggtccagccg cgcgccatcg tcagcgccgc cccgacgccg tgtgtcggca cgaagccgag
     4141 ttgccgggcg gcggcgtgat cgcgacagcc ggcgatgatc acgtggccga ggcggccgag
     4201 cgcaggccgg cagccgtccc aatcaacgaa ggggagcagc ggatggacgg agcgtccgcc
     4261 gcggtaggcc tcgagcgcgc gaggatcggc agcggcaaga ccctcggctt cggccagcac
     4321 agcgttgtcg gggcctgtgg cccgcgttgc cgcgaagaag gcacgatacg gctgctgcgt
     4381 ggggtgtgag aagtgacgct ggaagcggtc gaagaggatc gccgtgccgc cttctgcgat
     4441 cggaaacgag tcgcgccaga gtcgtagcgc cagtccgagc ccgaggtacg ccccgagcag
     4501 cgggttcggc cgttcacgtg ggagatacgg agtcgtccgc ggcacgccga tgcagatggc
     4561 gtcgagttgg ccgtcgagcg tgaggcctcg tagctcgacg ccgcgcagca gcgcctccga
     4621 gtgtgcgacg gacggcgggc ctgcgaaggc ggcggcgacc gtgcgctcta gaggcaccga
     4681 acgcaagacg tacgcgcgca gcgcttcggg caacgcgcgg aagatatgac ggaacggtga
     4741 gcgcacgacg cgctccagcg cctgctcctc gtaggggtag ccgccgaggg tgccgccgaa
     4801 gcgcggatgg ttgagaacca gtgaggcgcc gatcaccgga acgcggcgcg cgagcgcgcg
     4861 ctcgagctcg agcccgagcc gccatccttg cgacgccgag gtctcgagca gagagtaggc
     4921 cccggccgcg cgcagcacct cggtccccgc ggcgccgacg aacgcggcgg gtcctccgtg
     4981 cagcaccgtc tcggccgcgg tcacgacgac gacgacgtcg gtgtgcacca gcgcagggtg
     5041 gacgcgcaac gggatcgagc cggcggtgcc gagctcgacg aggtcgtcgc gctcgacgtc
     5101 gtgcacctcg acatgaccgt gaaagcgtcg cgcgaactca ggggagacga gcgtctcgag
     5161 ctgctcgttt ccggctcggc gcgtgagacc accggcgacg aggagtgtct gatatccggt
     5221 cgggatgccc aggcgctcca gctctgcgac ggccgctgcg atcgcagcct gacgcgggtc
     5281 gcgctccgca cccggcagcg gtaggtgcgg cggctcgacg acgattgtcg cgcgcgatcc
     5341 gcgcgaaacg agcgcttcga gcggctcccc ggcgagcggg aagcgcaacg cgtcgcggac
     5401 ggccgccccc acgtccgcga cggagtccgc cggtagaggc ggtcgcagga cgatcgcatc
     5461 gtcgtcagcg gtcacgacta cgactcgcga cccggacagg agtgggatcc gcctcaccgc
     5521 agagatcgac tcagatcagg ctcggaaaat gggtgaatcg ggagcgctcg catcggcggc
     5581 ggacgatagc gttgaggccc atgcccaaga ccctgacagg cgccgagctc cagctggacg
     5641 acagccctgt ccgtgatgcc gagctcttcc tcgtagacgg caacaacctc gcctaccgcg
     5701 ccttcttcgc actgcccgaa gagctggcga cgaccgaggg ctttcccacg aacgcgctcc
     5761 tcgggttcac gaacatgctg ttcaagctgc tctcggacta caagccgaaa ggcgtcgccg
     5821 tggcctggga ctcgagc
//
LOCUS       DASVEU010000204        10821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1500181,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000204 DASVEU010000000
VERSION     DASVEU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500181"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VF225_08525"
     CDS             <1..989
                     /locus_tag="VF225_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX7145339.1"
                     /translation="LPKDYVRLRLTGGEHAIDVADASGTLLFDVAGRRWSREMLEGLE
                     LDPAWLPRALESPEVSGETATGIPVAAGAGDQAAAALGVGVDRPGPVSVVLGTSGTVF
                     AALPAFAADPQARVHAFCHAVPGGWHAMGVMLSAAGSLRWLRDALGGGSGYDELTAAA
                     QEWPPATDGLTFLPYLTGERTPHADPHARAAFAGLTLRHDRGALVRAVLEGVAYGLRD
                     SLELLRELGVDPRAGHVSGGGARSELWLRIIASVLGLPMQRAANDEGSAYGAALLGGV
                     AAGVFRDVHEAVATCVRLLDPIEPDPQWGAAYERGYRRFRLLYPALRPLEDA"
     gene            986..1969
                     /locus_tag="VF225_08530"
     CDS             986..1969
                     /locus_tag="VF225_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7145340.1"
                     /translation="MTTLDAAVKWGILSTADINRKIIPGAHASEKVELIAVASRDLRR
                     AEEYAREWEIDRAYGSYEALLEDADVEAVYISLPNTLHCDWSIRAVEAGKHVLCEKPM
                     SRHVGDVEAAFDAAEKAQRILMEAFMYRHNPQTKRLRQLVDEGAIGDLRLVRSCFSYS
                     LYDVENIRLRPEVEGGSLMDVGCYCVSGSRLLAGEPEQVYGEQFLGESGTDWVFTGTI
                     RFPGDVFAQFDCGTALPERDELEAIGSDGSLFLDDPWHCIEPAIELRRDGGMERIEVE
                     PANSYRLELENVSEAIRGVADPLLGREDAVGQARAIDALYRSAVSGTAASL"
     gene            complement(1930..3087)
                     /locus_tag="VF225_08535"
     CDS             complement(1930..3087)
                     /locus_tag="VF225_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145341.1"
                     /translation="MRRLLLLTGAIVLVDTMFFSALTPLLPHYVDDLHLGKAGAGVLQ
                     AMYPAGALVAGIPGGMAAARLGVKPTALIGLSLLAVTTVAFGLADSVWLLDLARFLQG
                     ISSAFSWTGALTWLVAAAPPGRRGQLIGSAFGAAVVGALFGPVLGAAASFTGTGPAFG
                     AVALLAVVLAGAAAATPAATPESPQPLRTLVDAVLRNRTIQTGVWLCALPALCFGTLS
                     VLAPLRLSDLGWGAAAVGATFLVSAGLQAVWAPVLGRVSDQHGRLPPLFGSLAASALV
                     ALLLPWPRQALLLAFLVVCAGSAFASFWPPAMSLVTDAAEQSGLEYGYAFALINVAWA
                     PGQAGGSAIGGAVASATTDAVSYLGLTVLCLVTLAALLRYKEAAVPLTAER"
     gene            3282..4649
                     /locus_tag="VF225_08540"
     CDS             3282..4649
                     /locus_tag="VF225_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7145342.1"
                     /translation="MSATASIERDLGAAVAADVVAPTAAYLTDMTEARGLIGHADAVA
                     LPRTAEQVAEVVAWCYAHGVAIVPRGGGTGYAAGAVPLDGGVVLALERLDRVRSFDPL
                     LWRIEVEAGLRTAELRRIAREGGLLFAPDPGAAEQSQIGGNIATNAGGPHAFKYGVTG
                     KWVTGLEVVVPPGELVRLGGPLRKDVAGYDLKHLLIGSEGTLGVIVSAWLQLTPAPEA
                     AWPVIGFFDGVPGGVGAIERVIGTGLPVAALEYIDSETMRYASASFPGEVPDGGFAVL
                     AEADGSTEEAARIRKDVLDVFGEDALALYAPEAPQEIAALWRWRDGLALVVDAQRGGK
                     ASEDIAVPLDRLGEAIEESLEVGRRLGVPTCSWGHAGDGNLHTVFLLAGDNEAELALA
                     PKLSEGLFELALRLGGTISGEHGVGFVKRNWLAKQLGPRAFELHTAVKRAFDPQNLLN
                     PGKKA"
     gene            4646..6265
                     /locus_tag="VF225_08545"
     CDS             4646..6265
                     /locus_tag="VF225_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7145343.1"
                     /translation="MIQKLLVANRGEIALRVFRTARDLGIATVAVVAPDDKGSLHARS
                     ADETIDVASYLFSEEHIRAAKQSGADAIHPGYGFLAENPDFAEAVNAAELTWVGPPPE
                     ALRAGGDKLEAKRIASEAGVPVVPTGEPDEIGFPLVVKAAAGGGGRGMRVVREPSELE
                     EALAAARREAKAAFGDERVFCERYVERPRHVEVQLFGDAQGNVTSLFERECSIQRRHQ
                     KVLEESPSPGIDPELRLRIIMSALAFAEAVGYQNAGTAEFMVHGGDYYFLELNGRIQV
                     EHPVTELVTGADLVRWQIEVAQGTRTVDHYLPESRGHAVEVRLYAEDPRTFFPQTGRI
                     EGLTLPEGIRVDAGVAEGDEIGIAYDPMIAKLIAHGETRDESFDRLRDALQETAIEGV
                     TTNLPFLRWLVSHPVVRAGDTTTAFLTEHPPLSPAPARVAAAPWRTPFRLNLPSSAST
                     APPDVDALAADHRGPAGGQSSVTAPMPGTVIRLLVSTGDSVRARQPLVVLEAMKMETP
                     LTSPYDAVVRAVHVAEGDRVAGGTVLVELEE"
     gene            complement(6266..6835)
                     /locus_tag="VF225_08550"
     CDS             complement(6266..6835)
                     /locus_tag="VF225_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX7145344.1"
                     /translation="MTWYTFFKSVHVITAAIWVGGAAMIQAYAFRILRTGDGRRQAEF
                     SKDTEVVGMRVFMPSTLILFLAAIAMMVNLDWSWSQNWIILGLIAFGLSFVLGAGFLG
                     PEGGRIAELIERDGPDSPAVQARIRRILTISRCELVVLLTVMLNMVVKPTGNNGWFWG
                     MLVAMVLGIATVLALSGRAADQVPAPATE"
     gene            complement(6845..7150)
                     /locus_tag="VF225_08555"
     CDS             complement(6845..7150)
                     /locus_tag="VF225_08555"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX7145345.1"
                     /translation="MTGVKEFFDGLESRADASKTAGMTNSYLFDIDGAGKWTVKVEDG
                     KVSVNEGGEDADAVITTSEETFQKIISGEQNPTSAYMTGKLKVKGDMGAAMKLQKLF"
     gene            complement(7167..7442)
                     /locus_tag="VF225_08560"
     CDS             complement(7167..7442)
                     /locus_tag="VF225_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145346.1"
                     /translation="MGLTRFNFDRRMPMMTLEMSATLRRPSVRSRFRRGHSRPTVEGL
                     LEEIARLTAERQNLREQGVNGSRLERNRVKLARKQWELSHALIDRYL"
     gene            7492..8994
                     /locus_tag="VF225_08565"
     CDS             7492..8994
                     /locus_tag="VF225_08565"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7145347.1"
                     /translation="MGEVTIEVKAPGDGQADSLAIPYSAGSPAGAFSNGARELDGQLK
                     GRLQRLADGGELKSDLGATVVLLTDGEVKARRIVVAGVGKRDEIDADALRTAASAVAH
                     SVADVGGAVGWLLDESLPLSLEEQARAIVEGTMLGSYSPARWKTQEQPEAGVDKIVLY
                     TSAGNGLAQTASRSASVGRWVNVARDLANSPPNELTPETLAARAVELAGPKLIVEALD
                     PGRIDELGMGALSAVGRASSNGPRLIVLRYEPAQATRKDLVLGLVGKAITFDAGGISL
                     KPALKMQDMKGDMAGGAAVIAAMGAVAELELPVRIVAVVAAAENLVSGDSFRPGDILR
                     AANGKTIEITNTDAEGRLVLADALWYARREGATHLLDLATLTGAMELALGDFYAGAFA
                     NDDDWMAEILAAAEASGDHAWPFPLHPRYRRYVDSSFADLKNSSDLRQGSPVLAAEFL
                     REFSGEGPWAHIDMAGPGFLERSRGDYLTQRGGTGYGVRLIVELAQRLSS"
     gene            8991..10124
                     /locus_tag="VF225_08570"
     CDS             8991..10124
                     /locus_tag="VF225_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145348.1"
                     /translation="MNFDLDQEHELVRSTVREFAEKRVAPVAEELDRESRFPYELVGE
                     LAELGLMGMTIPEEYGGAGADTVSYAIAVEELTRVDSSVAITVAAHHSLGTLPIFYFG
                     SEEQKQQWLPDLSSGKKLAAFGLTEPDAGSDAGATRTHAELRDGEWVVNGSKIFITNA
                     GTDITACVTITARTGDDEISNVIVPNGTPGYEISKPMKKLGWRASDTRELSFKDVAVP
                     EGNLLGPRGQGFQQFLEILDGGRISVAAMGVGLAQGAYDLAFAYAQERRQFGKPIAKF
                     QAVQFKLADMATELEAGRNLVYKAAWLKDEGRDFALAAAQAKLYTGELSNRAVNWALQ
                     IHGGYGYMDEYPISRLYRDQKILEIGEGTNEVQRMVIARHLGV"
     gene            complement(10261..>10821)
                     /locus_tag="VF225_08575"
     CDS             complement(10261..>10821)
                     /locus_tag="VF225_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145349.1"
                     /translation="YRASLSSAGAVDVSVGGRVVVFQVPAKTRPAGAIVARATEAFRR
                     LRSVGYVERLASDPRNKVVAEFTLERPNRLEYRIRGGADGIIIGPRRWDRQRGGKWVP
                     SPQDVTPQPEPIWAGHSTNAYLLESTPTTYVVSFFKPIGPVWFTLRLDRRTLLPRSLR
                     MTAAAHFMTHRYTTFNAPRKIRAPSR"
BASE COUNT         1777 a         3612 c         3783 g         1649 t
ORIGIN      
        1 tgctcccaaa ggactacgtc cgactgcgtc ttacgggcgg cgagcacgcg atcgacgtcg
       61 cggacgcgtc gggcaccctg ctgttcgacg tcgcggggcg gcgctggagc cgggagatgc
      121 tcgagggact cgagctcgac ccggcctggc tgccgcgagc gctggagtcg cctgaggtct
      181 caggcgagac ggccaccggc attccggtcg ccgcgggtgc cggcgatcag gcggcggctg
      241 cactcggtgt cggcgtcgac cggccgggac ccgtctcggt ggtgctcggt acgtcaggca
      301 ccgtgttcgc ggcattgccg gctttcgccg ccgatccgca ggcgcgcgtg cacgcgttct
      361 gccatgccgt tccgggcggc tggcacgcga tgggcgtcat gctgtccgca gcaggctccc
      421 tgcgctggtt gcgtgatgct ctcgggggcg ggagcgggta tgacgagctg accgccgcgg
      481 cccaggaatg gccccccgcc accgacgggc tgaccttcct cccctacctc accggtgagc
      541 ggacgcccca cgcggacccg cacgcccggg cggctttcgc agggctcacg ctccgccacg
      601 accgcggcgc gctcgtccgc gccgtcctgg agggtgtcgc ctacgggctg cgcgactcgc
      661 tcgagttgct gcgcgaactc ggggtcgacc cgcgtgccgg ccacgtttcc ggcggcggag
      721 cccgtagcga gctctggctt cgcatcatcg cctcggtcct cggcctgcca atgcagcgcg
      781 cggcgaacga cgagggctcc gcctatggag cggcgctact cggcggcgtg gccgcaggag
      841 tctttcgcga cgtccacgag gccgtcgcca cctgtgttcg tctgctcgac cccatcgagc
      901 cggaccctca gtggggtgcc gcctatgagc gcggttaccg ccgcttccga ctgctctatc
      961 cggcccttcg acccctggag gacgcatgac gacgctcgac gcagcggtca agtgggggat
     1021 cctttcgacc gccgacatca accggaagat catccccggc gcgcacgcct cggagaaggt
     1081 cgagctcata gccgtggcga gccgcgatct gcgacgggcg gaggagtacg cgcgcgaatg
     1141 ggagatcgac cgtgcgtacg gctcgtacga ggcgttgctc gaggacgccg atgtcgaggc
     1201 cgtgtacatc tcgctgccga acacgctgca ctgcgattgg tcgatccgcg cggtcgaggc
     1261 gggaaagcac gtgctctgcg agaagccgat gagcaggcac gtcggcgacg tcgaggctgc
     1321 attcgacgcg gcagagaagg cgcaacgaat cctgatggag gccttcatgt accgccacaa
     1381 ccctcagacc aaacggctcc gtcagctcgt ggacgagggc gcgatcgggg acttgcggct
     1441 cgtccgctcc tgcttcagct attcgctgta cgacgtggag aacatccgcc tgcggccgga
     1501 ggttgagggc ggcagcctga tggatgtcgg gtgttactgc gtgagcggat cccgtttgct
     1561 cgccggtgag cccgagcagg tgtacgggga gcagttcctc ggcgagagcg gcaccgactg
     1621 ggtcttcaca ggcacgatcc gcttcccggg cgacgtcttc gcccagttcg actgcgggac
     1681 agccctgccc gagcgcgacg agctcgaggc gatcgggagc gacggctcgc tcttcctcga
     1741 cgatccatgg cactgcatcg agccggcgat cgagcttcgc cgcgacgggg ggatggagcg
     1801 gatcgaggtc gagcccgcca actcgtaccg gctcgagctc gagaacgtca gcgaggccat
     1861 ccggggcgtc gccgatccac tcctggggcg cgaggacgcc gttggtcagg cccgcgcaat
     1921 cgacgcgctc taccgctcgg cggtaagcgg cacggcggct tccttgtagc gcagcagtgc
     1981 ggcaagcgtc acgaggcaga gcacggtgag gccgaggtag gagaccgcgt cggtggtcgc
     2041 ggatgcgacc gcgcccccga tcgccgaacc gccggcctga cccggcgccc aggcgacgtt
     2101 gatcaacgcg aatgcgtagc cgtactcgag cccgctctgc tcggcggcgt ccgtcaccaa
     2161 cgacatggcc ggcggccaga agctcgcgaa ggccgagccg gcgcagacga cgaggaacgc
     2221 cagcagtagg gcttggcgcg gccagggcag cagtagggcg acaagcgccg aggcggccag
     2281 ggagccgaag agcggcggca ggcggccgtg ctggtcggaa acccggccca gcaccggcgc
     2341 ccagacggct tggagtcccg cggagacgag gaaggtcgcc ccgactgccg ccgcgcccca
     2401 gccgagatcc gaaaggcgca agggggcgag gaccgacagc gtcccgaagc agagagcggg
     2461 aagagcgcag agccacactc cagtctggat cgtgcggttg cgcaggacgg cgtccacgag
     2521 cgtccggagc ggctgcgggc tttcaggggt cgcggcgggc gttgccgcgg cggcgccggc
     2581 gaggacgacc gcgagcagtg caaccgcccc gaacgccgga ccggtgccgg tgaaggacgc
     2641 ggctgccccg agcacggggc cgaagagcgc tcccacgact gccgcgccga acgccgaccc
     2701 gatcagttgg ccgcgccggc cgggcggcgc ggcagcgacg agccaggtca gggcgccggt
     2761 ccaagagaac gcactcgaga ttccctgcag gaagcgcgcc aggtctagca gccagaccga
     2821 atccgccaac ccgaaggcaa ccgtcgtcac tgcgagcagt gacagcccga tcaacgcggt
     2881 gggcttgacg ccgagacgcg cggcggccat cccgcccggg attccggcca cgagtgcacc
     2941 ggcggggtac atggcctgca acacgcccgc accggccttg ccgaggtgga ggtcgtcgac
     3001 gtaatgcggg aggagcggcg tcagcgccga gaagaacatg gtgtcgacga ggacgatcgc
     3061 cccggtcagt agcaacagcc tgcgcacagg ccgaggctaa ccgcaggagt gtgtttcagg
     3121 aggaagcctc agcgaactgg cgacagaggt gcatcatcta cgctccgttt cgtggacttg
     3181 caacaggtcg cacggggttt gtggcgctgg gtactgacac accccgagtg ggagccgccg
     3241 cagcaggaag acagccgggc tcatcggccg gcgtgcggcc gatgagcgcg actgcatcca
     3301 tcgagcgtga cctcggcgcg gcagtcgccg cggacgtcgt cgcaccgacc gccgcctacc
     3361 tgaccgacat gaccgaggca cgcggactga tcggtcacgc ggatgccgtg gcgctcccgc
     3421 ggacagcaga gcaagtggcc gaggtcgttg cttggtgtta cgcgcacggc gtggcgatcg
     3481 tgccgcgcgg cggcgggacg ggttacgccg ctggtgccgt cccgctcgac ggcggcgtcg
     3541 ttctcgcact cgaacgtctc gaccgcgtgc gttcgttcga cccgctcctt tggcgcatcg
     3601 aggtcgaggc tgggcttcgc actgcggagc tgcggcggat cgcacgcgag ggcggcctgc
     3661 tgttcgctcc cgatccgggc gcggccgagc agtcgcagat cggcggcaac atcgccacga
     3721 acgcgggcgg gccgcacgcg ttcaagtatg gcgttaccgg caaatgggtg acaggcctcg
     3781 aagtcgtcgt tccgcccggc gagctcgtgc ggctcggcgg ccctttacgc aaggacgtcg
     3841 ccggatacga cctcaaacat ctgctgatcg gatctgaagg cacgctcggc gtgatcgtct
     3901 ccgcgtggct gcaactcacg cccgctccgg aggccgcgtg gcccgtgatc ggattcttcg
     3961 acggcgtccc tgggggcgtc ggggcgatcg agcgggtgat cggcaccggt ctgcccgtcg
     4021 ccgcactgga gtacatcgac tcggaaacga tgcgatacgc gagtgcgagc tttccgggcg
     4081 aggtgcccga cggtggcttc gccgtgcttg cagaggcgga cggctcgacc gaggaggcgg
     4141 cgcggatccg caaggacgtg ctcgacgtgt tcggcgagga cgctctagcc ctctacgccc
     4201 ccgaggcgcc acaggagatc gccgccctct ggcgctggcg tgacggtctg gcgctcgtcg
     4261 tggatgccca gcgcggcggg aaagcaagcg aggacattgc agtgccgctt gaccggctcg
     4321 gcgaagcgat cgaggagtcg ctcgaggttg gacggcggct tggcgtgccg acgtgctcgt
     4381 gggggcacgc aggcgacggc aacctgcata ccgtgttcct gctcgccggg gacaacgagg
     4441 cagagctcgc actcgcgcca aaactctccg aaggcctctt cgagctcgcc ctgcggctgg
     4501 gtggaacgat ttccggtgag cacggcgtcg gctttgtgaa gcggaactgg ctcgcgaaac
     4561 agctcggtcc tcgtgcgttc gagttgcaca ccgccgtcaa gcgcgcgttc gacccgcaga
     4621 acctgctcaa cccgggaaag aaggcttgat ccaaaagcta ctcgtcgcga atcgcggcga
     4681 gatcgcgttg cgcgtcttcc gcaccgcacg cgacctgggg atcgccacag tggccgtagt
     4741 cgcccccgac gacaaaggct cgctccacgc ccgctctgcc gacgagacga ttgacgtcgc
     4801 gagctacctc ttctccgagg agcacatccg cgcagcgaag cagagtggag cggacgcgat
     4861 tcatccgggc tacggctttc tcgccgagaa ccccgacttc gccgaggcag tgaacgccgc
     4921 cgagctgacc tgggtcggcc caccacccga ggcgttacgg gccggcggcg acaagctcga
     4981 ggcaaagcgg atcgcgagcg aggcgggcgt tccggtcgtc ccgaccggtg agcccgacga
     5041 gatcggcttt ccgctcgtcg tcaaggcggc tgccggcggc ggcggacgcg ggatgcgcgt
     5101 cgtccgtgaa ccttcagagc tggaggaggc gctcgcggcg gcgcgacgag aggcgaaggc
     5161 cgccttcggc gacgagcgtg tgttctgcga gcgttatgtc gagcggccgc ggcatgtcga
     5221 ggttcagctc ttcggcgatg cgcaggggaa cgtgacctct ctcttcgagc gcgagtgctc
     5281 gattcagcga cgtcaccaga aggtcctcga ggaatcgccg tcgcccggca tcgatcccga
     5341 gctccgcttg cggatcatca tgtccgcgct tgcgtttgct gaggcagtgg gctaccagaa
     5401 cgccggcact gcagagttca tggtccacgg aggcgactac tacttcctcg agctgaacgg
     5461 ccgcatccag gtcgagcacc cggtcaccga gctcgtgacg ggcgcggatc tggtccgctg
     5521 gcagatcgag gtcgcgcaag ggacgaggac ggtcgaccac tatctgccgg agagtcgcgg
     5581 tcacgcagtc gaggtgcgac tctacgccga ggacccgcgg acgttcttcc cgcagaccgg
     5641 ccgcatcgaa gggttaacgc tgcctgaagg aatccgcgtc gacgcgggcg tcgccgaggg
     5701 cgacgaaatc ggaatcgcct acgatccgat gatcgcgaaa ctcatcgcgc acggagaaac
     5761 gcgagacgag tcattcgacc gtcttcgcga tgcgctgcag gaaaccgcga tcgaaggcgt
     5821 cacgacgaac ctgccgtttc tacgctggct cgtttcgcat cccgtcgtcc gcgcgggcga
     5881 cacgacgaca gcattcctca ccgagcaccc gccgctctcc cccgctcccg cccgagtggc
     5941 cgccgcgccg tggcgcactc cgttccgcct gaaccttccg tcatcggcct cgacggcgcc
     6001 gccagacgtc gacgccctcg cagcggatca ccggggcccg gccggcgggc agagcagcgt
     6061 caccgccccg atgcccggaa cggtgatccg gctcctcgtc agcacaggcg actcggttcg
     6121 agcccgccag ccgctcgtcg tgctggaagc gatgaagatg gagacgccgc tgacgtctcc
     6181 ctacgacgcc gtcgtgcgtg cagtgcatgt tgcggaaggc gaccgggtcg ccggtggcac
     6241 cgtcctcgtc gagctcgagg agtagttact ccgtcgccgg agccggaacc tggtccgcgg
     6301 ctcgcccgga caaggccagg acggtggcga tgccgagcac catcgccacg agcattcccc
     6361 agaaccagcc gttgttgcct gtgggcttca cgaccatgtt cagcatcacg gtcagtaaca
     6421 cgacgagctc acagcgcgag atcgtgagaa tccgccggat gcgagcctga acggcgggtg
     6481 agtctggtcc gtcccgctcg atgagctcgg cgatccggcc accttccggg ccgagaaatc
     6541 ccgcgccgag gacgaacgag agcccgaagg cgatcaggcc gagaatgatc cagttctggc
     6601 tccacgacca atcgaggttc accatcatcg cgatcgcggc gaggaacagg atcaaggtgg
     6661 acggcatgaa cacccgcatg ccgacgacct ccgtgtcctt cgagaattcc gcctgccgcc
     6721 taccgtctcc cgttcggagg atccggaagg cataggcctg gatcatcgcc gctccgccga
     6781 cccagatcgc agccgtgatg acgtggacgg acttgaagaa ggtgtaccag gtcatgtagg
     6841 cgcgctagaa gagcttctgc aacttcatcg cggcacccat gtcgcctttg accttcagct
     6901 tgccggtcat gtaggcgctg gtcggattct gctcgccgct gatgatcttc tggaacgtct
     6961 cctcggaggt ggtaatgacg gcgtcggcgt cctccccgcc ttcgttgacg ctcaccttgc
     7021 catcctcgac cttgacggtc cacttcccgg cgccgtcgat gtcgaagagg tacgagttgg
     7081 tcatccccgc cgtcttcgac gcgtcggcac ggctctcgag gccgtcgaag aactccttca
     7141 ctcccgtcac gggagcaacc ctacactcag aggtagcggt cgatcagcgc gtgcgagagc
     7201 tcccattgct tccgggcgag cttgacgcgg ttgcgctcga gcctggatcc gttgacgccc
     7261 tgctcgcgca ggttctggcg ttcggcggtg agcctggcga tctcctccag cagcccctcg
     7321 accgtcgggc gcgagtgccc gcgccgaaag cgcgagcgga ctgagggccg ccgcagggtt
     7381 gcgctcatct cgagggtcat catcggcatg cgacggtcaa agttgaaccg ggttagcccc
     7441 atttcgtaag gagacggtaa aaccctgcac ctcggatacg atcgcgaccc gatgggggag
     7501 gtcacgatcg aagtcaaggc gcccggcgat ggccaagccg actcccttgc gatcccctat
     7561 tccgcgggaa gccctgcggg tgcattctcg aacggcgctc gcgagctgga tgggcagctc
     7621 aagggccggt tgcagcgcct tgccgacggc ggcgagctga agagcgatct cggggcgacc
     7681 gtcgtcctgc ttacggacgg cgaggtgaaa gcgcggcgca tcgtcgtcgc cggggttggg
     7741 aagcgggacg agatcgacgc ggacgcgttg cgcacggcag cctccgcggt cgcgcacagc
     7801 gtcgcagatg ttggcggggc ggtgggctgg ctcttggacg agtcgctccc cctctccctc
     7861 gaggaacagg cccgggcgat cgtcgaaggc acgatgctcg ggtcgtacag ccccgcccgc
     7921 tggaagacgc aggaacagcc cgaggcaggt gtcgacaaga tcgtgctcta cacctcggcc
     7981 ggcaacggcc tggcgcagac cgcgagccgg agcgccagcg tcggcaggtg ggtcaacgtc
     8041 gcccgcgatc tcgccaactc gccgcccaat gagctgacac ccgagacgct cgccgcgcgc
     8101 gcggttgagc tcgcagggcc caagctcatc gtcgaagctc tggatcccgg gcggatcgac
     8161 gagctcggca tgggggcgct ctcggctgtc ggccgcgcga gctcgaacgg gccgcgcctc
     8221 atcgtccttc gttacgaacc cgcgcaagcg acgcgcaagg acctcgtgct cggcctcgtc
     8281 ggcaaggcca tcaccttcga cgcaggcggc atctcgctga agcccgcgct gaagatgcag
     8341 gacatgaagg gcgacatggc cggcggcgcg gccgtgatcg cagcgatggg ggccgtcgcc
     8401 gagctggagc tgccggtgcg aatcgtcgcc gtcgtcgccg cagcggagaa tctcgtcagc
     8461 ggcgactctt tccggccggg cgacatcctt cgcgctgcga acggcaagac gatcgagatc
     8521 acgaacaccg acgccgaagg acgcctcgtg ctggccgatg cgttgtggta cgcacgccgc
     8581 gagggcgcga cacacctgct cgacctcgcg accctgactg gggcgatgga gctcgcgctc
     8641 ggggacttct acgcgggcgc gttcgcgaat gacgacgatt ggatggccga gatcctcgcc
     8701 gctgcagaag cgagcggcga tcacgcctgg ccctttcccc tccatccgcg ctatcgccgc
     8761 tacgtcgact cttccttcgc cgatctgaag aactcctccg acctccggca gggctcgccg
     8821 gtcctggcgg cggagttcct gcgcgagttc tccggcgaag ggccgtgggc gcacatcgac
     8881 atggctgggc ccgggttcct ggaacggtcg cgcggcgact acctcacgca gcgcggcggg
     8941 accggctacg gcgttcgact gatcgtcgaa cttgcgcagc gcctctccag ttgaatttcg
     9001 acctcgacca ggagcacgag ctcgtccgct cgaccgtgcg cgagtttgct gagaagcgag
     9061 tcgcgccggt ggcggaggag ctcgaccgcg agagtcgctt cccgtacgag ctggtcggcg
     9121 agctcgctga gctcgggctc atgggcatga cgattccgga ggagtacggc ggcgccggcg
     9181 cggacaccgt ctcgtacgcg atcgcggtcg aggagctcac gcgcgtggat tcgtcggtcg
     9241 cgatcactgt cgcggcccac cattcgcttg ggacgctgcc gatcttctac ttcggcagcg
     9301 aggagcagaa gcagcagtgg cttcccgacc tttcgtcggg aaagaagctc gccgcattcg
     9361 gtttgacgga gccggatgcg ggctccgatg cgggcgcgac tcgcacgcac gcggagttgc
     9421 gcgacggtga gtgggtcgtg aacggctcca agatcttcat cacgaatgca ggcaccgaca
     9481 tcacggcgtg cgtgacgatc accgcccgta caggcgacga cgagatctcc aacgtcatcg
     9541 tgccgaacgg gacgcccgga tacgagatct cgaagccgat gaagaagctc ggttggcgcg
     9601 cgtcggacac gcgcgagctc tccttcaagg acgtggcggt ccccgagggg aacctcctcg
     9661 gcccgcgggg tcaaggcttc cagcagttcc tcgagatcct cgacggcggg cggatctcgg
     9721 tcgccgcgat gggcgttggc ctcgcgcagg gcgcgtacga cctcgcgttc gcctacgcgc
     9781 aggagcggcg gcagttcggc aagccgatcg cgaagttcca ggcggtgcag ttcaagctcg
     9841 cggatatggc cacggagctc gaggccggcc ggaatctcgt ctacaaggcc gcctggctca
     9901 aggacgaggg ccgcgatttc gcgctggcgg cggcgcaggc gaagctctac accggcgagc
     9961 tctcgaaccg cgccgtgaac tgggctctcc agatccacgg cggctacggg tacatggacg
    10021 agtacccgat ctcgcgcctc tatcgggacc agaagatcct ggagatcggc gagggcacga
    10081 atgaagttca gcgaatggtg atcgcgcggc acctcggcgt ctaagacggc acgcaggcgt
    10141 cacgctttcg gagccgtgcc tggcaccggt acgaccaccg ggagccgcgg cgccgcgcgg
    10201 gctcgctgag ttctgcgagg aactcgttac acgggcgtac ccgtgccagg caccggtacg
    10261 tcagcgagaa ggtgcgcgga tctttcgagg tgcgttgaag gtcgtgtagc gatgcgtcat
    10321 gaaatgcgcc gccgctgtca tgcggaggct gcgcggcagc aacgtcctcc ggtcgagacg
    10381 cagcgtgaac cacacggggc cgatcggctt gaagaacgag acgacgtacg tcgtcggggt
    10441 cgactcgagc agatacgcgt tcgtcgagtg gccggcccag atcggttccg gctgcggcgt
    10501 cacgtcctgg ggcgacggca cccacttgcc ccctcgctgg cgatcccagc gccgcgggcc
    10561 gatgatgatc ccgtccgcgc cgccgcggat tcggtactcc agccggttgg ggcgctccag
    10621 cgtgaactcg gcgaccacct tgttccgggg gtcggaagca agccgctcga cgtagcccac
    10681 gctgcgcagg cggcggaacg cctcggtggc gcgcgcaacg atcgcgcccg caggacgggt
    10741 cttggccggt acctggaaga ccaccacgcg accgccgacg gagacgtcga cggcgccggc
    10801 cgaggacaac gaggcccggt a
//
LOCUS       DASVEU010000205        16991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_338105,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000205 DASVEU010000000
VERSION     DASVEU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16991
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338105"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF225_08580"
     CDS             <1..411
                     /locus_tag="VF225_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7145350.1"
                     /translation="DLAMPRRVATGVDPKRLAMIVAVLGRHARIALGSADVFVNVAGG
                     VRIDEPGADLAVALAIASAAKGVPTKEALAAFGEIGLTGRLRPAAQAERRLEECAKLG
                     LKTVVAPADTPATRRKLEVLQAETLPKAITAGLS"
     gene            477..1604
                     /gene="disA"
                     /locus_tag="VF225_08585"
     CDS             477..1604
                     /gene="disA"
                     /locus_tag="VF225_08585"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX7145351.1"
                     /translation="MQEAQAALGFTRTPSEDDPRRDPELLAALRRIAPGTDLRQGMDD
                     IIRSREGALIVIGDPNELSFLFSGGIRLDVPFTGQLLYELAKMDGAIIVNPSRSKLAY
                     ANVQLMPDPTIPSVETGTRHRTAERVAKQTGALVISISQQRSTISVYVGPTRYQLDAV
                     PEVLGKTNQALATLETYGQRLQQVLTRLTALEFQNAVVLDDVLVVLQRAEMTTRMAAE
                     IERDCVELGAEGRLIRMQLNELVGEVPAERALVVHDYHADGSGPRAELALEGLHALPY
                     RELLESERLAELLGFPREVNPLDHSVVPRGLRILSRIPRLPDAVVKKVVGDLGDLDSI
                     VRASQRDLERIGGVGAVRAREIREGLRRLQEHNLVERYLHL"
     gene            1601..2722
                     /locus_tag="VF225_08590"
     CDS             1601..2722
                     /locus_tag="VF225_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145352.1"
                     /translation="MSRATGLAAGYAVFGLFWGSWAACLPAIQRETGASDTQLGLALL
                     CVALAALPAMLVAGRLADVFGARLVPIFLVFFGAVEWLPGLAHSVPMLVLLLAVIGVG
                     TGLLDIAINAHAARLEALHGIRIMDGLHAAFSGGVLVGGIGSGLLRHAGAHPVWILAG
                     VGLVTALTALANLGPYPPLAPRQRRARLARPLLIVGLVLALAFLVENGLEAWSALFLE
                     RTLNSSAAVSGLGPGLFAGAMLTGRVLAQRVEWPSVAGRMLFAGLAAAAGLALAATAQ
                     HAAVALAGFVVAGGGLALSAPTLFGLAGRVGGEGGRGAALSTVAILGYLGFVAGPALI
                     GAVSGASSLRGGLLFLCAVAVILAACAPILRRVVREIGG"
     gene            2837..3469
                     /locus_tag="VF225_08595"
     CDS             2837..3469
                     /locus_tag="VF225_08595"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7145353.1"
                     /translation="MYKIGDKVVYPHHGAGTVVKREKREVLGEKREYLTIKILHNDMT
                     VNVPSENAEAVGLRKVIGEDMVKVVVKALTGGGTQMPKNWNRRFKHNRDKMKTGDILE
                     LAEVVRNLSLRDSEKGLSTGEKQMFVKAKKILASELMYAKDMDEEEAAEWLDGVLVGS
                     ANGSTNGRKKTTAAKPAKVASKTSAKPAARKTAATAAKAKPKTKARASSR"
     gene            3466..4137
                     /gene="ispD"
                     /locus_tag="VF225_08600"
     CDS             3466..4137
                     /gene="ispD"
                     /locus_tag="VF225_08600"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX7145354.1"
                     /translation="MSVWAVLAAAGSGDRLGGDRPKAFARLGDEPLLAQSLKRLDDSD
                     WVDFIVVAAPPGWEEPAILLAEELGCSKVSACVTGGATRGQSVARALAEVPEDAAVVL
                     VHDAARPLVSDEVIERVLTALNEGWDGALPGLQLADTIKHVDEEGRIVETLNRDELRA
                     VQTPQAFVASVLRDAYAKGDTAVATDCSSLVEARGGRVKVVEGDPKLIKVTDAADLGR
                     IAALL"
     gene            4134..4937
                     /locus_tag="VF225_08605"
     CDS             4134..4937
                     /locus_tag="VF225_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX7145355.1"
                     /translation="MIVDYHMHLRNGSGEIAHETSAIDPFVESAREAGVDEIGFTEHI
                     YYFKQTRSLWTVPYHTERCVYDLEAYVSAVVTARGRGLPVKLGLEVDYVPGREDETRE
                     LLAPYPWDYLLGSLHYIGSLGVDDEPRLVDAVGVEDAWRLYFETLAAAARSGLFDSLS
                     HPDLIKIFGARAASFDYEPVVAAIADSGVAVEVSTAGLHKPVHELYPHPEFLAACRAL
                     DIPVTLASDAHTPDVVGRDFDRALELLRSAGYDTVTVFEQRRARQEPLG"
     gene            4934..5404
                     /gene="ispF"
                     /locus_tag="VF225_08610"
     CDS             4934..5404
                     /gene="ispF"
                     /locus_tag="VF225_08610"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7145356.1"
                     /translation="MSLRVGIGVDAHQFEDGMPLVLGGVGFESSRGLAGHSDGDVITH
                     ALIDAVLGAAGLGDIGSLFPSDSSTPAGISSLDLLRDAVARVRDAGYAVVNADCVLIG
                     EEPRISGRRTEMADALSAVVGAPVNVRATTTDGLGFTGRGEGLGAQAVALVESP"
     gene            5401..6120
                     /locus_tag="VF225_08615"
     CDS             5401..6120
                     /locus_tag="VF225_08615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145357.1"
                     /translation="MKLVRYADEPELRAIRVAVLSHPTFPEYMHHNVPGNKYWGRLYE
                     DFPDFQLALVDGDELVAELHSVPTSWDGTDDDLPSGWDEAFLRAFESGREPDVLLALA
                     ISVRPDRQSQGLARRMLEEMRGAAAAGGLRELIAPVRPTLKPRYPLIPIERYLEWRRP
                     DGSHFDPWIRVHERIGGEILAPAPASMVMDNSVSDWEEWTGMQFPVDGDYVVPEMLAP
                     LHVRDGRGHHVEPNVWLRHRV"
     gene            complement(6117..6518)
                     /locus_tag="VF225_08620"
     CDS             complement(6117..6518)
                     /locus_tag="VF225_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145358.1"
                     /translation="MTASIWFYLFPLYILGGLLLVLGTFALLGRIQGGRYLKPIINFM
                     MKLPLVGRLMKKASQAALERQNPELASAVKKLERSGVARDPMRAQQALSRLSASERQA
                     YLDATADQTENIAQNRQMRRQLERARKGKRR"
     gene            complement(6613..7332)
                     /locus_tag="VF225_08625"
     CDS             complement(6613..7332)
                     /locus_tag="VF225_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7145359.1"
                     /translation="MDGLLLGWFAEHGRDLPWRRTRDPYAILVSEVMLQQTQVERVIP
                     RYLAWLEQWPTVAVLAGASAADVIREWQGLGYNRRSLNLHRAAQRIAAEGWPDELTEL
                     PGVGPYTAAAVANFALGRDVLPVDTNVRRIQERTGHRFSSAAGQALMDLGATVCLARV
                     PRCGVCPLSRACPSRGRRYEPLRKQSRFEGSFRQRRARTLRLVAESERPLNDLDREAV
                     ESLARDGLVEVAPCGTLVTLP"
     gene            7365..8318
                     /locus_tag="VF225_08630"
     CDS             7365..8318
                     /locus_tag="VF225_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145360.1"
                     /translation="MQTGKRQAAGLPVEVVLVWVLFAVVTVEVLVTYSRIPSRELYHV
                     SGSGIEGGLSRALVFVNFPVALVALAVLGLIYTRLPGGGYRGVAILAAVLSAAVFWPG
                     IVHQADLDARPVNAVAAIGVLLALVLTGAVASKGGVSTSSWHRADWVRVALAIVLLVA
                     ALPWLAADLGLYSNGVPLLGRLFQSGQFLPQRPGLPNFAPAVHHGHHHGMDGVLLVLT
                     ALLLSRRLARRAALAAYLSLMFCYGVANFANDFWIEQVVKRGWTSWEIPNVTEPRVSV
                     AWSLIVIASVGLWALWVRLVDWSGDEESSLRTEPDRLPARR"
     gene            8266..9255
                     /locus_tag="VF225_08635"
     CDS             8266..9255
                     /locus_tag="VF225_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX7145361.1"
                     /translation="MKRVRFAPSPTGSLHVGNALSAVANRGLGDWMLLRIDDTDPQRN
                     VPGGEEEIIGDLEWLGLVWEEGPVRQSERQDRYREAGAKLGERFDGITLLREDGSATY
                     HLASVVDDIDFGITHIVRGFDHRPNEELHRRLFQALGATPPEFIHHGLILGEDGKKLA
                     KRAPGATVASLRDAGIPGEAVRRYLEELGVPHHDVHYDLPRIRRLAIEAIEAMSDEEL
                     AERAGAPVDVVPALRGARDLNEARDYAQAILTPPKPAKGNAPETLERFRELVERANGT
                     LDAHALVRELKAVGGNLKALRIALTGQERGPELWAVIAALPRDEALRRIDAAL"
     gene            9242..10510
                     /gene="cysS"
                     /locus_tag="VF225_08640"
     CDS             9242..10510
                     /gene="cysS"
                     /locus_tag="VF225_08640"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7145362.1"
                     /translation="MQLYDTLTRGKQELPPTPGPIRMYTCGPTVYQRIHIGNARPFVI
                     SMWLRRWLAERGYDVNLVENITDVNDKIYDAAPGHSAELAAQASQWYVDDTERLGLGR
                     PDHEPKATETIPEIVALIDELVRGGYAYEAGGDVYFRVSRFPEYGRLSGQRPEQLEDD
                     DPSPLKEDPRDFALWKATKEGEDTSWDSPWGRGRPGWHIECSAMAEKFLGPSFEIHLG
                     GLDLVFPHHENERAQSQAAGREFARLWMHNGMLEFVGEKMSKSLGNVASLHDVIERWG
                     RETILVFFLTGHWSKPIEFSDKTLAAAAARVERFREVFRGPSEPAPASAWERFEAALE
                     DDFNTPEALAVMHGWRDHELLRRALDIFGLASLAEQQDAPPEIVELARQRQEARTARD
                     FDEADRLRAEIEAAGWEARDVADGFQLVPR"
     gene            10507..11214
                     /gene="rlmB"
                     /locus_tag="VF225_08645"
     CDS             10507..11214
                     /gene="rlmB"
                     /locus_tag="VF225_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7145363.1"
                     /translation="MTREQVYGRRPVREALRGPREVLELWATERAVKTEPWLQEVERP
                     RLQVKPERELSEAAGTRDHQGVLAWCEPYKYADGWELAAAALPLLACLDQVTDPRNLG
                     AVIRSAEGAGATGVVVPAHGAATVTPAVARASAGAIEHLPVAVVPNLARYLSEVKRGD
                     LWIYAAAGDATTTMWNADLTGGLALVFGAEGKGLRPLVRKTCDGAISIPLDGNVESLN
                     VSVAAALLLYEAKRQRG"
     gene            11207..11641
                     /locus_tag="VF225_08650"
     CDS             11207..11641
                     /locus_tag="VF225_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7145364.1"
                     /translation="MADPTLYLFDGHNLLHAGNYGDLRALRDELASFVALKGARGYLV
                     FDGAGTDESYGPLEVRYAKNADTMLERLAAENRGTEVVCLVSSDSAVRGTSGQEVQKR
                     SSATFIGEMEAVSHNEERPHHLRDTLDEETRAKLERMRRGGD"
     gene            complement(11638..12030)
                     /locus_tag="VF225_08655"
     CDS             complement(11638..12030)
                     /locus_tag="VF225_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7145365.1"
                     /translation="MSAELVAEHGLTINDYEALLHLSRAEEGRMRRVDLAGLLLLTAS
                     GVTRLLDGLETAGLVERASCASDRRVTYAVLTEAGRTRLQQASLSHVAGVRAFFEARY
                     SGEELEQLAALLGRLPGAADATHEDCTG"
     gene            12179..12727
                     /locus_tag="VF225_08660"
     CDS             12179..12727
                     /locus_tag="VF225_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7145366.1"
                     /translation="MSTTTDTRQAPPVGTWALDPIHSAIGFEVDYLAGSFRGQFREVA
                     GKLDAAGEKPVLTGSAKVGSVDVKDENLAGHLQSPDFFDAERYPELSFESSEIDRAGD
                     QITVRGGITIKGVEKPVELTGTIADPITDYTGNEKLSVKLDTTVDRTEFGVNWNTPLP
                     DGKPALADEVKLSAELFFVKEA"
     gene            12733..13290
                     /locus_tag="VF225_08665"
     CDS             12733..13290
                     /locus_tag="VF225_08665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7145367.1"
                     /translation="MKVLAISGSLRRDSYNTKLLRAAEELVPPEVDFVLYQGVKEVPP
                     YDEEDDVQPAPMAVAALRSAIGEADAVLFATPEYNSSIPGSLKNALDWVSRPVATNPL
                     RNKPVAVVGASTGAFGAVWAQAELRKVLAAIGARVVDGEVAVGHAPTRFDEDGRLEDE
                     SLQEQLQETIEALLAETQARSLVLA"
     gene            13419..14075
                     /gene="sigH"
                     /locus_tag="VF225_08670"
     CDS             13419..14075
                     /gene="sigH"
                     /locus_tag="VF225_08670"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX7145368.1"
                     /translation="MAARPATAAQKAQRELEDLQLVMRARNGDGGALDTLIRRYNGFV
                     RLKASSYFLAGGDSEDLVQEGLIGLYKAVRDFRADKETSFRSFAELCVTRQIITAIKT
                     ATRFKHAPLNTYVSFSHTPAGQESDGECSLGDALPGPGVDDPAVCVISTEELQSLVFT
                     LGSGLSQLEADALRLYLEGESYEQMAEDLECDTKTIDNALQRVKRKILTHQKSRQVLL
                     "
     gene            14254..15255
                     /locus_tag="VF225_08675"
     CDS             14254..15255
                     /locus_tag="VF225_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145369.1"
                     /translation="MEEATLEAFAITNELEDDDLAELRGELEEKGIEIVPLEDDAPPA
                     PRVGAAAEVSGTTDSLTLFMNAAGRYQLLTAADEVALAKRIERGDLTAKELMINSNLR
                     LVVSIAKRYQGRGMQLGDLIQEGVIGLNRAVEKFDWRRGFKFSTYATWWIRQACQRAV
                     SNQSATIRIPTHVHERRVKLARARARLQAATGEDPTREELAQATGLDLVYVEEALGAV
                     EASVSLNQSIGSDGDGEFGDLFADQNAEDPSEEAGEALRRQFVRNALGRLPERERRVL
                     ELRFGFDGEATSLEQIGRELSMSRERVRQVERDALAKLQDELAGVVEISPDALVDAA"
     gene            15650..15722
                     /locus_tag="VF225_08680"
     tRNA            15650..15722
                     /locus_tag="VF225_08680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15683..15685,aa:Thr,seq:tgt)
     gene            15725..16336
                     /locus_tag="VF225_08685"
     CDS             15725..16336
                     /locus_tag="VF225_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145370.1"
                     /translation="MPRRSPEASSLPLREELAALVHHYQHLHNDLEREKPEGSTRREL
                     EDKVLDVRERFDRLLEEWVPDEELREQWREYIHNRRPEPDEPAGIEPLAFQGLTDAGS
                     VVQVRGQADEYQVWVDGSLQERIAARKDLSVDKPVLHFRWDNKEIPETFNASEDALNA
                     LATYRDERDASPPWEYASELLADGLIDVHFDLTPRGKRAVAGL"
     gene            complement(16470..>16991)
                     /locus_tag="VF225_08690"
     CDS             complement(16470..>16991)
                     /locus_tag="VF225_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7145371.1"
                     /translation="SVGAAYGVLVALFQWGWGESFLGFQSNGGIASWLPIFMFVILFG
                     LSMDYHVFILSRVREAYDRGLKTEDAVAHGITTTAGVVSSAALVMVGVFAVFALMPIL
                     DMKEMGIGLAAAVLIDATIVRAVLLPATMKLLGDANWYLPRWLEWLPRLEHEASPPAE
                     RAPDAAPAGALGA"
BASE COUNT         2794 a         5638 c         5821 g         2738 t
ORIGIN      
        1 gatcttgcga tgccgcgccg cgtcgcgacg ggtgtcgatc ccaagcgcct cgcgatgatc
       61 gttgcggtgc tcgggcggca cgcccgcatc gcgctcggca gcgcagacgt attcgtgaac
      121 gtcgcgggcg gtgtgcgcat cgacgagccg ggggcggacc tcgcggttgc gctggcgatt
      181 gcgtccgccg caaagggcgt cccgacaaag gaagcgctgg cggcattcgg ggagatcggg
      241 ctcaccggaa ggctgcgtcc tgccgcacag gcggagcgcc ggctcgagga gtgcgcgaag
      301 ctcgggctga agacggttgt cgcgcctgcg gacacgcccg cgacgcgacg caagctcgag
      361 gtgctgcagg cggagaccct gccgaaggcc atcactgctg ggctcagcta gcttcgcgcc
      421 gccaattcca ggtcccgagc ccgagcttgc cgcctgagac tggcaaactg ctgagaatgc
      481 aggaggcaca ggccgcgctg ggcttcacgc gcacgccgtc ggaggacgat ccgcggcgtg
      541 acccggagct gcttgccgcg ctccggcgga tcgcgccggg caccgacctc cgccagggga
      601 tggacgacat catccgctct cgggagggcg cgctgatcgt gatcggcgac ccgaacgagc
      661 tctcgttcct cttctcgggc gggatccgcc tcgacgtccc gttcacgggc cagctcctct
      721 acgagctggc gaagatggac ggcgcgatca ttgtcaatcc gtcgcggtcg aagctcgcct
      781 acgcgaacgt gcagctgatg ccggatccca cgattccctc ggtggagacc gggacgcgtc
      841 accgcacggc cgagcgcgtc gccaagcaga cgggtgccct cgtgatctca atctcgcaac
      901 agcgctccac gatctcggtc tacgtggggc cgacgcgcta ccagctggac gcagtacccg
      961 aggtgctcgg gaagacgaac caggcgctgg ccacgctcga gacgtatggg cagcggcttc
     1021 agcaggtgct cacacggctg acggcgctcg agttccagaa cgccgttgtc ctcgacgacg
     1081 tgctcgtggt gcttcagcgc gcggagatga cgacgcgcat ggccgcggag atcgagcgtg
     1141 actgcgtcga gctcggcgcc gagggacgcc tgatccggat gcagctgaac gagctcgtgg
     1201 gcgaggttcc ggcggagcgc gcgctggtcg tccacgacta ccacgccgat ggatccggcc
     1261 cccgagcgga gctggcgctc gaggggctgc acgcactccc ttaccgggag ctgctcgaat
     1321 cggagcgtct cgccgagctg ctcgggttcc cgcgcgaggt caatccgctc gaccacagcg
     1381 tcgtgccgcg aggtcttcgg atcctgtcgc ggatcccgag actcccggac gccgtcgtca
     1441 agaaggtcgt gggcgacctc ggcgacctcg actcgatcgt ccgtgcgtcc cagcgcgatc
     1501 tggagaggat cggcggcgtc ggcgcggtgc gcgcgcgcga gatccgcgaa ggcctgcgcc
     1561 ggctccagga gcacaatctc gtcgagcggt atctgcacct ctgagtaggg cgacaggcct
     1621 cgccgcgggc tacgcggtct tcgggctctt ctggggctcg tgggctgcgt gtctaccggc
     1681 gattcagcgg gagacgggtg caagcgacac gcagctcggg cttgcgctgc tgtgcgtcgc
     1741 gctcgcggcg ctgccggcca tgctcgtggc ggggcggctc gccgacgtgt tcggcgcccg
     1801 gctcgtcccg atcttcctcg tcttcttcgg cgccgtggaa tggcttcccg ggctcgcgca
     1861 ctccgtgccg atgctcgtcc tcttgctggc cgtcatcggg gtcggcaccg gcctgctgga
     1921 catcgcgatc aacgcccatg ccgcgcggct cgaggcgctg cacggcatcc ggatcatgga
     1981 cgggctccat gccgccttct ccggcggcgt cctcgtcggt ggcatcggct cggggctgct
     2041 gcggcacgcc ggcgcgcacc cggtctggat cctggccggc gtcggcctgg tcaccgcgct
     2101 gaccgcgctc gcgaacctgg ggccgtaccc accgcttgca ccgcggcagc gacgcgcgcg
     2161 cctggcgcgg cctctgctca tcgtcgggct cgtgctcgcg ctcgcattcc tcgtcgagaa
     2221 cgggctcgaa gcgtggagcg cgctcttcct cgagcggacg ttgaattcga gcgccgcggt
     2281 cagcggcctc gggccgggct tgttcgccgg cgcaatgctg actggccgcg tcctggcaca
     2341 acgggtggag tggccgtccg tcgccgggcg gatgctcttt gccggacttg cggcggcagc
     2401 cggtctcgcc ctcgccgcca cggcgcagca cgccgcggtg gctctggccg gcttcgtcgt
     2461 ggcgggcgga ggcctggccc tgtcggcgcc gactctcttc ggcctggccg gacgggttgg
     2521 gggcgagggc ggccggggcg cggctctttc gaccgtcgcg atcctcggct atctgggctt
     2581 cgtagccggc cccgcgctca tcggggcggt gtccggggcg tcgagcctgc ggggcgggct
     2641 cctcttcctt tgcgccgtgg cggtgatcct ggcggcctgc gcgccgatcc tccgccgggt
     2701 agtccgcgaa atcggcggtt agcagggatt tcccgaaaat gtggccattt ccagcgcctt
     2761 ttgctagact tgtcctccta ggccggcgga accacccgcc ggcttcgcgg tcacaaaagg
     2821 acggaaaggg gcgggcttgt acaagatcgg cgacaaggtg gtgtatccgc accacggagc
     2881 aggcaccgtt gtcaagaggg aaaagcgtga ggtgctcggc gaaaagcgtg agtacctgac
     2941 gatcaagatc ctccacaacg acatgacagt gaacgtcccg tccgagaacg cggaggccgt
     3001 tggcctgcgc aaggtgatcg gcgaggacat ggtgaaggtc gtcgtcaagg cgctgaccgg
     3061 tggcggcacg cagatgccga agaactggaa ccgccgtttc aagcacaacc gcgacaagat
     3121 gaagaccggc gacatcctcg agctcgcgga ggtcgtccgg aatttgtctc tacgtgacag
     3181 cgaaaagggc ctgtcgaccg gcgagaagca gatgttcgtg aaggccaaga agatcctggc
     3241 cagcgagttg atgtacgcga aggacatgga cgaggaggag gctgccgagt ggctcgacgg
     3301 cgttctcgtc gggagcgcca acggcagcac caacggtcgt aagaagacca ccgccgcgaa
     3361 gcccgcgaag gttgcgtcca agacttcagc gaagccggca gcccgcaaga ccgcggcgac
     3421 ggcggcgaag gccaagccga agacaaaggc gcgcgcctcc tcgaggtgag cgtctgggca
     3481 gtcctcgctg cagcgggtag cggggaccgg ctcggtggcg atcgtccgaa agcgttcgcg
     3541 cgtctcggtg acgagccgct gctcgcccag agcctgaagc gtctggacga ctccgactgg
     3601 gtggacttca tcgtcgtggc cgctcctccc ggctgggagg agcctgcgat cctgctcgcc
     3661 gaggagctcg gctgcagcaa ggtgagcgca tgcgtgaccg gcggcgcgac acggggccag
     3721 tcggttgcga gggcgctggc agaagtgcca gaagacgctg cagtcgtgct cgtccatgac
     3781 gcggcgcgcc ccctggtgtc ggacgaggtg atcgaacggg tgctgacggc gttgaacgag
     3841 ggctgggacg gagcgttgcc cggactgcag ttggcggaca cgatcaagca tgtcgatgag
     3901 gagggccgga tcgtcgagac actgaatcgc gacgagctgc gggcggtgca gaccccgcag
     3961 gcattcgtcg cttcggtgct gcgtgatgcc tatgccaagg gagatacggc ggtcgcgacc
     4021 gactgttcgt ccctggtcga ggcccgcggc ggacgcgtca aggtcgtcga aggcgacccg
     4081 aagctgatca aggtcacgga tgccgccgat ctcggtcgca tcgctgcgct gctgtgatcg
     4141 tcgactacca catgcatctc cggaacggga gtggggagat cgcacacgag acctcggcga
     4201 tcgatccctt cgtcgaatcg gctcgtgagg cgggcgtcga cgagatcggg ttcacggagc
     4261 acatctacta cttcaagcaa acacgctcgt tgtggacggt gccgtaccac acggagcgct
     4321 gcgtgtacga cctcgaggcc tacgtttcgg ctgtcgtgac ggcgcgcggc cgtggactac
     4381 ccgtgaagct cgggctcgaa gtcgactacg tgccgggccg cgaggacgag acgcgtgagc
     4441 tcctggcgcc ctatccgtgg gactacctgc tcgggtcgct gcactacatc ggctcgctcg
     4501 gcgtcgatga cgagccacgg ctggtcgacg cggtgggggt ggaggacgct tggcggttgt
     4561 atttcgagac gctcgcagcg gctgcgcgga gcgggctctt cgactcgtta tcgcatcccg
     4621 atctcatcaa gatcttcggc gctcgcgcgg cgtcgttcga ctacgagccg gtggttgctg
     4681 cgatcgccga ctccggcgtc gcagtggaag tgtcgacagc cgggctccac aagcccgttc
     4741 atgagctcta tccgcaccct gagtttctcg ccgcatgtcg cgcactcgac attccggtca
     4801 cgctcgcctc cgatgcgcac acgccggacg ttgttggacg agacttcgat cgggcgctcg
     4861 agctcctgcg ttcggccggc tacgacacgg tgacggtgtt cgagcagcgc cgcgcacgac
     4921 aggagccatt gggatgagcc tgcgggtggg aatcggcgtc gacgcgcacc agttcgagga
     4981 cggcatgcca ctcgttttgg ggggtgtcgg gttcgagtcc tcgcgcgggt tggcgggcca
     5041 ctcggacgga gacgtgatca cgcatgcgct gatcgacgcc gtcctcgggg cggcgggcct
     5101 cggtgacatc gggtcgctgt ttccgtccga ctcgtcgacg cccgccggga tctcgtcgct
     5161 ggatcttctg cgtgacgcag tcgcgcgtgt gcgggacgca ggctacgcgg tcgtgaatgc
     5221 cgattgcgtg ctgatcggcg aggagccgcg catctctggc cgccgcacgg agatggccga
     5281 cgccttgagc gccgtcgtcg gcgcgccggt gaacgtgcgt gcgacgacca ccgacgggct
     5341 cggcttcacc ggacgaggcg aaggtctcgg cgcccaggcg gtggccctcg tcgaatcccc
     5401 gtgaagctcg ttcgctacgc agatgagccg gagttgcggg cgatccgagt cgcagtcctc
     5461 tcgcatccga cgttcccgga atacatgcat cacaacgttc ccgggaacaa gtactggggc
     5521 cggctgtacg aggacttccc ggacttccag ctcgcgctcg ttgacggcga cgagctggtt
     5581 gccgagttgc attccgtgcc gacctcgtgg gacgggacgg atgacgatct tccgtccggg
     5641 tgggacgaag cgttcttgcg tgcgttcgaa agcgggcgcg agccggacgt gctcttggcc
     5701 ctcgcgatct ccgtgcgccc ggaccgccaa tcgcagggac tcgcgaggag gatgttggag
     5761 gagatgcgcg gcgcggctgc cgccggtggg ttgcgcgagc tgattgctcc ggtgcggccg
     5821 acgctgaagc ctcgctatcc gttgattccg atcgagcggt acctggaatg gcgccggccg
     5881 gacggctcgc atttcgatcc gtggatccgc gttcacgaac gtatcggcgg cgagatcctc
     5941 gcgcccgcgc cggcatcgat ggtcatggac aattccgtgt cggactggga agagtggacc
     6001 gggatgcagt tcccggttga cggcgactac gtcgtccccg agatgcttgc gccgctgcat
     6061 gtgcgcgacg gccgcggcca ccatgtcgag ccgaacgtgt ggctacggca ccgggtctag
     6121 cggcgcttgc ccttgcgggc ccgctcgagc tggcggcgca tctggcggtt ctgggcgatg
     6181 ttctcggtct ggtcggcggt tgcgtcgagg taggcctggc gctcggaagc gctgagccgt
     6241 gagagcgcct gttgggcgcg catcggatcc cgggcgacgc ccgagcgttc aagcttcttg
     6301 accgcgcttg ccagctcagg gttctggcgc tcgagcgctg cctgcgacgc cttcttcatc
     6361 agccgtccga cgagcggcag cttcatcatg aagttgatga tcggcttcag gtagcggccg
     6421 ccctggatgc ggccgaggag ggcgaacgtg cccagcacga gcagcaggcc gccgaggatg
     6481 tagagcggga agaggtagaa ccagatggag gcggtcacgg ctcggatcgt agcccagccg
     6541 ccttgacgcg gcgccaccga gcccgcccag cgagacgtgc cctcaaaggc gacgtcccgg
     6601 tgtcagacac cactacggaa gtgtgacgag cgttccacag ggggcgactt cgaccaggcc
     6661 gtctcgggcc agcgattcga cagcctcgcg gtcgaggtcg ttcagcggac gctccgactc
     6721 tgcaaccagt cgcagcgtcc gcgcacgccg ctgacggaac gagccttcga agcgcgactg
     6781 cttgcgcagc ggttcgtagc gacggccgcg ggacggacac gcccgcgaca acggacagac
     6841 cccgcagcgc gggacgcgcg caagacacac cgttgcgccg agatccatca gcgcctgacc
     6901 cgccgccgac gagaagcggt ggcccgtccg ttcctggatg cgtctgacgt tcgtgtcgac
     6961 cggtagcacg tcacggccga gcgcgaagtt cgcgaccgcc gcagccgtgt acgggccgac
     7021 gcccggcagc tccgtcagct cgtcgggcca accctcggct gcgatcctct gcgcagcgcg
     7081 atggagattc aggctgcggc ggttgtagcc gagcccttgc cactcccgaa tgacgtcggc
     7141 cgccgacgcg ccggccagca ctgccacggt cggccactgc tccaaccagg cgaggtaacg
     7201 cgggatcacg cgctcgacct gcgtctgctg cagcatcacc tccgagacga ggatcgcgta
     7261 cgggtctcgc gtccgcctcc acggtaggtc gcgtccgtgc tcggcgaacc agcccaggag
     7321 tagaccgtcc acggtcgcga gcgtaccctg gaaggcggcc gatcttgcag acaggaaagc
     7381 gacaggccgc tgggctgcca gtcgaagttg tcctcgtctg ggtgctgttc gccgtcgtga
     7441 ccgtggaggt cttggtcacg tacagccgca tcccgtctcg cgagctctac cacgtgtccg
     7501 gcagcggtat cgagggcggg ttgagccgcg cgctggtgtt cgtcaacttc ccagtagccc
     7561 tagtcgcact cgccgtgctc gggctcatct acacacggct cccgggaggc ggttaccgag
     7621 gcgtcgcgat cctcgccgcc gttctctccg ccgcggtgtt ctggcccggg atcgtccacc
     7681 aggctgatct cgatgcaagg cccgtcaatg ccgtcgcagc aatcggagtt ctgctggcgc
     7741 tcgtgctcac cggagcagtt gcgagcaagg gtggcgtatc gacaagctcc tggcacaggg
     7801 cggactgggt gcgcgttgca ctcgcaatcg tcttgctcgt cgcggccctg ccctggctcg
     7861 cagctgatct cggtctctac tcgaacggcg ttcccctgct cggccggctg ttccagagcg
     7921 gccaattcct gccgcagcgg ccgggcctgc cgaacttcgc cccggcggtc catcacggcc
     7981 accatcacgg gatggacggt gtcctgctcg tgttgacggc gctcctgctc tcgcgccggc
     8041 tggcaaggcg ggcggcgctc gcggcgtacc tctccctgat gttctgttac ggcgtcgcca
     8101 acttcgccaa cgacttctgg atcgagcagg tcgtcaaacg cggctggacg agctgggaga
     8161 tcccgaacgt gaccgaaccg cgcgtgagcg ttgcctggag cctgatcgtg atcgcgtcag
     8221 tgggcctgtg ggctctgtgg gtccggctcg tagactggag cggagatgaa gagagttcgc
     8281 ttcgcaccga gcccgaccgg ctccctgcac gtcggtaacg ccctcagcgc ggtcgccaac
     8341 cgtggcctcg gggactggat gcttttgcgg atcgacgaca ccgacccaca gcggaacgtg
     8401 cccggcggcg aggaggagat catcggcgac ctcgagtggc tcggcctcgt ctgggaggaa
     8461 gggcccgtcc gccagagcga gcggcaggac cgttaccgcg aagcgggcgc caagctcggc
     8521 gagcgcttcg acgggatcac gctcctccgc gaggacggca gcgccacgta tcacctcgcg
     8581 agcgtcgtcg acgacatcga cttcgggatc acgcacatcg tccgcggctt cgaccaccgc
     8641 ccgaacgagg agctgcaccg ccgcctgttc caggcgctcg gcgcgactcc gcccgagttc
     8701 atccaccacg ggctgatcct cggcgaggac ggcaagaagc tcgccaagcg cgcacccggt
     8761 gcgaccgtcg cgtccctgcg cgacgccggc attcccggag aggccgtccg ccgctatctc
     8821 gaggagctcg gcgttccgca ccacgacgtc cattacgacc tcccgcgcat ccgccggctc
     8881 gcgatcgagg cgatcgaggc gatgagcgac gaagaactcg ccgagcgagc gggcgctccg
     8941 gtcgacgtcg tccctgcact gcgcggcgcg cgcgacctca acgaggcgcg cgactacgcg
     9001 caagcgatcc tcacaccgcc gaagccggcc aagggcaacg cgcccgagac cctagagcgt
     9061 ttccgcgagc tggtcgagcg cgcgaacggc acgctcgacg cgcacgcgct cgtgcgcgag
     9121 ctgaaggcgg tcggcggaaa cctgaaggcg ctccgaatcg cgctgaccgg acaggaacgc
     9181 ggcccggagc tctgggccgt gatcgctgcg ctgccgcgcg acgaagcact gcgcaggatc
     9241 gatgcagctc tatgacacgc tgacacgggg gaagcaggag cttccaccca cgccgggccc
     9301 gatccgcatg tacacctgcg gaccgaccgt gtaccagcgc attcacatcg gcaacgcgcg
     9361 gccgttcgtg atctcgatgt ggctgaggcg ctggctcgcg gaacggggct acgacgtcaa
     9421 cctcgtcgag aacatcaccg acgtcaacga caagatctac gacgctgccc cagggcacag
     9481 cgccgagctc gcggcgcagg cctcgcagtg gtacgtcgat gacaccgaga ggctcggtct
     9541 cggccggccc gaccatgagc caaaggcaac ggagacgatt ccggagatcg ttgcgctgat
     9601 cgacgagctc gttcgcggtg ggtacgccta tgaagccggc ggtgacgtct acttccgtgt
     9661 ctctcgcttc cccgagtacg gccgcctgtc ggggcagcgg ccggagcagc tcgaggacga
     9721 cgatccaagc ccgctcaagg aggacccgcg cgacttcgcc ctctggaagg cgacgaaaga
     9781 aggcgaggac acctcctggg actcgccctg gggtcgcggt cgcccgggct ggcacatcga
     9841 gtgctccgcg atggcggaga agttcctcgg cccgagcttc gagatccacc tcggcggcct
     9901 cgacctggtc tttccgcacc acgagaacga gcgcgcgcaa tcgcaggccg cgggtcgcga
     9961 gtttgcacgt ctctggatgc acaacggaat gctcgagttc gtcggcgaga agatgtcgaa
    10021 gtcgctcggt aacgtcgcgt cactgcatga tgtgatcgag cggtggggcc gggagacgat
    10081 cctcgtcttc ttcctcaccg gccactggtc gaagccgatc gagttctcgg acaagacgct
    10141 cgcagccgcc gccgcgcggg tcgagaggtt ccgcgaggtg ttccgcggcc cctccgagcc
    10201 tgcgcctgcg agcgcgtggg agcgcttcga ggctgcgctg gaggacgact tcaacacgcc
    10261 ggaggcgctc gcggtcatgc acggctggcg cgaccacgag ctcctgcgcc gagccctcga
    10321 catcttcggc ctggcctcgc tcgcggaaca gcaggacgcc ccgcccgaga tcgtcgagct
    10381 ggcgcgccaa cgacaggagg cacgcaccgc acgcgacttc gacgaggccg accggctccg
    10441 cgccgagatc gaagccgccg gctgggaggc gcgcgacgtg gctgacggct tccaactcgt
    10501 tccgcggtga cacgcgagca ggtctacggc cgccgcccgg tacgggaagc gctgcgcggc
    10561 ccccgcgaag tgctcgagct ctgggcgacg gaacgcgccg tcaagacgga gccctggctg
    10621 caggaagtcg aacgccctcg cctgcaggtg aagccggagc gcgagctcag cgaagcagca
    10681 ggcacccgcg accatcaggg cgtgctcgcg tggtgcgagc cgtacaagta cgcggacggg
    10741 tgggagctcg cagccgccgc gcttccgctg cttgcgtgtc tcgaccaggt gaccgacccg
    10801 cgcaacctcg gtgccgtcat ccgcagtgcc gaaggggcgg gagcgaccgg agtcgtcgtc
    10861 cctgcgcacg gcgccgcgac ggtgacgcct gcggtcgcgc gggcatccgc cggcgcgatc
    10921 gagcatctgc ccgtcgccgt cgttccgaat ctcgcccgct atctcagcga ggtgaaacgc
    10981 ggcgacctct ggatctatgc agcagccggc gacgcgacga cgacgatgtg gaacgccgac
    11041 ctcaccggcg ggctcgcgct cgtcttcggc gcggaaggca agggcctgcg tccactcgtg
    11101 cgcaagacct gcgacggcgc gatctcgatc ccgctcgacg gcaacgtcga atctctgaac
    11161 gtcagcgtcg cagcggcttt gctgctctac gaggcgaaac gccaacgtgg ctgatcccac
    11221 tctctatctg ttcgacggcc acaacctcct gcacgccggg aactacggcg acctgcgagc
    11281 gctccgcgac gagctcgcca gcttcgtcgc gctcaagggc gcgcgtggct acctcgtttt
    11341 cgacggcgcc ggtactgacg agtcgtacgg cccactcgag gtgcgctacg cgaagaacgc
    11401 tgacacgatg ctcgagcgac tcgctgccga gaaccgaggc accgaggtcg tctgcctcgt
    11461 ctcgtcggac agcgctgtgc gtggcacctc cgggcaggag gtccagaagc gctcgtccgc
    11521 gaccttcatc ggcgagatgg aagcggtctc gcacaacgag gagcggccgc atcatctccg
    11581 cgacacgctc gacgaggaga cccgggcgaa gctggaacga atgcgccgcg gaggcgacta
    11641 gcccgtgcag tcttcgtggg tcgcgtcggc ggcgccgggg agccggccga gcaatgctgc
    11701 aagctgctcg agctcttctc cgctgtagcg cgcctcgaag aacgcccgta cgcctgcgac
    11761 atgtgacagg gaggcctgtt gcagtctcgt gcgccctgcc tcggtgagga cggcatacgt
    11821 gacccggcgg tcgctggcgc aggacgcccg ctcgaccagt ccggcagtct cgaggccgtc
    11881 gaggagccgg gtcacgcccg aagccgtgag gagcagcagg cccgccaggt cgacccgcct
    11941 catccgaccc tcctcggccc gcgacagatg gaggagcgcc tcgtagtcgt tgatcgtcag
    12001 cccgtgctcg gcgacgagct cggcgctcat ggcccgggtc accgaggcgt acccccggat
    12061 gagccggatc caggaatcca gggcagaatc ttgttgctga agcaatactt gactagacaa
    12121 tctttcctcg ctatattggt tgcgtactca actatagcct actcaacaag gagagtaaat
    12181 gagcaccacg acagacaccc gccaggcacc gccggtcggc acctgggcgc tcgaccccat
    12241 ccactcggcg atcggcttcg aggtcgacta cctggccggc tcgttccgcg gccagttccg
    12301 cgaggtggcc gggaagctgg acgccgccgg cgaaaagcct gtgctcacgg gctcggcgaa
    12361 ggtcggcagc gtcgacgtta aggacgagaa cctcgccggc caccttcagt cccccgattt
    12421 cttcgacgcc gagcgctacc cggagctcag cttcgagtcg agcgagatcg accgcgcggg
    12481 cgaccagatc acggtccgcg gcggcatcac gatcaagggc gtcgagaagc cggtcgagct
    12541 gacgggcacg atcgccgacc cgatcaccga ctacaccgga aacgagaagc tctcggtcaa
    12601 gctcgacacg acggtcgacc gcaccgagtt cggcgtcaac tggaacactc cgctccccga
    12661 cgggaagccg gcactggccg acgaggtcaa gctctcggcc gagctcttct tcgtcaagga
    12721 ggcgtaagag ggatgaaggt tctcgcgatc tcgggcagcc tccgccggga ctcgtacaac
    12781 acgaagctcc tccgcgcagc ggaggagctt gtccctccgg aggtcgactt cgtcctctac
    12841 cagggcgtga aggaagtgcc gccgtacgac gaggaggacg acgtccagcc ggcgcccatg
    12901 gcggtagcgg ccctgcgcag tgcaatcggc gaggcggacg cggttctgtt tgcgacgcct
    12961 gaatacaact cgtccattcc gggctcgctg aagaacgcac tcgactgggt ctcccggccg
    13021 gtcgcgacga acccgctccg gaacaagccc gtggcggtcg tcggtgcaag caccggagcg
    13081 tttggagccg tctgggccca ggccgagctg cggaaagtcc tcgccgccat cggcgcgcgg
    13141 gtggtggacg gcgaggtcgc cgtcgggcat gcgcccacgc ggttcgacga ggacggccgg
    13201 ctcgaggacg agtcgctgca ggagcagttg caggagacga tcgaggcgct tctggccgag
    13261 acccaggcca ggtcgctcgt tctggcctga cgaacatacg ttcgtaacat cctgggtcca
    13321 ctgcaaacgc cttgtaaaga ccagcggtcc ttgctatctt ctccctcaac cttagggtga
    13381 gacttcgtgt cttgcccctg accccggggg aggccaggat ggccgcacgg cccgcaaccg
    13441 ctgcccagaa ggcccagagg gagctcgaag acctccagct ggtgatgcga gcccggaacg
    13501 gcgacggtgg ggcgctcgac accctgatcc ggcgctacaa cggcttcgtc cggctcaagg
    13561 cgagctcgta ctttctcgcc ggcggggact ccgaggatct cgtgcaggaa ggcctgatcg
    13621 ggctctacaa ggctgtacgc gacttccgtg ccgacaagga gacgtcgttc cgcagcttcg
    13681 cggagctgtg cgtcacccgc cagatcatca cggccatcaa gaccgctacg cgcttcaagc
    13741 acgctcccct gaacacctac gtttccttca gccatacgcc agccggccag gagtcggatg
    13801 gcgagtgctc cctgggggat gccctccctg ggcccggcgt agacgatccc gcggtctgcg
    13861 tgatctcgac cgaggagctc cagagcctcg ttttcaccct gggtagcggc ctctcgcagc
    13921 tcgaggcgga cgccctccgg ctctatctgg agggtgaatc ctacgagcag atggcggaag
    13981 atctagaatg cgacacgaag acgatcgata acgcgctcca gcgggtaaag agaaagattc
    14041 ttacccacca gaagtcccgg caggtcctgc tttaaaccgg gaattttggc cgcctctacc
    14101 gcgttttaga aggtacggag ctttaggcac ggtccggatt ggaccgccgc gaacgggctc
    14161 ccgaccgaaa tcgagaggag atgaaccaag ctgaccaacc ttgctgaact tgatttgcag
    14221 tctttcctcc acgaggccga ggagctcggc tccgtggaag aggcgaccct cgaggcgttc
    14281 gctatcacga acgagctcga ggacgacgac ctggcggagc tccgtggtga gctcgaggag
    14341 aagggcatcg agatcgtccc gctcgaggac gacgcacccc cggcaccccg cgtgggggct
    14401 gctgcagagg tttccgggac gacggacagt ctgaccctgt tcatgaacgc cgcaggccgc
    14461 tatcagctgc tcaccgctgc cgacgaggtc gcgctcgcga agcgcatcga gcgcggtgac
    14521 ctcactgcca aggagctcat gatcaactcg aacctccggc tcgtggtctc gatcgcgaag
    14581 cggtatcagg gccgcggcat gcagctcggc gatctgatcc aggaaggcgt gatcgggctg
    14641 aaccgcgcag tcgagaagtt cgactggcgc cgcggcttca agttctcgac ctatgccacc
    14701 tggtggatcc gccaggcctg ccagcgggcg gtgtcgaatc agtcggcgac gatccggatt
    14761 ccgacgcacg tccacgagcg gcgcgtaaag ctcgcgcgtg cgcgggcgcg cctgcaggct
    14821 gcgaccggag aggatccgac gcgtgaggag cttgcccagg caaccgggct cgacctcgtg
    14881 tacgtcgagg aggctctcgg cgcagtcgag gcatccgtgt cgctgaacca gtcgatcggc
    14941 tccgacggcg acggcgagtt cggcgatctg ttcgccgacc agaacgccga ggatccgtcc
    15001 gaggaggctg gagaggccct ccgccgccag ttcgtccgga acgccctcgg gcgtctgccg
    15061 gagcgcgagc gtcgtgtgct cgagctgcgc ttcggattcg acggcgaggc cacttcgctg
    15121 gagcagatcg gccgcgagct gagcatgagc cgcgagcggg tccgccaggt ggagcgcgac
    15181 gcgctggcca agctgcagga cgagcttgcc ggcgtcgtcg agatctcacc ggacgcgctc
    15241 gtcgacgcgg cttaattcga ctcagagatc gggcgcgtgc atgtcacgcg tccgatctcg
    15301 taatcccgcg catatttgat ccggagcctc tgacaccgtt tcgggcttcg gtcactcgga
    15361 gcgacccatt ccacctaacg gctacgtcgt cgatcggtat caggtagatg ccgctcgttt
    15421 cagggcaata gacggcgaaa tactcaattt ggcctacgta gtcccgcgcc gctgatgcgg
    15481 ggtttgagtg atgggcgtcc gcgtgtccga tgccctgcaa ggcgatcgcg atggctaacg
    15541 cggaacggtc gccaatgtcc ttcggatctc catgcgatca gcttgccgag ccaatcggct
    15601 gggccgtgtc caaaaccgac accaagaact tcgcttacca tgagggcgtg ccggagtagc
    15661 tcagttggta gagcaacggt tttgtaaacc gtaggccgtg ggttcgagtc cctcctccgg
    15721 ctccttgccc agacgctctc ccgaggcttc gtctcttcct ctgcgggagg aactcgcagc
    15781 gctagtccac cactaccagc acctgcacaa cgacctcgag cgggagaagc ccgaggggtc
    15841 gacgcggcgc gagctcgagg acaaggtgct cgacgttcgc gaacggttcg accgcctgct
    15901 cgaggaatgg gttccggacg aggagctccg cgagcaatgg cgcgagtaca tccacaaccg
    15961 ccggcccgag ccggacgagc ccgccgggat cgagccgctt gccttccagg gcctcaccga
    16021 cgcgggctcg gtggtgcagg ttcgcggcca ggcggacgag taccaagtct gggtcgacgg
    16081 ctcgctgcag gaacgcatcg cggcgcggaa ggatctctcc gtcgacaagc cggtcctgca
    16141 tttccgctgg gacaacaagg agatcccgga gacgttcaac gcctcggagg atgcgctgaa
    16201 cgcgctcgcc acgtaccgcg acgagcgcga cgcatcgcca ccctgggagt acgcgtcgga
    16261 actgctcgcc gacggcctga tcgacgttca cttcgatctc actccgcgcg gcaagcgcgc
    16321 agtcgccggg ctctaatcca caacatccac agctgtctgt ttggtgacat gtcttggggt
    16381 cagaccccag gacgagtcgt ttgcggacag gaacgaaaag cgccccgccg aaaggcgggg
    16441 cgcttttctt gcgaaggagc ggggtggagc tacgcgccga gtgcgcctgc cggagcggcg
    16501 tcgggtgccc gctccgccgg aggagacgcc tcgtgctcca gcctcggcag ccactcgagc
    16561 caccgcggca ggtaccagtt ggcgtcgccc agcagcttca ttgttgccgg caggaggact
    16621 gcccggacga tcgtcgcgtc gatcaggacc gccgcggcga ggccgatgcc catctccttc
    16681 atgtccagga tcggcatcag cgcgaacact gcgaacacgc cgaccattac gagcgcggca
    16741 ctcgagacga cgccggccgt ggtggtgatg ccgtgtgcca ctgcgtcttc ggtcttcagt
    16801 ccgcggtcgt acgcctcccg cacccggctc aggatgaaca cgtggtagtc catcgagaga
    16861 ccgaacagga tcacgaacat gaagatcggg agccatgagg cgatgccgcc gttcgactgg
    16921 aagccgagga agctctcgcc ccagccccac tggaagaggg cgacgagcac gccgtacgcc
    16981 gcacccactg a
//
LOCUS       DASVEU010000206        11019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1308302,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000206 DASVEU010000000
VERSION     DASVEU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308302"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF225_08695"
     CDS             complement(<1..264)
                     /locus_tag="VF225_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145372.1"
                     /translation="MPYFICPNCKQRSIDHDRLQEIDNVPVACDRCGFGFLFELMDDY
                     YPAPNTGFVVCDKDGRILASGRGVFELSGFREQELLGADLVKRF"
     gene            complement(283..1191)
                     /locus_tag="VF225_08700"
     CDS             complement(283..1191)
                     /locus_tag="VF225_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7145373.1"
                     /translation="MAEWIDLIDPTPEELRERLPRELYETALDRLLAPAEHTDEPRPT
                     LQGHGGYIFGIFLVAVGVPEEDRVFYQEVDVVVTHDVLVTVSKTPPGEHPYDPRPARE
                     SCRPDDSAGMMFYRLVDDIAERYLDLVDQLDEEIDELEDGVETAPAPQTRDRLSELRH
                     DLLHIRRTLSPTRDAIRRVVDNVVEVEEGEEVFPHDVEVAFNSAYDKLLRAFDGLELS
                     RDLIASVRDYLQAKIANDQNEVTKKLTALASMLLVPTFIVGVYGQNFKHHFPELGWQW
                     GYAWSWGLIVVTTIAQFVFFRRRSWI"
     gene            complement(1227..2195)
                     /gene="trxB"
                     /locus_tag="VF225_08705"
     CDS             complement(1227..2195)
                     /gene="trxB"
                     /locus_tag="VF225_08705"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7145374.1"
                     /translation="MTDSDVREMIIIGGGPAGYTAALYAARANLRPLVIEGFNWGGQL
                     MITSDVENYPGYADGVLGPEMMQEFRRQAERFGTDFITDDVTKVDFTERPHRVWVGDE
                     EYRAETVIVATGATARQLGLESERRLQGRGVSYCATCDAAFFPQKHVVVVGGGDSAME
                     EATFLARFAAKVTIVHRRDEFRASQIMEDRARGNDKIDWVTNAVVDAVLGDERVTGVR
                     LRDVNTDETWELDADGVFAAIGHDPNTALFVDQLEHDEAGYLITKAHSTETNVPGVFA
                     VGDVQDHVYRQAVTAAGSGCMGALDAEKYLAALQGHLMAAEAAPRS"
     gene            2324..3457
                     /locus_tag="VF225_08710"
     CDS             2324..3457
                     /locus_tag="VF225_08710"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX7145375.1"
                     /translation="MAGTVTWSLLRELAGFRAEKGCAISLCLNLDPSDVPTAGDAQTR
                     MNSMLNAADKTDRTDLTHEQRSALKADFERIATWFDDDFERDGSQGLAVFAAGLDNFW
                     RTLPLPQPVRDSAKVGRDFYLAPLVPLVSRGDGTIVAVVGREQGQLYRLRAGRLEEIA
                     EHYDEQPGQHDQGGWSQGRYQRHIEKLVQEHLKGLAQELDRSKRRLQSPKVVLVCSEE
                     MRSEFKDALSAEVRAALVGWAPAQAQAGPAELLQAVTPVLEEAESKDEAEVIERWREE
                     AGRNARAASGWEQTLEAASDARVELLLFQEGADRPAFRCPACGRAAVSEGSCPLDGTQ
                     LEPTDAGLDLAVHQTLAHGGAVWAIRHHEDLAPVEGIGALLRF"
     gene            complement(3467..4504)
                     /locus_tag="VF225_08715"
     CDS             complement(3467..4504)
                     /locus_tag="VF225_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7145376.1"
                     /translation="MTDVILILLLGVGLAAALAWLARALRDLRTDWSSQLSERNAEVD
                     RRLVGVTETMDRRLGELDTKVDRRLESASQTTNKIHERLGKVDEATTQMLERAKDLAR
                     LEQALRPPKARGGFGELLLENLLRDRLPPSAYEMQYTFDSGERVDAIVRADRTIPVDS
                     KFPLDNYIRLVDAETDDERQLAERQFGRDVKQHIDAIAGKYIRPDEGTYDFAFMYIPV
                     EGVYYELACGKTGALLSYAHERRVFPVSPTTFTAYLQVIALGLRGMQIEEHAHEVMAY
                     VADLRRDFDRFADDFDKVGTHLGHAQSKYHEAGKRLDKFGTKLERAVEEQEELEGETV
                     LELPGVAADAA"
     gene            complement(4579..4896)
                     /locus_tag="VF225_08720"
     CDS             complement(4579..4896)
                     /locus_tag="VF225_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145377.1"
                     /translation="MVPWRYGGTRPASARERLVDREGFILGTSKRVPNKKREAKMGLL
                     DRLLGRGKKAAGDLKGDASMRREGAAQERQGAAEDRAAKHEEMAQEQRNQAAEAHAER
                     ENT"
     gene            5073..5771
                     /locus_tag="VF225_08725"
     CDS             5073..5771
                     /locus_tag="VF225_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145378.1"
                     /translation="MAKKVRTPPPPRRVQAPQRRETRRPPRGSQRSPWIYAVGGALAA
                     AVIAAAVVGILVASGGGSTTKATAANYNALPGIRRTKAPWPPEYLYLADRLAPLGLTT
                     LGGHNGLVLHFHVHIDIFVNGKKVKIPALVGINVGAGWLTELHTHDARGVIHIEAQKS
                     RDFTVGQFFSEWAVYLDSHSIGGYSGMRWYLNGKLQTGNPATLVFKPHQEIAFVVGKA
                     PAKIPSSYAFRAGE"
     gene            5800..5888
                     /locus_tag="VF225_08730"
     tRNA            5800..5888
                     /locus_tag="VF225_08730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5835..5837,aa:Leu,seq:taa)
     gene            6073..6960
                     /locus_tag="VF225_08735"
     CDS             6073..6960
                     /locus_tag="VF225_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145379.1"
                     /translation="MAIREVRASGLEYLGLATQLLQRARLADAEAGLWEAADLQWWWR
                     TPRSSDAIDQFFWIDDEGPVAGLVLTDWGRSWGCDLIVVPGVSTVPLSTVWARAVEAI
                     DAFGLEAVEVLARDDDVELRNLLSGAGFVPDDNQSGIAWMNAQEQPDVAALPEGFVLV
                     DRAQETNTPHPMRRRSGEEVEARLLRCSLYDPGLDLAVEASDGQAAGYALFWFDPVTN
                     VGLVEPMRVEVAYQRRGLARALLTAGLDRLARRGARRLKVGYATDVARRLYVGAGFRV
                     TSTSTSYGRRGPALHRVET"
     gene            6970..8259
                     /locus_tag="VF225_08740"
     CDS             6970..8259
                     /locus_tag="VF225_08740"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7145380.1"
                     /translation="MTELEELSAEEQPLEWDTPLFRTALMQFEQAIPYAEVNESIAER
                     LRYPERAVMVTVPIRRDEGHVDVFPAYRVQHSSVLGPTKGGIRYDPHVTLGECTALAV
                     WMTWKCALLRLPYGGAKGGVRCNPRELSARELERLTRRYTSELRGVIGPQEDIPAPDM
                     ATNEQTMAWIMDTYSMQVGHAVPEIVTGKPISLGGSLFRHEATGAGVVMVIERACHRL
                     GWDLAEQRCVVQGFGNVGGIAAQELVSKGAAVLAVSDVSGGIYSPSGLDLDAVRAWVA
                     ARRTLAGYPDVQHVSNSELLELPCDILVLAALEEQLTAENAPRIDTKLVVEGANGPTT
                     IEADAILVDRGILILPDVLTNAGGVTVSYFEWVQDLGRLFWTRDEIRARLADKLADAF
                     DRVWALAEQKGISLRQAALVAGINEVAAALKARGIYP"
     gene            8256..8669
                     /locus_tag="VF225_08745"
     CDS             8256..8669
                     /locus_tag="VF225_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7145381.1"
                     /translation="MTSTNQHVRDAMVPEPGALDVTASAQEAGERLSDPAVRAVLVVD
                     DGKFVGVVTRKTLVSQVVAPGLDPRSTTLHEIAEPPLFTLEASMDLDEAFRALEEQDF
                     ERVPVVEDGRLVGILSRTVLQRRLAEDEPPPPLEP"
     gene            8685..9080
                     /locus_tag="VF225_08750"
     CDS             8685..9080
                     /locus_tag="VF225_08750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7145382.1"
                     /translation="MSGLSTLAGEKYISLTTFKQDGTPVATPVWVVSDDGRRLLVWTG
                     PQTWKVKRLRRDPRVIVSASDLRGRTRGESYEGVGRLLDIPQGSLVEPLLDRKYGVTR
                     RLLGMFNGIVRTVTRKQQEPAAYIEIVDA"
     gene            complement(9216..9350)
                     /locus_tag="VF225_08755"
     CDS             complement(9216..9350)
                     /locus_tag="VF225_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145383.1"
                     /translation="MEPVVYREEVLTIMGVLGDIRLELEKIHDLLLGDGGEEEEEENS
                     "
     gene            complement(9405..10370)
                     /locus_tag="VF225_08760"
     CDS             complement(9405..10370)
                     /locus_tag="VF225_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7145384.1"
                     /translation="MSRVAAVDLGTNSTRLLVADVDDGRISDLARETRITRLGEGVDE
                     RRRLLPGPIARVRNVLADFRRTAESLGAERILAIATSAVRDAENGKAFLGEVEWSYGF
                     ATRLLSGHEEALLTFRGVTSERPLEQGTVIVDIGGGSTEFVAGGREGVRWHDSLDIGS
                     GRLTERFLHSDPPTEEELGAGAAAVRALLAGRVPDEIQSDTSSAIGVAGTITSLAGLA
                     LGLEEHDRDRVHGFELSAEALEEQLQRLASVPLEERRRMRPLDPERAPVIVGGAVIAR
                     EVLTFFGLAQLEISERDILDGAALAAAELPEAEEGAAPPGAYTCC"
     gene            10442..10594
                     /locus_tag="VF225_08765"
     CDS             10442..10594
                     /locus_tag="VF225_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145385.1"
                     /translation="MRQQAGGFVYVQTNDADHNEVVVFARRGGGALERLDSYRRDHRR
                     RHARDP"
     gene            10787..10860
                     /locus_tag="VF225_08770"
     tRNA            10787..10860
                     /locus_tag="VF225_08770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10821..10823,aa:Met,seq:cat)
BASE COUNT         1775 a         3653 c         3741 g         1850 t
ORIGIN      
        1 gaaccgcttc accaggtcag ccccgaggag ctcctgctct cggaagccgg acagttcgaa
       61 gactccgcgc ccgctcgcga ggatccgtcc gtccttgtcg cagaccacga acccggtgtt
      121 gggcgcgggg tagtagtcgt ccatcagctc gaacagaaag ccgaagccgc agcggtcgca
      181 ggctaccggg acgttgtcga tctcctggag ccggtcgtgg tcgatcgagc gctgcttgca
      241 gttgggacag atgaagtagg gcacccggtc aaggtagctc cgctagatcc agctacgcct
      301 cctgaagaac acgaactgcg cgatcgtggt gacgacgatc aggccccagg accaggcgta
      361 gccccactgc cagccgagct cggggaagtg gtgcttgaag ttctggccgt agaccccgac
      421 gatgaaagtc ggcaccagga gcatcgacgc gagtgcggtg agcttcttgg tcacctcgtt
      481 ctggtcgttg gcgatcttgg cctgcaggta gtcacggacg ctggcgatga ggtcgcgtga
      541 cagctcgaga ccgtcgaatg cgcggagaag cttgtcgtag gcgctgttga aggcgacctc
      601 gacgtcgtgg gggaacactt cctctccctc ctccacctcg acgacgttgt cgacgacacg
      661 ccggatggcg tcgcgggtcg gggacagtgt gcggcggatg tgcaggaggt cgtggcgaag
      721 ctcgctcaac cggtcgcgcg tctgaggggc aggggctgtc tcgacgccgt cctcgagctc
      781 gtcgatctcc tcgtcgagct gatcgaccag gtcgagatac cgctcggcga tgtcgtcgac
      841 gagccgatag aacatcatcc ccgcgctgtc gtccgggcga cacgactccc gcgctgggcg
      901 cgggtcgtag ggatgctcgc ccggaggcgt cttgctcacg gtgacgagga cgtcgtgtgt
      961 gacgacgacg tcgacctcct ggtagaagac gcgatcctcc tccgggactc caactgcgac
     1021 gaggaagatg ccgaagatgt acccgccgtg cccctgcagc gtcggccgcg gctcgtcggt
     1081 gtgctctgcc ggcgcaagca agcggtcgag cgccgtctcg tacagctccc gcgggagcct
     1141 ctcgcggagc tcctccgggg tcggatcgat caggtcgatc cattcggcca tcggtcgagg
     1201 ctagcggcgg tgtcggctag tgcgagctaa gagcggggtg ctgcttcggc ggccatcaaa
     1261 tgcccttgca gcgcggcgag gtacttctcc gcgtcgagtg cgcccatgca gccggacccg
     1321 gccgcggtaa cggcctgccg gtacacgtga tcctgcacgt cgccgacggc gaagactccg
     1381 ggcacgttgg tctccgtcga atgcgccttg gtgatcagat agccggcttc gtcgtgctcg
     1441 agctggtcga cgaagagcgc cgtgttgggg tcgtggccga tcgccgcgaa gacgccgtcc
     1501 gcgtcgagct cccacgtctc gtcggtgttg acgtcgcgca ggcgcacgcc ggtaacgcgc
     1561 tcgtctccga gcaccgcgtc caccactgcg ttggtgaccc agtcgatctt gtcgttcccg
     1621 cgggcacggt cctccatgat ctgcgaggcc cggaactcgt cgcgccggtg cacgatcgtc
     1681 accttggctg cgaagcgggc gaggaaggtt gcctcctcca tcgcggaatc gccgcctccg
     1741 acgaccacca cgtgcttctg cgggaagaag gcggcgtcgc aggtcgcgca gtacgagacg
     1801 ccgcggccct ggaggcggcg ctcggactcg agcccgagct ggcgggcggt ggccccggtg
     1861 gccacgatca cggtctcggc gcggtactcc tcgtcgccca cccacacccg gtgggggcgc
     1921 tcggtgaagt cgaccttggt cacatcgtcc gtgatgaagt cggtcccgaa ccgctcggcc
     1981 tggcggcgaa actcctgcat catctcgggg cccaggacac cgtcggcgta cccggggtag
     2041 ttctcaacat cgctcgtgat catcagctgg ccgccccagt tgaagccctc gatcacgagc
     2101 ggcctcagat tcgcgcgggc agcgtagagt gcggcggtgt agcccgccgg gccccctccg
     2161 ataatgatca tctcgcgaac gtcgctgtca gtcatagtcc tccaatagtt ccctggttct
     2221 ctttattaaa cgatttcggt cggccgaacc ttccgaggtc gagcggcctt gggggcctct
     2281 cgatgcatag aatccgcgcg cgcatggtac gtaggggcgg gcgatggctg gaacggtgac
     2341 gtggtcgctc ctgcgggagc tggctggctt ccgagcggag aagggctgcg cgatcagcct
     2401 gtgcctgaac ctcgatccat ccgatgtccc gaccgccggg gacgcccaga cgcggatgaa
     2461 ctccatgctg aacgcggctg acaagaccga caggaccgac ctcacccacg agcagcgctc
     2521 cgccctgaag gcggacttcg agcggatcgc gacctggttc gacgacgact tcgagcgcga
     2581 cggctcccag gggctggcgg tcttcgcggc agggctcgac aacttctggc ggacgctccc
     2641 cctgccgcag cctgtccgcg acagtgcgaa ggtcggccgc gatttctacc ttgcgccgct
     2701 cgtcccactc gtgagccgcg gggacgggac gatcgtcgcc gtcgtggggc gtgagcaggg
     2761 gcagctctac cgcctgcgcg cgggccggct cgaggagatc gccgagcact acgacgaaca
     2821 gcccgggcag cacgaccagg gcgggtggtc gcagggtcgg taccaacgcc acatcgagaa
     2881 gctcgtccag gaacacctca agggactcgc gcaggagctc gatcggagca agcgtcggct
     2941 ccagtcgccg aaggtcgtgc tcgtctgttc ggaggagatg cgctcggagt tcaaagacgc
     3001 gctctcggcc gaggtgcgcg cggcgttggt ggggtgggcg cccgcacagg cgcaggccgg
     3061 cccggcggag ctcctccagg ccgtgacacc ggtcctcgag gaggcggagt cgaaggacga
     3121 ggccgaggtg atcgagcgct ggcgcgagga agccggccgc aacgcgcggg ccgcttcagg
     3181 gtgggagcag acgctcgaag ctgcgtcgga tgcccgtgtc gagctgctcc tcttccagga
     3241 aggggccgac cggcctgcgt tccgttgccc cgcgtgcggc cgcgccgcgg tgtcagaggg
     3301 gagctgcccg ctcgacggga cccaactcga gccgaccgac gccgggctcg acctggccgt
     3361 gcaccagaca cttgcccacg gcggcgccgt gtgggcgatc cgccaccacg aggacctcgc
     3421 accggtcgag ggcatcggcg cgctgctgcg cttctagctt gccgtgctag gcagcgtcgg
     3481 cggcgacgcc cggtagctcc agcacggtct cgccctcgag ctcttcctgt tcctcgaccg
     3541 cacgctcgag cttcgtcccg aacttgtcga ggcgcttgcc cgcctcgtgg tacttggact
     3601 gcgcatggcc gagatgcgtg ccgaccttgt cgaagtcgtc ggcgaagcga tcgaagtcgc
     3661 gccggagatc cgccacgtag gccatgacct cgtgcgcgtg ctcctcgatc tgcatcccgc
     3721 gcaggccgag tgcgatcacc tgcaggtagg ccgtgaacgt ggtcggggag acggggaaga
     3781 cgcgccgctc gtgcgcgtac gagagcagcg ctccagtctt gccgcacgcc agctcgtagt
     3841 agaccccttc gaccgggatg tacatgaagg cgaagtcata ggtcccctcg tcggggcgga
     3901 tgtacttgcc cgcgattgcg tcgatgtgct gcttgacgtc gcggccgaac tgccgctctg
     3961 ccagctgccg ctcgtcgtcg gtctcggcgt cgacgagccg gatgtagttg tcgagcggga
     4021 acttcgagtc gacagggatc gtccggtccg cgcgcacgat cgcgtcgacg cgctcgccgg
     4081 agtcgaacgt gtactgcatc tcgtaggccg aaggcggcag ccggtcgcgc agaaggttct
     4141 cgagcaggag ctcgccgaag cccccacgcg ccttcggcgg ccgaagtgcc tgctccagcc
     4201 gcgcgagatc cttcgcccgc tcgagcatct gcgtcgtggc ctcgtcaacc ttgccgaggc
     4261 gctcgtggat cttgttggtg gtctgcgacg cgctctcgag ccggcggtcg acctttgtgt
     4321 ccaactcacc gagccggcgg tccatggtct cggtgactcc gacgagccgt cgatcgacct
     4381 cggcgttccg ctcggacagc tgcgagctcc agtcggttcg caggtctcgg agcgcgcgcg
     4441 ccagccaggc gagggcggcg gccagcccaa cgccgagcag gagaatgagg atcacgtctg
     4501 tcacgagccg acaaacggta gaggggcggt cggacggaac gaagtggggc ggacccaggc
     4561 ccgccccacc gctgtctctc aggtgttctc gcgctccgcg tgggcctctg cggcctggtt
     4621 gcgctgttct tgcgccatct cttcgtgctt ggcggcgcgg tcctctgccg cgccttggcg
     4681 ttcctgcgca gcgccctcgc gtcgcatcga ggcgtcaccc ttcagatctc cggccgcctt
     4741 cttgccacgg ccgagcagcc tgtcgagcag tcccatcttg gcctcccgct tcttgttggg
     4801 aacgcgcttc gaggtcccca gtatgaagcc ctctcggtcg accagccgct cgcgcgccga
     4861 ggcgggccgc gtcccgccat agcgccaggg aaccacgggt tcacccgcgt caaaaaaaac
     4921 gccaaagccg gcgaacccga gggttcatcg gccttggctc gaggagcata ggccgcttga
     4981 acaggcgcag tcaagagccg tctctcgtga aaaaacgcgc acgttgcgaa cttccgccgc
     5041 aggccctcgg gggcgggtac ccttgccgcg ccgtggcgaa gaaagtgcgc acaccacccc
     5101 ctccgaggcg cgttcaggcc cctcagcgcc gcgaaactcg ccgaccccct aggggatccc
     5161 agcgctcgcc ctggatctat gccgtcgggg gcgcactcgc cgccgcggtg atcgccgccg
     5221 cggtcgtggg gatactcgtg gcgagcggcg gaggaagcac gacgaaggcg acggcggcga
     5281 actacaacgc actgcccggg atccgcagga cgaaggcgcc gtggccgccc gagtacctct
     5341 acctcgccga ccgcctcgcc ccgctcggtc tgacgacgct cggcggccac aacgggctcg
     5401 tcctccactt ccacgtgcac atcgacatct tcgtgaacgg caagaaggtc aagatcccgg
     5461 cgctcgtggg catcaatgtc ggtgcgggct ggctgacgga gctgcacacc cacgacgcgc
     5521 gcggggtgat ccatatcgag gcgcagaagt cacgcgactt cacggtcggc cagttctttt
     5581 ccgagtgggc ggtctatctc gactcgcact cgatcggcgg ctattcgggg atgaggtggt
     5641 acctgaacgg caagcttcag accggcaacc cggcgacgct cgtcttcaag ccgcatcagg
     5701 agatcgcgtt cgtcgtcggc aaggcgccgg ccaagattcc ttccagttac gccttcaggg
     5761 cgggtgaata acccagggaa ccgcggctat tctgagcccg ccggggtggc gaaactggtg
     5821 atacgcgccc gacttaaaat cgggtgtccc ttttcagggg cgtaagggtt cgagtccctt
     5881 ccccggcact ccggcgcgaa atccggccgg ccgcgccggc catgatgatt tcgcgccgtt
     5941 cgtctttcga tccggtgcgc ttccggcagc ggcgggctga gcccgccgct gcgtcgagga
     6001 gaacctaggc gagacgccac gaaaacagtc gacgcgagga aaggtcgcaa ccggcgttca
     6061 taggatgaat gcgtggcgat ccgtgaagtg cgggcctcgg gcctcgagta cctgggtctc
     6121 gcgacgcagc tcctgcagcg cgctcgcctc gcagacgcgg aggcaggtct ttgggaagcc
     6181 gctgatcttc agtggtggtg gcggacgcct cggtcctcag acgcaatcga ccagttcttc
     6241 tggatcgatg acgaggggcc ggtcgcaggc ctcgtgctga cggactgggg ccgatcgtgg
     6301 ggttgcgacc tgatcgtcgt ccccggtgtc tcgaccgttc cgctctcgac cgtctgggcg
     6361 cgcgcggtcg aggccatcga cgcctttggg ctcgaagcgg tcgaagtgct cgcccgtgac
     6421 gacgatgtcg agctgcgaaa cctgctgtcc ggcgccggct tcgtgcccga cgacaaccaa
     6481 tcggggatcg cctggatgaa tgcgcaggag cagcccgacg tggccgctct gcccgaagga
     6541 ttcgtgcttg tggaccgtgc gcaagagacg aacacgccac atccgatgcg gcgacgaagc
     6601 ggcgaagagg tcgaagcgcg cctcctgcgg tgctcgctct acgacccggg actggacctc
     6661 gccgtcgagg cctctgacgg ccaggccgcc ggttacgcac tcttctggtt cgaccccgtg
     6721 accaacgtcg gactggtcga gccgatgaga gtcgaggtcg cgtaccagcg gcgcggcctg
     6781 gcccgggcgc tgctcaccgc cggtctcgat cggctggctc ggcgcggggc ccggcgtctg
     6841 aaggtcggct atgccaccga cgtggcacgg cggctctatg tcggcgccgg cttccgcgtc
     6901 acctctacga gcacgtcata cggccggagg ggaccagcgc tgcaccgagt ggaaacctag
     6961 actccgagcg tgaccgagct cgaagagctt tctgccgaag agcagccgct cgaatgggat
     7021 acgccgctct ttcggactgc gctgatgcag ttcgagcagg cgattcccta cgcagaggtc
     7081 aacgaatcga tcgcggagcg gctccgctac ccagagcgtg cggtgatggt gacggttccg
     7141 attcgccgcg acgaagggca cgtcgacgtc tttccggctt atcgcgttca gcattcgagc
     7201 gtgctcggcc cgacgaaggg cggcatccgc tacgacccac acgtcaccct cggcgaatgc
     7261 accgcgctcg cggtctggat gacgtggaag tgcgcgctcc ttcgtctgcc ctacggcggc
     7321 gcgaagggag gcgtccgctg caatccacgt gagctctccg cgcgcgaact ggagcggctg
     7381 acccgtcgct acacgagcga gttgcgcggc gtgatcggac cgcaggaaga catccccgct
     7441 ccggacatgg ccacgaacga gcagacgatg gcgtggatta tggacacgta ctcgatgcag
     7501 gtcggccacg ctgtgccgga gatcgtcacc ggcaagccca tctcgctcgg cgggtcgctc
     7561 ttccggcacg aggcgaccgg agcgggcgtc gtcatggtga tcgaacgcgc ctgccatcga
     7621 ctcggctggg acctcgcgga gcagcgctgt gtcgtgcaag ggttcggcaa cgtcggtggg
     7681 atcgcagcgc aggagctcgt ctcgaaaggc gcggccgtgc tcgccgtctc ggacgtttcc
     7741 ggtgggatct actcgccctc gggactcgat ctcgacgcgg tacgcgcatg ggttgcggcg
     7801 cgcagaacac tcgccggtta tccggacgtc cagcacgtgt cgaactccga gctgctggag
     7861 ctgccgtgcg acatcctcgt cctcgccgcc ctggaagagc agctcactgc ggagaacgct
     7921 ccgcgcatcg acacgaagct cgtcgtggag ggagccaacg gcccgacgac gatcgaagcg
     7981 gatgcgatcc tcgtggaccg aggcatcctg atcttgcccg acgtgctgac gaacgcgggc
     8041 ggcgtgacgg tgtcgtattt cgagtgggtg caggacctcg gccggctctt ctggactcgc
     8101 gacgagattc gcgcgcgtct cgcggacaag ctcgccgatg cgttcgaccg cgtctgggcg
     8161 ctcgctgaac agaaggggat ttcgctacgg caagccgcgc ttgtcgccgg catcaacgag
     8221 gtggccgccg cgctcaaggc gcggggaatc tacccgtgac ttcgaccaac caacacgtcc
     8281 gcgatgcgat ggtgccggag cctggcgcgc tcgacgtgac cgcgagcgcg caggaagcgg
     8341 gcgagcggct gtccgatccc gcggtgcggg cggtgctcgt cgtcgacgac ggcaagttcg
     8401 tcggcgtggt cacgcgcaag acgctcgtct cgcaggtcgt cgcgcccggt ctggatccac
     8461 gcagcacaac gctgcatgag atcgcggagc cgccgctctt cacgcttgag gcgtcgatgg
     8521 acctcgacga ggcatttcgc gcgctggagg agcaggactt cgaacgcgtg ccagtcgtcg
     8581 aagacggcag gcttgtcggg attctgtcgc ggaccgtctt gcagcgccgc ctcgccgaag
     8641 acgagccccc gcccccgtta gagccttagg ggtaccctcg tacgatgagc ggcctgagca
     8701 cgctggccgg cgagaagtac atcagcctga cgaccttcaa gcaggacggc actcccgtgg
     8761 cgacgccggt ctgggtcgtg agcgacgacg ggcgccggtt gctcgtgtgg acggggccgc
     8821 agacctggaa ggtcaagcgg ctgcggcgtg atcctcgcgt gattgtctcg gcttctgact
     8881 tacgtggccg gacccgcggc gagtcctacg agggagtggg gcggttgctc gacatcccgc
     8941 agggaagcct cgtcgagccg ctgctcgacc ggaagtacgg cgtgacgcgt cggctactcg
     9001 ggatgttcaa cggcattgtg cgaaccgtga cccgaaagca gcaggagccg gctgcgtaca
     9061 tcgagatcgt cgacgcctga aatcgctaag agtcggtcgc tggttctcga cggcggcgct
     9121 cttccaggtc tgcccagccg cgatcgacga gctcgcgaag tcggcgaagg ttcgcatccg
     9181 attctgcgcg ccattccctc tctctggcgg cctgttcagg agttctcttc ttcctcttct
     9241 tcaccgccgt cccctagcag caaatcgtgg atcttctcca gctccaggcg aatatcgccc
     9301 agtacgccca tgatagtcag cacttcttcg cgatagacaa cgggctccac cggtcaaccg
     9361 tcacgcagcg gcaactcgct cgcaagacgc ccttccagac aggtctaaca gcaggtgtaa
     9421 gcgccgggag gcgccgcgcc ctcttccgct tccggcagtt ccgcagccgc aagagcggcg
     9481 ccgtcgagga tgtcgcgctc gctgatctcc agctgagcga ggccgaaaaa cgtgagtacc
     9541 tcgcgagcta tgaccgcgcc acccacgatc acgggtgcgc gctcaggatc gagggggcgc
     9601 atccgacgtc gctcctcgag cggcacagag gcgagacgct gaagctgctc ttcgagcgcc
     9661 tcagcactca actcgaatcc atgtacgcgg tcgcggtcgt gctcctccag gccgagcgcc
     9721 agtccggcga ggctcgtgat cgtccctgcg acgccgatcg cggagctcgt gtcgctttga
     9781 atctcgtccg ggacgcgccc ggcgagaagc gcgcgcaccg cggctgctcc cgcgccaagc
     9841 tcctcttccg tcggcggatc cgagtgcagg aacctctcgg tgagacggcc ggagccgatg
     9901 tcgagactgt cgtgccagcg aacgccctcc cgtccgccgg cgacgaactc ggtcgagccg
     9961 ccgccgatgt caacgatcac ggtgccttgc tcgagcggcc gctccgacgt gacaccgcgg
    10021 aacgtcagca acgcttcttc gtgtcctgag agcaagcggg tcgcgaagcc gtagctccac
    10081 tcgacctcgc cgaggaacgc cttgccgttt tccgcgtcgc gaactgcgct cgtcgcgatc
    10141 gcgagtattc gttctgcccc gagtgattcc gccgtgcgtc gaaagtcggc gaggacgttc
    10201 cgcactcggg cgatcgggcc ggggaggagg cgtctgcgct cgtcgacgcc ctcgccgagc
    10261 cgggtgatgc gtgtctcgcg cgcgagatcg gagatgcgcc cgtcgtcgac gtccgcgacg
    10321 aggagacgcg tcgagttcgt gcccagatcg accgcggcga cacggctcat cgttcgactg
    10381 taacgacccg ctctctcgag gggcggtagc tggacatcgg gcgacgaaag ggatattcca
    10441 aatgaggcag caagcaggcg gattcgtcta tgtgcaaacg aacgacgcag accacaacga
    10501 ggtcgtcgtg ttcgcgcgcc gcggcggcgg cgcactcgaa cgcctcgatt cctatcgccg
    10561 cgaccaccgt cgacggcacg cccgggatcc gtgacgaggc gttcagctgg acggggagta
    10621 cctgtatgcg ctacacgccg acacgggccg ggtgttcggc tggaaggtcg gcgccggcgg
    10681 agaactggcc gcagtcggct cggcgaacgg cctcccgctg acggctgccg ggctggcggc
    10741 gagttagcca gtctcacgcc tcgcgagcta taatcgagac aagcgacgcg gggtggagca
    10801 gtcaggtagc tcgccgggct cataacccgg aggtcgcagg ttcaaatcct gcccccgcta
    10861 cttaaaaggc tctggaaaca gggccttttg tttgcaagtg agcgccaatc tgccgagcca
    10921 gaacggagag agcagaccac cgctgcaccg tcctgccgtt acgcgccgac ggcgatgcga
    10981 taggcggcga ccgggtgacc gttcagttct cgggcgtaa
//
LOCUS       DASVEU010000207         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1502814,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000207 DASVEU010000000
VERSION     DASVEU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502814"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..751)
                     /locus_tag="VF225_08775"
     CDS             complement(83..751)
                     /locus_tag="VF225_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145386.1"
                     /translation="MKPKTLDHVAFWVADREPIVDFLTQHVGMHVIDQQDAFTLVGSD
                     ARRGKLTLFTAEGPRQQGAFKHVALRVSDLNRARVGLPDGVEVELGEGVRVRLVEAPT
                     DVEYDLDHVALYSVDPEQTAQEYTTLGFNAAERSSEGHPRVEVGGAYVEFHPGDPGGA
                     EKPLLNHVAVLVDEVEPVIAEAEERDIVDNVVDAANTRAVFVWGPERVRIEYVEHKPG
                     FALT"
     gene            complement(792..947)
                     /locus_tag="VF225_08780"
     CDS             complement(792..947)
                     /locus_tag="VF225_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145387.1"
                     /translation="MPQGAWSDKRERQYEHIKASEKKQGRSTKRAKEIAAATVNKQRA
                     ESGETKK"
     gene            complement(1002..1307)
                     /locus_tag="VF225_08785"
     CDS             complement(1002..1307)
                     /locus_tag="VF225_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX7145388.1"
                     /translation="MTYIIAEPCIDIKDKSCVDVCPVDCIHEAERILVIDPEECIDCG
                     ACEPECPVEAIFPEDALPDKWQPFVKINYAYGEGFDVVNKLTDEYATEHDVQNPPLE"
     gene            complement(1327..1632)
                     /locus_tag="VF225_08790"
     CDS             complement(1327..1632)
                     /locus_tag="VF225_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145389.1"
                     /translation="MATILLCGVDVFFRGKLEALLPGHHFVTTDSADWPDLVIADISR
                     VDPMDVADWYPEIPILGFGGHTDTAGLRRAHEAGFDQVLVKNALQERAAQVVDELIA"
     gene            1692..2000
                     /locus_tag="VF225_08795"
     CDS             1692..2000
                     /locus_tag="VF225_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7145390.1"
                     /translation="MEDGFITVARVDDVPAGTIHSVKAGDEEIALAHCNGGFYAVQPH
                     CLHLHGPLGQGELEGCVLTCPWHGWQYDVRTGQNEFDLAIQLRTYDVQVEDGEVRVRV
                     "
     gene            2110..2304
                     /locus_tag="VF225_08800"
     CDS             2110..2304
                     /locus_tag="VF225_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145391.1"
                     /translation="MHVAVAWYQAGRGRKIGTERQLAKAVRRLEPFAPTHRGIELEPL
                     LAQLRELHEQGSLELPPPLV"
     gene            complement(2301..3008)
                     /locus_tag="VF225_08805"
     CDS             complement(2301..3008)
                     /locus_tag="VF225_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7145392.1"
                     /translation="MEKAGGLFLLGFASIAFPCVLPLVPGYLSAVSAVEAQRLGDPGV
                     GRRVALASLPFIAGFAIVFVALGAAANLIAGVLNDQTRQALAGFILVVAGLAFMGLLP
                     GTDRLVAGGLIQGARKSGSRVLLGGAFAICAAPCVGPFLAEAFAAAGNSETVIRGSAW
                     LAAYSAGLGAAFLLVAVFFVRGMTLFRWMRDHYRVFSIVGGTILVLLGLLLFFDEFYR
                     VQVYTHRLLEAIGLGDL"
BASE COUNT          554 a         1090 c         1010 g          495 t
ORIGIN      
        1 ttctcgtaca cgacaggggt cgcgccgagc tcacgtgcgc gcgcggcggc ggacagtccc
       61 gccatccccg cccccgcgat gactacgtga gagcgaatcc cggcttgtgc tcgacgtact
      121 cgatgcgcac gcgctccggc ccccagacga acactgcacg cgtgttcgct gcgtcgacga
      181 cgttgtcgac gatgtcacgc tcctcagcct cggcgatgac cggctcgacc tcgtcgacga
      241 gcacggcgac gtggttcagc aacggcttct cggcgccgcc tggatcgccg ggatggaact
      301 cgacgtacgc gccgccgacc tcgacgcgcg gatgaccctc actggatcgc tcggcggcat
      361 tgaaaccaag cgtcgtgtac tcctgcgccg tttgctcggg gtcgaccgaa tacagcgcga
      421 cgtggtcgag gtcgtactcg acgtcggtcg gcgcctcgac gaggcgcacc cgtacgcctt
      481 cgccgagctc gacctccaca ccgtccggaa gtccgacgcg cgcacgattg agatccgaca
      541 cgcgcagcgc gacgtgcttg aacgcaccct gctggcgcgg tccttcggcc gtgaacagcg
      601 tcagcttccc gcgccgcgca tcggagccga ccagcgtgaa cgcatcctgc tgatcgatga
      661 cgtgcatccc gacatgctgc gtcagaaaat cgacgatcgg ctcgcgatcc gcgacccaga
      721 aggcgacgtg atccagggtc tttggcttca cgtcgccagc gtacctacgg ggtaccgctc
      781 tgccgagcga gtcacttctt ggtctcgccg gactccgctc gctgcttgtt gacggtggcc
      841 gctgcgatct ccttggcccg cttcgtcgag cggccctgct tcttctccga cgccttgatg
      901 tgctcgtact gacgttcgcg cttatcgctc catgctccct gtggcatgtg acaaaccccc
      961 tctggtagac gacacatccc gcctacccac ttccctgagg gttattccag gggtggattc
     1021 tggacgtcgt gctctgtggc gtactcgtcg gtcagcttgt tcacgacgtc gaagccctcc
     1081 ccgtatgcat agttgatctt cacgaacggc tgccatttgt ccggtagcgc atcctccgga
     1141 aagatcgcct ccaccgggca ttccggctcg cacgcgccgc aatcgatgca ctcctcggga
     1201 tcgatcacca ggatgcgttc ggcttcatgg atgcagtcga ccgggcagac gtccacgcag
     1261 gacttgtctt tgatgtcgat gcagggctcg gcgatgatgt aggtcactgg gggcgaccta
     1321 ctttactcaa gcgatcagct catcaacgac ctgagccgcg cgctcctgaa gcgcgttctt
     1381 cacgagcacc tggtcgaagc ccgcctcgtg agcgcgccgc agcccggccg tgtcggtgtg
     1441 ccctccgaag ccgaggatcg gtatctccgg ataccagtcg gcgacatcca tcggatcgac
     1501 gcggctgatg tccgcgatca cgagatcggg ccagtccgcg ctgtcggtcg tgacgaagtg
     1561 atgtccaggg agcaaggcct cgagcttgcc ccgaaagaag acatcgaccc cacagagcag
     1621 gatcgtcgcc acgacgagag ggtatcgcgc tcgggcgtgc ccactcaagc ccttgatata
     1681 gggtcgcacg ggtggaagat ggcttcatca ccgtcgctcg agtcgacgat gttcccgccg
     1741 gaaccatcca cagcgtcaag gccggcgacg aggagatcgc cttggcccat tgcaacggcg
     1801 gcttctatgc cgtgcaaccg cactgcctcc acctccacgg gcccctcggc cagggcgagc
     1861 tggagggctg cgtgctcacg tgcccctggc acgggtggca gtacgacgtt cgcaccggcc
     1921 agaacgagtt cgacctcgcg atccagctcc gcacgtacga cgtccaggtg gaagacggag
     1981 aagttcgagt ccgcgtatga gacgggcgtc gagctgattc gtgcaggcga gtacttcgcc
     2041 gcacatgagg agctcgagga tgcctggcgc gcggcgccca ccggggagcg cgacttcttt
     2101 cagggcctgg tgcacgtcgc cgtggcgtgg taccaggccg gacgcggacg caagatcggc
     2161 accgagcggc aactggcaaa agcggtgagg cgcctcgagc cgttcgcgcc cacgcaccgc
     2221 gggatcgagc tcgaaccgct gctcgcgcag ctgcgggagc tgcacgagca gggctcgctc
     2281 gagctcccac cgccactcgt ctagagatcg ccgaggccga tggcctcgag caggcgatgc
     2341 gtgtagacct gcacgcggta gaactcgtcg aagaacagca gcaggccgag caggacgagg
     2401 atggttccgc cgacgatcga gaatacgcgg tagtggtcgc gcatccaacg gaacagcgtc
     2461 atgcccctca cgaagaagac ggcaacgagg aggaatgcgg cgccgaggcc ggccgaatag
     2521 gcggcgagcc aggcagagcc gcgaatgacc gtttccgagt tgccggccgc cgcgaacgcc
     2581 tccgcgagga acggcccgac gcagggcgcg gcgcagatcg caaaagcgcc gccgagcagt
     2641 acgcgcgaac cgctcttccg cgcaccctgg atcagcccgc ccgcaaccag ccggtccgtc
     2701 cccggcagca gtcccatgaa ggccagtccg gcgacaacga ggatgaagcc ggccagcgcc
     2761 tgcctcgtct gatcgttcag cactcccgcg atcaggttcg ccgctgcgcc gagagccacg
     2821 aacacgatcg cgaagccggc aatgaacgga aggctcgcga gtgcgacgcg ccgaccgacg
     2881 ccggggtcgc cgagccgctg cgcctcgacc gccgagaccg ccgagaggta acccggcacg
     2941 agcggcaata cgcacgggaa ggcaatcgag gcgaacccga gcaggaacag tccgccggcc
     3001 ttttccatca gccctcgaag cggcgcagct cgtcgtcgag ccgccgctct gacgccgtgt
     3061 cgagcggcgg gccggcgggc gccggctcgc gcgcgcgggc ccaccgccgc gcgcccacac
     3121 cgactgcgac cgcggcggcc agaatgccc
//
LOCUS       DASVEU010000208         9750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_535144,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000208 DASVEU010000000
VERSION     DASVEU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535144"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1463
                     /locus_tag="VF225_08810"
     CDS             135..1463
                     /locus_tag="VF225_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145393.1"
                     /translation="MGIVLLVALVLGAALIAATARTPASAGTSGAKADNITIWVGWSA
                     AELKAFKGVVAEYDKKHSDVTVKVVGGINDDKITAAIRSGKVPDVVSSFTSANVGQYC
                     NSGAWLDLKPFLSKDKIDVNMFPKTSRYYTQYKGKRCALPLLADVYGFYYNKKMFKAA
                     GLTSPPKTMSQLTAYAKKLTKKNPDGSIKVAGYNPFLGFYAGNAPDLSAYAPLFGAKY
                     VDASGGSVLSRDPAWSKMMKWQKSLVDWYGYKKLQRFNAGAADEFSASHAFEIGKLAM
                     MIDGEWRVAFIGREHPSLDYGTAPAPVDDAHPELYGAGYVNGTIIGIPKGVKHKDAAW
                     ALVKYLTTDSHALAIFSNGVRNVPTTTASLTSPDIKPDSHFKTFLKIFANSKSTTTPI
                     TAAGNAYGSLIQAFAVKWQAGHGGDLAKGLQKLDKQIDAQLKQAQAGGVP"
     gene            1559..2455
                     /locus_tag="VF225_08815"
     CDS             1559..2455
                     /locus_tag="VF225_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7145394.1"
                     /translation="MAPWLIGFSVFFGYPLVMSVYLSFTHYDLLSSPKWIGTANYRYL
                     FTQDPQVGQAVKNTLWIIAVMVPLQVVFAFGIAMMLARARRGAGFFRTIFYLPALAPP
                     VAATLAFVYILNPATGPVNILLKHLGIEGPLWFQSPGWSKPSLVMLGLWGVGNIMIIF
                     LAALLDVPRHLYESAELDGAGPLQRLRWVTLPTISPVILFSVVIGVIAGLQYFTQAYV
                     AANVASGQASQAGDQTVNNLGYPEASTLFYPVLLYQHGFRYFNMGYASAMSVLLLVVA
                     FTITVLIIRNSRRWVHYVGAAK"
     gene            2452..3339
                     /locus_tag="VF225_08820"
     CDS             2452..3339
                     /locus_tag="VF225_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7145395.1"
                     /translation="MSTETLPVAAALTRRRPPASVRRHRFLLAVANHSLLIVAAIAFL
                     APFVFIFLTALMTNGQALSSHLWPSPFRWQNFVDVFHQAPIWRWALNTMIYAVLATLG
                     VLVSSIPVAYALSRIRWRGRNFAFGLVLIALMLPPVVAVVPLYVMWAKLGLVGGLAPL
                     IIPSWFGDAFSIFLLRQFFLTIPEEYLDAARVDGCGELRILATVVLRLAKPAIAAVAL
                     FSFLYTFNDFFLPLLYTRENPSSWVLQIGLSEFRSLHQVQWNLTMAATLLVMAPVIIV
                     FFLAQKAFVEGVTLTGVKG"
     gene            3479..4753
                     /locus_tag="VF225_08825"
     CDS             3479..4753
                     /locus_tag="VF225_08825"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEX7145396.1"
                     /translation="MKIAVIGGGSTYTPELVSGLSRERDRIDVSELVLQDIDPERREV
                     VGGLAQRMLDRQGYGGRLEITDDLDRAVDGADFVLIQIRVGGQDARLSDETVPLACGC
                     IGQETTGAGGLGKALRTVPVVLGIADRVRQCAADGAWIVDFTNPVGIVTRSLLDHGHR
                     AVGLCNVAIGFQRSFARMLGVEPDRVRVDQVGLNHLSWVRHVWLDGEDVIADLMAAHG
                     DEIARQSGLSRQLLDELGVIPSYYLHYFYSHDAVLAEQLGGVPRATTVAEIERELLDL
                     YRDPNLVEKPSLLEQRGGAFYSEAAIGLVGALAADTGEPHVVDVRNNGTVEGLADDDV
                     IEVLAEVGAGGAKPLPQPPVAPELLGLMQHVTAYERLAAQASVGGDPGIARKALLTHP
                     LIGQHALSEELVDRLLEAGAAHLPQFHAEIGK"
     gene            4750..5766
                     /locus_tag="VF225_08830"
     CDS             4750..5766
                     /locus_tag="VF225_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7145397.1"
                     /translation="MTRAGVLAVDGGNSKTDVALVNADGEVLALVRGALSSPQHLGVD
                     GALAVVEGLLEEALREAQLGDGTGRVAHVVTLLMAGVDFPAEEDAVRVAVEQRGWAQS
                     VHVGNDTFAVLRAGTELGWGVAVTCGAGINCVGVGPDGRHARFPALGAITGDWGGGYD
                     LGLAAVSAAARSEDGRGPRTTLERAVPAHFGVTTPSELAEGIHTGRIDQRRVIELAPL
                     VLAEAADDAVAAEIVQRLASEVVALARVALTRLELTEEPVEVLLGGGVLQDVDGDLLA
                     AIDSGLREAAPNVTVRPTASAAIVGAALLGLDELEAGPEAQARLRRELGAAFSQLEGV
                     ATVG"
     gene            5768..6832
                     /locus_tag="VF225_08835"
     CDS             5768..6832
                     /locus_tag="VF225_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145398.1"
                     /translation="MRYVQATRLYPGTDAPAVDALDLDVHDGEFMVLVGPSGSGKTTA
                     LRMLAGLEEVDAGAVYIGDRDVSDVPPKERDVSMVFQNYALYPYLTVAANIGFPLRIA
                     KVKKAEREKRVREVAKLLGLSDYLERKPGQLSGGQRQRVAMGRAIVRVPSVFLMDEPL
                     SNLDAKLRVQMRADIAALQADFGITTVYVTHDQAEAMTLGHRVAVLHEGHLQQCGPPR
                     HLYERPANVFVAGFIGSPAMNLCTVPVSNGTASFGGVNVPLPEGVVANGRTSLVIGVR
                     PESLDVAEEGIPAKVDVVEDVGADAFVFCSTELGGETMKLVARSEARKAPKQGDRVTL
                     RPRADEAHLFDPANGERLER"
     gene            6832..7902
                     /locus_tag="VF225_08840"
     CDS             6832..7902
                     /locus_tag="VF225_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7145399.1"
                     /translation="MPRVPGPHNPGELFLGEIREQPQALLRLLDEEADFDAVSKVMRE
                     RQPSVVRLVGHGSSDAAASFGVYAFGLLPGWTAMRDSISLTVYYDAKIDLSSSVVIGL
                     SQSGETPDVVEYVKRARGRGAFTVGITNGADSSLAAAADATLALSAGPEHAVAASRTF
                     FNTLAALAWVAGGAAGRGREIAEGLRTVSGQLSESLTLLEQAVSNVAMAFAYVGRMYV
                     IGRGIEFGTAREVALKLTETCRVAAEPLTATDLAHGPIAALDSMFPVWTIASRDESLP
                     AVLEAAARVIEAGATVVASGNAAAEIEGAAYVLPVPEAPHPILSPLLSVVPGQLFAGA
                     LAHAKGLDADYPVGLSKVTRAR"
     gene            8003..8761
                     /locus_tag="VF225_08845"
     CDS             8003..8761
                     /locus_tag="VF225_08845"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145400.1"
                     /translation="MTIFETSQAASASPTVPPFSTAEMRSYPLGTLVYRSGLMPLETV
                     NRALAEAAESGRRLGEVLLDYGLPERDLTRLLAAQNGQEFVDLTEFPIDPEVARLLPQ
                     PVAEMYCALPLQREGDCTLVAVPDADNASHLTRLQAALHGPIRLVTAARSDIKDAIER
                     LHIEPQGMITQMALAEARTQAPPEPTTTYRIEVTLASGATLLVAEATDFEYAKQLGMR
                     VIDEAEAGGTVAIGDATIDGRQVVSVDVLGRNGA"
     gene            8758..9009
                     /locus_tag="VF225_08850"
     CDS             8758..9009
                     /locus_tag="VF225_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145401.1"
                     /translation="MNFPVNAVRELISAVRKDGRPVVRLTCADYGTEWVVAADVYPVD
                     ELTVKPKSAGPYVFDTAQEARSFIESSLVALQLLGCEVA"
     gene            complement(9013..>9750)
                     /gene="rsgA"
                     /locus_tag="VF225_08855"
     CDS             complement(9013..>9750)
                     /gene="rsgA"
                     /locus_tag="VF225_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7145402.1"
                     /translation="QVLAANVDAAFLLAGLDDDFSLRRLERYITIAWESGAQPIVVLT
                     KADLSPDVPEAVLAVETVAIGVPVHPVSNVTGEGLEALDPYLQPGRTIVLLGSSGVGK
                     STLLNRLADEEVMLTRAVAADGTGRHTTTHRELVRLPGGGLVIDTPGLRELRFWEGDL
                     SAAFEDIEALALECKFRNCAHMREPECAVLAAVDDGSLELDRLRSWRKLQRELEAIAA
                     RTDTRLRIARKKRWKQVASVARQKSRP"
BASE COUNT         1555 a         3166 c         3366 g         1663 t
ORIGIN      
        1 gttcggtctc cgtcatagag tcctcgttcg gaacctttac gagattccag attgtcggcg
       61 cactccgcgg cggcgctgga tgaggagatc ggggtcgacg acaatcaccc caagggaggc
      121 agggtatgag acgcgtgggg attgtgttgc tcgtggcgct cgtgctcggc gcggcgctca
      181 tcgcggcaac cgcgagaacg cctgccagcg ccggcacgtc cggcgcgaag gccgacaaca
      241 tcacaatctg ggttggctgg agcgctgctg agctcaaggc cttcaagggc gtggtggcgg
      301 aatacgacaa gaagcactcc gacgtcacgg tgaaagtcgt cggcgggatc aacgacgaca
      361 agatcaccgc ggcgattcgg agtggcaagg tgccggacgt ggtcagctcc ttcacgtcgg
      421 cgaacgtcgg ccagtactgc aactcgggtg cctggctcga cctgaaaccg ttcctgtcga
      481 aggacaagat cgacgtaaac atgttcccga agacatcgcg gtactacacc cagtacaagg
      541 gcaagcgatg tgcgttgccg ttgctcgcgg acgtctacgg cttctactac aacaagaaga
      601 tgttcaaggc ggcggggctg acgagcccgc cgaagacgat gtcgcagttg acggcgtacg
      661 cgaagaagct gacgaagaag aatcccgacg gctcgatcaa ggtcgccggg tacaacccat
      721 tcctcggctt ctacgccggc aacgctcccg acctgagcgc gtacgcgccg ctgttcggcg
      781 ccaagtacgt tgacgcgagc ggcgggtcgg tgctctcccg ggatccggcc tggtcgaaga
      841 tgatgaagtg gcaaaagagc ctcgtcgact ggtacgggta caagaagctc cagcggttca
      901 acgccggagc tgccgacgag ttctcggcct cgcacgcgtt cgagatcggc aaactcgcga
      961 tgatgatcga cggcgagtgg cgtgtggcgt tcatcgggcg tgagcatccg agcctcgact
     1021 acggcacggc gccggcaccg gtcgacgacg ctcatcccga gctctatggg gcgggttatg
     1081 tcaacgggac gatcatcggc atcccgaagg gcgtgaagca caaggacgcg gcgtgggccc
     1141 tggtcaagta cctgacgacc gacagccacg ccttggcgat cttctcgaac ggcgtccgca
     1201 acgtcccgac caccacggcg tcgctgacgt cgccggacat caagccggac tcgcatttca
     1261 agacgttcct gaagatcttc gcgaactcga agtcgacaac gacgccgatc accgcggccg
     1321 gcaatgcgta cggctcgctg atccaggcgt tcgcggtcaa gtggcaggcc gggcatggcg
     1381 gcgacctcgc gaaggggctg cagaagctcg acaagcagat cgacgcacag ctgaagcagg
     1441 cgcaggcggg cggagtgccg taatggcgag cgccgcaacg gcggctgtaa ggccgttgga
     1501 ccggacgcgg gcgaggcgga aggcggcatg gcgccgtcgc cgcctcgtcc tgctgctgat
     1561 ggccccctgg ctgattggct tctcggtctt cttcggctac ccgctcgtga tgagcgtgta
     1621 tctgtcgttc acccactacg acctgttgtc gtcgccgaag tggatcggca cggcgaacta
     1681 ccggtatctc ttcactcagg atccgcaggt gggacaggcg gtgaagaaca ccctctggat
     1741 catcgcggtg atggttccgc tgcaggtggt gttcgcgttc ggcattgcga tgatgctcgc
     1801 gcgtgcgcgc cgtggcgccg gcttcttccg gacgatcttc tacctgccgg cgctggcgcc
     1861 gccggtcgcg gccacgcttg cgttcgtcta catcctcaac ccggcgacag gcccggtgaa
     1921 catcctgctc aagcacctcg gaatcgaggg gccgctgtgg ttccagtcgc ccgggtggtc
     1981 gaagccctcg ctcgtgatgc tcggactgtg gggagtcggg aacatcatga tcatctttct
     2041 cgcggccctc ctcgacgtgc ccaggcacct gtacgagtcg gccgaactgg acggcgcggg
     2101 gccgttgcaa cggctgcgct gggtgacgtt gccgacgatc agtcccgtga tcctcttctc
     2161 ggtcgtgatc ggggtcattg ccgggctcca gtacttcacg caggcctacg tcgccgccaa
     2221 cgtcgcctcc ggccaggcct cgcaggcggg cgaccagacg gtgaacaacc ttggctaccc
     2281 cgaagcgtcg acgctcttct atcccgtcct gctctatcag cacgggttcc gttatttcaa
     2341 catgggctat gcctcggcga tgtcggtgct gctgctcgtc gtcgcgttca ccatcaccgt
     2401 cctcatcatc cgcaactcgc gccgctgggt tcactacgtc ggggcggcga agtgagcacg
     2461 gagaccttgc ctgtcgcggc cgctctgacg aggcgaaggc cgcccgccag cgttcggcgg
     2521 caccggttcc tgctggccgt cgcgaaccac tcgttgctga tcgtggcggc gatcgccttt
     2581 ctcgcaccgt tcgtattcat ctttctgacg gcgctgatga cgaacggaca ggcgctgtcg
     2641 tcgcatctct ggccgagccc gtttcgctgg cagaacttcg tggacgtgtt tcaccaggcg
     2701 ccgatctggc gctgggcctt gaacacgatg atctacgcgg tgctcgccac gctcggcgtg
     2761 ctcgtctcga gcattccggt tgcctacgct ctctcgcgca ttcgctggcg tggccgaaac
     2821 ttcgcgttcg ggctcgtcct gatcgctttg atgctgccgc cggtcgtcgc cgtcgtgccc
     2881 ctctatgtga tgtgggcgaa gctgggcctc gtcgggggcc tcgcgccgct gatcatcccc
     2941 agttggttcg gtgacgcatt ctcgatcttc ctcctgcgcc agttcttcct gacgattccg
     3001 gaggagtacc tcgacgcggc ccgtgtcgac ggctgcggcg agctgcgcat cctcgccacg
     3061 gtcgtcctca ggctcgcgaa gccggcgatc gcggcggttg cactcttctc gttcctctac
     3121 accttcaacg acttcttcct gccgcttctc tatacacgcg agaacccgag cagctgggtg
     3181 ctccagatcg ggctctcgga gttccgctcc ctgcaccagg tgcaatggaa cctcaccatg
     3241 gcggcgaccc tgctcgtgat ggcgccggtg atcatcgtct tcttcctcgc gcagaaggcc
     3301 ttcgtcgaag gcgtgacgct gacgggggta aagggctagt gcccctcccg gcaatacaaa
     3361 acggccgcac cggcgaagcc ggcacggcct cctacggtcg gcatcgcaag cgatgcctcg
     3421 gtgccggctt ctggcccgcg atcacgcggc gccaggatga aatcaccggg aggggcactt
     3481 gaagatcgcc gtgatcggag gcggctcgac ctacacgccg gagctcgtgt cggggttgtc
     3541 gcgcgagcga gatcgcatcg acgtgagcga gctcgtgctg caggacatcg atccggagcg
     3601 gcgcgaggtc gtgggtggac tcgcgcagcg gatgctcgac cggcaggggt acggagggcg
     3661 gctggagatc accgacgatc tcgaccgggc cgtcgacggc gccgacttcg tcctgatcca
     3721 gatccgcgtc ggcggccagg acgctcgcct ttcggacgag accgtgccgc ttgcctgcgg
     3781 ctgcatcggg caggagacca caggggccgg cgggctcggc aaggccctgc gcaccgtccc
     3841 cgtcgtgctc gggatcgccg accgcgtacg acaatgtgca gcggacgggg cctggatcgt
     3901 cgacttcacg aatccggtcg ggatcgtcac acggtcgctg ctcgatcacg ggcatcgggc
     3961 ggtcggtctc tgcaacgtcg cgatcggctt tcagcggagc tttgcgcgga tgctcggcgt
     4021 cgagccggac cgcgtgcggg tcgatcaagt cggactcaac catctctcgt gggttcgtca
     4081 cgtgtggctc gacggtgagg acgtgattgc ggatctcatg gcggcgcacg gcgacgagat
     4141 cgcacggcag agcgggctgt cacggcagct gctggacgag ctcggcgtca ttccctcgta
     4201 ctacctccac tacttctatt cccatgacgc cgtgctcgcg gaacagctcg gcggagtgcc
     4261 ccgcgcgacg acggtcgccg agatcgagcg cgagctgctc gatctgtacc gcgaccccaa
     4321 cctggtcgag aagccgtcgc tcctcgagca gcgtggcggc gcgttctaca gcgaggccgc
     4381 gatcgggctc gtcggcgcgc tcgccgcaga caccggggag cctcacgtcg tcgacgtccg
     4441 caacaacggg accgtcgagg ggcttgccga cgacgacgtg atcgaagtgc tggccgaggt
     4501 cggtgccggc ggcgccaagc cgttgccgca gccaccggtc gcgccggagc tgctcggcct
     4561 catgcagcac gtcaccgcct acgagcggct cgccgcgcag gcgtccgtcg gcggcgaccc
     4621 ggggattgcg cgcaaggcgt tactgacgca cccgctgatc ggccagcatg ctctttcgga
     4681 ggagctcgtc gaccggttgc tcgaagccgg cgccgcacac ctgccgcagt tccacgcgga
     4741 gatcggaaag tgactcgagc tggtgtcctg gccgtcgacg gcggcaactc caagacggac
     4801 gttgcgctcg tcaacgccga cggcgaggtg ctcgcgctcg ttcgcggcgc gctgagctcg
     4861 cctcagcacc tcggcgtcga cggagctctc gccgtggtgg aagggctcct ggaagaggcg
     4921 ctgcgcgaag cccagctggg tgacggcacg ggccgggtcg cccacgtggt gaccctgttg
     4981 atggccggag tcgactttcc tgcggaagaa gacgcggtgc gagtcgccgt cgagcagcgc
     5041 ggctgggctc agagcgtgca cgtgggcaac gacacgtttg cagtcctgcg cgccggcacg
     5101 gaactcgggt ggggcgtcgc ggtcacgtgc ggcgcgggga tcaactgcgt gggtgtcggg
     5161 ccggatggac ggcatgcacg ctttccggcg ctgggcgcga tcaccggaga ctggggcggg
     5221 ggctacgacc tgggcttggc ggccgtctcc gccgcggcgc ggagcgaaga cgggcgcggt
     5281 ccacggacga ccctcgagcg ggccgtcccc gcgcacttcg gcgtcaccac gccgagcgag
     5341 ctcgccgaag ggattcacac ggggcggatc gaccagcgcc gcgtcatcga gctcgcaccg
     5401 ctcgtgctgg cggaggcagc ggacgacgcc gtcgcggccg agatcgtcca gcgcctcgcc
     5461 tccgaggtgg tcgcgctcgc tcgcgtcgcg ctgacgcgat tggagctcac agaggagccc
     5521 gtggaggtgc tgctcggcgg cggtgtcttg caagacgtcg acggcgatct gctggccgct
     5581 atcgattccg gcctgcgcga ggccgctccg aacgtcaccg tccggccgac cgcctccgcg
     5641 gcaatcgtgg gggcggcgct gctcgggctc gacgagctcg aggcgggccc cgaggcccag
     5701 gcacggctgc gccgcgagct cggggcggcg ttttcccagc tggaaggggt ggcgaccgtt
     5761 ggctgaggtg cgatacgtcc aggcgaccag gctctacccc ggaaccgatg cgcctgcggt
     5821 cgacgctctc gacctcgacg tgcacgacgg ggagttcatg gtcctcgtgg gaccgtcggg
     5881 ctcgggcaag acgacagcgc tccggatgct cgccggtctg gaagaggtcg atgcgggcgc
     5941 ggtctatatc ggcgatcgcg acgtcagcga tgtgccgccg aaggagcgcg acgtctcgat
     6001 ggtcttccag aactacgcgc tctacccata tctcaccgtc gcggcgaaca ttggcttccc
     6061 cttacggatc gcgaaggtga aaaaggccga gcgcgaaaag cgggtgaggg aggtcgcgaa
     6121 gctcctcggc ctctccgatt acctcgagcg gaaaccgggc cagctttcgg gcggccagcg
     6181 ccagagggtc gccatgggcc gggcgatcgt ccgagtcccc agcgtgttcc tgatggacga
     6241 gcccctgtcg aacctcgacg cgaagctgcg cgtgcagatg cgggccgaca tcgcagctct
     6301 gcaggccgac ttcggcatca ccaccgttta cgtgacgcac gaccaggccg aggcgatgac
     6361 gctggggcac cgcgtggctg ttctccacga ggggcatctg cagcagtgcg gacccccgcg
     6421 gcatctctac gagaggccgg cgaacgtgtt cgtggccggc ttcatcgggt caccggcgat
     6481 gaacctctgc acggtgccgg tgtccaacgg cacggcctcc ttcggcggcg tgaacgtgcc
     6541 cctgccggag ggcgtggtgg ccaacgggag aacgagcctt gtgatcggtg tccggcccga
     6601 gtcgctggat gtcgcggagg agggaatccc ggccaaggtc gatgtcgtgg aggacgtcgg
     6661 agccgatgcg ttcgtgttct gcagcaccga actgggtgga gagacgatga agctcgtggc
     6721 acgctcggag gcgcggaagg cgccgaagca gggtgatcgt gtgaccctgc ggccgcgggc
     6781 cgacgaggcg catctcttcg atccggcgaa cggcgagcgg ctggaacgct gatgccccgc
     6841 gtgccgggcc cgcacaatcc gggcgagctg ttcctcggcg agatcagaga gcagcctcag
     6901 gcgctgctcc ggttgctcga tgaggaggcc gacttcgacg ccgtcagcaa ggtgatgaga
     6961 gagcgccagc cctcggtcgt ccggctggtc ggccacggct cgtccgacgc ggccgcatcg
     7021 ttcggcgtgt acgcattcgg gctactgccg ggatggactg cgatgcgcga ctcgatctcg
     7081 ctcaccgtct actacgacgc gaagatcgac ctctcgagct ctgtcgtgat cggcctctcg
     7141 cagtcgggcg agacgcccga tgtcgtcgag tacgtgaagc gtgctcgtgg acgcggggcc
     7201 ttcacggtcg ggatcacgaa cggagcggac tcgagccttg ccgcggcagc cgatgcaacg
     7261 ctggctctgt ctgctgggcc cgagcacgcg gtcgccgcat cgaggacgtt cttcaacaca
     7321 ctggcggcac tggcctgggt ggcaggagga gcggcgggaa gaggacgcga gatcgcagag
     7381 gggctgcgga ccgtctcggg tcagctttcg gaatcgctca cgttgctcga gcaggcggtc
     7441 tcgaacgtcg ccatggcttt cgcatatgtc gggcggatgt acgtgatcgg gcggggcatc
     7501 gagttcggca cggcgcgcga ggtcgcgctg aagctgaccg agacctgccg cgtcgctgcg
     7561 gagccactca ccgcgacgga tctcgcgcac ggcccgattg ctgcactcga ctcgatgttc
     7621 ccggtctgga cgatcgcgtc gcgggacgaa agcctcccgg ccgtcctcga ggcggccgcc
     7681 cgcgtcatcg aggccggagc gacggtcgtg gcgagcggta acgcagcggc ggagatcgaa
     7741 ggggctgcct acgtcctgcc ggtgccggag gcgccgcatc cgatcctctc gccgctcctt
     7801 tccgtggtcc cgggccagct ctttgcgggg gcgctggcgc acgccaaggg gctcgatgcc
     7861 gactatccgg tcggcctcag caaggtcacc cgggcacgtt gacttagacc gccagcgtct
     7921 gggctccatc gagttagctc tttcgggggg tagacgccac ccccggatcc gccgactgtg
     7981 ttcttggaga actgcggcaa ggatgactat cttcgagacc agccaagcgg cgtcggcttc
     8041 gccgactgtc ccacccttca gcaccgcgga gatgcggagc tatccgctcg gcacgctcgt
     8101 gtatcgctcg gggctgatgc cgctcgagac cgtcaacaga gctcttgcgg aagcggctga
     8161 aagcggacgg cggctcggag aggtactgct cgactacgga ttgcccgagc gcgatttgac
     8221 gcgtctgctc gcagcccaga acggacagga gtttgtcgac ctcaccgagt tcccgatcga
     8281 tccggaggtc gcgcgtttgc tgccgcagcc ggtcgctgag atgtactgcg ctttgccgct
     8341 gcagcgagag ggagactgca cgctggtcgc ggtgcccgat gccgacaacg cgtcgcatct
     8401 gaccaggctc caagcagcgc tgcacgggcc gattcggctt gtgaccgccg ctcgctctga
     8461 catcaaagac gccatcgaac gattgcacat cgagccgcaa gggatgatca cgcagatggc
     8521 gctcgccgag gcgaggacgc aggcgccgcc ggagccgacg acgacttatc gcatcgaagt
     8581 gacgctcgcg agcggtgcga ccctcctggt ggcagaagcg accgatttcg agtacgcgaa
     8641 gcaactgggc atgcgcgtga tcgacgaagc cgaggccggc ggcaccgtcg ccatcggcga
     8701 tgcgaccatc gacgggcggc aggtcgtgtc ggtcgacgtt ctcggtcgca acggcgcatg
     8761 aactttccgg tcaacgccgt tcgcgagctc atctccgcgg tgcgcaagga tggaaggccg
     8821 gttgtgcgcc tgacctgcgc cgattacggg acggagtggg tcgtcgctgc agacgtctat
     8881 ccggtcgacg agctcacggt gaaacccaag agcgccgggc cgtacgtgtt cgacaccgcc
     8941 caagaggctc gaagcttcat cgagagctcg ctcgtggcgc tgcagctgct cggctgcgaa
     9001 gtcgcgtagc tcctacggcc tcgatttctg gcgcgcgact gacgcgacct gcttccagcg
     9061 cttcttccgc gcgatgcgca gacgcgtgtc ggtgcgggca gcgatcgcct cgagctcccg
     9121 ctggagcttt cgccacgagc gcaagcggtc gagctccagc gagccgtcgt cgaccgcggc
     9181 caagaccgca cactccggct cgcgcatgtg cgcgcagttc cggaacttgc attccagcgc
     9241 aagcgcctcg atgtcctcga acgcggcgct gagatcgccc tcccagaacc ggagctcgcg
     9301 caggcccgga gtgtcgatga ccagcccgcc gccgggcagg cggacgagct cacgatgcgt
     9361 cgtggtgtga cgcccggttc cgtcggccgc gaccgcacgt gtgagcatca cttcctcatc
     9421 ggccagccgg ttgagcagag tcgacttgcc gacgcctgaa gacccgagta ggacgatcgt
     9481 gcggccgggt tgcaggtagg ggtcgagggc ttcgagccct tcgcccgtga cgttcgagac
     9541 ggggtggacc gggacgccga ttgcgaccgt ctcgactgcg agcacagcct cggggacatc
     9601 cggagaaagg tcggccttcg tgagcaccac gatcggttga gcgccgctct cccacgcgat
     9661 cgtgatgtag cgctccagcc ggcgcaggct gaagtcgtcg tcgagcccgg cgaggagaaa
     9721 ggccgcgtcg acgttggcgg cgagcacctg
//
LOCUS       DASVEU010000209         9614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_727668,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000209 DASVEU010000000
VERSION     DASVEU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727668"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VF225_08860"
     CDS             complement(<1..526)
                     /locus_tag="VF225_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145403.1"
                     /translation="MTETTVTPARPLVVEEGDVKPGFIGQSIPRKEDKRLVQGEGVFF
                     DDVKRHEMGFVHFVRSPYAHARIKSVDVSKALELDGVYGTLTGDEVAILTDPFFELST
                     PPGANIKDYALAVGRVRHVGDPVAAVVARTRELARDAAELVEVEYDPLPHVLDGEESL
                     RDEVVLHEDAGSNTV"
     gene            complement(523..993)
                     /locus_tag="VF225_08865"
     CDS             complement(523..993)
                     /locus_tag="VF225_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7145404.1"
                     /translation="MEAVARKTITVDANGEQYEREVDARRLLIHFLRDDLDLTGSHIG
                     CDTGNCGACSVLIDGTLVKSCMMLAVQADGTKIETVEGLASEGELNGLQQAFSEHHAL
                     QCGYCTPGMLMSATALLRQNESPSEDEIRKGIQGNICRCTGYVNIVEAIKAAAE"
     gene            complement(993..1799)
                     /locus_tag="VF225_08870"
     CDS             complement(993..1799)
                     /locus_tag="VF225_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7145405.1"
                     /translation="MLLREVEYARPASVQEALRLLGANDGARPLAGGQTLINVMKARA
                     ASPDVLVDLADIGELRGIELRADGTVELGAMTTYTEIIDSAEARARPILGEVCNQIAD
                     VQVRNRGTIGGNVCSNDPTNHLPPLLVAIGATMTIVGSDGERSVSAEEFFLGVYMTAA
                     GPGELLTKISVSPGKSDGFASVPIGKDGTCIVNVAASLDGGAVRLAIGCVDAVPVLLR
                     PESTEDEAVRSAVQGANLDPPWDVHASAGYRRHLAEVLALRAVAQAAGRN"
     gene            complement(1999..2071)
                     /locus_tag="VF225_08875"
     tRNA            complement(1999..2071)
                     /locus_tag="VF225_08875"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2036..2038),aa:Asn,seq:gtt)
     gene            complement(2113..3780)
                     /gene="dnaG"
                     /locus_tag="VF225_08880"
     CDS             complement(2113..3780)
                     /gene="dnaG"
                     /locus_tag="VF225_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7145406.1"
                     /translation="MARIKDTSVEEVKAAADMISVVSERTQLRKAGARYMGRCPFHEE
                     RTPSFSVNGVDKFYYCFGCGAKGDLITFVRETEQLDFAEAIEWLADRFNVQIEYEETS
                     PQQDARRRRRERLLELLEAAASFYERYLWDSQAGSLARDYLAGRGLGEEVCREYRLGL
                     ALGGTTLTRKALERGFTRDELVAAGLINRRGNDYFFGRLLFPLTDARGRVLGFQARKL
                     REDDPLKAKYVNSPEGELFRKGDLLYGLDRARAAIAKQERAIVVEGNTDVLALSQAGL
                     EPVVASMGTALTDRQLKELGRLTTRVWLCFDGDAAGEAATLRGMELAFAQGLDVRVVA
                     LPAGFDPADLAEGFEQLLTRAESYLAYRVRLEIERAADRQEAFVRVREVLSRFEESPE
                     RQDAIRYAADKLDLPKETQAGLAPRGAASATGRVSARLLDAGERLEQNALAGVAAHPA
                     LLPVLAELGPEHFDGEQHRRLREQLVSGGEPDADLVGLAAELDARASSEGIDERTAEE
                     LLLRLRARGIKRELLEADPAHTIELQASMAKILAAIEELASAQALSR"
     gene            complement(3780..4736)
                     /locus_tag="VF225_08885"
     CDS             complement(3780..4736)
                     /locus_tag="VF225_08885"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX7145407.1"
                     /translation="MPELAGRVADAFEAQVRELEERWLSPLAVRSYETRGRLRPEDDC
                     RLRTPFQRDRDRILHSKPFRRLKGKTQVFIDPAGDHYRTRMTHTLETTGIARGVARAL
                     RLNEDLVEAIGLGHDMGHTPFGHAGEDALDAALQERFGRTFRHNEQSVRIAESLNLTE
                     EVRDGILTHTGTQEPATLEGKIVRIVDRVAYINHDIDDAVRFGILDPAELPYEEVALL
                     GDRGSRRIDTLVHDLVESSERAGDIVQSEEIGGAMLALRSFMFERVYLGPHTRQEQER
                     ARATVRRIFEHLAERGEEPGQIVEFIAGMTDRFALSYVAELE"
     gene            complement(4747..5532)
                     /locus_tag="VF225_08890"
     CDS             complement(4747..5532)
                     /locus_tag="VF225_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7145408.1"
                     /translation="MDLGLKDRICLVTGSTAGIGLETARLLAAEGARVVVNGRNADRV
                     EQARADVGAALGVACDLSQPAAAERLVAETAAALGPVECLVNNVGVAYQVDFEDVTDE
                     QWDAMWQLNVMSYVRCIRAVLPAMKEAGAGTIVNVSSTAGKRPSTGMPNYSVTKAAVL
                     SLSRLVADLYAKHGVRCNAVAPGPTATAAWLGDGGLADQQAAASIKTRQQVLEAVGAG
                     RPLGRLAEPDEIAAVIVFLCSRRASYVTGSAWSADGGTVPIII"
     gene            5662..6243
                     /locus_tag="VF225_08895"
     CDS             5662..6243
                     /locus_tag="VF225_08895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7145409.1"
                     /translation="MTNLLLVRHGETDWNRDGRWQGGSDTRLNDVGREQARALAQQLD
                     GEIDVLYSSDLARARETAEIVAAKLGLEVRLDPRLRERGFGSWEGLTTTEIEERFADA
                     HRRWLAGEGAGADDAEAFEDFSARVEDFLSDVLRLHPGEEVLVVSHGGSIRVIHALAA
                     GVDYVRDHRLIPGVPNCSVARYGARDGKLAPLD"
     gene            6252..6491
                     /locus_tag="VF225_08900"
     CDS             6252..6491
                     /locus_tag="VF225_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145410.1"
                     /translation="MEDYTNKYKGEQVEVALFGGEYQEGVLTAYCYVEDVAHIELNGH
                     ILVPLQNIASLHCSSRCDAPEDDWPPRGLEQEDGN"
     gene            6502..7344
                     /locus_tag="VF225_08905"
     CDS             6502..7344
                     /locus_tag="VF225_08905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7145411.1"
                     /translation="MGGRLLVIRNVLKAWALLLGACALLGLIGWALGGYRLLSIFVFC
                     GVLLAGALYWYADRVALGLVRARELPVGGAPGLHSTIERLAARAGVAKPRLYLIDDGL
                     PRALATGRGPRGSTIAVSAGLLSAASPAELEAVLAHELAHVRHRDVVVQTSVVVLAAT
                     LLELSRVGGWFQRVLLWFLGPLASAFVHLLLSPKREFLADRAAAAISDTPHPMADALV
                     RLDQAAELVSFSGSPATEPLYTINPFLEVGLAALFVTHPPVGERVRRLRELDPDWREK
                     LRAA"
     gene            7341..8528
                     /locus_tag="VF225_08910"
     CDS             7341..8528
                     /locus_tag="VF225_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7145412.1"
                     /translation="MTLAIEQSEYEERLAALADHLLGRKLNGAVLFDPYYVLYYAGFA
                     FAPTERPIAFVLTADGKGGMLVPRLEREHAQANASVEHVADYPEYPSERHPMESLRGL
                     LEEMAVTGSFGADQDGYPWVLGYRGPSLTELTGTTPEKITAFVEDQMAVKSGAEIELI
                     RESSKWGNLAHVLLQRYTRPGLTESEVSRRASTEASLAMLDAIGPLYRAQSFFFDGAG
                     AGYRGQIGRNAAIPHALANNIVFQAGDVLVTGATAPVWGYLSELERTMVIGPPTPEQQ
                     RLFDHMVALQDVAFEAIKPGARCSDVDRAVRAYYDKHDLWDYWKHHVGHCIGLRYHEG
                     PFLDIGDDTQIKPGMVFTVEPGLYAAGLGGFRHSDTVAVTDDGIEFLTYYPRDLESLT
                     LPI"
     gene            complement(8525..9040)
                     /locus_tag="VF225_08915"
     CDS             complement(8525..9040)
                     /locus_tag="VF225_08915"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7145413.1"
                     /translation="MELRDGDILLRDWTLDDVPAIVAACNDAEILHWIPVIPRPYTED
                     DARAFVLREVPGVGTHQFAITANERVVGSIGMTVNDFRTGHIGYWCASENRGRGITTR
                     ALRILCKHSFEELELQRLELITDPDNRASQRVAEKVGFQREGVLRSHLLHPDRRRRDS
                     VMFSLLPGELV"
     gene            complement(9096..9212)
                     /gene="rrf"
                     /locus_tag="VF225_08920"
     rRNA            complement(9096..9212)
                     /gene="rrf"
                     /locus_tag="VF225_08920"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9276..>9614)
                     /locus_tag="VF225_08925"
     rRNA            complement(9276..>9614)
                     /locus_tag="VF225_08925"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1605 a         3250 c         3079 g         1680 t
ORIGIN      
        1 agacggtgtt cgacccggca tcctcgtgca gcacgacctc gtctctcagc gactcctcgc
       61 cgtcgagaac gtgcgggagc gggtcgtact cgacctcgac gagctcggca gcgtcgcgcg
      121 cgagctcgcg cgtacgtgcg accaccgccg cgacgggatc gccgacatga cgcacacggc
      181 cgaccgcgag cgcgtagtcc ttgatgttcg cccccggcgg cgtcgaaagc tcgaagaacg
      241 gatcggtgag gatcgcgacc tcgtcgcccg tcagcgtccc gtagacgccg tccagctcga
      301 gagccttcga gacgtcgacc gacttgatcc gtgcgtgcgc gtacggagag cggacgaagt
      361 ggacgaagcc catctcgtgc cgtttcacgt cgtcgaagaa gacgccctcg ccctggacga
      421 gtcgcttgtc ttctttgcgc gggatgctct ggccgatgaa gcccggcttg acgtcccctt
      481 cttcgacgac gagcgggcgc gccggagtga cggtagtctc ggtcactcag cggccgcctt
      541 gatcgcctcg acgatgttca cgtaacccgt gcagcggcag atgttccctt ggatgccttt
      601 gcggatctcg tcctcgctgg ggctctcatt ctggcgcagg agcgctgtgg cgctcatcag
      661 catccccggc gtgcagtagc cgcactggag cgcgtggtgc tctgaaaacg cttgctggag
      721 gccgttcagc tcgccctcgg aggcgaggcc ctcgaccgtc tcgatcttgg tcccgtccgc
      781 ctggacggcg agcatcatgc agcttttgac cagcgtgccg tcgatcagca cggagcaggc
      841 gccgcagttc cccgtgtcgc agccgatgtg gctcccggtc aggtcgaggt cgtcgcggag
      901 gaagtggatc aacaaccgtc gcgcatcgac ctcgcgctcg tactgctcgc cgttggcgtc
      961 gaccgtgatc gttttccgag cgactgcttc cactaattcc ttccggcggc ctgggcgacc
     1021 gcgcgtaggg cgagaacttc cgcgaggtgc cggcggtagc cggccgaggc gtggacgtcc
     1081 caaggcggat cgagattggc gccctgcacc gccgagcgga cggcctcgtc ttctgtcgac
     1141 tccggtctca ggaggacagg tactgcgtcg acgcagccga tcgcgaggcg caccgcgcca
     1201 ccgtcgagcg acgcggcgac gttgacgatg cacgtcccgt ccttgccgat cgggacggaa
     1261 gcgaagccgt cgctcttgcc gggcgatacc gagattttcg tcagcagctc tcccgggccg
     1321 gccgcagtca tgtagacgcc caggaagaat tcctcagcgg acacggagcg ctcgccgtcc
     1381 gagccgacga tcgtcatcgt cgcgccgatc gcgacgagca gcggagggag atggttcgtc
     1441 ggatcgttgg agcagacgtt gccgccgatc gtgccgcggt tgcggacctg aacgtccgcg
     1501 atctggttgc agacctcgcc gaggatcggg cgcgcacgtg cctccgcaga gtcgatgatc
     1561 tcggtgtacg tggtcatcgc accgagctcg accgtcccgt cggcgcggag ctcgatcccg
     1621 cgcaactcgc cgatgtcagc gaggtcgacg agaacgtcag gcgatgccgc gcgggccttc
     1681 atcacgttga tcagcgtctg tccgcccgcg agcgggcgag cgccgtcgtt tgcgccaaga
     1741 aggcgcaggg cttcctgaac gctcgcggga cgagcgtact cgacctccct caggagcatg
     1801 cgctattcag ttcagaaacc cctgctcagg tcaagaatca accgaagccc ttgcctcgcc
     1861 gcaatcgtca cagacacacg acggaaagcg ggccgcgcgg acgcgcgacg aagcgatagg
     1921 cgagggtccc cctcgccgcc gactgaatct cggaacccgc cggaaaacat gcgcgaagcg
     1981 cgttttccgg cggccgagct ccccaggttg gactcgaacc aacaaccctc cggttaacag
     2041 ccggatgctc tgccaattga gctactgggg aaagcagggg gctagtttag ccgttgaagt
     2101 ctggactcga cctcagcgcg agagcgcctg tgcagaggcc aattcctcga tcgcggcgag
     2161 gatcttggcc atcgaggctt gcaactcgat ggtatgcgcc ggatctgcct ccagtagctc
     2221 gcgtttgatc cctctcgcac ggaggcgaag caaaagctcc tcagccgtcc gctcgtcgat
     2281 cccttccgag ctcgcacgcg cgtcgagctc cgcggcgagg cccacgaggt cggcatcagg
     2341 ctcgccaccc gacaccagct gctctcgtag ccgtcggtgc tgctcgccgt cgaagtgctc
     2401 gggcccgagc tcggcgagca cgggcagaag ggcaggatgg gcggcgactc cggcgagagc
     2461 attctgctcg agccgctcgc cggcatcgag caatctcgcg gaaacacgtc ccgtggccga
     2521 cgcagcacct cgcggagcaa gtccggcctg tgtctccttc ggcagatcga gcttgtccgc
     2581 ggcatagcgg atggcgtcct gccgttcggg tgactcctcg aaacgcgaga gcacctcccg
     2641 gacgcggacg aacgcttcct ggcggtcggc cgcccgttcg atctcgaggc gaacgcggta
     2701 ggccagatag ctctccgccc gcgtaagcaa ctgctcgaat ccctccgcca gatcggccgg
     2761 atcgaaacca gcgggcagcg cgacgactcg aacgtcgaga ccctgtgcga acgccagctc
     2821 catccctcgt aaggttgccg cctcacctgc ggcatcaccg tcgaagcaga gccacacgcg
     2881 cgtcgtcagc cgtccgagct ccttcagctg ccggtcggtc agcgcggttc ccatcgacgc
     2941 gaccaccggc tcgagcccgg cctggctgag cgcgagcacg tcggtgttcc cctcgacgac
     3001 gatggcgcgt tcctgcttcg cgatcgcggc ccgggcacgg tcgagcccgt agagcaagtc
     3061 gcctttccgg aacagctccc cttcaggtga gttgacgtac ttcgccttca gcggatcgtc
     3121 ctcgcgcagc ttgcgcgcct gaaagccgag cacgcgaccc cgtgcgtccg tgagtggaaa
     3181 gagcaaccgc ccgaagaagt agtcgttgcc gcgcctgttg atgaggcccg cggcaaccag
     3241 ttcgtcgcgc gtgaacccgc gctcgagcgc cttccgcgtc aatgtcgttc cgccgagcgc
     3301 caacccgagc cggtactcac gacacacttc ctcgccgaga ccgcggccgg cgaggtagtc
     3361 gcgcgccagc gagccggcct gcgagtccca gaggtatcgc tcgtagaacg acgcggctgc
     3421 ttccagcaac tcgagcagac gctcgcgccg acgccgccga gcatcttgct gaggcgacgt
     3481 ttcctcgtac tcgatctgga cgttgaagcg gtctgcaagc cactcgatcg cttcggcgaa
     3541 atcgagctgc tcggtttcgc gaacgaaagt gatcaggtcg cctttcgccc cgcagccgaa
     3601 gcagtagtag aacttgtcca ccccgttcac ggaaaagctg ggcgtccgct cttcgtggaa
     3661 cgggcagcgc cccatgtaac gggcgccggc cttgcggagc tgagtgcgct ccgacacgac
     3721 cgagatcatg tccgcggcag ctttgacctc ttcgaccgac gtgtctttga tccgggccac
     3781 tactccagct ccgcgacgta gctcagcgcg aatcggtcgg tcatccctgc aatgaactcg
     3841 acgatctgcc cgggctcctc gccacgctcg gcgagatgct cgaagatccg ccgcacggtt
     3901 gcccgcgcgc gctcctgttc ctgccgcgtg tgcgggccta ggtagacccg ctcgaacatg
     3961 aacgaacgca gtgcgagcat tgccccgccg atctcctcgc tctgcacgat gtctccggca
     4021 cgctcggagc tctcgaccag gtcgtgcacc agtgtgtcga tgcgtcgcga gccccggtcg
     4081 ccgaggagcg caacctcctc gtagggcaac tccgctggat cgaggatccc gaagcggacg
     4141 gcatcgtcga tgtcgtggtt gatgtacgcg acacggtcga cgattcgaac gatcttgccc
     4201 tcgagcgtcg ccggctcctg cgtcccggtg tgagtgagga tcccatcgcg aacctcctcc
     4261 gtcaggttca gcgactccgc gatccgcacc gactgctcgt tgtgacggaa ggtccggccg
     4321 aaacgctctt gcagcgcggc gtcgagcgca tcctcgccgg cgtggccaaa gggcgtgtgc
     4381 cccatgtcat ggccgagccc gatcgcttcg acgagatcct cgttcaaccg cagggctcgc
     4441 gccacgccac gggcgatacc cgtcgtctcg agcgtgtgag tcatgcgcgt gcggtagtga
     4501 tcgcctgcgg gatcgatgaa gacctgagtc ttccccttca gtcgccgaaa cggcttcgaa
     4561 tgcaagatgc ggtcgcggtc gcgctggaac ggcgtgcgca ggcggcagtc gtcctccggg
     4621 cggagtcgtc cccgcgtctc gtaggagcgg accgccaacg gcgagagcca ccgttcctcc
     4681 agctcgcgca cctgcgcctc gaaggcgtcc gcgactcttc ctgcgagctc tggcatgagt
     4741 ctcaggctag atgatgatcg ggacggttcc gccgtccgcg ctccaggctg aaccggttac
     4801 gtaggaagcg cgccgggagc agagaaatac gatgacggcg gcgatttcat cgggctcggc
     4861 gagtcggccc agcgggcgcc cggctccgac ggcctcgagc acttgttgac gcgtcttaat
     4921 agaagcggcc gcctgctggt ctgccaaacc tccgtcaccc agccacgcgg cggtcgccgt
     4981 cgggcctggt gcgacagcat tacagcgcac gccgtgcttg gcgtagagat cggccacgag
     5041 acgcgagagt gagagtaccg ccgccttcgt gaccgagtag ttcggcatgc ctgtcgacgg
     5101 acgcttccca gccgtcgacg agacgttcac gatcgtgccc gcacctgcct ccttcatcgc
     5161 cggcagaacg gcgcggatgc agcgaacgta gctcatcaca ttcagttgcc acatcgcgtc
     5221 ccattgctcg tccgtcacgt cctcgaagtc gacctggtag gccacgccga cgttgttcac
     5281 caggcactcg acggggccga gggccgccgc tgtttcagcg acgagccgtt ccgccgcggc
     5341 gggctgcgac aggtcgcatg cgactccaag tgcggcgccg acatcggcgc gggcttgctc
     5401 gacccgatcg gcgtttcgcc cgttgaccac cacacgcgcg ccttccgctg cgagcagccg
     5461 cgcggtctcg agcccgatgc ccgcggtcga gccggtgacg aggcagatcc gatctttgag
     5521 gccgagatcc atgggctggg tagcctagag cctctggtat ggattccaac gtctcgaccg
     5581 gtcagttcag acggacgcgc gggcagagcc cgtacgtccg gacgagcgca ccgcaagcgg
     5641 cgccctagtg ccgtgactgg catgaccaac ctgctcctgg ttcgccacgg cgagacggat
     5701 tggaaccgcg acggccgctg gcaaggcggc tccgacacgc ggctgaacga cgtaggccgc
     5761 gagcaggcgc gggcgctcgc tcaacagctg gatggcgaga tcgacgttct ctattcgagc
     5821 gacctcgccc gggcgcggga gacggcggag atcgtcgcgg cgaagctcgg cctcgaggtt
     5881 cgactcgatc caaggctgcg ggagcgcggg ttcggatcgt gggaggggct cacgacgacc
     5941 gagatcgagg aacggttcgc cgacgcccac agacgctggc tcgccggcga gggcgccggg
     6001 gcagacgacg ccgaggcctt cgaggatttc tctgcgcgcg tagaggactt tctttcagac
     6061 gtgctgcgcc tccatcccgg cgaggaggtc ctggtggttt ctcacggcgg ctcgatccga
     6121 gtcatccatg ccctggcggc cggcgtcgac tacgtccggg atcacaggct gattccaggc
     6181 gtgcccaatt gctcggtggc cagatacggg gctagggacg ggaaactggc gccgctagac
     6241 tgaggccccc tgtggaggac tacaccaaca aatacaaggg cgagcaggtg gaggtcgcgc
     6301 tgttcggggg cgagtatcag gaaggtgtcc tgacggccta ttgctatgtg gaggacgtcg
     6361 cccacatcga gctgaacgga cacattctcg ttccgctgca gaacatcgcc tcgctgcact
     6421 gttcgagccg ttgcgacgcc ccggaggatg actggcctcc gcgcgggctc gagcaggaag
     6481 acggcaacta gggcccccgt tttgggaggc cggcttctcg tcattcggaa cgtcctcaag
     6541 gcctgggcct tgctgctcgg agcctgcgca ctgctcgggc tgatcggctg ggcactcggc
     6601 ggctatcgcc tcctctcgat cttcgtcttc tgcggcgtgc tgctcgcggg cgctctctac
     6661 tggtacgcag accgcgtcgc actcggactg gtacgtgcac gcgagctccc ggtcggaggg
     6721 gcgcccgggc tccactccac gatcgagcga ctcgcagctc gcgcgggcgt cgcgaagccg
     6781 cggttgtact tgatcgacga cgggctgcca cgggcgctcg ccaccggccg agggccccgt
     6841 ggctcgacga tcgcagtgag cgcgggcctc ctatctgcgg cgtctccggc ggagctcgag
     6901 gctgtgctgg cacacgagct cgcacacgtg cgccatcgcg acgtcgtcgt gcagacgtcc
     6961 gtcgtcgtgc tcgccgcgac gctgctcgag ctgagcagag tcggcggttg gttccagcgc
     7021 gtcctgctgt ggttcctggg gccgctggcc tcggcgttcg tccatcttct gctctcaccg
     7081 aagcgcgagt tcctcgcaga ccgcgctgct gcagcgatct ccgacacgcc gcacccgatg
     7141 gccgatgccc tggtgcgcct cgaccaggcc gccgagctcg tttcattcag tggcagcccg
     7201 gccaccgaac cgctctacac gatcaacccg tttctcgagg tcggcctggc tgcgctcttc
     7261 gtgacgcatc cgcccgtagg cgaacgcgtg cggcggctgc gcgagctcga tcccgactgg
     7321 cgtgagaagc tccgcgcggc ttgaccctcg cgattgaaca atccgaatac gaggagcggc
     7381 tggcagcgct cgccgaccac ctgctcgggc gcaagctgaa tggcgccgtc ctcttcgacc
     7441 cctactacgt cctctactac gccggctttg ccttcgcgcc cacggagcgc ccaatcgcct
     7501 tcgtcctgac cgctgacggt aagggcggca tgctcgttcc tcggttagag cgcgagcacg
     7561 cacaagcgaa tgccagcgtc gagcacgtcg cggactaccc ggagtacccg tcggagaggc
     7621 atcccatgga atccctccgc ggcctgctcg aagaaatggc tgtcacgggg tccttcggcg
     7681 ccgaccagga cgggtatccc tgggtactcg gctatcgagg gccttcgctc accgagctga
     7741 ctggaacgac tcccgagaag atcaccgcct tcgtcgagga ccagatggcg gtcaagagcg
     7801 gcgcggagat cgaactgatt cgcgagagct cgaaatgggg caacctcgcg cacgttttgc
     7861 tgcagcggta cacccggccc gggttgacgg aaagcgaggt gtcccggcgc gcaagcacgg
     7921 aggcctcgct cgcgatgctc gacgcgatcg gcccgctgta tcgcgcgcaa agcttcttct
     7981 tcgatggggc cggcgcaggc tatcgcgggc agatcgggcg caacgcggca atcccgcacg
     8041 cgctcgccaa caacatcgtg ttccaggccg gcgacgtcct cgtgaccgga gcgaccgcgc
     8101 cggtgtgggg ttacctgtcg gagctcgagc gcacgatggt tatcgggccg ccgacacctg
     8161 aacagcagcg tctgttcgac catatggtcg cgctacagga cgtcgcgttc gaggcgatca
     8221 agcccggcgc acgctgctct gacgtcgacc gcgcagtccg cgcgtattac gacaagcatg
     8281 atctctggga ctactggaag caccacgtcg ggcactgcat cgggctccgc taccacgagg
     8341 gacccttcct cgacatcggc gacgacacgc agatcaagcc cgggatggtg ttcaccgtcg
     8401 agcccgggct ctatgcagcc ggtttggggg gcttccggca ctcggacacc gtggccgtga
     8461 cagacgacgg aatcgagttc ctgacctact acccgcgcga cctcgagagc ctgacgctcc
     8521 cgatctagac gagctcgcct ggaagcagcg agaacatcac cgagtcgcgg cgccgccggt
     8581 ccggatggag gaggtgcgac cgcagcactc cttcccgttg gaatcccacc ttctccgcaa
     8641 ctctttgcga agctcggttg tccgggtcgg tgatcaactc cagccgctgc agttcgagct
     8701 cctcaaacga gtgcttgcag agaatccgca gcgctcgagt tgtgatacca cggccgcgat
     8761 tctctgaggc gcaccagtag ccgatgtgac cggtacggaa atcgttgacc gtcattccga
     8821 tcgagcctac gacacgttca ttggcagtga tggcgaactg gtgcgtcccg acacccggga
     8881 cctctctcag gacgaatgcg cgcgcgtcgt cctccgtata cgggcgcggg atgacgggaa
     8941 tccaatgcag gatctccgcg tcgttgcacg ccgcgacgat cgcaggaacg tcgtccagcg
     9001 tccagtcacg cagaaggatg tcgccgtcgc gcagctccac ttttccaagc ctagaacgac
     9061 gaaaggccgc gttagcggcc cttcgtcgaa aagataatcg gcggctgcct actctcccgg
     9121 gaggttgccc tccgagtacc atcggcgcaa gcaggcttaa cttctctgtt cggaatggga
     9181 agaggtgttt ccctgccgct atgaccgccg aagttgtcag ggcgctgcgc gtttccgcgc
     9241 accctcaaaa ctccatagct ccacactcgg tgttcaaaat caagacctcg agcaattagt
     9301 acgggtccgc tgaacgtgtc gccacgcttg cacgtcccgc ctatcaacgt ggtggtctcc
     9361 cacggctctt actccctcta ggggatggga agcctcatct cgaggtgggc ttcccgctta
     9421 gatgctttca gcggttatcc catccagacg tggctaatca gcagcgccgt tggcacgacg
     9481 actgatacac cagaggtctg ttcgctccgg tcctctcgta ctaggagcga ctcctctcaa
     9541 gcttccagcg cccatggtgg atagggaccg aactgtctca cgacgttctg aacccagctc
     9601 gcgtaccgct ttaa
//
LOCUS       DASVEU010000210         8972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_921204,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000210 DASVEU010000000
VERSION     DASVEU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921204"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF225_08930"
     CDS             complement(<1..344)
                     /locus_tag="VF225_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145414.1"
                     /translation="MSSVFGNSAIERWTRLILRLRWLVFGIWLAVLIAGGFASTRLSG
                     LLSNDFGVPGTDSAQVQSILQRRFGDRSDGEYLVVFKVAGAVDRPLLARLQQTVDRAV
                     SIVPTARAGPLRA"
     gene            complement(445..1587)
                     /locus_tag="VF225_08935"
     CDS             complement(445..1587)
                     /locus_tag="VF225_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX7145415.1"
                     /translation="MRVRRSLVAIAALVAAVSASIALAGNAKPASADGGGIATRSYAQ
                     TNLVSDIPGLAAHTDPNLKNPWGTSVGPGSPIWVSDNHAGVTTLYDGAGNPQPLVVAI
                     PAPPSAGEGAVGAPTGQAFNTFDPTSSDFVISENGNSGPAFFLFATEDGTIAGWNPNV
                     DRPHAVIAVDRSTATDSAGDVGALYKGLALVNTPAGKFLYASNFRFGTVDVFDSHFKL
                     VNSFTDPTIPAGFAPFGIHNIGGKLFVTFAKQGPGKEDDAARPGNGFVDVFAPNGDLL
                     QRLVSRGELDSPWAVTLASPTFGPFGGDILVGNFGNGRINAYDPVSGEFQGKLSGPGG
                     GPVVIDGLWGLRFAPATPGAGPNTLFFTAGLNDEADGLFGTIVPNG"
     gene            complement(1635..2984)
                     /locus_tag="VF225_08940"
     CDS             complement(1635..2984)
                     /locus_tag="VF225_08940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX7145416.1"
                     /translation="MKRFTVRLFALGLTLGALGAAAVLAGGAYAGTTQTNAMKARGPA
                     RHARSAKESGQPVIDWNQVLLSIVNTPGAQPANIQPTRNFAILHAAIYDAVNAIDRTH
                     EPYLISVRAPRDASETAAADAAAHTALVGLYPTLQNSLDSDYAAELAQVPNGPAKDQG
                     VSLGEQVASDLLAIRANDGSNVIPPPFVAGTNPGDYAPTPPNFPTPVFTTWGQVRPFV
                     LERGDQFRPTPPPALTSDAYAAAINEVQSLGSVTSTTRTAEQTEIGKFWNPPIQNFWN
                     QIAQTVALARHSDLPTTARLFAALNLSFADSAIAFYDAKYTFRLWRPVTAIRLADTDG
                     NPLTVADPTWLPLSGNTAADPSYPGAHSTISAAGADVLASFYGRRQSFSVTSSALPGV
                     TRSFTSFGAAAEEAGLSRIFSGQHTRLDHLAGLTLGHDVAGFVLHNALLPAHDSSGK"
     gene            complement(3043..3252)
                     /locus_tag="VF225_08945"
     CDS             complement(3043..3252)
                     /locus_tag="VF225_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145417.1"
                     /translation="MSIYELDIGWATTASERRYLHWMLVACDEVGGVFLTARDDVLAV
                     LFNGDRRGFDALARTLAPETLGTEP"
     gene            complement(3249..4151)
                     /locus_tag="VF225_08950"
     CDS             complement(3249..4151)
                     /locus_tag="VF225_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7145418.1"
                     /translation="MSERPTIDEQSATSAATIWPISFAFGVAVLLVGLIVNPEVIAPL
                     GAAITVVAAAAWIRGGRRGQQPTSSEQTAPQEVERGGERYTRNRFLERATLGLGGVIA
                     GAVALPAAGLAVLPSFLGQRRRAVDLGPVSAFPEGRFVVATFLADPKTGEVSRRAAYI
                     RNNGLLAGLPSFTVMSSRCTHVGCPTQPNGPTLNQQRHAEQTANGEVGLVPTLPAGGF
                     GCPCHGSEFDTEGNRTAGPAPRALDRYEFSIRHGRLWLNRLFSVSHVEGQGASARIHR
                     FSLRGAGEPADGPESWLYPIDPIR"
     gene            4450..5592
                     /locus_tag="VF225_08955"
     CDS             4450..5592
                     /locus_tag="VF225_08955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7145419.1"
                     /translation="MPRAELASVWSTREAVGRKHDRSGDTHIELEFRSQALQLGFWLG
                     WLSIIALFGGLADAGSKHRWLLLLLTLTAATCNAAAMFVPWREWLQIRRGRLLLDLWS
                     GGLIGFIALLVFTGGPNFALLLFLAIPFIAVVQVGWRRGFWLAVSAGTCALAAAFVPL
                     PVGATAMRLALIAAAVTVVLVLARRIRREAAAHKDAAFRAELERTLTTEANHRIKNDL
                     QTAADLLLLGRPRGGDGRAFDDTAARIQSIATVHRLLTETHDPVDASALLNSIADSAP
                     VPVAIEAEPAALDASTAQKLGIVANELITNAFQHGAPPIAVRLDSGEKHRLRVDDCGD
                     GADGAPGLGLQLVRRVVEHGLRGSFALTTRPGGGTRAEAVFPAEPR"
     gene            5589..6194
                     /locus_tag="VF225_08960"
     CDS             5589..6194
                     /locus_tag="VF225_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145420.1"
                     /translation="MKILIAEDDPVVALGLTERVRSLGHDPIGPVGDGEEAVAEAKAS
                     LPDLYLFDIEMPNLDGLAAAVQLAGEGLRRPVVIVTGVDDPSLVERSIASGVSAYLTK
                     PIDTRELEAAIKLAAARHAEFEALEAEVSKAQQALEDRKLVERAKGLLMSALKLSEQD
                     AFRRLQMTARERNLRLADVARRIVEQQSLLDPKQDSPAASP"
     gene            complement(6330..6902)
                     /locus_tag="VF225_08965"
     CDS             complement(6330..6902)
                     /locus_tag="VF225_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7145421.1"
                     /translation="MSSIPAIAQRTLGRVLPPWWLFLITGIAWTVVALIVLRFDYTSV
                     NAIAILFGVIAIAAGGFEIGMTMLAQGWWKLLYGVLAGVFIATGIISFIHPGDTFLAL
                     AAVISFFLVFAGTFDIIMAISARREIDVWWLQLIGGIIELALGFWAAGYYGRSAVLLV
                     AWVAAFAVIRGVTNIVLAFRVRELQHAAPV"
     gene            complement(7054..7740)
                     /locus_tag="VF225_08970"
     CDS             complement(7054..7740)
                     /locus_tag="VF225_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145422.1"
                     /translation="MRRAAIAALLTAWFLVVTAGAEAKEPPAGFQLCGSNGCATIGMD
                     DGEPLAISLFFGGGSVELWTPAATPAPFYSLRWSYQQGQLHTAYFVPDLNMVRFVGDS
                     ASATVDPRGAIHWLRLDSRARAILDRIAATLQPTPVPSPSTVMVGGKAVQDPASYLRL
                     WSVGKPTYSWPRGGFLKIKVTCDIASPWTDESAHLSIARRRPFLLRDSTILRIPAKLA
                     QQVRARVSLR"
     gene            complement(7860..8273)
                     /locus_tag="VF225_08975"
     CDS             complement(7860..8273)
                     /locus_tag="VF225_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145423.1"
                     /translation="MPGDQVSSGPEDEHRVFRVGGISYLRIPAEDSRRSAAFYEAVFG
                     WSLRGDPDDPSFDDGTGHVIGHFMTDLPVAGEAGVCPYIFVERVDETLEKVVAHGGAV
                     VTAPYPEGDLWVATFRDPPGNVIGVWQLGSADVTE"
     gene            complement(8371..>8972)
                     /locus_tag="VF225_08980"
     CDS             complement(8371..>8972)
                     /locus_tag="VF225_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145424.1"
                     /translation="LFLMLTLYMQQVLGYSPMKTGVAYLAVAGSAILWSAVAAQLVNR
                     IGVKPVLVTGMVALTTGLVYFTQVSVHGSYFSDLLPGFLLVGVGLGFSFVPISIAALA
                     GVRPAEAGLASGLFNTTQQIGGALGIAVLSTIATSRTSHALADGSALPSALVHGFTGA
                     FVVGAGIAAVGIVAALTLIRRDELEQAPEELEPVLDLAA"
BASE COUNT         1581 a         2961 c         3019 g         1411 t
ORIGIN      
        1 gctcgcagcg ggccggcacg agcggtcggg acaatcgaca cggcgcggtc gacggtttgc
       61 tgcaggcgcg ccagcagcgg tctgtcaacc gcgcccgcga ccttgaagac gaccaggtat
      121 tcgccgtcgc tgcgatcgcc gaagcgccgc tgcaggatcg actgcacctg agcagagtcg
      181 gttcctggga cgccgaaatc gttcgagagc aatccgctga gtcgcgtcga cgcaaagccg
      241 cccgcaatca gcacggccag ccagatgcca aacacgagcc agcgcagccg caggatcaaa
      301 cgcgtccaac gctcgatcgc ggagtttccg aaaacagatg acacagtcga tcgagccgct
      361 ctcctcgtcg aagttccgac cgcccggctc cgagtgaggc gcacgcggca cctcactcgt
      421 agcctggacg ttctaccgtg ggcgctaccc gttggggacg atcgtcccga acagcccgtc
      481 ggcttcgtcg ttgaggccgg ctgtgaagaa caacgtgttc gggccggcac ccggcgttgc
      541 gggcgcaaag cggagtcccc acagcccgtc gatcacgacc gggccgccgc caggcccgct
      601 gagcttgccc tggaactcgc cgctcaccgg gtcgtacgcg ttgattcggc cgttgccgaa
      661 gttgccaacc aggatgtcgc caccgaaggg accgaaggtc ggcgacgcca gcgtcactgc
      721 ccagggagag tcgagctcgc ctcggctgac tagccgctgg agcaggtctc cattcggggc
      781 gaagacgtcg acgaatccat tgccgggccg ggcggcgtca tcctctttgc ccggaccttg
      841 cttggcgaag gtcacgaaga gcttgccgcc gatgttgtgg atcccgaagg gggcaaaccc
      901 ggccgggatc gtcggatccg tgaagctgtt cacgagcttg aagtgactgt cgaacacgtc
      961 gacggtcccg aaacggaagt tgctcgcgta gaggaacttg ccggccggcg tgttcaccaa
     1021 ggccaaaccc ttgtagagcg cgccgacgtc acctgcgctg tcggtcgcgg tcgatcggtc
     1081 gacggcgatg accgcgtgag gccggtcgac gttcgggttc cagcccgcga tggttccgtc
     1141 ttcggtcgcg aacaggaaga aggcgggacc ggagttgccg ttctccgaga tcacgaaatc
     1201 ggacgaggtc ggatcgaagg tgttgaacgc ctggccggta ggcgcaccga cggcgccctc
     1261 ccctgcagac ggtggtgccg ggatggcaac cacgagcggt tgagggttgc cggcgccgtc
     1321 gtacagcgtc gtgactccgg catggttgtc ggagacccag atcgggctgc ccgggccgac
     1381 cgacgtgccc cagggattct tcaggttcgg gtccgtgtgc gcagccaaac ccgggatgtc
     1441 cgaaacgagg tttgtctgcg cataggaccg tgtggcgatc ccgccaccgt cggctgaggc
     1501 aggcttagcg ttccccgcca gggcgatcga cgctgacacc gccgcgacga gcgcggcaat
     1561 cgcaacaaga gatcgtcgta ctctcatgat tctcccttcg tgtttgggta gtgcctttcg
     1621 ttgtgaccga gccgctactt gcccgaggag tcgtgagcgg gaagtagcgc gttgtgaagg
     1681 acgaagcccg cgacgtcgtg gccgagcgtg aggccggcga ggtgatcgag ccgcgtgtgc
     1741 tggccggaga agattcggct cagaccggcc tcttccgcgg cagcgccgaa gctcgtgaac
     1801 gagcgcgtca cgccgggcag cgccgacgag gtgaccgaga acgactgccg gcgaccgtag
     1861 aagctggcga gcacgtcggc acccgcggcg ctgatcgtgc tgtgcgcacc ggggtacgac
     1921 gggtcggccg ccgtgttccc ggagagcggg agccacgtcg ggtcggcaac cgtcagggga
     1981 ttgccgtcgg tgtcggcgag gcggatcgca gtcaccggcc gccagagccg gaacgtgtac
     2041 ttggcgtcgt agaaggcgat cgccgagtcg gcgaagctca ggttgagggc cgcgaacagt
     2101 cgcgccgtcg tcggcaggtc gctgtggcgc gcgagtgcaa cagtctgggc gatctggttc
     2161 cagaagttct ggatcggtgg gttccagaac ttgccgatct cggtctgctc ggccgtgcgg
     2221 gtcgtgctgg tcaccgagcc gaggctctgc acctcgttga tcgccgccgc gtaggcgtcg
     2281 ctcgtgagcg cgggaggcgg ggtcggacgg aactgatcgc cgcgctcgag tacgaacggc
     2341 cttacctgcc cccaggtggt gaacactggt gtcggaaagt tcggcggagt cggggcgtaa
     2401 tcgccgggat tcgtgcccgc gacgaacggg ggtggaatga cgttcgaccc gtcgttcgcc
     2461 cgaatcgcaa gcagatcgct cgcaacctgc tcgccgagac tgacgccctg atctttcgcc
     2521 ggtccgttcg gcacctgggc gagctcagcc gcgtagtcgg agtcgaggct gttctgcagc
     2581 gtcgggtaga gcccgaccag ggcggtgtgt gcggccgcgt cggcggcagc ggtctccgag
     2641 gcgtcgcgag gcgcgcgcac ggagatcagg tacggctcgt gggtgcggtc gatcgcattg
     2701 accgcgtcgt agatcgcagc gtggaggatg gcgaagttgc gtgtcggttg gatgttcgcc
     2761 ggctgcgcac ccggcgtgtt cacgatcgag agcagaacct ggttccagtc gatcaccggt
     2821 tgcccggact ccttcgcact ccgggcgtgc ctcgcgggac cgcgggcctt catcgcattc
     2881 gtttgggtcg tgccggcgta ggcgccgccc gcgaggactg cagctgcgcc aagcgcgccg
     2941 agtgtgagtc cgagcgcgaa cagccgtacg gtgaatcgtt tcatgacagt gctccttctt
     3001 cgttggtggt tgcagtcgtg tgttgtggga gcaagcggcg aatcacggct ccgtccccag
     3061 cgtttccggc gcgagtgtcc gagccagggc gtcgaagccc ctgcgatctc cgttgaagag
     3121 aacggcgagc acgtcgtccc gcgcggtcag aaagacgccg ccaacctcgt cgcaggcgac
     3181 cagcatccag tgcaggtaac gccgctcgct cgccgtcgtc gcccagccga tgtcgagttc
     3241 gtagatgctc atcggatcgg atcgatgggg tacagccacg actcgggtcc atccgccggc
     3301 tcgccggcgc cgcgcaggga gaagcggtgg atcctcgcgc tcgctccctg cccctcgacg
     3361 tggctgacgc tgaacaagcg gttgagccag aggcggccgt ggcgaatcga gaactcgtag
     3421 cgatcgagcg cgcgcggggc aggacccgcc gtgcgattgc cctcggtgtc gaattcgctg
     3481 ccatgacatg ggcagccgaa gccgccggcg ggcaacgtcg ggactagccc gacctcgccg
     3541 ttcgccgtct gttcggcatg gcgctgctgg ttgagcgtcg gcccgttcgg ttgggtcgga
     3601 caaccaacgt gcgtgcagcg gctcgacatc accgtgaagc tcggcaatcc agcgagcaag
     3661 ccgttgttgc ggatgtaggc ggcacgtcgc gatacctcgc cggtcttcgg gtcggcgagg
     3721 aacgtcgcca cgacgaaccg gccctctgga aaggcactca ccggtccaag gtcgaccgcg
     3781 cgccgccgct gaccgaggaa ggagggcaag acggccaacc cggcggcggg taacgccacg
     3841 gcgccggcga tgacgccgcc gaggccgagc gtcgcgcgct cgaggaagcg attgcgtgtg
     3901 tagcgctcgc cgccccgctc aacttcctgc ggggcggtct gctcgctcga ggtcggctgc
     3961 tgtccccgac gaccgccgcg tatccaggcg gcagcagcca cgacggtgat tgcggcgccc
     4021 aacggcgcga tcacctccgg gttcacgatc agcccgacga gcagcacagc gacgccgaac
     4081 gcgaaactga tcggccaaat cgtcgccgca ctcgtcgcgg attgctcatc aatagtcgga
     4141 cgctcactca tcgcctctcc ctgtcgaatc acgcgagccg gacggctacg cggggaaaga
     4201 ggcgatcaag gccccgagcg acgtcgtcat gaacgccgcg aaaatcacgc tagcaggagc
     4261 ggtcgaccct gagtggcaac taacgtctgc acctcgatca ggtcggcgcg cttacaacaa
     4321 aaccgaagac gtcagctctg cgtaaccgcg agaagaggac gagttgtctt taccgcgcac
     4381 tcaaacaagg aaccggacta aaaccgtgct cgccgtcgtt gttggcttcg tgggtggatc
     4441 ttgctaggag tgccgcgcgc agaattggcg agtgtctggt caacgcgtga ggcggtaggc
     4501 cgtaagcacg acagaagtgg cgatacgcac atcgagctcg agttccggtc gcaggctctc
     4561 cagctcggat tctggctcgg ctggctctcg atcatcgccc ttttcggcgg cctcgccgac
     4621 gccggatcga aacaccgctg gttgctgctg ctcctgacgc tcacggcggc cacctgcaac
     4681 gcggccgcca tgttcgtccc ctggcgcgag tggctgcaaa tccgccgggg ccgactattg
     4741 ctcgacctct ggtccggagg attgatcgga ttcatcgcgc tgctcgtctt caccggcggc
     4801 ccgaacttcg cgctcctcct cttcctcgcg atcccgttca tcgcggtcgt ccaggtcggc
     4861 tggcgccggg gtttctggct cgccgttagc gccggaacgt gcgcgcttgc ggccgcattc
     4921 gtcccactcc cggttggggc aaccgcaatg cggctcgcgc tgatcgccgc ggcggtcaca
     4981 gtcgtgctcg tcctcgcccg aaggatccgt cgcgaagcgg ccgcgcacaa ggacgccgcc
     5041 ttccgcgccg agctcgagcg cacgttgacg acggaggcca atcaccggat caagaacgac
     5101 ctccagaccg ccgccgacct cctgctactc ggccgcccgc ggggcggcga cggtcgtgcc
     5161 ttcgacgaca ccgccgcccg catccaatcg atcgcgactg tccatcggct gctgacggag
     5221 acccacgacc ccgtcgacgc aagcgcgctc ctgaacagca tcgcggactc ggcaccggtg
     5281 ccggtcgcga tcgaggccga gcccgcagcc ctcgacgctt ctacggccca gaagctcgga
     5341 atcgtcgcca acgagctgat cacgaatgcc tttcagcacg gcgctccccc aatcgccgtg
     5401 cgcctggatt cgggagaaaa gcatcgccta cgcgtcgatg attgtggtga cggtgccgac
     5461 ggtgcgccag gtctgggtct tcagctcgta cgacgggtgg tcgaacacgg acttcgcggg
     5521 agcttcgcgc tgaccacacg acccggcggc ggcactcgcg ccgaggccgt ctttcccgcg
     5581 gagccgagat gaagatcctg atcgccgagg acgatccggt cgtcgcactc ggtctcaccg
     5641 agcgagtgcg ctcgctcgga cacgatccga tcggcccagt cggcgacggc gaggaggctg
     5701 tcgccgaagc gaaagcgagc ctccccgacc tctacctctt cgacatcgag atgcccaatc
     5761 tcgacgggct ggcagctgca gtgcagctgg ccggcgaggg cctccggcga ccggtcgtga
     5821 tcgtgaccgg cgtcgatgac ccgagcctcg tcgaacgttc gatcgcgagc ggcgtcagcg
     5881 cctacctcac caaaccgatc gacacgcgcg aactagaggc ggcaatcaag ctggcggccg
     5941 cgcgtcacgc cgagttcgaa gcgctcgagg ccgaagtgag caaggctcag caggcgctcg
     6001 aggaccgcaa gctcgtcgag cgcgcgaagg gcctgttgat gtcggcactc aagctctccg
     6061 aacaggacgc cttccgcaga cttcagatga ccgcacgcga gcgcaacctg cggctcgccg
     6121 atgtcgcccg ccgcatcgtc gagcagcaaa gcctgctcga cccgaagcaa gactcgccgg
     6181 cggcctctcc gtagcgccca ggcgtcccga tcgaatcagc ggacgctatt cgggtcggtg
     6241 agagaccgcc tcgatgttgt gtccgagcag acgcagatgc aaccgtggcc gcagacggcc
     6301 tacggaacct gcagcgagtg atcctggcgt tagacgggcg ccgcgtgctg aagttcacgg
     6361 acgcggaagg cgaggacgat gttcgtgaca ccgcgaatga ccgcgaaggc ggccacccag
     6421 gcgacgagga ggacggcgct gcgtccgtag tagccggccg cccagaagcc gagcgcaagc
     6481 tcgatgatgc ctccgatcag ttgcagccac cacacgtcga tctcacgccg cgcggaaatc
     6541 gccatgatga tgtcgaacgt cccggcgaac acgaggaaga agctgatcac cgctgcgagc
     6601 gcgaggaagg tatctccggg atggatgaac gagattatcc ccgtcgcgat gaagacgccc
     6661 gccaagactc cgtagagcag cttccaccag ccctgggcga gcatcgtcat tccgatctcg
     6721 aaaccgccgg ccgcgatcgc gatgacgccg aagaggatcg ctatcgcgtt cacggacgtg
     6781 tagtcaaagc gcaagacgat cagggcgacg accgtccagg cgataccggt gatcaggaac
     6841 agccaccacg gtggcagcac gcgcccgagc gtacgctggg cgatggcggg aatcgaggac
     6901 atcctttgtc tcccgaggtc gaggtcaccg agggtatatc gcagctccgg agaagggtca
     6961 atcgatcaag acctcagccg ctgggagaac cttcatgtcg agcggcgcgc cgctaatgcg
     7021 tgtaacggcc gcttcgatct cagctcctgg ccgctagcgg agcgagacgc gcgcgcggac
     7081 ctgctgtgcg agcttcgccg ggatccgcag aatcgtcgag tcccgcaaca ggaatgggcg
     7141 gcggcgcgcg atgctgagat gcgcggactc gtcggtccac gggctcgcga tgtcgcaggt
     7201 gaccttgatc ttgagaaagc cgccacgcgg ccagctgtac gtcggcttgc caaccgacca
     7261 gaggcgcaga tagctcgctg gatcctgcac ggctttgccg ccgaccatca ccgtcgaggg
     7321 tgaaggcact ggtgtcggct ggagcgtcgc cgcgatccgg tcgagtatcg cccgcgctcg
     7381 cgaatcgagc cgaagccagt gaatggcgcc gcgcggatcg accgttgcgg atgcagagtc
     7441 gccgacgaag cggaccatgt tcaggtcggg gacgaagtac gcggtgtgca actggccctg
     7501 ctggtaggac cagcgcagcg agtagaaggg cgcaggcgtc gccgcgggcg tccacagttc
     7561 gaccgaccca ccgccgaaga agagactgat cgccaacggc tcgccgtcgt ccatcccgat
     7621 cgtcgcgcac ccgttgctgc cgcacagctg gaacccggcg ggaggctcct tcgcctcggc
     7681 gcctgccgtg accaccaaga accacgccgt cagcagcgcc gcgatcgctg cccgcctcat
     7741 ctcaaccctc ctgctcgctt ggacaacgta caccggtcag cgccggaggg ttccaccggc
     7801 ccagcgcgat cggagctcga cgatctcgat cccgccgaac ccggaactgc ggctggcgcc
     7861 tattcagtca cgtccgctga gcccaattgc cacacgccga tgacatttcc tggtgggtcg
     7921 cggaatgtgg ctacccacag gtcaccctcg ggatatggcg ccgtaacaac cgcgccgccg
     7981 tgagcaacga ccttctcgag cgtctcgtcg acacgctcga caaagatgta cggacatacg
     8041 ccggcctcgc cggcgaccgg caggtcggtc atgaagtggc cgatgacatg cccggtgcca
     8101 tcgtcgaagc tcgggtcatc cgggtcaccg cgaaggctcc aaccgaaaac agcctcgtag
     8161 aaggccgccg accggcgtga gtcctcggcc ggaatccgca ggtaggagat acctccgacc
     8221 ctgaagacgc gatgttcgtc ttccgggccc gatgagacct ggtcgcctgg cacctcactc
     8281 accatagacc cccacatccg cgtgattgtg ctacggcggc cgttccggga cgctcgtggg
     8341 ggagccgaaa ctcccccacg aacgaattgt ctacgccgcg aggtcgagca cgggctccag
     8401 ctcctccgga gcctgctcga gctcgtcgcg cctgatgagc gtcagcgccg cgacgatgcc
     8461 gaccgccgcg atgccggcgc cgacgacgaa ggcgccggtg aacccgtgca cgagcgcgga
     8521 cggcagcgcg gatccgtccg cgagtgcatg cgaggtccgc gacgttgcga tcgtcgacag
     8581 cacggcgatg ccgagcgcgc cgccgatctg ctgcgtcgtg ttgaacagac cggatgcgag
     8641 gcccgcctcg gcgggccgga cgccggccag cgcggcgatg gagatcggca cgaaggagaa
     8701 gccgaggccg acgcccacga gcaagaagcc cggcagcagg tcgctgaaat acgagccgtg
     8761 gacggagacc tgcgtgaagt acacgagccc ggtcgtcagc gcaaccatcc cggtgacgag
     8821 cactggcttc acgccgatgc ggttcacgag ctgcgccgcg accgccgacc agaggatcgc
     8881 gcttccggcg actgcgagat acgccacgcc ggtcttcatg ggtgagtagc cgagcacctg
     8941 ctgcatgtac agcgtcagca tcaggaacag tg
//
LOCUS       DASVEU010000211         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1311096,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000211 DASVEU010000000
VERSION     DASVEU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311096"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF225_08985"
     CDS             complement(<1..429)
                     /locus_tag="VF225_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145425.1"
                     /translation="MRRPRAEHELPAAVVDVGWVNGLAAIRSLGRAGIRVLAVDHRPS
                     ALGFRSRYAEQVLGADPHTDPTRFIASIRALGKVVVFPTHDEQLNLIAQHLGDLEVLA
                     PFPGWDVLERVQSKRAQLEEAATVGVDVPRTRYPRRPADAR"
     gene            618..1457
                     /locus_tag="VF225_08990"
     CDS             618..1457
                     /locus_tag="VF225_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7145426.1"
                     /translation="MARIVVTGASGKAGRAVVRTLLEHDHDVLAVDVVSPTEPSAPSL
                     LADLTEYGQTVECLAGQDAVVHLAAIPASGIRTEETTFRTNMLSTYNVFEAARLVGLH
                     RVVWASSETILGLPFEREQPAYAPIDEDHPAYPESSYAVSKVLSEELGRQLYRWTGTP
                     YVALRFSNIMEPHDYESFPSYWNDPNLRRWNLWGYVDARDVAESCRLALEAEIGAEHF
                     IVAAADTVMNRPSRELMTEVYPAVPYQPTAGDFDTLLSIVKARKLLGYEPQWSWRDHI
                     AAT"
     gene            complement(1504..>2096)
                     /locus_tag="VF225_08995"
     CDS             complement(1504..>2096)
                     /locus_tag="VF225_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7145427.1"
                     /translation="DVVDLYQYHTPDNVTPFEETFGALDELVQAGKVRYVGHSNLKAA
                     QVEEVDAMCRERGFARPVSAQNQYSLLRREAEDELLPTCQRLGIGVLPFFPLASGLLT
                     GKYRRDEPRPPGTRLSDREGVFTDETFDRLEALEEFAQERRLTLLEVAIGGLLAQPAV
                     ASVIAGATKPEQVRANVAAAEWEPTFEDLAALNSLR"
BASE COUNT          356 a          734 c          664 g          342 t
ORIGIN      
        1 gcgcgcgtcc gcgggtctgc gcggatagcg cgttcgaggc acgtcgactc cgacggtcgc
       61 cgcttcttcc agttgggcgc gcttgctctg aacccgctcg aggacgtccc agcccgggaa
      121 aggagcgagc acctcgagat caccgaggtg ctgcgcgatc agattcagct gctcgtcgtg
      181 cgtcgggaac acgacgacct tcccgagcgc gcggatcgac gcgatgaacc gcgtcggatc
      241 ggtatgcggg tccgcgccga ggacctgttc cgcgtagcgc gagcgaaagc cgagcgcgct
      301 cggacggtga tccaccgcga gcacgcggat gcccgcgcgc ccgagcgagc ggatcgcagc
      361 cagcccgttg acccaaccca cgtcgacgac cgcagcggga agctcgtgtt cggctcgggg
      421 acgacgcatc gcgccgattc tgcccagccc taccgctccg acctccgatt gtcacgattc
      481 gggcacaccg gcgtgacacc tttcttgcga cactaaagaa gcggtttgca gccgttttgt
      541 cccatcgcga catgtcttgg ggtctgaccc aaagacagtg gccgaatgga acactcgact
      601 gctcgtaggc tttagccatg gcaagaatcg tcgtcacggg cgcgagcgga aaggccggac
      661 gggcagtcgt ccgcaccctg ctcgagcacg accacgacgt gctcgcggtc gacgtcgtct
      721 cgcccaccga gccgtccgca ccgtctctgc tcgccgacct gaccgagtac ggccagacgg
      781 tcgagtgcct ggcgggccag gatgccgtcg ttcaccttgc ggcgatccct gcttctggaa
      841 ttcgcactga ggagaccacc ttccggacga acatgctcag cacgtacaac gtcttcgagg
      901 cggcgcgact ggtcggccta caccgcgtcg tctgggcgtc gagcgagacg atcttaggcc
      961 tgcccttcga acgcgagcag ccggcatacg caccgattga cgaggatcac ccggcgtacc
     1021 cggaatccag ctacgcggtc tcgaaggtgc tctcggaaga gctcggccgc cagctgtacc
     1081 ggtggaccgg aacgccttat gtcgcgcttc ggttctcgaa catcatggag cctcacgact
     1141 acgagagctt tccgtcctac tggaacgacc cgaacctgcg ccgctggaat ctatggggct
     1201 atgtcgacgc gcgcgacgtc gccgagagtt gccgactcgc gctcgaggcg gagatcggcg
     1261 ccgagcactt catcgttgcc gccgcggaca ccgtcatgaa ccgcccgagc cgcgagttga
     1321 tgaccgaggt ctaccccgcg gtgccgtatc aacccacggc gggcgacttc gacacgctcc
     1381 tctcgatcgt gaaggcacgc aagctgctcg gctacgagcc gcagtggtcc tggcgggacc
     1441 acatcgccgc gacttagtgg ctcagagcca caatctctgc cgcctgatct cgcgcggcgg
     1501 ctactagcgg aggctgttca gggccgccag gtcctcgaag gtcggctccc actcggcagc
     1561 cgcgacgttc gcgcgcacct gctcgggctt cgtcgcgccc gcgatcacag acgcgaccgc
     1621 cggctgagcc agcaggccgc cgatcgcgac ctccagcaac gtgaggcggc gctcctgcgc
     1681 aaactcctcc agcgcctcga gccggtcgaa cgtctcgtcc gtgaagactc cttcacggtc
     1741 cgacagccgc gtgccaggcg ggcgcggctc atcccgtcgg tacttgccgg tcagcaaccc
     1801 actcgccagc gggaagaacg ggaggacgcc gatgccgagc cgctggcacg tcggcagcaa
     1861 ctcgtcctcc gcttcacggc gcaggagcga gtactggttc tgagcgctca cgggccgcgc
     1921 aaaaccgcgc tcgcggcaca tcgcgtcgac ctcttcgacc tgcgccgcct tcagattcga
     1981 gtgccccacg tagcgcacct tccctgcttg gacgagctcg tcgagcgcac cgaacgtctc
     2041 ctcgaacggc gtgacgttgt cgggagtgtg gtactggtag agatcgacca cgtcgg
//
LOCUS       DASVEU010000212        20518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_146268,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000212 DASVEU010000000
VERSION     DASVEU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 20518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20518
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146268"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2934)
                     /gene="dnaE"
                     /locus_tag="VF225_09000"
     CDS             complement(<1..2934)
                     /gene="dnaE"
                     /locus_tag="VF225_09000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7145428.1"
                     /translation="MEPPVREADQGRGFGGTGRFPRRTPTTSVPYVELHAHSSYSFLD
                     GASLPEELAVSAAELGYPALALTDHDGVYGSLEFAHAAKHFGVRPITGAELTLADRSH
                     VTVLVETAKGYANLCRLITAAHAETRPAGRESQPAADPALDQHLLEELNEGLVCLSGC
                     ARHGVAVHNPNGAARLARAFGPDRFFVELQRPYERGDARRNAGLRDLAEMLGVPTIVT
                     GDVHAHQLRRAPLQDVLVAISHRSALDGCEAERRGNHECVLLSPGEMVERFPEDRAAV
                     ARTVELAERLQFDLTEELGYRYPDFSDSPEPAIVQLAHVCNRTFEERYPGGHKLRREA
                     RVRLEDELKLIDELGLAGFFLLHWDVLELARECALEVRGRDSPRHALPPGRGRGSSVG
                     SLVCYLTGLSHVDPVGAELSLGRFLNRDLDSVPDIDLDFPRDIREKLIVAVTERYGRE
                     HAALVASFSTYRSRGAIRDVGKALGLPYAELERIARVSEGWNAKRIAEELQLLPDADR
                     KMMSPRWRAFAELCHEIAGLPRHISQHPGGMVISSRPLIELVPVQPAAMAGRQMCQWD
                     KDSCSDAGFLKIDLLGLGMLSAVEDCVDQIARLQGKPIDLSRIPLDDKAVYEEIQRAD
                     TVGDFQIESRAQMQSLLRTRPENMDDLTVQVALVRPGPIQGKAVHPYIEHRQRLREDP
                     SFVPPVDHPLLTDCLRSTLGVVVFQDQVLEVAIALAGFSVGEAEGLRRAMSRKRSHDA
                     LEAYRERFVAGALQKGVGLETADMVYDKLVGFSGFGFPKSHAAAFGLLAYQSAWLRHH
                     HPAEFLCALLNAQPMGFYPPATLVRDGQRRGVETRPPDVNISSAKCTVEEGAVRIGVD
                     YVNGIGEADAKAVVEEREREGSFQSVRDLAQRTQLSEHGLETLIVSGACDCFGMPRRK
                     LLWQLGLVPRSHSVPGSGGEEKQLALPLDPTAATPELPEPTVWERMLADYRTTNLSVG
                     VHPLEL"
     gene            3000..3758
                     /locus_tag="VF225_09005"
     CDS             3000..3758
                     /locus_tag="VF225_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145429.1"
                     /translation="MPGVVHKPGEGEALFNGRIVIKANFDELCITESLFPDARPGADP
                     HFHRQHADSFYVLEGGLAVLVDDEEKMLSPGACVCAPREVVHGFRSTSPTRFLNFHTP
                     DGGFARNLQERDRGEPGSFDSIDAPPGSGRPGSEAIFLNPGEGERLVGKNRIATIKIG
                     REELSLIEFELEPGFEGPDSHTHADHVDSFYVLEGEPEFLLDGERLRLGADSYVAAPI
                     GVVHAFSNPGPDRARLLNVHAPSRDFHESLRRMS"
     gene            3759..3983
                     /locus_tag="VF225_09010"
     CDS             3759..3983
                     /locus_tag="VF225_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7145430.1"
                     /translation="MTAGLVHLTLVPNEYEAELLCALLRTEGLTCDQRSTNLSVGRMD
                     GMPGGGPREIVVAEQDLARAQEILAASPND"
     gene            complement(4317..5501)
                     /locus_tag="VF225_09015"
     CDS             complement(4317..5501)
                     /locus_tag="VF225_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX7145431.1"
                     /translation="MIACLLIPGFELRAALRKQPSLALRPAALSPVAGTEPLLGPVTA
                     EAERLGVRPGMRLGEGLATCPELVLVEQDPAAAEQAWEEILRALEDTGFAVDPADVGC
                     VYFETRGVERLYGGLEPALRRALTAVGTAWDPRAGAAQRRFAALAAANVARPGQILIV
                     SDERLRDFLAPLPLELVPMEPRRRAELHELGVKRVGQLAGLPGSAVAERLGPDGRRAW
                     GLARGGKAARVRGRRPPAEIVEALEFPEAIGNELTLRRAFGALVETLMARPERGGRFV
                     RKLALSARLVGGGSWRRSATLRDPTTELDRIKAALGPKLAELPAPVVELRLELVELTE
                     HDGQQLELLPAAGGELDVRLREGLRQVRASTGSGSVNQVVEVAPWSRIPEARALFVPR
                     DD"
     gene            5806..6666
                     /locus_tag="VF225_09020"
     CDS             5806..6666
                     /locus_tag="VF225_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX7145432.1"
                     /translation="MRARALLALPLILVAAASAPRASAADAGVAALQVGLHSHGLYQG
                     PIDGLSGPRTLKAIRRLQKRAHITVDGVVGPQTRRALGRFARHRLGTRKLEIGNKGWD
                     VASLQFRLAEHGFPSGAFDGIFGRHINAALRRFQEWSALKTDGVAGPATLAALAAPPP
                     AIPLPLAWPMTAPVLGDRFGPRGDRWHSGIDLPAPMGAPVYAARSGRVVWAGWRDGGW
                     GFLVVVAHGHGERSMYAHLSRIDVRLGVWIGQGVRVGLIGATGDATGPHLHFEVRVRG
                     AAVDPLRALP"
     gene            complement(6699..6830)
                     /locus_tag="VF225_09025"
     CDS             complement(6699..6830)
                     /locus_tag="VF225_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145433.1"
                     /translation="MNEARAEWRRRLLLWNRRLVILLVAGFVLFSLVFTIVLMVMAR"
     gene            complement(6867..7997)
                     /locus_tag="VF225_09030"
     CDS             complement(6867..7997)
                     /locus_tag="VF225_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145434.1"
                     /translation="MRYTFLLAALTAALVLPASSGSAPRKPWLWQCTQIHNAEAQYHC
                     YVRLLRLDIETSRDPASELPRIDRRVAAVGGPVEAGCHVLMHEVGREFARDRRLTLAT
                     LQRYVPRSNNPNCSAGFGMGLVMYLGPQIIRSGGKSAVRTCASLPTRYREYTCVHGLG
                     HALLRAYHGDIGQAVRACRNLPRNFAPDCAQGVFHDYWISLRGADGTTAPRRTMSPRT
                     LCDGRLTYIRPCWYRYFLEQPVEQPVRNAADLRRSCRGLRSLERYGCISAAALTISSN
                     PFDQMRICAELRAADAGACLRGVPDQALAGHAAQQLRLIRDCASEAAGARSGCYEWLG
                     QTLAVVTNGLFRERGCTKLGPAPRRACSAGAARMRDALVTFS"
     gene            complement(7994..8668)
                     /locus_tag="VF225_09035"
     CDS             complement(7994..8668)
                     /locus_tag="VF225_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX7145435.1"
                     /translation="MRIDVAFTPVETAPAPIGLVVDVLRATSTIAQALASGYRRVLCC
                     AEIEDALALRAQIPDSLVGGERKAVQVEGFDVGASPREFLEPQADTLILSTTNGTRAI
                     LETAQRCEEVVLGSMLNLEAVARASADRDAVVVCAGFQGAFALDDAYCAGRIVQLVDG
                     ERTDAAIASELIARAFPTALEGFNARTYGPPGLEEDIAFCARENVLDVVPRLTGMVGP
                     AAEITA"
     gene            8689..9546
                     /locus_tag="VF225_09040"
     CDS             8689..9546
                     /locus_tag="VF225_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7145436.1"
                     /translation="MAEPVEQTCYRHPDRVTGLSCSECGRPICTECMTMAPVGIRCPD
                     HSGKAQGVQRVTKGVRRAAFEGTGAKVTKALIGINVAVYVAELAQGGGVYGVNSTIYQ
                     KGFLYAPFVGQGDWWRLMTAAFLHYGPFHLLLNMVGLYWFGSLLERRIGSGRFLLLYL
                     VSGLAGSAGALIVSPNSATVGASGAIFGILGAGLVLEQQRDYVFGGSALGVIVANLVL
                     TFAWSGNISVGGHIGGLIGGAAATLALSRFGRGHAAYGRAGLLGVAGIVAVGILSIGV
                     AYWKAKGYA"
     gene            9562..9855
                     /locus_tag="VF225_09045"
     CDS             9562..9855
                     /locus_tag="VF225_09045"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7145437.1"
                     /translation="MATVLVDAENVRRSLWPNMPGGELVQRSEAWGKQEGHDVRVVWE
                     GPESADDQIARLVEELEPRVWVVTSDRGLRDRVSDRAERIIGGGSFARELRRG"
     gene            complement(9852..10274)
                     /locus_tag="VF225_09050"
     CDS             complement(9852..10274)
                     /locus_tag="VF225_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145438.1"
                     /translation="MKRLTTLICLTIIAAVAGWGAGGSPAATFPTAAAKTCPAGYVHA
                     NLSWGEKCLRSGQYCKIKGDREYHRYRFHCHTGRLSRSASGGNQNGSGCQTGYSPCLP
                     RVADLNCSDIPESKKPIRVTGSDPYRLDGDGDGWGCEP"
     gene            10405..11166
                     /locus_tag="VF225_09055"
     CDS             10405..11166
                     /locus_tag="VF225_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7145439.1"
                     /translation="MARSLLALVSLVCLSAGAAALAPTTAVGRVVLDRPASPTGWVQV
                     PTPRKSAPAHVIAEVRPGHALALRSRPFGRVLAHVGAKTPFGSQRALSVVTTKHGRWL
                     AVTEAGVGNNRVVWIDAKSGGLRYARTRLELDVDLSARTLTVRRNGAAVRLLSVGVGR
                     AGSPTPTGTFAVTDKLNGAAYSAAYGCCILALSATQPNLPAGWSGGNRIAIHGTLSSS
                     DFGRAVSAGCVHASNSDLHYLMHTVPLGTPVVIRP"
     gene            complement(11153..12190)
                     /locus_tag="VF225_09060"
     CDS             complement(11153..12190)
                     /locus_tag="VF225_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7145440.1"
                     /translation="MHETSGIHAQVMAAAELSLAERVEGITQQDVRDILWERRQLVRA
                     WTLRGTLHLHPAGELPLWCAAARVVSPKDPEELQAWVDPKGELHPALGSADVQEIRAA
                     VLDALDGRCLLREELAAEVARRVGPKGQGRLLSGFMFFGADLCQGPPQGSKITLARPD
                     QWVESWKEVDEQECLHEVCRRYLRTYGPATPKDFRGWFSSKHFKPADARALFASLQLE
                     DVDVEGHTAHVLEGDTSFPEPQKTVRLLPEYDVYVMGFRERDQLVPEEARRQVAEHGR
                     GKYEGPAGVRFLMIDGVAAGLWERKKRGKRIELQVTPCRKLTRAERAEIDAQAERIGA
                     FLGLEPVLTVG"
     gene            complement(12285..12482)
                     /locus_tag="VF225_09065"
     CDS             complement(12285..12482)
                     /locus_tag="VF225_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145441.1"
                     /translation="MGYAILLDGERVAAMSSDEAVSAWIATYREDHAEDDPFAVHLQI
                     LERGALAWLTGGKLVDRERFL"
     gene            complement(12482..15094)
                     /gene="ppdK"
                     /locus_tag="VF225_09070"
     CDS             complement(12482..15094)
                     /gene="ppdK"
                     /locus_tag="VF225_09070"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7145442.1"
                     /translation="MAAERYVYAFDEPSTGGRDLLGGKGIGLAEMTRMGVPVPAGFTI
                     TTDACRAYMSNGKTLPDGVEQEIEEHLGALQEKTGKRFGDTNDPLLVSVRSGAAVSMP
                     GMMDSILNLGLNDDAVVGLARATENERFANDSYRRLIQMYGEVVDGIDGHRFEQALSD
                     LKKSRGASQDVDLSAHDLAGLIETYRAVYLEETGRDFPQDPHEQLGRAVRAVFDSWDS
                     PRAKVYRRTYDIPDELGTAVNVVQMVFGNKGDESGTGVAFTRDPSTGEPGLYGEFLAN
                     AQGEDVVAGIRTPEPLAHMEQKLPAAFAHLLDTMRRLEEHYRDMQDIEFTVEDNELYL
                     LQTRSAKRTAAAAVKAAVDMADEGLISREDAVARIDPAQLDQLLHPMIDPTADWEVAT
                     KGLNASPGAACGAIVLDADTAEERGKAGESVILVRWETTPDDIHGLIQAAGILTAHGG
                     MTSHAAVVARGMGKPCVAGCEGLSIDGDARTITVGDRTLSEGDLLTIDGGTGAVIVGE
                     VPLVAPEVNDDLQRILEWGDEHRTLKVRANADTPEDAAKAREFGAEGIGLCRTEHMFM
                     ADGRLPVVRQMILAEGEEGRRQALDKLLPMQQGDFEAIFEAMAGLPVTIRLLDPPLHE
                     FLPPAEQVTDERTRRRIKQLQESNPMLGTRGCRLGLQWPEIYEMQVRAIIRAARAVDQ
                     RTGEAPLVEIMHPLVGFEEELRRLRELTVRVAEEEAPKVEYLCGTMIELPRACIRADQ
                     IAEQADFFSFGTNDLTQTALGFSRDDAEGKFLTHYLEDGVLEQNPFETLDVDGVGDLM
                     RIAVERGRSTKPDLKLGICGEHGGEPKSVAFCHELGLDYVSCSPYRVPVARLAAAQAA
                     LGASGVVAYATAGG"
     gene            15135..15803
                     /locus_tag="VF225_09075"
     CDS             15135..15803
                     /locus_tag="VF225_09075"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX7145443.1"
                     /translation="MPEPTIVAMGGAPDETLLDYVLGLAPGRRLLYVPTAGMEDPART
                     VWWYERLHGRAEMTHLHFFPWPPSGLRERALDHDVILVTGGNTANALAVWRTHGFDEI
                     LREAWLEGILLTGWSAGMICWFEHGVTDSFGPELAPMECLGFLPGSACPHYDGEERRR
                     PVYKRLVAEGLAPGVAADDDVALHYVGSELREAVTSREGAAAYRVEADGESPLVTRLL
                     DPPR"
     gene            complement(16188..16352)
                     /locus_tag="VF225_09080"
     CDS             complement(16188..16352)
                     /locus_tag="VF225_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145444.1"
                     /translation="MKPKPPVPWWRWAVWWLILGFALVLFYILLTPVWLGLRGLAWLA
                     EFRARRRRGR"
     gene            complement(16349..17182)
                     /locus_tag="VF225_09085"
     CDS             complement(16349..17182)
                     /locus_tag="VF225_09085"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX7145445.1"
                     /translation="MGQPPVELHIVSDSTGETAARLVLALEAQFPEQAFEEVRHTRAE
                     TVDDLERAVASARGHAAVVIYTLVDPELRDAMRRLCRRARVHYCDLLGHPIDAVARVS
                     GQAARMTPGSRAPLTPAYFRRIEAMEFAVKYDDGVGAGLDEADIVLVGVSRTSKTPLS
                     IYLGYLGHKVANVPIVKGIEPPKELFELDPAKIVGLTIHADRLAEIRRARVRNLGANN
                     RRYAELTEVYDELDEATAVQRRLGCPVIDISELSIEETAQRILRTVEGRKAEAAAAEA
                     T"
     gene            17293..17685
                     /locus_tag="VF225_09090"
     CDS             17293..17685
                     /locus_tag="VF225_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145446.1"
                     /translation="MTPGGVRPLLALAVLLAALVFCGAGQAKAGCVSRLLADWRDGRI
                     DGTYSVACYRTALAHLPEDVRIYSSAESDIKRALVARSEVALAAPAKAKTQSRSDGNS
                     GNGVSPLLVLAVAGAILAALGSAFALAR"
     gene            17910..19265
                     /locus_tag="VF225_09095"
     CDS             17910..19265
                     /locus_tag="VF225_09095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7145447.1"
                     /translation="MSDDVTMDKIVSLSRRRGFVFPSSEIYGGMGSTFDYGHYGVLMK
                     NNIRERWFEAMVRNRDDIVAIDSAVILHPKVWEASGHVAGFTDPLIDCRTCKLRFRAD
                     KLEDSSCGRKPSKHPGETPECDLTEPRRFNLMFETTVGALQEDGSRAYLRPETAQGIF
                     INFKNVLQIARRKPPFGIAQIGKSFRNEITPGNFLFRVREFEQMEMEFFVPPDEAEKW
                     YRYWIDERLNWYRRYGLRDSKLRVREHGPEERSHYSQGTSDIEYDYPIGWSELEGIAN
                     RGDFDLTQHAEHSTTKLEYVGQDGERYVPYVIEPAVSIERIMVALLVDAYDEEVVGER
                     ERTVLRLHPKMAPVTAAILPLIGKSDEMVAKARSLYEDLRRRHSVEYDDGGAIGRRYR
                     RQDEIGTPWAFTVDEQTLSDDTVTVRDRDSLEQERLPIANVQDWVENALERDWKSPKG
                     D"
     gene            complement(19262..20011)
                     /gene="recO"
                     /locus_tag="VF225_09100"
     CDS             complement(19262..20011)
                     /gene="recO"
                     /locus_tag="VF225_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7145448.1"
                     /translation="MPGRSYKTEAVVLRSFRLGEADRVLHVYTLERGRIGAVAKGIRK
                     TKSRFGARLEPLSHVELMLYQGSSELQTVTGVELLRSHHESREQQYRLGVGLLGAEAM
                     LRLFTEQEGNPRAFQALTRFLDLLDELEPLTSRASLDPLALSFQLKLLWLSGYLPHLT
                     SCADCGTESETLVGYSAKAGGAVCSNCAAGGSLALSPAGIAGIEALLARPLAEARDAG
                     LTDRSTRDALTVITAAYEYFGNFRLRTLRSA"
     gene            20077..20331
                     /locus_tag="VF225_09105"
     CDS             20077..20331
                     /locus_tag="VF225_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX7145449.1"
                     /translation="MPRIRVYSTRWCGYCVRAKALLESRGIEYEEISLDDDPAFRQKL
                     FDLTGGWTVPQIIIDGRPIGGYTALWRLDRDGLLDDGLAA"
BASE COUNT         3337 a         7083 c         6791 g         3307 t
ORIGIN      
        1 gagctccaac ggatggacgc cgaccgagag gttcgtcgtg cggtagtcgg cgagcatgcg
       61 ttcccagacg gtcggctccg gcagctcggg tgtcgccgca gtgggatcga gcggaagcgc
      121 gagctgcttc tcctcaccgc ctgagccggg aacgctgtgc gagcgcggca cgaggccgag
      181 ctgccagagc agctttcgcc tgggcatacc gaagcagtcg caggcgccag agacgatcag
      241 cgtctcgagg ccgtgctccg acagctgcgt gcgttgcgca aggtcgcgca cgctctggaa
      301 cgacccctct cgctcgcgct cctcgacgac cgccttcgcg tccgcttcgc cgatgccgtt
      361 gacatagtcg acgccgatgc gcaccgcacc ctcctcgacc gtgcatttgg cagagctgat
      421 gttcacgtca gggggacgtg tttccacccc gcgccgctgg ccgtcgcgga cgagcgtcgc
      481 cggcggatag aagcccatcg gctgtgcgtt gaggagcgcg cacagaaatt ccgcggggtg
      541 gtggtgccgc aaccaggcgg attggtacgc gagcagcccg aacgccgctg cgtgcgactt
      601 cgggaacccg aaaccggaga agccgacgag cttgtcgtag accatgtccg cggtctccag
      661 gccgactccc ttctggagcg cacccgcgac gaaacgctcg cggtatgcct ccagcgcgtc
      721 gtgcgagcgc ttgcggctca ttgcgcggcg caggccttcc gcctccccca cgctgaagcc
      781 ggccagcgcg atcgccacct ccagcacctg atcctggaag acgacgacgc cgagcgtcga
      841 gcgcaaacag tccgtgagca gtgggtgatc caccggcggg acgaacgagg gatcctcgcg
      901 cagacgctgg cggtgctcga tgtacggatg gacggctttg ccctgaatcg ggccggggcg
      961 cacgagcgcg acctggacgg tgaggtcgtc catgttctcg ggccgggtgc gaagcaggct
     1021 ctgcatctgc gcgcggctct cgatctggaa gtcaccgacc gtgtcggcgc gctggatctc
     1081 ctcgtagacg gctttgtcat cgagagggat gcgggaaagg tcgatcggct tgccctgcag
     1141 ccttgcgatc tggtcgacgc agtcctcgac cgccgagagc atcccgagcc cgagcaggtc
     1201 gatcttgaga aagccggcgt cggaacagga gtccttgtcc cactggcaca tctgcctgcc
     1261 tgccatcgcg gccggttgca cgggaacgag ctcgatgagc ggccgcgacg agatgaccat
     1321 gccacccggg tgctgcgaga tgtggcgcgg caggccggcg atctcgtggc agagctcggc
     1381 gaatgcgcgc cagcgcggcg acatcatctt gcggtcggcg tccggcagca gctgcagctc
     1441 ctccgcgatg cgcttcgcgt tccagccttc cgatacgcgt gcgatgcgtt cgagctccgc
     1501 atacggaaga ccgagtgcct tcccgacgtc gcggatcgca ccacgggagc ggtacgtcga
     1561 gaacgaggca acgagtgctg cgtgctcgcg tccgtagcgt tcggtgactg cgacgatcag
     1621 cttttcgcgg atgtcacgcg ggaagtcgag gtcgatgtcc gggaccgaat cgaggtcgcg
     1681 gttgaggaaa cggccgagcg agagctccgc gccgacaggg tcgacgtgtg acaggccggt
     1741 gaggtaacag acgagcgagc cgaccgaaga gccgcgcccc cggcccggcg gtagcgcgtg
     1801 acgcggcgaa tcgcggccgc gcacctcgag cgcgcattcg cgcgcgagct cgagcacgtc
     1861 ccagtgcagg aggaagaagc cagcgagacc cagctcgtcg atgagcttca gctcgtcctc
     1921 gagccgcacg cgtgcctcgc ggcgaagctt gtggcccccc ggatagcgct cctcgaaggt
     1981 gcggttgcag acgtgcgcga gctggacgat cgccggctcc gggctgtccg agaagtccgg
     2041 gtagcggtag ccgagctcct ccgtcaggtc gaactgcaac cgctcggcga gctcgacggt
     2101 tcgcgcgacc gccgctcggt cctccgggaa acgctccacc atctcgcccg gtgaaagcag
     2161 gacgcattcg tgattgccac gcctctcggc ctcgcaaccg tcgagggcgc tgcggtgact
     2221 gatcgcgacg aggacgtcct gcagaggcgc ccggcgcagc tggtgcgcat ggacgtcgcc
     2281 cgtgacgatc gtcgggacgc cgagcatctc ggcgagatca cgcagaccag cgttgcgacg
     2341 cgcgtcgccg cgctcgtacg ggcgctggag ctcgacgaag aagcgatcgg ggccgaaggc
     2401 gcgggccaac cgtgccgcgc cgttggggtt atgcaccgcg actccatgtc gtgcacagcc
     2461 ggagaggcag acgagcccct cgttcaactc ctcgagcaaa tgttgatcga gtgcagggtc
     2521 cgccgcgggc tggctctctc ttcctgctgg ccgcgtctct gcatgagcag cagtgatcag
     2581 ccgacagagg ttcgcgtagc cctttgccgt ctcgacgagg acggtgacgt gcgagcggtc
     2641 ggcgagcgtc agctcagccc cggtgatggg acgcaccccg aagtgcttgg ccgcatgcgc
     2701 gaactcgagc gacccgtaga ccccgtcgtg gtcggtgagc gcaagcgcgg gatagccgag
     2761 ctcggcggcg ctgacggcga gctcctccgg cagggaggcg ccgtcgagga acgagtacga
     2821 cgaatgggcg tgcagctcga cgtacgggac ggaggtggtt ggtgtgcgtc gggggaacct
     2881 cccggttccc ccgaaccccc tcccctggtc cgcttcgcgg acaggcggct ccaccgcctc
     2941 tctcttccga gtccgatgtg gaaactcagg ttggagctcg accgtcatac tcgctcgaga
     3001 tgccaggtgt cgtgcacaag cccggcgagg gcgaagcgct cttcaacggg cggatcgtca
     3061 tcaaggcgaa cttcgacgag ctctgcatca ccgagtcgct cttcccggac gctcgccccg
     3121 gagcggatcc gcatttccac cggcagcacg cggattcgtt ctacgtcctc gaaggcgggc
     3181 tcgcggtcct cgtcgacgac gaggagaaga tgctgtctcc gggcgcgtgc gtctgcgccc
     3241 cgcgggaggt cgtgcatggc ttcaggagca cttctcctac gcggttcctg aacttccata
     3301 cgccggacgg tggcttcgca cggaatctgc aggagcgcga tcgcggcgag cccggcagtt
     3361 tcgacagcat cgacgcaccg cccggcagtg gccgccccgg atcggaagcc atcttcctca
     3421 atcccggcga aggcgaacgt ctcgtgggca agaaccggat cgcgacgatc aagatcgggc
     3481 gtgaggagct ctcgctgatc gagttcgagc tcgagcccgg cttcgaaggg cccgactcgc
     3541 acacacatgc cgatcacgtc gactcgttct acgtgctcga aggagagccc gagtttctcc
     3601 tcgacggcga gcggcttcgc ctcggagccg actcgtacgt cgccgcgccg atcggtgtcg
     3661 tccatgcgtt ctcgaatccc ggaccagacc gcgcacggct gctgaacgtg cacgctccga
     3721 gccgcgactt tcacgagtcc ctgcgacgga tgagctgaat gacggccggg ctcgtccacc
     3781 tgacgctcgt gccgaacgaa tacgaagcgg aattgctctg tgcgctgttg cgaaccgaag
     3841 ggctcacctg cgatcagcgg tcgacgaacc tcagcgtcgg aaggatggac ggcatgccgg
     3901 gcggtggccc gcgggaaatc gtcgtcgcag aacaggatct cgccagagct caggagatcc
     3961 tggctgcgtc tcccaacgac tgatcccccc tcacgcgccc cgcttaaccg tggaaaatgc
     4021 tgcgcatttt ccacgggaag ggtttgtccc ttcgcttcgc tcgggagcct ttgacaaagg
     4081 cggccgagct cacgcccccc gttgggtgaa ccaacaggct ttctcctcgt ctcgaaaaac
     4141 aacggcgttc tcccctgact gcaacacgac ttcgaagtag cgccgggaca ccggctcctc
     4201 cgtccaccac cgatccacta cgcgccactc ttcgcgaatc actgcgacag gcaagcgatt
     4261 cacccgcacc ggcgttccgt ccactcgggc ctcgacgacc gccgcccgag gctcgttcag
     4321 tcgtcccgcg gcacgaacaa cgcgcgcgct tccggaatgc gcgaccacgg cgcgacctcc
     4381 acgacctgat tgaccgatcc cgagccggtg ctggcgcgca cctgacgcag gccctcccgt
     4441 aaccgcacgt cgagctcccc acctgctgcg ggcagaagct cgagctgctg cccgtcgtgc
     4501 tcggtcagct ccacgagctc gagccgcagc tcgacaacgg gagcaggcag ctccgcgagc
     4561 ttcggcccga gcgcggcctt gatgcgatcg agctcggtgg tgggatcccg caacgtcgcg
     4621 cttcgtcgcc aggagccgcc gccgacgagc cgcgcggaca acgcgagctt gcgcacgaac
     4681 cgacccccac gctcggggcg cgccatcagc gtctcgacga gcgcgccgaa tgcgcgacgc
     4741 aacgtgagct cgttgccgat cgcctcggga aactcgagcg cttcgacgat ctccgccggc
     4801 gggcgccggc cgcgcacgcg cgcggccttc ccgcctctcg ccaggcccca ggcgcgccgg
     4861 ccgtccggcc ccaatcgttc ggccacggca ctgcctggta gcccagcaag ctgtccgacg
     4921 cgcttgaccc cgagctcgtg cagctccgcg cggcggcgcg gctccatcgg cacgagctcg
     4981 agcggcagcg gtgcgaggaa gtctcgcaac cgctcgtcgg agacgatcaa gatctgcccg
     5041 gggcgcgcga cgttcgctgc ggccaaagct gcgaaccggc gctgcgctgc gcccgcacgt
     5101 ggatcccagg cagtaccgac ggcggtgagc gcgcgtcgca gcgcaggctc cagtccgccg
     5161 tacaacctct cgaccccacg cgtctcgaag tagacacaac cgacgtcggc cgggtcgacc
     5221 gcaaacccgg tgtcttcgag cgcacgcaag atctcttccc atgcctgctc ggccgcggcc
     5281 gggtcctgct cgaccaggac gagctcggga cacgtcgcca gcccttcgcc gagccgcatc
     5341 cccggacgga cgccgagccg ctcggcctcg gccgtgaccg ggccgagcag aggctccgtt
     5401 ccggccaccg gcgacagcgc ggcgggtcgc agagcgaggc tcggctgctt gcgcaacgcc
     5461 gctcgcaact cgaagccggg gatgaggagg caggcgatca tgggaacgta tgttctacac
     5521 gaacacctgt tcgcctgtca agtaggtggt gagtatccct cctgtatagg cggccatcgt
     5581 tgggatagac cagcgcatat tgagccgctt ggcccattcc gatcccgttc caagggtgcc
     5641 atcgttgagc gtgacgccag gcgggggctt gcgatcaagg ttcgattgcg tggcggtcaa
     5701 cccacgactg cgccctcgcc tggcgtccca gttcttcatc gccttgagtc ggcggggccg
     5761 gtccagccaa gggatcccct cgggcacggc gaaaacggtc aacccatgcg tgccagggcg
     5821 ctcctcgctc tgcccctgat ccttgtcgcc gcggccagcg ccccccgggc ctcggcggcc
     5881 gatgcgggcg tcgccgccct ccaggtcggc ctgcactcgc atgggctcta tcagggcccg
     5941 atcgacgggc tcagcgggcc ccgtaccctg aaggcaattc gccgcctcca gaagcgtgcg
     6001 cacatcactg tcgacggcgt cgtcggcccg cagacccgca gggcgctcgg ccgcttcgcc
     6061 cgccaccgcc tcggcactcg caagctcgag atcggcaaca aaggctggga cgtcgcgtcg
     6121 ctgcagttcc ggctcgccga gcacggcttc ccctccggcg ccttcgacgg gatcttcggc
     6181 cggcacatca acgctgcact tcggcgcttc caggagtggt ccgcgctgaa gacggacggc
     6241 gtggccgggc ctgcgacgct cgccgcgctc gctgcaccgc cgcctgcgat tccgctcccg
     6301 ctcgcctggc cgatgaccgc gccggtgctc ggcgaccgct tcgggccgcg cggcgaccgc
     6361 tggcactcgg gcatcgacct ccccgcgccg atgggggcgc ccgtctacgc cgcgcggtcc
     6421 ggccgggtcg tctgggcggg atggcgcgac gggggctggg ggtttctcgt cgtcgtcgcc
     6481 catggacacg gcgagcgctc gatgtatgcg cacctttcac ggatcgacgt gcgcctcggc
     6541 gtgtggatcg gacagggagt ccgggtcggg ctaatcggag cgacgggcga cgcgacaggt
     6601 ccgcatctgc acttcgaggt gcgtgtccgc ggtgcagcgg tcgatcctct gcgtgcactt
     6661 ccctgaacgg ccggccgtcg tctgctcgcg ctgagcggtc atcgagccat gaccatcagc
     6721 acgatcgtga agacaagaga aaagaggaca aagccggcca caagcaggat cacaagccgg
     6781 cggttccaga gaagaagccg ccgacgccac tcagcacgtg cctcgttcat ccgggctcag
     6841 cttactccgg cagtccgcca aacctcctag gagaaggtca ccagggcgtc gcgcatacgc
     6901 gctgcgccgg ccgagcatgc ccgccgcggg gccgggccca gtttcgtgca ccctcgttcg
     6961 cgaaagaggc cgttcgtcac gacggcgagc gtctgtccca accactcgta gcaaccggac
     7021 cgcgcccccg cggcctcaga tgcgcagtcg cgaatcaggc gcaactgctg cgcggcatgc
     7081 ccggcgagcg cctggtcggg aactccgcgc aggcaggctc ccgcgtctgc cgcccgcagc
     7141 tctgcgcaga ttcgcatctg gtcgaacgga ttcgaggaga tcgtgagcgc agccgcgctg
     7201 atgcagccgt agcgttcgag ggaacgcagc ccgcggcagg aacggcgcag atccgctgca
     7261 ttgcgcacgg gctgctcaac cggctgttcc aggaagtacc ggtaccagca cggcctgatg
     7321 tatgtcagac gcccgtcaca cagggtgcgg ggagacatcg ttcgtcgcgg ggccgtcgtc
     7381 ccgtcagcgc ctctgagcga gatccagtag tcgtggaaca cgccctgcgc gcagtccgga
     7441 gcgaagttgc gcggcaggtt gcgacacgcg cgaaccgcct ggccgatgtc gccgtggtag
     7501 gcacgaagca acgcgtgacc gagcccgtgc acgcaggtgt actcccggta gcgagtcggc
     7561 aggctcgcgc aggtgcgcac cgccgacttg ccgccggacc ggatgatctg cggaccgagg
     7621 tacatcacga gccccatccc gaacccggcc gagcagttcg gattgttcga acgagggacg
     7681 tagcgctgca gcgtcgcgag cgtcaggcgg cgatcgcgcg cgaactccct ccccacctcg
     7741 tgcatgagca cgtggcagcc ggcttcgacc ggacctccga ccgctgctac acgccgatcg
     7801 atccgcggca actcgctcgc gggatcgcgc gacgtctcga tgtcgaggcg aagcaagcgg
     7861 acgtaacagt ggtactgcgc ctcggcattg tggatctgcg tgcattgcca gagccacggt
     7921 ttgcgtggag cgctgccact gctcgccggc aagacgagcg ccgccgtcag cgccgcgagc
     7981 aagaacgtgt acctcatgca gtgatctccg cagccggacc gaccattcct gtgaggcgag
     8041 gcacgacgtc aaggacgttc tcgcgggcac agaacgcaat gtcctcctcc agaccgggtg
     8101 gcccatacgt ccgcgcgttg aatccctcga gcgccgtcgg aaaggctcgg gcgatcagct
     8161 ccgaggcgat cgcggcgtcc gtgcgctctc cgtccaccaa ctgcacgatc cgccccgcgc
     8221 agtaagcgtc gtcgagtgcg aaggcgccct gaaagcccgc gcacacgacc acggcatccc
     8281 ggtcagccga ggcgcgtgct accgcttcga ggttcaacat gctccccaga acgacctcct
     8341 cgcagcgctg cgctgtctcc agaatcgccc gcgtcccgtt cgtcgtcgac aggatcaacg
     8401 tgtccgcctg cggctccagg aactcccgcg gcgatgcgcc gacgtcgaag ccctcgacct
     8461 ggacggcctt ccgctcccca ccgacgagcg agtcggggat ctgcgcacgc agcgccagcg
     8521 cgtcctcgat ctcggcacag cagagcacgc gccggtaacc ggacgcgagc gcctgtgcga
     8581 tcgtcgacgt cgcccggagc acgtccacca cgagcccgat cggggcagga gccgtctcga
     8641 cgggagtgaa agcgacgtcg atgcgcatct gactacgcta cttcgcgcat ggctgaaccc
     8701 gtcgaacaga cctgttaccg gcatcccgac cgggtgaccg gcctgtcctg ctcggaatgc
     8761 gggcggccca tctgcacgga gtgcatgacg atggctcccg tcgggatccg ctgccccgat
     8821 cactcgggca aggcgcaggg cgtgcagcgc gtgacgaagg gagtccgacg cgcggccttc
     8881 gagggcaccg gcgcgaaggt gacaaaggcg ctgatcggga tcaacgtcgc cgtgtacgtc
     8941 gcagagctcg cccagggcgg gggcgtctac ggggtcaaca gcacgatcta ccagaagggc
     9001 tttctctatg cgccgttcgt ggggcagggt gactggtggc ggctcatgac ggcagccttt
     9061 cttcattacg ggcccttcca cctgctcctg aacatggtcg gcctctactg gttcggttcg
     9121 ctcctcgagc gacggatcgg ctcaggccgg ttcctgctgc tctacctggt ttccgggctc
     9181 gcgggctcgg ccggcgcgct catcgtcagt cccaacagtg cgacggtcgg cgcgtcgggc
     9241 gcgatcttcg ggattctggg tgcgggcctc gtgttggagc aacagcgcga ctacgtcttc
     9301 ggaggcagcg cgctcggcgt gatcgtcgcc aatctcgtgc tcaccttcgc gtggagcggc
     9361 aacatctccg tgggcggtca catcggcggc ctcatcggcg gagcggcggc gaccctcgcg
     9421 ctctcccgct tcggacgcgg ccatgcagcc tacggacgcg ccggcctgct cggagtcgcc
     9481 ggcatcgtcg ccgtcgggat cctgtcgatc ggtgtggcgt actggaaggc gaagggctat
     9541 gcctaggcgg cgaggcccgg catggcgacg gtcctagtcg acgccgagaa cgtgcgccgc
     9601 tcgctctggc cgaacatgcc cggtggcgag ctcgtgcaac gcagtgaggc ctggggcaag
     9661 caggaaggac atgacgttcg cgtcgtctgg gaaggcccgg agtcggcaga cgaccagata
     9721 gcgcgtcttg tcgaagaact cgagccgcgg gtctgggtcg tcacgtcgga ccgaggtttg
     9781 cgggaccgcg tgagtgaccg cgcagagcgc atcatcggtg ggggctcgtt tgcgcgggag
     9841 ctgcgccgag gctagggctc gcagccccac ccgtcgccgt caccgtcgag acggtacgga
     9901 tcggagccgg tgacgcggat cggcttcttc gattccggga tgtcgctgca gttgaggtcc
     9961 gccacgcgcg gcaagcacgg cgagtaacca gtctggcaac cggaaccatt ttgattgcca
    10021 ccggacgccg atcgactgag ccgcccggtg tggcaatgga agcggtagcg gtgatattcg
    10081 cggtcgccct tgatcttgca gtactggccg gatctcaggc acttctcgcc ccagctcagg
    10141 ttggcgtgga catagccggc cgggcaagtc ttggctgcgg cagtaggaaa ggtcgccgcg
    10201 gggctcccac cggctcccca ccccgctact gcagcgatga ttgtgaggca gatgagggtg
    10261 gttagccgtt tcattccgtt tgctcccaac gttgactgct gtcatcctcg cacgtcgcgg
    10321 acgggccgca atgggccatc cggcccattc gaggcccctc gatcgaggga gccatgaccg
    10381 cccggacctg ccgatccaca ctgtgtggcc cggtcgctcc tcgcgctcgt ttcgctcgtc
    10441 tgcctcagcg caggcgcagc agcgctcgca ccgaccaccg ccgtcggacg cgtcgtgctc
    10501 gaccggcctg catcgcccac gggctgggtt caggtcccga cgccgcgcaa gtctgcaccc
    10561 gcgcacgtga tcgccgaggt ccggcccggg cacgcactcg cgctacgcag ccggccgttc
    10621 ggccgcgtcc tcgcgcacgt gggcgcgaag acaccgttcg gatcgcagcg cgcactcagc
    10681 gtcgtcacca ccaagcatgg ccgctggctg gccgtcaccg aggcaggtgt cggaaacaac
    10741 cgagtcgtct ggatcgacgc gaagtcaggc gggttgcgct acgcccgcac gcgtctcgag
    10801 ctggacgtcg atctttccgc acgcacactg accgtgcggc gcaacggcgc cgcggttcgc
    10861 ctgctcagtg tcggcgtggg tcgtgccggc tctccgactc ccactggaac cttcgcggtc
    10921 accgacaagc tcaacggggc cgcctacagc gctgcctacg gctgttgcat cctcgcgctt
    10981 tccgcgacgc agccgaatct gccggccggc tggagcggag ggaaccggat cgcgatccac
    11041 ggcacgctct cgtcgagcga cttcggtcgc gccgtgtctg cgggctgcgt gcacgcgagc
    11101 aattccgatc tgcattacct gatgcacacc gtcccgctcg ggacgccggt cgtcatccga
    11161 ccgtaagtac cggctcgagc ccaaggaagg caccgatgcg ttcggcctgg gcgtcgatct
    11221 ccgcacgttc ggcacgcgtg agcttcctgc acggcgtcac ctgaagctcg atgcgcttgc
    11281 ctcgcttctt ccgctcccag agccccgccg caacgccgtc gatcatcaga aaccgcacgc
    11341 cggcaggccc ttcgtacttg ccgcgcccgt gctccgccac ctggcgccgc gcctcctcgg
    11401 gcacgagctg gtcgcgctcg cggaagccca tcacatagac gtcgtactcg ggcagaagtc
    11461 gaacggtctt ctggggctcc ggaaaggatg tgtcgccctc gaggacgtgc gccgtgtgac
    11521 cttccacgtc cacgtcctcg agttgcagag aagcgaacaa cgcccttgca tcagccggtt
    11581 tgaaatgctt cgaagagaac cacccccgga agtccttcgg cgtagccggg ccgtacgtcc
    11641 gcaggtagcg gcggcagacc tcgtgcaggc actcctgctc gtcgacctct ttccaggact
    11701 cgacccactg atccggacgg gcgagcgtga tcttgcttcc ctgcggagga ccctgacaga
    11761 ggtcggcgcc gaagaacatg aagcccgaga gcaggcgccc ttgtcccttc ggaccgacgc
    11821 gtcgggcgac ctcggcggcc agttcttcgc gcagcaggca gcggccgtcg agcgcgtcca
    11881 agaccgcggc gcgtatctct tgcacgtcag cggaaccgag cgcaggatga agttcaccct
    11941 tcggatcgac ccaggcctgc agctcttcgg gatccttcgg tgacaccacc cgggcggccg
    12001 cgcaccacag gggcagttcg ccggccgggt gcaggtggag agtcccgcgc aaagtccatg
    12061 cccgcacgag ctgccggcgc tcccagagga tgtcacggac gtcctgctgc gtgatccctt
    12121 cgacccgttc cgcgagcgaa agctcggcgg ctgccatcac ctgcgcatgg atgccgctcg
    12181 tttcgtgcac gacgtcgaga agtttgtctg cacatgcgcg gtcggcaagt gagctgcgcg
    12241 caaggcgccg cgcacgaacc tcgttccagg aaacgttctt caggctagag gaaccgctcc
    12301 cggtcgacga gcttgccgcc ggtgagccac gcgagcgcac cgcgttcgag gatctggaga
    12361 tgcacggcga aaggatcgtc ctccgcatga tcttcgcggt acgtcgcgat ccaggcgctg
    12421 accgcttcgt cggagctcat ggccgcaacg cgctcgccgt caaggaggat ggcgtaaccc
    12481 atcagccgcc cgcggttgcg tacgcgacga caccgctggc gccgagcgca gcctgggccg
    12541 ccgcgagccg tgcgaccggc acgcgatagg gagaacagga cacgtagtcg agccccagct
    12601 cgtggcagaa ggccaccgac ttcggctctc ccccgtgctc gccgcagatg ccaagcttca
    12661 ggtcgggctt cgtcgaacgt ccgcgctcta ccgcgatgcg catcaggtcg ccgacaccgt
    12721 cgacgtcgag cgtctcgaac gggttctgct ccagcacgcc gtcctcgaga tagtgcgtca
    12781 agaatttgcc ctcggcgtcg tcgcgcgaaa agccgagcgc tgtctgcgtg aggtcgttcg
    12841 ttccgaaaga gaagaagtcg gcctgctcgg cgatctggtc ggcgcggatg cacgcccgcg
    12901 gcagctcgat catcgtcccg cagagatact cgaccttcgg cgcttcctcc tcggcgacgc
    12961 gcacggtcag ctcacgcaga cggcgcagct cctcctcgaa cccgacgagc gggtgcatga
    13021 tctcgacgag gggcgcctca cccgtgcgct ggtccaccgc gcgcgcggcg cggatgatcg
    13081 cgcgaacctg catctcgtag atctccggcc actgtaggcc gagccggcac ccacgggtcc
    13141 ccagcatcgg gttcgactcc tgcagttgct tgatgcgccg gcgcgttcgc tcgtcggtca
    13201 cctgctcggc aggcggcagg aactcgtgca gcggtggatc gagcaaacga atggtcacgg
    13261 gcaggcccgc catcgcctcg aagatggcct cgaagtcgcc ctgctgcatc ggcagcagct
    13321 tgtcgagcgc ctgacgccgg ccctcttcgc cttcggccag gatcatctgg cgcacgaccg
    13381 gcagccggcc gtcggccatg aacatgtgct ctgtccgaca gagcccgatt ccctcggctc
    13441 cgaactcacg cgccttcgcc gcgtcctcgg gcgtgtcggc attcgcgcgc accttcaggg
    13501 tgcggtgctc gtccccccat tcgaggatcc tctggaggtc gtcgttcacc tcgggcgcca
    13561 cgagcggcac ctcgccgacg atcaccgctc cggtgccgcc gtcgatcgtg agcaggtcgc
    13621 cctcggacag agttcggtcg ccgacggtga tcgtgcgtgc atcgccgtcg atcgagagcc
    13681 cttcgcagcc ggccacacac ggcttgccca tgccacgtgc caccactgcg gcgtgtgagg
    13741 tcatgccgcc gtgcgcggtg aggattcccg ccgcctgaat cagaccgtgg atatcgtcgg
    13801 gcgtcgtttc ccagcggacg aggatgacgc tctcgcctgc cttcccgcgc tcctcagccg
    13861 tgtcggcgtc gagcacgatc gctccgcagg ctgcgcccgg cgaggcattc aagcccttcg
    13921 tggccacctc ccagtccgcg gtcgggtcga tcatcggatg cagcaactgg tcgagctgcg
    13981 cgggatcgat ccgcgccacg gcgtcctcgc gcgagatcaa tccttcgtcc gccatgtcga
    14041 ccgccgcctt gaccgccgcc gcggcggtgc gcttggcaga gcgcgtctgg aggagataga
    14101 gctcgttgtc ctcgaccgtg aactcgatgt cctgcatgtc gcggtagtgc tcctccagcc
    14161 gacgcatcgt gtcgagtaga tgcgcgaagg cagccggaag cttttgttcc atgtgcgcga
    14221 gcggctccgg cgtacgaatg ccggcgacga cgtcttcgcc ctgcgcgttc gccaggaact
    14281 ccccgtatag ccccggttcg ccggttgagg gatcgcgcgt gaacgcaacc ccggtgccgg
    14341 attcatcgcc cttgttcccg aagaccatct gcacgacgtt gaccgccgtg ccgagctcgt
    14401 ccggaatgtc ataggtccgg cgatagacct tcgcgcgcgg cgagtcccac gaatcgaaga
    14461 ctgcgcgtac cgcgcgcccg agttgctcat ggggatcctg cgggaagtcg cgaccggtct
    14521 cctccagata gaccgcgcga tacgtctcga tcaatccggc cagatcgtgg gccgacagat
    14581 cgacgtcctg cgaggcgccg cgggacttct tcagatcgga gagcgcctgc tcgaaccggt
    14641 gcccgtcgat gccgtcgacg acctcgccgt acatctggat cagccgccgg taggagtcgt
    14701 ttgcgaatcg ctcgttttcg gtggcgcgtg cgaggccgac aaccgcgtcg tcgttgagcc
    14761 cgaggttgag gatcgaatcc atcatcccgg gcatcgagac cgccgcgccc gaccgtacgg
    14821 acacgagcag cgggtcgttc gtgtctccga agcgcttgcc cgttttctcc tggagcgcgc
    14881 cgagatgttc ctcgatctcc tgctccaccc cgtcgggcag cgtcttgccg ttcgacatgt
    14941 acgcacggca ggcatcggtc gtgatcgtga agccggccgg gaccgggacg cccatccgcg
    15001 tcatctccgc aagacctatt cccttgccgc cgagtaggtc acgccctccg gtggaaggct
    15061 cgtcgaaggc gtagacgtag cgctcggcgg ccaagggcga atcctctcac aaatgccctg
    15121 caatactcgg cgttatgccg gagcccacga tcgtggcgat gggcggagca cctgacgaaa
    15181 cgctcctcga ctacgtgctc ggcctcgcgc ctggaaggcg actgctctac gtgccgaccg
    15241 ccggaatgga ggacccggca cggacggtct ggtggtacga gcgactgcac gggcgtgcag
    15301 agatgacgca cctgcacttc ttcccctggc caccctcggg actacgcgag cgggcgctcg
    15361 accacgacgt catcctcgtc acgggcggca acaccgcgaa cgctctcgcg gtctggcgca
    15421 ctcacggctt cgacgagatc ctccgcgaag cctggctgga aggcattctg ttgacgggct
    15481 ggagcgcggg gatgatctgc tggttcgagc acggcgtcac ggattcgttc gggcccgagc
    15541 tcgcgccgat ggagtgcctc ggctttcttc cgggcagcgc gtgcccccac tacgacggtg
    15601 aggaacggcg ccgtcccgtc tacaaacggc tcgtcgccga aggattggcg cccggcgtcg
    15661 ccgccgacga cgacgtggcc ctgcactacg tcggctccga gctccgcgag gccgtcacct
    15721 cgcgcgaggg agccgccgcg taccgcgtcg aggcagacgg cgaaagcccg ctcgtgacgc
    15781 ggctgcttga tcctcctcgc tagatcagcc tcggcagcgg cgtccgatac cgaagcggac
    15841 ggtctcgctc cgcctgccga cgtggaggac gagcggcaga caggccgatg gcgagcggag
    15901 ataaaagccg cccgagaacg cgaggccacg tcccggggga ccagggcaac ccgcaatccg
    15961 gagagtgtcg cacgaggcgg taccgatact gcggacgacg gtcgccgacg tagggaaacc
    16021 tcgtggtgtc gatgttttcc ccgcagggaa cagttcgccc gggggtcgac gacgcagtgc
    16081 tcgccatcgc gccggccccg taggcgaatc cagctgtcac gcagagaatc gcgattcggc
    16141 gcggcgtcac gtgcctggta cgcggctgac gcgtcacggg ttccatttca gcgcccgcgc
    16201 cggcgccggg cgcggaactc cgccagccag gcaagccctc tgagccccaa ccagaccggc
    16261 gtcagcagga tgtagaagag gacgagcgcg aagccgagga tgagccacca gacggcccag
    16321 cgccaccagg gaacgggcgg cttcggcttc acgtggcctc cgctgccgcc gcctccgcct
    16381 tccggccctc caccgtgcgc aggatccgct gcgccgtctc ctcgatcgac agctccgaga
    16441 tgtcgatcac ggggcagccg agcctccgtt gcaccgcagt cgcctcgtcg agctcgtcgt
    16501 acacctcggt cagctcggcg tagcggcggt tgttggcgcc gagattccgc acccgcgcgc
    16561 gccggatctc ggccagccgg tcggcgtgga tcgtgaggcc gacgatcttc gcgggatcca
    16621 gctcgaacag ctccttcggc ggctcgatcc ccttcacaat cggcacgttg gcgaccttgt
    16681 gaccgagata gccgaggtag atcgacaacg gcgtcttcga ggtgcgcgag acgccgacga
    16741 ggacgatgtc ggcctcgtcg agcccagccc ccactccgtc gtcgtacttg accgcgaatt
    16801 ccatcgcctc gatgcgtcgg aagtacgcag gagtcagcgg tgcccgggag cccggcgtca
    16861 tacgggcggc ctggccggac acgcgtgcga cggcgtcgat cggatgcccg agcaggtcgc
    16921 agtaatgaac ccgggcgcgg cggcagagcc ggcgcatcgc atcgcgcaac tcagggtcga
    16981 cgagcgtgta gatgacgacg gcggcgtgtc cgcgcgcgct ggcgaccgcc cgctccagat
    17041 cgtcgacggt ctccgctctc gtgtgacgca cctcctcgaa ggcctgctcg ggaaattgcg
    17101 cctcgagcgc cagcaccagg cgcgcggccg tctctcccgt cgagtcggag acgatgtgga
    17161 gctcgaccgg aggctggccc atcccgagag aggctagtgg ctagaggcac gcgcgagtga
    17221 ccagcgcacc tattcacaaa gcctttaccc gattctgagt gagttcttag agatcggggg
    17281 aaccctcttg agatgactcc tggaggcgtc aggccacttc ttgccctcgc ggtcctactc
    17341 gcggcgctcg ttttctgcgg ggccggccaa gcaaaggccg gctgcgtctc gaggcttctc
    17401 gccgactggc gcgacggccg catcgacggg acctactcgg ttgcctgcta tcgaaccgcc
    17461 ctcgcccacc tgccggagga tgtccggatc tactcgagcg cagagagcga tatcaagcgc
    17521 gcgttggtcg cccgcagcga ggtggccctg gcggccccgg caaaagccaa aacgcagagt
    17581 cgatccgacg gcaacagcgg caacggcgtg tctccgctcc tcgtgttggc agtcgccggc
    17641 gcgatcctcg cggcactggg ctccgccttc gcgctcgcgc ggtagagcag gcagacaact
    17701 cggataacgt gccgcggcat gcccgtcgct cgcctccggc tcgctcccgt gagggaaacc
    17761 atgtttccct cacgagctcc cttctttagg gatcgcctca gcgcccgcgg cgtggcgacc
    17821 gccgccgcgc gcactgtgga gaaagagtgg ggaaacctcc cggtttcccc ttatgcccct
    17881 tccaccgctc atcggccaca gagccgccga tgagcgacga cgtcacgatg gacaagatcg
    17941 tctcgctctc ccggcggcgc gggttcgtct tcccttcctc cgagatctac ggcggcatgg
    18001 gctcgacctt cgactacggc cactacggcg tcctgatgaa gaacaacatc cgcgagcggt
    18061 ggttcgaggc catggttcga aaccgcgacg acatcgtcgc gatcgactcg gcggtcatcc
    18121 tgcaccccaa ggtctgggag gcatccggcc atgtcgccgg cttcaccgac ccgctcatcg
    18181 actgccgcac ctgcaagctc cgctttcggg cagacaagct cgaggattcg agctgcggcc
    18241 gcaagcccag caagcacccg ggcgagaccc ccgagtgcga cctgaccgag ccgcgccggt
    18301 tcaacctgat gttcgagacg accgtcggcg cgttgcagga ggacggctcg cgggcgtacc
    18361 tgcggcccga gaccgcccag ggcatcttca tcaacttcaa gaacgtgctc cagatcgcgc
    18421 gacgcaagcc gccgttcggg atcgcccaga tcggcaagtc gttccgcaac gagatcacac
    18481 ccgggaactt cctcttccgc gtccgggagt tcgagcagat ggagatggag ttcttcgttc
    18541 cgccggacga ggcggagaag tggtaccgct actggatcga cgaacggctc aactggtacc
    18601 ggcgctacgg gctccgcgac agcaagctgc gggtacgcga acacgggccg gaggaacgtt
    18661 cgcactactc gcagggaacc agcgacatcg agtacgacta tccgatcggc tggtcggagc
    18721 tcgaagggat cgcgaaccgg ggtgacttcg acctgaccca gcatgccgag cattcgacga
    18781 cgaagctcga gtacgtggga caggacggcg agcgctatgt cccttacgtg atcgagccgg
    18841 ccgtcagcat cgagcggatc atggtggcgc tgctcgtcga tgcctacgac gaggaggtcg
    18901 tcggcgagcg cgaacggacc gtgctgcgcc tccatccgaa gatggctccg gttaccgcgg
    18961 cgatcctgcc gctcatcggc aagagcgacg agatggttgc gaaggcgcgc tctctctacg
    19021 aggacttgcg ccgccgccac agcgtcgagt acgacgacgg cggcgcgatc ggccgccgtt
    19081 accgtcgtca ggacgagatc ggcacgccgt gggccttcac cgtcgacgag caaacgctgt
    19141 cggacgacac ggtcacggtc cgcgaccgcg attctctcga gcaagaacgg ctcccgatcg
    19201 cgaacgtcca ggactgggtc gagaacgcgc tcgagcgcga ctggaagtct ccgaaaggcg
    19261 attaagcgct gcggagggtg cgcagcctga agttcccgaa gtattcgtag gcggcggtga
    19321 tgacggtgag cgcgtcacgc gtcgaacggt ccgtgaggcc cgcgtcccgc gcttctgcga
    19381 gcggacgcgc aagcagggct tcgatccccg caataccggc aggcgagagc gccagggagc
    19441 cgccggcggc acagttgctg cacacggcgc cccccgcctt cgccgagtag ccaacgagag
    19501 tctcgctctc ggtgccgcag tcggcgcaac tcgtcaggtg cgggaggtaa ccggacagcc
    19561 agaggagctt gagctggaac gagagcgcga gcgggtccag cgatgcgcga ctggtgagcg
    19621 gctcgagctc gtcgaggaga tcgaggaaac gggtcagtgc ttgaaacgcg cgcgggttgc
    19681 cctcctgttc ggtgaagagg cgcagcatgg cctccgcgcc gagcagcccg accccgagtc
    19741 ggtattgctg ctcacgggac tcgtggtggg agcggagtag ctcgactccc gtgaccgtct
    19801 gtagctcgct cgagccctga tacagcatca actcgacgtg cgagagcggt tcgagccgcg
    19861 cgccgaaccg cgacttcgtc ttgcggatcc ccttcgcgac ggcaccgatt cgtccgcgct
    19921 cgagcgtgta gacgtgcagc acgcgatcgg cttcgccgag ccggaacgag cgcagcacga
    19981 ccgcctcggt cttgtaggag cgtccgggca tggctcggcc agtctagtcc cggcggtgga
    20041 gggaaccttt gcgggccttt cttcgttagg aatcaaatgc ccaggattcg cgtctattcg
    20101 actcgctggt gcggctattg cgtccgcgcg aaggccctgc tcgagagcag aggcatcgag
    20161 tacgaggaga tctcgctcga tgacgacccg gccttcagac agaagctctt cgatctcacc
    20221 ggcggctgga ccgttccgca gatcatcatc gacggccggc cgatcggcgg ctacacggcg
    20281 ctgtggcgcc tcgaccggga cggcctgctc gacgacgggc tcgccgcctg acgttccagg
    20341 cgctgacagt tcgggcagta ccacgtgccg cgacccgcgg tgcggatctt gtcgatcggc
    20401 gtcccgcagc gcttgcaggg acgtccttca cgtccataga cccgaaagcg gtcctgcatt
    20461 cgaccccttc caccgtcggg ggtcgagtaa tcgcgcagac tcgcaccctg gcgtttga
//
LOCUS       DASVEU010000213        12575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_147702,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000213 DASVEU010000000
VERSION     DASVEU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147702"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VF225_09110"
     CDS             complement(<1..932)
                     /locus_tag="VF225_09110"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX7145450.1"
                     /translation="MIDLRSDTLTQPTPAMRAAIANAAVGDDQKREDPTVNELERRGA
                     EFLGQEEAVFVPTATMANQIALNILGRPGDALLVERHAHIMLSELGGPAAHSGLLTIG
                     LEGTKGRFSPGQVVSEIRDRTSVHVAPTRVVTIENTHNSAGGRAWPLDEVDAIVASCR
                     EHDLAVHLDGARLVNAAVATGVDSARIGRGFDTVTLCFSKGLGCPLGALIAGSAELMA
                     QARRGKHYFGGAMRQAGIVAAAALYALDHHVDRIADDHARARRLAEGLAEAGVGVDLD
                     QVETNFVQIDVGPDRGGAIERMKERGVLVSTTVHP"
     gene            1021..1551
                     /locus_tag="VF225_09115"
     CDS             1021..1551
                     /locus_tag="VF225_09115"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145451.1"
                     /translation="MNPSDKRYVGGGLRTASGSAVPLRLDHGYFEHEVRPMLGSILVQ
                     KGWISHERLDAALAEVKVTGMRLGETLLARGWLFEPELASALAEQAGLRYVDLVRHPL
                     DPSAARLLPLEVARRMFAVPVRFLESGRIEVAVGDPADVDVDLLERVLGRGVDLVVGE
                     RSAIQDAWRYAPSCAK"
     gene            1594..2598
                     /gene="rlmN"
                     /locus_tag="VF225_09120"
     CDS             1594..2598
                     /gene="rlmN"
                     /locus_tag="VF225_09120"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7145452.1"
                     /translation="MGVMDRTLLQQRLSERGEAAYRAGQVWEWAARGAAGYDEMTNIP
                     GSLRAELEDEVPFSTLELVHEAKASDGTVKALFRTGEDHPVEAVLMRYRDGRRTVCLS
                     SQSGCPLTCAFCATGQMRFGRNLTASEILDQALYFRRIEPIDHAVFMGMGEPMLNLDS
                     VLEAARALPDLGITWRRTTISTVGWLPGLRRFVDEVEEPIRLAFSLHAPTDELRSELM
                     PVNDRYPLAAVIAECTRYFAVHRRKVFVEYVMLRGVNDRVEQARALTDLLDPKVFKVN
                     LIPYNPTGMYDGSTSKAIAAFKAALDRARLPATVRLTRGRDIQAACGQLAVVEAKGQL
                     "
     gene            2599..3321
                     /locus_tag="VF225_09125"
     CDS             2599..3321
                     /locus_tag="VF225_09125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7145453.1"
                     /translation="MAWPWEVVERDHEIQNPTSPEKIRVLGEYLRLTSESRVLDIACG
                     KGGPALILAATYGCRVHGVEIRPAFAEVARARAAANGLESLIEVQTADASELEFEPES
                     CDAALCIGASFVWGTIADAAAALLPFVRAGGFVAIGEPFWRQWPLPDGIEEEDFVDLH
                     ATVERFQRAGLASTGVIAASEDDWDNYESLHWRATEEWLAEHPDHPDAEDIRTQHSRY
                     RSDYFRFKRALLGWAIFVGRKV"
     gene            complement(3412..3735)
                     /locus_tag="VF225_09130"
     CDS             complement(3412..3735)
                     /locus_tag="VF225_09130"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX7145454.1"
                     /translation="MNKIQSASGRITEEVTSWPGVTAGPGRRGEFAFKVGGREIGHLH
                     GDRAAHFGFPKQVWRELKEQGRIEPHPVFPDAEGPAARLIVDDGDVRDVIELLRLNYD
                     RAVRR"
     gene            complement(3732..3923)
                     /locus_tag="VF225_09135"
     CDS             complement(3732..3923)
                     /locus_tag="VF225_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145455.1"
                     /translation="MAGEEGFSDGFRKLDDLVLTLKGLVLVRSVRERRGADDDELLMY
                     GAEIERVRSKLARFARSGE"
     gene            complement(3923..5398)
                     /locus_tag="VF225_09140"
     CDS             complement(3923..5398)
                     /locus_tag="VF225_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7145456.1"
                     /translation="MDILTKSERRWSALALIVTAQFMVVLDVAIVNVALPSIKTDLNF
                     SQANLQWVISAYAILFGGALLLGGRLADLLGRRRLFVAGLALFTTSSLLCGLAWSESS
                     LIAFRALQGLGGALLAPAALSLLMTTFAEGRERNLALGIYGAASGSGAAAGVLLGGVL
                     TSYLSWSWVFFINIPVGVAAIVLTPLLLRESRAGLANRHFDVPGAASITGGLMLLVYA
                     TTRATTDGWGSGTTIALLAGAAGLVLAFIAIELRSPSPLLPLRIFRVRSLTAANAAMA
                     IVGSVAFSEFFLLTLYLQDVLHYSAMQSGAAFVAFASAVVVASNVAQVVVGRFGVKPT
                     LTAGLVASAVSVAALTRLPVDGHYFWDLFPWFVLGGAGMGLSFVPITIASLTGVERSD
                     AGVASGLINTSRQVGGAIGLAAVSAIAATSTSNYAHVHSALTPSSSVALDHGFQTGLY
                     VLTGLLVIGAVIAATLVKSAPALGADEPAVEGEPIALEEAA"
     gene            5473..5982
                     /locus_tag="VF225_09145"
     CDS             5473..5982
                     /locus_tag="VF225_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7145457.1"
                     /translation="MALTTPETQPRTVPQLPGELVASATFLLKKLGFAAKDRTMKAYE
                     GTGLHPYHHAILLVLDVGSAETQGSIADALGYDRGQLVGLLDELEEQGLIERRRDPDD
                     RRRHLVRLTSDGKRALKRLRALSKEIEDEFLSPLGDKERANLHALLLRLAEKHEPRCA
                     LSVPLPPTR"
     gene            6022..6654
                     /locus_tag="VF225_09150"
     CDS             6022..6654
                     /locus_tag="VF225_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145458.1"
                     /translation="MIGKWFESYDEAWSYFLARETPLEDFWSVLPKPEGSILAGWLIE
                     PPSQAKDAVHDLLARLGKVEGVAVVPSHFLHIWIHGVAHDPTADRMSELVDEAREAWA
                     RRGSCEITLRHANCFHDAAVIEAHTDGVPRLLAALNPDADLRVALPHMTVAYLRSPSA
                     ASVRDVLGDFRGQEFGSFHADNVRLCLIPFARSTFMRPWKVAATHPLSSA"
     gene            complement(6682..7176)
                     /gene="msrA"
                     /locus_tag="VF225_09155"
     CDS             complement(6682..7176)
                     /gene="msrA"
                     /locus_tag="VF225_09155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7145459.1"
                     /translation="MEKKATFGAGCFWGVEAAFRQLEGVTRTRVGYSGGTTENPTYED
                     VCSHTTGHAEVVEVTYDPDRISYEQLLDVFWAKHDPTQLNRQGWDIGDNYRSAIFFHD
                     EEQHEAALKSKAGEQRNWGAPIVTQVEPAQAFYEAEVYHQQYLEKRGRSSCTPALQRA
                     TAAS"
     gene            complement(7176..7571)
                     /gene="msrB"
                     /locus_tag="VF225_09160"
     CDS             complement(7176..7571)
                     /gene="msrB"
                     /locus_tag="VF225_09160"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7145460.1"
                     /translation="MMTKVQKTDEQWRRELAPEQYRVLREKGTESPFSGEYDHTFEPG
                     TYRCAGCGAELFSSEAKYDSGCGWPAFYAPAGEEAVDEETDMSHGMVRTEVTCSNCGG
                     HLGHLFPDGPAPTGIRYCINSAALNLEEE"
     gene            7689..7904
                     /locus_tag="VF225_09165"
     CDS             7689..7904
                     /locus_tag="VF225_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145461.1"
                     /translation="MKEAAVLMSFLRHDPVGQRFGVAGLIIAWFALAWTSLALTVAIP
                     LLLVVSEIVRRRREEEAPIVDDLDDLY"
     gene            complement(7912..8316)
                     /locus_tag="VF225_09170"
     CDS             complement(7912..8316)
                     /locus_tag="VF225_09170"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7145462.1"
                     /translation="MKLRRTLLVCTAVSFAAIDLVHKSFASAEFHHPRTQFVALIMAA
                     LIAALVILVPRVPSNTAAIGAGIACGGALGNLISLFAWSQGVPDPLVVEGTMHGLAFN
                     LADLFAIAGDALLLSAVVLHGMRHRARLGESV"
     gene            8374..9030
                     /locus_tag="VF225_09175"
     CDS             8374..9030
                     /locus_tag="VF225_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145463.1"
                     /translation="MGQRRYPECVAREGKPRLRSLEDVVALPRRSPLAAELQRALAAA
                     SRLHGLRSDLSPVPVVATATISEAGAYRFRKRDPIDLRVSRIGGRSALGFLHELGHLL
                     DHQIFYDRKTRSWASAVHQAFAPWRDAAALLEKRVLPGGHSRQRYFQSVHEVWARSYA
                     QTVLLRSEDSALGRQLERLQSEDDAHIWQAEQFAPVAIEVELVFERLGLRQLSLPLAA
                     "
     gene            complement(9027..10526)
                     /gene="gcvPB"
                     /locus_tag="VF225_09180"
     CDS             complement(9027..10526)
                     /gene="gcvPB"
                     /locus_tag="VF225_09180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7145464.1"
                     /translation="MKLIFEKSQSGRRGGELPRYDVPPARVPAELRRERPPRLPELAE
                     PEIVRHFTELSTRNFGIDTGFYPLGSCTMKYNPRVNERLAMLPGFRDLHPYQDEDAIQ
                     GALELMWRLQEALIEVSGLQACSLQPAAGSQGELTGLMLMRAYFADRGEADRRDTIIT
                     ADTAHGTNPASVTMAGYKLAKVETDARGNVDLAHLRELVSERTAGLMLTNPSTLGLFD
                     EHIEEIAEIFHDVGALLYYDGANLNAVCGISRPGDMGFDIVHINLHKTFSQPHGGGGP
                     GGGPIVVRERLEPFLPVPAVVKDGGRFRLDFDRPKSIGKVRGFSGPFGVFVRSYAYIR
                     SYGPRLREMSEVAVLNANYLLARLKETYDLPYDRLAMHEFVLSARSLKREHGISALDV
                     AKRLMDYGFHPPTIYFPLVVPEALMIEPTETEAKETLDEFAEAMLAIAQEAAEEPEVL
                     RDAPHGRPVKRLDEVLAAKRAIVKYGFEEHPDLSNEQAEPRQLEAQKGA"
     gene            complement(10523..11854)
                     /gene="gcvPA"
                     /locus_tag="VF225_09185"
     CDS             complement(10523..11854)
                     /gene="gcvPA"
                     /locus_tag="VF225_09185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX7145465.1"
                     /translation="MSYLSLTERDREEMLAAIGVSSVEELFRDIPAGVRLGRELDLEP
                     SLSEQELSRHLEELAARNIDTSKELSFLGAGIYDHYVPAVVDTVLQRGEFLTAYTPYQ
                     PEMSQGVLQAIFEYQTVICELTGMDVSNASGYDGTTVAADACYVAKHATGRTKVVVTE
                     ATNPQVRQVVKTYAPGFGLEIVEVPHRGGVTDPDELRRAATDAACVIFQQPNFFGCLE
                     PAPDLAAAANDAEAIAVAHVDPISLGVLEAPGAYGCGMAIGEGQGTGNYQSFAGPHYG
                     FLAARKEFIRRMPGRIVGETLDAAGERGYVLTLQTREQHIRREKATSNITTNQTLLAL
                     AGLVHLSWLGPQGLREVGETCMSLAAYAQRRLTAAGLELVFPEQTTFKEFAVRTGRSA
                     TDAIRTARERGVHPGYALGRDYQGLDDAVLVAVTERRTPADIDRLVEALTP"
     gene            complement(11923..12318)
                     /gene="gcvH"
                     /locus_tag="VF225_09190"
     CDS             complement(11923..12318)
                     /gene="gcvH"
                     /locus_tag="VF225_09190"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7145466.1"
                     /translation="MAASETYPDDLKYHAEHDWARVEGDEAVLGITWFAQDALGELVH
                     YEPPNEGDTIAKDSAYGEVESVKAVSDVIAPLSGEVLEVNAKVIEEPETVNEDPYGDG
                     WLIRIRMTNPSEADSLLGLDAYKQVVAEA"
     gene            complement(12325..>12575)
                     /locus_tag="VF225_09195"
     CDS             complement(12325..>12575)
                     /locus_tag="VF225_09195"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX7145467.1"
                     /translation="RGPERKLVSFVMEEKAVPRQGMAIEGRGEVTSGTHSPMLDRGIG
                     MGYVRADSATPDTELAIDVRGKSRRARVVKRPIYKREE"
BASE COUNT         2111 a         4247 c         4086 g         2131 t
ORIGIN      
        1 gggtgcacgg tggtcgacac gagcacgccg cgctccttca tgcgctcgat cgcaccgccg
       61 cggtcgggac cgacgtcgat ctgaacgaag tttgtctcca cctggtcgag gtcgacaccg
      121 acgcccgctt ccgcgagacc ttcggcaagg cgccgcgcac gggcgtggtc gtcggcgatg
      181 cgatccacgt ggtggtcgag cgcgtagagt gccgccgccg cgacgatccc cgcctggcgc
      241 atcgcgcctc cgaagtagtg cttgccgcga cgcgcctgcg ccatcagttc cgcggagccc
      301 gcgatcagcg cgccgagcgg acagccgagc cccttcgaga agcagagggt gacggtgtcg
      361 aagccgcgcc cgatcctcgc cgaatcgacg ccggtcgcga ctgcggcgtt gacgagccgc
      421 gcgccgtcga ggtgtacggc gaggtcgtgc tcgcggcagc ttgcgacgat cgcatcgacc
      481 tcgtcgagcg gccaggcgcg gcctccggca ctgttgtgcg tgttctcgat cgtgaccacg
      541 cgggtcggcg cgacgtgcac gctcgtccgg tcgcggatct ctgagacgac ctggcccggc
      601 gaaaaccggc ccttcgtccc ctcgagaccg atggtcagca gacccgaatg ggccgccggc
      661 ccgcccagct cggagagcat gatgtgggcg tgccgctcga cgagcagggc atcgcccggg
      721 cggccgagga tgttcagcgc gatctgattg gccatcgtcg cggtcggaac gaagaccgcc
      781 tcctcctgcc cgaggaactc ggcgcctcgg cgctcgagct cgttgacggt cgggtcctca
      841 cgcttctggt cgtcgccgac tgcggcgttc gcgatcgccg ctcgcatcgc cggcgtcggc
      901 tgggtcaagg tgtcggagcg gagatcgatc accggcctat tttgcagttt tgacactcac
      961 ccgagcgggc gatcatcagc gacccgtccg gagccgatga aagagccgga aggtgtgtag
     1021 atgaatccct ctgacaagcg ctacgttggc ggcggcctcc ggaccgcctc gggatcggcc
     1081 gtcccgcttc gactcgacca cggctacttc gagcacgagg tccgccccat gctcggctcg
     1141 atcctcgtgc agaagggctg gatctcgcac gagcggctcg acgccgcgct cgcggaggtc
     1201 aaggtcaccg gcatgcgcct cggcgaaacg ctccttgccc gaggctggct cttcgagccg
     1261 gaactcgcat ccgcgctcgc cgaacaggcc gggttgcgct acgtcgatct ggtccgccat
     1321 cccctcgacc cgtcggcggc acggcttctc cctctcgagg ttgctcgtcg gatgttcgcc
     1381 gtaccggtgc gtttcctgga atcgggacgc atcgaagtcg ccgtcggcga tcctgcagac
     1441 gtcgacgtcg acctgctcga gcgcgtgctg gggcggggag tcgacctggt ggtcggcgag
     1501 cgctcggcca tccaggacgc gtggcgttac gcgccttcct gcgctaagta agcgcgttca
     1561 tcccgacctg gggacgctcg ggacaaagct accctgggcg taatggatcg tacgttgttg
     1621 caacagcgtc tcagcgagcg cggcgaggct gcataccgcg cggggcaggt ctgggaatgg
     1681 gccgcccgtg gtgccgcggg ctacgacgag atgacgaaca tccccggctc gctgcgtgcg
     1741 gaactggaag atgaggtgcc gttctcgacc ctcgagctcg tccacgaagc aaaggcaagc
     1801 gacggaacgg tcaaggcgtt gtttcgcacg ggcgaggacc atccagtcga ggcggtgctg
     1861 atgcgctacc gcgacggccg gcgcacggtg tgcctgtcgt cgcagtccgg gtgtccgctg
     1921 acgtgcgcct tctgcgcgac tgggcagatg cgcttcgggc ggaatctgac cgcatccgag
     1981 atcctcgacc aggccttgta tttccgccgc atcgagccga tcgaccacgc ggtcttcatg
     2041 ggcatgggcg agccgatgct gaacctcgat tcggtgctcg aggccgcgcg ggctttgccg
     2101 gacctcggga tcacctggcg tcgcacgacg atctccacgg tcggttggct gcccgggctt
     2161 cgccggttcg tcgacgaggt cgaggagccg atcaggctgg cgttctctct gcacgcgccg
     2221 accgacgagc ttcgcagcga gctgatgccc gtgaacgacc gctacccgct cgccgccgtg
     2281 atcgcggagt gcactcgcta cttcgcagtg caccggcgga aggtattcgt cgaatacgtg
     2341 atgctgcgcg gcgtcaacga ccgcgtcgag caggcgcgcg cactcaccga tctcctcgac
     2401 ccgaaggtct tcaaggtgaa cctgatcccc tacaacccga ccgggatgta cgacgggtcc
     2461 acgtcgaagg cgatcgcagc cttcaaggcg gcgctcgatc gcgcacggtt gccggcgacc
     2521 gtcaggctca cgcgcgggcg tgacatccag gccgcttgcg gccagctggc cgtggtggaa
     2581 gccaagggac agctctgaat ggcgtggccc tgggaggtcg tcgagcgcga tcacgagatc
     2641 cagaacccga cgagccccga gaagatccgg gttctcggtg agtacctgcg cctcacgagt
     2701 gagagccgcg tcctcgacat cgcctgcggc aaaggcggcc ccgctctgat cctcgcggcc
     2761 acctacggct gccgagtcca cggcgttgag atccggccgg cctttgccga ggtagcccga
     2821 gcgcgggccg ctgcgaacgg actcgagtcg ctgatcgagg tgcaaacggc tgacgcgtct
     2881 gagctcgagt tcgaaccgga atcctgcgac gcggctctct gtatcggagc cagcttcgtc
     2941 tggggaacga tcgccgatgc ggccgcggcg ctcctccctt tcgtgcgagc aggaggcttc
     3001 gtcgcgatcg gggagccctt ctggaggcaa tggccactac ccgacgggat cgaggaagag
     3061 gacttcgtcg atctccacgc aaccgtcgag cggttccagc gagccggtct cgcatccacc
     3121 ggcgtgatcg ccgcctccga ggacgactgg gacaactacg agagcctgca ctggagagca
     3181 accgaggaat ggctggccga acatcccgat caccccgacg cggaggacat acgcacccag
     3241 cacagccgct atcgcagcga ctacttccgt ttcaagcgag cgttgctcgg ttgggcgatc
     3301 ttcgtgggcc gcaaggtctg agccggccgc tcgtggctta cgcggcggct agattccggc
     3361 ggtgcacgac gaggacggct aagccggccg tctaggccgt gctcgactac gtcagcgcct
     3421 gacggcgcgg tcgtagttga gccgcagcag ctcgatcacg tcgcggacgt cgccgtcgtc
     3481 gacgatcagc cgcgcagccg gcccttccgc atccggaaag accggatgcg gctcgattcg
     3541 cccctgctcc ttcaactcgc gccagacctg cttcgggaac ccaaagtgcg cggctcggtc
     3601 gccgtgcagg tgaccgatct cacggccgcc gaccttgaaa gcgaactcac cgcgcctccc
     3661 gggtccagcg gttacgccgg gccacgatgt gacctcctcc gtaatccgcc cactcgccga
     3721 ctggatcttg ttcattcccc gctccgcgcg aacctcgcaa gcttgctgcg cacgcgctcg
     3781 atctctgcgc catacatgag gagctcgtca tcgtccgcac cgcgtcgttc gcggacactc
     3841 cggaccagta ccaggccctt caaggtcagg actaggtcgt cgagcttgcg gaagccgtcg
     3901 ctgaatccct cttcgccggc catcacgcgg cctcttccag tgcaatcggt tcgccttcaa
     3961 cagccggctc gtccgcgcct aaggccggag ccgacttgac gagcgtggcg gcaatcaccg
     4021 cgccgatgac cagcagccct gtgagcacgt agagaccggt ctggaagccg tggtcgagcg
     4081 cgacgctgct ggacggggta agtgcggagt ggacgtgggc gtagttactt gtcgacgtcg
     4141 cggcgatcgc gctcaccgcg gcgaggccga tcgcgccccc gacctggcga ctcgtattga
     4201 tcagccccga ggccacaccg gcatcggagc gttcgacgcc ggtgagactt gcgatcgtga
     4261 tggggacgaa tgaaagcccc atcccggcgc cgccaagaac gaaccacggg aagagatccc
     4321 agaaatagtg cccgtcgacc ggaaggcgcg tgagcgccgc aaccgacacg gcggaagcga
     4381 ccagcccggc ggtgagcgtc ggcttcacgc caaaacgtcc cacgacgacc tgggcgacgt
     4441 tcgatgccac gacgaccgcc gacgcgaagg cgacgaacgc agcaccgctc tgcattgccg
     4501 agtaatgcaa gacgtcctgt aggtagagcg tcaagaggaa gaactcggag aacgcgaccg
     4561 agccgacgat cgccatcgcc gcgttcgcgg ccgtgagcga gcggacgcgg aaaatccgta
     4621 ggggcaggag cggtgacggc gagcgcaact cgatcgcaat gaacgcaagc accaggcctg
     4681 ccgctccggc gaggagtgcg attgtcgtac ccgagcccca gccgtcggtc gttgcgcgcg
     4741 tcgtcgcgta gacgagcagc atgagaccgc cggtgatgga tgccgcgcct gggacatcga
     4801 agtgtcggtt cgccaaccca gcacggctct cccgcagcag cagaggcgtg agcacgatcg
     4861 cggcgacgcc gacgggaatg ttgatgaaga agacccatga ccagctcaga taggacgtga
     4921 gcacgccgcc gagcaggacg ccggccgcgg cgccgctccc ggaggcggca ccgtagatgc
     4981 cgagcgcgag attgcgttcc cggccctcgg cgaacgtcgt catcagcagc gagagtgcag
     5041 ccggagcgag cagcgctccc ccgagccctt gcagcgcacg gaacgcgatc agcgagctct
     5101 ccgaccaggc gaggccgcag agcagggagc ttgtggtgaa cagggcgagc cccgcgacga
     5161 agagtctccg gcggccgagc aggtcggcga gcctcccgcc gaggagcaac gctccgccga
     5221 acaggatggc gtaggccgag atcacccact ggaggttcgc ctgggaaaaa ttgagatccg
     5281 tcttgatcga cggcagggcc acgttcacga tggcgacgtc gagaacgacc atgaactgtg
     5341 cagtgacaat gagagccagc gcactccagc gccgctcgct cttcgtgaga atatccacta
     5401 attggcctcc aaaatcgttg atatgctcaa tgttatagca cgacaacgct tgtaggcgct
     5461 actctgtttc acatggccct cacaacccct gaaacacaac cgcgcaccgt gccgcagctg
     5521 cccggggagc tcgttgcatc agccacattc ctgctcaaga aactgggatt cgccgcgaag
     5581 gatcggacga tgaaggccta cgaagggact ggactccacc cgtatcacca cgcgatcctg
     5641 ctcgtgctcg atgtggggtc ggccgagacg cagggctcga tcgccgacgc gctcggctac
     5701 gaccgtggcc agctcgtggg gctgctcgac gagctcgagg agcagggact catcgagcgg
     5761 cgtcgcgatc ccgacgaccg ccgccggcac ctcgtccgtc tcacgtcgga cggcaagcgg
     5821 gcgctgaagc ggctacgcgc gctctcgaaa gagatcgagg acgagttcct gtccccgctc
     5881 ggcgacaagg aacgagcgaa cctgcacgcc cttctgcttc gcctcgccga aaagcacgag
     5941 ccgcgctgtg cgctcagcgt cccgttgccg cccacgcggt agcgacaagg acacgggcgg
     6001 cggcgcgtca gagtcacggc cttgatcggc aaatggttcg agtcatacga cgaggcgtgg
     6061 agctacttcc tcgcgcgcga gacgccgctg gaggacttct ggtcggtact cccgaaacct
     6121 gagggaagca tcctcgccgg ctggctgatc gaacctccgt ctcaggcgaa agacgccgtt
     6181 cacgatctgc ttgcgcgcct cggaaaggtc gaaggtgttg ctgtagttcc ctcgcacttc
     6241 ctgcacatct ggatccacgg cgttgcgcac gaccctaccg ccgaccggat gagcgagctc
     6301 gtcgacgaag ctcgcgaggc ctgggcccgg cgaggctctt gcgagattac gctccggcac
     6361 gcgaattgct tccacgatgc cgcagtcatc gaggctcaca cggacggcgt cccacgcttg
     6421 ctcgcagcgt tgaatcccga cgccgacctc cgtgttgcac tccctcacat gaccgtcgct
     6481 tatctgcggt caccgtcagc tgcttcggtg cgcgacgtcc tcggggactt cagaggccaa
     6541 gagttcgggt cgttccacgc cgacaatgtt cggctctgcc tgatcccctt cgcgcgctcg
     6601 accttcatgc gcccgtggaa ggtcgccgcc acgcacccgc tctcgagtgc gtgacggcat
     6661 tcgcgggctg cgggtctagc ctcagctcgc ggcagtggcg cgctgcagcg cgggcgtgca
     6721 gctcgagcgg ccgcgcttct cgaggtactg ctggtggtag acctcagcct cgtagaaggc
     6781 ctgcgcgggc tcgacctgcg tgacgatcgg cgcgccccag ttccgctgct ctccggcctt
     6841 cgacttcaaa gcggcctcat gctgctcctc gtcgtggaag aagatcgcgg agcggtagtt
     6901 gtcgccgatg tcccagccct gtcgattcag ctgcgtcggg tcgtgctttg cccagaagac
     6961 gtcgagcagc tgctcgtacg agatgcggtc ggggtcgtac gtgacctcga cgacctcagc
     7021 gtgccctgtc gtgtgcgagc agacgtcctc gtacgtcgga ttctcggtcg tgccgcccga
     7081 atacccgacg cgcgttcgcg tcacgccctc gagctgccgg aatgcagcct cgacgcccca
     7141 gaagcagccg gcgccgaacg ttgccttctt ctccactatt cctcctccag gttgagtgct
     7201 gccgaattga tgcagtagcg aatcccggtc ggtgcgggac cgtctgggaa cagatggccg
     7261 aggtggccgc cgcaattcga gcacgtcacc tcggtgcgca ccatcccgtg ggacatgtcg
     7321 gtctcctcgt ctacggcttc ctcgccggca ggggcgtaga acgcgggcca gccgcacccg
     7381 gagtcgtact tcgcctcgga gctgaagagc tcggcgccgc agccggcgca gcgataggtg
     7441 ccgggctcga aggtgtggtc gtactcaccg gagaacggcg actccgtgcc cttctcgcgc
     7501 aggacgcggt actgctcggg agccagctcc ctgcgccact gttcatcggt cttttggact
     7561 ttcgtcatca ttttctccct cattcgggtt cctccctaca gtcttgctac tggcgcaggt
     7621 attacgcgac cgagggatag aggattcccc cgggccggcc gatccctgcc cttgaaggag
     7681 gagccaaaat gaaggaagca gccgtgctca tgagcttcct gcggcacgac cctgtcgggc
     7741 agcgcttcgg cgtggccggc ctcatcatcg cctggttcgc actcgcctgg acgagccttg
     7801 cgctgacggt cgccataccg cttctgctcg tggtgtcgga gatcgtccgt cgtcgtcgcg
     7861 aagaagaagc gccgatcgtg gacgacctcg acgatctcta ctaagacagc cctagacgct
     7921 ttcccccaat ctcgcgcggt gccgcatccc gtgcaggacg accgcgctga gcagcagtgc
     7981 gtcgcctgcg atcgcgaaaa ggtcggcgag gttgaaggca aggccgtgca tcgtcccctc
     8041 gaccaccagc ggatcgggca cgccctgcga ccaggcgaag agcgagatca gatttccgag
     8101 tgcaccgccg cacgcgatcc ccgcgccgat cgcggcggtg ttcgacggga cgcgcggcac
     8161 aaggatgacg agtgccgcga tcaacgcggc catgatcagc gcaacgaact gcgtacgtgg
     8221 atgatgaaac tccgccgacg cgaacgactt gtgcacgaga tcgatcgccg cgaaggaaac
     8281 ggccgtgcaa acgagaagcg tacgacgcag tttcaccctt caaagcatga caggcagctc
     8341 aagtccgtcc gatgcacaac cgattattta ccgatgggac aacgccgcta ccccgaatgt
     8401 gtcgcccgcg agggaaagcc aaggctgcgg tcgttggaag acgtcgtcgc gttaccacgc
     8461 cggagcccgc tggcggctga gttgcaacgc gcgcttgcag cggcgtcgag gctgcacgga
     8521 ctgcgctccg atctttcacc tgtgccggtc gttgcaacgg cgacgatcag cgaggccggc
     8581 gcctaccgtt tccgtaagcg cgatccgatc gacctgcgcg tctcacgcat cggcgggcga
     8641 tccgcgctcg gattcctgca cgagctcggc cacctcctgg accaccagat cttctacgac
     8701 cgcaagacac gctcgtgggc ctcagctgtg catcaggcgt tcgcaccctg gcgcgacgct
     8761 gcggcgctgc tcgagaaacg agtcctgccc ggaggccact cacgccagcg ctacttccag
     8821 tcggtgcacg aggtgtgggc acgctcctat gcgcagactg tgttattgcg gtccgaagac
     8881 agcgcgctcg gccgccagct ggagaggttg cagtccgaag acgacgcaca catctggcag
     8941 gcggaacagt tcgcgccggt ggcgatcgag gtcgagctcg tcttcgagcg gctcgggctc
     9001 aggcagctga gcctgccgct cgcagcctag gcgccttttt gcgcttcgag ctggcggggc
     9061 tccgcctgct cgttactcag gtccggatgt tcctcgaagc catacttcac gatcgcgcgc
     9121 ttcgcggcga gcacctcgtc cagccgtttc accgggcgtc cgtgcggcgc gtcgcgaagc
     9181 acctccggct cctccgctgc ctcttgcgca atcgccagca ttgcctccgc gaactcgtcg
     9241 agcgtctcct tggcctcggt ctccgtcggc tcgatcatca acgcctccgg aacgacgagc
     9301 gggaagtaga tcgtcggcgg atggaagccg taatccatca gacgcttcgc gacgtcgagt
     9361 gccgagatgc cgtgctcgcg cttcaacgaa cgggcgctca gcacgaactc gtgcatcgcg
     9421 agacggtcgt acgggagatc gtacgtctcc ttgagtcggg cgaggagata gttggcgttg
     9481 aggaccgcga cctcggacat ctcgcggagg cgcggtccgt atgagcggat gtacgcgtag
     9541 gagcggacga acacgccgaa cggtccactg aagccgcgca ccttgccgat cgacttcggg
     9601 cggtcgaagt cgagacggaa ccgcccaccg tccttcacga ccgccgggac cggcaggaac
     9661 ggctcgagcc tctcgcgcac cacgatcggg ccaccgcccg gacctccgcc cccgtgcggc
     9721 tgcgagaacg tcttgtgcaa gttgatgtgc acgatgtcaa agcccatgtc gcccggacgc
     9781 gagatgccgc agaccgcatt caggttggca ccgtcgtaat agaggagagc gccgacgtcg
     9841 tggaagatct ctgcgatctc ctcgatgtgc tcgtcgaaga ggcccagcgt ggaggggttc
     9901 gtcagcatca gcccggccgt ccgctcactc accagctcgc ggagatgcgc gaggtcgacg
     9961 ttgccgcggg catcggtctc gaccttcgcg agcttgtagc cggccatagt gacgctcgcg
    10021 gggttggttc catgcgccgt atcggcggtg atgatcgtgt cgcgccgatc ggcctcgccg
    10081 cggtctgcga agtaagcgcg catgagcatc aggcccgtca gctccccctg cgatcccgcc
    10141 gccggctgca acgagcaagc ctgcagtccg gatacctcga tcagtgcctc ctggagccgc
    10201 cacatgagct cgagcgctcc ctggatcgca tcctcgtcct ggtacgggtg gaggtcgcgg
    10261 aaacccggca gcatcgccag ccgctcgttg acgcgcgggt tgtacttcat cgtgcaagag
    10321 ccgagcggat aaaagcccgt atcgatgccg aagttgcgcg tcgagagctc tgtgaagtgc
    10381 ctgacgatct cgggctcggc gagctcgggc aatcgcggcg gcctctcccg tcgtagctca
    10441 gccggcaccc tcgcgggcgg gacgtcatag cgcggcagct ctccgccgcg ccggccggat
    10501 tgcgacttct cgaagatgag cttcacggcg tcagcgcttc gacgagccgg tcgatgtcgg
    10561 cgggtgtgcg acgctccgtg acggcgacga gcactgcgtc gtcgagcccc tggtagtcgc
    10621 ggccgagcgc atagcccgga tggacaccgc gctcgcgggc agttcgaatt gcgtccgtcg
    10681 cgctccgtcc ggtgcggacg gcaaactcct tgaatgttgt ctgttcagga aacacaagtt
    10741 cgagtccggc ggccgtcagc ctgcgctgcg cgtaggcggc gaggctcatg caggtctccc
    10801 ccacctcacg cagtccctgc ggccccagcc acgaaaggtg cacgagcccc gcgagggcca
    10861 gcagcgtctg gttcgtcgtg atgttcgagg tcgccttctc gcgccggatg tgctgctcgc
    10921 gggtctgcag ggtcaggaca tagccgcgct cacccgcggc gtccagcgtc tctccgacga
    10981 tccggcccgg catgcggcgg atgaactcct ttcgcgcagc aaggaagccg taatgcggcc
    11041 cagcaaagga ctggtagtta cccgtgccct ggccttcgcc gatcgccatg ccgcagccgt
    11101 aggcacccgg cgcttcgagc acgccgagag agatcggatc gacgtgcgca acggcgatcg
    11161 cctccgcgtc gttggcggcg gcggccaggt cgggcgccgg ctcgagacag ccgaagaagt
    11221 tcggctgctg gaagatcacg caggccgcgt ccgtcgccgc ccggcgtagc tcgtccgggt
    11281 ccgtgacgcc cccgcgatga ggcacctcga cgatctcgag cccgaagccc ggcgcgtacg
    11341 tcttgactac ctgtcgcacc tgcggattcg tcgcttcagt cacgacgacc ttcgtccgtc
    11401 ccgtcgcgtg cttcgccacg tagcaggcgt ctgctgcaac ggtcgtcccg tcgtagcccg
    11461 acgcgttcga gacgtccatc ccggtcaatt cgcagatcac ggtctggtac tcgaagatcg
    11521 cctggagcac cccctggctc atctccggct ggtacggcgt gtatgcggtc agaaactcgc
    11581 cacgctggag cacggtgtcg acgacggccg ggacatagtg gtcgtagatg ccggcgccga
    11641 ggaacgagag ctccttgctc gtgtcgatgt tccgcgctgc aagctcttcg aggtgccgag
    11701 agagctcctg ctcggagagc gacggctcga ggtcgagctc gcgcccgagg cgcacgcccg
    11761 cggggatgtc gcggaagagc tcctccaccg aggagacccc gatcgccgcg agcatctcct
    11821 cgcggtctcg ctccgtcaat gacaaataac tcatcgttct cccccgagcg aagctacggg
    11881 actaaccctc atcgcggaaa atgcgcagca tctttcgcga cattaagctt cggcgacgac
    11941 ttgcttgtag gcgtcgaggc cgagcagcga gtcggcctcc gagggattcg tcatgcggat
    12001 gcggatcagc cagccgtcgc cgtacgggtc ctcgttgacg gtctccggct cctcgatcac
    12061 cttcgcgttg acctcgagca cctcaccgga gagaggcgcg atcacgtccg agacggcctt
    12121 gaccgattcc acctcaccgt acgccgaatc cttcgcgatc gtgtcgccct cgttgggcgg
    12181 ttcgtagtgg acgagctcgc cgagcgcgtc ctgcgcgaac cacgtgatcc cgagcaccgc
    12241 ttcatcgccc tcgacgcgcg cccaatcgtg ttccgcgtgg tacttcagat cgtcgggata
    12301 ggtctcgctc gcggccactt ggtgctactc ctctcgcttg tagatcggtc tcttgacgac
    12361 acgtgcccgt cgcgacttcc cgcggacgtc gatcgccagc tcagtgtccg gagtcgcaga
    12421 gtctgcacgg acgtagccca tcccgatacc gcggtcgagc atcggcgagt gcgtccccga
    12481 agtgacctcg ccccggccct cgatcgccat gccttggcgc ggcaccgcct tctcctccat
    12541 gacgaacgag acgagtttgc gctcggggcc acgag
//
LOCUS       DASVEU010000214        21775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_731493,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000214 DASVEU010000000
VERSION     DASVEU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 21775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21775
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731493"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF225_09200"
     CDS             complement(<1..223)
                     /locus_tag="VF225_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003835684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145468.1"
                     /translation="MNWSLFFDLTVSGLTLGSLYALIALGYSLVYGILKLLNFAHGDV
                     YMIGAFLGWYALHALGGPMNPTFTGAVVLL"
     gene            complement(299..1501)
                     /locus_tag="VF225_09205"
     CDS             complement(299..1501)
                     /locus_tag="VF225_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7145469.1"
                     /translation="MRRTRATAVAALLALGAALAVVAGAYATTERAAAPTAQTGSDSA
                     LIKCGKTRTIGFMAPVTGPAASIGIQQVHWAQYYVSTYNKTHKKTKMKLQTEDTQLGA
                     PTGTAEALKGAQALAGSPNVLGVVGPAGSNEIVGVTKSLMDGGLAWVSGSSTRTSLTT
                     DGTRTGYMFRTVPPDAIQGPTAANYIIKVLKYTRVYIIDDQETYSTGLADEVQSKLKA
                     AGVTVGRDGVSQQQSDFSSLIAKIARDTQLVYIPWQLTPKGIAFGNQMKAAGKGSIKL
                     MGADGLFDSSFAAVGSHVYDTNFPLNPKSKIIAGFKKGHGGDGEYFGAPTYAATQVVA
                     TAIDRACKKGTATRASVRAAIRATNIPAAQSLIGVALRFNRNGDLSTAKKFGLYKSNG
                     TVFVPIPG"
     gene            complement(1559..2287)
                     /locus_tag="VF225_09210"
     CDS             complement(1559..2287)
                     /locus_tag="VF225_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145470.1"
                     /translation="MAAVSSFVIDENDLEPIQDDGDTASVYIAFDAANGCERLEQRVI
                     RFGPGRSVERTLAGQQEVLYIVAGRGQLHVDGNTHELEPNMGVYLAPGETYAVENPGP
                     HDLHVLSVIAPEDRAAASGERKVTVRFDDQPELEASVERSFRYLVNEDAGCFDVTQFL
                     GIVQPSKAPFHSHTYDEVGYIVAGEGIAHVGGAEFPLKTGSCFHLPPDEVHCIENTGS
                     APLRILGVFHPSGSPANRTYVDNK"
     gene            complement(2297..3103)
                     /locus_tag="VF225_09215"
     CDS             complement(2297..3103)
                     /locus_tag="VF225_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX7145471.1"
                     /translation="MPAVTRISVAPVKGLGLVHPESVSLERHGVRENRRFHIVDADGR
                     RFNQLRNGALVQILPEYDADSERLTLRFPDGSVADGEVSLGTEVTTDFYGRPVVGSYV
                     QGPWSGALSEWAGVQLRLVQSPPGLAVDRGRGHVSLISAASLAELGQNGDHGRPVDGR
                     RFRMLFELEGCAPHEEDGWVKRHLRIGEATIRIRGDVGRCAITTQNPDTGEPDFDTLR
                     TISSYRGFTENEAGKRHIPFGVYGEVTERGRVTVGDGVEVLALSLLDQTT"
     gene            3209..3763
                     /locus_tag="VF225_09220"
     CDS             3209..3763
                     /locus_tag="VF225_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7145472.1"
                     /translation="MPRPPRNAYPGGFFHVGTRGNSRCRVFLDATDYKVFLMMLDRSE
                     RKYNWRVYSWCLMTTHYHVVVATPDGALSQGICELNGSFARWSNLRHEREDHLFGKRF
                     GSKEILTNEQLKEACRYVVLNPVRAGMCEDPADWRWSSYRASAGFDEAPPYLALGELL
                     ALFDVDPGKAISLYRDFIDQGLAS"
     gene            complement(3806..4546)
                     /locus_tag="VF225_09225"
     CDS             complement(3806..4546)
                     /locus_tag="VF225_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7145473.1"
                     /translation="MVRIAAAGDVHASEATRSRIAEAFAAVEREADLVLLAGDLTTQG
                     EPEQAEALAEATRSLRVPVVAVLGNHDLHSGHGEQISSILVEAGVRMLDREAIVLELV
                     GREIGVAGTKGFVGGFSGSQLPDFGEPLLRRVYAETTEEVDAIRRGLQEIAHCPIRIV
                     LLHYAPTSDTLHGEPEGIWTYLGSDRLATPIAEYRPDVVLHGHAHSGSFEGAIGGVPV
                     YNVAVHVTGRDFYVFELEGHDVEVETRA"
     gene            4590..5153
                     /locus_tag="VF225_09230"
     CDS             4590..5153
                     /locus_tag="VF225_09230"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX7145474.1"
                     /translation="MNTDQEREFERLLAAMKKAGGALKGAGVPFVLGGGLACWARGAP
                     KTEHDVDFLVKPEDAERARRTLADAGMRTETPPEGWLLKAYDDGVLIDLIFDPHDGPI
                     DDGTFERAEELEVHAMRMKVAALEDVLVQKLLALKEQEPDFSSVLELARALREQVDWD
                     EVRERTRESPFAKAYFTLLDELEIVPA"
     gene            complement(5160..5975)
                     /locus_tag="VF225_09235"
     CDS             complement(5160..5975)
                     /locus_tag="VF225_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX7145475.1"
                     /translation="MKVFIVSDMEGVAGIVKWQQTDGTKEKRSYHEGRELYTEEINAA
                     VRGARTGGATQILVMDCHGAGEEWTFNSLIPDKLDRGCEYVVQNDWTEYTEFLEQGCD
                     AALFVGMHAKAGTPDGVLSHTVSGQAWRELRFNGVPVGETGINAALCGQWGCPVLLVT
                     GDEAVCREGAELLGSGLTTVAVKKGLGRFSARQVPPLRAREMIEDGVRRALRDLSAVK
                     PYDPGRPCEITIEFMTPDRVVEYRNRRGVEQVNDLTLSSKADDWWSAWSQFYF"
     gene            complement(5972..6451)
                     /locus_tag="VF225_09240"
     CDS             complement(5972..6451)
                     /locus_tag="VF225_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7145476.1"
                     /translation="MREQMTWEDLGTAVDDLAAQIRADGFGPDAVLALARGGLACAGA
                     LAYALGVKNMATLNVEFYTGVDERLDEPLLLPPVPDLALLRAERLLVVDDVADTGRTL
                     ALASEFCLQHGPEVRTAVLYEKPQSVVRCDFVWRRTDRWINFPWSTPAPVAAGQAGS"
     gene            6471..7232
                     /locus_tag="VF225_09245"
     CDS             6471..7232
                     /locus_tag="VF225_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7145477.1"
                     /translation="METGLAGKRVLVTGASGGIGSACARAFAAAGADVVVHYHRGKER
                     AEQLAGELGGGAAVVGADLTREDEVDSMFATAGGLDVCAAVAGVWPSEDLPVWELPLE
                     RWRATLDANLTATFLTARGFLGQLEGDGALILVGSTAGIVGEAGHADYAAAKSAILGG
                     LLLSLKNEVVRKSPQARVNAVAPGWTESAMTRGHVSKEQVRRISRTMALRKVAQPEDV
                     ANQVVVLASPVLSGHVTGQVVTVAGGMEGRVIHET"
     regulatory      7614..7718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7712..9568
                     /locus_tag="VF225_09250"
     CDS             7712..9568
                     /locus_tag="VF225_09250"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX7145478.1"
                     /translation="MCRASSEGDEVPAQSQTRNRFLERLQSGPVIVGDGGMGALLSAA
                     VPRLRSPEEANLRAPDAVVSLHVSFINAGAELIETNTFGANRRKLSHHFLEDDLEPIN
                     SAGVKLARDAREMTGRDVFIGGSIGPLGEPATSRVRRELFTEQAAVLDGRGVDLFMVE
                     TFYDLEEAVDAIEAVRSASSLPIVALLTFDESAETLAGVTAAEAAARLTELDVAAIGA
                     NHGAGLLAALAALEQMGKNGKPLAALPNVGLASLTAGRVIYPHATPEYFGEFAAHARD
                     LGAKVIGGCCGTTPAEIAAIRSAVEDERKPRAPLVFDTPELVVALGEEQKETGLSRAL
                     AAGEWVVSVQLDPPLGGNSAGLLEVASTLKESGRVGWVDVNDNATARAGMSSLMVSAT
                     IERRAEIETIPHLTTRDWSVMGLESMLLGAQAEGVRNVLAVTGDPPEVGDYPGARGVY
                     EIDSIGLTQLMTNLNRGEDFNGRPIDAPTSFFPGVAVNPTPDDLDVELDRFEKKLEAG
                     AKFAMTQIVFDADPLDRFVERLGGKWPIPVLVGVFPLTSHRLALRLHNEVPGILVPQA
                     LQDELERAGSDAADVGFAHARELIAASRERAAGVYLVAPFRRPLRVLELLVD"
     gene            complement(9565..10119)
                     /locus_tag="VF225_09255"
     CDS             complement(9565..10119)
                     /locus_tag="VF225_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7145479.1"
                     /translation="MGKLIYSAIASLDGYVADEAGKFDWAEPDEEVHRLVNDLERPVG
                     TYLYGRRMYEVMAYWETAHALPDQPPFVRDFAEIWQAADKIVYSKTLETAATARTRIE
                     RDFDPETVRQLKASAERDITVGGPDLAAQAIRAGLVDELQLFVAPAVAGGGKRSLPDD
                     VRLKLELLDERRFGSGVVYLRYRI"
     gene            complement(10132..11265)
                     /locus_tag="VF225_09260"
     CDS             complement(10132..11265)
                     /locus_tag="VF225_09260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX7145480.1"
                     /translation="MTKVDLIVLGFVVLTAFIGWKKGLIASALSLAGVVLGAWLGSRL
                     APQLLQDGTHSPYTPLAALAGAAVGAILLETLGTLAGSALRSKVRRPKLSTFDATGGL
                     VLGALAGLAVVWVLGAVALLLPGQTDLRRSAQGSALLQRLNKFIPPTEVLNALARIDP
                     FPAITGPSATVAPPDPRLAHAVGVRRAAPSVVRVLGMACGVGVEGTGWVARPNLIVTA
                     AHVVAGQNDTVVELQSGGRLPADVVAFDRRNDVAVLRVQELGLRALPLVSARSGAPVA
                     VLGFPENGPFTATPARIGHTSTVLAEDAYGAGPVTRRITSLRGRVRHGDSGAPAVDAH
                     GAVQTTIFAARLRSTGGFGVPTDVVRADLDAAGQPVSTGDCAP"
     gene            11328..11401
                     /locus_tag="VF225_09265"
     tRNA            11328..11401
                     /locus_tag="VF225_09265"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11362..11364,aa:Asp,seq:gtc)
     gene            complement(11790..13151)
                     /locus_tag="VF225_09270"
     CDS             complement(11790..13151)
                     /locus_tag="VF225_09270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX7145481.1"
                     /translation="MTSLSTRILGLVVGVAVVAAVGAATSRNAQAVLPPLPNTVQQWN
                     KIAEDTVLASGAFQGEGEIYMGYESLAVYDAVVAIQGGFEPYGPAISAPTGASLDCAV
                     VEAAYRTLRHYFSTFPALVASLDGYYSEALSPSNLNGCTGDAGKGTSVGLAAANSIIA
                     LRTGDGRMTPVGTTSSFETKTPGPGVWRLTPPAFLVPQTPWLGSVQPFLLKSPGQFRP
                     EPPIPLSSTEWVRQFNEVKAFGGVTSTVRTSDETATARFYTANVIRQFNGAARDLATA
                     RGLGELETARLVAMVNTVSADALMSVLNAKYRFLFWRPVTAIDPTAVTADGFGPVPGF
                     DDGNPATIEEPGWRPLVTTPNHPEYPAAHGTNTSAMAEVFSEFLGTDDIDLDIHGFDA
                     AGLPGNLDAVHHFDTADQLREEVINARLWGGIHYRRSSEVGVHLGLKVARYGLNHAFR
                     PVS"
     gene            13688..14065
                     /locus_tag="VF225_09275"
     CDS             13688..14065
                     /locus_tag="VF225_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145482.1"
                     /translation="MGAAPRYARPFVAILVGMVVVCALAPLNLWPFSNWELFSRLRGP
                     VESYWEAVVEDHGLVVADYTIGPHTACVPLLRSATARFGPATDVRIYHVERLLSDRLG
                     SQAAPPYRTLVATCTAKGVRAQS"
     gene            14052..14924
                     /locus_tag="VF225_09280"
     CDS             14052..14924
                     /locus_tag="VF225_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145483.1"
                     /translation="MHRVERWIFAPEDARRLAGVRIGICLLLAYRLSAHDYDALAARH
                     VRFLPHFYMDVFAHMPSPDLASALQTLGIAAALAAGLGLALRITLPVALVCGLILNGM
                     LNSTGRVMVRDALLMLCLAVIIAAGRAAGDVWAVRAPNQMAFGERYGWPIRSAMIVIA
                     LAYFFASVQKWRYSGFGWVTSDNLRWILYAQPHPNGFALFIADRPLLAHLFAAGALLL
                     ETSFPLVLFVPRLRWLLIPAVLTMHVAIRIALGLDYSAQALTVLIVFVNWPVVVDWTR
                     RTLAPTPAPREIAR"
     gene            14921..15313
                     /locus_tag="VF225_09285"
     CDS             14921..15313
                     /locus_tag="VF225_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX7145484.1"
                     /translation="MSRTAQSVLLYDEDCGFCRWSLDKILAWDRGKRVRSVAIQSEEG
                     SRLLAPIAPDARLDSWHLVRAGGRLFSAGAAAGPLARLLPGGRPLAALFAAFPGVTEL
                     AYGFAARHRFLLARLLRVDANCQVRHRA"
     gene            15306..16856
                     /locus_tag="VF225_09290"
     CDS             15306..16856
                     /locus_tag="VF225_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145485.1"
                     /translation="MPEQTSRRAKIALATLVAAYLAMAVVAGAPNSPLTVLLPNGATS
                     PTWARLLANAASLADVGRRGLTVVAWVLLVVAVAAFAVVVLEAWSRRIRLAAVLTAAS
                     CSLLIVVAGPLLLSRDVYTYAAYGRIEALYGGNPYFTTLSAFPHDRFVAVTSVQWLHT
                     RSHYGPLFTLASAGLARAWAGSVDGTILAFKVLAGVSLAAATGLVALTARKIRPERAA
                     FAVALVGLNPVVVLHTVGGGHVDAPIAALLAAACACVVTRPRGRSARAFAITVLITLA
                     CLIKTVMLPVLLLWFAWLARPRGVRTLVLHLLVIAALIVAAVGAFFSVSHPTAPFASL
                     GGIESWASPSHFVARDAQTFVALVAGTQAGHDASVVAEAAFPLLFAVLLWRLVRRIRE
                     AGARAPIAEWGIALFLIALSLPYLLPWYAAWFVPFLAFLDDAVLTFAGVFACVVLAFT
                     LVPADPFHGLTTPAVMDGVHYGAAPLLLLVLVVIAVRLRPPAWGGAERHRRAVRVLAA
                     LFASGSRG"
     gene            17032..18240
                     /locus_tag="VF225_09295"
     CDS             17032..18240
                     /locus_tag="VF225_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145486.1"
                     /translation="MNHNASMPRKEFLTKGAAVLGAAVAAPLVGVAGAQAAAERALAG
                     VGRGELGLVGLDHVGITVPNINQAIEWFEDVLGGVAPLTFGPLPAGPFVASVVDVAET
                     TSIDQITMLRIGHSASIELFQYTAPDGQRHDMPKNSDWSGHHVAFYVTDIDTAVEYMV
                     GKGVRKMPAGPFALTQGPAAGQTIQYFQTPWGGYIEFISYPDGMAYQVPSVKPLWSPK
                     LNGADSVVTNVPGLLGIDHVGFTVPNVDVAASWLEQKLGFSNPLTFGPFSDPTGDFMT
                     QLVDVHPRAVVQQIRMLRGGNGPNIELFQYTSPDQDQTFRRNSDWGGHHIAFYVRHID
                     KGVEYLQTNAGTKLFGPVTLTDGPAAGQSINYFHDPFGTDVELISYPHGMAYEETAAI
                     PLWDPLDNHP"
     gene            18514..19032
                     /locus_tag="VF225_09300"
     CDS             18514..19032
                     /locus_tag="VF225_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX7145487.1"
                     /translation="MPTAFRSPLLTFVFVAIVFGCASAFPHEDAAAAVAPQTLTRHDP
                     IPAVPKPTVGERAAAIAVRAVGVPYRWGGSSPAGGFDCSGLVDWAYGRLGIELPHSSY
                     ALYDQGRRVARARMKPGDLLFFSGLGHVGIYIGLGRMVHAPHSGTRVQVVKLGRSSYG
                     ARLVGVRRVIHT"
     gene            19160..20239
                     /locus_tag="VF225_09305"
     CDS             19160..20239
                     /locus_tag="VF225_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7145488.1"
                     /translation="MSRVLLLAFVAALALFPTAAGAGASGVVVSQIYGGGGNAGATFR
                     NDYVELFNAGSGAVDLSGWTVQYATAAGTTWQTTALSGTIAPGRYYLVQLASNADVGA
                     VLPSPDATGTSNLGGTSGKIALVRGAAAITCGASAGSCSADPLVEDLVGYGSASDFEG
                     AGSAAGLSNTSAALRADGSCTDTGDNATDFAADTPTPRNSASPEHSCAGTPSSGPTGS
                     VNVGLDVASVLSVSLDRPSLNFGTFTSGDRPAPLPERVTVSSNNTAGYALVVARTAFA
                     PTDLPLALSATAPAGGVLGGGLTGGALVPIPISPATGLTVGTKAASSAAGGDAWATNI
                     GFSSPLPLVATGHYAATVTFTAVAR"
     gene            20236..20982
                     /locus_tag="VF225_09310"
     CDS             20236..20982
                     /locus_tag="VF225_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145489.1"
                     /translation="MRQVLALAGLTALVLAPAPSAKGAGADRSRVALSVSPARVALAA
                     PGSKTIKLRNDGAERVAVDATRRTLDRQTVARTWIQIVPAHLLLRSGESAILTLRAGP
                     PRRAEPGNHQLLVLLTTRPLRGARVNVQVRLGVRVRMVVPGRIVRHVTLGGLSLHRRG
                     NARLMVVSIANRGNVTVQLRGRVTALLVRRGHQLARLSPPARRALLPGARAVLALRYR
                     GRLRGPVTAIVRVRLGPGIRVAERRYRIRL"
     gene            complement(20977..21591)
                     /locus_tag="VF225_09315"
     CDS             complement(20977..21591)
                     /locus_tag="VF225_09315"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX7145490.1"
                     /translation="MKTLEIETPSGTANAHVNSAAEPRAALVLGHGAGGGVQAKDLAA
                     AARVAHSVGMSVALVEQPYRVAGRRTPAPVRQLDAAWTAVIDHLLAGELRGLPLLVGG
                     RSAGARVACRTAEAVGAIGVLCLAFPLQPPRRSGATPAQSRLAELDAVTVPTLVVQGA
                     RDPFGMPPATEPRTVVQVAGDHSLRTDLAAVGEAVRAWLHRVVS"
BASE COUNT         3510 a         7428 c         7089 g         3748 t
ORIGIN      
        1 gcaagaggac caccgcgccc gtgaacgtcg ggttcatcgg gccgccaagg gcgtgcaacg
       61 cgtaccagcc gaggaacgcg ccgatcatgt agacgtcccc gtgggcgaag ttcaggagct
      121 tgaggatccc gtacaccagc gagtagccga gcgcgataag cgcgtagagg ctgccaagcg
      181 tcagcccgct gaccgtcagg tcgaagaaca ggctccagtt cacagagacc taatctctca
      241 tagactagtg cggcccgtca cgagacgggc cgcacttgga gttggatttc ccctcccgtt
      301 agccggggat cggaacgaag acggtgccat tcgacttgta gaggccgaac ttcttcgccg
      361 tcgaaagatc accgttgcgg ttgaagcgga gcgcaacgcc gatcagcgac tgagcggcgg
      421 ggatgttggt cgccctgatc gccgcccgga ccgatgcgcg cgtggccgta cccttcttgc
      481 acgcccggtc gatcgcagtc gcaaccactt gcgtggccgc gtacgtcggg gcgccgaagt
      541 attcgccgtc cccgccgtgt cccttcttga agcccgcgat gatcttcgac ttcgggttca
      601 aggggaagtt cgtgtcgtac acgtgcgaac cgacggctgc gaacgacgaa tcgaagagac
      661 cgtccgctcc catcagcttg atgcttccct tgccggcagc cttcatctgg ttgccgaacg
      721 cgatgccctt cggcgtcagc tgccacggga tgtacacgag ctgcgtgtcg cgcgcgatct
      781 tcgcgatcag tgacgagaag tccgactgct gctggctgac gccgtctcgg ccgacggtga
      841 cgcctgctgc cttcagcttg gactggacct cgtcggccag gcccgtgctg tacgtctcct
      901 ggtcgtcgat gatgtagaca cgcgtgtatt tcaggacctt gatgatgtag ttcgcagccg
      961 tggggccctg gatcgcatcc ggtgggactg tgcggaacat gtaaccggtg cgggtcccgt
     1021 ccgtcgtgag cgacgtccga gtcgacgaac cggataccca cgcgaggcca ccgtccatca
     1081 gagacttcgt cactccgacg atctcgttgc tgccggcggg tccgacaact ccgaggacgt
     1141 tcgggctccc ggcgagcgcc tgtgctccct tcaacgcttc ggcggtccct gtcggggcgc
     1201 ccagctgcgt gtcctccgtc tggagcttca tcttggtctt cttgtgcgtc ttgttgtagg
     1261 tcgagacgta gtactgagcc cagtgcacct gttggatgcc gatggacgcg gcgggacccg
     1321 tcaccggtgc catgaacccg atggtgcggg tcttgccgca cttgatcagt gctgagtcac
     1381 tcccagtttg tgcagtcggc gcggccgccc gttcggtggt cgcataagct ccggccacga
     1441 ccgcgagtgc ggctccaagc gccagcaagg ccgcaaccgc agttgccctt gtcctcctca
     1501 ttcgatctcc tccttggtgt ttaccccggt cctcccccac ttgtgacgac attcgagcct
     1561 acttgttgtc tacatacgtc cggttggccg ggctccccga gggatggaac acgccgagga
     1621 tccgcagcgg ggccgagccg gtgttctcga tgcagtgcac ctcgtccggt gggaggtgga
     1681 agcaagaacc cgttttcagt gggaactcgg cgccgcccac atgggcgatt ccttctcccg
     1741 cgacgatgta gccgacctcg tcgtaggtgt ggctgtggaa aggcgccttg ctcggctgaa
     1801 cgattcccag aaactgcgtc acgtcgaagc agccggcgtc ctcgttgacg agatatcgaa
     1861 agctacgctc gaccgacgcc tcgagctcgg gctggtcgtc gaagcggacc gtcaccttcc
     1921 gctcgccgga ggccgccgcc cggtcctcgg gagcgatgac cgacaggacg tgcaggtcgt
     1981 gcggccccgg gttctcgaca gcgtaggtct ctccgggggc cagatagacg cccatgttgg
     2041 gctcgagctc gtgcgtgttc ccgtcgacat gcagctgtcc acggccggcg acgatgtaga
     2101 gaacctcctg ctgtccggcg agggttcgtt cgactgagcg gcccggcccg aaccgaatga
     2161 cgcgctgctc cagacgctcg cagccgttgg cagcgtcgaa ggcgatgtaa acgctggcgg
     2221 tgtcgccgtc gtcctgaatc ggctcgaggt cgttctcgtc gattacgaag ctactgacag
     2281 cggccatcgc tggattctag gtcgtttggt cgagcaggct gagcgcgagg acctcgactc
     2341 cgtcgccaac ggtcacccgg ccccgctccg tgacctcacc gtagacgccg aaggggatat
     2401 gtctcttgcc cgcctcgttt tcggtgaagc ctcggtagct cgagatcgtc cgcagggtgt
     2461 cgaaatcagg ctcgccggtg tccggattct gggtcgtgat cgcgcagcga ccgacgtccc
     2521 cgcggatcct gatcgttgcc tcgccgatcc tcaggtgccg cttgacccac ccgtcctcct
     2581 cgtgcggcgc gcagccctcg agctcgaaga gcatccggaa acggcgcccg tcgacggggc
     2641 ggccgtggtc tccgttctgc ccgagctcgg cgagtgatgc agccgagatc agtgagacat
     2701 gaccgcggcc acggtcgacc gcgaggccgg gcggcgactg gacgaggcga agctgcacgc
     2761 ccgcccattc ggagagcgcc ccggaccagg ggccctggac gtagctgccg acgactggtc
     2821 ggccgtagaa gtcggtcgtg acctccgtcc cgagcgaaac ctcgccgtcc gcgaccgacc
     2881 cgtcggggaa gcgcagtgtg agccgttcgc tgtcggcgtc gtactcgggc aggatctgaa
     2941 ccagcgcgcc gttccgcaac tgattgaagc gccgcccgtc ggcgtcgacg atgtgaaacc
     3001 ggcggttctc ccgaacaccg tgacgttcga gcgagacgga ctcggggtga acgagcccca
     3061 accccttgac gggggcgacc gagatccggg tcacggctgg catccagcga gcgtaccggt
     3121 gccaggcacg ggtacgaaac ggttgcagaa gcgaaaacaa tcgcgcgccg cgacgagttg
     3181 gcgacccgcc ggacggcatc ctgacgagat gccccgcccg ccgaggaacg cgtacccggg
     3241 tggtttcttc cacgtgggca cgcgcgggaa cagccgctgc cgcgtcttcc tcgatgcgac
     3301 cgactacaag gtgttcctga tgatgctcga tcgcagcgag agaaagtaca actggcgcgt
     3361 ctattcgtgg tgcctgatga ccacgcacta tcacgtggtc gtggcgactc cggacggcgc
     3421 gctttcgcag ggtatctgcg agctcaacgg aagcttcgcg cggtggtcga atctgagaca
     3481 cgaacgcgaa gatcatctct tcggcaagcg cttcggcagc aaggagattc tgacgaacga
     3541 acagctcaag gaggcctgtc gctacgtcgt tctgaatcct gtccgcgccg gcatgtgcga
     3601 agacccggcg gactggcgtt ggagcagcta ccgagcttcg gccggcttcg acgaggcgcc
     3661 gccgtacctc gcgctgggtg agctcttggc gctcttcgac gtcgatcccg gcaaggccat
     3721 ttcgctctat cgtgacttca tcgatcaggg gctggcttct tgagacacgt accggtgcca
     3781 ggcacgggta cgttcctgta acgcgtcacg cacgcgtttc gacttcgacg tcgtgaccct
     3841 ccagctcgaa gacgtagaag tcgcggcccg tgacgtggac ggcgacgttg tagaccggta
     3901 cgccgccgat cgcgccttcg aagctgccgc tgtgggcgtg gccgtgcagg acgacgtccg
     3961 gacggtactc ggcgatgggg gtcgcaaggc ggtcgctgcc gagatacgtc cagatcccct
     4021 ccggctcgcc gtgcaacgtg tccgacgtcg gcgcgtagtg caggagcacg atgcggatcg
     4081 ggcaatgcgc gatctcctgc aggccgcgac ggatggcatc gacctcttcg gtcgtctccg
     4141 cgtatacccg gcgaagcagc ggttccccga agtccggcag ctgcgagccg ctgaaacctc
     4201 cgacgaaacc cttcgtaccc gccacaccga tctcccggcc gacgagctcg agcacgatcg
     4261 cctcgcgatc gagcatgcgc acgccggcct caaccaggat cgatgagatc tgctcgccgt
     4321 ggccggaatg gagatcgtga ttcccgagca ccgcgacaac cggcacacgc aggctgcgcg
     4381 tcgcctcggc gagcgcctcg gcctgctcgg gctcaccttg cgtcgtcaga tcgccggcca
     4441 gcaggacgag atccgcctcg cgctcgacgg cggcgaacgc ctccgcgata cgcgaacggg
     4501 tcgcctcgga ggcgtgcaca tccccggctg cggcgatcct caccatggct tgatgttcca
     4561 catccggccc ggacgggaaa ccgccccgcg tgaacaccga ccaggaacgg gaattcgagc
     4621 ggctcctggc cgcgatgaag aaggcgggcg gcgccctgaa gggggcggga gtcccattcg
     4681 tcctgggcgg cggcctcgcc tgctgggccc gcggcgcgcc gaaaacagag cacgacgtcg
     4741 actttctggt caagcccgag gatgcggagc gcgcccggag gacactcgcc gacgcaggga
     4801 tgcgcaccga gacgccgcct gagggctggc tgctgaaggc ctacgacgac ggcgtcctga
     4861 tcgacctgat cttcgaccct cacgacggcc cgatcgacga cggcaccttc gaacgcgccg
     4921 aagagctcga ggtccacgcc atgcggatga aggtggccgc actcgaggac gtgctcgtac
     4981 agaagctcct cgcgctcaag gaacaggagc ccgacttctc gagcgtcctc gagctggctc
     5041 gcgcgctgcg cgaacaggtc gactgggacg aggtgcgcga acggacacga gagtcgccgt
     5101 tcgcgaaggc ctatttcact ctgctcgacg agctcgagat cgttccggcg taactcgcgt
     5161 cagaagtaga actggctcca ggccgaccac cagtcgtcgg ccttcgacga gagcgtcagg
     5221 tcgttcacct gctcgacacc gcggcggttg cggtactcga ccactcggtc gggcgtcatg
     5281 aactcgatcg tgatctcgca gggacggccg gggtcatacg gcttcacggc cgaaagatcc
     5341 cgcagcgcac gcctcacacc gtcctcgatc atctcccgcg cgcgcaacgg cgggacttgc
     5401 cgggcgctga agcggccgag gcccttcttc accgcgaccg ttgtcagccc tgatccgagc
     5461 agctccgcgc cctcgcggca caccgcctcg tcgcctgtga cgagcaacac tgggcagccc
     5521 cactgcccgc agagtgccgc gttgatcccc gtctctccca ccgggacgcc gttgaagcgc
     5581 aattcacgcc aggcttgccc ggagaccgta tgactcagga ccccgtcagg cgtaccggct
     5641 ttcgcgtgca tgccgacgaa gagagcggcg tcgcagccct gctcgagaaa ctccgtgtat
     5701 tcggtccagt cgttctgcac cacgtactcg caaccgcgat cgagcttgtc cggaatgagg
     5761 gagttgaagg tccactcctc gccggcgccg tggcagtcca taacgaggat ctgagtcgca
     5821 ccgccggtac gggcaccccg cacggccgcg ttgatctcct ccgtatagag ctcgcgtcct
     5881 tcgtgatagg agcgcttctc cttcgtcccg tccgtctgct gccacttgac gatcccggcg
     5941 acgccctcca tgtcgctgac gatgaagacc ttcacgaacc ggcctggccc gccgcaacag
     6001 gagcgggcgt gctccacggg aagttgatcc agcggtcggt ccgccgccag acgaaatcgc
     6061 agcggacgac actctgcggc ttctcataga gaactgcggt ccgtacctcc ggaccgtgtt
     6121 gcaagcagaa ctcactggcc agcgccagcg tgcggcccgt gtcagcgacg tcgtcgacga
     6181 cgagcaaccg ttcggcacga agcagggcga gatccggcac cggcggcaac agcagcggct
     6241 cgtccaaccg ctcgtcgacg ccggtgtaga actcgacgtt gagcgtcgcc atgttcttca
     6301 cgccgagcgc gtacgcgagt gcgcccgcgc acgccaggcc accgcgggcg agcgcaagca
     6361 ccgcgtccgg accgaaaccg tcagcccgga tctgcgccgc gaggtcgtcg accgccgtgc
     6421 cgagatcctc ccaggtcatc tgctcgcgca taacagggag aatcccacag atggaaacgg
     6481 ggctggcggg gaagcgcgtg ctcgtgacag gagcctcagg cgggatcggc tccgcctgcg
     6541 cgcgggcctt tgccgcggcg ggagcggacg tggtcgtgca ctaccaccgt ggcaaggagc
     6601 gcgcggagca gctcgccggg gaactgggcg gtggcgccgc cgttgtcggc gccgacctga
     6661 cccgggagga tgaggtcgac agcatgttcg ccacggccgg cggcctcgac gtctgcgccg
     6721 cagtcgcggg cgtgtggccc tcggaggacc tgcctgtgtg ggagctcccg ctcgaacggt
     6781 ggcgcgccac gctcgacgca aacctgactg caaccttcct cacggcacgc ggcttccttg
     6841 gccagctcga aggcgacggc gcgctgatcc tcgtcggctc gacggccggc atcgtcggcg
     6901 aggcaggtca cgccgactat gccgccgcga aatcggcgat cctcggcggc ctcctgctca
     6961 gcctgaagaa cgaagtcgtg cgcaagagcc cgcaggcgcg cgtcaacgcc gtcgcgccgg
     7021 gttggaccga gtccgcgatg acccgaggac atgtctcgaa ggaacaggta cgccgcatct
     7081 cccggacgat ggcgctgcgc aaggtcgccc aacccgaaga cgtcgccaac caggtcgtgg
     7141 tgctggcctc accggtcctg tccggccatg tcaccggcca ggtggtcacg gtcgccggcg
     7201 gcatggaagg ccgagtaatc cacgagacct agaaatgaag gtggcggcgg agtcctcgtc
     7261 cgccgccgcc ggtactccgc aagccgcagg gatgattgcc gtcagtccct ggccctggag
     7321 tcaagacggg aatcggcagg agatccttcc ccctttagcg ccgcccgcga ggcgaaatct
     7381 tccgtgaacc gggccagtca accgagaacg ggtgatggat caagtgcccc ctccccgcga
     7441 tagtcagaag gcataaccga gatgggcccc gatgcggcgg gggcaacctc ggaagatctc
     7501 cttccatagg agacccagga ggggccaggc ggcggcccct cttgcgtttt ctggacacgg
     7561 gtcgcgacgg gccccctgcc tatactccgt accggaagcg ggcaacgtcc cgctccgcat
     7621 cgagagaggc tgagggaacc ggccctgtga agcctcggca acctgccttc gcggcaaggt
     7681 gccaaacccg gccgggcgca agcccggggg gatgtgtcga gcgagttcgg aaggagacga
     7741 agtgcctgcg caaagccaga cccgcaaccg cttcctagag cgcctccaga gtgggcccgt
     7801 gatcgtgggc gacggcggca tgggtgcgct gctttccgca gcggtccctc ggctccgtag
     7861 cccggaggag gcgaatctcc gggcgcctga cgccgtcgtg tccctgcacg tcagcttcat
     7921 caatgccggc gcggagctga tcgagacgaa cactttcggg gcgaaccgtc gcaagctttc
     7981 acaccatttc ctggaggacg atctggagcc gatcaactcc gccggcgtca agctcgcgcg
     8041 cgacgccagg gagatgaccg ggcgcgacgt cttcatcggc ggatcgatcg ggccgctcgg
     8101 cgagccggcg acctcgcgag tacgtcgcga gctcttcacc gagcaagctg ccgtcctcga
     8161 cgggcgcggc gtcgacctct tcatggtcga gacgttctac gacttggaag aagcggtcga
     8221 cgccatcgag gcagtgcgca gcgcgtcgag cctgccgatc gtcgcactcc tgacgttcga
     8281 cgagagcgcc gaaacgcttg ccggcgtgac tgccgccgag gcagccgcac ggctgacgga
     8341 gctcgacgtc gcagcgatcg gcgccaatca cggcgccggc ctgctcgccg ccctcgccgc
     8401 cctcgagcag atgggcaaga acggcaagcc gctcgcggcg cttccgaacg tcggccttgc
     8461 gagcttgacc gccggccgcg tcatctaccc ccacgccacg ccggagtact tcggcgagtt
     8521 cgcagcacat gcacgcgatc tcggcgcgaa ggtgatcggc ggttgctgcg gtacgactcc
     8581 cgccgagatc gcggcaatcc gatctgccgt ggaggacgag cgcaagccgc gagcgccgct
     8641 cgtgttcgac actccggagc tcgtcgtcgc gctcggggag gaacagaagg agacggggct
     8701 ttcacgtgcg ctggcagcag gcgagtgggt cgtctccgtc cagctcgacc cgccgctcgg
     8761 cggtaacagc gcagggctcc tcgaggttgc gagcacgctg aaggagtccg gacgcgtcgg
     8821 ctgggtcgat gtcaacgaca atgccacggc tcgtgccgga atgagctcgc tgatggtctc
     8881 ggcgacgatc gagcgtcgtg ccgaaatcga gacgatcccg cacctgacga cgagggattg
     8941 gtcggtgatg ggcctcgagt ccatgctgct gggagcgcag gccgagggtg tgcgcaacgt
     9001 gcttgcggtc accggcgatc cgccggaggt cggtgactat cccggagcgc gcggcgtgta
     9061 cgagatcgat tcgatcgggc tcacccagtt gatgacgaac ctcaaccgcg gcgaagactt
     9121 caacggccgc ccgatcgatg cgccaacgtc gttcttcccc ggggtcgccg tcaatccgac
     9181 acccgacgac ctggacgtcg agctggaccg cttcgagaag aagctcgaag cgggcgccaa
     9241 gttcgcgatg acgcagatcg tgttcgacgc cgaccctctc gaccgcttcg tcgagcgact
     9301 gggcggcaag tggccgatcc cggttctcgt cggagtcttt ccgctgacga gccaccggct
     9361 cgcgctgcgc ttgcacaacg aggtgccggg catcctcgtt ccacaagcct tgcaggacga
     9421 gctcgagcga gcaggctccg atgccgccga cgtcggcttc gctcatgcac gcgaattgat
     9481 cgccgcttca cgcgagcgcg ccgcgggcgt gtatctcgtc gcgccgttcc gtcggccgct
     9541 gcgcgtcctg gaactgctcg tcgactagat gcggtagcgg aggtagacca cgccgctacc
     9601 gaaacgacgt tcgtcgagca attcgagctt caggcggaca tcatcgggga gcgaccgttt
     9661 gccgcctccc gccacggcgg gcgcgacgaa caactggagc tcgtcgacca acccggcccg
     9721 gatcgcctgg gcggcgaggt cgggaccgcc cacggtgatg tcacgttccg ctgacgcttt
     9781 cagctgccgg accgtctcag ggtcgaagtc tcgctcgatc cgcgtcctcg cggtggccgc
     9841 cgtctccagc gtctttgagt acacgatctt gtcggctgcc tgccagatct ccgcgaagtc
     9901 ccggacgaac ggcggctggt ccgggagggc gtgcgccgtc tcccagtaag ccatcacctc
     9961 gtacattcga cgcccgtaca ggtacgtacc gacgggccgc tcgagatcgt tgaccaagcg
    10021 gtgcacctcc tcgtccggct cggcccagtc gaacttgccc gcttcgtccg ccacgtaccc
    10081 gtcgagcgac gcgatcgccg agtagatcag cttgcccatg ctgagcctcc gctagggagc
    10141 gcagtcgccg gtcgagaccg gctggcctgc tgcgtcgaga tcggcgcgaa cgacgtcggt
    10201 cgggactccg aaacctcctg tggagcggag acgagccgca aagatcgtcg tctgcacggc
    10261 gccgtgcgca tcgaccgccg gcgcgcccga atccccatgc ctgacgcgtc ctcgcaacga
    10321 cgtgatcctg cgcgtcacag gtcctgctcc atacgcgtct tccgccagca ccgtcgacgt
    10381 gtggccgata cgcgcaggag tcgcggtgaa aggcccgttc tccgggaagc ccagcactgc
    10441 aacgggagct cccgacctcg cggagaccag cggtagagca cgaagcccca gctcctgcac
    10501 acgcagcact gcgacgtcgt tgcgacggtc gaacgcgacg acgtccgccg gcagtcgccc
    10561 ccccgattgc agctcgacca cagtgtcgtt ctgtccggcg accacgtgag ccgcggtcac
    10621 gatcagattc ggtcgcgcca cccagccggt gccctctaca ccaactccgc aagccatccc
    10681 gagcacgcgc accacgctgg gcgccgcacg gcgaacaccg acggcgtgcg cgaggcgcgg
    10741 atccggcggc gcgaccgtcg ccgacgggcc ggtgatcgcc ggaaacgggt cgatgcgcgc
    10801 cagcgcattc aggacttctg tcggcgggat gaacttgttc agccgttgca gcaaagccga
    10861 gccttgtgcg ctgcgccgaa ggtccgtctg ccctgggagc aggagcgcca ccgccccgag
    10921 cacccacacg accgccagcc cggcgagcgc cccgaggacg agcccgcccg tggcgtcgaa
    10981 cgtgctcagc ttcggcctcc gcaccttcga gcggagcgcc gatccggcca gcgttccaag
    11041 cgtttcgagc agaatcgcgc cgacggccgc gccggccaga gccgccagcg gcgtgtaggg
    11101 agaatgagtt ccgtcctgga gaagctgcgg cgcaagcctc gatcccagcc aggcacctaa
    11161 gaccacgccg gcgagagaca gagcgcttgc gatcagcccc ttcttccagc cgatgaaggc
    11221 cgtgaggaca acgaagccga ggacgatcag atcaaccttg gtcactatgt cggcaggcta
    11281 ctcgcggtct cggatctctg ggaagcgagg tagactggtc gaccactggg ggcgtagttc
    11341 agttggttag aacgccggcc tgtcacgccg gaggtcgcgg gttcgagtcc cgtcgctccc
    11401 gctctctcgt ttcgatccgc tcccgacgtg agccgcggcc gcgcatgtcg gccacggcgg
    11461 cgattgctcg gctctctgcg accccacaac gcgattgcgt ttgccgtcgg gtcgtgcacg
    11521 aggaacgcgt cgccacgtct ctcggcgttg tcgagcctgt cgagtccgtc gctcccgctg
    11581 tttactcctc agcctggtgg cgcaaactgg caactgctgc gtccccgact ccggcggcgg
    11641 acccggcggt accgaaggtg ccgggcggat ttcaggcttc gaagggtgaa cgttcggggc
    11701 accgcgatcc tgtcttgttg cgggctgttg gacgcttcgg cgagctcgag cgaggcgcgg
    11761 ccgacacggc cgcgcctcgc tcttgagaat tagctgactg gtctgaacgc gtggttgagg
    11821 ccgtagcggg cgaccttgag tccgagatgg acgcctacct cgctggaccg gcggtagtgg
    11881 atgccgcccc agagtctcgc gttgatgacc tcctcccgga gctggtctgc cgtgtcaaag
    11941 tggtgcacag catccaggtt cccagggagc ccggcggcgt cgaagccgtg gatgtcgaga
    12001 tcgatgtcgt cagtgcccag gaattcgcta aagacctcgg ccatggcgga cgtattcgtg
    12061 ccgtgcgcgg ccgggtactc gggatgatta ggcgtcgtga ccaggggccg ccaaccgggt
    12121 tcttcaatag tcgcggggtt gccgtcatcg aacccaggta ccggtccgaa gccgtctgcc
    12181 gtcaccgcgg tcggatcgat cgcggtcaca ggccgccaga agaggaatcg gtacttggcg
    12241 ttgagcacag acatcagagc gtcggcgctg acggtgttga ccatggccac cagccgtgcc
    12301 gtctcgagtt cgcctagccc acgggcggtg gcgaggtcac gagccgcccc gttgaattgc
    12361 cggatcacat tcgccgtata aaaccttgca gtcgcagtct cgtccgaggt acgaaccgta
    12421 ctagtcaccc cgccaaaggc cttgacctcg ttgaactgcc gaacccactc tgtgctggag
    12481 agcgggatcg gaggttcagg ccggaattgt cccgggctct tcagcaggaa gggttgtacc
    12541 gacccgagcc acggcgtttg cgggaccaag aacgcgggtg gtgtaagacg ccacactcct
    12601 gggccggggg tcttcgtttc aaacgacgac gtcgtaccaa ccggtgtcat ccgcccgtct
    12661 ccggtacgaa gggcgatgat gctgttcgcg gccgccaatc cgacgctcgt tcctttgccc
    12721 gcatcgcctg tgcatccgtt caagttcgag ggtgacagtg cctcggagta gtacccgtcg
    12781 aggctcgcca ccagcgccgg gaatgtcgag aagtaatggc gcagcgtcct ataagcagct
    12841 tcgacaaccg cgcaatcgag tgaagctccg gtaggtgcac tgatcgcagg tccgtacggc
    12901 tcgaacccgc cctgaatcgc gacgaccgca tcgtaaacgg ccaacgactc gtaacccata
    12961 tagatctcgc cctcgccctg gaacgctccg gacgcgagca ccgtatcctc ggcgatcttg
    13021 ttccactgct gaactgtgtt cggtaacggc ggaaggaccg cctgagcgtt gcggctggta
    13081 gctgccccga ctgcggcgac cactgcaacg ccgacgacca gtcccaagat ccgcgtactc
    13141 agcgacgtca tcaagtctcc cctcgtcgac cgatatctgg agatacggaa cgcgttggcg
    13201 gagctcaaca tcgaggaaca ctcgccgcct gcgccctcaa cgcgttcgat tgagcaaggg
    13261 tgcttctcga acgaccagct cggcatccct tgctcggcgt catcgtgtca caacgtggca
    13321 gacattacaa agccggcgca acacaatgct tccggcaagg gactttgatc gacagccctt
    13381 ctggacactg gtgctgtacc cggaggccgg ggaggcgggc ggcacgacga tggggctacc
    13441 ggagcctcta gcgccaaccc aggacgccta gcggattgac ggcggcccct cgcgtaaccg
    13501 gatcacgggg ataaagcgcg agttcgagtc ccgtcgctcc cgtgcttcag gggaggcgcg
    13561 gaggccgcag ctttcagcgc gcccgcctgc tcatcgcgga gcccgcgagc gcgctgcggg
    13621 gcgcaggcgt catcgtccgc ggttccgccg tcgctgcgag tgctcgaact gttggaatag
    13681 ctggttgatg ggcgcggcac cgcgttatgc ccgaccgttc gtcgccatct tggtcgggat
    13741 ggtcgtggta tgtgcgctgg cgcccctgaa cctctggccg ttttcgaact gggagctctt
    13801 cagccggctc cgcggccccg tcgagagcta ctgggaagcc gtcgtcgagg accacggcct
    13861 tgtcgtcgcg gactacacga tcggaccgca cacggcgtgt gtcccgcttc tacgaagcgc
    13921 aacggctcga ttcgggccgg cgaccgacgt gcggatctat cacgtcgaac gtctactctc
    13981 ggaccgcctc ggctctcaag cggcgcctcc ttaccggacg ctcgtcgcca cgtgcacggc
    14041 aaaaggcgta cgtgcacaga gttgagcgct ggatcttcgc gccggaagac gccaggcgcc
    14101 tcgccggggt tcgcatcggg atctgcctac tgctcgcgta ccgcctctcc gcccacgact
    14161 acgacgcgct cgctgcacgc cacgtccgct tcctgccgca cttctacatg gacgtcttcg
    14221 cgcacatgcc gtcccccgac ctcgcttcgg cgttgcagac cctcggcatc gccgctgcgc
    14281 tcgccgccgg cctaggcctc gcactccgta tcactcttcc ggttgccctc gtctgtgggt
    14341 tgatcctgaa cgggatgctc aacagcacgg gccgcgtgat ggtacgcgat gcgctgctga
    14401 tgctgtgcct cgcggtcatc atcgccgccg gacgcgcagc cggcgacgtc tgggccgtgc
    14461 gcgcaccgaa ccagatggcg ttcggcgaac gctacggttg gccgatccga agcgcgatga
    14521 tcgtcatcgc gctcgcgtac ttcttcgctt cagttcagaa gtggcgctac tcgggattcg
    14581 gttgggtaac aagcgacaat ctccgctgga tcctgtacgc gcaaccgcat ccgaacggct
    14641 tcgcactgtt catcgccgac cgtcctctgc tcgcgcacct gtttgcggcg ggcgcgcttc
    14701 tcctcgagac gtcctttccg ctcgtcctct tcgtgccgag actgcgttgg ctcctaattc
    14761 cggcggtgtt gacgatgcac gtggcgattc gaatcgcgct tggcctcgat tactcggcgc
    14821 aagcgctgac tgtcctgatc gtgttcgtca attggccggt cgtcgtcgac tggacccgcc
    14881 gcacactggc acccaccccg gccccgcgag agatcgctcg ttgagccgta cggcgcagag
    14941 tgtcctgctg tacgacgagg actgcggctt ctgcaggtgg tcgctcgaca agatcctcgc
    15001 ctgggatcgg gggaagcgcg tgcgttcggt tgcgatccag agcgaggagg ggtcgcgcct
    15061 gctcgcaccg atcgctcccg acgcacgtct tgattcatgg catctagtcc gcgctggcgg
    15121 gaggctcttc tcggcgggcg cagccgccgg acccctcgcg cgactgcttc ccggcggccg
    15181 cccgctcgcc gccctcttcg cggcgtttcc cggtgtcacc gagctggcct atgggttcgc
    15241 cgcccgccat cgcttcttgc tggcgcggct cctgcgagtc gacgcaaact gtcaggtgcg
    15301 tcaccgtgcc tgaacagacg tcccgccgcg cgaagatcgc gctggcaact ctcgtcgccg
    15361 cctacctcgc gatggcggtc gtcgcaggcg ctccgaactc gccgctcacg gttctgctgc
    15421 cgaacggcgc gacctctccg acctgggcgc gtttgcttgc aaatgcagcg agcctcgccg
    15481 atgttggacg ccgaggactg acggtggtcg cgtgggtgct cctcgtcgtc gcggtcgcgg
    15541 cgttcgcggt cgtggtcctc gaggcgtggt cgaggaggat ccgccttgcc gccgtgctga
    15601 ccgcagcgtc ttgcagcctg ctcatcgtcg tcgcaggtcc gctgctcctt tcgcgggacg
    15661 tctacacgta cgcggcctat ggacgcatcg aggcgctata cggcggcaat ccctacttca
    15721 cgactctgtc ggcattcccg cacgatcgct tcgtcgccgt gacgtccgtg caatggcttc
    15781 atacacgctc gcactacggt ccgctgttca cactggcaag cgcagggctc gcgcgcgcgt
    15841 gggcgggttc ggtggatgga acgatcctcg cgttcaaggt gctcgcagga gtctcgctcg
    15901 cggctgccac cggtcttgtc gcactcaccg cacggaagat ccgtcctgaa cgtgcagcgt
    15961 tcgccgtcgc gctcgtcggc ctgaatccgg tggtcgtcct gcacacggtc ggcggcggtc
    16021 acgtcgacgc gccgattgct gcgctgcttg cagcagcgtg cgcatgtgtc gtgacgcggc
    16081 cgcgcggcag gtcggcgcgc gcgtttgcga tcaccgtact gatcacgctc gcctgcctga
    16141 tcaagactgt gatgctgccg gtactcctgc tgtggtttgc gtggctcgct cgtccgcgcg
    16201 gcgtccgaac tcttgtactc caccttctcg tgatcgctgc gctcatcgtt gcggcggtag
    16261 gagcattctt ctcggtgtcg cacccgacgg cgccgttcgc gagcttggga ggaatcgagt
    16321 cttgggccag tccctcgcat ttcgtcgccc gagacgcgca gacgttcgtt gctcttgttg
    16381 ccggcacgca agcaggacac gacgcaagcg tcgtcgcgga agcggcattc ccgctgctct
    16441 tcgccgtcct cctgtggcga ctcgttcgac gcataaggga agcgggcgcg agagcgccga
    16501 tcgcggaatg ggggatcgca ctgttcctga tcgcgttgtc gcttccgtac ctacttccgt
    16561 ggtacgcggc gtggttcgtt ccgttcctcg cgttcctcga tgacgccgtg ctcacattcg
    16621 cgggcgtgtt cgcctgcgtc gtgctcgctt ttacgctcgt tcccgcggat ccgtttcacg
    16681 gcttgacgac gccggcagtg atggacggag tccactacgg cgctgcgccg ctcctcctcc
    16741 tcgttctcgt cgtcatcgcg gtgcgcttgc gacctcccgc gtggggaggc gccgaacgtc
    16801 atcgccgggc ggtcagggtc ttggcggccc tcttcgcaag cggatcacgg gggtaaagcg
    16861 cgggttcgag tccatgatga cttgcgagga ctcggtttcg attcgaggcc gtagcggccg
    16921 cggccgcgca tgtcggccgc ggcggagact gttcgtttct cgccgctggt ggcggcgcga
    16981 agatagccga catgacagag tgcacgagac cgaccgaggg gagggtgacg cgtgaaccac
    17041 aacgcgagca tgccgcggaa ggagtttctg acgaaaggcg cagctgtgct cggcgccgcg
    17101 gtggctgctc cgcttgtcgg cgtcgccggc gcgcaggccg cagccgagcg cgcgctcgcg
    17161 ggcgtcggcc gcggggagct gggactcgtg ggcttggacc acgtgggtat caccgtcccg
    17221 aacatcaacc aggcaatcga gtggttcgag gatgtgctgg gcggagtcgc gccactgacg
    17281 ttcggcccgt taccggcagg gcccttcgtt gcgagcgtcg tcgacgtcgc ggagacgacg
    17341 tcgatcgacc agatcacgat gcttcgcatc ggccatagcg ccagcatcga gctgttccag
    17401 tacaccgcgc cggacgggca gcggcacgat atgccgaaga actccgactg gtccggccac
    17461 cacgttgcct tctacgtcac agacatcgac acggcggtcg agtacatggt cgggaagggc
    17521 gtgcgcaaaa tgccggcagg cccgttcgcg ctcactcagg gaccggctgc cgggcagacg
    17581 atccagtact tccagacgcc ctggggcggc tacatcgagt tcatcagcta cccggatggg
    17641 atggcgtacc aggtgccgag cgtcaagccg ctctggtcgc cgaagctgaa cggtgccgac
    17701 tcagtcgtca cgaacgtgcc cgggctgctc ggcatcgacc acgtcggctt caccgttccg
    17761 aacgtcgacg tggcggcctc gtggctcgaa cagaagcttg ggttcagcaa cccgctcaca
    17821 ttcgggccgt tcagcgatcc gaccggcgat ttcatgacgc aactcgtgga tgtgcacccc
    17881 cgtgccgtgg tgcagcagat ccgcatgctc cgcggcggca atgggccgaa catcgagctc
    17941 ttccagtaca catccccgga tcaggatcaa acgttccggc ggaactccga ttggggcggc
    18001 caccacatcg ccttctacgt ccgccacatc gacaagggcg tcgagtacct gcagacgaac
    18061 gcaggaacga aactgttcgg cccggtcacg ctgacggacg gccccgcggc cgggcagtcg
    18121 atcaactact tccacgaccc gttcggaacg gacgtcgagc tgatcagcta cccacacgga
    18181 atggcatacg aggagaccgc ggcgatccca ctctgggatc cgctcgacaa ccacccctag
    18241 cgacgcccga acggaacggt tagcgccttg acggcccgcc tcggaagcgg atcacggggt
    18301 gcatcagctc cgccgatcgt ggggtaaacc gcgcatgtca gccgcggcga acgcggtctc
    18361 tctctttgtt tcaggccttc gtggcggttg tagctgatgc cacgagacca aagggggtag
    18421 acagagctgg ttttctcact acgatccgac gcttaagaac aggcctaggg ccgcgcactc
    18481 acctgatgtg cggcccgtcg ttctctctcc tccatgccga ccgccttccg cagccccctg
    18541 ctgacgttcg tatttgtcgc gatcgtcttc ggctgcgcct ccgctttccc gcacgaggat
    18601 gcagccgcgg ctgtcgctcc gcagacattg actcggcacg atccgatccc tgcggttccg
    18661 aagccgaccg tcggcgagcg cgcggccgcg atcgcggtca gagcggtcgg cgtgccttat
    18721 cgatggggag gctcctcgcc tgccggcggc ttcgactgct ccgggctcgt cgactgggcc
    18781 tacggccggc tcggcattga gctcccacac agctcgtacg cgctttacga ccagggccgt
    18841 cgcgtagcgc gagccaggat gaagcccggc gacttgctct tcttttcagg actcgggcac
    18901 gtcggcatct acatcggcct cgggcgcatg gtgcacgcac cgcactccgg cacccgagtc
    18961 caggtcgtca agctgggacg ctcgtcctac ggggcgcggc tcgtcggcgt gcgtcgcgtc
    19021 atccacacgt agtcgcctca ctcgagcacg gtgtaaccaa agcgcgcaga gattgcgccg
    19081 gacgttcacg gacactttcg ctcgcctccg ccctgatcta tgacgaacag cgattcgttc
    19141 ctcaacgatc ggagcacaca tgtcaagagt cctgctgctc gcattcgtcg cagcactggc
    19201 cctcttcccc acggccgccg gtgccggcgc atcgggcgta gtagtgagtc agatctacgg
    19261 cggcggcgga aacgcgggcg ccaccttccg aaacgactac gtcgagctct tcaacgccgg
    19321 atccggcgcc gtcgacttgt cgggctggac cgtgcaatac gccaccgccg cgggaacgac
    19381 ctggcagacg accgcacttt cggggacgat cgcgcccggc cgctactacc tggttcagct
    19441 cgcatcgaac gctgacgtgg gcgccgtcct tccgtccccg gacgcgaccg gcacgagcaa
    19501 cctgggcggc accagcggga agatcgccct cgttcgtggt gccgccgcaa tcacgtgcgg
    19561 cgcttcggcc gggagctgct cggccgaccc gctcgtggag gacctcgtcg gctacggcag
    19621 cgcgagcgac ttcgagggtg cgggctcggc ggcggggctt tccaacacgt cggccgcgct
    19681 tcgcgcggac ggcagttgca ccgacacggg cgacaacgcg acggacttcg cggcggacac
    19741 gccgacgccc cggaactccg cctccccgga gcactcgtgc gcgggcacgc cttcctcagg
    19801 gccaaccggc tccgtgaacg tcggcctcga cgtggcttcc gtcctctccg tgtccctcga
    19861 ccgtccctcg ctcaacttcg gcacgttcac gtccggcgat agacctgcgc ctcttcccga
    19921 gcgggtcacc gtgtcgagca acaacacggc cggctacgcg ctcgtcgtcg ctcggaccgc
    19981 ctttgctccg acagaccttc cgctggcgct gtcggcgacg gcaccggccg gcggagtgct
    20041 gggaggcggc ttgaccggag gcgctctcgt tccgattccg atcagtccgg cgaccgggtt
    20101 gaccgtcggc acgaaggccg cgtcgagcgc agcgggcgga gatgcttggg cgaccaacat
    20161 cggcttctcc tccccattgc cactcgttgc gaccggccac tacgcggcaa ccgtgacatt
    20221 cactgccgtc gctcgttgag acaggtcctc gcactcgcgg gcctgaccgc cctcgtgctg
    20281 gcgcccgccc cttccgcgaa gggggcgggc gccgaccggt cgcgcgtcgc gctttcggtc
    20341 tcacccgcgc gagtcgcact tgcggcgccc ggctcgaaaa cgatcaagct gcgaaacgat
    20401 ggtgctgagc gggtcgccgt cgacgcgacg cggcggacac tcgatcggca aacggtggcg
    20461 aggacgtgga tccagatcgt gccggcacac ctattgcttc gctcgggcga gagcgcgatc
    20521 ttgacactcc gagcagggcc gccccgacgc gcggagcccg gcaaccatca gctcctcgtt
    20581 ctactgacga cgcgcccgct gcgcggcgct cgcgtcaacg tgcaggtgcg ccttggggtc
    20641 cgcgtcagga tggtcgtgcc cgggcgcatc gtgcgacacg tcacactcgg cggtcttagc
    20701 ctccatcggc gaggcaatgc gcggttgatg gtcgtttcaa tcgcgaaccg cggcaacgtc
    20761 accgtccaac tccgcggccg cgtcactgca ttgctcgtgc gacgcggtca ccagttggcc
    20821 cgcctcagtc ctcccgcccg gcgtgcactg cttcccggag cccgagccgt cctcgcgttg
    20881 cgctaccgcg gccgcctgcg cggccctgtc acggcgatcg tgcgggtccg gctcggcccc
    20941 ggtattcgcg tcgccgagcg ccgataccga atccgtctat gacacaacac gatgaagcca
    21001 ggctcgtacc gcctcaccca ctgccgcaag atcggtgcgc aggctgtgat cgcctgcgac
    21061 ctgcaccacc gtgcgcggct ccgtggccgg cggcattccg aacggatctc gcgccccttg
    21121 cacgacgagc gtcgggactg tcaccgcatc gagttccgcg agacggctct gcgcaggcgt
    21181 ggcgcctgac cggcgtggag gctgcagcgg gaacgcaagg cagagcactc cgatcgcgcc
    21241 cacggcctcc gcagtccgac acgcgacacg cgcaccggcg gaccgaccac cgacgaggag
    21301 cggcaaaccg cgcagctcgc ccgcaagcag atggtcgatc accgccgtcc acgctgcatc
    21361 gagctgacgt acgggtgccg gtgtacgcct accggcgacc cgatagggct gctcgacgag
    21421 cgcgacgctc attcccaccg agtgtgcgac acgcgccgct gcggcgaggt ctttcgcctg
    21481 caccccgcca cctgcgccgt gaccgaggac gagcgcggcc cgcggctcag ctgcgctgtt
    21541 cacgtgcgcg ttcgccgtgc cggacggcgt ctcgatctcg agcgtcttca ccgagatgat
    21601 gctgccaggt cgactcaatc cgggaggagc ggcaccgtga atccctgctc gcggccgagc
    21661 agaacggcgc gcgtcaccgc cgtcgatccg aatcgccggc gcacttcgtc gagcgcgacg
    21721 tcgagggcgc tgccgctgaa gcggtccaga ggcagcacga gctgaagggc agtgt
//
LOCUS       DASVEU010000215        24734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_344209,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000215 DASVEU010000000
VERSION     DASVEU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344209"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VF225_09320"
     CDS             <1..1000
                     /locus_tag="VF225_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7145491.1"
                     /translation="GLWLLIREASAAKNLRALAPLVTEFGPSRMAFCTDDREPEHIAE
                     DGHVNSMVRDAVAAGIAPEDALVLATLNPATWHGLSHLGAVAPGYQADLLLLPDLERF
                     EPELTLKAGSPVEEIPEPDVPEWVKHSVRNRPVSSADFEIPWEGGQARAIGLVPDQVV
                     TASVEVEPATVNGFAVADAGNDLAKIAVIERHLGTGRIGHGFVHGSGLQRGALASTVS
                     HDAHNIIVVGMDDEDMTRAVERLGELGGGIVVVDGGEVKAELPLPVAGLLADAPLAEV
                     IEKSLACNDAAREVGWSGATPFLTLSFLGLSVIPSLKITDRGLVDVDRFQIVPLKI"
     gene            997..2319
                     /locus_tag="VF225_09325"
     CDS             997..2319
                     /locus_tag="VF225_09325"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX7145492.1"
                     /translation="MSTLLANGHVVTMDDAGTEYEGGWILVDGGLVKDAGAGAKPDAD
                     QVQDLGGAVVTPGLVNTHHHLWQNLTRARAQDENLFGWLKALYPVWAAIDEEAEYAAA
                     RAGLAELALSGCTTVFDHHYLFPPGVTGLLEAEIRAACELGVRIVAARGSMDLGESHG
                     GLPPDSVVESRDDALAATDAAAALADGARVEIAVAPCSPFSVTRELMTESAGLARKLG
                     LRLHTHLAETVEEEDYCRELYGCTPVEYLGEVGWLAEDVWCAHCVHLSDEDVETFGVQ
                     DMGVAHCPTSNMRLGAGIARVRELLDANVRVGLGVDGSASNERGDLFLEVKQALLVAR
                     ARGGPKAMTAREALRLGTRGGAAVLGRSDIGELSPGKRADIAVWRTDGLELGGAGDLV
                     AGLVFSGPHRVDRLYVNGEEVVRDGHLVRADESEIAKEHRVQAERFAS"
     gene            2316..2606
                     /locus_tag="VF225_09330"
     CDS             2316..2606
                     /locus_tag="VF225_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX7145493.1"
                     /translation="MSISLSTHVLDIERGLPATGVRVELWREDLVTASDTDADGRIAP
                     LATDLEPGVYRLLFRPPSPFFKGISVEVELEDGHYHIPLLVSSYGCTTYRGS"
     gene            2582..3025
                     /locus_tag="VF225_09335"
     CDS             2582..3025
                     /locus_tag="VF225_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX7145494.1"
                     /translation="MHDLPRQLTVEQLAELFEGRTRLVEKLAERDDPLASTHEVIAHL
                     SQAEKLEALAAHPAIGAKNLSVRSAAEQGGDDDPETLAELERLNAEYEERFGFRFVVF
                     VNRRSKSEIVPILRERLGCSREQELDTAVQELVAIAEDRWRRSSH"
     gene            3004..4206
                     /locus_tag="VF225_09340"
     CDS             3004..4206
                     /locus_tag="VF225_09340"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX7145495.1"
                     /translation="MASLLALDPYVTDWLNLLTRWLHVVAGIVWIGSSFYFIALDNHL
                     RPPKDEREREAGIGGETWEIHGGGFYNIKKYLVAPRVLPETLHWFKWEAYTTWLSGFA
                     LMIVLYYLNADTYLIDKSVADLTTGEAVAISIGLLAAAWVVYDILCRLLAAKPFVLAA
                     VLLGLITLAAWGVSHLFSGRATYLQIGAMLGTMMVGNVFFVIIPAHWELVRAKQAGRE
                     PDPAANARGKLRSVHNNYLTLPVVFTMLSNHFPFTYGHSYSWLILVALLVIGAWVRHF
                     FNLRHMGRTVWAIPVTAAIAIAVLAILIRPQSESVAGTAPVPFAQVARIVDQRCTACH
                     SQHPTKVDAAPRGIKLDTPEEIKTQASAIEQQAVQTYAMPLGNVTGMTQSERDLLGRW
                     IAQGARIP"
     gene            4213..4623
                     /locus_tag="VF225_09345"
     CDS             4213..4623
                     /locus_tag="VF225_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX7145496.1"
                     /translation="MLEITSGELRFLARFEEELAPQTVAAFRRILPLDDRIIHCRWSG
                     ESNWIPWGDRELGIGPENATSYPHPGELALYPGGQSETELLLPYGYCSFASKAGPLWA
                     NHFATIVEGKEQLEELGRRTLWEGAQPISFREVG"
     gene            4620..5816
                     /locus_tag="VF225_09350"
     CDS             4620..5816
                     /locus_tag="VF225_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LanC-like protein"
                     /protein_id="HEX7145497.1"
                     /translation="MILYRPEAFEPLVDTLWSELRARAAIRDIVEDADSAFRGPRLFW
                     RANDWDGWHSTSPMKNLYVGTAGVLWALDRLRRRGHTETRLDLAGLATRNVELFRERP
                     DFMKGEKVPAQRESALLTGEAGSLLVAWRLSPSRELADDLLARVQANVENEAEEVMWG
                     SPGTLLAAEAMFEWTADERWRDAWNESADALLARRDEEGYWTQRLYGQELRGLTPPHG
                     LVGNVQSLLPLLDENRAAALKRETAALLEANAVQADGLANWPPRPRPELPGPDGQIRV
                     QWCAGAPGIVIGAATYLDEKLLRAGAELPWRTGPPNLEKGPGICHGTAGNGYAFLKAF
                     ARTGDELWLERARRFAMHALAQVERLRNEHGRGRHSLWTGDVGAALYAAECIDGTSRY
                     PIFDGF"
     gene            complement(5820..6779)
                     /locus_tag="VF225_09355"
     CDS             complement(5820..6779)
                     /locus_tag="VF225_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145498.1"
                     /translation="MNNSLVVVVLASGVLYGTPLLYAALGELLAERSGVLNLGVEGMM
                     LVGAVMGFWAVQRVPGGSALVLTAGIGVAALAGAAMAAIHAFLVVTLRANQIVSGLAL
                     TIFAGAAGLSSYLGNDLKLTGSPARHQFQEVFPKSFQDLPVIGPMVFGQSILVYASWV
                     CVILVALYLTRTRTGLNVRAVGESPAAADAMGVNVAAYRYAHTLAGGAFAGVGGAAFS
                     LSITPQWADGLTGGAGWIAIALVIFAFWRPTLCLVGAYFFGAFTALPFALQGHGVTVA
                     PELFQTLPYVATILALVLVSSRAARLRFGAPAALGLPYVREER"
     gene            complement(6776..7894)
                     /locus_tag="VF225_09360"
     CDS             complement(6776..7894)
                     /locus_tag="VF225_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145499.1"
                     /translation="MRIERRLRQPWWLTVVVPVASIVVAFVLSAGVLLLTGHPPIHSF
                     HRLFDAAFIAHGALSDTVVSATPLAFTGLAAAVAFRMNLFNIGGEGQLYAGAITGATV
                     ALLMAGAPSPLVIAGMIVAGAVGGALLAAIPAVLRAFFSTNEIITSLMLNYVVGLVLE
                     YLIFDSHSYLRDTSGFEASVFPQGKMLPDEATWSSLRVHSLLLPLGFLVAIGVAVLVW
                     VLYARTRFGFEVQVIADSPRAGRYAGMRTRRKILAVMCLSGAIAGIGGASQDGDFRHQ
                     VDPRGLRQASYGYTGIVVAALARYNPLAVVPVAVLLGGLQNAGFALQGVDFPNGLVGV
                     MQGLILFCVLGGELFVRYRVRFERGPRAAKSPAPEPAP"
     gene            complement(7894..9459)
                     /locus_tag="VF225_09365"
     CDS             complement(7894..9459)
                     /locus_tag="VF225_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145500.1"
                     /translation="MGGEVQASPPVSPTMPAAVALRGITKRFPAVVANDHVDFEAAEG
                     EVHALLGENGAGKTTLSNILTGLYRPDEGEILLAGRPVDFHSPRDAIAAGIAMVHQHF
                     RLVSPFTVAENVVLGDQRDIGRSFFMHTRAIEARVEELSLRYGLAVDARARIWQLSVG
                     EQQRVEVLKALYQEARILILDEPTAVLTPQEADALFETVRAMAADGRTVIFISHKLHE
                     VKAVADRVTVLRRGRSIATVPASEATPRSLAALMVGREVEIGRRHHDRPAAGDAVLEL
                     DGVWADGDRGVPAVKDVSLTVRGGEIVAVAGVAGNGQRELAETIVGMRPATKGTVEIN
                     GRRVRSGDPRSAISAGAAYVPEDRLGTGVAPGLSIASNVVLKSYRRSPVSSGPFLRFA
                     RIRDLANRAIDKYDVRTSGCQVPAWQLSGGNVQKVVLALEISGEPDVLIAASPTRGLD
                     VSSIETVHSYLREAASTGVGVLLISEDLDEILALADRIAVMYEGGVAGERDAESATVE
                     ELGLLMAGGQDQD"
     gene            complement(9464..10570)
                     /locus_tag="VF225_09370"
     CDS             complement(9464..10570)
                     /locus_tag="VF225_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7145501.1"
                     /translation="MTKRKFALALPFALAAALILALGVSTGSARTQKKAASLKAAWMY
                     VGPHNDNGWSQAHDQGRLYVQKKLGSEVETTYKELVPEGPQTTQVIESLIRDGNKIIF
                     ATSFGFQPAMVAAAKKYPDVKFEMATGTAQSANMAEYFGAGEDGIYLSGMAAGAATKK
                     GVIGYVVPFAIPEIIRHANAFALGAQATHPGAKVRLIWTNAWLDQGKEKKAAQSLVSA
                     GADVLGQNVDSPAAGQYAESKGIPWVGYDSDARKFAPKQWLTAAVYNWGPYYLRKTKA
                     LLNGTWKTNFYYGSLKDGMIGLASYGPKVSAKTKAAIAKKKQALIKGTFYEFAGPLYD
                     QKGKLRVPKGKRLTVKDLYAMNWLVKGVIGSAKG"
     gene            complement(10633..11634)
                     /locus_tag="VF225_09375"
     CDS             complement(10633..11634)
                     /locus_tag="VF225_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX7145502.1"
                     /translation="MRVHERLDELYAIGGGVGADRVGGTPGEDRAHDLAAQWMQEAGL
                     EVEVDEAGNLIGRTAQSSVWTGSHLDSVPFGGKFDGALGVVAAIEAVERVGAGAVVVF
                     RDEERGCIGSSAFVERGDLPECFLEVHIEQGPVLERAGAPLGLAMGIVGIVRGERTFE
                     GHVGHAGTVPMNDRVDALVAAAEYVLQVRDIATAVDGAVATVGVIDVEPGAVNVIPGR
                     VRVSVDARAPDRERLDRLVAELGLHADFRLEPVPMAEQPTGAFRAELQERGLPVVELP
                     SGAGHDAATLAGAGVPTAMLFVRSLHGGISHSPQEESSPADVELAVEVLAGALARLT"
     gene            complement(11842..13314)
                     /locus_tag="VF225_09380"
     CDS             complement(11842..13314)
                     /locus_tag="VF225_09380"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7145503.1"
                     /translation="MAVATEREYGLFINGELVEPASGDVRELTEPATGEPLGRAAMGG
                     EADVDRAVDAARAAVDGPWGKTPPTERSRLLHALADAIVANRKELAELEVHNVGKAIS
                     SVKAEVSQAVENFRFYASAIASIAGRSNPIGGSLLFYSLKEPVGVAGQIVPWNYPLMM
                     ATWKLAPALAAGCSVVLKPDEATPLTALRLGELAAEVGFPPGSINIVPGPGKTTGAYL
                     VRHPGVDKVAFTGSTQTGGEIMRLASAPIKRITLELGGKSPSLVFADANLDDAIPSSV
                     WSIYYSAGQSCEARSRVLVERSIYDDFVARFSAFAEQLRVGDPLDPETQVGSLISTAH
                     RDKVHGFVEQGREEGAKVVTGGAIGDGKGAFYPPTVLAGVESPMSVAQEEIFGPVVTL
                     IPFEDEKDAIRIANDVRYGLMATVWTGDPARGHRVASRIKAGTVGINMPYTAFPGIPF
                     GGYKQSGFGRELGLETLELYLETKSVLVSTSPKPINPFGL"
     gene            complement(13333..14430)
                     /locus_tag="VF225_09385"
     CDS             complement(13333..14430)
                     /locus_tag="VF225_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7145504.1"
                     /translation="MSTALAHAPSADATRLLYERHSSRIFGFCLSRLGSREEAEDAVQ
                     TTFLNAQRGLGRGVVPDYELAWLFKIAQNVCHNRHQSAQRRGRVEATQDLDALQDVLA
                     TPERGNAVSLADLTRALSAVPERQRRALLLREFQGLSYEEIGVELGVSVAAVETLIFR
                     ARRSVAAELGRVGTKQSRRGAVASILELFGWFFRGGAAPVKLAVAAATVATTATLAVA
                     PAFQEHTTPARPAPTPGAPTPAAPRPATATKLAPVVKLPQKTVRTHVGRPSTPTSSTV
                     APATSAPAVHTGSTPIRPEPSPPAPPAASTGPTTAPVLPLPLQVSVPDVVSPPALTVP
                     SVDTGLSLPPVTLPDVEVPQLPLPPLPPKLP"
     gene            complement(14521..15741)
                     /locus_tag="VF225_09390"
     CDS             complement(14521..15741)
                     /locus_tag="VF225_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7145505.1"
                     /translation="MSGVASTKPVPTLEGLSELARLSLDGSSEPIERILSLARAALDM
                     DVALLGAFDGDFVVEAMDGDNEWFDLEIGTRLPVEQTYCKRMTQGDLPHLIHDAAADE
                     RTADLPLTRESGIGAYIGAPIRLWDGTLYGTLCCLSRSAEPSLNDRDVRFLRVLAEIV
                     ADQVDREQLEGEKRKLEWSRIRAILDSDHMDIEFQPIFDLLDCRIVSLEALARFWTEP
                     MRPPSAWFAEATEVGLGVELELAAMRAALLRLDEFPADVAIALNVSPATALDTRFCEL
                     LLDVAERVVIEITEHAQVDDYDALRAALAPLRERGAQLAIDDVGAGFANLRHILRLAP
                     DIVKLDLSLTQEIARDPAREALASSLVGFAGGVGASIVAEGISSDEDLALLRTLGVDY
                     GQGFHLARPSSLLH"
     gene            complement(15738..16187)
                     /locus_tag="VF225_09395"
     CDS             complement(15738..16187)
                     /locus_tag="VF225_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145506.1"
                     /translation="MRHLHSSEVDRKEVVRVLVVDDHALFAEALMLTLGIDERIEVIG
                     SASSGVEAVSLAEALRPDVVLMDVHMPSMDGIEATRRVRRVSPASRVVMVTAARSPQI
                     AFHALAAGAERCIGKDTPALKLIDAILDAPRTATVTRLVPRQASIAS"
     gene            complement(16239..17600)
                     /locus_tag="VF225_09400"
     CDS             complement(16239..17600)
                     /locus_tag="VF225_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145507.1"
                     /translation="MEPRPQSSRLTWLAVALGAGACALLGSIGADARWLAALGGTILE
                     RGSIPSGVPYAAAPSVDWVNVPVLGELVFHALQVMGGDRGFLVAQLIAVTAALTLLAL
                     GMRALGASDAASAVVLMLVFFAAVPSFLIVRAQLFSLVLFCAALLLLRAESRRSSRRV
                     WLLVPLIVLWANLHGGVLLGLAIAAAYLVLERARQEPVVAASVLVACCAALFVTPALL
                     GSGHYYLGVLGSEAAQRGEGLWAPLSIGKPFDVLFIVLAIPLLVFAFRSGARVWELAC
                     IAAFAGLTLHAGRNSVWLLCFIAAPAAHGLGKRYLRDFSVSPRTLVLCAWVPAAFLVL
                     GLLQTPQQYGAGARVRSEAVRLAAGKPILADGMDAEQLALDGRRVWIANPLDAFSRRD
                     QRLYLDWLSANPAGDALLRDATAVVVARGSKPEQRLDRDRSFRRAALDARAAIYVRVP
                     SNE"
     gene            complement(17643..18806)
                     /locus_tag="VF225_09405"
     CDS             complement(17643..18806)
                     /locus_tag="VF225_09405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7145508.1"
                     /translation="MSRAVILSGVRTPIGRKDGALAALRPDDLAATVIAEAVARAGVP
                     PEQIEDVYFGAANQAGEDNRNVARMGVLLAGLPETVAGITVNRLCASGLSAVVSACHA
                     VAAGDGDLFVAGGVESMSRAPLVTAKPEYGDGTTYDTQLGWRFPNPRLEKMFPLESMG
                     ETGENVAERWNVSREEQDAFALRSQQRWAAAAQAGRFDDELVGVSGVARDEHPRPDTS
                     AEKLAALKPAFREGGTVTAGNASGINDGAAALVIASEEKAKELGVEPLGRFVASAVAG
                     VDPRVMGIGPVPAVRKLLARAGVSVDELDLVELNEAFASQSIAVIRELGLDEERVNVN
                     GGAIAIGHPLGMSGARLVVSLLHELRRRRGRYGLATLCVGVGQGQAALFERVS"
     gene            complement(18803..19630)
                     /locus_tag="VF225_09410"
     CDS             complement(18803..19630)
                     /locus_tag="VF225_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX7145509.1"
                     /translation="MLIRIGHSADPDDAFMAWGLVSGRVDSRGFEFEFVPEDIQVLNE
                     WALEGRLEVTALSLATYPLVQNRYALLPHGASIGSGYGPIVVAREELSPRDLRDVEIA
                     VPGRLTTAFLVLGMALGGPFAHRALAFDRILDEVKAGHAVAGLLIHEGQLTYADEGLV
                     KVLDLGEWWLLETGLPLPLGVNVARRDVPRLDELSVALRESIEAGLANRAEATQYALG
                     FARDLDAQTADRFIEMYVNELTCDYGDEGRQAVAELLRRAEATGVYEEPVRIEFVGT"
     gene            19657..20142
                     /locus_tag="VF225_09415"
     CDS             19657..20142
                     /locus_tag="VF225_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7145510.1"
                     /translation="MAKPALTDAIQDYLREIYKLGADGGRVSVTALAKRQGVSPASAS
                     AMVKKLAALELAVHQPYRGITLTQDGEKVALEVIRHHRLLELYLAETLGVDVNEVHGE
                     AERLEHALSEELEERIDKALGFPTHDPHGDPIPDANLEWPSQPTASAVGSGQRAKRSS
                     S"
     gene            complement(20153..20497)
                     /locus_tag="VF225_09420"
     CDS             complement(20153..20497)
                     /locus_tag="VF225_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145511.1"
                     /translation="MRRKTTEGRFAERTVHGVDDAGATETIVIWIERKQGALWGVGRA
                     VNPENRASDEPRRDDYLFEGYELDDALDAANGALEDDLTVSESDGRSENLKPFTRGEL
                     LKPLERWFFGRR"
     gene            20521..20784
                     /locus_tag="VF225_09425"
     CDS             20521..20784
                     /locus_tag="VF225_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145512.1"
                     /translation="MQRFASLLRSPFSFLFARSSTEERVAAYVIREHGRGRGLAEILE
                     DRYVQNRLSSQQRLRLLDRPELIQALGDETVEDTRRDLESARS"
     gene            complement(20781..21710)
                     /locus_tag="VF225_09430"
     CDS             complement(20781..21710)
                     /locus_tag="VF225_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7145513.1"
                     /translation="MTLFLSESDVEELLTPEAAVDAVERAFLRMAAGSVEIAPRRRLK
                     LQEGRLADMAASDLELGYACAKVYAGFAEGAAFVVALFAVDRPELVALIEADHLGRLR
                     TGAASAVGAKHLAREGASSLGVIGCGTQAETQVKCIRAAVPAIERVVVYCRTERNLLA
                     FCKRVDAEPAESNREAAEQDVVVTITTSRDPVLRGEWLRPGALVCAAGANNPAARELD
                     NVVLERASFVCCDWKEQARIESADLIEPIQQGVLDWLEVHQLHEVVAGELPGRQSPDD
                     IVVFKSNGIASWDVAVAAAVVERARQRGVGRKL"
     gene            complement(21707..21967)
                     /locus_tag="VF225_09435"
     CDS             complement(21707..21967)
                     /locus_tag="VF225_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX7145514.1"
                     /translation="MRTFSSFLGRMVETESGRKLGACRDLRASLGGGRPEVEAVVVGG
                     RGWLEHLGILRPSARPPKAVPWEAVVRIEGDRIVVRDGTELE"
     gene            complement(21964..22314)
                     /locus_tag="VF225_09440"
     CDS             complement(21964..22314)
                     /locus_tag="VF225_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145515.1"
                     /translation="MSKTIVLGLELVDRQLLDSEDRRCGNVDDLALEGRPGEKLEVVA
                     IFSGPGAWRRRAGLTGRFAAWIGGGRVVRIPWEEVAVVSAHVKLRKRADELGLGRGDD
                     RLRPLIQKIPGADR"
     gene            complement(22311..23534)
                     /locus_tag="VF225_09445"
     CDS             complement(22311..23534)
                     /locus_tag="VF225_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7145516.1"
                     /translation="MKIFQIALGILAAIGGFVDIGDLVFNVSAGATFGYQLLWVIVIG
                     VVGIIVYSEMCGRVATVSGKAVFDAVRERLGFRTGLAALVSAQVVNLMTLTAEIGGVA
                     ICLQLLSGLPYRWLIVIAGVVLSIVVWTTSFDWIERIFGYGGLCLLVFAVAAVKLHPA
                     WGAVGHGFVPHVPTQSPLLYAYFAVGLLGAAMTPYEVYFYSSGAVEEQWGPKDLGLNK
                     ANAMIGYGLGGFLSFALMIVGGALFLGKGIEPEFLGSIALGAEVPLGQIGLLLALVGI
                     LFAVGGAAIDTLFSGAYNLAQFLGWEWGRYRSNSGAPRFTLTWIVLLVLGLGLVMTGV
                     DPVQLTEYAVIFSAVALPLTYIPILLVANDRSYMREYVNGRLANVLGLFYLVVIVVIA
                     LTAVPLLILTNAGQN"
     gene            complement(23531..24004)
                     /locus_tag="VF225_09450"
     CDS             complement(23531..24004)
                     /locus_tag="VF225_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145517.1"
                     /translation="MIEGEPVTLVQDGRSTRVSSAKLIARVASALLVFALAGCNGGTV
                     DKHALKRDAEKVGSLATEGELMANDVSKGASTEYFVRVHAKELSGAAADFEDSLAKRP
                     TSPGIRSDVRKLSKLAGKVSRELEQLHLHPTDRDIARSLRQPLMDDADAADKLAK"
     gene            24068..24140
                     /locus_tag="VF225_09455"
     tRNA            24068..24140
                     /locus_tag="VF225_09455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24101..24103,aa:Arg,seq:gcg)
     gene            24266..24700
                     /locus_tag="VF225_09460"
     CDS             24266..24700
                     /locus_tag="VF225_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7145518.1"
                     /translation="MSPAGNKDLIRRYIQAIDDNRTSDWSILDEYIAEDFVAHNPPIP
                     GVSLDREGMKQAAEIFRVATPGRHEIGMQVEEDDLVVSQILGRGVHAGELLGIPATNR
                     EVETEGIVIHRVRDGKIVEYWSVTDVVRVLQQVGALPGPPPG"
BASE COUNT         4259 a         8425 c         8286 g         3764 t
ORIGIN      
        1 ggggctgtgg ctgctgatcc gcgaggcgtc ggcggccaag aacctgcgag cgctggcgcc
       61 gctcgtgacc gagttcgggc cctcccggat ggcgttctgc accgacgacc gcgagccgga
      121 gcacatcgcc gaggacgggc acgtcaactc gatggtgcgc gacgcggtcg cggccgggat
      181 cgcgcccgag gacgcgctcg tgctcgcgac gctgaacccg gcgacctggc acggcctctc
      241 gcatctcggg gccgtcgcgc ccggctatca ggccgacctg ctgctcctgc ccgacctgga
      301 gcgattcgag cccgagctga cgctgaaggc cggaagtccg gtagaagaga tcccggagcc
      361 cgacgtcccc gagtgggtca agcactccgt gcgcaaccgg ccggtgagct cggcggactt
      421 cgagatcccc tgggaaggcg gtcaggcgcg tgcgattggg ctcgtgcccg accaggtggt
      481 gacggcgtcg gtcgaggtcg agccggcgac cgtgaacggc ttcgccgtcg ccgacgccgg
      541 caatgacctc gccaagatcg cggtgatcga gcgccatctc gggacgggcc gcatcggaca
      601 cggcttcgtc cacggctcgg ggctgcagcg gggcgcgctc gcgtcgaccg tttcgcacga
      661 cgcgcacaac atcatcgtcg tcggaatgga tgacgaggac atgacacgtg cggtggagcg
      721 gctcggcgag ctgggaggcg gcatcgtcgt cgtggacggc ggcgaggtga aggcagagct
      781 gccgcttccc gtcgccggct tgctggccga cgcaccgctc gctgaagtga tcgagaagag
      841 cctggcctgc aacgacgcgg cccgtgaggt cggctggtcg ggtgcgacgc ctttcctgac
      901 cttgtcgttc cttgggctgt cggtgatccc gagtctgaag atcaccgatc gaggtctcgt
      961 cgacgtcgac cgcttccaga tcgtgccgct gaagatttga gcacgctgct cgccaacggc
     1021 cacgtcgtga cgatggacga cgccggcacg gagtacgagg gcggttggat cctcgttgac
     1081 ggcggcctgg tgaaggacgc cggcgcaggg gccaagcccg atgccgacca ggtgcaggat
     1141 ctcggcgggg cggtcgtgac cccggggctc gtgaacacgc accaccacct gtggcagaac
     1201 ctcacgcggg cacgcgcaca ggacgaaaac ctcttcggct ggctcaaggc tctgtacccg
     1261 gtctgggctg cgatcgacga ggaggccgag tacgccgctg cgcgcgccgg cctcgccgag
     1321 ctcgccctct ccggctgcac gacggtgttc gaccaccact atctcttccc gcccggcgtc
     1381 accggcctgc tcgaggcgga gatccgcgcg gcgtgcgagc tcggtgtgcg catcgtcgcg
     1441 gcacgagggt cgatggatct cggcgagtcg catggaggct tgccgcccga ctcagtggtg
     1501 gagtcgcgcg acgatgcgct cgccgcgaca gacgcagcgg ccgcgctcgc cgacggtgcc
     1561 cgggtcgaaa tcgccgttgc tccctgctcg ccgttctccg tcacccggga gctgatgacg
     1621 gagtcggcgg ggctggcacg caaactcggc ttgcggctgc acacgcacct cgccgaaacg
     1681 gtcgaggagg aggattactg ccgcgagctc tacggctgca cgcccgtcga gtatctgggg
     1741 gaggttggtt ggctcgccga ggacgtgtgg tgcgcgcact gtgtgcatct ctccgacgaa
     1801 gacgtcgaga ctttcggtgt gcaggacatg ggcgtcgcgc actgccccac gtccaacatg
     1861 cggctcggag ctgggatcgc acgcgtgcgc gagctgctcg acgcgaacgt acgggtcggg
     1921 ctcggcgtcg acgggtcggc ctcgaacgag cgcggcgatc tcttcctgga ggtcaagcag
     1981 gcgctgctcg tcgcgcgcgc ccgtggcggg cccaaggcca tgactgcgcg cgaggcgttg
     2041 cggctcggca cgcggggcgg cgcggcggta ctcggtcgca gcgacatcgg cgagctctcg
     2101 cctggcaagc gcgccgacat cgccgtctgg cggacggatg gcctggagct cggcggcgcc
     2161 ggcgatctcg tcgccgggct tgttttctcg gggccccatc gagtggatcg cctctacgtg
     2221 aacggcgaag aggtcgtccg tgacggccac ctcgtgcgag ccgatgaaag cgagatcgcg
     2281 aaggaacatc gcgtccaggc ggaaagattc gcgtcatgag catctcgctt tccacccatg
     2341 tcctggacat cgagcgcggc ctgccggcga ccggcgtgcg cgtcgagctg tggcgcgaag
     2401 acctcgtcac agcgagcgac accgatgcgg acggccgcat cgcccctctc gccaccgatc
     2461 tcgagcccgg cgtctatcgc ctgctgttcc gacctccctc gccgttcttc aagggaatca
     2521 gcgtcgaggt cgagctcgag gacggccact accacatccc actgctcgtc tcctcctacg
     2581 ggtgcacgac ctaccgcggc agctgaccgt cgagcagttg gccgagctgt tcgaagggcg
     2641 cacgcgactc gtcgagaagc tcgccgagcg tgacgatccg cttgcgtcta cacacgaggt
     2701 gatcgcgcac ctgagccagg ccgagaagct cgaagcgctc gctgcacatc cggcgatcgg
     2761 cgcaaagaac ctctcggtgc gctccgctgc tgaacagggt ggagacgacg acccagagac
     2821 gctcgcggag ctcgagcgac tcaacgccga gtacgaggag cggttcggct ttcgtttcgt
     2881 cgtgttcgtg aaccgccgct cgaagagcga gatcgtcccg atcctgcgtg aacggctcgg
     2941 gtgctcgcgc gagcaggagc tcgacacggc cgttcaggag ctggtcgcga tcgcggagga
     3001 ccgatggcgt cgctcctcgc actagatccg tacgtcaccg actggctcaa cctgctcacg
     3061 cggtggttgc acgtcgtcgc cggcatcgtc tggatcggtt cctcgttcta cttcatcgcg
     3121 ctcgacaacc acctgcgtcc gcccaaggac gagcgggaac gagaagccgg tatcggcggc
     3181 gagacgtggg agatccacgg cggcggcttc tacaacatca agaagtatct cgtcgcgcca
     3241 cgcgtcctac cggagacatt gcactggttc aagtgggagg cgtacacgac gtggctgagc
     3301 ggcttcgcgc tgatgatcgt cctctactac ctcaacgcgg acacgtatct gatcgacaag
     3361 agcgttgccg acctcacgac aggtgaagct gttgcgatca gcatcggcct gttggcggcc
     3421 gcgtgggtcg tgtacgacat tctctgccgc ctgctcgccg cgaagccttt cgttcttgcg
     3481 gcggtcctgc tgggattgat cacactcgcc gcgtggggcg ttagccacct cttcagcgga
     3541 cgggccacgt atttgcagat cggcgcgatg ctcgggacga tgatggtcgg caacgtcttc
     3601 ttcgtgatca ttcccgcgca ctgggagctc gtgcgcgcga agcaggcggg gcgcgagccg
     3661 gacccggcgg cgaacgcccg cgggaagctg cggtccgttc acaacaacta cctgacgctg
     3721 cccgtcgtct tcacgatgct ctcgaaccac ttcccgttca cctacgggca cagctactcc
     3781 tggctgatcc tggtcgcgct gctcgtcatc ggcgcctggg tccgccactt cttcaatctg
     3841 cgccacatgg gacgaaccgt gtgggcgatt ccggtcaccg cggcgatcgc gatcgccgtg
     3901 ctcgcgatcc tgatccgccc gcagagcgag tctgtggctg ggacggcgcc cgtcccgttc
     3961 gcgcaggtcg cgcgcatcgt cgaccagcgc tgcaccgcct gccactcgca gcacccgacg
     4021 aaggtggacg cggcgccgcg ggggatcaag ctcgacacgc cggaggagat caagacgcag
     4081 gcgtccgcaa tcgagcagca ggccgtgcag acttacgcga tgccgctcgg gaacgtcacc
     4141 ggcatgacgc agagcgagcg tgacctcctc gggcgctgga tcgcccaagg agcgagaatc
     4201 ccctaagtgc cgatgctcga gatcacgagc ggcgagctcc gctttctcgc gcgcttcgag
     4261 gaggagctcg cgccgcagac cgtcgctgcg ttccggcgca tcctcccgct cgacgatcgc
     4321 atcatccact gccgctggag cggggagtcc aactggattc cctggggcga tcgcgagctg
     4381 ggaatcgggc cggagaacgc cacctcgtat ccgcaccccg gcgagctcgc tctctacccc
     4441 ggcggtcaaa gcgaaacaga gttgctcctt ccttacgggt actgctcctt cgcatcgaag
     4501 gcgggacctc tttgggcgaa ccatttcgcg acgatcgtcg aaggaaaaga gcagctcgag
     4561 gagctcggcc gacgcacgct ctgggagggg gcgcagccga tctcgtttcg ggaggtcggg
     4621 tgatcctgta ccggccggag gccttcgagc cactggtcga cacactctgg agcgagctac
     4681 gagctcgcgc agcgattcgc gacatcgtcg aggacgccga ctccgccttc cgagggccgc
     4741 gactcttctg gcgggcgaac gattgggacg gctggcactc gacgagcccg atgaagaacc
     4801 tgtacgtggg caccgccggc gtgctctggg cactcgatcg tctgcggcga cgcggccaca
     4861 ccgagaccag gctcgatctc gccggcctcg cgacccgaaa cgtcgagttg ttccgcgaac
     4921 gaccggactt catgaagggc gagaaggtcc cggcgcagcg agagtccgca ctgctcacag
     4981 gagaggccgg aagtctcctt gttgcctggc gcctctctcc gagccgcgag ctcgcggacg
     5041 atctgctcgc acgcgtacaa gccaacgtgg agaacgaggc ggaagaagtg atgtgggggt
     5101 cgccgggcac gctgctagcc gccgaggcga tgttcgagtg gaccgcagac gagcgctggc
     5161 gtgatgcgtg gaacgagagc gccgacgcgc tccttgcacg ccgagacgag gagggctact
     5221 ggacccagcg cctctacggc caggagttgc gcggcctcac gccgccgcac ggcctcgtcg
     5281 gaaacgtcca gtcgttgctt ccactactcg acgagaatcg cgccgccgcg ctgaagcgtg
     5341 agaccgctgc gctgctcgaa gcgaacgcgg tccaggcgga cgggttggcg aactggccgc
     5401 cacggccgcg tcccgagctg cccgggccgg acggccagat ccgcgtccag tggtgtgcgg
     5461 gagccccggg aatcgtcatc ggcgccgcga catatctcga cgagaagctc cttcgcgccg
     5521 gcgccgagtt gccgtggcgc acgggaccac cgaatctcga gaaggggccg ggcatctgtc
     5581 acggcaccgc cggcaacggt tacgcattcc tcaaggcctt cgcgcgaacc ggtgacgagc
     5641 tgtggctcga gcgcgcgcgc cgtttcgcca tgcatgcgct cgcgcaagtc gaacgattgc
     5701 gcaacgagca cggccgcggt cgccactcat tgtggaccgg ggacgttggc gcggcgttgt
     5761 acgcggctga gtgcatcgac ggcacgagcc gctatcccat cttcgacggc ttctaggcgc
     5821 taacgctctt cgcgcacgta cggtaagccg agcgccgcgg gcgcgccgaa ccgtaaccgc
     5881 gctgcccgtg aagacacgag cacgagggcc aggatcgtcg ccacatacgg cagcgtctgg
     5941 aagagctcag gcgcgaccgt cacaccgtga ccctgcagcg cgaacgggag cgccgtgaag
     6001 gcaccgaaga agtaggcgcc gacaaggcag agcgtcggcc tccagaaggc aaagatcacg
     6061 agcgcaatcg cgatccagcc ggcgccgccg gtcaggccgt cagcccactg cggcgtgatc
     6121 gagaggctga atgccgctcc gcccacgcct gcgaaagcgc cgccggcgag ggtgtgcgcg
     6181 tagcggtagg cggcgacatt gacccccatc gcgtcggccg cagcgggaga ctcgccgacc
     6241 gcgcgcacgt tgagaccggt acgcgtccgc gtgaggtaaa gcgcaacgag gatgacgcag
     6301 acccacgacg cgtacaccag gatcgactgc ccgaacacca tcggaccgat cacgggcaag
     6361 tcctgaaacg actttgggaa gacctcctgg aactgatgcc tcgccgggga gccggtgagc
     6421 ttcagatcgt tgccgaggta ggacgagagc cccgcggcgc cggcgaagat cgtcagcgcg
     6481 agacccgaga cgatctggtt cgcacgcaag gtcacgacga ggaacgcgtg gatcgcagcc
     6541 atcgcggccc cggccaacgc agctacgccg atccctgccg tgaggacgag cgccgaaccc
     6601 ccgggcaccc gctgcactgc ccagaagccc atgacggcgc cgacgagcat cattccctcg
     6661 acgccgaggt tgagcacgcc ggagcgttcg gccagcagtt cgccgagcgc tgcatagagg
     6721 aggggcgtcc cgtagagaac gcctgaggcc agcacgacga cgacgaggct gttgttcacg
     6781 gggccggctc cggcgccggt gatttggcgg ctcgtggccc acgttcgaag cgcacgcggt
     6841 agcggacgaa gagctcgccg ccgagaacgc agaacaggat caggccttgc atcacgccca
     6901 cgaggccgtt cgggaaatca acgccctgca aggcgaagcc cgcgttttgc aagccgccga
     6961 gcagcactgc aacgggcacc accgccaacg ggttgtagcg tgcaagtgct gcaacgacga
     7021 tgcccgtgta cccgtagctc gcctgacgaa ggccgcgcgg atcgacttga tggcgaaagt
     7081 caccgtcctg gctggcgcct ccgatccccg cgatcgcacc ggagaggcac atgacggcca
     7141 ggatcttccg gcgcgtccgc attcccgcat agcggcccgc gcgcggggag tccgcaatca
     7201 cctgcacctc gaagccgaag cgcgttcgtg cgtacaggac ccaaacgagc acggccactc
     7261 cgatcgccac gaggaagccg agtggcaata gcaggctgtg caccctcaag ctcgaccacg
     7321 tggcctcgtc gggcagcatc ttgccctgcg ggaagacgct cgcttcgaac ccgctcgtgt
     7381 cgcgcaagta ggagtggctg tcgaagatca gatactcgag gacgagaccg accacgtagt
     7441 tgagcatcag cgacgtgatg atctcgttcg tcgagaagaa cgcccgtagg accgcgggaa
     7501 ttgccgccag gagcgcgccc ccgacggccc cggcgacgat catgcctgcg atcacgaggg
     7561 gcgacggcgc gccggccatc agcagtgcaa cggtggcgcc cgtgatcgcc ccggcataga
     7621 gctggccctc accgccgatg ttgaagaggt tcatccggaa ggcgaccgcg gcagccaacc
     7681 ccgtgaaggc gagtggcgtc gccgagacga ccgtgtcgct gagcgccccg tgcgcgatga
     7741 acgccgcgtc gaacaggcgg tggaaggaat ggatcggagg gtggcccgtc agcaacaaaa
     7801 cgccggcgga aagcacgaag gcgaccacga tcgaggcgac cggaacgaca accgtcagcc
     7861 accagggctg tcggaggcga cgctcgatcc gcatcagtct tggtcctgac cgcctgccat
     7921 gagcaggccg agctcctcga cggtggccga ttccgcgtcg cgttcgcccg caacgccgcc
     7981 ctcgtacatc acggcgatcc tgtcggcgag ggcgagaatc tcgtcgaggt cctcgctgat
     8041 gagcaacacg cctaccccgg ttgaggcggc ctcgcgtagg taggagtgga cggtttcaat
     8101 gctggagacg tcgaggccgc gcgtcgggga ggctgcgatg agaacgtccg gttcaccgga
     8161 gatctcgagt gcaagcacga ccttttgcac gttcccgccg gagagctgcc aggccggcac
     8221 ctgacaccca gaggtgcgaa cgtcgtactt gtcgatcgcg cgattcgcga ggtcgcgaat
     8281 gcgcgcaaac cgcaagaaag gcccgctcga gaccggcgag cgacggtacg acttgaggac
     8341 aacgttggac gcgatgctca aaccaggcgc cacgccggtg cccagacggt cttcggggac
     8401 gtaagcggcg ccggccgaga tcgcgctgcg cggatcgccg cttcgcaccc gacgtccgtt
     8461 gatctcgacc gttcccttgg tcgcaggccg catgccgaca atcgtctcgg cgagctcgcg
     8521 ctgaccgttc cccgccacgc ccgcgaccgc gacgatctca ccgccacgca cggtgagcga
     8581 gacgtccttg acggccggta cgccgcggtc gccgtctgcc cagacgccgt cgagctccag
     8641 cacggcgtca cccgcggcag ggcgatcgtg gtgacgccgc ccgatttcga cctcgcgtcc
     8701 gaccatcaag gcagcaaggg atcgcggtgt cgcttccgag gcggggaccg tcgcgatcga
     8761 gcgcccgcgg cgcaacactg tgactcggtc cgcgacggcc ttcacttcat ggagcttgtg
     8821 cgagatgaag atcacagtgc gtccgtcagc tgccatcgcg cgcactgtct cgaagagtgc
     8881 gtcagcttcc tgcggggtca gcacggcggt cggctcgtca aggatgagga tgcgggcctc
     8941 ctggtaaagc gccttcagga cctcgacgcg ctgttgttca ccgaccgaga gctgccagat
     9001 gcgggcgcgc gcgtcgacgg cgagcccgta ccgaagggac agctcctcga ctcgagcttc
     9061 gatcgcgcgc gtatgcatga agaacgagcg gccgatgtcc cgctggtcgc cgaggacgac
     9121 gttctcggcg acagtgaagg gcgagacgag gcggaagtgc tggtggacca tcgcgatccc
     9181 tgctgcgatc gcgtcgcggg gcgagtggaa atccaccggc cggccggcga gaaggatctc
     9241 gccttcgtcc ggtcggtaaa gaccggtgag gatgttggag agcgtcgtct tgccggcgcc
     9301 gttctcaccc agaagggcgt gcacctcgcc ctccgcggcc tcgaagtcga cgtggtcgtt
     9361 cgcaacgacc gcgggaaacc gctttgtgat tccccgcaag gcaacggcgg ccggcatggt
     9421 gggagagacg ggcggggaag cctggacttc cccgcccatg gaatcagccc ttggcgctgc
     9481 cgatcacgcc tttgacgagc cagttcatcg catagaggtc cttgaccgtc aagcgcttcc
     9541 ccttcggaac acgcagcttg cccttctggt cgtagagcgg acccgcgaac tcgtagaacg
     9601 tgcccttgat cagagcctgc ttcttcttcg cgattgcagc cttcgtcttg gcgctgacct
     9661 tcggcccgta agaagcgagg ccgatcatgc cgtccttgag gctgccgtag tagaagttcg
     9721 tcttccaggt cccattcagc aaggccttgg tcttgcgtag gtagtagggg ccccagttgt
     9781 agaccgcagc ggtcagccac tgcttcggag cgaacttgcg agcgtctgag tcgtagccca
     9841 cccacggaat gcctttcgac tccgcgtatt gaccggccgc cggactgtcg acgttctggc
     9901 ccaggacgtc cgcgccggcc gagacgaggc tctgggctgc tttcttctcc ttgccctgat
     9961 cgagccaggc gttcgtccag atcagtctga cctttgcgcc gggatgggtt gcctgcgcac
    10021 cgagcgcgaa cgcattggcg tgccggatga tctccggaat cgcaaacgga acgacgtagc
    10081 cgatgacacc cttcttcgtt gcggcacctg ctgccatccc ggagaggtag atcccgtcct
    10141 ctcctgcccc gaagtactcc gccatgttcg cggactgcgc agtgcctgta gccatctcga
    10201 acttcacgtc ggggtacttc ttcgccgccg ccaccatcgc gggctgaaaa ccgaaagagg
    10261 tagcgaagat gatcttgttg ccgtctcgaa tgaggctctc gatgacctgt gtggtctgcg
    10321 gcccttccgg cacgagctcc ttgtaggtcg tctcgacctc ggagccgagc ttcttctgca
    10381 cgtacaagcg gccctggtcg tgtgcctgcg accagccgtt gtcgttgtgc ggaccgacgt
    10441 acatccaggc ggccttgagg gacgccgctt tcttctgcgt cctcgccgag ccggtcgaga
    10501 cgccgagcgc gaggatcagc gcagcggcga gcgcgaacgg cagggcgaga gcgaacttcc
    10561 gtttcgtcat gttctcctct ctcctgtccc gggggtgccg ttcgcgacta tatggcggtc
    10621 ggctcgcggt gcctaggtca gccgtgcgag agccccggcg aggacctcga cggcgagctc
    10681 gacgtcggcc ggggaggact cctcctgcgg cgaatggctg attccgccgt gcaggctgcg
    10741 gacgaagagc atcgccgtcg ggacgcctgc gccggcgagg gtggcggcgt cgtgaccggc
    10801 tcccgagggg agctcgacga cgggcaggcc ccgctcctgg agctccgcgc ggaaggcccc
    10861 ggtcggctgc tcggccatcg ggaccggctc gaggcggaag tccgcgtgga gcccgagctc
    10921 cgcgacgagg cggtcgaggc gctcccggtc gggggcgcgg gcatcgaccg agacgcgcac
    10981 ccggccgggg atcacgttga ccgctccggg ctcgacgtcg atcacgccca ccgtggccac
    11041 cgccccgtcc acggcggttg cgatgtcacg cacctgcagc acgtactcgg ctgcggccac
    11101 cagcgcatcc acgcggtcgt tcatcggaac ggtgcccgcg tgcccgacgt gcccctcgaa
    11161 ggtgcgctcg ccgcgaacga tgccgacgat ccccatcgca agtcccagcg gggcgcccgc
    11221 ccgctccagc acgggaccct gctcgatgtg cacttcgagg aagcactccg gcaggtcgcc
    11281 gcgctcgacg aaggcggagc tgccgatgca gccgcgctcc tcgtcgcgga agacgacgac
    11341 cgcgcccgcg ccgacacgct cgaccgcctc gatcgcggcg acgaccccga gcgcaccatc
    11401 gaacttccct ccgaacggca ccgagtcgag gtgcgagccg gtccagacgc tcgactgcgc
    11461 cgtgcggccg atgagattcc ccgcctcgtc gacctcgacc tcgagcccgg cctcctgcat
    11521 ccattgggcg gccaagtcgt gcgcccggtc ttcgcctggg gtgccgccaa cacggtccgc
    11581 gccgacaccg ccgccgatgg cgtagagctc gtcgagccgc tcgtgaacgc gcacgtggcg
    11641 atcatccctc ctgggcgtga cgccatcccg ggcgtaccgg tgccaggcac gggtacgact
    11701 ctgtgacagc ttcgcgtcga gagcccgaag ggtcgaccgg cttccggctc ctcgagcgga
    11761 gcaggctctt cgtctcgggc gcgactccac aggacacgta ccggtgccag gcacgggtac
    11821 gactccggaa cgagtctgtg cctagagccc gaacgggttg atcggcttcg ggctcgtcga
    11881 gacgagcacg ctcttcgtct cgagatagag ctcgagcgtc tccaggccga gctcgcgacc
    11941 gaagccggac tgcttgtagc ccccgaacgg gatccccggg aaggccgtgt acggcatgtt
    12001 gatcccgacg gtgccggcct tgatgcggct tgcgacgcgg tggccgcgcg cgggatcgcc
    12061 cgtccacacg gtcgccatca gcccgtagcg cacgtcgttc gcgatgcgga tcgcatcctt
    12121 ctcgtcctcg aacgggatca gtgtcacgac gggcccgaag atctcctcct gcgcgaccga
    12181 catcgggctc tcgactcccg cgagcacggt cgggggatag aaagcaccct tgccgtcgcc
    12241 gatcgctccc ccggtgacca ccttcgcgcc ctcctcgcgt ccctgctcga cgaacccgtg
    12301 caccttgtcg cgatgcgccg tggagatgag ggagccgacc tgcgtctccg gatcgagcgg
    12361 atcgccgacg cgcagctgtt ccgcgaacgc ggagaacctg gcgacaaagt cgtcgtagat
    12421 cgaccgctcg acgagcacac gcgagcgcgc ctcgcagctc tgcccggcgg agtagtagat
    12481 cgaccacacc gagctcggga tcgcatcgtc gagattcgcg tcggcgaaga cgaggctcgg
    12541 gctcttgccg ccgagctcga gcgtgatccg cttgatcggc gccgacgcga gccgcatgat
    12601 ctcgccgccc gtctgcgtgg agccggtgaa cgcaaccttg tcgacgcctg gatgacgaac
    12661 gagatatgcg cccgtcgtct tgccgggccc ggggacgata ttgatcgagc ccggcggaaa
    12721 cccgacctcc gccgcgagct ccccgagccg cagcgcagtc aggggcgtcg cctcgtcggg
    12781 cttcagcacg acggagcagc ccgcagccag agcaggcgcg agcttccagg tcgccatcat
    12841 cagggggtag ttccacggga cgatctggcc cgcgacgccg acgggctcct tcagcgaata
    12901 gaacaacagc gagccgccga tcggattcga ccgccccgcg atcgacgcga ttgcagacgc
    12961 gtaaaagcgg aaattctcga ccgcctggct cacctccgcc ttgacggacg aaatcgcctt
    13021 gccgacgttg tgcacctcga gctcagcgag ctccttgcgg ttcgcgacga tcgcgtcggc
    13081 gagcgcatgc agcaagcgtg agcgctcggt tggcggcgtt ttaccccacg gtccgtcgac
    13141 ggccgcgcgt gccgcgtcca ccgctcggtc gacgtcggcc tcaccgccca tcgcggcacg
    13201 tcccagcggc tcacccgtcg caggctccgt cagctcacgg acgtcccccg acgccggctc
    13261 gacgagctcg ccgttgatga agagaccgta ttcgcgctcg gtcgcgaccg ccatcgctgg
    13321 cgaggatact gactagggca gcttcggcgg cagcggggga agcgggagct gcggcacctc
    13381 aacgtccggc aaggtgaccg gcggcaggga cagcccggtg tcgacactcg ggaccgtcag
    13441 ggcaggcggg ctcaccacgt ccggcacgga cacctgcagc gggagcggaa gcaccggcgc
    13501 tgtcgtcgga ccggtgctcg ctgccggcgg agcgggcggc gacggctcgg ggcgaatcgg
    13561 agtcgagccg gtatggaccg cgggcgccga cgtcgccgga gcgacggtcg agctcgtggg
    13621 cgtggagggc cgacctacgt gcgtgcgcac cgtcttctgc ggaagcttga ctaccggcgc
    13681 gagcttcgtc gcggtcgcag gcctcggggc agcaggcgtc ggcgcaccag gcgtcggcgc
    13741 agggcgcgcc ggcgtcgtgt gctcctggaa tgcgggcgca acagcgagcg tcgcggtcgt
    13801 cgcaacggtc gcagcagcaa ccgcgagctt gaccggcgcg gcgccacccc tgaagaacca
    13861 gccgaagagc tccaggatcg aggcgacggc gccgcggcgc gactgcttcg tcccgacacg
    13921 cccgagctct gcggccaccg aacggcgcgc acggaagatc agcgtctcga cagccgcgac
    13981 ggaaacgccg agctccacgc cgatctcctc gtatgacagc ccctgaaact cccgcaggag
    14041 aagcgcccgc cgctgtcgct cgggcaccgc cgagagcgcg cgcgtcagat cggcaagcga
    14101 gacggcgttg ccgcgctccg gcgtcgcgag cacatcctgc agcgcgtcca gatcttgcgt
    14161 cgcctcgacg cgtccacgtc gctgcgcgga ctgatggcgg ttgtggcaga cgttctgcgc
    14221 aatcttgaag agccaggcga gctcgtagtc cggaacgacg ccgcgcccga ggccgcgttg
    14281 cgcgttgagg aacgtcgtct gcaccgcgtc ctcggcttcc tcgcgcgagc cgagccggct
    14341 caggcagaag ccgaagatcc gcgacgagtg ccgctcgtag agcaggcggg tcgcgtcagc
    14401 agacggggcg tgggcgaggg cagtgctcaa gcacactcac tctggaatgg aaagggactt
    14461 ttggcaaggg gggtgccgtg ggcacctgcg gaaaggaggc gcccacggcc tgggggagga
    14521 tcagtggaga agggaggacg gcctggcgag gtggaagccc tggccgtagt cgacgccgag
    14581 agtgcgtagg agcgcgagat cctcgtcgct cgagatgccc tcggccacga tcgaggctcc
    14641 gacgcctccg gcaaatccga cgagtgacga cgcgagcgcc tcgcgagcgg ggtcgcgcgc
    14701 gatctcctgc gtcaggctca gatcgagctt cacgatgtcg ggcgcgagcc ggaggatgtg
    14761 gcggaggttc gcgaagccgg cgccgacgtc gtcgatcgcg agctgcgccc cgcgttcacg
    14821 cagaggcgcg agcgcagcgc gcagcgcgtc gtagtcgtct acctgagcgt gctccgtgat
    14881 ctcgatcacg actcgctctg cgacgtccag cagcagctcg cagaagcgag tatcgagcgc
    14941 ggtcgcgggc gacacgttga gcgcaatcgc gacgtcggcg ggaaactcgt ctagacgcag
    15001 cagcgccgcg cgcatcgctg cgagctcgag ctcgacaccc agcccgacct cggtcgcctc
    15061 ggcgaaccaa gctgaaggcg gacgcatcgg ctcggtccag aagcgcgcga gagcctcaag
    15121 cgaaacgatg cggcagtcga gcaggtcgaa gatgggctgg aactcgatgt ccatgtgatc
    15181 ggagtcgagg atcgcgcgaa tgcgggacca ctccagcttg cgcttctcgc cctcgagctg
    15241 ctctcgatcg acctgatccg cgacgatctc cgccagcacg cgcagaaaac gcacgtcgcg
    15301 atcgttgagg gacggctcgg cggaccggct gaggcagcag agcgtgccgt aaagggttcc
    15361 gtcccagagc cggatcggag cgccgatgta cgccccgatg cccgactcgc gtgtcagcgg
    15421 gaggtcggcg gtgcgctcgt cggcggcggc gtcgtgaatg agatgaggca agtcgccttg
    15481 ggtcatgcgc ttgcagtagg tctgctcgac gggaaggcgc gtgccgatct cgaggtcgaa
    15541 ccactcgttg tcgccgtcca tcgcctccac gacgaagtcg ccgtcgaacg cgcccaggag
    15601 agccacgtcc atgtcgagcg ctgcgcgcgc gagcgagagg atccgctcga ttggctcgct
    15661 ggatccgtcg agactgaggc gcgcgagctc ggagagccct tcgagggtcg gcacaggctt
    15721 cgtggaggcc acgccgctca tgaggcgatg ctcgcttggc gcgggacgag ccgggtgacg
    15781 gttgccgtgc gtggagcgtc gaggatcgcg tcgatcagct tgagcgccgg cgtgtccttc
    15841 ccgatgcagc gctcggcgcc cgctgccagc gcgtggaacg cgatctgagg cgagcgcgcc
    15901 gcagtgacca tcacgacgcg cgaggccggc gagacccgtc gaacccttcg cgtcgcctcg
    15961 atgccgtcca tgctcggcat gtgcacgtcc atcagaacga catcgggacg cagcgcttcg
    16021 gcgagcgaga cggcttcgac gccgctcgac gccgacccta tgacctcgat gcgctcgtcg
    16081 attccgagcg tcagcatcaa cgcctcggcg aacaacgcgt ggtcgtcgac gacgaggacg
    16141 cgtacgactt ccttacgatc gacttcgctg ctgtgtaggt gtcgcattgg cggagaagaa
    16201 cgcgttctct ccgcaaactt gcgtcgctac gcgcggaatc actcgttcga gggaacgcga
    16261 acgtagatag ccgccctggc gtccagcgcc gcgcgccgga aggaacggtc tcggtcgagc
    16321 cgttgctcgg gcttgctgcc gcgcgcgacg acgacggccg ttgcatcgcg cagcagcgca
    16381 tccccggccg ggttggcgct gagccagtcg aggtacaggc gctggtcgcg gcgcgagaac
    16441 gcatcgagcg gattcgcgat ccagacgcgc cgcccgtcga gcgccagctg ttccgcgtcc
    16501 atcccgtccg cgaggatcgg cttgccggcg gccagccgca cggcctcact gcgtacccgc
    16561 gccccggcgc cgtactgttg cggtgtctgc agcagcccga ggacgaggaa ggccgccgga
    16621 acccacgcgc acagcacgag cgtgcgcggc gacaccgaga aatcgcgcag gtaccgcttc
    16681 ccgagaccat gtgcggcggg agccgcgatg aagcagagaa gccagacgct gttccgcccc
    16741 gcgtgcagtg tcagccctgc gaaggccgcg atgcaggcga gctcccacac tctcgcgcct
    16801 gagcggaacg cgaagaccag caacgggatt gccagcacga tgaagaggac gtcgaacggc
    16861 ttgccgatgg acagtggcgc ccacagccct tcgccgcgct gagcagcctc gctgccgagt
    16921 acgccgaggt aatagtggcc ggagcccagg agcgctgggg tcacgaagag cgccgcgcag
    16981 caagcgacga ggacactcgc cgcgacgacg ggctcttggc gcgcgcgctc gagcacgaga
    17041 taggcagcgg cgatcgcaag cccgaggagc accccaccgt ggaggtttgc ccaaagcacg
    17101 atgagcggca cgaggagcca cacgcgccga cttgacctcc gggactcggc gcgcagcaga
    17161 agcagcgccg cgcagaacag aacgagcgag aagagctgcg cgcgcacgat gaggaaggac
    17221 gggaccgcgg cgaagaagac aagcatcagc accaccgcgc tcgcggcgtc ggatgcgccc
    17281 agagcacgca taccgagagc gagcagcgtc agtgccgcgg tcacagcgat gagctgcgcg
    17341 accagaaagc ctcgatcccc gcccatgacc tggagcgcgt ggaagacgag ctcgcccagg
    17401 accggcacgt tgacccagtc caccgacggc gccgcggcat aaggaacgcc cgaagggatc
    17461 gagccccgct cgagaattgt cccgccaagc gcagcgagcc atctggcgtc agccccgatg
    17521 ctgccgagga gcgcgcaggc gcccgcgcct agggcgacag caagccaggt aagcctggag
    17581 ctctgcggcc tgggctccat ggaagaggca tcggcctcga aacccggccg ctgtaatttc
    17641 ggctaggaaa cccgctcgaa cagggcagcc tggccctggc ccactccgac gcaaagagtc
    17701 gcaaggccgt agcggccacg gcgccgccgc agctcgtgca gaagggagac cacgagccgt
    17761 gctccgctca tcccgagcgg atggccgatg gcgatagcgc cgccgttgac gttcaccctc
    17821 tcctcgtcga gccccaactc cctgatgacg gcaatgctct gcgatgcaaa cgcctcgttt
    17881 agctccacga gatcgagctc gtccaccgac acacccgcgc gagcgaggag cttgcgcacg
    17941 gcgggcaccg gcccgattcc catcacacgc ggatcgacgc ccgcgaccgc actggcgacg
    18001 aagcggccga gcggctcgac cccgagctcc ttcgccttct cctcgctcgc gatcaccagt
    18061 gctgccgcgc cgtcgttgat accgctcgcg ttgccggccg tgaccgtccc gccttcgcgg
    18121 aaggcaggct tcagggctgc gagcttctcg gcgctcgtgt cgggacgcgg gtgctcgtca
    18181 cgcgcgactc cactgactcc gacgagctcg tcgtcgaagc gaccggcttg cgccgcagcc
    18241 gcccaacgct gctgagagcg cagggcgaaa gcgtcctgct cttctcgcga cacgttccaa
    18301 cgctcggcga cgttctcgcc cgtctcgccc atcgactcga gcgggaacat cttctcgagc
    18361 cgcggattgg gaaatcgcca gccgagctgg gtgtcgtacg tagtcccgtc gccgtactca
    18421 ggtttggcgg tcacgagcgg cgcgcgactc atcgactcga cgccgcccgc gacaaagagg
    18481 tcgccgtccc ccgcggcaac ggcgtgacag gccgagacga ccgcagacag acccgacgcg
    18541 cagagtcggt tgaccgtgat tccggcgacg gtctcgggca ggcctgcgag caggacgccc
    18601 atgcgcgcca cgttgcggtt gtcctcgccg gcctgattgg cagcaccgaa gtagacgtcc
    18661 tcgatctgtt ccggcggcac acccgcgcgc gcgaccgcct ccgcaattac ggtcgcggcc
    18721 aggtcgtccg gccgcaaagc ggcaagcgcg ccgtccttgc gtccgatcgg cgtgcggacg
    18781 cccgagagga tgacggcgcg gctcacgtgc cgacgaattc gattcgtacc ggctcttcgt
    18841 agacgcccgt cgcctcggcg cgccgtaaca gctcggcgac ggcctggcgc ccttcatcgc
    18901 cgtagtcgca cgtgagctcg ttcacataca tctcgatgaa gcggtccgcc gtttgcgcat
    18961 cgaggtcgcg cgcgaagccg agcgcgtact gcgtcgcctc ggcgcgattt gcgagcccgg
    19021 cctcgatcga ttcacggagc gcgacggaga gctcgtccag ccgcggcacg tcacggcgcg
    19081 ccacgttcac gccgagcggc agcggtaacc ccgtctcgag cagccaccac tcgccgaggt
    19141 cgagcacctt gacgagccct tcgtccgcgt aggtgagctg tccttcgtgg atcaacagtc
    19201 cagcgacggc gtgcccggcc ttgacctcgt cgagaatccg gtcgaaagcg agcgcccgat
    19261 gcgcgaaggg accgccgagc gccattccga gcacgaggaa cgcggtggtc aggcgtccgg
    19321 gaacggcgat ctcgacgtcg cgtaggtccc gcggcgacag ctcctcgcgc gcaacgacga
    19381 tcggcccgta gcccgacccg atcgacgcgc cgtgcggtag cagcgcgtat cggttctgga
    19441 cgagcggata cgtcgcgagc gagagcgccg tcacctcgag cctgccctcg agcgcccact
    19501 cgttcagaac ctgaatgtcc tcggggacga attcgaactc gaatcccctc gagtcgacgc
    19561 ggcccgaaac gaggccccac gccatgaacg cgtcgtctgg atctgccgaa tggccgatgc
    19621 ggatcagcac ggggtcccag cctagactgt gagcagatgg ctaagccggc actgacagac
    19681 gcgatccagg actacctgcg cgagatctac aagctcggcg ctgacggcgg ccgggtatcc
    19741 gtgactgcgc tggcaaagcg ccagggcgtc tccccggcgt cggccagcgc catggtgaag
    19801 aagctcgcgg cgctcgagct cgccgtccac cagccctacc gcgggatcac gctgacgcag
    19861 gacggcgaga aggtggcgct cgaggtcatc cggcaccacc ggctactcga gctctacctt
    19921 gcggagacgc tcggcgtcga cgtgaacgaa gtccacggcg aagcggaacg actcgagcac
    19981 gcgctgtcgg aagagctcga ggagcgtatc gacaaggcgc tcggattccc gacacacgat
    20041 ccgcacgggg accctattcc ggacgcgaat ctcgagtggc cctcgcagcc gacggcgtca
    20101 gccgtcggct caggccaacg agcgaagcga agttcttcct aatactcgcg gtttagcggc
    20161 gaccaaagaa ccaacgttcc aagggcttca acagctcacc gcgggtaaac ggcttcaggt
    20221 tctcggaacg tccgtcgctc tctgaaacgg tgaggtcgtc ctcgagcgcc ccgttcgcgg
    20281 cgtccaacgc gtcgtcgagc tcatagcctt cgaagaggta gtcgtcgcgc cgcggctcgt
    20341 cgctcgcgcg gttctcgggg ttgacggccc ggcccacgcc ccagagcgcg ccttgcttgc
    20401 gctcgatcca gatgacgatc gtctccgtgg cgcccgcgtc gtcgactccg tggacggtcc
    20461 tctcggcgaa gcgaccttcc gtcgttttcc gcctcacggc agcaaggata caattgcccg
    20521 atgcagcgat tcgcaagcct gctgcgctcg ccgttctctt ttctcttcgc ccgttcgagc
    20581 accgaggagc gtgtggctgc gtacgtcatc cgcgagcacg gccgtggccg cgggctcgcc
    20641 gagatcctgg aggaccggta cgtgcagaac cgtctcagct cgcaacagcg gctgcggctg
    20701 ctcgaccgtc ccgagttgat ccaagcgctt ggcgacgaga cggtcgagga cacacgtcgc
    20761 gacctcgaga gcgcgcgcag ctagagcttc ctgccgacgc cccgctgacg cgcgcgttcc
    20821 accacggcgg ctgcaacggc aacatcccag gaggcgatgc cgttcgactt gaagacgacg
    20881 atgtcgtccg gtgactgccg ccccggcagc tcgcccgcga ctacttcgtg cagctggtgg
    20941 acctccagcc agtccagcac tccttgctga atcggctcga tcaggtcggc ggactcgatt
    21001 cgcgcctgct ccttccaatc gcagcagacg aacgacgcgc gctccagcac gacgttgtcg
    21061 agctcgcgcg ctgccgggtt gttggccccg gccgcgcaca cgagcgcacc cggtctcagc
    21121 cattccccgc gaagcacggg gtcgcgagaa gtcgtgatcg tcacgacgac gtcctgctcc
    21181 gccgcttcgc ggttgctctc ggcgggctcg gcgtcgaccc gtttgcagaa tgcgagcagg
    21241 ttgcgctccg tacggcagta gacgacgacc ctctcgatcg ccggcacggc ggcacggatg
    21301 cacttgacct gcgtttccgc ctgcgtcccg cagccgatca ctccgagtga cgacgcgccc
    21361 tcgcgtgcga ggtgcttagc gccaacggcg ctcgccgccc cggtgcgtaa gcgtccgagg
    21421 tgatccgcct cgatcaaggc gacgagctcc ggccggtcga ccgcgaagag cgcaacgaca
    21481 aaagccgcac cctccgcaaa accggcatag accttcgcgc acgcatagcc cagctcgagg
    21541 tcggaggcgg ccatgtctgc gaggcgcccc tcctggagct tcagccgccg ccggggtgcg
    21601 atctcaacgg acccggcggc catgcgtagg aacgctcttt cgaccgcgtc aaccgcggct
    21661 tcgggtgtga gcagctcctc tacgtcgctc tccgaaagaa agagcgtcac tcgagctcgg
    21721 tgccgtcccg aacgacgatt cgatcgccct cgatccggac caccgcttcc cagggaaccg
    21781 ccttcggcgg gcgggccgag ggccggagga tcccaagatg ctcgagccac ccgcgtccgc
    21841 cgacgacgac tgcctctacc tccggccggc caccaccgag cgatgcacgg aggtctcggc
    21901 acgcacccag cttccggccc gactcggtct cgaccatgcg gccgagaaag gaggagaacg
    21961 tcctcaccgg tctgctccgg ggatcttctg gatgagaggc cgcagacgat cgtccccgcg
    22021 tccgagcccg agctcgtccg cgcgcttgcg caacttcacg tgcgccgaga cgacggcaac
    22081 ctcttcccag gggatccgca cgaccctgcc gccgccgatc caagctgcga agcgtccggt
    22141 gagccccgcg cgccgacgcc acgcgccggg cccgctgaag atcgcgacga cctccagctt
    22201 ttcgccgggc ctgccttcga gcgcgagatc atcgacgttg ccgcacctcc ggtcctccga
    22261 gtccagcagc tggcgatcga cgagctccag accgaggacg atcgtcttgc tcaattctgg
    22321 cccgcgttgg tgaggatgag tagtggcacc gcagtcaatg cgatgaccac gatcacgacc
    22381 agatagaaca gcccgagcac attcgcaagg cgtccattca cgtactcgcg catgtaacta
    22441 cggtcattcg cgaccagcag gatggggatg tacgtgagcg ggagcgcaac ggcggagaag
    22501 atcacggcgt attcggtcag ttggactgga tcgacgccgg tcatgacgag cccaagcccg
    22561 agcacaagca gcacgatcca ggtcagcgtg aagcgcggtg caccgctgtt gctccggtag
    22621 cgcccccact cccaaccgag gaactgcgcc aggttgtacg cacccgaaaa cagcgtgtcg
    22681 atcgccgcgc cgccgaccgc gaagaggatg ccgacgagag cgagcaggag cccgatctgc
    22741 ccgagtggaa cctcggcgcc caacgcaatc gagccgagga actcgggctc gatccccttg
    22801 ccgaggaaga gcgcgccgcc gacgatcatc agcgcgaagg agaggaaccc gccgagccca
    22861 tagccgatca tcgcattcgc cttgttcaga ccgagatcct tcggccccca ctgctcttcg
    22921 acggcgcccg aggaatagaa atacacctcg tatggcgtca tcgcggctcc cagaagtcca
    22981 accgcgaagt acgcgtagag gagagggctc tgcgtcggca cgtgggggac gaagccgtgg
    23041 ccgacggcgc cccacgcggg atgcagcttg accgcagcga ccgcaaacac gagcaggcag
    23101 agaccgccgt agccgaagat gcgctcgatc cagtcgaacg acgtggtcca cacgacaatc
    23161 gacagcacga cgccggcgat cacgatcagc cagcggtacg ggagcccgga cagcaactgc
    23221 aggcagatcg cgaccccgcc gatctccgcg gtcagagtca tgagattgac cacctgtgca
    23281 ctgacgagtg ctgccagccc ggttcggaag ccgagccgct ctcgcaccgc atcgaagacc
    23341 gccttgccgg acaccgtggc gacgcggccg cacatctccg agtagacgat gattccgacg
    23401 acgccgatga cgatgaccca gagcagttgg taaccgaagg tcgcgccggc agagacgttg
    23461 aagacgaggt cgccgatgtc gacgaagcca ccgatcgcgg cgaggattcc gagtgcaatc
    23521 tggaagatct tcatttcgcg agcttgtccg ccgcatcggc gtcgtccatg agcggctgcc
    23581 gcagcgaccg ggcgatatcg cggtccgtcg gatgaaggtg cagctgctcc agctctcggg
    23641 aaacctttcc cgccagcttc gacagcttgc ggacatcgga ccgtattccc ggcgacgtcg
    23701 ggcgcttggc gagcgagtcc tcgaaatccg cagcggcgcc cgacagctcc ttcgcgtgca
    23761 cgcgcacgaa gtactccgtg gatgcaccct tcgacacgtc gttggccatg agctcgcctt
    23821 cggttgcaag cgagcccacc ttctcggcat cacgcttcag ggcgtgcttg tcgacggtcc
    23881 ccccgttgca gcccgccagg gcaaacacca gcagggccga agcgactctc gcgatcagct
    23941 tcgccgagga gactcgagtt gaccgtccgt cttgcacgag ggtcacaggt tccccctcga
    24001 tcacttatga atccgtccgg atcgataggt gagcaacgcc cccgctacac tgatccgtcg
    24061 tctcagagcg cccgtagctc aggggataga gcgctgccct gcggaggcag aggtcgcaag
    24121 ttcgaatctt gccgggcgca ctccgctcgg aaatccgccg cgctgcgcgc ggcatgatga
    24181 tttccgagcg ttcgcattcg atccgtggcg tgcgatcgcg acgctagagt ccggcgattc
    24241 ctacgactct caggaggctg tttgcatgtc acccgcgggg aacaaggatc tgatccggcg
    24301 ctacatccag gcgatcgatg acaaccgcac gagcgactgg agcatcctcg acgagtacat
    24361 cgcagaggat ttcgtcgcgc acaatcctcc gattcccgga gtcagcctgg accgtgaggg
    24421 aatgaagcag gcggcggaga tctttcgagt tgctactccc gggcgtcacg agatcgggat
    24481 gcaggtcgag gaggatgatc tcgtcgtcag ccaaatcctc ggccgcggcg tgcacgcggg
    24541 agagctgctg ggaatccccg cgacgaacag agaggtcgag accgagggca tcgtgatcca
    24601 tcgcgtccgc gacggcaaga tcgtcgagta ctggtcggtg accgacgtcg tgcgggtgct
    24661 gcaacaggtc ggtgcgctgc cggggccgcc gcccggctag caccgtccct cggtgaggct
    24721 cgtcagcctc gctt
//
LOCUS       DASVEU010000216         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1508808,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000216 DASVEU010000000
VERSION     DASVEU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508808"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /gene="carB"
                     /locus_tag="VF225_09465"
     CDS             <1..2059
                     /gene="carB"
                     /locus_tag="VF225_09465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7145519.1"
                     /translation="TGDSWTVAPQQTLPDAEYQRLREAAFACARTVGVATGGANVQFA
                     YEPEARELVVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDELPNDITGKTTA
                     AFEPALDYVAVKAPRFDFAKFGVAKPRLGTEMRAVGESLGLGRTFAEAFLKALDGRER
                     APVELDLALRGEPVPERWDLLLESARRGLDLPGVHAYFADELRAVAHAERELATTGDV
                     AAAKRFGLSDPTVASILEVGEDEVRRRRARPGRLAVDSCAAEFEARTPYFYLSYEASD
                     PVPQGLRPAIVILGSGPNRIGQGIEFDYCCSRAAGAFRRLGYETVLLNSNPETVSTDY
                     DTSDRLYLEPLTLERTLDVCELEQPAGVVVQLGGQTPLALAPGVAGAGFPLLGDPLPA
                     IECAEDRGRFGALLGDLGLRAPAWGTATTGAEARAVAERIGYPVLVRPHHVLGGRGIR
                     VATGPDELELDEPALVDEFLAGALELDVDLLCDGESAWVGGILEHVEPAGVHSGDSAC
                     VIPAPSVIPLLESEVRELTAAIAIGLGARGLLNLQLAVYRGELYVLEANPRASRTVPF
                     LAKATGIPLVDHACRLLLGAGLEELDLPARAAPARAWAKEAVFPEERFANAADRGPEM
                     RSTGEVMASGDTVSQAYARVLRASGRARRGGSIGLALQTGGGSPPRSRGHELVSARLG
                     SGA"
     gene            complement(2031..3416)
                     /locus_tag="VF225_09470"
     CDS             complement(2031..3416)
                     /locus_tag="VF225_09470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX7145520.1"
                     /translation="MSATPKHSPEPEVWSPSSWRAYAALHQPEWPDAEKVEAAGARLT
                     SLPPLVFAGEARQLRDALAEAAAGRAFVLQAGDCAESFHDFSAPLIRDKLKILLQMAA
                     VLTYGGMLPVVKIGRIAGQFAKARSSPTETVADVELMSFRGHMVHGDEPTLAARVPDP
                     ERMLQVYHQSAATLNLLRAFTKGGFADLMQVHAWNQEFVARSPEGRRYEVIASEIERA
                     LRFMAACGIDLTSAPQLHQIDFWTSHEGLVLDYEQGLTRRDSLTGDWYDCSAHMLWIG
                     ERTRLPDGAHVEFFSGVNNPIGCKIGPTSTPEEVLLLCEQLNPERIPGRLTLISRMGA
                     DRVFELLPPLLRAVRESEHPVAWVCDPMHANVFTTDSGLKTRHLNSVMGEIEGFFAAH
                     RQEGTWPGGVHLEFTGEDVTECLGGSEEVHEDELNLRYMTLCDPRLNARQSLDLAFRV
                     AELMRRYPTAR"
     gene            3651..>4531
                     /locus_tag="VF225_09475"
     CDS             3651..>4531
                     /locus_tag="VF225_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145521.1"
                     /translation="MAPIVGLYLFGPEAFNAGPVFNAVGLAAVVAIVVGVRLHRPSDT
                     LPWYLFAAGQALFVCGDVIAYNFERFFGRPLPFPSIADVFYLAVFPVIVAGLLLLIRR
                     RKRGRDRASLIDSLIIATGVGLLSWVFLIAPYAHDGSLSTPARLTSMAYPLMDLLVLS
                     VAVRLAVSGGNHGRSYYLTITSIAALFASDATYGWLQLHSSYEPGGLLDGGWIAFYLL
                     WGAAALHPSMAVAKGTAKPTQLTRTRLVMLALAAFVAPITGLLGPVTRSDRIVITGCA
                     IILFSLVLLRMVELVQG"
BASE COUNT          680 a         1524 c         1547 g          780 t
ORIGIN      
        1 caccggcgac tcctggacgg tcgcgccgca gcagaccctt cccgacgccg agtaccagcg
       61 gttgcgggag gcggcgttcg cctgcgcgcg caccgtcggg gtcgcgacgg gcggagcgaa
      121 cgtccagttc gcgtacgagc cggaggctcg cgagctggtc gtgatcgaga tgaacccgcg
      181 cgtctcgcgc tcctcggccc tcgcctcgaa ggcgaccggc ttcccgatcg cgaagatcgc
      241 cgccctgctc gccgtcggct acacgctcga cgagctgccg aacgacatca ccggcaagac
      301 gaccgccgcc ttcgagccgg cgctcgacta cgtggccgtc aaggcgccgc gcttcgattt
      361 cgcgaagttc ggtgtcgcga agccacgcct cggcaccgag atgcgtgcgg tcggcgagtc
      421 gcttggtctg ggacggacgt tcgcggaggc attcctgaaa gcgctggacg ggcgtgaacg
      481 agccccggtc gagctcgacc tcgcgcttcg cggcgagccc gtacccgagc gctgggacct
      541 gttgctcgag tcggcgcgcc gtggtctcga tctgcccggc gtgcacgcgt acttcgcaga
      601 cgagctacgc gccgtcgcgc acgccgagcg agagctcgcc acgaccggag acgtcgccgc
      661 cgcgaagcgg ttcgggctgt ccgacccgac cgtggcaagc atcctcgagg tcggcgagga
      721 cgaggtgcgg cggcggcgtg cgcggccggg ccgtctcgcc gtcgactcgt gcgccgcgga
      781 gttcgaggcg agaacaccgt acttctacct ctcgtacgag gcgtccgacc ccgttccgca
      841 aggcttgcgc ccggcgatcg tgattctcgg ctccggcccg aaccggatcg ggcagggaat
      901 cgagttcgac tactgctgct cacgggcggc gggggcgttc cgccgtctcg gttacgagac
      961 ggtgctgctc aactccaatc cggagaccgt gtcgaccgac tacgacacga gtgaccggct
     1021 ctatctcgag ccgctcacgc tcgagcgcac gctcgacgtt tgcgagctgg agcagcccgc
     1081 aggtgttgtc gtgcaactcg gcggtcagac gccgctcgct ctggcgccgg gcgttgccgg
     1141 cgccggcttc ccgctgctcg gcgatccgtt gcctgcgatc gagtgcgcgg aggaccgcgg
     1201 tcgcttcggt gctctgctcg gagacctcgg cctgcgtgcg ccagcctggg gaaccgcaac
     1261 aaccggggcc gaggccaggg cggtcgccga gcggatcggc tatccggtgc tcgtacgtcc
     1321 acatcacgtg ctgggcgggc gcgggatacg agttgcgacc gggccggatg agctcgagct
     1381 cgacgagccc gcgctggtag acgagtttct cgcaggcgcg ctcgagctcg atgtcgacct
     1441 cctctgcgac ggggagtcgg cgtgggtcgg cgggatcctc gagcacgtcg agccggccgg
     1501 cgttcactcg ggcgactcgg cttgcgtcat ccccgcgccg tcggtgatcc ctttgctcga
     1561 gtccgaggtc cgtgagctca ccgctgcgat cgccatcggg ctgggtgcac gcggactgct
     1621 caacctgcag ctcgcggtgt accgcggcga gctctacgtg ctcgaggcga atcctcgagc
     1681 gtcgcgcacc gttcctttcc tggcgaaggc gacgggaatc ccgctcgtcg accacgcgtg
     1741 ccggctgctg ctcggcgccg gcctcgagga gctggatctc ccggctcgcg ccgcacccgc
     1801 gcgagcgtgg gcgaaagagg cagtcttccc ggaagaacgc ttcgcgaacg ccgccgatcg
     1861 ggggcccgag atgcgctcga ccggtgaggt gatggcgagc ggggacaccg tgtcgcaggc
     1921 ctacgcgcgg gtgctacgcg caagtggacg agcccggcgc ggagggtcga tcggcctagc
     1981 tctccagacg ggcggaggat caccgccgcg ctcgcgaggt cacgagctgg tcagcgcgcg
     2041 gttgggtagc ggcgcatgag ctcggcaacg cggaacgcga gatcgagcga ttggcgcgcg
     2101 ttcagtctcg ggtcgcacag ggtcatgtaa cgcaggttca gctcgtcttc gtgtacttct
     2161 tcggacccgc cgaggcattc ggtcacgtcc tcgccggtga attcgagatg gactccgcct
     2221 ggccacgtcc cctcttgccg gtgcgcggcg aagaagccct cgatctctcc cataacggag
     2281 ttcaggtgac gcgtcttgag ccccgagtcc gtcgtgaaca catttgcgtg catcggatcg
     2341 cagacccagg caacgggatg ctcggattca cgaacggctc gcagaagtgg cgggagcaac
     2401 tcgaagacgc gatcggcacc catacgggag atgagcgtga gccgaccggg gatccgttcc
     2461 ggatttagct gctcacagag gaggagcacc tcctcgggag tcgatgtggg gccgatcttg
     2521 cagccgatcg gattgttcac ccccgagaag aactcgacgt gagctccgtc cggcagccgc
     2581 gtgcgctcac cgatccagag catgtgcgcc gagcagtcgt accaatcgcc ggttagcgag
     2641 tcgcgccgcg tgagaccctg ctcgtagtcc agcaccaaac cttcgtggct cgtccagaag
     2701 tcgatctggt ggagctgggg cgcggacgtc aggtcgatcc cgcaggccgc catgaagcgc
     2761 agagctcgct cgatctccga cgcgatcacc tcgtaccgtc gtccctcggg ggatctcgcg
     2821 acgaactcct ggttccaggc gtgcacctgc atgagatcgg cgaagccgcc cttggtgaat
     2881 gcacgcagca ggttgagcgt tgccgccgac tggtggtaga cctgcagcat ccgctcggga
     2941 tcggggacgc gcgcggcgag tgtcggctcg tccccgtgca ccatgtgtcc acggaaggac
     3001 atgagctcca cgtcagccac ggtctccgtc ggcgaggagc gggccttggc gaactgaccg
     3061 gcgatgcgcc cgatcttgac gacgggaagc attcccccgt aggtcagcac cgccgccatc
     3121 tgcaagagga tcttgagctt gtcgcggatg aggggtgcgg aaaaatcgtg gaacgactcg
     3181 gcgcagtccc cggcctggag aacgaacgca cgtcccgctg ccgcctcggc gagggcgtcg
     3241 cggagctgcc gggcttcccc tgcgaagacg agcggcggga gcgaggtcag gcgggcgccg
     3301 gcggcttcca ccttctcggc gtccggccac tcgggttgat gcagcgctgc gtacgcacgc
     3361 caggacgagg gcgaccagac ttccggctcc ggcgagtgct tgggcgtcgc gctcatcgtt
     3421 gaagtctaaa gacgagggtg tgccctgaaa cgccaagtcc tgcgaacccg agggcccaca
     3481 ggacttgacg aaacgactct ctcacgtcgg gcggccgatt gcaagcggcc gtgggcgtgc
     3541 cccttctggg gggcatcggc tacgcggttc cgcggtacag ggcggctgcg ttctcgtcga
     3601 tggcgaagca agcgatgaaa actcgggcct ggtggctttt cctcgtagtg atggctccga
     3661 ttgtgggcct ctatctgttc ggcccggaag cgttcaacgc cgggccggtc ttcaacgctg
     3721 ttggtctcgc ggcagtcgtc gcgatcgtcg tcggcgtgcg ccttcatcgt ccgagcgaca
     3781 cgctgccctg gtacctgttc gcggccggcc aggccttgtt cgtctgcggc gacgtgatcg
     3841 cctacaactt cgaacgcttc ttcggccggc cgctgccgtt tccctcgatc gccgacgtct
     3901 tctacctcgc cgtcttcccg gtcatcgtgg ccggcctcct cctcctgatc aggcggcgaa
     3961 aacgaggccg agaccgggcg agcctcatcg actccctgat catcgcgaca ggggtagggc
     4021 tcctctcgtg ggtcttcctg atcgcgccct atgcgcacga cggcagtctc tcgacgccgg
     4081 cgcggctgac atcgatggca taccccttga tggatctcct cgtcctgtcg gtggccgtcc
     4141 ggcttgcggt cagcggcggc aaccatggca ggtcctacta cctgaccatc acgtcgatcg
     4201 cggccctctt cgcgtccgac gccacctacg gatggctgca attacattcc tcatacgagc
     4261 ccggcgggct tctggacgga gggtggatcg ccttctatct gctgtggggt gctgccgcgc
     4321 tccacccgtc gatggccgtg gcgaagggaa cggcaaagcc gacgcagctc acccgtacac
     4381 gcctggtcat gctcgctctc gccgcattcg tagccccgat caccgggctg ctggggccgg
     4441 tgacgagaag cgacaggatc gtgatcaccg gctgcgcgat catcctcttc agcctcgtcc
     4501 tcctccgaat ggtcgagctc gtccagggac a
//
LOCUS       DASVEU010000217         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1120530,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000217 DASVEU010000000
VERSION     DASVEU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120530"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VF225_09480"
     CDS             <1..798
                     /locus_tag="VF225_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX7145522.1"
                     /translation="RHILNGLHSVGLPWSWSIVALTLLVRMLLVPLTVKQIHSMQNMQ
                     AHAPQMKEIQRKYKGDRQKLNEELMKFYKENNINPAASCLPLVAQLPIFIGLFYTLRA
                     FAKHPPGGQAAIDRGDFSWLHFVPDITKHANSHWSGYVLLAIYVLSQVASTYFMSGTM
                     QKSQRYLMMILPFVFIPFIVKFPTGLVLYWMTTNLWTVGQGLITRRLMPKVGPGSAPP
                     PSGPKRSSRTPPKEPPADGGTQTAEAPPPKPSNGPPRRVKRKKKARR"
     gene            801..1499
                     /gene="jag"
                     /locus_tag="VF225_09485"
     CDS             801..1499
                     /gene="jag"
                     /locus_tag="VF225_09485"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX7145523.1"
                     /translation="METQEISVEATGETVGEAKWAALRELERQHPGLDKSAVHFQVVT
                     EGERGLLGVGFSPAKVVATISAGAAATEPADESELAAEARALVTRIVDGIGVTGRIEV
                     EEDDEVITVSCAGSDLGMLIGRHGQTIDAVQYLANAVMWRRHPEDRKEVVVDAAGYRD
                     RRRTALEALAVRSAERALSDQEPVELEPMTAVERKVVHLRLKEFNGVETTSEGTEPNR
                     FVVVIPAAEPPVRE"
     gene            1600..2130
                     /locus_tag="VF225_09490"
     CDS             1600..2130
                     /locus_tag="VF225_09490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX7145524.1"
                     /translation="MTGISELAEAHRMLLEDSLRGAGVVSEFEGSIVDVGSGGGVPGI
                     PLATSLPDREVTLLEAIGRKCDFLREWAPPNVRVVQGRAEEQETDVYGVAVAKALAKP
                     PVAAEWCLPLVRPGGAVVLWVGPSVDTKRVARVAAQLGGELAATGDGLLVLRKVRPTP
                     DGFPRRTGVASKRPLD"
     gene            2144..2896
                     /locus_tag="VF225_09495"
     CDS             2144..2896
                     /locus_tag="VF225_09495"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7145525.1"
                     /translation="MAKVYALANQKGGVGKTTTAINLAACLAEAGEPALVIDLDPQAN
                     ATSGLGERANGTSTYDLLDGVPLRDLAKPTKFPNLHLIPAKPELAGATVELTQHSDGE
                     TYLRQALASDIDQYSFVFLDCPPSFGPLTVNALAAADRVLIPVQAEYYALEGLSQLVQ
                     SINLVKARLNPRLAVGGVLLTMVDGRTRLSADVEAEVRKHFGELVFTQTVPRSVRLAE
                     APSHGLPAIAYDRRSRGADAYWKVAMELVDRA"
     gene            2889..3725
                     /locus_tag="VF225_09500"
     CDS             2889..3725
                     /locus_tag="VF225_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7145526.1"
                     /translation="MPERRGLGRGLEVLLGGATPSELAELPVDAIHPNPRQPRRRFDS
                     EAGAGLADSVRAQGVVQPVLVRPRTAGGYELIAGERRWRAARDAKLKTVPAVVREADD
                     RDTLLLGLVENVAREDLSPIEEARAFAVVLDEFGLSLGELAERVGKSKPAVSNRVRLL
                     ELPEDVLKMVERGQLTEGHARAVLAVPDHEGRRALARKIVQKGMSVRAAERAARWAGA
                     KTKQRRQTTKLDPALVARAKAAADRITGFSSRIVSNRLEIAFVDEPELEELVEALEKA
                     AK"
     gene            3766..4014
                     /locus_tag="VF225_09505"
     CDS             3766..4014
                     /locus_tag="VF225_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145527.1"
                     /translation="MLGLWLNDMESLEAISQDDEAKRIFLRMAALSRDGQMNNFLTEL
                     ARDEDLDDETKGTLKELAEDGTFLFAVEDYLHRTTVLH"
BASE COUNT          711 a         1330 c         1439 g          668 t
ORIGIN      
        1 cggcacatcc tcaacgggct gcactcggtc ggtctgccgt ggtcgtggtc gatcgtcgcc
       61 ctgacgctgt tggtgcggat gctgctcgtg ccgctgacgg tcaagcagat ccactcgatg
      121 cagaacatgc aggcgcacgc gccgcagatg aaggagattc agcgcaagta caagggcgac
      181 cggcagaagc tcaacgaaga gctgatgaag ttctacaagg agaacaacat caacccggcc
      241 gcctcgtgcc tgccgttggt ggcacagctg ccgatcttta tcgggctctt ctacacgctg
      301 cgcgccttcg ccaagcaccc tcccggcggc caggcggcaa tcgaccgcgg cgacttttcg
      361 tggttgcact tcgtgccgga catcaccaag cacgcgaaca gccactggtc ggggtacgtc
      421 ctcctggcga tctacgtgct cagccaggtg gcgtcgacgt acttcatgtc ggggacgatg
      481 cagaagtcgc agcgctatct gatgatgatt ctgccgttcg tcttcatccc gtttatcgtc
      541 aagttcccga ccgggctcgt tctctactgg atgacgacga acctctggac ggtcggccag
      601 ggcctgatca cccgccgcct catgccaaag gtcggcccgg gatccgcacc tccgccatcc
      661 gggccgaagc ggtcctcgcg caccccgccg aaggagccgc ctgccgacgg tgggacgcag
      721 actgccgaag cgccgccgcc gaagccgagc aacggtccgc ctcgtcgcgt caagcgaaag
      781 aaaaaggcgc gccgctagcg atggaaacgc aggagatctc ggtcgaggcg accggcgaga
      841 cggtcggcga ggcgaagtgg gcggcgcttc gagagctcga gaggcagcat ccggggctcg
      901 acaagtcggc tgtgcacttc caggtggtga ccgagggtga gcgaggcttg ctcggggtcg
      961 gtttctcgcc ggcgaaggtc gtagcgacaa tctcggcggg cgctgcggcg accgagccgg
     1021 ccgacgagtc ggagttggcc gcagaggcgc gagctcttgt gacacggatc gtggacggga
     1081 tcggtgtgac cggacggatc gaagtcgagg aggacgacga ggtcatcacg gtctcttgtg
     1141 ccgggtccga cctcggcatg ctgatcggcc gtcacggaca gacgatcgac gccgttcagt
     1201 acctcgcgaa cgcagtcatg tggcgacgcc accccgagga ccggaaggag gtcgtcgtgg
     1261 atgccgccgg ctatcgcgac cgccggcgca ccgcgctcga ggcgctggca gtgcgctctg
     1321 cggaacgggc gctttccgat caagagccgg tcgagctcga gccgatgacg gcggtcgagc
     1381 gcaaggtcgt ccacctgcgt ctcaaggagt tcaacggggt cgagacgacg agcgagggca
     1441 ctgagcccaa ccggttcgtc gtcgtgattc cggcggcgga gccgcctgtc cgcgagtagc
     1501 ggaccagggg agggggtccg gctgaaccgg gaggttccac cggctgattg cgcaaccctt
     1561 ctcgaaggct ggcttcgggc agttgttgcc accccaggtc tgaccgggat cagcgaactc
     1621 gcggaagcgc acaggatgtt gctcgaggac tcgttgcgcg gagccggcgt cgtgtcggaa
     1681 ttcgaggggt cgatcgtgga cgtaggctcg ggcgggggcg tgcccggaat tccgctggca
     1741 acgtcgctcc ccgatcgcga agtgacgctc ctcgaagcga tcggccgtaa atgcgacttc
     1801 ctacgcgagt gggcgccgcc caatgtccgc gtcgtccagg gtcgggcgga ggagcaggag
     1861 acggacgtct acggagtggc ggtagccaag gcactcgcca aaccacctgt cgcagcggag
     1921 tggtgccttc cgctcgttcg tcccggtggt gccgtcgtcc tctgggtcgg accaagcgtc
     1981 gatacgaagc gagtcgcccg ggtcgccgca cagctcggcg gcgagctcgc ggcaaccggc
     2041 gacggacttc tggttttgcg caaggtgaga ccgacgccgg acggctttcc gcgccggacc
     2101 ggcgtggcga gtaagcgtcc gttggactga gtacgctggc aacgtggcga aggtctacgc
     2161 gctggcgaat cagaagggcg gcgtcggcaa gacgacgact gcgatcaacc tggccgcgtg
     2221 cctcgcggaa gcgggcgagc cggcgctcgt catcgacctc gatccgcagg cgaatgcgac
     2281 gtcggggctc ggcgagcgtg cgaacgggac ctcgacgtac gacctgctcg acggtgtgcc
     2341 gctgcgagac ctcgccaagc cgacgaagtt tccgaacctg catctgatcc cggcgaagcc
     2401 ggagctcgca ggagccaccg tcgagctgac gcagcacagc gacggagaga cctacctgcg
     2461 tcaagcactg gcttcggaca tcgaccagta ctccttcgtg ttcctggact gtccgccctc
     2521 attcggaccg ctgaccgtga atgcgctcgc cgccgcggat cgcgtcctca ttccggtgca
     2581 ggccgagtac tacgcgctcg agggtctgtc gcagctcgtc cagtcgatca atctcgtgaa
     2641 ggcgcggctg aatccccggc tcgcagtcgg cggcgtcctg ctgaccatgg tcgacggacg
     2701 cacgcgtctg tcagcggacg tcgaagccga ggtgcgtaag cacttcggcg agctggtgtt
     2761 cacgcagacg gtgccgcgtt ccgttcggct cgccgaggct ccgagccacg gtctgcccgc
     2821 aatcgcgtac gaccgccgct cgcgcggcgc cgacgcctac tggaaggtgg cgatggagct
     2881 tgtcgaccgt gcctgagaga cggggcctcg gccgaggcct cgaagtcctg ctgggcggag
     2941 cgacgccgtc cgagctcgcc gagcttcccg tcgacgcaat tcatccgaac ccgcggcagc
     3001 cacggcggcg attcgactcg gaagcaggcg ccggacttgc cgactcggtg cgggcccagg
     3061 gcgtcgttca gccggtgctc gttcgcccgc gtacggccgg cggctacgag ctgatcgccg
     3121 gtgagcgccg ttggcgcgct gcgcgtgacg ccaagctgaa gacggtgccg gcagttgttc
     3181 gtgaagccga cgaccgggac acgctcctgc tcggcctcgt cgagaacgtc gcgcgcgagg
     3241 atctctcgcc gatcgaggag gcgcgcgcgt tcgcggttgt cctcgacgaa tttgggctct
     3301 cgctcggcga gctcgccgag cgcgtcggta agtcgaagcc ggccgtgtcg aaccgggtgc
     3361 ggctcctcga gctgcccgag gacgtgctga agatggtcga gcgcggccag ctgaccgaag
     3421 gccatgcgcg cgccgtgctg gcggtgccgg accacgaggg ccgccgcgcg ttggcacgca
     3481 agatcgtcca gaaaggcatg tctgtcaggg ctgcggagcg cgctgcgcgc tgggccggcg
     3541 ccaagacgaa gcagcggcgg cagacgacga agctcgatcc ggcgctcgtg gctcgtgcga
     3601 aggctgcggc cgaccgaatc accggtttct cgagccgaat cgtgtcgaat cgtctagaaa
     3661 tcgccttcgt cgacgagccc gagctcgaag agctcgtcga agcgctggag aaggccgcga
     3721 agtaagccgt atcccgttgg acgcggacct cgttcaacgg gtgacatgct cggcctgtgg
     3781 ctgaacgaca tggagtccct cgaggcaatc tctcaggacg acgaggcgaa gcggatcttc
     3841 ctccgcatgg cggcgctgtc ccgggacggc cagatgaaca acttcctgac cgagcttgcg
     3901 cgcgacgagg atctcgacga cgaaacgaag ggcacgctga aagagctcgc cgaggacggc
     3961 acgttcctct tcgcggtcga ggattacctc caccgcacga ccgtcctaca ctgaaaatcc
     4021 ggcgctgcgc gccatgatga ctttacttca gcaacgctaa cggcgagccg ccgtgtacga
     4081 gcaccggcag agccgccact cgcggcggga ctcgacgctc ctcgactgga ctctcttgta
     4141 ggaattcg
//
LOCUS       DASVEU010000218         6409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_926521,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000218 DASVEU010000000
VERSION     DASVEU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926521"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VF225_09510"
     CDS             <1..787
                     /locus_tag="VF225_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7145528.1"
                     /translation="KARLEVQLRRMQQWEERGYGQGRKKKKTKDVKKSIGGRIERLGA
                     VEKPYEPWELRLELTPQSRSGDVVVRLEGAVVERGSFRLGPLDLELSWADRLAIVGPN
                     GSGKTTLLDTILGRLPLAAGKRALGPGVVLGEVAQQRSELAEGQLLPAFTKAAGVNEE
                     SARTLLAKFGLGADHVLRDAGSLSPGERTRALIALLAARGVNCLVLDEPTNHLDVAAI
                     EELERALDVYPGTVLLVTHDRRFLAGFRATRRLELSPTPARSG"
     gene            complement(741..1514)
                     /locus_tag="VF225_09515"
     CDS             complement(741..1514)
                     /locus_tag="VF225_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145529.1"
                     /translation="MEELQKRRACLCRLTPDRALESQQEALEFLRDRGMLTRTPDSAL
                     PSLFEACHEEPYTAGSRGFGSWPATKYPWYFELAERPDVHELKVHNGKSILLTDESLA
                     LVDGVCRSELARMEDEAEWAPVLTHLAAAGPSTLTDLQIELGLAPRELKSLRAPLERC
                     GAVVSRSLRLQLPDGGHAHMSELARYDQAYPEPCGKGDVEELVIAAVRAAVVATETEI
                     VRKWFSWRRLFDDDLVERLVSEGRLTRIEPGWVTAPASA"
     gene            1586..1807
                     /locus_tag="VF225_09520"
     CDS             1586..1807
                     /locus_tag="VF225_09520"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX7145530.1"
                     /translation="MGMGVSLLLVAVGAILVWAVNATVSGIEIHTIGWILLAVGAIGA
                     LLSLIFWSSWGGFGGPADGRRRTVVREDY"
     gene            complement(1858..2484)
                     /gene="lepB"
                     /locus_tag="VF225_09525"
     CDS             complement(1858..2484)
                     /gene="lepB"
                     /locus_tag="VF225_09525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7145531.1"
                     /translation="MRGPIDRLFPNLPHGLRTVIDWVATIVGAVLIVLAVKAWVVNPY
                     RIPSPSMEPTLHCARPEPYCEAGSSDRVLANRFIYHFHSPRRGDIVVFHAPDAARREC
                     IGGIFVKRIIGLPGEAWSERNGVTYIDGRRLGEPYVGPTRRDSDTKTLRDIPPLGKLR
                     RIPAHMYLMEGDNRAHSCDSRVWGLVPRANIIGKVVLTYWPLNRLGTP"
     gene            complement(2492..3421)
                     /locus_tag="VF225_09530"
     CDS             complement(2492..3421)
                     /locus_tag="VF225_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7145532.1"
                     /translation="MRLALMIEGQEGVTWEDWVALASACEEHGVEALFRSDHYISWFD
                     ESRRVLDAWATLAGLAARTTKLELGTLVSPATFRHPSVLARSAATADEISGGRVTLGM
                     GAGWQEREHAAYGFEFGTVRERVARLGEQLEIAHDLLHEDRVDFDGTYYRLEDAPGLR
                     RPDLPILVGGSAKPGTARPAVRFANEYNALFGTLVEVRERKQLLDEACERGGRDPSTL
                     RYSLMAPCVIGRNEREVLASARRIGARFGRDPQEVLERNREHGPVGTIPEAVERLKQI
                     EEIGYERVMLQHLVHDDLETVALIGRELAPAVA"
     gene            3625..5769
                     /locus_tag="VF225_09535"
     CDS             3625..5769
                     /locus_tag="VF225_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7145533.1"
                     /translation="MAVASPEQYLADLNPAQREAVLTTEGPLLVIAGAGSGKTRVLTY
                     RVAHLINAVGAKPNEILAITFTNKAAGEMRERLQRLLGRSGQGLWILTFHAACGRILR
                     REAQRLGYRSNFTIYDQADQIRLVKQCLEELERDPKRFTPRGIHNQISHAKNRLISPD
                     EYANQVQSFYDQTVADVYQLYQRRLFASNAVDFDDLLMLTVDVLERFPDVREKWQQAF
                     RYILVDEYQDTNHAQYRLLQLLAEKHQNLMAVGDPDQSIYSFRSADINNILDFERDFP
                     GTRTVALEQNYRSTNLILEAANTVITNNRERKPKELWSELGDGEPVRVVEVEDEHAEA
                     RFVAAGIAGVVEQGFSGDEVAIFYRTNAQSRVLEDVLVRQGVAYQVIGGPRFYERAEI
                     KDAIAYLQAIDNPYDAVSLQRIANRPRRGIGDSSLARLQTWADAQGRSLWEAMEFAEE
                     AGVGAAPLKAVQGFRTLMQTLQSGALEFPVSELLERTLERSGYLEALEAERTIEALGR
                     LENLQELVGVAREYQETAEEPSLSHFLQEISLFSDQDSIRGEESLVTLMTLHNAKGLE
                     FRAVYLIGMEEGIFPHSRSIEEQGIEEERRLAYVGMTRAREQLTLTHASSRSLWGSRG
                     YNLPSRFLDELPSSVERERLRPASWSSYGAQGVAPREDIPSLSTGDSVRHGTLGEGVV
                     TQIEAGGVVTVRFADGSERRLMLDYAPLERIP"
     gene            5779..>6409
                     /locus_tag="VF225_09540"
     CDS             5779..>6409
                     /locus_tag="VF225_09540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145534.1"
                     /translation="MSIEIRNPVEDELRNAMKAVEAAFGEEQKEEDLDRHQKAMACDR
                     FLVAYDGAVPVGTTASLPFDLTVPGGELGAGGVTWVGVLPSHRRRGILTQLMKIQLQD
                     LHERGEPLAILWASEAAIYGRFGYGIAAPMVQLDADRSRFSLRDDPGPRGAVRLIDSH
                     EAGKVLPPIYDAVRRQVPGFTSRSAVLWDLYRLADPEHWRRGGGPKFYAV"
BASE COUNT         1049 a         2096 c         2200 g         1064 t
ORIGIN      
        1 gaaggcgcgc ctcgaggtgc agctgcgccg gatgcagcag tgggaagagc gggggtacgg
       61 gcagggtcgc aagaagaaga agaccaagga tgtgaagaag tcgatcggcg gccgcatcga
      121 gcggcttggc gcagtcgaga aaccgtacga gccgtgggag ctccggctgg agctcacgcc
      181 gcagagtcgc agcggtgacg ttgtcgtccg cctcgagggc gcggtggtgg agcgcggctc
      241 gttccggctc ggcccgctcg acctcgagct gtcctgggcc gaccgtcttg cgatcgtcgg
      301 cccgaacggg agcggcaaga cgacgctgct cgacacgatc ctgggccggc tgccgcttgc
      361 ggcggggaag cgcgcgctcg gcccgggtgt cgtgctcggt gaggtggccc agcagcgaag
      421 cgagctcgca gagggccagt tgctgccggc attcacgaag gccgcgggag tgaacgagga
      481 atcggcccgc actttgctcg cgaagttcgg cctcggcgcc gatcacgtgc tgcgcgacgc
      541 aggctcgctc tcgcccggcg aacgcacgcg tgcgttgatc gctctgctcg cggctcgcgg
      601 cgtgaactgt ctcgtcctcg acgagcccac gaatcatctc gacgttgcgg cgatcgaaga
      661 gctcgagcgt gcgctcgacg tttaccccgg gacggttctg ctcgtgacac acgaccggcg
      721 cttcctcgcc ggctttcgcg ctacgcggag gctggagctg tcacccaccc cggctcgatc
      781 cgggtgagcc gcccctcgct gacgaggcgt tcgaccaggt cgtcgtcgaa cagccgccgc
      841 cagctgaacc atttgcgaac gatctcggtc tcagtcgcga cgactgcggc gcggaccgct
      901 gcgatgacga gctcctcgac gtcgcctttc ccgcagggct cggggtacgc ctggtcgtag
      961 cgcgcgagct cgctcatgtg cgcgtgcccg ccgtcgggta gctgcagccg gaggctgcgg
     1021 ctgacgaccg cgccgcaccg ctcgagcggt gcgcgcaacg atttcagttc cctgggcgcg
     1081 aggcccagct cgatctgcag gtcggtgagt gtcgacgggc ctgctgccgc caggtgagta
     1141 agcactggtg cccactccgc ttcgtcttcc atgcgcgcaa gctcggaacg gcagaccccg
     1201 tcgaccaggg cgaggctctc gtccgtgagc aggatcgact tgccgttgtg gaccttgagc
     1261 tcatggacgt cgggccgttc cgccagctcg aagtaccacg ggtacttcgt ggccggccat
     1321 gaaccgaatc ctcggctgcc ggcggtgtac ggctcttcgt ggcatgcctc gaacaggctc
     1381 ggcagcgccg agtccggcgt tcgcgtgagc atcccgcggt ccctgaggaa ctcgagtgct
     1441 tcctgctggg actcgagtgc acgatccggg gtaagcctgc acaggcaggc gcgccgcttc
     1501 tggagctcct ccacggccgt atcttcgcag ggggtttccg cgttttccct gcagacacgc
     1561 ggattccatc gaaaggagac acaacatggg tatgggagtc agccttcttc tcgtcgccgt
     1621 cggggcaatc ctcgtctggg ctgtcaacgc gacggtgagc gggatcgaga tccacacgat
     1681 cggttggatc cttctcgcag tgggcgcaat cggcgctctg ctctcgctca tcttctggtc
     1741 gagctggggc ggtttcggcg gtcccgccga cggacggcgc cggacggtcg ttcgcgagga
     1801 ctactagacc cgagcaccat cttcgttcga gagccccgct tcggcggggc tctttcttca
     1861 tggggtcccc agtctgttga gcggccagta agtcaggacg accttgccga tgatgttcgc
     1921 gcgcggcacg agcccccaca cccgtgaatc gcaagagtgc gcgcggttgt ctccctccat
     1981 gaggtacatg tgcgccggaa tgcgtcgcag cttgccgagc ggcgggatgt cgcgaagcgt
     2041 tttcgtatcg ctgtcgcggc gtgtcggtcc gacgtaaggc tcgccgaggc ggcgcccgtc
     2101 gatgtaggtc acgccgtttc gctccgacca ggcctcgccg ggcaggccga tgatccgctt
     2161 cacgaagatc ccgccgatgc attcgcgccg cgccgcatcc ggcgcgtgga agacgacgat
     2221 gtcgcctctt ctcggggagt ggaagtggta gatgaaccga ttggccagca ctcgatccga
     2281 gctgccggcc tcgcagtagg gctcgggtcg tgcgcagtgg agcgtcggct ccatggaggg
     2341 ggaggggatt cgatacggat tgacgaccca ggccttgacg gcgagaacga tcagtactgc
     2401 tccgacgatc gtcgccaccc agtcgatcac agtccgcaac ccgtgcggca ggttcgggaa
     2461 gagccggtcg atcgggccgc gcaacgcggc gctacgcgac ggccggcgcg agctcgcgcc
     2521 cgatcagggc aaccgtctcc agatcgtcgt gcacgaggtg ctgcagcatg acgcgttcgt
     2581 agccgatctc ctcgatctgc ttgaggcgct cgaccgcctc cgggatcgtc ccgaccgggc
     2641 cgtgctcgcg gttgcgctcg agcacctctt gtggatcacg cccgaagcgc gcgccgatgc
     2701 gtcgcgccga tgccagcact tcccgctcgt tgcggccgat cacgcagggc gccatcagcg
     2761 agtagcggag ggtggaggga tcgcggccgc cgcgctcgca ggcttcgtcg agcagctgct
     2821 tacgttcgcg gacctcgacg agcgtgccga agagggcgtt gtactcgttg gcgaatcgga
     2881 ccgcaggccg ggcggtgccg ggtttggcac tgccgccgac gaggatcgga aggtcgggac
     2941 ggcggaggcc cggggcgtcc tcgagccggt agtacgtgcc gtcgaagtcg acgcgatcct
     3001 cgtggaggag atcgtgcgcg atctcgagct gttctccgag ccgtgcgaca cgctcgcgga
     3061 cggtgccgaa ctcgaagccg tacgcggcgt gctcgcgctc ctgccagccg gcacccattc
     3121 cgagggtcac gcgcccgccc gagatctcgt ccgccgtcgc cgcggagcgc gcgagcacgg
     3181 acggatgccg gaaggtcgcg ggagagacga gcgtgccgag ctccagcttc gtcgtgcgtg
     3241 cggccagtcc ggccagcgtc gcccacgcgt cgagcacgcg acggctctcg tcgaaccacg
     3301 agatgtaatg gtccgagcgg aagagcgcct cgacgccgtg ttcctcgcag gccgacgcca
     3361 gcgcgaccca atcctcccag gtcacaccct cctgtccctc gatcatgagc gcgagccgca
     3421 cgaagcggaa gcctatgcat gtccgcatcg gccgtgtgtg gacgaaaagt cacacttcct
     3481 gcacgatctc cttgtccctt cgagccggtt tgaccatcgt ctccgcggga cctgtcgggg
     3541 acaggccccg ccgtggctcg agtcgacatg gacgggcgag ccaggtctcg gctgccccaa
     3601 cagggccccg ctagcatcga ccccatggcc gtcgcctcgc cggagcagta cctcgcggac
     3661 ctcaaccccg ctcagcggga ggccgtgctc acgaccgagg ggccgctgct cgtcatcgcc
     3721 ggggcgggct cggggaagac ccgcgtcctc acgtatcgcg tcgcccacct gatcaacgcc
     3781 gtcggtgcga agccgaacga gatcctcgcc atcaccttca cgaacaaggc ggccggcgag
     3841 atgagggagc gcctgcaacg gctgctcggg cgtagtggcc aggggctctg gatcctcacg
     3901 ttccacgccg cctgcgggcg aatcctgcga cgcgaggcgc agcggctcgg ctatcgctcg
     3961 aacttcacga tctacgacca ggccgatcag atccggctgg tgaagcaatg cctggaggag
     4021 ctggagcgcg acccgaagcg gttcaccccg cgcggcatcc acaaccagat ctcgcacgcg
     4081 aagaaccggc tgatctcacc ggacgagtac gcgaaccagg tgcagtcgtt ctacgaccag
     4141 acggtcgcgg acgtctacca gctgtaccag cgacgcctct ttgcttcgaa cgccgtcgac
     4201 ttcgacgacc tgctgatgct caccgtcgac gtcctggaac gcttcccgga cgtgcgcgag
     4261 aagtggcagc aggcgttccg ctacatcctc gtcgacgaat accaggacac gaaccacgcg
     4321 cagtaccgac ttctccagct gctggccgag aagcatcaga acctgatggc ggtgggcgat
     4381 ccggaccagt cgatctattc gttcaggtca gcggacatca acaacattct cgacttcgaa
     4441 cgcgacttcc ccgggacacg gacagtcgcg ctagaacaga actaccgctc gacgaatctc
     4501 atcctcgagg cggcgaacac cgtcatcacg aacaacaggg agcgcaagcc gaaggagctg
     4561 tggtcggagc tcggggacgg ggaaccggtg cgtgtagtcg aagtcgagga cgagcacgcg
     4621 gaggcacggt tcgtcgcggc cggcatcgcg ggggtcgtcg agcagggctt ctccggggac
     4681 gaggtcgcga tcttctaccg gacgaacgcg cagtcgcggg tgctcgagga cgtcctcgtg
     4741 cggcagggtg tcgcgtacca ggtgatcggc ggcccgcgct tctacgagcg agccgagatc
     4801 aaggacgcga tcgcgtatct gcaggcgatc gacaacccct acgacgccgt ctcactgcag
     4861 cggattgcga acaggccgcg acgcggcatc ggcgactcca gcctcgcacg cctgcagact
     4921 tgggccgacg cacagggacg ctcgctgtgg gaggccatgg agttcgcgga agaggcggga
     4981 gtcggcgccg ctccgctcaa agcggtgcag ggattccgga ccctgatgca gacactgcag
     5041 tccggtgccc tggagttccc cgtttcggag ctgctcgagc gcacgctcga gcgcagcggt
     5101 tatctcgagg cgttggaggc cgagcgcacg atcgaagcac tgggaaggct cgagaacctg
     5161 caagagctcg tcggcgtcgc gcgcgagtac caggagactg cggaggagcc gtcgctgtcg
     5221 cacttcctgc aggagatctc gctcttctcc gatcaggatt cgatccgtgg cgaggagagc
     5281 ctcgtcacgc tgatgacgct ccacaacgcc aagggcctcg agtttcgcgc cgtctacctg
     5341 atcgggatgg aggagggaat cttcccgcac agcaggtcga tcgaggagca gggaatcgaa
     5401 gaggagcgac gccttgcgta tgtcgggatg acgcgggcgc gcgagcagct gacgctcacg
     5461 cacgcgtcgt cgcgctcact ctggggatcg cggggctaca acctgccttc gcgcttcctc
     5521 gacgagttgc caagctcggt cgagcgggaa cggctgcggc ccgcctcctg gtcgtcgtac
     5581 ggcgcacagg gcgtggcgcc gcgggaagac attccttcac tctccacggg agattcggtt
     5641 cgccacggca cgctcggcga gggcgtcgtg acgcagatcg aggctggcgg tgtcgtcacc
     5701 gttcgcttcg ccgacggaag cgagcggcga ctgatgctcg actatgcgcc gctcgaacgc
     5761 attccttagg gtctagacgt gtccatagag atccggaatc cggtcgagga cgagctccgc
     5821 aacgcaatga aggcggtcga agccgcgttc ggcgaagagc agaaggaaga ggacctggac
     5881 cgccaccaga aggcgatggc ttgcgatcgc ttcttggtcg cctacgacgg cgctgtcccc
     5941 gtcgggacga ccgcttcttt gccgttcgac ctcacggtgc cgggtggcga gctcggagcg
     6001 ggcggtgtca cctgggtggg agtcctgccg agccaccggc gccgcggaat cctcacgcag
     6061 ctgatgaaga ttcagctcca ggatctgcac gagcgcggcg agccgttggc gatcctctgg
     6121 gcttcggagg cggcaatcta cggccggttc ggttatggaa tcgctgcgcc catggtgcag
     6181 ctcgacgccg atcggtcgcg gttctctctt cgcgacgatc ccgggccgcg cggcgcggtg
     6241 cgcctgatcg acagtcacga agccgggaaa gtgctgccgc cgatctatga cgccgtgcgt
     6301 cgccaggtgc ctggcttcac gagtcgcagc gcggttctct gggacctgta ccgtctcgcc
     6361 gatccggaac attggcgccg cgggggcgga ccgaagttct atgcggtgc
//
LOCUS       DASVEU010000219         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_150270,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000219 DASVEU010000000
VERSION     DASVEU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150270"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VF225_09545"
     CDS             <1..1002
                     /locus_tag="VF225_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145535.1"
                     /translation="QLTMRTFHTGGVAGADITHGLPRVVEIFEARNPTGAATLAEVGG
                     KVEIEDTARGPKATIVPFDLKKDEEPKAIQLPRRTRLLVTKGQEVQPGDPLHEGSINP
                     ADLLRLKGYTETELYLIGEVQKVYKSQGVEIHDKHIELIVRQMLKKVRVENAGQTDLL
                     PGQLVDKTVLDRENTRIKKEKKDQATFEPLILGITKASLATESFLSAASFQETTKVLT
                     DASIEGKVDRLLGLKENVIIGKLIPAATGLKQYRGIEIGPSDKVPASAYMRPATEEQL
                     LAALEEIDSDGGNGIDLDSLGLDFGGTPGVEDGGSSGKSDIEAEEIPEVDSPLDKDQ"
     gene            1073..2830
                     /locus_tag="VF225_09550"
     CDS             1073..2830
                     /locus_tag="VF225_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145536.1"
                     /translation="MKRFGVLVAAVLALALAGGKAAPAQQLQANCSLFGGKTPLWLDF
                     ADGSVPFWNLFARPGVTALASNLIYPPKLREAGAQTAYFDLYLNRRVGTTSVPADPAT
                     IVDKANKFYEYAAQAMDCSNPVIAENELFGSWLAVPWSKTNAQYRANVLLFLQTLRQR
                     GAQPWLLVNAKPYTDSTAGDWWRQVADVAGIVREVYFPAPLIYKQGPIRGSRTLRQAF
                     RNGVLDFTKIGIPVSKLGIFLGFQTSKGSGGREGLEAKAWFRTVKWQALAARYVATEM
                     KFSSIWSWGWADWTTTPGEKDPAKPLAACVYLWTRSPSLCNGPRVAGKGFVRSRTEGQ
                     LILQPGLRCQLGHAAVRWSQINPILKLTGDPELAFSNAFARVVAQRSAQVGYGDILAA
                     ERSIISARFAGSRGAYLAAIADAKTNLSVARGIIGDELRRARIQSKFTVRSPSAREIA
                     DYHDTYGDLQARLVQAKSGAPWLGGRLSGYAVESAAPPRLMTLLSGRWSAVWSPLGTV
                     QVRPLGPPLPLGTLPLGNAREAIRAALMTQEREDRYPTWLTSAQQAAFPEAICWRDQM
                     PEMGEVDLTNYLPFLALTS"
     gene            complement(2874..4040)
                     /locus_tag="VF225_09555"
     CDS             complement(2874..4040)
                     /locus_tag="VF225_09555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7145537.1"
                     /translation="MTTILIGISVGLGALVVLLLALLVVRWRSDSSTDERVAEVVASL
                     QTRMDELGRELAGALERAEEEGRRSRIFGELAGSIDLDEVLSRTLEAAGALEGADAAL
                     IMLPDPAGGKPLVATLGLSVEEAERHAITGPPDGRLARSITMSYTYPELEREANPGEA
                     VIHAGLAVPLPGDNLTLGYLTVFTRKKGHDHGDDDVRQLETLAMRAGPAIENARRFRE
                     ARQLADLDALTGLHNRRYFHETLARECSRAHRYERKLSLIVFDLDDFKEINDRIGHLA
                     GDTVLAEAAERVRDVVRTADIACRVGGDEFAVILPESSLEDADQLYRRIQNAISTRPL
                     GQGGKLFLSAGVAELRAEDDPVAFFQRADDALYRAKEAGKGRVVSANSPRLGPA"
     gene            4298..4666
                     /gene="rpsL"
                     /locus_tag="VF225_09560"
     CDS             4298..4666
                     /gene="rpsL"
                     /locus_tag="VF225_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7145538.1"
                     /translation="MPTVNQLVRKGRALQKGKTKTPALGGAPQKRGVCTRVFTHTPKK
                     PNSALRKVARVRLTNQMEVTTYIPGEGHNLQEHPVVLVRGGRVKDLPGVRYKVIRAAL
                     DTAGVSDRKQGRSKYGAKKS"
BASE COUNT          808 a         1603 c         1580 g          738 t
ORIGIN      
        1 cagctcacga tgcggacgtt ccatacgggt ggtgtggccg gcgcggacat cacgcacggc
       61 ctcccgcgcg tcgtcgagat cttcgaggcg cgcaacccga cgggtgcagc cacgctcgcc
      121 gaggtcggcg gcaaggtgga gatcgaggac acggcccgcg gtccgaaggc gacgatcgtc
      181 ccgttcgacc tgaagaagga cgaggagccg aaggcgatcc agctgccgcg caggacgcgt
      241 ttgctcgtga cgaagggtca ggaggtgcag ccgggagatc cgctgcacga ggggtcgatc
      301 aaccccgcag atctcctgcg cctgaagggc tacacggaga ccgagctgta cctcatcggc
      361 gaggtgcaga aggtctacaa gtcgcagggc gtcgagatcc acgacaagca catcgagctg
      421 atcgtccgcc agatgctgaa gaaggtgcgc gtcgagaacg cgggccagac cgatctgctg
      481 cccggccagc tcgtcgacaa gacggtgctc gaccgcgaga acacgcgcat caagaaggag
      541 aagaaggacc aggccacgtt cgagccgctg atcctcggga tcacgaaggc atcgctcgcc
      601 accgaatcct tcctctcggc tgcgtcgttc caggagacga ccaaggtcct gacggatgcg
      661 tcgatcgagg gcaaggtcga ccggctgctc gggctgaagg agaacgtgat catcgggaag
      721 ctgatcccgg cggccaccgg cctgaagcag taccgcggaa tcgagatcgg cccctccgac
      781 aaggtcccgg cgtccgcgta catgcggccg gcgaccgagg agcagctcct cgctgccctg
      841 gaggagatcg actccgacgg cggcaacggc atcgacctcg actcgctcgg gctcgatttc
      901 ggcggcacgc cgggggtcga ggacggcggc agctccggca agagcgacat cgaggcggag
      961 gagatccccg aagtggactc cccgctcgac aaagaccagt agtaagccgt agccgttaac
     1021 tgttcgtaag cgaggccggg tgtcgcccgg cctcgcggct actgtaaggc gcgtgaagag
     1081 gttcggggtc ctcgtcgccg ctgtcctcgc gctcgcgctc gccggcggga aggccgcgcc
     1141 ggcgcaacag ctgcaggcga actgctctct cttcggcggc aagacgccgc tctggctcga
     1201 cttcgccgac ggctcggtcc ccttctggaa cctcttcgcc aggccgggcg tgactgcgct
     1261 cgcatccaat ctcatctatc cgcccaagct gcgcgaggcg ggtgcgcaga ccgcgtactt
     1321 cgacctgtac ctgaaccggc gcgtcgggac gacgagcgtg cctgccgacc ccgcgacaat
     1381 cgtcgacaag gcgaacaagt tctacgaata cgccgcccag gcgatggact gctccaatcc
     1441 ggtgatcgcc gagaacgagc tcttcgggtc ctggctcgcg gtgccctggt cgaagacgaa
     1501 cgcgcaatac cgcgcgaacg tgctcctctt cctgcagacg ctgcgccaac gcggcgcgca
     1561 accgtggctg ctcgtcaacg cgaagccata caccgatagc accgccggcg actggtggcg
     1621 gcaggtcgcg gacgtcgcgg ggatcgtccg cgaggtctac ttcccggccc cgctgatcta
     1681 caagcagggc ccgatccgcg gcagtcgcac gttgcgtcag gcgttccgaa acggcgtcct
     1741 cgacttcacc aagatcggga tcccggtctc gaagctcggc atcttcctcg gcttccagac
     1801 gtcgaagggc agcggcggcc gggaggggct ggaggcaaag gcctggttcc ggaccgtgaa
     1861 gtggcaggcg ctggcagccc gatacgtcgc cacggagatg aagttcagct cgatctggtc
     1921 ctggggctgg gcggattgga cgacgacccc gggcgagaag gatcccgcta agccgctggc
     1981 ggcgtgcgtc tatctctgga cccggagccc gagcctctgt aacggccccc gcgttgcggg
     2041 caagggattc gtgcgctcac gcaccgaagg gcagctgatc cttcagccgg gcctgcgctg
     2101 ccagctcggc cacgcggccg tgcggtggtc gcagatcaat ccgattctca agctgaccgg
     2161 cgatcccgag ctcgcgttct ccaatgcgtt cgcgcgcgtt gttgcacagc gcagcgcgca
     2221 ggtgggctat ggcgacatcc tcgcagcgga gcgctccatc atcagcgcgc gtttcgccgg
     2281 ttcccgtggc gcgtacctgg cggcgatcgc cgatgccaag accaacctct cggttgcgcg
     2341 cggcatcatc ggcgacgagc tcaggcgtgc gcggatccag agcaagttca ccgttcgcag
     2401 tccgagcgca cgcgagattg ccgactacca cgacacctac ggcgacttac aggcgcgtct
     2461 cgtgcaagcc aagtccgggg ccccctggct gggcggccgc ctttcgggct acgcggtcga
     2521 gtccgctgcg ccgccgcgct tgatgaccct gctgtccggc cgctggagtg ccgtctggtc
     2581 gccgctcggg accgtgcagg ttcggccgct cggcccgccg ctcccactcg gcacgctccc
     2641 gctcggcaac gcgcgggagg cgatccgcgc tgcgctgatg acgcaggaac gtgaagatcg
     2701 ctatccgacc tggctcacgt ccgcccagca ggcagcgttc cccgaggcga tctgctggcg
     2761 cgaccagatg cccgagatgg gcgaggtcga cctaacgaac tacctcccgt tcctcgcgct
     2821 gacgtcttag tagacggacg ggtccgaaac ggcaagggtc tgaccccggc tgtttaggcc
     2881 ggaccgaggc gcggcgagtt cgccgagacg acgcgaccct tgcccgcttc cttcgcgcgg
     2941 tagagggcgt cgtccgcacg ctggaagaac gcgacgggat cgtcctccgc acggagctct
     3001 gcgacgccgg cggacaggaa cagcttgccg ccctggccga gcggacgcgt cgagatggcg
     3061 ttctggatcc ggcgatagag ctggtcggcg tcctcgagcg acgactccgg caggatgact
     3121 gcgaactcgt cgcccccgac gcggcacgca atgtcggccg tacgcactac gtcgcggacc
     3181 cgctccgcag cctctgccag cacagtgtct cccgccaggt gaccgattcg atcgttgatc
     3241 tccttgaaat cgtccaggtc gaagacgatc agtgacagct tgcgctcgta gcgatgcgct
     3301 ctggagcatt cgcgggccag cgtctcgtgg aagtagcgcc ggttgtgcag gccggtgagc
     3361 gcgtcgaggt cggcgagttg ccgcgcctcg cgaaagcggc gcgcattctc gatcgccggg
     3421 ccggcgcgca tagcgagcgt ctcgagctgg cgcacgtcgt cgtcgccgtg gtcgtgcccc
     3481 ttcttgcggg tgaacaccgt cagatagccg agggtcaggt tgtcaccggg aagcgggacc
     3541 gcgagcccgg cgtggatgac tgcctcgcct gggttcgctt cacgttcgag ctcgggatag
     3601 gtgtaggaca tcgtgatcga ccgcgcgagc cgtccgtccg gcgggccggt gatcgcgtgc
     3661 cgctcagcct cctccacgga caggccgagc gttgcgacga gcggcttgcc gccggcaggg
     3721 tccggcagca tgatcagcgc cgcgtcggcg ccttcgaggg ccccggcggc ctcgagcgtc
     3781 cgagagagca cttcgtcgag gtcgatcgac ccggcgagct ccccgaagat ccggctgcgg
     3841 cggccttcct cctcggcccg ctcgagcgcc ccggccagct cccggccaag ctcgtccatc
     3901 cgggtttgca ggctcgccac cacctcggcg acccgttcat cggtggagct gtccgagcgc
     3961 cagcggacga cgagcagggc cagcaatagc actacgagag cgccaaggcc cacactgatc
     4021 ccgatcagaa tggtggtcat tcgtttgccg caggaaaggt aaaacccgcc gactcactgt
     4081 agtggcgctt gggcgctctc actagctttt gcaagtaaga aaatccagcc gcggcggccc
     4141 cgtcgaaaac gggcgaaaag cgcctctggt tccgctccag aagcgggttt cgcggtttgc
     4201 gggccctctc aggtttcgct accatgcctc gtccggttgg cagggccatc ggccccgccg
     4261 accgttgctg tgtgagaaca gactttccag gaggttcatg cccaccgtca atcaactcgt
     4321 ccgcaagggt cgcgctctcc aaaaggggaa gacgaagacc cctgctctcg gcggagcgcc
     4381 gcagaagcgc ggcgtctgca cgcgcgtgtt cacgcacacc ccgaagaagc cgaactcggc
     4441 gcttcggaag gtcgcgcgcg tccgcctgac gaaccagatg gaggtcacga cctacatccc
     4501 gggcgagggc cacaatctcc aggagcaccc cgtcgttctc gttcgaggcg gccgcgtcaa
     4561 ggacctgccg ggcgtccggt acaaggtcat ccgcgccgcg ctcgacaccg ccggtgtcag
     4621 cgaccgcaag cagggccgct ccaagtacgg ggccaagaag tcgtaatgcc tcgtcgcgcc
     4681 gaaatccagg ttcgcgaagt cactccggac cccgtctaca actcgcagc
//
LOCUS       DASVEU010000220         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_346425,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000220 DASVEU010000000
VERSION     DASVEU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346425"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF225_09565"
     CDS             <1..188
                     /locus_tag="VF225_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145539.1"
                     /translation="GGAIRKQRRGEPLTSEEREALGRWQRLSNWRKALAIGGFAIGTF
                     GLGFTLGGLIFGRWRKA"
     gene            complement(432..728)
                     /locus_tag="VF225_09570"
     CDS             complement(432..728)
                     /locus_tag="VF225_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145540.1"
                     /translation="MTESPIFIDAALKMAPDPERVIESVPEAKALDAIAVGVAVKFAK
                     VPPTAATAITETAAKLRRIFDRGRFLSIERVSPFGLVDTLTIGSPGMGTGATSE"
     gene            706..1041
                     /locus_tag="VF225_09575"
     CDS             706..1041
                     /locus_tag="VF225_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145541.1"
                     /translation="MKIGDSVTGTVTITNTGTLAGVYSLSGSTTGSAPLASQLNLKVY
                     KDVDGGTALYDGSLGAISSAALGTFAAGDAHTFYFHVTFPTTGTDAGDNVLQGLAVST
                     TFTWSATQA"
     gene            1050..1571
                     /locus_tag="VF225_09580"
     CDS             1050..1571
                     /locus_tag="VF225_09580"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7145542.1"
                     /translation="MRRFIKTASKVTVVLVSGATVAFAVAYVALTVSGYKPVAVYSGS
                     MEPRIPIGGLALDRAIPSTNVRVGDVITFSDPYVKGRLVTHRVIDILETPHGLAYRTK
                     GDANPGRDPWTIRLADQVGRVSFTVPVAGYILFYAHTREVRGLLICLAGLSLLVGTLR
                     WIWRKEATAPLGA"
     gene            1576..>2363
                     /locus_tag="VF225_09585"
     CDS             1576..>2363
                     /locus_tag="VF225_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145543.1"
                     /translation="MRKLIVTACLSLAAAGLFTQLSSAAFTGSLAAPSNGITADALHN
                     YFSVSPGTDVQPGTSTAVVSGNVDGLSIDLGTVPSARTFSNVFRITNVSSATRTATLN
                     LSSVPQVASAVFASSGNTSVALPAGASTTLAVTTSTTVAGRGVGTFRLGLSGTSWLYR
                     DYSFHVDEAPEKPGAPTGTQKPAGRIDLSWGASTTTTNLAGYDVYRASGGGAYTKLTA
                     TPQLALTYSDTATADGTAYTYKINAVSSGTPVLSSLDSTTVTVTA"
BASE COUNT          404 a          839 c          726 g          394 t
ORIGIN      
        1 cgggcggcgc gatccggaag caacgacgag gggagccttt gacgtccgag gagcgcgagg
       61 cgctcggccg ctggcagcga ctctcgaact ggcgcaaagc gctcgcgatc ggcggattcg
      121 ccataggaac cttcggtctg ggcttcaccc taggcggcct cattttcggc cgctggcgca
      181 aagcctgact cccgcgcgtc cagcagagcc accagggccg gaggctaaga ccaggcgcag
      241 ccgggcttag ccgcaggcga actgatgcgg gcgcggacgc ggagcgcccg tgcccggacg
      301 gaagggaaca accgccaagc gaccacaccg tgagagtgcg cgggcttcgc ccgcgcacgt
      361 tatgcgcgaa gcgcagtagc taaagcccgg gcaaaagcgc agcttttgcc cggaatcggt
      421 tcctaccgaa ttcattccga cgtagccccc gtccccattc cgggggagcc gatggtgagc
      481 gtgtcaacca aaccgaaagg actgacacgc tcaatgctca agaacctccc tctgtcgaag
      541 atcctcctca gcttggcggc cgtttctgtg attgccgtcg cggcggtggg gggaaccttc
      601 gcaaacttca cagcaacgcc gactgcgatc gcgtccaacg ccttcgcctc gggcacgctc
      661 tcgatgaccc gctctgggtc cggtgccatc ttcaacgccg cgtcgatgaa gatcggcgac
      721 tcggtcaccg gcacggtcac gatcacgaac accggaactc tcgccggcgt gtattcgctg
      781 tccggctcga cgaccggtag tgcgcctctc gcaagccagc tcaacctgaa ggtgtacaag
      841 gacgtcgacg gcggcaccgc tctctacgac ggctcgctgg gcgcaatcag ctcagccgcc
      901 ctcgggacgt ttgcagctgg agatgcccac acgttctact tccacgtcac cttcccgacg
      961 accgggacgg atgcgggcga caacgtcctt cagggccttg ctgtcagcac gacgttcacc
     1021 tggtctgcga ctcaggcgta ggacgaacgg tgaggcgttt catcaagacg gcgagcaagg
     1081 tgacggtggt cctcgtttcg ggggcaaccg tcgccttcgc cgtcgcatac gttgcgctca
     1141 cggtctccgg ctacaagccc gtggccgtct actccggctc gatggagccc cgcatcccca
     1201 tcggcggtct cgcgctcgat cgcgcgattc cttcgacgaa cgtacgagtc ggcgacgtga
     1261 tcacgttctc cgatccctac gtcaaagggc gcctcgtcac gcatcgcgtg atcgacattc
     1321 tcgagactcc ccacgggctc gcatatcgca cgaagggcga tgcgaatccc gggcgcgacc
     1381 cctggacgat tcgactggcg gaccaggtcg gccgcgtctc gttcaccgtg ccggtcgccg
     1441 gctacatcct gttctacgcc cacacccgcg aggtgcgcgg cctcctcatc tgcctggcgg
     1501 gtctctcgct gctggtgggg acactgcggt ggatctggcg caaggaggcg accgcgcccc
     1561 tcggcgcgta actccatgcg caagctcatc gtcaccgcct gtctctcact cgctgccgcg
     1621 ggcctcttca cgcagctcag ctcggcagcg ttcaccggct cgctggcggc gccgtcgaac
     1681 ggcatcacgg ccgacgcgct acacaactac ttttcggtct cgcccggaac cgacgtacag
     1741 ccgggcacat cgaccgcggt tgtctcaggg aacgtcgacg ggctttcgat cgacctcggc
     1801 accgttccgt cggccagaac cttctccaac gtcttccgga tcacgaacgt cagcagcgcg
     1861 acgcgaaccg cgacgctgaa cctctcgtcg gtcccgcagg tggcgtccgc cgtcttcgcc
     1921 tcgagcggga acacttcggt cgcgctcccc gcgggcgcgt ccacgaccct ggccgtcacc
     1981 acttcgacga ccgtcgcggg tcgaggagtg ggcacgttcc gcctcggcct ctccggaacg
     2041 agctggctct accgcgacta ttcgtttcac gtcgacgagg cgccggagaa acccggcgct
     2101 ccgacgggaa cgcagaagcc ggctggtcgc atcgacctct cctggggcgc gtcgaccacg
     2161 acgacgaatc tcgccggcta cgacgtctat cgcgcgagcg gcggcggcgc ctacaccaag
     2221 ctcacggcga ctcctcaact cgcgttgacg tacagcgaca ccgcgacggc ggacggaacg
     2281 gcgtacacct acaagatcaa cgccgtctcg agcgggacgc cggtgctctc gagcctcgat
     2341 agcacgaccg tcactgtcac tgc
//
LOCUS       DASVEU010000221         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_734426,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000221 DASVEU010000000
VERSION     DASVEU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734426"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2763)
                     /locus_tag="VF225_09590"
     CDS             complement(<1..2763)
                     /locus_tag="VF225_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7145544.1"
                     /translation="MSQAEQVLWLNAVPLLVVAAASLGAAARLGSSGKRGRSQLEPAL
                     LAVFSAVGLTTAVYGIVLAVERKPPPGGTWLALALAALLVLPAVLALRAAARPAGSAG
                     EREREAGAPTAGAVGPSDDVDAVAELAGDSALALDRARSTAGLAEALERERFVARISA
                     RVRSELDIDELLRVAVEETGLAQGVDRCLIRLGSHGEIPTAQWQSAGLSPVKTGRHLA
                     VSNLAVKRRQTVAVEDVGTAPELEDASLGGRDALLSLGSRAVLAVPIVIFDELIGVLA
                     LHRNTPGRWDDEEIALTEAVAREIGLAVRVAQLLRENEQRMTQQGSLFRIAALLGQSL
                     SLSDTLEALAQAANEALGGSFAAVLMPRRGNLELAAAAGLPPLLAEALEGLVAGSATC
                     LQIAAEERRALASPDVTRDDRFEPLWVEQANAAGYRALLAVPAASPRAEQSGLVVVFF
                     ADERVFTDEDLELARHLGDAARGALERSELFEAERTARALSQQLARTGGVLATELDPA
                     AVLEEVVQQAPALLGADACAVRTLEDDELVVSAASGDGAEEAVGTRTSATGRLSGDVF
                     QSRSPLAVEDVTTDERLAGADAILLAGYAAFLGVPLSGPEGSLAGVLSVYATKPREWR
                     PEEVEALLALAGNTSAALANAELYSRVSLEKERSVAILANIADGIVAVDRDGQVVLWN
                     SAAEEITGVPQEEAIGRTTVQILQRQLESAETGTPYGQRLVSITRGAEEVWLSLSEAV
                     MRDPLGAVAGRIFAFRDISADRMVEQVKSDFVAAVSHELRTPLTSIYGFAETLLREDV
                     PFGEEERRIFLGYIASESERLTEIVDQLLNVARLDAGDLQVELGRIDVGTVVSELVET
                     VEESGVVNGHRFEIDLPDEPLAAEADPDKVRQVFNILVENALRYSPVGGTVTVGARRN
                     HDRVEVR"
     gene            3134..>3579
                     /locus_tag="VF225_09595"
     CDS             3134..>3579
                     /locus_tag="VF225_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145545.1"
                     /translation="METVERALSLYGAPVYVRKQIVHNAHVVRELEGRGAVFVEDEAE
                     APPGAILVLSAHGVAPSVYERAAVRGQTTIDATCPLVTKVHVQARRYAAEGRRIVLIG
                     HAGHEEVVGTLGEAPEATVLVESVEDAEALELPLGTPMAYITQTTLS"
BASE COUNT          583 a         1241 c         1219 g          536 t
ORIGIN      
        1 acgcacctcg actcggtcgt ggttgcgccg cgccccaacc gtgacggtgc cgccaaccgg
       61 cgagtagcgg agcgcgttct cgacgaggat gttgaagacc tgacgcacct tgtcgggatc
      121 tgcttcggcc gcgagcggct catcgggcag atcgatctcg aagcgatggc cgttcacgac
      181 gccgctctct tcgacggttt ccaccagttc cgagacgacg gtaccgacgt cgatccgtcc
      241 cagctcgacc tgcaggtcgc ccgcatcgag ccgtgcgacg ttcagtagct ggtcgacgat
      301 ctccgtcagg cgctcggact ccgatgcgat gtagccgagg aagatgcgcc gctcttcctc
      361 gccgaacggg acgtcctctc gaagcagggt ctcggcgaaa ccatagatcg acgtcagcgg
      421 cgtacgcagc tcgtgggaga cggcggcaac gaagtcggac ttgacctgct cgaccatgcg
      481 atcggcggag atgtcgcgga aggcgaagat gcggccggcg accgccccga gcgggtcgcg
      541 catcactgct tcgctcagcg agagccagac ttcctctgcg ccccgcgtga tcgagactag
      601 gcgctggccg taaggcgtcc ccgtctccgc agactcgagc tgtcgttgca ggatctggac
      661 cgtcgtccgg ccgatcgcct cctcctgagg tacgccggtg atctcttcgg cagccgagtt
      721 ccaaaggacg acctggccgt cgcggtcgac cgccacgatg ccgtcggcga tgttcgcaag
      781 gatcgcgacg ctccgctcct tctccaacga gacgcgcgag tagagctccg cgttcgcgag
      841 cgcggccgac gtgtttcctg ccagtgcgag cagcgcctcg acctcctcgg gccgccactc
      901 tcgaggtttg gtggcataga cggagagcac gccggccaga gagccttcgg gaccggagag
      961 gggcacgccc agaaacgctg cgtaaccggc caggaggatc gcatcggcac ccgccagccg
     1021 ttcgtcagtg gtgacgtctt cgaccgcgag cggcgaacgc gactggaaga catcgcctga
     1081 caaccgtccc gtcgcagacg ttcgagtccc gaccgcttcc tcggcaccat cgcccgaggc
     1141 ggcgctgacc acgagctcgt cgtcctcgag cgtgcgaact gcgcaagcgt cggccccgag
     1201 gagggcgggc gcctgctgca cgacttcctc cagaaccgcc gccgggtcga gctcggtggc
     1261 aaggacgccg cccgtccgcg cgagttgctg tgagagcgca cgcgcggtgc gctcggcttc
     1321 gaacagctcg cttcgttcga gcgcgccgcg cgccgcgtcg ccgagatggc gcgcgagctc
     1381 gaggtcctcg tcagtgaaga cgcgctcgtc cgcgaagaag acgacgacca ggcctgactg
     1441 ctccgcccgc gggcttgcgg caggcaccgc cagcaacgcg cggtatcccg ctgcgtttgc
     1501 ctgttcgacc cagaggggtt cgaagcggtc atcgcgcgtg acgtcgggag aggccagcgc
     1561 ccgcctctcc tcggcggcaa tctggaggca ggtcgccgac cccgccacca gcccttcgag
     1621 cgcctccgcg agcaagggtg gaaggccggc tgccgcggcg agctcgagat ttccgcgccg
     1681 tggcatcaga acggcagcga acgagccccc gagagcttcg ttcgcggcct gggcgagagc
     1741 ctcgagcgtg tccgagagcg acagcgactg accgaggagc gcagcgatcc gaaacagcga
     1801 tccctgctgg gtcatccgct gctcgttctc gcggagcagc tgcgccaccc gaacggcgag
     1861 cccaatctcg cgagccacgg cctcggtcag cgcgatctcc tcgtcgtccc atcgtccggg
     1921 tgtgttgcgg tggagcgcga ggactccgat cagctcgtcg aagatcacga tcggaaccgc
     1981 aagaacggcg cgcgagccga ggctcagcag cgcgtcgcgg ccaccgagcg aggcatcctc
     2041 gagctccggc gccgtcccga catcctcgac ggccacggtc tgacggcgtt tcaccgcgag
     2101 gttcgagacg gcgaggtgcc gtccggtctt caccggagac aagccggcgc tttgccactg
     2161 cgccgttggg atctcaccgt gcgagccgag ccggatcaga caccggtcga cgccctgggc
     2221 aagcccggtc tcctctactg ccacgcgcag cagctcgtcg atgtcgagct cggagcgaac
     2281 gcgtgccgag atccgggcga cgaagcgctc gcgttcgagc gcctcggcca gaccggcagt
     2341 cgagcgcgcc cgatcgagcg cgagggccga gtcgccggcg agttcggcaa ccgcgtcgac
     2401 atcgtcgctc ggaccgacag caccggctgt aggtgctccc gcctcgcgct cgcgctcgcc
     2461 ggcagagccc gcaggccgag ccgccgccct gagagccagg acggcgggca aaaccaggag
     2521 cgcagcgagc gccaaggcga gccaggtgcc gccgggcggg ggtttgcgct cgaccgccag
     2581 cacgatcccg tagacggcag tcgtgagccc gaccgccgaa aagaccgcga gcagcgccgg
     2641 ctcgagctga gagcgcccgc gctttcccga ggagccgagc cgcgcagcgg ccccgaggga
     2701 ggctgcagcc acgacgagca gcggcaccgc gttgagccac agaacttgtt cggcttgtga
     2761 cactgggcaa ttctgattgt agggagcggc cctggagcag tcgagcggcc gcaaggcgcc
     2821 tgagtaccac gctttccgcc agacgagccg ctttcctctc gcttccaaag atgggccgtt
     2881 cggcctattg gaaaggtccg taggacccgt tagcgtcaat cccactgggc gatgcgaacc
     2941 gcggtgcgtt cctggccttg cagtccgcgg tgagcactag gaggaggccc gtcgccaccg
     3001 acccccccga gcgacgggcc ttttcctgtt cgaacggcct cgagcggggg gcactgctac
     3061 cgtggccgcc ggtttggttc agcgggtctt gcttgcatcg ccccggggat attgcgccgg
     3121 agttgagcgc gccgtggaaa cggtcgagcg tgcgctctct ctttacggcg cgcccgtcta
     3181 cgtgcgcaag cagatcgtcc acaacgcgca cgtggtgcgc gaactggagg ggcgcggagc
     3241 ggtgttcgtc gaggacgagg cggaagctcc gcccggagcg atcctggtcc tctcggcgca
     3301 cggtgtcgcc ccgtcggtct acgagcgcgc cgccgtccgc ggacagacca cgatcgatgc
     3361 cacctgcccc ctcgtgacga aggtacacgt gcaggcgcga cgctatgcgg cggaaggacg
     3421 gcgcatcgtc ctgatcggac acgccggcca tgaagaggtc gtgggtactt tgggcgaagc
     3481 cccggaggcg acggtgctcg tcgagagcgt cgaggatgca gaagcgctcg agcttcccct
     3541 tggcaccccc atggcttaca tcacgcagac cacgctctc
//
LOCUS       DASVEU010000222         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1707979,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000222 DASVEU010000000
VERSION     DASVEU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707979"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF225_09600"
     CDS             <1..323
                     /locus_tag="VF225_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145546.1"
                     /translation="LPYEPAMADYAGNVDVSAKPHQQSLRRPPTAGLRDWRAQMSPED
                     IGAFDKVAGDLLAELGYETHERPDVAGRFKRASYNARAAAWRAAGLGLRRSPLWRRRH
                     PPLR"
     gene            complement(276..1625)
                     /locus_tag="VF225_09605"
     CDS             complement(276..1625)
                     /locus_tag="VF225_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7145547.1"
                     /translation="MRRRILLVVAVLVLLVAGAGVAYFLKSRERPGGKLDTKLSGVTA
                     SVATNVAVPTQPKKKKGRYHLVDDKRCWDNFGGDPARTLSRPGLDIGLPLRHFWVVGL
                     GSYIEYPPSYCSGILYVNTFGGRTVALDSHNGHRIWQRFGAKKPSTPAIAGPRLIVSS
                     HDGNLTAYNRFNGQLLWRLHVNAKIESSPVAIGRVVYFGATDGRVFAVYVRTGRVKWA
                     YDTGGRINSSPSIAGNRLFVSTYAGSIFSLRLSDGHKLWDRYFRRDVLRYESFYASPS
                     TDGRRVFTIARSGKIYAVSASSGRLIWTARVGGLGYSTPAIASGRVFIGGFDGGLRAF
                     RASNGQVLWRRNLGGKILGGSFVAGNLVFTSTLAARTFALRASDGKILWRLNIGKYSP
                     GIVTERHYFFTLNGIVMAWHGRRSPQILALQRAKKRQAAQKKSAQGRVTTAPKGRTP"
     gene            complement(1635..3176)
                     /locus_tag="VF225_09610"
     CDS             complement(1635..3176)
                     /locus_tag="VF225_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7145548.1"
                     /translation="MPHGDLAQLSAVIGGLGAAAMLLSHTRAQLLAGMAVTAVGEATL
                     AAALIPGADLKLLVSPASHAAALIVGVLLVGASAVALARYPAVVPIALLAVAPFRISV
                     SIGSQKAYLLVPLYVVLGAAFLALVFRALRRDVGKPLPPLLAVPAGTFVALAGLSLLW
                     TRDLRQGTIELLFFLFPFSALVVAVARSPFRSWHPRALATTLVALAGLFSAVALFQRL
                     THGHLLAGDVARANAYTTYFRVTSLFKDPSIFGRHVVIAIAVLLVAVWLGRIGFWAGA
                     ALIAFLWAGLLFSYSQSSFVSLFAVAVAVSFALGGPRMRRVLLVGGVAFVLAGAAFVA
                     ATAVNDSARQATSGRTRLARVTWVVFTNHPLVGVGIGGQPQASKDEAKTQLSAKRDRS
                     HTTPLTVAAELGILGLLAYAALLAGATRLLLLLTRSNRALGLGAAAVFLALFVHSLFY
                     AGFFEDPIVWGVMALAAWAWVALPRESETPPSPRKPAQLTEPAQDGGPRRTAPELQHQ
                     APLAD"
     gene            3245..>3548
                     /locus_tag="VF225_09615"
     CDS             3245..>3548
                     /locus_tag="VF225_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7145549.1"
                     /translation="MIGAWKRVLVLAPHTDDGEFGAGGTMTRLVEEGAEVRYVAFSIA
                     TRSLPEGFPPDTLAQEVRAATAELGISAENLTVHDFDVRTFPQHRQDILELLVALWE"
BASE COUNT          645 a         1153 c         1258 g          492 t
ORIGIN      
        1 ggcttccgta cgagccagcc atggccgact acgcgggcaa cgtggacgtg tcggccaagc
       61 cgcaccaaca gagcctcaga cgccctccga ccgcggggct gcgagactgg cgcgcgcaga
      121 tgtctcccga ggacatcggc gccttcgaca aggtcgcggg cgatctcctc gccgaactcg
      181 ggtacgagac gcacgagcgg cccgacgtgg ccggaaggtt caagcgagcg tcgtacaacg
      241 cgcgggccgc ggcgtggcgg gcggccggcc tcggtctacg gcgttcgccc ctttggcgcc
      301 gtcgtcaccc gcccctgcgc tgacttcttc tgcgccgcct gccgcttctt ggcgcgctga
      361 agggcgagga tctgcgggct tcgtcgtccg tgccacgcca tcacgattcc gttcagcgtg
      421 aagaagtaat gccgctcggt gacgattccc ggcgaatact tgccgatgtt caggcgccag
      481 aggatcttgc cgtccgaggc gcgcagcgcg aacgtgcgcg ccgcgagcgt cgaggtgaag
      541 acgaggttcc cggccacgaa cgagccgccg agaatcttgc cgccgaggtt gcggcgccac
      601 agcacctgtc cgttgctcgc gcgaaatgcg cgcaggcctc cgtcgaaccc gccgatgaat
      661 acgcggccgc tggcgatcgc cggagtggaa tagccgagtc caccgacccg cgcggtccag
      721 atcaagcgcc cgctgctcgc ggaaactgcg tagatctttc cggagcgggc gatggtgaag
      781 acgcgccgcc cgtcggtcga cgggctcgcg tagaagctct cgtagcgaag cacgtcgcgg
      841 cgaaagtatc tgtcccagag cttgtgcccg tcgctgaggc gcaaggagaa gatcgaacct
      901 gcgtatgtcg agacgaagag gcggttgccg gcgatcgacg gactggagtt gattcgaccg
      961 cccgtgtcgt acgcccactt cactcgaccg gtgcgcacgt acacggcgaa gactcgtccg
     1021 tccgttgcgc cgaagtagac gacgcgcccg atcgcgaccg gcgacgactc gatcttggcg
     1081 ttgacgtgga gccgccagag cagctggccg ttgaaacggt tgtacgcggt cagattcccg
     1141 tcgtgcgagc tgacgatcag ccgcgggccg gcgatcgccg gcgtcgaggg cttcttggcc
     1201 ccgaagcgct gccagatcct gtgcccgttg tgcgagtcga gggcgacggt gcggccaccg
     1261 aaggtgttca cgtacaggat tccgctgcag tagctgggcg ggtactcgat atagctgccc
     1321 aggccgacca cccagaagtg ccgtagtggg aggccgatgt cgaggccggg tcgtgacagg
     1381 gtccgggcgg gatcgccgcc gaagttgtcc cagcagcgtt tgtcgtcgac taggtgatag
     1441 cgacctttct tcttcttcgg ctgcgtcgga acggccacgt tcgtcgcgac ggacgccgtg
     1501 actccgctca gcttcgtgtc gagctttccg cccggccgct cacgcgactt gaggaagtag
     1561 gccacccccg cgcccgcgac gagcagcaca aggacagcga cgacgagaag gatccgacgg
     1621 cgcaccgggc gaaactagtc tgccagcggg gcctgatgtt ggagctcggg agccgttcgc
     1681 cgcggtccac cgtcctgtgc aggctcggtg agctgcgccg gtttgcgcgg gcttggcggg
     1741 gtctcgctct cacgcgggag agcaacccag gcccaggcag cgagcgccat cacgccccac
     1801 acgatcgggt cctcgaagaa gcccgcgtag aagagcgagt gcacgaacag ggcgaggaag
     1861 accgcggctg ctcctaatcc cagcgcgcga ttcgagcgcg tcagcaggag cagcagccgc
     1921 gtcgctccgg caagcagtgc tgcataggcg aggaggccga ggatgccgag ctctgccgcg
     1981 accgtgagcg gcgtggtgtg cgagcggtca cgcttggcgg acagctgtgt cttcgcttcg
     2041 tccttgcttg cctgcggctg gccaccgatc ccgacgccga cgagaggatg attcgtgaag
     2101 acgacccagg tcacccgggc gagccgcgtc cggccactcg tggcctggcg ggccgagtcg
     2161 ttcactgccg tagcggcgac gaacgccgct ccggcgagga cgaacgcgac accgcccacg
     2221 agcaacacgc gccgcattct cggtcctccg agcgcgaacg acacagccac ggcgaccgcg
     2281 aagagtgaga cgaagctcga ctgcgagtac gagaagagca gccctgccca cagaaacgcg
     2341 atcagggcag ctccggccca gaagccaatt cggccgagcc agaccgcgac gagaagaacg
     2401 gcgatcgcga tcacgacgtg ccgcccgaag atgctcggat ccttgaagag cgacgtgacg
     2461 cggaagtacg tcgtatacgc attcgcacgc gcgacgtccc cggcgaggag gtgcccgtgc
     2521 gtcagacgtt gaaagagcgc aaccgccgag aacagcccgg ccagcgcgac gagtgtcgtc
     2581 gcgagcgcgc gcggatgcca cgagcggaag ggcgaccgcg caacggcgac gacgagcgcg
     2641 gagaacggaa agaggaagaa gaggagctcg atggtgccct ggcgcaggtc gcgcgtccag
     2701 agcagcgaga gcccggcgag ggccacgaac gttccggccg gcacagcgag cagcggcggc
     2761 agcggtttcc ctacatcgcg ccgcagtgcg cgaaacacga gggcgaggaa ggcggccccc
     2821 aggaccacgt acagcggcac gagcaggtag gccttctgcg agccgatcga gacggagatc
     2881 cgaaacgggg ccacggcgag gagagcgatc gggacgacgg cgggatagcg agcgagcgcc
     2941 actgcgcttg cgccgacgag gagaacgcca acaatgagtg cggcggcgtg gctcgcagga
     3001 ctgacgagca gcttgagatc cgcccccggt atgagggccg ccgcgagggt cgcctcgccg
     3061 accgcggtca cggccattcc tgccaggagc tgagcgcgcg tgtgcgaaag gagcatcgcc
     3121 gccgccccca gcccgccgat taccgcagac agctgagcca ggtcgccatg gggcatgaag
     3181 gagaaagact acggagacgg caccgcgagc ggtgccggag gtggatcgtg ataggaaggg
     3241 ccgcgtgatc ggagcctgga agcgcgtcct cgtgctcgcc cctcataccg acgacggcga
     3301 gttcggcgcc ggcgggacga tgacacgcct ggtggaggag ggggcggaag ttcgctacgt
     3361 ggcgttctcg atcgccacgc ggtcgctgcc ggaaggcttc ccaccggaca cgctggccca
     3421 ggaggtgagg gcagccaccg ccgagctcgg gatctcggcc gagaacctga ccgttcatga
     3481 cttcgacgtg cggacgtttc cccagcatcg gcaggacatc ctcgagctgc tcgtcgcgct
     3541 ctgggagg
//
LOCUS       DASVEU010000223         7136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_155493,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000223 DASVEU010000000
VERSION     DASVEU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155493"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VF225_09620"
     CDS             <1..1526
                     /locus_tag="VF225_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145550.1"
                     /translation="ENLEMFGRLFRLSRAEARRRAAELLERFELADAADRTAKTYSGG
                     MRRRLDLASSLLTQPRVLFLDEPTTGLDPRSRNEIWAIVRDLRREGTTILLTTQYLEE
                     ADQLADEIAVIDHGRVIAEGTGNELKDRVGGQILEVELTDVGQRDEAEAALLRVGCGA
                     PEKDERPNRLTLPAPRESLVLVEEAAAELRRAGIGVSDMGLRRPTLDDVFLQLTGEAP
                     SENGKPPIAAAVTDEKVLGRPRRFPRPHAPSLPAAKTSLTDTAVVTMRNLRHFWRQPD
                     LLVFSTIQPIMFVVLFVYVFGGAVGLALPPGVTYVDYLLPGIFVQSVTFRASNTAVGL
                     SEDLRRGVIDRFRSMPMARSAVLLGRTTADLVRNILIIGLMIGVGYLVGFRFQAGVIP
                     ALACVALVAAFGFALSWIFAFVSLVVRGAETAQTAGFVLLFPLVFASSVFVPVSSMPD
                     WLQPIAKASPVTLTADAARTLALTGGVPNSLWGSLAWIVGILALFVPLSVWRYRRMS"
     gene            1532..2512
                     /locus_tag="VF225_09625"
     CDS             1532..2512
                     /locus_tag="VF225_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145551.1"
                     /translation="MVRLYSATGDGFARLDEAGEAWTVELSLAGSGAQCLAVDAADPD
                     TVFVGLREGGVRRSVDGGRTWVDCKLPEPGVFSLAVSAADGAVYAGTEPSRLFRSGDK
                     GESWRELKALLELPSRPHWSFPPRPWTSHVRWIAPSPHDADLLLVGIELGGLMRSSDG
                     GQSWQDHRPGAQRDVHSVAWHPRARGRAYEAGGGGAAFSTDAGETWQPADEGRDRHYT
                     WSVTVDPDDADRWYVSASTGPYAAHGRGDPQARIYRRRDGEPWQPLAGGLPEPLPAMP
                     YVLLSTDGRLFAGLADGQLWESLDQGDSWTPLQLRGGALDALVALAFATQ"
     gene            complement(2632..4395)
                     /locus_tag="VF225_09630"
     CDS             complement(2632..4395)
                     /locus_tag="VF225_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7145552.1"
                     /translation="MAAPLAPYVDALPLPSRLIASKHDGRLTVRIRAAAHRFHRDLPD
                     SSIWGFEGIIPGPTIEAERGQPITVEWRNELEGAFPVSTTVAPHPTDADGVPVQCLPG
                     LSGGEPDRHASRLIGHTVVHLHGGLTPASYDGWAENLFAPGQPAVYHYPMDQRAALLW
                     YHDHVMGITKLDVYAGLAGLWIVRDDRERDLGLPEGPPFEVPLLIQDRNFDVGEQGRL
                     SGRLVHKTDPDVMESFAPFTAVNGKVWPVLDVQPTTYRFRVLNGSNARTYRLVLVHDG
                     EPALERIAQIGTDHGLLRAPATVPRDGLVLASAERADLLVDFSDLAPGNQLTVLNTAA
                     APFDGSRSPAADAEDAVNLEGLLPYPQVLRFRVVGGAPSRRSIPRELATDFVAPAAEA
                     LAGVRRRAIALVERELDDEPNMLTMGELAVTADADRGPWLSVIDGGETTRYRVVAAQF
                     EDTTTFFPMLGEYEVWQLINLTGDTHPIHLHLDPFQILARRPIRYEIPDGGIGERDLA
                     ATVTVERDPDDELDHAIDENERGLKDTVRVNPNEIVEIAVCFTTYSGRYMYHCHILEH
                     EDRDMMRPFVTMPPELMPFMA"
     gene            4417..4716
                     /locus_tag="VF225_09635"
     CDS             4417..4716
                     /locus_tag="VF225_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145553.1"
                     /translation="MNADGAAFGACSTRAICAQASHSGVRDARTPQTIGRFTDASVSR
                     LDDGEPMSENRRAKKLIGERSAVGREAPIVVTPSGLARLVAQTAALIRRRASRTR"
     gene            complement(4717..5163)
                     /locus_tag="VF225_09640"
     CDS             complement(4717..5163)
                     /locus_tag="VF225_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7145554.1"
                     /translation="MEQSALLKNVSHLSLERNPVLVEHAKQRAESVQNRIADRITAFS
                     GSMWFVYIHIIWFASWIGFGVERYPYGLLTMIVSLEAIFLSTFVMISQNRADAKRQAI
                     ADQQWQTVKEEDKQNEELLKLSNQILELTRAIHAVTSTRSATTPAK"
     gene            complement(5230..5880)
                     /locus_tag="VF225_09645"
     CDS             complement(5230..5880)
                     /locus_tag="VF225_09645"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7145555.1"
                     /translation="MSNNDLARHVTDELYWDPKVDSAAIAVSAADGVVTLRGTVGSFR
                     EKQEAKHDAERIYGVKDVKNEIEVRILNEHRRSDAELRGAVLQAMMLDNLVPSTIDAK
                     VDNGVVTLTGKANWQHQRDEAESVAGNLLGVMEVEDEIELIAPGPTAHDVEHSIKKAM
                     ERNAKLDAESVDVESSNGTITLRGTVSSWADHDEAVGAAWAAPGVTEVKDHILVAY"
     gene            complement(6012..6722)
                     /locus_tag="VF225_09650"
     CDS             complement(6012..6722)
                     /locus_tag="VF225_09650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7145556.1"
                     /translation="MLPLSDGMPARRFPIVNVAIIVANFAVWLFYELPNLQSAVFHAS
                     FYPCTVTHACHGPEPWGVSWITAMFLHGSWDHILGNMLFLAVFGKNVEDAFGHLRYLA
                     FYFAGGFVAMMTQTLMTLAFSNAADGRVPNLGASGAIAAVLGAYFVLYPNSTVLGLFV
                     IFPVRISAWFFLGFWFLYQLFEANFGLFGATANGGGVAFFAHVGGFIFGVAVAAFLSS
                     LGRVAPQDGRQALLRAST"
     gene            complement(6803..>7136)
                     /locus_tag="VF225_09655"
     CDS             complement(6803..>7136)
                     /locus_tag="VF225_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7145557.1"
                     /translation="PLIGAILVVAVGLPQSVHVAALALIWLVAVREFQSYVVNPHLMG
                     KSVGLSPLVTLVSVSVVGLLFGGFAVILAIPVTSAVATMIDVFVLDHEPPPEAPSRQR
                     RALPSRAQ"
BASE COUNT         1146 a         2347 c         2431 g         1212 t
ORIGIN      
        1 gcgagaacct ggagatgttc gggcgcctgt tccggctctc tcgagcagag gctcgccgtc
       61 gcgcggcgga gctgctggag cgcttcgagc tcgccgacgc cgccgaccga accgcaaaga
      121 cgtactcggg cgggatgcga cggcggctcg acctggcgtc cagcctgctg actcagccgc
      181 gtgtgctctt cctggacgag ccgacgacgg gtctcgatcc tcggagccgc aacgagatct
      241 gggcgatcgt ccgtgacctg cgccgggagg ggacgacgat cctgttgacg acgcagtatc
      301 tcgaggaggc ggatcagctc gcggacgaga tcgccgtgat tgaccacggc cgcgtgatcg
      361 cggaagggac cggcaacgag ctcaaagacc gtgtcggcgg ccagatcctc gaggtcgagc
      421 tgaccgacgt ggggcagcgt gacgaggccg aggccgcgct ccttcgggtc ggctgcggcg
      481 cgccggagaa ggacgagcgg ccgaaccggc tgaccctgcc ggcgccgcgg gagagcctcg
      541 tcctcgtcga ggaggccgcg gccgagctac gtcgcgccgg gatcggcgtc agcgacatgg
      601 gcctgcgcag accgacgctc gacgacgtct tcctccagct gaccggcgag gcgccgagcg
      661 agaacggcaa accgccgatc gccgctgccg tgactgacga gaaggtcctg gggcgccctc
      721 gccggtttcc gcgcccgcac gcaccgtcgc tgccagccgc caagacgagc ctcaccgaca
      781 ccgccgtcgt gacgatgcgg aacctccgtc acttctggcg ccagcccgac ctgctcgtgt
      841 tctcgacgat ccagccgatc atgttcgttg ttctgttcgt ctacgtcttc ggcggtgccg
      901 tcggcctggc gctgccgccg ggagtcacct acgtcgacta cctcctgccc gggatcttcg
      961 tccagtcggt cacgttccgc gcctcgaaca cggctgtcgg cctctccgag gacctacgcc
     1021 gaggcgtcat cgaccgcttc cgctcgatgc cgatggcgcg ctcggcggtc ctgctcgggc
     1081 gaacgaccgc ggacctcgtg cgaaacatcc tgatcatcgg cctgatgatc ggcgtcggct
     1141 acctcgtcgg cttccgcttc caggcgggcg tcatcccggc gctcgcctgc gtcgccctcg
     1201 tcgccgcctt cgggttcgcg ctgagctgga tcttcgcctt cgtctctttg gtcgtccgag
     1261 gcgccgagac cgcgcagacg gccgggttcg tgctgctgtt tccactcgtc ttcgcgagct
     1321 cggtcttcgt cccggtctcg agcatgccgg actggctgca accgatcgcc aaagcctcgc
     1381 cggtgacgct gaccgcggac gcggcgcgca ccctcgcgct caccggcggt gtgcccaatt
     1441 cgctctgggg ttcgctcgcg tggatcgtgg gaatcctggc gctcttcgtg ccgctctcgg
     1501 tctggcgtta ccggaggatg agctaactca ggtggtgcgc ctctactccg caaccggcga
     1561 tggcttcgcc cgactcgacg aagcgggtga ggcgtggaca gtcgagctct ccttggctgg
     1621 aagcggcgcg caatgcctcg ccgtcgacgc ggccgatccc gacaccgtct tcgtcggtct
     1681 ccgcgagggc ggtgtgcgcc gcagcgtcga cggcggccga acctgggtcg actgcaagtt
     1741 gcctgagcct ggggtgttct cgcttgccgt cagcgctgct gatggcgcgg tgtacgcggg
     1801 caccgagccg agtcgactat tccgtagcgg cgacaagggt gagagctggc gcgagttgaa
     1861 agcactcctc gagctgccct cgcggccgca ctggagtttc ccgccgcggc cgtggacgtc
     1921 gcacgtccgc tggatcgccc cgagcccgca cgacgccgac ctcctactgg tcgggatcga
     1981 gctcggcggc ctgatgcgct ccagcgacgg cgggcagagc tggcaggacc accggcccgg
     2041 cgcgcaaagg gacgtgcact cggtcgcctg gcacccacgc gcccgaggac gggcgtacga
     2101 agccggcggc ggcggtgccg cgttcagcac cgacgccggc gagacctggc agccggccga
     2161 cgagggccgc gaccgccact acacgtggtc ggtcaccgtc gatcccgacg acgccgaccg
     2221 ctggtacgtc tcggcaagta ccggccccta cgcggcccac ggtcgcggcg acccgcaggc
     2281 acggatctac cggcgacgcg acggcgaacc ttggcagccg ctcgctggtg gcctccccga
     2341 gccgctaccg gcgatgccct acgtactcct ctctaccgat ggccgtctgt tcgccggtct
     2401 cgccgacggc caactctggg agagcctcga tcaaggcgac agctggacgc cgctccagct
     2461 ccgaggcggc gcgctcgacg ccctcgttgc cctcgccttc gccacgcagt gacgatcggg
     2521 atggagtagt cacgagatag ccactctcgt cgagatcgac ggaaagcgaa tcggccgccc
     2581 gcggatcgcg tgcagaaagc tcgcaggctt atgagccaac ccgtggaccc ctcaggccat
     2641 gaacggcatg agctccggcg gcatcgtgac gaagggtcgc atcatgtcgc ggtcttcgtg
     2701 ctcgaggatg tggcagtgat acatgtaccg gccgctgtag gtggtgaagc agacggcgat
     2761 ctcgacgatc tcgttgggat tgacgcgcac ggtgtccttc aatccccgtt cgttttcgtc
     2821 gatcgcgtgg tcgagctcat cgtctgggtc gcgctcgacc gtgacggtcg cggcgaggtc
     2881 gcgctcgccg atgccgccgt ccgggatctc gtagcggatc ggccggcgcg ccaagatctg
     2941 gaacgggtcg aggtggagat ggatcgggtg agtgtcgccg gtcaggttga tcagctgcca
     3001 gacctcgtac tcgccgagca ttgggaagaa cgtcgtcgtg tcctcgaact gggcggcgac
     3061 gacgcggtat cgggtggttt cgccgccgtc gatgacgctg agccacggcc cgcggtcggc
     3121 gtcggccgta accgccagct cccccatcgt cagcatgttc ggctcgtcgt cgagctcacg
     3181 ctcgaccagg gcgatcgcgc gccggcgcac gccagcgaga gcctccgccg ccggagccac
     3241 gaagtcggtc gcaagctcgc gtgggatcga gcggcggctc ggcgcgccgc ctacgacacg
     3301 gaagcgcagc acctgcggat aggggagtag accctcgagg ttcacggcat cctcggcgtc
     3361 tgcggccggg gagcgcgagc cgtcgaacgg tgcggcggct gtgttaagca ctgtcagctg
     3421 gtttccgggc gcgaggtcgg agaagtcgac gagtaggtct gcacgttcgg ctgatgcgag
     3481 cacgaggccg tcgcgaggca cggtcgcggg agcacggagc agcccgtggt cggtgccgat
     3541 ctgcgcgatc cgttccagcg ccggctcgcc atcgtgcacc aggacgagcc ggtaggtgcg
     3601 agcgttcgaa ccgttcaaca ctcggaagcg gtaggtcgtc ggctgcacgt cgagcaccgg
     3661 ccagaccttg ccgttcacgg cggtgaacgg cgcaaaggac tccatgacgt ccgggtcggt
     3721 cttgtggacg agccggccgc tgagcctccc ctgctctccg acgtcgaagt ttcggtcctg
     3781 aatcagtagc ggcacctcga acggtggtcc ttccggcagc ccgaggtcgc gctcccggtc
     3841 gtcacgcacg atccagagtc ccgcgagccc cgcgtagacg tcgagcttcg ttatgcccat
     3901 gacgtggtcg tggtaccaga gcagcgccgc gcgctggtcc atcgggtagt ggtagacggc
     3961 cggctggccc ggggcgaaga ggttctcggc ccagccgtcg taggaggcgg gtgtgaggcc
     4021 gccgtgtagg tggacgactg tgtggccgat caatctggac gcgtggcggt ccggctcgcc
     4081 cccgctcagg ccggggaggc actgcactgg gacaccgtcg gcgtcggtcg ggtgcggggc
     4141 gacggtagtg gacaccggga acgccccctc caactcgttc cgccactcga ccgtgattgg
     4201 ctgcccgcgc tcggcctcga tcgtcgggcc gggaatgatc ccttcgaacc cccagatgct
     4261 cgagtcggga aggtcgcggt ggaagcggtg cgcagcagcg cggatgcgga ccgtcagccg
     4321 gccgtcatgc ttcgacgcga tcaggcgtga cggcagcgga agggcatcga cgtacggagc
     4381 cagcggtgca gccaatgcgc gagtcatgat gagccgatga acgccgacgg cgcggctttc
     4441 ggggcgtgct caacgcgtgc catatgcgcg caagcctctc actccggtgt ccgcgatgcg
     4501 cggacgccgc aaacgatcgg ccggttcacc gatgcatccg taagccgcct cgacgatggt
     4561 gagccgatgt cagagaatcg ccgcgccaag aagctgatcg gcgaacgcag tgccgtcggc
     4621 cgggaagcgc cgatcgtcgt cactccatcg gggctcgcgc gtctggtcgc gcagaccgcc
     4681 gcgctcatcc gaaggagagc ttcccggacc cgatgatcac ttcgccggcg tcgtcgctga
     4741 cctggtgctc gtgactgcgt ggattgcccg tgtgagctcg aggatctggt tcgacagctt
     4801 gagcagctcc tcgttctgct tgtcctcttc cttcacggtc tgccactgct gatcggcgat
     4861 cgcctgacgc ttcgcatcgg cacggttctg gctgatcatg acgaaggtcg agaggaagat
     4921 cgcctcgagc gaaacgatca tcgtgaggag gccgtacgga tatctttcga ccccgaagcc
     4981 gatccatgac gcgaaccaga tgatgtggat gtagacgaac cacatcgatc cggagaacgc
     5041 ggtgatcctg tccgcgatcc ggttctgaac gctctccgct cgttgtttgg cgtgctcgac
     5101 gagcacggga tttcgctcga gcgagaggtg tgagacgttc ttcagcagag ccgattgctc
     5161 catcgccagc cgctcccttc cggctcgacg gcacggacct cgagtggccc gtgccgccgt
     5221 tagccgttct tagtacgcga cgagaatgtg atccttcacc tcggtgacgc caggcgccgc
     5281 ccacgcggca ccgacagcct cgtcgtggtc ggcccaggag ctgaccgtcc cacggagggt
     5341 gatcgtcccg ttggagctct ccacgtcaac gctctccgcg tccagcttgg cgtttcgctc
     5401 catcgctttc ttgatcgagt gctcgacgtc gtgcgcggtg ggtcccggcg cgatgagctc
     5461 gatctcgtcc tcgacttcca tgacgccgag aaggttgcct gcgacggatt cggcctcatc
     5521 gcgctggtgc tgccagttgg ccttccccgt cagcgtgacg acgccgttgt cgaccttcgc
     5581 gtcgatggtc gaggggacga ggttgtcgag catcatcgcc tgcaggaccg cgccgcgaag
     5641 ctcggcgtcg gaacgccggt gctcgttcaa gatgcgcacc tcgatctcgt tcttgacgtc
     5701 cttgaccccg tagatgcgct cggcatcgtg cttggcttcc tgcttctcgc ggaagctgcc
     5761 gaccgtgccg cgcagcgtca cgacaccgtc ggcggccgag acggcaatgg ccgcactgtc
     5821 gaccttcggg tcccagtaga gctcgtcggt gacatggcgt gccaagtcgt tgttggacat
     5881 ttgagtcctt tcgtcctccg gccgtgggct cgatgcgcac gaatcaccgg ttgtcacgat
     5941 catcgccgtc gatcggtcac agaacatcgg tgctgctgct gagcggttgc ggaaagttcg
     6001 ctgtcagccg ctcaggtcga agcgcgtagc agcgcctgtc ttccgtcctg cggcgcgacg
     6061 cgcccgaggc tgctgaggaa cgccgcgacg gcgacgccga agatgaagcc gccgacgtgg
     6121 gcgaagaatg cgacgccgcc gccgttggcc gttgcaccga agagcccgaa gttcgcctcg
     6181 aagagctggt agaggaacca gaagccgagg aagaaccacg ccgagatccg cactgggaag
     6241 atcacgaaca gccccaacac ggtggagttc ggatagagga cgaagtaggc gccgagaact
     6301 gccgcgatgg cgccgctcgc tccgaggttc ggcacgcgac cgtctgctgc gttactgaac
     6361 gccagtgtca tgagagtctg ggtcatcatc gccacgaagc cgccggcgaa gtagaaggcg
     6421 aggtaacgga gatgcccgaa ggcgtcctcg acgttcttgc cgaacaccgc gaggaacagc
     6481 atgttgccga ggatgtgatc ccaactcccg tgcaggaaca tcgccgtgat ccagctcact
     6541 ccccacggct cagggccatg acacgcgtgt gtgaccgtgc aggggtagaa ggatgcgtgg
     6601 aacacggccg attgcagatt cggtagctcg tagaacagcc acaccgcgaa gttggcgacg
     6661 atgatcgcaa cgttgacgat cgggaagcgc cgcgccggca ttccgtcgga gaggggaagc
     6721 atcgagatca gtctggtcgg cttgcgtttc cgtagcgtcg gcaaggtcgc tgacgcgttg
     6781 cgcgaaacag cgacggttca gatcattgcg cccgactggg aagtgcgcgg cgttgacgtg
     6841 atggcgcttc ggggggaggc tcgtggtcga gaacgaagac atcgatcatc gtcgcaaccg
     6901 ccgacgtgac gggaatcgcc aggatcaccg cgaacccgcc gaacagcagg cctacaacgg
     6961 acacggagac cagcgtcacg agcggtgaca gaccgaccga tttccccatc aggtgcgggt
     7021 tcacgacgta gctctgaaac tcccgtacgg caacgagcca gatcagggcc aatgcggcaa
     7081 cgtgcacgga ctgcgggaga ccgacggcga cgacgagaat ggcgccgatg agtggt
//
LOCUS       DASVEU010000224         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_739423,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000224 DASVEU010000000
VERSION     DASVEU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739423"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..452)
                     /gene="tsaB"
                     /locus_tag="VF225_09660"
     CDS             complement(69..452)
                     /gene="tsaB"
                     /locus_tag="VF225_09660"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX7145558.1"
                     /translation="MLILAFDTATDVATSALVSDGEVLGERTSRAVTVLEDIDALLRQ
                     AGAHTRELDGIAVGIGPGSFTGIRIGLATARGLALALGVPVAGVSTLDALAAGAPLAL
                     PVIDARRGEVFVLQGEPRVLVPAGL"
     gene            complement(421..855)
                     /gene="tsaE"
                     /locus_tag="VF225_09665"
     CDS             complement(421..855)
                     /gene="tsaE"
                     /locus_tag="VF225_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7145559.1"
                     /translation="MIETRSPEETEQVAASLAADLRPGDVVTVSGELGSGKTTFVRGA
                     CRALGVEGAVTSPTYTIGHRYKGRVDVSHLDLFRFRGVSPAEWGDLEPYFDDAIVFVE
                     WPEAGAGVLPAPRVEVELEYGEEGHRLVTIRGDADSGLRHGD"
     gene            complement(852..1511)
                     /locus_tag="VF225_09670"
     CDS             complement(852..1511)
                     /locus_tag="VF225_09670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7145560.1"
                     /translation="MAAVTELTRADELHAFGRLVADCTKCTLAAGRTQVVFGAGDPDS
                     DLMFVGEAPGFHEDKQGVPFVGAAGKLLEQLLAGIGLARDEVYIANVLKCRPPGNRDP
                     LPEEIQACESHLWRQIELIEPRVVATLGNFATKLLSGRPTGITRVHGQEQEVTLGGRR
                     VLLYPIYHPAAALYTPRMLEVLKSDFARLPELMGREVAPPEPVYVAPVVAEPAVQLGL
                     F"
     gene            complement(1495..1704)
                     /locus_tag="VF225_09675"
     CDS             complement(1495..1704)
                     /locus_tag="VF225_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7145561.1"
                     /translation="MPMYEYVCMKCEEHFEELVRGDQVVACPECGATNVARQLSTFSM
                     VGTAGQTGAFSSGTGCCGGSCGCGH"
     gene            complement(1750..3588)
                     /locus_tag="VF225_09680"
     CDS             complement(1750..3588)
                     /locus_tag="VF225_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7145562.1"
                     /translation="MGRRFLLGLAVVVVLAAPGQARAAIALSPCPETTNGVQCGTVDV
                     PLDRSGAVPGTIPLHVEVLPASGLPRGTMFLIAGGPGQGSAGAYGLGSTGSAQDLQNM
                     FPGYTLVAFDNRGTGQSGLINCPGLQNSTMTTIEQDAALARDCAAIIGPGRQFYATRD
                     HAEDMESVRVALGLGRIGLFGVSYGTKLALAYALAHPAVVERIALDSVVAPTFPDPFE
                     RNVLRAMPGTLAAFCAGGICNGATRDFAGDVVKLANRLEARPVKGKVIAPNGKLRALQ
                     MNGEELISMIIDADLSPGLAAEAPAAVHAALLGNARPLLRLYDLDLRTSKLSAEDLSF
                     GLYAATNCADGRFPFAPDTPPAARRAILDATIGAFPPGSVGPFGNWAARIGTAYFCEQ
                     WPTPTGNTPLGPGPLPNVPVLALNGGFDLRTPTANADAVISAFPKGQLITVPGVGHSV
                     SSADFSGCASDAIREWILGTLQNFRKASCPRVPPVGKILGAFPRRPAKATVKATLAAA
                     AKTVREAEATWLQIVLSSASFAPRGLYGGKLVLSQQALSFTLTRYSAVAGIQVTGKVS
                     FVPGGLPLAYKGTLRVSGSSAVRGTLTFSKDSVSGRLGGRVVKGSY"
     gene            3742..>4007
                     /locus_tag="VF225_09685"
     CDS             3742..>4007
                     /locus_tag="VF225_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145563.1"
                     /translation="MGDDRELVLLCVPDGSIAEVAAGLEEGPWVAHVSGARPLSALDP
                     HRRRFSIHPLQTFTHARGAEQLDGAWAAVTAESDEARVAGFWLA"
BASE COUNT          677 a         1356 c         1377 g          597 t
ORIGIN      
        1 ccggctgcct ccagcaggga ccggtaacgg acggcgccgc tgcccacgca gactgtgccg
       61 gcagtgagct acagcccagc ggggaccagg acacgaggtt ctccctggag cacgaagacc
      121 tcgccgcgcc gggcgtcgat caccggcaat gccagaggcg cgccggccgc cagcgcgtcg
      181 agcgtcgaca cgcccgccac cgggaccccg agggcgagtg cgaggccgcg ggcggtcgcg
      241 agcccgatcc ggatgccggt gaagctgccg ggcccgatcc cgacggcaat cccgtcgagc
      301 tcgcgggtgt gcgcgcctgc ctggcgcaac aaggcgtcga tgtcctcgag cacggtgacc
      361 gcgcgcgacg tccgctctcc cagtacctca ccgtccgaga cgagcgcgct cgtcgcgacg
      421 tcagtcgccg tgtcgaaggc cagaatcagc atcgccgcga atcgtgacaa gccggtgtcc
      481 ttcttcgcca tactcgagct cgacctcgac gcgcggcgcc ggcagaacgc cggctcccgc
      541 ttccggccac tcgacgaaga cgatcgcgtc gtcgaagtag ggctcgaggt ccccccactc
      601 cgctggggaa acgccgcgaa agcgaaacag gtcgaggtgg gacacgtcga cgcgaccctt
      661 gtaccggtga ccgatggtgt acgtggggct cgtgactgct ccctcgacgc cgagcgcccg
      721 gcaggcgccg cggacgaacg tcgtcttgcc ggatccgagc tcccccgaga ccgtcacgac
      781 gtcgcccggg cgcagatctg cggcgaggct cgcagcgacc tgctccgtct cttccggcga
      841 gcgagtctcg atcagaagag cccgagctgc acggcgggct ctgcgacgac cggcgcgacg
      901 taaacaggct cgggcggagc gacctcgcgg cccatcagct cgggcagcct cgcgaagtcg
      961 gacttgagca cctcgagcat gcgcggcgtg tacaacgcgg ccgccgggtg atagatcgga
     1021 tagagcaata cacgacgtcc gccgagcgtc acctcctgct cctggccatg gacgcgtgtg
     1081 attccggtcg gccgtcccga caggagcttg gtcgcgaagt tcccgagcgt cgccacaacg
     1141 cgtggctcga tcaattcgat ctggcgccag agatgcgact cgcacgcctg gatctcttcc
     1201 ggcagcggat cacggttgcc gggagggcgg catttgagaa cgttcgcgat gtacacctca
     1261 tcgcgtgcaa ggccgatgcc tgcgagcagt tgctcgagca gcttcccggc agcgccgacg
     1321 aagggcactc cctgcttgtc ctcgtgaaag cccggtgcct cgccaacgaa catcaggtcg
     1381 gaatccggat cgccggcgcc gaacacgact tgagtgcggc ccgctgcgag cgtgcacttg
     1441 gtgcagtcag cgacgagcct gccgaaggcg tggagctcgt ctgcccgggt cagctcagtg
     1501 accgcagcca cagctcccgc cgcaacagcc ggtgccactg ctgaacgcgc cggtctgccc
     1561 ggcggtgccg accatcgaga acgtcgagag ctgccgggcg acattggtcg cgccacactc
     1621 cgggcaggca accacctgat ctccccggac gagctcctcg aagtgctcct cgcacttcat
     1681 gcagacgtac tcgtacatcg gcatcgctcg ggcagtttag gtgcccgtcc ggacgggcgg
     1741 caactggaac tagtaggagc ccttgaccac gcggccgccg agcctaccgg agaccgaatc
     1801 cttcgagaac gtcagcgtcc cccggacggc cgaggagccc gacacacgga gcgtcccctt
     1861 gtaggcaagg ggcaagcctc cggggacgaa agagaccttc cctgtcacct ggatcccggc
     1921 aacggcgctg taccgggtca gcgtgaagct gagcgcctgc tgcgacagga cgagcttgcc
     1981 tccgtacaga ccgcggggag caaacgacgc cgaggagagc acgatctgca gccaggtcgc
     2041 ctcggcctcg cgaaccgtct tcgctgcagc cgcgagggtg gccttcacgg tcgcctttgc
     2101 cggtcggcgc gggaacgcgc cgaggatctt cccgacgggt gggacccgcg gacaggaggc
     2161 ttttcggaag ttctgtagcg ttccgaggat ccactctctg atcgcgtcgg acgcgcaacc
     2221 ggagaagtcg gcggaggaca cgctgtgtcc gacgccgggg acggtgatca gctggccctt
     2281 gggaaacgcg gagatcactg cgtccgcatt cgccgtcggc gtccgtaggt cgaacccgcc
     2341 gttgagggcg agcacgggca cgtttgggag cggcccgggc ccgagcggcg tgtttcctgt
     2401 gggcgtcggc cactgctcgc agaagtaggc cgtcccgatg cgggccgccc agtttccgaa
     2461 tggcccgacg ctaccgggtg gaaaggcccc gatcgttgca tcgaggatcg ctctgcgcgc
     2521 ggccggcgga gtatccgggg cgaacgggaa gcgaccgtct gcgcaattcg tcgcggcata
     2581 gagaccgaag ctcagatctt ccgccgacag cttgctcgtc cgcaggtcca ggtcgtacaa
     2641 gcggagcaag gggcgcgcgt tcccgagcag ggcggcgtga accgcagccg gcgcctcggc
     2701 ggcaaggccg ggcgagagat ccgcgtcgat gatcatcgag atcagctctt caccgttcat
     2761 ttgcaaggcc cgcagcttcc cgttgggtgc gatgaccttg cccttgacgg gccgggcttc
     2821 gagtcgattc gcaagcttga cgacgtcacc cgcgaaatcc cgagtggccc cgttgcagat
     2881 gccgcccgca cagaaagcgg cgagcgtgcc cggcatcgct cgcaggacat ttcgctcgaa
     2941 ggggtcggga aacgtggggg cgacaacgga gtcgagggcg attcgctcga cgacggcggg
     3001 atgagcgagc gcgtaagcga gggcaagctt ggtcccgtac gaaaccccga agagcccgat
     3061 tcgcccgagg cccaacgcaa cccgcacgga ctccatgtct tctgcgtggt cgcgggtcgc
     3121 atagaactgt cgccccggtc cgatgatcgc agcgcagtcg cgggcgagtg cggcgtcctg
     3181 ttcgatcgtc gtcatcgtcg agttctgcag gccgggacag ttgatgaggc cggattgtcc
     3241 tgtgcctcga ttgtcgaatg cgacgagcgt gtagccgggg aacatgttct gcaagtcctg
     3301 ggcagagccg gtagagccaa ggccgtaggc gccggcagat ccctgccccg ggccgcctgc
     3361 gatcaagaac atcgtcccgc gaggtaaccc actggccggc aggacctcga cgtggagcgg
     3421 gatcgtgccg ggcacggctc cgctccgatc caacgggaca tcgacggtcc cgcactggac
     3481 gccgttcgtg gtctccggac aaggcgagag ggcgatcgcc gcacgcgcct ggccgggtgc
     3541 ggccagcaca acgacgacgg ctagccccag gaggaaacga cgacccacca ggcacggcag
     3601 ggtaccaccg gcggtgtacg cttcccctgc ccggtatccg ctacggaact ggagcgtcga
     3661 gtgttcgaga gcgtcaaagt catcgggtcg ggccgcgtcg gctcggcgat ttcggctcgc
     3721 ctgcgcgcga gggggctaac cgtgggcgac gaccgggagc tcgtcctcct gtgcgtgccg
     3781 gacggctcga tcgccgaagt ggcagccggc ctcgaggaag gcccctgggt cgcccacgtc
     3841 tccggagcca ggcctctgtc ggcgctcgat ccgcatcgcc gccgtttctc gatccacccg
     3901 ctgcagacgt tcactcacgc gcgaggggcc gagcagctcg acggagcctg ggccgctgtg
     3961 acggccgaat ccgacgaggc cagggttgcc ggtttctggc tcgcgga
//
LOCUS       DASVEU010000225        18371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_934145,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000225 DASVEU010000000
VERSION     DASVEU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 18371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18371
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934145"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF225_09690"
     CDS             <1..230
                     /locus_tag="VF225_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7145564.1"
                     /translation="CAQDPERIVVDLGEVEFIDSTALGVLIEIRAKLDNRDGLLLAAP
                     GLETRRALEVSGLDKLFVVRDSVQDALGAKV"
     gene            complement(227..1657)
                     /locus_tag="VF225_09695"
     CDS             complement(227..1657)
                     /locus_tag="VF225_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145565.1"
                     /translation="MTSNPHRWRILAVVATAFFMTILDVSIVNVALPSISRSLHFSRE
                     NLQWVITAYSIAFGGFLLLAGRAADLLGRRRVFMVGVALFTVASLVCGLAQSEAMLIV
                     ARAVQGLGGAIITPAALSIVMTSFEEGAERNKALGIWGALGGSGAAVGVLAGGVLTKY
                     LGWEWIFFVNVPVGALVLALTPRIVPESRREGAERRYDALGAVLVSGGLALLVYTISR
                     APDVGWATARTILLLVASGALLVGFLVNERRAPDPLMPFHIFRVRTVAGANVVGLLLG
                     SVIFANFFLLTLYVQNVLGYSALKTGVTFVATAGTAVVAAGVAQALTTKFGPRPIIAI
                     GLALLTGAMLWYSQIPVHGSYVSHLLPGYLMVGVGIAFAFVPVSIAALAGVAEREAGL
                     ASGLINTSQQIGGAIGTAVASTIFTSHFKTLRLEGKSLPVALTGGYSWAFYGLAVFGV
                     LAFIAAVTLIGREDMSQAPSTAPAGS"
     gene            complement(1869..3254)
                     /locus_tag="VF225_09700"
     CDS             complement(1869..3254)
                     /locus_tag="VF225_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7145566.1"
                     /translation="MASSDDPSRREGRYDRRELIRRAGAAGISIGVAGSGFGQAFYGP
                     LRFKGRWLKGDLTIIQWAHFVPAYDKWFTDVWSKNWGQKNDVQVKVDLILNTLLPARA
                     AAEAASGSGHDLFFNLSPQAQFEDQVINHAEIVQEVQRKVGKIGGLGKRATYNPKTKK
                     WFAFADNYVPDPVVWRHDLWNGVGIAPSTWENVRKAAPKLKAAGHPIGIGQSAELDSN
                     MALMAFMMCFGAYVQDEKHRVTVKSKHTIEAVKFMADIFKSGETDEIFGWTPASNNNF
                     LYSGKGSMILNAISATRTPEDLGLPFADQLYIYPIPKGPVRRMGLEHVMGSYSIWKFA
                     QNIPAAKKFMADLEINYKDAFVASKFYNFPTFPGALPFKRIQKLAAADTHKPRGKYQI
                     LTTIAQKYTVNPGYPGYTNAAFSEVFGKFLIPKMFAQVSQGKMSAADAVSAADHDFRD
                     IYTKWRKLGKI"
     gene            complement(3274..4149)
                     /locus_tag="VF225_09705"
     CDS             complement(3274..4149)
                     /locus_tag="VF225_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7145567.1"
                     /translation="MDAVLRGFREKGLSYLIVGPFAIVLAFPFYVMVITAFKQQRDFY
                     DLSHSPYWFHKGPTLSNARFLFDETQYTTWLRNTAIIGVVVVLITLVLAVPAGYALAR
                     LSGKLGQSVGVGIFLTYLVPPTLLFLPLFSVITQLDRQTSHLLHFHLRGSIWSLVLVY
                     PSFTVPFATWLLMGFFKTIPRELEDAALIDGASRLGTLVRIVFPISVPGILTVVIFSF
                     SLVVNEFVYAFTFISASSHRTLSTGVYTDLIRGDLPQWGAIMVASLIPSIPLALLYNT
                     FLDRFIAGFTGGAFR"
     gene            complement(4153..5088)
                     /locus_tag="VF225_09710"
     CDS             complement(4153..5088)
                     /locus_tag="VF225_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7145568.1"
                     /translation="MEALAASRPRGRRWTNVLERPSVLASVLISPAVIFILALVGGPL
                     ALAVYLSFTNAIGGSLHGRWIGLGNFRHEWTNPAFQDAVWHTFLFTVISQAIVLVVAG
                     LLAHALLKKFPGKWLLRFLILLPWAAPLPLSLMGWTWIFDTTYSIINWTLQHLHLVST
                     VSPPQWLAQPRLAMGSVIAVHAWRIIPFATVIFLAGLASIPKEVDDAAAIDGATGLRK
                     FWSVSLPLQLPIATVALLFGIVFTATDMAVVYILTNGGPPPSNSTEMVTTWAYKTGIE
                     SGSLGAGAAISLFLLPVLAVVAIGMLIFARRAEVT"
     gene            5128..6312
                     /gene="ugpC"
                     /locus_tag="VF225_09715"
     CDS             5128..6312
                     /gene="ugpC"
                     /locus_tag="VF225_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7145569.1"
                     /translation="MAEITLEKLTKVYGDGTRAVSELDLEIGDGEFVVLVGPSGCGKT
                     SALRMVAGLEPITGGRVVIGGEVVNKLPPKDRDIAMIFQNYALYPHMSAFDNMAFGLK
                     LRGMNKERRGERVNGAAQTLGLNEVLPKKPRTLSGGQRQRVAMGRAIVREPRAFLMDE
                     PLSNLDAKLRVEMRAEIARLQRELNVTTIYVTHDQVEAMTLGDRVAIMRDGLLQQVAK
                     PQDLYDRPRNLFVAEFIGSPAMNLIGVDLSYSDGRVVAKFGDSTLEVGEQVLVRRPGL
                     AAFEGKRIILGVRPEDLEDEAFATDSSPGRRLSAQVDIREDMGSEVFIHFGVAGKAVR
                     GADVKAAVGEDAIEVAEAKGNVWVARVDRDTDAQEGGSMELVVDTSRLHFFDPESGEA
                     IY"
     gene            complement(6306..6749)
                     /locus_tag="VF225_09720"
     CDS             complement(6306..6749)
                     /locus_tag="VF225_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7145570.1"
                     /translation="MEKIVVGIDGSESSKNALRWAVEDARVRGAEVIALHAYEEPMPG
                     ADATLAVPIDLPGLVTEFHEGALRFVTEVVDEVVGNAVTVNVAPVAVEDEAARALLDA
                     ARDADLLVVGSHGHGLSGLLLGSVSLECARHAACPVLIHRGSAPQ"
     gene            complement(6749..7273)
                     /locus_tag="VF225_09725"
     CDS             complement(6749..7273)
                     /locus_tag="VF225_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145571.1"
                     /translation="MKQTGLQLLGLAAISALLVATGCGSTKTVTRTVTVSPTAKNGVG
                     PPREVVEYGYIKSLKRKGATFELRFDPAWLLTGKTANQAALEDTGSSDVPNDNYIVNE
                     STRAYTFFVSPSAHVTVLKEGVNGSAITVGQLAQLADGRNPFPKPLFEPISTGFWISI
                     RNDTVGSLEQQYHP"
     gene            7441..7773
                     /locus_tag="VF225_09730"
     CDS             7441..7773
                     /locus_tag="VF225_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145572.1"
                     /translation="MKGSSPLLLAAAETRLACAPFTCCAVTFCSGACWSSGVLTGGAD
                     TEEGADPPDGVDGVLPFPGVPGEPGFVVVPGVVVVWFVVVVWFVVVVWFVVVVSFVVV
                     VSVVVVSV"
     gene            8279..8743
                     /locus_tag="VF225_09735"
     CDS             8279..8743
                     /locus_tag="VF225_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145573.1"
                     /translation="MKLFVVVILTAALGVVAPSAASAQTPAGYRAQLNRLCRGYTPKL
                     RADQKAMQKATEAKDWKAFGYALGHYLALALTQDRRIETTPVPHAMRTQMAPILRLLR
                     TADSHTRLALRKAALGDSRGMLAELDKIGKLAQPLNGRLDRAGLRDCGSNQS"
     gene            complement(8819..8891)
                     /locus_tag="VF225_09740"
     tRNA            complement(8819..8891)
                     /locus_tag="VF225_09740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8856..8858),aa:Thr,seq:cgt)
     gene            complement(8936..10192)
                     /locus_tag="VF225_09745"
     CDS             complement(8936..10192)
                     /locus_tag="VF225_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145574.1"
                     /translation="MAAEPSIGRATRVERPFAPGADAAGDLYERYSAQILSYCQHQLG
                     SREEAEDAVQTTFMNAFRGLSRGIVPELESAWLFKIAHNVCLSRRRSSWRRGKIEAPN
                     NFEVLQEVIPGREQISDELIGLQDVLEQMPEHQRRAILLREWQGLTYREISAELGLTQ
                     AAVETLIFRARRALAQGLEQDADTWKKRLRRGADVGGAIAFVKALLGGGGAAAVKVAA
                     TALAVAATTVAAATEVPRHAHHAPATKPTPAVIQHLTRESTATIASPTATAPESLAHV
                     TSTRSLPKPVALPVSHPEQTAVVSATTEPSSVPETPPAPAPSRPDPTPPPAASAASVP
                     SAPAGNGNGNGKGKKDERTTQATTQTTETPAVSPAVDPPAQQIDQGQGNDNKDKSVKP
                     GNGNADASNSNGNGNNGNSNGNGKNK"
     gene            complement(10295..11380)
                     /locus_tag="VF225_09750"
     CDS             complement(10295..11380)
                     /locus_tag="VF225_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX7145575.1"
                     /translation="MDVFENIPEQAEAKRLLRAALADEGPAHAYLFHGPPGVGKRAAA
                     IAFAGELIGDRGRVERGSHPDLYLVEPVGDQIRIDDIRELRRDLHMRPFEADRRVYLV
                     YAAETMNEDAADALLKDLEEPPPYAVMVLVADDLGPLPETIRSRCQLVPFTRLSERAI
                     REEIDARAPGLEPDQAASLARLASGRLDRAARLLDPDSARRREALLEVARSVYTDAEF
                     EPGEAAQRILDGAKECGAEAKLKAEEGLELLDLPTREAEQRVRRAARGAEREELLASL
                     EELASWYRDLVVVAVGAERAVVHADRRELLAQDGTTERLAGAERACELVRETWRAFEE
                     FNLSPPLALEALFVKLRRELGTTPALV"
     gene            complement(11355..11951)
                     /gene="tmk"
                     /locus_tag="VF225_09755"
     CDS             complement(11355..11951)
                     /gene="tmk"
                     /locus_tag="VF225_09755"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7145576.1"
                     /translation="MFVTFEGLDGSGKTTQAELLAEALRADGRDVVATREPGGTPLGE
                     EVRRLLLEGMEMSPWAEAALFAAARAELVERVIAPALDAGHDVVSDRYVDSSLAYQGH
                     ARELGIDEVYELNRVATRGLLPDVTFVLEVDLDTSVRRVGQSPDRIEREGRDFRQRVL
                     DGYRIVAGKYGERVVLLDGTRPPDELHEEIRGRLREHS"
     gene            11974..12879
                     /locus_tag="VF225_09760"
     CDS             11974..12879
                     /locus_tag="VF225_09760"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7145577.1"
                     /translation="MRAVVTGGAGFIGSHVVDALLARWDEVHVLDDFSRGKRENVPEG
                     VHVHEADIRTDSGRVFDEVKPEVCFHLAAQADVRVSVERPDFDADVNVVGTLHVLEAA
                     REHGTKIVFSSTGGAIYGECEGPAREDHPRAPLAPYGVSKLAGEEYIAAYNRLYDAAH
                     VSLRYGNVYGPRQDPKGEAGVVAIFMNRLREGEAPKIFGDGTQTRDYVYVGDVVSATL
                     AAAEHAGGVLNVGTGVETSVLELYERIQHVAGVEREPEFADARPGELQRSVLDASLAK
                     RELDWEPQRTLDEGLAETWAWVSST"
     gene            complement(12984..13355)
                     /locus_tag="VF225_09765"
     CDS             complement(12984..13355)
                     /locus_tag="VF225_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145578.1"
                     /translation="MARTIAIVEDDADIRGLLDFKLRAAGYETAFARDGVSAIGVIRK
                     AQPDLILLDIGLPGGDGFVILDRLREFDALATIPVVVISAVVRSDTRERIELSGVAAF
                     VEKPFDVEELLRTVESILAAR"
     gene            13418..13921
                     /locus_tag="VF225_09770"
     CDS             13418..13921
                     /locus_tag="VF225_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX7145579.1"
                     /translation="MQHAQDLDLDVIRPWRRATIAVSAVAAIELIALAAIAFVALGNP
                     LATHLRETTAAAAGPPRVRTAQPAPAKKAALARADTSVMVLNGGGQAGAAHTAANQVS
                     AHGYQLGQIGNAAGDTPRTLIMYRPGYAAEGARLGRDLHVRIVRPLDGMRTAQLLGAH
                     LVLITGR"
     gene            complement(13908..15785)
                     /locus_tag="VF225_09775"
     CDS             complement(13908..15785)
                     /locus_tag="VF225_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7145580.1"
                     /translation="MRWPVAWGAAAGGLSFAALFFGEGSSQSRVFWIGAPAVLVAAVG
                     WAWRPPRLPLRGSIFFLSLAAFVVWQGVSIAWSIEPSRSWDYTNRGLVYFAFAAVGAL
                     VAGTPLRRFAWAGCLLLAALFFWALAAKVIPGLYPDYERLARLRYPIEYWNELALLAA
                     ASVPLGLWAAGPRHDRRARMGGVLLLYAALVVAVLTYSRVGIVLTVVAAIVWLWLDRD
                     RLAALGPLALAWIVGAVVAGVALLLPGVSADGQPHDVRVQDGLVFGAVFVVGAVVVGF
                     ASRFAVARTVDRRVARAAGVALVVLVLVALAAAVVRAGGPADFVSARWHEFSNKTSAQ
                     VENTPGRIVSASSSNRWRWWKEAWNAFTDKPLQGTGAGTFELVDRVARTSPLATIEPH
                     SVPLQFLSETGIVGLLLYGAVVASAAFGILRRERTRAWLALALGAAFCFVHSWVDIDW
                     DFIAVQGPLFLTVGALVSGPAAPMPARRRWLVSVAVGVSALAALYSVTSPWLATNRVA
                     AAYDAIERNRPDQALTDARSAHAFNPLSVDALFVQALVEPNAKALKLYRQARDLEPTN
                     PETWYQLGAFELLYLNRPRDAYRDLNHSYTLDNTLFSPASAAGHQLDEARCKVDPATC
                     P"
     gene            15973..16515
                     /locus_tag="VF225_09780"
     CDS             15973..16515
                     /locus_tag="VF225_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145581.1"
                     /translation="MPRFLKPLILVAAAVAIGAAALGTAQPAQARSMSTCSSALIHDW
                     YVDGRVDKTYPVHCYREALNEIPEDQAIYGTLRQDLTRALQATIRQHGGHVTGDTLVV
                     PLGGGGGNGPNGTGSSGGIFHWLAQKLGPSTADSIPVPLLILGGLAFALMAAAGVSLI
                     ARRMQARRAAANPPPAGPSF"
     gene            complement(16512..17639)
                     /locus_tag="VF225_09785"
     CDS             complement(16512..17639)
                     /locus_tag="VF225_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145582.1"
                     /translation="MARKEGNMRARCLLVIAAVVALTLGTAQTALADNDATGSVGAVQ
                     TGPVGVTPTADASQAGATVAASVPVAVGGSGNNTATNSVGAVQAGGGNTSNDSTGSVQ
                     VSSVSASPSASAGASGSSASAGVPVTVGGDGSNNASSSTGAAQVGGGNSSGDSTGVLQ
                     SGSPQASPAVGASLVGEAVSASAPVGVAGSGNSAADAVGAAQVGGGNTVTRSFLATQS
                     GAVTAGTTAGAGGTTTSAPVTVGGGGGTNSATDSIGTAQVGGGNTATGSTGTVQSGPV
                     GATPSVASGPTATAASTPAASASTPASATGVPSAPGTRTSEGAPRPNRNPFGATKTPT
                     GGPQTLGQLPFTGLGLLLFVLLGLTLVATGLTTRARTRLSV"
     gene            complement(17708..>18371)
                     /locus_tag="VF225_09790"
     CDS             complement(17708..>18371)
                     /locus_tag="VF225_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7145583.1"
                     /translation="ASAKGVVVVAAAGNAGTTTQFYPAADPHAIGVAATTVADGRYSW
                     SNFGSWVRLAAPGCNIAPTLGGGYGTFCGTSSATPVVAGLVALELSAQPAATPREAED
                     ALVRAAVPLPAFVQYGRIDAGRTLALLRPAAATSAVFRGTLGSRVRARTYNVDVGAGA
                     FSATLRFTGGKRLTLVTPTGRATGNSPIQLNGTSAAGTFTLRVGGTGAKTSFVLTVTY
                     VR"
BASE COUNT         3159 a         6196 c         6173 g         2843 t
ORIGIN      
        1 cctgtgccca ggatccggag cggatcgtcg tcgacctcgg cgaggtcgag ttcatcgact
       61 cgacggcgct cggcgtcctg atcgagatcc gcgcaaaact ggacaaccga gacggcctcc
      121 tgctcgcagc gcccggtctc gagacgcggc gcgctctgga ggtctccggg ctcgacaagc
      181 tcttcgtcgt gcgcgacagc gtgcaggacg cgctcggcgc aaaggtttag ctacctgcgg
      241 gcgccgtcga cggtgcctgc gacatgtcct cgcggccgat cagcgtgacc gccgcgatga
      301 acgccagaac accgaacacg gccaggccgt agaaggccca gctgtagccg ccggtcaatg
      361 ccaccggcaa cgacttgccc tcaaggcgaa gcgttttgaa gtgactcgtg aaaatcgtcg
      421 acgcaactgc agttccgatc gccccgccga tctgctgtga cgtgttgatg agcccggagg
      481 ccagtcccgc ctcgcgttcg gcgacgccgg cgagcgctgc gatcgagacc gggacgaacg
      541 cgaacgcgat ccccacgccg accatcaggt agccgggcag caggtgggac acgtaactcc
      601 cgtgcaccgg gatctgcgag taccagagca ttgcgcccgt gagtaatgcg aggccgattg
      661 cgatgatcgg cctcgggccg aacttcgtcg tcaatgcttg agcaactccc gcggcgacca
      721 ctgccgtacc cgcggtcgcg acgaatgtga cgccggtctt cagagccgag tagccgagaa
      781 cgttctgcac atagagggtg aggaggaaga agttggcgaa gatcaccgag ccgaggagca
      841 ggccgacgac gtttgctccc gcgaccgttc gcactcggaa gatgtggaag ggcatcagcg
      901 ggtccggggc tcgacgctcg ttgacgagaa agccgacgag caatgcccca gaggccacga
      961 gcaggagaat cgtccgcgcg gtcgcccagc ccacatcggg cgctctcgag atggtgtaga
     1021 cgagcaacgc caggccgccg ctgacgagca ccgcgccgag cgcgtcgtaa cgccgctccg
     1081 cgccctcacg cctgctttcg ggaacgatgc ggggcgtcaa ggcgagcact agcgcgccga
     1141 ccggcacgtt gacgaagaag atccactccc agcccaggta cttcgtcagc acgccgccgg
     1201 cgagcacgcc gacagccgcg ccgctgccgc cgagtgcacc ccagattccc agcgccttgt
     1261 ttcgctccgc accctcctcg aagctcgtca tcacgatcga gagcgcggcc ggcgtgatga
     1321 tcgcgccgcc gagtccctgt acggcgcgcg ccacgatcag catggcttcg gattgcgcga
     1381 gcccgcagac gagcgaggcg accgtgaaga gcgcgactcc gaccatgaac acccgtcgcc
     1441 ggccgagcag gtccgcggca cgcccggcga ggaggaggaa gccgccgaag gcgatcgagt
     1501 acgcggtgat gacccactgc aggttttccc gcgagaaatg gagcgagcgg ctgatcgagg
     1561 ggagcgcgac gttcacgatc gagacgtcga ggatcgtcat gaagaaggcc gtcgcgacga
     1621 cggcgaggat cctccagcgg tgcgggttcg aggtcacgtc tgcgtaggtt aatagtcgag
     1681 cccgacgcta cccaaggagt cacgtcctga actgcaaggg tctgacccca gcccttcggg
     1741 tcacgtccga gtgagtcacg ccccgaactg caagggtctg accccagctg ttggggtcac
     1801 gtcacgttcc ggcaggtgag gttgcggacg gccgcggcta cgaccgcccg cagtctctca
     1861 gcgggagctc agatcttccc cagcttccgc cacttcgtgt agatgtccct gaagtcgtgg
     1921 tccgcagcac tgaccgcgtc ggcagcgctc atcttgccct gcgagacctg ggcgaacatc
     1981 ttcgggatca ggaacttgcc gaacacctcg gagaacgcgg cgttcgtgta gcccgggtag
     2041 ccggggttga ccgtgtactt ctgcgcgatg gtcgtgagga tctggtactt gccacgcggc
     2101 ttgtgcgtgt cggccgccgc aagcttttgg atcctcttga agggaagcgc ccccgggaac
     2161 gtcgggaagt tgtagaactt cgacgcgacg aacgcgtcct tgtagttgat ctcgaggtcg
     2221 gccatgaact tcttggccgc cggaatgttc tgagcgaact tccagatcga gtaggacccc
     2281 atcacgtgtt cgagccccat ccgccggacc gggcccttgg ggatcgggta gatgtagagc
     2341 tgatccgcga acggcagtcc gaggtcctcg ggcgtcctcg tcgccgagat cgcgttcagg
     2401 atcatcgagc ccttgccgga gtagaggaag ttgttgttcg aggccggcgt ccagccgaag
     2461 atctcgtcgg tctcaccact cttgaagatg tcggccatga acttgacggc ctcgatcgtg
     2521 tgcttcgact tgaccgtgac gcggtgcttc tcatcctgga cgtacgcgcc aaagcacatc
     2581 atgaaggcca tcagcgccat gttcgagtcg agctccgccg actgaccgat cccgatcgga
     2641 tggccggcgg ccttcagctt cggcgccgcc ttgcgcacgt tctcccaggt cgatggtgcg
     2701 atgccgacgc cgttccagag gtcatgccgc cagaccacgg ggtccgggac gtagttgtcg
     2761 gcgaaggcaa accacttctt cgtcttcggg ttgtacgtcg cccgtttgcc gaggcctccg
     2821 atcttcccga ccttgcgctg aacttcctgg acgatctcgg cgtggttgat gacctggtcc
     2881 tcgaactgcg cttggggcga gaggttgaag aagaggtcat ggccggaacc ggacgccgcc
     2941 tccgctgcag cacgagccgg caacagcgtg ttgaggatga ggtcgacctt gacctggacg
     3001 tcgttcttct gcccccaatt cttcgaccac acgtctgtga accacttgtc gtaggccgga
     3061 acgaagtgcg cccactggat gatcgtcagg tcgcccttca gccagcggcc cttgaagcga
     3121 agcggcccgt agaacgcctg cccgaagccc gagcccgcca cgccgatcga gatgccggcc
     3181 gcaccggccc tccggatcag ctcgcgacgg tcatacctcc cttcgcgtcg actgggatcg
     3241 tctgagcttg ccactacccc tcctttctag gccctaccgg aatgctcccc cggtgaagcc
     3301 cgcgatgaac cggtcgagga acgtgttgta gaggagcgcg agcggaatgc tcggtatgag
     3361 cgaggcgacc atgatcgctc cccattgcgg cagatcgccg cggatcagat ccgtgtagac
     3421 acccgtggac agcgtgcggt gactcgatgc agagatgaat gtgaacgcgt acacgaactc
     3481 gttcacgacg agcgagaagg aaaagatcac gacagtcaag atgcccggaa cagagatagg
     3541 aaaaacgatg cggaccaggg tcccgagccg tgaagcgccg tcgatcaggg ccgcgtcctc
     3601 gagctcccgc ggaatcgtct tgaagaatcc catcaacagc cacgtcgcga acgggacggt
     3661 gaacgaggga taaaccagga caagcgacca gatcgagcca cgcaggtgga aatgcaagag
     3721 gtggctggtc tgtctgtcga gttgcgtgat caccgagaac agcggcagaa agaggagcgt
     3781 gggcggcacg aggtacgtca ggaagatgcc gacgccgacg ctctggccga gcttgcccga
     3841 aagccgcgcg agcgcgtaac ccgccgggac cgcgagcacg agcgtgatca gcacgacgac
     3901 aacgccgatg atcgccgtat tgcgcagcca cgtcgtgtac tgcgtttcgt cgaaaaggaa
     3961 acgggcgttg gagagcgtcg gtcccttgtg gaaccagtac ggcgaatggc tgaggtcgta
     4021 gaagtcgcgc tgctgcttga aagcggtgat caccatcacg tagaacggaa aggccagcac
     4081 gatcgcgaac gggccgacga tcagatacga cagccctttc tcgcggaacc ctcggagcac
     4141 ggcatccatt tgctaggtca cctctgcgcg gcgcgcgaag atcagcatcc cgatcgcgac
     4201 gacggcgagg accggtaaga ggaacaggga gatcgcagcg cccgcgccga gcgaaccgga
     4261 ctcgatcccg gtcttgtagg cccaggtcgt gaccatctcg gtggagttgg acggcggcgg
     4321 gcctccgttg gtgaggatgt agacgaccgc catgtccgtc gccgtgaaga cgatcccgaa
     4381 cagcagggcg acggtggcga tcggcaattg caacggcagc gagaccgacc agaacttgcg
     4441 cagtcccgtc gcgccgtcga tcgccgcggc gtcgtcgacc tccttgggaa tcgaggcgag
     4501 accggcgagg aagatcacgg tcgcgaaggg gatgatccgc cacgcgtgga ccgcgatcac
     4561 cgagcccatc gccagcctcg gctgcgcgag ccactgtggt ggagagactg tgctcaccag
     4621 gtgcaggtgc tgcagcgtcc agttgatgat cgagtaggtc gtgtcgaaga tccaggtcca
     4681 gcccatgagg ctgagcggca gcggcgcagc ccacggcagg aggatcagga aacggaggag
     4741 ccacttgccg gggaacttct tcagcagggc atgagccagc agccctgcga cgacgagcac
     4801 gatggcctgc gagatgaccg tgaacaggaa cgtgtgccag acggcgtcct ggaaagccgg
     4861 gtttgtccac tcgtggcgaa agttgccgag gccgatccac ctgccgtgca gcgagccgcc
     4921 gatggcgttc gtgaagctca ggtagacggc gagcgcgagc ggaccgccga ccagcgcgag
     4981 gatgaagatg accgccggtg agatgagcac ggacgcaagg acgctcgggc gctccagaac
     5041 gttcgtccag cgcctccccc gcggccgtga agcggcaagt gcctccacca ggcggcggat
     5101 cctaatagag tttgaaacga tccgcgcatg gccgagatca cgctcgaaaa gctgacgaag
     5161 gtctacggcg acggaacccg ggcggtctcc gagttggacc tcgagatcgg cgatggggag
     5221 ttcgtcgtcc tcgtcggacc gagcggctgc ggcaagacga gcgccctacg gatggtcgcc
     5281 gggctcgagc cgatcaccgg gggccgcgtc gtgatcggcg gcgaggtcgt aaacaagctg
     5341 ccgccgaagg accgcgatat cgcgatgatc ttccagaact acgcgctcta cccgcacatg
     5401 agcgcgttcg acaacatggc cttcggtctc aagctccgcg gcatgaataa ggagcgccgc
     5461 ggcgagcggg tcaacggcgc cgcgcagaca ctcggcctca acgaggtgct cccgaagaag
     5521 ccccggacgc tgtccggcgg ccaacggcag cgcgttgcga tggggcgcgc gatcgtgcgg
     5581 gagccccgcg cgttcctgat ggacgagccg ctctccaacc tcgacgcgaa gctgcgcgtc
     5641 gagatgcgtg ccgagatcgc gaggctgcag cgcgagctga acgtgacaac gatctacgtg
     5701 acacacgacc aggtcgaggc gatgaccctc ggcgaccgcg ttgcgatcat gcgcgacggc
     5761 ctgctgcagc aggtggcgaa gccgcaggat ctctacgacc ggccgcgcaa cctcttcgtc
     5821 gccgagttca tcggctcgcc ggcgatgaac ctgatcggcg tcgacctttc atactccgac
     5881 ggccgcgtcg tcgcgaagtt cggcgacagt acgctggagg taggcgagca ggttctcgtc
     5941 aggcgcccag gactggcggc gttcgagggg aagcgaatca tcctcggagt ccgcccggag
     6001 gacctcgagg acgaagcgtt cgcgaccgac tcatcccccg gtcgccgact ctcggcgcaa
     6061 gtggacatcc gggaggacat gggctccgag gtgttcatcc acttcggtgt cgccggaaag
     6121 gcggtgcgcg gcgccgacgt gaaggcggcc gtcggcgaag acgcaatcga ggtggcggag
     6181 gccaagggca acgtgtgggt ggcgcgggtc gaccgcgaca ccgacgcgca agaggggggc
     6241 agcatggagc tcgtcgtgga cacgagccgc ctccacttct tcgacccgga gagcggcgaa
     6301 gcgatctact gaggcgctga gcctcggtga atcagcacgg gacaagcggc gtgccgtgcg
     6361 cattcgaggc tgaccgagcc gaggagcagg ccgctcagcc cgtgaccgtg cgagccgacg
     6421 acgagcaggt cggcgtcgcg agccgcgtcg agcaaggccc tcgcagcttc gtcttcgacg
     6481 gcgaccggcg cgacgttcac ggtcacggcg ttgcccacga cctcgtcgac gacctccgtg
     6541 acgaaccgca gagcgccttc gtgaaattcg gtgacgagcc cgggcaagtc gatcggcaca
     6601 gcgagggtcg catcggcccc cggcatcggc tcttcatacg cgtggagcgc gatcacctct
     6661 gcgccgcgaa cgcgcgcgtc ctcgaccgcc cagcgaaggg cgttcttcga tgactccgag
     6721 ccatctatgc cgacgacgat cttctccatc acgggtggta ttgctgctcg agcgaaccga
     6781 ccgtatcgtt tctgatgctg atccagaagc cggtcgagat cggctcgaag agcggcttcg
     6841 gaaacggatt gcggccgtcg gcgagctggg cgagctgacc cacggtgatc gcgcttccgt
     6901 tcacaccctc tttcagcacg gtcacgtgcg cgctcggcga aacgaagaag gtgtacgcgc
     6961 gcgtgctctc gttgacgatg tagttgtcgt tcggaacgtc actcgagcct gtgtcctcga
     7021 gggccgcctg gttcgcggtc ttccccgtga gcagccaggc agggtcgaag cgcagctcga
     7081 aggtcgcgcc cttgcgcttg agcgacttga tgtacccgta ctcgacgacc tcgcgtgggg
     7141 gaccaacacc gttcttcgcc gtcggggaca cggtgacggt tcgggtgacg gtcttcgtgc
     7201 ttccgcatcc cgttgccact aacagagcgc tgatcgcggc aagaccgagc agttgaaggc
     7261 cggtctgctt cacagcgtca gtctaagccg acgccgggct tgaaacggaa catcgcggtg
     7321 gcccgaatgg gccaccgcga tgggacgtgc taagaggcga ctcttgcgac tctgcgaagc
     7381 ccgagcccaa ctgcgagcat cgctgagccg agcagggcaa gagcccaggc ggggaacccg
     7441 gtgaaaggca gctctccgct cttgttggca gccgctgaga cgcgactcgc gtgtgcgccc
     7501 ttcacctgct gcgccgtaac cttctgctcc ggcgcctgct ggagctccgg cgtgctgacc
     7561 ggtggagccg acaccgagga gggcgccgac ccgccggacg gggttgacgg tgtgctgccg
     7621 ttcccaggag tcccaggcga acctggattc gtagtcgtcc caggggtggt ggtcgtctgg
     7681 ttcgtggtcg tggtctggtt cgtggtcgtg gtctggttcg tagtcgtcgt ctcgttcgtg
     7741 gtcgtggttt ccgtcgtggt cgtatcggtt tgattgcagt ggtcttcgtt gcccttggcg
     7801 ttcccgtgat cctcgcagtc cttcccgttc tggtttggat cggggcgaca atcaacgcta
     7861 ttgctgccgt gcgagcagtc gtcgccgggg gatccgggaa cggtcgtgga agcttggttt
     7921 ccgttcccgt tgccgttgcc gttcccgttg ccggaatcgg cgagggcggc cgacacgccg
     7981 acaccggcga tcagcagacc tgccatcgcc acgaccttcg cggccttcag tgtccatcca
     8041 gtcatgcaag tcctctcgtt tccgtggatg atgggctagc caccggacgc ccgtaccgca
     8101 cacgagcgcg gaatgccttc ccccctgtgc cccactttgg gaaaaggccg gatgacaggc
     8161 gcgccacgaa ggtactgatc tgcctggacc tagaggttgt cgctcgctgc cgcgtgacta
     8221 aaggcggtag ggcgaccgga cgatgaaagc tccagaaagg gaggcctacg ctttgccgat
     8281 gaagctcttc gtcgtagtca tcctcaccgc cgccctggga gtcgtcgctc cgtcggcggc
     8341 ttcagcgcag actcctgctg ggtaccgcgc gcaactcaac cgcctctgtc gcggctacac
     8401 cccgaaactc agagctgatc agaaggcaat gcaaaaggcg accgaggcca aagactggaa
     8461 ggccttcggt tacgcccttg gtcactacct cgcgctcgcg ctaacccagg acaggcggat
     8521 tgagaccacg ccggtccctc acgccatgcg cacacagatg gcgccgattc tgcgtctact
     8581 caggacggct gacagccaca ctcgactcgc cctccgtaag gccgctctgg gagacagccg
     8641 tgggatgctt gccgagctcg acaagatcgg caagctcgcc cagcctctca atggtcgcct
     8701 tgaccgcgcc ggtctccgcg actgcggctc caaccagagc taggaagacg agggaccgag
     8761 ctgccgtaga gtcgcccacg ctcgccacgc gaggcccccg attcgccttc ggccaaagag
     8821 ccgacgccca gattcgaact ggggacccct tcattacgag tgaagtgctc taccagctga
     8881 gctacgtcgg cgaggcctct actttagccg cttcggaccc gggcaaggtt tcgaattact
     8941 tgttcttgcc gttgccgttg ctgttgccgt tgttgccgtt cccgttgctg ttgctcgcgt
     9001 ctgcgttgcc gttgccgggc ttgacggact tgtccttgtt gtcgttgccc tggccctggt
     9061 cgatttgctg cgcgggcgga tccacggccg gcgaaacagc cggcgtctcg gtcgtctggg
     9121 tcgtcgcctg agttgtgcgc tcgtccttct tgcccttccc gttgccattg ccgttaccgg
     9181 cgggggctga cggaacggat gcagcgcttg cagccggcgg gggcgtcggg tcgggccgac
     9241 tcggtgcagg tgcgggcggt gtctccggta ctgacgaggg ctccgtggtg gcggatacga
     9301 cagccgtctg ttctggatgc gataccggaa gagcgacagg cttcggcagg ctacgcgtcg
     9361 aggtcacatg cgcaagggat tcgggcgcgg tagcggtcgg tgaagcgatc gtcgcagtgc
     9421 tctcacgcgt gaggtgctga ataacagcag gcgtgggctt ggtcgccggt gcgtgatgag
     9481 cgtgcctcgg cacttcggtc gccgctgcca ccgttgttgc ggcaaccgcg agtgcggtcg
     9541 cagcaacttt cacggctgcc gcgccgccgc cgccgagcag tgctttcacg aatgcgattg
     9601 caccaccgac gtcggcgccg cggcgcagcc gtttcttcca cgtgtctgcg tcctgctcca
     9661 ggccctgagc gagcgcacga cgtgcgcgga agatcagtgt ctcgacggca gcttgagtaa
     9721 gcccaagctc ggccgagatc tcgcggtatg tgagcccctg ccactcccgc aaaaggatcg
     9781 cgcgcctctg gtgctccggc atctgctcga ggacgtcttg caagccgatc agctcgtcgc
     9841 tgatctgctc gcgtcccggg atgacttcct ggagcacctc gaagttgttc ggcgcctcga
     9901 tcttgccgcg gcgccaggag gagcgccggc gagagaggca cacgttgtgc gcgatcttga
     9961 acagccaggc ggattcgagc tcggggacga tcccgcgcga caagccacgg aaggcgttca
    10021 tgaacgtcgt ctggactgcg tcctccgcct cctcgcgcga gccgagctga tgctggcagt
    10081 agctgagaat ctgagcggag tagcgctcgt agagatcgcc tgcggcatcg gcgccgggag
    10141 cgaagggtcg ctcgacccgg gttgcccgac ctatggatgg ttctgcggcc accgatcctc
    10201 ctgctgctgc gggtacgaac gctccgcccg gaggcgctcg tactgcgagt gtcggcagcc
    10261 ctcaggaggt ggacgaccct agatcgaggg gtggctagac cagggccggg gtcgtcccca
    10321 gctcgcgtcg cagcttcacg aagagcgcct cgagggcgag cggcgggctg aggttgaact
    10381 cctcgaaggc gcgccaggtt tcgcggacga gctcgcaggc acgctcggcg cccgcgagcc
    10441 gctcggtcgt gccgtcctgg gcgaggagct cgcgccggtc ggcgtggacg acggcgcgct
    10501 ccgctccgac cgccacgaca acgaggtcgc ggtaccagga tgcgagctct tccagcgacg
    10561 cgagcagctc ttcccgttcg gcgccgcggg ctgcccggcg gactcgctgc tcggcttcgc
    10621 gggtagggag gtcgagcagc tcgagcccct cctctgcttt cagtttcgcc tcggcgccac
    10681 actccttcgc gccgtcgagg atgcgctgcg cggcctcgcc gggctcgaac tccgcgtccg
    10741 tgtagacgga gcgtgcaact tcgagcaagg cctcgcgccg acgcgcgctg tccggatcca
    10801 ggagccgtgc ggcgcggtcg agccgcccgg acgcgagccg cgcgagcgag gcggcctggt
    10861 ccggctcgag ccccggcgcg cgcgcgtcga tctcctcgcg gatcgcccgc tccgaaaggc
    10921 gcgtgaacgg aaccagctgg cagcgcgagc ggatcgtctc cgggagcggc ccgagatcgt
    10981 cggccaccag caccatgacg gcgtacggcg gcggctcctc cagatccttg agcagcgcgt
    11041 ccgccgcgtc ctcgttcatc gtctccgcgg cgtagacgag gtagacgcgc cggtcggcct
    11101 cgaagggacg catgtgcagg tcgcggcgca gctcgcggat gtcgtcgatg cggatctggt
    11161 ctccgaccgg ctcgacgaga tagaggtcgg gatgggagcc gcgctcgacg cggccgcggt
    11221 ctccgatcag ctcgccggcg aaagcgattg ctgcggcgcg cttgccgacg ccgggaggcc
    11281 catggaagag gtaggcgtgt gcggggccct cgtcggcgag cgcggcacgc agcagccgct
    11341 tcgcctcggc ctgttcagga atgttctcga agacgtccac gaatctcctc gtgcagttca
    11401 tcaggtggcc gcgtgccgtc gagcaggacg acgcgctcac cgtacttgcc ggcgactatg
    11461 cggtatccgt cgagcacgcg ctgccggaag tcccgtccct cccgctcgat ccggtcaggg
    11521 ctctgcccga cacgtcgcac ggaggtgtcg agatcgacct cgaggacgaa ggtgacatcc
    11581 gggaggaggc cgcgcgtcgc gacccgattc aactcgtaga cctcgtcgat gcccagctcg
    11641 cgcgcgtggc cctggtaggc gagcgacgag tcgacgtacc gatccgagac cacgtcgtgg
    11701 ccggcgtcga gcgccggcgc gatcacgcgc tcgacgagct ccgctcgggc cgccgcgaag
    11761 agcgcggcct ccgcccaagg gctcatctcc atcccctcca ggagcaggcg ccgcacctcc
    11821 tcaccgagcg gcgtgccgcc gggctcccgg gtagcgacga cgtcgcgtcc gtcagcgcgg
    11881 agcgcctcgg ccaggagctc cgcctgcgtg gtcttgccgg acccgtccag gccttcgaag
    11941 gtgacgaaca ccgccgtccg tacgatacgg cgcgtgcggg cggtcgtgac aggaggcgcg
    12001 ggcttcatcg gctcgcacgt ggtggatgcg ttgctcgccc gctgggacga ggtgcacgtg
    12061 ctcgacgact tctccagagg gaagcgcgag aacgtcccgg aaggcgtgca cgtccacgaa
    12121 gccgacatcc gcaccgactc ggggcgagtg ttcgacgagg tgaagcccga ggtctgcttt
    12181 cacctggcgg cgcaggccga cgttcgcgtg tcggtcgagc ggccggattt cgacgccgac
    12241 gtgaacgtcg tcgggacatt gcacgtgctc gaggcggcgc gtgagcacgg gacgaagatc
    12301 gtcttcagct cgacgggcgg cgcgatctac ggggaatgcg agggacccgc gcgcgaggac
    12361 catccgcgtg ccccgctcgc gccctacggg gtgtcgaagc tcgccggcga ggagtacatc
    12421 gccgcgtaca accggctcta cgacgccgcg cacgtgtcgc tgcgctacgg caacgtctac
    12481 ggaccgcggc aggatccgaa gggcgaagcc ggcgtcgtcg cgatcttcat gaaccggctg
    12541 cgtgagggcg aggcgccgaa gatcttcgga gacgggacgc agacacggga ctacgtgtac
    12601 gtcggcgacg tcgtctcagc gacgttggct gcggcggagc atgcgggtgg cgtactgaac
    12661 gtcggcacag gcgtcgagac gtcggtgctc gagctgtacg agcggatcca gcacgtcgcc
    12721 ggcgtggagc gcgagccgga gttcgcggac gcccgtccgg gtgagctcca gcgcagcgtc
    12781 ctcgacgcgt ccctcgcgaa gcgcgagctc gactgggagc cgcagcgcac cctggacgaa
    12841 ggcctcgccg aaacgtgggc ctgggtctcc tccacgtaag gagccacgtc tcgaacagca
    12901 agggtctgac cccagccctt cgggccaggt gcacaaagag ccacgttctc gaacacgtgc
    12961 gcgacgaagg cgtcgacttg cgtctaccgc gcagccagga tcgattcgac ggtccgaagg
    13021 agctcttcga cgtcgaacgg tttctcgacg aaggctgcga cgcctgacag ctcgatcctc
    13081 tcccgggtgt ccgaccggac gaccgcgctg atgaccacca ccgggatggt tgcgagcgcg
    13141 tcgaactcgc gcaggcggtc gaggatcacg aaaccatcgc ctcctggcag tccgatgtcg
    13201 agcagaatca gatccggttg ggccttccgg atcacgccga tcgcagagac cccgtcccgg
    13261 gcaaaggccg tctcgtagcc ggccgccctg agcttgaaat cgagcagccc gcgaatgtct
    13321 gcgtcgtcct cgacgatcgc gatcgtccgt gccacgaggc accatcctac gaccctgtga
    13381 aggagcacgc ccgcggggga cgaactcgct ccgggcgatg cagcacgcgc aagatctcga
    13441 cctcgacgtg atccggccct ggcgccgggc gacgatcgcc gtcagcgccg ttgccgcgat
    13501 cgagctgatc gcactcgccg cgatcgcgtt cgtggccctc ggcaatccgc tcgcgaccca
    13561 cctacgcgaa acgaccgccg ccgccgccgg acctcctcgc gttcgcaccg cccagcccgc
    13621 gcccgcgaag aaggcggcgc tcgcccgggc cgacacctcc gtgatggtcc tgaacggcgg
    13681 cggccaggcc ggcgccgcac acacggccgc caaccaggtg agcgcacacg gttaccagct
    13741 cggccagatc ggcaacgccg ccggcgacac tccccgcaca ctgatcatgt accgccccgg
    13801 ctacgcagcc gagggagcgc ggctcggacg tgacctgcac gtccgcatcg tccggccgct
    13861 cgacgggatg cgcaccgcac agctgctggg cgcgcacctc gtgctgatca cgggcaggta
    13921 gccgggtcga ccttgcagcg cgcctcgtcg agctggtgac cggccgcact cgcggggctg
    13981 aacagagtgt tgtccagcgt gtacgagtga ttcagatcgc ggtaagcgtc acggggacga
    14041 ttgaggtaca gcagttcgaa ggcgccgagc tggtaccacg tctccggatt cgtgggctcg
    14101 agatctcgcg cctggcgata gagcttcagc gccttcgcgt tcggctctac gagcgcctgc
    14161 acgaacagcg cgtcgacgga aaggggattg aacgcatgtg ccgacctcgc gtcggtgagc
    14221 gcctggtccg gacggtttcg ctcgatggcg tcgtacgcag ccgccacgcg gttcgtcgcg
    14281 agccacggtg acgtcaccga atagagcgcc gccagcgcgg agacgccgac cgcgacggag
    14341 acgagccacc ggcggcgggc aggcatcgga gccgcaggac cggacacgag cgctcccacg
    14401 gtaaggaaca gcggcccctg caccgcgatg aagtcccagt cgatgtccac ccacgagtgc
    14461 acgaagcaga acgcagctcc cagagcgagc gccagccagg cccgcgttcg ctcgcgccgg
    14521 aggattccga acgccgcaga cgcgaccacc gccccgtaca acaggagccc gacgatcccc
    14581 gtctcgctta ggaactgcag cggcaccgaa tgaggctcga tcgtcgcgag cggcgaggtg
    14641 cgcgcgacgc ggtcgacgag ctcgaacgtt cccgccccgg ttccctgcag aggcttgtcc
    14701 gtgaacgcgt tccacgcttc cttccaccag cgccagcggt tgctcgagct cgcgctgacg
    14761 atccggcccg gcgtgttctc cacctgcgcg ctggtcttgt tcgagaattc gtgccagcgc
    14821 gcgctgacga aatccgcggg cccgcccgcg cgcaccaccg ccgcggccaa cgccacgagc
    14881 accaagacga cgagcgcaac ccccgctgca cgcgcgacgc gccggtcaac ggtccgcgcc
    14941 accgcgaacc gcgacgcgaa tccgacgacg accgcgccaa cgacgaacac cgccccgaac
    15001 accagcccgt cctgcacgcg cacgtcatgc ggctgcccgt cggcgctgac gccgggaagg
    15061 agcagtgcaa cccctgcgac gacggcgccg acgatccacg ccagcgcgag cggcccgagc
    15121 gccgccagcc gatcacgatc gagccagagc cagacgattg cggcgacgac cgtcagcacg
    15181 atccccacgc gcgagtacgt caggaccgcg acaactagcg ccgcgtacaa cagcagcacg
    15241 ccgcccatcc gggcccgccg atcgtgtcta ggcccggccg cccacagccc gagcggcact
    15301 gaggcagccg cgagcaacgc gagctcgttc cagtactcga tcggatagcg caaacgagcc
    15361 agccgctcgt agtccggata gagcccggga atcaccttcg ccgccagagc ccagaagaac
    15421 agcgccgcga gtagcaaaca cccagcccac gcgaaacggc gcagcggcgt gcccgcgacg
    15481 agcgccccga ccgcggcgaa cgcgaagtag acgagcccgc ggttcgtgta gtcccacgag
    15541 cggctcggct cgatcgacca cgcgatcgac accccttgcc agacgacgaa cgccgccagg
    15601 gacaggaaga agatggagcc ccggagcggc aggcgcggtg gccgccacgc ccagccgacc
    15661 gccgccacca gaacggccgg cgctccgatc cagaagaccc gggactggct cgacccctca
    15721 ccgaagaaga gcgccgcaaa ggacagcccg ccggccgcgg ctccccaggc aacgggccag
    15781 cgcatcgcgc gcgaagctaa cagagccgca ggccgcatcc acgaagccct gtcccacatt
    15841 cgaacaccgc cggaaccgag ccacagctac cttacaaagc gcttacaact gactcatggc
    15901 acccacactc ccatccggca gggtgggcaa gatcgtcgtt agagtctcgc gcaagccgca
    15961 gccggcaccc gcatgccccg attcctgaag cccctcattc tcgtcgctgc agccgtggcc
    16021 atcggcgccg cagcgctcgg cacggcgcag ccggcgcagg cgcgatcgat gagtacctgc
    16081 tcgagcgcgc tgatccacga ctggtacgtc gacggccgcg tcgacaagac gtacccggtg
    16141 cactgctacc gcgaggcgct gaacgagatc ccggaggacc aggccatcta cgggacgctc
    16201 cgccaggacc tgacgcgcgc ccttcaagcg acgattcgcc agcacggcgg ccacgtgacc
    16261 ggcgacacgc ttgtcgtacc gctgggcggc ggcggcggca acggcccgaa cggaaccggc
    16321 agctcgggcg gcatcttcca ctggctcgcg cagaagctcg gcccgagcac ggcggactcg
    16381 attccggtcc cgctgctcat tctcggtggg ctcgccttcg cattgatggc ggccgccggt
    16441 gtgagcttga tcgcgcgacg gatgcaggcc cgtcgggcgg cggcgaatcc gccgcccgca
    16501 ggcccctcct tctagacgct caggcgcgtg cgcgcgcggg tcgtcagacc cgttgcgacg
    16561 agggtcagcc cgagcaggac gaacagcagc aggccgagcc ccgtgaacgg gagctggccc
    16621 agcgtctgcg gtccgccggt cggcgtcttc gtggcgccga acgggttgcg attcgggcgc
    16681 ggcgcgccct cggacgtacg cgtgcccggc gccgacggca ctccggttgc tgacgccgga
    16741 gtcgacgccg acgctgccgg agtcgaagcc gccgttgcgg tcggacccga agcaaccgac
    16801 ggcgttgcac cgaccggccc ggactgaacc gtcccggtcg agccggtggc cgtgttccca
    16861 ccaccgacct gtgcggtgcc gatcgaatcg gtggcggagt tggtcccacc accgccgccg
    16921 accgtcacgg gcgccgacgt cgtcgtgccg cccgcccctg cagtcgttcc ggccgtcact
    16981 gcaccggact gcgtcgcgag gaacgaacgc gtgaccgtgt tgccgccgcc gacctgagct
    17041 gctccgaccg cgtcggccgc gctgttgcca ctacctgcga cccccaccgg cgcggaggcg
    17101 gagaccgcct cgccgacgag cgacgcaccg acggcgggac tcgcctgcgg cgagccggac
    17161 tgcagcactc cggtcgagtc gccggacgag ttgcccccgc cgacctgagc agcaccggtc
    17221 gagctcgacg cgttgttgct gccgtcaccg ccgaccgtca cggggacacc ggcgctcgcg
    17281 ctcgagcccg atgcgccggc ggacgccgac ggcgacgcac tcacggagct gacctgcacg
    17341 ctgccggtgg agtcgttcga ggtgttgcca ccaccggcct gcacagcgcc cacggagttc
    17401 gtcgccgtgt tgttgcctga gccacccact gcaaccggga cggaagcggc gacagttgcg
    17461 cccgcctgac tagcgtccgc ggtcggcgtg actccgaccg ggccggtctg gactgctccg
    17521 accgagccgg tcgcgtcgtt gtcggccagc gcggtttgcg ctgtgccgag cgtgagcgcc
    17581 acgactgcgg cgatcacgag caagcacctt gctctcatgt tcccttcttt cctagccatc
    17641 gttcgagttg actgcgcctc tcggcagcac ttgcgcggaa tcactcgatc gagggacggc
    17701 atgtgaacta cctcacgtag gtcacagtga ggacgaagga cgtcttcgct ccggtcccgc
    17761 cgacccgcag agtgaacgtc cccgccgcgg acgttccgtt cagctggatc gggctattcc
    17821 ccgtcgctcg cccggttggg gtgaccaggg tgagccgctt gccgcccgtg aaacgaagtg
    17881 tcgcgctgaa cgcgccggcg ccgacgtcga cgttgtaggt gcgcgcgcgt acgcgactcc
    17941 ctagcgttcc cctgaagacc gcgctggtcg ccgcggccgg acgcagcagc gccagcgtcc
    18001 tgcccgcgtc gatgcggccg tactggacga acgccggcag cgggaccgct gcgcgcacaa
    18061 gtgcgtcctc cgcttcacgc ggggtcgcgg caggctgcgc gctcagctcg agcgcgacga
    18121 gacccgccac gaccggcgtc gccgacgacg tgccgcagaa ggtgccgtac ccgccgccga
    18181 gagtcggtgc gatgttgcag cccggagctg cgagccgcac ccacgagccg aagttcgacc
    18241 acgagtagcg accgtcggcc accgtcgtcg cggcgacgcc gatcgcatgc ggatccgcag
    18301 ccgggtagaa ctgcgtcgtg gtgcccgcgt tgccggcggc cgcgacaacg accacgccct
    18361 tcgcgctcgc a
//
LOCUS       DASVEU010000226         8577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_354008,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000226 DASVEU010000000
VERSION     DASVEU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354008"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1125)
                     /locus_tag="VF225_09795"
     CDS             complement(127..1125)
                     /locus_tag="VF225_09795"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7145584.1"
                     /translation="MSSPQTTASDLTAVAEAIREHDRFVLTTHENPDGDALGSLLAAN
                     LALEQLGKDTVMVLHGDAPLPGEYGFMPLGDLQRRWPDDVAERVLLAVDCANESRIAD
                     PEVLGRVPLSINVDHHHDNTRFGLINLVVADASSTGEVLRDLFRELGVELTPDIAEAL
                     YIALVTDTGRFQYTNTTPKALRLAAELVEAGADVHRVFQGVYESVQFAKLKLLARALE
                     RAQVFEGGRIVVSYLLRTDFTEVGAAEPYSEGIIDFLRAVEGADMAVLIREPPRQDGP
                     ARRVSLRASIDELDVSAIARKSDGGGHRQAAGFSSEASIEEITDFVRREFLASSRA"
     gene            complement(1097..1444)
                     /gene="rbfA"
                     /locus_tag="VF225_09800"
     CDS             complement(1097..1444)
                     /gene="rbfA"
                     /locus_tag="VF225_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7145585.1"
                     /translation="MTDRMRRVNESVRQVLSEAIGQLKDPRIGFVTITGVETSTDLRH
                     ARVYLSVLGAERKRAQTLAGLQAARSLLQAQLGRELRLKRTPQLAFEYDPSVERGVRM
                     TKLIDELAPDDSE"
     gene            1462..2211
                     /locus_tag="VF225_09805"
     CDS             1462..2211
                     /locus_tag="VF225_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7145586.1"
                     /translation="MHALLLVVVGLWCFLTALMGGLVGLVLGNIRLPVLVLAASSPAA
                     GAGANIGVSAVAALAAAVAHIRAGRIDWRLFAWMAPPSVIGAVVGGLLSGVVPGNALL
                     VVIGVTLLVFGIDLLRPKAGWRNAAPGRVNVRAAILSGAVIGLLGGFVGLILGALRLP
                     ALLRFVGSDPARAVGTNAAVGFCLGVGGVFGHLREGVDWWLLVVGAATSVPGAILGAR
                     LTGRLDERQLLRAIGLVLLVAGSAMILQGVV"
     gene            complement(2208..2504)
                     /locus_tag="VF225_09810"
     CDS             complement(2208..2504)
                     /locus_tag="VF225_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX7145587.1"
                     /translation="MGAGYIGILSVELHFPEAGSLKGKRKHVKSAKAQLQNRFGASVA
                     EVDHHELWQRARLTLSCVAREHREAERLLDAAERYLAAQEFELVRADRDVVTLD"
     gene            complement(2524..4509)
                     /gene="infB"
                     /locus_tag="VF225_09815"
     CDS             complement(2524..4509)
                     /gene="infB"
                     /locus_tag="VF225_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7145588.1"
                     /translation="MANESGSNRPNRPMRPRQGGAGQRRKRRVVIDNAAARPRDARQQ
                     QREREASRPKTPREVVAPTGPITVPSGVTVRDLSQALGVPAPKIIMFMMGLGKAVTIT
                     QTLADDEVQLIAAELAPEREFTIKHAADEDEEPESYEDSAADLAPRPPVVTIMGHVDH
                     GKTTLLDAIRKTSVVATEAGGITQHIGAYQAEHNGRRITFLDTPGHEAFTAMRARGAK
                     VTDIAVLVVAADDGVMPQTKESISHARAADVPIVVAVNKIDVPDSDPNRVRSELSTEG
                     VQPEEWGGTPQFSDVSAREAQNLDDLLEKVLLVADAELELTANPTAEASGPIIESRLD
                     VGRGPVATMLIHRGTLHVGDAIVAGDAWGKVRALYNFRGDKVKDARPGDPVEILGFDK
                     PPPAGELGRVVENERRARELANARGERLRREQLAQGSRGVSMESLFEQIEAGAVQDLN
                     LVLKGDVVGSVEAAVSELGKIQHSEVRVNVIHQGVGGITEGDIMLASASGATVVGFNV
                     RPNAEARSLAEREGVEIRTYRVIYQLTEDIEQALVGMLKPVEKEETLGEAEVRALFKV
                     SRLGTIAGCMVTSGVIRRSAQARVIRDGTVIIETTISQLKRFKDDAREVAEGFECGIL
                     LDGFNDVKEGDIIEAFETREIERTTLDQPPAAAASTS"
     gene            complement(4750..6243)
                     /gene="nusA"
                     /locus_tag="VF225_09820"
     CDS             complement(4750..6243)
                     /gene="nusA"
                     /locus_tag="VF225_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7145589.1"
                     /translation="MSQEIVEAVRDIEREKGIESGTLVAALEDALLAAYKKMPGAARH
                     AIVEIDELGDFRVFAVELPADIEGRLIEEARERKLEELEALEVETGERSHTLISDDDL
                     DVDWSDVPEHLIKREDVTPENFGRIAAQTAKQVILQRIREAERAMMYDEYVDRVTEVV
                     TGIVQQAGDRNNVLVDLGKVEALLPRSEQVDGERYEQGSRIKAVITEVRSGTKGPQVI
                     LSRRDPELIKTLFELEVPEIADGLVEIRGVAREPGYRSKIAVESHAQGVDPVGACVGP
                     RGSRVRMVVSELRGEKIDIIPWNPEPARFVAKALSPARVREVFVDDETKEATVIVPDD
                     QLALAIGKEGLNARLAARLTGWKVDIQSDTEFAQAEAAAAYGGANEGEEFTGRCSAVL
                     SNGKRCPNEALPGSRYCGLPAHQALALQDGNGAESADVPAVADDAAVAAVVDDLVAEV
                     EDDGAPELAAVEPDVVPQLADAEQVPVGIPVEETSAPGDTEEHPPAA"
     gene            complement(6240..6677)
                     /locus_tag="VF225_09825"
     CDS             complement(6240..6677)
                     /locus_tag="VF225_09825"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145590.1"
                     /translation="MAGTHDKEKQLTREIAPKVESAIPGTEVLAVELTSPERFTVFVD
                     HVQGVDHALCERVTNVLRDYLREYSVDVSSPGLERPVRKPDHFRAAVGRRVALRTRER
                     NRVRGEVVAADERAVTVKTQAESVDIPYDAIVRGNLIEEAGKR"
     gene            complement(6833..7267)
                     /gene="dtd"
                     /locus_tag="VF225_09830"
     CDS             complement(6833..7267)
                     /gene="dtd"
                     /locus_tag="VF225_09830"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX7145591.1"
                     /translation="MRAVCQRVSEARVTVDGVVTGEIGAGLAVFVGIERTDEARDARE
                     LARKVAQLRIFEDDRGKFAQSLLDTSGSVLVVSQFTLVANTDKGNRPSFTNAAPPEEA
                     EPLYEEFCAALAAEGTEVETGVFGARMAVALVNEGPVTIVLG"
     gene            complement(7421..7837)
                     /locus_tag="VF225_09835"
     CDS             complement(7421..7837)
                     /locus_tag="VF225_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7145592.1"
                     /translation="MSETYDLYQQGRDHLKKGRAAQATVALEKAKRREPEKASIREAL
                     GIAYLRIQRYEEAEQEFRALLELSPADHYAHYGLGRALEKQGKQLEANGHYKLASSLR
                     PQNEHYASRILDLDQEGDEPNESGDSEAPGADSAGN"
     gene            complement(7834..>8577)
                     /locus_tag="VF225_09840"
     CDS             complement(7834..>8577)
                     /locus_tag="VF225_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7145593.1"
                     /translation="GVSCPSVHSGSVREAMDAVVMAAGEGTRLRPLTEQWPKPVLPID
                     GRPVLAVLLRELAAANLRHVWVVIGHLAEQVEELAGDGSAFGLELSFARQPGVLGSAD
                     AVQRALDAGAKTPLLVSAADTVYRRGDVGQFAAEFSRSGAAGSVAIRTDPPPGPGRQA
                     VRRSGARIESLHDEDPDNPWSAAPLWALGSAVAERLCLDNPPYELEHAFQAAIEAGET
                     VTAVEIGKTRDLTYPLDLVKENFPYLRAT"
BASE COUNT         1270 a         2963 c         2810 g         1534 t
ORIGIN      
        1 ccagtcgcaa aagggtcaag agttccggca tggcccgtgc gtgcgccagt ccggcggcgc
       61 agatcggaga cgatcgcaaa cgaggacggc ccgccgggct tgtccacgag gacgattccc
      121 gccggactaa gcgcgcgagg aggcaaggaa ctcccggcgc acgaagtccg tgatctcctc
      181 gatcgacgcc tcgctggaga aacccgcggc ctgacgatgc ccacctccgt ccgacttgcg
      241 ggcgattgcg gaaacgtcga gctcgtcgat gctcgcgcgc aggctgacgc gtctggccgg
      301 gccgtcctgg cgcgggggct cgcggatgag aacggccatg tccgctccct cgactgcacg
      361 gaggaagtcg atgatccctt ccgagtacgg ctccgcggcc ccgacttcgg tgaagtccgt
      421 tcgcaagaga tacgagacga cgatgcgtcc accttcgaag acctgggcac gctcgagcgc
      481 gcgcgcaagc agcttgagct tcgcgaactg caccgactcg tagacgccct ggaacacgcg
      541 gtggacatcg gctccggcct cgaccagctc ggcggccagc cgcagggcct tgggggtcgt
      601 gttcgtgtac tgaaaccggc ccgtatcggt gacgagcgcg atgtagaggg cctcggcgat
      661 gtccggtgtc agctcgacgc cgagctcgcg gaagagatcg cgcagcacct cgccggtcga
      721 agacgcatcg gcgacgacca ggttgatcag gccgaaccgc gtgttgtcgt ggtgatgatc
      781 gacgttgatc gagagcggga cacgcccgag gacctccggg tcggctatcc ggctctcgtt
      841 cgcgcaatcg acggcgagga ggacacgctc cgcaacatcg tcgggccagc gccgctgtag
      901 gtcacccagc ggcatgaacc cgtactcgcc tggaagcggc gcgtcgccgt gcagcaccat
      961 gacggtgtcc ttgcccaact gctccagggc gaggttcgcg gcaagcagcg agccgagagc
     1021 gtcgccgtcg gggttttcgt gcgtcgtgag gacgaaccgg tcgtgttcgc gaatcgcctc
     1081 cgcgaccgcg gtcaggtcac tcgctgtcgt ctggggcgag ctcatcgatc agctttgtca
     1141 tgcggacccc acgttcgacc gacggatcgt actcgaacgc cagttgagga gttcgcttca
     1201 ggcgcaactc gcgcccgagc tgcgcctgca acaaagagcg ggccgcctgt aggccggcga
     1261 gtgtctgcgc acgctttcgc tcggcaccga ggacggacag gtagacccgt gcgtggcgga
     1321 gatcggtcga ggtctctacc ccggtgatcg tgacgaagcc gatccgaggg tccttcagtt
     1381 gcccgatcgc ttccgacagg acttggcgga ccgactcgtt gacgcggcgc attcgatctg
     1441 tcatacggga tattcaagcg cttgcacgct ctcctcctcg tcgtggtcgg gctctggtgc
     1501 ttcctcaccg cgctcatggg tggcctcgtg ggcctcgtgc tcgggaatat ccggctcccg
     1561 gtgctcgtcc tcgccgcctc gagcccggcg gcgggcgccg gcgcgaatat cggcgtctcg
     1621 gccgtcgccg ccctcgctgc agcggtcgcc cacattcggg caggccggat cgactggcga
     1681 ctgttcgcct ggatggcgcc gccctcagtc atcggggccg tagtcggagg tctcctgtct
     1741 ggtgtcgtcc cgggaaacgc tctgctcgtc gtgatcggcg tgacgctgct cgtcttcggg
     1801 atcgacctgc ttcgcccgaa ggccggctgg cggaacgccg ccccggggcg ggtcaacgta
     1861 cgagccgcga tcttgagcgg agcagtgatc gggctgctcg gcggtttcgt cgggctcatt
     1921 ctcggcgcgc tgaggctccc agcgctgctt cgcttcgtcg gctcggaccc ggctcgcgcc
     1981 gtaggcacga atgcagccgt cggtttctgt ctcggcgtgg gcggcgtctt cggccacctg
     2041 cgggaaggcg tcgactggtg gctgctcgtc gtgggcgcgg cgacgtcggt gccgggcgcg
     2101 atcctcggcg cacggctcac cggtcgcctg gatgagcgcc agctgctccg ggcgatcgga
     2161 ctcgtcttgc tcgtggccgg tagcgccatg atccttcagg gcgttgtcta gtcgagcgtg
     2221 accacgtcgc ggtcggcgcg cacgagctcg aattcctgcg ctgccaggta gcgctcggcc
     2281 gcgtcgagca gccgctccgc ttcgcgatgc tctctcgcga cgcaggagag ggtgagccgc
     2341 gcccgctgcc acagctcatg atgatcgact tctgcgaccg aggccccgaa gcgattttgc
     2401 aactgggcct tcgcagattt gacgtgcttc cgcttcccct tcagcgaccc ggcctccggg
     2461 aaatgcagtt cgacgctcag aatgccgatg tagcccgcgc ccatgtctcc gggctctgat
     2521 cggctaggaa gtggaggcgg ccgcagccgg cggctggtcg agcgtcgtcc gctcgatctc
     2581 acgtgtctcg aaggcctcga tgatgtcgcc ctctttgacg tcgttgaagc cgtcgagcag
     2641 gattccgcac tcgaagcctt cggcgacctc gcgcgcgtcg tccttgaacc gcttgagctg
     2701 agagatcgtc gtctcgatga tgacggtgcc gtcgcggatg acgcgcgcct gcgcggagcg
     2761 gcgaatgaca ccgcttgtca ccatgcagcc ggcaatcgtc ccgagccgcg agaccttgaa
     2821 gagcgcacgt acttctgctt cgccgagcgt ctcttccttc tccaccggct tcagcattcc
     2881 cacgagcgcc tgctcgatgt cctcggtcag ctggtagatg acacggtacg tgcgaatctc
     2941 gacgccttcg cgctcggcga gcgagcgtgc ttcggcattc ggtcggacgt tgaatccgac
     3001 caccgtggca ccggaggccg acgcgagcat gatgtcgccc tcggtgatgc cgccgacacc
     3061 ctggtggatg acgttcacgc gcacctccga gtgctggatc ttgccgagct cggaaaccgc
     3121 cgcttcgaca gagccgacca cgtcgccctt gaggacgagg ttgagatcct ggaccgcgcc
     3181 tgcctcgatc tgctcgaaga ggctctccat cgagacgccc ctcgacccct gagcgagctg
     3241 ttcgcggcgc agcctctcac cgcgcgcgtt tgcaagctcg cgcgcacgcc gctcgttctc
     3301 gaccacacgt cccagctcac cggcgggcgg cggcttgtcg aacccgagga tctcgacggg
     3361 atcgcccggc cgtgcgtcct tcaccttgtc cccgcggaag ttgtagaggg cacgcacctt
     3421 cccccaggcg tcgccggcga cgatcgcatc tccgacgtgc agcgtgccgc ggtggatgag
     3481 catcgtcgcc actgggccgc gaccgacgtc gagacgcgac tcgatgatcg ggccggacgc
     3541 ctcggcggtg ggattcgcgg tcagctcgag ctcggcgtct gcgacaagca acactttctc
     3601 gaggaggtcg tcgaggttct gggcctctcg ggccgagacg tccgagaact gcggcgtccc
     3661 tccccactcc tccggctgca cgccttcagt cgacagttcg ctgcgcacgc ggttcggatc
     3721 ggagtccgga acgtcgatct tgttcaccgc gacgacgatc gggacgtcgg ctgcgcgcgc
     3781 gtgggagatc gactccttcg tctgcggcat gacgccgtcg tcggccgcga ccacgagcac
     3841 cgcgatgtcc gtgaccttcg cccctcgcgc acgcatcgca gtgaaggctt cgtggcccgg
     3901 cgtgtccagg aaggtgatcc tgcggccgtt gtgctcggcc tggtacgcac cgatgtgctg
     3961 cgtgatccca ccggcttcgg tggcgacgac ggaggtcttg cggatcgcat cgaggagcgt
     4021 ggtcttgccg tggtcgacgt gacccatgat cgtgaccacg ggagggcgcg gcgcaagatc
     4081 ggccgcgctg tcctcatagc tctcgggctc ctcgtcttcg tccgccgcat gcttgatcgt
     4141 gaactcgcgc tcaggcgcga gctcggcggc gatcagctgg acctcatcgt cggccaaggt
     4201 ctgagtgatc gtcacggcct tccccagccc catcatgaac atgatgatct tgggcgccgg
     4261 cactccgagg gcctgcgaaa gatcccgaac cgtgacgccc gacggcactg tgatcggccc
     4321 ggtcggggcg accacctcgc gtggcgtctt gggccgcgac gcctcgcgct cgcgctgctg
     4381 ctgccgcgcg tcgcgggggc gtgcagccgc gttgtcgatc acgacacggc gcttgcgtcg
     4441 ctggcctgcg ccgccctggc gagggcgcat cggccggttg ggtctattcg agcctgattc
     4501 gttggccatc agatgttcaa gtgtagaggg atgacagttt ctgatcgatt tggacggtct
     4561 gccgcagcgt ccggttgaac gcgcggcgcg agactgcccg ctcgaagcaa gcgagccgac
     4621 ggcaggtata ggccccgcga ccgtgaatcc ccgtccccgg caccagctgc ccggccgccg
     4681 ccgcgaaacg caggagctcg ccctgggcag ctttccgccc gcagccgacg cagcttctga
     4741 tcggtccggt cacgcagctg ggggatgctc ctcggtgtcg ccaggcgccg atgtctcttc
     4801 gaccgggata cccaccggca cctgctcggc gtccgcgagc tgcggaacca catcgggctc
     4861 gaccgccgcg agctctggtg cgccgtcgtc ttcgacctcc gcgacgagat cgtcaaccac
     4921 ggctgcaacg gctgcgtcgt cggccaccgc cggtacgtcg gcactctcag cgccgttgcc
     4981 gtcctggagt gcgagcgcct ggtgcgcagg cagcccgcag taacgtgagc cggggagcgc
     5041 ctcgttcggg caacgcttgc cgttggagag caccgccgag caacgaccgg tgaactcctc
     5101 gccctcgttg gctccgccgt atgccgcagc ggcttccgcc tgagcgaatt ccgtatcgga
     5161 ctggatgtcg accttccagc cggtcagccg cgcggccaga cgggcgttga gaccttcctt
     5221 gccgattgcg agcgcgagct ggtcatccgg aacgatgacg gtcgcctcct tggtctcgtc
     5281 gtcgacgaag acctcgcgca cacgtgcggg agacagcgcc ttcgcaacga atcgcgccgg
     5341 ctctgggttc cacggaatga tgtcgatctt ctcgccgcgg agctccgaca cgaccatgcg
     5401 cacgcgcgag cctcgtgggc cgacgcaggc gccaaccggg tcgacgccct gggcgtgcga
     5461 ctcgacggcg atcttcgacc ggtacccggg ctcgcgggct acgccgcgga tctcgacgag
     5521 cccgtcggcg atctccggca cctcgagctc gaagagggtc ttgatcaact cgggatcgcg
     5581 acgcgacagg atcacctgcg gtcccttcgt gcccgatcgg acctccgtga tgacggcctt
     5641 gatgcgcgag ccctgctcat agcgctcgcc gtcgacttgc tccgaacgcg gcagcagtgc
     5701 ctcgaccttt ccgaggtcca cgagcacgtt gttgcggtcg cccgcctgct ggacgattcc
     5761 cgtcacgacc tcggtcacgc ggtcgacgta ctcgtcgtac atcatcgcgc gttcggcctc
     5821 gcggatccgc tgcaggatca cctgcttcgc agtctgcgcg gcgatgcgcc cgaagttttc
     5881 gggagtcacg tcctcgcgct tgatcaggtg ctcgggcaca tcggaccagt cgacgtcgag
     5941 gtcgtcgtcg gagatgagcg tgtgcgagcg ctcgcccgtc tccacctcga gcgcctcgag
     6001 ctcctcgagc ttgcgctcgc gcgcttcttc gatcagacgc ccctcgatgt ccgcgggcaa
     6061 ctccaccgca aagacgcgga aatcacccag ctcgtcgatt tcgacgatcg catggcgcgc
     6121 ggctcccggc atcttcttgt acgcggcaag gagggcatcc tcgagcgccg cgacgagcgt
     6181 gccgctctcg attcccttct cccgctcgat gtctcgaacg gcctcgacga tttcctggct
     6241 catctcttcc ccgcttcctc gatcagattg ccccgcacga tcgcgtcata cgggatgtcc
     6301 acggactcgg cttgcgtctt cacggtcacg gcgcgctcgt ccgctgcgac gacctcaccg
     6361 cgcactcggt ttcgctctcg ggtgcgtagc gcgacgcggc gacccacggc ggcgcgaaaa
     6421 tggtcgggct tccggaccgg tcgttcgaga cccggcgacg agacgtcgac ggagtactcg
     6481 cggaggtaat cccgcagcac gttcgtgacg cgctcgcaga gcgcatggtc gacgccctgg
     6541 acgtgatcca cgaacaccgt gaaccgctca ggactggtga gctcgaccgc gagcacctca
     6601 gtgcccggaa tcgcgctctc gaccttcggt gctatctccc gcgtcagctg cttctccttg
     6661 tcgtgtgtgc ctgccatcgc ccttgctccc gtcacaaaaa atgggcccgc aggcccactt
     6721 cgcgaacagc tcgaaatgtg gtgttagcag gatagcaagc cattcggagc cgagcacgag
     6781 cgattttccc tcttcttgct cgggaaacgc ctctccacgc ggggtgccgg ggtcagccga
     6841 ggacgatcgt caccgggcct tcattcacga gcgcgaccgc cattcgggcg ccgaacacgc
     6901 ccgtttcgac ctcggtgcct tccgcggcga gggctgcgca gaactcctcg tagagcggct
     6961 cggcctcttc aggcggggcg gcgttcgtga agctcgggcg gttgcccttg tcggtattcg
     7021 cgacgagtgt gaactggctg acgaccagca ccgatcccga cgtgtcgagc aaactctggg
     7081 cgaacttgcc gcgatcgtcc tcgaagatcc gtagctgcgc caccttccga gccaattccc
     7141 tcgcgtcgcg ggcctcgtcc gtgcgttcga tccccacgaa gacggcgagg ccggcgccga
     7201 tctcgccggt cacaacgccg tcgacggtga ctcgggcttc ggacacccgc tgacaaaccg
     7261 cccgcatcgg gcgcccatca tctcagccac gcgggcagga catccagtta cacgcacgcg
     7321 cgcgtcccca cccgggtggg ggtgtcaccc accacccact gcgttcgacg ctatcgccga
     7381 tgtgtgcgca gctcccgcgc gaacgtgcgc cggatctgcg tcaattgccg gcgctgtcgg
     7441 cgccgggcgc ctcggagtcg ccggattcgt tgggctcgtc gccctcctgg tcgaggtcga
     7501 ggatgcggga ggcgtaatgc tcgttctgcg ggcgcaagga gctcgcgagc ttgtagtggc
     7561 cgttggcctc cagctgtttg ccctgcttct cgagcgcacg tccaagcccg taatgcgcat
     7621 agtggtccgc cggtgagagc tcgagcagcg cgcgaaattc ctgttccgct tcttcgtagc
     7681 gctgaatccg caggtaggcg attccgagcg cttcgcgaat cgacgccttc tccggctcgc
     7741 gccgtttggc cttctcgagg gcgacggtcg cctgagccgc ccggcccttt ttcaagtggt
     7801 cgcgtccttg ctggtagaga tcgtaggttt cgctcatgtc gctcgcaggt agggaaaatt
     7861 ctccttcacc aagtcaagcg gatacgtcaa atcgcgtgtc ttcccgatct ccacagcggt
     7921 aaccgtctcg cccgcttcga tcgcggcctg gaacgcgtgc tccagctcgt agggagggtt
     7981 gtcgagacag aggcgctcgg cgacggccga cccgagcgcc cacagcggtg cggccgacca
     8041 cggattgtcc gggtcttcgt catgaaggga ctcaatccgg gccccgctcc ggcggacggc
     8101 ctgacggccc ggccccggag gcgggtcggt gcggatcgcc accgacccgg ctgcaccgga
     8161 tcggctgaac tcggctgcga actggccaac atcgccgcga cggtagacgg tgtctgcagc
     8221 cgagacgagc agcggcgtct tcgcgccggc gtcgagtgcg cgctggacag cgtccgccga
     8281 gccgagcacg ccgggctggc gcgcgaagct cagctcgagc ccgaacgccg aaccgtcgcc
     8341 cgcaagttcc tcgacctgct ccgccaagtg cccgatcaca acccagacgt gccggaggtt
     8401 tgccgccgcg agctcccgga ggagaacggc gagcaccggc cgcccgtcga tcggcagcac
     8461 aggcttcggc cactgctcgg tgagcgggcg gagtcgcgtt ccctcccccg ccgccatgac
     8521 gacggcatcc atggcctctc taaccgagcc cgagtgaacg gacggacagg agacccc
//
LOCUS       DASVEU010000227         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1325768,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000227 DASVEU010000000
VERSION     DASVEU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325768"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VF225_09845"
     CDS             complement(<1..398)
                     /locus_tag="VF225_09845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX7145594.1"
                     /translation="MSFLAFIPSPSSGTIDLGPLTIHMYGLMLLLAIAAACALTGYRW
                     VSWGGDWDLVFRVAVWGVAAGIVGARLYHDVTSWNEVPDKWWGPFAVWQGGLGVWGGI
                     LFGVLAGAWIIHRSGQSVRLFMDAVAPGLL"
     gene            complement(438..635)
                     /locus_tag="VF225_09850"
     CDS             complement(438..635)
                     /locus_tag="VF225_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145595.1"
                     /translation="MTVAETEIREEIELVEAWRAEQLETAGYGAQTAAEIAMRQDIDL
                     HLAIDLLSSGCSAELAAQILL"
     gene            complement(676..1371)
                     /locus_tag="VF225_09855"
     CDS             complement(676..1371)
                     /locus_tag="VF225_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145596.1"
                     /translation="MTELERALVALGRELDIPPEPDLRSRVRERIERRVRYRRAAVLA
                     LAVLVVAVGIALAVPPARSAILRFFHIGSVTIERVDTLPAARERSLVAGLGPALSLRH
                     AEKSSGVPLVLNAPKPRRFYAQPGLIAAVLHYRGKSVLLAEFQGDQMGLTKKFVSPGT
                     SVEPAPIGSFGVWLEGGKHVLTWQGASGEIRQMERRLAGNVLIWAQGNRTYRLEGSLN
                     KAEMLELGRQITR"
     gene            complement(1364..1912)
                     /locus_tag="VF225_09860"
     CDS             complement(1364..1912)
                     /locus_tag="VF225_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145597.1"
                     /translation="MVGRPLDESDLIGRAQRGDTHAYEELVYAHQGIAFRTAYVIAGN
                     AADAEDAAQDAFVKAWRALGRFREGAPFRPWLLQIVANEARNRRRSAGRRAHLALRAA
                     TEQPSGDAAPSPEASLLSAELRDALLAAVNELPEDQRTVIALRFFAGLSEHEVAEALG
                     LPEGTVKSRSARALERLRESYD"
     gene            1981..2418
                     /locus_tag="VF225_09865"
     CDS             1981..2418
                     /locus_tag="VF225_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145598.1"
                     /translation="MPSLVGINHIALEVDDIDEALAFWESIFGPLQLRGRGKQMAFID
                     MGDQFIALARGRTQPPDQHRHFGLVVDNKEAVRARLRELGIPVSSGRGLDVRDPSGNL
                     IQIVDYRDVQFTKTDNVLSAMGLNDIEKSESALAELRAKGIDV"
     gene            2557..3549
                     /locus_tag="VF225_09870"
     CDS             2557..3549
                     /locus_tag="VF225_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7145599.1"
                     /translation="MSGSTDAAIDVASRFLENLESVIRGKHEEIKLVLAALACKGHVL
                     FEDVPGTAKTVLARAIARSVEGAKHARIQCTPDLQPTDVTGLSVYNQHTRDFEFRPGP
                     IFANTVLVDEINRAMPKTQSALLEAMAEQQVTVDGVTRPLIQPFILLATENPVEYEGT
                     FPLPEAQLDRFFVKTALGYPSLEDEFQIVLDQQAEHHPLAGLGSVVSTAEVTQLQDAV
                     RAVYVDELLRRWIVELVRSTREVEMCAIGASVRGTLALERAARAWALLDGREFVVSDD
                     VEHLFLPVIAHRVGFRPAFRAQARRTGWDDALADFRDACLRLVPAPEVRRGQPA"
     gene            3568..4476
                     /locus_tag="VF225_09875"
     CDS             3568..4476
                     /locus_tag="VF225_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7145600.1"
                     /translation="MTFELVPRGRIVGLAFGAMRSARRGAGSDVAGSRRYVPGDDVKA
                     IDWGASARLSSARSSDDFILRERFAEEAPRVVVICDRRPEMQLFPRGFPWLRKVDAQR
                     AAIELIRASAIEAHGFLGYLDFGESGDEPFWLPPQSQSLWEYQDREAEGAPFRAPESN
                     VEHAFDFLMSHPRSVPAGTFVFVLSDFLVPPPAETWLRALEQRWDVIPVVLQDPLWER
                     SFPDLDGFVVELADVGGHSQHVRLRRREVAERRRANETRWEKLVDDFARASLEPVVID
                     SSEESEILRAFLDWAETRQEGGGGAW"
BASE COUNT          711 a         1582 c         1513 g          720 t
ORIGIN      
        1 aacagaagcc ccggtgcgac ggcgtccatg aacaaccgca cgctctggcc cgagcggtgg
       61 atgatccagg ctccggcgag gacgccgaag aggattccgc cccagacgcc gagcccgccc
      121 tgccagaccg cgaacggccc ccaccacttg tcgggcacct cgttccagct cgtgacgtcg
      181 tggtagaggc gcgcgcccac gatcccggcg gcgacccccc acacggcgac gcggaacacg
      241 agatcccagt cgccccccca gctgacccac cggtatcccg tcagcgcgca ggcggccgcg
      301 atcgcgagca ggagcatgag cccgtacatg tggatcgtga gcggcccgag gtcgatcgtg
      361 ccgctcgacg gcgacggaat aaatgcaagg aagctcaccg gcaggagatt ctgccggtag
      421 ccgtcgtcac ctgcttgtta gagcaggatc tgagcagcaa gctcggccga gcagcccgac
      481 gagagcagat cgatcgcgag gtgcaggtcg atgtcctgcc gcatcgcgat ctccgccgcc
      541 gtctgggcgc cgtagcccgc cgtctcgagc tgctcggccc gccaggcctc gaccagctcg
      601 atctcttccc tgatctccgt ttccgccaca gtcatgcttc tctattcgga cgaagcggct
      661 ctgtggtttg tccccttacc gggtgatctg tcgtcctagc tcgagcattt cggccttgtt
      721 caagctgccc tcgaggcgat aggtgcggtt gccttgggcc cagatgagga cgtttccggc
      781 cagccgacgc tccatttgcc ggatctcccc cgatgcccct tgccaggtca ggacgtgctt
      841 gccgccctcg agccagacgc cgaacgagcc gatcggcgcc ggttcgacgc tggtgccggg
      901 cgagacgaac ttcttggtga gccccatctg gtcaccctga aactccgcga ggagcacgga
      961 cttcccgcgg tagtggagga cggctgcgat cagtccgggc tgtgcataga aacgccgcgg
     1021 cttaggtgcg ttcaggacca gcggcacgcc ggagctcttc tctgcgtggc gcaacgagag
     1081 cgccgggccg agccctgcaa cgagcgagcg ctcgcgcgca gccggcagcg tgtcgacccg
     1141 ctcgatcgtc accgagccga tgtggaagaa gcgcaggatc gcgctgcgcg caggtggcac
     1201 ggccagtgcg atcccgaccg cgacgacgag aaccgccagg gcgagtacgg ccgcgcgtcg
     1261 ataacgcacc ctccgttcaa tccgctcgcg aacgcgggag cgcaggtccg gctccggggg
     1321 gatgtcgagc tcgcgtccga gcgcgacgag tgcccgctcg agctcagtca taggactccc
     1381 tcagtcgttc gagcgcgcgg gcgctccggg acttcaccgt tccctcaggc aggcccagcg
     1441 cctcggccac ctcgtgctcc gacaggccgg cgaagaagcg gagcgcgatc accgttcgct
     1501 gatcctccgg cagctcgttc acggcggcga gcaaggcgtc gcgcagttct gccgagagca
     1561 gcgacgcctc gggggacggg gccgcgtccc ccgagggttg ctccgtcgcc gcccgcagcg
     1621 cgagatgcgc ccgccggccg gcagagcggc ggcggttgcg ggcctcgttg gcgacgatct
     1681 gcagcagcca gggacggaag ggcgcccctt cgcggaaccg cccgagcgca cgccacgctt
     1741 tgacgaatgc gtcctgcgcg gcgtcctccg cgtcggcggc attgccggcg atcacgtagg
     1801 cggtgcggaa ggcgatgccc tggtgtgcgt acacgagctc ctcgtaggcg tgagtgtcgc
     1861 cgcgctgcgc gcggccgatc aggtcggatt cgtcgagtgg tcggcccacc accgcctgct
     1921 tctacaccgt tcgcgcgtcc ggagttcccg cagcaggtgc tcgcggtcta cgctgaaccc
     1981 atgccgagcc tggtggggat caaccacatc gcgctcgagg tcgacgacat cgacgaggcg
     2041 ctcgccttct gggagtcgat cttcgggccg ttgcagctac ggggccgagg caagcagatg
     2101 gcgttcatcg acatgggcga ccagttcatc gcgctcgcgc ggggacgaac gcagccgccc
     2161 gaccagcacc gccacttcgg ccttgtcgtc gacaacaagg aagccgtgcg cgcacgactg
     2221 cgcgagcttg gcatcccggt gtcgtccggc cgcggcctcg acgtccgcga tccttccggc
     2281 aacctgatcc agatcgtcga ttaccgcgac gtccagttca cgaagacgga caacgtgctg
     2341 agcgcgatgg gtctcaacga catcgagaag tcggaatccg ccctcgcgga gctacgcgca
     2401 aaaggcatcg acgtctaaaa cagccggggt cagaccctgg cagttggaga cgtgcatctt
     2461 ggagtgacgg ctcgaacggc tggggtcaga cccttgcatt tcgggacttg tctctaggta
     2521 ttccccttcg agtatccgcg cgtatactcg ggcgggatga gcggctcgac cgacgcggcc
     2581 atcgacgtcg cctcccgctt cctcgagaac ctggagtcgg tcatccgtgg caagcacgag
     2641 gagatcaagc tcgtcctcgc cgcactcgcc tgcaagggcc acgtcctctt cgaagacgtg
     2701 cccggcaccg ccaagaccgt cctcgcacgg gcgatcgcac gcagcgtcga gggcgcgaag
     2761 cacgcgcgga tccagtgcac gccggatctg cagcccaccg acgtgacggg cctctccgtc
     2821 tacaaccagc acacgcgcga cttcgagttc cgccccgggc cgatcttcgc caacaccgtg
     2881 ctcgtcgacg agatcaaccg cgcgatgccc aagacgcagt ccgctctgct cgaggcgatg
     2941 gctgagcagc aggtcacggt ggacggcgtc acccggccgc tgatccagcc cttcatcctg
     3001 ctcgcgaccg agaaccccgt cgagtacgaa ggaacgttcc cgctgccgga agcgcagctc
     3061 gatcgcttct tcgtcaagac agcactcggc tatccgagcc tggaggacga gttccagatc
     3121 gtgctcgacc aacaggccga gcaccatccg ctcgccggcc tcggctcggt cgtcagtacg
     3181 gccgaggtca cgcaactgca ggacgctgtg cgcgcggtct acgtcgacga gctgcttcgc
     3241 cgctggatcg tcgagctcgt ccgctcgacc cgcgaggtcg agatgtgcgc catcggcgca
     3301 tcggtgcgcg gaacgctcgc gctcgaacgc gcggcgcgcg cctgggcgct cctcgatgga
     3361 cgcgagttcg tcgtctcgga cgatgtcgag catctcttcc tgccggtgat cgcgcatcgc
     3421 gtcgggttcc gccccgcctt tcgcgcgcag gcgcgcagga cgggctggga cgacgcgctc
     3481 gcggacttca gggacgcctg cctgcgactc gtccccgcgc cggaggtacg gcgcggccag
     3541 cccgcgtagg agctcctcct gcccgacgtg acgttcgagc tcgtcccgcg gggtcggatc
     3601 gtgggactcg cgttcggcgc gatgcgcagc gcacgccggg gtgccggctc ggacgtcgcc
     3661 ggctcgcggc gatacgtccc cggcgacgac gtgaaggcga tcgactgggg cgcgtccgcg
     3721 cggctttcct cggcacgcag ctccgacgac ttcatcctgc gcgaacgctt cgcggaggag
     3781 gcgccgcgcg tcgtcgtcat ctgcgaccgt cggcccgaga tgcagctctt cccgcgcggc
     3841 ttcccctggc tgcggaaggt cgatgcgcaa cgcgctgcga tcgagctgat cagggcgagc
     3901 gcgatcgagg cgcacggttt cctcggctac ctcgacttcg gcgagagcgg tgacgagccg
     3961 ttctggctgc caccgcagtc gcagtcgctg tgggagtacc aggatcgcga agccgaaggc
     4021 gcaccgtttc gagcgcccga gtccaacgtg gagcacgcgt tcgacttcct gatgtcgcac
     4081 ccgcgctcgg ttcccgccgg cacgttcgtg ttcgtgctct ccgactttct cgtcccgccg
     4141 cccgcggaaa cgtggctgcg cgcactcgag caacgctggg acgtgattcc cgtcgtgctg
     4201 caggacccgc tgtgggagcg cagctttccc gacctcgacg gcttcgtcgt cgagctcgca
     4261 gacgtcggcg gccattcgca gcacgtgcgc ctgcgacggc gcgaggtcgc cgagcggcgg
     4321 cgggcgaacg aaacccgctg ggaaaagctc gtcgacgatt tcgcgcgcgc atccctcgag
     4381 ccggtcgtga tcgactcgag cgaggagagc gagattctgc gcgcgttcct cgactgggca
     4441 gagacgagac aggaaggcgg cggcggagca tggtgaagct cgcggcagtc ctcggtgtca
     4501 ccgctgcgtc gctggctttc gcggca
//
LOCUS       DASVEU010000228         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1131461,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000228 DASVEU010000000
VERSION     DASVEU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131461"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VF225_09880"
     CDS             complement(<1..1073)
                     /locus_tag="VF225_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7145601.1"
                     /translation="MDDPHAVGIRLKRARDDAGLSQRQLSFSGCSPAYISRIEAGDRI
                     PSLQLLRELGKRLGVSEDYLATGVEARGQTTSLEAELALRFDDLELAEGLYEELLAEA
                     TAPDARTEALTGLGRVALRRGRSDEAVRLLEEALSTGSKDECEPPELGESLGRAYAER
                     GDLSRALAIFERCVAHFEQKGDTVLSIRFACLLGATLLETGNASRAEAVVTNALGTGE
                     PLSDPSARALLYFSQCRARSAEGDVAGASRYGRLALATLESADDARFTVQGRQILARA
                     ALASNRPEIALTHYEAARPLLESSGSRLEQARFGAGEAHALAGLDRKEDATALAARIP
                     ALLDGVSPAEAGSVYSLLAEVHA"
     gene            1228..1911
                     /locus_tag="VF225_09885"
     CDS             1228..1911
                     /locus_tag="VF225_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145602.1"
                     /translation="MEPTLYRVRMDWDAYATTQVSRYEDGEARLSLEPDARQKQLVRM
                     AMAAGGVGLARLMEGRPGDAAAWLTRSAERYRESFVGAPPESWGRPVGAVKARLLAGD
                     GEGAAADASWALAAGAAVSASPIGRYAGALAHLVLGADQEAAVTAGSLQREAPDAFPA
                     PVADALAALAARDSPAYDVAVRAVLRSFEEREAYLEDAAVADTVLVLQALAKPRVLAV
                     TLSSLLLPP"
     gene            complement(1938..2549)
                     /locus_tag="VF225_09890"
     CDS             complement(1938..2549)
                     /locus_tag="VF225_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7145603.1"
                     /translation="MAFSREAALVSALRLSYFTIAWNGVVGAAALVVSVLDGSLALAG
                     FALNALLDSSASGVLVWRFRREQRDPAAAERLERKAQSWIVVAMLTVGLYVGVQAVRA
                     LIDGSHPEGSAFGVGLATLSLIVLPWLGRMKLRVGSRLVSHALRGDGVLTLAAAVLAA
                     VTLASILVNTTLGWWWADPLAALVIAFALAAEASRLAVRHRLD"
BASE COUNT          446 a          892 c          893 g          400 t
ORIGIN      
        1 tccgcatgga cctcggccag gagcgagtag accgatccgg cctcggcggg tgacacgccg
       61 tcgagaagcg cgggaatgcg ggcagccaag gcggtggcgt cctccttgcg gtcgagcccg
      121 gcgagggcgt gcgcctcccc ggccccgaaa cgggcctgct cgagtcgcga accgcttgac
      181 tcgaggagcg gtcgggcggc ctcgtagtga gtgagcgcga tctccggccg attgctcgcg
      241 agggccgcac gggccaggat ctggcgcccc tgcaccgtga aacgggcgtc gtcggccgac
      301 tcgagcgtcg cgagcgccaa tctgccgtag cggcttgctc ctgcgacgtc gccctcggcg
      361 ctccgggccc ggcactggga gaagtagagg agcgcacgcg cggaggggtc ggacagcggc
      421 tcccccgttc ccagggcgtt cgtgacgacc gcctcggctc ggctcgcgtt tccggtctcg
      481 aggagcgttg ccccgagcag gcaggcgaag cggatgctga ggacggtgtc gcccttctgt
      541 tcgaaatgcg cgacgcagcg ctcgaagatc gcaagcgcgc gcgagagatc cccgcgctcg
      601 gcataagccc gtccgaggct ctcacccagc tccggcggct cacattcgtc cttcgagccg
      661 gtcgagagcg cctcctccag gaggcggacc gcttcgtccg accggccgcg gcggagcgcg
      721 acccggccga gccctgtgag ggcctccgtc cgggcgtctg gcgccgtggc ctcagccagc
      781 agttcctcgt agaggccttc ggcaagctcg agatcgtcga accgaagcgc aagctcggcc
      841 tcgagggacg tggtctggcc gcgagcttcg acgcccgtcg ccaggtaatc ctcgctcacc
      901 ccaagccgct tgccgagctc ccggagcagc tgcagcgagg gaatccggtc gcctgcctcg
      961 atcctcgaga tgtaggcggg cgagcagccc gagaaggaga gctgccgctg gctcaggcca
     1021 gcgtcgtcgc gcgctctctt gagcctgatt ccgacggcat gagggtcgtc cacgtgtgtc
     1081 gacttttgtc taggcaaccg ttctccaatg tgtgccagag cgtgggttca tcgatgcgtc
     1141 gcgccacaat actctgtatg tcggccacgg tcgccactac cggttgaaaa caaatgtata
     1201 cacgtagtgt cagcatactc tcgactcgtg gagccaaccc tgtaccgtgt ccgaatggac
     1261 tgggacgcct acgcgactac ccaggtgtcg cggtacgagg acggcgaggc gcgactctcc
     1321 ctggaaccgg atgcgcgaca gaagcagctg gttcggatgg ctatggccgc gggcggagtc
     1381 gggctcgcaa ggctgatgga gggtcgcccc ggggacgcag cggcatggct gacccgctcg
     1441 gctgagcgct accgcgagag cttcgtagga gctccgcccg agagctgggg gaggcccgtg
     1501 ggcgccgtca aggcccggct gctcgccggg gacggagaag gggcggccgc cgacgcgagt
     1561 tgggcgctcg cggcgggggc cgcagtcagc gcttcgccga tcgggcgcta cgcgggagca
     1621 ttggcccatc tcgtcctcgg ggccgaccaa gaagccgccg tcacggccgg gtcgctccag
     1681 cgtgaggcac ccgatgcgtt cccggcgcct gtggcagacg cgctcgcagc gctcgccgcc
     1741 cgcgactcgc cggcgtacga cgtagccgta cgggccgtcc tccgctcgtt cgaggagcgg
     1801 gaggcctacc tcgaggacgc agccgtcgcc gacaccgtgc tcgttctgca ggcgctcgcg
     1861 aagcctcgcg tccttgcggt cacgctttcc tcactgctct tgcctcccta ggagggtttg
     1921 acgaaataaa ggactgacta gtctaagcgg tgtcgtacag ccaaacgcga tgcctccgcc
     1981 gctagcgcga acgcgataac caaggcggcg agcgggtcgg cccaccacca cccgagcgtc
     2041 gtgttgacga ggatggacgc gagcgtgacc gcagcgagca cggcagctgc cagcgttagc
     2101 acgccgtcgc cgcgaagcgc atggctcacc agacgcgaac ccacacgtag cttcatccgt
     2161 ccgagccacg gcaagacgat gagtgagagt gttgcaaggc caaccccaaa cgccgatcct
     2221 tcgggatgcg agccgtcgat aagcgcgcgg actgcctgga cgccgacgta caggcccacg
     2281 gtcagcatcg ccacgacaat ccaggactga gccttgcgct ccaatcgttc tgctgccgct
     2341 ggatcgcgct gctctctcct gaacctccaa accaggacgc ctgacgccga ggagtcgagg
     2401 agcgcgttca gcgcgaaccc ggccaaggcg aggcttccat ccaacacgga aacgactagg
     2461 gcagcggctc caacgacacc gttccaggca atcgtaaagt aggagagtcg aagcgcagag
     2521 acgagcgccg cctcgcgtga gaacgccact cagcgactct atcgacggcc catcttgagg
     2581 gtccgcaacc tgaaattcgg tccggcttat gccgtcggac cctctcaata g
//
LOCUS       DASVEU010000229         6167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_937785,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000229 DASVEU010000000
VERSION     DASVEU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937785"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..629)
                     /locus_tag="VF225_09895"
     CDS             complement(9..629)
                     /locus_tag="VF225_09895"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7145604.1"
                     /translation="MSDRLTVGVAARLSRYLQVLTQAKKMGKERISSQEISDYTNINA
                     TQIRRDLSAFGKFGKRGVGYNIDSLLGEIRKILRTQGQHNIALIGAGRLGQAIASSPI
                     FAEHGINIAAVFDTDPDKVGRPVGNVPVSDYARLSNTIREKNIIVGVVAVPADNAQRV
                     ADDLVESGVRIIFNYSEALLDVPQDVAVHTSNPAVELLYALYFYLT"
     gene            complement(708..968)
                     /locus_tag="VF225_09900"
     CDS             complement(708..968)
                     /locus_tag="VF225_09900"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7145605.1"
                     /translation="MMPTQSEPKVVLYHGKGCHLCERARWIVAEIRQEMRFELEEIEI
                     DGDPALEARYREWLPVVEIDGERAFVYYVDPDAFRRKVAAQS"
     gene            complement(1107..2381)
                     /locus_tag="VF225_09905"
     CDS             complement(1107..2381)
                     /locus_tag="VF225_09905"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7145606.1"
                     /translation="MTTVDAPVSHLRENPFEIARSQLRKVADTFAIDPNLVNVLQECK
                     KALSVSVPVSMDDGTTRVFQGYRVTHNVARGPSKGGIRYHPDVTLDEVKALAMWMTWK
                     CALAGIPFGGAKGGVVCNPKTMSEGELQRMTRRFTSEIINEIGPEKDIPAPDVGTDGR
                     VMAWIFDTYSMNKGHSVLGVVTGKPLTIGGSLGREEATARGALYCVREAVRKQQLSLQ
                     GTTVAVQGFGNVGSYLAKFLAEDGATVIAVSDSSSGLHNPNGIDVQAAIAHKRETGSL
                     AGFRGAEPISNEDLLLLECDVLAPCALEQVITAENAHRVRAKIIVEGANGPVTPVADE
                     ILEEKGVLVLPDILANAGGVIVSYFEWVQGLQEYFWKEAEVNAKLNDIITRAFNETWA
                     LMESRSVSMRLAAYGLAVQRVAEATVTRGIYP"
     gene            complement(2441..5017)
                     /locus_tag="VF225_09910"
     CDS             complement(2441..5017)
                     /locus_tag="VF225_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7145607.1"
                     /translation="MPTRPPREPFNALVLAALAVVATAVLIAAGGGSRGIVQQGEPSW
                     QGLAGAQRPRVAVGQRVIVLLKAPALADRVGRVGGLATSEEERRWMETALSSQKLLVA
                     RLRVQGVVVQPEFSYTRTVNGFSAAFDARGIALLERAPEVAGVYPVRAAYPASFSSRL
                     LTASEFGERPNIGLSDKDGRGVTIALLDTGVDRSQPFLRGRVSGGIDIVGGEAGAVAA
                     TRPDDPSQLEQHGTEMAGLLVGSGGPGALAGVAPGATVLPIRIAGWQRDASANWAVYG
                     RTDQLLAGLERAVDPNGDGDAHDAARVALVGVAEPFAAFADGPLARAAAGATRLDTLV
                     VAPAGNDGPAGPSYGSVAGPGGSPAVLTVGAVDLRPRFGQVLVVLRTGLRIEFDGMRP
                     LAGAVTPIRALDVGIGAPRSQTTGAVGYRGAVPLLDFFDRRGSSLVAGRAALLPAGVD
                     PSGSVANAAQAGAVAVLFYGADLPAGGIGLDERAAIPAVSVPEDVAQTLLDRIRSGVQ
                     TTVSLGAVRTESNAGEGRVTRFSSTGLAFDGRVKPEVVAPGVELETAEPGANADGSAR
                     YGTVNGTSAAAALVAGSAALLAQARPDLDADALKSLLVGAARPLPDDRVTAQGVGLID
                     LGGATATEVGASPASLALGRADRRNWRTEQEFVLRNVSFRPLILSLDVRVAQEGAAAL
                     RFDIAPQHLSLGPGRSIRVGVRARVTSKLVGAAPAEGVVIVTPAAGREIRVPWTITFG
                     PRLPAGLASVRLSMRAFAPSDAAPALLSFVAGSVRRSAGEPDVLPLSRLDLELWSPDG
                     GRIGLLARLRDVLPGRYSFGVTGRDPAGAVLPKGDYQLRLVGFATDSGPPTIRTVGFT
                     IK"
     gene            complement(4995..5963)
                     /gene="tsaD"
                     /locus_tag="VF225_09915"
     CDS             complement(4995..5963)
                     /gene="tsaD"
                     /locus_tag="VF225_09915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7145608.1"
                     /translation="MILGLETSCDETAAAVITSEGRILSNIVSSQADLHARYGGVVPE
                     VASRRHLELVTPVVQEAMAEAETSLDEVDLIGVTAGPGLIGALLVGVAAAKAIAWARR
                     LPLAAVDHLQGHVASLYLQPDPVEPPFLCLLASGGHTLLLDVQDHTSKPRVLGTTLDD
                     AAGEAFDKGARLLGLGYPGGAEIDRIAREGDPSAYEFPVARVSGLDFSFSGLKTALLY
                     AVRELTAEELERGRADLAASYQRAIVRALVERTRQAAEQIGATRIAVVGGVAANFELR
                     AALPQATAAPPALCTDNAAMIASAARFSSPIPYPGYLGLDAYASTA"
     gene            complement(5960..>6167)
                     /locus_tag="VF225_09920"
     CDS             complement(5960..>6167)
                     /locus_tag="VF225_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145609.1"
                     /translation="ASLLERLFELTAGDGRRGFTLEVRVSNEVAIRLYEQAGFKARGV
                     RRGYYTDNREDALIMWRDPEPRPA"
BASE COUNT         1023 a         2090 c         2073 g          981 t
ORIGIN      
        1 ccttatggtt acgtcaagta gaagtagagc gcgtagagca gctcgaccgc cgggttcgac
       61 gtgtgaacgg cgacgtcctg cggcacgtcg agcagggctt ccgagtagtt gaagatgatc
      121 ctgacgcctg actcgacgag gtcgtccgcg acgcgctgcg cgttgtcggc gggaactgcg
      181 acaacgccga cgatgatgtt cttctcccga atcgtgttcg agaggcgcgc atagtcgctg
      241 acagggacgt tcccgaccgg acggcccacc ttgtccggat cggtgtcgaa cactgcagcg
      301 atgttgattc cgtgctccgc gaagatcggc gaactggcaa tcgcctgacc gaggcgcccg
      361 gccccgatca gcgcgatgtt gtgctggccc tgggtgcgga ggatcttgcg gatctctccg
      421 agcagcgagt cgatgttgta gccgaccccg cgcttcccga acttcccgaa cgcggagaga
      481 tcacgccgga tctgggtcgc gttgatgttc gtgtagtcgg agatctcctg ggaggagatg
      541 cgctcctttc ccatcttctt cgcctgcgtc agcacctgga ggtagcggga caggcgcgcc
      601 gccactccga ccgtcaaacg atcagacaca ggcacagtgc tcctggattc gagagtttgt
      661 gacaacccga acccgttgta tcacgcgacg ccggggccgc gcgtaggtca actttgtgcg
      721 gccactttgc gacgaaaggc atctgggtcg acgtagtaga cgaaggcccg ctcgccgtcg
      781 atctccacaa cgggaagcca ctccctgtag cgcgcctcca gcgctggatc gccgtcgatc
      841 tcgatctcct cgagctcgaa cctcatctct tgccgaatct ccgcgacgat ccaccgcgcc
      901 cgctcacaca ggtggcaacc ctttccgtgg tagaggacga ctttcggttc gctttgcgtc
      961 ggcatcatcc gcggaggcta aacatcgctc tggcttgagc gcgttgcgga gcgaaggcgt
     1021 gaccgcgtca cgccgtagcg cagagaacag gcggcatgcg ggtcgaggag ggggcacacg
     1081 ggggaaccat gggttccccc gtgtgattag ggatagatgc cgcgcgtgac cgtagcttca
     1141 gcgacgcgct ggacggccaa gccgtaggca gcgaggcgca tcgagacact tcggctctcc
     1201 atcagggccc aggtctcgtt gaacgcgcgc gtgatgatgt cgttcagctt cgcgttgacc
     1261 tcggcttcct tccagaagta ctcctgcagt ccctggaccc attcgaagta ggagacgatc
     1321 acgccgcctg cgttcgccag gatgtcgggg aggacgagta cgcccttctc ctcgaggatc
     1381 tcgtcggcaa cgggtgtgac cggaccgttg gcgccttcga cgatgatctt tgctctgacc
     1441 ctgtgggcgt tctccgcggt gatcacctgc tccagggcgc acggcgcaag aacgtcgcac
     1501 tcgagcagga gcaggtcttc gttcgagatc ggctcggcac ctctgaagcc ggcgagcgag
     1561 cccgtctcac gtttgtgtgc gatcgccgcc tgaacgtcga tgccgttcgg gttgtgaagg
     1621 ccggagctcg aatccgagac ggcaatcacc gtcgccccgt cctccgcgag aaatttcgcg
     1681 agataggagc cgacgttgcc gaagccttgc accgcgaccg tcgtgccctg cagcgacagc
     1741 tgctgcttac gcacggcttc ccgcacgcaa tagagggcgc cgcgcgccgt cgcctcctca
     1801 cggccgagcg agccgccgat cgtgagcggt ttgccggtga cgactccgag gaccgagtgg
     1861 cccttgttca tcgagtaggt gtcgaagatc caggccatca cgcgcccgtc ggtgccgacg
     1921 tccggcgcgg ggatgtcttt ctccggtccg atctcgttga tgatctctga ggtgaagcgg
     1981 cgcgtcatgc gctgcagctc gccctccgac atcgtcttgg gattgcagac gacgccgccc
     2041 ttggcgccgc cgaacgggat tcccgccaga gcgcacttcc acgtcatcca cattgcgagt
     2101 gctttgacct cgtcgagcgt gacgtccggg tggtagcgga taccgccctt cgaagggccg
     2161 cgggcgacgt tgtgcgtgac gcggtacccc tggaacacgc gcgtcgttcc gtcgtccatc
     2221 gagacgggta ccgaaacaga gagcgccttc ttgcactcct gcaggacatt gacgaggttc
     2281 gggtcgatcg cgaacgtgtc tgcgaccttc ctgagctggg acctcgcgat ctcgaagggg
     2341 ttctcgcgaa ggtgcgagac aggcgcgtcg acggtggtca agtcagctcc tttttccgcg
     2401 caaatgggaa cattttcggc tttggctggg ccggcggagc ctacttgatc gtgaaaccga
     2461 ccgttcggat agtcggcggc cctgagtcgg tcgcgaagcc caccagcctg agctggtaat
     2521 cccccttggg gaggacggcc ccggccgggt cgcgaccggt caccccgaac gaatagcgcc
     2581 ccggcagcac atcgcgtagg cgcgcgagca aaccgatgcg gccgccgtcc gggctccata
     2641 gttcgaggtc gaggcgcgac agcggaagca cgtccggctc accggccgaa cgccgcaccg
     2701 agcctgcgac gaaactcagc aacgcaggcg cagcatcaga gggcgcgaac gcgcgcatcg
     2761 agagccgaac ggaggcgagg cccgccggca gacgcggccc gaaggtaatc gtccacggca
     2821 cgcgaatctc cctgccggct gccggcgtga cgattacgac gccttccgcg ggtgcggctc
     2881 ccacgagctt gctcgtcact cgtgcgcgaa cgccgacccg aatcgatctt ccggggccga
     2941 gcgatagatg ctgcggtgcg atgtcgaacc tgagcgccgc cgctccctcc tgggcgacgc
     3001 gcacatcgag cgacaggatc agcgggcgga aggagacatt gcggagaacg aactcctgct
     3061 ccgtgcgcca gttgcggcgg tcggctcgtc cgagggcgag tgacgctggg ctcgcgccaa
     3121 cctcggtcgc ggtcgcaccg ccgagatcga tcagcccgac gccctgggcg gtgacgcggt
     3181 cgtcgggaag cggacgcgcg gcgccgacga gcaggctctt caaggcatcg gcgtccaggt
     3241 ctgggcgcgc ctgtgcaaga agtgccgcgg agccggcgac gagggcagcc gccgcactcg
     3301 tgccgttgac cgtgccatag cgcgccgaac cgtcggcgtt cgctcccggc tcggcggtct
     3361 cgagttcgac gcccggcgcc accacctcag gcttgacccg gccgtcgaac gcgagaccgg
     3421 tcgaggagaa gcgcgtcacc ctcccctcgc ccgcgttcga ctcggtccga acagcgccga
     3481 gcgacacggt cgtctgcact ccgctgcgaa tcctgtccag cagcgtctgc gcgacgtcct
     3541 cgggaacgga gaccgcaggt atcgctgctc tctcgtcgag gccgatgccg cctgccggaa
     3601 gatctgcgcc gtagaacagg acggctaccg cccctgcctg ggcggcgttc gcgaccgatc
     3661 cgctgggatc gactccggca gggaggagcg cggcgcggcc ggcgaccagg ctggagcccc
     3721 ggcggtcgaa gaagtcgagt agcggaacgg cgccgcgata gccgaccgct ccggtcgtct
     3781 gcgatctcgg cgcgccgatc ccgacgtcga gtgcgcggat aggtgtgacc gcgccggcga
     3841 gcggcctcat cccgtcgaac tcgatgcgaa gtcctgtgcg caggaccacg agcacctgtc
     3901 cgaagcgggg acgcaggtcg acagcgccga ccgtcaggac cgcgggcgac cctccggggc
     3961 ctgccacact gccgtagctt ggccccgcgg ggccgtcgtt cccggcgggg gccacgacca
     4021 gcgtgtcgag ccgcgtggca ccggctgcgg cccgtgcaag cggcccgtcg gcaaacgcag
     4081 cgaacggctc cgcgacaccg accaatgcga cccgcgctgc atcatgggca tcgccgtcgc
     4141 cgttcgggtc gaccgctcgt tccaacccgg cgagtagctg gtcagtgcgt ccgtaaacag
     4201 cccagttcgc ggacgcgtct cgttgccacc cggcgatgcg gatcggtagc actgtcgcgc
     4261 ccggtgcgac gcccgcgagc gcacctggcc cgcccgaacc gaccagcagg ccggccatct
     4321 ccgtaccgtg ctgttcgagc tgggaggggt cgtcggggcg tgtagcggcc accgcaccgg
     4381 cctcgccgcc gacgatgtcg atgccgcccg agacccggcc gcgcaggaag ggctgtgacc
     4441 gatccacacc cgtgtcgagc agggcgatcg tcacgccgcg accgtccttg tcggagaggc
     4501 cgatgttcgg gcgttcgccg aactcggagg ccgtcagcag acgcgagctg aacgaagcgg
     4561 gataggcggc gcgaacggga tagacgcccg ccacctcggg ggcacgctcg agtagcgcga
     4621 ttccgcgcgc gtcgaacgcc gccgagaagc cgttgacggt ccgtgtgtaa ctgaactcgg
     4681 gttggacgac gacgccctgg acgcgcaggc gcgctacgag cagtttctgc gacgagagag
     4741 cggtctccat ccagcgacgt tcctcttcgc tcgtggccag gcccccgacg cgaccgacgc
     4801 ggtccgccag cgccggcgcc ttgagcagga cgatgactcg ctggccgact gccacgcgcg
     4861 gccgttgcgc gcccgcgagc ccctgccagc tcggctctcc ctgctggaca atgcccctgc
     4921 tcccccctcc ggcagcgatg aggaccgccg tggcgacgac cgcgagagcc gcaagcacca
     4981 gggcattgaa cggctcacgc ggtggacgcg taggcatcga gcccaagata gccgggatag
     5041 gggatgggac tggagaaccg ggcggccgag gcaatcatcg ccgcgttgtc ggtacagagc
     5101 gcgggcggcg cagccgtggc ctgtggaagc gcggccctca gctcgaagtt ggcagcgact
     5161 ccgccgacga ccgcgatcct ggtcgctccg atctgctcgg cagcctggcg tgtgcgctcg
     5221 acgagcgcac gcacgatcgc gcgctgatag ctcgcagcaa gatcagctcg gccacgctct
     5281 agctcttccg ccgtcagttc cctgaccgcg tagagcaagg cggtcttcag accggagaac
     5341 gagaagtcga gcccggagac acgcgcaacg ggaaactcgt acgcgctcgg gtcgccctcg
     5401 cgagcaatgc ggtcgatctc cgcgccaccg ggatagccga ggccgagaag ccgcgctccc
     5461 ttgtcgaagg cctcccccgc cgcatcgtcc agcgtcgttc cgaggacccg cggtttgctc
     5521 gtgtgatcct gaacgtcgag cagcaacgtg tggccgccgc tcgccagcag gcagaggaac
     5581 ggcggctcga ccggatccgg ctggagatag agggaggcga cgtggccttg cagatggtcg
     5641 acggcggcca gcggcagccg ccgcgcccag gcgatcgcct tggcagccgc aacaccgacg
     5701 agcagcgcgc cgatcagccc ggggccggcg gtcactccga tgaggtcgac ttcgtcgagc
     5761 gaggtctccg cctcggccat cgcctcctgc acgacgggcg tgacgagctc gaggtgacgc
     5821 cgcgatgcaa cttcgggaac gaccccgccg tagcgcgcat gcagatcagc ttgcgacgag
     5881 acgatgttcg aaaggatccg gccctccgac gtgatcaccg ccgcagcggt ctcgtcgcag
     5941 gaagtctcga gccccagtat caagcggggc gcggctcagg gtctcgccac atgatcaacg
     6001 catcctcgcg gttgtccgtg tagtagccgc ggcgcactcc gcgcgctttg aacccggctt
     6061 gctcgtacag cctgatcgcg acctcgttcg aaactcgtac ctcgagcgtg aagcctcggc
     6121 gtccgtctcc tgcggtgagc tcgaacaacc gctcgagcag gctcgcc
//
LOCUS       DASVEU010000230        21313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1326946,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000230 DASVEU010000000
VERSION     DASVEU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 21313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21313
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326946"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF225_09925"
     CDS             complement(<1..322)
                     /locus_tag="VF225_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7145610.1"
                     /translation="MVKRFGSTTALAGVDLEVAEGMVFGLLGPNGAGKTTLIRILATL
                     LEPDAGRAELLGHDVVSESASVRELLALTGQFAAVDELLTGRENLEMFGRLFRLSRAE
                     ARRRA"
     gene            1167..1403
                     /locus_tag="VF225_09930"
     CDS             1167..1403
                     /locus_tag="VF225_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145611.1"
                     /translation="MSVTDEPISFEQQIKPLFRERDRQSMTWAFDLWSRDDVAANSEA
                     ILDKLRDGTMPCDGAWSDEQIRVFQRWVEAGSPA"
     gene            complement(1400..2356)
                     /locus_tag="VF225_09935"
     CDS             complement(1400..2356)
                     /locus_tag="VF225_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7145612.1"
                     /translation="MEICAVLGAGGGIGNAVVWELAGRGLRVRAVSRDGRARVPDGVE
                     ACAADLNTAEGAARACADAAVVYHCVQPRYTRWAEEFAALNATIVAAVTEAGPKLVVA
                     DNLYMYGPISGPISETSQQEPQSKKGRLRKQLAASFLAAAENHGLRVTIGRASDYFGP
                     GGAHSVAGMLVTGALAGKTVRWPANADFPHTFSYLPDVARGLAVLGERDEANGTAWVL
                     PAAPPITARAFVALIGQACGRTVKLSVTSKLAMRAAGLGIPEAREVPDIWYQFAAPFV
                     VDGSAFDRAFGPLQITPLAQAVHDTVAWYQATAEGAQPPRGG"
     gene            2445..3872
                     /locus_tag="VF225_09940"
     CDS             2445..3872
                     /locus_tag="VF225_09940"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX7145613.1"
                     /translation="MARAEHAQKAAPSAFIGPPESRARGKAARGEAPRSSHSLLPLAD
                     DRDALALLESQAQLRVPELIPIRYGRMVASPLAFFRGAATVMAHDLASTPTAGLRVQL
                     SGDAHFLNFGGYASPERDLVFDLNDFDETFPGPFEWDVKRLATSFEIAGRDRSFARTE
                     RKDAVLEAARSYREAMRRFAAMGELDVWYARLDAKAIDQQLMEAHDKKLGRTLRRAEA
                     KGHRHDRMLALARLTKTVDGERRFISDPPLIVPVAELDEREDEAGLEPLLHALFRRYR
                     RTLPAERQTLIERFRYADLARKVVGIGSVGTRSWVMLLLGRDDGDPLFLQIKEALPSV
                     LEPLLGRSPFATHGQRVVVGQRLMQAASDIFLGWVHSDEELDGRAHDFYVRQLWDWKT
                     SVDVETILPRGLTASATACGWTLARAHARSGDRIAIAAYLGASDRFDRAVAEFAAGYA
                     DLNERDHRHLLEAVAEGRVDAAEGV"
     gene            complement(3916..4224)
                     /locus_tag="VF225_09945"
     CDS             complement(3916..4224)
                     /locus_tag="VF225_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145614.1"
                     /translation="MAVGILTAAPEFTKQVYDDVTEKMFGHPAPMREDEAPEGLIVHS
                     AGQGEQGYYVYDVWESREAFDRFMEEKLGPALGEVMGGPPPEGGAPQYFPIDVLIIPH
                     "
     gene            complement(4288..5034)
                     /locus_tag="VF225_09950"
     CDS             complement(4288..5034)
                     /locus_tag="VF225_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145615.1"
                     /translation="MNRALVLPMVGVALIAALVLGAGPSTAGKPKPPSWALHGSYSAK
                     IDPANFVAAVDNRYFPLKPGTTFHYKGFKGKTPQIDDMVVTRQTIRILGVTCTVVRDT
                     VSQHGKPLERTFDWYAQDKQGNVWYMGEDARELQNGRFVRANDSWKAGVNGAKPGIIM
                     RGNPRPNEVYRQEYYPPGGALDQGRVLGLAVSVKVPYGTFTRSLATIEWSPVEPQFEK
                     KYYVSGIGEVKEQVTQGGHERFELVSLTHG"
     gene            5188..6744
                     /locus_tag="VF225_09955"
     CDS             5188..6744
                     /locus_tag="VF225_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7145616.1"
                     /translation="MAAPAIPSLSASQLTTLAAAGEERRADVGDVLFTVGDATYPFIA
                     IREGEVAIRDAAGNEIIRHGASSFLGELNLLSGQTVFLTAVVTKPLRYVAVDRDALRA
                     LLFEDGPLSDLVLSTFIARREALQRVQGIGLEIVGPRSSEATMQMLDFARANRLPFTW
                     HEIAPPDGIEPPLVRLPEGTELQHASRGEVLRALGIGRELAPREEVDLLVVGGGPAGL
                     AAAVYGASEGLDTLIVEGTALGGQAGSSRRIENYLGFPAGISGTELTSRAVSQARKFG
                     ARPATPYRAIALEPGDGRHLVRLEEEHEIAARAVVLATGAAYRRLPLDGLSDYEGVSV
                     FYAAGPPEAQQCGASRVAVVGGGNSAGQAAVWLARGGALVTLLHRRADLSETMSDYLI
                     RELERYGVAVRDGSEIAALHGTEGQLAEVTLKNGERLPFAACFFFLGAQPCTEWLDDA
                     VARAADGFILTGNAAGADNLLETSVPGIFAAGDVRSGSSKRCAAAVGEGSMAVQFVHA
                     HLAALASRVG"
     gene            6741..7058
                     /locus_tag="VF225_09960"
     CDS             6741..7058
                     /locus_tag="VF225_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX7145617.1"
                     /translation="MSELCTHLDQVELSELPASVDGCEECLRDGGKWLHLRICLTCGH
                     VGCCDDSPNRHATAHHHATSHPIIRSLEPGEDWSWCYLDEVAFVLDGVRGTTRIPPSP
                     LLS"
     gene            complement(7072..9813)
                     /locus_tag="VF225_09965"
     CDS             complement(7072..9813)
                     /locus_tag="VF225_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7145618.1"
                     /translation="MATRASGVQLLGRQREREVLDRVLEAARDGQGGVLAVYGEPGVG
                     KTALLDYAIEVGADFRVVRTVGVEGEMELAFAALQQLCSPSLDLMDRLPDPQRDAFEV
                     AFGLSAGRPPNPFLVGLAVLNLLSEAAEERPLLCLVDDAQWLDRASARVLAFVARRLL
                     AEKIAMVFAAREPIDALAGLAEFHVEPLGRRDARALLDSVLPARLDERVLERIVVETR
                     GNPLALLELPRGLTPAQLAGGFGLPAALPLSAQIEESFARRLARLPRDARRLLLVAAA
                     DPTGDAALEWRAAQRLGIAESASHTAESDGLLAFDAGVEFRHPLVRSAVYRAAGADER
                     SEVHRALAEATDPTIDPDRRAWHRAQAASRPDEDVAAELERSAARAQARGGFAAAAVF
                     LERSSVLTLDRAQRAARALAAAEAKEQAGALDDALALVSSAEAGPLDDVQRAQVDVIR
                     ARISFAADRGSEAPPLLLTAAKRLEPLDGAAARQIYLDALTAALFAGRLGGEVDTRQV
                     AAAARAAPPPEPPAHGADLLLDGLAVLITDGAAAGTPVLRKALTAFGNDEIGTDEGLR
                     WLWLAGRAAGYIWDYENWDALTRAQARIARESGALTVLPLTLSTRAGVELYAGNLADA
                     NLLIAEANAITDATDSRDVPYAPLALVAFRGREPDASRLIQATTEDFLARGEGMGLTL
                     AQWATACLHNGLARYDIALAAAEQAAEDPHELWFSPWTMVELIEAATRSGQNDRAASA
                     LEVLSETTKASGTPWAQGVEARSRALLADGEAAETLYREAIQHLRPTRLRVDLARTHL
                     LYGEWLRRERRRIDARAELRVAHELFSEFGMEAFAERARVELEATGERARKRTLDTLG
                     DLTPQEAQISRLVAQGHTNREIAAQLFISPSTVEYHLRKVFRKLGVKSRTQLARRVA"
     gene            9922..10581
                     /locus_tag="VF225_09970"
     CDS             9922..10581
                     /locus_tag="VF225_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7145619.1"
                     /translation="MTTAIIGVGNIGGALARHLVGGGESVVLAAKDKSRSEALAQELG
                     PLARAASVEDAIADADVVVFAVWLDTIKELMARDAGLLDGKVVVDPSNPLGFDKSGQM
                     IRTLPEEQSSGSVVAALLPAGAHYVKAFGTLAADTLASGANREPRRAVLFYATDDDAA
                     ATTVERLIRAAGFDPLKAGGVADAGRIEMPGGDLHQAGLNGAILDLDEAGAAVAGEVP
                     T"
     gene            10578..11537
                     /locus_tag="VF225_09975"
     CDS             10578..11537
                     /locus_tag="VF225_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7145620.1"
                     /translation="MSATETPALPDYAPVPRSALGPALNDQGYYVGRVERNLYWITDG
                     TYQSAFLTTSDGVVLLDAPPTIGNNIQRAVDEIAAANGVSNKVTHLIYSHHHADHAGA
                     SSLFDKNVTRIGHEETRQLLLRDDDPARPPNEETFQDRRTLEIGGERIDLAWHGPNHS
                     PDNILIHLPDHDTLMLIDIVNPGWAPIYLSNLTEDIPGYVEAPANALAYSWKHFIGGH
                     NGRLGTRDDVTLHQQYMADIAESSRKAIDTVDPTPYFQKYGENVWAALKGYLDEVTAT
                     AAAPVIEKYTGVIAAADVFTESTTFWVMQSIRLDLGYGSQVHP"
     gene            11537..12151
                     /locus_tag="VF225_09980"
     CDS             11537..12151
                     /locus_tag="VF225_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX7145621.1"
                     /translation="MASLPVRDPVGDHLLTPQNSALVVIDYQPSQFAGVRSIDRDLLL
                     ENIVSTVKTAKAFGLPIVHSTINVATGRGQPTVPELAELLEDNPPIDRTTTNAWEDAD
                     FVAAVRATGRRKLIACALWTEICMTFPALDALREGYDVYPVVDAIGGTSEEAHRAGLE
                     RVVQAGGRPISWVALAVELQRDWAREETVADVIEIVLTERLLKT"
     gene            12148..12810
                     /locus_tag="VF225_09985"
     CDS             12148..12810
                     /locus_tag="VF225_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX7145622.1"
                     /translation="MNVESPSRERAAIADPEAREFLRQADPVLARLIDARPDFRPRAW
                     MDELPPLDAFGTLVFQVAGQQLSVSSTRAIVSHLQERFGGHLPSPAELLAADPQVPRD
                     SGFSARKGETLRALAERFVDGRLSDEALSRMTDDEVEAVLTEVPGVGLWTARGFLLVA
                     LDRPDVFLSGDLALRRAIQRAYGFDHLPTEDEVAQVSDRWRPYRSLAVSYLFASEYDG
                     QP"
     gene            12807..13382
                     /locus_tag="VF225_09990"
     CDS             12807..13382
                     /locus_tag="VF225_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7145623.1"
                     /translation="MTGSVGIIGAGRLGQAMARTALRAGRTVVIANSRGPESLAAVAS
                     EVGATAGTVGEAATAGIVVIAVPWDRVPAAVRGLEWNGQIVIDATNDWAADDLNGRTS
                     SELVAELVAGARVAKAANTLGAEVLASDPHEAGGQRVIFVSGDDADAKAEVVALFEDA
                     GFASIDLGNLATGGAMQQIHHPLAGVNLIRL"
     gene            13396..13863
                     /locus_tag="VF225_09995"
     CDS             13396..13863
                     /locus_tag="VF225_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7145624.1"
                     /translation="MAVHTELSPTEAAGRLALRELFDAYAHCADRRDAEGQKALFTDD
                     TRFVVYMDGEGSEPTYVLQGREALTPVFEDLNRYEVTTHFNGQSTVTIEGNRATGESY
                     TIAHHIYTEDGDRKIMIASLRYLDTFTKIDQSWYFAERKLILDWSETRSLLGS"
     gene            complement(13896..15143)
                     /locus_tag="VF225_10000"
     CDS             complement(13896..15143)
                     /locus_tag="VF225_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX7145625.1"
                     /translation="MTTLAEAHPAWDRRRLVYGDWTWLVRDGLDVLRLAFIAGTIVFA
                     VQGRSTAVALTAASAVLLIARVIDLPRWFDFGLIVAMSLIAWGTALSLYGSWFYYDKI
                     VHGLSPVGYVPVLYIALVRLGVVPDPGNAIRERRVARISGIFIVTLAIGMAVGAGYES
                     VEWFEDKFLGGHFVGGLWDTETDLLCDEGGSLVGASFLTVWALRGWTSRRLTVVPVPG
                     PTETPVETAADRLRPGRSQWQRRLPGLPLAVLGAVAVAAGALLLALPTPTVRTVGIIV
                     GITLVASAAVEIVELARHHDRVKRADRLIAIAALAGAGTVVLAWPTISQLALLYAVGA
                     SAVALGVAEAAALSTLPLSSRERWLGALSSIVAFVFGIAMLGRPGGSLGAVINLLGLY
                     LVVIGALRLLQAIDAWRRQRRAH"
     gene            complement(15140..16093)
                     /locus_tag="VF225_10005"
     CDS             complement(15140..16093)
                     /locus_tag="VF225_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7145626.1"
                     /translation="MLWFQVVRCCRGIATAVVLVVGLATLLGLLDRVFWVFEIADVFR
                     LQYLVLLLAAALAALMLRRSRLALVAVVLAAVNAVVMGIPVAATATAVGARPKGSLRV
                     LVTNVEVGNTDFAAVDRLVAQSRPDVLGLIELTPPMARHLHAALPAYQVRILDVRDDA
                     YGIGVYSRLPLLSAKIARFPSNGPPTVVAHVRVAGKPVAVIVTHVHTTFAGSIHVRHL
                     DALAAAARSQFGKRVIVCGDFNTPPWSGPLRDFAAKAHVRDLYGGRAWAGYSWPTWGY
                     ALRVPLDNCFVSNSVAVTGHHDGSGVGSDHRPLVVDIGVLK"
     gene            complement(16511..16933)
                     /locus_tag="VF225_10010"
     CDS             complement(16511..16933)
                     /locus_tag="VF225_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145627.1"
                     /translation="MPMIDVYAAEGTFADPHGLARDLAAAVMRWEQVPDLPLFANNTA
                     AFIHDMDAAAISNVAGESNYVRVQVLTPVGVLDRDKQLGVVKELTDIVAAAAGDTSLR
                     ERTWVLLSESPEGGWGIGGHANTNADIAAAARAALAGQ"
     gene            complement(16933..17778)
                     /locus_tag="VF225_10015"
     CDS             complement(16933..17778)
                     /locus_tag="VF225_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145628.1"
                     /translation="MSVTEVASTQLVTAGNAIEYAYRDLGEGDVPLVLLQHFRGNLDN
                     WDPALIDALAGSRRVVTFDNVGVGASTGSTPNTIEAMAHDAIAFVEAMEFQRVDLLGF
                     SIGSFVAQEIALIRPDLLRRVVLASSAPQGAEGMHGWAPEVIGAIGEPETNPQGYLEV
                     FFAPTTTSREAGQQAAGRIFGRTGDRDEPTTWQTRQAQYDAVCAWGIPNHSLLQRVAA
                     IVLPVFVANGDSDPMILPRYSHLLAGLLPDARVKIYPDSAHGFLFQHHEEFAADVHAF
                     LEETS"
     gene            complement(17803..18510)
                     /locus_tag="VF225_10020"
     CDS             complement(17803..18510)
                     /locus_tag="VF225_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145629.1"
                     /translation="MNGRPNIVLVHGAWADGSCWSGVIERLQAEGFQVRAPQFPLTSL
                     ADDVARLRQVLEFQNGPTIVVGHSYGGQIMTALGSDAPDVVGLVYIAAFGLDQGESLG
                     ALLSQGPVTPSLAHLFTDARGYGWLSEDDFVNHFAADVDPKRARVLYAVQQALASSAF
                     TDVMGEPAWKSLPTWYLVAQNDEAIPPDAERQFAARMGATTVEIPASHVAMVSHPGEV
                     VDLIEKAAESVGAAAAR"
     gene            18640..>21313
                     /locus_tag="VF225_10025"
     CDS             18640..>21313
                     /locus_tag="VF225_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7145630.1"
                     /translation="MSTVPAREFHGRDAELALIRGELERLSDGAEAVVVVEGAAGMGK
                     SRLLAEAAAIARSLGIRVGRSAADSSETMVELATLLAALFDGTEPLLDPGTLSTLHAQ
                     PEQRFWLLRDLQQLLERAALESPLLIAVDDAHWADAGTVAAIRTLPTRLMGLPIAWVI
                     ALRPPREATPLVHAVEHLKQDGAQTIVLGPLDDDAVVQLAAEILAAQPDQSILKQLAE
                     ADGSPFLVVETLLGLQEEERIRVVDGRAQLIDNRLPRRVHEKMRERLGRLSDEASDAV
                     TVAASLGRTFTFDELARTLGQPASDLLGPVAELLEANLLVERDEKLAFWHDLTREAVR
                     ASVPVTARRALDRQAAVVLLEVGALPVEVAVQLAASAELGDEVAITTLLEAAQALVTT
                     DSGTAAQFGQRALAIAPAHHPQRGEIVATTAIALHIAGDSEQAIAFADRALRETLPAL
                     QEAEVRLSIAGMFAISPEIRIGAGRLALNLPDLPETLRARHLACLFHNLVTAGRLDEG
                     RAVLEETRAAVESGDDARAMFTLRVAESALEYTDDRFGPSHELITSAYRDGIFAGDDQ
                     RLRLAHMWHGELLSVADRDDEALAIAADGLAAAQRDRQGWAYQMFETWHGRMLLRTGR
                     ISEAAAALEGRFALEDGTHAAAVLDAAGIVALGRLAIHTGDTRQTRRLGGIAHVMLEQ
                     GTPAVRRHAGWLLALFAMAEGDAEAARGWLRTPTEPDGRGILPRFPLDIADEVVLARI
                     GLAARDGELAQLALLKSRRRSELNPGVASIAATAAHVRGLLESNHADLREAVDLFERT
                     PRRLELAAALEDLGVELIATDRESAVEALGRTLALYTELGAPWDARRVRSRLRELGVR
                     RRLVTAEPETNGWAALTTSELTVARLVAEGLTNRE"
BASE COUNT         3579 a         7418 c         7050 g         3266 t
ORIGIN      
        1 ccgcgcgacg gcgagcctct gctcgagaga gccggaacaa gcgcccgaac atctccaggt
       61 tctcgcggcc cgtcagtagc tcgtcgaccg cggcgaactg accggtcagg gcgagcagct
      121 cgcgcacgct cgccgactca ctgacgacgt cgtgaccgag caactccgcc cgtcccgcgt
      181 ccggctcgag cagcgtcgcc aggatgcgga tgagcgtcgt cttgcccgcc ccgttcggcc
      241 cgagcagacc gaaaaccatg ccctcagcaa cctcgaggtc gacgcccgcg agggccgtcg
      301 tcgagccgaa gcgcttgacc acacccccga cacggacggc gggcggccgg ctgtgccgcg
      361 atccgtcgga ctcaggctgc gacgacgacg tcgcgctgtc cgggtgaccc tgcacgttgc
      421 attacctcct ggccgacggc accgattatc gcgcccccgt cgccaggctc gccatcaggg
      481 gtgcacctgg gagccgtagc cgaggtcgag ccggatcgac tccatcaccc agaaggtcgt
      541 gctctcggtg aagacgtccg cggctgcgag cactccggtg tacttctcga tcactggtgc
      601 tgctgcgacc gccgtgcctc gcccggctct tcgagcagcg gttgcctgct cccagcccgg
      661 aaagactcga ggcgtgagcg ccacatcggt gctgcagctg cggcggtgaa agcggagcgc
      721 gcatcgcagg aagactcggt gcggacgcgc ctgtcaatcg atgttccacc gacgcagagc
      781 gccgccgctc cgaagcagga actaatactc acacttgacg agctagcgct catctacaag
      841 tcgctccaag cagtgaagac gctgggcgca ctgccattca gaggatgagc tactcgagga
      901 cacgatccag ctcgccgacc agggctgaac gacgcgattc ggtagcgagg accgatgcag
      961 ccgttcagcg gctcgtgact tgcgacgttg cctatttgga aaacaaggga tggagccttc
     1021 tggagccctg tggcgctgca ccctccgttg cgaacgtcgg gacgatgtgg tattgcgcac
     1081 gtgtagatct gagatgcgcg actgcctcca acaggaggga ctaccgagcg gcttcggacg
     1141 cgagagtcac gaagaaagga gctcagttgt cggtaactga cgagccgatc agtttcgagc
     1201 agcagatcaa acccttgttt cgggagcgcg accgtcagtc gatgacgtgg gcgttcgacc
     1261 tctggtcacg cgacgacgtc gctgcgaaca gcgaggctat cctcgacaag ctccgcgacg
     1321 gcacgatgcc ctgcgatggt gcttggtcag acgagcagat cagggtcttt caacgctggg
     1381 tcgaggcggg atcccccgcc taacctccgc gcggtggctg cgccccttcg gccgtggcct
     1441 gataccaagc gaccgtgtcg tgcacagcct gggcgagcgg tgtgatctgt aggggcccga
     1501 aggctctgtc gaatgcggag ccgtcgacga caaagggtgc ggcgaactgg taccagatgt
     1561 cggggacctc tcgggcttct gggatgccca gcccggctgc tcgcatcgcg agcttcgatg
     1621 tgaccgagag cttcaccgtc cggccgcacg cctgcccgat gagggcgacg aatgctcgcg
     1681 cggtgatcgg aggggccgcg ggaagcaccc atgccgtgcc gtttgcctcg tctctctcgc
     1741 caaggaccgc gaggccgcgc gcaacgtccg ggaggtagct gaaggtgtgc ggaaaatccg
     1801 cgttggccgg ccagcgcaca gtcttgccag cgagagcgcc tgtcaccagc atgcccgcca
     1861 cgctgtgcgc accacccggc ccgaagtagt cggaggcgcg gccgatcgtg acgcgcagac
     1921 catgattctc ggccgcggcg agaaacgacg cagcaagctg cttgcgcagg cggcccttct
     1981 tgctttgcgg ctcctgctgg gaggtctcgc tgatcgggcc gctgatcggc ccgtacatgt
     2041 agaggttgtc ggcgacgacg agctttgggc ccgcttcggt cacggccgcg acgatggtgg
     2101 cgttcagcgc cgcgaactcc tccgcccaac gggtatagcg gggctgcaca cagtggtaaa
     2161 cgaccgcggc gtcggcacaa gcgcgggccg ccccctcggc cgtgttcaga tcggcagcgc
     2221 acgcctcgac gccatcaggt acccgggcgc gtccgtcccg gctcactgcc cgaacgcgta
     2281 ggccccgtcc ggcgagctcc cacacgaccg cgttcccgat cccgccgcct gcgcctagca
     2341 ccgcacagat ttccatcgcg acgagcaagc ctattcgtcg gagaccggac gatcgtctgc
     2401 ggttccgcgc atcgcagaca cgggagtgag aagcttgcga gcagatggca cgcgccgagc
     2461 acgcccagaa ggccgcgccg tcggcgttca tcggtccgcc cgagagccgt gcgcgtggca
     2521 aggcggcgcg tggggaggcg cctcgatcga gccattccct gctccccctt gccgacgatc
     2581 gcgacgctct cgccttgctc gagtcgcagg cccaattacg cgtcccggag ctcattccga
     2641 ttcgctacgg gcgcatggtc gcctcgccgc ttgcgttctt tcgcggcgcg gcgaccgtga
     2701 tggcacacga tctcgcctcg acgcctacgg ccggattgcg ggttcaactc tcgggggatg
     2761 cccatttcct gaacttcggc ggctacgcgt ccccagaacg cgacctcgtg ttcgacctca
     2821 acgacttcga cgaaacgttt cccggcccgt tcgagtggga cgtcaaacgc ctggcgacga
     2881 gcttcgagat cgccgggcga gatcgcagct tcgcacgcac tgaacgcaag gacgccgtgc
     2941 tcgaggccgc gcgctcgtac cgagaggcga tgcggcgatt cgccgcgatg ggtgagctgg
     3001 acgtctggta tgcgcggctc gacgcgaaag cgatcgacca acagctgatg gaggcccacg
     3061 acaagaagct cgggcggaca ctccgacggg ccgaggcgaa gggacaccga cacgatcgga
     3121 tgctcgcgct cgcacgattg accaagaccg tcgacggaga acgaaggttc atctcggacc
     3181 caccgctgat cgtcccggtc gccgagctcg acgagcgcga agacgaagct ggtctcgagc
     3241 cgctcctaca cgcgctcttc cgccgctacc gtcgaacgct tccagccgag cggcagacgc
     3301 tgatcgagcg tttccgctac gccgatctcg cccgcaaagt cgtcggcatc gggagtgtcg
     3361 gtacacgctc ctgggtgatg ctgctcctcg gtcgcgacga cggagacccg ctctttctcc
     3421 agatcaagga ggcgctgccg tcggtgctcg agccacttct cgggagaagc ccgttcgcca
     3481 cacacgggca gcgagtcgtg gtgggccaac gcctgatgca ggccgcgagc gacatcttcc
     3541 tcggctgggt tcactcggac gaagagctcg acggccgggc gcacgacttc tacgtccgcc
     3601 agctctggga ctggaagacc tcggtcgacg tcgagacgat cctgccacga ggcctcactg
     3661 cgtctgccac tgcatgcggc tggacgctcg cacgtgcaca tgcgcgctca ggcgaccgaa
     3721 tcgcgattgc cgcttacctg ggagcgagcg acagattcga tcgcgccgtc gccgaattcg
     3781 ccgcggggta cgccgatctc aacgagcgag accatcgaca cctgctcgag gcagtcgccg
     3841 agggccgcgt cgacgcggcg gagggcgtgt agggcgctat gccaggtgat tcggccgatc
     3901 acctggcgtc ggcgctcagt gggggatgat cagcacgtcg atcgggaagt attgcggtgc
     3961 tccgccttca ggtggcgggc cgcccatgac ctctccaagc gccgggccaa gcttctcttc
     4021 catgaagcga tcgaaggcct cacgcgactc ccagacgtcg taaacgtagt agccctgctc
     4081 gccctggccg gcgctgtgga cgatcagccc ctccggcgcc tcatcttccc gcataggggc
     4141 ggggtgccca aacatctttt cggtgacgtc gtcgtagacc tgcttcgtga actcgggcgc
     4201 tgccgtcagg attccaaccg ccatctcgcc tcccttgagt ttcgtcggat gccggattct
     4261 aggccgcaac gctcgagctg ggcgcgctca gccgtgtgtg aggctgacca gctcgaaccg
     4321 ttcgtggccg ccctgggtca cctgctcctt gacctcgccg attccactta cgtagtactt
     4381 cttctcaaac tgcggttcga ccgggctcca ctcgatcgtg gcgagcgacc tcgtgaaggt
     4441 cccatacggc actttcacgc tcacggcgag cccgagcacg cgcccctggt ccagcgcccc
     4501 gccgggcggg taatactcct gccggtacac ctcgttcggc ctcgggttgc cgcgcatgat
     4561 gatccccggc ttggctccgt tgaccccagc cttccaggag tcatttgcgc gaacgaatcg
     4621 gccgttctgg agttctcggg cgtcctcccc catgtaccag acattgccct gcttgtcctg
     4681 cgcgtaccag tcgaacgttc gctcgagtgg tttcccatgc tgcgagaccg tgtcgcgcac
     4741 gacagtacac gtcactccca gaatccgaat ggtctgacgg gttacgacca tgtcgtcgat
     4801 ttgcggggtc ttgcctttga agcccttgta gtggaacgtc gtgccgggct tgagcgggaa
     4861 gtagcggttg tcgaccgcgg ccacaaagtt cgcggggtcg atcttcgcgg agtagctccc
     4921 gtgcagcgcc caggacggcg gcttcggctt tcccgctgtc gaagggccgg caccaagtac
     4981 cagagccgcg atgagcgcca ctccaaccat cggcagcacc agtgctcggt tcattcgtgc
     5041 cttggagttg gtagcacgat ctgtcatgtg gcccgtcctt tcgctagcac cccacgcgct
     5101 agagctcatg acgctacgga gcccagatga aatgttggtg agagcggccc tcgccacaga
     5161 ctgccatcgc gcggctacgc tcgcacgatg gccgctcccg ccatcccctc gctgtccgcc
     5221 tcgcagctga cgacactcgc cgcggcgggc gaagagagga gagcggatgt cggcgacgtg
     5281 ctgttcacgg tgggggatgc gacgtatccg ttcatcgcca tccgcgaggg cgaggtcgcg
     5341 atccgcgatg cggccggcaa cgagatcatc cgccacggcg cctcgagctt cctcggcgag
     5401 ttgaacctgc tttccgggca aaccgtcttc ctgaccgcgg tcgtgacgaa gccgctgcgc
     5461 tacgtcgcag tcgaccgcga cgcgctgcgt gcgcttctgt tcgaggacgg accgctcagc
     5521 gacctcgtcc tctcgacctt catcgcccgc cgagaggcgc tccagcgtgt ccaagggatc
     5581 ggcctcgaga tcgtcgggcc ccgctcgtcc gaggcgacga tgcagatgct cgacttcgcg
     5641 cgcgccaacc gactcccgtt cacctggcac gagatcgcac cgccggacgg aatcgagccg
     5701 ccgctcgtac gactcccaga gggcaccgag ctgcagcacg cctcgcgagg tgaggtgctg
     5761 cgtgcgctcg ggatcgggcg cgagctggcg ccgcgcgagg aggtcgacct gctcgtggtc
     5821 ggcggcggcc cggccggcct cgccgccgcc gtttacggcg cctccgaggg actcgacacc
     5881 ctgatcgtcg agggcaccgc gctcggcggc caggccggat cgtcgcgccg gatcgagaac
     5941 tacctcggct tcccggcagg gatcagcggc accgagctga ccagccgcgc cgtcagccag
     6001 gcacgcaagt tcggcgcccg acccgcgacc ccctaccgcg cgatcgcgct cgaacccggc
     6061 gacggaagac acctcgtacg actggaagag gagcacgaga tcgcagcacg cgcggttgtg
     6121 ctcgccaccg gcgccgccta tcggcggctc cccctcgacg gcctgagcga ctacgagggc
     6181 gtgagcgtgt tttacgcggc cgggccgccc gaggcgcagc agtgcggcgc ctcacgcgtc
     6241 gccgtcgtcg gcggcggcaa ctccgccgga caggccgccg tctggctcgc tcgcggcggc
     6301 gccctcgtca cgctgctcca ccgccgagcc gacctcagcg agacgatgtc cgactacctg
     6361 atccgtgagc tcgagcgcta cggggtcgcc gtccgcgacg gcagcgagat cgccgccctt
     6421 cacggaacgg aaggccaact cgcagaggtc acgctcaaga acggcgagcg cctcccattc
     6481 gccgcctgct tcttcttcct cggcgcacaa ccgtgcaccg aatggctcga cgacgccgtc
     6541 gcacgcgccg ccgacggctt catcctcacc ggcaacgcag ctggcgccga caacctgctc
     6601 gagacgagcg ttcccggtat cttcgccgcc ggcgacgtcc gctccggatc cagcaaacgc
     6661 tgcgccgccg ccgtcggaga gggatcgatg gccgtgcagt tcgtccacgc gcacctcgca
     6721 gctctcgcaa gccgagtcgg atgagcgaac tctgtactca ccttgaccag gtcgagttga
     6781 gcgagctccc ggcctccgtc gacggctgcg aggaatgcct gcgcgacggc ggcaagtggc
     6841 tgcacctacg gatctgcctc acctgcggcc acgtcggctg ctgcgacgat tcacccaatc
     6901 gtcacgcgac tgctcaccac cacgccacgt cgcatccgat catccgctcc ctcgagcccg
     6961 gcgaggactg gagctggtgc tacctcgacg aggtcgcatt cgtcctcgac ggcgttcgcg
     7021 gcacgacgcg catcccgccc tcgccgctcc tgagctgagc ccgaaagacg atcacgccac
     7081 ccggcgtgca agctgcgtgc gcgacttgac gccgagcttg cggaacacct tgcgcaggtg
     7141 gtactcgacc gtgctcgggc tgatgaacag ctgagccgcg atctcccgat tggtgtgtcc
     7201 ttgcgcgacg aggcgtgaga tctgagcttc ctgcggcgtg aggtcgccga gcgtatccaa
     7261 cgtccgcttg cgggcgcgtt cccctgtcgc ctcgagctca acccgagcgc gttccgcaaa
     7321 cgcctccatc ccgaactcgg agaagagctc gtgtgccacc cgtagctccg cgcgcgcgtc
     7381 gatccgtcgc cgttcgcgcc ggagccactc gccgtagagc aggtgcgtgc gcgcgaggtc
     7441 gacccggagg cgcgtcggcc gaagatgctg gatcgcctca cggtagagcg tctcggccgc
     7501 ttcgccgtcg gcgagcaaag cccgcgagcg cgcttcgacg ccctgcgccc agggcgtgcc
     7561 gctcgccttc gtcgtctcgc tcagcacctc gagggcgctc gccgcgcgat cgttctggcc
     7621 gctgcgtgtc gcggcctcga tcagctcgac catcgtccag ggcgaaaacc acagttcatg
     7681 ggggtcctcg gccgcctgct ccgcggcggc gagcgcgatg tcgtagcggg cgagcccgtt
     7741 gtgcagacac gcagtcgccc attgcgcgag cgtgagcccc atgccctcgc cacgtgcgag
     7801 gaagtcctcg gtcgtcgcct ggatcaatcg cgatgcgtcc ggctcgcgac cgcggaacgc
     7861 gacgagcgcg aggggcgcgt acgggacatc gcgactgtcg gtcgcatcgg tgatcgcatt
     7921 cgcttcagcg atcagcaagt tcgcgtcggc gaggttgcct gcatacagct cgactcctgc
     7981 tcgggtgctg agggtgagcg gcaggacggt cagggcaccg gattcgcgcg cgatgcgggc
     8041 ctgcgcccgc gtcagtgcat cccagttctc gtagtcccag atgtacccgg ctgcgcgacc
     8101 cgcgagccac agccaacgga gtccttcgtc tgttccgatc tcgtcgttgc cgaaggcggt
     8161 cagcgccttg cgcaggactg gcgtgcctgc cgccgccccg tcggtgatca ggaccgccaa
     8221 tccgtcgagg agaaggtccg ccccgtgcgc cggcggctcc ggcggaggcg cggcacgtgc
     8281 cgcggccgcc acttgtcgag tatcgacctc gcctccgagt cgaccagcga agagagcggc
     8341 cgtcagtgca tcgaggtaga tctggcgcgc ggcggcaccg tcgagcggct ccaggcgctt
     8401 ggccgccgtg agcaacagcg gcggcgcctc gctcccacga tccgcggcga aggagatgcg
     8461 agcgcgaatg acgtccactt gcgctcgttg tacgtcgtcg agcgggcctg cctccgcgct
     8521 cgaaacaagc gcgagcgcat cgtcgagagc gcccgcctgc tccttcgcct ccgccgcggc
     8581 gagtgcccgc gcagcgcgct gggcgcggtc gagcgtcagg acggaggagc gctcgaggaa
     8641 gaccgcagcg gcggcgaaac cgccacgcgc ctgagcgcgc gctgcggaac gctcgagctc
     8701 ggcggcgacg tcttcgtccg gccgcgacgc cgcctgcgcc cgatgccacg cgcgtcggtc
     8761 ggggtcgatc gtcggatcgg tcgcttcagc gagcgcgcgg tggacttcgc tccgctcatc
     8821 cgctcccgct gctcgataca ccgccgagcg aaccagtgga tgtcggaact cgacaccggc
     8881 gtcgaaagcc agcaggccgt ccgactcggc ggtgtgcgag gccgactccg cgattccgag
     8941 acgctgggcg gcccgccact cgagcgcggc gtccccggtc gggtcggccg ccgctacgag
     9001 cagcagacgc cgcgcgtcac gcggcagtcg tgcaagccgc cgcgcgaagc tctcctcgat
     9061 ctgagcggag agaggcagcg ccgccgggag cccgaacccg ccggcgagct gtgccggcgt
     9121 caggccgcgc ggcagctcca agagggcaag aggattgccg cgtgtctcga cgacgatccg
     9181 ctccagcacg cgctcgtcga gcctggccgg caggaccgag tcgagcagcg cccgcgcatc
     9241 ccgacggccg agaggctcga cgtgaaactc cgcgaggccg gcgagcgcgt cgatcggctc
     9301 ccgcgctgcg aagaccatcg cgatcttctc ggccagaagg cggcgggcca cgaacgcgag
     9361 cacgcgcgcc gacgcacggt cgagccattg cgcgtcgtcg acgagacaga ggagcggccg
     9421 ctcctcagcc gcctccgaca acaggttgag gaccgcgagc ccgacgagga acggattcgg
     9481 aggccggccc gcgctgaggc cgaatgcgac ctcgaaggcg tcccgctgcg gatcgggcag
     9541 gcggtccatg aggtcgaggc tcggcgagca cagctgctgg agcgccgcga acgccagctc
     9601 catctcccct tcgactccga ctgtccggac gacccggaag tccgctccca cctcgatcgc
     9661 atagtcgagc agcgcagtct tgccgacgcc cggctcgccg tacaccgcca gcaccccgcc
     9721 ttggccgtcg cgcgccgcct ccagcacgcg gtcgagcacc tcgcgctcgc gctggcgccc
     9781 gagcaactgc accccggatg ccctcgtggc catcctggga gcatgccacc agggtcatgg
     9841 actacggatt tctgggatgc gatcgcccgg ccgaggggcg acgatgcctg cgcagaatcc
     9901 gcaactcggg aaggagtcaa gttgacaacc gcaatcattg gcgttggaaa catcgggggc
     9961 gcgctggccc ggcacctcgt gggcggtggc gagtccgtcg tcttggccgc gaaggacaaa
    10021 tcacgatcgg aagcgctcgc gcaggaactc gggccgctcg cacgcgccgc ctccgtcgaa
    10081 gacgcgatcg cggacgccga cgtcgtcgtc ttcgccgtct ggctcgacac gatcaaggaa
    10141 ctgatggcaa gggatgcggg cctgctcgac ggcaaggtcg tcgtcgatcc ctcgaatccg
    10201 ctcgggttcg acaagagcgg ccagatgatt cggacactgc ccgaagaaca gtcgtccggc
    10261 tccgtggtcg ctgccctgct ccccgcgggc gcgcactacg tgaaggcatt cggcacactc
    10321 gccgcggaca cgctcgcgag cggcgcaaac cgcgagccac ggcgggccgt gctcttctac
    10381 gccaccgacg acgacgcggc tgcgacaacg gtcgaacggc tgatccgcgc tgccgggttc
    10441 gacccgttga aggcgggcgg cgtcgccgac gccggacgga tcgagatgcc cggcggcgac
    10501 cttcaccagg ccggcttgaa cggtgcaatc ctcgatctcg acgaagcggg cgccgcggtc
    10561 gcgggagagg tgccgacgtg agcgcgaccg agacgcccgc cctgccggac tatgcgccgg
    10621 tcccccgctc ggcgctcggc cctgccctga acgaccaggg ctactacgtc ggccgcgtgg
    10681 aacggaacct ctactggatc accgacggca cctaccagtc ggccttcctc accacctcgg
    10741 acggcgtcgt gctgctcgac gcgccgccca cgatcggtaa caacatccag cgcgctgtcg
    10801 acgagatcgc ggccgccaac ggcgtcagca acaaggtcac tcacctcatc tactcgcatc
    10861 accatgccga ccacgccggc gcgtcctcgc tgttcgacaa gaacgtcacc cgcatcgggc
    10921 acgaggagac gcggcagctg ctgctgcgcg acgacgaccc ggccagaccg ccgaacgagg
    10981 aaacgttcca ggatcgccgc accctcgaga tcggcggcga gcgcatcgac ctggcctggc
    11041 acggacccaa ccactcgccc gacaacatcc tcatccacct gcccgaccac gacacgctca
    11101 tgctgatcga catcgtcaat ccaggctggg cgccgatcta cctctcaaac ctgaccgagg
    11161 acatccccgg ctacgtcgag gcgccggcga acgcccttgc ctactcgtgg aagcacttca
    11221 tcggcggcca caacggccgg ctcggcacgc gggacgacgt gacgctgcac cagcagtaca
    11281 tggcggacat cgccgagagc tcgaggaagg cgatcgacac cgtcgatccg acgccctact
    11341 tccagaagta cggcgagaac gtctgggcag cgctgaaggg ctacctcgac gaagtcaccg
    11401 ccaccgcagc ggcgcccgtg atcgagaagt acaccggcgt gatcgcggcc gcggacgtgt
    11461 tcacggagag cacgacgttt tgggtgatgc agtcgatccg gctcgacctc ggctacggct
    11521 cgcaggtgca tccctgatgg cgagcctccc ggtacgcgat ccggtcggcg atcacctgct
    11581 cacgccgcag aactcggcgc tcgtggtgat cgactaccag ccgtcccagt tcgcaggggt
    11641 ccggtcgatc gatcgcgacc tgctactcga gaacatcgtc tcgaccgtga agaccgccaa
    11701 agccttcggg ctgccgatcg tccactcgac gatcaacgtc gcgaccggac ggggacagcc
    11761 aaccgtgccg gagctcgcgg agctgctcga agacaaccct cccatcgacc ggacgaccac
    11821 caacgcatgg gaggatgccg acttcgtcgc cgccgtgcgc gccaccggcc ggcgcaagct
    11881 gatcgcctgc gcgctctgga cggaaatctg catgacgttt cccgcactcg acgcgttacg
    11941 ggagggctac gacgtctatc ccgtcgtcga cgcgatcggc ggcacctccg aggaagccca
    12001 tcgcgccggc ctggaacgtg tcgtccaggc cggaggccgt ccgatcagct gggtcgcgct
    12061 cgcggtcgag ctccagcgtg actgggcgcg cgaagagacg gtggctgatg taatcgagat
    12121 cgtgctcacc gagcggctgc taaaaacatg aacgtggagt ctccatcccg cgagcgagcc
    12181 gcgattgccg atcctgaggc gcgcgagttc cttcgtcagg cggacccggt gctcgcgcgg
    12241 ctgatcgacg cgcggccgga cttccgtccg cgggcctgga tggacgagct tcctccgctc
    12301 gatgccttcg ggacgctcgt cttccaagtc gccggccaac agctatcggt cagctcgact
    12361 cgggcgatcg tctcgcatct acaggagcgg ttcggaggcc atctgccgtc gcccgccgag
    12421 ctgctcgcgg ctgatccgca ggtgccgcgc gacagcgggt tctcggctcg aaagggcgag
    12481 accttgcgcg cgcttgcgga aaggttcgtc gacggacgtc tgagcgacga ggcgctctca
    12541 cgcatgaccg acgacgaggt cgaggcagtc ctgaccgagg tgcccggagt cgggctgtgg
    12601 accgcgcgcg ggttcctgct cgtggctctc gaccggcccg acgtctttct ctcgggcgac
    12661 ctcgcgctcc ggcgcgcgat ccaacgagcg tacggcttcg accaccttcc gaccgaagac
    12721 gaagtggctc aggtgtcgga ccgctggcga ccgtatcgga gccttgcagt gagctacctg
    12781 ttcgcatccg aatacgacgg gcaaccgtga ccggctcagt gggaatcatc ggcgccgggc
    12841 ggctcgggca agcgatggcg cggaccgcgc tgcgcgccgg ccgcaccgtt gtgattgcga
    12901 acagccgcgg cccggagtcg ctggcggcgg tcgcgtcgga agtcggcgct accgcaggga
    12961 ccgtcggcga ggcagctacg gccggcatcg tcgtcatcgc tgtgccctgg gaccgagtac
    13021 ctgccgccgt gcgaggcctc gaatggaacg gccagatcgt catagacgcg accaatgact
    13081 gggctgccga cgacctgaac gggagaacgt cgagcgagct cgtcgccgag ctcgtcgccg
    13141 gcgcgcgtgt cgcgaaggcc gccaacacgc tcggcgccga agtgctcgca tcggatccac
    13201 acgaggccgg cggccagcgc gtgatcttcg tctccggcga cgacgccgac gcaaaggcgg
    13261 aggtcgtcgc gttgttcgaa gacgcagggt tcgcctcgat cgacctcggc aacctggcga
    13321 caggcggcgc gatgcagcag atccaccatc cgctcgccgg cgtcaacctg atccgactct
    13381 gaggagctct tatcggtggc cgtccatacc gagctctctc caaccgaagc tgccggccgg
    13441 ctcgccttgc gcgagctctt cgacgcgtac gcccactgcg ccgaccgccg cgacgccgag
    13501 ggccagaagg cgctcttcac cgacgacacc cgcttcgtcg tctacatgga cggagaaggc
    13561 agcgagccga cctacgtcct acaaggacga gaagcgctca cacccgtctt cgaagacctg
    13621 aatcgctacg aggtgacgac gcacttcaac ggccagagca cggtcaccat cgaggggaac
    13681 cgcgccactg gcgagagcta cacgatcgcc caccacattt acaccgaaga cggcgaccgc
    13741 aagatcatga tcgcatccct tcgctacctc gacacattca ccaagatcga ccagagctgg
    13801 tacttcgccg aacgcaagct catcctcgac tggagcgaaa cgcgatcgct gctgggatca
    13861 tgaggtaacg cgagagaccc gacagacctg cggcatcagt gcgcgcgtcg ctgtcggcgc
    13921 caagcatcaa tagcctgcag aaggcgcaac gcgccgatca ccaccaggta gagcccgagc
    13981 aggttgatca ctgcgccgag gctgcctcct ggccgcccga gcatcgcgat tccaaagacg
    14041 aacgcaacga tgctcgacaa ggcgccgagc caacgctcac ggctgctgag cggcagcgtg
    14101 gacagagcag cggcctcggc aactccgaga gcgacagccg atgcgcccac ggcgtacagg
    14161 agagcgagct gcgagatcgt gggccaggcg agtacgaccg tgcccgcgcc cgccagagcg
    14221 gcgatggcga tcaggcggtc ggcgcgcttg acgcgatcgt ggtgtcgcgc aagctccacg
    14281 atctcgactg ccgcagatgc cacgagcgtg atgccgacga tgatcccaac cgtacgaacg
    14341 gttggggtcg gcaacgcgag caggagagct cccgccgcga ccgcgacggc accgagcacg
    14401 gcgagcggta gtccggggag tcggcgctgc cattgagacc gcccgggccg cagacggtcg
    14461 gcagccgtct cgaccggagt ctccgtcggg cctggtaccg gtacgaccgt cagtcggcgg
    14521 ctcgtccacc cccgcaaggc ccacaccgtc agaaaagacg caccgactaa cgacccgcct
    14581 tcgtcgcaca ggagatccgt ctcggtgtcc cagagccctc cgacgaagtg gccgccgagg
    14641 aatttgtcct cgaaccactc aacgctctcg tagccggcgc ccacggccat gccgatcgcg
    14701 agcgtgacga tgaagatgcc gctgattcgc gcgactctcc gttcgcggat cgcgttaccc
    14761 gggtcgggga cgactccgag ccgcacgagc gcgatgtaga gcaccggcac gtacccgacc
    14821 ggggagaggc cgtggacgat cttgtcgtag tagaaccagc tgccgtagag gctgagcgcc
    14881 gtgccccagg cgatcaagct catcgcgacg atgaggccga agtcgaacca gcgcggcagg
    14941 tcgatcaccc gtgcgatcag cagcacggct gatgctgcgg tcagcgcaac cgcggtggag
    15001 cgtccctgga ccgcgaacac gatcgtcccg gcgatgaacg cgagccgaag cacatccaac
    15061 ccatcgcgga cgagccaggt ccaatccccg tacacgagcc gacggcgatc ccacgcggga
    15121 tgtgcctcgg cgagcgtcgt catttcagga cgccgatgtc gacgacaagg gggcgatgat
    15181 ccgatccgac ccctgatccg tcgtgatgac ccgtgacggc gacgctgttg ctgacaaagc
    15241 agttgtccag cggcactcgc aaggcgtagc cccacgtcgg ccagctgtag ccggcccagg
    15301 ctcggccgcc gtagaggtcg cggacgtgcg ccttcgctgc aaagtcccga agcgggcccg
    15361 accagggcgg cgtgttgaaa tcgccgcaaa caatgacgcg cttgccgaac tgcgaccggg
    15421 ctgcggcagc aagagcgtca aggtgccgca cgtggatcga gcctgcaaac gtcgtgtgga
    15481 catgggtgac gataacagcc acaggttttc ccgcgacgcg tacatgcgcg acgaccgtcg
    15541 gcggaccgtt ggacggaaac cgcgcgattt tcgcagagag cagcggcagc cggctgtaga
    15601 cgccgatccc gtacgcgtcg tcgcgcacgt cgagaatacg cacctggtag gccgggagag
    15661 ccgcgtgaag gtgccgcgcc atcggcggcg tcaactcgat caggccgagc acgtccggcc
    15721 gtgattgtgc gacgagccgg tcgaccgctg cgaagtcggt attgcccact tcgacgttcg
    15781 tgaccagcac gcgcagcgat ccctttggcc gcgccccgac ggctgttgcc gtcgccgcga
    15841 ctggaatccc catcacgacc gcgttgacgg ccgcgagcac caccgcgacg agggcgagcc
    15901 tcgatcggcg cagcatcaac gcagcaagcg ccgcagcgag gagcaggacg aggtactgca
    15961 gacggaacac gtccgcgatc tcgaacaccc agaagacacg gtccagaagg ccaagcagcg
    16021 tcgccagccc gacaacgaga acaacagcgg tggcgatgcc tcgacagcag cgcactactt
    16081 gaaaccacag catgcatgga cgttttagcg actcgtcggc ctcggggtct tcagcacgag
    16141 gcgggcaact gcggccatgc cgacggcgtt cgggtggtag ggccccctga ttagcgggtg
    16201 aatcccgttc cgccgcatct ccgccagcac gcggtcgtag aagcggaggc cctcctcgga
    16261 gaaccggccg ggctccagtt gctaccagtc gcgtggccct cgctttggaa cgccgagctc
    16321 gaaaatgcgg ctcgggccta ggccacgagc gcccgttgag gggggagccg tggatcgaga
    16381 cgatcagctg accgtcgcag tcgtcctggt agtgggggtc gattgggatt gccgttttcg
    16441 gttccgacgg cgacgcgagg catgagagtc tcgttgcttg gagtcgcgca ggcggtgggc
    16501 cgctgaaagg tcactggccc gcgagcgcgg cgcgcgcggc ggccgcgatg tcggcgttcg
    16561 tgttggcgtg accgccgatc ccccagccac cctcaggcga ctcggacagg agcacccagg
    16621 tgcgctcgcg caagctggtg tccccggccg cagccgcgac aatatccgtg agctccttca
    16681 cgacaccaag ttgcttgtcg cgatcgagta cccccaccgg ggtgagtacc tgaactcgca
    16741 cgtagttgct ctcccccgcg acattggaga tcgcggctgc atccatgtcg tgaatgaatg
    16801 cggcggtgtt gttcgcgaag agcggcagat cagggacctg ctcccaacgc atgactgccg
    16861 ccgcgaggtc acgcgcaagt ccatgcggat ccgcgaacgt cccttcggcg gcgtagacgt
    16921 cgatcatcgg catcagctcg tctcctcgag gaaggcgtgg acgtcggcgg cgaactcttc
    16981 gtggtgctgg aagagaaagc cgtgcgccga atcgggatag atcttgaccc gcgcgtccgg
    17041 caggagccct gccagcaggt gggagtagcg cgggaggatc attggatcgc tgtcgccgtt
    17101 ggcgacgaac acggggagca caatcgcggc gactcgctgg agcagtgagt ggttggggat
    17161 gccccacgcg cacacggcgt cgtactgcgc ctggcgggtc tgccacgtcg tcggttcgtc
    17221 gcggtcgcct gtcctcccga agatgcgtcc ggcggcttgc tgtcccgctt cgcggctcgt
    17281 ggtcgtcggg gcgaagaaga cctcgaggta tccctgcggg ttcgtttccg gctcaccgat
    17341 cgcgccgatg acctccggcg cccagccgtg catcccttct gctccctgcg gcgccgagga
    17401 cgccagcacg acgcgccgca gcaggtcggg gcggatgaga gcgatctctt gcgcgacgaa
    17461 gctgccgatc gagaagccga gcagatcgac ccgctgaaac tccatcgcct cgacgaacgc
    17521 gatcgcgtcg tgggccattg cttcgatggt gttcggcgtg ctccctgtcg aggcgccgac
    17581 gccgacgtta tcgaacgtga ccacgcgccg actcccggcg agcgcgtcga tgagagcagg
    17641 gtcccagttg tcgaggttgc cgcggaagtg ctgtaacagg accagcggaa catcaccttc
    17701 accgaggtcg cggtacgcgt actcgatcgc gttgccggcc gtgactagtt gggtcgaagc
    17761 gacttctgtg actgacatgt ggtccgccct tctcgtaggc ggtcagcggg cggccgcggc
    17821 gccgaccgac tcggccgcct tctcgatcag gtcgacgacc tcgccgggat gcgagaccat
    17881 cgccacgtgg ctcgcgggga tctcgacggt ggtcgcgccc atacgcgcgg cgaactggcg
    17941 ctcggcgtcc ggcggaatcg cctcgtcgtt ctgcgcgacg aggtaccagg tcggcaacga
    18001 cttccacgcc ggctcgccca tgacgtcggt gaacgcggag gacgctaggg cttgctgcac
    18061 ggcgtacagg acacgggcgc gtttcgggtc gacgtcggcg gcgaagtggt tgacgaagtc
    18121 gtcctcggag agccagccgt aaccgcgcgc gtcggtgaag agatgcgcga gcgacggtgt
    18181 gacaggtccc tgcgagagaa gcgccccgag cgactcaccc tggtcgagac cgaaggcggc
    18241 aatgtagacg aggccgacga cgtctggggc gtccgacccg agcgcggtca tgatctgacc
    18301 gccgtaggag tgtccgacga cgatcgtggg gccgttctgg aactcgagca cctggcgaag
    18361 ccgtgcgacg tcgtctgcga gtgaggtcag cgggaactgc ggcgctcgca cctggaagcc
    18421 ctctgcctgg aggcgctcga tgacgccgct ccagcaggac ccgtcagccc aggcgccgtg
    18481 gacgagaacg atgttgggcc ggccgttcac ttcgaccact cccttcgttg agttgtggga
    18541 ccaccagtgt gggcaggccc ggcgtcgctg cgcatcgcac gatcctgcga tttgaaagct
    18601 gctcgagagg tctggggtat gctcgccggg aggagcggga tgtcgacagt tccagcgcgg
    18661 gagttccatg gacgcgacgc ggaactcgcg ctgattcggg gcgagctcga gcgcctttcc
    18721 gacggggccg aagcagtggt cgtcgtcgag ggcgccgcag gcatgggcaa gagccgccta
    18781 ctcgccgagg ccgcggcgat cgctcgcagc ctcggcatca gagtcggccg cagcgcagcc
    18841 gactcaagcg agacgatggt cgagctcgca acgctcctcg cggcgctgtt cgacggaacc
    18901 gagccactgc tcgatcccgg caccctttcg acgcttcacg cgcagcccga gcaacgcttc
    18961 tggctgctgc gcgacctgca gcaactgctc gagcgagcgg cgctcgagtc accgctcctg
    19021 atcgccgtcg acgacgccca ctgggcggac gccggaacgg tcgcggcgat ccgcacactg
    19081 ccgacgcgac tgatgggatt gccgatcgcc tgggtgatcg cgcttcggcc gccacgcgaa
    19141 gcgacgccgc tcgtgcacgc agtcgaacac ctgaagcaag acggagcaca aacgatcgtc
    19201 ctcggcccac tcgatgacga cgcagtcgtg cagttggccg cggagatact cgccgcgcaa
    19261 cccgatcagt cgatcctgaa gcagctcgcg gaagccgacg gtagcccgtt cctcgtcgtc
    19321 gaaacgctcc ttggactcca ggaggaagag cggatccgcg tcgtcgacgg ccgcgcccag
    19381 ctgatcgaca accggctgcc ccgccgtgtc cacgagaaga tgcgcgagcg ccttggccgg
    19441 ctctcggacg aggcaagcga cgctgttaca gtcgccgcct ccctcggccg cactttcacc
    19501 ttcgatgaac tcgcgcgcac cctggggcag ccggcctcgg acctactcgg gccggtcgcg
    19561 gagctcctcg aggcgaacct gctcgtcgag cgtgacgaga agctggcgtt ctggcatgac
    19621 ctcacccgcg aggccgtgcg cgcaagcgtt ccggtcaccg cacgccgcgc actcgaccgc
    19681 caggccgccg ttgtcctgct cgaagtcggc gccctgcccg tcgaagttgc tgtgcaactc
    19741 gccgccagcg ccgagctcgg cgacgaggtg gcgatcacga cgctgctcga ggcagcgcag
    19801 gcgttggtca ctaccgattc gggtaccgcc gcgcagttcg gacagcgcgc gctcgcgatc
    19861 gcgccggccc accatccgca gcgcggtgag atcgtcgcga cgacggcaat cgcgctgcac
    19921 attgcaggcg atagcgagca ggcaatcgcg ttcgctgacc gcgcgctgcg tgagacgctt
    19981 cccgccctgc aggaggcgga agtccgcctc agcatcgccg gcatgttcgc gatctcgccc
    20041 gagatcagaa tcggggcggg acgcctcgcc ctgaacctac ccgacctccc cgagaccctg
    20101 cgcgctcgcc acctcgcctg cctcttccac aatctcgtca ccgccggtcg cctcgacgag
    20161 gggcgtgcgg tgctcgagga gacgcgtgca gcggtcgagt ctggggacga tgctcgagcg
    20221 atgttcacgc tccgcgtcgc cgagagcgca ctcgagtaca ccgatgaccg cttcggcccg
    20281 tcgcacgagc tgatcacatc ggcctatcga gacggaatct tcgcagggga cgaccagcgc
    20341 ctgcgcctcg cgcacatgtg gcacggcgaa ctcctgagcg tcgccgaccg cgacgacgag
    20401 gcgctcgcga tcgcggccga cggcctcgcc gccgcccagc gcgaccggca gggctgggcc
    20461 taccagatgt tcgagacctg gcacggccgc atgctcctcc ggactggacg aatctccgag
    20521 gcagcggctg cgctcgaagg ccgcttcgcc ctcgaggacg gaacccacgc ggctgcagtg
    20581 ctcgacgccg ccggcatcgt cgcgcttggg cgactggcga tccataccgg cgacacccgt
    20641 cagacacgtc gcctcggtgg catcgcgcac gtcatgctcg agcagggcac gcccgcggtg
    20701 cggcgtcacg caggctggct gctcgcgctc ttcgcgatgg ccgagggcga cgccgaagcg
    20761 gcacgaggct ggctccgaac tccgaccgaa cccgacggcc gaggcatcct gccgcggttt
    20821 ccgctcgaca tcgccgacga ggtggtgctc gcgcgcatcg gactcgccgc acgcgacggc
    20881 gaactcgcgc agctcgcgct cctcaaaagc cggcgtcgat cggagctgaa ccctggcgtc
    20941 gcctcgatcg cggcaaccgc ggcccatgtt cgcggtcttc tcgagtcaaa ccacgccgat
    21001 ctccgcgagg cggtcgatct cttcgagcgc actccgcgac ggctcgagct ggcggccgca
    21061 ctggaagacc tgggcgttga actgatcgcg acggaccgcg aaagcgcggt cgaagctctt
    21121 ggccgtaccc tcgccctcta caccgagctc ggcgcgcctt gggacgcccg acgcgtccgc
    21181 agccgcctcc gcgaactcgg cgtgcggcgg cgtctcgtca ccgccgagcc cgagacgaac
    21241 gggtgggcag cgctgacgac gtcggaactc actgtcgcac gcctcgtcgc cgaggggctg
    21301 acgaatcgag agg
//
LOCUS       DASVEU010000231         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_938767,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000231 DASVEU010000000
VERSION     DASVEU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938767"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VF225_10030"
     CDS             complement(<1..1557)
                     /locus_tag="VF225_10030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7145631.1"
                     /translation="MATGVPCEVRSLRARRPSLLGFVGSLAVAALLLVASGQAPSASA
                     ALPPGFQETIVFSGLDHPTALRFSPDGRIFVAEKNGRIKVFDGLADTTPTLFADLSTN
                     VYNFWDRGLLGLALDPAFPSQPYVYVLYTYDHELGSSAPAPRWGTPGVLSDSCPTPPG
                     PTADGCVVSGRLSRLQASGNVMVGSEQVLIEDWCQQYPSHSIGSLAFGADGALYASGG
                     DGASFNFVDYGQDGNPLNPCGDPPGNVGSVLSPPTAEGGALRSQDLRTSGDPVSLDGA
                     IVRVNPATGEGLPDNPLATSPDANARRIIAYGLRNPFRINVRPGTNEVWAGDVGWSTW
                     EEINRVVDPGGPVENFGWPCYEGQARQSGYDGANLNVCENLYAQAGAVTGPHFAYHHS
                     NKVVPGESCPTGSSSVAGIAFGFYQGGPYPASYDGALFFADYSRDCIWAMRKDANGIP
                     APGLLETFVAPAANPVDVQISPAGELFYADFDGGTIRRIRFVSANQPPVAVATANPTS
                     GAAPLTVNFDG"
     gene            1874..2911
                     /locus_tag="VF225_10035"
     CDS             1874..2911
                     /locus_tag="VF225_10035"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7145632.1"
                     /translation="MKVLVTGHHGYIGSVAAPMLKEAGHDVTGLDTFLYEGCDLLDDT
                     LDLPTLRMDIRDVTSDVLEGYDGIVHLAALSNDPLGELDEDLTREINFRGTVELARKA
                     KEAGVRRFVFASSCSMYGASGTDELMTEEAPLRPLSAYAESKVRAEEALSELADGEFA
                     PTFMRNATAYGASPRVRLDVVLNNLSAWAFTTGKIMIMSDGTPWRPLVHVRDIAAAAA
                     ATLAAPIALVGNEAFNVGANDENYRVRDLAEIVRETFTGCEIEYAEGAGPDPRSYRVD
                     FGKLTETLPDARPEWTARDGARELLDAFRAARLTSAGFDLYTRLSRLKSLLARGALDD
                     DLRWSYTRERT"
     gene            complement(2915..>3567)
                     /locus_tag="VF225_10040"
     CDS             complement(2915..>3567)
                     /locus_tag="VF225_10040"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145633.1"
                     /translation="NRTVIAPLTALGPNPPLERTGLLVAGQPRYLFVGRFIERKGIDV
                     LLAAFRHVDSGELWLAGDGPLRSLVEGEAKRDPRIRFLGYADEESLPELYRQADILMV
                     PSLFEAWGLVVHEGLAYGLPVIATDQVAAADDLVNSGINGYVVPAGSHEALAEAMGSV
                     AEWSSAQWSDAAAHTRETLPKYGIDPAAEAFVRGCLLGLQHRRGLNAPGHAAGAGM"
BASE COUNT          601 a         1159 c         1233 g          574 t
ORIGIN      
        1 gccgtcgaag ttgacggtca gcggcgccgc tccgctggtc ggattcgcgg tcgcgacggc
       61 gaccggcggc tggttggcgc tcacgaaccg gatgcgacgg atcgtgccgc cgtcgaaatc
      121 ggcgtagaag agctcgccgg ccggtgagat ctgcacgtcg acggggttgg cggccggtgc
      181 gacgaaggtc tccaggaggc cgggcgccgg gatcccattg gcgtccttcc tcatcgccca
      241 gatgcagtcg cgtgagtagt ccgcgaagaa gagcgctccg tcgtaggagg ccgggtaggg
      301 ccctccctga tagaagccga aggcgatccc cgccaccgag gagctccccg tggggcacga
      361 ctcgcccggg accaccttgt tgctgtggtg ataggcgaag tgcgggccgg tcaccgcgcc
      421 cgcctgtgcg tagaggttct cgcacacgtt caggttggcg ccgtcgtagc cggactgccg
      481 cgcttggccc tcgtagcagg gccagccgaa gttctcgaca ggcccgcccg gatccaccac
      541 gcggttgatc tcctcccagg tgctccagcc gacgtcgccg gcccagacct cgttcgtgcc
      601 agggcgcacg ttgatccgga aggggttacg gaggccgtag gcgatgatcc ggcgcgcatt
      661 cgcatccggg ctcgtggcga ggggattgtc gggaaggccc tcgcccgtcg ccggattgac
      721 acgcacgatc gccccgtcca gggaaacggg gtcgccgctc gtacgcagat cctggcttcg
      781 cagcgcgccg ccttccgcgg tcggcgggct cagcacggac ccgacgtttc cggggggatc
      841 cccgcatggg ttgagcgggt tcccgtcctg gccgtagtcg acgaagttga agctcgctcc
      901 gtcgccgccg ctcgcataca gcgccccgtc cgcaccgaac gccagcgacc cgatcgagtg
      961 gctcggatac tgctggcacc agtcctcgat cagcacctgc tcgctcccga ccatcacgtt
     1021 tccggacgcc tggaggcggg agagacggcc gctcacgaca cagccgtcag ctgtgggccc
     1081 cggcggcgtc gggcacgaat cggagagcac gccgggcgtg ccccagcgcg gcgcgggcgc
     1141 agagcttccg agctcgtggt cgtacgtgta gagcacgtag acgtacggct ggctcgggaa
     1201 ggcggggtcg agcgcgagtc cgagcaggcc gcgatcccag aagttgtaga cattcgtgct
     1261 gagatcggcg aagagcgtcg gcgtcgtgtc ggcgaggccg tcgaacacct tgatccggcc
     1321 gttcttctcc gccacgaaga tccggccatc gggcgagaag cgcagggccg tcgggtggtc
     1381 gagcccgctg aagacgatcg tttcctggaa ccccggcgga agggcagcgc tcgcgctcgg
     1441 cgcctgcccg gaggccacga ggagcaaggc cgcgaccgcg aggctcccaa cgaagccgag
     1501 gagtgaagga cggcgcgcgc ggagtgagcg cacctcacag ggaacgccgg ttgccacagt
     1561 gtctcctctc gacgccacta tgccgcgggg tggtgcgccg cagcgcagcc cgcggcgtga
     1621 ctatgcgctc gacaagtgcg ctttgcaacc cagaggagtc ggcgcggtcg aaggaccacc
     1681 catacgtcta ggttcgcggc gaggccaccg agtgggtcgg gggcaacgag cttgacataa
     1741 tgagacatgg attctgagag cggagagcgc gttccgtggc cataggcgcg tccgtgggga
     1801 atcagcctcg tccgagagaa accacgacac gcctagcggt ctacgtccca atgagcgcgg
     1861 acgcgctcct cggatgaaag tcctcgttac cgggcaccac ggctacatcg gctctgtcgc
     1921 ggcgccgatg ctcaaggagg ccggtcacga cgtgaccgga ctggacacgt tcctctacga
     1981 gggatgcgac ctcctcgacg acacgctcga cttgcccacc cttcgcatgg acattcgcga
     2041 cgtcacgagc gacgtgctcg agggatacga cgggatcgtc cacttggcgg cgttgtcgaa
     2101 cgatccgctc ggcgagctcg atgaagactt gacgcgagag atcaacttcc gcggcaccgt
     2161 ggagcttgcg cggaaggcga aggaggccgg cgttcggcgg ttcgtcttcg cctcgtcttg
     2221 cagcatgtac ggagcctctg gaacggacga gctcatgacc gaggaggcgc cgctccggcc
     2281 gctgtcggcg tatgcagagt cgaaggtgcg agcggaggag gcgctgtccg agctcgcgga
     2341 cggtgagttc gccccgacct tcatgcgcaa cgccaccgcc tacggtgcct cgccgcgtgt
     2401 tcgtctcgat gtcgtgctca acaacctctc cgcctgggcg ttcacgaccg gcaagatcat
     2461 gatcatgagc gacgggacgc cgtggcggcc tctcgttcac gttcgagaca tcgctgcggc
     2521 agcggcggcc acattggctg ctccgatcgc actcgtcggg aacgaggcat tcaacgtggg
     2581 cgcgaacgac gagaactacc gcgtccgcga tctggccgag atcgtgcggg agacgtttac
     2641 gggctgcgag atcgagtacg cggagggagc gggccccgat ccacggagct atcgcgtcga
     2701 ctttgggaag ctgaccgaga cgctgccgga cgccaggcct gagtggacgg ctcgggacgg
     2761 cgctcgagag ttgctcgatg ctttccgcgc ggctcggctt acttccgctg ggttcgacct
     2821 gtacacgcgg ctctcgcgcc ttaagtccct actcgcccga ggtgcgctcg acgacgacct
     2881 gcgctggtcg tacacgcgcg agcgtacgta gatctcacat acctgcgccg gccgcatgac
     2941 caggcgcgtt gagtccgcga cggtgctgaa ggccgagaag gcaacctcgg acgaacgcct
     3001 ctgccgccgg gtcgatcccg tacttcggaa gcgtctctct ggtgtgcgcg gcggcgtcgc
     3061 tccattgcgc agacgaccac tccgcaaccg agcccatcgc ctccgcgagc gcttcgtggg
     3121 aaccggcagg gacgacgtag ccgtttatgc cagaattgac gagatcatcg gcggctgcga
     3181 cttgatcggt ggcgatcacg ggcaacccgt atgcgagccc ctcgtgaacg acgagacccc
     3241 atgcttcgaa gagcgacggc accatgagga tgtccgcttg gcggtacagc tcaggtagcg
     3301 actcctcgtc tgcgtatccg aggaagcgaa tacggggatc tctcttcgcc tctccctcca
     3361 cgagggaacg gagcgggccg tcaccggcca accagagctc cccagaatcg acgtgacgga
     3421 acgcggcgag caacacgtcg atcccttttc gctcgatgaa gcgtccgacg aagagatagc
     3481 gcggctgccc cgctacgagg agacccgtgc gttccagtgg agggtttggt ccgagcgcgg
     3541 tgagtggtgc gatcacggtg cggttcg
//
LOCUS       DASVEU010000232         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1328684,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000232 DASVEU010000000
VERSION     DASVEU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328684"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VF225_10045"
     CDS             <1..944
                     /locus_tag="VF225_10045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145634.1"
                     /translation="ARTERDFRSSRSGAADVSVFHEFHPPPYGGGNQFLLALVAELRR
                     RGLAVEVNRLSGRTPACLYNSFNFDFRRLRRFARDGARMVHRVDGPIGVYRGFDDGTD
                     RRIVKINHDLAVATIFQSRYSLDMHRELGFDLRNPVVIPNAVDPAIFYRPPAREPLAG
                     RRLRVITTSWSANPRKGAELLRWLDRNLDFDLYEATFAGNTQATFERIGVVGPLASEP
                     LADLLRAHDIYLAVSRDDPCSNALLEGLACGLPAAFLRSGGHPELVGKAGIGFDDPEE
                     LPEVLARLAAEIEERRAAIRVPALSEVADRYSEVLRA"
     gene            941..2059
                     /locus_tag="VF225_10050"
     CDS             941..2059
                     /locus_tag="VF225_10050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145635.1"
                     /translation="MTTALRRLVSAPSRLALGAYVPARTATWPESSRLFVLGDDIGWS
                     IDDDRARLTATARRLGYEVPPSSWAGFAKKQAVFHPSHFDALRPRWTESSHRLGLSYF
                     HGRPGTDGYPEFDQAFDLLRRHADRIERVQVTHAEMHELAIAAGVASNAVFRIPIGVE
                     VERFPLGDATTRGQARGALGLPESAFVVGSFVKDGVGLGEGSEPKLLKGPDILVTTLD
                     RLRNSIPELFVLLTGLARGYVRRELERLGIPHHHVYLRSRDELARAYHALDVYLVTSR
                     QEGGPKASLEAPAAGVPLVTTRVGQAQELLVDEVEALQADVDDVEALSTAVQRVHDDA
                     ALVSRLRVAARAKAETYSDERLDTRWERLLDGFVHRVD"
BASE COUNT          317 a          741 c          722 g          370 t
ORIGIN      
        1 gcgcgcgaac cgaacgtgac ttcaggtcat cgcgcagcgg cgccgcggac gtctccgtct
       61 tccacgagtt ccacccgccg ccgtacggcg gcggcaacca gttcctgctt gcgctcgtgg
      121 ccgagctcag acgtcgtggg cttgcggtcg aagtgaatcg tctctcgggc cgcacgcccg
      181 cctgcctcta caactcgttc aacttcgact tccgtcggct ccgccgcttc gcccgagacg
      241 gggcacgcat ggtccaccgc gttgacggcc ccatcggcgt ctatcgcgga ttcgacgacg
      301 gcaccgaccg gcggatcgta aagatcaacc acgatctcgc cgtcgcgacg atcttccagt
      361 cgcgctacag cctcgacatg caccgcgagt tgggattcga tctgcgcaac ccggtggtga
      421 tcccgaacgc ggtcgacccc gcgatcttct atcgaccgcc cgcccgcgag ccgctcgccg
      481 gccgccgtct gcgcgtgatc acgaccagct ggtccgcgaa cccgcgcaaa ggcgccgaac
      541 tgcttcgctg gctcgaccga aatctcgact tcgacctcta cgaggctacg tttgccggca
      601 acacccaggc gacgttcgaa cggatcggag tcgtggggcc gctggcgtcc gagccgctcg
      661 ccgatctgct gcgcgcacac gacatctacc ttgccgtgag tcgcgacgac ccctgctcga
      721 acgctctgct cgagggcctc gcctgcggtc tgccggccgc cttcctgcga agcggcgggc
      781 atcccgagct ggtggggaag gcgggaatcg ggttcgacga cccggaagaa cttccggagg
      841 tgctggcgcg actcgccgcg gagatcgagg agcgccgcgc ggccattcgc gttcctgctc
      901 tctcggaggt ggccgacagg tattcggagg tgctgcgcgc gtgacgacgg cgttgcgacg
      961 ccttgtctcc gcgccctcgc ggctggcact cggcgcctac gtcccggcca ggacagcaac
     1021 gtggcccgag tcgtcgcggc tcttcgtact cggtgacgac atcgggtggt cgatcgacga
     1081 cgatcgggcg cgcctgacgg cgacggcgcg acggctcggc tacgaggtcc cgccatcgtc
     1141 ctgggcgggc ttcgcgaaga agcaggccgt gttccatccg agtcacttcg atgcgctgcg
     1201 tccgcgctgg accgagtcgt cgcaccggct gggcctcagc tacttccacg gccgccccgg
     1261 gaccgacggg tacccggagt tcgaccaggc cttcgacctg ttgcgtcggc atgccgaccg
     1321 aatcgaacga gtgcaggtga cgcacgcgga gatgcacgag ctggcaatcg cggccggagt
     1381 cgcttcgaac gccgtctttc gcattccgat cggggtcgag gtcgagcgct tcccgcttgg
     1441 cgacgcgacg actcggggcc aggcacgagg ggcgcttggc ctcccggagt cggccttcgt
     1501 cgtcggctcg ttcgtcaagg acggggttgg tctaggcgaa ggatccgagc caaagctgct
     1561 gaaggggccc gacattctcg tgacgactct cgaccgcctg cgtaattcga ttccggagct
     1621 cttcgtgctc ctgacagggc ttgcgcgggg ctatgtccgc cgcgagctcg agcggctggg
     1681 gattccgcac caccacgtct atctacgctc ccgcgacgag cttgcgcgcg cctatcacgc
     1741 gctcgatgtc tatctggtca cctctcggca ggagggtggg ccgaaggcat cgctcgaggc
     1801 tcccgcggcc ggcgtcccac tcgtgacgac gagagtcggg caggcgcagg agctgctggt
     1861 ggacgaagtc gaggccttgc aggcggacgt cgacgacgtg gaggctttgt caaccgcagt
     1921 gcagcgcgtc cacgacgatg cggcgcttgt ctcgcgactg cgcgtcgccg ctcgcgcgaa
     1981 agcggagacg tacagcgatg agcgactcga cactcgctgg gaaagacttc tggatggctt
     2041 cgtgcaccgt gtcgattgac acggcgcgcg caggtcggta cgcccgggca gccaggcgct
     2101 gggcgcgctt gcttgcgcct cgacgtcccg agccgggcct acgtgtcttc
//
LOCUS       DASVEU010000233         9296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_165577,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000233 DASVEU010000000
VERSION     DASVEU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165577"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF225_10055"
     CDS             complement(<1..717)
                     /locus_tag="VF225_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145636.1"
                     /translation="MSVSYELVTHNAAAKERTRQYASEEPLSPGEVLQLGGRFWLVES
                     IDQPDGESEVPRAIAKPARYRLRLRHPDEREELGALRRFRAGSPRLGHAFTTLETGQP
                     ISWQVVEEQLARADDGDPFLDLIAERDYAEAEGELPDHELEHALERERDEGLPSGAAT
                     AIARAVEEGLDVELVALDPGEEPDWPESLRYIDALIFEEIEDDLFEQCGVDVAHDRRE
                     TWLGIVKERLRADLEAFRNDV"
     gene            complement(831..1289)
                     /locus_tag="VF225_10060"
     CDS             complement(831..1289)
                     /locus_tag="VF225_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7145637.1"
                     /translation="MSQLLPERNRTGSENWSPLSELGQLNERMRRMLDQTFGGMLDEP
                     AGWIPAVDIEELEDAYVVEAEVPGVNRKDVNIEVSGNELTISGEIKERKREGIIRRRA
                     RRVGQFEFRVVLPSEVNPEGVDAKLNDGVLTVRIPKAAGAQRRRIEVKSA"
     gene            complement(1331..1798)
                     /locus_tag="VF225_10065"
     CDS             complement(1331..1798)
                     /locus_tag="VF225_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7145638.1"
                     /translation="MSASSHDGVAVLRKSDLSKVPTPCRSDLDLIERVDQALDVLQGK
                     WKVHLLFFMARGVRRHGRLLACLPGVSKKVMTDTLRALERDGLVARRIFAEVPVRVEY
                     SLTPLGWSMTEPLIALSEWGDLHSREVTEAQAQYRNGSASLGSSRIAQRSAAA"
     gene            complement(2007..4724)
                     /locus_tag="VF225_10070"
     CDS             complement(2007..4724)
                     /locus_tag="VF225_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX7145639.1"
                     /translation="MLLNREQELQFIDGLLADVRVGEGGALVLRGDPGIGLTTLLEYA
                     GGAASDMRVVRVPGVETEFHLAFAGVQQLCAPLLDGLDALPDRQRAALESALGLNGGD
                     SPDVFLVGLAVLTLLGHAAGEEPCLYLFDDAQWLDEASVEAIAFVARRLDAEPIAFLF
                     GVHEPTMSRVPLDGISDFRVVGLAAEESRELLRRVVAGPLDQSVRDRLIAETDGNPLA
                     LVELPAELTADQLAGIATLPETLPLGVRLEQYFLNQFRQLPAPTKMLLLLLAVEPGRD
                     PNVFWRAAANLGISAEAAGAAVDAGLLKITPRVRFRHSLMRSAISADASVLDLQRAHR
                     AYVDVIDADVDPDRRVWHRAGASSGPDEEIAHELEVSAARAKGRGDNRAAAGLLQRAA
                     ALTPDPARRGGRMLAGAQAELAAANAGRATALLGQATELPLSDLERVQAARLRASIGF
                     ALGQGADSAVMLLEAARALESLDVRAAREAYLEALEAAVYSGRYGNGGAIRKAAAAAR
                     AGPPAPKPEIAADLLLDGVAELLLDNRDRGVPVVQKAIEALKQTPELRWQAFGCLAAM
                     EIWDDTGLHTLATTQLVLAREAASLTPLTIALDQIAGGDNVVSGQFAVAEANFAEAQE
                     LAAATGEAGIASHASAGALIVSAWRGRTPEARDLAEANMRDAIARGLGRYVTLTQYAT
                     AVLANGLGHYEEALGAARSAAQDQALYLSTFALPELIEAAVRGGEHELADAAADQLTE
                     RTQSGTEWARGMLARSRALISSDGEAEPLYRAAIKHLQNCRAAPHLGRAHLLYGEWLR
                     RQNRRRDARSQLRTAHEMFRFMGAEAFGDRARLELVATGERVRRRQVGTEDLLTPQEA
                     RIARLVADGASNPQIAAQLFISPRTVEYHLHKVFRKMGVGSRADLMRLP"
     gene            4824..5801
                     /locus_tag="VF225_10075"
     CDS             4824..5801
                     /locus_tag="VF225_10075"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7145640.1"
                     /translation="MTTILQDSYAGPGARHEALLYTSADEFASLTAPFVAEGLDKDES
                     AVVVTSAENLEAFQAALAEPSPLVLLADGEDWYRNPAETIGRWVGFVEGQVARGRSRV
                     RGVGEIAPLRRDQAVEQWLQYESILNTLLAPLPLSVLCAYNTEVLPESIIEGVRKSHP
                     TVIEDGSASASESYLPPSGPPTRPLALDDRQTLTHQPGDVADTCTYVEAEARRAGLAE
                     WQMQQLLAAAGEVAHNAFLHGSAPVFVTAWEEHESFVCQIEDDGGGIVDLAAGYMPPT
                     NGNDGWGLWLARQLTDSLEIGRGPYGSAVRLTMHPSRPKPPRGTLPTRL"
     gene            5922..8630
                     /locus_tag="VF225_10080"
     CDS             5922..8630
                     /locus_tag="VF225_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7145641.1"
                     /translation="MAAADQPVCPRHGRPAVASRVRLRPDGTQEVEYLCEIDLAEEQM
                     SSRFGGRGGSLFDDFFSDFFGDGQSSPGGGARRPAAAQRQVERVDVTQFFSDATRELL
                     QRAAQTAVEWGSLDLDSEHLLYAALQDDVVRHVLRQIDADAEAIAAEIEDEADKVSRT
                     DVSPSLSPDAKAALLAAYEESRELGSSYVGPEHVLLALARDDESEAGQLLQRFGVSHT
                     KLRGAVIRGVEAGGGTRETSKTPTLDQYGRDLTQMAREGKLDPVIGRADEIEQTIEIL
                     SRRTKNNPALIGDPGVGKTAIVEGIAQRIVNDEVPETLTDRRVVQLDLAGMVAGSKYR
                     GEFEERLKKVIDEIVENEGNIILFVDELHTLVGAGAAEGAMDAGNMLKPALARGEMHV
                     VGATTLDEYRKDVEGDPALERRFQPVFVREPTVDETIEILHGRKDRFEAFHRVRISNE
                     AIVAAAELSDRYIRDRFLPDKAIDLIDQASARVRLRNRTKDDDTRNLEEDLRRLARER
                     DQATTAEDYDRAGQLTDQMESRRGELDERQKRRQRAPEVTAEDVAVVVSRATGIPISQ
                     LTTEERQRLLDLEQQLHGRIVGQEEAVKAVAEAVRRSRAGLGDPRKPIGSFLFLGPTG
                     VGKTELARTLADALFGSEDLMVRFDMSEFQERHTVSRLVGAPPGYVGYEEAGQLTEQV
                     RRRPYSVLLFDEIEKAHPDVFNILLQILDDGRLTDAQGRTVDFKNTVVIMTSNLGADR
                     IQQQARTNEPFEQLKADLMQILRQSFRPEFINRIDEIIVFRALTEEQLVDITQLLLDR
                     LERRLRAQHIELEFTQEAVRLLAKEGYDPEFGARPLARTIQRLVENELSRMVLSGEVE
                     PGDKVQVEADGDELKFDVERGAASQEVTAWSEESEAAQTPEPAAAR"
     gene            8678..9289
                     /locus_tag="VF225_10085"
     CDS             8678..9289
                     /locus_tag="VF225_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7145642.1"
                     /translation="MAKRVLDLIVAAALVALLSPLILILLVALAADMIVSRRDRGGFF
                     YREPRISRGRLFGLLKFRTLRRDVLDELRAAGGHARLYEADPANLTWVGKRLLKPWYL
                     DELPQLLNVLRGDISLVGPRPWPPEMVRRQVAEGLDYRNRMLAGLTGPAQVTKGEGIA
                     YEDLDSRYLQCQSTLSSWALVRYDLRILWRTAAVIARGEGLNY"
BASE COUNT         1573 a         3149 c         3079 g         1495 t
ORIGIN      
        1 gacgtcgttg cgaaatgcct cgagatccgc ccgcaggcgc tccttcacga tgccgagcca
       61 ggtctctcgc cggtcgtggg caacgtcgac cccgcactgc tcgaacaggt cgtcctcgat
      121 ctcctcgaag atgagtgcgt cgatgtaacg cagcgactcc ggccagtcag gctcttctcc
      181 ggggtcgagt gcgaccagtt cgacatccag cccttcctcg acggctcgcg cgatcgccgt
      241 cgcggcgccc gaagggaggc cttcgtcgcg ctctcgttcc agcgcgtgct cgagctcgtg
      301 gtccgggagc tcgccctcgg cttccgcgta gtcacgctct gcgatcaggt cgaggaacgg
      361 atcgccgtcg tcggctcggg cgagttgctc ctcgaccacc tgccagctga tcggctggcc
      421 ggtctcgagc gtggtgaatg cgtgcccgag ccgaggcgag cccgcccgga acctgcggag
      481 ggcgcccagc tcctcgcgtt cgtcgggatg cctgaggcgg agccgatagc gcgccggctt
      541 cgcgatcgcg cgcggaacct cactctcccc gtctggctga tcgatgctct ccaccagcca
      601 gaaccgcccc ccgagctgga gcacctcccc cgggctgagc ggttcctccg acgcgtactg
      661 acgggtccgt tccttcgcgg cagcattgtg cgtcaccagt tcgtagctca ccgacatgct
      721 tgggctcatt ctgcgggagg aagcaccagc tacacaagct cggctgatca ggccgacaca
      781 ggtgctagaa gcgaggggcc ggcggtggcc ggcccctctc agtggccccc tcaggccgac
      841 ttgacctcga tgcgtcgccg ttgcgcaccc gccgccttgg ggatgcgaac cgtgagcaca
      901 ccgtcgttga gcttcgcgtc gaccccttcc gggttgactt cgctcggaag cacgacccgg
      961 aactcgaact ggccgacccg gcgcgcacgc cggcggatga tgccttcgcg cttgcgctcc
     1021 ttgatctcgc cgctgatcgt cagctcgttg ccggaaacct cgatgttcac gtccttgcgg
     1081 ttgaccccag gcacctcggc ttcgacgacg taggcgtcct ccagctcctc gatgtcgacc
     1141 gccgggatcc atcccgccgg ctcgtcgagc atgcccccga aggtctgatc gagcatccgc
     1201 cgcatacgtt cgttcagctg gccgagctcg ctcagcgggc tccagttctc ggatccggtc
     1261 ctgttgcgtt ctggcagaag ttgcgacatc tatcgatctc ctctcactcg gtctccattt
     1321 cgtttgccgc tcaggccgcc gcggagcgct gggcgatccg tgaactgccg aggctcgccg
     1381 acccgttgcg gtactgggcc tgcgcctcgg tcacctctct cgaatggagg tcaccccact
     1441 ccgacagcgc gatcaggggc tccgtcatcg accacccgag cggcgtaagc gagtactcga
     1501 cccggaccgg gacctcggcg aagattcgcc gtgcgacgag accgtcgcgc tcgagtgcac
     1561 gcaacgtgtc ggtcatgacc ttcttggaca cgcccggcag gcacgcgagc aggcggccgt
     1621 gacggcgcac gccgcgcgcc atgaagaaca gcaggtgcac cttccacttg ccctgcagga
     1681 cgtcgagcgc ctggtcgacg cgctcgatca ggtcgaggtc actgcggcaa ggggtcggca
     1741 ccttggacaa gtcggacttg cgaagaactg cgaccccgtc gtgactgctg gcggacacgc
     1801 ttcacctctc tcccaatcgg tgacagaacc agtgccgttt cgactaggcg agcatccgac
     1861 agccaccggg gtggtcgcgt ccgtgacctc cctagtcatg tctcgagctg gagcaagcgg
     1921 attcgcaagg ctcctgcaga ctggtggctg gaatctgcgg cttcttgcgt cccgcccggc
     1981 ctagtggagc tgctcttcct cttggctcag gggagtcgca tgagatcggc gcgcgagccg
     2041 acacccatct ttcggaatac cttgtgcagg tgatactcga cggtacgggg gctgatgaac
     2101 agctgcgcgg caatctgtgg gttcgacgca ccatccgcga cgagccgcgc aatgcgcgcc
     2161 tcctgaggtg tcagcagatc ctccgtgccg acctgtcgcc ggcgcacccg ctcgccggtc
     2221 gcgaccaatt ccaggcgagc gcggtcgccg aaggcctcgg cccccatgaa ccggaacatc
     2281 tcgtgcgcgg tccggagctg gctgcgggcg tcgcggcgtc ggttttgtcg ccgtagccac
     2341 tcgccataga ggaggtgcgc acggccgaga tgaggcgccg cccgacagtt ttggagatgc
     2401 ttgatcgccg cgcggtacag cggctcggct tctccgtcgg agctgatcag ggcacgggac
     2461 cgagcgagca tgccgcgggc ccattcggtg ccgctttgcg tgcgctctgt gagctggtcg
     2521 gccgctgcgt cagcaagctc atgctcgccg ccgcgtacgg ccgcctcgat gagctccggc
     2581 agggcgaagg tactgagata cagcgcctga tcctgggcgg ccgagcgcgc tgcgcccagc
     2641 gcctcctcgt agtgaccgag accattcgcc agcacggccg tggcgtactg cgtaagcgtg
     2701 acgtaacggc caagcccgcg tgcaatcgcg tctcgcatgt tggcctctgc aaggtcgcgt
     2761 gcttcgggcg tccgtccgcg ccaagccgag acgatcaggg ctcctgcgct cgcgtggctt
     2821 gcaatgcccg cctcccccgt cgccgcggcc agctcttggg cttcggcgaa gttcgcttct
     2881 gcaactgcga actggccgct gacgacgtta tcgccgcccg cgatctggtc gagggcgatt
     2941 gtcagtggcg tgagcgaggc ggcctcgcga gcgagcacga gctgcgtcgt cgccagtgtg
     3001 tgcagtccgg tgtcatccca gatctccatc gctgcgaggc agccgaatgc ctgccagcgc
     3061 agttccggag tctgtttcag cgcttcgatc gccttctgga cgaccggcac gcccctgtcc
     3121 cggttgtcca ggaggagctc ggcaacgccg tcgagcagaa gatctgcggc gatctccggc
     3181 ttgggcgccg gcggcccggc acgcgccgcg gcggccgcct tgcgaatcgc cccaccgttg
     3241 ccgtagcgac cgctgtaaac cgctgcttcg agagcttcca gatatgcctc gcgtgcggcg
     3301 cgcacgtcca gcgactcgag cgcgcgtgca gcctcgagca acatcaccgc gctatcggca
     3361 ccttgtccga gcgcgaagcc gatcgaggct cgcaatctcg ctgcctgcac tcgttcgaga
     3421 tcgctcagcg ggagctcggt cgcctgcccg agcagcgccg tcgcccgacc cgcgttcgcg
     3481 gccgcgagtt cggcctgcgc accggcaagc atgcgcccac cccgccgcgc cggatccggc
     3541 gtgagcgccg cagcccgctg gagcaggcct gcggcagctc gattgtctcc gcgccccttt
     3601 gcacgcgcgg ccgacacctc cagctcgtgc gcgatctcct cgtcggggcc ggacgaggct
     3661 ccggcgcgat gccacacccg gcgatcagga tcgacgtctg catcgatgac gtcgacatat
     3721 gctcgatggg cacgttgtaa atcgagcacc gatgcgtccg ccgagatcgc cgaccgcatc
     3781 agggaatgcc ggaaccgcac gcgcggggtg atcttcagaa gacccgcgtc aaccgcggcg
     3841 cccgcagcct cggccgaaat tccgaggttc gccgcggcac gccagaagac gttcggatct
     3901 cggcccggct cgacggccag aagcaagagc aacatcttcg tgggggcagg taactgccga
     3961 aactggttca agaagtactg ctcgaggcgg actcccaacg gaagcgtttc aggcagcgtg
     4021 gcgattccag ccaactggtc ggcggtgagt tcggccggca attcgaccag tgcgagcggg
     4081 ttgccgtcgg tctcagcaat gaggcgatct ctgacgctct ggtccaaggg ccccgccacg
     4141 actcggcgga gaagctctcg gctctcttcg gctgcgagcc cgacaaccct gaagtccgag
     4201 atgccgtcga gcggaactcg cgacatcgtc ggctcgtgca cgccgaagag aaaagcgatc
     4261 ggctccgcgt caagcctgcg agcgacgaac gcgatcgcct cgactgatgc ctcgtccagc
     4321 cactgtgcat cgtcgaagag atagaggcac ggctcttcgc cggctgcgtg gccaagaagc
     4381 gtgagaacgg cgaggcccac gagaaagacg tccggcgagt cgccaccgtt gagcccgaga
     4441 gcggactcga gagccgctcg ctgccgatcg ggtaaggcgt cgagcccgtc gagcagagga
     4501 gcgcagagtt gctgcacacc ggcgaaggcg aggtggaact cggtctcgac gccgggcacg
     4561 cggacaacgc gcatgtccga agcggccccg ccggcgtact caagcaacgt agtcagcccg
     4621 atccccgggt cgcccctcag tacgagagcg ccgccctccc ccacccgcac atcggcgaga
     4681 agaccgtcga tgaactggag ctcctgctcc cggttgagca gcactgtagg gagactactc
     4741 ctgggggagc cgtaggttcc cggaacacct ggaaaacgca aaaacggacg ctactctgtc
     4801 ggagcggcgg cacgtcccgg accatgacga cgatccttca agactcttac gcagggccag
     4861 gagctcggca tgaggcgctc ctctacacct cagccgacga gttcgcttcc ttgaccgcgc
     4921 ccttcgtcgc cgagggtctc gacaaggacg agagcgccgt ggtcgtcacc tctgcggaaa
     4981 atctcgaggc atttcaggcg gcgttggccg agccttcgcc gctggtgttg ctcgccgacg
     5041 gcgaagactg gtatcgcaac ccggccgaga ccatcggccg ctgggtgggg ttcgtcgaag
     5101 gacaggtcgc gagaggacgg tcacgcgtac gcggcgtcgg ggagattgcg cctcttcgac
     5161 gcgaccaggc cgtggagcag tggctgcagt acgagtcgat cctgaacacg ttgctggcgc
     5221 ccctaccgct gtcggtgctc tgtgcgtaca acaccgaagt cctgccggaa tcgatcatcg
     5281 agggggttcg taagtcacat cccaccgtga tcgaagatgg atccgcgtcg gcgagcgagt
     5341 cgtacctgcc gccgagcggg ccacccacac ggcccctcgc gctcgacgat cgccagacgt
     5401 tgacccacca acccggggac gtcgctgaca cctgcaccta cgtggaggct gaggcgcgcc
     5461 gggccggact cgccgaatgg caaatgcagc agctactcgc cgcggctggc gaggtcgcgc
     5521 acaatgcctt cctccacggc agtgcccccg ttttcgtgac cgcgtgggag gaacacgaga
     5581 gcttcgtctg ccagatcgag gacgacggcg ggggcatcgt tgatctcgcc gccggctaca
     5641 tgcctccgac gaacgggaac gacggctggg ggctgtggct ggcgcgccag ctgacggact
     5701 cgctcgagat tggacgcggg ccttacggca gcgccgtacg gctcacgatg cacccctccc
     5761 gccccaaacc gccgagggga acactgccga cccggctctg atcctctttc gggaggggcc
     5821 ttcctctcct ggcgaggttg aggttcccgg cggccggggt acaagacggc gaaggctgaa
     5881 cgccttgctc cagcgtgtcg tccaaccagg gaggattctc aatggccgcc gccgatcaac
     5941 ccgtttgccc gcgacatgga cggcccgcag tcgcgtcgag agtgcggctt cgccctgacg
     6001 ggacgcagga ggtcgagtac ctctgcgaga tcgatctcgc cgaagagcag atgtcgagcc
     6061 gcttcggcgg acgcggtggc agtctgttcg acgacttctt ctcggacttc ttcggagacg
     6121 gacaatcgtc ccccggcggc ggcgcgcgca ggccggcggc cgcacagcgg caggtcgagc
     6181 gcgtcgacgt cacgcagttc ttcagcgacg cgacgcgcga gctgttgcag cgggcagcgc
     6241 agacggcggt cgaatgggga agcctcgacc tcgacagcga gcatctcctg tacgcggccc
     6301 tgcaagacga cgtcgttcgc cacgtgctgc gacagatcga cgccgatgcg gaagcgattg
     6361 cggccgagat cgaggacgag gccgacaagg tttcgcgcac cgacgtgtca ccgtcgcttt
     6421 cgccggacgc gaaggccgcg ttgctcgccg cgtatgaaga atcgcgtgag ctcggttctt
     6481 cctacgtcgg ccccgagcat gtgctgctcg cgctcgcacg cgacgacgag agcgaggccg
     6541 gtcagcttct gcagcgcttc ggtgtctcgc acacgaagct gcgcggcgcg gtcatccgcg
     6601 gcgtcgaggc cggcggtggg acgcgcgaga cgagcaagac tccgacgctc gaccagtacg
     6661 gccgtgacct gacgcagatg gcgcgcgagg gcaagctcga tccggtcatc ggacgcgccg
     6721 acgagatcga gcagacgatc gagatcctgt cgcggcggac gaagaacaat cccgcgctga
     6781 tcggcgatcc cggcgtcggc aagaccgcga tcgtcgaggg catcgcccag cgcatcgtca
     6841 acgacgaggt tccggaaacg ctcaccgaca gacgtgtcgt tcaactcgac ctcgccggca
     6901 tggtcgccgg gtcgaagtac cgcggggagt tcgaagagcg gctgaagaag gtgatcgacg
     6961 agatcgtcga gaacgaaggg aacatcatcc tcttcgtcga cgagctgcac acgctcgtcg
     7021 gcgccggcgc ggccgaaggc gcgatggacg cgggcaacat gctcaagccc gcactcgcgc
     7081 gcggggaaat gcacgtggtc ggcgcgacga cgctggacga gtatcgcaag gacgtcgagg
     7141 gcgatcccgc tttggaacgg cgcttccagc ccgtgttcgt gcgcgaaccg accgtcgacg
     7201 agacgatcga gatattgcac gggcggaagg accgcttcga ggcattccac cgcgtccgta
     7261 tctccaacga ggcgatcgtc gccgccgcgg agctctccga ccgctacatc cgcgaccgtt
     7321 tcctacccga caaggcgatc gacctcatcg accaggcgag tgcgcgcgtg cggctgcgca
     7381 acaggaccaa agatgacgac acgaggaacc tcgaggagga tctgaggcgc ctcgcgcggg
     7441 agcgcgacca ggcgacgacg gcggaggact atgaccgcgc cggccagctc accgaccaga
     7501 tggagtcgcg ccgcggcgaa ctggacgagc gtcagaagag gcggcagcgc gcgcccgagg
     7561 tcactgctga ggacgtcgct gtcgtggtgt cacgggcgac cggcatcccc atctcgcagc
     7621 tgacgaccga ggaacggcaa cgcctactcg acctggagca acagctgcac ggccgaatcg
     7681 tgggacagga ggaggccgtg aaagcagtcg cggaagcggt gcgacgatcg cgcgccggcc
     7741 tcggcgatcc gcgcaagccg attggcagct tcctcttcct cggcccgacc ggcgtcggca
     7801 agacagagct cgcgcgcaca ctcgccgacg cgctgttcgg cagcgaggac ctgatggtga
     7861 gattcgacat gagcgagttc caggagcgcc acacggtctc gcggctcgta ggggcgcctc
     7921 cgggctacgt cgggtacgag gaggccggac agctcaccga acaagtgcgg cgccgcccct
     7981 actcggtgct gctcttcgac gagatcgaga aggcgcatcc ggacgtgttc aacatcctgc
     8041 tgcagattct cgacgatggg cgcctcaccg acgcgcaggg gcgcacggtg gatttcaaga
     8101 acaccgtggt gatcatgacg tccaatctcg gcgccgaccg catccagcag caggcgcgta
     8161 cgaacgagcc gttcgaacag ctgaaggctg acctcatgca gatcctccgc cagagcttcc
     8221 ggccggagtt catcaacagg atcgacgaga tcatcgtctt ccgcgcgctg accgaagagc
     8281 agctcgtgga catcacgcag cttctcctcg accggctcga gaggcgcctg cgggcgcagc
     8341 acatcgagct cgagttcacg caagaagcag tgcggctgct cgccaaggag ggctacgacc
     8401 cggagttcgg ggcaaggcct ctggcccgca cgatccagcg gctcgtcgaa aacgagctct
     8461 cgcgcatggt gctgagcggc gaggtcgagc ccggcgacaa ggtgcaggtc gaggccgacg
     8521 gcgacgagct gaagttcgac gtcgagaggg gtgccgcttc ccaggaggtg accgcttggt
     8581 ccgaggagtc ggaggcagca cagacaccgg agcccgcagc cgcccggtaa cgccttccct
     8641 actcgtacac gcccgacgct gatgacatgc tcgcgtcatc gcgaagcgag tcctcgatct
     8701 gatcgtcgca gcggcactcg tcgcgctgct ttctccgctc atcctgattc tgctcgtcgc
     8761 gctggcggcc gacatgatcg tctcgcggcg tgaccgcggc ggcttcttct atcgcgagcc
     8821 ccgcatctcg cgtggccgtc tgttcgggct gctcaagttc cgcacgctca gacgagacgt
     8881 gctggacgag ctgcgcgccg ccggcgggca cgcgcggctc tacgaggcgg acccggcgaa
     8941 tctgacctgg gtcggcaagc gactgctcaa gccctggtac ctggacgagc tgccgcagct
     9001 cttgaatgtc ctgcgcgggg acatcagcct cgtcgggccg cgaccctggc cgcctgagat
     9061 ggtgcgacga caggtcgccg agggcctgga ctaccggaat cggatgctcg cgggcctgac
     9121 cggtccggcc caggtgacaa agggcgaggg gatcgcgtac gaggacctcg actcccggta
     9181 cctccagtgc cagagcacgc tctcgagctg ggcgctggtg cgttacgacc tgcgaatcct
     9241 ctggagaacc gcggcggtca tcgcccgtgg agaaggactg aactattgag ccctcg
//
LOCUS       DASVEU010000234         5200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1136570,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000234 DASVEU010000000
VERSION     DASVEU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136570"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF225_10090"
     CDS             complement(<1..863)
                     /locus_tag="VF225_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7145643.1"
                     /translation="MDPQRRTALVSVVAAILLIALKLGAGLASHSLGLISEALHSGTD
                     LVAALLTFFAVGVAVRPADVSHQYGHGKAEHLSALAEAAILVLASFFIAWRAITHLVG
                     GRNASVEARWYAFAVIGIVIVVDISRTVILWRASRRYRSAALASNALHFASDLGGSVA
                     VLVGLVLVREGHKGADSAAALFVAVLVLLAAARLMKRNVDVLMDRAPADAEQAARAAL
                     ALVEPPVTLKRLRMRQAGPRQFMDVVITAPPGAAVGQGHAAADRVEDALNAALPGSDV
                     VVHVEPQEDEAA"
     gene            complement(932..1525)
                     /locus_tag="VF225_10095"
     CDS             complement(932..1525)
                     /locus_tag="VF225_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145644.1"
                     /translation="MIILFVLLVGVLALAASGLADPGGKGKAKKNGQNRFSATVVVPD
                     SGTCQNVWATDTSNRKWTVKKNNDGTFRVGRQDKGTFVTIAGQSPGACDTTGKHGTHV
                     NVGINGKFHGYLRGTVSNGTFNPTATCAAACIGDTAVFISTFFGAGAKFTCSLGYAGC
                     KFNFQYSSPDKTLKFHHWQDRGTNGVDEHFVGDIANS"
     gene            complement(1641..2747)
                     /gene="hppD"
                     /locus_tag="VF225_10100"
     CDS             complement(1641..2747)
                     /gene="hppD"
                     /locus_tag="VF225_10100"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX7145645.1"
                     /translation="MGQNADFMPIDGWDHLELYVGNAKQAAFYYETAFGFTKTAYAGP
                     ETGVRDRSSYLLEQGDIRFVLTSGLRGDSAINRFACVHGDGVKDTALTVPDAGEAYRQ
                     AVQRGARGVVEPHWVEDDFGRVELATIATYGENVHTFVDRKDYNGPFLPGFQEVPRTN
                     GRPTTGVGLKAIDHVVGNVELGRMDYWVEFYERTMGFTNIIHFADEQIHTEYSALMSK
                     VMADGAGKIKFPINEPAEGKRKSQIEEYLEYFGGPGVQHVALQSEDIVRTVETLEDQG
                     IVFLSTPDAYYEDVGDRVGEIGEDWDDLRRLRILADRDEDGYLLQIFTKTAQDRPTLF
                     FEVIERHGARGFGEGNFKALFEAIEREQALRGNL"
     gene            complement(2757..3644)
                     /locus_tag="VF225_10105"
     CDS             complement(2757..3644)
                     /locus_tag="VF225_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7145646.1"
                     /translation="MRRPNADLMLLATVCLWALNFTVSKYILDHGLHPLAYSSIRYAC
                     AGFIFASITLAWEGSLRIARRDLPIVVFCTAVLFGNQLSFVYALRFTTATTVALIFGT
                     LPIFTALFARGSGIEHLPLRFWIAAGLSFAGVALVALGSGGNVSSNLKGDLLALSGAA
                     TWAAYSVAIGPLLRRYSPFRLSAVFLLAAAVALFVAGSRQLADQSFHFGALVWIGFAF
                     AVLGPLVITNLLWFTAIDRLGPSRASLFANLQPFLAALVALALLSEPITTVQIVGGAA
                     IAGGIVLAPRQRPVVVAAE"
     gene            3719..>5200
                     /locus_tag="VF225_10110"
     CDS             3719..>5200
                     /locus_tag="VF225_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145647.1"
                     /translation="MPLRTVCWLLLAVVAGSTIGFKLALPGSDSAPVPAASGTPYAAP
                     SPLAFAPPNGRPVTPVGLDTGRPSSPGLQHLSGTAAQAGATYSSRSTAAGAPDVSFNS
                     PYVSDLRFLALPGVATTPPPGESPSDTTATTTTPAAPTGPPPEIENVHTLSLTPFSAT
                     IAWRTNVPATSRIAYGLDTPVLWTAPSVATTEHQAALTGLTFSSSYKVAVTAVNENGP
                     GSVDEYLLTTPALSGPVQATTSSGVILLNGQPSFPKMVWAQCPDAVASNLDVGIDLFM
                     GNGCGTGADLAKWVAGRAFVLADAQAPAAARAGTVGTHLPDEWDTHLPGDLTTADALR
                     LVPETPGSGPRFLTLTNHFYSRANPLPQGRGMYPALAASADVLGFDLYPLQNWCRWDS
                     FGDVFDSQLDLVALGRGRPTFQWIEARQMDCMGAQLDPTPETVRAEAWLSIAGGAHAI
                     GYFPNNWSVDVGAEIGRTNHEIQSLVPALVEPPIATSVALGSPV"
BASE COUNT          895 a         1822 c         1657 g          826 t
ORIGIN      
        1 gctgcctcat cctcctgcgg ctccacatga acgacgacgt cgcttccagg caaggccgcg
       61 ttgagcgcat cctccacccg gtctgccgct gcatgaccct gccccacggc agcgcccggc
      121 ggggccgtta tgaccacgtc catgaactgc cgcggtcctg cctgccgcat gcggagccgc
      181 ttcagcgtca ccggcggctc gaccagagca agcgccgcgc gggccgcctg ctctgcatcg
      241 gccggcgccc gatccatcag gacatcgacg tttcgcttca tcagccgtgc ggcagcaagc
      301 agcacgagaa cggcgacgaa cagcgcggct gccgagtccg cgcccttgtg accctcgcgg
      361 acgagcacga gcccgacgag caccgcgaca gatccgccga gatcgctggc gaagtgaagg
      421 gcgttcgacg caagcgccgc gcttcggtag cggcgtgacg ctcgccaaag gatgaccgtg
      481 cgactgatgt cgaccacgat cacgatcccg atgaccgcga acgcgtacca cctcgcctcc
      541 acggacgcgt tgcgcccgcc gacgagatga gtgatcgccc gccaggcgat gaagaagctt
      601 gccaagacca ggatcgccgc ctcggccagg gccgagagat gctctgcctt gccgtggccg
      661 tactggtgcg agacgtcggc gggcctcacg gcgacaccca ccgcgaagaa ggtcagcagc
      721 gccgcgacga ggtcagtgcc ggagtgcaac gcctccgaga tgaggcctag gctgtgcgag
      781 gcgaggcccg cgccgagttt gagcgcgatg agcaggatgg ccgcgaccac cgagacgagc
      841 gccgttcgtc gctgaggatc cacccggcta gtgaaacaga agagcccgcc ggtgaggcgg
      901 gctcttctac gactccttct ctctcctgaa gctaggagtt ggcgatgtcg ccgacgaagt
      961 gctcgtcgac tccgttcgtc cctcgatcct gccagtgatg gaacttcagc gtcttgtccg
     1021 gcgagctgta ctggaagttg aacttgcagc cggcgtagcc gagcgagcac gtgaacttcg
     1081 cacccgcgcc gaagaacgtc gagatgaaga cggcggtgtc cccgatgcac gcggcagcac
     1141 acgtcgcggt cggattgaac gttccgttcg agaccgtgcc cctcaggtac ccgtggaact
     1201 tgccgttgat gccgacgttc acgtgggtgc cgtgcttacc ggtcgtgtcg caggcgccgg
     1261 ggctctgccc ggcgatcgtc acgaacgtgc ccttgtcctg gcggccgaca cggaaggtgc
     1321 cgtcgttgtt cttcttgacg gtccacttgc ggttactggt atccgttgcc cagacgttct
     1381 ggcaggtgcc gctatccggc acgacgaccg tggcgctaaa gcgattctgg ccgttcttct
     1441 tggccttgcc ttttccgccc ggatcggcga ggccggacgc agcaagcgcc aggaccccga
     1501 cgagcaggac gaacaaaatg atcacgcggc gcatctgttt ccaccccttt gctgggattt
     1561 tgtgtccgtg gactagatgc cggaagaccg gaaggcttgc gctctgccgg aaggggaacc
     1621 taggtagcgc tgagggtcac tcagagattg ccgcggaggg cctgctcgcg ttcgatcgcc
     1681 tcgaacagcg ccttgaagtt cccctcgccg aaaccgcgtg cgccgtggcg ttcgatcacc
     1741 tcgaagaaga gcgtcgggcg atcctgtgcg gtcttcgtaa agatctggag gaggtagccg
     1801 tcctcatcgc ggtcggcgag gatccgcagc cggcgcaggt cgtcccagtc ctcgccgatc
     1861 tcgccgacgc ggtcgccgac gtcctcgtag tacgcgtcgg gcgtcgagag gaagacgatc
     1921 ccctgatcct cgagcgtctc gaccgtacga acgatgtcct ccgactgcaa cgcgacgtgt
     1981 tggacgccgg gtcctccgaa atactcgagg tactcctcga tctggctctt ccgctttcct
     2041 tcggccggct cgttgatcgg gaacttgatc tttcccgcgc cgtcggccat caccttcgac
     2101 atcagcgccg agtactcggt gtggatctgc tcgtccgcga aatgaatgat gttcgtgaag
     2161 cccatcgtcc gctcgtagaa ctcgacccag tagtccatcc ggcccaactc gacgttgccg
     2221 acaacgtggt cgatcgcctt caggccgaca ccggtcgtcg gacgtccgtt cgtccgcggc
     2281 acttcctgga agccagggag aaacgggccg ttgtagtcct tgcggtcgac gaaggtgtgg
     2341 acgttctcgc cgtacgtcgc gatcgtcgcc agctcgacgc gcccgaagtc gtcttcgacc
     2401 cagtggggct cgaccacacc tcgcgcccct cgttgcacgg cctgccggta cgcctcccct
     2461 gcgtccggaa cggtcagcgc cgtgtccttg acgccgtctc cgtgcacgca cgcgaagcga
     2521 ttgatcgcgc tgtcgccgcg caaaccgctc gtcaagacga agcggatgtc gccctgctcg
     2581 agcaggtagg acgaacggtc gcgcacgccc gtctccggcc ccgcatacgc cgtcttggtg
     2641 aagccgaacg cggtctcgta gtagaaggcc gcctgcttgg cgttgccgac atagagctcg
     2701 aggtggtccc acccgtcgat gggcatgaag tccgcgttct gacccacgcc cttagcttac
     2761 tcggcagcta cgacgaccgg ccgctgccgc ggggccagca cgatcccgcc cgcgatcgcc
     2821 gcgccgccca cgatctgcac ggtggtgatc ggctccgaca ggagcgcgag cgcgaccaag
     2881 gcggcgagga acggctgcag attggcgaac agcgacgccc gcgatgggcc gaggcggtcg
     2941 atggccgtga accagagcag attcgtgatc acgagcggcc cgagaacggc gaacgcgaag
     3001 ccgatccaca cgagcgcacc gaaatggaag ctctgatccg caagctgccg cgaccccgca
     3061 acgaatagcg caacggcggc ggcgagcagg aagacggcgc tcagcctgaa cggcgaatac
     3121 ctgcgcagga gtggcccgat cgcgacggaa taggcggccc aagtggcggc gccgctcagt
     3181 gccagcaagt cacccttcag gttggacgac acgttcccgc cggacccgag cgcgacgagc
     3241 gcaacccccg cgaaggacag accggcggcg atccagaagc ggagcggcag gtgctcgata
     3301 ccgctcccgc gggcgaagag cgcagtgaag atcggcagcg tcccgaagat gagcgccacc
     3361 gtcgttgcgg tcgtgaagcg cagcgcgtag acgaagctca gctggttgcc aaaaaggacg
     3421 gcggtgcaga agacgacgat gggcaagtcg cggcgcgcga tgcgcagtga cccctcccag
     3481 gcaagcgtga tgctggcgaa gatgaagcct gcgcacgcat agcggatcga cgagtaggcg
     3541 agcgggtgca acccgtggtc gaggatgtac ttcgagacgg tgaagttgag cgcccagaga
     3601 cagaccgtcg cgagcagcat cagatcggcg ttcgggcggc gcacggaggc cgcacgctac
     3661 cgcctcatat ccctcaattg agggagaggt gcggccgcag aaagccgact cctagagagt
     3721 gcccctgcgc accgtctgtt ggctcctgct tgccgtcgtt gccggttcga cgatcggctt
     3781 caagcttgct ctgcccggct ccgactcagc cccggtcccg gccgcgtcgg gcacccccta
     3841 tgcagcgccg tcaccgctcg ccttcgctcc cccgaacgga cggccggtga ccccggttgg
     3901 cctcgacacc ggccggccgt caagccccgg cctgcagcat ctctccggaa ccgccgcaca
     3961 agccggcgca acctacagct cgcgatccac cgcagccggc gcgccagacg tctctttcaa
     4021 ctcgccgtac gtcagcgatc tgaggttcct cgccctcccg ggcgtcgcga ccactccgcc
     4081 gccaggcgag tcgcccagcg acacgacggc gaccaccacg acgcccgccg cgcccaccgg
     4141 tcctccgccg gagatcgaga acgtgcacac gctctccctc accccgttct ccgcgaccat
     4201 cgcctggcgg acgaacgtgc ctgcgacgtc gcgcatcgcg tacgggctcg acacgccggt
     4261 cctctggacc gcaccgagcg tcgcgaccac cgaacaccag gcggccctca ccggcctgac
     4321 tttctcgagc tcctacaagg tggccgtcac agccgtcaac gaaaacggcc ccggaagtgt
     4381 cgacgagtac ctcctcacca ctcccgctct ctccggtccc gtgcaagcga cgacttccag
     4441 cggggtcatc ctgctcaacg ggcagccgtc gttcccgaag atggtctggg cccagtgtcc
     4501 cgacgccgtc gcgtcgaatc tcgacgtcgg catcgacctc ttcatgggca acggctgcgg
     4561 caccggggcc gaccttgcga agtgggtcgc cggcagagcg ttcgtgctcg ccgacgcaca
     4621 ggcgcccgca gccgcgcgcg ccggcaccgt cgggacgcac ctacccgacg aatgggacac
     4681 gcatcttccc ggcgacctga cgactgcgga cgcgctgcgg ttggtcccgg agaccccggg
     4741 ctcgggcccg cggttcctca cgctgaccaa tcacttctac agccgcgcca acccgctccc
     4801 gcaggggaga gggatgtacc cggctctggc cgcgagcgcc gacgtgctcg ggttcgatct
     4861 ctaccccttg cagaactggt gccgctggga cagcttcggt gacgtcttcg attcgcagct
     4921 cgacctcgtc gccctcggac gcggccgccc cacgttccag tggatcgaag cgcgccagat
     4981 ggactgcatg ggcgcgcaac tcgatccgac tcccgagacc gttcgcgcgg aggcctggct
     5041 atcgatcgcc ggcggcgcgc atgcgatcgg ttacttcccg aacaactgga gtgtcgacgt
     5101 gggcgcggag atcggccgca cgaatcacga gatccagtca ctcgtccctg ccctcgtcga
     5161 gcccccgatc gcaacgagcg tcgcgctcgg cagccccgtg
//
LOCUS       DASVEU010000235         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1720783,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000235 DASVEU010000000
VERSION     DASVEU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720783"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VF225_10115"
     CDS             <1..1049
                     /locus_tag="VF225_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145648.1"
                     /translation="MATLLPVGTRKGLFLLRSDEDRKEWELEGPLLPGWPVYHAIVDP
                     RDGVLYAATNSFIYGGTVHRSADLGRNWERAEEIGLPEESGLKLNATWHVEPGKDSEL
                     WLGGDPGVLFRSDDGGVTWEVNRGLLEHPTREKWQPGAGGMCCHSIQVVDDTIYIAIS
                     AAGAFRSDDGGDTWAPINKEVAADFHPEKYPEVGQCVHKLLVHPARPDRLWQQNHCGV
                     YRSDDRGDNWERLDGNGLPSDFGFGLALDAEDPDVAYVIPEVSGEQHFTPDGRLGVYR
                     TGDAGASWQLTANGLPDRAWSAVLREGFAYDEGGLYFGTQSGSVWTAPRGGGEWIEAV
                     RDLPPILSVEAAHV"
     gene            1060..1308
                     /locus_tag="VF225_10120"
     CDS             1060..1308
                     /locus_tag="VF225_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7145649.1"
                     /translation="MLLPGTLAAEAGGQKRFELRAATVGEALRQLPVANLLFDEAGEL
                     RRLVNVYVDGVDARGRRLLDEPLTEESEVRIVGAVAGG"
     gene            1433..2020
                     /locus_tag="VF225_10125"
     CDS             1433..2020
                     /locus_tag="VF225_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145650.1"
                     /translation="MRAIAVLLVVVGGLAALGFARHEERVREQDQLATIASDLAGRRV
                     GVRCPSFAASLVDTRGEAGRVQFDELGRPANHTDLSPQTCKALRHLNAVDFTCIERGE
                     CGFKEFNAGWAAHTLAHESFHLRGFQDEGVTECYALQNTSFVAKRLGVPKQQAQDLQA
                     WLYKRGYPNEPTEYHSQQCYAGGPLDLRPQQTQFP"
     gene            complement(2021..3052)
                     /locus_tag="VF225_10130"
     CDS             complement(2021..3052)
                     /locus_tag="VF225_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7145651.1"
                     /translation="MNRSLALLGVLVLLVAAGCGGGGKKSSASTTTTGSTAQKIKIGL
                     VTDIGGLNDRGFNHLSYVGLLRAQGELGIAQRVYQAKSTQEYVPNLSTFARDGYDLTI
                     GVGFTEADAIDTVATNFPKSKFAIVDVDQTEESHKPANLLGLLFKEQETGYLVGYLAG
                     LEEKRKPGKDVIGSVGGQKQPPVDRFIAGYQAGAKAADPGITTLNNYSEDFADLAKCK
                     QIALNQIGQGAGVIFQVAGGCGLGALDAAKEKGVWGIGVDADQSFLGPYILTSAVKRV
                     DTAVFDAIKLVVDGKFKGGNVVFGLKDNGVAIGKISPKVPKSEVAKVMQIRADIIAGK
                     IKNIPTIVK"
     gene            3135..3809
                     /locus_tag="VF225_10135"
     CDS             3135..3809
                     /locus_tag="VF225_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7145652.1"
                     /translation="MTLGVVLQVRDGVLQVLLWQRARQPFKGLWSVPGGYLAEHDTLE
                     ESIRRHLAEKVDVRELSHLEQLETLSDPKRSPREWQLATAYLGLVPSDIDPELPGDTD
                     WHPVDDLPPLAFDHGSIVLVGRERLRAKLSYTNLGFALAPDEFAISELRDLYAAALGH
                     DVSATNLQRVLLRREVLEPTGRQRASGPTGGRPGSLFRFRTPELEITDQFAVLRPPGP
                     ARLPVA"
     gene            3860..4396
                     /locus_tag="VF225_10140"
     CDS             3860..4396
                     /locus_tag="VF225_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7145653.1"
                     /translation="MAGSRGDERAWIRGAQSGSVPDLEALFRHHWPRAYRAAYLVVHD
                     SAAAEDIAQEAFLAAVRGLDRFDRRRPFGPWLHRIVVNRAIDWSRSRALRRESGDAWL
                     GLVAAPAPPDGEHAQTLAAALADLSPEHRAVIVMRHLLEYTPGEIAEALGLPRGTVNS
                     RLRRGLDELADLLDEDLQ"
     gene            4393..5508
                     /locus_tag="VF225_10145"
     CDS             4393..5508
                     /locus_tag="VF225_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145654.1"
                     /translation="MRRWDAPAPDERVAGDRSWEVVREAYEERIRVPRKRDWRPVAIV
                     AVAAAVVAAAVTPPGHAVLGSLRDAVRGERNASPALVSLPTPHSRLLVKSAAGTWVVQ
                     SDGSKRLLRGYHDASWSPHGIYIAAVRGHELRALEPNGAVHWSIGRKGTIRAPRWSFD
                     GFRIAYFAGPTLRVINGDGTQDRVLTRDARAGVSAWQPASHVLAYVNAAGNIVVRDAD
                     RPTRLAVIRTRLAPRQLQWTTNGRLLVAVGSHTIGIFAPRGPQLRRIDTGAATIAASS
                     VSPDGKSVAFIEAGPDQSSLQITGTGAGPTREIFKGVGIFSNVVWSPDGRVLLLDWSS
                     ARQWLFIHSVPVKRIAAVSNIRANFGKEPSLMGWCCP"
     gene            5698..>6141
                     /gene="rsgA"
                     /locus_tag="VF225_10150"
     CDS             5698..>6141
                     /gene="rsgA"
                     /locus_tag="VF225_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HEX7145655.1"
                     /translation="MPALDALGWDAELASAFEQLQDDNLFPARVAAQHRGEFVLFAEE
                     SELRARAAGRLFYGHEVGGQLPAVGDWVGVTPPATITSILPRRGAFIRKNAGHDSTEQ
                     VLAANVDAAFLLAGLDDDFSLRRLERYITIAWESGAQPIVVLTKAD"
BASE COUNT          980 a         2093 c         2040 g         1028 t
ORIGIN      
        1 cgatggccac gctcttaccc gtcggcacca ggaaggggct cttcctcctc cgcagcgacg
       61 aggaccgcaa ggagtgggag ctcgagggcc cgctgctgcc cggctggccg gtgtaccacg
      121 cgatcgtcga tccgcgtgac ggcgtcctgt acgcggcgac gaacagcttc atctacggcg
      181 ggacggtgca ccgctcggcc gatctcggcc ggaactggga gcgcgcggag gagatcggcc
      241 tgccggagga atcgggcctc aagctcaacg cgacctggca cgtcgagcca ggcaaagact
      301 cggagctgtg gctcggcggc gaccccggcg tgctgtttcg atccgacgac ggcggcgtca
      361 cctgggaagt gaaccgtggg ctcctcgagc acccgacgcg ggagaagtgg cagccgggcg
      421 ccggcggcat gtgctgccac tcgatccagg tcgtcgacga cacgatctac atcgccatct
      481 ccgcagcggg cgcgttccgc agcgacgacg gcggcgacac gtgggcaccg atcaacaagg
      541 aggtcgcggc cgacttccat ccggagaagt acccggaggt cgggcaatgc gtgcacaagc
      601 tgctcgtgca tccggcgcga ccggaccggc tgtggcagca gaatcactgc ggcgtctatc
      661 gctccgacga ccgtggagac aactgggaac gactcgacgg caacggcctc ccgtcggact
      721 tcgggttcgg gctcgcgctg gacgcggagg acccggacgt cgcctacgtg atcccggaag
      781 tgtcgggcga gcagcacttc acgcccgacg ggcggctcgg tgtctaccgg accggcgacg
      841 ccggagcttc atggcagctc accgcgaacg ggcttcccga ccgcgcgtgg tcagcggtgc
      901 tgcgagaggg cttcgcctac gacgagggcg ggctgtactt cggaacacag agcggctccg
      961 tctggactgc cccccgcggc ggcggtgagt ggatcgaagc ggtgcgggat ctgccgccga
     1021 tcctctcggt cgaagccgcg cacgtctgac ttggcggtcg tgctcctgcc gggaacgctc
     1081 gccgcggagg cgggcgggca gaagcgcttc gagctgagag ccgcgaccgt cggggaggcc
     1141 ttgcgccagc tcccggtcgc gaatctcctg ttcgacgagg ccggggagct gcgtcggctc
     1201 gtcaacgtct acgtcgacgg cgtggacgca cgcggccgcc ggctgctgga cgagccgctg
     1261 accgaggagt cggaggttcg gatcgtcggc gcggtggccg gcggctgact cgcaggcttg
     1321 tggctctgag ccacaagtcg cgattccgcc accgctgaat cgagacgact tagtggctct
     1381 gagccactaa gtcggaatca agactgtccg aggcgctgcc gatccttacc cggtgcgggc
     1441 catcgcggta ctcctcgtcg ttgtcggcgg cctcgcggcg ctcggcttcg cgcgccacga
     1501 ggagcgcgta cgcgagcagg atcaactggc cacgatcgcc agcgacctcg ccggccgcag
     1561 agtgggcgtc cgctgcccaa gcttcgccgc gagcctggtc gacacgcggg gcgaggcggg
     1621 acgggtgcag ttcgacgagc tgggccgtcc ggccaaccac acggatctct cgccgcagac
     1681 gtgcaaggca ttgcgccatc tgaatgctgt cgacttcacg tgcatcgaac gcggcgagtg
     1741 cgggttcaag gaattcaacg ccggctgggc cgcacacaca ctcgcccacg agtcgttcca
     1801 cctgcgcggc ttccaggacg agggtgtgac cgagtgctac gcactgcaga acaccagctt
     1861 cgtggcgaag cgactcggcg tgccgaaaca gcaggcgcag gatctccagg cgtggttgta
     1921 caagcgcggc tatccgaacg agccgacgga gtaccactcc cagcagtgtt acgccggagg
     1981 cccgttggac ctccgacctc agcagactca gttcccttag ttacttcacg atagtcggga
     2041 tgttcttgat cttgccggcg atgatgtcgg cgcggatctg catcaccttc gcgacctcgg
     2101 acttcggcac cttcgggctg atcttgccga tggcgacacc gttgtccttg aggccgaaga
     2161 cgacgttccc acccttgaac ttcccgtcga cgacaagctt gatcgcatcg aacaccgccg
     2221 tatcgacgcg cttcaccgcg ctggtcagga tgtacggtcc gaggaaggac tggtcggcgt
     2281 cgacgccgat gccccagaca cccttctcct tcgccgcgtc gagcgcgccg agaccacagc
     2341 cgccggcgac ctggaagatc acgcccgcgc cctgcccgat ctggttcagc gcgatctgct
     2401 tgcacttcgc aagatccgcg aagtcctcgg aataattgtt cagcgtggtg atcccgggat
     2461 ccgcagcctt ggctcccgcc tggtagccgg cgatgaagcg atccacgggc ggctgcttct
     2521 ggccgccgac cgaaccgatc acatccttgc ccggcttgcg cttctcctcg aggcccgcga
     2581 gatacccgac caggtaaccg gtctcctgct ccttgaagag caggcccagc agattcgcgg
     2641 gcttgtggct ttcctcggtc tggtcgacgt cgacgatcgc gaacttcgac ttcgggaagt
     2701 tcgtcgccac ggtgtcgatc gcgtccgcct cggtgaagcc gacgccgatg gtcaggtcat
     2761 agccgtctcg ggcgaacgtc gacaggttcg ggacgtactc ctgcgtcgac ttcgcctggt
     2821 agacgcgctg cgcgatgccc agctcgccct gcgctctgag gagcccgaca tacgagaggt
     2881 ggttgaagcc gcggtcgttg agcccaccga tgtcggtgac caggccgatc ttgatcttct
     2941 gggcggtgct cccggtggtc gtcgtgctgg cggaggactt cttgccaccg ccgccacagc
     3001 cggcagcgac gagcagcacg aggacgccga ggagtgcgag cgatcgattc atccctgacc
     3061 tttccgctaa ggtttgcgac tatggggcga aaactgtgag cgagcctacc agcgggcacc
     3121 ctactcccgc tcatgtgacc ctcggggtcg ttctccaggt gcgagacggc gtgttgcagg
     3181 tgctcctctg gcagcgcgcg cggcaaccgt tcaaggggct gtggtcagtt ccggggggct
     3241 atctcgcaga gcacgacacg ctcgaggagt cgatccgccg ccacctcgcc gaaaaggtcg
     3301 acgtacgcga gctgtcgcat ctcgagcagc tcgagacgct gagcgatccg aagcgctcgc
     3361 cgcgcgagtg gcagctcgcg accgcttacc tcggccttgt cccgagcgac atcgatcccg
     3421 agcttcccgg cgacaccgac tggcatccgg tcgatgacct ccccccactc gcgttcgacc
     3481 acgggtcgat cgtgctggtc ggacgcgagc ggctgcgcgc gaagttgtcg tacacgaacc
     3541 ttggttttgc gctcgcgccc gacgagttcg cgatctcgga gctgcgcgat ctgtacgcgg
     3601 ccgctcttgg acacgacgtc tccgcgacga acctccagcg tgtgctgcta cggcgtgagg
     3661 tgctcgagcc gacgggccga caacgcgcgt ccggtcccac cggcggtcgg cccggctcgc
     3721 tcttccgctt ccggacgccc gagctcgaga tcacggacca gttcgccgtg ctccgcccgc
     3781 cgggacctgc acgccttcct gtcgcgtagt tctcccgcat ggacctgagc ggactcccag
     3841 ctcgtagagt cggtgcgcga tggcgggctc tcgaggcgac gagcgcgcct ggattcgggg
     3901 cgcacagtcg gggtcggtcc ccgatctcga agcgctgttc cgacatcact ggccccgtgc
     3961 ctaccgcgcc gcgtatctcg tcgtgcacga ctccgccgct gcggaggaca tcgcgcagga
     4021 ggcgttcctg gccgcggtgc gcgggctcga ccgcttcgac cgccgcaggc cgttcggccc
     4081 atggctgcac cgcatcgtcg tcaaccgcgc catcgactgg tcccggtcgc gcgcgttgcg
     4141 ccgtgagtcg ggcgacgcct ggcttggcct cgtcgccgct cccgctcctc cggacggcga
     4201 gcacgcgcaa acgctcgcag ctgcactcgc cgacctttca cctgaacatc gcgccgtgat
     4261 cgtcatgcga catctcctgg agtacacacc cggcgaaatc gcggaagcct tgggactccc
     4321 acggggcacg gtcaactcgc ggctacgccg cggcctggac gagctcgccg acctgctcga
     4381 tgaggacttg cagtgagacg ctgggacgcc cccgcaccgg acgagcgagt agccggcgac
     4441 cgcagctggg aggtcgtgcg cgaagcctac gaggagcgca tcagggtgcc gcgcaaacgc
     4501 gactggcggc cggttgcgat cgtcgccgtc gcggccgcag tggttgccgc cgcggtgact
     4561 ccacctgggc acgcggttct cggctccctg cgcgacgcgg tgcgaggcga aaggaatgcg
     4621 tctccggcac tcgtttccct cccgaccccc cactcccgcc tgctcgtgaa gtcggcggcg
     4681 ggaacatggg tcgtgcagag cgatggctcg aagcgtctgt tgcggggtta tcacgacgcc
     4741 tcctggtcac cacacgggat ctacatcgcg gcggttcgcg gacatgagct gcgagcgctc
     4801 gagcccaacg gcgccgtcca ctggtcaatc ggccgaaagg gcacgatccg ggctccgcgc
     4861 tggtccttcg acggctttcg cattgcgtac tttgccggcc cgacgttgcg ggtgatcaac
     4921 ggcgacggga cgcaggatcg cgttctgacg cgcgacgcgc gcgcgggagt gtcagcttgg
     4981 cagccggcgt cacacgtcct ggcctacgta aacgcagccg ggaacatcgt cgtacgcgat
     5041 gccgaccgac ccacgcgtct cgcagtgatc cgcacgcggc tcgctccgcg acaactgcaa
     5101 tggacgacaa acggacggct gctcgtagcg gttggatcgc acacgatcgg catcttcgca
     5161 ccgcgcgggc cgcagctacg acgaatcgac accggtgctg cgacgattgc tgcgtcatcg
     5221 gtctcgccgg acggcaagag cgtcgccttc atcgaagcgg ggccagatca gagctcgctg
     5281 cagatcacgg ggactggggc cggccccacc cgcgagatct tcaagggcgt ggggatcttc
     5341 agcaacgtcg tctggtcccc ggacggtcgc gtgctcctac tggactggag cagtgcccgg
     5401 cagtggctct tcatccactc agtacccgtg aagagaatcg ctgcggtctc gaacatccgc
     5461 gccaacttcg gcaaggaacc ctcgctcatg gggtggtgct gtccgtaacc gcgtgctacc
     5521 gtcagtgcgt tcccggcgat aaggaggtcg aacaaatgac gactgcgctt gcctccttcc
     5581 gaagagccac gcggctcgtc gtctagccgg caccactttc acacctagcg cgagtccgtg
     5641 agctgatccg cgtaacgcgg atctctgtcg ttcactcact cacaaaggag actcgctttg
     5701 cctgctctgg atgcgctcgg atgggacgcc gaactggcgt ccgcgttcga gcaactgcaa
     5761 gacgacaatc tatttccggc ccgcgtcgcc gcgcagcacc gcggcgagtt cgtgctcttc
     5821 gccgaggaga gcgagctacg agcgcgcgca gccgggcgcc tcttctacgg tcacgaggtc
     5881 ggcgggcagc tgcctgctgt cggcgattgg gtcggtgtga cgccaccggc gacgatcacc
     5941 tcgatcctcc cgcggcgagg tgcgttcatc cgcaagaacg caggccacga ctcgaccgag
     6001 caggtgctcg ccgccaacgt cgacgcggcc tttctcctcg ccgggctcga cgacgacttc
     6061 agcctgcgcc ggctggagcg ctacatcacg atcgcgtggg agagcggcgc tcaaccgatc
     6121 gtggtgctca cgaaggccga c
//
LOCUS       DASVEU010000236         6001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_943007,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000236 DASVEU010000000
VERSION     DASVEU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943007"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VF225_10155"
     CDS             complement(<1..1055)
                     /locus_tag="VF225_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7145656.1"
                     /translation="MSSDLSRRLAVARGDEPADVVVRGGRVLSVFTREWLDTDIAIAD
                     GWVAGLGEYEGHEVVDASGRYVVPGFLDAHMHLESTKLLPDEFARLVLPLGTTAVVLD
                     PHELANVLGTDGVHWLLDACEGLPLDFYFMASSSIPASKFESPRRPLLSGDLESLLRR
                     RRVLGLAEMMNFPGVINGDANELDKLRLANGVQVDGHAPGVLGKELNAYVASGIRSDH
                     EALTVEEGRERLRSGMWLLIREASMARNLHALLPLVKEFGTNRIAFCTDDRDPEDIAE
                     NGHINGMVREAVAAGVAPEDAVVLATINPATWHRLWHLGAIAPGYQADLLLLPDLERF
                     EPELTLKAGRPVKEIPEP"
     gene            1095..1877
                     /locus_tag="VF225_10160"
     CDS             1095..1877
                     /locus_tag="VF225_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7145657.1"
                     /translation="MDVLTPRNLEEALRLKAQRPDAVPIQGGTDLMVELNFDRARPVA
                     LLNLNEVAELRGWSRQGGTVWLGSGLTYAEAMEPSLAEALPALAEAARTVGGPQIRNR
                     GTIGGNLGTASPAGDALPPLLVCGAEVKIAGVRGERTVPLPEFLVGPKRNVVAEDELI
                     LGVSVDATESRQTFMKVGPRNAMVIAVVSLAVRVDGDLRAAFGSAGPVATLVRADLGD
                     ADSFPQRVAEAASPIDDVRGTALYRRHALRILTRRALDRCLV"
     gene            1874..2332
                     /locus_tag="VF225_10165"
     CDS             1874..2332
                     /locus_tag="VF225_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7145658.1"
                     /translation="MRIELRVNGEQFEADVWEGESLLYALRERLGFPGSKNACEQGEC
                     GSCSVLLDGKLVCACLVLAAQTEGHEVVTVEGLAENGRLHKVQEAFVSTGAVQCGFCT
                     PGLVVATADLLARNVYPSDDDIREALSGNLCRCTGYAKIFDAVRLAAASL"
     gene            2329..4551
                     /locus_tag="VF225_10170"
     CDS             2329..4551
                     /locus_tag="VF225_10170"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7145659.1"
                     /translation="MSEATAPKLELGRVGESVPRVDGIPKVIGEFAYSSDLQAAGMLW
                     GHTVRSPHAHARIVAIDISGAVGMPGVHAVLTQEDVPGEKRYGLEFQDQPVLAYERVR
                     YFGEAVALVAAEHPEQARRAAERIRVEYEPLEPIVDPEKATEQEPLHPDRPTMGHGYR
                     DDPRPNIVRSLLIRHGDPEADGDVTVSGVYEVGIQDQAFLGPESGLAVPDGEGGVDVY
                     VATQWLHVDRDQVAPCLNLKPEQVRIHLAGVGGAFGGREDLSMQIHGALLALHTNRPV
                     KIVYNREESFVGHIHRHPAKIWAEHRATLDGRLVNVRVRILLDGGAYASSSTAVTSNA
                     ASFAVGPYAVDNALIDSTCVYTNNPPCGAMRGFGAVQVCFAHEAQMDKLADALDIDPI
                     ELRLLNALGPGGSLPTGQKVTGTLPVADVIRRAAALDVPEPEELPRDPMRLPGGSGNT
                     TRGEGVKRGVGFAVGFKNIGYSEGFDDYTAARVQLHADGSATVHCAAAEVGQGVANVI
                     LQVARTELGIRDVVLAPHTTGTVDSAGSASASRMTWMASGAVRDACRAALEERDKKHG
                     GREVDVERIYRHPRTTPLDPDTGQITGERAHVAYACAAMRVVAEVDVELGLTRVVWIG
                     TAQDVGKAVNPQAVRGQIEGGTAQGLGLALMEEIQTRDGLITNASFTDYLIPTTLDMP
                     PVVSELIEEAEPGAPYGVKGVGEPPTVVSTAAVVAALRAATGRELTRVPVRPDDIVGL
                     "
     gene            4749..5027
                     /locus_tag="VF225_10175"
     CDS             4749..5027
                     /locus_tag="VF225_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145660.1"
                     /translation="MVRAIVLYEEEPDAARYEQHVELCRKVPGGTFRHGRVFGAPMGE
                     PPYRYHAEWEWPDLDAFKSAARSEEFMATGKDAMEMGGKFTVMFADVD"
     gene            5020..5835
                     /locus_tag="VF225_10180"
     CDS             5020..5835
                     /locus_tag="VF225_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7145661.1"
                     /translation="MTEPTHPRPSSPDELGFESIVYEKAPPRATITLNRPEILNAFDF
                     RMLRELARACEDASWDDEIRVVVLTGAGRAFCVGADLKSWSADYLGKPKEYWKWFGAF
                     KDAHDRLREIGKPTVARINGIAVGGGNELQMACDLAVMVEDAFIRHVGLEHGSVPAGG
                     ATQWLPIFVGDRRAREIIMLCEEIPAAKAAEWGLINRAVPAAELDAAVDEYVAKLATK
                     LPQTTRYAKQQLNFWRDLSWHETVNHARDWLALSMLGDEAQEAVKAFLKRSSS"
BASE COUNT         1006 a         1970 c         2105 g          920 t
ORIGIN      
        1 ggctccggaa tctccttgac cggacggccg gccttcagcg tcaactcggg ctcgaaccgt
       61 tccaggtcag gcaggagcag gaggtcggcc tggtagcccg gtgcaatcgc acccaggtgc
      121 cagagccggt gccacgtcgc aggattgatc gtagcgagca cgaccgcatc ctcaggtgcg
      181 acgcccgctg cgaccgcctc gcgcaccatc ccgttgatgt ggccgttctc ggcgatgtcc
      241 tccgggtcgc ggtcgtcggt gcagaacgcg atccggttcg tgccgaactc cttcaccagg
      301 ggcaaaagcg cgtgcaagtt ccgtgccatc gacgcctcgc ggatcaggag ccacatgccg
      361 ctgcgcaggc gctcgcggcc ctcctctacg gtcagcgcct cgtgatccga gcggatgccc
      421 gaggcgacgt acgcgttgag ctccttgccg agcacgcccg gcgcgtggcc gtcgacttgc
      481 acgccgttcg ccaggcgcag cttgtccagc tcgttcgcat caccgttgat gacgcccggg
      541 aaattcatca tctcggcgag cccgaggaca cgccggcggc ggagcaggct ctcgaggtct
      601 ccgctcagga gtggacgccg cggcgactcg aacttcgacg cggggatgga cgacgacgcc
      661 atgaagtaga agtcgagcgg taggccttcg cacgcatcga gcagccagtg cacaccatcg
      721 gtgccgagca cgttcgcaag ctcgtgcggg tcgaggacga ccgctgtcgt acccagcggc
      781 agcacgagcc gggcgaactc gtccggtagc aacttcgtcg actccagatg catgtgcgcg
      841 tcgaggaagc ccggaacgac gtaacgcccc gacgcatcga cgacctcgtg tccctcgtac
      901 tcgcccagcc cggcgaccca gccgtccgcg attgcgatgt cggtgtcgag ccactcacgc
      961 gtgaacacgg acaggacgcg gccgccgcgc acgacgacgt cggcgggctc gtcgcctcgg
     1021 gccactgcaa ggcgccggga gaggtcgctg gacacggcga cgattctccc acgacggcga
     1081 agtacgcttg acccgtggac gtgctgacgc cccgaaacct cgaagaagcg ttgcgtctga
     1141 aggcgcagcg ccccgacgcg gtgcctatcc agggcggcac cgacctgatg gtcgaactga
     1201 atttcgaccg cgccagaccg gtggctctcc tcaatctcaa cgaggtcgcc gagctgcggg
     1261 gctggtcgcg ccagggcggg acggtctggc tcggatcagg cctgacctac gcggaagcaa
     1321 tggagccgtc gctcgccgag gcgctgcctg cgctcgccga ggcggcgaga accgtcggcg
     1381 ggccgcaaat caggaaccga ggaacgatcg gcggcaatct cgggacggca tcgcccgcag
     1441 gcgacgcgct gccgccgctg ctcgtgtgcg gcgccgaggt gaagattgcg ggcgttcgcg
     1501 gcgaacgcac ggtccccctg cccgagtttc tcgtcggacc gaagcggaac gtcgtcgccg
     1561 aagacgagct catcctcgga gtgtcggtcg atgccacgga gagccgccag acgttcatga
     1621 aggtcggccc gcgcaacgcg atggtgatcg cggtcgtctc gctcgccgtg cgcgtcgacg
     1681 gtgacctgcg cgcggcattc ggctcggccg gaccggtggc gacgctcgtg cgcgcggatc
     1741 tcggggacgc ggattccttc ccgcagcgcg ttgccgaggc ggcgagcccg atcgacgacg
     1801 ttcgcggcac ggccctgtac aggcggcacg cgctgcgcat cctcacgcgc cgtgcgctgg
     1861 accggtgcct cgtatgagga tcgagctgcg cgtcaacggc gagcaattcg aggcggacgt
     1921 ctgggaaggc gaaagcctcc tctacgcttt gcgcgagcgt cttggcttcc ctgggtcgaa
     1981 gaacgcgtgc gagcaggggg agtgcggctc gtgctcggtg cttctcgacg ggaagcttgt
     2041 ctgcgcgtgc ctcgtgctcg cagcgcagac cgagggtcac gaggtcgtca cggtcgaagg
     2101 gctggccgag aacgggcggt tgcacaaggt gcaggaagcg ttcgtctcga cgggcgcggt
     2161 gcagtgcgga ttctgtacac ctgggctcgt agtggcgacc gccgatctgc tcgcgcgcaa
     2221 cgtctacccg agtgacgacg acatccgcga ggcgctgtcg ggaaatcttt gccgctgcac
     2281 gggttacgcg aagatcttcg atgcggtacg cctggcggcg gcctcgctgt gagcgaagcc
     2341 accgccccaa agctggagct aggacgcgtc ggggagagcg tcccgcgcgt cgacggcatt
     2401 ccgaaggtca tcggggagtt cgcctattcc agcgacctgc aggcggcagg gatgctctgg
     2461 ggccacacgg tgcgcagccc gcacgcacac gcgcggatcg tggcgatcga catctcgggg
     2521 gcggtcggca tgcccggcgt gcacgcggtg ctgacacaag aagacgttcc cggtgagaag
     2581 cgctacgggc tcgagttcca ggaccagccc gtccttgcct acgagcgcgt gcgttacttc
     2641 ggagaagcgg ttgcgctcgt agccgcggaa catccggagc aggcgcgccg cgcggccgag
     2701 cgtattcgcg tcgagtacga gccactcgag ccgatcgtcg acccggaaaa ggcgacggag
     2761 caggagccgc tccatcccga ccgccccacg atgggccacg gctaccggga cgatccgcgc
     2821 ccgaacatcg tgcgctcgct cctcatcagg cacggggatc ccgaggccga cggcgacgtg
     2881 accgtctccg gggtctacga ggtcgggatc caggaccagg cgttcctcgg gccggaatcc
     2941 gggctcgcgg tgccggacgg cgagggcggc gtcgacgtct acgtcgcaac gcagtggctg
     3001 cacgtagacc gcgaccaggt cgcgccgtgc ctgaacctga agcccgagca ggtgcgcatc
     3061 cacctcgcgg gtgtcggcgg cgcattcggc ggccgcgagg acctgtcgat gcagatccac
     3121 ggtgcattgc tcgctctgca cacgaaccgt cccgtgaaga tcgtctacaa ccgcgaggag
     3181 tcgttcgtcg gccacatcca ccgccatccc gcaaagatct gggccgagca ccgcgcgacg
     3241 ctcgacggca ggctggtcaa cgtccgtgtg cggatcctgc tcgacggcgg ggcgtacgcg
     3301 tcaagttcga ctgcggtcac gtccaacgcc gcctcgttcg cagtcgggcc gtacgcggtc
     3361 gacaacgcgc tgatcgacag tacgtgcgtc tatacgaaca acccaccgtg cggtgccatg
     3421 cgtgggttcg gtgcggtcca ggtctgcttc gctcacgagg cgcagatgga caagctcgcc
     3481 gacgcgctcg acatcgaccc gatcgagctg cggctgctga acgcgctcgg gcccggcggc
     3541 tcgttgccga cagggcagaa ggtcaccggg acgctcccgg tcgccgacgt cattcgccgt
     3601 gcggccgcgc tcgacgtccc cgagcccgag gagctcccgc gcgatccgat gcgccttccc
     3661 ggcggctcgg gcaatacgac gcgcggcgag ggagtcaaac gcggcgtcgg cttcgcggtc
     3721 ggcttcaaga acatcggcta ctccgagggt ttcgacgact acaccgcagc gagggtgcaa
     3781 cttcatgccg atggcagcgc caccgtccac tgtgcggctg ccgaggtggg ccagggcgtt
     3841 gcgaacgtca tcctccaggt tgcgcgcacc gagctcggca tacgagacgt cgttcttgca
     3901 ccgcacacga ctgggaccgt cgactccgcg ggctcggcat cggcttcgcg gatgacatgg
     3961 atggcgtcag gcgccgtgcg tgacgcgtgt cgggcggcgt tggaggagcg ggacaagaaa
     4021 cacggcggcc gggaggtcga tgtcgagcgg atctatcgcc atccgcgcac gacgccactc
     4081 gatccggata ccggccagat caccggcgag cgtgcgcacg ttgcgtacgc gtgcgccgcg
     4141 atgcgtgtcg tcgccgaggt cgacgtcgag ctgggactga cgcgcgtcgt gtggatcggc
     4201 accgctcagg atgtcggcaa ggctgtcaat ccccaggccg tgcgggggca gatcgaaggc
     4261 gggacggcac aagggctcgg cctcgcgctg atggaggaga tccagacacg cgacgggctg
     4321 atcacgaacg cgagcttcac cgattacctg atcccgacga cgctcgacat gccgccggtc
     4381 gtctcggaac tgatcgagga agccgagccg ggtgcgccgt acggcgtcaa gggcgtcggc
     4441 gagccgccga cggtcgtctc gacggcggcc gtggtcgccg ccctgcgtgc ggcaacgggc
     4501 cgcgagctca cgcgggtccc ggtgcggccg gacgacatcg tcggtctctg agggcttagt
     4561 ggctcagagc cactaatgcg taatcgagcc ccacgctctg cgcggcgagc caggaaaacc
     4621 gacttagtgg ctcagagcca ctaagtcttg tagagcgaaa gcgctgcgcc ggcacccagt
     4681 aagaccaggc cgagcgtttt cgggatgaag ctatacgctc acgccgtcta cgacgagggg
     4741 gaagaggaat ggttcgcgcg atcgtgctgt acgaggagga accggacgcg gcccgttacg
     4801 agcaacacgt tgagctctgc cgcaaggtgc cgggtggaac gttccgtcac ggacgagtct
     4861 tcggcgcacc catgggagag ccgccctatc gctaccacgc cgagtgggag tggccggacc
     4921 tggatgcgtt caagtcggct gcacgcagcg aggaattcat ggcgacgggc aaggatgcaa
     4981 tggagatggg cgggaagttc accgtgatgt tcgccgacgt tgactgagcc gacacatccc
     5041 cgcccgtctt cgccggacga gctcggcttc gagtcgatcg tctacgagaa ggcgcccccg
     5101 cgcgcaacga tcactctgaa ccggccggaa atcctgaacg ccttcgactt ccggatgctg
     5161 cgcgagctcg cgcgcgcctg cgaggacgca tcgtgggacg acgagatccg cgtggtcgtc
     5221 ctgaccgggg cggggcgtgc gttctgcgtc ggtgccgacc tgaagtcctg gtcggccgac
     5281 tatctcggca agccaaagga gtactggaag tggttcggcg ccttcaagga cgcgcacgac
     5341 cggctgcgcg agatcggcaa accgaccgtg gcgcggatca acgggatcgc ggtcggcggc
     5401 ggcaacgagc tccagatggc atgcgacctc gccgtgatgg tggaggacgc gttcatccgg
     5461 cacgtcggcc tcgagcacgg ttctgtgccg gcgggaggcg cgacgcaatg gctcccgatc
     5521 ttcgtcggcg atcggcgcgc acgcgagatc atcatgctct gcgaggagat tcccgcggcg
     5581 aaggcggccg agtgggggct gatcaatcgc gctgttcctg cagccgagct cgatgccgcg
     5641 gtcgacgaat acgtggctaa gctcgcgacg aagcttccgc agaccacgcg ctacgcgaag
     5701 cagcagctca acttctggcg cgacctgtcc tggcacgaga ccgtgaacca cgcgcgggac
     5761 tggctcgcac tgtccatgct cggcgacgaa gcacaggagg cggtgaaggc atttctgaag
     5821 cgctcgtcct cgtagagcgc gccgcgccga tcgccgtcgt gctgctcaac cgtccgcagc
     5881 agctgaacgc cctgtcgagc gagctgatgg aggagctcgt cgacacgctg cgccggttgg
     5941 acgcggacga cgcggtgggc tgcatcgtcg tcggcggaaa cgaacgggcc tttgcggccg
     6001 g
//
LOCUS       DASVEU010000237         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_751035,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000237 DASVEU010000000
VERSION     DASVEU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751035"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1312
                     /locus_tag="VF225_10185"
     CDS             269..1312
                     /locus_tag="VF225_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX7145662.1"
                     /translation="MRRRRLAYVCALVMLFTLSPATAAAKPGTSWAQAELKTVVAAGI
                     MAKAAAARPEDPLTRGELETIVAGLMHTEAAPPSSTSLAKVTMAALDARLVNALGLAD
                     SAKLFTQAAKTAGLAPPSRFGTEAVARLLGLRTNHPASLDTLELSPGQPATHAEAAYS
                     AARVLQLGPQDTEGVQELAASFILPALTPWQKKVLTTAIRFIGYPYVWGGESEFTEPA
                     ESPFGPQVHGGFDCSGFVWRVYKVEQYADGGTLQDMLEGRTTFAMSAEVPAAKRIAFA
                     KLQPADVIFFGAGGAKSKPKQVDHMGLYLGNGWFIHSSKYGVAVATLSGWYEKRFAWG
                     RRPLAEAGLVSLP"
     gene            1534..>2328
                     /locus_tag="VF225_10190"
     CDS             1534..>2328
                     /locus_tag="VF225_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7145663.1"
                     /translation="MSSQAVTSRRLNALILPVCLAGLGVLIAAGYAFATTAHSGRALI
                     SLAALTAAATLAERFPVPINAETGGGVLSLTFVFAVAAIVLFGWASGALLLLAATAIV
                     QLAQHRPLQRIAFNVAVLALVALAAGLLIAPIHGQSVGAVVARVVLAATADNVVNIVL
                     ISAAIALSSGQPYLQLMRTHIRATIVPFAFMASAALMLVVLWERSPVLSVALVGPLLA
                     IALYQRSTHRALRAMRLALTDPLTGLGNHRQFHERLQRELLRAEEQA"
BASE COUNT          359 a          822 c          724 g          423 t
ORIGIN      
        1 ccggctcgcc gccaggtcga gggggcagca ctccgagctt cctcttcaag cgcctcgagg
       61 attgcccggc gctcctcgtc gctgggttcg ggggtgatcc ggatgtccat gttcgtaact
      121 aaggcgccga ctcggcggcg ccgcagagag gtcgcgcggg ctttgcctgg acggccgtat
      181 tgaccagttt gtcggctcca caaacgctcc tcaccgcccg caccagccgc acattaaagg
      241 gaaacggcga gccgccgata ggacaggaat gcgccgccgc cgccttgcgt acgtctgcgc
      301 gctcgtcatg ctcttcacgc tgtcgccggc aacagccgcc gccaagccgg ggacatcgtg
      361 ggcacaggcc gagctcaaga ccgtagtcgc cgccggaata atggccaagg cggcggcagc
      421 gcgacccgaa gatcctctca cccgcggaga gctcgagacc atcgtcgcgg gcctgatgca
      481 taccgaggcc gccccgccga gctcgacgag cttggcgaag gtcacgatgg ccgctctcga
      541 tgccaggctt gtgaatgcac tcgggctcgc cgatagcgcc aagctcttca cgcaggcggc
      601 caagaccgca ggtcttgcgc ctccctctcg attcgggacc gaagccgttg cgcgcctgct
      661 cggtctccgc acgaaccacc cggcctctct cgacacgctc gagctctcgc ccggccagcc
      721 ggccacccac gccgaagccg cctactcggc agctcgggtc ctgcaactcg gcccgcagga
      781 caccgagggc gtccaggagc tcgcggcatc gttcatcctg ccggcactga cgccctggca
      841 gaagaaagtc ctcacgacgg caatccgctt catcggctac ccgtacgtct ggggcgggga
      901 gagcgagttc accgaacccg ccgaatcgcc cttcgggccg caggtccacg gcggcttcga
      961 ctgctccgga ttcgtctggc gcgtctacaa ggtcgagcaa tatgcggacg gcggcaccct
     1021 ccaggacatg ctcgagggcc gcacgacgtt tgcgatgagc gcagaggttc ccgcggcgaa
     1081 acgcatcgcc ttcgcgaagc tccagcccgc cgacgtcatc ttcttcggag ccggcggcgc
     1141 gaagtcgaag cccaagcagg tcgaccacat gggcctctac ctcggcaacg gctggttcat
     1201 ccactcgtca aagtacggag tcgccgtcgc gaccctctcc ggttggtacg aaaaacgctt
     1261 cgcgtgggga cgccgtccgc tcgccgaagc gggtctcgtt tccctccctt agaagcagtg
     1321 tttttccaaa tcccccgtag gggggatacg acactccgac tgcaaaagaa acaatccacc
     1381 cgtctggcgt ctttgtgttt tagggaggtt tggggatgtt gcagtggaag ccgaagatga
     1441 ttgccctcgt ggcggtcctg gtgctcgtcg cggcagtctc gggtcagttc acctgggact
     1501 cgttcgacca attcacctgg ttctaaccct gacgtgagct cacaggcggt cacgtcccgc
     1561 cggcttaacg ccctcattct ccctgtctgt ctggcgggcc tcggtgtcct catcgcggcc
     1621 ggttacgcct tcgcgaccac agcacacagc ggtcgcgcgc tgatcagcct cgcagcgctc
     1681 accgcggctg cgacgctcgc cgagcgcttc cccgtgccga tcaacgcaga gaccggcggc
     1741 ggcgtgctct cgctcacgtt cgtgttcgcc gttgccgcga tcgtgctctt cggctgggcc
     1801 tccggcgctc tgcttctcct ggcggcgaca gccatcgtcc agctcgcgca gcaccgaccg
     1861 ctccagcgga tcgcgttcaa tgtcgcagtt ctcgctctcg tcgccctcgc cgcaggtttg
     1921 ttgatcgctc ccatccacgg ccagagcgtc ggtgcggtgg tagcgcgagt cgtgctcgcg
     1981 gccactgccg acaatgtcgt caacatcgtg ttgatctctg ctgcgatcgc gctcagtagc
     2041 ggccagccgt acctccagct gatgcggacg cacatccgcg cgaccatcgt gccgtttgcg
     2101 ttcatggcgt ctgcggcgct gatgctcgtc gtcctgtggg agcgttcgcc ggtcttgtcg
     2161 gtggcgctcg tcgggccgct actcgcaatc gcgctgtacc agcgctcgac gcaccgtgcg
     2221 ctacgggcga tgcggctcgc gctcacggat ccgctgaccg ggctcggcaa tcaccgtcag
     2281 ttccacgagc ggctccagcg cgagctgctg cgtgcggagg agcaggcg
//
LOCUS       DASVEU010000238         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1334097,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000238 DASVEU010000000
VERSION     DASVEU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334097"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1848)
                     /locus_tag="VF225_10195"
     CDS             complement(28..1848)
                     /locus_tag="VF225_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7145664.1"
                     /translation="MNTWVEPERRWREDGYAEIRDYATIGDGRAVALVARDGAIDWLC
                     MPDIDSPSVFAALLDAERGGSFELRPSERYHVERRYRPGTNVLETTFHTDGGMARVVD
                     VMTVGGSGLTPFRELARRVEGLTGQVSLTWRIEPRFGYAGGETRIERRFGIPIATSGA
                     SALAAQCWNADEWTTDAGAIGGRIEVRRGESALIALSLSDKEPLVFSSREQVETRIDA
                     TARAWAQWSAGRRYSGPWRDAVIRSALALKLLVYAPSGAPAAAATTSLPEDVGGERNW
                     DYRFSWVRDSAFTMSAFLGLGCAEDARAYFWWLMQASQLTHPRLRVLYRLDGGARAPE
                     RTLALRGYRASRPVRIGNAAAAQLQLDTYGELLQTAWIYAITGHKIEADIARRLAEIA
                     DLVCLIWRQPDAGIWEVRSQPEHFTHSKMMCWIALDRACNLAERGLIPAGRVRRWRRE
                     AQSIRDFVETRCFSQEKRSYVRHAGSAELDASLLLGLLGGYGDATTDRWTGTIEAVRR
                     ELGNGSFVRRYTGDDGLDGSEGAFLACSFWLAEALARCGLVESAVGLMDQLVGLANDV
                     GLYAEEIAPTTSAFLGNFPQGLTHLALINAALAIAEAVTW"
     gene            2165..>2500
                     /locus_tag="VF225_10200"
     CDS             2165..>2500
                     /locus_tag="VF225_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145665.1"
                     /translation="MQGLQEVDVVDGEPVAASLIPRAVTVGELLFDDGATQTFDVGGD
                     TSYVEADGRPTQGKWYVDEDGRFCSFWPPTYRACYDLRWIVEGNEIVGLSFTELGRGT
                     RFDGRYTRLA"
BASE COUNT          410 a          842 c          845 g          403 t
ORIGIN      
        1 agccgccggc gatcgcgccc cagacgctca ccacgtgacc gcctcggcga ttgccagcgc
       61 ggcgttgatc aaagccaggt gcgtcaggcc ctgcgggaag tttccgagga aggcactggt
      121 tgtcggcgct atctcctccg catacagccc tacgtcgttg gcgagcccga cgagctgatc
      181 catcaggccg acggcactct cgaccaggcc gcagcgagcc agggcttccg cgagccagaa
      241 cgaacacgcg aggaacgcgc cctcggaccc gtcgagcccg tcgtcaccgg tgtagcggcg
      301 cacgaacgag ccgttcccga gctcgcggcg caccgcttcg atcgtcccag tccatctgtc
      361 tgtcgtagcg tcgccgtagc cgccgagaag gccgagcagc aggcttgcgt cgagctcggc
      421 ggagccggcg tggcgtacgt agcttcgctt ctcctgcgag aagcagcggg tctccacgaa
      481 gtcgcggatc gactgcgcct cccgacgcca gcgtcggacg cgcccggccg ggatcaggcc
      541 acgttcggcc agattgcacg cgcggtcgag cgcgatccaa cacatcatct tcgaatgggt
      601 gaagtgctca ggctggctcc tcacctccca gatcccggcg tcgggctgcc gccagatcag
      661 gcagacgaga tccgctatct cggccagacg tcgagcgatg tcagcctcga tcttgtgccc
      721 ggtaattgcg tatatccacg cggtctgaag cagctcgcca taggtatcca gctggagctg
      781 cgctgcggcg gcgttgccga ttcgcacagg gcgtgaggct cggtaaccgc gcagggccag
      841 ggtccgctcc ggtgcgcggg cgccaccgtc caagcgatag agaacgcgga gtcgcgggtg
      901 ggtcagctgc gaggcctgca taagccacca gaagtacgcg cgcgcgtcct ccgcgcaacc
      961 gagccccagg aacgcgctca tcgtgaaggc ggaatcgcgc acccatgaaa agcgatagtc
     1021 ccagttacgc tctccgccga cgtcttcggg caacgaggtc gtcgctgccg cggcgggcgc
     1081 tccggatgga gcgtaaacga gcagcttgag cgcgagcgcg ctgcggatca cggcgtcgcg
     1141 ccatggaccg ctgtaacggc ggcccgctga ccactgggcc cacgcccgcg cggtggcgtc
     1201 gatgcgcgtc tccacctgct cgcgtgatga gaagacgagc ggctccttgt cgctgaggct
     1261 gagcgcgatc agcgccgact caccgcggcg tacctcgatg cgtcctccga ttgcgccggc
     1321 gtcggtggtc cactcgtcgg cgttccagca ttgggcggcg agcgcgctgg cgcccgaggt
     1381 tgcgatcggg atcccgaacc gccgctcgat acgagtctcc cctccggcat agccgaagcg
     1441 tggctcgatg cgccaggtca gggaaacttg cccggtaagc ccctcgaccc gacgggccag
     1501 ttcgcgaaac ggtgtgagcc ccgatccgcc caccgtcatc acatcgacga cgcgcgccat
     1561 cccaccgtcg gtatggaaag tggtctcgag cacgttcgtg cctggccggt agcgccgctc
     1621 gacgtggtaa cgctcgctgg gtcgcagctc gaagctgccg ccgcgctcgg cgtcaagcag
     1681 cgctgcgaac acgctgggcg aatcgatgtc cggcatgcag agccagtcga ttgccccgtc
     1741 acgcgcgact agggccacgg cccgaccgtc gccgatcgtg gcgtaatcgc ggatctctgc
     1801 gtacccgtcc tcccgccagc gccgttccgg ctcaacccac gtgttcacac cgagcggatc
     1861 cggggcttct ttggggcgcg gcgggatcgg cggacatggc ggcaccgtgc ccgggtcagg
     1921 agctgatcgg tggtcgcgaa gctgacggcc cgtgcgaggc ggtggccgag cgtttgagcc
     1981 agtcgcgcct gagctgccat gcccgactgg gtcgattccc ccgagtcagc cgtcgcgtcg
     2041 gtgtcgtcca agcgcgccac tctctgtgag gatcactccg gcaccttcga gcgcaacagg
     2101 acaaagcgat gagcgatatc agttgcgttc gaaccagccc ggatccaggc aatagggtga
     2161 tcctgtgcaa ggcctgcagg aagtcgacgt cgttgacggc gagccggttg cggcctccct
     2221 gatcccgcga gcagtgacgg tcggcgagct gctgttcgac gacggggcga cgcagacgtt
     2281 cgatgtaggc ggggacacga gctacgtcga ggcggacggt cgtcccacgc agggcaagtg
     2341 gtacgtcgac gaggatggcc gcttctgctc gttctggccg ccgacctatc gagcttgtta
     2401 cgacctgcgc tggatcgtcg agggcaatga gatcgtcggg ctgagcttca ccgagctggg
     2461 ccggggtact cggttcgacg gtcgatacac aaggctggct
//
LOCUS       DASVEU010000239         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_945374,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000239 DASVEU010000000
VERSION     DASVEU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945374"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF225_10205"
     CDS             <1..1016
                     /locus_tag="VF225_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145666.1"
                     /translation="ARKAAEAVSEAQRAFADHPWPEGVELRVRMGLHTGEPTIRDGAY
                     FGLDVHRAARIAQAGSGGQILLSQRTHDALKASHELADLGEHELPGLDEPVQLFQLNI
                     PGLPSRFPPLSTARKGFRGVRVVLADDSVLLREGIARLLEDAGFEIVAQSGTAEDLLR
                     HVGMHKPDVALIDIRMPPTQTDEGLRAAKQIRERWPDTGVLVLSQYVEPAYAMELLGE
                     NAEGVGYLLKDRVSDVDVFAAAVRRVAEGGSALDPAVVSQLVGRRRRDDPIDELTPRE
                     REVLGLMAEGRSNQAIAEKLVITLRAVEKHVTSIFSKLRLPASAEDHRRVLAVLTYLG
                     STN"
     gene            1139..>2073
                     /locus_tag="VF225_10210"
     CDS             1139..>2073
                     /locus_tag="VF225_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7145667.1"
                     /translation="MSPLKRSNNIAARMGRWSASHWKTAVFGWLAFVVAALFIGNFVG
                     TKNIETADANVGQAHKADEILKHAFPQIDPQTELVLVQSSSKTVKDPAFRATIKDVES
                     TIAGNAAIKNVRSPLDPAHADQVSKDGRSAMVLWDMKGTYENAKPKIDGIVAAVGKAA
                     DRHPGFYVQEAGSVSSGKALDKMFSDQLKLAGERSIPITIVVLLLVFGALVAVGVPLL
                     LALSGVMATIGLVALPSHIVPMDQNVSAVILLVGLAVGVDYALFYLKREREERAAGRS
                     PRAALEAAAATSGRSVLISGITVMVAMAGMMFSGD"
BASE COUNT          356 a          717 c          674 g          326 t
ORIGIN      
        1 acgcccgcaa agcagccgag gcagtctcgg aagcccagcg cgcattcgcg gaccacccct
       61 ggccggaggg cgtcgagctc cgcgtccgca tgggcctcca caccggggag ccgacgattc
      121 gcgacggcgc gtacttcgga ctcgacgtcc atcgcgcagc gcgcatcgcc caggcgggca
      181 gcggcggcca gatcttgctc tcgcagcgaa cccacgacgc gctcaaggcg tcgcacgagc
      241 tcgccgatct gggcgagcac gagctgcccg ggctcgacga gcccgtgcag ctcttccagc
      301 tcaacatccc gggcctgccc tcgcgctttc cacccctcag caccgcacga aagggatttc
      361 gcggcgtgcg cgtagtgctc gcggacgact ccgtcctgct ccgtgagggg atcgcccgcc
      421 tgctcgagga cgccggcttc gagatcgttg ctcagagcgg cacagccgag gatctcctgc
      481 ggcacgtcgg catgcacaag cccgacgtcg ccctgatcga catccggatg ccgccgacgc
      541 agacggacga gggcctgcgc gccgcgaagc agattcgcga gcgctggccc gacacgggtg
      601 tgctcgtcct ctcgcagtac gtcgagccgg cctacgcgat ggaactgctc ggcgagaacg
      661 cggagggcgt cggttacctg ctgaaagatc gcgtctccga cgtggacgtg ttcgcggctg
      721 cagtgcggcg ggtcgccgaa ggtggatcgg cgctcgatcc ggcggtcgtg tcgcagctag
      781 tcggacgccg ccggcgtgac gatccgatcg acgagctgac gccccgcgag cgcgaggtgc
      841 tggggctgat ggcggaaggc cgctcgaacc aggcgattgc ggagaagctc gtgatcacgc
      901 tccgcgcagt cgagaagcac gtgacgagca tcttttcgaa gctccgcctg ccggcgagcg
      961 cggaggatca ccgccgcgtg ctcgcggtcc tcacgtacct cggctccacc aactaggcgt
     1021 actttccccc cgctccgggt gcggttctcc gcctgggaag aggcctgcga gccgcatttc
     1081 cactccggcg tgccgctgcg atcgtgggct tacccccaag cgaaggagct gagtccagat
     1141 gtcacccttg aagcgctcga acaacatcgc ggcccgtatg ggtcgctgga gcgcgagcca
     1201 ctggaagacc gcagtcttcg gttggctcgc gttcgtcgtt gccgcactct tcatcggcaa
     1261 cttcgtcggc accaagaaca tcgaaactgc agacgcaaac gtcggccagg cgcacaaggc
     1321 cgatgagatc ctcaagcacg ctttcccgca gatcgatccg cagaccgagc tcgtgctcgt
     1381 tcagagctcg agcaagacgg tcaaggatcc ggctttccgc gcgacgatca aggacgtcga
     1441 gagcacgatc gcaggcaacg ccgccatcaa gaacgtgcgc tcgccgctcg atcccgcaca
     1501 tgccgaccag gtctccaagg acggccgctc ggccatggtc ctctgggaca tgaagggcac
     1561 gtacgagaac gcaaagccga agatcgacgg aatcgtcgcc gcagtgggca aagcagccga
     1621 ccggcatcct ggcttctacg tccaggaggc cggctcggtg agttccggga aggcgctcga
     1681 caagatgttc tccgaccagc tcaagctggc cggcgaacgc tccatcccga tcacgatcgt
     1741 cgtcctgctg ctcgtcttcg gcgccctcgt cgccgtcggc gtgccgctcc tgctcgcact
     1801 ctcgggcgtc atggcgacca tcggcctcgt cgcactaccg agccacatcg tgccgatgga
     1861 ccagaacgtc tcggcagtga tcctgctcgt cgggctcgcg gtgggcgtgg attacgcgct
     1921 cttctatctc aagcgcgagc gtgaggaacg ggcagccggc agaagcccgc gagccgcgct
     1981 cgaagccgca gccgcaacgt caggccgctc ggtgctgatc tcgggcatca cggtcatggt
     2041 cgcgatggcc gggatgatgt tctccggcga caa
//
LOCUS       DASVEU010000240        11941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_752593,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000240 DASVEU010000000
VERSION     DASVEU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752593"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="rnpB"
                     /locus_tag="VF225_10215"
     ncRNA           <1..300
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF225_10215"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            506..1126
                     /locus_tag="VF225_10220"
     CDS             506..1126
                     /locus_tag="VF225_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7145668.1"
                     /translation="MQALYQQRIYRLLTRDSGLATRTLALVPRDLAKVSRDLLAAQRE
                     LYRLTPPIGVQTIKVARPRPAPQLLSYYREAQQRFKVPWNVLAAVNFVETKFGKLRSA
                     SAAGAQGPMQFMPATWGRYGLGGNVQDAHDAILGAANYLHASGAPQDLRTALHHYNPS
                     SAYVDAVLRYARRIGADRPMFYALYSWQVFVKTPRGDRRVTGPGLP"
     gene            complement(1183..1896)
                     /locus_tag="VF225_10225"
     CDS             complement(1183..1896)
                     /locus_tag="VF225_10225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145669.1"
                     /translation="MKDPVALCAAAVAGWHSAWLAALGLRSAADVDAWHALDQAPHIY
                     FSAITLRSDTPADAVADAAGAVCDSWQNVDLERFGFRDWRHEPWFLCTPSEPSDGETP
                     AELEIVRVTTAQEVEELEAVSVRGFGNEDDTIEPGTIHPPAILDDPRMVLWLGRAEGK
                     PVGAAMSYRTDEAVGIFGVTTIASARRRGYGSALTRAAMLVDSGLPSILAPSPEGEGV
                     YRRLGFEHVGELRIWSRPR"
     gene            complement(1914..3284)
                     /locus_tag="VF225_10230"
     CDS             complement(1914..3284)
                     /locus_tag="VF225_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7145670.1"
                     /translation="MRQDSRDDFQAWLWGEILLFGLLGSSLALFVSNHELQTKFSLPQ
                     LGLVLQTVMAFAGGIIALLAGIRFAVEGRRLDLFLCTGFLIASVSIVAFAIAPVLDGQ
                     PVHRAEAWAGVAGRLLAWTLIAAAPFVQGRSGPRALANTVVAACIALVLGWLALRSSS
                     SVLPGIDPHSETPGLLTGTLAAQALLNLLAVVGFGKRFYERREDLDRWLALGSTLMLF
                     ASLNYVFTPVLAAGAVSRGDALRLLAYGLLLAGAWRAISYAEFGRAVAEERARVAREI
                     HDGLAQYLFAISTHASMLQNGAPQEETIAKLREAANAAQQEARFAILALSTAAGNAPF
                     DAALRRYVEFLTADGQLEVDLEIDSDVRLAPDEQIEVFRIVQEGLANVRKHANATRAE
                     VVIGMRNYERFVLVRDNGEGFDGESPAAGQGLKNIRARTKTIEGGFTLTSKPGLGTAL
                     EVVLRT"
     gene            3335..3979
                     /locus_tag="VF225_10235"
     CDS             3335..3979
                     /locus_tag="VF225_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145671.1"
                     /translation="MAERILLVDDHPLTRSALAGLLAQHGFDVVGEASDGEEAIEAAA
                     RLEPDLILLDLSMPGLDGLSALPRLREAAPQCEVVVLTASGTEENLLGAIRGGAAGYL
                     LKSEPPERIVDFLRGVAQGEAALSGAVARRLLEQVREGGGRSSGVPDSIAAALSAREL
                     EVLLLLDDHLATDEIAKRLFISEHTVRSHVKSLLRKLGVSSRREALEVLSIARG"
     gene            complement(4130..4993)
                     /locus_tag="VF225_10240"
     CDS             complement(4130..4993)
                     /locus_tag="VF225_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7145672.1"
                     /translation="MDVLTHPALAQLHPTFGHPDSPERLEVLLAAFPQAREGHAAERT
                     AVERVHAPEYLDMLEALTEPALLDADTVASETSWTVALLAAGIAIDAAESSGFALVRP
                     PGHHARPGTAMGFCLVNNVAVAARHMQAVHGVDRVAIVDYDVHHGNGTQEIFLEDDTV
                     LFVSLHQWPFYPGSGGPTEQSETTVNVPLSAGSGDEHYLLAFDEVVAPAVEQFHPDLV
                     LVSAGFDAHAEDPLAQMRVTADGFRELSSRCAALAPRVAAVLEGGYNIATLPGLVAAA
                     LAGFSEKTTAG"
     gene            complement(5007..5837)
                     /locus_tag="VF225_10245"
     CDS             complement(5007..5837)
                     /locus_tag="VF225_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX7145673.1"
                     /translation="MKPLLVIDGDSLAHRAYHALPKSMRRAEGKPSNALVGFSNFLMR
                     LWEAEAPRAAIVGWDTLFVPTYRHELFAGYQGGREFDDELLEQLDLLPELVRSFGFVA
                     AKGEGYEADDFLAAAVAQEEKNKGTAVVVTSDRDSFQLASERTTILQPTRGVSELARV
                     GPAEVRERYGVDPGQVPDFIALRGDPSDKLPGARGVGPKTAANLLAQYGTLEAMLEDG
                     RFEAQAEELRMYRRMATLDASAPLPPLVDQTPNWAEASSFVRQLGMNAQADRIAAQAA
                     "
     gene            complement(5834..6448)
                     /locus_tag="VF225_10250"
     CDS             complement(5834..6448)
                     /locus_tag="VF225_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7145674.1"
                     /translation="MRPSLLYSLAAAFSWPVVRWLYRLRAEGEENLPAEGGYVLAANH
                     ISNLDPWPLGLPLWPRRFLRFMAKSELYWWPLGPVITAGGGFPVRRGEHDLEAMNTAI
                     DLAREGNVVAMFPHGTRQRKGLVKRYQPKAHTGAARIALEAGVPLVPAAIAGTDRLSK
                     LGPLRVRYGKPIPLDDLKGEDPSEAAHEATDRLMAEIDRLGADL"
     gene            6504..8816
                     /locus_tag="VF225_10255"
     CDS             6504..8816
                     /locus_tag="VF225_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEX7145675.1"
                     /translation="MAKTLVIAEKPSVGRDLAGALEGPFQRRKLDDIKPKRGKKKAEE
                     PTNEEVVEAAKSTAKTRTTRDEAVFLESDEYVITWAVGHLVQLAEPEEYDEKWKKWKM
                     ADLPIVPPNGFKLVPRDAKSKKQLKLIETLLKREDVDKIINACDAGREGELIFAYVYE
                     SVFGSSATSPKAPKPVERLWISSMTKQAIREGFEKLRPGTQMQSLEDAARSRSEADWL
                     VGMNATRAATIRGRAWVGGVVSLGRVQTPTLGLIVKREREIQAFVPEAYRLIHGTFQP
                     PYQGLWFEGDETRIFGDLTRADAIVAKVSGQDGTVEKVERKEQSERAPLLYDLTSLQR
                     DANRRYGFSARRTLQAAQSLYESKKAITYPRTSSRFLSGDMVPQLKPTAATLQPIEDY
                     RKGAEYVLALDQLPLGRVVNDAKVSDHHAVIPTDIEKDLEAFTPDERRVFDLVAKRFL
                     AVFHPPARYARTTVITLVEEERFRTRGKITLEAGWRGVYGLEPDQERQSEDEAEGGEI
                     PVLQEGQTVRCIAAEIEDKMTKPPPRYTEATLLSAMETAGKLIDDEELREAMKESGRG
                     TPATRAETIETLIRREYIERVGKDLQATPKGIQVITMLLDHKLTSPELTGDWEHKLSD
                     IEHGRGDRKKFMKGIADFAKETVDQIAALDKEQLRPERVELGLCPRCGAETGEIIKEN
                     SKAYGCTSWKSREEVGCGFVIWKRVASRTLTPEIARQLIEERRTREVLSGFRSRNGKP
                     FRARLVLNDEDKIEFEFPVRAQSKEPAATE"
     gene            8944..10014
                     /locus_tag="VF225_10260"
     CDS             8944..10014
                     /locus_tag="VF225_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145676.1"
                     /translation="MTQNQLHELLDSDQAKALVEGAEERGHLEAAELEAFALEHDLNE
                     AEVEELTRELERIGLDIRQPQAEAKVEKEEKPVKPEPHVLSGSADSLQLFLADVGRHK
                     LLTAAEEVTLAKAIERGDPTAKRRMIESNLRLVVSIAKGYRGLGVPFLDLIQEGTLGL
                     NRAVEKFDWRRGYKFSTYATWWIRQSVQRAVANHARTIRVPVHVVERQQKLSRAARRL
                     EVELGREATKDELAEATGLPIQHVDEALGAAQASVSLNQTVGADDEGELGDLFADREA
                     ADPFDEAEESLRRQGVRRALDALPERERRILELRFGFEGEPWTLEAIGHELDLTRERV
                     RQLEGQALARLSALRDLISLAA"
     gene            10379..11932
                     /locus_tag="VF225_10265"
     CDS             10379..11932
                     /locus_tag="VF225_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145677.1"
                     /translation="MVFAGAADPTYLDPALVSDGESFRVTEQIFESLVKLKPGTTLIQ
                     PALAKSWTSKNAKDWTFSLRQGVKFHDGTPFNAAAVCYNYNRQYNFTGAFQDASATYY
                     WQTVFGGFKHNASTDLSPSLYTGCTTHGKYSVTIHLRTPSGPFLPGQVISSFGIQSPT
                     ALKKYGADSGELRNGTFYPTGSYAFSHPTGTGPFKFGSWTVGEKVVLNANPTYWGPKP
                     KIKTLIIRPIANNSARLQALQTGEVQGYDLVAPEHIGTIQSDSKLKVLNRPAFNVAYV
                     TIHQGTGSPMNDIKVRQAVAYGLDRASVVKSFYSGRGQVAREFQPPQLFGWSNKVTQY
                     PYNPTKAKDLLNSSSCHVPCKIDFWYPTGVSRPYMPDPKRNFEAFAASLENSGFSVTA
                     HSAPWRPDYVKHVNDGTAGDLNMIGWTGDFGDPDNFLGTFFQTYSPQFGFRNPAIFNV
                     LAKAEREKSLAKRIALYKQANILIMKYLPAVPYAHSRPALGFEKTVKGYVPSPVGTDP
                     FEGVSIGGQ"
BASE COUNT         2151 a         3965 c         3963 g         1862 t
ORIGIN      
        1 acggctcgag gaaagtccgg acaccgcagg gcaggacgct ggggcgaccc aggcggcgaa
       61 agccgacgga aagtggcaca gaaaggagac cgcctgcagc gattctccgg agacgctgaa
      121 ggtaagggtg aaaaggtgga gtaagagccc accagcgtcg tggcgacacg gcggctagcc
      181 aaaccccgtc cggtgcaagg caaacaggtc ccgccatcag gcggcccgcc gagggaccgg
      241 gttgctgcat agagggatgg tcaccgcaaa cagaatccgg cttacaggcc tgctaaccag
      301 aagcccgcgc atcgcgcggg cttctgcatt tttttgtgcg ctggtcgtcg tcggcagcgc
      361 cgccgcggcc ccgccgagcg tcaacgcacc gatcccgaca aagcctgagc ccctggcccg
      421 cacgcttgtc cggacaactt cggatctcga gcaggcaatc ggcgactgga acccaaagag
      481 catcgccgcg ccccaagccg tggtcttgca ggctctctac cagcagcgga tctacagatt
      541 gctcacgcgt gactcgggtc tcgcgacgcg cacactcgcg ctcgtgccgc gcgatctggc
      601 aaaggtgagc cgagatctcc tcgccgcgca gcgcgagctc taccgtctca ctcctccgat
      661 cggtgtccag acaatcaagg tcgctcgccc acgcccggca ccacagttgc tgagctacta
      721 ccgagaggca cagcagaggt tcaaggtccc atggaacgtg ctcgcggccg tgaacttcgt
      781 agagacgaag ttcggcaagt tacgcagcgc aagcgcggcc ggcgcgcagg gcccaatgca
      841 gttcatgcct gcgacgtggg gccgatatgg gctcggaggg aatgtgcaag acgcgcacga
      901 tgcgatcctc ggcgcagcca actacctgca cgcttcaggc gcaccgcagg atctgcgaac
      961 cgcgctgcac cactacaacc cgtcaagcgc gtacgtcgat gccgtccttc gctacgcgcg
     1021 acgcatcggc gccgatcgac cgatgttcta cgcgctgtac agctggcagg tctttgtcaa
     1081 gactccccgc ggcgaccgtc gcgtcactgg gccgggtctg ccataggaga gaagtgaggt
     1141 tctggccggg tccgcctcgt ttcgaccacg aggtggcccc cgctacctgg ggcggctcca
     1201 gatccgcagc tcgcctacgt gctcgaagcc gagccgccgg tagacacctt ctccttcggg
     1261 gctcggcgcg agaatcgagg ggagcccgct gtcgaccagc atggcggcgc gcgtgagagc
     1321 gctcccgtac ccgcgacgtc ttgcagacgc gatcgtcgtg accccgaaga tgccgacggc
     1381 ttcgtcggtg cggtaactca tcgcggcgcc gaccggcttg ccctcggctc gcccgagcca
     1441 caagaccatg cgcgggtcgt cgaggatcgc gggcggatga atcgtgcccg gctcgatcgt
     1501 gtcgtcctcg ttgccgaacc cgcgcacact gaccgcctcg agctcttcca cctcctgcgc
     1561 agtcgtgact cgtacgatct cgagctccgc cggcgtctcg ccgtcggacg gctctgacgg
     1621 cgtgcagaga aaccacggct cgtgcctcca gtcgcggaag ccgaatcgct cgaggtcgac
     1681 gttctgccac gagtcgcata ccgcaccagc tgcgtcggcg accgcgtccg ccggcgtgtc
     1741 ggagcggagc gtgatcgcgc tgaagtagat gtgcggcgcc tggtccagcg cgtgccatgc
     1801 atcgacatcg gccgccgacc gaagtccgag cgccgcgagc cacgcggaat gccagccggc
     1861 aacagcagcg gcgcacagcg ctaccgggtc cttcatgcgc gaagcgtacg cggttaggtt
     1921 cgcagcacga cttcgagcgc cgtgccgaga ccgggcttcg aagtgagcgt gaatccgccc
     1981 tcgatcgttt tcgtgcgggc gcgaatattc ttcaggccct gcccggccgc cggcgattcg
     2041 ccgtcgaagc cttcaccgtt gtcgcggacg agcacgaagc gctcgtagtt gcgcatgccg
     2101 atcacgacct cggctcgcgt ggcgttcgcg tgcttgcgca cgttggccag gccctcttgc
     2161 acgatgcgga agacctcgat ctgctcgtcc ggcgccagcc tgacgtcgga gtcgatctcg
     2221 aggtcgacct cgagctgccc gtccgcagtc aggaactcga cgtaccggcg cagggcagcg
     2281 tcgaacggtg cgtttcctgc tgctgtggac agtgcaagga tcgcgaagcg cgcctcttgc
     2341 tgcgcagcgt tcgcggcctc ccggagcttt gcgatcgtct cctcctgcgg cgccccgttc
     2401 tgcagcatcg atgcatgcgt cgagatggcg aagaggtact gcgcgagccc gtcgtggatc
     2461 tcgcgcgcga cccgtgcgcg ctcctcggcg accgcgcgcc cgaactcggc gtagctgatc
     2521 gcgcgccacg cgcccgcgag cagcagtccg tatgcaagta gccgcaacgc gtcgccgcgc
     2581 gagacggcgc ccgccgcgag cacaggcgtg aacacgtaat tcagcgacgc aaacagcatc
     2641 aaggtcgagc ccagcgcaag ccagcggtcg aggtcctcgc gtcgctcgta aaagcgtttc
     2701 ccgaatccga ccaccgccag gagattcagg agtgcctgag cggccagcgt gcccgtcagc
     2761 aggccgggag tctcggagtg cggatcgatc ccggggagga cggagctcga ggagcgcagc
     2821 gcgagccaac cgagaacgag cgcgatacac gcggcgacca ccgtattcgc gagtgctcgc
     2881 ggtcccgacc gcccctgcac gaacggcgct gccgcgatca gcgtccaggc gagcaggcgg
     2941 ccggcaacgc ccgcccaggc ctccgctcgg tggaccggct gaccgtcgag gaccggcgcg
     3001 atcgcgaagg cgacgatgga caccgaggcg ataaggaaac cggtgcagag gaagaggtcg
     3061 aggcgccggc cctcgacggc aaaccggatc ccggccagca gggcgatgat cccacccgcg
     3121 aacgccatga ccgtctgcag cacgaggccg agctgcggca gggagaattt cgtctggagc
     3181 tcgtggttgg agacgaagag cgcgagcgag gatccgagca ggccgaacag gaggatctcc
     3241 ccccacagcc aggcctggaa gtcgtctctg ctgtcttgtc gcatgttcac tcttgtcggc
     3301 agatagcagt ccgccgataa gccttgaaac cctgatggct gagcgaatcc ttctcgtcga
     3361 cgaccacccc ctcacccgct cggccctggc cgggctgctg gcgcagcacg gcttcgacgt
     3421 ggtcggggag gcttccgacg gcgaggaggc gatcgaggcg gccgctcggc tcgagccgga
     3481 cctgatcctc ctcgacctct cgatgcccgg cctcgacggc ttgagcgcgc ttccgcgatt
     3541 gcgcgaggct gctccgcagt gcgaggtcgt cgtcctcacc gcctccggaa cggaggagaa
     3601 tctgctcggt gcaattcgcg gcggtgcggc cggctacctc ctgaagagcg aaccgcctga
     3661 gcggatcgtg gatttcctcc gcggcgtggc acagggggaa gccgcgctct ctggagccgt
     3721 ggcgcgccgg ctcctcgagc aggtgcgtga gggcggcggg cggagctcgg gtgtgcccga
     3781 ttcgatcgca gcagcgctgt cggcgcgtga gctcgaggtg ctgctgctcc tcgacgacca
     3841 tctcgccacc gacgagatcg ccaagcggct gttcatctcc gagcacaccg tgcgctcgca
     3901 cgtgaagagc ctgttgcgaa agctcggcgt ctcctcgcgc cgagaagcgc tcgaggtgct
     3961 ttccatagcg cggggctgac cgtctcactc attttgggtg agtgccttcc cccgagccgg
     4021 gcgatgcttc cagagggctc gctgccgata cttcgggcag atctacacgc tccccgggac
     4081 ggagcgaagt ggagaacgac cagtggcgaa cgacttcgac ggccggcttt cagccggccg
     4141 tcgtcttttc cgagaaccct gccaacgccg cagccaccag gcccggcaag gttgcgatgt
     4201 tgtagccacc ctcgagcacg gcggcaactc ggggcgcaag cgctgcgcag cggctactga
     4261 gctcgcgaaa gccgtccgcg gtgacgcgca tctgcgcaag cgggtcctct gcgtgtgcgt
     4321 cgaagccggc agacaccagg accagatcgg ggtggaactg ctcgaccgcc ggggcgacca
     4381 cctcgtcgaa cgccaggagg tagtgctcgt caccggagcc ggcgctcaaa ggaacgttca
     4441 cggtcgtttc gctctgttcg gttggaccgc cgctgcccgg gtagaacggc cactgatgca
     4501 gcgagacgaa cagaaccgtg tcgtcctcca agaagatctc ctgcgtgccg ttcccgtggt
     4561 ggacgtcgta gtcgacgatc gccactcggt cgacgccatg cacggcctgc atatgccgag
     4621 cagcgacggc gacgttgttg acgagacaga agcccatcgc agttcccggc cgggcgtgat
     4681 ggcccggcgg ccgcaccagg gcaaaaccgg agctctcggc agcgtcgatt gcgatccctg
     4741 cggccagcag cgccacggtc cagctcgtct ccgaggcgac cgtatcggcg tcgagcaagg
     4801 ccggttccgt cagtgcctcc aacatgtcca ggtactcggg cgcgtgtaca cgttcaactg
     4861 cagtgcgttc cgcagcgtga ccttcgcgcg cctgagggaa cgccgctagc aggacctcga
     4921 gccgttcggg cgaatccggg tggccaaacg tggggtggag ctgcgcgagc gccgggtgag
     4981 tcaggacgtc gatcggcgga cggctcctac gcggcctgcg cggcgatcct gtcggcctgc
     5041 gcgttcatgc ccagctgccg tacgaaggaa gacgcctccg cccagttagg ggtctgatct
     5101 acgaggggag gtaggggggc ggaggcgtcc agggtcgcca ttcgacggta catccggagt
     5161 tcctccgcct gcgcttcgaa gcggccgtcc tcgagcatcg cctcgagtgt gccgtactgc
     5221 gccaggaggt tcgccgccgt cttcggcccg acgccgcgcg cgccggggag cttgtccgac
     5281 gggtcgccgc gcaacgcgat gaagtccggt acttgccccg ggtcgacgcc gtagcgctcc
     5341 cgcacctccg caggccccac acgggcgagc tcgctcacgc cgcgcgtcgg ttggagaatc
     5401 gtcgtccgct cgctcgcgag ctggaacgag tcgcggtcgg atgtgaccac gacggcggtg
     5461 cccttgttct tctcctcctg cgcgacggcg gccgcgagaa agtcgtcggc ctcatagccc
     5521 tcgcccttcg cagcgacgaa gccgaacgaa cggacgagct ccggaagaag gtcgagctgc
     5581 tcgagcagtt cgtcatcgaa ctcacgccca ccctggtacc ccgcgaagag ctcgtgcctg
     5641 tacgtaggca cgaagagcgt gtcccagccg acgatggcgg cgcgcggcgc ctccgcctcc
     5701 cagaggcgca tgaggaagtt cgagaacccg acgagggcgt tgctcggctt gccctcggcc
     5761 cgccgcatcg acttcggcag tgcatggtac gcgcggtggg cgagggaatc gccgtcgatc
     5821 acgagcagcg gcttcacaga tccgcaccca ggcggtcgat ctccgccatc agccgatcgg
     5881 tcgcctcgtg cgccgcctcg gaagggtcct cgcccttcag atcgtccagc gggatcggct
     5941 tgccgtagcg cacgcgcagc ggcccgagct tcgagagccg gtccgtcccc gcgatcgccg
     6001 ccggcacgag cggcacgccg gcctcgagcg caattcgagc ggcgcccgtg tgcgctttgg
     6061 gctggtaccg cttcacgagc cctttccgct gccgcgtgcc gtgcgggaac atcgccacca
     6121 cgtttccctc gcgcgcgagg tcgatcgcgg tgttcatcgc ctcgaggtca tgctcgccac
     6181 gccggacggg gaaaccgccg ccggccgtga tcaccgggcc caagggccac caatagagct
     6241 ccgatttcgc catgaagcga aggaaccgcc tcggccacag cgggagccct agtggccagg
     6301 gatcgaggtt cgagatgtgg ttcgccgcga ggacatagcc gccttcggcc gggaggttct
     6361 cctcaccctc ggcacgcaat cggtacagcc agcgcaccac cggccaggag aaggcggcgg
     6421 cgagggaata gaggagactg ggccgcaccc ggccaagtat ccccggcccg gcgcccattc
     6481 cgagggacgc tatggtgttt tggatggcca agacccttgt cattgcagag aagccctcag
     6541 tcggccgcga tctcgcgggc gcgttggaag gacccttcca gcggcggaag ctcgacgaca
     6601 tcaagccgaa gcggggcaag aagaaggctg aagagccgac gaacgaagag gtcgtcgagg
     6661 ccgcgaagtc gacggccaag acccgaacca cccgcgacga ggcggtcttc ctcgagtccg
     6721 acgagtacgt gatcacgtgg gccgtcggcc atctcgtgca gcttgccgag cccgaggagt
     6781 acgacgagaa gtggaagaag tggaagatgg ccgacctgcc gatcgtcccg ccgaacggct
     6841 tcaagctcgt gccgcgcgat gcgaagtcga agaagcagct gaagctgatc gagacgctgc
     6901 tgaagcgcga ggacgtcgac aagatcatca acgcatgtga cgccggccgc gagggcgagc
     6961 tgatcttcgc ctacgtctac gagtcggtct tcggcagctc ggcaacgagc cccaaggcgc
     7021 cgaagcccgt cgagcgactg tggatctcgt cgatgacgaa gcaggcgatc cgcgaaggat
     7081 tcgagaagct ccggccgggc acgcagatgc agtctctgga ggatgcggcg cgctcccgct
     7141 cggaggccga ctggctcgtc ggcatgaacg cgacgcgtgc ggcgacgatt cgcggccgcg
     7201 cctgggtcgg cggcgtcgtc tcgctcggcc gggtgcagac gccgacgctc gggctgattg
     7261 tcaagcgcga gcgcgagatc caggcgttcg tccccgaggc gtaccgtttg atccacggga
     7321 cgttccagcc gccgtaccag gggctctggt tcgagggcga cgaaacgcgg atcttcggtg
     7381 acctcacgcg cgccgacgcg atcgtcgcca aggtctccgg ccaggacggg acagtcgaga
     7441 aggtcgagcg caaggagcag tccgagcgcg cgcctctgct ttacgacctg acctcgctcc
     7501 agcgcgacgc gaaccggcgt tacggcttct ccgcgcggcg aacgctgcag gccgcgcagt
     7561 ccctctacga gagcaagaag gcgatcacgt atccgcgcac atcttcgcgc tttctctccg
     7621 gcgacatggt cccgcagttg aagccgacgg ccgccacgct gcaaccgatc gaggactacc
     7681 gcaagggagc cgaatacgtg ctcgcgctgg accagctgcc gctcggacgc gtcgtcaacg
     7741 acgcgaaggt gagcgaccat catgcggtca tcccgaccga catcgagaag gacctcgagg
     7801 ccttcacgcc cgacgagcgc cgtgtgttcg acctcgtagc gaagcgcttc ctcgcggtct
     7861 tccatccgcc ggcgcgctat gcccgcacga ccgtgatcac gctcgtcgaa gaggagcgct
     7921 tccgcacgcg cggcaagatc acgctcgagg ccgggtggcg cggcgtctac gggctcgagc
     7981 ccgaccagga gcgtcagtcg gaggacgagg ccgaaggcgg cgagatcccg gtgctgcagg
     8041 aaggccagac cgtgcgctgc atcgcggccg agatcgagga caagatgacg aagccgccgc
     8101 cgcgctacac cgaggcgacg ctgctctcgg ccatggagac cgccggcaag ctcatcgacg
     8161 acgaggagct gcgcgaggcg atgaaggagt ccgggcgagg cacgccggcg acacgcgccg
     8221 agacgatcga gaccctgatc cggcgcgagt acatcgagcg cgtcggcaag gacctccagg
     8281 cgactccaaa gggtatccag gtcatcacga tgctccttga ccacaagctc acctcccctg
     8341 agctcacggg tgactgggag cacaagctct ccgacatcga gcacgggcgc ggggatcgca
     8401 agaagttcat gaagggaatc gccgatttcg ccaaggagac cgtcgaccag atcgctgccc
     8461 tcgacaagga gcagctccgg ccggagcggg tcgagctcgg gctttgtccg cgctgcggcg
     8521 ccgaaacggg cgagatcatc aaggagaact ccaaggcgta cggctgcacg agctggaaga
     8581 gccgggagga ggtcggctgc ggttttgtga tttggaagcg ggtcgcgagc cggacgctta
     8641 cgccggaaat tgcgagacag ttgatcgagg aacgtcggac ccgggaagta ctctcaggtt
     8701 tccgctcacg gaacggcaaa ccttttcgtg cgcggctcgt cttgaacgac gaggacaaga
     8761 tcgagttcga gtttcctgta cgtgcgcaat ccaaagagcc tgcggcgacg gaataacaaa
     8821 ggacccggtc acgttgcacc gttttgaccc ttctgggacg gctgaggcct ggaaataaga
     8881 ggggtttcgg cctacagtgc gaaggttgcc gccgtatcac aaagtaggac tcgaaaggga
     8941 cacttgacac aaaatcagct gcacgaactg cttgattctg accaggccaa ggcgctcgtc
     9001 gagggcgctg aggagcgtgg gcacctcgag gctgcagagc tcgaggcgtt cgccctcgag
     9061 cacgatctca acgaggccga ggtcgaggag ctgacccgtg agctcgagcg gatcgggctc
     9121 gatatccgcc agccacaagc cgaggcgaag gtcgagaagg aagagaagcc cgtcaagccg
     9181 gagccgcacg tcctctcggg ctccgcagac agcctccagc tcttcctggc cgacgtcggc
     9241 cgccacaagc tgctcaccgc cgcggaagag gtcaccctcg ccaaggcgat cgagcgtggc
     9301 gaccccaccg ccaagcgacg catgatcgaa tcgaacctgc gcctggtcgt ttccatcgcc
     9361 aagggctatc ggggcctcgg cgtcccgttc ctggacctga tccaggaggg cacgctcggc
     9421 ctgaaccgcg ccgtcgagaa gttcgactgg cgacgtggtt acaagttctc gacctacgcg
     9481 acctggtgga tccgccagtc ggtgcagcgc gccgtggcga accacgcccg gacgatccga
     9541 gtgcccgttc acgtcgtcga gcgccagcag aagctgtcac gcgcggctcg ccgcctcgag
     9601 gtggagctgg gccgcgaggc gacgaaggac gagctcgccg aggcgaccgg ccttccgatc
     9661 cagcatgtcg acgaggcgct cggcgccgcg caggcctccg tttcgctgaa ccagacggtc
     9721 ggtgcggacg acgagggcga gctcggagac ctcttcgccg atcgcgaggc cgcggatccg
     9781 ttcgacgagg ccgaggaatc gctccggcgc cagggcgtcc ggcgggcact cgacgccctg
     9841 cccgagcgcg agcgccgcat cctcgagctc cgcttcggct tcgagggtga gccctggacg
     9901 ctcgaggcga tcggacacga gctcgacctg acgcgcgagc gcgttcggca gctcgagggc
     9961 caggctctcg cccgcctttc ggcgctacga gatctgatct ctctcgccgc ttagcggcgg
    10021 cgttatctga tcggacctga gggcggccta tacgggccgc cctcaatcga tacggcgcga
    10081 aatccggccc gctttcgcgg gccatgatga tttcgcgccg gtctgctgtc ggcgagccgc
    10141 agtgggagag ccgggcaaag cccgtctctc cggcgctact tccatcgttt ccgcggcaaa
    10201 agcgtcttag gttgaggagg gcttaacctt ccgttcataa ttgtgcgtcc gcccgaaggg
    10261 cgggacgacg aaaggagttc tgttgagggg gaatttgaga aggctggcct tgccgctcgc
    10321 tgcgctttgc gtcttggttg tcgcgagcac ggcaggcgga gcctcgaaag caacaacgat
    10381 ggtctttgcc ggcgcggcgg atcccacgta tctggatccg gcgctggttt cggacggtga
    10441 gtcgtttcgt gtcaccgagc agatcttcga gagcctcgtc aagctgaagc cgggcacgac
    10501 cttgatccag ccggcgctgg cgaagagctg gacctcgaag aacgcgaagg actggacgtt
    10561 ctcgctgcgt cagggcgtga agttccacga cggcacgccg ttcaatgcgg cagccgtttg
    10621 ttacaactac aaccgccagt acaacttcac cggtgcgttc caggacgcgt ccgccacgta
    10681 ttactggcag acggtgttcg ggggcttcaa gcacaacgcc agcaccgacc tgtcgccgag
    10741 tctctacacc gggtgcacga cgcacgggaa gtactccgtc accatccacc tacgcacccc
    10801 gtcgggtccg ttcctgccgg gtcaggtcat ctcgtcgttc gggatccaga gcccgaccgc
    10861 gctgaagaaa tatggggcgg acagcggtga gctgcgtaac ggcacgttct atccgaccgg
    10921 gtcatacgct ttcagccatc cgaccggcac cgggcccttc aagttcgggt cgtggacggt
    10981 gggcgagaag gttgtcctaa acgcgaatcc cacctactgg ggaccgaagc cgaagatcaa
    11041 gacgctgatc atccggccga tcgccaacaa ctcggcccgt ctgcaggctc tgcagaccgg
    11101 tgaggtacag ggttacgacc tggtcgcgcc ggagcacatc ggcacgatcc agtcggactc
    11161 gaagctcaag gtcctgaaca ggccggcgtt caacgtcgcc tacgtgacga tccaccaggg
    11221 cacgggctcc ccgatgaacg acatcaaggt ccgccaggca gtcgcctacg gacttgaccg
    11281 cgcgtcggtc gtcaagtcgt tctactccgg ccgaggacag gtcgcgaggg agttccagcc
    11341 gccccagctg ttcgggtggt cgaacaaggt cacgcagtac ccgtacaacc cgacgaaggc
    11401 gaaagacctg ctcaactcgt cgagctgcca cgtgccgtgc aagatcgact tctggtatcc
    11461 caccggggtc tcacggccgt acatgcccga cccgaagcgg aacttcgaag ccttcgccgc
    11521 gagcctcgag aactcgggct tcagcgtcac ggcgcacagc gcaccgtggc ggccggacta
    11581 cgtcaagcac gtcaacgacg gcacggccgg cgacctgaac atgatcggtt ggacgggcga
    11641 tttcggtgac ccggacaact tcctgggcac gttcttccag acctacagcc cgcagttcgg
    11701 gttccgcaac cctgccatct tcaacgttct cgcgaaggct gaaagggaga agagcctcgc
    11761 aaagcgcatc gcgctctaca agcaggcgaa catcctgatc atgaagtacc tccctgccgt
    11821 gccgtacgcg cattcgcgcc cggcgcttgg attcgagaag acggtcaagg gctatgtccc
    11881 gagccccgtc ggaacggatc cattcgaagg cgtttccatc ggaggacagt aggaactgag
    11941 c
//
LOCUS       DASVEU010000241         6917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_368712,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000241 DASVEU010000000
VERSION     DASVEU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368712"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..852
                     /locus_tag="VF225_10270"
     CDS             25..852
                     /locus_tag="VF225_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7145678.1"
                     /translation="MRSIALALGASLTWGFADFFGALKGRTLGALRTLVYVQLGGLVA
                     IALIVAIRWKGPADLDALFAIPAAVSGTLGLYAFYRGMAVGAMSIVAPIAGISAAVPV
                     VFGIATGDRPSLWQWLGIAGALGGVFLASREPGRGVKVAAGVGLALLAAIGFGGYFPP
                     MHAAGNADFWWASLIFRMTSTSIILAAVAIRRPSLAVAPMQVPLLALVGVGDMLGNLL
                     FAAASTSGLVSITSVLASLYPIITVLLARLVLKERVARSQEAGIVLTLAGVALISAG"
     gene            complement(913..1269)
                     /locus_tag="VF225_10275"
     CDS             complement(913..1269)
                     /locus_tag="VF225_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145679.1"
                     /translation="MNVLFVCKQNAGRSQMAHTLYERRGGNARSAGTTPAARVHPEVA
                     ELMPELADRVPKGLDSADAEWADIVVTMGCGDACPVIPGKRYIDWELQDPAGQDVETV
                     RKIRDEIERRVAELPL"
     gene            complement(1266..1592)
                     /locus_tag="VF225_10280"
     CDS             complement(1266..1592)
                     /locus_tag="VF225_10280"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7145680.1"
                     /translation="MKSIAVLPTICCGPETEPLTIPERDALASRFKALADPTRVAIIN
                     CLSAADEVCVCNLTETFDLSQPTISHHLKILRDAGLVESSRRGTWAYYRLVPEAITAL
                     RGALGT"
     gene            complement(1703..2611)
                     /locus_tag="VF225_10285"
     CDS             complement(1703..2611)
                     /locus_tag="VF225_10285"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX7145681.1"
                     /translation="MALEVGIVGLPSSGKSTLFHALTGARATGDVGMAGVPDPRLAQI
                     AGVIHPRKVTPAAIRVVEVRGTGPELLGNLRQVDALLVVLDGFSGTRVPADDLETLKL
                     ELLVADRDHVERRLERVTKQAKSGEQKLKQEVAELERVLAHVDAGGTLADWSAELPPG
                     LEPLTTKPLLAVENGPGGIDLKLEAELAEMPADEAVAFREGASALEEVVRRLSDELGL
                     ISFFTAGDKETRAWSLRRGRTALEAAETIHTDIARGFIRVEVIRWEDLVRAGSHAEAA
                     RSGLQRLEGKTYVVQDGDVLNVRFNV"
     gene            complement(2614..3207)
                     /locus_tag="VF225_10290"
     CDS             complement(2614..3207)
                     /locus_tag="VF225_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7145682.1"
                     /translation="MADEATEAAARPAKDSTAHIRRLVERGQQGEREALEELYLIHFD
                     RIYSYLHMSVGNRHDAEDLTTQTFLKMLESIKRFRWQSAPFSAWLFRIAHNLAMDHFR
                     ANRRWQPEDEVPEPEGESEPSAEAAALQSIGRQSMLELIEGLSTEQQQVLTLKFVFNL
                     PNADVATILDKTEGAVKSLQHRALVSLQKQISRSTEP"
     gene            complement(3257..3511)
                     /locus_tag="VF225_10295"
     CDS             complement(3257..3511)
                     /locus_tag="VF225_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145683.1"
                     /translation="MAEPERVRIEIAFDGGQIMGALVTPAGADALEQALGSAELRAGT
                     GEAALALEAEDGRYTIALQRVVYVKRFARESRVGFGSAVA"
     gene            complement(3504..3908)
                     /gene="mce"
                     /locus_tag="VF225_10300"
     CDS             complement(3504..3908)
                     /gene="mce"
                     /locus_tag="VF225_10300"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7145684.1"
                     /translation="MQARGIHHLGVAVEDLESALQTYERLFGARVVHRATVEDQGVRA
                     ASVRIGESRVELLEPLGEDTPVGRFLAKRGPGMHHVAYEVNDLDTALAELARAGADLI
                     DERPRPGLFGLEVAFVHPDSVHGVLSEVVASG"
     gene            complement(3936..5465)
                     /locus_tag="VF225_10305"
     CDS             complement(3936..5465)
                     /locus_tag="VF225_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7145685.1"
                     /translation="MDLHSYMVALRDFVVNWWPLLGLTFYCVLLYIFWRMMQLMPRVK
                     PATVIVDPRTAIEWGDIAGADEAKAELLEIVEFLRDPKRFHELGAVVPKGVLLYGPPG
                     TGKTLLARAAANESGAKFYAQSASAFVEMFAGLGAARIRKLFAEARKHSPAIIFIDEL
                     DAVGTARSGNSFNREHDQTLNQLLVELDGFASGDQVVVMGASNRLQDLDPALLRPGRF
                     DRQLLVAPPDRKGREAILHVHTRAKPLSSDVQLDLIARQTSGLTGADLANIANEAAIL
                     AGRREAKYVSALDFDNALERVVAGLQQKKVLTDKERRILAYHEAGHALMSHLMGEVGH
                     LQKVTIVSRGTALGYTLHLPNEDRYLESKEELIDMLKIALAGRAAEQVVFGRVTNGAA
                     SDLEKVTEIARAMVFEWGMSDIVTSRTLRADNYALSEETKRVRDSEQAHLTDGAYTEA
                     LRLIEKHRASLDRLSQALLEKETLMREEVLLLLTDVEPESRASETVGTPRAVVSLPAK
                     A"
     gene            complement(5521..6126)
                     /locus_tag="VF225_10310"
     CDS             complement(5521..6126)
                     /locus_tag="VF225_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX7145686.1"
                     /translation="MTILWDVDTQFDFMLPDGKLYVPGAEETVPVMKRLVDAARAAGI
                     VHVASADDHELTDAEISAEPDFVTTYPPHCLRGTRGARKIPETDQEDPVPLALEPVPD
                     RYLEGREFLLLKKNFDVFTNPHTERLLERLDPDEIVVFGVATDVCDDAAIRGFLARGL
                     RVRFVEDAARGLGDDRVAISTASWREQGVEFTKAEVIAGTF"
     gene            complement(6123..>6917)
                     /locus_tag="VF225_10315"
     CDS             complement(6123..>6917)
                     /locus_tag="VF225_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145687.1"
                     /translation="VMTIEGDYTLFAHLETAYLGVLARRTLITTNVVRVLEAAGGKPI
                     IFMPARHDHHRVQTGDGYAAYVAGRIVGAPIGVTTDEQASWWGGRGIGTVPHSLIASY
                     GGNTVLAATKFAEWASDDINITVLVDFENNSVQTALDVARTLGPKLWGVRLDTSEMLV
                     DRSLWEELGDFKPTGVNERLVRKVRDALDNDGFEHVKIVVSGGFTIDKIAQFEARGVP
                     VDAYGVGSSLIRGSNDFTADIVLTDGRPSAKVGRRYKPSQRLELVA"
BASE COUNT         1096 a         2326 c         2268 g         1227 t
ORIGIN      
        1 cttgcagcta ggtagcgtcg ggcgatgaga tcgatcgcgc ttgcgctcgg cgccagcctg
       61 acctggggtt tcgcggactt cttcggagcg ctcaaggggc gaacgctcgg agcgctgcgc
      121 acgctcgtct acgtgcagct cggggggctc gtcgcgatcg ccttgatcgt cgcgatccgg
      181 tggaaagggc ctgcggacct cgatgcgctc ttcgcgatcc cggcagcggt ctcgggcacg
      241 ctcggcctgt acgcgttcta tcgagggatg gccgtcggcg caatgagcat tgtggcgccg
      301 atcgcaggca tctccgccgc ggtgccggtc gtcttcggga tcgcgaccgg cgaccgtccg
      361 tcgctctggc agtggctcgg gatcgccggc gccctcggcg gcgtcttcct cgcgtcacga
      421 gagccgggcc ggggcgtcaa ggtggcggcc ggcgtcggcc tggcgttgct ggcggcaatc
      481 gggttcggcg gttatttccc tccgatgcac gcggcgggga acgccgactt ctggtgggcg
      541 tcgttgatct tccgcatgac gtcgacttcc atcattctcg cggcggtcgc gatccgtcgc
      601 ccgtcacttg ccgtcgcacc gatgcaggtg ccgctgctgg cgctggtcgg ggtcggtgac
      661 atgctcggca acctgctctt cgcggccgcg tctacgagcg ggctcgtcag catcacgtcg
      721 gtgctcgcgt cgctctatcc gatcatcacg gtcctgctgg ctcgcctggt cctcaaggaa
      781 cgcgtagcgc gatcgcagga agcgggcatc gtgttgacgc tcgccggcgt cgcgctcatt
      841 tcagccggtt agtcgcaacg ccttcgtcac acttttcggg tctgacccca aaatgtgttt
      901 tggtacgtcg aattacagcg ggagctcggc aacgcgacgc tcgatctcgt cacggatctt
      961 tcggacggtc tcgacgtcct ggccggcagg atcttgcagc tcccagtcga tgtagcgctt
     1021 gccgggaatg accgggcagg cgtcgccgca acccatcgtc accacgatgt ccgcccactc
     1081 ggcatcggcg gagtcgagcc ctttcggaac gcggtccgcg agctcaggca tgagctcggc
     1141 cacctccgga tgtacacgcg ccgccggagt cgtccccgcc gagcgtgcgt tgccaccgcg
     1201 ccgctcgtag agcgtgtggg ccatctggga acgaccggcg ttctgcttgc agacgaaaag
     1261 gacgttcacg tgcccagcgc tccgcgcagc gcagtgatcg cttccggcac caggcgatag
     1321 taggcccacg tcccacggcg cgacgattcc accaggccgg cgtcacggag gatcttcagg
     1381 tggtgcgaga tcgtcggttg tgagagatcg aacgtttcgg tgaggttgca cacgcacacc
     1441 tcgtcggccg ccgagagaca gttgatgatg gcgacgcgcg tcgggtcggc gagcgccttg
     1501 aaccgcgacg caagcgcgtc gcgctcggga atcgtgagcg gctcggtctc ggggccgcag
     1561 cagatggtgg ggaggacggc aattgacttc atcgactctt ttcaatatat ctacatcgac
     1621 ggatatcgat gaaaggaggc gaggatggcc gcttggtgcg actgcccaga cgactgctgg
     1681 cccgacggct gttgctgatg ggctagacgt tgaagcgaac gttcaggacg tcgccgtcct
     1741 gaaccacgta cgtcttacct tcgagacgtt ggaggccgct tcgcgcggcc tccgcgtgtg
     1801 agccggccct cacgaggtct tcccagcgga tcacctcgac gcggatgaat ccgcgggcga
     1861 tgtcggtgtg gatcgtctcg gccgcctcca atgccgtccg cccgcggcgc agcgaccacg
     1921 cgcgagtctc cttgtcgccg gcagtgaaga aactgatcag cccgagctcg tcgctgaggc
     1981 gacgtaccac ttcctcgagc gcggacgctc cttcgcgaaa tgcgaccgcc tcgtctgcag
     2041 gcatctccgc gagctcggct tcgagcttga ggtcgatccc gccgggcccg ttctcgactg
     2101 cgagcagcgg cttcgtcgtg agcggctcga ggccgggcgg cagctccgcc gaccagtcgg
     2161 cgagcgttcc accggcgtcc acgtgcgcca gcacccgttc gagctcggcc acctcctgct
     2221 tcagtttctg ttcccccgac ttcgcctgct tcgtgacgcg ctcgagccgg cgctcgacgt
     2281 gatcccggtc ggcgaccaac agctcgagtt tcagcgtctc caggtcgtcg gcgggaaccc
     2341 gcgtccccga gaagccgtcg aggacaacga gcagcgcgtc cacctggcgc agattcccga
     2401 gcagctcggg ccccgtgccg cgtacctcga cgactcgaat cgccgcgggg gtgaccttgc
     2461 gtgggtggat gacaccggcg atctgcgcca gccggggatc gggaacgcca gccatgccaa
     2521 cgtcgcctgt cgcgcgagcg cccgtgagcg catggaacag cgtcgacttg ccggagctcg
     2581 gcagcccgac gattccgacc tcgagggcca tcgctacggc tccgtgctgc gggagatctg
     2641 cttctgcaac gagacgagcg cgcggtgctg aagcgacttc accgcaccct cggtcttgtc
     2701 gagaatcgtc gccacatccg cgttgggcag attgaagacg aacttgaggg tcaagacctg
     2761 ctgttgctcc gtcgacagcc cttcgatcag ctcgagcatg ctctgccgcc caatcgactg
     2821 caacgccgcc gcctccgcgg agggctcgga ctcgccctca ggctctggca cctcgtcctc
     2881 gggctgccag cgacggttcg cgcggaagtg atccatggcg aggttgtgcg caatgcggaa
     2941 gagccacgca gagaacggcg ccgattgcca cctgaaccgc ttgatcgact ccagcatctt
     3001 cagaaacgtc tgcgtcgtca gatcctcggc gtcgtgtctg ttgccgacgc tcatgtgcag
     3061 atagctgtag atgcggtcga agtggatcag atacagctct tcgagcgctt cccgctcacc
     3121 ctgctggcca cgctcgacga gacgccggat gtgagcggtc gagtcctttg ccgggcgcgc
     3181 ggcagcctcg gtggcttcgt cggccatcaa cgctgaagtg ccccggtcgg tagggcttgt
     3241 aacccgcagg cccgcctcaa gcgacggccg agccgaaccc cacgcgactt tcgcgcgcga
     3301 atctcttcac gtagacgacc cgctgcaggg caatcgtgta gcggccgtcc tcggcctcga
     3361 gtgccagcgc ggcctcgccc gtacccgcgc ggagctcggc ggagccgagc gcctgttcga
     3421 gcgcatcggc gccggccggc gtgacgaggg cgcccatgat ctggccgccg tcgaacgcga
     3481 tctcgatccg tacccgctcc ggttcagcca cttgcaacca cctccgaaag aacgccgtgg
     3541 acggaatccg ggtggacgaa cgcgacctcg agcccgaaca gaccgggccg cgggcgctcg
     3601 tcgatcagat ctgcgcccgc gcgcgcgagc tccgccagcg ccgtatcgag atcgttcacc
     3661 tcgtaagcga catggtgcat cccgggccca cgcttggcga ggaagcggcc gaccggcgtg
     3721 tcctcgccaa gcggctcgag tagctcgacg cgactctcgc cgatgcgcac cgacgcggcg
     3781 cgcacacctt gatcctcgac agttgcacgg tgcacgaccc gcgcaccgaa caaccgctcg
     3841 taggtctgga gcgccgactc gaggtcctcg accgcgaccc cgagatggtg gataccccgc
     3901 gcctgcacgc gagcgatgct atccgctgag tggttctagg ccttggccgg aagggagacc
     3961 acggcgcgag gcgtgcccac ggtctcggac gctcgcgact cgggctcgac gtcggtcagg
     4021 agaagcagca cctcttcgcg catgagggtc tccttctcga gcaacgcctg gctcaggcgg
     4081 tcgagtgagg cccgatgctt ctcgatcaag cggagggcct ctgtgtaggc gccgtccgtc
     4141 aggtgagcct gctcgctgtc tcgcacccgc ttcgtctctt cggagagcgc gtagttgtcc
     4201 gcgcgcagcg tgcgcgacgt cacgatgtcc gacatccccc actcgaagac catcgcgcgc
     4261 gcgatctccg tcaccttctc cagatcgctc gcagcgccgt tcgtgacccg tccgaacacg
     4321 acctgctcgg ccgcacgacc tgcgagcgcg atcttcagca tgtcgatcag ctcttccttc
     4381 gactccaggt agcgatcctc gttcggcaaa tggagggtgt agccgagtgc ggtgccgcgg
     4441 gacacgatcg tcaccttctg gaggtgtccg acctcgccca tcaggtgcga catcagcgcg
     4501 tggcccgcct cgtggtaggc gaggatccga cgctccttgt cggtcaggac cttcttctgc
     4561 tggagtccgg cgacgacacg ctcgagcgcg ttgtcgaagt ccagagccga aacgtacttc
     4621 gcctcccgac gaccggcgag aatcgccgct tcgttcgcga tgttcgcgag atcggcgccc
     4681 gtcagaccag acgtttgacg cgcaatcaga tcgagttgca cgtccgagga gagcggcttg
     4741 gcccgggtat ggacgtgcag gatcgcctcg cggcccttgc gatccggcgg cgcgacgagc
     4801 aactggcgat cgaagcggcc cggtcgcagc aaggccgggt cgagatcctg cagccggttc
     4861 gatgcgccca tgacgacgac ctggtcgccc gaggcgaagc cgtcgagctc gacgagcagc
     4921 tggttgagcg tctggtcgtg ctcgcggttg aagctgttgc cactacgagc ggtgccgacc
     4981 gcgtcgagct cgtcgatgaa gatgatggcg ggcgagtgct tccgcgcttc agcgaagagc
     5041 ttccggatgc gagccgcgcc gaggccggcg aacatctcta cgaacgccga tgcgctctgc
     5101 gcgtaaaact tcgcacccga ctcgttggcg gctgcgcgtg cgagcagggt cttccccgtt
     5161 cccggcgggc cgtacagcaa gacacctttg ggaaccaccg caccgagctc gtgaaagcgt
     5221 ttcgggtcgc gaaggaactc cacgatctcg agcagctctg ccttcgcttc atcggcgccg
     5281 gcgatgtcgc cccactcgat ggccgtgcgc gggtcgacga tgaccgtcgc cggcttgacg
     5341 cggggcatca gctgcatcat gcgccagaag atgtagagca gcacgcagta gaacgtcagg
     5401 cccaacagtg gccaccaatt gaccacgaag tccctgagcg cgaccatgta gctgtgtaga
     5461 tccacccccg gcatccctta ctcatcatct ccagggaagg gcccgcgtca accgcgcgcc
     5521 ctagaaggtg ccggcgatta cctcagcctt ggtgaattcg accccctgtt cacgccagga
     5581 ggccgtgctg atcgccacgc gatcgtcgcc cagccctcgg gcagcatcct cgacgaagcg
     5641 caccctgagc cctcgagcca ggaagcctcg gatcgctgca tcgtcgcaca catccgttgc
     5701 gacgccgaac acgacgatct cgtcgggatc gaggcgttcg agcagccgtt ccgtgtgcgg
     5761 gttcgtgaag acgtcgaagt tcttcttgag cagcaggaac tcacggccct cgaggtagcg
     5821 atccgggacc ggctcgagcg cgagaggcac cgggtcttcc tggtcggtct cgggaatctt
     5881 gcgcgcaccg cgtgtaccgc gcaggcagtg cggcggatac gtcgtgacga agtcaggttc
     5941 cgccgagatc tccgcgtcgg tgagctcgtg gtcgtcggcg gaggcgacgt gcacgattcc
     6001 ggccgctcgt gcggcgtcga cgaggcgctt catcactgga acggtctcct cggcgccggg
     6061 gacgtacagc ttcccgtcgg ggagcatgaa gtcgaactgc gtgtcgacgt cccagagaat
     6121 ggtcacgcga cgagctcgag ccgctggctc ggcttgtatc tccggccgac cttcgcggac
     6181 gggcgtccgt cggtgagcac gatgtctgcg gtgaaatcgt tcgagccgcg aatcagcgac
     6241 gagccgacgc cgtatgcgtc gaccggcacg cccctcgcct cgaactgcgc gatcttgtcg
     6301 atcgtgaaac cgccggacac gacgatcttc acgtgctcga aaccgtcgtt gtcgagcgcg
     6361 tctcgcacct tgcgcacgag ccgctcgttc acgccggtcg gcttgaagtc gcccagctcc
     6421 tcccacagcg agcggtcgac gagcatctca gatgtgtcga ggcgcacgcc ccacagcttc
     6481 ggcccgagtg tccgcgcaac gtcgagcgcg gtctgcaccg agttgttctc gaagtcgacc
     6541 agtacggtga tgttgatgtc gtccgaggcc cattccgcga acttggtcgc tgcgagcacc
     6601 gtgttgccac cgtacgaagc gattagagag tgcggcaccg ttccgatccc ccggccgccc
     6661 caccaggagg cctgctcgtc ggtcgtgacg ccgatcgggg cgccgacgat acggccggcg
     6721 acgtacgcag catagccgtc tcccgtctgc acgcgatggt ggtcgtgccg cgcgggcatg
     6781 aagatgatcg gcttgccgcc ggcagcttcg agcacccgca cgacgttggt cgtgatcagc
     6841 gtgcgacgcg cgaggacgcc gaggtacgcg gtttccaggt gggcgaacag ggtgtagtcg
     6901 ccctcgatgg tcatgac
//
LOCUS       DASVEU010000242         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1339793,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000242 DASVEU010000000
VERSION     DASVEU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339793"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VF225_10320"
     CDS             complement(<1..1455)
                     /locus_tag="VF225_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7145688.1"
                     /translation="MASVEQPTAAPPKTDGLLLNPRTYDPAALDPESRRVLLATIEWF
                     ESRGKGVLKEHDRDRVWYGDFLEFVKRERVFATLLTPAANSGGDQNKRWDTARICAFN
                     EIAAFYGLAYWYTWQVSILGLGPIWQSENPAVRKRAAGLLDEGAIFGFGLSEREHGAD
                     IYSTDMVLTPDGDGGFRANGGKYYIGNGNLAGIVSVFGRRADMEGEDGYVFFAADSKH
                     PRYHLIKNVVNSQSYVSEFRLEDYPVRPEDVMHVGRAAFDAALNTVNIGKFNLGFASI
                     GICEHAFYEAITHADARVLYGMRVTDFPHVRQSFTDAYARLVAMKLFSDRAIDYMRSA
                     GPEDRRYLLYNPVVKMKVTSEGERVVDLLWDVVAAKGFEKDMYFEMAARDIRALPKLE
                     GTVHVNMALVLKFMTNYLFNPADYPPVPVRRDAADDEFLFRQGPARGLGKIQFHDWRS
                     TYADFGGVPNVARFAEQAVGLCALLTSAPPTEEQQ"
     gene            2186..2434
                     /locus_tag="VF225_10325"
     CDS             2186..2434
                     /locus_tag="VF225_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145689.1"
                     /translation="MAGAGDRLALDLTGAELAAAVRAAIVQRVQMPVVPQKQNGRLAR
                     RPAGRLAVLQLSCVDCRPIARPSSKAVSFTPTPRVNER"
     gene            2431..>2862
                     /locus_tag="VF225_10330"
     CDS             2431..>2862
                     /locus_tag="VF225_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145690.1"
                     /translation="MTAEEAIHRERRAAREAEQPGAPGDAATPRGERRRIQRGEGGAP
                     TAFARRCSRSVGVAQERHELADLLDSVIAAGHVGEPHGWPPGAVAAGRRIKPPPAPCP
                     GDIGSQNGKDGKGAQERDPWVAASLLSLFDASPGVREPVREI"
BASE COUNT          471 a          965 c          975 g          451 t
ORIGIN      
        1 ctgctgctcc tcggtgggcg gggcgctcgt cagcagcgcg cacagcccca cggcctgctc
       61 tgcaaaccgc gccacgttcg gcaccccacc gaagtcggca taggtcgaac gccagtcgtg
      121 gaactggatc ttcccgagac cccgggcggg gccctggcgg aagaggaact cgtcgtcggc
      181 ggcatcgcgg cgcacgggca ccggcgggta atcggccggg ttgaagaggt agttcgtcat
      241 gaacttcagt accagcgcca tgttcacgtg cacggtgccc tcgagcttcg gcagggcgcg
      301 gatgtcgcgt gccgccatct cgaagtacat gtccttctca aagcccttgg cggcgacgac
      361 gtcccagagc aggtcgacca cccgctcacc ctcgctcgtg accttcatct tcacgaccgg
      421 gttgtagagc aggtagcgcc ggtcctcggg gccggccgag cgcatgtagt cgatcgcccg
      481 gtcgctgaag agcttcatcg cgaccaggcg agcgtaggcg tcggtgaacg actggcgcac
      541 gtgtgggaaa tcggtgactc gcatgccgta aaggacgcgt gcgtcggcgt gcgtgatggc
      601 ctcgtagaag gcgtgctcgc agatcccaat cgaggcgaac ccgaggttga acttgccgat
      661 gttcacggtg ttgagcgcgg cgtcgaacgc cgctcgaccg acgtgcatca cgtcctccgg
      721 gcgcaccggg tagtcctcga gccgaaactc gctgacgtag gactgcgagt tcacgacgtt
      781 cttgatcagg tgatatctcg ggtgcttgct gtcggcggcg aagaagacat agccgtcctc
      841 gccctccatg tcggcgcggc ggccgaagac cgagacgatg ccggcgaggt tgccgttgcc
      901 gatgtagtac ttgccgccgt tcgcgcggaa tcccccgtcg ccgtccgggg tgagcaccat
      961 gtcggtcgag tagatgtcgg cgccgtgctc gcgctccgag aggccgaagc cgaagattgc
     1021 gccctcgtcg agcagcccgg cggcgcgctt gcgcacggcg gggttttcgc tctgccagat
     1081 cgggccgagc ccgaggatcg agacctgcca ggtgtaccag tacgccagcc cgtagaaggc
     1141 cgcgatctcg ttgaaggcac agatccgggc ggtgtcccaa cgcttgttct ggtccccgcc
     1201 cgagttcgcg gccggcgtca gcaacgtggc aaacacccgc tcgcgcttga cgaactcgag
     1261 gaagtccccg taccagaccc gatcgcggtc gtgctccttg aggacgccct tgccacggga
     1321 ctcgaaccac tcgatcgtcg ccagcagcac gcgccgcgac tcggggtcga gagcggcggg
     1381 gtcgtacgta cgggggttga gcagaagccc gtcggtcttc gggggggcgg ccgtcggctg
     1441 ctcaacactc gccatgccct ggtctctcct gacggaagcg ggaatgaggc ggggactcta
     1501 cgcgctgtcg gcggacccag tgcgcggagg tttagccgag cagcccacaa aactcgacag
     1561 cgcctgcgga gcaagctgac ggggcctccc gtacggctcc gctagggcat gggagttatc
     1621 agcgcgaggg tgatgccgct agccaaggcc ccggcgagaa caccgcgcga gagcgagcgt
     1681 cgcaaccgta gtgccgtcgg cgtgatcgcc gggcggatcc atcgagcggg aaccagtctc
     1741 aagatcggcg ctttgcgacg cgcgcgtgca acgatgaggt tttccaggcg cctgtcgatt
     1801 gccgcgctga ccgtactgcc catctctcga ggatacgttc gggctcagtc agccgcgacg
     1861 cgcgcaagcc cccgcccacc accgtgcgag ggaccgcact ccgcgcccgc gtgccctgcg
     1921 ctcgggtgat ggcgacggca agccggtgcc gatctgccga gctaccaatt gagctaccaa
     1981 tttgagcgaa actgagccga cctgagcgaa ctggttcagc tccacagagc ctcagctgac
     2041 cccgtatgcc ctggggtccc cttgtggtcc aggcggtcgc gggttcgagt cccgtcgctc
     2101 accctcagag ttcctgcaaa tccagcgttt atgactgcga gcgggtggcg actgaaaccg
     2161 ctcgggctac caattttgtg ccggagtggc aggggcaggt gaccgactcg ccctcgacct
     2221 gaccggcgct gagctcgcag ccgcggtgcg agcagcgatc gtgcagcgcg tgcagatgcc
     2281 cgtcgtgcct cagaagcaga acgggcgtct cgcccgccgt cccgctggtc ggcttgccgt
     2341 cctccaactc tcctgtgtcg actgtcgtcc aatcgcgcgg ccctcgtcga aggccgtctc
     2401 gttcacaccg accccgcgcg taaacgagag gtgaccgccg aggaggccat ccaccgagag
     2461 cgccgcgccg cccgcgaggc tgagcagcct ggcgcgcccg gggacgccgc gacgccgcgc
     2521 ggcgagcgac gccgcataca gcgcggtgaa ggaggagccc cgacggcatt cgcgcgccga
     2581 tgctcgcgaa gcgtcggggt cgcacaggaa cgccacgaac tcgcggacct cctcgacagc
     2641 gtcatcgcag ccggccacgt cggcgaaccg cacggatggc cgccgggcgc cgtcgccgcc
     2701 gggcgacgta tcaagccgcc gccggcgccg tgtccggggg acatagggtc ccaaaacgga
     2761 aaggacggca agggcgctca ggagcgcgat ccatgggttg ccgcctccct gttgtcgctc
     2821 ttcgacgcga gccccggcgt tcgagagccg gtgcgcgaga tc
//
LOCUS       DASVEU010000243         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1728553,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000243 DASVEU010000000
VERSION     DASVEU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728553"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VF225_10335"
     CDS             complement(<1..598)
                     /locus_tag="VF225_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX7145691.1"
                     /translation="MTGPVALGDDVIVNTQAGELELGSGGFDVLYANLTRGLDLLPED
                     DAHVMKLPYTPAQNARRHAEEGGTLPDTLAGLPVLCCSLHSQVVPVCAGIGPGKRLAY
                     VQLAGGALPVSLSDALRALRESGYVETAIAVGACLDGDVECVSAASALLYAAAEEFDV
                     VVCAIGPGIVGTGSTFGHGGLAAADAANAAAALHGMPIL"
     gene            complement(687..1805)
                     /locus_tag="VF225_10340"
     CDS             complement(687..1805)
                     /locus_tag="VF225_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7145692.1"
                     /translation="MASEVVDLFTELAALPSPPGEERAVADVVIRYLRDLGLSVDEDD
                     AGPKVGSNIGNVYCRLEATDGGSPIFLCAHLDTVPPEGPIEPVIEEGVVHNAAGTILG
                     ADNKSAVAAMLEGTRRVLAENCPHGGIELLFTPKEEVGLLGAAAFDLERLHARLGYVY
                     DQAAPIGDVILGAPFSRAMQVRFHGRAAHSGMYPEEGRSAIAAAARAISDLRLGRVDE
                     ETTANVGIIRGGTAGNIVPEWCTLDAEARSHDERKLDELVQEMVDAFSFAAGLEDCEV
                     EAKVSKSYKGYRFKVEDPVVRIAAAALERSGYKPAYGLSGGAADANVFNEHGFACLNL
                     ANGMQDIHTPDERITVADLEGMVEVTLALVAAARDADA"
     gene            complement(1825..>2187)
                     /locus_tag="VF225_10345"
     CDS             complement(1825..>2187)
                     /locus_tag="VF225_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145693.1"
                     /translation="EDLGGTMRLGAQAVELVDGTRAHDAYGEPVVHERHRHRYEVNNH
                     YRPALVEGGLVVSGTFQAGRLVEIIELPEHPWFVASQFHPEFKSRPTRPAPLFRDFVG
                     AALARSRERSAVGAASAT"
BASE COUNT          354 a          771 c          712 g          350 t
ORIGIN      
        1 caaggatcgg catgccgtgc agcgcagcgg cagcgtttgc ggcatcggcg gcggcgagcc
       61 cgccgtggcc gaacgtcgag cccgtgccga cgattcccgg tccgatcgcg cacacgacga
      121 cgtcgaactc ctcggcggcg gcgtagagca gagccgacgc ggccgagacg cactcgacgt
      181 ctccgtccag acaggcgccg accgcgatcg ccgtttcgac gtagcccgac tcgcgcaacg
      241 ctctcaacgc gtccgagagc gagaccggta gcgctccgcc tgcgagctgc acataggcga
      301 gacgtttccc cggtccgatt ccggcgcaca ccggcacgac ctggctgtgc agcgaacaac
      361 agaggacggg caacccggcg agcgtgtccg gcagggttcc tccctcctct gcgtgccgtc
      421 gcgcgttttg agcgggcgtg taagggagct tcatcacgtg ggcatcgtcc tccggaagca
      481 gatcgaggcc gcgcgtcaga ttcgcataga gcacgtcgaa gccgcccgat ccgagctcga
      541 gctcaccggc ctgcgtgttg acgatcacat cgtcgccgag cgccaccggg ccggtcaagc
      601 gcggatacgc gacacagggc tcaccgtcaa cttcgagtcg agagagacct tcgacccgct
      661 cgaggacggc ggttatgcgg ccgcgcctaa gcgtcagcat ctcgagcggc ggccacgagc
      721 gcgagggtta cttcgaccat tccctcgagg tctgccacgg tgatgcgttc gtcgggcgtg
      781 tggatgtctt gcattccgtt cgccaggttg agacacgcga atccgtgttc gttgaagaca
      841 ttcgcgtcgg ctgcgccgcc actcaggccg tacgccggct tgtaaccgct gcgctcgagg
      901 gcggccgctg cgattcggac gacgggatct tcgaccttga agcgatagcc cttgtagctc
      961 ttcgacacct tcgcctccac ctcgcagtct tcgagccctg cggcgaagga aaacgcgtcg
     1021 accatctcct gcaccagctc gtcgagcttg cgctcgtcgt gcgagcgcgc ctcggcgtcg
     1081 agcgtgcacc actccggaac gatgtttccc gcggttccgc cccggatgat cccgacattc
     1141 gccgtcgtct cctcgtcgac acggccgagc cgaaggtccg agatggctcg cgccgccgcc
     1201 gcgatcgccg agcgcccctc ttcggggtac atgccggagt gcgcagcgcg accgtggaag
     1261 cggacctgca tcgcccgcga gaacggcgcg cccagaatga cgtcgccgat cggtgccgcc
     1321 tggtcgtaca cgtacccgag ccgcgcgtgc agacgctcga ggtcgaaggc cgccgcaccg
     1381 agcagaccca cttcctcctt cggagtgaac agcagctcga tgccgccgtg cggacagttc
     1441 tcggccagga cccggcgagt gccttcgagc atggcagcga cagcagactt gttgtccgcg
     1501 ccgaggatcg tgccggccgc gttgtgcacg acaccctcct cgatcaccgg ctcgatcggg
     1561 ccttcgggcg gaaccgtgtc gaggtgtgcg cagaggaaga tcgggctgcc gccgtccgtc
     1621 gcctcgagcc ggcagtagac attgccgatg ttcgagccga ccttcgggcc cgcgtcgtct
     1681 tcgtctaccg acagcccgag gtcgcgcagg tagcgaatca cgacgtccgc aacggcgcgc
     1741 tcctcgcccg gcgggctcgg cagcgccgcc agctcggtga aaaggtcgac gacctcggag
     1801 gccatcgctc gatcttattt gtgcctacgt tgctgaggca gcgccgaccg cggaccgctc
     1861 acgcgaacgc gcgagtgcgg caccgacgaa gtcgcggaac agcggcgccg gacgcgtcgg
     1921 ccgcgacttg aactccggat ggaactggct cgccacgaac cacgggtgct ccggcagctc
     1981 gattatttcg acgagccggc ccgcctggaa cgtgcccgag acgaccagcc ctccttcgac
     2041 gagcgcaggg cggtaatggt tgttgacctc gtagcgatgc cggtgacgct cgtgcacgac
     2101 gggctcgccg taggcgtcat gcgcgcgagt cccgtccacc agctctaccg cctgcgcacc
     2161 gagcctcatc gtcccgccga gatcctc
//
LOCUS       DASVEU010000244         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_954239,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000244 DASVEU010000000
VERSION     DASVEU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954239"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VF225_10350"
     CDS             <1..903
                     /locus_tag="VF225_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145694.1"
                     /translation="GDVSVYWLALALALKTAESAFIGLGWRNILRVAYPRSGLSFKTA
                     WAASQGGTAINALAPAQAGTVAMIGIFRTSIPGSSVAGLAAATAAQALFFTAVSVMML
                     IGVAVLLPRVVSKGTPADETGGFFASHPVLIPAVVVAAIVILIVLWPRLKPWLHEQWR
                     KGKQGLAIFSDWRRYAREVALPSGASYCCRIAVNVVFMAAFDIPITVFTVALVASSHM
                     LSGLFAITPGGVGQTQALDVATLGRHAPTENVAAFSVTQDSILMMWNVVLGVALMLWA
                     FGYAQTRQLLSRPRRKQAQEEGST"
     gene            1041..2198
                     /locus_tag="VF225_10355"
     CDS             1041..2198
                     /locus_tag="VF225_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7145695.1"
                     /translation="MSEIDTGVSSHEDELVRAELDEARQKRRTGRARIVGTLVSVAVI
                     GAVFAFALPRIADYGDVWDEIRKLSWQWLVVLGLATLLNLATYGPPLVAALPGLSYFQ
                     ASRVTLASTALSVVAPGGAAVGMATTVAMLRARGFSGRPVGLAVVVMSIWNQSVILGF
                     PILAVAGLAAAGARNRTLEIAALVGLAVFAVIVAGFAIALSSERVANRVGDRAARLVT
                     AAGRLLHKAPVKWSGASFVRFRDESIELIRRRWPFLTAATLAGHLTVFVVLVVSLRAV
                     GVTHAEVTIVEAFAAWTLARILGAIPITPGGVGFVELGLTAVLVAFGASNAEAVAGTI
                     IYRFLTVVPTVLLGLVAAGTSVVGRKPGETAVTKPATEASRHSGRGRSRPG"
     gene            complement(2845..3351)
                     /locus_tag="VF225_10360"
     CDS             complement(2845..3351)
                     /locus_tag="VF225_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145696.1"
                     /translation="MSNPALFYAIATVSMSFAAFAGLYLALRPHDAAWKEYEVAQVRT
                     IILYGLTALFGALLLVPLADLIGESLALRVMSAGLLVIVFYGHQVRLDTSWTRWPKVR
                     HDLSGRALIIEMAPFVVVAVADQVLLLANLVAPKEGLYQLALILILATPALVFVTVVS
                     RFGSIAEG"
     gene            complement(3437..3697)
                     /locus_tag="VF225_10365"
     CDS             complement(3437..3697)
                     /locus_tag="VF225_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145697.1"
                     /translation="MRIASIASDFAWFARRFLRDRGLDAYVSVSARQCTTEGSPHVDG
                     VDLAVTVSQRDAATRATLATALERELAGKFPDGRVRFHVGAE"
     gene            complement(4000..4254)
                     /locus_tag="VF225_10370"
     CDS             complement(4000..4254)
                     /locus_tag="VF225_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145698.1"
                     /translation="MVRYLYAWTPLVIVGTVVLLSLPWLGLIALVILSLIALAALAAL
                     AWAIVFVPYMLGRAISRHWQGGGGAHQPSAALSLAERKNA"
     gene            complement(4419..>5767)
                     /locus_tag="VF225_10375"
     CDS             complement(4419..>5767)
                     /locus_tag="VF225_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7145699.1"
                     /translation="LLDAAKRLVALDPSLARETCLEALAAAIFAGRLGSGRDVLRVVR
                     AVPPAQPPAASDLLLNGLATLVTQGYAAAVAPLRAALEAFRRDDGASPANDRWLWLAC
                     RVAADLWEHEIWDELATSGVRRAREAGALSVFPISASYHAGVHVHAGEYADASALLEE
                     AYAVAQATHTALLVQARQMVAAWRGNEAQALELIEAGRPDATARGQGMALSMIECANA
                     VLFNGLGHYDEALAPAQRACAQDDLSLYALALVELIEAAVRGNRPELAATALERLSER
                     TRASGTDWALGLEARSRALLTDGPSAEPLYVDAVERLAHGRLAPHLARAQLVYGEWLR
                     RENRRLDAREQLRVAHDTFSRSGAEGFAERARRELSATGETARSRTDDTRSVLTAQEA
                     QIARLARDGLSNPEIGAQLFISPRTVQYHLRKVFQKLDITSRNQLGRLPASRLSSA"
BASE COUNT          887 a         1930 c         1996 g          954 t
ORIGIN      
        1 ggggacgttt ccgtttactg gctcgcgctc gctctcgcgc tgaagacggc cgagtcggcc
       61 ttcatcggac tgggctggcg caacatcctg cgcgtggcgt acccgcgaag tggcctttcg
      121 ttcaagacgg cgtgggcggc atcgcaggga gggacggcga tcaacgcgct ggcgccggcg
      181 caggcgggga ccgttgccat gatcggcatc ttccggacga gcatccccgg atcgtcggtc
      241 gctggcctcg cagcagccac ggccgcccag gcgctcttct tcacggcggt gagcgtaatg
      301 atgctgattg gcgtggcggt actcctcccg cgcgtggtct cgaagggcac gcccgcggac
      361 gagaccggcg gcttcttcgc gtcacacccc gtcctgattc cagccgtcgt tgtcgcggcg
      421 atcgtgatcc tcatcgttct ttggccgcgg ttgaagccgt ggctgcacga gcaatggcgc
      481 aaggggaagc agggtcttgc catcttcagc gactggcgtc gctacgcgag ggaggtggcg
      541 cttccgtccg gcgcctcgta ctgctgtcgc atcgccgtca acgtcgtctt catggccgcc
      601 ttcgacatcc ccatcaccgt gttcacggtt gcgctcgttg cctcttcgca catgctctcc
      661 gggctcttcg cgatcacacc gggtggagtc gggcagacgc aggcgctcga cgttgccacg
      721 ctcggcagac acgctccgac cgagaatgtc gccgcatttt cggtcacgca agactcgatt
      781 ctgatgatgt ggaacgttgt tctcggcgtc gcgctcatgc tctgggcctt cggctacgca
      841 cagaccaggc agctgctctc gaggccacgc cggaagcagg cgcaggagga aggctccacg
      901 tgaacgagtc gctgccaaga ccgggatcgc gtcgctgtgc gagcccccgg ccgaagaatc
      961 cctccagggt gagcttcggc gggtgcgggc caccgcccgg cggccggaag cacccaggct
     1021 aaggaagggg ctaccggtct gtgagcgaga tcgatacggg cgtctcctct cacgaagacg
     1081 agctcgtccg cgccgagctc gacgaggcgc ggcagaagcg ccgcacggga cgcgcgcgga
     1141 tcgtcggcac cctcgtctcc gtcgctgtca tcggggcggt gttcgcgttc gcactaccgc
     1201 gcatcgcgga ctacggagac gtctgggacg agatccgcaa gctgtcgtgg cagtggctcg
     1261 tcgtgctcgg gctcgcgacg ctcctcaacc tcgccaccta cgggccgccg ctcgtggccg
     1321 cgcttcccgg gctctcctac ttccaggcgt cgcgcgtcac gctcgcgtcg acggcgctct
     1381 ccgtggtcgc tccaggcggc gccgcggttg gcatggcgac gacggtcgcg atgctgcgcg
     1441 cgcgcggctt cagcggccgc cccgtcgggc tcgccgtcgt cgtcatgagc atctggaacc
     1501 agtcggtgat cctgggcttc ccgatcctcg ccgtcgcggg ccttgcggcg gcgggggccc
     1561 gaaaccgcac gttggagatc gcggcgctcg tcggcctcgc ggtcttcgcg gtgatcgtcg
     1621 cggggttcgc gatcgccctc tcgagcgagc gggttgcaaa ccgggtcggc gatcgcgccg
     1681 cgcgtcttgt caccgcggct ggacgcctcc tccacaaggc gccggtcaag tggagcggcg
     1741 cgtccttcgt ccgcttccgc gatgagtcga tcgagctcat tcgccggcgc tggccttttc
     1801 tcaccgccgc cacgctcgcc gggcacctga cggtcttcgt cgtcctggtc gtctcactgc
     1861 gcgccgtcgg cgtcacgcat gccgaggtga cgattgtgga ggccttcgcg gcgtggacgc
     1921 tggcgcgcat cctcggcgca atcccgatca cgcccggcgg cgtgggcttc gtcgagctcg
     1981 gtctcacggc tgtgctcgta gccttcggtg cgagcaacgc cgaggcggtc gccgggacga
     2041 tcatctaccg ctttctgacg gtcgtgccga cggtcctcct cgggctcgtc gccgcgggga
     2101 cgtcggtagt cgggcggaag ccgggcgaga cggcggtcac gaagccggca acagaagcgt
     2161 cccggcactc tggccgcggc cgttcgcgac cgggctgaga gaaatcgcct gcaaatcagg
     2221 cagcggtcac gccctatcga cctcagtgcg acctcgcgtc gcctatccgg gcaacggcag
     2281 cgccggataa acccctgcgt ctagctcgcg ccggggcgtc gtgtctcagt gaccaacttc
     2341 tcgagaagct ggttgtgctc ggccttgcgg cactcgcggc gttcgcgttc ggccttattt
     2401 tccgtcttcg cacccgacag acgtgctgcc cgcttgctgc ccgatagcgc ggtcatcgtg
     2461 cctgtcgaga caacgaaagg tgccatttgc aggcagttat gaagacggga gcgacgggac
     2521 tcgaacccgc gacctccggc gtgacaggcc ggtacaggcg caaccgcgac ggccggctgc
     2581 aaccctgatt taccggctgg agcaggcgtt tcgttccgcc tcgaaccggc tgtgaccggc
     2641 cacgggcggc tgccacccgg cagagcccgt gtagtacgtg tgtagtcgtg cgggtgtcaa
     2701 agcaggcaac accggcgtcg cacggggagc tggtggccat ccgaacgggc cagcgtgatc
     2761 gcgtagacga agacgaggtc gagagaacag ctccagcgga gtcacccgct gctcgcgctc
     2821 ggccatcact ggagggactc gcggtcagcc ttcggcgata gacccgaatc gcgagaccac
     2881 ggtcacgaac accagggccg gcgtggcgag gatgaggatg agggcgagtt ggtacaggcc
     2941 ttccttcggc gcgacgaggt tcgccagcag cagcacttgg tcggcgaccg cgaccaccac
     3001 gaacggagcc atctcgatga tcaacgcacg tccggagagg tcgtgtcgaa cctttggcca
     3061 ccgcgtccag gacgtgtcca acctgacctg atgaccatag aacacgatga cgaggagccc
     3121 agcgctcatg acgcggagag ccagggactc gccgatcaag tcggccaacg gaacgagcag
     3181 gagcgcgcca aatagagctg tcagcccgta gaggatgatc gttctgacct gggcaacctc
     3241 gtattccttc caagctgcat cgtgcggccg cagcgcgagg tagaggccgg cgaacgcggc
     3301 gaagctcatg ctgaccgtgg cgatcgcgta gaagagagcc gggttgctca cctcactgaa
     3361 cccctcgtcc agccgccgga acggccttta cgatcgacac cggccgcttc gagtcggcga
     3421 ggacgccgcc gcccgctcac tccgcgccca cgtggaaccg cactcgtcca tcagggaact
     3481 tgcctgcaag ttcgcgttcg agcgcggttg ccagcgtcgc ccgagtcgcc gcgtcccgct
     3541 gcgacaccgt gaccgcgagg tcgacaccgt ccacgtgtgg cgagccttct gtcgtgcatt
     3601 gccgcgccga gacgctcacg taggcatcga gcccacgatc acgaaggaag cgacgcgcga
     3661 accaggcgaa gtcggacgcg attgacgcga tgcgcaaatc gtctggtgtc ggagcgagct
     3721 cgcggctaga gcttggatct gcgagctcga gcaggtgacc ccgaatgctc gcccgaaagc
     3781 catctccgcg gctggatggg atgacgaaca gcggaaaccc tgtgggggaa gaggcggcct
     3841 cgtggattcg ctggtcgtcg agagcgccat gcggagccga tgagaccggg agcgttactc
     3901 ggatgcctac gttcttgcgg ctcgcgagta cgattcgggg cttgtggttg tcggagccac
     3961 tcatcgcact tctccgcttc gtcacacccc gaccgcgagc taggcgttct tgcgctcagc
     4021 cagggacagg gccgcgctag gttgatgggc gccacctccg ccttgccaat ggcgactgat
     4081 cgcccggccg agcatgtacg gcacgaagac gatcgcccac gcgagcgccg ccagcgcggc
     4141 gagcgcgatg agcgaaagga tcacgagcgc aatcagcccg agccacggga gcgacaggag
     4201 aacgacggtc ccgacgatga cgagcggcgt ccatgcatag aggtacctca ccatggttcc
     4261 tcccggattg gagtgggtgc gtttctcacc gttcgagaat cccgcagtgg gcgctgcatt
     4321 cgcatccgcc gagacgccag cgcgcgtcgc tagtcggggt ccgctggctc gttcgctagc
     4381 cggcgtagat cagtcgtttc cgcaacagga acagagggct acgcagagct gaggcggctg
     4441 gcgggaaggc ggcccagctg gttgcgcgac gtgatgtcga gcttctggaa gaccttgcgc
     4501 aggtggtact gcacggtgcg agggctgatg aacagttgag cgccgatctc ggggttggag
     4561 aggccgtcgc gggcgagacg tgcgatctga gcctcttggg ctgtgagcac gctacgcgtg
     4621 tcgtcggtgc ggctgcgcgc ggtctcgccg gtggccgaaa gctcgcgacg ggctcgctcc
     4681 gcaaacccct cggctccgct gcggctgaac gtgtcatggg caactcgaag ttgctcgcgc
     4741 gcgtcgagac gtcgattctc gcgacgcagc cattcgccgt acacgagctg ggctcgggcc
     4801 aggtgagggg ctaggcggcc gtgggccagc cgctcgaccg cgtccacgta gaggggttcg
     4861 gcggaagggc cgtcggtcag caacgcgcgc gagcgggcct cgagcccgag ggcccagtcg
     4921 gtgccgcttg ctcgcgttcg ttcgctgagg cgctcgagcg ccgtggcggc gagctctggt
     4981 cggttgccac gcaccgccgc ctcgatgagc tcgaccagcg ccagggcgta gagggagagg
     5041 tcatcctgcg cgcatgcccg ctgcgccggg gcgagcgcct cgtcgtagtg gccgaggccg
     5101 ttgaagagca ccgcatttgc gcactcgatc atgctcagcg ccatgccctg gcctctggcg
     5161 gtcgcgtccg ggcggcccgc ttcgatcagc tccagcgctt gggcctcgtt gccgcgccac
     5221 gcggcgacca tctgcctagc ctgtaccagg agcgcggtat gggtcgcctg ggcgaccgcg
     5281 tatgcctcct ccagcagcgc cgacgcgtcg gcgtactccc cggcgtgcac gtgcacgccg
     5341 gcgtggtagc tggccgatat gggaaagacg ctgagcgcgc ctgcctcgcg cgcacggcgc
     5401 acgccgctgg tagccagctc gtcccagatc tcgtgctccc agagatccgc ggcgacgcgg
     5461 cacgccagcc agagccagcg gtcgttggcg ggactggccc cgtcgtctcg tcggaaggcc
     5521 tcgagcgccg cccgcagcgg agccacggct gccgcgtaac cctgcgtgac cagcgtcgct
     5581 aggccattca ggagcaagtc ggatgccgct gggggctgcg ccgggggtac ggcgcggacc
     5641 acccgcagca cgtcccgacc gctgccgagg cgaccggcga agatcgccgc ggcaagcgct
     5701 tcgaggcacg tctcacgcgc caggctcgga tcgagggcga ctaggcgctt ggcggcgtcg
     5761 agcagca
//
LOCUS       DASVEU010000245        12594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1343789,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000245 DASVEU010000000
VERSION     DASVEU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343789"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /gene="lepA"
                     /locus_tag="VF225_10380"
     CDS             <1..1452
                     /gene="lepA"
                     /locus_tag="VF225_10380"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7145700.1"
                     /translation="YLAIENNLDIVPVVNKIDLPSADPDAVAREIADLIGDDPEHVLR
                     ISAKTGQGVESVLDAIVERVPAPSGDPDAPARALIFDSSYDQYRGVVAFVRVVDGSFS
                     TREPLRAMALETRFEAEELGFFSPTRSAASTLEAGEVGYVITGLKDVSRLRVGDTITS
                     ERRPAAAPLPGYKEVKPMVFSGLFPTDSDAYPELRDALERLKLNDAALFYEPETSQAL
                     GFGFRCGFLGLLHMEIVRERLEREFDLDLIVTAPNVAYRVKPPNGEWKEVHTPSDMPN
                     EVEEVEEPYIKASIIVPKEYVGAVMELNNDRRGTFDHMEYLSEQRVHLTYELPLAEIV
                     LDYYDQLKSRTRGYASFDYDVIGFRPGKLVRVDVLVGGESVDALSLVIHRDSAYDRGR
                     ALVERLREEIPRQHFDVAIQAAIGARVIARETVKARRKDVLAKCYGGDISRKRKLLEK
                     QKAGKKRMKQVGMVEVPQEAFLAVLNLSDEKKS"
     gene            1449..2558
                     /gene="hemW"
                     /locus_tag="VF225_10385"
     CDS             1449..2558
                     /gene="hemW"
                     /locus_tag="VF225_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7145701.1"
                     /translation="MSLGVRHLYVHLPFCAHRCGYCDFVTVVGRHSQHGEYVEALLAE
                     LELERGVLAEQLETVFLGGGTPTFMEPAALERLLASLPQASEVTVEANPETVTQELAR
                     MLRAQGVNRISLGVQSFRPHLLEVLDRVVQPDTVRRAFYHLRDAGFDNISFDLIYGIP
                     GQAPSDLDADLADALELGPEHLSCYELEAKHGTRFTHAHGEELERQADAMESYFELVV
                     ERLTGAGYRWYETANFCRASERDLRSRHNLAYWLGRDYLALGVGAVSTIGGRRWRNTP
                     RLAGYMQALEREIAPEREIEPLSHDVQRRERVMLGLRLDEPLVLGDLAAVVDERAVSR
                     LEVLGLVRSDAERQTLALTPRGRFLGGGVTAELLV"
     gene            2581..3624
                     /gene="hrcA"
                     /locus_tag="VF225_10390"
     CDS             2581..3624
                     /gene="hrcA"
                     /locus_tag="VF225_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7145702.1"
                     /translation="MASDPAATLSVRQRALLALVIEEYVATGQPVGSRHLVERGELNV
                     SPSTVRNDLSELEARGLLTHPHTSAGRVPTEQGYRLHADELLAQQDPRPGSFPLDLSE
                     LRREIDSALEATTEMLSRVTSQLALVSAPPLETTTVRHVEVLLLNPRTVMAVVITSTG
                     GVAKRVVTFDHAVDPGLAKWAGEYLNERVAGLQLGTSRLRRELEDSSLSGAEREFLAA
                     LRPAFTDLLQAEQRLYVGGAAGLLDSGREDDLGIYRHVLVTLERRAALLEILGEALDP
                     RRAFVRMGGDLEHPAFQDAALVGATYGLTTRALGAVGLLGPARMDYDKAIRSVRGAAQ
                     ELSRFVEDVYEDN"
     gene            3651..4766
                     /gene="dnaJ"
                     /locus_tag="VF225_10395"
     CDS             3651..4766
                     /gene="dnaJ"
                     /locus_tag="VF225_10395"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7145703.1"
                     /translation="MATTERDYYELLGVSRTANAEEIKRAFRGLARELHPDVSKEPDA
                     DHRFREVVEAYEVLSSSERRELYDRFGHAGLRSGGFQPTAFDLGSLSDLFSAFFGDDL
                     FGGGGRGRARGADIAARVEIDLAEVATGTKRDVPFGVAVPCKRCGGNGAEPETEIRSC
                     PTCGGNGRVRQVSRSVFGEFVRAGTCPTCNGSGQLIEQPCAECHGAGRVLEERNIEVE
                     IPPGIHDGQRIRLSGEGHSGTPGSRAGDAYIEVHVRHDERFVREGNDVYTTVDLTFTQ
                     AALGATVSVPTLGGDEELTFEPGTQPGDVRVLRGRGLPVLQGFGRGNQRVFVRVAVPR
                     QLTEEQRRLLEEFDGVSTDATDEAGEGFFDKLKSAFR"
     gene            4829..5524
                     /locus_tag="VF225_10400"
     CDS             4829..5524
                     /locus_tag="VF225_10400"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7145704.1"
                     /translation="MLELFPQGFEEVDSPDGIEFVAYTDASGEERLWHVFGGVRGQDI
                     AADWRDRWKAFHQPVRVGPLWIGPPWDDAPADAIAVVIDPGRAFGTGAHPTTQLCVEL
                     MLELEPASVVDFGCGSGVLSIVAAKLGFAPVIALDADENAVAATIANAGANDVQVDAR
                     RSNVLDEEAPAAAVALANITRASVEALAPRLHSRTFLTSGYLPTDDAPLPGFRHVRRI
                     TRAGWAADHYEAT"
     gene            5559..6755
                     /locus_tag="VF225_10405"
     CDS             5559..6755
                     /locus_tag="VF225_10405"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX7145705.1"
                     /translation="MTTFSVRFLGCKVSHTDAQEVRERLLFDGHLEQDMDSGADVAVV
                     NTCCVTHEAVRKSRQAAKRAARTHGRVYVTGCAANLAGDAFSSLPPNVTVVARPSEET
                     PAFVAGDVGAIGCVRADARLDRVRAFVKIQDGCSFSCNFCVIPLVRGGSRSRSAAAVL
                     DEVRRRVAHGHKEIVLTGINLGCYRDRTAGYALPRLVREVGAVPGLERLRLSSIEINH
                     VDDELVSALRDTAKVSRHLHVPLQSGDDGVLRDMARRYSAATYLRRLEPLADFNLTTD
                     AIVGFPTETECAFQNTLSVVEAAGITKVHVFPYSPRPGTVTAGDDTVPPDVKKDRGAR
                     LRAASDAACLARWREKIGADDVVLVDRPGRGYGDDYSPWLVAAPVGELVRVRGASVSE
                     EGILAA"
     gene            6748..7077
                     /locus_tag="VF225_10410"
     CDS             6748..7077
                     /locus_tag="VF225_10410"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7145706.1"
                     /translation="MPEDCLFCKLVREGDHVAKADGFVAIRDIYPRADTHLLVLPERH
                     VPSFREIGEFTPDETKRMLEFVAETAREAGLDDYRVVVNVGEGGGQTIFHLHWHILGG
                     QLKGPLA"
     gene            7265..7966
                     /locus_tag="VF225_10415"
     CDS             7265..7966
                     /locus_tag="VF225_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145707.1"
                     /translation="MSLLSLHNVEARYGAVKALHGVSLEVGDGELVAVLGANGAGKTT
                     TLRAISGTVRSSGDVVFAGKKLSRRPEATARVGIAHVPEGRGTFGELSVWENLRLGAY
                     ARRANVKEEARRVYKFFPRLEDQHDQQAGTLSGGEQQMLALGRAMMARPKLLLLDEPS
                     LGLAPLVVNQIFEALALMKRDDGMSVLVVEQNANLALEYAARAYLLEVGKVVVAGTSD
                     ELRANESVRKSYLGY"
     gene            7966..8868
                     /locus_tag="VF225_10420"
     CDS             7966..8868
                     /locus_tag="VF225_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7145708.1"
                     /translation="MFLGAFTWTEFAQQLAAGIREGAIYAGLALAIVIIYRSTRVINF
                     AQGEMATFTTFIAWSLMNHGLSFWTAFPIVLAIAFAGGVAIERVVIRPVENAPVLTIV
                     IITLGLALLLNGLMSVIWGGANRQFNGPFSTRTIDLGSVPISVQDIGIVAVSVGLVVL
                     LALFFRFTKLGLALRAAAVNPESSRLVGIRVGWMLALGWGIAAVLGAVAGMMIAPVVF
                     LDPNMMQTILLYAFAAAVLGGLDSPIGAVVGGILLGVTITLLGRYVGFIGSTLKLPAA
                     LLLILVLLLVRPGGLFGRVAVRRV"
     gene            8865..9887
                     /locus_tag="VF225_10425"
     CDS             8865..9887
                     /locus_tag="VF225_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7145709.1"
                     /translation="MRARLLLELAGFALLTLIVAALPRFVSDFRARELAIVGMYFIAL
                     LGLSILTGYSGQISLGHGAFMAIGGYTTAVLSVDGVYGHQIRDLWTIPIAGVIAGIAG
                     LLVGVPALRLSGLYLALITFGIAVSFPQLPKKFDHFLGGTTGKILNLAKPPFGLHTTT
                     NNWIYYLTWGIALVLLLAAWWLVRGKTGRALKAIRDSEVAAASSGISLARYKTLAFGI
                     SAFYAGVAGSLYAIANAYVNPDVFPIILSVYLVVGLAVGGLDSLVGLIAGAGLIYFLQ
                     NHADTVARWLNHLPALNLDPKQPGIPSMIFGAVLIVVMLLLPTGAGGLVRRLFGPLTS
                     RLYTRS"
     gene            9888..11108
                     /locus_tag="VF225_10430"
     CDS             9888..11108
                     /locus_tag="VF225_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145710.1"
                     /translation="MGVGARVLFVCGVVAALAVSSAGARPVVTPGVSANEIKLGSSVP
                     LSGEAAIAGNVARGIEAYFKYVNDKGGVFGRKLTFTYLDDGYDPGRAVNNTIRLIQQE
                     QVLAVFSSLGTSNNLAVRKLLNDAKVPQLYVSSGATTFGRDYKKYPWTIGYIPPYSEE
                     GELYARYVVKNIKQPKIAVLYQNDDYGRDLLGGLKRGLGSKTKSIVASVGYDPTSADV
                     QPQVTQLKASKANVLMVFAFGKFSLQAFNAVSRLGWKPQIFVNDVSSASALMAIVPQN
                     AANGSISIVFGKDPASPLWRNDKGIKLFQSILKKYGSSINARDLKDGYFTAGMATAYT
                     MVDTLKKAGRNPTRQSVMNAATHLNEKGNPFVLPGIVLHTTPSSRFPLTQVRLQRWSG
                     NAWHPFGKLISAKP"
     gene            complement(11109..>12594)
                     /locus_tag="VF225_10435"
     CDS             complement(11109..>12594)
                     /locus_tag="VF225_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145711.1"
                     /translation="YQRTLFIEHGFLLWNNFWYAGRYSFVNYSVLYYPLAAAIGIKLL
                     AVLTVAVAVVAFAEVVRHEWESHSRWAIRVFALVWALLVVSAAYPFMLGMTLALLALW
                     SLQQDRRWVFAGFTVLTLAASPLAFLLLVVLLAGVALARRGEHRRFVAPAAAIGAIAA
                     FEFLLRRMFPGRGTFPFSFEEFLAAGTFCVTGVVLTWKIAAARMLRWTYVVYFVACTA
                     AFAISSPVGENIARLRFVAAPIAILTLSLRRWQPRFFCAVALVLALSWNLTPLAASFI
                     HGVNDPAAHAAYWSPTIDYLKRNLRPSYRVEAVDTAGHWPADFLARARIPLARGWFRQ
                     EDFPQNRVLYGELGAKAYVAWLRRVSVRYVVLTSAPPDYSARAEERLLRSGRSGLAVV
                     YRTPTTTIFKVPSPRPLISAPAHVLALGYSSIKLAVPVEGKYRLNVTYAPYWHTRAGC
                     LTRTPDGMTELTVRRTGIVKLHFAVTATRALEAIAGTQPDTCSS"
BASE COUNT         2056 a         4152 c         4238 g         2148 t
ORIGIN      
        1 tacctcgcga tcgagaacaa cctcgacatc gttccggtcg tcaacaagat cgatctgccg
       61 tccgccgatc ccgacgcggt cgcacgcgag attgccgatc tgatcggcga cgaccccgag
      121 cacgtcctgc ggatctccgc caagacgggc cagggcgtcg agtccgtact ggacgcgatc
      181 gtcgagcgcg tgccggctcc ttcgggcgat cccgatgcgc ccgcgcgcgc actgatcttc
      241 gactcgtcct atgaccagta ccgcggtgtg gtcgcattcg tacgcgtcgt cgacggaagc
      301 ttctcgacgc gcgagccgct gcgcgcaatg gcgctcgaga caaggttcga ggccgaggag
      361 ctcggcttct tttcgccgac ccgcagcgca gcgagcactc tcgaggcggg cgaggtgggt
      421 tacgtcatca ccggcctgaa ggacgtctcg cgtctgcgtg tgggagacac gatcacgtcg
      481 gagcggcggc cggccgccgc tccgctgccc ggctacaagg aagtcaagcc gatggtgttc
      541 tccggcctct tcccgacgga ctcggatgct tatccggaat tgcgggacgc gctcgagcgg
      601 ctgaagctga acgacgcggc gctgttctac gagcccgaga cctctcaggc gctcggcttc
      661 ggctttcgtt gcggctttct cgggctgttg cacatggaga tcgtccgcga gcggctcgag
      721 cgcgagttcg acctcgacct gatcgtcacc gctccaaacg tcgcctatcg ggtgaagccg
      781 ccgaacgggg agtggaagga ggtgcacacg ccgtcggaca tgccgaacga ggtggaggag
      841 gtggaggagc cttacatcaa ggcctcgatc atcgtcccca aggagtacgt cggcgcggtc
      901 atggagctga acaacgacag gcgcgggacc ttcgatcaca tggagtacct ctccgagcag
      961 cgtgtccatc tgacctatga gctcccgctg gcggagatcg tgctcgacta ctacgaccag
     1021 ctgaagtcgc gcacgcgcgg gtacgcgagc ttcgactacg acgtgatcgg cttccgtccg
     1081 ggcaagctcg tgcgcgtcga tgtgctcgtc ggcggcgagt ccgtcgatgc gctctcgctc
     1141 gtcatccacc gcgactcggc ctacgaccgc gggcgcgcgc tcgtcgagcg actgcgcgag
     1201 gagattccgc gccagcactt cgacgttgcg atccaggcgg caatcggggc tcgcgtgatc
     1261 gcgcgcgaga ccgtgaaggc caggcgcaag gacgtgctcg ccaagtgcta cggcggcgac
     1321 atctcgcgca agcggaagct gctggagaag cagaaggccg ggaagaagcg catgaagcag
     1381 gtcggaatgg tcgaggtgcc gcaggaggcg ttcctcgcgg tgctcaatct gagcgacgag
     1441 aagaagtcgt gagcctcggc gtgcggcatc tttacgtgca ccttccgttc tgtgcgcacc
     1501 gctgcggtta ctgcgacttc gtgacggtgg tcgggcggca ctcgcagcac ggggagtacg
     1561 tcgaggcgct gctcgccgag ctcgagctcg agcgcggggt gctcgcggag cagctggaga
     1621 cggtctttct cggtgggggc acgccgacct tcatggagcc tgctgcgctg gagcgcctgc
     1681 tcgcgtcgct gccgcaggca agcgaggtga cggtcgaggc gaatcccgag accgtgacgc
     1741 aggagctcgc ccgaatgttg cgcgcgcagg gtgtgaatcg aatctcgctc ggcgtgcaga
     1801 gcttccggcc gcacctgttg gaagtcctcg accgtgttgt ccaaccggac accgtccggc
     1861 gagccttcta ccatcttcgt gatgccggat ttgacaacat ctcgttcgac ctcatctacg
     1921 ggattccggg ccaggccccc tccgatctcg atgccgacct cgccgacgcc ctcgagctcg
     1981 ggcccgagca cctgtcgtgt tacgagctcg aggcgaagca cgggacgcgc ttcacccacg
     2041 cgcatggcga ggagctcgag cgtcaggcgg atgcgatgga gtcgtacttc gaactggtcg
     2101 tcgagcggct gaccggcgcc ggctatcgct ggtacgaaac cgctaacttc tgccgcgctt
     2161 ccgagcgtga tctgcgctct cgacacaacc tcgcctactg gctcggccgc gactacctcg
     2221 cgctcggcgt cggcgcggtc tcgacgatcg gcgggcgccg ttggcggaac acgcctcgcc
     2281 tggccgggta catgcaggca ctggagcgtg agatcgcacc tgaacgcgag atcgagcctc
     2341 tctcgcacga cgtgcaacgc cgtgaacgcg tgatgctcgg cctgcgcctg gacgagccac
     2401 tcgtcctcgg cgacctcgcc gcggtcgtcg acgagcgtgc cgtttcgcgg ctggaggtcc
     2461 tcgggctcgt gcgcagcgat gccgagcggc agacgcttgc gctcactcca cgcggccgat
     2521 tcctcggcgg tggagtcact gctgagctgc tcgtgtgaag cgccgctaaa attcctgcaa
     2581 atggcctctg atccagcggc aaccctctcg gtgcgacagc gcgcgctgct cgcgctcgtc
     2641 atcgaggagt acgtcgccac cggccagccc gtcggctcga ggcacctggt ggagcgcggg
     2701 gagctgaacg tttcgccgtc gaccgtgcgc aacgacctct ccgagctcga ggcgcggggt
     2761 ctgctcacgc acccccacac gtcggccggc cgggttccga ccgaacaggg ctaccggctc
     2821 cacgcggacg agctgctggc gcaacaggat ccgcgccccg gctcgtttcc gctggacctc
     2881 tccgagctgc gccgcgagat cgactcggcc ctcgaggcga cgacagagat gctctcgcgt
     2941 gtaactagcc agctcgcgct ggtgtccgcg ccgcccctcg agacgacgac ggtgcgccac
     3001 gtcgaggttc tgcttctcaa tccacgtacg gtgatggccg tcgtcatcac ctcgaccggc
     3061 ggggttgcca agcgggtcgt cacgttcgac cacgccgtcg acccggggct cgccaagtgg
     3121 gcgggggaat acctgaacga gcgggtggcc ggccttcagc tcgggacgag ccggctacgc
     3181 cgagagctcg aggactcgag cctctcggga gccgagcgcg agttcctcgc agcgttgcga
     3241 cctgctttca cggatttgct ccaggccgag cagcgactct acgtgggcgg cgcggcgggc
     3301 ctgctcgact cggggcgcga ggacgacctc ggcatctacc ggcacgtgct ggtgacgctg
     3361 gagcgtcgcg ccgcgctgct cgagattctc ggcgaggcgc tcgacccgcg ccgcgcgttc
     3421 gtccggatgg ggggcgacct cgaacacccg gccttccagg acgcggcact cgtgggggcg
     3481 acctacggac tcacgacccg cgcgctcggg gcggtcggct tgctcgggcc ggctcgcatg
     3541 gactacgaca aggcgatccg ttcggttcga ggtgcggcgc aggagctttc ccgcttcgtc
     3601 gaggacgtct acgaggacaa ctgaggccgt cgctacgcta agtctcggcg atggcgacga
     3661 ctgagcgcga ctactacgag cttctcggcg tgtcgcgaac ggcgaacgcc gaggagatca
     3721 agcgcgcgtt ccggggcctc gcccgcgagt tgcatcccga cgtgtcgaag gagccggatg
     3781 ccgatcaccg gttccgcgag gtcgtcgagg cgtacgaggt tctctccagc tccgagcggc
     3841 gggagctcta cgaccgcttc ggtcatgccg gtttgcgcag cggcggcttc cagccgaccg
     3901 cgttcgacct cggcagcctc tcggatctct tctccgcatt cttcggcgac gacctgttcg
     3961 gcgggggagg acgggggcgt gcgcgcggcg ccgacatcgc cgcaagggtc gagatcgacc
     4021 tggcggaggt ggcgacggga accaagcgcg acgtgccatt cggcgtcgcg gttccgtgca
     4081 agcgctgcgg aggaaacggc gccgagccgg agacggagat ccgctcctgc ccgacgtgtg
     4141 ggggcaacgg ccgcgtgcgt caggtctcgc gcagcgtctt cggggagttc gtgcgcgccg
     4201 ggacatgtcc gacgtgcaac ggctccgggc agctgatcga gcagccgtgc gcggaatgcc
     4261 acggcgcagg ccgcgtgctc gaggagcgca acatcgaggt cgagataccc ccgggcatcc
     4321 acgacgggca gcgaatcagg ctgagtggcg aaggtcattc aggaacgccg ggaagccgcg
     4381 ccggcgacgc gtacatcgag gtgcacgttc gccacgacga acgtttcgtc cgcgaaggca
     4441 acgacgtcta caccaccgtc gacctgacgt tcacgcaggc cgcgctcggc gccacggtgt
     4501 cagtgccgac gctcggcggc gacgaagagt tgacgttcga gcccgggacc cagcccggcg
     4561 acgtgcgagt cctgcgcgga cgcgggctgc cggttctgca ggggttcggt cgcggcaatc
     4621 agcgcgtgtt cgttcgcgtc gccgtgccgc gccagctgac agaggagcag cgccggctgc
     4681 tggaggagtt cgacggtgtg tcgacggacg cgactgacga ggccggcgag gggttcttcg
     4741 acaagctgaa gagcgcattc cgctgaacgc gatccggcgg gtctcggttg cggtcccctt
     4801 cgagcgcgca gaggaagcgc gcgcacagat gctcgagctg ttccctcagg gcttcgaaga
     4861 ggtcgacagc cccgacggga tcgagttcgt cgcctatacc gatgccagcg gcgaggagcg
     4921 tctgtggcac gtcttcggag gcgtgcgagg ccaggacatt gctgccgatt ggcgcgaccg
     4981 ttggaaagcg tttcaccagc ccgtgcgtgt cggaccgctc tggatcggcc cgccgtggga
     5041 cgacgcgccc gcagacgcga ttgcggtggt gatcgacccg ggtcgtgcct tcggcaccgg
     5101 cgcccatccg acaacacagc tgtgcgtcga gttgatgctc gagctcgagc cggcgagcgt
     5161 cgtcgatttc ggctgcggct ccggtgtgtt gtcgatcgtc gctgcgaagc tcgggtttgc
     5221 gccggtgatc gcactcgatg ccgacgagaa tgctgtcgcc gcaacgatcg ccaatgccgg
     5281 ggcgaacgac gtccaggtcg acgcacgccg gtcgaacgtg ctcgacgaag aggctccggc
     5341 tgcagcggtt gcgctggcga acatcacgcg cgcgtcggtg gaggcgctcg cacctcgttt
     5401 gcattccagg actttcttga cctcgggcta ccttccgacc gacgacgcgc cgctccccgg
     5461 cttcaggcat gtgcggcgca tcacgcgcgc cggttgggct gcggaccatt acgaggcgac
     5521 gtgagagccg ggctcttggg ccagttagca tcagagccgt gacgacgttc tcggtccgtt
     5581 ttctcggctg caaggtctcg catacggatg cgcaagaagt ccgcgagcgc ctgctgttcg
     5641 acggtcatct cgaacaagac atggatagtg gtgccgacgt cgcggtcgtc aacacgtgct
     5701 gcgtcacgca cgaggccgtc cggaagtcgc gccaggcggc caagcgcgcg gcgcgcacgc
     5761 acggtcgcgt ttacgtgacc ggctgcgccg cgaatctcgc cggcgacgct ttctcgagcc
     5821 tgccaccgaa cgtgaccgtg gtcgccaggc cgagcgagga gacaccggcc ttcgtggccg
     5881 gagacgtggg cgcgatcggg tgcgttcggg cagatgcccg tctcgaccgc gtgcgcgcgt
     5941 tcgtgaagat ccaggacggc tgcagcttct cctgcaactt ctgcgtgatt ccgcttgtcc
     6001 gtggtggatc gcggagccgc tccgcggccg cggtgctcga tgaagttcga cgacgcgtcg
     6061 cgcacggaca caaggagatc gtccttaccg ggatcaacct cggctgctac cgggaccgca
     6121 cggccggcta cgccctgccg cgactcgtgc gcgaggtggg cgccgttccc ggtctcgagc
     6181 ggttgcgctt gtcgtcgatc gagatcaacc atgtcgacga cgagctcgtg tcagccctgc
     6241 gggatacggc gaaggtcagc cggcatctgc acgtcccgct ccaatcgggc gacgacgggg
     6301 tgctacggga catggcccgc cgctacagcg ctgccactta cctccgccgt ctcgagccgc
     6361 tcgccgactt caacctgacg acggatgcaa tcgtcggttt tccgaccgag acggagtgcg
     6421 cgtttcagaa cacgctctcg gtcgtcgagg cagccggcat cacaaaagtc cacgtctttc
     6481 cgtactcgcc ccgacccgga acggtcaccg cgggagacga caccgtcccg cccgacgtga
     6541 agaaggatcg gggcgccagg ctgcgcgcag cgtcggatgc ggcgtgtctc gcgcgctggc
     6601 gtgagaagat cggcgcagat gacgtcgtcc tcgtcgatcg ccccggccgc ggctacgggg
     6661 acgactactc gccgtggctc gtcgctgcac ccgtcggcga gctcgtccgc gtccgcggag
     6721 cgagcgtctc cgaggaggga attctcgctg cctgaggact gcctcttctg caagctcgtc
     6781 cgagagggcg accatgttgc gaaggcggac gggttcgtcg cgatccgtga catctatccg
     6841 agggccgaca cgcacctgct cgtcctgccc gagcggcatg tcccgagctt ccgcgagatc
     6901 ggcgaattca cgcctgacga gacgaagcgg atgctggagt tcgtggcaga gaccgcgcgc
     6961 gaggcaggac tcgacgacta tcgcgtcgtc gtgaacgtgg gcgagggcgg cggtcagacg
     7021 atcttccatc tccactggca catcctcggc ggtcaactca aaggaccact ggcatgagcg
     7081 tcgctcgctt tcagctcact cccgtgaggg aaaccatgtt tccctcacga gctcccttct
     7141 ttagggatcg cctcagcgcc cgcagcgtcg cgaccgccgc cgcgaacgct gaggagaaag
     7201 agcggggaaa cctcccggtt tcccctttcg ccccttccac cgctcatcgg ccagagagcg
     7261 gccgatgagc ctcctctctc tgcacaacgt cgaagcgcgc tacggcgcgg tcaaggcact
     7321 gcacggcgtg tcgctcgagg ttggcgacgg cgagttggtc gccgtgctcg gggcgaacgg
     7381 cgccggaaag acgaccacgc tgcgcgcgat ctccggcacc gtgcgcagca gcggcgacgt
     7441 cgtgttcgcc gggaagaagt tgtcgcgtcg tccggaagcg acggcgcgcg tgggaatcgc
     7501 gcacgttccc gagggtcgcg gcaccttcgg cgagctctcg gtgtgggaga acctgcggct
     7561 cggcgcctac gcgcgacgcg ccaacgtcaa ggaggaggcg cggcgtgtct acaagttctt
     7621 cccgcggctg gaagaccaac acgatcagca ggcgggaaca ctctccggcg gcgagcagca
     7681 gatgctggca ctcggccgcg cgatgatggc caggccgaag ctgctcttgc tcgacgagcc
     7741 ctcgctcggc ctcgctccgc tcgtggtcaa tcagatcttc gaagcgctcg cgctcatgaa
     7801 gcgcgacgac ggaatgtcgg tcctcgtcgt cgagcagaac gcgaacctgg cgctcgaata
     7861 cgccgcccgc gcgtacctct tggaggtcgg caaggtcgtc gtggcaggaa cgagcgacga
     7921 gctgcgcgcc aacgaatccg tccgcaagtc ctatctgggg tactgatgtt tctgggcgcc
     7981 ttcacctgga cggagttcgc gcaacagctg gcagcaggca tccgcgaggg cgcgatctac
     8041 gcagggctcg cgcttgcgat cgtgatcatc taccgctcca cgcgcgtcat caacttcgcg
     8101 cagggtgaga tggcaacgtt cacgacgttc atcgcctggt cgttgatgaa tcacggcctc
     8161 tcgttctgga ccgcctttcc gatcgtgctc gcgatcgcgt tcgccggtgg tgttgcgatc
     8221 gagcgcgtcg tgatccgccc ggtcgagaat gcgcccgtcc tgaccatcgt gatcatcacc
     8281 ttgggcctcg ctctgctgct caacggcttg atgagcgtga tctggggcgg cgccaacagg
     8341 cagttcaacg ggccgttctc cacgcggacg atcgacctcg gcagcgtgcc gatcagcgtc
     8401 caggacatcg gaatcgtggc ggtgtccgtg ggactcgtcg tcctgctcgc cctgttcttc
     8461 cgcttcacga agctcggact ggcgctgcgt gccgcggcgg tgaaccccga gtcgtcgcgg
     8521 ctcgtcggca ttcgcgtggg ctggatgctc gcgctcggtt ggggaatcgc cgcggtactc
     8581 ggggcggtcg cgggaatgat gatcgcccct gtcgtcttcc tcgatccgaa catgatgcag
     8641 acgatcctgc tgtacgcgtt cgcggccgcc gtgctcggag ggctggacag tccgatcgga
     8701 gcggtggtcg gcgggatcct cctcggcgtc acgatcacgc tgctcggccg ctacgtcggc
     8761 ttcatcggct cgaccttgaa gctgccggcg gcgctgctgc tcattctcgt tctcctgctc
     8821 gtgcgtccgg gcggcctgtt cggccgggtt gcggtgagac gcgtatgaga gccagactct
     8881 tgctcgagct cgccggtttc gcattgctca ctctgatcgt cgcggcgctg ccgcgcttcg
     8941 tgagcgactt ccgggcccgc gagctggcga tcgtcgggat gtacttcatc gcgctgctcg
     9001 gcctgtcgat cctcaccggg tacagcggcc agatctcgct cggccacggt gccttcatgg
     9061 cgatcggcgg ctacacaacg gcggtgctca gcgtcgacgg ggtgtacggc catcagatcc
     9121 gcgatctctg gacgattccc atcgccggcg tgatcgccgg catcgccgga ctgctcgtcg
     9181 gcgtaccggc gctgcgcctc tccggcctct atctcgcgct gatcaccttc gggattgcgg
     9241 tgtcgtttcc acagttgccg aagaagttcg atcacttcct cggcggcacg accggcaaga
     9301 tcctcaacct cgccaagccg cccttcgggc tacacaccac gacgaacaac tggatctatt
     9361 acctgacctg gggcattgca ctcgtgctgc tgttggcggc ctggtggctc gttcgcggga
     9421 agaccgggcg cgcgctgaag gccattcgcg acagcgaggt ggccgccgca tcgtcgggga
     9481 tcagccttgc gaggtacaag acgctcgcgt tcggaatctc cgcgttctac gcaggagtcg
     9541 cgggatcgct gtatgcgatc gcgaacgcct acgtcaatcc ggacgtcttt cctatcatcc
     9601 tgtcggtcta cctcgtcgtc ggactggcgg tgggcggcct cgactccctg gttggtctca
     9661 tcgcgggcgc cgggctcatc tacttcctgc agaaccacgc ggacaccgtg gcgcgctggc
     9721 tcaaccacct gccggcgctg aatctcgatc cgaagcagcc cggtatcccg agcatgatct
     9781 tcggcgccgt cctgatcgtc gtcatgctcc tgcttcccac cggcgcgggc ggcttggtcc
     9841 ggcgtctctt tgggccgcta acaagtcggc tatacactcg gtcctaggtg ggagtgggcg
     9901 cgagggttct ctttgtgtgc ggcgttgtcg ccgcgctcgc agtctcatcg gccggtgcgc
     9961 gcccggtcgt cactcccggt gtgtcggcaa acgagatcaa gctcggctcg agtgtccctc
    10021 tgagcggcga ggcggcaatc gccggcaacg tcgcgcgtgg catcgaggcg tacttcaagt
    10081 acgtcaacga caagggcggc gtgttcggcc gcaagctcac cttcacgtat ctcgacgacg
    10141 gctacgaccc cgggcgcgcg gtcaacaaca ccatccggct cattcagcag gagcaggtcc
    10201 tcgccgtgtt cagctcactc gggacgagca acaacctggc cgtgcgcaaa ctgctgaacg
    10261 acgccaaggt gccgcagctc tatgtctcct cgggcgcgac caccttcggc cgcgactaca
    10321 agaagtaccc gtggacgatc ggttacatcc cgccttactc cgaggagggt gagctctacg
    10381 ctcgctacgt cgtcaagaac atcaagcagc cgaagatcgc cgtgctgtat cagaacgacg
    10441 actacgggcg cgacctgctt ggcggtctga agcgcgggct cggctcgaaa acgaagtcga
    10501 tcgttgcgag cgtcggctac gacccgacct cggccgacgt ccagccgcag gtgacgcaac
    10561 tgaaagcctc gaaagccaac gtcctgatgg ttttcgcctt cggaaagttt tcgctgcaag
    10621 ccttcaacgc agtctcgcgg ctcggatgga agccgcagat cttcgtcaac gacgtgtcgt
    10681 cggcgtccgc gttgatggcg atcgtcccgc agaacgctgc gaacggctcg atctcgatcg
    10741 tcttcggcaa ggatcccgcg tcgccgctct ggcgtaacga caagggcatc aagctcttcc
    10801 agtcgatcct caagaagtac gggtcgagca tcaacgcacg ggacctcaag gacggctact
    10861 tcacggccgg aatggcgacg gcctacacga tggtcgacac gctcaagaaa gccggcagga
    10921 accccactcg ccagagcgtg atgaacgcgg ccactcacct gaacgagaag ggcaatccct
    10981 tcgtgctgcc gggcatcgtc ctccacacga ccccgtcgag ccggttcccg ctcacgcagg
    11041 ttcgcctgca gcgctggtcg ggcaacgcct ggcatccgtt cggcaagctg atctcggcca
    11101 agccctagtc aagaactgca ggtgtcgggc tgggtgcccg caatcgcctc gagtgcgcgg
    11161 gtcgcggtca ccgcgaagtg gagctttacg atccctgtgc gccgcaccgt cagctccgtc
    11221 atgccgtcgg gagtacgcgt gaggcagccc gcccgcgtgt gccagtaggg cgcgtaagtc
    11281 acgttcagcc ggtacttgcc ctcgaccggt acggcgagct tgatcgacga gtaccccagc
    11341 gcgagaacgt gggccggcgc cgagatcagc ggacgtgggg agggaacctt gaagatcgtg
    11401 gtcgtcggcg tccggtacac gaccgccagt cccgagcggc cgcttctcag caaacgttcc
    11461 tctgctcgcg cgctgtagtc gggcggtgcc gacgtgagga cgacatagcg gacgctgacc
    11521 cgccgcagcc aggcgacgta ggccttggcc cccaactccc cgtacagcac gcgattctgc
    11581 gggaagtcct cctgtctgaa ccagccgcgc gcgagcggga tccgcgcccg ggcgaggaag
    11641 tcagcgggcc agtgtcccgc cgtgtcgacg gcctcgacgc ggtacgacgg acggaggttg
    11701 cgtttcaggt agtcgatcgt cggcgaccag tacgccgcgt gcgccgccgg gtcgttgacg
    11761 ccgtggatga agctggccgc gagcggggtc agattccacg agagcgctag aaccaaagcc
    11821 acggcgcaga agaagcgcgg ctgccaacga cggagcgata gcgtcaggat cgcgatcggt
    11881 gcagcgacga agcgcagccg cgcgatgttc tctcccaccg gtgacgagat cgcaaaagct
    11941 gcggtacacg cgacgaagta cacgacgtag gtccagcgaa gcatccgcgc ggccgcgatc
    12001 ttccaggtga gtaccacgcc cgtcacacag aaagtgcctg ccgcgaggaa ttcctcgaag
    12061 gaaaacggaa acgtgccgcg gcccgggaac atgcgccgga gcaggaactc gaacgccgcg
    12121 atcgcgccga tggccgcggc cggggcgacg aaacgcctgt gctcgccgcg ccgtgcgagc
    12181 gccactccgg ccagcaggac gacgagcaac aggaatgcga gcgggctggc ggccagcgtc
    12241 agaacggtga agcccgcaaa cacccagcgg cggtcctgtt gtagcgacca cagcgccaac
    12301 agtgcgaggg tcatcccgag catgaacgga tacgccgcgg agacgacgag cagagcccag
    12361 accagcgcga acactcgaat cgcccaccgg ctgtgcgact cccactcgtg gcggacgacc
    12421 tctgcgaaag cgaccactgc gacggccacc gtgagtacgg cgagaagctt gatgccgatc
    12481 gcggcggcca gtgggtagta caggacgctg tagttcacga agctgtagcg gcctgcgtac
    12541 cagaagttgt tccagagcag gaagccgtgc tcgatgaata gcgtgcgctg gtag
//
LOCUS       DASVEU010000246        11587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1537829,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000246 DASVEU010000000
VERSION     DASVEU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537829"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VF225_10440"
     CDS             complement(<1..729)
                     /locus_tag="VF225_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145712.1"
                     /translation="MKPTIVEPAAEPTEEDEMSTSVSPTTITDRELEQVERANATTAT
                     PVVFIHGLWLLPSSWANWADFFEQAGYAPLTPVWPDDPETVEEARANPEVFAKKTLRQ
                     IADHTTEIIDALDKKPAVIGHSTGGLLAQMLAGRGLSAATVAIDPGVFRGVLPLPASV
                     LKGVGPFLLNPLTRGRAITFTFDQFKYGWANRLDEKEARGLYDTFHVAGSGISLVQMG
                     NANLNPWTEAKVNTKNPDRGPLLII"
     gene            complement(726..1520)
                     /locus_tag="VF225_10445"
     CDS             complement(726..1520)
                     /locus_tag="VF225_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7145713.1"
                     /translation="MKIVVIGGTGLIGSKLVEKLREAGHDPLAASPDSGVDTLTGEGL
                     AEALEEAEVVVDVSNAPDWNDDAVMDFFLTSSRNLLAVETAAGVGHHIALSVVGADRL
                     ADSGYFRAKVAQEEVIKAATVPYTIVRATQFFEFIARIADSNTDGETVRMPPVLVQPE
                     AADDVAAALAVAAVGAPVNGTVELAGPERFRLDELTHRVLSANGDARRVTADGRARYF
                     GAELDDRSLVPGDDARIAPTRLEDWLDRTTLKHTGPRTPKPEGGSE"
     gene            complement(1540..2385)
                     /locus_tag="VF225_10450"
     CDS             complement(1540..2385)
                     /locus_tag="VF225_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145714.1"
                     /translation="MISEKPTRLSRRMIAMSSALVLVALVWIGGGGHAAATRQASSDP
                     RPTIVLVHGDWADASSWNGVIERLQDRGFTVVAPPNTLRGPSQDAPYLASYLQTVPGP
                     IVLVAHSYGGFVITNAATGNPNVKALVYIDAFVPDENETALDLVGGTTSCVTDDGAFN
                     PVPIDGGLDLYLRWEAVPPYAGFMECFANGVNVRTAAVLAAGQRPASAAQFEDPSGAP
                     AWKTIPSWALLGTLDNVIPPTLQEEMYERAGAQISTVKTGHLSLITRPDVVVRVILSA
                     AEATT"
     gene            2684..5428
                     /locus_tag="VF225_10455"
     CDS             2684..5428
                     /locus_tag="VF225_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7145715.1"
                     /translation="MSAQNTLRGRDAECAVLDVLLDAARRGESRTVVLRGEAGVGKTA
                     LLDYAIGSAQDFRVLRSVGVESEMELAFASLYLLCAPILDRRGALPGPQREALDVVFG
                     LDRGPAPDRFLVGLATLSLLAETANERPLLCVIDDAQWLDSASALAFAFVARRLLADP
                     IGLLFAVREPIDVRELAGLPELVVGGISEDDARMLLGSVLPEPLDERVRDRIVAESHG
                     NPLALLELPRGMPPGELAGGFALPDARPLADRIERSFLRQVRSLPPDSQRLLLTAAAE
                     PVGDVTLLWRAAEPLGVGPDAVAPAEAAGLIELGGRVRFRHPLVRSAIYRAAAPNERR
                     EIHRALATATDPVADPDRRAWHRAHSTVGLDEAVAGELERSADRARRRGGFAAMAAFL
                     ERATELTPDPARRGTRALAAAQAKLEAGAPEAAHALVATAELTPLDELQSARLQRLRA
                     QIAFARRRGSDAPPLLLDAAKRLVPLDPEVARETCLEALAAAIFAGRGVNGRDVLQVV
                     HAAPPASRPPGATDLLLNGLATRATEGYAAGVAPLREALEAFRRDDGDTPANNRWLWL
                     ACRVAADLWEHEIWEELATHGVRRARKTGALSVLPIAASYRAGAHVHAGEYAEASALL
                     EEAYAISQTTHTALLVQARQIVAAWRGNEAEALELIGAGRPEAAARGQGMALSMIECA
                     DALLCNGLGRYEGALLAAERACAQDELSLYALALVELVEAAVHSNKPKVAAAALERLS
                     ERTRASGTEWALGIEARSRALLTERTSAESLYEEAIERLAHGRLAPYLARAQLVYGEW
                     LRRENRRVDAREQLRAAHDTFSRIGAEGFAERSRRELSATGETVRKRTLDTRELLTAQ
                     EAQIAHLARDGLSNPEIGAQLFISPRTVQYHLGKVFAKLDVTSRNQLSRVPASRLTVS
                     "
     gene            5828..6640
                     /locus_tag="VF225_10460"
     CDS             5828..6640
                     /locus_tag="VF225_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145716.1"
                     /translation="MAEVPAKAKPDTIVLIHGFWVTPRSWEHWKEYYEAKGYGVLTPA
                     YPGFEVEVEALRADPTPIEQLQVPEIIDMLENMIGKLDRPPIIIGHSAGGVFTQILLD
                     HGFGAVGVAMNSAPTEGVKVVPLSEIRSGFPILKNPANRHRAVGFTPKQWHYVFANTF
                     SDEKSLELYERYHIPASGRVFWGSALANIHPGKDDTYVDYHNDARPPLLFISGKQDHL
                     FPPKVGHSNAKHYKSKTVTEIVEFDGPHLMPAIDGWEEVADYALDWALKHAE"
     gene            6722..6886
                     /locus_tag="VF225_10465"
     CDS             6722..6886
                     /locus_tag="VF225_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145717.1"
                     /translation="MNAAGVAEVIREALDSRGQSSNIKATGDRAAQFELATMADGMAR
                     VFEITVEERG"
     gene            7276..8499
                     /locus_tag="VF225_10470"
     CDS             7276..8499
                     /locus_tag="VF225_10470"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7145718.1"
                     /translation="MSEYSIDDVAARAGAAHDYVSRLVELGVVSVGEGGGFSEGDVRR
                     VRLMQTLERAGVPLEEVAAAIRRGELSLGFLDMSHFTRLSSLSGVTFQELSEKTNVPV
                     ELLLVIREAAGFAEAHPTDRARENELQIVPLIEAQVALGFRLPVIERWLRAYGDSLRR
                     IAETEGEWWHTEIELPLLESGMTAAEMMEAADGRVVHRIVPFLDQALVALYHGHQEHA
                     WTKNFLDGIEAALSGAGVHSRVERPPAICFLDIAGYTRLTEERGDEAAAELAGTMSRI
                     VQRTSTSHGGRPIKWLGDGVMFHFPEPGPGVIAALDMVEGIAGAGLPPAHVGLHAGPV
                     LFQEGDYFGRTVNIASRIADYARPGEVVVSQPVVDAAEATPVSFVEIGPVELKGLSES
                     IRLHTARRDARAPNG"
     gene            8560..9000
                     /locus_tag="VF225_10475"
     CDS             8560..9000
                     /locus_tag="VF225_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7145719.1"
                     /translation="MIEVRRDDEIHEEAGGWFDARWHFSFDHYRDSRQMGIGPLRVFN
                     DDRLAPAADWPMHPHADVEGLTYVVEGTFEHADSLGNGGTLLPGGVQRMTLGSGAYHS
                     ERNGSKTEPMRFLQFWILPDTPCCRRAWNSGSSRSMTVGTACCT"
     gene            complement(9306..9566)
                     /locus_tag="VF225_10480"
     CDS             complement(9306..9566)
                     /locus_tag="VF225_10480"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX7145720.1"
                     /translation="MSRVDGNAMAGMLGEIFVHEMTAARVACGGCGRIEAIGAEHAYM
                     DAPSVVLRCCHCEGVLLVLTRARDNYVLGLGKASWLEVAGSP"
     gene            complement(9579..10331)
                     /locus_tag="VF225_10485"
     CDS             complement(9579..10331)
                     /locus_tag="VF225_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX7145721.1"
                     /translation="MARAAVLGRLSWTLGEVVELIDETPRVRSIVLDVPDWPRHRAGQ
                     HVDVRLTAEDGYQAQRSYSIASAPEDPRLALTVERLPDGEVSSYLVGELRPGDMLELR
                     GPIGGYFVWEASRQEPLLLVAGGSGIVPLMSMLRHREASATVPGARLVYSSRSWEDVI
                     YREELERLSSAAGGPQVIHTLTRTQPPGWTGYARRVDGAMLAEAAWAPSADPMAFVCG
                     PTPFVESVASALVLIGYRPTSVKTERFGPTGG"
     gene            complement(10301..10921)
                     /locus_tag="VF225_10490"
     CDS             complement(10301..10921)
                     /locus_tag="VF225_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX7145722.1"
                     /translation="MGKWVMTYISRGFRGKRAEEREDSRLPPGQYLVDDFPVLSAGPT
                     PHTPLEEWTFSIEGDLGVLKSWSWEEFRALPSEEIRTDIHCVTKWSKLDTTWEGVSID
                     TLMEGVEHDATHAMAFSDGDYTANLPLEDLVDGKAWVVSAYDGQPLEAEHGGPARLLV
                     PHLYFWKSAKWVRGLRLMDHDEPGFWESYGYHIYGDPWREQRYWGD"
     gene            11141..11374
                     /locus_tag="VF225_10495"
     CDS             11141..11374
                     /locus_tag="VF225_10495"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7145723.1"
                     /translation="MRVFSTQSLVEGEIVKGRLEAEGIPVDLRGEREGPYPLGPAELF
                     VPSRFEAQARRVIEQIEQIESGSYEAPDGNGAE"
     gene            11343..>11587
                     /locus_tag="VF225_10500"
     CDS             11343..>11587
                     /locus_tag="VF225_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145724.1"
                     /translation="MRPLTGMVRSRSRRRPIGTGGIFRISGIGGHGRPRLELLHDANG
                     DRDDRYGEHHPGDDKEAPPPMTLTRITTGWRTSERRTP"
BASE COUNT         1904 a         3880 c         3956 g         1847 t
ORIGIN      
        1 gataatcagc aggggaccgc ggtcgggatt cttcgtgttc accttcgcct ctgtccacgg
       61 gttgaggttc gcgttgccca tctggacgag tgagatcccg gagccggcga cgtggaacgt
      121 gtcgtagagc cccctcgcct ccttctcgtc cagcctgttg gcccagccgt acttgaactg
      181 gtcgaacgtg aacgtgatcg cgcggccgcg cgtgagcggg ttgaggagga aggggccaac
      241 ccccttgagc accgaggccg gcagaggcag aacgccgcgg aagactcccg ggtcgatcgc
      301 caccgtggcc gcggagaggc cccggccggc gagcatctgc gccagcaagc ctccggtcga
      361 gtgcccgatg accgcgggct tcttgtccaa ggcatcgatg atctcggtgg tgtgatccgc
      421 gatctgcctc aaggtcttct tggcgaagac ctccgggttc gcccgggcct cttcgaccgt
      481 ttccggatcg tctggccaga ccggcgtcag cggcgcgtag ccggcctgct cgaagaagtc
      541 cgcccagttg gcccagctgc tcggtagcag ccagagcccg tggatgaaca ccactggtgt
      601 cgccgtggtc gcattcgcgc gctcgacctg ctcgagctcg cggtcggtga tcgttgtcgg
      661 ggaaacgctc gtgctcatct cgtcctcctc tgtcggctcg gcggccggct cgacgatcgt
      721 cggcttcatt cagagcctcc ttccggtttg ggcgttcgtg ggcccgtgtg cttgagcgtc
      781 gtccggtcaa gccagtcctc gagacgggtc ggagcgatcc gtgcgtcgtc acccgggacg
      841 agggagcggt cgtcgagctc tgcgccgaag tagcgcgcgc ggccatccgc cgtcactcgg
      901 cgtgcgtcgc cgttcgcgct cagcacgcgg tgggtgagct cgtccagacg gaaccgttcg
      961 gggcctgcca gctcgacggt gccgttcacc ggcgcgccca ccgccgcgac cgccaacgcg
     1021 gcggcgacgt cgtcggctgc ctcaggctga accagcacgg gcggcatgcg aaccgtctcg
     1081 ccgtcggtgt tggaatcggc gatgcgagcg atgaactcga agaactgggt cgcgcggacg
     1141 atcgtgtagg gcacggtcgc cgccttgatc acctcctcct gggcgacctt cgcccggaag
     1201 tacccgctgt ccgcgagtct gtcggctccc acgacggaca gggcgatgtg gtggcccacc
     1261 ccggccgcgg tctcgacggc gagcaggttg cgggacgagg tcaggaagaa gtccatcacc
     1321 gcgtcgtcgt tccaatccgg tgcgttcgac acgtcgacca cgacctcggc ctcttccaag
     1381 gcctcggcca gtccctcccc ggtcagcgtg tcgacccccg agtcgggcga cgccgcgagt
     1441 gggtcgtggc cggcctcgcg cagcttctcg acgagcttgg atccgatgag gccggttccg
     1501 ccgatcacga cgatcttcat cgcgctgcct ccctcgcgat cacgtcgtcg cctcagccgc
     1561 cgacaggatg acccggacga cgacgtctgg acgtgtgatc aggctcaggt ggccggtctt
     1621 gacggtggag atctgggcgc cggcgcgctc gtacatctct tcctggaggg ttggcggaat
     1681 cacgttgtcg agtgtgccga ggagcgccca cgacgggatc gtcttccacg cgggtgcccc
     1741 ggagggatcc tcgaactgcg cggccgacgc gggtcgctgc cccgccgcga ggacggctgc
     1801 ggtcctaacg ttgactccgt tggcgaaaca ctccatgaac ccggcgtacg gtggaaccgc
     1861 ttcccagcgc aggtacagat ccagcccgcc gtcgatcggc accgggttga acgcgccgtc
     1921 gtcggtgacg caggaggtgg tgcctcccac gaggtcgagg gccgtctcgt tctcgtcggg
     1981 gacgaacgcg tcgatgtaca cgagcgcctt cacgttgggg ttgccggtgg ccgcgttggt
     2041 gatcacgaag ccgccgtagg agtgcgccac gagcacgatc ggaccgggga ccgtctggag
     2101 gtagctggcc aggtacgggg cgtcctggct cggtccgcgc agcgtgttgg gcggagccac
     2161 aacggtgaat ccgcgatcct gcaggcgctc gatcacgccg ttccacgatg acgcatccgc
     2221 ccagtcgccg tggacgagca cgatggtcgg tcgcggatcc gagctcgcct gccgtgtggc
     2281 cgccgcatgc ccgccgccgc cgatccagac cagcgcgacg agaacgaggg ccgagctcat
     2341 cgcgatcatg cgccgagaaa gacgcgtcgg cttctcgctg atcattgaga cctcctcttc
     2401 ccgacggatg tcggtcacgg cgaggatgtc ccgaaagagg cgccgtcgaa tcagccgaca
     2461 cgccagcgtt ggtcgccagt cgctgacaga cgccggctcg gggcgtttcg ccaccacgga
     2521 gggccgggtg tacctttctc cggtcgcgag atggggggcg ggcgaggggc gttcgttcat
     2581 ctcgcgttca tccgtgagcc agcggtggga cgcctggtcg gtccacggtg gccgcgatcg
     2641 agcacacgcg ggtgctcgtg gaaggggacg atggcggggg acgatgtccg cgcagaacac
     2701 cctgcgaggg cgcgacgcag agtgcgcggt gctcgacgtc ctcctcgacg ccgcgcgcag
     2761 gggcgagagc cggaccgtcg tcctgcgtgg cgaggcgggc gtaggcaaga ccgcgctgct
     2821 ggactacgcg atcggctcag cccaggactt tcgcgtcctg cgatccgtcg gtgtcgagtc
     2881 ggagatggag ctcgcgttcg cttcgctcta tctgctctgt gctccgatcc tcgatcggcg
     2941 cggcgccctt ccgggtccgc agagagaggc cctggatgtg gtgttcggac ttgacagagg
     3001 cccggctccg gaccgattcc tcgttggact cgcgacactc agcctgctcg ctgagacagc
     3061 taacgagcgg cctctgctgt gtgtgatcga cgacgcccag tggctggaca gtgcatcagc
     3121 cctggccttc gcgtttgtcg cccgccgcct gttggcagac ccgatcggcc ttctcttcgc
     3181 ggtgcgcgag ccgatcgacg tgcgcgaatt ggcagggctt cccgagctag tggtcggcgg
     3241 catcagcgaa gacgacgccc gcatgctcct aggctcggtg ctgcccgaac cgctggacga
     3301 gcgggtgcga gaccgcatcg tcgccgagtc acacggcaac ccactcgcgc tgctcgagct
     3361 gccgcgggga atgcccccgg gcgagctggc gggcggattc gcccttcccg acgcgcggcc
     3421 cctggcggac cgtatcgagc ggagcttcct caggcaggtt cggtcgctcc cccccgattc
     3481 acaacggctg ctactcacgg cggcggcgga gccggtcggc gacgtcaccc tgctctggcg
     3541 cgcggccgag ccactcgggg tcgggcccga tgcggtcgcg ccggctgagg ccgccgggct
     3601 gatcgagctc ggcggccgcg tgcggttccg gcatccgctg gtgcgctccg cgatctatcg
     3661 cgcggcggca ccgaacgagc ggcgggagat ccatcgcgcg ctggccacgg cgaccgatcc
     3721 ggtggcggat cccgatcggc gggcatggca tcgtgcccat tccacggtcg gcctcgacga
     3781 ggcggtggcc ggcgagttgg aacgctcggc cgaccgggcc cggcgccgtg gcggctttgc
     3841 ggccatggca gcgttccttg aacgtgcgac ggagctgact cccgatcctg cacgccgggg
     3901 aacgcgagca ctcgccgccg cccaggccaa gctggaggca ggcgcccccg aagcggcgca
     3961 cgcgctggtg gcgaccgcgg aactcacccc gctcgacgag ctccagagcg cgcggttgca
     4021 gcggctgcgg gcgcagatcg cgttcgcccg caggcgcggc agcgacgcac caccactgct
     4081 gctcgacgcc gccaagcggc tggtcccgct cgaccccgag gttgcgcgcg agacgtgttt
     4141 ggaagcgctt gccgcggcga tcttcgctgg ccgcggcgtc aacggaaggg acgtgctgca
     4201 ggtcgtccac gccgcacccc cggcatcgcg gcccccaggc gcgacagacc tgctcctgaa
     4261 cggtctagcg acgcgagcga cggagggtta tgcggcgggc gtggctccgc tgcgagaggc
     4321 gctcgaagcc ttccgacgag acgacgggga cactccggcc aacaaccgct ggctctggct
     4381 cgcatgccgg gtcgccgcgg atctctggga gcacgagatc tgggaagagc tggccaccca
     4441 cggcgtgcgc cgcgcgcgca agaccggcgc tctcagcgtc cttcctatcg ccgccagcta
     4501 ccgcgccggc gcgcacgtgc acgccgggga gtacgcggag gcgtcggcgc tgctggagga
     4561 ggcctacgcg atcagccaaa cgacgcacac ggcgctcttg gttcaggcca ggcagatcgt
     4621 cgccgcgtgg cgcggcaacg aggccgaagc gctcgagctg atcggcgcgg gccgcccgga
     4681 ggcggccgcc agaggccagg gcatggcgct gagcatgatc gagtgcgcag acgcgctgct
     4741 ctgcaacggc ctcggccgct acgagggggc gctgcttgcg gcagagcgag catgcgcgca
     4801 agacgaactc tccctgtacg ccctggcgct ggtcgaactc gtcgaagcag cggtgcacag
     4861 caacaaacca aaggtcgccg cggcggccct cgagcgcctc agcgagcgaa cacgagcaag
     4921 cggaaccgag tgggccctag gaatcgaggc ccgctcgcgc gcgttgctga cggagaggac
     4981 ctcggccgaa tcgctctacg aggaggctat cgagcgactg gctcatggtc gcctcgcccc
     5041 ttacctggcc cgagcccagc tcgtatacgg cgaatggctc cgtcgcgaga accggcgcgt
     5101 ggacgcccgc gaacagcttc gcgccgctca cgacacgttc agccgcatcg gggccgaagg
     5161 gttcgccgaa cgctcccgtc gcgagctctc ggccaccggc gagacggtgc gcaagcgcac
     5221 cctggacacg cgcgaactcc tcacagccca ggaagcgcag atcgcccatc tcgcccgcga
     5281 cggcctctcc aaccccgaga tcggcgcaca actgttcatc agtccacgga cggtgcagta
     5341 ccacctgggc aaggtcttcg ccaagctcga cgtcacctcg cgcaaccagc tcagccgcgt
     5401 tcccgccagc cgcctcaccg tgtcgtagca gtcccgacct ccgcgggccg ggcacggcgc
     5461 tcacgatccg cgcgccgatt cgggatcgaa gtgaggtcgc cacgttctgc gcacagccat
     5521 cccgcctgac gaacgagcgt cacgctagcg gcgatctatg ttgctggcgc tggtcggagt
     5581 gcgatggtgg gacgcgactg gcgacctcac tggcgcttcg gctgattcga gcaccccccg
     5641 gtcgcggcat gctcgctcca cggtctcatg acgcgctcga gccgaggact cgagcccggt
     5701 gtacgagcgg agaggtgatg gagatgatcg acgcggtcgc ggtcgatgaa gcagcgctca
     5761 cctgcccgtg tcctcccggt ctcgaacgtc ctaggtgaag aggtcgacga gggaaagggg
     5821 agccacgatg gcagaggtcc cggccaaggc caagcctgac acgattgtgc tgatccacgg
     5881 cttctgggtg acgccgagga gttgggagca ctggaaggag tactacgagg cgaagggcta
     5941 tggggtcctc acacctgcgt acccggggtt cgaggtcgag gtcgaggcgc tccgcgccga
     6001 cccaacgccg atcgaacagc tccaggtgcc ggagatcatc gacatgctcg agaacatgat
     6061 cgggaagttg gatcgtccgc cgatcatcat cgggcattcc gccggcggcg tgttcaccca
     6121 gatcttgctc gatcacgggt tcggcgcggt cggggtggcg atgaactcag cgccgaccga
     6181 gggcgtgaag gtcgttcccc tctccgagat caggtccggg ttccccatcc tgaagaaccc
     6241 cgcgaaccgg catcgagcgg tcggcttcac gccgaaacag tggcactacg tgttcgcgaa
     6301 cacgttcagc gacgagaagt cactcgagct ctacgagcgc taccacatcc cagcctcagg
     6361 cagagtcttc tggggaagcg cgctcgcgaa catccacccg ggaaaggacg acacctacgt
     6421 cgactaccac aacgacgcgc gaccgccgct gctgttcatc tctgggaagc aggatcatct
     6481 cttccctccg aaggtgggcc actcgaatgc gaagcactac aagtccaaga cggtgacgga
     6541 gatcgtggag ttcgatggcc cgcatctgat gcccgccatc gatgggtggg aggaggtcgc
     6601 cgactacgcg ctcgactggg cgctcaagca cgcggagtga cggtgcttgc gagtaggacc
     6661 tccgccgcag cgggcctccg ttctggctga agacctcgct gcattgaaaa gggagacgac
     6721 gatgaacgca gctggagtag ccgaggtgat ccgcgaggcg ctcgacagca ggggccaatc
     6781 gagcaacata aaggcgacgg gggatcgagc tgctcagttc gagctggcga cgatggcgga
     6841 cgggatggcc cgagtgtttg agatcacggt cgaggagcgt ggctagcggc cgctcagctc
     6901 ccgcgggcgg aagcaaagat cttacgtgcg cattgaccag gacgcacaat gaggagagcg
     6961 atgaacatca ggttcagcaa ggtgtggatg cggcggtggc ggtgcgtcgc cggccactgg
     7021 tacgacgtcg aaacgtcggc acccgatcgc gacgggtgag caggatgaag cgcatccgga
     7081 tggagcgacg aatgttcaga gggagcacct gtacgactca ctcccgctcg atccacgcga
     7141 cgccgatatc ctgcgggcca agcagctcat gtatgtccga cgctcccacg acgaaggagg
     7201 gagccgtgct tgaggtcgga cgagatgttg agatccacca ggaagagagc gaaccacccc
     7261 gcctcgctcg ttccgatgtc tgaatactcg atcgacgacg tcgccgcacg ggcaggcgcc
     7321 gctcacgact acgtcagcag gctggtcgag ctcggggttg tctctgttgg tgaaggaggc
     7381 ggcttctctg aaggcgatgt caggcgggtc cgcctgatgc agaccctcga gcgggcgggt
     7441 gtcccgctcg aggaagtggc cgccgcgatc cggcgaggcg agctctctct gggcttcttg
     7501 gacatgtcgc acttcacgcg gctgtcgtcg ctcagcggtg tcacgtttca agagctctcg
     7561 gagaagacga acgttcccgt cgagctgttg ctggtcatcc gcgaggcagc ggggttcgcc
     7621 gaggctcatc caacggatcg cgcccgcgag aacgagctcc agatcgtccc gctcatcgaa
     7681 gcccaggtcg cgttgggctt ccgcctcccc gtgatcgagc gctggctgag ggcgtacgga
     7741 gacagcctgc ggcgcatcgc ggagaccgag ggagagtggt ggcacacgga gatcgagctt
     7801 cctctcctcg aatccgggat gacggcggcg gagatgatgg aggccgcgga cggccgggtc
     7861 gttcaccgga tcgtgccctt cctcgaccag gccctcgtcg ccttgtacca cggccatcag
     7921 gaacacgcgt ggacgaagaa cttcctcgac ggaatcgagg cggcgctctc aggagctggc
     7981 gtccacagcc gggtggagcg ccccccggcc atctgtttcc tcgatatcgc aggctatacg
     8041 aggctaaccg aggagcgcgg cgacgaagcc gctgccgagc tcgcgggaac gatgtcccgg
     8101 atcgtccaac gcacttcgac gtctcatgga ggcaggccca tcaagtggtt gggtgatggg
     8161 gtgatgttcc acttccctga gccggggccg ggcgtgatcg cggcgctcga catggtcgag
     8221 ggcatcgcgg gcgccggtct tccgccggcc cacgtcggac tgcacgcggg ccccgtcctg
     8281 ttccaggaag gcgactactt cgggcgcacg gtgaacatcg cgtcgcggat cgccgactat
     8341 gccagacctg gtgaggtcgt ggtgagccaa ccggtcgtcg acgccgccga agcgacaccc
     8401 gtctcgttcg tcgagatcgg ccccgtcgag ttgaagggac tctcagagtc cattcgactc
     8461 cacaccgccc gccgcgatgc tcgggcaccg aacggctgag tctctgatgg cgtccagcac
     8521 ctcacccaca tccagcgatc gaaccgaagg aggggagcca tgatcgaggt ccggcgtgac
     8581 gacgagatcc acgaggaggc gggtggctgg ttcgatgccc gctggcactt ctcgttcgac
     8641 cactaccgcg attctcgtca gatgggcatc ggcccactgc gagtgttcaa cgatgaccgt
     8701 ctggcgccgg ccgccgactg gccgatgcac cctcacgccg acgtcgaggg gctcacgtac
     8761 gtggtcgagg gcacgttcga gcacgccgac tccctcggca acggcgggac gctgctcccc
     8821 gggggcgtcc agcggatgac gctcggctcc ggcgcctacc actccgagcg gaacggatcc
     8881 aagaccgagc cgatgcgctt tctgcagttc tggatcctcc cggacacccc ttgctgccgc
     8941 cgagcctgga acagcggcag ttcacgctcg atgaccgtcg gaaccgcctg ctgcacgtga
     9001 tcgatccaga tggcggcgac gtcgtaaggg tgcactagga cgcgtcggcc ttcgtcggct
     9061 cgctcgaccc cggcgtcgga gcagggcaca gcctcaggga gggccgggcc ggaacctcta
     9121 cgtgatcggc ggcgcgaccg agctcaacgg aaccgacagg ctcgacactg gtgacgccgt
     9181 aaaggcattc gggcccgagg agctccgcat tcaagcactg gagcagacgg agcttatcct
     9241 catcgacgtg cctcaccagt acgtaccggt cggagtgtgg gctccgtgat gctgcgattg
     9301 cgcgctcaag gcgatccggc cacctccagc cagctcgcct tgccgagccc gaggacgtag
     9361 ttgtctcgcg ctcgcgtgag gacgagcagg acgccctcgc agtggcagca tcgaaggacg
     9421 acgctggggg cgtccatgta ggcgtgctcg gcaccgatcg cctcgatcct cccgcacccg
     9481 ccgcaggcga cccgggccgc cgtcatctcg tgcacgaaga tctcgccgag catccccgcc
     9541 atcgcgttgc cgtcgaccct gctcacctgg ctctccatct atcctcccgt cggtccgaat
     9601 ctctcggtct tgacgctggt cggccgatag ccgatgagta ccaaggcact ggcgaccgac
     9661 tccacgaagg gcgtcggccc gcacacgaac gccatgggat cggcggacgg agcccaggcg
     9721 gcctcagcga gcatcgcgcc gtcgactcga cgagcgtagc cggtccatcc gggcggctgc
     9781 gttcgcgtca gcgtgtgaat cacctggggg cctccggcgg ccgaggagag ccgttcgagc
     9841 tcctcgcggt agatgacgtc ctcccacgac cgcgaggagt acacgaggcg agcgcccggg
     9901 acggtggccg aagcctctcg gtgccggagc atcgacatca acggcacgat cccggatcct
     9961 ccggcgacga gaagcagcgg ttcctgcctg ctcgcctccc acacgaagta cccgccgatc
    10021 ggcccgcgga gctcgagcat gtcccccggg cgcagctcac cgaccaggta cgaggaaacc
    10081 tctccgtcgg gcaggcgctc cacggtgagc gcgagccggg gatcttccgg cgcagaggcg
    10141 atcgagtagc tccgctgggc ctggtagccg tcctcggccg tgaggcggac gtccacgtgc
    10201 tggcccgctc ggtgcctcgg ccagtccggg acgtccagga cgatgctccg gacccgcggc
    10261 gtctcgtcga tcagctcgac gacctcgccg agcgtccagc tcagtcgccc cagtaccgct
    10321 gctctcgcca tggatcaccg tagatgtggt agccgtagga ctcccagaac cccggctcgt
    10381 cgtgatccat gagccgaagg ccgcgcaccc acttcgcgct cttccagaag tacaggtgag
    10441 gaacgaggag ccgggcgggt ccgccgtgtt cggcctccag cggctggccg tcgtaggcgg
    10501 agacgaccca cgccttgccg tcgacgaggt cctcgagcgg caggttcgcc gtgtagtcgc
    10561 cgtcggagaa ggccatggcg tgtgtcgcgt catgttcgac gccttccatg agcgtgtcga
    10621 tcgagacgcc ctcccacgtc gtgtccagct tcgaccactt cgtgacgcag tggatgtccg
    10681 tgcggatctc ctcgctcgga agggcgcgga actcctccca gctccagctc ttgagcacgc
    10741 cgaggtcacc ttcgatggag aacgtccatt cctcgagcgg ggtgtgcggc gtgggaccgg
    10801 ccgacagtac cgggaagtcg tccacgaggt actggcccgg cgggagtcga gaatcctccc
    10861 gttcttcagc gcgcttgccc cgaaacccgc gcgagatgta ggtcatgacc cacttcccca
    10921 ccagcgccgc gtgaatggca cctcgtagga tgaacctagc ggctcggggc acgcaacccg
    10981 gagctgcttc ctcgggttag tcatgactgc cacctagaag tcaccgccag attgttcaac
    11041 tgtctgatcg accctgatgg aacgcacccg ctcgtcgtct ggggacgcct gcacctgccg
    11101 acacggccgt gacatgatgc ggtgtatggg agaagacttc gtgcgagtgt tctcaacgca
    11161 gtcgctggtc gagggagaaa tcgtcaaggg ccgactcgag gctgaaggca tccccgtcga
    11221 tctcagggga gagcgcgaag gcccataccc gctgggtccg gccgagctct tcgttccatc
    11281 gaggttcgag gcacaggcgc gccgagtgat cgagcagatc gagcagatcg agagcggctc
    11341 gtatgaggcc cctgacggga atggtgcgga gtagatcgcg acgccgcccc atcggcaccg
    11401 gcggtatctt tcgcatctcc gggataggcg gtcacggtag gccgcgactt gagctccttc
    11461 acgacgcaaa cggcgaccgc gacgaccggt acggcgagca ccatcccggc gacgacaaag
    11521 aggcccctcc gccgatgacg ctcacaagga tcacgacggg gtggaggacc agcgagcgcc
    11581 ggacacc
//
LOCUS       DASVEU010000247         8263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1538157,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000247 DASVEU010000000
VERSION     DASVEU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538157"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF225_10505"
     CDS             complement(<1..485)
                     /locus_tag="VF225_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145725.1"
                     /translation="MARYLFALSLLIGTIVPAATALAARSQQPMADSGSIGIRIVDVP
                     VAARNDPLALTYIVNRITPGKTVRRRVEIRNSTQANASVLVYPAAASLRQGGFAFAAG
                     HGRNELSGWTSLSRTALRLPPGANAFATVTIRVPKDASSGERYAVIWAEVSTSGSGGV
                     KL"
     gene            complement(502..1011)
                     /locus_tag="VF225_10510"
     CDS             complement(502..1011)
                     /locus_tag="VF225_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145726.1"
                     /translation="MRKSLLLACALTTVAITTVMPAGAATPATFTLLAGTLSISTPTV
                     SVSLGSQVSLITSSTISGPLGVVTVSDQRGGATTWTASVISTAFTPPAGPADPASNVS
                     YAAGAITASATVVATAVAAPNLTGVSTVVTGTSTGVSSASWNPTISVVVPANFAPGEY
                     AATITHSVA"
     gene            1281..1487
                     /locus_tag="VF225_10515"
     CDS             1281..1487
                     /locus_tag="VF225_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145727.1"
                     /translation="MAKLTRERKVKEKRELKEEKKAAAAAAKAAGTYSYDGEPVEAPA
                     LDAIDSTDEAATPDPAVAAAGEDR"
     gene            complement(1484..2851)
                     /locus_tag="VF225_10520"
     CDS             complement(1484..2851)
                     /locus_tag="VF225_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145728.1"
                     /translation="MNRLPIRLRLTLVFALAMAVVLVLAGWLVYARVSNDLANTLDEQ
                     LRSRAQDLSALVRREGSLKSTHGSLIEHGETFAELLSATGSAVDSTNPVGHTPLLSPR
                     DLSHALRGRFFLNRNSVPGLDEPARMLALPVPRGRTRLVLIVGATRANRAETLVSLRN
                     GFLIGGPLALILASLAAYGLAGAALRPIEAMRRRAADISASSLEDRLPVPPSGDEVSR
                     LGETLNEMLARIADGLARERRFVADASHELRTPLALIKTELELALRRSRTTAELETAI
                     RGAAEDTERLSRIADDLLLLARAEQGRVSLRREPVDVADVLETVAGRFKSRSESEGRD
                     LSVTSDDDPLVVSADRSLIEQALANMVDNALTHGAGRVTLRAEQRNGSAELHVVDEGE
                     GFPADFLHLAFERFSRAQKTTADGSGLGLAIVETIAEAHDGRAGAANASGGGTDVWLS
                     LTVAR"
     gene            complement(2848..3528)
                     /locus_tag="VF225_10525"
     CDS             complement(2848..3528)
                     /locus_tag="VF225_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145729.1"
                     /translation="MRVLIVEDELKMASLLRRGLLEEGHAADVATTGEDALWMAQSHP
                     YEAIVLDVMLPGLSGFETCRQLRNAGIWAPVLMLTARGGVDDRVAGLDVGADDYLTKP
                     FSFAELLARLRALIRRGGGERPTELVVGTLRLDPASRRAWRGRTEISLSPKEFALLEA
                     FMRRPGQVLSRLQLLEHAWDFAYENRSNVIDVYVRYIREKVDRPFGTESVETVRGIGY
                     RLRESDEE"
     gene            complement(3735..4319)
                     /locus_tag="VF225_10530"
     CDS             complement(3735..4319)
                     /locus_tag="VF225_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145730.1"
                     /translation="MLEITPGLRRWTAFHDHWEEDVGSLAVQTSDGLVLIDPIDPPNE
                     VAAADHVLITVYWHGRSTGTLGAKRVWASARSERPLKSRGIAVTDPFRAGNALPGGIR
                     AFQTARSAEVVYWLPDQRAVVVGDVLLGAGAKPRPTSDPLRLCPQRWLGKETHDDLRS
                     TLRPLLDLPVERVLVSHGEPILSGGKQALAAALD"
     gene            4700..4969
                     /locus_tag="VF225_10535"
     CDS             4700..4969
                     /locus_tag="VF225_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145731.1"
                     /translation="MSVQGNAIVLVRMEPASGFDLNTGEGVMTYKGPKRIDGAAAGAS
                     VVQEVVRTGDFEAVLSWAIGLSDKVDFRVQTATSPTRLIVDFRNH"
     gene            4973..5359
                     /locus_tag="VF225_10540"
     CDS             4973..5359
                     /locus_tag="VF225_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7145732.1"
                     /translation="MTGHAEREQVLAQLREICLALPETSERPSHGAPTFFIREKRSFL
                     MVLTNHHGDGRFAIWCAAPEGMQRMLVDADAERFFVPPYVGHRGWLGLRLDREIHWDE
                     LTGIVEDAYAEVAPAKLVEAARLGHT"
     gene            5428..7020
                     /locus_tag="VF225_10545"
     CDS             5428..7020
                     /locus_tag="VF225_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7145733.1"
                     /translation="MADYSAHEKDRRMLHALGYAQELRRGMSGFSNFAISFTIISILS
                     GTLTLYATGINYGGPVEEAYGWPIVSFFVIIVALGMAEIASKYPTAGGLYYWASKMGG
                     PVWGWFTGWFNLIGQIAITAGIDYGAAIFTDALLNLLWPGTFHASPHEIIYVYACILG
                     LHALMNIFSVRLVALLNDISVWWHVFGVLIVVGFLAFKPDHHQSVGTVFSKTINNSGF
                     SHQWLWFVLLLGLLQAQYTFTGYDASAHMSEETQNAGRAAARGVVMSVVVSAIFGYIL
                     ALGVTFAIQSFESTTGAGIFAVKQVLLDSLGLRAAEFLLFIIVGAQLYCGMSSITSAS
                     RMLYAFSRDGATPGHRIWRRLNTARVPYMAVLAIAVLAFLCAFPAYFSKDIGVGAGYV
                     AYAAVTSIATIGLYIAYALPIFLRLRLGDAWEPGEWNLGRWYKPIGWTAVIWVGFISV
                     LFIMPITPTGIPFKSGFTWLSVNYAPIAVVGAMLLVGGWWLLSAHKWFKGPVAQGSEE
                     ELARIEERYGERTGPPVTTPAA"
     gene            7076..>8263
                     /locus_tag="VF225_10550"
     CDS             7076..>8263
                     /locus_tag="VF225_10550"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7145734.1"
                     /translation="MLTLNELKRDVENGSIDTVVVAFTDMQGRLLGKRVHAQYFLEEH
                     IAEHGVEGCNYLLALDMEMEPVPGYSIASWEQGYGDFAMRPDLDTLRRIPWLEGTALV
                     LCDVTWNDGLPVRPSPRQVLKAQVERAQAAGYEPMIGSELEFYLLKESFEEAHAKHYR
                     DLTPSVPYILDYHVLATTYDEPLLRQIRNGMYEAGITVESSKGEAWPGQQEVNFRFAD
                     AVTMADNHSIYKNGAKEIAHLNGCSITFMAKPDHSWIGSSCHIHASLWRDGENAFAGG
                     PEVFEQFLAGWIAGAKEMAIFLAPTINSYKRYAAGSWAPTTLAWGHDNRTCGFRIVGH
                     GAAQRIETRIPGGDVNPYLAFAALIAAGLHGIEAGLELPPALEGNAYESDAERFPSTL
                     REAI"
BASE COUNT         1387 a         2650 c         2815 g         1411 t
ORIGIN      
        1 agcttgacgc cgcctgatcc cgatgtggat acctcggccc agatgactgc gtagcgctcg
       61 ccggacgagg catctttcgg cacgcggatc gtgactgttg cgaaggcgtt ggcgccaggc
      121 ggcaggcgca gtgcggttcg gctcagcgac gtccagccag agagctcatt tctgccgtgg
      181 ccggcggcga acgcgaatcc gccctgtcga aggctggcgg cggctggata gacgagaaca
      241 gacgcgttcg cctgggtgct gttcctgatt tcgactcgac ggcgaacagt cttgccgggg
      301 gtgattcgat tgacgatgta agtgagggcg agggggtcgt tgcgtgcggc gaccggcacg
      361 tcgacgatcc gaatcccgat gctgcccgag tctgccatcg gctgctgtga ccgtgccgcg
      421 agtgcggtgg cagccgggac aattgtcccg atcagaagcg aaagcgcgaa caggtagcga
      481 gccacggcgt gttctcctcg cttaggcgac cgagtgggtg atcgtggccg cgtattcgcc
      541 gggggcgaag ttcgccggca cgaccactga gatcgtaggg ttccaactcg ccgagctgac
      601 tccggtgctt gtgcctgtca cgacagtcga cacgccggtt agatttggtg cagcaaccgc
      661 cgtcgcgact acggtcgcgg aggccgtgat cgcgcctgct gcgtagctga cgttgctcgc
      721 ggggtcggcc gggccggccg gaggagtaaa ggcagtagag atcacagatg ccgtccaggt
      781 cgttgcgccg ccgcgctggt ccgagaccgt gacgacaccg agcgggccac tgatcgtgct
      841 cgatgtgatc aatgagacct gtgatccgag cgacacgctg actgtcggtg tgctgatcga
      901 caacgtgccc gcgagcagcg tgaaggtggc cggggtagct gcccccgcgg gcatcacggt
      961 cgtgatggcc actgttgtca aggcgcaggc aaggaggaga gatttccgca tgaatagatc
     1021 cttcgttagg tcgtgggagc aggccgggag ccggccgtac tcgttgtgat cggcatccca
     1081 ctccgacacc gggagggtgg gatgggtcac ccgtttcgga ggttcgggcg cgccgaatcg
     1141 ggggtgccgc cggccccagg agccacgtac gcaacgtacg tccgcctctg gctccgtcag
     1201 cgtccggcgg cttgttcaag ctattctgga cgcgtcaata gagaggaagg gctgcattgg
     1261 gctccaagag acagacgacg atggccaaac tgacacgcga gcgcaaggtg aaggaaaagc
     1321 gcgagttgaa ggaagagaag aaggcagcgg cagccgcggc caaggccgcc ggcacttact
     1381 cgtacgacgg cgagccggtc gaggctcccg cgctggacgc gatcgactcg actgacgaag
     1441 cggcgacgcc cgacccggca gttgccgcgg ccggcgaaga tcgctaacgc gcgacagtca
     1501 gagacagcca gacgtcggtc ccaccgccgc tcgcgttcgc agcgcctgct cgtccatcgt
     1561 gcgcttcggc gatcgtttcg acgatcgcca ggccaagacc gctgccgtcc gcggtcgtct
     1621 tctgcgcccg gctgaaacgc tcgaacgcga ggtggaggaa atccgcgggg aaaccctcgc
     1681 cctcgtccac cacgtgcagc tctgcagatc cgttgcgctg ttcggctctg agggtcacac
     1741 ggcccgcgcc gtgagtgagc gcgttgtcga ccatgttcgc gagtgcttgc tcgatgagcg
     1801 aacggtcagc cgagacgacg agcggatcgt catccgaggt caccgagagg tcacggcctt
     1861 ctgactccga gcgggatttg aaacgtcccg cgaccgtctc gaggacgtcg gcgacgtcga
     1921 ccggctcgcg ccgcaacgac acgcggccct gctcggcgcg tgccaggagc aggaggtcgt
     1981 ccgcgatccg ggagagacgc tcggtgtcct cggctgcgcc gcggatcgcc gtctcgagct
     2041 ctgctgtagt acgcgaccgc ctgagcgcca gttcgagctc ggtcttgatc agtgcaagcg
     2101 gcgtcctcag ctcgtggctc gcgtccgcga cgaaacgacg ctcacgcgcg agcccgtcgg
     2161 cgatgcgcgc cagcatctcg ttcagcgtct ctcccagtcg cgagacttcg tctccgcttg
     2221 gcggaaccgg cagccggtcc tcgagcgacg aggccgaaat gtcggccgct cgccgccgca
     2281 tcgcttcgat cggacgcaag gcagcaccgg cgagcccgta agcggcgagt gaggcgagga
     2341 tcagggccag cgggccgccg atcaggaaac cattgcgcag actgacaagc gtctccgctc
     2401 tgttcgcacg tgttgcgccg acaatcagca cgagccgggt ccgcccgcgt gggacgggca
     2461 acgcgagcat gcgcgccggt tcgtcgagcc ccggaacaga gttccggttt agaaagaaac
     2521 ggccgcgtag cgcgtgcgag agatcgcgag gcgagagaag cggcgtatgc cccacagggt
     2581 tcgtcgagtc gaccgccgag ccggtcgcgc tcagcagctc tgcgaaggtc tccccgtgct
     2641 cgatgaggga gccgtgcgtg gacttgagcg agccttcacg gcgcacgagc gctgagaggt
     2701 cctgtgcgcg cgagcgcagc tgttcgtcga gggtgttggc aaggtcgttc gacacgcgcg
     2761 catagacaag ccagcccgca agcaccagga cgacggccat tgcaagggcg aagacgagcg
     2821 tcagccggag cctgatcggg agccggttca ttcctcgtca ctctcgcgca gccggtagcc
     2881 gattccgcgc acggtctcga cggactcggt cccgaagggc cgatcgacct tttcccggat
     2941 gtaccgcacg tagacgtcga tgacgttcga tcgattctcg tacgcgaagt cccaggcgtg
     3001 ctcgagcaac tggagccggg aaagaacctg tccgggtcgg cgcatgaacg cctccagcag
     3061 cgcgaactcc ttgggcgaga ggctgatctc cgttcgaccg cgccacgctc gtcgcgaagc
     3121 gggatcgaga cggagcgtcc cgacgaccag ctccgtcggc cgctcgccgc cgccccgcct
     3181 gatgagggcg cgcagccgcg caaggagctc ggcaaaggag aagggcttcg tcaggtagtc
     3241 gtctgcgccg acgtctaggc cggcgacacg gtcgtcgaca ccgccgcgtg ccgtcagcat
     3301 gagaacaggc gcccagatgc ccgcgttccg gagctgccgg cacgtctcga aaccgctcag
     3361 ccccggaagc atgacgtcga ggacgatcgc ctcgtaggga tgggattgcg ccatccagag
     3421 ggcgtcctcg cccgtcgtgg ccacgtccgc cgcgtgtccc tcttccagca aacctctccg
     3481 caggaggctc gccatcttca gttcgtcctc gacgatcaag acgcgcacca ctcaagtgtg
     3541 ccgtcgttcc atgagagtcg gatgagaaag aagagaggca gatagcctcg gggccggcga
     3601 caccgagacg aacatcgtcg cgggcacgac catcaccggt gacttcatat gccgggcgac
     3661 gaacgtcgac taccggatgg acacgccctc cgctcggtca ttcctcgagc agttcgtcac
     3721 gcttccgtag ttgatcagtc cagcgcggcg gcaagcgcct gcttgccgcc gctgaggatc
     3781 ggttcgccgt gcgagacgag cacacgctcg accgggaggt cgaggagggg acgcagcgtg
     3841 ctgcgcaggt cgtcgtgcgt ttcctttccc agccagcgtt gcgggcacag acgtagcggg
     3901 tcggaagtcg gacgcggctt cgcgccggcg cccaggagga cgtccccgac aacgaccgcg
     3961 cgctggtccg gcagccagta cacgacctcc gccgatcgcg ctgtctggaa ggcgcgaatg
     4021 ccgccgggaa gcgcgttccc ggcgcggaac ggatccgtca ctgcgatccc ccggctcttg
     4081 agcggccgtt cggaacgggc cgaggcccac actcgcttcg cgccgagcgt ccccgtgctg
     4141 cgtccgtgcc agtaaacggt gatgagcacg tggtcggctg ccgcaacctc gttcggcgga
     4201 tcgatcgggt cgatcagcac tagcccgtcg ctcgtctgca cggcgagcga gccgacgtcc
     4261 tcctcccaat ggtcatggaa ggcggtccag cggcgcaacc caggtgtgat ctccagcatt
     4321 gacgccatct tccctaacaa ggcctcagta ggctgcaccc atccgcaggc tcttctccat
     4381 cggtgtcgtc accgcggccc tgctggcggt tgccggctgc ggcgggtccg acaagtccac
     4441 gactccgcag ggcggctatt cgcttccttc gccgacgccg acgacgacta gcaccggtgg
     4501 gaccgacccg ctcgagggcg cgggcacgac ccctgtcgtc ggtagcgcga gcggcagcga
     4561 gaccgcgctc ctcgaccgca tcgccgtcgg ccggcacgag ggctacgacc gtgtcgtctt
     4621 ccagttccag aatgtcctac ctggctatcg catcgagtac gtccagccgc cgctgaagga
     4681 ggacgggtca ggcaacccgg tgagcgtcca gggcaatgcg atcgtgctcg tgcgcatgga
     4741 gccggcatcg ggcttcgacc tgaacacggg cgagggcgtg atgacctaca aggggccgaa
     4801 gcggatcgac ggcgcggcgg cgggcgcctc ggtcgtccag gaggtcgtcc ggaccggcga
     4861 tttcgaagcg gtgctgagct gggcgatcgg gctctcggac aaggtcgact ttcgtgtcca
     4921 gacggcgacg tcgcccaccc ggctgatcgt cgacttccgt aaccattagg gcatgaccgg
     4981 acacgccgag cgcgaacagg tcctggcgca gctccgggaa atctgtctcg ccctgcccga
     5041 gacgagcgag cggccgagcc acggcgcgcc cacgttcttc atccgcgaga aacgctcgtt
     5101 cctgatggtg ttgacaaatc accacggcga cggacgcttc gcgatctggt gcgcggcgcc
     5161 ggaaggcatg cagaggatgc tcgtggacgc ggatgcggag cgcttcttcg tgccgcccta
     5221 cgtcggtcat cgcggctggc tcgggctgcg cctggaccgc gagatccact gggacgagct
     5281 caccgggatc gtcgaggacg cctacgcgga ggtcgctccc gcgaagctgg tcgaggccgc
     5341 ccggctcggc catacgtagc ccttctattt gatccggggc ggccggcgcg agagaatccg
     5401 cgccgacccc aactaggagg cggaactgtg gccgattatt cggcgcacga gaaggaccgg
     5461 cgcatgctcc acgccctcgg ctacgcccag gagctgcggc gcgggatgag cggcttctcc
     5521 aacttcgcca tctcgttcac gatcatctcg atcctgtccg ggacgctcac gctctacgcg
     5581 acgggcatca actacggcgg gccggtcgag gaggcctacg gctggccgat cgtctcgttc
     5641 ttcgtgatca tcgtcgctct cgggatggcc gagatcgcat cgaagtatcc gaccgcgggc
     5701 ggtctctact actgggcctc gaagatggga gggcccgtct ggggctggtt cacggggtgg
     5761 ttcaacctga tcggacagat cgcgatcacc gccggcatcg actacggcgc ggcgatcttc
     5821 accgacgcgc ttctgaatct gctgtggcca gggacgttcc acgcctcgcc acacgagatc
     5881 atctacgtct acgcctgcat cctcgggctg cacgccttga tgaacatctt cagcgtcagg
     5941 ctggtcgcct tgctgaacga catctccgtc tggtggcacg ttttcggcgt gctgatcgtc
     6001 gtcggcttcc tggcgttcaa gccggatcac caccagtcgg tcgggacggt cttctcgaag
     6061 acgatcaaca acagcggctt ctcgcatcag tggctctggt tcgtgctgtt gctcgggctc
     6121 ttgcaggcgc agtacacctt cacgggctac gacgcttcgg ctcacatgag cgaggagacc
     6181 caaaatgcgg ggcgagcggc agcgcgagga gtggtcatgt cggtcgtcgt ctcggcgatc
     6241 ttcggttaca tcctggctct gggcgtcacg ttcgcgatcc aaagcttcga gtccacgacg
     6301 ggagccggca tcttcgcggt gaagcaggtc ttgctcgact cgctcgggtt gagggcggcg
     6361 gagttcttgc tgttcatcat cgtgggcgct cagctctact gcgggatgtc ctcgatcacg
     6421 tcggcttcgc ggatgctgta tgccttctcg cgggacgggg cgacgcccgg ccatcgcatc
     6481 tggcggcggc tcaacacggc gcgcgtgccg tacatggcag tgctggcgat cgcggtgctg
     6541 gcgttcctct gcgcgtttcc ggcgtacttc tccaaggaca tcggcgtcgg cgccggctac
     6601 gtcgcctatg cggcggttac gtcgatcgcg acgatcggcc tctacatcgc gtacgcgctg
     6661 ccgatcttcc tgcggctgcg gctgggagac gcatgggagc cgggcgagtg gaacctcggt
     6721 cgctggtaca agccgatcgg ctggacggct gtcatctggg tgggcttcat ctcggtgctg
     6781 ttcatcatgc cgatcactcc aacggggatc ccgttcaaga gtggcttcac gtggttgagc
     6841 gtcaactacg cgccgattgc cgtggtcggg gcgatgctgc tcgtcggcgg ttggtggttg
     6901 ctgtccgcgc acaagtggtt caagggccca gtcgcgcagg gatccgaaga ggagcttgca
     6961 cgaatcgagg agcggtacgg ggaacggaca ggtcctcctg tgaccacacc cgcggcgtag
     7021 gatcgattcg agtgggaggg agccggttcg agccggctcc ctccgtcctt cgccgatgct
     7081 gacgctcaac gagttgaaac gcgacgtcga gaacgggtcg atcgacacgg tcgtcgtcgc
     7141 gttcaccgac atgcagggcc gtttgctcgg caagcgcgtc cacgcccagt actttctcga
     7201 ggagcacatc gccgagcacg gtgtcgaggg ttgcaactac ctgctcgcgt tggacatgga
     7261 gatggagccc gtccccggct actcgatcgc gagctgggag caggggtacg gggacttcgc
     7321 catgcgcccc gacctcgaca cgttgcgtcg cattccctgg ctcgagggga cggccctcgt
     7381 gctctgcgac gtcacgtgga acgacggctt gcccgtcagg ccgtcgccgc ggcaggtgct
     7441 gaaggcgcag gtcgagcggg cgcaggctgc cggttacgag ccgatgatcg ggtcggagct
     7501 cgagttctac ttgctcaaag agagcttcga ggaggcgcac gcaaagcact accgcgacct
     7561 cactccgtcc gtgccgtaca tcctcgacta tcacgtgctc gcgacgacct acgacgagcc
     7621 gttgctccgt cagatccgga atggcatgta cgaggcgggc atcacggtcg agagctcgaa
     7681 gggcgaggcg tggccggggc agcaggaggt caacttccgc ttcgccgacg ccgtcaccat
     7741 ggcggacaac cattcgatct acaagaacgg tgccaaggag atcgcgcacc tgaacggctg
     7801 ctcgatcacg ttcatggcga agcccgacca ttcctggatc ggcagctcct gtcacatcca
     7861 cgcgagcctc tggcgtgacg gcgagaacgc cttcgcgggc ggccccgaag tcttcgagca
     7921 attcctcgcg ggctggatcg ctggggcgaa ggagatggcg atcttcctcg cgccgacgat
     7981 caactcgtac aagcgctacg cggcagggtc gtgggctccg acgacgctgg cgtgggggca
     8041 cgacaaccgc acatgcggct tccggatcgt cgggcacggg gcggcgcagc ggatcgagac
     8101 gcgcattccc ggcggcgacg tcaatcccta tctggccttc gcggcgttga tcgcggccgg
     8161 gctgcacggg atcgaggcag ggctcgagct gccgcctgcg ttggagggca acgcgtacga
     8221 gtctgacgcg gagaggtttc cgtcgacttt gagggaggcg atc
//
LOCUS       DASVEU010000248         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1149491,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000248 DASVEU010000000
VERSION     DASVEU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149491"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF225_10555"
     CDS             <1..266
                     /locus_tag="VF225_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145735.1"
                     /translation="MPSGYEFEGTLGEMTFREIVELHPLPGSEFRDALLRCAEECLDC
                     GANCTACADASLSERDVAELTGVTVLLHRARTKVGSALEEYMK"
     gene            263..511
                     /locus_tag="VF225_10560"
     CDS             263..511
                     /locus_tag="VF225_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7145736.1"
                     /translation="MRLDRELACRGIVELVTAYLDDALEPAGRERFEEHLAFCEGCNR
                     YLEQMQLTIEATGHLGVERLPAELEERLLKAFRGWRGS"
     gene            587..850
                     /locus_tag="VF225_10565"
     CDS             587..850
                     /locus_tag="VF225_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145737.1"
                     /translation="MDSLEPCRGGIRVALDCANFCDATARIATRQSRPDIRLVRAMIG
                     ACAAACVACAEECDRHAAHHEHCRICADVCRRCEAACDEVLAF"
     gene            1300..1821
                     /gene="msrA"
                     /locus_tag="VF225_10570"
     CDS             1300..1821
                     /gene="msrA"
                     /locus_tag="VF225_10570"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7145738.1"
                     /translation="MRGPVEKAILAGGCFWGMQDLFRKRPGVVSTRVGYTGGDVPNAT
                     YRNHGTHAEAIEIVFDPERTSYRDLLEFFFQIHDPTTLNRQGNDIGASYRSAIFYLDE
                     EQRRVAEETIADVEASGLWPGNVVTEVTPAGPFWEAEPEHQDYLERYPNGYTCHFVRQ
                     EWVLPRRTRAATG"
     gene            complement(1873..2745)
                     /locus_tag="VF225_10575"
     CDS             complement(1873..2745)
                     /locus_tag="VF225_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145739.1"
                     /translation="MAFSYRLVTLGRTGREHARPYTSQDPLAPGSTVLLGGRYWLVER
                     VDDWTVHARPARYRLTLRHPDGHEEAGAFRLFRADAPTMGHQLATNENREPVSWAVVE
                     QRLARDDTGEPFLEVIAERDHGEVESLPDHQLEHAVEQDRDGAVAAPAALAGATRASQ
                     AVELVALEPGQAPDWRQASRYLESLILEELEDDLIELCGVDTRNDPQEQWLDTVKRRL
                     REDLDSFRTDSEGLHDEIEEWDFRGSRIFAAVGRFDDDSNPASGYGWMCRLVDAGVLQ
                     AAGFYRARKPLLPL"
     gene            complement(2816..2938)
                     /locus_tag="VF225_10580"
     CDS             complement(2816..2938)
                     /locus_tag="VF225_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145740.1"
                     /translation="MGAAHGFSDGLAGLAEADDEQIGMQRFRVGFRAQPASSLR"
     gene            3181..3741
                     /locus_tag="VF225_10585"
     CDS             3181..3741
                     /locus_tag="VF225_10585"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX7145741.1"
                     /translation="MSHDEFHERGAHERQWHLAEVAGDALIAQEEARVGHADRGGQSL
                     NRLAVSATNHCLTGCLTGEVTGMVIATAFGWGDVASIAVAVGLGFLFGYLLTSLPLLR
                     AGLALGAIVPIALAADTVSIAIMEFVDNLTFVLVPGALEAGLGDLRFWLPLLGGFVIA
                     WPFALAVNHAMIGRGKGHAVVHEYHH"
     gene            4093..4488
                     /locus_tag="VF225_10590"
     CDS             4093..4488
                     /locus_tag="VF225_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145742.1"
                     /translation="MTFREIVELHPLPGSEFRDALLRCAEECLDCGASCTACADASLS
                     ESDLAELTGVIRVCLDCADVCEATARIATRQTRPDIRLVRATIEACAAACVACAEECD
                     RHAAHHEHCRICADVCRRCKAACDEVLAF"
BASE COUNT          746 a         1501 c         1642 g          754 t
ORIGIN      
        1 gtatgccttc gggttacgag ttcgagggca cgctcgggga gatgaccttt cgcgagatcg
       61 tggagctgca cccgctgccg ggaagcgagt ttcgagacgc tctccttcgg tgtgccgagg
      121 agtgtctcga ttgcggtgcc aactgcaccg cctgcgccga cgccagcctg agcgagcgcg
      181 acgtggcgga gttgaccggc gtgacggtgt tgctccaccg ggccaggacg aaagtgggta
      241 gcgcactgga ggagtacatg aaatgaggct ggaccgagaa ctcgcgtgca ggggaatcgt
      301 cgagctcgtg actgcgtacc tcgacgacgc gctcgagcct gccggtcgcg aacgcttcga
      361 agagcacctc gcgttctgcg agggctgcaa ccggtacctc gagcagatgc agctgacgat
      421 cgaggcgacg ggtcacctcg gcgtcgaaag actgcccgcc gagctggagg agcggctgct
      481 caaggcgttc cgggggtggc gcggatcgtg atcgcgtata agttcctcgc cgggcggcgt
      541 cgcgcccttc acgggcttcc gctggccagt cggcgagtgg gtcgaggtgg acagtctcga
      601 gccgtgtcgc ggtgggatcc gcgtcgctct cgactgcgcg aacttctgcg acgcgaccgc
      661 gcgcatcgca acccggcaga gccgtccaga catccgcctc gtccgcgcaa tgatcggggc
      721 ctgcgcggcg gcgtgcgtcg cctgcgccga ggagtgcgac cggcacgccg cccaccacga
      781 gcactgccgc atctgcgcag acgtctgccg ccggtgcgag gccgcatgcg acgaagtgct
      841 cgccttctga cgacggcgac gcatttgcgc gacatgcacg agagcggtcg cgttacgaat
      901 tacgtcgtcg acgttgagcg gcgccgacgg ccagcaggcc gcgggtgatg ttcggttttg
      961 tgcagccgca gtaccggccg tgcggcgagt gcggtgcatc agtgcctgtc agcgaggagg
     1021 acacgcacga gtgcgaccgg caacgctggc tggactacca gatgttccaa caccgagggc
     1081 gtgtagctca actcgaggta gaactcggtg tgtggctgga gacgccgcac ggccggttcg
     1141 ccgtgttcta cgcggaacga caacgacgaa gggaggccgg atggatctcg gcgagatcgt
     1201 gacggagatc gaggcgccgc cgccggtgac gaatgcccat tggactaacg agcccttctg
     1261 gcacgctcga agaggcgacg tttgggaagg aggcgggaca tgagaggacc agtcgagaag
     1321 gcgatcctcg ccggagggtg cttctggggg atgcaggacc tcttccggaa gcgaccgggc
     1381 gttgtctcca cgcgggtcgg atacacgggc ggggacgttc cgaacgcgac ctaccgcaac
     1441 cacggcacgc acgcggaggc gatcgagatc gtcttcgacc ccgagcggac ctcctaccgc
     1501 gacctgctcg agttcttctt ccagatccac gatccgacga cgctgaaccg tcagggcaac
     1561 gacatcggag cgagctaccg ctcggcgatc ttctacctcg atgaggagca gcgccgggtc
     1621 gcggaggaga cgatcgccga cgtcgaggcc tcgggccttt ggccgggaaa cgtcgtgacc
     1681 gaggtgacgc cagcggggcc gttctgggag gccgagcccg agcatcagga ctacctcgag
     1741 cggtatccga acggctatac ctgccacttc gtacgccagg aatgggttct gccgcggcgc
     1801 acgcgagcgg cgaccggcta gctcaaggcg gtgcttggtc gccgcggccg cagctcgcgg
     1861 cgccgctggg agtcagagcg gcagcagcgg cttgcgtgcc cgatagaagc cggccgcttg
     1921 gagcacgccg gcgtccacga gccggcacat ccagccgtag ccgctcgctg gattcgagtc
     1981 gtcgtcgaag cggccgacgg cggcgaagat cctgctgcct cggaaatccc actcctcgat
     2041 ctcgtcgtgg aggccttcgg agtcggtgcg gaagctgtcg aggtcctcgc gaagccggcg
     2101 cttgacggtg tccagccatt gctcctgggg atcgttgcgg gtgtcgacgc cgcatagttc
     2161 gatcagatcg tcctcgagct cttcgaggat caacgactcg aggtagcggc tcgcctgtcg
     2221 ccagtccggt gcctggcctg gttcgagcgc gaccagctcc accgcctggc ttgcccgcgt
     2281 ggctcctgcc aaggcggccg gagccgcgac cgcgccgtcc cggtcctgct cgaccgcgtg
     2341 ctcgagctgg tggtcgggca gcgactcgac ctcgccgtgg tcgcgctcgg cgataacctc
     2401 aagaaacggt tcgccggtgt cgtcgcgggc aagccgctgc tcgacgactg cccagctgac
     2461 cggttcgcgg ttctcgttcg ttgcgagctg atggcccatg gtgggggcgt ccgcccggaa
     2521 gaggcgaaac gccccagctt cctcgtgccc gtcgggatgg cgaagcgtca gccggtagcg
     2581 cgccgggcgc gcgtggacag tccaatcgtc cactcgctcc accagccagt accggccgcc
     2641 gagcagcacc gtcgaacccg gcgcgagcgg gtcttgactc gtgtacgggc gcgcgtgctc
     2701 gcggccggtc cggccaagtg tgacgagcct gtacgagaac gccatcagca gggcggatcg
     2761 taggctcagg cgagccggca atgggtgaca cggcaggaac tagcgcacgc agtggttacc
     2821 gaagcgacga ggcgggctgt gcacgaaagc cgacacggaa ccgctgcatg ccgatctgct
     2881 cgtcatcggc ttcggcaagg ccggcaagac cgtcgctgaa gccatgggcg gctcccacac
     2941 gaacctcggg aacggcagtg aatcctctgc acccgcgcat accttcggat tgcgagttcg
     3001 ccggcacgct cgggggatga ccttcagcga gatcgcgaag ctgcactcgc tgccgggaac
     3061 cgactgtcgt gaggcgctcc caaggtgtgc cgaggagtgt ctggattgcc gtgctagctg
     3121 caccgcctgc gccgacgcca gcctgagcga gagcgacctg ctgaagttga ccggcgttag
     3181 atgagccacg acgaatttca cgagcgtggg gcgcacgagc gtcagtggca cttggcggaa
     3241 gtcgccggcg acgccctcat cgctcaggag gaggctaggg tcggccacgc ggaccgcggc
     3301 ggccagtcgc tcaaccgcct ggctgtttcc gcgacgaacc actgcctcac cggctgcctc
     3361 accggcgagg tgacggggat ggtgatcgcg accgcgttcg gctggggaga cgtggcctcg
     3421 atcgccgtgg ccgtcgggct tggcttcctc ttcggctacc tcctcacctc gctgccgttg
     3481 ctccgggcgg ggctcgcgct cggcgcgatc gtgccgatcg cactcgccgc cgatacggtc
     3541 tcgatcgcga tcatggagtt cgtcgacaac ctgacgttcg tgctggtgcc gggggcactc
     3601 gaggcaggcc tcggcgacct gcgcttttgg ctgccgctgc tcggcggctt cgtgatcgcc
     3661 tggcctttcg cactcgccgt aaaccacgcg atgatcgggc gcgggaaggg tcacgccgtg
     3721 gtgcacgagt accaccacta agtttgggca cgggaaccga acagaagggc gatccttgcc
     3781 ggagggtgct tctgggggac gcaagatctc ttccggaagc aaccgggcgg tgtctccacg
     3841 cgggtcggat acacgggcgg ggacgttccg aacgcgacct accgcaacca cggcacgcac
     3901 gcggaggcga tcaagatcgt cttcgacccc gagcggacct gctagcgacg tagtcagcgg
     3961 ctgtcggact gtcttcgaga tgcgaccgag ctcgaagaac ccgcttccta tgcggatggc
     4021 gggaacggcg gcgatccctt tgcacccgcg catgccttcg ggttgcgagt tcgagggcac
     4081 gctgggggag agatgacctt tcgcgagatc gtggagctgc acccgctgcc gggaagcgag
     4141 tttcgagacg ctctccttcg gtgtgccgag gagtgtctcg attgcggtgc cagctgcacc
     4201 gcctgcgccg acgccagcct gagcgagagc gacctggcgg agttgaccgg cgtcatccgc
     4261 gtctgtctcg actgcgcgga cgtctgtgag gcgaccgcgc gcatcgcgac gcgacagacc
     4321 cgtcccgaca tccgcctcgt ccgcgcgacg atcgaggcct gtgcggcggc gtgtgtcgcc
     4381 tgtgccgagg agtgcgaccg gcacgccgcc caccacgagc actgccgcat ctgcgctgac
     4441 gtctgccgac gctgcaaggc cgcatgcgac gaagtgctcg ccttctgacg ggcgattctg
     4501 cgagctcgac caacgaccgt cggaatgggc cgcctcaggc gtcggtcttc tcgggccgga
     4561 cggcgtgggc gcgcagcctg cggacgccgc tgcgggaatt cctgcatacc gagacggccg
     4621 gcgcggcggt cttgctggcg gca
//
LOCUS       DASVEU010000249         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_956509,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000249 DASVEU010000000
VERSION     DASVEU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956509"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1261..2652
                     /locus_tag="VF225_10595"
     CDS             1261..2652
                     /locus_tag="VF225_10595"
                     /inference="COORDINATES: protein motif:HMM:NF041163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX7145743.1"
                     /translation="MSEDTQRSLSPAAARPLATTTKTVPQMEAITPRYLLRALPWVEV
                     EAGAYRVNRHRSFTVGDGLIGTEFDGDDARVFSEDLREMALLTDADSALLSALGESFG
                     QETFEPGAIITEPGTDADRLYVIAHGKVERWGATKYGEEALIDVLAEGDYFDGEAWVG
                     EQQWRHRVKAVTHCVLLALERARLTELVARDQQLAARFKELAASNGRRPGDSPIDLMS
                     GHAGEPELPQTFVDYEEVPREYEMSIVQTVLRVHTRVSDLYNEPMSQVTQQTRLTMEA
                     LREQQERQMLHHPDYGLLNNVSVKQRVRTRSGPPTPDDLDELLTRVWKRPAFFLAHPR
                     AIAAFGRECTRRGVPPAIMDVYGSPKLTWRGVPLLPSDKLDVNGTTDGSVGTTSIVLM
                     RVGEDERGVVGLRPAAVPDEVEPGMAVRAMGVDRRALTSYLLTAYFSVAVLVNDAIAV
                     LENVDVAKYHDYD"
BASE COUNT          420 a          920 c          946 g          436 t
ORIGIN      
        1 acgcggctcg ctggcgctcg gccctgccgg actgcccttg accgcccggc ttcgcttcgc
       61 tgatggtggc gatcatgggt tgcgctgcgc gcaatcagct cccgcgtgag cggcgctacc
      121 cacacgattc agcgaggagg ctcctttcag aggggctacg ccaggagatg cttcgcgcga
      181 tgctcgccta tgactcggtt tgctttcact gtcgtcgctg ggcggacgct tcacgcgttc
      241 acggcgcgct gtgagcgctt cctgcccggg cgcacgcggc gacggcgatc gcgtccgatg
      301 gcagggcaat ctgaccgacg tcggtcatcc cgccgcgacc gaagcctgcg ccgtgcgcgc
      361 gtcatccgcg tccagcgacg cggtgatctg gcgtgaacgc tcccgatctc tgaaaggcga
      421 ccgcagcctg ttcgtgcgcg tcgagcgcgc tgacccgtcg aagaacgcga tccgcggccg
      481 cgggcatacg agctgccgct ggagcgtcgc cccgacctgg cggcgacgac gcggctgctc
      541 gccgtcctca cgccggtccg cgaacgggcg agctccacga tctggccacg cgagccagac
      601 ctctacggcc acgggtgcgg tcccgtacgg cgggcgggtt gcggcggcat tcaactacgc
      661 ccttgtcacg ctctacccga gggacccgct ggccagatgt gctgctcgtc ggtcggctct
      721 tgcgctgact gaccacgacg ctgcataaag gctgctgtcg cttcgtcgtg acgcccgcgt
      781 cttgccgacc tgccgatggc cgtttgacac ttggcgggga cggcggagcg gagtctgagt
      841 cggttcggtg gaggtagccc gatccgtgca aacgttcagg cgcgcgacca cggtcgggga
      901 gctcaccctt gctacaagat ccggacgaat ggctgttagt catcgaccgt gacccggttt
      961 cgggagtgga gggcagatat tcacacgacc gtcccgcgtg ggtgtaggtt tgcgcacgcc
     1021 gcgactaggt ttgcgcacgc cgcgaccggc gccgtccacg ccgttagggg aatggacggt
     1081 ctagatcccg ttgagtcaaa tccgaggaga gccagtcgat gagagtttcc tgcgttgcca
     1141 gtgatgcgcc atttcccgtc gaggcgagct tcaggagcga ggggagcgcg ctgctcatcc
     1201 agaagatccc tgcccggacg catgcctgca cggctatcac gcacaccgag ggagagtgtg
     1261 atgtctgagg atacgcagcg cagcctgagc cccgcggcgg ctcgtccgct ggcgacgacg
     1321 accaagacgg tcccgcagat ggaggcgatc acgcctcgct acctgttgcg ggcgctgccc
     1381 tgggtggagg tcgaggcggg tgcctaccgg gtcaaccgtc accgcagctt caccgtcggc
     1441 gacgggctta tcggcacgga gttcgacggc gacgacgcgc gcgtgttctc cgaggatctg
     1501 cgcgagatgg cgctcctcac cgacgcggac tcggcgctgc tgtcggccct cggcgagtcc
     1561 ttcggtcagg agacgttcga gcccggcgcg atcatcaccg agcccggcac cgacgccgac
     1621 cggctgtacg tgatcgccca tggcaaggta gagcgatggg gtgcgacgaa gtacggggag
     1681 gaagcgctga tcgacgtgct cgccgagggt gactacttcg acggcgaagc ctgggtcggc
     1741 gagcagcagt ggcgccatcg cgtcaaggcc gtgacgcact gcgtgctgct cgccctggaa
     1801 cgcgcgcggc tcaccgagct cgtcgcgcgc gaccagcagc tggctgcgcg gttcaaggag
     1861 cttgccgcca gcaacgggcg acggccgggc gacagcccga tcgatctcat gtcggggcac
     1921 gccggcgagc ccgagctgcc gcagaccttc gtcgactacg aggaggtccc gcgcgagtac
     1981 gagatgagca tcgtgcagac cgtgctgcgc gttcataccc gcgtgtcgga cctctacaac
     2041 gagccgatga gccaggtgac gcagcagacc cgcctgacga tggaggccct gcgcgagcag
     2101 caggagcgcc agatgctcca tcacccggac tacgggttgt tgaacaacgt ctccgtcaag
     2161 cagcgcgtgc gcacccgctc cgggccgccc acgccggatg acctcgacga gctgctgacg
     2221 cgcgtctgga agcggccggc gttcttcctc gcgcatccgc gtgcgattgc ggcgttcggc
     2281 cgcgagtgca cccgccgagg cgtgccaccg gcgatcatgg acgtctacgg cagcccgaag
     2341 ctcacgtggc gcggcgtgcc gctgcttcct agcgacaagc tcgacgtcaa cggcaccacc
     2401 gacggctcgg tcggcaccac gagcatcgtg ctcatgcgcg tcggcgagga cgagcgcggc
     2461 gtcgtcgggc tgcgcccggc ggccgtcccg gacgaggtcg agccgggcat ggcggtgcgc
     2521 gccatgggcg tcgaccggcg ggcgctgacg tcgtacctgc tgaccgccta cttctccgtc
     2581 gcggtgctcg tcaacgacgc gatcgccgtg ctcgagaacg tggacgtcgc caagtaccac
     2641 gactatgact gagacgaggt actggctcgc cgaccccccg gtggagcccg agcagccgcg
     2701 caagcagccg gcgtttgcag tc
//
LOCUS       DASVEU010000250         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1734589,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000250 DASVEU010000000
VERSION     DASVEU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734589"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF225_10600"
     CDS             <1..397
                     /locus_tag="VF225_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145744.1"
                     /translation="DHPSPSGNAMLAHVLLRLGRIYGDDELERQALGVLRLVRSALER
                     APSAFGWSLCALDLWLSPPRELAIVGPIDSPVARAALAPWQPNAVVAVGPAAGVPLLE
                     GKSLVDGKAAVYVCERFACQAPVTDPALL"
     gene            361..1293
                     /locus_tag="VF225_10605"
     CDS             361..1293
                     /locus_tag="VF225_10605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145745.1"
                     /translation="MPGARDGSRSALTSRAYVGADDLDALHAVVRAVWAVDKSHASLH
                     VGDVTWGMYQHEPELARPRRAEIFERDGEPVAFGILWLPQTLQSTVHPEHRDDAVYDA
                     LLEWFAAAAGAELDGGLDAELLESDVVFATALERHGYAPLGNDDWLEHRVRSLAEPIE
                     PSPLPGGYRLRNVEGEADVERRVAVHRAAFHPSKVTVSAYRRLRALPPYREQLDLVVE
                     APGGSFAAYALVWLDEENGVGELEPVGTHPKHQRLGLGKAVCVEACRRLREAGADTAI
                     VYSVHGYHAGKLYESAGFEVVDRHLQYRLATQEF"
     gene            1395..>2430
                     /locus_tag="VF225_10610"
     CDS             1395..>2430
                     /locus_tag="VF225_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7145746.1"
                     /translation="MRRVSLALLALVGALALAIPAAFAKADAKAADPGITARTITIGG
                     TFPLTGPASSYAPIPASMKAYFSYINARRGPDGKRGIYGRQIVFKYYDDGYNPVNSVQ
                     LARKSVQEDKVFAIVGTLGTEVNQAVQPYLNSARVPHVLVSTGASDFGKDYKKYPWTI
                     GWQPDYVSEGRLYGLDLAQKPNAKIAILYQNDSYGKDYLAGFKSALGTAKVRQQVVGE
                     EAFEVTAPSPASQIAKLRASGANTLVIFVTPRPTIQTYAIIRALGWKPAQIYVNSVSA
                     TDTFMTTAVGASSAATVNGSISAAYLKDLASPTWANDPIVKQYMTLMAKYNPRGRVTD
                     GLNFYGFAKAD"
BASE COUNT          386 a          820 c          819 g          405 t
ORIGIN      
        1 ggatcatccg tcgccgtcgg gcaatgccat gctcgcccac gtcctcctgc ggctggggcg
       61 gatctacggc gacgacgagc tcgagcgcca ggcgctcggt gtcctccgac tcgtccgatc
      121 tgcgctcgag cgcgctccct cggccttcgg ctggtcgctc tgcgcgctcg acctctggct
      181 cagcccgccg cgcgagctgg cgatcgtcgg gcctatcgat tcgccggtcg cccgggcggc
      241 gctcgctcca tggcagccga acgcagttgt cgccgtcggc cccgcagccg gcgtgccact
      301 gctcgagggc aagagtctcg tggacgggaa ggcggccgta tacgtttgcg agcgattcgc
      361 atgccaggcg cccgtgacgg atcccgctct gctctgacct cgcgcgcgta cgtgggcgcc
      421 gacgacctcg acgcgttgca cgccgtcgtg cgcgcagtct gggcggtcga caagtcgcat
      481 gcgtcgctcc acgtgggcga cgtcacctgg ggcatgtacc agcacgagcc cgagctcgcc
      541 cggcccaggc gggcggagat cttcgagcgc gacggcgaac cggtcgcgtt cgggatcctc
      601 tggctgcctc agaccctcca gagcaccgtc catccggagc atcgcgacga cgcggtctac
      661 gacgccctgt tggagtggtt cgcggcagcg gccggcgcgg agctggacgg cggcctggac
      721 gcagagctcc tggagagcga cgtcgtcttc gctacggccc tcgagcgcca cggttacgcg
      781 ccgctcggca acgacgactg gctggagcat cgcgttcggt cgctcgccga gccgatcgag
      841 ccgtcgccgc ttcccggggg gtaccgcctc cgtaacgtcg agggcgaggc cgatgtcgag
      901 cgccgcgtcg cggtccatcg cgcagcgttt catccgtcga aggtcacagt ctctgcgtac
      961 cggcgcctgc gggcactgcc gccctaccga gagcagctcg atctcgtcgt cgaggcgccc
     1021 ggcggaagct tcgcggcgta cgcgctcgtc tggctggacg aggagaacgg ggtcggcgag
     1081 ctggagccgg tcggcaccca cccgaagcac cagcggctgg ggctcgggaa ggcggtctgc
     1141 gtggaggctt gccggcggtt acgggaggcc ggagccgaca ccgcaatcgt ctattccgtc
     1201 cacggctacc acgccgggaa gctgtacgag tcggccggct tcgaggtggt cgaccggcac
     1261 ctgcagtacc gtctggctac acaggagttt taaggctaac ttcgtcgtcc agccgttgtc
     1321 ttaccctttg gaactgcgta aagtcgccac gcgcgactct ttttttctct gtctgttctc
     1381 caggaggagt gttcatgcgt agggtctcac tggctttgct ggctctcgtc ggggcgctcg
     1441 ccctggcgat tccggctgcg tttgcgaagg cggacgccaa ggcggccgat ccggggatca
     1501 cagcccggac gatcacgatc ggcggcacct ttccgctgac gggaccggca tcgagctatg
     1561 cgccgattcc ggcgtccatg aaggcgtact tcagctacat caacgcgcgg cgcgggcccg
     1621 acggcaagcg cggcatctac ggacggcaga tcgtcttcaa gtactacgac gacggctaca
     1681 acccggtcaa ctcagtccag ctcgcgcgga agtccgtgca ggaggacaag gtcttcgcca
     1741 tcgtcggcac gctcggcacc gaggtgaacc aggctgtgca gccgtatctg aactcggccc
     1801 gggtgccgca cgtcctcgtc tccaccggag cgagcgactt cggcaaggac tacaagaagt
     1861 acccgtggac gatcggctgg cagccggact acgtctccga gggtcggctc tacggcctcg
     1921 acctcgcgca gaagccgaac gcgaagatcg cgatcctcta ccagaacgac agctacggca
     1981 aggactacct tgcaggcttc aagtcggcgc tcggaactgc gaaggtgagg cagcaggtcg
     2041 tcggtgagga ggcgttcgag gtcacggcgc cgagcccggc ctcgcagatt gcgaagctgc
     2101 gggctagcgg ggcgaacacc ctggtcatct tcgtgacgcc gcggccgacg atccagacct
     2161 acgcgatcat ccgggcgctc ggctggaagc cggcgcagat ctacgtcaac tcggtctcgg
     2221 caaccgacac gttcatgacg acggccgtcg gcgcatcgag cgcggccacc gtgaacggct
     2281 cgatcagtgc cgcgtatctg aaggatctgg cgtcgccgac ctgggccaac gaccccatcg
     2341 tgaagcagta catgacgttg atggccaagt acaacccgcg tggcagggtc accgatgggc
     2401 tcaacttcta cggcttcgcc aaggcggaca
//
LOCUS       DASVEU010000251         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_180363,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000251 DASVEU010000000
VERSION     DASVEU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180363"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF225_10615"
     CDS             <1..503
                     /locus_tag="VF225_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX7145747.1"
                     /translation="DYTRGACPGCTNLGDGLDGSLVHLNHRDVTLLCFSRAPIERLTA
                     YKQRMGWEFPYVSTYNSDFAFDFGLVLTGEQAQQIPEVKEMIDNPSEWLQEWSGQIGA
                     EPKDGLREAPSWIAFARENGTVYHTYTVMAPDPFVQPYFSFLLERTPKAQPSEPRAWR
                     KDEYPD"
     gene            complement(455..1726)
                     /locus_tag="VF225_10620"
     CDS             complement(455..1726)
                     /locus_tag="VF225_10620"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7145748.1"
                     /translation="MTAPLPDRMLSIGADPRDTADERFRKRLLVGVALVILPVAFLWG
                     CLYWVVGERAVALTPWAYVAGSTISLVVFARTGNFALLRTAQQVLILVAPAAGTVMLG
                     GLDESSSVILWSLFAPLGAVAFDRPSRAWPWFAAFAATILVALALSEVVRPDGADLPE
                     AFVRTFDVLNIVVVSFVAMVLLVTFARGRDAAQARVETLLLNVLPADIAQRLQSDPNA
                     IADHFDEASVLFADVVDFTPLSSRLDAREVVGLLDRLFTSFDELVDRYEVEKIKTIGD
                     CYMVAAGVPRRRPDHAHALALLALELRECAKTCLPDGRDHDLRLRIGISSGPVVAGVI
                     GRRRFLYDLWGDTVNMASRMESHGTPDEIQITGSTWQLVRDDFVTEPIGLVDVKGKGE
                     VETWRLVGPRESLADPQSGYSSFRQARGSEG"
     gene            complement(1771..2364)
                     /locus_tag="VF225_10625"
     CDS             complement(1771..2364)
                     /locus_tag="VF225_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7145749.1"
                     /translation="MGKLVVTEFVSLDGVMQAPGGEDFKYPGWSFAFDRGEDGNQFKL
                     DETLETEALLIGRVTYESFAGAWPKREGEFADKFNTMPKYVVSSTLEDPEWNNTTVLK
                     GDAVDEATKLKEQIDGIVQTPGSLRLVQTLLEHDLVDKLHLMVFPVILGTGRRLFGET
                     TEKTDWRLSEAKPVGPDGVLVLIYERARDASSAGAAS"
BASE COUNT          455 a          896 c          859 g          410 t
ORIGIN      
        1 ccgactacac gcgcggcgcc tgccccggct gcacgaacct gggcgacggg ctcgacggct
       61 cgctcgtcca cctgaaccac cgcgacgtga cgctgctctg cttctcgcgg gcgccgatcg
      121 agaggctgac cgcctacaag cagcggatgg gctgggagtt cccctacgtc tctacctaca
      181 acagcgactt cgccttcgac ttcggcctcg tgctgaccgg ggagcaggcg cagcagatcc
      241 ctgaggtcaa ggagatgatc gacaacccgt ccgagtggct ccaggagtgg tcaggccaga
      301 tcggggccga gcccaaggac gggctgcgcg aggctccgag ctggatcgct ttcgcgcgcg
      361 agaacggaac cgtctaccac acctacacgg tgatggcgcc cgacccgttc gtccagccgt
      421 acttcagctt cctgctcgag cgaacgccga aagctcagcc ctccgaaccc cgcgcctggc
      481 ggaaggacga gtatccggac tgagggtctg cgagcgattc gcgagggccg acgaggcgcc
      541 acgtctcgac ctcgcccttg cccttcacgt cgacgagccc gatgggctcg gtgacgaagt
      601 cgtcgcgcac gagttgccag gtcgagcccg tgatctggat ctcgtccggt gtcccgtgcg
      661 actccatccg gctcgccatg ttcaccgtgt caccccacag gtcgtagagg aagcggcggc
      721 gcccgatcac gccggccacg accggccccg agctgatgcc gatgcgtagc cgcaggtcgt
      781 ggtctctgcc gtccgggagg caggtcttcg cgcactcgcg cagctcgagc gcgagcaacg
      841 ccagtgcgtg tgcgtgatcc ggccgccgcc tggggacgcc cgccgcgacc atgtagcagt
      901 cgccgatcgt cttgatcttc tcgacctcgt agcggtcgac gagctcgtcg aagctcgtga
      961 agagccgatc gaggagcccg accacctcgc gcgcgtcgag ccggctcgag agcggcgtga
     1021 agtcgacgac gtcggcgaag aggacgctcg cctcgtcgaa gtggtcggcg atagcgttcg
     1081 gatccgactg cagccgctgc gcgatgtcgg ccgggaggac gttgagcagc agcgtctcga
     1141 cgcgcgcctg tgcggcgtcc cgtcctcgcg cgaaggtcac gagcaacacc atcgcgacga
     1201 acgagaccac gacgatgttg aggacgtcga aggtacggac gaaggcctcc ggcaggtcgg
     1261 cgccgtccgg ccgaacgact tcggagagag caagcgcgac caggatcgtg gcagcaaatg
     1321 ccgcaaacca cggccaggcg cggctggggc ggtcgaatgc gactgctccg agcggcgcga
     1381 acagcgacca cagaatcacg gagctggact cgtcgaggcc accgagcatg accgtcccgg
     1441 ccgcgggggc gacgagaatc agaacctgct gcgccgtgcg caggagcgcg aaattcccgg
     1501 tccgcgcgaa gacgacgagg ctgattgtcg acccggcgac gtaggcccac ggcgtgagcg
     1561 caaccgcgcg ctcacccacg acccagtaga ggcagcccca gagaaacgcg accgggagga
     1621 tgacgagcgc cacgccgacg agcaggcgct tccggaagcg ctcgtcggcc gtgtcgcgcg
     1681 gatcggctcc gatggagagc atgcgatctg gaagcggcgc ggtcacgacc ggcgagcgta
     1741 gtaccacgcc agaacctcca cgttaggcgg tcagctggcg gcgccggccg aagaggcgtc
     1801 cctcgctcgc tcgtagatca ggaccagcac gccgtcgggc ccgaccggct tcgcctcgct
     1861 gagccgccag tccgtcttct cggtcgtctc gccgaacagc cgccgcccgg ttccgaggat
     1921 caccgggaac accatcaggt gaagcttgtc gactagatcg tgctccagca gcgtttgcac
     1981 gagccggagg cttcccggcg tctggacgat cccgtcgatc tgctccttga gcttcgtggc
     2041 ttcgtcgacg gcgtcgccct tgaggaccgt ggtgttgttc cactccggat cctcgagcgt
     2101 cgacgagacg acgtacttgg gcatcgtgtt gaacttgtcg gcgaattcgc cttcgcgttt
     2161 cggccacgcg ccggcgaagc tctcgtacgt cacgcgtccg atcaggagcg cctcggtctc
     2221 cagcgtctcg tcgagcttga actgattccc gtcctcgccg cggtcaaatg cgaagctcca
     2281 gcccggatac ttgaagtcct cgccgccagg cgcttgcatg acgccgtcga gcgacacgaa
     2341 ctcggtcaca accagcttgc ccatcgggtc tcctttgttg cgggttggga actatgacct
     2401 tcggaaggag ccgaactcat cgcagcgccg aactcgttat agtgaaccta atggttcagt
     2461 atcccaagac cgtcgaccgc gctttcgcag ccctcgccga cccgacccgc cgcgccgtcc
     2521 tcgaacggct cggttcgggc agcgcgacga tcagcgagct cgccgggccc ttcggcatgt
     2581 cgctgaccgg gatgaagaag cacatccggc tgctcgagga
//
LOCUS       DASVEU010000252         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1735405,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000252 DASVEU010000000
VERSION     DASVEU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735405"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF225_10630"
     CDS             complement(<1..325)
                     /locus_tag="VF225_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145750.1"
                     /translation="MGLFAFNLFLLVVGVALLFGARSWESWSDLFRLSGFAYMLGVAA
                     TGVALVIELVAGLDLSLLSILATGLGLAAAAVLAGRALGRSLPPSRVFSGHISLTTAI
                     FGALTI"
     gene            complement(340..801)
                     /locus_tag="VF225_10635"
     CDS             complement(340..801)
                     /locus_tag="VF225_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145751.1"
                     /translation="MIAAAREHVRKGGASAVIVVLCVALTLLVAGIRYVKALSQLGDT
                     ASSNSSLSFDDREIAGGNSIVVDQLAAYQARALIPVTDSYHVVVGPGLRGATELTKDH
                     IDGWLRYFLMPRRPEDDARWVICYGCDPATLGNRYEVRWRDDNGISIGYLP"
     gene            complement(798..2099)
                     /locus_tag="VF225_10640"
     CDS             complement(798..2099)
                     /locus_tag="VF225_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7145752.1"
                     /translation="MKFAGTLFLATLFCVTWEKVQWNVAGAVGLADVLTVMFLVAFAL
                     EWGRPRFPRTTVTVLAIFAALLVIYLIGFFNIETKQSLDQWGKGMVKFVLHFLFLAAG
                     VAYLARRSTDFYWKAVAALSAGLVVNGAYGVLQLLAARAGTNLDHVLLSPLTGGASSI
                     NVYGAIGGTAVYRPNALTTDPNHLGVMLCIPLLTLLPVYLRLERGHRLRVPVAVVLAF
                     LFLVMLSTLSRSGLLGLVVGLLVLAIPYRRRLVSRALLVPLGAVAAVLIAIVWSRRHY
                     FEVLLKSRVQTGGGSENAHFAVYDFVPQVIHSHPLFGLGLNTFSVYYEFVTGKSNWGP
                     HSFYVALIVETGLVGSVVFLLFLRYLFLRLHAARALGRQLALEGFGVARRVRPLAWGM
                     TAALAGTMAANFFYLTMQFYYFYAFAVLVLALPIVFGRSAR"
     gene            complement(2194..>2704)
                     /locus_tag="VF225_10645"
     CDS             complement(2194..>2704)
                     /locus_tag="VF225_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145753.1"
                     /translation="DPRPFLTALAESGLEDVVARFVGDFRVADREWAESLGLGNRLEL
                     HPYVPRRQSLALQRDSDALLLLIPEAGGRGQGVLSGKVFEYLAAERPILAAVPPDGAA
                     AALIRETDAGVVAAADDVPALKAALSELHVKWQAGALDGTPLSADDRERLGRAARVEE
                     LAEVLRGLE"
BASE COUNT          555 a          901 c          898 g          350 t
ORIGIN      
        1 cgatcgtcag cgctccgaag atcgcggttg tcaacgagat gtgtcctgaa aacaccctgc
       61 tcgggggcaa cgagcgtccg agcgcgcggc cggcgagaac cgcggcggcg gccaggccga
      121 gcccggtcgc aagaatcgag agaagtgaca gatcgagccc ggcgacgagc tcgatcacaa
      181 gagctacccc cgtcgccgcg accccgagca tgtaggcgaa gccggaaagg cgaaacaggt
      241 cgctccatga ctcccagctc cgggctccga agagcagagc gacaccgacg acgagcagga
      301 agagattgaa agcgaacagt cccacgagcg agtgcgcgtt cacggtaggt acccgatcga
      361 gatgccgttg tcatcccgcc aacggacctc gtagcgattc ccgagcgtgg cagggtcgca
      421 gccgtagcag atcacccagc gtgcatcatc ctcaggccgg cgaggcatca ggaagtacct
      481 gagccacccg tcgatgtggt ccttggtgag ctccgttgcg ccgcgaagac ccggtccgac
      541 aacgacgtgg taggagtcgg tcacaggaat cagggcacgc gcctggtagg cggcgagctg
      601 atcgacgacg atgctgttgc cgccggcgat ctctcggtcg tcgaacgaga gcgagctgtt
      661 gctggacgcc gtgtcgccaa gctgcgacag ggctttcaca taccgaatcc ctgcgacaag
      721 gagcgtcagc gcaacgcaga ggacgacgat gacggccgag gcgccaccct ttcgcacgtg
      781 ctctcgggct gccgcaatca tcgtgcgctc cggccgaaga cgatcggtaa cgcgagcacg
      841 agcaccgcga atgcgtagaa gtaatagaac tgcatcgtca ggtagaagaa gttcgccgcc
      901 attgtcccgg cgagcgcggc ggtcatcccc caggccagcg gccgcacgcg gcgtgcgacc
      961 ccgaatccct ccagtgccaa ctgccggccc aacgcccgcg ccgcgtgcaa acgcagaaac
     1021 agatagcgca ggaagagcag gaacaccacc gacccgacta gccctgtctc gacgatcagc
     1081 gcgacgtaga acgaatgcgg cccccagttc gacttgccgg tgacgaattc gtagtagacc
     1141 gagaaggtgt tcagccccag gccgaacagc gggtgtgagt ggatgacttg cggcacgaag
     1201 tcgtagacgg cgaagtgggc attttccgaa ccgccgcccg tctgcactcg tgatttcagc
     1261 agcacctcga agtagtgacg gcgactccac acgatggcaa tgaggaccgc ggcgacggca
     1321 ccgagcggca cgagcagcgc acgcgacacg agacggcgcc gataagggat cgcgagcacg
     1381 agcagtccga ctacgagccc gagcaacccg ctccgcgaga gcgtcgagag catcacgagg
     1441 aagagaaagg cgagcacgac ggcgaccgga acgcgcaagc ggtgccctcg ctccagtcgc
     1501 agatagacgg gcaacagcgt cagcaggggg atgcagagca tcaccccgag atggttggga
     1561 tcggtcgtga gcgcgttcgg ccggtaaacg gccgtcccgc cgatcgcgcc gtagacgttg
     1621 atcgagctgg ccccgcccgt cagcggcgag agcagaacgt gatccagatt cgtgccggct
     1681 cgcgcggcga gcagctgcag cacgccgtag gcaccgttga cgacgagccc ggcgctgagc
     1741 gcggcgaccg ccttccagta gaagtccgtg gaacggcggg ccagatacgc gacacctgcg
     1801 gccaggaaga ggaagtggag gacgaacttc accatgccct tgccccactg gtcgagcgac
     1861 tgcttcgtct cgatgttgaa gaagccgatc aggtagatca cgagcagcgc cgcgaagatg
     1921 gcgagcaccg tgactgtggt gcggggaaag cgcggtcggc cccactcgag cgcgaacgcg
     1981 acgaggaaca tcacggtcaa cacgtccgcg aggcccactg cgcccgctac gttccactgg
     2041 accttctccc acgtgacgca gaacagcgtc gcgaggaaga gcgtgcccgc gaacttcaca
     2101 gcgtgagctc cgatccgcgc ggttttggcc agcggcgcag acggacgggc gggcaagccc
     2161 acccgtcctg aggccggcac cgcaagcggt gcctcattcg agcccccgta gcacctcggc
     2221 aagctcctcg acccgcgcgg cccggccgag gcgctcccgg tcgtccgccg acaggggcgt
     2281 tccgtccagc gcgccggcct gccacttcac gtgaagctcg gacaaagctg ccttcagggc
     2341 aggaacgtcg tccgccgcgg cgacgacacc ggcgtcggtt tcgcggatca gtgccgccgc
     2401 cgcgccgtcg ggcggcacgg cggccaggat tggacgttcg gccgcgaggt actcgaagac
     2461 cttgcccgac agcactccct gaccgcgtcc accggcctcc gggatgagca gcagcagggc
     2521 gtcggagtct cgctgcaacg cgagcgactg ccggcgcggc acgtacggat gcagctcgag
     2581 cctgttcccg agcccgagcg actccgccca ctcgcgatcg gcaacgcgga agtcgccgac
     2641 gaagcgtgcg acgacatcct ccagccccga ctccgccagc gcggtcagaa acggcctcgg
     2701 atcg
//
LOCUS       DASVEU010000253         8896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1736281,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000253 DASVEU010000000
VERSION     DASVEU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736281"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VF225_10650"
     CDS             complement(<1..1137)
                     /locus_tag="VF225_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145754.1"
                     /translation="MATIEERTAGGVSFGLTHEQRALRDLAHDFAEREIRPREREYDE
                     GSIHPADVIAKAHELGLMNVHIPEAYGGLELGAFEGMLIGEELCWGCSGIGTAIAANG
                     LGHGPVIIAGTEEQKREWLPPLVDDVLLTSFALTEPNAGSDVSGIQTTAVRDGDEYIL
                     NGSKMFITNAGHAAWFTVFASTDKAQGHRGLTAFVVPADLGGVVVDKHLDKMGQRSTD
                     TSALSFTDVRVPAANRLGEEGEGFKIAMKTLDFTRPGTAIGAVGVARAAFEYAAQYSK
                     ERVQFGMPIAMNQGINFMIADMATKIEASRLLCWQAAWMIDQGMRATLQSSYAKRFAN
                     EDWSVARSPWSCSQAACQTRRRAASISVAMSAIMKLTPWCIAIGT"
     gene            complement(1165..2022)
                     /locus_tag="VF225_10655"
     CDS             complement(1165..2022)
                     /locus_tag="VF225_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7145755.1"
                     /translation="MGVAAVITMATMLGLTPQQKAALVVVSGLPAPPGVGGVIVQGYD
                     RDAARPSGALVFVDQEGGKVKTFPRLPPRSSARAVRSKAEAFESGAATGRALGRAGVD
                     VDLAPVLDLPDGPLGSRQFRSPPFGVAFARGLAAGGAGSCVKHFPGLGSLRFSTDEHP
                     YVVGRVRDADLAPYRAAVEAGVSCVMVGHGVYPTFGPRRAALEPAAYRLLRDLGFEGV
                     AITDSLDVVRGHAQDWAVAAARAGADLLLFTSGRGAARAIRVLLPLARSGELDAHVTR
                     VLRFRLAID"
     gene            2045..3268
                     /locus_tag="VF225_10660"
     CDS             2045..3268
                     /locus_tag="VF225_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7145756.1"
                     /translation="MDFELSDEQRLLQDTVRRFVDDRILPNAVENDINHYLDLGAIEG
                     MAELGILGIVIPEEYGGAGLDFVSEALACEEIERGEAAFRTLISVHVGLNSLALLKYA
                     SEEQKQRWLVPQASGEKLACFGLTEPAAGSDVAAMKSTARREGDVYVLNGSKNWISYA
                     NVADHALVFAKTDPAAKHKGISAFVIERGMPGFTTADTEHKLGIWAGSTGELFFQNLE
                     VPAENMIGEEGQGFEIAMYGLDQGRFTVAAGAVGVIRACLERSTEYARERETFGEPIG
                     KYQFVQDMIAEMVLGYETSKLLVMQAAWMKDQGMRNTRETSLAKWHATEAAFRAAHLA
                     IQVHGAYGYSAEYGIERYFRNARAPIIYEGTTQIHKMMQAEHALGFRALNGRDGDVSP
                     LCSWAPALSPVHARG"
     gene            complement(3280..3903)
                     /locus_tag="VF225_10665"
     CDS             complement(3280..3903)
                     /locus_tag="VF225_10665"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX7145757.1"
                     /translation="MLGQEGGLMDWDLNGTVVIACNCDYGCPCNFNALPTQGKCEGSW
                     TWHIDRGAFGNTALDDLNFTVAVNWPGAIHEGNGEAVILVDERADQAQRDAIAALVSG
                     DVGGPWGVLAWTWPTVHGPKPVPYDVQLDGVNSRVKAGASFEVVSETIKNPVTGAEVH
                     PGARLPEGIVFKEGDFGSSTTYRLSDEVAFEHPGKYTAVGPFDYSGP"
     gene            complement(3927..4931)
                     /locus_tag="VF225_10670"
     CDS             complement(3927..4931)
                     /locus_tag="VF225_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145758.1"
                     /translation="MALWDELRGLALNIEDFGTERKSVDVSTQFTRVTTTVVLGGEGE
                     EGRGEDVTYTGEDHDWFPDLAPPGRTTLGELSAQLDGLRLFEAEPKMGASADYRRWAF
                     ESAALDLALRQNDLSLGAAVGREYRPLRFVVSTRGDAFAWLQHNPELELKLDPESDWD
                     RPFMERLAATGRVRVLDFKAYYTGTPVDVVPDPVLYRAAVELFPAVVLEDASLDGECG
                     AALQGHEPRLSFDAPIHSVADVRALPVEPGWLNIKPSRFGTIERLLECIEHCAANRIQ
                     MYGGGQFELGVGRRHIQVLASLFYPEGPNDVAPREYNMGEPRAGLPQSPLPPPDEVGF
                     "
     gene            complement(4931..6202)
                     /locus_tag="VF225_10675"
     CDS             complement(4931..6202)
                     /locus_tag="VF225_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7145759.1"
                     /translation="MTQLTAEAAVSSQTVRRSLFWTLGFGAFGLAFSITTTAAYLPPL
                     LHRFTESGALIGLVLGAEGVFALMLSPVIGPWSDTFHTPMGRRRPFMLAALGPMGFCL
                     LLMPFMPNLWTTTLIVLAFFFAYYVYEPPYRGLYPDLLPPSTYGRAQGIQHVLRGIAL
                     GAALVGGGFLLKVWQAAPFVLAAFVTTAACAVPIIFVREDGGHGRVFEGVGTYFKRSW
                     AILREEAEVRRFLLANAAWEGTFAAARTFVVLYVLDGLHESKTTSSTLLGAVAAGYVI
                     AALIAGRLGDRFGIARVIFFSSFVYGGGFLVAGTATTWHNWYFALIVPVAVAGGMVMT
                     LAWGLLFKLMPQEHRGAISGLATTTKGLGLLLGAPVAGVAIDLARPYLHATNGYQILW
                     PVCGIPILAAIPLVYQLMRVEPTGKPEPTLG"
     gene            complement(6202..6819)
                     /locus_tag="VF225_10680"
     CDS             complement(6202..6819)
                     /locus_tag="VF225_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7145760.1"
                     /translation="MTDRSTGQEDKRRLILDAAVRVFAHKGYHTSRVGDIAEEAGVAH
                     GLLYHYFRSKEELLETIFRETWRDVLDAVRSVEETDECARDQLAGIAKILLRAWRRDP
                     DLVRVLVREVTRSSHLQQRIGEIEAAFDGIERVIVHGQQEGEFRSDLDPRMASYVFYG
                     ALEEILTGWVLGQLEDGDEAIARAEETVVEVICGGLAVDRETAKV"
     gene            6869..7825
                     /locus_tag="VF225_10685"
     CDS             6869..7825
                     /locus_tag="VF225_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7145761.1"
                     /translation="MVLRGLLVVDFTRYLPGPFASRELLRLGARVVRVETPDGDPLRS
                     FAPGWDAALNAGKESVVWDLERDPGLGPALAARADVVLEGFRPGIADRLGVGAAALPK
                     SLVYCSVTGFGAGSLRAGHDLNYAGWAGLLADTAPALPPVQIADLAGGSLFAVIEILA
                     ALLERQRSGTGAHIVVSMTHNAHRLAAHRLRGEPERTLTGGLACYRTYETADGRFLTV
                     TAVESKFWQRLCELIGRPELVERHYQEDQEALAMELATVFRSRPLSEWLDLFEGEDVM
                     AGPVATLAEASEWLGEAVASGSAAALGEHTERWRADLASFEG"
     gene            7860..8693
                     /locus_tag="VF225_10690"
     CDS             7860..8693
                     /locus_tag="VF225_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145762.1"
                     /translation="MTPGRVLSLLCFALCASALAGCAGGDALALDPVARAANTTAQTT
                     SSRFEFRASLSGSSVGSLSFNGNGVYDGKNKSGWMNMHFALPPTAQAQIGSTDPSMEM
                     IFDGSHGLVMYMRSPLFNKIVPTGKWVKMDLEKIAKKEGVDLGAIMNANQADPSQSLR
                     LLMASSNARVTGSERIRGVQTTHYAFSIDYKKLAHGNKAFEKLQEATGSISAPAEAWI
                     DAKGRVRRLAVAMSLGGQLATPMTLTMTEDLYDFGVRTNIMPPSDDLVVDLSSLVGAG
                     S"
     gene            complement(8690..>8896)
                     /locus_tag="VF225_10695"
     CDS             complement(8690..>8896)
                     /locus_tag="VF225_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145763.1"
                     /translation="QAKAIDTVFRAIHEGDADPKLLAYQYLQVLPQIAQGESNKVWII
                     PSEVTRALGQLTDALPRRSDQQES"
BASE COUNT         1504 a         3042 c         2974 g         1376 t
ORIGIN      
        1 cgtcccgatc gcgatgcacc agggggtcaa cttcatgatc gccgacatgg cgaccgagat
       61 cgaggcggcg cgcctcctcg tctggcaggc cgcctggctg cacgaccagg gcgagcgcgc
      121 gacgctccag tcctcgttcg cgaagcgctt tgcgtaggac gactgcagcg tcgcgcgcat
      181 tccctggtcg atcatccagg ctgcctgcca gcagagcagc cgtgatgcct cgatcttcgt
      241 cgccatgtcg gcgatcatga agttgatgcc ctggttcatc gcgatcggca tgccgaattg
      301 cacgcgctcc ttcgagtatt gagccgcgta ctcgaacgcc gcacgcgcga ctccaacggc
      361 accgatcgcg gtgcccggac gagtgaagtc gagcgtcttc atcgcgatct tgaagccctc
      421 accctcttcg ccgaggcggt tcgccgccgg cacacggacg tcggtgaacg agagcgccga
      481 cgtgtccgtc gagcgctggc ccatcttgtc gagatgcttg tccacgacga caccgccgag
      541 atccgccggg acgacgaacg ccgtcaggcc gcgatgaccc tgcgccttgt cggtcgaggc
      601 gaagaccgtg aaccaggccg catggccggc gttcgtgatg aacatcttcg acccgttgag
      661 gatgtactcg tcgccgtcgc gtaccgccgt cgtctgaatg cccgacacat cggagcccgc
      721 gttcggctcg gtcaacgcaa atgacgtgag cagcacgtcg tccacgagcg gcggcagcca
      781 ttcgcgcttc tgctcctccg tccccgcgat gatcaccggc ccgtgcccga gcccgtttgc
      841 cgcgatggcc gtgccgatgc cggaacaccc ccagcaaagc tcctcgccga tgagcatgcc
      901 ctcgaaggcg ccgagctcga ggccgccgta ggcctccgga atgtggacgt tcatcaggcc
      961 gagctcgtgg gccttcgcga tcacgtctgc ggggtggatc gagccctcgt cgtactcgcg
     1021 ctcgcgcggg cggatctcgc gctcggcgaa gtcgtgcgcc aggtcgcgca gcgccctctg
     1081 ctcgtgcgtc agcccgaacg agacgccgcc ggcggttcgt tcttcgatgg tcgccatagg
     1141 ctcaagggta cctcgactga ctggtcagtc aatcgccagt cggaaccgta ggacacgggt
     1201 cacatgggcg tccagctcgc cactacgggc gagcggaagg aggacgcgaa tcgcgcgcgc
     1261 cgcgccccgt ccgctcgtga acaggagcag atccgcgccg gccctggcag cggcgaccgc
     1321 ccagtcctgc gcatggccgc ggacgacgtc gagcgagtcc gtgatcgcga caccctcgaa
     1381 cccgaggtcg cgcaagagac ggtatgcagc cggctcgagc gccgctcggc gcgggccgaa
     1441 agtcggatag acgccgtgac cgaccatcac gcacgacacg cccgcctcga ccgcagcacg
     1501 gtacggcgca aggtcggcat cgcggactcg accgacgacg tacggatgct catccgtcga
     1561 aaagcgcagc gagccgaggc ccgggaagtg cttgacgcac gagccggcgc cgcccgccgc
     1621 tagcccgcgc gcaaacgcga cgccgaaggg cggggagcga aactgccgcg atccgagcgg
     1681 gccgtccggg aggtcgagca ccggggcaag gtcgacgtcg acgcccgcgc ggcccagcgc
     1741 acgccccgtc gccgcgcccg actcgaaggc ctctgccttg ctccgcaccg cgcgggccga
     1801 cgaccgcggc ggcaggcgcg gaaaggtctt caccttccca ccctcttggt cgacgaagac
     1861 gagcgcgccg gacggcctgg ccgcgtcgcg atcgtagccc tgcacgatca cgccgcccac
     1921 acccggagga gccggtagcc cggagacgac gacgagggcc gccttctgct gcggcgtcaa
     1981 ccccagcatc gtggccatgg tgatgacggc ggcaacgccc aaagcctccg tagactaggc
     2041 cgtcatggac ttcgagctca gcgacgagca acggctgctc caggacacgg ttcggcgctt
     2101 cgtggacgac cggatcctgc ccaacgccgt cgagaacgac atcaaccact acctcgacct
     2161 gggggcgatc gaagggatgg ccgagctcgg gatcctcggg atcgtgattc cggaggagta
     2221 cggcggcgcg gggctcgact tcgtctccga ggcgctcgcg tgcgaggaga tcgagcgcgg
     2281 cgaggcggcc ttccggacgc tgatctccgt ccacgtcggg ctcaactcgc tcgcactgct
     2341 gaagtacgcg tccgaggaac agaagcagcg ctggctcgtc ccgcaggcga gcggcgaaaa
     2401 gctcgcgtgc ttcggcctga ccgagcccgc cgcgggctcc gacgtcgccg cgatgaagtc
     2461 gacggcgcgg cgcgagggcg acgtctacgt cctcaacggc tcgaagaact ggatctcgta
     2521 cgcgaacgtg gccgaccacg cgctcgtgtt cgcgaagacg gatcccgctg cgaaacacaa
     2581 ggggatctcc gcattcgtga tcgagcgcgg catgccgggc ttcactacgg cggacacgga
     2641 gcacaagctc ggcatctggg ccggctccac gggcgagctc ttcttccaga acctagaggt
     2701 tccggcggag aacatgatcg gcgaagaggg ccagggcttc gagatcgcga tgtacggcct
     2761 cgaccagggc cgcttcacgg tcgccgccgg cgcggtcggc gtcattcgtg cgtgcctcga
     2821 gcgctccacc gagtacgcgc gcgagcgaga gacattcggc gagccgatcg ggaaatacca
     2881 gttcgtgcag gacatgatcg ccgagatggt gctcggctac gagacgtcga agctgctcgt
     2941 catgcaggcg gcctggatga aggaccaggg aatgcggaac acgcgcgaga cgtcactggc
     3001 gaagtggcac gcgaccgaag ccgcgttccg cgcggcccat ctcgccatcc aggtgcacgg
     3061 cgcctacggc tattccgcgg agtacgggat cgagcggtac ttccgcaacg cgcgcgcacc
     3121 gatcatctac gaggggacga cgcagatcca caagatgatg caggccgagc acgcgcttgg
     3181 cttccgcgcc ctgaacggcc gtgacggcga cgtgtcgccg ttgtgctcgt gggcacccgc
     3241 actgtcgccc gttcacgcgc gcgggtagta agtacgccgc tagggcccgc tgtagtcgaa
     3301 cgggccgacc gcggtgtact tcccgggatg ctcgaaggcg acctcgtccg acaggcgata
     3361 cgtcgtcgac gagccgaagt cgccctcctt gaacacgatt ccctcgggaa gccgcgcgcc
     3421 cgggtgcacc tccgctccgg ttaccgggtt cttgatcgtc tcggacacga cttcgaacga
     3481 agcgcccgcc ttcacgcgtg agttcacgcc gtcgagttga acgtcgtagg gcaccggctt
     3541 gggcccgtgg acagtcggcc aggtccaggc gagcaccccc cagggccctc cgacatcgcc
     3601 actgacgagc gccgcgatcg cgtcgcgctg cgcctggtcg gcgcgctcgt cgaccaggat
     3661 caccgcctcg ccgttgccct cgtggatcgc gccgggccag ttcacggcga ctgtgaagtt
     3721 cagatcgtcg agcgcggtgt ttccgaaagc accacgatcg atgtgccacg tccagctccc
     3781 ctcacacttc ccttgcgtcg ggagagcatt gaagttgcac ggacagccgt agtcgcagtt
     3841 acacgcaatg acgaccgtcc cattgaggtc ccagtccatc aaccctcctt cctggccgag
     3901 cacgctagcg ccttcagcga tcgattctag aagccgacct cgtccggcgg cgggagtggg
     3961 ctctgcggca gtccggcgcg tggctcgccc atgttgtact cgcgcggcgc gacgtcgttc
     4021 gggccttcgg gatagaagag cgaggcaagc acctggatgt gccggcgccc gacaccgagc
     4081 tcgaactgtc cgccgccgta catctggatc ctgtttgcag cgcagtgctc gatgcactcc
     4141 aacagccgct cgatcgtgcc gaaacgcgac ggcttgatgt tgagccagcc gggctccacc
     4201 ggtagagcgc ggacgtcggc gaccgaatgg atcggcgcgt cgaagctcag cctcggctcg
     4261 tgcccttgca aggcggcgcc gcactcgccg tcgagggacg cgtcctccaa caccacagct
     4321 ggaaagagct cgacggcagc gcgatacagc acaggatccg gcacgacgtc gaccggcgtg
     4381 cccgtgtagt acgccttgaa gtcgaggacg cgaactcgac cagttgccgc gaggcgctcc
     4441 atgaacggcc tgtcccagtc gctctccgga tcgagcttca gctcgagctc cgggttgtgc
     4501 tgcaaccagg cgaaggcatc gccgcgcgtc gagacgacga aacggagcgg gcggtactcg
     4561 cgtccgacgg ccgcaccgag cgaaaggtcg ttctggcgca gcgccaggtc gagcgccgcg
     4621 ctctcgaagg cccagcgccg gtaatccgcg gatgccccca tcttcggctc cgcctcgaac
     4681 aggcgcaggc cgtccagttg cgccgagagc tccccgagcg ttgtgcgccc gggaggagcg
     4741 aggtcgggaa accagtcgtg atcctccccc gtgtaggtca cgtcctcgcc acggccttcc
     4801 tcgccttcac cgccgagcac gaccgtggtg gtgacgcgcg tgaactgagt cgagacgtcg
     4861 acggacttcc gttccgtgcc gaagtcctcg atgttcagcg cgaggccgcg cagctcgtcc
     4921 cagagcgcca tcagccgaga gtcggttccg gcttgccggt cggttctacg cgcatcagct
     4981 gatacacgag cgggatcgcg gcgaggatcg ggattccgca gaccggccac aggatctggt
     5041 agccgttcgt ggcgtgcagg tacggccgcg ccaggtcgat cgcaacaccg gccacgggtg
     5101 ctccgagcag aagaccgaga cccttggtcg tcgtcgcaag tcccgagatt gcgccgcggt
     5161 gctcctgcgg catcaacttg aagagcagcc cccaggccag cgtcatgacc atcccgcctg
     5221 cgaccgcgac gggaacgatc agcgcgaagt accagttgtg ccaggtcgtc gcggtgcccg
     5281 cgacaaggaa gccgccgccg tagacgaagg acgagaagaa gatcacgcgc gcgattccga
     5341 accggtcgcc gagccgccct gcaatcaggg cggcgatcac gtagccggcc gcgacggcgc
     5401 ccagcaacgt ggaagaggtc gtcttcgact cgtgcagccc gtcgaggacg tagaggacga
     5461 cgaacgtgcg ggccgctgcg aacgttcctt cccacgcggc gttcgcgaga aggaagcggc
     5521 ggacctccgc ctcttcgcga aggatggccc agcttcgctt gaagtaggtg ccgacccctt
     5581 cgaacactcg gccgtggccg ccgtcctcgc gcacgaagat gatcggcacc gcgcacgccg
     5641 ccgtcgtcac gaacgcggcc aacacgaagg gcgcagcctg ccagaccttg agcagaaaac
     5701 cgccgccgac aagggctgcg ccgagtgcga tcccgcgcag cacgtgctgg atcccctgcg
     5761 cccgcccgta cgtcgagggc ggcagcagat ccggatagag accgcggtac ggcggctcgt
     5821 agacgtagta cgcgaagaag aacgcgagga cgatcagggt cgtcgtccag agattcggca
     5881 tgaagggcat cagcagtagg cagaagccca tcgggccgag cgcggcgagc ataaaggggc
     5941 ggcggcggcc catcggcgtg tggaaggtgt cgctccacgg accgatcacg ggggagagca
     6001 tcagcgcgaa gactccttcc gcgccgagca cgagcccgat caacgcacct gactcggtga
     6061 agcggtgcag cagaggcggc aggtaggccg ccgtcgtcgt gatcgagaaa gcgaggccga
     6121 acgcgccgaa gccgagagtc cagaagaggc tgcgcctgac ggtctgcgag ctcaccgcag
     6181 cttcggccgt cagctgtgtc actagacctt ggctgtctcg cgatcgacgg ccaggccgcc
     6241 gcagatgacc tcgaccaccg tctcctcggc gcgagcaatc gcttcgtcgc catcctcgag
     6301 ctggccgagc acccaaccgg tgaggatttc ctcgagcgcg ccgtagaaca cgtaactggc
     6361 catacgcgga tcgaggtcgg agcggaactc gccttcctgc tggccgtgca cgatgacccg
     6421 ttcgatcccg tcgaatgctg cctcgatctc accgatgcgt tgctgaaggt gagagctgcg
     6481 cgtcacctcg cgcacgagca cgcgcacgag gtcgggatcg cggcgccatg cccgcagcag
     6541 gatcttcgcg atgccggcga gctggtcgcg cgcgcactcg tcggtctcct cgacggagcg
     6601 gacggcatcc agcacgtcgc gccacgtctc gcggaagatg gtctcgagca gctcctcctt
     6661 ggagcggaag tagtggtaca gcaggccgtg cgcgacgccc gcctcttccg cgatgtcacc
     6721 tacgcgagac gtgtggtagc ccttgtgggc aaacacgcgc acggcggcgt cgagaatgag
     6781 ccgacgcttg tcctcctggc ctgttgaacg gtcggtcaac gctccgagtg tagttgtagc
     6841 gacgcagtca gtggtagcgt cgcaggcagt ggtgctacgc ggcctgctgg tggtcgactt
     6901 cacgcgctac ctgcccggcc ccttcgccag ccgcgagctt ctgcggctcg gtgcgcgggt
     6961 cgtgcgggtg gaaacgccgg acggcgatcc gctccgttcg ttcgctcccg gctgggatgc
     7021 cgcgctcaat gcgggcaagg aatcggtggt ttgggatctc gagcgcgatc ccgggctcgg
     7081 gccggcgctc gccgcccgcg cagacgtcgt gctggagggc tttcgtcccg gcattgcaga
     7141 caggctcgga gtgggcgcgg cggcgctccc gaaatccctc gtgtactgct ccgtgacggg
     7201 cttcggtgcg gggagcctgc gcgccggtca cgacctcaat tacgccggct gggccggact
     7261 gctggctgac accgcgccgg cactcccgcc ggtgcagatt gccgacctcg ccggcggctc
     7321 gctcttcgcc gtcatcgaga tcctcgccgc cctgctcgag cggcaacgga gcgggaccgg
     7381 cgcgcacatc gtcgtctcga tgacgcacaa cgcccacagg ctcgccgccc atcgcctccg
     7441 tggggagccc gagcgcaccc tcactggcgg cctggcgtgt tatcgcacct acgagactgc
     7501 cgacggccgc ttcctcaccg tgactgcggt cgagtcgaag ttctggcagc gcctctgcga
     7561 gctgatcggc cggccggagc tcgtcgagcg gcactaccag gaggaccagg aggcgctggc
     7621 catggagctc gcgacggtgt tccggtcgcg gccgctctcg gagtggctcg acctcttcga
     7681 gggcgaagac gtgatggcgg gcccggtcgc aacgctcgcc gaggcgtcgg agtggctcgg
     7741 agaagcggtc gcatcgggca gcgcggccgc tctcggcgag cacacggagc ggtggcgcgc
     7801 cgacctcgcc tcattcgagg gataggcgcc ggcgcagccg aggacgattt gaatcgcgga
     7861 tgacccccgg ccgcgtcctc tccctgctct gcttcgcact ctgcgcctcg gcactcgcag
     7921 gttgcgccgg cggcgatgcg ctcgcgctcg acccggttgc gcgggccgcc aacacaaccg
     7981 cgcagacgac gtcatcgcgc ttcgagtttc gcgcctcgct ctccgggagc agcgtgggtt
     8041 cgctctcctt caacgggaac ggcgtctacg acggcaagaa caagtccggc tggatgaaca
     8101 tgcattttgc gctgccaccc acagctcagg cccagatcgg ctcgactgac ccgagcatgg
     8161 agatgatctt cgacggcagc catggtctcg tcatgtacat gcgctcgccg ctgttcaaca
     8221 agatcgtgcc taccggcaag tgggtgaaga tggatctcga gaagatcgcc aagaaggaag
     8281 gcgtggatct tggcgcgatc atgaacgcga accaggccga cccatcacag tcgcttcgcc
     8341 tgctgatggc ttcgagcaac gcgcgtgtga ccggatcgga acggatccgc ggcgtgcaga
     8401 cgacgcacta cgcgttcagc atcgactaca agaagcttgc acacggcaac aaggcgttcg
     8461 agaagctcca ggaagctacc ggctcgatct cggccccggc cgaggcgtgg atcgacgcca
     8521 agggccgcgt gcggcggctg gccgtggcga tgtcgctggg cggccaactc gcgacgccga
     8581 tgacgctgac gatgaccgag gacctctacg acttcggcgt gcgcacgaac atcatgccgc
     8641 cgtccgacga cctggtcgtc gacctctctt ctctcgtcgg cgctggttcc tagctctcct
     8701 gctggtcgct ccggcgcggc agcgcgtccg tgagctggcc gagcgcccgc gtgacctcgc
     8761 tcgggatgat ccacaccttg ttggattcgc cctgcgcgat ctgcggcagc acctgcagat
     8821 actggtaggc gaggagcttc gggtcggcgt cgccttcgtg gatcgcgcgg aagaccgtgt
     8881 cgattgcctt cgcctg
//
LOCUS       DASVEU010000254         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1736832,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000254 DASVEU010000000
VERSION     DASVEU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736832"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..486)
                     /locus_tag="VF225_10700"
     CDS             complement(76..486)
                     /locus_tag="VF225_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX7145764.1"
                     /translation="MDLDAAREFLRDHHRGVLATRRGDGGIQQSPVLVNVDGDGRAII
                     SSRETAYKVRNLRRDPWAQACVFTEEFFGRWIFFEGRAEVLSLPAAMEPLIDYYKRFP
                     DSNPDWDEYRARMERERRVLVRIELEQAGPDRQG"
     gene            complement(518..1078)
                     /locus_tag="VF225_10705"
     CDS             complement(518..1078)
                     /locus_tag="VF225_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7145765.1"
                     /translation="MATRTASDSRTNHTSPSGALRGRWSVISERSHVGFRVKKMGLYH
                     VKGRFKALDGTVVFAPGASAADILVDASSVTTRMPPRDVHLRSADFLDVKAYPVIAIS
                     ADSVTSVPDGELRVPATFEIRGHRRQVELVGHLHGAEQEEIVLHLAGELDRHDFGIRP
                     RQPFEMVVGRRVVLDVELVLAPDDGR"
     gene            complement(1130..1489)
                     /locus_tag="VF225_10710"
     CDS             complement(1130..1489)
                     /locus_tag="VF225_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX7145766.1"
                     /translation="MGLAARIAERIGGEASSGRAPGTRMRAVWNGAVLAESDRTVKLE
                     GNHYFPPDSLNREYFADSDRKTVCPWKGLASYYTVEVGGERNEGAAWTYRNPSPLARK
                     IKDHVAFWQGVQISPAD"
     gene            complement(1640..>2275)
                     /locus_tag="VF225_10715"
     CDS             complement(1640..>2275)
                     /locus_tag="VF225_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7145767.1"
                     /translation="LGTGMVGKTIATKLVSLGHEVTMGSRSAGNEEASGWASEAGDRA
                     RHGTFADAAGAGDIVVNCTAGARSLDALRAAGEPNLAGKVLIDVANPLDFSHGMPPTL
                     SVANTDSLGEQIQRAFPDAKVVKALNTMRCDVMVDPGRVPGEHDVFVCGDHEQAKREV
                     RELLRSFGWADDSVIDLGAISSARGTEGYLLLWLRLWEALGTADLNIKVVR"
BASE COUNT          327 a          774 c          789 g          385 t
ORIGIN      
        1 gtcgggacgt tccgggagat ctacagcggc gggctcgcgg cgctgaagca gaaggtcgag
       61 cagaacggga gctgatcagc cctggcggtc ggggcccgcc tgctcgagct cgatcctgac
      121 gaggacgcgc cgctcgcgct ccattcgcgc gcggtactcg tcccagtccg ggttcgagtc
      181 cgggaagcgc ttgtagtagt cgatcagcgg ctccatcgcc gccgggagcg ataggacctc
      241 ggctcgcccc tcgaagaaga tccagcgccc aaagaactct tcggtgaaga cgcacgcctg
      301 cgcccatggg tcgcggcgca ggtttcgcac cttgtacgcc gtctccctgc tcgagattat
      361 tgcccgaccg tcgccatcga cgttgacgag cacggggctc tgctggatgc cgccgtcacc
      421 gcggcgcgtc gcgagcacgc cgcggtggtg gtcgcggagg aactcgcgtg cagcgtcgag
      481 gtccacgcgg gcgactatgc catctttggg cacacggcta gcgtccgtcg tccggcgcca
      541 gcacgagttc gacgtcgagt acgacacgcc ggccgaccac catctcgaag ggctggcgtg
      601 gtcggatccc gaagtcgtgc cggtcaagct cgccggcgag gtggaggacg atctcctcct
      661 gctcggcgcc gtgcaggtgt cctacgagct cgacctggcg gcggtggccg cgaatctcga
      721 aggtcgcagg caccctgagc tcgccatccg ggacgctcgt gaccgagtcg gcgctgatcg
      781 cgatgacggg ataggccttc acgtcgagga agtcggcgct gcgcaggtga acgtcacgcg
      841 gcggcatacg ggtggtcacg gaactggcgt cgacgaggat gtcggcggcc gaagcgccgg
      901 gggcgaacac gacggtcccg tcgagcgcct tgaagcggcc cttcacgtgg tagagcccca
      961 tcttcttcac ccggaagccc acgtgcgagc gctccgagat cacgctccag cgtccgcgga
     1021 gggccccgct cgggctcgtg tggtttgtcc ggctgtcgga ggcggtcctg gttgccatgg
     1081 cgttcctttc gtcgctgtct cggttggacc cgcgcgcacg cgtgggggtt cagtcggcgg
     1141 gcgagatctg gacgccctgc cagaaggcga cgtggtcctt gatcttgcgc gcgagcgggc
     1201 tggggttgcg ataggtccac gcggcgccct cgttgcgctc gccgccgacc tcgaccgtgt
     1261 agtagctcgc gagtcccttc cacggacaga ccgtcttgcg gtcgctgtcc gcgaagtact
     1321 cgcggttcag cgagtcgggc gggaagtagt ggttgccctc gagcttgacg gtgcggtcgc
     1381 tctcggccag caccgcgccg ttccagacgg cccgcatccg cgtgccgggc gcgcgccccg
     1441 aggacgcttc accgccgatt cgctctgcga tacgcgctgc caatcccatt ctgtgtcctc
     1501 ctgttgtggt tcgtccgtca gcgttcgccg ccgacgtaca gatccgacac cgcggcggtc
     1561 gcagacgatt caccgcatgg ggacgggcgg cgcataagcg agagacggtg attcttcgtc
     1621 cctcccgccg ggacgaggct cagcggacca ccttgatgtt gagatcggcg gtgccgagcg
     1681 cctcccagag acgcagccag agcagcaggt atccctcggt cccgcgcgcg gaggagatag
     1741 cgcccaggtc gatgaccgag tcgtcagccc agccgaagct gcgcaggagc tcccggacct
     1801 cgcgtttcgc ctgctcgtga tcgccgcaca cgaagacgtc gtgctcgccc ggcacgcggc
     1861 ccggatcgac catcacgtcg caccgcatcg tgttcagtgc cttgaccacc ttcgcgtcgg
     1921 gaaaggcgcg ctgaatctgc tcgccgaggc tgtcggtgtt cgcaacggac agggtcggtg
     1981 gcatgccatg ggagaagtcg agcgggttcg cgacgtcgat gaggaccttg cccgccaggt
     2041 tcggctcgcc cgccgcgcgc agggcatcga gcgagcgcgc gccggcggtg cagttcacca
     2101 cgatgtcgcc ggcgcccgcg gcgtccgcga aggtcccgtg acgtgcgcgg tcgccagcct
     2161 ctgacgccca tccggacgcc tcctcgttgc cggcgctgcg cgagcccatc gtgacctcgt
     2221 gtccgaggct cacgagcttg gtcgcgatcg tcttgccgac catccccgtt ccgag
//
LOCUS       DASVEU010000255         6075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1542610,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000255 DASVEU010000000
VERSION     DASVEU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542610"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VF225_10720"
     CDS             complement(<1..535)
                     /locus_tag="VF225_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145768.1"
                     /translation="MPHTGHILHGRYAVVSCHVERPLDDEVWRRFSALQERRPGGFAI
                     AALMRPPAEGEDEERWLERARAASARAPFGLHTHWTAPDHARPTAGDPAALVRDQGSW
                     LESVGLRPTFFCGGGWYFDEGVAAAVADLGYADCTATAFRPGYLEPNAPHLQLDTPTL
                     LELGDNRRLLEVPTTHSI"
     gene            525..2366
                     /gene="asnB"
                     /locus_tag="VF225_10725"
     CDS             525..2366
                     /gene="asnB"
                     /locus_tag="VF225_10725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7145769.1"
                     /translation="MCGIAGLYSPAGPPNPELVDAMRAALVHRGPDEGSTDAFGHCVL
                     GHQRLKVIDLETGYQPVSNEAGDVITIFNGEAYNFLSLRDELRGHDVRGTGDTPILPH
                     LYEESGPRFVERLHGMFAVALWDAGRERLVLARDRLGKKPLLWTRLPDGTLAFASELK
                     ALLRLPHVSREIDLEAVDEYLSLQYVPGDRTALRGIHKLPPGHVLVAEGGSERIEPYW
                     KPESPAEPSASEPEWLERVRATVGEAVRKRLVADVPLGALLSGGIDSSIVVALMATAS
                     SQPVRTFTVGFPDARYDERAYARAVASRYATVHEEVEIEEDVASTLPRLAATFDEPLG
                     DEAAFPTFLIAEQARRHVTVALAGDGGDESFAGYERYIAHRLADRFPAPAARVGSAAL
                     RLLPGARREPRSPLFRAARFLSVAATPAGQRYARLMEVFPFELRRELWADARLAQQVR
                     LEPSQPGVAGLQLLDIETYLPGDLLLKADLASMAHSLELRSPFLDHEVVALGLALPES
                     LKTRGREGKVALRRAFAADLPLEVARRGKAGFGVPLGRWFRADLRDVAHDLLSNDRDW
                     FRPGTVRRLLDEHESGRADHGHRLWCLLMLELWVREHVEAPVLVTAA"
     gene            2363..3595
                     /locus_tag="VF225_10730"
     CDS             2363..3595
                     /locus_tag="VF225_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7145770.1"
                     /translation="MKRGQLWLIAVAAVAPRLAVLLHERGRILTAFTDKSDDFAQTFV
                     HHGTFGFIPGEPSAYTQPLYGFFLIPVYWIFGRTWWAVGGAQIVVATVTALLVYAIGA
                     RVLSRRAGAIAAILSTLNPYLVWHDVHLNREVLDQLVAAALILCTLVAADRRSVKWAS
                     LAGILGGLAVLGNVRLVFIPLVVAAYLVVRSGWSWAPVALVAVAALTVTPWVIRNKVE
                     VGCFALTTDGRALWKANNAQTYELLAHGKWIDDVKDPPNHPFPNPEEARDLYKQYGKK
                     YHVNECANQAYYEHKTWKFVRKHPGEKAKLAGQAVRMEWDPRTTASATDSGHGAIRSL
                     AQPLYTSLVYALALIGIFVTTRWFVGLALAVLAYQTLAAVVFVGATRYRIATDFLIAL
                     LAAGAIDWALTRWRRRQP"
     gene            3592..4677
                     /locus_tag="VF225_10735"
     CDS             3592..4677
                     /locus_tag="VF225_10735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145771.1"
                     /translation="MKILHVHRIGGIGGSERHLLTLLPALAAHGVDVRFLGLDDTTRA
                     PDPFYEALTVPYVRVPAPRDIDPRLALRVRRETRTADLVHTHLVHADVYGALGAKRLV
                     STKHNDDPFRAGAFRFVERALARRASKVIAITEALARFQVQRVGLPADKLEVIHYGLD
                     ELPQAWGSNPPDQVRPDARVLLAVCRLEPQKGVDVAVRALPDIRARHPQAELVVLGEG
                     PQRSELERLARELQVPVHLPGRVPDVASWLRRADLLVHPARWEGFGLALLEAMLAAKP
                     VVATNVSSIPEIVVDGQTGLLVAPDDAAALAAAVARLLDDPGNYGEQGRARAQSQFSV
                     ARMTDRTLALYETALHETGPRKTGPGV"
     gene            complement(4729..5832)
                     /locus_tag="VF225_10740"
     CDS             complement(4729..5832)
                     /locus_tag="VF225_10740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7145772.1"
                     /translation="MNQNGTVLHLQKVAGISGSEAHLLALLPRLKERGWNIRFLMLHE
                     HEPGAWEFARELKSRGIPLDSIPLAADVDPFAFLRLAGYLARRRPTILHTHLVHADVY
                     GQLAGALTGVPLRVSTKHGFNEFRENPGFALGDRAIATFAHTHIAISRGLARYLEEVE
                     GFDGSSFEIVHYGIEPDGSPRPYAGAVPVLLCVGRLIPIKGHIVLLRAFAAARRRIPD
                     LQLHIAGRGPLEPALRALAMGLELGDSVRFLGYVAPVQQAIEEAAVVVVPSMGEGFGM
                     VALEAMERARPVIAAEIGGLGELVQDGVTGLLVPPGEAGPLEEAIVRLAGNPSRAAQM
                     GEAGRRRALEHFLQERCTDRTELLYEGALTARS"
     gene            complement(5829..>6075)
                     /locus_tag="VF225_10745"
     CDS             complement(5829..>6075)
                     /locus_tag="VF225_10745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7145773.1"
                     /translation="VYEAAAACLPVLASNPVFDELFDGYPLLFERDSPESLAERLRWF
                     ARLDENERAEIGRTLRERVARQHSVGTWADGILAAAR"
BASE COUNT          944 a         2123 c         2026 g          982 t
ORIGIN      
        1 cgatggaatg ggtcgtcggc acctcgagca gcctgcggtt gtcgcccaac tcgagcaagg
       61 tcggcgtgtc gagctgcagg tgaggtgcgt tcggttccag atatcccggt cgaaaagcgg
      121 tcgcagtgca gtccgcgtag ccgaggtcgg cgaccgcggc ggccactcct tcgtcgaagt
      181 accatccgcc cccgcaaaag aacgtcggtc tgagccctac cgactcgagc cagcttccct
      241 ggtcgcgcac gagcgcagcc gggtcaccgg cggtcggccg cgcatgatcg ggcgcggtcc
      301 agtgtgtgtg cagcccgaag ggcgcccggg cactggccgc ccgtgcccgt tcgagccagc
      361 gttcctcgtc ttctccctcg gccggcgggc gcatcagcgc agcgatcgcg aacccacccg
      421 gcctgcgctc ctggagcgcg ctgaagcgcc tccagacctc gtcgtccaac ggccgctcca
      481 catgacacga gacgaccgcg taccgcccgt gcagtatgtg tcccgtgtgc ggcatcgccg
      541 gactctattc gccagcgggc ccgccgaatc ccgagctcgt tgacgcaatg cgcgcggcac
      601 tcgtccaccg cgggccggac gagggatcga cagacgcttt cggccactgc gtcctcggtc
      661 accagaggct gaaggtcatc gacctcgaga cgggctacca accggtgtcg aacgaggcgg
      721 gcgacgtcat cacgatcttc aacggcgagg cctacaactt cctgtcgctt cgcgacgaac
      781 tgcgcggcca cgacgtgcgc ggcactggtg acactcccat cctgccgcac ctgtacgagg
      841 aaagcggccc gcggtttgtg gaacggctcc acgggatgtt cgcggtcgcg ctctgggacg
      901 ccgggcgcga acgcctcgtg ctcgcacgcg accggctcgg caagaagccg ctcctctgga
      961 cgcggctccc cgacggcacg ctcgccttcg cctcggagct gaaggcgctg ctgcggctgc
     1021 cgcacgtctc ccgggagatc gacctcgaag ccgtcgacga gtacctgtcg ctgcaatacg
     1081 tcccgggcga caggactgcg ctgcgcggca tccacaagct cccgcccgga catgttctcg
     1141 tcgcggaggg cggcagcgaa cggatcgagc cctactggaa gcctgaatca cctgcagagc
     1201 cgagcgcgag cgagcccgaa tggctcgagc gcgtgcgtgc gacggtgggc gaggcggtgc
     1261 gcaaacgtct ggtcgccgac gtgccgctcg gcgctctcct ctcgggcggg atcgattcca
     1321 gcatcgttgt cgcgctgatg gcgacagcgt cgtcccagcc cgtgcgcacg ttcaccgtcg
     1381 ggtttccgga tgcgcgctac gacgagcgag cgtacgcacg cgcggtcgca tcccggtacg
     1441 ccacggtgca cgaggaggtc gagatcgagg aggacgtcgc ctcgacgttg cctcgcctgg
     1501 cagcgacctt cgacgagccg cttggcgacg aggccgcgtt cccgaccttc ctgatcgccg
     1561 agcaggcacg acggcatgtc accgttgcac tggccggcga cggcggggac gagtccttcg
     1621 ccggctacga gcgctacatc gcgcatcgtc tcgccgaccg cttccccgct ccggcggcac
     1681 gggttggctc ggcggcgttg cggcttctcc ccggcgcccg gcgcgagcca cggtccccgc
     1741 tcttccgcgc ggcgcgtttt ctcagcgttg cggccactcc ggccgggcag cggtacgcgc
     1801 gcctgatgga ggtgtttccc ttcgagcttc gccgagagct ctgggccgac gcccggctcg
     1861 cccaacaggt gcggctggag ccgtcgcagc ccggagtcgc cggtctccag ttgctcgaca
     1921 tcgagacgta cctgcccggc gatctgctgc tgaaggcaga cctcgcatcc atggcccact
     1981 cgctcgagct gcgttcgccg tttctcgatc acgaggtcgt cgcgctcggt ctcgctctgc
     2041 ccgaatcact gaagacacgc ggccgggagg gaaaggtggc cctgcggcgt gcgtttgccg
     2101 ccgatcttcc gctcgaggtc gcccgtcgtg gcaaggcggg cttcggcgtt ccgctcggac
     2161 gctggttccg ggccgatctg cgcgacgtcg cgcacgatct cctgtccaat gaccgcgact
     2221 ggttccggcc gggcaccgtg cggcggctgc tcgacgagca cgagtccgga cgcgcggatc
     2281 acgggcaccg cttgtggtgc ctgctgatgc tcgagctctg ggtgcgcgag cacgtggaag
     2341 cgcccgtact cgtcactgcc gcgtgaaacg cggccaactc tggctgatcg ccgttgcggc
     2401 ggtcgctccc cgcctggccg tcttgctgca cgagcgcggt cgcatcctca ctgctttcac
     2461 cgacaagagc gacgacttcg cgcagacgtt cgtgcatcac gggacgttcg gcttcatccc
     2521 cggtgagccg tcggcataca cgcagccgtt gtacggcttc ttcctgatcc cggtgtactg
     2581 gatcttcggg cggacctggt gggccgtcgg cggcgcgcag atcgtggtcg cgacggtgac
     2641 cgcgctcctc gtctatgcga tcggcgcgcg cgtgctgtcg cggcgggcgg gcgcgatcgc
     2701 ggcgatcctc tcgacgctca acccgtacct cgtctggcac gacgtccacc tcaaccggga
     2761 agtgctcgac cagctggttg ctgcggcact catcctctgc acgctcgtcg ccgccgaccg
     2821 gcgctcggtg aagtgggcaa gcctcgcggg aattctgggt ggactcgccg ttctcggcaa
     2881 cgtacgactc gtcttcatcc cgctcgtcgt cgccgcgtac ctggtggtcc gcagcggctg
     2941 gagctgggcg cccgtcgcgc tcgtcgcggt cgctgctttg acagtgacgc cgtgggtgat
     3001 ccgcaacaag gtggaggtcg ggtgcttcgc gctcacgacg gacgggcgcg cactttggaa
     3061 ggcgaacaac gcccagacat acgagctgct cgcgcacggg aagtggatcg acgacgtcaa
     3121 agatccaccc aaccatccgt tccccaaccc ggaggaggca cgagacctct acaagcagta
     3181 cgggaagaag tatcacgtga acgagtgcgc gaaccaggcg tactacgagc acaagacctg
     3241 gaagttcgtg cggaagcatc ccggcgaaaa ggcgaagctc gccgggcagg ccgtgcgcat
     3301 ggagtgggat ccccgcacga cggcttccgc gacagactcg ggccacgggg cgatccgcag
     3361 cctggcacaa ccgctctaca cctcgcttgt gtacgcgctc gcgctgatcg gcatcttcgt
     3421 gaccacgcgc tggtttgtcg ggctcgcgct cgccgtgctc gcctaccaga ccctcgccgc
     3481 ggtcgtcttc gtcggcgcaa ctcgctatcg catcgcgacg gacttcctga tcgcgctcct
     3541 cgcggccggc gcgatcgatt gggcgctgac ccgctggcgg cgccggcagc cgtgaagatc
     3601 ctgcacgtcc accggatcgg aggcattggc ggctccgagc gacatctgct gacgctcttg
     3661 cccgcgttgg ccgcgcacgg agtcgacgtg cgcttcctcg ggctcgacga cacaacgcgt
     3721 gcgcccgatc cgttctacga ggcgctgacc gtgccgtacg tccgcgttcc tgcaccgcgc
     3781 gacatcgacc ctcggctggc gctgcgcgtt cgtcgtgaga cacgaactgc ggacctcgtg
     3841 cacacgcacc tcgtgcatgc ggatgtctat ggagcgctgg gagcgaaacg actcgtctcc
     3901 acgaaacaca atgacgaccc gttccgtgcg ggagcgtttc gcttcgtcga acgcgcgctt
     3961 gcgcggcggg cgtcgaaggt gatcgcgatc accgaagcgc tggcgcggtt ccaggtgcaa
     4021 cgtgtcggcc tgccggccga caaactggag gtcatccact acggcctcga cgagctgccg
     4081 caggcatggg gatcgaatcc gccggatcag gtgcggccgg acgcgcgcgt gctcctcgcc
     4141 gtctgccggc tcgagcctca gaagggcgtg gacgtcgcgg tgcgggcgct gccggacatc
     4201 cgcgcccgcc acccgcaggc cgagctcgtc gtgctcggcg agggcccgca gcgttcggag
     4261 ctcgagcggt tggctcgcga actgcaggtc ccggtgcatc tgcccggccg cgtgccggac
     4321 gtcgcctcgt ggcttcgacg agccgacctg ctcgtccatc ccgcacgctg ggagggcttc
     4381 ggcttggcgc tgctcgaggc gatgttggca gccaagcccg tcgtcgcgac gaacgtcagc
     4441 tcgatcccgg agatcgtggt cgacggccag acgggactgc tcgtcgcacc cgatgatgcg
     4501 gccgctctgg cagccgcagt ggcacgactc ctggacgatc cgggcaacta tggcgagcaa
     4561 ggccgagcgc gcgcgcagtc gcaattcagc gtcgcgcgca tgaccgaccg cacgctcgcg
     4621 ctctacgaga cagcactcca cgagactggt cctcgcaaga ctggtcctgg ggtctgaccc
     4681 caagacatgt cactaaaagg acgtgtcctc gcggcttact agaacgtttc agctgcgggc
     4741 cgtgagggcg ccctcgtaga gcagctccgt gcggtcggtg cagcgctctt gcaggaaatg
     4801 ctcgagcgca cggcggcggc ccgcctcgcc catctgcgct gcccgcgacg gattcccggc
     4861 aaggcgcacg atcgcttcct ccagggggcc cgcttctccg ggaggaacga ggagaccggt
     4921 aaccccgtcc tggacgagct cgcccagacc gccgatctcc gccgcaatca ccggtcgtgc
     4981 gcgctccatc gcctcgagcg cgaccatgcc gaaaccttcg cccatcgacg gcacgacgac
     5041 gaccgcagcc tcctcgatcg cctgttgcac cggggcgacg taacccagga agcggaccga
     5101 gtccccgagc tcgagcccca ttgccagggc gcgcagcgcc ggctccagcg gaccgcgtcc
     5161 ggcgatatgg agttgcaggt ccggaattcg ccggcgagct gccgcgaacg cacgcaacag
     5221 cacgatgtga cctttgatcg ggatcagacg accaacgcag agcaacacag gcacggcgcc
     5281 ggcgtaagga cgaggcgaac cgtcgggctc gatgccgtag tgaacgatct cgaagctcga
     5341 gccgtcgaag ccctcgacct cttcgaggta acgggccaga ccacgtgaga tcgcgatgtg
     5401 cgtgtgggca aaggtcgcaa tcgcacggtc tccgagtgca aagccggggt tctcgcgaaa
     5461 ctcattgaag ccgtgcttgg tcgacacacg caggggcacg cccgtcaacg ctccggcgag
     5521 ctgtccgtaa acgtcagcgt gcaccagatg cgtgtgcagg atcgtcggtc gccggcgcgc
     5581 gagatatccg gcgagacgca gaaacgcaaa cgggtcgacg tccgcggcca gcggaatcga
     5641 gtcgagaggt atgccgcgac tcttcagctc gcgcgcaaac tcccacgccc ccggctcgtg
     5701 ctcgtgcagc atcaggaagc ggatgttcca gccccgctcc ttcagtcgcg gcaggagggc
     5761 gagcaggtgc gcttccgagc ccgagatccc ggccaccttc tgcaggtgca ggacggttcc
     5821 gttctggttc atcgcgccgc cgccaggatt ccgtcggccc aggtcccgac cgagtgctgc
     5881 cgagcgacgc gctcccgcag tgtgcgcccg atctccgcgc gttcgttttc gtcaaggcgg
     5941 gcaaaccagc gcaggcgctc ggccaggctt tccggcgagt cgcgctcgaa gagcagcgga
     6001 tagccgtcga agagctcgtc gaagacggga ttggacgcaa gcaccggcag acaggccgct
     6061 gcggcttcgt acacg
//
LOCUS       DASVEU010000256         9511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1738412,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000256 DASVEU010000000
VERSION     DASVEU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738412"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2870
                     /gene="secD"
                     /locus_tag="VF225_10750"
     CDS             72..2870
                     /gene="secD"
                     /locus_tag="VF225_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7145774.1"
                     /translation="MKDRRNHLIVLGCILAALVGVAFMAAPGSPAYKKPTLGLDLQGG
                     LEVVLKAVPPKNHKLTSDDISRSIDIMRQRIDKLGVSEPEIRKQGTDQIVIQLAGVHD
                     PAAAAKLIGKTAVLEFYDFEADLTGPSVNGLTARVPVASASLFDLLSAPQTRALADKG
                     TPSQWYLFDSKKVVRAGPVPTEQALHTTQLYLKPVAKGGLGKHIPKDWTILKVPQNTV
                     VVSCDAAGGNCLSAQQITSKTGFYLLKHREADKNGQNAIPEMTGGDLKLSGTRADFDT
                     QTSQPVVLMQFTGKGKKIFHTITRREAQRGALICQGRTDATSVQSCVQHFAIVLDGQI
                     QSAPYIDFVRNPDGIPGDNGAQIDMGSSGGIGDAKRLALVLQTGALPVTFETAERTDV
                     SATLGKDSLTQAKKAALVGLLVVALFLLLLYRFLGLVAVVGLGIYAAFLYAAILLFNV
                     TLTLPGFAGLILTIGVAADANVVVFERIKEEVRAGKSVRAAISAGYSKGFHTILDANV
                     VTAITALVLFAVATAQVKGFALMLLIGTVISLITAVGATRAMLGLLAGFSWFNKPRFM
                     GAQGQQTARWLQIDFMRKRYLWFAISGAILVLGIGALGLRGLNLGIDFKGGTQLSFKT
                     PAAHTTGDVRQIMGDQGYNDAVIQGRGASTNSEYKSFQVRTKALSSDKLTSVTRELED
                     KLQATAPGATTVSSSFGRQIAKSAIIAVVFSLFLVVLYIAIRFDLKFAGPVIAAMLHD
                     VLICIGIYALLGREVTSSTVAAILTVLGYSIYDTIIIFDRVRENIPIMRRSSIATIVN
                     VSLWETIRRSLATSFITLLPVGALFLYGGATLKDFAFALMVGIASGAYSSIFIAAPLL
                     TILKEREPEYVRRKGETLSKSGMDAVLEEAEEEAADEPAPELVPVPTLDGPAAAAAAA
                     KRERRRQRRRARPHGRAR"
     gene            2854..3189
                     /locus_tag="VF225_10755"
     CDS             2854..3189
                     /locus_tag="VF225_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145775.1"
                     /translation="MDEPVRRLLLALVGAVALTAERADELADSLAQRGGMSKEEVRAF
                     IDEATTRWRGDAVRVGERAGATLNGAFRELGLVTRDEWDELELRVAQIEHRLRLVESR
                     PRAVPPASS"
     gene            3216..4778
                     /locus_tag="VF225_10760"
     CDS             3216..4778
                     /locus_tag="VF225_10760"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7145776.1"
                     /translation="MGLRRLRGELVLGWAAAAVGATGIASALTPEFANRSDFVRGVLP
                     PGFPGAARILALTFGLTLIWLSRSLARRRRRAWQLAVVLVVGLAIAHLAKGLDVEEAS
                     LSLLVLAALYRFRKRFDVPGDPLFVRPLVVTTLAFATTAALVVVLDVRGFRGDRIEDV
                     LAAFSLVLGFRTLHLWLRPISQRVRQSAEERRLVRRLVRAHGDDSLAFFSLRRDKSYF
                     FSPTRKSFVAYKVVGGCALVSGDPVGDKAELPQLLAEFQRVCHTRGWRLALLSVSSEL
                     LPAARALGLRAIKIGDEAVVRPAAFSLEGRAIRKVRQSVTRLEREGYRFRVVAAGEIE
                     TTLREELESVSAAWRGSNVERGFSMAMDDQFREPETFFAIAERGETVGGFLHVVPSGR
                     GFSLGAMRRRRDTPNGLMEYLIVELLGWARERDAEEISLNFCVFADLIADTPRNKLQS
                     VARATLRVGDRFFQLERLLIFSRKFAPEWRPRYLCVERLSDLPLVGVAYLRVESLLTP
                     PGPWAKRGALKV"
     gene            complement(4944..6071)
                     /locus_tag="VF225_10765"
     CDS             complement(4944..6071)
                     /locus_tag="VF225_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7145777.1"
                     /translation="MLRRERLRSALILAAKLAGAIGVVVLIVIVKPFVRSGQPSTGAN
                     LRPYVSGFHSPTFVGTAPGQPDRLYVVEQAGTIRYVEGRRIAGTLLDIRSRVASGGER
                     GLLSVAFSPTYEKDHLLYVDYTDKHGDTRIVEFHSQDGRADLASARQLLFVRQPYPNH
                     NGGQLQFGPDGLLYVGMGDGGSTGDPGNRAQNRRERLGKLLRIDPSKRGTRWQVVGYG
                     LRNPWRFSFDRATGDLYIGDVGQQAWEEIDFRAHGQLDTLANYGWRVYEGRARYGAGA
                     PAGPGDLVAPVSVYSHEQGCSVTGGYVYRGRSAPAAFGRYFFGDWCTGTIWSLRVDGG
                     RAVDLREEQPHIGQLASFGEGADGTLYAVSVGSGRLFRVVG"
     gene            6108..7775
                     /locus_tag="VF225_10770"
     CDS             6108..7775
                     /locus_tag="VF225_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX7145778.1"
                     /translation="MAQTATRNFGRLSEIAQVAVRHGFGYWFDTHRLTDLFPRRGSRL
                     EVDGQPSQRGQHLREMLDELGPTFVKFGQLLSTRPDVLPPDIIAELRGLQDDVRPFPI
                     EDVEKVIEEDLGLTIAQLFAEFDETPIAAASIGQVHRATLPNGKQVAVKVQRQGAPRQ
                     IEADVALLYQAARIAKERVRALDFIDAQQLVDEFARSIRQELDYRLEARNADTFHRHF
                     AGHPHVRVPKVFWSYTRTRVLTLEWIDGIQVADLDPEHWSLEDRRDIAYLMTETWMTM
                     IFRHGFFHGDPHPANVLVLGSGDQIGLVDFGAVGKLTDDDITKLTRLFIDAAAENVDA
                     LPRRLAELGVRYPKEREEEFLAELREIYYRYYGASLAEIDPLQVIREAFQLIYSMNLR
                     LPSRFLLLDKAIATLGSVGVDLYPDFNVFEVARPYARGLMMERFTPQRMANRARKESL
                     RLGAMALELPYQIHDVLEEFRDGQIEIGFVHKGLDDLLNRLDVVFNRLVIAMIVAGGL
                     IGSSLIGIFAKAGPHVLGINVISIIGFALSGLLGVWLLWGVVRSGRL"
     gene            7845..8474
                     /locus_tag="VF225_10775"
     CDS             7845..8474
                     /locus_tag="VF225_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145779.1"
                     /translation="MSTALLLAEPQADTRGFLERHLTNDGFKVVDAEARPDLVLLGDT
                     AALDACRARHGDVPVIILGEPESDTVDRVRALDRGCDDFLAKPFDYEELLARIRAVLR
                     RTTPAAHETLRAGPIAADLATRCVTVDGVPVALASKEYELLLKLMTDPTRVFTKEHLL
                     REVWDFRSLGRTRTLDSHASRLRRKLSAASPGGVFVVNVWGVGYRLLDA"
     gene            8502..8681
                     /locus_tag="VF225_10780"
     CDS             8502..8681
                     /locus_tag="VF225_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145780.1"
                     /translation="MGQEITDEDRKCPMCGGSKWVLLDGISTLQALEGGLQAIAYSCD
                     ECGFMRWHRSDKVES"
     gene            8678..9043
                     /locus_tag="VF225_10785"
     CDS             8678..9043
                     /locus_tag="VF225_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7145781.1"
                     /translation="MSAFAGLSSLDLQRIWNSVHGRVVHGERITLGVIELDADSHVPE
                     HQHENEQLGMCLSGSLVFRVGDESRELGPGDTWSIPGNVPHEVQVGPEGAVVIDVFVP
                     TRDDWREAERVEQRELRWP"
     gene            complement(9044..>9511)
                     /locus_tag="VF225_10790"
     CDS             complement(9044..>9511)
                     /locus_tag="VF225_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX7145782.1"
                     /translation="LGVLRGADAIVFCHDASAPVDELLAVMDEVTEAEIERPAAIAAT
                     KSDEADGAKLRAAFPELQVVEVSVLDDASVERLRETIWRLTGLMRVYLRDDEHPIALH
                     PPATVLDVADTIHHDLGERCTGARVWGPSSRFDGQQVGRAHVLLDGDTVEILD"
BASE COUNT         1523 a         3198 c         3136 g         1654 t
ORIGIN      
        1 gaggagccgg aagagccgcc tgaggaggcg ccggacgaag agaccccggc tacccttcca
       61 cctgattccg cttgaaagat cgccgtaacc acctgattgt gctcggctgc atcttggcgg
      121 cgctcgtcgg cgtcgccttc atggctgccc cgggctcgcc ggcttacaag aagccgacgc
      181 tcggcctcga cctgcagggt ggcctcgagg tcgtcctgaa ggcagtcccg ccgaagaatc
      241 acaagctgac ctcggacgac atcagccggt cgatcgacat catgcgacag cggatcgaca
      301 agctcggggt ttcggagccc gagatccgca agcagggcac ggaccagatc gtgatccaac
      361 tcgccggtgt gcacgacccg gcagcggccg cgaagctgat cggcaagacc gccgtgctcg
      421 agttctacga cttcgaggcg gatctcacag gcccgtcggt caacggcctg accgcgcggg
      481 tcccggtggc gtctgccagc ctcttcgacc tgctctcggc accgcagacc cgcgcacttg
      541 ccgacaaagg gacgccgtcg cagtggtacc tcttcgactc gaagaaggtg gtgcgggccg
      601 gaccggtgcc gaccgagcag gcgctgcaca cgacgcagct ttacttgaag ccggtcgcca
      661 agggcggcct cggcaagcac atcccgaagg actggacgat cctgaaggtg ccgcagaaca
      721 cggtcgtcgt gagctgcgac gccgccggcg gcaactgcct cagcgcccag cagatcactt
      781 ccaagaccgg cttctacctc ctcaagcacc gggaggccga caagaacggc cagaacgcca
      841 ttcccgagat gacgggtggc gacctcaagc tgtccggcac gcgtgcggac ttcgacacgc
      901 agacgagtca gccggtcgtc ctgatgcagt tcacgggcaa gggcaagaag atcttccaca
      961 cgatcacgcg ccgcgaggcc cagcgcgggg ccctcatctg ccagggacgc actgacgcca
     1021 cctcggttca gagctgcgtg cagcacttcg cgatcgtgct cgacgggcag atccagtcgg
     1081 cgccgtacat cgacttcgtc cgcaacccgg acggcatccc cggtgacaac ggcgcacaga
     1141 tcgacatggg cagcagcggc gggatcggcg atgcgaagcg cctagcgctc gtcctgcaga
     1201 ccggcgcact gccggtcacg ttcgagacgg ccgagcgcac tgacgtgtcg gccacgctcg
     1261 ggaaggactc actgacccag gcgaagaagg cggcgctcgt cggccttctg gtcgtcgcgc
     1321 tcttcctcct gctcctctac cgcttcctcg gtctcgtcgc ggtggtcggg ctcgggatct
     1381 acgcggcctt cctctacgcg gcgatcctgc tcttcaacgt cacgctgacg ctgcctggct
     1441 tcgcgggact gattctcacg attggagttg ccgccgacgc gaacgtcgtc gtcttcgaac
     1501 gcatcaagga agaagtgcgc gccggtaaat ccgtgcgcgc ggcgatctcg gccggctact
     1561 cgaagggctt ccacaccatc ctcgacgcga acgtcgtgac ggcgatcacg gcgctcgttc
     1621 tcttcgccgt cgcgacggcc caggtgaagg gcttcgccct catgctcctg atcggcaccg
     1681 tgatctcgct gatcaccgcg gtcggcgcca cgcgcgcgat gctcggtctg ctcgccggct
     1741 tctcctggtt caacaagcca cgtttcatgg gcgcgcaggg tcagcaaacg gcgaggtggc
     1801 tgcagatcga cttcatgcgc aagcgctatc tctggttcgc catttccggc gcgatcctcg
     1861 tactcggaat cggcgcacta ggcctcaggg gcctcaacct cggtatcgac ttcaagggcg
     1921 gcacgcagct ctcgttcaag acaccggcgg cacacaccac cggcgacgtg cgtcagatca
     1981 tgggcgatca ggggtacaac gacgcggtca tccagggccg cggggcgagc acgaacagtg
     2041 agtacaagag cttccaggtc cgcaccaagg cgctctcgag cgacaagctg acatcggtca
     2101 cccgggagct cgaggacaaa cttcaggcga ctgccccagg agcgactacc gtctcgtcga
     2161 gcttcggccg ccagatcgcg aagtcggcga tcatcgccgt cgtcttctcg ctcttcctgg
     2221 tcgtcctcta catcgcgatc cggttcgacc ttaagttcgc aggccccgtg atcgccgcca
     2281 tgctgcacga cgtcctcatc tgcatcggca tctatgcgct cttgggcagg gaggtgacga
     2341 gctccaccgt tgcggcgatt ctgaccgtcc tcggctattc catctacgac acgatcatca
     2401 tcttcgaccg cgtccgcgag aacattccga tcatgcgccg ctcctcgatc gcgacgatcg
     2461 tgaacgtctc gctctgggag acgattcgcc gctccctggc gacctcgttc atcaccctgc
     2521 tgccggtcgg agccttgttc ctctacgggg gtgcaacgct caaggacttc gcgttcgcgc
     2581 tgatggtcgg cattgcttca ggcgcctatt cctcgatctt catcgccgca ccgctgctca
     2641 cgatcctcaa ggagcgcgag ccggaatacg tgcggcgcaa gggcgagacg ctcagcaaga
     2701 gcggcatgga cgcggtcctc gaagaggcgg aggaggaggc cgccgacgag cccgcgccgg
     2761 agctcgttcc cgtcccgacc ctggatggac ccgcggctgc cgccgcagct gcaaaacgcg
     2821 aacgccggcg tcagcgcagg cgtgcgcgcc cgcatggacg agcccgttag acgtctcttg
     2881 ctcgcgctgg tcggtgccgt ggcgctgacg gcggaacgcg cggacgagct cgccgattca
     2941 ctcgcgcagc gcggtgggat gagcaaggag gaggtgcgcg ctttcatcga tgaagcgacg
     3001 acgcggtggc gcggtgacgc cgttcgggtc ggcgagcgcg ccggcgcgac actcaacggc
     3061 gcgtttcggg agctcggtct cgtgacccgc gacgaatggg acgagctcga gctccgcgtc
     3121 gcccagatcg agcacagact gcggctcgtg gagtcacggc cacgtgccgt gcctccagca
     3181 agcagttagc cggatcccca gctacccttc caggcgtggg gcttcgccga cttcgcgggg
     3241 agctcgtgct cggttgggcc gccgcagcgg tgggagccac cggcatcgcc tctgcgctca
     3301 ctcctgagtt cgccaaccgc tcggacttcg tgcgcggcgt gctcccgcct ggtttccccg
     3361 gcgccgcgcg catcctggcc ctcaccttcg gactgacgct catctggctc tcgcgctcgt
     3421 tggcgcgcag gcgtcgtcgc gcgtggcagc ttgcggtggt gctggtcgtc gggttggcga
     3481 ttgcgcatct cgcaaaaggt ctcgacgtcg aagaagcgtc gctgagcctg ctcgtgctcg
     3541 cggcgttgta tcggttccgc aagcgcttcg acgtgcccgg cgatccactt ttcgtgcggc
     3601 ccctggtcgt caccaccctc gcgttcgcga cgaccgccgc gttggtcgtg gtgctcgacg
     3661 tgaggggatt ccgcggcgac cggatcgaag acgtcctcgc ggctttcagc ctcgtgctcg
     3721 gcttccggac gctgcacctc tggctacggc cgatctcgca acgggttcgt cagtccgccg
     3781 aggagagacg cctcgtccgc aggcttgtcc gcgctcacgg cgacgacagc ctcgccttct
     3841 tcagcctccg tcgggacaag agctacttct tctcgccgac acggaagagc ttcgtcgcgt
     3901 acaaggtcgt cggcggctgt gcgctcgtga gcggcgatcc cgtcggcgac aaggccgagc
     3961 taccgcagtt gctcgcggag ttccagcgcg tgtgtcacac acgcggctgg cggctcgcgc
     4021 ttctcagcgt cagcagcgag ctgctgccgg cggcgcgcgc gctggggctg cgcgcgatca
     4081 agatcggcga cgaggcggtc gtgcgaccgg ccgcattctc gctcgagggc cgcgcgattc
     4141 gaaaggtccg acagtcggtc acgcgcctgg aacgtgaggg ctatcggttc cgcgtcgtcg
     4201 ccgccggtga gatcgagacg acgctgcgcg aggagctgga gagcgtgtcc gccgcctggc
     4261 gcggctcgaa cgtcgagcgc ggcttctcga tggcgatgga cgaccagttc cgcgagcccg
     4321 agaccttctt tgccatcgct gagcggggtg aaacggtcgg cggtttcctc catgtcgtgc
     4381 cgagtgggcg tggtttctcg cttggtgcga tgcgccggcg ccgcgacacg cccaacggcc
     4441 tgatggaata cctgatcgtc gaattgctcg gctgggcgcg tgagcgcgac gcggaggaga
     4501 tctcgctcaa cttctgcgtg ttcgccgatc tgatcgccga cacgcctcgc aacaagcttc
     4561 agtccgtcgc gcgggcaacg ttgcgcgttg gcgatcggtt cttccagttg gaacggctcc
     4621 tgatcttcag ccgcaagttt gcgcccgaat ggcgtccgcg ctacctctgc gttgagcggc
     4681 tttccgatct gccgctcgtc ggagtggcgt atctgcgggt cgagtcgctc ctgacaccac
     4741 cgggcccgtg ggcgaagcgc ggcgcgctca aggtttagag gcctgatgtg aaattccgcc
     4801 gggccggatg acgcaccaga cacggcgccg gatcagcacg ctcgcccgcg gcacgtagct
     4861 ccgctacgca ccgcgaacga ccgcactgcc cgacttggtc tggagcgccc cccggcgacg
     4921 gccgaattcc acaccagacc tctctaacca accactcgga agaggcggcc gcttccgacc
     4981 gacacggcgt agagcgtccc gtctgcgcct tcaccgaagc tcgctagctg cccgatgtgg
     5041 ggctgttctt cgcgcaggtc aacggcgcga ccgccgtcga cgcgcaggct ccagatcgtg
     5101 ccggtgcacc agtcgccgaa gaaatagcgt ccaaaggcgg cgggtgcgct ccggccgcgg
     5161 tagacatagc cgcccgtcac cgagcagccc tgctcgtgcg agtagacgct gaccggcgct
     5221 accaggtcgc cgggacccgc cggagctccg gcgccgtaac gtgcgcgccc ttcgtagacc
     5281 cgccagccgt agttcgcgag ggtgtcgagt tggccgtgcg cgcggaaatc gatctcttcc
     5341 caggcttgct ggcccacgtc tcctatgtac aggtcaccgg ttgcgcgatc gaaggagaag
     5401 cgccagggat tgcgcaggcc gtagccgacg acctgccagc gtgtgcctcg tttggacgga
     5461 tcgatccgaa gcagcttgcc cagccgctcg cggcggttct gcgcccggtt gccgggatcg
     5521 ccggttgagc cgccgtcgcc catgccgacg tagagcagcc cgtcagggcc gaattgcagc
     5581 tgaccgccgt tgtgattcgg atacggctgg cgcacgaaaa ggagctgccg tgcgctggcg
     5641 aggtccgccc tgccgtcttg cgagtggaac tcgacgatgc gcgtatcacc gtgcttgtct
     5701 gtgtagtcga cgtagagcag atggtccttc tcataggtcg ggctgaacgc aaccgacagc
     5761 aggccgcgct cgccgccgct tgcgactctc gagcggatgt cgagcagggt tcccgcgatg
     5821 cgccgtcctt cgacgtagcg gatcgttccg gcctgctcga cgacgtaaag acgatcgggc
     5881 tgcccaggcg ccgttccgac gaacgtgggg ctgtggaaac cggagacgta cggccggaga
     5941 ttggcgccgg tcgacggctg cccggaacgc acgaagggct tcacgatcac gatgagcacg
     6001 acgacgccga tcgcgccggc cagcttcgca gcgaggatca gggctgacct gagccgctcc
     6061 cgccgcaaca cacgaccagc gtacagttcc gtgcaacact tgaagcagtg gcgcaaacgg
     6121 caacccgcaa cttcggacgt ttgtcggaga tcgctcaggt cgcggtccgg cacggcttcg
     6181 gctactggtt cgacactcat cggctgaccg acctcttccc gcggcgtggg agtcggctcg
     6241 aggtcgacgg tcagccttcc cagcgcggcc agcatctgcg ggagatgctc gacgagctcg
     6301 gcccgacgtt cgtcaagttc ggccagctgc tctcgacgcg gcctgacgtg ctcccgcccg
     6361 acatcatcgc cgagctgcgc ggcctgcagg acgacgtccg cccgtttccg atcgaggacg
     6421 tcgagaaggt gatcgaggag gatctcggcc tcacgatcgc ccagctcttc gcggagttcg
     6481 acgagacgcc gatcgccgcg gcgtcgatcg gacaggtgca ccgcgcgacg ctgccgaacg
     6541 gcaagcaggt tgccgtcaag gttcagcgcc aaggcgcgcc gcgacagatc gaagcggacg
     6601 tggcgcttct ctatcaggcg gctcgcatcg ccaaggaacg tgtgcgtgcg ctcgacttca
     6661 tcgacgcgca gcagctcgtg gacgagttcg cgcgctcgat ccgccaggag ctcgattacc
     6721 gcctggaggc acgaaacgcg gacacattcc accggcactt cgccggccat ccgcacgttc
     6781 gcgtgccgaa ggtcttctgg agctatacgc gcacccgcgt gctgactctc gagtggatcg
     6841 acggcatcca ggtcgccgat ctggatccgg aacattggtc gctcgaagac cggcgcgaca
     6901 tcgcgtacct gatgacggag acgtggatga cgatgatctt ccgccacggg ttcttccacg
     6961 gcgatccgca tccggccaac gtgctcgtgc tcggcagcgg cgatcagatc ggtctcgtcg
     7021 acttcggcgc cgtcgggaag ctgacggacg acgacatcac gaagctgacc cggctgttca
     7081 tcgatgccgc cgcggagaac gtcgacgcgc tgccccgccg cctggccgag ctcggcgtgc
     7141 gctacccgaa ggagcgggag gaggagtttc tcgccgagct gcgcgagatc tactacaggt
     7201 actacggggc cagtctcgcc gagatcgacc cgctgcaggt catccgcgag gccttccagc
     7261 tgatctactc gatgaacctc cgtctgccgt cgcggttcct cctgctggac aaggcgattg
     7321 cgacgctcgg ttccgtgggc gtcgatctct atccggactt caacgtcttc gaggtggcgc
     7381 ggccgtacgc acgcgggctg atgatggagc ggttcacgcc gcagcgaatg gcgaaccggg
     7441 cgcggaagga atcgttgcgc ctgggggcga tggcgctcga gcttccgtac cagatccatg
     7501 acgtgctcga ggagttccgg gacgggcaga tcgagatcgg cttcgtgcac aaggggctcg
     7561 acgatctgct caatcggttg gacgtcgtct tcaaccggct cgtgatcgcg atgatcgtcg
     7621 ccggcgggct gatcggctcg agcctgatcg ggatctttgc caaggcgggc ccgcacgtgc
     7681 tcgggatcaa cgtcatctcg atcatcgggt tcgcgctctc cggcctgctc ggagtgtggc
     7741 tcctttgggg tgtcgtccga agtggccgtt tgtagatcgt tcgtcacgct ttcgcgagaa
     7801 aggacatctg gtctcaccct cgcgccggcg ctacgttcgc tcacatgtcg acggcgctgc
     7861 ttcttgccga accacaagcc gacacgcgcg ggtttctcga gcggcatctg accaacgacg
     7921 gcttcaaggt cgtcgatgcc gaggcgcgac cggatctcgt cctcctcgga gacacggctg
     7981 cgctggacgc ctgccgcgcg cgtcacggcg acgtgccggt catcatcctc ggcgaaccgg
     8041 agtcggacac cgtcgatcgc gtccgcgcac tcgaccgtgg ctgcgacgac ttcctggcga
     8101 aaccgttcga ctacgaggag ctactggccc gcatcagggc cgtgttgcgg cggacgacgc
     8161 cggcggccca cgagaccttg cgcgcggggc cgatcgccgc cgacctcgcg acgaggtgcg
     8221 tgacggtcga cggcgtgccg gtcgccctcg caagcaagga gtacgagttg ctgctcaagc
     8281 tgatgacgga tcccactcgt gtgttcacaa aggagcacct gctgcgggag gtctgggact
     8341 tccgctcgtt gggacgcacg agaacgctgg actcgcacgc ctctcgcctt cgccggaagc
     8401 tgtcggccgc ctcgcccggc ggcgtgttcg ttgtcaacgt gtggggtgtc ggttaccggc
     8461 tgctggatgc gtagttcggc gcgggcgcta ggctcaccga cgtgggtcag gagatcacgg
     8521 acgaagaccg gaagtgcccg atgtgcggcg gctcgaagtg ggtcctcctc gacggcatct
     8581 cgacactcca ggccctggag ggagggctgc aggcgatcgc ctactcctgc gacgagtgcg
     8641 gcttcatgcg ctggcaccgt tctgacaagg ttgagtcgtg agcgcgttcg ccggtctctc
     8701 ctcgctcgac ctacaacgga tctggaacag cgtgcacggt cgcgtcgttc acggcgagag
     8761 gatcaccctc ggcgtgatcg agctcgacgc cgacagccat gttccggagc accagcacga
     8821 gaacgagcag ctcgggatgt gtctcagtgg ctcgctcgtg tttcgcgtcg gtgacgagag
     8881 ccgcgagctc ggtccgggcg acacgtggtc gattcccggc aacgtcccgc acgaggtcca
     8941 agtcgggccg gaaggtgcgg tcgtcatcga cgtattcgtg ccgacgcgtg acgactggcg
     9001 ggaggccgag cgtgtcgagc agcgcgagct gcgctggcct tagttagtcc aggatctcga
     9061 ccgtgtcacc gtccagcagc acgtgggcgc ggccaacctg ctgaccgtcg aagcgcgacg
     9121 agggacccca gactcgggct cccgtgcacc gctcgccgag gtcgtggtgg atagtgtcgg
     9181 cgacgtccaa cacggtggcg ggcggatgca gtgcaatcgg atgctcgtcg tcccgtagat
     9241 agacgcgcat cagtccggtc agccgccaga tcgtctcgcg caggcgctcg acgctcgcgt
     9301 cgtcgagcac gctgacctcg accacctgaa gctccgggaa cgccgcgcgc agcttcgccc
     9361 cgtcggcctc gtcgctcttc gtcgccgcga tcgccgccgg gcgttcgatc tcagcttccg
     9421 tcacctcgtc catgaccgcc agcagctcgt cgaccggagc gcttgcgtcg tggcagaaca
     9481 cgatggcgtc tgcgccgcgg agcacgccga g
//
LOCUS       DASVEU010000257         5478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1156253,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000257 DASVEU010000000
VERSION     DASVEU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156253"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1158
                     /locus_tag="VF225_10795"
     CDS             151..1158
                     /locus_tag="VF225_10795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7145783.1"
                     /translation="MSDLGGGLDALASRTGFSGVVRIDQSERIELARAYGLAHRGFEI
                     PNAVDTRFGLASGAKGFTALAVVTLIEEGVLEFSTPARSVLGSDLPMIDEGVTVEHLL
                     SHRSGIGDYLDEEAGFEVEEYVMPVPVHELATTEDYLAVLDGHASKFAPGERFAYCNA
                     GYVVLALIAERASGMPFHELVEQRVCRRAGLTDTAFLRSDELPARTALGYVEVDGAWR
                     SNVFHLPVRGSGDGGIYSTAADMSSFWRAFFAGRIVSTDWVSEMVRPRSDAPKNSKRY
                     GLGFWLHASNDAVLLEGNDAGVSFRSVHDPRARVTLTVISNTSDGAWPIARYLENAAS
                     S"
     gene            1301..1915
                     /locus_tag="VF225_10800"
     CDS             1301..1915
                     /locus_tag="VF225_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7145784.1"
                     /translation="MAKRSAQISGGASPAEGASYETRPLDASTWDAFAELVERNNGVY
                     GGCWCIAHHSEYQRGVSDPRTLKEELVRAGRAHAALVFDEDDLAQGWCQYGSPEELGL
                     KHQREYLKNPPPRARWRIACIFVDKGHRGRGVARAGLEGALAQIAARGGGLVEAISEV
                     TAGREAQGRFLFSATAELFEQYGFARVRQVGKHAWIVSREIDPA"
     gene            2721..3197
                     /locus_tag="VF225_10805"
     CDS             2721..3197
                     /locus_tag="VF225_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7145785.1"
                     /translation="MGPLDSDLEDLYRRRQGAFQVMLASVTGSVESARDVVQEAFARA
                     LQNQNGFRGDGSLEGWVWRIAFRVAVGSQGSRELSLDEVPEVAFVDETNDPALASAVQ
                     TLPRQRRMAIFLRYFADLSYSEIGDVLGIAEGTVAATLSKAHEQLGAELSINEVTA"
     gene            3194..4327
                     /locus_tag="VF225_10810"
     CDS             3194..4327
                     /locus_tag="VF225_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145786.1"
                     /translation="MSTYDESLLAARFAALAPEPLIGDWDDVLDRARTARNGRGLLGR
                     LRPSAFRGRRRRLVLMLAAAALVVVVATASALAVRAFILDRGFIGLPPQGATPSAPES
                     GELVVLWLGRSATNGALVRSWVYADGRIIWSRSGSIPEGANELTSGYLEQRLTPEGVE
                     LLRSAVAGLFDGSRTLLETVPAPYPGVGLVGSSPLLVPPGPADWGLVEVPRGDRLVRL
                     AWLPAGSGEQTATPEQLSALRRVDALLTDPASVLPPSAWAIREVKAYVPSHYAVCIET
                     SPPKAASQLLSLLPARAADVLRANSVTRLDWGVAATTYCSKLATEKAREVAEALSGLD
                     QDPRWQRHTLAYRVAEGAHNWEATAIWFEPYFPHGQFTYSASG"
     gene            4619..5299
                     /locus_tag="VF225_10815"
     CDS             4619..5299
                     /locus_tag="VF225_10815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7145787.1"
                     /translation="MSDLGRGSTRLRPGQVLGCRAVVFDFGYTLVNEDRVWGAIAGQY
                     GWSPSVFFSTLGAVIERRGRHRDVFELLGAAQPYEPVQFEPGDFYDDALPAIRAVKRK
                     GWVVGIAGNMRTEIERFLSRHVDVDFIASSERWKVEKPDNEFFAKIAVAAEASPEDVV
                     YIGDRIDNDIVPAARAGMIAVHLRRGPWAAIQQSWPEARAAATTITELNAIPGMGELR
                     SRWLSRSD"
BASE COUNT          856 a         1779 c         1927 g          916 t
ORIGIN      
        1 gccaggttcg ggcgtgtcgc aagcgcacgt cccaacacac gccccgtcgc gtgggccagc
       61 gagcgggaat gtcccagaac ggggaagagc gcggctgggt gagcccgggc actcggcggc
      121 cggatgcacg caccggctag cgtctgccac atgtctgatc tcggaggcgg gctcgacgcg
      181 cttgcgtccc ggacagggtt ctcgggtgtc gttcggattg accaaagcga gcggatcgag
      241 ctcgcgaggg cgtacggcct tgcgcatcgc ggcttcgaga tcccgaacgc ggtggacacg
      301 cgcttcggac tcgcgagcgg agcgaaaggc ttcacggcgc tcgccgtcgt gaccctgatc
      361 gaggaaggcg tgctcgagtt ctcgacgccc gcccgctccg tcctcggcag cgatctgccg
      421 atgatcgacg agggcgtgac ggtcgagcac ctcctgtcgc accggtcggg catcggcgac
      481 tacctcgacg aggaagccgg attcgaggtc gaggagtacg tgatgccggt accagtgcac
      541 gagctcgcga ccaccgagga ctacctcgcg gtgctcgacg gccacgcgag caagttcgcg
      601 cccggcgagc gcttcgcgta ctgcaacgcc ggctacgtcg tgctggcgct cattgccgaa
      661 cgcgcgagcg gcatgccctt ccacgagctg gtcgagcagc gcgtgtgccg gcgcgccgga
      721 ttgacggaca cggcgttcct gcgctccgac gagctccccg cccggaccgc cctcggttac
      781 gtcgaggtcg acggcgcgtg gaggagcaac gtcttccatc tccccgtgcg ggggagcggc
      841 gacggcggga tctactccac ggccgcggac atgagctcgt tctggcgggc gttcttcgcc
      901 ggtcggatcg tctccacgga ctgggtttcg gagatggttc ggccgcgcag cgacgcgccg
      961 aaaaactcga agcgctacgg gctgggcttc tggctccacg cgtcgaacga cgccgtcctc
     1021 ctggagggga acgacgcggg tgtctctttc cggtccgtgc acgatccgcg cgcacgcgtg
     1081 acgctcacgg tgatctcgaa cacgagcgat ggcgcgtggc cgatcgctcg gtatctcgag
     1141 aatgcggctt caagctgagc gactttcctc gtaagtcggc ccacgcgggc agtgggtggg
     1201 tagtgaaggt cgcctgcgcc agcgttcggg aatgtcaccc gagcactctc gttccgcctg
     1261 ctctcgacga cccggaataa gctctcgact gaagccgccg atggccaagc gatcagcaca
     1321 gatatccgga ggggcgagcc cggccgaggg ggcttcctac gagacccgcc cgctcgacgc
     1381 gtcgacgtgg gacgcctttg cggagctcgt cgagcgcaac aacggggtct acggagggtg
     1441 ctggtgcatc gcccatcact ccgagtacca gcgcggcgtc agcgatcccc gcactctgaa
     1501 ggaggagctg gtcagggcgg gccgtgctca tgccgcactc gtcttcgacg aggacgacct
     1561 cgcacagggc tggtgccagt acggcagtcc ggaggaactc gggctcaagc accagcgtga
     1621 gtacctgaag aacccgccgc ctcgtgcgcg ctggcggatc gcctgcatct tcgtcgacaa
     1681 aggccatcgc ggccgcggag tcgcgcgggc cgggctggag ggcgcgctcg cccagatcgc
     1741 cgcccgcggc ggcggtctcg tcgaagcgat ctccgaggta accgccggac gggaggctca
     1801 aggccggttc ctgttcagcg cgaccgccga gctgttcgag cagtacgggt tcgcgagggt
     1861 acggcaggtc ggcaagcacg cctggatcgt gagccgcgag atcgaccccg cgtaggggtc
     1921 acgcacaagc cgcaggaaga gacactgcag aaagagaccc cgctgcgcag gcggagaccg
     1981 tcaagccctg catccactcg ctattcctgg acaggtagcc gtgacctggg ccagcgttcg
     2041 ggaatgtcac ctgagcgcct ggcaaccttc ttcgtcgtgc cgatcgtccg aaggtactcc
     2101 cgagagcaag cgctcgacgt cgctcgtgcc gaggcaaacc gtcgaggttg ggactggcgc
     2161 gagccagtcg tgctggagca cctactttcg taccgcatct ttccgagtgg ctacgccggc
     2221 ggcccctgga cagggccgat gatcgttgtg tcggcatggt cgggccgtgt gaaacaggct
     2281 ggcatcgcgc ctcgatgacg gtcggctccg tcggttgggc cactgttcgc gaatgtcctc
     2341 tggcccacgg gccgcctcga actcgtcgaa gatgcgtaca tgaccgaagg taactgcgtc
     2401 ttctgtgagc gttaccggtg tgggtggaag gccggtcgag ctcggcgggc tgctgctgcc
     2461 agctgaactt cgcagattcc ctctacccgg ggtctgcacc gcgcttgcag gccggacaac
     2521 gtttgaccgt taccggtgtg ggcggaaggc cggtcgaggt cggcgggctg ctgctgccag
     2581 cgcgaggtgt tcaagcaatg tctcgggacg cccggcctgg gatgcggtgg gacgcccggt
     2641 gcgacccacg gtcatgatcg cttcacgatg gatacatcgg gcccagtaaa ggtttcgcct
     2701 gccatctagc tcttatgagc atgggtcccc ttgactccga cctggaagac ctctaccggc
     2761 gacggcaggg cgccttccag gtgatgctcg catcggtaac gggcagtgtc gagagcgcac
     2821 gcgatgtcgt gcaagaggcg ttcgcacgcg cgctgcagaa ccagaacgga ttccgcggtg
     2881 acgggtcgct cgaggggtgg gtgtggcgaa tcgcgtttcg cgtcgcggtc ggctcgcagg
     2941 ggtcgcgcga actctcgctg gacgaggtgc cggaggtggc tttcgtggac gagacgaacg
     3001 atccggcgct tgcgtccgca gtacagacac tgccgcggca gcgacggatg gcgatcttcc
     3061 tgcggtactt cgcggatttg tcctattccg agatcggcga cgtgctcgga atcgccgagg
     3121 gaacggtcgc ggcgacgctg tcgaaggcgc acgagcagct cggcgccgag ctttcgatca
     3181 atgaggtgac ggcatgagca cgtacgacga gagtctgctg gccgcgcggt tcgccgcgct
     3241 ggcgcccgag ccgctcatcg gggactggga cgacgttctc gatagagccc gcacggctcg
     3301 gaacggccgc ggtctgcttg ggcgcctgcg ccccagcgcg ttccgcggtc ggcgtcgccg
     3361 gctcgtgctc atgctcgccg cggcggccct cgtcgtcgtt gtggcaaccg cctctgcgct
     3421 cgcggtgcgc gccttcatcc tcgacagggg cttcatcggt ttgccgccac agggagcgac
     3481 gcccagcgcc ccggaaagcg gcgaactcgt cgtcctttgg ttgggcagga gcgcgaccaa
     3541 cggagccctc gtccgctcct gggtgtacgc cgacgggcga atcatctggt ctcggtcggg
     3601 ctccatcccc gagggcgcga acgagctcac ctccggctat ctcgagcaac gactcacgcc
     3661 ggagggcgtg gagctcctgc ggtcggcggt cgccggactc ttcgacggca gtcgcactct
     3721 cctcgagact gtcccggccc catatccggg agtgggcttg gtcggcagct ccccactcct
     3781 cgttccgcca ggtccggccg actgggggtt agtggaggta ccccgcggcg accggctcgt
     3841 ccgcctcgcg tggttacccg ctgggtccgg ggagcagacg gcgacgccgg agcagctttc
     3901 ggctctccgg cgggtcgacg cgctgctcac tgacccggcg tcggtgctgc cgccgagcgc
     3961 gtgggccatt cgtgaggtca aggcctacgt gccatcgcac tacgcagtgt gcatcgaaac
     4021 ctcgccgccg aaggctgcat ctcagctcct gtccttgctg ccggcgcggg ccgcggacgt
     4081 gctccgcgcc aacagcgtga cacggctcga ctggggagtg gccgctacca cgtactgctc
     4141 caagctggcg accgagaagg ctcgcgaggt cgccgaggct ctctccgggc tcgatcagga
     4201 cccgcgatgg cagcgtcaca cgcttgcgta tcgcgtggct gaaggggccc acaactggga
     4261 ggcgacggcg atctggttcg agccgtactt ccctcacggc cagttcacct actcggcaag
     4321 cggatgagca tccgggcttg gcgagcggag cgctacagaa cggaggaacc atgcatgctc
     4381 ggcacatcgt ccccgccatg ctcgctgccg aacgtgtcgc agaacccgct cgggtacgac
     4441 gcctcgaacg tttcgtctcc cgggcggagc ggatagggcg caacctcagg ttccccggag
     4501 tggttcacct agggcgaggt ctacgggctg ggccagcgtg cgggaatgaa gggagagcgc
     4561 ggctgggtga gccggggcac tcggcggccg gatgagcgca ccggctagcg tctgccgcat
     4621 gtctgatctc ggacgcggct cgacgcgctt gcgtccggga caggttctcg ggtgtcgtgc
     4681 cgtcgtcttc gacttcggct acacgctcgt gaacgaggac cgggtctggg gcgccattgc
     4741 cgggcagtac ggttggtcgc cgtcggtttt cttctcgacg ctcggagctg tcatcgagcg
     4801 ccgggggcgc caccgcgacg tcttcgagct gctcggcgcg gcgcaaccct atgagccggt
     4861 tcagttcgag cctggggact tctacgacga cgcgctcccc gccatccggg ccgtcaagcg
     4921 gaaggggtgg gtcgtcggga tcgccggcaa catgcgcacg gagatcgagc ggttcctgtc
     4981 tcggcacgtc gacgtcgact tcatcgcctc gtcggagcgg tggaaggttg aaaaaccaga
     5041 caatgagttc ttcgcgaaga ttgccgtcgc tgcggaggcc tcgccggagg acgtcgtcta
     5101 catcggagac cggatcgaca acgacatcgt gcccgccgcg cgggcgggaa tgatcgccgt
     5161 gcacctccgg cgcggcccct gggccgcaat ccagcaaagc tggcccgagg cgcgggccgc
     5221 cgctacgacg atcacggagc tcaacgcgat tcccggcatg ggcgagctgc gtagtcgctg
     5281 gctgtcaagg tccgattgaa cagtcgctcg atggtgcgat ttgcccatac cgcagtaagt
     5341 agggggtagg gccagaacgc cggcctgggc gagcgtactg tcgcctgagg caacttgact
     5401 acggagagca ccgcaacgcg cccagcgggt ggcgcgtaac aagtggagcg tgccgggatc
     5461 gaaccggcga cctccggc
//
LOCUS       DASVEU010000258         8562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_770655,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000258 DASVEU010000000
VERSION     DASVEU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770655"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VF225_10820"
     CDS             <1..739
                     /locus_tag="VF225_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7145788.1"
                     /translation="FKVVGLVRPPLGGQTADVYIPLAQLQKLAGQKGLANVVLVRATS
                     SSDVAKVQQEIEQALGSGAQVASAKQVAASINGSLVDAANLSHNLGLVLAIIAAVAAF
                     LVAALLTLSSIGKRVREIGTLKALGWSQRLVVRQVLGECLATGVAGGLVGVVLGVLAA
                     ATIGAFGPSLTASSTTGAAGANDVLGLGQALARTASTSVSLTAPLSLSLLLFGFALAL
                     AGGLIAGGAGALRAARLRPADALRQME"
     gene            736..1428
                     /locus_tag="VF225_10825"
     CDS             736..1428
                     /locus_tag="VF225_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7145789.1"
                     /translation="MTGDLLYDVRNATRSFRRGEATINALSGIDLTIEHGEIVALVGP
                     SGSGKTTLLQLLGALDRPTSGQIFFEGRDLNALADNELADLRLRAFGFVFQTFNLIPT
                     LNALQNVEAKLAPTGVPEDELRARSLALLEEVGLSDRADHLPSQLSGGEQQRVAIARA
                     LSTEPRVVLADEPTGNLDSRTGTEVVDLLTSLSEERRHTVILVTHDAEIAEKAHRSIR
                     MQDGRLLEPTPA"
     gene            1458..2030
                     /locus_tag="VF225_10830"
     CDS             1458..2030
                     /locus_tag="VF225_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX7145790.1"
                     /translation="MTHALIVTLAALGVVGLVLAGLLLLTGLLWLSGHRLPLRTFRRW
                     LWGYELWLAFLVAAVATGGSLFFSEVAHFVPCELCWFQRICMYPLSITTLLMALADEH
                     RAARYLLPLPLVGAGVSTYHLLVENGVVKQAQACLLSAPGGCATKWINEFGFVTIPTL
                     ALTAFVLVFAALLFSAIEPVVPTAEAKAET"
     gene            2055..2861
                     /locus_tag="VF225_10835"
     CDS             2055..2861
                     /locus_tag="VF225_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7145791.1"
                     /translation="MPSGKQSKRQRGAARTGPPPVQRKGRRRQVSRRALLTAAAVIVL
                     AAIGLTLGVVLSAGSSKASTPARGSLVNALPGAADVQRLLSGIPQSGNVLGSRSAPVT
                     VVEYVDLQCPYCQQFETQAMPGLIGQYVRTGKVKIEARPIAFIGPDSQRGRAAAIAAG
                     EQNKLFNFAQVLYLNQGSENTGWLNDQMIESAASSIPGLDVPQLLSTRSSATVNTRAD
                     AFDQQAVADNVQATPTILVGKTGGTLRPVALKSPTDGQSVASAINAALRS"
     gene            2992..3567
                     /locus_tag="VF225_10840"
     CDS             2992..3567
                     /locus_tag="VF225_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145792.1"
                     /translation="MIEDIRPDLARWVTPHPEWKPEEDYLDESYREVASALHVTPDAL
                     VLVDPLVPDSLWPELDARIEASGKPVVVLTTIHWHARSRDAVAKRYDARLGGEPQGVR
                     AFDAGRQDEVALWLEGARAVVLADAVVGDQHGGLRLSPWFKFEAEQERTRLALLPLLD
                     LPIAIVLPAHGNAVLEDGKQALEKALARAYT"
     gene            3731..6829
                     /locus_tag="VF225_10845"
     CDS             3731..6829
                     /locus_tag="VF225_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7145793.1"
                     /translation="MPHCPNCGHYSAKAFEFCARCGSPTATVSSREQRKRVTVFFCDV
                     IASTALGEGLDPESLRRMLTRYFETAQQVIEAHGGTVEKFIGDAVMAVFGVPVLHEDD
                     ALRAVRAARVLRDAMVSLNEELEREYGNRLVLRIGVNTGEVVTGTEERLATGAPVNLA
                     ARLEQAAAPGEILLGAETLALVRHAVVAEPLAPLAVKGKREPVSAWRLLAVRGETGFA
                     RRFDVPMLGRETELRRLREAFEQTQRDRSCQLVTILGAAGVGKSRLAHEFLAALDGVA
                     VLRGRCLSYGEGITYWPVVEIVRQLEPRLAQLPLDGEVLATLRGFLGPDDAMDSIEEI
                     AFAVRRLLEAAARELPLVCVFDDIQWGEPAFLELIEQVAVLSRDAGLLLCCLARPELL
                     ERHPAWGGGMLNSATVVVEPLSGDETDQLIEHLATDSLRTGALKGRIRQAAEGNPLFV
                     EEMIALLRDSPERDVSVPPTIHALLTARLDQLDPDERALLQRAAVEGRVFHRGAVQAL
                     APEETQIGSRLAALVRKELLGPDRPQLKRDDAYRFRHLLIRDVAYDSLPKATRAELHE
                     RLADWLGKRASELVEADEILGYHLEQAVRYRLELRPLDDYGRELGIRAAGLLAGAGAR
                     ALGRNDVGAALKLLRRAVALRPPDDPAVALRLDLSQALLFSGQFAEAEEVTQEAAARA
                     VETGDQCGAMRARLMTLRISTQTPSQEGSETSPSDELLALAEEALPLFEHVGDEAALT
                     EAWLAIAWAQLIRCGWGEMLAAVDHALDHARRAGYARWERELPAWKSTALFHGPTPVD
                     EVLDWHREQQSQHPIALNDQAVLEAMRGAFDRARALAAAGHASAEALGQTFSLAAEGM
                     IGWEVETLAGDPSAAELNARRSCELLEQLGDTAVRSLASGQLAESLYALGRLDEARQW
                     TDVAEELSTNDDVVSQMVWRQVRAKVLARDGKHAEAESLAREAIRLAERTDMVNRQGH
                     ARADLAEVLTEAGRKEQAAAELDVALAYYERKGNLVAAANARRRLAELRGRAVVKPRP
                     GG"
     gene            6885..7094
                     /locus_tag="VF225_10850"
     CDS             6885..7094
                     /locus_tag="VF225_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145794.1"
                     /translation="MESGYENDNLERLEKEGMFRKSPLPEKYQEVINDLPRESVDALI
                     SVKQRFDRAGLSPEESEFREYIHWF"
     gene            7265..7780
                     /locus_tag="VF225_10855"
     CDS             7265..7780
                     /locus_tag="VF225_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX7145795.1"
                     /translation="MEVAVKRARELAHDDSVAAGLAEYLERHIREETHSDEPGGAARE
                     DLKALGVDADALLARLPSPKMAALVGAQYFWIFHYHPVAILGYLELEAYHPRPDTVER
                     LIEKTGLPRAGFRQLVLHAKLDAVHASDLHRVLDSLPLEPQHEQLIGLSAVHSMSLLI
                     DAMLDVVQDGA"
     gene            7867..8247
                     /locus_tag="VF225_10860"
     CDS             7867..8247
                     /locus_tag="VF225_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7145796.1"
                     /translation="MIDVQRVDYIRIPVTDMQRANQFYGEVLGLERNPNSPDDDWVEY
                     ETGNVTLAVMTPHTHDSEFTPLPPGTIAFRVPDVAEAKAKLEAAGVEVNDMWDSGVCH
                     GAGFRDPDGNAILLHHRYKPYEKT"
     gene            complement(8368..>8562)
                     /locus_tag="VF225_10865"
     CDS             complement(8368..>8562)
                     /locus_tag="VF225_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145797.1"
                     /translation="DTESVSSPLVVAISEHRTAEGELSEVLPNGALRTYWVKAVPFFD
                     AGRYLGAATYVTVVAERPSV"
BASE COUNT         1402 a         2827 c         2885 g         1448 t
ORIGIN      
        1 gttcaaggtc gtcgggctcg tgcgtccgcc actcggcggt caaacggcag acgtctacat
       61 cccgctcgcc cagctgcaga agctggccgg ccagaaggga ctcgcgaacg tcgtcctcgt
      121 ccgcgcgacg agcagctccg acgtcgccaa ggtgcaacag gagatcgagc aggctctcgg
      181 ctcgggcgcg caggtcgcaa gtgcaaagca ggtcgctgct tcgatcaacg gctcgctcgt
      241 cgacgcggcg aacctctcgc acaacctcgg cctcgtgctt gcgatcattg ctgctgtcgc
      301 agcgttcctg gtggcagccc tgttgacgct gtcgtcgatc ggcaagcgcg ttcgtgagat
      361 cgggacgctg aaggcgctcg gctggtcgca gcggctcgtc gtccggcagg tgctcggcga
      421 gtgcctcgcc accggggttg ccggcggcct cgtcggtgtt gtcctcggcg tgctcgcggc
      481 cgctacgatc ggcgcgttcg gcccaagcct cacggcgagc tcgacgaccg gcgcggccgg
      541 cgccaacgac gtcctcggac tcggccaagc gcttgctcgc acggcgtcga cgtccgtttc
      601 cttgacggcg cctttgagcc tttccctgct cctcttcggt ttcgcgctcg cactcgcagg
      661 cggactgatc gccggcgggg ccggagctct gcgcgccgcg cgactgcgtc cggccgacgc
      721 gttgaggcag atggaatgac aggcgatctc ctctacgacg tccgcaatgc gacccgaagc
      781 tttcggcgcg gggaggcgac catcaacgcg ctcagcggca tcgacctgac gatcgagcac
      841 ggagagatcg tcgcgctcgt cggcccgagc ggttccggaa agacgacgct gctgcagctg
      901 ctgggcgcgc tcgaccgccc gacgtcgggg cagatcttct tcgagggacg cgacctgaac
      961 gccctcgccg acaacgagct cgcagacctc cgcttgcgtg cgttcggctt cgtcttccag
     1021 acgttcaatc tcattccgac gttgaacgcg ctgcaaaacg tcgaagcgaa gctcgcgccc
     1081 acgggagtac cggaagacga gctccgagcc cgatcgcttg ccctcctcga ggaagtggga
     1141 ttgagcgacc gcgccgacca tctcccttcg cagctctcgg gtggagagca gcagcgtgtc
     1201 gcaatcgcgc gtgcattgtc caccgagccg cgggtcgtgc ttgcagacga gccaaccggg
     1261 aatctcgact cgcgcaccgg cacggaggtc gttgacttgc tcacttcgct ctcggaggag
     1321 cggagacaca cggtgatcct cgtcacgcac gatgccgaga tcgcggagaa ggcgcaccgc
     1381 tcgatccgga tgcaggacgg gcgtctcctg gaacccacgc ccgcctaaag ggcagtccac
     1441 cctggcaggc tgacggcgtg actcacgccc tgatcgtcac gctggcagcg ctcggtgttg
     1501 tcgggctagt cctcgccggg ctcctgcttc tcaccggctt gctctggctg tccggccacc
     1561 gcctgccgtt gcgcacattc cgacgttggc tctggggtta cgagctctgg ctggcgttcc
     1621 tcgtcgccgc agtggcgacc gggggcagtc tcttcttctc cgaggttgcg cacttcgtcc
     1681 cctgcgagct ctgctggttt cagcggatct gtatgtatcc gctttcgatc acgacgctcc
     1741 tgatggcgct cgcggacgag catcgcgcag ccaggtacct gctcccactg ccgctcgtcg
     1801 gcgccggcgt ctcgacgtac caccttctcg tcgagaacgg cgtcgtcaag caggcgcagg
     1861 cttgcctgct ctccgcgcct ggcgggtgcg cgacgaagtg gatcaacgag ttcggcttcg
     1921 tgacgatccc gacgctcgcg ctgaccgcgt tcgtcctcgt tttcgccgct ctgttgttca
     1981 gcgcgatcga gcccgtggtc ccgacagcgg aagcgaaggc ggagacgtag ccgacgccgc
     2041 taccttgatt acagatgcct agcggaaaac agagcaagcg gcagcgcggc gcagccagga
     2101 ccgggccccc gcccgtccag aggaaaggcc ggcgccggca ggtgtcgcgg cgcgcccttc
     2161 tcaccgccgc ggcggtgatc gtgctcgccg cgattggcct cacgctcgga gtcgttctca
     2221 gtgccggatc gtccaaggcc tcgacgccgg cgcgtggctc gctcgtgaat gccttgccgg
     2281 gcgccgccga cgtccagcgt ctcctctcag ggatcccgca gagtggcaac gtcctcgggt
     2341 cgaggtcggc gccggtgacg gtcgtcgagt acgtcgacct gcagtgtcct tactgccagc
     2401 agttcgagac tcaggcgatg cccgggctaa tcggacagta cgtgcgcacc ggcaaggtga
     2461 agatcgaagc tcgcccgatc gccttcatcg gcccggactc gcagcgcggc cgcgccgccg
     2521 caatcgcggc aggcgagcag aacaagctct tcaacttcgc gcaagtcctc tacctgaacc
     2581 agggcagcga gaacacaggg tggctgaacg accagatgat cgagtctgca gcctccagca
     2641 tccccggcct cgacgtgccg cagcttctct ccacccggag ctccgcgacc gtcaacacgc
     2701 gcgctgacgc cttcgaccag caggcggtgg cagacaacgt gcaggcgacg ccgacgatcc
     2761 tcgtcggcaa gaccggcggg acgctgcggc cggtggcgct caagtcgccg accgacggcc
     2821 agtcggtggc gtccgcgatc aacgcggcac ttcgcagcta gccgccccgt tccgactcgg
     2881 tcctgtccgg ccagatccgc tagacgcgtg gggcgggcat agttacgagg tggatcgcga
     2941 atccgtcacc aacgcgcctg gagcgttcaa cgccaaccag ggcgagaggg catgatcgag
     3001 gacatccgcc ccgacctggc gcgctgggtg acaccgcacc cggagtggaa gcccgaggag
     3061 gactacctcg acgagtcgta tcgtgaagtc gcctccgcgc tgcacgtcac gccggacgcg
     3121 ctcgtcctcg tcgacccgct cgtgcccgat tcgttgtggc ccgaactcga cgctcggatc
     3181 gaagcgagtg gaaagccggt ggtcgtcctc accacgatcc actggcacgc gcgcagtcgc
     3241 gacgcggtcg cgaagcggta cgacgcccgg ctgggtggcg agcctcaggg cgtgcgcgcc
     3301 ttcgacgcag ggcgccagga cgaggtcgcg ctctggctcg agggagcgcg ggccgtcgtc
     3361 ctcgccgatg ccgtggtcgg ggaccagcac ggtggactgc ggctgtcccc ctggttcaag
     3421 ttcgaggcgg agcaggagcg gacgcgtctg gcgcttctgc cgctgctcga cctgccgatc
     3481 gccatcgtgc ttcccgccca cggcaacgcc gtcctggagg atggaaagca ggcgctcgag
     3541 aaggcactcg caagggccta cacataaggg cacggaacac gagaacgcac ctacacggtc
     3601 agcacgccga ccggaatcgt ctggcgttga cgcttacgtc cgcgtaagcg gatctcgaac
     3661 tcggtatcgg gttcggtttg cggcaaaact cgacctcgtc cttggtcgtg gccggtcgta
     3721 aactcgactt atgccgcact gtccgaactg tggccattac agcgcgaagg cgttcgagtt
     3781 ctgcgcgcgg tgtggttcgc caacagcgac cgtctcgtcg cgggagcagc gcaagcgcgt
     3841 cacggtcttt ttctgtgacg tcattgcttc gaccgcgcta ggagaagggc tggatccgga
     3901 aagccttcgt cgcatgctga cgaggtactt cgagaccgcg cagcaggtga tcgaggcgca
     3961 cgggggaacc gtcgagaagt tcatcggcga cgcggtgatg gcggtattcg gcgtgcctgt
     4021 gttgcacgag gacgacgcgc ttcgcgcggt gcgcgccgcg cgcgtactgc gcgacgcgat
     4081 ggtgtcgctc aacgaggagc tcgaacgcga atatggaaat cggttggtgc tgaggatcgg
     4141 tgtcaacacg ggtgaggtgg tgaccgggac cgaagagcgg ctcgcgaccg gcgctccggt
     4201 caacctcgcc gctcgcttgg agcaggccgc cgcgcccggc gagatcctcc tcggcgccga
     4261 gacactcgca ctcgttcggc acgcggtcgt tgccgagcca cttgcgccgc ttgccgtgaa
     4321 ggggaagaga gagccggtgt ccgcgtggcg gctcctggca gtccgcggtg agaccggttt
     4381 cgcacgtcgc ttcgatgtcc cgatgctcgg tcgggagacc gagcttcgcc gcctgaggga
     4441 ggcgttcgag cagacgcagc gggatcggtc gtgccagctc gtcacgattc tgggagctgc
     4501 aggagtcggc aagtcacggc ttgcccacga gttcctggca gcgctcgacg gcgtcgccgt
     4561 gctccgtggc cggtgcctct cgtacggcga gggcatcacc tactggccag tggtcgagat
     4621 cgtgcgccaa ctcgaaccgc ggcttgcgca gctcccgctc gatggggagg tgctcgcgac
     4681 gcttcggggc ttcctcggtc cggacgacgc gatggattca atcgaagaga tcgcgtttgc
     4741 cgtccggcgg ctgctcgagg cggccgcccg cgagcttccg ctcgtgtgcg tcttcgatga
     4801 catccagtgg ggcgagcctg cctttctcga gctaattgag caggtcgcgg ttctgtcgcg
     4861 tgacgcgggc ctgttgctct gctgcctcgc gcgaccggag ctgctggagc ggcatccggc
     4921 ctggggcggg ggcatgctga actcggcaac tgtcgtcgtc gagcccctct ccggtgacga
     4981 gaccgatcag ttgatcgagc acctcgcgac ggactccctg cgcaccggtg cgctgaaggg
     5041 acgaattcgg caagcggccg agggcaaccc cctcttcgtc gaggagatga tcgcgcttct
     5101 gcgcgattcg ccggagcggg acgtctcggt gccgcctacg atccacgcac tcctcactgc
     5161 ccggcttgat cagctcgacc cggacgagcg agcgttgctg cagcgagctg cggtggaggg
     5221 ccgcgttttc catcgcggtg ccgtgcaggc gctcgcgccg gaggagacgc agatcggctc
     5281 gcgcttggcc gcgctggtgc gcaaggagct cttgggtccg gaccggccgc aattgaaacg
     5341 cgacgacgcc taccgtttcc gccacctgct gatccgcgac gtcgcttacg actcgctgcc
     5401 gaaggcgacc cgcgcagagc tgcacgagcg cctggccgac tggctcggca agcgcgcctc
     5461 tgagctggtc gaggcggacg agatccttgg ctaccacctc gaacaggccg ttcgctatcg
     5521 gctcgaactt cgaccgctcg acgactacgg gcgcgaactc gggattcgtg cggcggggct
     5581 cctcgcgggt gcaggggcgc gtgcgcttgg gcgaaacgat gtgggcgccg cgctcaagct
     5641 gctccggcgc gcggtcgcgc tccgcccgcc ggacgaccca gcggtcgcgc tccggctcga
     5701 tctcagccaa gcgctcctgt tttcgggtca gttcgccgag gctgaggaag ttacgcagga
     5761 ggccgccgcg cgtgcggtcg aaaccggcga tcaatgcggc gcgatgcgcg cccggctgat
     5821 gactctgcgg atctcgacgc agacgcccag tcaggaaggc tccgagacct ctccgagcga
     5881 cgagctcctt gcactcgccg aagaggcgtt gccgctcttc gagcacgtgg gcgacgaggc
     5941 ggctttgacc gaggcctggc tggcgattgc gtgggcacag ctgatccgtt gcggttgggg
     6001 tgaaatgctc gccgcggtcg accatgcact cgatcacgcg cgacgggccg gctacgcgcg
     6061 ctgggaaaga gaacttcccg cgtggaagag cactgcgctc tttcacggcc ccactccggt
     6121 cgacgaggtc ctggactggc acagagaaca gcagtcccag cacccaatcg ccctgaatga
     6181 ccaggctgtg ctcgaggcca tgcgcggcgc cttcgaccgg gcacgtgcgt tggcggctgc
     6241 aggacacgcg agcgcggaag cactcggcca gaccttctcg ctggccgccg aagggatgat
     6301 cggatgggag gtcgagacac tcgcgggcga cccctccgcc gcggagctga acgccagacg
     6361 gagctgcgag ttgctcgagc agctcggcga cactgccgtc cgctccctcg cgagcgggca
     6421 gctcgccgaa tcgctgtatg cgctcggccg tctcgacgaa gcgcggcagt ggacggacgt
     6481 ggccgaggag ttgagcacga acgacgacgt cgtgtcgcag atggtgtggc ggcaggtgcg
     6541 cgcgaaggtc ctcgctcgcg acggcaagca tgcggaggcc gagtcgctgg cccgcgaggc
     6601 cattcgcctc gcggaaagga cggacatggt caacaggcag ggacacgcac gtgcagacct
     6661 ggcagaagtg ctcacggagg cggggcggaa ggagcaagcc gccgccgaac tcgacgtggc
     6721 attggcttat tacgagcgca aggggaacct cgtcgctgcc gcgaacgctc gtcgcaggct
     6781 tgcagagctc agggggcgcg ctgtcgtaaa acctcgcccc gggggttaac atcgtgcggt
     6841 ctcgctcgag gcgcggcgac tgaagaccgc ggaaaggagg tcgtatggaa tccggctatg
     6901 agaacgacaa cctcgagagg ctcgagaagg aggggatgtt ccggaagtct ccccttccgg
     6961 agaagtacca agaggtcata aacgaccttc ccagggagtc agtcgacgct cttatttctg
     7021 tcaagcagcg ctttgatcgc gccggacttt ctccagagga gtcggagttc agggagtaca
     7081 tccactggtt ctagcttcgg atggggagct gaccgccagc gcccgtctcg cccgaacgat
     7141 cgagctcatc caccccttca tcggccggcc cgcgcgcctg ctgttggaac acccgcgcgc
     7201 gcgcgagcta ttgccgcggt atctggctgc tggttacccc ctcgcgagca cgatggtccc
     7261 gcttatggag gttgccgtga agcgggcgcg cgagcttgcg cacgacgatt ctgtcgccgc
     7321 gggactcgcc gaatatctgg aacggcacat tcgggaggaa acgcacagtg acgagcccgg
     7381 cggcgcagct cgcgaggatc tcaaagcact cggggtggac gcagacgcac tcctggcgcg
     7441 gctgccgtcg ccgaagatgg ccgcgctcgt cggcgcgcag tacttctgga tcttccacta
     7501 ccaccctgtc gccatcctcg ggtacctgga gctcgaggcg taccatccgc ggcccgacac
     7561 ggtcgagcga ctgatcgaga agaccgggct cccgcgcgcg ggcttcagac aactcgttct
     7621 gcatgcgaag ctcgatgccg tgcatgcgag cgatcttcac cgagtgcttg actccctccc
     7681 gcttgagccg cagcacgaac agctgatcgg attgagcgcc gtccactcga tgtctctcct
     7741 gatcgatgcg atgctcgacg tcgttcagga cggggcatag atttcggcca tggccgtaag
     7801 cgttcgaacg ctgcagaacc tcgttggcgg cgagtgggtc gactctgacg cgggaggtac
     7861 gggcccgtga ttgatgtgca gcgagtcgac tacatccgca tcccggtgac ggacatgcag
     7921 agggcgaacc agttctacgg agaggtactg ggcctcgaga ggaacccgaa ctcgccggac
     7981 gacgactggg tcgagtacga gaccggcaac gtgacgcttg cggtgatgac accgcatacc
     8041 cacgactccg agttcacgcc gttgccgccc ggaacgattg ctttccgcgt ccccgacgtg
     8101 gccgaggcga aggcgaagct ggaggccgcc ggcgtggagg tcaatgacat gtgggactcg
     8161 ggcgtctgcc atggggccgg gttccgcgac ccggacggca acgcaatcct cctccaccac
     8221 cgctacaagc cgtacgagaa gacctgacgc acatacggaa gtgattgaag atcctggagc
     8281 gggcaagatc ggtgatctcg tagcgcaagg gctcgatcgc gtcgttcatc gtcgcgctcg
     8341 agcgtttcat caaacccgcc tacgaggtca aacggacggt cgctctgcaa cgactgtcac
     8401 gtacgttgca gccccgagat atcgcccggc gtcgaagaac ggcaccgcct ttacccagta
     8461 ggtgcgcaac gctccgtttg gcagcacctc cgacagctcc ccttccgccg tccgatgctc
     8521 tgagatcgcg acaacgagtg ggctgctgac agattccgtg tc
//
LOCUS       DASVEU010000259         9377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_771923,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000259 DASVEU010000000
VERSION     DASVEU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771923"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VF225_10870"
     CDS             complement(<1..1062)
                     /locus_tag="VF225_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7145798.1"
                     /translation="MGVELAPGQVERLLELDRKFGREGLTFDDVLLVPAESRVLPNEV
                     STATRLTRGIAIEIPIVSAAMDTVTEARLAIALAREGGIGIVHRNLSIDAQMAEVDKV
                     KRSESGMIVEPVTLPRDAVVSDALELMATYHISGVPITDETGLLVGILTNRDLRFAPD
                     ASQPVSALMTSRDLVTAPLGTTLAEAEAILHRHKIEKLPVVDSEGRLKGLITVKDIQK
                     KIEFPKATKDERGRLRVGAAVGVGPDAWERAEALVDAGVDVLVVDTAHGHSASVPEMV
                     RRIKSEFDVEVVAGNVATAEGTEALIAAGADAVKIGVGPGASCTTRVVAGVGVPQVTA
                     IYESAQVAAQHDVPLIADGG"
     gene            complement(1091..2044)
                     /locus_tag="VF225_10875"
     CDS             complement(1091..2044)
                     /locus_tag="VF225_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX7145799.1"
                     /translation="MIVTVTLNAAIDRTLTVPNFQLGQRHRASQGLTLAGGKGINVAR
                     ALKRLDVPVVATGLAGGRTGTRIVEELTSEAILNDFVRIADDSRTSTAVVDPTAATYT
                     EINEWGPHVEPEELAMLLEKISYLARGADMVVFAGTLPRGVDDAFYGEAIRDLNRRHV
                     PAVLDSEGPPLRLGVEAEAFLVTPNQREAEGLVGQELGDDEDYMMALDRIADMGARNV
                     LITAETRSFGLFRAERKRTLFRADAPLVEPVSAVGSGDVLLAAFLAARVADKPLEDSL
                     KIAVAAGAASTLEVGAGRFEPREATRLQSSVHVRVLEPVAG"
     gene            complement(2041..3174)
                     /locus_tag="VF225_10880"
     CDS             complement(2041..3174)
                     /locus_tag="VF225_10880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7145800.1"
                     /translation="MILRIWVAALFAALAFVTAAHAAATPPPPVLARAFIVENPATGE
                     VLAQHGAWTRVPIASLTKLMTVIVALDHARWNAVVRVRADAAAVGESTVHLRAGERMR
                     VGDLVKAALIQSANDAAVALADYVGRGDRQAFVSMMNAKAQELGLERTHFIRPDGLDV
                     RGHYSTAKDVTTLAEFAMRNPRIRATVRLRNDTIPGGRHLHTWNDLLGVFKGLYGVKT
                     GHTGTAGWCEVAAVRRNGVTLYTTVLGSPTRSQRNTDLASLLRWGISRYRPVWLVPPG
                     HVYLEASVGYGKGSVPIVAARQVARAVRIDKPLVERVVAPNALELPVERGQQVGEVRV
                     YSGRRLVARQPLIADRSVAKPGFGGRAGFYAGRTLTHVKDWFS"
     gene            complement(3217..3606)
                     /locus_tag="VF225_10885"
     CDS             complement(3217..3606)
                     /locus_tag="VF225_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX7145801.1"
                     /translation="MDPRRRKELARYGAPAAFLAAVTIAVILIKSGLSGGSGSTTTVG
                     LFPTTTRTATTTTATTTKLVLTTPQASTATTTETTTPGAEYYAVKSGDTLGSIAQKYG
                     TTVDELMALNPGIDPTALLIGQKLRVK"
     gene            complement(3617..4525)
                     /gene="pyrF"
                     /locus_tag="VF225_10890"
     CDS             complement(3617..4525)
                     /gene="pyrF"
                     /locus_tag="VF225_10890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7145802.1"
                     /translation="MAVVATTNFADRLAEAVERKRSQLVVGLDPRPDLLPVELKGEAH
                     LGRAQAADACARFCRGLVDAVAPYVVAVKPQVAFFEALGSDGVRALEDVCAYSRAAGL
                     QVIADAKRGDIGSTARAYVAAYLESANGKPPLADAVTVNPYLGRDSLDPFLAACRRDG
                     AGIFCVVKTSNAGGAEVQDLVLSDGRHVWHQVAELVAEWGADLVGEHGLSAVGAVIGA
                     THPRAVGEARRLLPRAILLLPGVGAQGATPADVARAFTSGPASALVNVSRDVIYAFRV
                     SGMDWRSAAAAEASKYAREVWAVSGW"
     gene            complement(4608..4901)
                     /locus_tag="VF225_10895"
     CDS             complement(4608..4901)
                     /locus_tag="VF225_10895"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7145803.1"
                     /translation="MTKQEFVNEIARRADLSSRDAAKAVDAFLDAITDTLQGGGEVAF
                     TGFGKFVTQRRAERQGVNPRNPTQKVTIPAATVPKFTAGSQLKAAVKSGGAGA"
     gene            complement(5043..5576)
                     /gene="pyrE"
                     /locus_tag="VF225_10900"
     CDS             complement(5043..5576)
                     /gene="pyrE"
                     /locus_tag="VF225_10900"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7145804.1"
                     /translation="MTDDELKLALREHAYLEGDFLLRSGKRSRYYLDKYRFETRPDIL
                     APLGDRIARAVHDFDPEATRLAGPELGAVALAAAGSLASGLPFLIVRKAAKEYGTSNR
                     LEGVYTKGETVCLVEDVVTSGGALLEAVEALRNAGLTVRTAVCVVDREEGGADALARQ
                     AVRLRPIFRAGEILEAV"
     gene            complement(5573..5779)
                     /locus_tag="VF225_10905"
     CDS             complement(5573..5779)
                     /locus_tag="VF225_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145805.1"
                     /translation="MRRSIAALLGVVGGMLAGAAFIRRQSAHRERADLYYEDGSMLSL
                     TNGSPGADRLLPLARDVIRKTRGT"
     gene            complement(5769..6233)
                     /locus_tag="VF225_10910"
     CDS             complement(5769..6233)
                     /locus_tag="VF225_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7145806.1"
                     /translation="MDAQQALADLTEISSQIQAAVVFDDTGKVAASTLGDSLRADELA
                     QAAGGLLAAAEAVKAGESALTQLEVATGEGSVFVVRAGKTVIAATTGSNPTAGLVFYD
                     LKSALRNVKPKPAAEKKPAGTTKPAAAKKTATAAKKKTAARKPATRKKPDAP"
     gene            6326..7159
                     /locus_tag="VF225_10915"
     CDS             6326..7159
                     /locus_tag="VF225_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7145807.1"
                     /translation="MRVRRRDLVQKFYADRGPHLAAMIAYFALLSFVPLTFISLALLG
                     LTGRADESSFLVREIKKTLPGTPIDQIINLVHTVQDNAAALGIIGGLALLWTSLSLFS
                     VLESALNIVYGRPNRSFLHGKGLAFLLMVGSLVTLFVALLAGSLGVTALQEYLPGVVS
                     NAPTAYALSIGVSLIGVFVFLVSCYYVLTNEDDLTLREVLPGAIFAAVLLEATFQVLP
                     LYQRYADLNPALRAFGAPAILLVWLYVMANVIVFGAELNWWLTHRAAQRQEDEQLAGL
                     A"
     gene            complement(7142..7711)
                     /locus_tag="VF225_10920"
     CDS             complement(7142..7711)
                     /locus_tag="VF225_10920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX7145808.1"
                     /translation="MDGLRARLASRNPNKARELEQLLPNWRIEPLEADDYPVEDGATY
                     YANARAKALFGRTRAEPDEWALGEDSGIEVAALGGGPGVRSARSGGDDPVGWLLDALE
                     DQEDRHARYVSELVLISPAGDELRGTGTLAGSIAVEPRGTEGFGFDPIFIPAGEEQTV
                     AELGNGWKRENSHRARAALALLGVLGEAG"
     gene            complement(7699..8406)
                     /locus_tag="VF225_10925"
     CDS             complement(7699..8406)
                     /locus_tag="VF225_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7145809.1"
                     /translation="MLQLASSQLPTLNWDESLLRLALAALLGGLIGVERELREREAGL
                     RTHLLVSLGSALFTIVGAYGFHAFLDSGANVVRADPTRIAAQIVTGIGFLGAGAIIRQ
                     GLSVRGLTTAATLWVVAAVGLASGAGYYSAAVITTALVLLALWPLRIMAYRILVRFRP
                     EDGRLLVALPAGQPPGEVIDEVEKTGARIASLEVSQEGDRRRLEFDVVLPRDTPAARI
                     VARIADLENVTEVRWTD"
     gene            complement(8387..9094)
                     /gene="rph"
                     /locus_tag="VF225_10930"
     CDS             complement(8387..9094)
                     /gene="rph"
                     /locus_tag="VF225_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7145810.1"
                     /translation="MREGRRTDELRPLTVELDYLEQPHGSVLWSQGKTKVLCTAMIDD
                     EVPRWLRNKGKGWMTAEYSLLPASTGERIAREASRGKQGGRTVEIQRLIGRALRGVVD
                     FEALGERTVWLDCDVLQADGGTRCAAITGAYVAARRALDKFGLSKALSGSVAAVSVGI
                     VDGEALLDLDYSEDSQAEVDMNVVMTGDGALVEVQATAEKTPFSRARLDELLELAAGG
                     IEELALVQEEAVAAARV"
     gene            complement(9091..>9377)
                     /locus_tag="VF225_10935"
     CDS             complement(9091..>9377)
                     /locus_tag="VF225_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145811.1"
                     /translation="TVILGCTHYPIISPILQRVFGRDVTLIFSADETAREVAETLARK
                     HVENDPAREGSYRFITTGDPDVFRETGARFLQLPIREVEHVELLELERVA"
BASE COUNT         1470 a         3343 c         3047 g         1517 t
ORIGIN      
        1 tccgccgtcc gcgatgagcg gaacatcgtg ttgcgctgcg acttgcgcgg attcgtagat
       61 cgccgtgact tgagggacgc cgacgccggc gaccacccgg gtcgtacaac tcgcacccgg
      121 tccgactccg atctttaccg cgtctgcacc tgcagcgatc aaagcttccg ttccctcggc
      181 cgtcgccacg ttgcccgcaa cgacctcgac gtcgaactcc gacttgatcc gccgcaccat
      241 ctccggcacc gacgccgaat ggccgtgcgc agtgtcgacg accagtacgt cgacaccagc
      301 gtcgacgagc gcttccgccc gctcccacgc atcgggaccg acaccgactg cggcgccgac
      361 acgtaatcgt ccccgctcgt ccttcgtcgc cttggggaac tcgatcttct tctggatgtc
      421 cttgacggtg atcagcccct tcagccgtcc ttccgagtcg acgaccggga gcttttcgat
      481 cttgtggcgg tggaggatcg cctccgcctc tgcaagcgtc gtcccgagtg gcgccgtcac
      541 caggtcgcgt gatgtcatca acgcggacac cggctgcgac gcgtcgggtg caaaacggag
      601 atcgcgattc gtcaggatcc cgacgagcag gcccgtctcg tcggtgatcg gcacaccgga
      661 gatgtggtac gtcgccatca gctcgagcgc atcggaaacg accgcgtccc gtggcaaggt
      721 gaccggctcg acgatcatcc cggactccga gcgcttcacc ttgtcgacct ccgccatctg
      781 cgcgtcgatc gacaggttgc ggtggacgat cccgatccca ccttcacgcg ccagcgcgat
      841 cgcgagcctc gcctcggtga ccgtgtccat cgcagcggag acgatcggga tctcgatcgc
      901 gatgccgcgc gtcagtcgcg tcgcggtcga gacctcgttc ggcagcacgc gcgactcggc
      961 cggaacgagc agaacgtcat cgaacgtcag cccttctcgg ccgaatttcc ggtcgagctc
     1021 gaggagccgc tcgacctgtc cgggcgctag ctcgactccc atcgcgtcac gctagcaccc
     1081 cgccaagaca tcagcccgcg accggctcga gcacccgcac gtggaccgag ctctgcagcc
     1141 gagtcgcctc ccgcggctcg aagcgcccgg ctccgacctc gagcgtcgag gccgccccgg
     1201 cggccaccgc gatcttcagc gagtcctcga gcggcttgtc tgcgacccgc gcagcaagga
     1261 aggccgcgag gaggacgtcc ccggagccga ccgcggacac aggctctacc agcggagcgt
     1321 cggcgcgaaa cagcgttcgc ttccgctcgg cgcggaagag cccgaagctg cgggtctcgg
     1381 cggtgatcag cacgttgcgc gcgcccatgt ccgcgattcg gtcgagcgcc atcatgtagt
     1441 cctcgtcgtc cccgagctcc tggcctacga gtccctcggc ctcacgttga ttcggcgtga
     1501 cgaggaacgc ctctgcctcg acgcccaggc gcaacggcgg cccttccgag tccaggactg
     1561 ccggcacgtg gcgccggttg agatctcgga tcgcctcccc gtagaacgcg tcgtcgacac
     1621 cccgcggcag agtgccggcg aacacgacca tgtcggcgcc gcgcgcgagg tagctgatct
     1681 tctcgagcag catcgcgagc tcctcgggct ccacatgggg cccccactcg ttgatctccg
     1741 tgtaggtcgc cgccgtcgga tcgacgaccg ccgtcgaggt gcgcgaatcg tcagcgatcc
     1801 gcacgaagtc gttgaggatc gcctcggagg tcaattcctc gacgatgcgc gttcccgtgc
     1861 gcccgccggc gaggcccgtc gcgaccacgg ggacgtccag ccgcttcaga gcgcgcgcga
     1921 cgttgatccc tttgccgccg gcgagcgtca gaccctggct ggcgcggtga cgctggccca
     1981 gctggaagtt cgggaccgtc agtgtccgat cgatcgccgc attcaacgtc accgtgacga
     2041 tcacgagaac cagtccttca cgtgggtgag ggttcggccc gcgtagaagc cggctcgtcc
     2101 accgaagccc ggcttggcca cggaacggtc ggcgatcaac ggttgtcgtg ccacgagccg
     2161 acgtccagaa tagacgcgga cttcgccgac ctgctggcct cgttcgaccg gcagctcgag
     2221 cgcgttcggg gcgacgacac gctcgacgag cggcttgtcg atgcgcacgg cccgcgcaac
     2281 ctgccgggcg gcaacgatcg ggaccgagcc ttttccgtac ccgacgctcg cctcgagata
     2341 gacatggcca ggaggcacga gccacaccgg ccggtaccgc gagatgcccc agcgaaggag
     2401 agacgccagg tcggtgttgc gctgcgaacg tgtggggctc ccgagcacgg tcgtgtacag
     2461 cgtgacaccg ttgcggcgca ccgccgcgac ttcgcaccag ccggccgtgc ccgtgtgtcc
     2521 cgtcttgacg ccatagaggc ccttgaagac gccgagcagg tcgttccacg tgtgcaggtg
     2581 ccggcctccg gggatcgtgt cgtttcggag ccgaacggtg gcccggattc ggggattgcg
     2641 catcgcgaac tccgcaagcg tggtcacgtc cttggccgtg gagtagtggc cccgcacgtc
     2701 cagcccatcc ggccggatga agtgcgttcg ctcgagcccg agttcctgcg cctttgcgtt
     2761 catcatcgag acgaacgctt ggcggtcgcc gcggccgacg taatcggcga gcgcgacagc
     2821 agcgtcgttc gcgctctgaa tcaacgccgc tttgacgaga tcgcccacgc gcatgcgctc
     2881 gccggcgcgg aggtgcacgg tcgactcacc gactgcggcc gcatccgccc ggacccgcac
     2941 caccgcgttc cagcgcgcat gatcgagggc gacgatgacc gtcatcagct tcgtgaggct
     3001 tgcgatcggc acacgcgtcc aggctccgtg ctgcgcaagc acctcgccgg tcgcgggatt
     3061 ctcgacgatg aacgctcgcg caagaaccgg cggcggcggc gttgctgccg catgtgcagc
     3121 ggtgacgaag gcgagcgccg caaagagggc ggcgacccag atccggagga tcacgagcgg
     3181 gaaggctagc gcgcccctcc cggtgccggt agggcgctat ttgacccgga gcttttggcc
     3241 tatgagcagc gctgtcggat cgatgccggg attcaaggcc atcagctcgt ccacggtcgt
     3301 gccgtacttc tgtgcgatgg agccgagcgt gtcgccgctc ttcaccgcgt agtactccgc
     3361 tccaggcgtc gtcgtctccg tcgtcgtcgc ggtggaggcc tgtggggtcg tcagaacgag
     3421 cttcgtcgtt gtcgccgtcg ttgtcgtcgc ggtgcgcgtc gtcgtcggga acaggccgac
     3481 agtcgtcgtc gatcccgagc caccgctcaa gcccgacttg atcaggatca cagcgatcgt
     3541 gacggccgcg aggaacgccg ccggagcgcc gtaccgcgct agttccttac gcctgcgcgg
     3601 atccatggtt tgctccttac cagccggaga ctgcccacac ttcgcgcgcg tacttcgaag
     3661 cctcggcggc ggccgcgctg cgccagtcca tgccgctgac gcggaaggcg tagatcacgt
     3721 cccgcgagac gttcacgagt gcgctcgccg ggccgctcgt gaacgcgcgc gccacatctg
     3781 caggcgtcgc cccctgggcg ccgacaccag ggagcagcaa aatcgcacgt gggagcagcc
     3841 tccgggcctc gccgacagca cggggatgcg tcgcaccgat gacggcgccg acggccgaca
     3901 ggccgtgctc cccgaccaga tcggcacccc actcggccac gagctcggcg acctggtgcc
     3961 agacgtgccg cccgtcggag agaacgaggt cctgcacctc cgccccgccg gcgttcgacg
     4021 tcttgacgac acagaagatg cctgccccgt cgcggcggca cgcagcaagg aacggatcga
     4081 gcgaatcacg gccgagatac ggattcacgg tcaccgcatc ggcgagtgga ggcttgccgt
     4141 tcgccgattc cagatacgca gccacgtacg cgcgcgcagt cgagccgatg tcgccgcgct
     4201 tcgcgtccgc gatcacctgc aatccggccg ctcgcgagta cgcacacaca tcctcgagag
     4261 cgcgcacgcc gtccgagccg agggcctcga agaaggcgac ctgcggcttg accgccacga
     4321 cgtaaggcgc gaccgcatcg acgaggcccc gacagaagcg tgcgcaggcg tccgccgcct
     4381 gggcgcggcc gagatgggct tcgcccttca gctcgacggg caggaggtcc ggccgcgggt
     4441 cgagcccgac gaccagctgc gaacgcttcc gctcgaccgc ctcggccagg cggtcagcga
     4501 agttcgtcgt cgcgacgacg gccacaacgt aaaggtactt cgccctaaag acggcgaagg
     4561 ccgcttccta ggcggccttc gcagttctcg ggcgggaggc tcagggacta ggcgccggcc
     4621 ccgccgctct tcacggccgc cttgagctga ctccccgcgg tgaacttcgg caccgtggct
     4681 gccgggatcg tcactttctg cgtcggattg cggggattca cgccctggcg ctctgcgcgc
     4741 cgctgggtca cgaactttcc gaagcccgtg aaggccacct cgccgccgcc ctgcagcgtg
     4801 tcggtgatcg cgtcgaggaa ggcgtctacg gccttcgccg catcgcgact ggagagatcg
     4861 gcgcgcctcg cgatctcgtt aacgaactcc tgtttcgtca agtcccactc ctcctgtcga
     4921 caaatcgaac cacgggcaaa gaccctaaca gcagcgcggg acccgctcaa ccatgcggtt
     4981 ttgcgggtct tttgagccga atcggacgac cctcggcgtt gttttggcac aaaaagcggg
     5041 cctcagacgg cctcgaggat ctcgccggcc cgaaaaatcg gacgcaaccg aacggcctga
     5101 cgagcaagcg catcggctcc gccttcctcg cgatcgacga cacagaccgc cgttcgcacg
     5161 gtcagtccgg cgttacgcag ggcttcgacc gcctcgagca gagcgccgcc ggaggtgacg
     5221 acgtcctcga cgaggcagac ggtttcaccc ttggtgtaca caccctcgag gcggttcgaa
     5281 gtgccgtact ccttcgcagc tttgcgaacg atcagaaacg ggagcccgga agcgagcgac
     5341 cccgcggccg cgagcgcgac ggcgccgagc tcgggcccgg ccagacgcgt cgcctccggg
     5401 tcgaagtcgt gcaccgcacg cgcgatccgg tcaccgagcg gcgccaggat gtcgggccgc
     5461 gtctcgaagc ggtacttgtc caggtagtag cgcgatcgct ttcccgaacg cagcaagaag
     5521 tcgccctcga gataggcgtg ctcgcggaga gcgagcttca gctcgtcgtc ggtcaagtac
     5581 cgcgggtctt gcggatgacg tcccgcgcaa gcggcagaag ccgatcggct ccgggggagc
     5641 cgttcgtgag cgagagcatg gaaccgtcct cgtagtagag atcggcccgc tcgcgatgcg
     5701 ccgactggcg acggatgaac gcggcgccgg cgagcatgcc gccgacgacc cccagaagtg
     5761 cggcgatgct acggcgcatc cggcttcttc ctcgtcgccg gcttgcgcgc ggccgtcttc
     5821 ttcttggcag cggtcgccgt cttcttcgct gcggcgggct tcgtggttcc ggccggcttc
     5881 ttctcagccg cgggcttcgg cttcacgttg cgcagggccg acttgaggtc gtagaagacg
     5941 agcccggcgg tcgggttgct gcccgtggta gccgcaatga ccgtcttgcc ggcgcgcacc
     6001 acgaagacgc tgccttcgcc ggtcgccacc tcgagctgcg tcagcgcgct ctctcccgcc
     6061 ttgaccgcct ccgcggcagc gagcagccca ccggccgcct gggcgagctc atccgcgcgg
     6121 agggaatctc cgagcgtgga cgcggcgacc ttccccgtgt cgtcgaacac gaccgcggcc
     6181 tggatctgcg aggagatctc ggtcaggtcg gcgagtgcct gctgggcgtc catctcgggc
     6241 caaagcatac ggcgtcgctc gcgacgccga ccggaggagg ccaccgcggc tcggccggcg
     6301 gggccgttga cgggatactc ccgccgtgcg agtgcgacga cgcgacctgg tgcagaagtt
     6361 ctacgccgac cgcggcccgc atctggcggc gatgatcgcc tacttcgcgc ttctctcctt
     6421 cgtcccgctg accttcatct cgctcgcgct gctgggactg accgggcgcg cggacgagtc
     6481 ctcgttcctc gtcagagaga tcaagaagac gcttccgggg acaccgatcg accagatcat
     6541 caacctcgtg cacaccgtcc aggacaacgc cgcggcgctg ggcatcatcg gtgggctcgc
     6601 attgctttgg acgtcgcttt cgctcttcag cgtgctcgaa tcggcgctca acatcgtcta
     6661 cggacgcccg aaccgctcgt tcctccacgg taaagggctt gccttcctct tgatggtcgg
     6721 ctcactggtg acgctgttcg tcgcgctgct cgcaggctcg ctcggcgtga cggcgttgca
     6781 ggagtaccta ccgggcgtcg tgtccaatgc gccgacggcg tatgcgctct cgatcggcgt
     6841 ctcactcatc ggcgtgttcg tgttcctcgt ctcctgttac tacgtgctga cgaacgagga
     6901 cgacctgacg ctacgggaag tcctccctgg tgcgatcttt gccgccgtcc tcctcgaggc
     6961 aacgttccag gtgctcccgc tctatcagcg ctacgcggac ctcaaccccg cgctgcgtgc
     7021 gttcggcgcc ccggccatcc tgctcgtctg gctctacgtg atggcgaacg tgatcgtctt
     7081 cggcgccgag ctgaactggt ggctcaccca ccgggccgcg cagcgccagg aagacgagca
     7141 gctagccggc ctcgcctaaa acgccgagga gcgccagcgc agctctggcg cggtgcgagt
     7201 tctctcgctt ccagccgttg cccaattcgg cgacggtttg ctcttccccg gcaggaatga
     7261 agatcgggtc gaagccgaag ccttccgtac cgcgcggctc gacggcgatg ctgcctgcga
     7321 gcgttcccgt gccgcggagc tcgtcgccgg ccggcgagat gagcacgagc tcgctcacgt
     7381 agcgcgcgtg gcggtcctct tggtcctcca gcgcgtcgag cagccagccc accggatcgt
     7441 ccccgcccga acgagccgac cggactcctg gtccaccacc gagcgccgcc acctcgatcc
     7501 ccgagtcctc cccgagagcc cactcgtccg gctctgcccg cgtccgtcca aacagtgctt
     7561 tcgcgcgcgc gttggcgtag tacgtcgcgc cgtcctcgac cgggtagtcg tcggcttcga
     7621 gcggctcgat cctccagttg gggagcaact gctcgagctc acgcgccttg ttcgggttgc
     7681 gcgaggcgag ccgcgccctc agtccgtcca ccgcacctcg gtaacgttct cgagatcggc
     7741 gattcgcgcc acgatgcgcg ccgccggagt gtcccgcggc aggacgacat cgaactcgag
     7801 gcgccggcgg tctccttcct ggctgacctc gagcgacgcg atccgtgctc ccgtcttctc
     7861 gacctcgtcg atcacctcgc ccggcggctg gccggccggc agcgcaacga gcagccgtcc
     7921 atcctccgga cggaagcgaa cgaggattcg gtacgccatg attcgcaacg gccagagggc
     7981 gagcagcacg agcgcagtcg tgatcaccgc ggcgctgtag taaccggcgc ccgacgcgag
     8041 accgactgcg gcgacgaccc acagcgtcgc ggcagtggtc aggccgcgca ccgacagtcc
     8101 ctgccggatg atcgcgcccg ccccgagaaa gccgatgccg gtgacgatct gggctgcgat
     8161 ccgcgtcggg tccgcacgca ccacgttcgc gccggaatcg aggaacgcgt ggaacccgta
     8221 cgcaccgacg atggtgaaca aggccgagcc cagcgaaacg aggaggtgcg tgcgcaagcc
     8281 ggcctcgcgc tcgcgcagct cgcgctcgac cccgatcagg ccgccgagca gcgcggcgag
     8341 ggcgagccgc aggagcgact cgtcccagtt gagggtcggc agctggctag acgcgagctg
     8401 cagcaacggc ttcctcttga accaatgcca gctcttcgat ccctcccgca gccagctcga
     8461 gcagttcgtc caagcgagca cggctgaacg gcgtcttctc cgcggtcgcc tgcacctcga
     8521 cgagcgcgcc gtcgcccgtc atgacgacgt tcatgtccac ctcggcctgg gagtcctcgg
     8581 agtaatcgag gtcgagcagc gcctcgccgt cgacgatccc gaccgacacc gccgcgaccg
     8641 agccggacaa cgccttcgag aggccgaact tgtcgagtgc acgccgcgcc gcgacgtacg
     8701 cgccggtgat cgcagcgcag cgagtcccgc cgtcggcctg cagcacgtcg cagtcgagcc
     8761 agaccgtccg ctctccgagc gcctcgaagt cgaccacacc gcggagcgcg cggccgatca
     8821 gccgctggat ctccaccgtg cgaccaccct gcttgccgcg cgacgcctcg cgcgcgatgc
     8881 gctcaccggt cgacgccggc agcagcgagt actccgccgt catccagccc ttgcccttgt
     8941 tgcgaagcca gcgcggaacc tcgtcgtcga tcatcgccgt gcagagcacc ttcgtcttgc
     9001 cctgcgacca gagcacggag ccgtgcggct gctcgaggta gtcgagctcg accgtcagcg
     9061 gccgaagctc gtcggtgcgg cggccttccc tcatgcgacg cgctccagct cgagcagctc
     9121 gacgtgctcg acctcgcgga tcggcagctg caggaagcgc gcgccggtct cgcggaagac
     9181 gtcgggatcg cccgtcgtga tgaagcgata gctgccttca cgtgcagggt cgttctcgac
     9241 gtgcttgcgc gcaagcgtct cggcgacctc gcgcgcggtc tcgtcggcgg agaagatcag
     9301 cgtcacgtcg cggccgaaga cgcgctggag gatcggactg atgatcgggt agtgcgtgca
     9361 gccgaggatc accgtgt
//
LOCUS       DASVEU010000260         6739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_965643,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000260 DASVEU010000000
VERSION     DASVEU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965643"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VF225_10940"
     CDS             <1..527
                     /locus_tag="VF225_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7145812.1"
                     /translation="TAWQGLFHHGHLQAGQSVLAHGAAGAVGTMVTQLAREAGAYVIG
                     TGRAADRQKALDLGAQEFVDLENDALEEVGGVDLVFDVIGGDIQKRSASLIRAGGTLV
                     TVVGPPEARPADGLAVDFVVESDRAQLREIVQRVRDGRLRTNIGNVATLDDAVAALNP
                     TERRNGKTIIRIRP"
     gene            1040..1393
                     /locus_tag="VF225_10945"
     CDS             1040..1393
                     /locus_tag="VF225_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7145813.1"
                     /translation="MAITQRERLLRSLYEAFNTRDVDSCLAAMTPDVDWANGWEGGRV
                     VGRDAVRDYWDRQWAAIDSSAQPIAISERPDGSIEVTVRLMARDNAGAVISDSEGRHV
                     YEFRGHLVQRMTIEE"
     gene            1592..2002
                     /locus_tag="VF225_10950"
     CDS             1592..2002
                     /locus_tag="VF225_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145814.1"
                     /translation="MTTPPAHDTRLERILGLPLADDAIRQWPQSGEVINGRERIGVVE
                     SHFEGLRLAVGRRHACGDTIVVEWSTDYGDGRVYRNVTVADMRDGHAIRVTDYWGEPF
                     EAPAWRESLADALEMPHHGVWPAVEALQADDEAP"
     gene            complement(2199..2777)
                     /locus_tag="VF225_10955"
     CDS             complement(2199..2777)
                     /locus_tag="VF225_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7145815.1"
                     /translation="MLIWSMGVSVDGFIADREGAFGWTVPDEEVFRFHIAQVRELGGF
                     LCGRRLYETMVPWETDPSMRDNEIGAVPSSAAAEFADAWCAIPKVVFSRTLDSVQGNA
                     RLAEASVAEEAAAALDATDNDVAIGGAGLAAQAIELGLVDELRMFRHPIVVGGGTPFL
                     PPVTEDVPLDLIETRTFGSRVIYERYGRARDH"
     gene            3235..3648
                     /locus_tag="VF225_10960"
     CDS             3235..3648
                     /locus_tag="VF225_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7145816.1"
                     /translation="MADPKRVVERHVETFNARDADASPWSEDADFVAPNASMHGRDEV
                     LGFLGVFWDAFPDGRLETSGLIAEGSVVAAEGRFIGTHTGVMRTPAGDVAATGRHVDL
                     RWMSSYQVRGDELASEHLYFDQAELLGQLGLMPAT"
     gene            complement(4106..4648)
                     /locus_tag="VF225_10965"
     CDS             complement(4106..4648)
                     /locus_tag="VF225_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7145817.1"
                     /translation="MSDTSTGTGKVVVNRAMSLDGFIAGPDHAMDWILDLVSAAAFPE
                     VMAATGAMLIGRGTYEVGKQMADEDTTYDGGAKFVLTHEPPDEPDPGVTFLTCDIAEA
                     VATARNAAGGKNLEILGADLASQCLQRGLVDEILVYVLPVLLGDGVRFSTPGLGRIDL
                     EPFSNTQSGPVTMLRFRVRK"
     gene            complement(5449..5521)
                     /locus_tag="VF225_10970"
     tRNA            complement(5449..5521)
                     /locus_tag="VF225_10970"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:Ala,seq:cgc)
     gene            5635..6207
                     /locus_tag="VF225_10975"
     CDS             5635..6207
                     /locus_tag="VF225_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145818.1"
                     /translation="MGILDDAIREHLELKRRHGASEEELRQQEVEALGSARREAAEED
                     SGEPAAAEAAPEPVDGNGAVPAAEEADLEPAESAEPALELEPEPSPEPVQEHPAPPIV
                     EEPEPALEEPEPEPEEPQRADEPELVEDPRRSGDTPTRGFEALEEPGEEESEDEDADV
                     LEDTPDFLQETPEHDRLWFEQKPPRDFDFD"
     gene            complement(6413..>6739)
                     /locus_tag="VF225_10980"
     CDS             complement(6413..>6739)
                     /locus_tag="VF225_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145819.1"
                     /translation="CRMVVEGANSPTTPAADEILQEKQVYVVPDVMANAGGVVVSYFE
                     WVQNLQHFRWDEREVNDRLGRIMRKAFREVSARSRESGTALREAAYEVGIERVVEAAT
                     VRGYIA"
BASE COUNT         1155 a         2257 c         2251 g         1076 t
ORIGIN      
        1 tgaccgcgtg gcagggactg ttccaccacg gccatcttca ggcggggcag agtgtcctgg
       61 cgcacggcgc agccggcgca gtcgggacga tggtgacgca actcgcacgt gaggccggtg
      121 cctacgtcat cggcaccggg cgcgccgccg accgccagaa ggcgctcgac ctcggcgcgc
      181 aggagttcgt cgatctggag aacgacgccc tggaagaggt cggcggcgtc gacctggtct
      241 tcgacgtcat cggcggagac atccagaagc ggtccgcaag cctgatccgg gccggaggaa
      301 ccctggtgac cgtcgtaggg ccgcccgagg cgcggcccgc cgacggcctg gcagtcgact
      361 tcgtcgtcga gtccgatcgc gcccaactgc gtgagatcgt ccagcgggta cgggacggac
      421 ggctgcggac caacatcggc aacgtcgcga ccctcgacga tgccgtcgcc gccctcaacc
      481 cgaccgagcg acgcaacggg aagacgatca tccgcattcg tccataagga ctcggagacg
      541 ctcctggcgc cgacgtggga gacgccggac gccaacgccg aacttggcgg cggcgtttcg
      601 gaagctcgag gtgggtgtcg cgttggtggg cacggcagag gcggtaggcc tcgtcgttct
      661 ccgagtgggc tgggggaagc tggagaggcg ccttgctctg ctacccggcc accctggcgt
      721 gaagggacga gctcacggct acccgatcgg aatagctgct tcgctccagt gcaggcgttt
      781 cgttgtcaat tggacaacga ccgaatggac ctaaccggac agctttcaaa cctctccact
      841 gatctggggg aactcctgtc gacgtcgtga cgctcgctcg ccgatgcgac atcggttctg
      901 ctccatcgcg caaagtgaga gtcggcgcgg cgtggcttca ggcttccgcg tgtcgtccgc
      961 agcagagcct gcgagtagcg tctttgcaga tcgaagggaa cctatgatcg accgccgtta
     1021 cgttcagtaa ggagaggtca tggccatcac gcaacgcgag cggctcctac ggtcgctgta
     1081 tgaggccttc aacacgcgcg acgtcgatag ctgcctggcg gcgatgactc ccgatgtcga
     1141 ctgggccaac ggctgggaag gcggccgcgt cgtaggacgc gacgccgtcc gcgactactg
     1201 ggaccgccag tgggccgcga tcgactccag cgcgcagccg atcgcgatca gcgaacgccc
     1261 cgacggcagc atcgaggtca ctgtccgcct catggctcgc gacaacgcgg gagcggtgat
     1321 ctccgactcc gagggccgtc acgtctacga gtttcgggga catctcgtgc agcgtatgac
     1381 catcgaggaa tgaccgctcc tcggaacatg caaagcgtct cgttgtctcg gtagacgttg
     1441 gcttgcgggg cgggattgag gtgcagcacg ccccagtgca actctggggc gacggcttcc
     1501 ttcgcgcaga ccggaagctt ggggctctcc tccgcgaaca tacattgctc gaaaggtcga
     1561 ccggcaccga ctgccacgat ggaggactga tatgacaact ccgccggctc acgacacccg
     1621 actcgagcga atcctcggcc tccctctggc cgatgacgcg atacgtcagt ggccgcagag
     1681 cggcgaggtg atcaacggca gggagcgcat cggcgtagtg gagtcccact tcgagggtct
     1741 ccgactcgcg gtcggccgac gccatgcatg cggcgacacc atcgtggtcg agtggagcac
     1801 cgactacggc gacggtcgcg tctaccgcaa cgtcactgtc gcggacatgc gagacgggca
     1861 cgcgatccgg gtcacggact actggggcga gccattcgaa gcgcccgcct ggagggagtc
     1921 actggccgac gcgctagaaa tgccacatca cggggtttgg ccggcagtcg aagcccttca
     1981 agccgacgac gaagcgccct gagcgtgccc tcagctgtgg ttgtcaatag tcgctccccc
     2041 tttcgcgtgc aagcggcggc gggtgcttgt cgcgcaaagc ggcaacgtcc cgcgtgatct
     2101 gcagcgttga tcccgtcggg cacatcgtcg gccctgatgt cgacgcgtga tcgggcgtcg
     2161 atgggggggt gacctcccat gtcatggtca tcgtaccatc agtggtcgcg ggcgcgcccg
     2221 tagcgctcgt agatcacgcg cgagccgaac gtcctggtct cgatcaggtc cagcgggacg
     2281 tcttcggtga ccggcggcag gaacggcgtg ccgccaccga cgacgatcgg atggcggaac
     2341 atgcgcagct cgtcgacgag gccgagctcg atcgcctgcg cggccaggcc ggcgccgccg
     2401 atcgcgacgt cgttgtcggt cgcgtcgagc gccgcagcgg cctcctcggc caccgacgcc
     2461 tcggcgagcc gggcgttgcc ctgaacgctg tcgagcgtgc ggctgaagac gaccttgggg
     2521 atcgcgcacc aggcgtcggc gaactcggcg gcggcgctcg aagggaccgc cccgatctcg
     2581 ttgtcgcgca tcgacggatc cgtctcccac ggcaccatcg tctcgtaaag cctgcggccg
     2641 cacagaaacc cgccgagctc acgtacctgc gcgatgtgga aacgaaacac ctcctcgtca
     2701 gggacagtcc acccgaacgc gccctcgcgg tcagcgataa atccgtccac cgaaacgccc
     2761 atcgaccaga tcagcatggc ctcaagctca tcgcaccgga gcgacagacg atcatcgcga
     2821 tgaagttcac cattgagcct gacatcgggc tctccttcct tctgcaggct gctctgccca
     2881 cagctagtga ccgatcgcgc cgcaccgaga tcccggagtc cttaaccgcg tgaaaccagg
     2941 agcgaagcgg cgcgtaccgc gaccttgtct ttgacaacga gcgaatggac ctaacgggac
     3001 agctttcaaa ccttccgatt gacctgaggg aactcttgtc gaaggcgtga gcctcgctcg
     3061 gtcgcggtgg ctaccgcatt gcatggaatc cagcagtctg ctcggcgtga gcgcgactgc
     3121 cggtagcagg cgaaagcgca agccgggggg gggcggcgct cgctacccga ttgtcagaag
     3181 accgtcgcag agtgcgagta tccgcgcacg gcacccgata ggaggtatgg cctgatggct
     3241 gatcccaagc gcgtcgtcga acggcatgtc gagacgttca acgcacggga cgcggacgcc
     3301 agtccgtgga gcgaggacgc cgacttcgtc gcaccgaacg cgtcgatgca cggccgcgac
     3361 gaggtcctgg gcttcctcgg tgtgttctgg gacgcgtttc cggacgggcg tcttgagacg
     3421 tcgggcctta tcgccgaagg atccgtcgtc gccgccgagg gccggttcat cggcacgcac
     3481 accggtgtca tgcgcacgcc ggcaggggac gtcgcggcca cgggacggca cgtcgacctc
     3541 cgctggatgt cgagttacca ggttcgcggc gacgagctgg cctcggaaca tctctacttc
     3601 gatcaggccg agctgctcgg ccagctcgga ctgatgccgg cgacctgagc gagcgtccct
     3661 gccttgcgcg aagagcaacg acagcctcta gagccgcatc tgcctcgttg cccgcttggc
     3721 ggatgacatc gccgaggaag cgccgagcta cgcgccgcag tccggatggc ctcgcgctgt
     3781 ccggctgctg ccttggagcg atcggaacgg caaagacgcc tgtttgcagg caatgcgttg
     3841 tcaattggac aacgaccgaa tggacctaac ggaacagctt tcaaacctct cgattgacct
     3901 gaggaaactc ctgccgatgg cgtgagcctc tgtcggtcgc cgtggctatc gcaatgcatc
     3961 cgatcgtgtg tggggatcgc cttgcggcca tccggtgctg ttgcgcctgt ctcttcgtgc
     4021 aacgagtcag gcggccccat cgcacgtccc gcctggcaaa cgagctgaga tgctgctcgt
     4081 gccgttcagg gacggagcgc cgggatcatt tgcggacgcg gaatcggagc atggtgaccg
     4141 ggcccgactg cgtgttgctg aacggttcca ggtcgatcct gccgaggcct ggggtcgaga
     4201 agcggacacc gtcgccgagc agcaccggca ggacatacac caggatctcg tcgacgagcc
     4261 ctcgctgcag gcactggctg gccaggtcgg cgccgaggat ctccaggttt ttgccgcccg
     4321 cagcgttgcg cgccgtggcg acggcttccg cgatgtcgca ggtgaggaac gtgacccctg
     4381 ggtccggctc gtccggcggc tcgtgggtga ggacgaactt cgcccccccg tcgtacgtgg
     4441 tgtcctcgtc ggccatctgc ttgccgacct cgtaggtgcc ccggccgatg agcatggcgc
     4501 ccgtggccgc catgacctcc gggaacgcgg ccgcgctcac aaggtcgagg atccagtcca
     4561 tcgcgtggtc gggaccggcg atgaagccgt cgagcgacat cgccctgttc acgaccactt
     4621 tgccagtgcc ggtgctcgtg tcgctcacgc gcttcctcgc aggactccgg cgggctggcg
     4681 ggacagacag cgagtcgtct ccgcccgagc ggcgacggga cgcgacacgt gaacgccagc
     4741 gccgcgccag acgacgtagt agccgggact atccccgagc ttggtctggt ggtgcgccgt
     4801 gacgtatctc caaaggtcgt cgaggccgat gtctaacacc tcgctgtacc acgcactcag
     4861 cgatgtcgaa gttgcccgac gcattcggcg acccatccga gagtttgcga gtggcaccgg
     4921 ccccggggcg ccacggggtg ctcggcagag agtacctggg atgggctcat ttcgacggga
     4981 atagcacagc acccactcgt cccccacgcc gaacccctcg cgggcactcc acctgcacat
     5041 cgacatcttt cggccgttgc cgccgcgctg gtgtcccgca agacccgccc gatgaaatgc
     5101 ccggtgaggg ggcgtcaacc ggggcacccg cagcgcccgc cctggtggcg gaagcatgcg
     5161 tcgcacgcaa ctcctcgccg gctctcgctg cggacgagga acaggagcca gtgccgccct
     5221 tgcggccgca cccgcaatac gcgaagagca gcagcaagcc tctagtgccg cagttgcccg
     5281 ttgcccgctt cgcggatgac atgccaagga agcgccggct actcgctgaa ctccggtaac
     5341 cggacggtgt cgtctcgttt cgagtccggt tcccctcgcc ggaacgatcg gaatagccac
     5401 tcatcccgat ttgcaggcac ttccgttgtc aattggacaa cgaccgaatg gagctagccg
     5461 gactcgaacc ggcgacctcc tgggtgcgat ccaggcgctc tcccaactga gctatagccc
     5521 cgcgactgca ccggctctca ccggcccggc gaaggatagc cgcgccacgg cggccggagg
     5581 ggcttgtcgg ggcggaccga atcgagccga cctgcctatg taggcttccg ctcgatgggc
     5641 attctcgacg atgccatccg ggagcatctg gagctcaagc gcaggcacgg tgcgtcggag
     5701 gaggagcttc gccagcagga ggtcgaggct ctcggttcgg ctcgccgcga ggctgcggag
     5761 gaggattccg gggagccggc ggccgccgag gcggcgcccg agcccgtgga cgggaacggg
     5821 gcggttccgg ccgcggagga ggccgacctc gagccggcgg agtccgccga gcccgccctg
     5881 gagctcgagc ccgagccctc gcccgagccg gttcaggagc accccgcgcc gccgatcgtg
     5941 gaagagcccg agccggctct cgaagagccg gagccggagc ccgaggagcc ccagcgcgcg
     6001 gacgagcccg agctggtgga ggacccgcgg cgcagcgggg acacgcccac tcgcgggttc
     6061 gaggccctgg aggagcccgg cgaagaggag tccgaagacg aggacgccga cgtgctcgag
     6121 gacacgcccg acttcctcca ggagacgccg gagcacgacc ggctctggtt cgagcagaag
     6181 ccgccgcgcg acttcgattt cgactagcag gcctggacgg ctgttctagg acgcccgtcg
     6241 atcaagggag ttgcggcggg ggtcgcccgc cccggcgacc cccgcactaa tgcaactcag
     6301 cggcgtcccg tgccgctttg ttcgacggaa ccctccatgc cgtcgtgcgt cccttgtcgg
     6361 tcgccacagg ggaaaactga aatccggcgc ccaggcctaa acgcccgttc agctacgcga
     6421 tgtagccccg gacggtcgcg gcctcgacca cccgctcgat gccgacctcg taggccgctt
     6481 cgcggagcgc cgtgccggat tcccgggatc gcgccgagac ctcgcggaat gccttgcgca
     6541 tgatgcgacc aagcctgtcg ttcacctcgc gctcgtccca gcggaagtgc tgcaggttct
     6601 gcacccattc gaagtaagag accaccactc cgcccgcgtt ggccatcacg tcgggcacca
     6661 cgtacacctg cttctcctgg aggatctcat ccgccgcggg cgtcgtggga ctgttcgcgc
     6721 cctccacgac catccgaca
//
LOCUS       DASVEU010000261         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1354599,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000261 DASVEU010000000
VERSION     DASVEU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354599"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..910)
                     /locus_tag="VF225_10985"
     CDS             complement(155..910)
                     /locus_tag="VF225_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7145820.1"
                     /translation="MATVTLGEIPLARIGLGTNRLTNTPENVAFVREAVAADIGLIDT
                     ARVYTGGQSEQTIGATVSSPDACVVATKGGYGSGRPEVLSAEIDESLRRLRTDNIGLY
                     YLHKPDPETPLEESLGVIKEYRDRGAIQHVGVSNVSVEQIERARQVVSIAAVQNQYNL
                     SVRASDDVVDYCAGNGIVFVPYFPLRADGRPAATEIAKRHGATPAQVALAWLLRRSPA
                     TLPIPGTLSLEHLGENLAAAEIELTDDEFEALR"
     gene            complement(916..1779)
                     /locus_tag="VF225_10990"
     CDS             complement(916..1779)
                     /locus_tag="VF225_10990"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX7145821.1"
                     /translation="MVVHPTRPVDEALGALDRWAGRHGVDLVQLEVDGVQREVAAPGK
                     AEPGDLVVALGGDGTVLSALRAAASDAPVLGVACGSLGALSAVSGDELLEALDRFGAG
                     DWTVRSLPALAVRASGAPDDWAVNDFVVVRRGSGQVVAEVAVDGVLYVRLAGDGLAVA
                     TPIGSTAYSMAAGGPVLATNTPAFVCTPVAIHGGSAPPLVVPAGASLTVEVHVRYAGF
                     HVEIDGHRRALEATSFRLTRQENKVELVTFSPSGAALGALRRRGLITDSPRLLMRDDR
                     AQRTGRGGQLP"
     gene            complement(1877..2098)
                     /locus_tag="VF225_10995"
     CDS             complement(1877..2098)
                     /locus_tag="VF225_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145822.1"
                     /translation="MRLETEPAASATFERGWRAALAYVLRREHLRRTVRIALVVGILL
                     TAVNQLDVIVRGDATAITWIKCGTNFVVP"
     gene            complement(2088..2984)
                     /locus_tag="VF225_11000"
     CDS             complement(2088..2984)
                     /locus_tag="VF225_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7145823.1"
                     /translation="MRRSRPNTAFVLSGGASLGALQVGMLRAVYESGIAADVLVGTSA
                     GALNAAFVASRPQSVATANELARVWRGLQREDIFPVSLRTLVGGLCGRRDHLVPDRGL
                     RRLVAQHVEFQDIADAGIPLHVVAFDLNEGCEVLISEGRAPDVIAATAAIPGVFSPVR
                     IGERELIDGGVVNNTPISHAVALGAERIFVFPTQAGQRPLKRAARGALEAGIQGLTLL
                     TDRRLEGDVARYSSDVELILLPAPNPTQVQPIDFDHSSRLIREALTATRAFLARRGTA
                     FAQRAVERAPGTDPPVATRLAA"
     gene            complement(3038..3310)
                     /locus_tag="VF225_11005"
     CDS             complement(3038..3310)
                     /locus_tag="VF225_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145824.1"
                     /translation="MTEQVEKQLVDAAIEAYVDWREECVGVWDAYERWARAPKIDAAG
                     AFSAYRAALDREECASHAYADLLARIARGRRSVGGLFGRTIFALNS"
     gene            complement(3351..4283)
                     /locus_tag="VF225_11010"
     CDS             complement(3351..4283)
                     /locus_tag="VF225_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7145825.1"
                     /translation="MELRHLRYFVAVAEELHFSRAAERLHIAQPAVSEQVRKLEDELG
                     VRLLNRTKRTVSLTDPGATLLVEARRVLQQVEMARLATHEVRDRLANRLRIGYTPASL
                     PTSVSKALQQLAGSLKGLETILEAGFGMELIAAVRDERLDAAIVSLPAPTAGLRTTQL
                     GYERAVATLPATHRHAAQSTIRLARIAPERIVLLPREASRPFYDAVIAACQNAGLSPS
                     LVETPGTSVEPALLAVASGAGIGLLPESVANRYSTPGVRFVSIDGEQPAVATAIVTRR
                     DSAHLPTAAFLRTVSQAHTRRTLAVSATPVPTAA"
     gene            4732..>4994
                     /locus_tag="VF225_11015"
     CDS             4732..>4994
                     /locus_tag="VF225_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145826.1"
                     /translation="MRWEISANLGVHARANMDARGWLWEITRGAQVARVVIEISEAAW
                     SSDPLSLPDDTRQAIETDGRTELLKVLEQDHPPRVIRCGSMGCT"
BASE COUNT          800 a         1678 c         1744 g          772 t
ORIGIN      
        1 ggtgatcctt gccagatcgg acgtgacgag cgtccctcac ccgatcgagg cggagggggc
       61 ctacagactc gaggcgcgtc gtggcaggcg ccactggctt ctcagcaacc cgatctacct
      121 gcgggaagcg gcttcaggcg ctgagcgatg aaggtcagcg gagggcctcg aattcgtcgt
      181 cggtcagctc aatctcggcg gcggccaggt tctcccccaa gtgctcgagc gacagggtgc
      241 cgggaatcgg cagcgtcgcc ggcgatcgcc gcagcagcca ggcgagcgcg acctgcgcgg
      301 gtgttgcccc gtgccgcttg gcgatctctg tcgccgccgg gcggccgtcg gctcgcaacg
      361 gaaagtacgg aacgaacacg atgccgttgc ccgcgcagta gtcgacgacg tcgtcagacg
      421 cgcgcacgga gaggttgtat tggttctgga cggccgcgat cgagacgacc tgtctcgccc
      481 gttcgatctg ctccaccgac acgttggaga cgccgacgtg ctggatggcg ccgcggtccc
      541 tgtactcctt gatgaccccg aggctctcct cgagcggagt ctccgggtcg ggcttgtgta
      601 ggtagtagag accgatgttg tcggtccgca gccggcgaag gctctcgtcg atctcggcgc
      661 tgagcacctc gggtcggccg ctcccgtagc cgcccttcgt cgccacgacg caggcgtcgg
      721 gcgacgagac ggtggcgccg atggtctgct cgctctgtcc gcccgtgtag acgcgagcgg
      781 tgtcgatcag gccgatgtcg gcggcgaccg cctcccggac gaacgcgacg ttctccggcg
      841 tgttcgtgag tcgatttgtc ccaagcccga tccgggcgag cgggatctcg ccgagtgtga
      901 ccgtcgccat gtccgtcacg gtagttggcc tccacggccg gttcgctggg cgcggtcgtc
      961 gcgcatgagc aaccgcggac tgtcggtgat cagtccgcgc cggcgcaagg cgccgagggc
     1021 ggcaccggac ggagagaatg tcacgagctc gaccttgttc tcctgacgcg tcagccggaa
     1081 agaagtggcc tcgagcgcac ggcggtgacc gtcgatctcg acgtggaagc ccgcgtagcg
     1141 gacgtgcacc tcgacggtca agctggcgcc ggccgggacc acgagcggcg gggcgctccc
     1201 gccgtgaatt gccacgggcg tgcagacgaa cgccggtgta ttcgtggcca gcaccggccc
     1261 accggccgcc atcgagtacg cagtcgagcc gataggcgtc gcgaccgcca ggccgtcgcc
     1321 ggccagacgg acatagagca ctccgtcgac tgcgacctcg gcgacgactt gcccggatcc
     1381 gcgacggacg accacgaagt cgttcacggc ccagtcgtct ggagcgcccg aggcacgtac
     1441 ggccagggca ggcaacgatc gcaccgtcca atcgccggcc ccgaagcggt cgagcgcctc
     1501 gaggagctcg tcccccgaca ccgcgctcag cgcgccgagg ctgccgcacg cgacgccgag
     1561 gacgggcgca tcgctcgccg ccgcgcgcaa cgccgagagc accgttccgt cgccgcccag
     1621 cgcaacgaca agatcgccgg gctcggcctt cccgggcgcg gctacctcgc gctgcacgcc
     1681 gtcgacctcg agttgcacga ggtcgacgcc gtgccggccg gcccagcggt cgagcgcccc
     1741 caacgcctcg tccaccggcc gcgtcgggtg aacgaccact gctaggcgcc gcgggctcgt
     1801 ggacgacatc cccgctagtg cggccgctcc gggcgcgcga cggcgcgccc gctgagcagc
     1861 ccaaggttgg agacgatcaa gggaccacga agttcgtgcc gcacttgatc caggtgatcg
     1921 cggtggcgtc gccgcgcacg atcacgtcga gctggttgac ggcagtgagg aggatgccca
     1981 cgacgagcgc gatccgaacg gtccggcgga gatgctcgcg gcgcaggacg taggcgagcg
     2041 cggcgcgcca gccgcgctcg aaagtagcgg atgcggccgg ttccgtctca agccgcaagt
     2101 cgagtcgcga cgggaggatc ggtgcccggg gcgcgctcca cagcccgttg ggcgaacgcg
     2161 gtcccccgcc gggcgaggaa tgcgcgagtg gcggtcagcg cctcccggat caagcggctc
     2221 gaatgatcga agtcgatcgg ctgtacctgc gtggggttag gggcgggcag gagaatcaac
     2281 tcgacgtcgc tcgagtaacg agccacgtcc ccttcgagcc ggcggtcggt cagcagggtt
     2341 aggccctgga tgccggcttc gagcgcacca cgcgccgcac gcttcagcgg ccgctgcccg
     2401 gcctgggtcg ggaagacgaa gatgcgctct gcgccaagcg ccaccgcgtg cgagataggc
     2461 gtgttgttga cgacgccacc gtctatgagc tctcgctcac cgatccgcac gggcgaaaat
     2521 acgcccggga tcgctgccgt cgcggcgatc acgtccggcg cccgtccttc cgagatgagc
     2581 acctcacacc cttcattgag gtcgaaggcg acgacgtgaa gcggaatccc agcatcggcg
     2641 atgtcctgga actcgacgtg ctgagccaca agccggcgca gtcctcggtc ggggaccaga
     2701 tggtcgcgcc gcccgcacag tccgccgacg agcgtgcgca ggctcactgg gaagatgtcc
     2761 tcgcgctgca agccgcgcca aactcgcgcg agctcgttag cggtcgcgac gctttgcggg
     2821 cgcgacgcca cgaaggcagc gttcagcgca cccgcggacg ttcccaccaa tacgtcggcg
     2881 gcaattccgc tctcgtagac cgcgcgcagc atccccacct gtagggcccc caggctggcg
     2941 ccgcccgaaa ggacaaaggc cgtgttggga cggctgcggc gcatggtcga ggccgacctc
     3001 actgcgacac gtcttggtgg cgccgtggcc actcgaatca cgagttgagc gcgaaaatcg
     3061 tccgcccgaa caagccgccg acggagcgtc ttccgcgtgc aatgcgcgcc agcagatcgg
     3121 cataggcatg agaggcgcac tcttctcgat cgagcgcagc acggtaggcc gagaaggcgc
     3181 cggcagcgtc gattttgggg gcgcgcgccc agcgctcgta ggcgtcccaa actccgacgc
     3241 attcctcgcg ccagtcgacg taagcttcga tcgctgcgtc aaccagttgc ttttccacct
     3301 gttctgtcat cacgcttctc cttctggttc cagttggttt cgccagtcga tcaggcggcc
     3361 gttgggacgg gtgtcgctga cacagcaagc gtgcgccgcg tgtgggcctg ggaaacggtc
     3421 cgcaggaagg ccgccgtagg caggtgcgcg ctgtctcggc gcgtcacgat ggccgtcgcg
     3481 acggcgggct gctcgccgtc aatggacacg aaccggacgc cgggagtcga ataacggttg
     3541 gccaccgatt caggaaggag gccgatcccc gctcccgacg cgaccgcaag tagcgccggc
     3601 tcgacgcttg tacctggcgt ctcgacgagc gacggggaca gcccggcgtt ctggcatgcc
     3661 gcaatgacgg cgtcgtagaa ggggcggctg gcctctcgtg ggagcagaac gatgcgctcg
     3721 ggcgcgattc gggcgaggcg gatggtgctc tgcgccgcgt ggcggtgggt cgcaggcaac
     3781 gttgccactg cccgctcgta accgagctgt gtcgtccgta gtcccgcggt cggagcaggc
     3841 agcgagacga tcgcggcgtc gaggcgctcg tcgcggacgg ccgcgatcag ctccatgccg
     3901 aacccggcct ccaggatggt ctccagccct ttaagcgacc cggcgagctg ctgtagcgcc
     3961 ttcgagacgc tggtgggcag agatgccggt gtgtatccaa tccgcagacg gtttgcgagg
     4021 cggtcacgaa cctcgtgggt cgcgaggcgc gccatctcga cctgctgcag cacgcgccgc
     4081 gcctcgacga ggagcgtggc tcctgggtcc gttagtgaaa cggtccgctt ggtccggttg
     4141 agcaggcgca cgcccagctc gtcttcgagc ttgcgaacct gttcgctgac ggccggctgc
     4201 gcaatgtgga gtcgctccgc ggcccggctg aagtgcagct cctctgcgac cgctacgaaa
     4261 tatctgaggt ggcgaagttc catgctcccg atctctgttg gttcatggag cttgcgacaa
     4321 tcgggagctc atcgcatcgg tttagtccct agccgactcc ctagtcgccg ctcgcggccg
     4381 taccaaggtc cctagccgac aggcacggcg acggcgccaa gaggcgctgc ggtcgcaagc
     4441 gccgtatcgg gagggcatcg accggctcca gcgcccgcgc gtccgcgtgc agctcgcccg
     4501 cacccacctg ttatacggcg agtggttgcg gcgcgagcgg cggcggttgg acgcgcgtga
     4561 gcagctgcgc accgcccacg aggtgtttca caagcatggg gcgggggcct tcgcggcgcg
     4621 ggccgagcga gagctgctgg cgagcccctg ctgcgtgctc aaacgcgatg gcctatcagc
     4681 ggtagagggt cacgatcgac cgctgacgct ggcgcgtcgt aatctcgcgt catgaggtgg
     4741 gagatcagcg ccaatctcgg ggttcacgca cgcgcgaaca tggatgcacg cggttggctt
     4801 tgggagatca cccgcggggc ccaggtcgcc cgggtggtca tcgagatcag cgaagcggcc
     4861 tggtccagtg acccacttag tcttccggac gacacgcggc aagcgattga aaccgacggg
     4921 cggacggagc tactcaaggt cctcgagcag gaccatcccc ccagggtcat ccgttgcggc
     4981 tccatgggct gtac
//
LOCUS       DASVEU010000262         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1356914,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000262 DASVEU010000000
VERSION     DASVEU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356914"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF225_11020"
     CDS             complement(<1..368)
                     /locus_tag="VF225_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145827.1"
                     /translation="MRRFAPVGMRARVLFLILLVLLPVFGLRAFSAIDDRRAAIRGAE
                     GDAYDTARIVAQDQQGLLEQSRQQLFTFVQLVALSSGRPIEPRGCKTALVTFMKRLPN
                     YVDLGIVAPNGKVLCAATTKA"
     gene            complement(365..1087)
                     /locus_tag="VF225_11025"
     CDS             complement(365..1087)
                     /locus_tag="VF225_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7145828.1"
                     /translation="MTAVEAGTSDLARAAGLALDAYRRDHVGERIKRALEREGVSDIH
                     SLAALIARDQEARTRFRRSVAVSVSGLFRDPAQFDLLEGTLLPPLIADGRRISVWSAG
                     CADGSELYSVAVLLQRLDVLDRALLLGSDLLDENLVLARRGSYDGGISFSSELRAHIC
                     WERRDLLRAGATPGKWRLILCRNLAIYLAPEPKRALHERLAGALARGGVLLLGRSERI
                     SDPERLGLRRAAPHAYVKEVYA"
     gene            complement(1074..1451)
                     /locus_tag="VF225_11030"
     CDS             complement(1074..1451)
                     /locus_tag="VF225_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145829.1"
                     /translation="MESTILVCDNEEILRGLVRASLDGNGYTLVEARDGDEAVEQARL
                     VRPDLILLDMMMPGQSGLEVLAELRGDPELRGTPVLMLTARAQLTDREAAHLAGADRF
                     LAKPFSPLELDGVVKELLGHDRG"
     gene            complement(1510..2049)
                     /locus_tag="VF225_11035"
     CDS             complement(1510..2049)
                     /locus_tag="VF225_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145830.1"
                     /translation="MAALVFAHAGTAQAAGGNYRFDGGSALERTQVREALKASSFDWS
                     LVKAQVTIHIQHGTVSHAVPGEIWLDADLLDSGRFSWATVQDEYSHQVDFFLFTPEIR
                     AQLQAALGAKAWCYENGSIQAHGDQGCERFTSLLPWAYWQSPDNAYKPTAKTDESAAM
                     APTRFKALLSRLIGTRATR"
     gene            2221..4107
                     /locus_tag="VF225_11040"
     CDS             2221..4107
                     /locus_tag="VF225_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7145831.1"
                     /translation="MAVVIASNLRKEFSGDPLFDGVSFKVERRDRVALAGPNGAGKTT
                     LLRALVGETTLQGGDLAFQKGTRVALHDQRPPLERRLTLREYVLSGARDLIALEEELR
                     RLEQAMAAGDHEDATIRRYGEAQARLEHAGGYDWRERATAVVRGLGFADEQLDRQLDT
                     FSGGELTRASLARALGGDPDLLLLDEPTNHLDVESLEWLERELQSLDAGIMIVAHDRW
                     FLEAVTNVVLELDAGRSTYFKGPWHAWRLEKASRLHAASKAAERVGRDIERLERFVAR
                     FRASTEKMARKAQVKLTQIERLEKERAKSKSEVDLLTRRTRSLGFEFLKPARSGRTVL
                     EVEGLGLRIGDRVLLEAASFAIERGEHVGLVGPNGSGKTTLLETILERRETDDGSSRL
                     GHGVVPAYFSQQEMELDTRGSVLQCVQRATGLQRPDAQKLLGRFLFSGWTEHEKPVVA
                     LSGGERRRLALALVVASGANFLVVDEPTNHLDMESREALEAALEAFPGTILLVSHDRA
                     LLDAVAERTIAIEDRHLNSYDGGWAEYVRQREARQAPAPTPVERKPPAKRRKAPAKPK
                     VPSELERIEAEIAARESTVAELEQRLAADWNDVEMLAAHRHARDELQALLQRWERLFE
                     RAHT"
     gene            complement(4116..4679)
                     /locus_tag="VF225_11045"
     CDS             complement(4116..4679)
                     /locus_tag="VF225_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145832.1"
                     /translation="MRILVAEDETIIRLDLRALLEHAGFEVCAEARDGEEAVALARSE
                     RPDLALLDVKMPRLDGIEAARRILDERPIPIVMLTAYGQDELVQRAAEAGVFGYLVKP
                     FREQDLLPAIRTARARHEELAALREEAESLTEALAARKSIERAKGLLMEKEGLSEQDA
                     FARLRKASQISGRPLKVVAEALIATFE"
     gene            4766..5182
                     /locus_tag="VF225_11050"
     CDS             4766..5182
                     /locus_tag="VF225_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145833.1"
                     /translation="MSDQEFSIPNPLVLRIEISAEQAASLAPLLGGASAIGQDPISAP
                     QRLQPISDYAADARVTELKRLEGDLEQREAEFEADVQFREDRIERWRTELEDLEQTLD
                     RRERDLASYVDQLQGAMSGSDGAWWSRPAGGDGGVR"
     gene            complement(5399..>5581)
                     /locus_tag="VF225_11055"
     CDS             complement(5399..>5581)
                     /locus_tag="VF225_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7145834.1"
                     /translation="DLAQRNGEVVMSIADDGDGIPSDAGGTGLSIVQALVRDELQGRL
                     DLRSEGGTRAEVVFPA"
BASE COUNT          934 a         1905 c         1862 g          880 t
ORIGIN      
        1 gctttcgtgg tcgccgcgca aagaaccttg ccgttcggag cgacgatgcc gaggtcgacg
       61 tagttcggca gacgcttcat gaaggtgacg agggccgtct tacagccgcg aggctcgatc
      121 ggccgcccgg aactgagggc aacgagttgg acgaaggtga atagctgttg tcgcgactgc
      181 tcgagcagcc cttgctgatc ctgcgccacg atgcgcgccg tgtcgtaggc gtcgccctct
      241 gcgccgcgaa tcgcagcccg gcggtcgtcg atcgccgaga aggcgcgcag gccgaagacc
      301 ggcagaagga cgagcaggat gaggaagagc acccgagccc gcatgccgac cggtgcgaag
      361 cgcctcacgc gtacacctcc ttcacgtatg catgtggggc agcacgacgc aggcccaacc
      421 gctcgggatc ggagatgcgc tcgcttcgac cgagcaggag aacgccgccc cgcgcaagcg
      481 ctcctgccag cctctcatgc agtgcacgtt tcggctcggg cgccagatag atggcgagat
      541 tgcggcagag aatgaggcgc cacttcccgg gcgtcgctcc agcgcggagg aggtcgcgac
      601 gctcccaaca gatgtgtgcg cgaagctccg aggagaacga gatcccgccg tcgtaagaac
      661 cgcgtcgcgc aagcacgagg ttctcgtcga gtagatcgct tccgagcagc agcgcccggt
      721 cgagcacgtc gaggcgctgc agcaggaccg caacgctgta cagctcggaa ccgtccgcac
      781 aaccagccga ccagacgctg atccggcgac cgtcggcgat gagtggcggc aagagcgtgc
      841 cttcgagcag atcgaattgg gccggatcgc ggaagagacc cgacaccgaa acggcaaccg
      901 agcggcggaa acgggtacgc gcctcctggt ctcgagcgat cagcgccgcg agtgaatgaa
      961 tgtccgaaac gccttcacgt tcgagcgcgc gcttgattcg ctcgccgacg tgatcacgac
     1021 ggtaggcgtc gagtgccagc ccggccgcgc gcgcgaggtc cgaggtcccg gcttcaaccg
     1081 cggtcatggc ccagcaactc cttcaccacg ccatcgagct cgagcgggct gaacggcttg
     1141 gcgagaaaac ggtcggcgcc ggctagatgg gcggcctctc gatccgtgag ctgggcgcgg
     1201 gccgtgagca tgaggacagg tgtgccgcgc agctcgggat ccccgcgcag ctcggcgaga
     1261 acctcgaggc cgctctggcc cggcatcatc atgtccagca agatcagatc cggccgcacg
     1321 agccgcgcct gctcgaccgc ctcgtcgccg tcgcgagcct cgaccagcgt gtagccgttc
     1381 ccatcgaggc ttgcgcgaac gagccctcgg aggatttctt cgttgtcgca gacgagaatc
     1441 gtggattcca tggccgcgct agaggattcg acaaaagtcg tctccgcctt gaggtccgag
     1501 gcagctggct cagcgagtcg ctctggtgcc gatgaggcgg ctgaggagcg ccttgaacct
     1561 cgtgggcgcc atcgccgccg actcgtcggt cttcgcggtc ggcttgtagg cgttgtcggg
     1621 cgattgccag taggcccagg gtaggagcga ggtgaaccgc tcgcagccct ggtcgccatg
     1681 cgcctggatc gaaccgttct cgtagcacca ggcctttgcg ccgagggccg cctggagctg
     1741 ggctcggatc tcaggcgtga agaggaagaa gtcgacctgg tgcgagtact cgtcctggac
     1801 ggtggcccag gagaagcggc ctgagtccag gaggtcggcg tcgagccaga tctcgcccgg
     1861 aacagcgtgt gagacggtgc cgtgctggat gtggatcgtg acctgagcct tgacgaggct
     1921 ccagtcgaag gagctcgcct tcagagcctc acgcacctgc gtccgctcga gggcgctgcc
     1981 gccgtcgaac cggtaattcc ccccggcggc ctgcgccgta cccgcatgtg cgaagacgag
     2041 cgctgccact gccgccggga ggagagtctg aaggacacga cacatccctt acgttctcgc
     2101 ccacgttcag ccgcccgctg tcccctgttt tccgcaacgg cgaccgaatt tgtcccccgt
     2161 ctggcgcgcc ttcctgccgg ccgtcccccg tccgcgccac gctgactagc ctgctaggag
     2221 atggcagtcg taatcgcatc caacctgcgc aaggagtttt ccggcgaccc gctgttcgac
     2281 ggcgtttcgt tcaaggtcga gcgccgcgac cgcgtcgcgc tcgccgggcc caacggcgcc
     2341 ggcaagacga ccttgcttcg agcgctggtt ggcgagacga ccctccaggg aggcgacctg
     2401 gcattccaga agggcacgcg cgtggcgctg cacgaccagc ggccgccgct cgagcgccgc
     2461 ctgacgctgc gcgagtacgt cctgtccggg gctcgcgacc tgatcgcgct cgaggaagag
     2521 ctgagacggc tcgagcaggc gatggctgct ggagaccacg aggacgccac gattcgccgc
     2581 tacggcgagg cccaggcgag gctcgagcac gccggcggct acgactggcg tgagcgcgcg
     2641 accgccgtcg tgcgcgggct cggcttcgcg gacgagcagc tcgaccgaca gctcgacacc
     2701 ttctccgggg gcgagctcac gcgtgcctcg ctggcgcggg cgctcggagg cgatccggat
     2761 ctcctgctcc tcgacgagcc gaccaaccat ctcgacgtcg aaagcctgga atggctggag
     2821 cgcgagctgc agtcactcga cgccgggatc atgatcgtcg cgcacgaccg ctggttcctc
     2881 gaagcggtga cgaacgtggt cctcgagctc gatgcaggtc gctcgacgta cttcaaggga
     2941 ccgtggcacg cgtggcgcct cgagaaggcc tcccgcctcc acgccgcctc gaaggcggcc
     3001 gagcgtgtcg gccgggacat cgaacggctg gaacgcttcg tcgcacgctt ccgggcctcc
     3061 acggaaaaga tggcacgcaa ggcgcaggtg aagctgacac agatcgagcg cctggaaaag
     3121 gaacgcgcga agtcgaagag cgaagtcgat ctgctgacgc ggagaaccag gagcctcggc
     3181 ttcgagttcc tcaagccggc gcgcagcggc cgaaccgtcc tcgaggtcga agggctcggc
     3241 ctccggatcg gcgaccgcgt gctcctggaa gccgcgagct tcgcgatcga gcgcggcgag
     3301 cacgtggggc tcgtcggccc gaacggatcg ggaaagacca cgctgttgga aaccatcctg
     3361 gagcggcgcg agaccgacga cggctcctcg cgcctggggc acggtgtcgt gccggcgtac
     3421 ttctcccagc aggagatgga gctcgacacc cgtggatccg tcctgcagtg cgtccagcgc
     3481 gcgacggggc tgcagcgtcc cgacgcgcag aagctgctcg gccgtttcct cttctccggc
     3541 tggacggagc acgagaagcc cgtcgtcgcg ctctcaggcg gtgagcgccg gcgcctcgcc
     3601 ctggcgctgg tcgtcgcctc cggcgcgaac ttcctcgtcg tcgacgagcc gacgaaccat
     3661 ctcgacatgg agagccgcga ggcgctcgag gcggcgctcg aggctttccc cgggacgatc
     3721 ctgctcgtct cacacgatcg tgccctcctc gacgcggtgg ccgaacgaac gatcgcgatc
     3781 gaggaccggc acctcaattc ctacgatggc ggctgggcgg aatacgttcg ccagcgtgaa
     3841 gcgcgccaag ctccagctcc tacgccggtc gagcgcaagc ctccggccaa gcggcgcaag
     3901 gcaccggcca agccgaaggt tccctcggag ctcgagcgga tcgaagccga gatcgccgcc
     3961 cgcgagagca ctgtcgcgga gctcgaacag cgcctggccg cggactggaa cgacgtcgag
     4021 atgctcgccg ctcaccgcca tgcccgtgac gagctccagg ctctgctcca gaggtgggaa
     4081 cgcctcttcg agcgagcgca cacctgagct caggcctatt cgaaggtcgc gatcagcgct
     4141 tccgcaacga ctttgagtgg gcgcccggaa atctggctcg ccttgcgcag ccgcgcgaac
     4201 gcgtcctgct ccgagaggcc ttccttttcc atcagcaggc ccttggcgcg ctcgatcgac
     4261 ttccgcgcgg caagcgcctc ggtcagtgac tccgcctcct cccgcagagc ggcgagctct
     4321 tcgtgccggg cgcgggccgt gcggatggca ggcagcagat cctgctcacg gaaaggcttc
     4381 acgaggtagc cgaacacacc cgcctcggcg gcgcgctgga cgagctcgtc ctgcccgtac
     4441 gcggtcagca tgacgatcgg aatcggccgc tcgtcgagga tccgccgggc cgcctcgatc
     4501 ccgtcgagcc tcggcatctt gacgtccaga agggccagat ccggccgctc cgagcgggcc
     4561 agggccacgg cttcctcgcc gtcccgggcc tccgcgcaca cctcgaaacc ggcgtgctcc
     4621 agcagggccc ggaggtcgag cctgatgatc gtctcgtcct ccgcgaccag aatcctcacc
     4681 tgtggtgggt gagtttggcg gaaaccgctc agccgaggga tagtgccgcg cgcctccccg
     4741 ggcgattcag gtggtgcggc tcctcatgtc tgaccaggaa ttcagcatcc ccaaccctct
     4801 cgtgctccgc attgaaatct cggcggagca ggctgcctcc ctggcgcctc tcctcggtgg
     4861 cgcaagtgcc atcgggcagg acccgatttc cgccccgcaa cgtttgcagc cgatttccga
     4921 ttacgcggcc gacgcccggg tcaccgagct caaacgcctc gagggcgacc tggaacagcg
     4981 ggaagccgag ttcgaggctg acgtccagtt ccgcgaagac cggatcgagc gctggcgcac
     5041 cgagctcgaa gacctcgagc agaccctcga ccgtcgcgag cgagatctcg ccagctacgt
     5101 cgaccagctc cagggcgcaa tgtccggcag tgacggcgcc tggtggtcac gccccgccgg
     5161 cggcgacggc ggcgttcgtt aacccgagcg aaagcttcgc tttcgctcgg atcttgggta
     5221 cggcgcttcg cgcgtaacgt gcacgggcca agcccgcgca ctcctttggt gttagtgctt
     5281 agcgattgtt cccttccgtg cgcgcacggg cgctgaagcg tccgcgcccg catcagtaca
     5341 cctccagcta agcccggctt cgcctggtct tagcttccgg ccttcgtgtt tcgctgcgtc
     5401 aggctggaaa aaccacttcg gctctcgttc ctccttcgct acggaggtcg aggcggccct
     5461 gcagctcatc tcgcactagc gcctggacga ttgagagtcc cgtgccgccg gcgtcggacg
     5521 ggattccatc gccgtcgtcg gcgatggaca tcactacctc gccgtttctc tgggcgagat
     5581 c
//
LOCUS       DASVEU010000263        14413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_968831,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000263 DASVEU010000000
VERSION     DASVEU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968831"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..871)
                     /locus_tag="VF225_11060"
     CDS             complement(158..871)
                     /locus_tag="VF225_11060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX7145835.1"
                     /translation="MEVLVLQHIACEPPGAYEDVLNERGATIHRVELDEGDPLPDWRT
                     FDAIVAMGGPMGALDDDEHPWLVDEKRLIGDAVRSGLPFWGVCLGVQLLAASLGARVY
                     PGSAPEVGLLPVMLTDEALADPVFAGMPGELLSLQWHGDTFDLPDGAARLAGSPAYPN
                     QAFRVGAAAYGVQFHLEVSPGLAREWADVPAYAESLERVLGPGALERLLAELEANADG
                     VLSDGRRLFERWLDAATRA"
     gene            complement(872..1534)
                     /locus_tag="VF225_11065"
     CDS             complement(872..1534)
                     /locus_tag="VF225_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145836.1"
                     /translation="MTIRSKLYAAIVVTVAGLALTAGAGIWGMNRLSDRFDGVQRADA
                     ARALALQLKFGVADFNGWQTAYGYDNGKSRPIFLSSVAGFRQTLARAKRELRRPIERT
                     LLAEISVAFGDFMRLDEVAWAALQAGRTAEVRRLFLGPEIANFRRAAAGAQGLAQVEE
                     KRANAEERRFREARKDALRALIGASLVAALLVAILLVTALDLARMAERALAQSSSPAE
                     VE"
     gene            complement(1531..2808)
                     /locus_tag="VF225_11070"
     CDS             complement(1531..2808)
                     /locus_tag="VF225_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7145837.1"
                     /translation="MTVKDVILTVTLMLGAGLCARLVADLLHLPHMLVLLGTGILLGS
                     SVSDAIDVPLDSMGSQLVLTLGVSFILFHGGLQLSLGILRKVAVGLLLLVVPGVVLTA
                     LVTGSVAALLFGLPLSSGLLIGAALSPTDPAILVPLFERLRVRPKVSQTIIAESALND
                     PTGAVLALAFAGVVLSGRASLTAPMVDFVKDLAISTAMGVVFGIVLSAAVSSRRAGIW
                     RESCAIAVLAVVAGSFFSIDSAGGSGYLGAFLAGLIVGNMDRLGLAMFSHHEHDMRVL
                     ISTVADVMVIFVFITLGANLPWTTIADHFAPALGVLAALILLARPLAVLVCLLPDRRA
                     AWTRQELFFLAWTRETGVVPAAIAGIIVSMHVPNSDLVVTTVAMAIVLTLALQATTKG
                     WLARRLDLLEPDARVATPPGPGVPATAPTAASP"
     gene            2886..3098
                     /locus_tag="VF225_11075"
     CDS             2886..3098
                     /locus_tag="VF225_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145838.1"
                     /translation="MLIFLLLGVYPWVLLLTFAISLYLTFIETREQRMDLRHTAWWLQ
                     LTFLFHFAGYLALRGWTFYRRRHSPA"
     gene            3121..3423
                     /locus_tag="VF225_11080"
     CDS             3121..3423
                     /locus_tag="VF225_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145839.1"
                     /translation="MTWSRVAFMVALLALGLVLTRVLGRTNPKFSKQDAIAAARPNVD
                     FKPQGYNIRLLRQGIPPRPVWAVSFWIRKAEGGYSKITLVLVDANSGRVMQVRRTT"
     gene            3608..5074
                     /locus_tag="VF225_11085"
     CDS             3608..5074
                     /locus_tag="VF225_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145840.1"
                     /translation="MPTPQELSDKLTLDSQILAEQFYYFTVVIMWLIHVGFMSYEAGA
                     GRRKNVMSTAMKNILTIAVVTPTFYYFGWWVYGCFNTGIIPFTPSKFVHSVHASEVAM
                     ANFCSTTYPWAANFGPNLQDHISGVFWAAFLLFSWTTASIVSGALIERVRLSAYLILA
                     CILGSFIWILDASWGWSAYGFLNMRWGFHDSIAGLVVHGVAGAFTLGVLLNLGPRIGK
                     YTRQGLARTFRPHNLHLTLMGLMLIFTGFYAFYAACLVIQSTSLPGWGSIYLNPTTLS
                     AITYAITIGFAGGFTGGYFASRGDPFWTLSGGLAGVISVSAGADVYAPSLVYLIAIFG
                     GALAVYAGNFIEKKLRIDDAVGAVAVHGVCGFWSPLAVGLFAAGYPTGLNNIDSSLTG
                     QLYGMMIFLPLGFFGGYIPSWILKKFNLLRVPPEVELEGLDMAEFNADFYPEFERPAE
                     VIIEPDGTQVPSAPILLEDYANVIQNGNGRAREPSVRG"
     gene            5198..5542
                     /locus_tag="VF225_11090"
     CDS             5198..5542
                     /locus_tag="VF225_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145841.1"
                     /translation="MALGWGTDPSCIQHGVPPADGVYKPFEKCADFFTFGPGSKPGTL
                     FDGVSNGYLALTAIGFVVMLGFLVYWVILEDGKLKRQAAHLLVAGRAGQVQLSPEVRA
                     DVPQPGLGGPAE"
     gene            5561..5722
                     /locus_tag="VF225_11095"
     CDS             5561..5722
                     /locus_tag="VF225_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145842.1"
                     /translation="MADRVKRTQQIVFAPMQRHSVRFEQVFIVVCILCIILGVVIAKA
                     TDWGVTTRP"
     gene            complement(5818..6594)
                     /locus_tag="VF225_11100"
     CDS             complement(5818..6594)
                     /locus_tag="VF225_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7145843.1"
                     /translation="MSEQRVLGEGLSTVLLLHAGIADSGMWRPQIEALQAAGFRVLAP
                     DLRGFGQRALEPAPFSYVRDVEVLLDGPTTVVGNSLGGRVALELALYRPELVERLVVI
                     APGLPGWEWSPETRAGWTDEETAYDSGDLDGAAEASLRMWVDGPQRAPEEIDPAIRSA
                     VAEMVLRSYEMQAHGWEEGAQEEDVLDPPVNLRLHEIRCPTLVIVGENDVADMRSIAA
                     HVANSVDSARLVEVAGAAHLPSLEKPDEVNALLFAFLIDR"
     gene            complement(6755..7033)
                     /locus_tag="VF225_11105"
     CDS             complement(6755..7033)
                     /locus_tag="VF225_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145844.1"
                     /translation="MATARFVQTGITPDEYDQMREKLGVGDSPPAGGVFHAAAVGEDG
                     KIRVFEVWDSREQAEAWGEKVMAVRTEAGFGDGPPSIEYLDVHKVVQR"
     gene            complement(7342..8997)
                     /locus_tag="VF225_11110"
     CDS             complement(7342..8997)
                     /locus_tag="VF225_11110"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX7145845.1"
                     /translation="MLSSLEIAQQATLRPIAEIAADAGLEPDEIEFYGSYKAKVNLSA
                     LDKRASVADGKLVCVTAITPTRAGEGKTTTSVSLTQGLGAVGAKPVLCLREPSLGPVF
                     GIKGGAAGGGHTQVVPMEDLNLHFTGDMHAIGVANNLLATILEAHILHGNQAGVDPLS
                     VTWRRCMDMNDRALRDIVVGLGGKANGYVRQTGFDITAASEVMGIVAVSRDLADLRAR
                     LGAITVAQSYEGEPVTAEQLRAAGAMTVLLKDALKPNLVQTLEGQPAFIHCGPFANIA
                     HGNNSLVADRVALKLGDYVVTESGFGSDMGMEKFLNIVCRAGGLSPSAIVLVATARAL
                     KHHGGDPDGGLDAIERGAANLARHLGIIAGFGLKAVVAVNRFPGDTDEEVELVRRLAV
                     EHGAHAAEANEGFERGGAGATALAEAVIDAAAHPNSFEHTYPLDAPIADKVQAIARRV
                     YGADNVHFLPVAEQKIARFAEQGLGELPICMAKTHLSLSDDPTLLNAPTGFTVHVRDV
                     RAYTGAGWLVPLCGEIMTMPGLGRKAAAFDVDIDEHGRTVGLF"
     gene            complement(8998..10212)
                     /locus_tag="VF225_11115"
     CDS             complement(8998..10212)
                     /locus_tag="VF225_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7145846.1"
                     /translation="MRDIFRVERMWRPRRELKESYDVVIIGAGSHGLATAYYLAKNHG
                     ITDVAILEQSYIGSGAAGRNTTIIRANYRTPEGAAFYRRSVELYEGLGAELDFNLLFS
                     QQGHLTLAHSDRAMITMAERAEVNQLLGIDSRLVDAEEVGRLCPELNLSSDVTWPIMG
                     ALYHAPGGIIRHDAVVWGLARAADRRGVEIHPYTKVTGIERSNGRVTGVRTNRGDVEA
                     GTVINATAGWSTLVADLAGVPLPITTHILQAFVTEPLKPFLDVIIVSSQMHVYISQTD
                     RGEFLIGAEIEPYTTYLGTSTFPFLEYSARHTLELFPQLESAKILRQWTGLCDLSPDY
                     SPILGVTEVDGFLISCGWGTYGFKAAPIVGTTLAELVATGRTPQLIAPFALERFRTDT
                     LVSELAAAAVSH"
     gene            complement(10209..10694)
                     /locus_tag="VF225_11120"
     CDS             complement(10209..10694)
                     /locus_tag="VF225_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145847.1"
                     /translation="MTELAFLSPDRARTEDLFAPRFASPLARVLAGTTAVRDLSQLGK
                     IEVRADVDAIDVDAEVVRITSRRALVLCPYEQCAELRARLPGMVIDMTGALGGLELEG
                     ERAMRRLTDLDLEALPAAGKVAEVSALVLRDGERFRIFFPQEFAHYVGEVVLDVLEGI
                     E"
     gene            complement(10691..13288)
                     /locus_tag="VF225_11125"
     CDS             complement(10691..13288)
                     /locus_tag="VF225_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7145848.1"
                     /translation="MRLPQQPNERIDRARPIRFFFGGRPVEGFEGDTIGSALFASGRR
                     VFSRSFKYHRRRGLLCCSGACPNCMMQVDGDPNVRVCVEPVREGAQVHAQNVLGSLER
                     DLLAAVDHFGGPFTPVGFYYRTMIRPRGMWPVYEKFLRNIAGLGRLDKHAGRSRRFDT
                     EHRRAEVLVIGGGRAGREAARSAAAEGRQVVLVDERGVPGANGDFEVLAPARAIGIYE
                     GGLVPVDAGSVLYRFRAERIVVATGALEQPLVFPGNDLVGVYLPEAARRLVDEWSLKP
                     GERAVIVSADDRGLAVADQLQRAGTSVVESIDLRSAPPRQLAARGRRGRLTSVVVDGR
                     EVQCDMLVASGGRQPAYSLLAQAGARIEYNAGRGIFVPSDLPANVEAVGSVAGELAPA
                     AVPAASFNGADEAGRCFVCVCEDVTEKDMKRAIAEGFDSIELAKRYTTVTMGPCQGRL
                     CHVPAIRLFAREQETDEATIGTTTARPPWAPVSLGLLAGRGHEPTKRTSIHHRHEEAG
                     ATMMWTGLWRRAHSYEHAGNEALNVHRALGVIDVSTLGKLLVQGPDAARFLELLYPNR
                     FGDMKVGRIRYGVLGTDAGRIMDDGTIARLGDEQYYVTTTSTGADTVIEWFEWWNAIW
                     RLDVETVNLTGSLAAVNVAGPRSRQLLGRLADVDTSNDGISYLDAKETLVAGVPCLIL
                     RIGFVGELGYELHFASPYGEYMWDTLLEYGADLGIRPFGLEPQRILRLEKMHILIGQD
                     TDSESNVLEAGMPWIAKLDKDDFVGKWALEHVQERGFREQLVGFEMTDGVVPAEGGQI
                     VLGGRSAGRVTSARLSPQLGRAIGMAWVRPELAQEDTEIEIRVNGGVASARVRLKPFF
                     DPEGAQLRS"
     gene            complement(13285..13563)
                     /locus_tag="VF225_11130"
     CDS             complement(13285..13563)
                     /locus_tag="VF225_11130"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX7145849.1"
                     /translation="MSFLLPCPNCGPRDVNEFAYAGEVTHRPRTTPSQQELSAYVYFR
                     RNVAGVQREWWYHRFGCELWFLADRDTRTNEVLHTELPQASAETPAAR"
     gene            complement(13572..>14413)
                     /locus_tag="VF225_11135"
     CDS             complement(13572..>14413)
                     /locus_tag="VF225_11135"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX7145850.1"
                     /translation="GQGAKPGGGGMLLGHKISDRVAAMRSLPQGIDQRSASRHPDWTG
                     PDDLEIKIHELREITDWEKPIFVKIGATRTHYDVQLAVKAGADVIVVDGMQGGTAATQ
                     DVFIEHVGIPTLPATRLAVHALQELDMHRKVQLIISGGIRTGADVAKALALGADAVSI
                     GTAALISLGDNSPEYEEEYRKLGSAAGFYDDWQAGRDPAGISTQDDELSARLDPVLGG
                     RRIANYLRVLTLEAQTLARACGKSHVHNLEPEDLVALTVEAAAMARVPLAGTDWIPGR
                     HSA"
BASE COUNT         2346 a         4904 c         4797 g         2366 t
ORIGIN      
        1 gatcgtttcg ggcttgatcg agtgtcggga acggatcggg aatgggtccc ttcccgagga
       61 ctcgctgaac ctcggtcgga cctgattcga cgactgccat ggccaactcc ttcttcgggc
      121 gaggtagggc ggtgtctaga gcggaaccgt ggcgatgtca agcccgcgtc gcggcatcga
      181 gccagcgctc gaacaggcgc cgcccgtccg agagaacccc gtcggcgttc gcctcgagct
      241 cggcgagcag acgctcgagc gctcccgggc cgagcacacg ctcgagcgat tcggcatacg
      301 cgggcacatc ggcccactcg cgtgcgaggc cgggcgagac ctcgaggtga aactggacgc
      361 cgtaagccgc ggccccgacg cggaacgcct gattcggata cgccggcgac cccgccaggc
      421 gcgccgcccc gtcgggtagg tcgaaggtgt cgccgtgcca ctggagactc agcagctcac
      481 cgggcattcc ggcgaaaacg gggtcggcga gggcctcgtc tgtgagcatc acgggtagca
      541 ggccgacctc gggcgccgac cccgggtaga cgcgggcgcc gagactggcg gccaacagct
      601 gaaccccgag gcagacgccc cagaagggga gcccggagcg cacagcgtcc ccgatcaacc
      661 gcttctcgtc cacgagccag ggatgctcgt cgtcgtcgag cgctcccatc ggcccgccca
      721 tcgcgacgat cgcgtcgaag gtacgccagt ccgggagcgg atcgccctcg tccaactcga
      781 cgcggtggat cgtcgcgcca cgctcgttca agacgtcttc gtacgcgccg ggcggttcgc
      841 acgcgatgtg ctggaggacg agaacttcca cctactcgac ctctgcgggg gaggaagact
      901 gtgccagcgc tcgctcggcc atccgggcaa ggtccaaggc ggtgacgagc aggatcgcga
      961 cgaggagcgc ggccacgagg ctcgccccga tcagggcgcg cagcgcgtcc ttgcgcgcct
     1021 cacggaacct tcgctcctcg gcgtttgcgc gtttttcttc gacttgcgca aggccctggg
     1081 caccggccgc agcgcgcctg aagttggcga tctcgggacc caggaagagc cggcgtacct
     1141 cggcggtccg cccggcctgg agagcggccc aggccacctc gtcgaggcgc atgaagtctc
     1201 cgaacgcaac gctgatctcc gcgagcagcg tgcgctcgat cgggcgcctg agctcgcgct
     1261 ttgcccgggc gagcgtttgc cggaagccgg cgaccgagct caggaagatc ggccggctct
     1321 tgccgttgtc gtagccgtag gcggtctgcc agccgttgaa gtcggccact ccgaacttca
     1381 actgcagggc gagcgcccgc gcggcgtcgg cgcgctggac gccgtcgaac cggtcgctga
     1441 gacggttcat cccccagatg ccggctcccg cggtgagagc gaggccggcc accgtaacca
     1501 cgatcgcggc gtagagcttc gacctgatcg tcatggcgag gccgccgtag gagctgtcgc
     1561 cgggacccca ggccctggag gcgtcgcgac gcgcgcgtcc ggctcgagca gatccaggcg
     1621 tcgcgcgagc cagcccttcg tggtggcctg cagcgcgagc gtgagcacga tcgccatagc
     1681 gaccgtggtg acgacgaggt ccgaattcgg aacgtgcatg ctgacgatta ttccggcgat
     1741 tgctgcgggc acgacaccgg tctccctcgt ccacgcgagg aagaagagct cctggcgcgt
     1801 ccacgcggcg cgtcggtccg gcagcaggca gacgagcact gcgagcggac gggcgagcag
     1861 gatcagcgcg gcgaggactc ccagagcagg agcgaagtgg tcggcgatcg tggtccaggg
     1921 aaggttcgcg ccgagcgtga tgaagacgaa gatcaccatc acgtcggcca cagtcgagat
     1981 gagcacgcgc atgtcgtgct cgtggtgaga gaacatcgcc agaccgagcc ggtccatgtt
     2041 cccgacgatc agcccggcca ggaacgcgcc gaggtagccg cttccgcctg ccgagtcgat
     2101 cgagaagaag ctccctgcga ccacggccag cactgcgatc gcgcacgatt cacgccagat
     2161 tcccgcgcgc cggctcgaaa cggcggcgga gagaacgatg ccgaagacga cgcccatcgc
     2221 tgtcgagatc gcgaggtcct tgacgaaatc gaccatcggt gcggtcagcg aggcacgccc
     2281 actcagaacg actcccgcga aggccagagc tagcacggcg cccgtcggat cgttcagcgc
     2341 agactcggca atgatcgtct gcgagacctt cggccgaacg cgtaaccgtt cgaagagcgg
     2401 gaccaggatg gcgggatcgg tcggtgagag cgctgcgccg atcaggagac cggaggacaa
     2461 aggcaagccg aacagcaacg cggcgaccga gccggtgaca agtgcggtca gcacgacgcc
     2521 gggcacgacg agcagcagga gcccgacggc gaccttcctc aggatcccga gggaaagctg
     2581 gaggccgccg tgaaagagga tgaaggaaac gccgagcgtc agcacgagct gcgagcccat
     2641 cgagtcgagc ggcacgtcga tcgcgtcgga aaccgatgag cccaagagga tccccgtccc
     2701 cagcagcacg agcatgtgcg gcaggtggag caagtccgcg acgagccgcg cgcagagccc
     2761 ggctccgagc atcagtgtca ccgtgaggat gacgtccttg actgtcatga gggggcgatt
     2821 ctcccccggg cgaggcgact cgtccacctt gcgcttgccg ccgccgtgtc ggacactacg
     2881 cgcgcgtgct catctttcta ctcctcggcg tctatccatg ggttcttctg ctcacgttcg
     2941 cgatctcgct ctacctcacc ttcatcgaga cacgagaaca gcggatggac ttgcgtcaca
     3001 cggcctggtg gctgcagctc acctttctct tccatttcgc cggctacctc gccctgagag
     3061 ggtggacctt ctatcgccgc cgtcactccc cggcctagtc tgagcgcccg cctcgcgcgg
     3121 gtgacctgga gccgcgtcgc cttcatggtc gcgctgctcg cgctcggact ggtgttgact
     3181 cgcgtcctcg gccggacgaa ccccaagttc agcaagcagg atgcgatagc cgccgcgcgg
     3241 cccaacgtcg atttcaagcc gcagggctac aacatccggc tcctgcgcca ggggattcct
     3301 cccaggcccg tctgggctgt ctcgttctgg atccggaagg ccgaaggcgg ctactcgaag
     3361 atcaccctcg tgctggtgga cgcgaacagc ggccgagtca tgcaagtacg tcgcacgacc
     3421 tgagtagcgc cagtgttcgc tctgcacacc caccagtggg gaaagctgtg cggcccctac
     3481 gttgtgcacg cggccgcccg ttcttacgct cgcctgtgct cgtaagggtt tctcgcttta
     3541 tcgtcgagag aggcaggtca ccatcgtcgc gtcgctgatt gccggactgg gcgctttggt
     3601 cgctcaggtg ccgacgcccc aagagctgtc ggacaagttg accctcgaca gccagatcct
     3661 ggccgagcag ttctactact tcaccgtcgt gatcatgtgg ctgatccacg tcggcttcat
     3721 gtcgtacgag gccggcgcag gtcgacgcaa gaacgtcatg tcgaccgcga tgaagaacat
     3781 cctcacgatc gcggtcgtga cgccgacgtt ctactacttc ggctggtggg tctacggctg
     3841 cttcaacacc ggaatcatcc cgttcacgcc gagcaagttc gtgcactcgg tgcacgcgag
     3901 cgaagttgcg atggcgaact tctgctcgac gacgtatccg tgggccgcca acttcgggcc
     3961 taacctccag gaccacatct cgggcgtttt ctgggcagcc ttcctgctgt tctcgtggac
     4021 gaccgcatcg atcgtgtcgg gcgcgctgat cgagcgggtg cgcctgtcgg cgtacctcat
     4081 cctcgcctgc atcctcggat cgttcatctg gatcctcgat gcgtcgtggg gctggagcgc
     4141 ttacgggttc ctcaacatgc gctggggttt ccacgattcg atcgccggcc tggtcgtgca
     4201 cggggtcgcc ggagcgttca cgctcggcgt gctgctgaac ctgggcccac ggatcggcaa
     4261 gtacacgcgt caaggcttag ccaggacctt ccggccgcac aacctccatc tgacgttgat
     4321 gggcctgatg ctcatcttca ccgggttcta cgcgttctac gcggcgtgcc tcgtcatcca
     4381 gtcgacctcg ctgcccggct ggggatcgat ctacctcaac ccgaccacgc tctcggcgat
     4441 cacgtacgcg atcacgatcg ggttcgcagg cgggtttaca ggcggctact tcgccagtcg
     4501 gggcgatccg ttctggacgc tgtcgggcgg ccttgccggc gtcatctccg tctcggccgg
     4561 agcggacgtc tacgcgccct cgctcgtcta tctgatcgcg atcttcggcg gcgccttggc
     4621 cgtctatgcc ggcaacttca tcgagaagaa gctgcgcatc gacgacgcgg tgggcgcggt
     4681 cgccgtccat ggagtctgcg gcttctggag cccgctggcc gtcggcctct tcgcggccgg
     4741 ctatccgacc ggcctgaaca acatcgattc gtcgctgacc ggacagctct acggaatgat
     4801 gatcttcttg ccgctcggct tcttcggcgg ttacataccg tcatggatcc tcaagaagtt
     4861 caacctgctc cgcgtgccgc ctgaggtcga gctggaaggc ctcgacatgg ccgagttcaa
     4921 cgccgatttc tatcccgagt tcgagcgacc ggcggaggtg atcatcgagc cggacggcac
     4981 gcaggtcccg tcggctccga tcctgctcga ggactacgcc aacgtgatcc agaacggaaa
     5041 cggacgcgcg cgcgaaccct cggtgcgagg ctgacccgtg gtggagctcg tcttcagcat
     5101 cctgctcatg atcgccgtcg tcgggagtct cggcggctgg gcatatcgca aggaccggcg
     5161 tatgcgtcgg gccagagaga actagggagg gagcgaagtg gcactcggct ggggtactga
     5221 tccttcttgc atccagcatg gggttccgcc tgccgacggc gtttacaagc cgttcgagaa
     5281 gtgtgccgac ttcttcacct tcggcccggg ctcgaagccc gggacgctgt tcgacggcgt
     5341 gagcaacggc tacctcgcgc tcactgcgat cggcttcgtc gtcatgctcg gcttcctcgt
     5401 ctactgggtg atcctggagg acggcaagct gaagcgacaa gcagcccacc tgctggttgc
     5461 ggggcgggca gggcaggtgc agctcagccc cgaggtccgc gctgacgttc cccagcccgg
     5521 gctcggcgga ccggcagagt aggacgagga ggtagacgag atggcggacc gagtcaaacg
     5581 cacgcagcag atcgtgttcg cgccgatgca acgccactcg gtcaggttcg agcaggtgtt
     5641 catcgtcgtc tgcatcctct gcatcatcct cggcgtcgtg atcgcgaagg cgaccgattg
     5701 gggcgtcacg acacggccct gagagcctga gggctcacac gccttcctga gccggcgccg
     5761 gtcccaatcg cgggggctcg gctcaggcga cagggcgttc gcgcatgcgg gtgtcctcta
     5821 gcggtcgatg aggaacgcga acaggagtgc gttgacctcg tcgggttttt cgaggctcgg
     5881 caagtgggcc gcgcccgcaa cctcgaccag tcgagcactg tcgaccgagt tagcgacgtg
     5941 ggcggcgatc gagcgcatgt cggcgacgtc gttttcgccg acgatcacga gcgtcgggca
     6001 gcggatctcg tggagccgaa gattgacggg cggatcgagg acgtcctctt cttgtgcgcc
     6061 ctcctcccat ccgtgtgcct gcatctcgta ggagcgcagc accatctcag ccaccgccga
     6121 ccggatcgcc ggatcgatct cctccggcgc gcgttgaggg ccatcgaccc acatccgcag
     6181 gctcgcttcg gccgcgccgt cgagatcacc cgagtcgtag gctgtctcct cgtccgtcca
     6241 acccgcacga gtctcgggag accactccca accgggcagg ccaggcgcaa tgacgacaag
     6301 ccgttccacg agttctggac ggtagagcgc taactcgagc gcaacgcgac cgccgagcga
     6361 gttgccgaca acagtggtgg ggccgtccag cagtacctct acgtctcgga catatgagaa
     6421 cggcgccggt tccaacgcgc gctgtccgaa cccgcgcagg tcgggcgcaa gaacgcgaaa
     6481 accagccgcc tgcaaggctt cgatctgcgg ccgccacata ccactgtccg cgatcccagc
     6541 gtgcagcaat agaacggtgc tgagaccctc gccgagaacc cgttgctcac tcactgcacg
     6601 agctaaccac gccaataccg cccccacaag agcgacgacc cctcatgtaa tcccgaacac
     6661 tggcgcagcc gacgatcact ccggaacgct gacgcagacg ataggcccgg cgcgagcgac
     6721 ctgagtcgct cgcgccgctt ttgtcccgaa ctgactagcg ctgaaccacc ttgtggacat
     6781 cgaggtattc gatcgagggt ggcccatctc cgaagcccgc ctccgtgcgg accgccatca
     6841 ccttctcgcc ccacgcctca gcctgttcac gcgagtccca tacctcaaag actcgaatct
     6901 ttccgtcttc gccgaccgcg gccgcgtgaa acacgccccc agccggtggg ctgtcgccga
     6961 caccgagctt ctcgcgcatc tggtcgtact cgtcgggtgt gatccccgtc tggacgaacc
     7021 ttgctgtcgc cacaccaacc tcctcgctag acgcctcgac attactccga tgcggcgcgt
     7081 caaactcgcc ccgctcaggc gtcattgccg aacgctcgcg caggcggcga tcctctatcc
     7141 agacccgggc gcgactggac gcgagagccc acccggcgga tgatcgtgtc gtgcgaagcc
     7201 cccgtcggct cgagctcgag gcagtactgg cccggcccct catcgatgct ctcggcgagg
     7261 acgcgcgtac gcgcgcgagc tgtcgatcgg ccctgcgctg ggtcgcgctg aggcgattac
     7321 cttggcccaa tcgctcctgg tctagaaaag gccgacagtg cgcccgtgct cgtcgatgtc
     7381 gacgtcgaag gcggcggcct ttctcccgag ccccggcatc gtcatgatct cgccgcagag
     7441 cggcacgagc cacccggcgc ccgtataagc gcgcacgtcg cggacgtgca ccgtgaatcc
     7501 ggtcggtgcg ttcagcagcg tcggatcgtc ggagagcgag agatgtgtct tcgccatgca
     7561 gatcggcagt tcgcctagcc cttgctccgc gaagcgcgcg atcttctgct cggcgaccgg
     7621 gaggaagtgg acgttgtcgg cgccgtagac gcgcctagcg atcgcctgga ccttgtctgc
     7681 gatgggcgcg tccagcggat acgtgtgctc gaacgagttc gggtgcgcag ctgcgtcgat
     7741 cacagcctcc gcgagcgccg tcgcgcccgc gccgccgcgc tcgaagccct cgttcgcctc
     7801 ggcggcgtgg gcgccgtgct cgacggcaag tcggcgcacg agctcgacct cctcgtcggt
     7861 atcgcccggg aagcggttca cggcgaccac ggccttcagc ccaaagccgg cgatgatgcc
     7921 gaggtgccgc gcgaggttcg cggccccgcg ctcgatcgcg tcgagccctc cgtccggatc
     7981 gccgccgtga tgcttgagcg cgcgcgccgt cgcgacgaga acgatcgcac tcggactgag
     8041 gccgccggcc cggcagacga tgttgaggaa cttctccatg cccatgtccg aaccgaagcc
     8101 actctccgtg acgacgtagt cgccgagctt gagagcgacg cggtcggcga cgagcgagtt
     8161 gttgccgtgc gcgatgttcg cgaacggtcc gcaatggatg aacgccggct gcccctcgag
     8221 tgtctggacg aggttcggct tgagcgcgtc cttcaacagc actgtcatcg cacctgcggc
     8281 ccgcaactgc tccgccgtca cgggctcgcc ctcgtagctc tgcgcaaccg tgatcgcgcc
     8341 cagccgtgca cgcaggtcgg ccaggtcgcg cgagacggcg acgatcccca tcacctctga
     8401 ggccgcggtg atgtcgaacc cggtctggcg gacgtagccg ttggctttgc ccccgagccc
     8461 gacgacgatg tcacgaaggg ccctgtcgtt catgtccata cagcgccgcc aggtaacgct
     8521 cagcgggtcg acgccggcct ggttcccgtg caggatgtgc gcctcgagga tggtcgccag
     8581 caggttgttt gccacgccga tcgcatgcat gtcgcccgtg aagtggaggt tgaggtcctc
     8641 catggggacg acctgcgtgt ggccgccgcc ggctgcaccg cccttgatcc cgaagaccgg
     8701 cccgagcgac ggttcgcgca gacacagaac cggcttcgcg ccgactgcgc ctagcccctg
     8761 cgtgagcgag acggaggtcg tggtctttcc ctcgcccgct ctcgtcggcg tgatcgccgt
     8821 gacgcagacg agcttgccgt ccgcaacgga cgcgcgcttg tcgagggccg acaggttgac
     8881 cttcgccttg taggacccgt agaactcgat ctcgtccggc tcgaggccgg cgtccgcagc
     8941 gatctcggcg atcggccgga gcgtcgcctg ctgcgcgatc tcgaggctgg agagcatcta
     9001 gtgggagacc gccgccgcgg cgagctcgga gacaagggtg tcggtgcgga agcgctcgag
     9061 tgcgaacggg gcgatgagct gcggcgtccg ccccgttgca acgagctcgg caagcgtggt
     9121 gccgacgatc ggcgccgcct tgaacccgta ggtcccccac ccgcacgaga tcaggaatcc
     9181 gtccacttcg gtcacaccga ggatcggact gtagtcggga ctgaggtcgc acaacccggt
     9241 ccactggcgc aggatcttcg cgctctcgag ctgtgggaac agctcgagtg tgtggcgcgc
     9301 cgagtactcg aggaacggga acgtgctcgt gccgaggtac gtcgtgtacg gctcgatctc
     9361 ggcgccgatc aggaactcgc cgcggtccgt ctgggagatg tacacgtgca tctgcgacga
     9421 gacgatgatc acgtcgagga acggcttcag cggctcggtc acgaaagcct gcaggatgtg
     9481 cgtcgtgatc ggcagcggca cgccggcgag gtcggcgacg agcgtcgacc agcccgcggt
     9541 ggcattgatc acggtcccgg cctcgacgtc gccccggttt gtccgcacgc cggtgacgcg
     9601 gccgttcgac cgctcgatgc cggtcacctt ggtgtatggg tggatctcga caccgcgccg
     9661 gtcggccgca cgagcgagcc cccagacgac cgcgtcgtgg cggatgattc cgccgggcgc
     9721 gtggtagagc gcgcccatga tcggccaggt cacgtcgctc gacaggttga gctccggaca
     9781 gaggcgcccg acctcttccg catcgacgag gcggctgtcg atgccgagca gctggttcac
     9841 ctccgcccgc tcggccatcg tgatcatggc gcggtcggag tgcgcgagcg tcaggtgccc
     9901 ctgttgtgag aacaggaggt tgaagtccag ctctgcgccg agcccttcgt agagctcgac
     9961 gctgcggcgg tagaacgcag cgccctcggg agtgcggtag ttcgctcgga tgatcgtcgt
    10021 gttgcgcccg gcggcgccgg agccgatgta gctctgctcg aggatcgcga cgtcggtgat
    10081 gccgtggttc ttcgcgaggt agtaggcggt cgcgagcccg tgcgagccgg cgccgatgat
    10141 cacgacgtca tagctctcct tcaactctct gcggggacgc cacatgcgtt ccacccggaa
    10201 gatgtctctc attcgattcc ctcgagcacg tcgagcacga cctctccgac gtagtgcgcg
    10261 aactcctgtg ggaagaagat gcggaagcgc tccccgtcgc ggagcacgag cgccgacacc
    10321 tctgcgacct tcccggccgc aggcaaggcc tcgagatcga ggtcggtgag gcgccgcatc
    10381 gcgcgctcac cctcgagctc gagaccgccg agagcgccgg tcatgtcgat caccatgccc
    10441 ggaagccggg cccgcagctc ggcgcactgc tcgtacggac agaggacgag cgcgcgccgc
    10501 gacgtgattc gaacgacctc ggcgtcgacg tcgatcgcgt cgacgtcagc ccgaacctcg
    10561 atcttgccga gctgcgacag gtcacggacg gccgtcgtcc cggccagcac gcgcgcgagc
    10621 ggtgaggcga agcgaggcgc gaagagatcc tcggtccgcg ctcggtcggg agagaggaag
    10681 gcgagctcgg tcacgaccgg agctgcgccc cctcagggtc gaagaacggc ttgagtcgga
    10741 cgcgcgcgct cgctaccccg ccgttgaccc tgatctcgat ctcggtgtct tcctgcgcca
    10801 attccgggcg aacccaggcc atcccgattg cgcgtcccag ctgcgggctc aagcgggcgc
    10861 tggtgacgcg cccggcggac cggcccccga gcacgatctg tccaccctcg gccggcacga
    10921 cgccgtcggt catctcgaag ccgaccaact gctcgcggaa gccgcgctcc tgcacgtgct
    10981 cgagtgccca cttgccgacg aagtcgtcct tgtcgagctt cgcgatccac ggcatgccgg
    11041 cctcgagcac gttcgactcg gaatccgtgt cctggccgat gaggatgtgc atcttctcga
    11101 gccgcaggat gcgctggggc tcgagcccga acggacggat cccgaggtcg gcgccgtact
    11161 cgagcagggt gtcccacatg tattcgccgt acgggctggc gaaatgaagc tcgtagccga
    11221 gctcaccgac gaatccgatg cgtaggatca gacacggcac gcctgcgaca agcgtttcct
    11281 ttgcgtcgag gtacgagatg ccgtcgttgg atgtgtcgac gtcggcgagc cggccgagca
    11341 gttgcctcga acgcggcccg gcgacgttca cggcggcgag agagccggtg aggttgacgg
    11401 tctcgacgtc cagccgccag atcgcgttcc accactcgaa ccactcgatc accgtgtcgg
    11461 cgccggtgga ggtggtcgtg acgtagtact gctcgtcgcc gagacgcgcg atggtgccgt
    11521 cgtccatgat gcggcccgcg tccgtaccga ggacgccgta gcggatccgc cccaccttca
    11581 tgtcgccgaa gcggttgggg tagagcaact cgaggaaccg tgcagcatcc gggccctgga
    11641 cgaggagctt gccgagcgtc gacacgtcga tgactcccag tgcccggtgc acgttcagcg
    11701 cctcgttccc ggcatgctcg tacgaatggg cgcggcgcca gaggcccgtc cacatcatcg
    11761 tcgcgccggc ctcctcgtgc cgatgatgga tcgacgtccg cttggtgggc tcatgaccgc
    11821 ggcccgcaag caggcccagc gagaccggcg cccagggtgg gcgtgccgta gtcgtcccga
    11881 tggtggcctc gtccgtctct tgctcgcgag cgaacagccg gatcgccggc acgtggcaga
    11941 gccgtccctg gcaagggccc atcgtcacgg tcgtgtagcg cttggcgagc tcgatcgagt
    12001 cgaagccttc ggcgattgcg cgcttcatgt ccttctcggt gacgtcctca cagacgcaga
    12061 cgaagcagcg gcccgcttcg tcggcgccgt tgaaactcgc ggccggcacc gccgctggcg
    12121 ccaattctcc ggcgaccgat ccgaccgctt ccacgttcgc cggcaagtcg gagggcacga
    12181 agatcccccg accggcgttg tactcgatcc gggcgccggc ctgagcgagg agcgagtacg
    12241 ccggctggcg tccgcccgac gcgacgagca tgtcgcactg gacctcgcgg ccgtcgacga
    12301 cgacgctggt cagccggccg cggcgcccgc gtgcagcgag ttggcgcggc ggagccgagc
    12361 gcaggtcgat ggactcgacg acgctcgtgc cggctcgctg tagctggtcc gcgaccgcaa
    12421 gcccgcggtc gtcggcggac acgatcacgg cccgttcgcc cggcttcagc gaccactcgt
    12481 cgacgagtct ccgcgcggcc tccggcaggt agacgccgac gagatcgttg ccgggaaaca
    12541 ccagcggctg ctcgagcgct cccgttgcca cgacgatccg ctctgcgcgg aaacggtaga
    12601 ggacactgcc ggcgtcgacg gggacgagac cgccctcgta gatcccgatc gcccgcgccg
    12661 gggcgagcac ctcgaagtca ccgttcgcac cggggacccc gcgctcgtcg acgagcacca
    12721 cctgccgacc ctcggccgcg gcggagcggg ccgcctcacg gcccgcgcgg ccgccgccga
    12781 tcacgagcac ctccgcacgg cgatgctcgg tgtcgaaccg gcgcgaccgg cccgcgtgtt
    12841 tgtccagccg ccccaggccg gcgatattcc tgaggaactt ctcgtacacc ggccacatgc
    12901 cacgcgggcg gatcatcgtg cggtagtaga agccgaccgg agtaaacggg cccccgaagt
    12961 ggtcgacggc ggcaagcagg tcgcgctcga gtgagcccag gacgttctga gcatgcactt
    13021 gcgcgccctc gcgcaccggt tccacacaga cgcgcacgtt cggatcgccg tccacctgca
    13081 tcatgcagtt cgggcacgcg ccggaacagc agagcaaccc gcggcggcgg tggtacttga
    13141 agctgcgaga gaacacgcgt cgcccggagg cgaacagcgc agagccgatc gtgtcgccct
    13201 cgaacccctc gaccggacgg cctccgaaga agaaccggat tggtcgcgcg cggtcgatgc
    13261 gctcgttcgg ctgttgcgga agcctcaccg tgcggccggc gtctcggccg aggcctgcgg
    13321 caattccgtg tgcagcacct cgttcgtgcg cgtgtcgcgg tcggcgagga accagagctc
    13381 acagccgaag cggtggtacc accattcgcg ctggacgccg gcgacgttgc ggcgaaagta
    13441 gacgtacgcc gagagctcct gctgcgaagg cgtcgtgcgc ggccggtgcg tcacctcgcc
    13501 cgcgtaggca aactcgttca cgtcgcgcgg gccgcagttg gggcagggaa gcaggaacga
    13561 catcgctcgc gctacgccga gtggcgtccc ggaatccagt cggtgccggc gagcggcacg
    13621 cgcgccatcg ccgcggcctc gaccgtcagc gcgacgagat cctccggctc gaggttgtga
    13681 acatgcgact tgccacacgc gcgcgcgagc gtctgcgcct cgagggtgag gacgcgcaag
    13741 tagttcgcga tccggcgacc gccgagcaca ggatcgagac gggccgagag ctcgtcgtcc
    13801 tgcgtcgaga tccctgctgg gtcgcggccc gcctgccagt catcgtagaa gccggcagcg
    13861 ctgccgagct tgcggtactc ctcctcgtac tcggggctgt tgtcgccgag cgagatcaag
    13921 gcggccgtcc cgatcgagac cgcgtcggcg ccgagcgcga gcgccttcgc gacgtcggcg
    13981 ccggtgcgga tgccgccgga gatgatcagc tgcaccttcc ggtgcatgtc gagctcctgg
    14041 agggcgtgca cggcaaggcg cgttgccggg agcgtgggta tgccgacgtg ctcgatgaag
    14101 acgtcctgcg tcgcggccgt tccgccttgc atgccgtcca cgacgatgac gtcggcccct
    14161 gccttgaccg ccagctgcac gtcgtagtga gtgcgcgtcg ctccgatctt gacgaagatc
    14221 ggcttctccc agtccgtgat ctcgcgcagc tcgtggatct tgatctcgag gtcgtccggt
    14281 cccgtccagt ccggatggcg ggacgcgctg cgctggtcga tcccctgcgg cagagagcgc
    14341 atggccgcga cgcggtcgga gatcttgtgg cccaacagca ttccgccccc gccaggcttc
    14401 gcgccctggc cga
//
LOCUS       DASVEU010000264         5818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U2070 k141_1358230,
            whole genome shotgun sequence.
ACCESSION   DASVEU010000264 DASVEU010000000
VERSION     DASVEU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961390
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:32:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,235
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,194
            CDSs (with protein)               :: 2,194
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358230"
                     /isolate="SMAG_U2070"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..765
                     /locus_tag="VF225_11140"
     CDS             106..765
                     /locus_tag="VF225_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7145851.1"
                     /translation="MSEDSVPSQVESTGTTGRPTLDPPAAEARERIVRTAYELFYRDG
                     VSAVGVDRIVAEAGVAKTTLYRHFRSKDDLVVAVLERHEDLWTSGWIEREVEHRATSP
                     QERVPVLFEVFYEWLRDDSYEGCLFINSLLEIHGRSEVIRASSVAALEHVHALVARFA
                     REAGIGDPGSFAHQIQIIMRGGIIAALEGQFEAVDEARSLARKLWEQESSSGIDTKNR
                     T"
     gene            complement(933..1277)
                     /locus_tag="VF225_11145"
     CDS             complement(933..1277)
                     /locus_tag="VF225_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7145852.1"
                     /translation="MALFLIIEPHEEIRPLYAAVIRGLGHEAVVFESGAIAKPDALLV
                     EPAYPESFEAARRLRLAHPDLPIVCASIHEPIREEVQVLRPSTYLLKPFSLVELRDAL
                     RLALSPRELLPD"
     gene            1336..2031
                     /locus_tag="VF225_11150"
     CDS             1336..2031
                     /locus_tag="VF225_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145853.1"
                     /translation="MTRARVLVIDDDGDVRALVAGLLDRAGYEVNEASDGRDGLRSVF
                     SDRPDLVLLDVTMPGLDGWATLERIRELTDVPVLMLTARSDELEKVRGLKAGADDYMT
                     KPFGRQELLARVEALLRRKRNAQEELPTVYADDAITVEFAQRSARSDGVDLRLTPLEF
                     RLLSAFVQHPNQVLSREQLLELAWGDPYGVSEDQVKLYVGYLRKKLGANVGTESPIET
                     VRGFGYRYRPVRL"
     gene            complement(2028..2420)
                     /locus_tag="VF225_11155"
     CDS             complement(2028..2420)
                     /locus_tag="VF225_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7145854.1"
                     /translation="MTHRESPLVLVADDEEDIRALVAFRLKRAGYDVITAADGEEALL
                     LATTRLPDLAVLDMMMPKATGLEVTHSMREQAATKDIPVILLTARAQEVDVQRGFEAG
                     ADDYVKKPFSPQDLQARVHALLERSAAS"
     gene            complement(2448..3062)
                     /locus_tag="VF225_11160"
     CDS             complement(2448..3062)
                     /locus_tag="VF225_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145855.1"
                     /translation="MDSQRISCVVADDHPALLAAVSDFLEEHGYDVVAACANGRDVLA
                     AVRALTPEIAVLDYRMPGGPGADLIKDILVASPNTRIVVYTGDGTTELAAEVLGAGAR
                     AILLKQAPLDDLLRALRAAAGGVRYVDPLLAPDSRSASVTLTKRERQVLELVAEGLGQ
                     QEIGRRLSIGTETVRAHLQKVRKRLSATTSTQAVAIALRLGLLE"
     gene            3243..4430
                     /locus_tag="VF225_11165"
     CDS             3243..4430
                     /locus_tag="VF225_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7145856.1"
                     /translation="MDRIRVLVADDEPAVRAVLAELVSAEATLELVGTAKDAEEAIEI
                     AVRERPDVALLDVKMPGGGGPRAAREIRVSSVQTRVVALSAYEDRASVLAMLRAGAVG
                     YLVKGTRAAEIVKLIHDVVRGEVVLSAEVMGDILEELTGRLQVEGDEEELLRAQAARI
                     REVLDGDGLTMVFQPIVDLDADLVVGVEALARFPSIPERPIQSWFAEAAVVGLKGELE
                     LAAVAAALAVFPEVPAGLYLSINATPDSLASDGLTELLATAPAGRLVLEITEHAPVHD
                     YEALNAAMEWMRGRGIRLAVDDAGSGFASLRHILQLAPDIIKIDNALTRNVYMDPARR
                     ALAAGLISFAAELGATVIAEGIENRQELETLRALGVRYGQGFYLGRPGPIADVMSIGD
                     HAL"
     gene            4475..5113
                     /locus_tag="VF225_11170"
     CDS             4475..5113
                     /locus_tag="VF225_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7145857.1"
                     /translation="MHEPTSSDPVRAVVADDHPAVLRAVCDVLIDVGVDVVGRASNGE
                     EAAAKIEATQPTVAVVDVRMPRLTGIEIAARVSRTAPETSVILYTAYGDRALLTEALD
                     SGVRGFVLKEAPMQDLTRAVDMVAGGAVYVDPVLAGVLASAEAERIPQLTKREREVLR
                     LLADGLTNEEIGKRLFLSPETVRTHVQKAMRRLEADTRTQAVALALRQSLIA"
     gene            complement(5120..>5818)
                     /locus_tag="VF225_11175"
     CDS             complement(5120..>5818)
                     /locus_tag="VF225_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7145858.1"
                     /translation="LHLTTPNIPGLEGRAFGRELAELLGGVVTLENDVNLAAVGERWA
                     GVARGVDDFAFLSVGTGMGMGLVLGGELHRGNHGAAGEVDWALAGLAEDADPSALGVA
                     TLAGRIAPPGSAGTAVAPPYDARTIFAAARRGDLLARRVVEEVARRIAAHIAPIAAVA
                     DPALVVLGGGLGTNGDLLLTPVRRLLAEWIPYPPRVEISSLGEAAVLMGALAVGLRSA
                     LDNVFVNRPQSARA"
BASE COUNT          958 a         1874 c         1998 g          988 t
ORIGIN      
        1 atacacctgt cgtcttgatc tcgcaatggc gaatcctgat caattggagc tacagacagg
       61 tctgtagcgc aagcaggtta gcatgctgcc taccgtgaga gccctgtgag cgaagactcg
      121 gtgccgtccc aggtcgagag caccgggacg acaggccggc cgacgctcga cccgccggcg
      181 gcggaggctc gtgagcggat cgtgcgtacc gcctacgagc tcttctaccg cgacggggtc
      241 agcgccgtcg gcgtcgaccg tatcgtcgcc gaggcagggg tcgcaaagac caccctctat
      301 cggcatttcc gctcgaagga cgacctggta gttgccgtcc tcgaacgtca cgaggatctc
      361 tggacgtccg gttggatcga gcgcgaagtc gagcaccgcg cgacttcacc tcaggagcgg
      421 gtacccgtcc tcttcgaggt cttctacgag tggttgcgag acgacagcta cgaggggtgt
      481 ttgttcatca actcactgct cgagatacac ggccgctccg aagtaattcg cgccagttcc
      541 gtcgccgcgc tcgagcacgt ccatgcgctc gtggcgcgct tcgcacgaga ggccggcatc
      601 ggcgaccctg gcagtttcgc gcaccagatc cagatcatca tgcgaggagg gatcatcgcc
      661 gcgctcgagg gtcaattcga ggccgtggac gaggcgcgct cactcgcgcg caagctctgg
      721 gaacaagaaa gctcgtccgg aatagacacg aagaaccgaa cctgatccgg cggcagaagg
      781 gtgatctgct cgaccagctt cggggatggc ggccggcgag gcgtgctccg atgttccctg
      841 tgaaaggtcg aactgtgatg aaccttgaac tcgccagccg tcgaccctgc gcgcggggcg
      901 gcaactccat ccgccccgtg caacccgttc gctcagtcag gcaagagctc acgtggcgag
      961 agagccaacc gcaatgcgtc cctgagctcg acgaggctga acggcttgag cagatacgtg
     1021 ctcggccgca gaacctgaac ctcctcccgg atgggctcgt ggatgctggc gcacacgatc
     1081 gggaggtccg ggtgagcgag ccgcagccgc cgggccgcct cgaacgactc ggggtaggcc
     1141 ggctcgacca gaagcgcgtc cggcttggcg atggcgccgc tttcgaagac gaccgcctcg
     1201 tggcccaggc cgcggatgac tgccgcgtac aagggccgaa tttcttcgtg cggttcgatg
     1261 atcaggaaaa gggccatggg cacgttctcg tagaccggcg gatgttttcc agggctgctt
     1321 gcgtatgagg ctcgaatgac acgcgcgcgc gttctcgtca tcgacgacga cggtgacgtt
     1381 cgtgctctcg tggccgggct gctggaccgt gccggctacg aggtgaacga ggcctcggac
     1441 gggcgcgacg gcttgcgttc ggttttctcc gaccgtcccg acctggtgct cctcgacgtg
     1501 acgatgcccg gtctcgacgg ctgggccaca ctcgaacgga tccgtgagct caccgacgtg
     1561 ccggtgctca tgctcaccgc gcgttccgac gagctcgaaa aggtgcgagg cctgaaggcg
     1621 ggagcggacg actacatgac gaagccgttc ggccgccagg agctgctagc ccgagtggag
     1681 gcgcttctcc gccggaagcg aaacgctcag gaggagttgc cgaccgtcta tgccgacgat
     1741 gccatcacgg tcgagttcgc gcagcggtcc gctcgatcgg acggcgtcga ccttcggctg
     1801 acaccactcg agttcaggtt gctgagcgcc ttcgtccagc atccgaacca ggtcctcagc
     1861 cgagagcagc tgctcgagct cgcgtggggc gatccctacg gtgtctctga ggaccaggtc
     1921 aagctgtacg tgggctacct gcgcaagaag ctcggggcga atgtcggaac ggagtctccg
     1981 atcgagactg tgcgcggctt cggttaccgc taccggcccg tccgcctcta gctcgcagcg
     2041 gagcgttcga gcagcgcgtg aacgcgcgcc tggaggtcct ggggactgaa gggtttcttg
     2101 acgtagtcat cggcgccggc ctcgaaacca cgttgtacgt cgacttcctg cgcgcgtgca
     2161 gtgaggagga tgaccggaat gtccttggtc gccgcctgct cccgcatgct gtgcgtgacc
     2221 tcgagcccgg tcgccttcgg catcatcatg tcgagcacag cgagatcagg caggcgcgtg
     2281 gtcgcgagca ggagcgcttc ttccccgtcg gccgcagtga tgacgtcgta gccggcgcgc
     2341 ttgaggcgga acgccacgag agcacgaatg tcctcctcgt cgtctgccac gagtacgagg
     2401 ggtgattctc tgtgggtcat gcggcaacct tccttgggtc gggaaatcta ctcgagtaga
     2461 cccagccgca gggcaatcgc cacggcctga gtacttgtcg ttgccgagag tcgcttgcgc
     2521 accttctgga ggtgcgctcg aaccgtctcc gtgccgatcg acagcctgcg cccgatctct
     2581 tgctggccca ggccttcggc gaccagctct aggacttgcc gttcccgctt ggtcagggtg
     2641 acgcttgcgg agcgcgagtc cggggccaga aggggatcga cgtaacgtac gccaccggcg
     2701 gccgctcgaa gggcacgcag cagatcgtcg agaggtgcct gcttcagcag gatcgcgcgt
     2761 gctccggcgc cgagaacctc tgccgccagc tcggtcgtcc cgtcgccggt gtacaccacg
     2821 atgcgcgtgt tcggggaagc tacgagaatg tctttgatca aatcggcgcc gggcccaccg
     2881 ggcatgcggt agtcgaggac ggcgatttcg ggcgtcaacg ccctgacagc cgctagaacg
     2941 tcgcggccgt tggcacacgc ggcgacgacg tcgtagccgt gttcctcgag gaagtccgag
     3001 acggctgcga ggagcgcggg gtggtcatcg gcgaccacgc agctgattcg ctgtgagtcc
     3061 atctctgtgt tctcggtctt ggcaagctcc cggccaatcg ccgataccgc gagtagggcc
     3121 tacgctgatc tggggactcc ccgtcaccct tttcgagggt gacgcctgcc ggcggtcggt
     3181 agcatcgcaa cgctgcgcga ccgcggtgat ctcttttttg agcaaggccg agaggagtgc
     3241 tcatggatcg aatccgcgtg ctggtggcgg acgacgagcc ggctgttcgt gcggtcctcg
     3301 cggaactcgt ctcggctgag gccacgctcg agctggtggg caccgccaag gacgccgaag
     3361 aggcgatcga gattgctgtc cgcgaacggc cggacgttgc gctcctggac gtgaagatgc
     3421 cgggcggcgg cgggccgcgt gcggcccgtg agatcagggt gtcctcggtc cagacgaggg
     3481 tggtcgccct ctcggcctac gaggaccggg ccagtgtgct cgcgatgctg agggccggtg
     3541 cggtcggata cctggtcaag ggcactcgtg ccgcagagat cgtcaagctg atccacgacg
     3601 tcgtccgcgg cgaagttgtt ctctccgccg aggtgatggg cgacatcctc gaggagctca
     3661 ccggcaggct gcaggtggaa ggagacgagg aagagctcct gcgcgcgcag gccgcgcgga
     3721 tccgtgaggt gctcgacggc gacgggctga cgatggtctt ccagccgatc gtcgatctcg
     3781 acgcggacct tgtcgtcggc gtcgaggctc tcgctcgttt tccctccatc cctgagcggc
     3841 cgatccagag ctggttcgca gaggccgcgg tggtcggatt gaagggcgag ttggagctgg
     3901 ccgcggtggc cgcggcgttg gcagtgttcc ccgaggtgcc tgcgggcctc tatctctcca
     3961 tcaacgcgac gcccgactcg ctcgcctcgg atggcctcac cgagctgctc gcaaccgcgc
     4021 ccgcgggtcg tctcgttctc gagatcaccg agcacgcgcc ggtacacgat tacgaagcgc
     4081 tgaatgcggc catggagtgg atgcgcggcc gcgggatccg acttgcggtc gacgacgccg
     4141 gctccgggtt cgccagcctg cgccacatcc ttcagctcgc tcccgacatc atcaagatcg
     4201 acaacgccct gacgcggaac gtctacatgg atccagctcg ccgcgccctc gcggcgggct
     4261 tgatctcctt cgcggcagag ctcggcgcca ccgtcatagc cgagggcatc gagaatcggc
     4321 aggagctcga gacgttgcgt gcgctcggtg tccgttacgg gcaagggttc tacctcggcc
     4381 gccccgggcc gatcgcggac gtgatgtcga tcggcgatca cgctctttag cgagtcgacg
     4441 gcctccgggg accgctctgc gtatcctcct ctgagtgcac gagccaacca gcagcgaccc
     4501 ggttcgcgcg gttgtcgccg acgatcatcc agcggtgctc cgtgctgtct gcgacgtctt
     4561 gatcgacgtt ggtgtcgatg tcgtggggcg ggcgtcaaac ggagaggagg cggctgccaa
     4621 gatcgaggcc acgcaaccga ccgtcgcagt cgtcgacgtc aggatgccgc ggctgaccgg
     4681 gatcgagatc gcggcgcgag tttctcgcac ggcccctgag acctccgtca tcctctacac
     4741 cgcgtacgga gatcgggcgc tcctgaccga ggcgctcgac tcgggcgttc ggggcttcgt
     4801 gctgaaggag gcgccgatgc aggatctgac ccgagcggtc gacatggtcg cgggcggagc
     4861 cgtctatgtc gatccggtcc tggcgggagt gctcgcaagc gcggaagcgg aacgtattcc
     4921 ccagctcacg aaacgggagc gcgaggtgct gaggctcctg gcggacggtc tgacgaacga
     4981 ggagatcggc aagcggctgt ttctctcgcc ggaaaccgtg cgcacgcacg tccagaaagc
     5041 aatgcgcagg ctggaggcgg acacgcggac acaggctgtc gcactggcgc ttcgccagtc
     5101 gctcatcgcc tgaccaccgt caggctcgcg cggactgcgg gcgattcacg aagacgttgt
     5161 cgagcgcaga gcgcagcccc acggccaggg cgcccatgag cacggcggct tcgccgaggc
     5221 tcgagatctc gacccgcggc gggtaaggga tccactctgc cagcagccgc cgcaccggtg
     5281 tgagcaagag gtcgccgttc gtgccgaggc cgccgccgag gacgacgagc gcaggatccg
     5341 cgaccgcagc gatgggagcg atgtgggcgg cgatgcgtcg agcgacctcc tcgacgaccc
     5401 tgcgtgcgag caagtcgccg cgccgtgctg ctgcgaagat cgtcctggcg tcgtaaggag
     5461 gagcgactgc cgttccggcc gagccggggg gcgcgatccg gccggccagg gttgcgaccc
     5521 ctagcgccga ggggtcggca tcctccgcca ggccggcaag cgcccagtcc acttcgccgg
     5581 ccgcgccatg attgccgcgg tgcagctccc cgccaagcac gagccccatg cccataccgg
     5641 tgccgaccga gaggaaggcg aagtcgtcga cgccgcgcgc gacgccggcc caacgctcgc
     5701 cgaccgctgc gagattgaca tcgttctcga gcgtgaccac gcccccgagc agctcggcca
     5761 actcgcggcc gaaagcgcgg ccctcgaggc ctgggatgtt cggcgtggtc aggtgcag
//
